LOCUS       DASTJJ010000001         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1467778, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000001 DASTJJ010000000
VERSION     DASTJJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467778"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1062)
                     /locus_tag="VFR02_00005"
     CDS             complement(<1..>1062)
                     /locus_tag="VFR02_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HET9868868.1"
                     /translation="TLINAGTLSIDSAGDLGTGNLTLNGGSLAATSTLTLAQSVTLAP
                     GGGTFNVGSGKTLTLSGGFGAGSGTLTTAGLGTLNLTGSSLYTGDTVLGSGSTLQFSL
                     PSDQAYSGNISGGGNLAVAAGSHTLTLGGTNTYTGTTSLLSGTLSLTGVDALGTGASS
                     SSGLTLGSGTTLGLNFSGTLSNTHTITLSGAAGLAGSSTSTLNNNISLGGTLANADTG
                     TLTLGGTLTGTGGLTQNSVGTLVLTGANSYSGPTSLTNGTLVAGGNSALGTGNLVLNG
                     GTLDSQSAVTLSNNMSLTGNTQVGGSNDLTLNGSLSMGGGATLTDTDTGNVKLGGTLS
                     GTGSLTEDTTGSLTLSGNN"
BASE COUNT          165 a          325 c          384 g          188 t
ORIGIN      
        1 tgttgttgcc cgaaagcgtc aaactgcccg tggtgtcctc ggtcaaacta ccagtccccg
       61 aaagggtccc gcccaatttc acgttgcccg tgtccgtgtc ggtcagggtg gcgccgccgc
      121 ccatggagag gctgccgttc agagtgaggt cgttcgatcc cccgacctgg gtgttgcccg
      181 tcagggacat attgttggaa agggtgacgg cggattggga gtccagcgtg ccgccgttga
      241 ggaccaggtt gcccgttccc agggcgctgt tgccccccgc cacgagcgtc ccgttggtca
      301 ggctggtcgg gcccgagtag ctgttggcgc cggtgaggac cagggtcccc acgctgttct
      361 gggtgaggcc gcccgtgccc gttagcgtcc cgcccagggt gagggtcccg gtgtccgcgt
      421 tggccagggt gccgccgagg gagatattgt tgttcagggt cgaggtcgag cttcccgcca
      481 ggccggcggc gccgctcaag gtgatcgtat gggtgttgga cagggtgccc gagaagttca
      541 ggcccagggt ggtgccgctc cccaaggtca ggccgctgga ggagctggcc cccgtgccca
      601 gggcatccac gcccgtgagg ctcagggtgc cggaaagaag actcgtcgtt cccgtgtagg
      661 tattggtccc gcccaaggtc agcgtatgcg agccggcggc cacggccaaa ttgcccccgc
      721 cagaaatgtt acccgaatag gcttggtcgg agggcaggga gaattgcagg gtactcccgg
      781 accccagcac ggtgtcgccc gtatagaggc tggacccggt cagattcagg gtccctaggc
      841 ccgccgtggt gagagtgccg gatcccgcgc cgaaaccccc ggagagggtc agggtcttcc
      901 ccgaacccac gttgaaggtg ccgccgccgg gggccagggt gacgctttgg gccagggtga
      961 gggtggaggt ggccgccaaa gagccgccgt tcagggtgag gttacccgta cccaagtccc
     1021 cggccgagtc gatggaaagc gtaccggcgt tgatcagcgt ac
//
LOCUS       DASTJJ010000002         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_734092, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000002 DASTJJ010000000
VERSION     DASTJJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734092"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFR02_00010"
     CDS             complement(<1..710)
                     /locus_tag="VFR02_00010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9868869.1"
                     /translation="MSAADGMTAERSRVLLTGGGTGGHVFPGIALAQELGRRGGVEVL
                     YVGKQSGPESRWVPAAGLPFEGIPASGFPRELGPRLFTFWGDLVRGFARSRAILRRFK
                     PQAVFSTGGYVSVPVSLAAWLSGVPVLLFEPNVAPGWAARILAFLSKRVFTGFRRTAE
                     TFASSKSVWTGIPVRREILEAQKGEALGHFGLDAQKTTLLLLGGSQGARALNHMMTGA
                     IRELNEGEDPLQVLMMTG"
     gene            complement(707..1855)
                     /gene="ftsW"
                     /locus_tag="VFR02_00015"
     CDS             complement(707..1855)
                     /gene="ftsW"
                     /locus_tag="VFR02_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9868870.1"
                     /translation="MNTKTRQLESPDFMLFLITLILTASGLIMVYSASAVLAHAQYGN
                     SYYFIARQFLWVLVGTAAAWAVSRVDLQTLRGLAIPALLVGFLLMALVCVPGIGRTVG
                     GAQRWFRLGPFSFQPSEVMKIIMVFYLADSLDRRRHLLVNFSGLLPYLVILGVTMVFL
                     EKQHDFGTAVLLALVTILMLFLAGMPWGYIVIPLSILIPVFIFLVESASYRMRRITAF
                     LNPWDDPQGSGFQLIQSLIAVGSGGPFGVGLSNSSQKLFYLPAPHTDFIFAIICEELG
                     LVGGGALVAFFAFFILRGLKVAETAGARPGGFFLSLLASGITALIGFQALVNLGVVTG
                     LLPTKGIPLPLISYGGSSMVFTLVSIGILFNISRGLKLPFPLGGKRTV"
     gene            complement(2213..>2532)
                     /locus_tag="VFR02_00020"
     CDS             complement(2213..>2532)
                     /locus_tag="VFR02_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9868871.1"
                     /translation="LGGRDKAGDFRTLIPLVREKATRVVAFGECKAKVVQQLAGAATV
                     VEADTLEEVVNGAFTACEKGGTVLFSPGCASFDMFENYEDRGRRFKALVAKLAESKSH
                     AKA"
BASE COUNT          511 a          851 c          798 g          372 t
ORIGIN      
        1 tggccggtca tcatcaacac ctgcagggga tcctcgccct cgttcagttc ccggatggcg
       61 ccggtcatca tgtggttcaa ggcgcgggcg ccctggctgc cgcccagcag cagcagggtg
      121 gtcttttgcg cgtccaaccc gaaatgcccc agggcttccc ccttttgggc ctccaggatc
      181 tcccggcgaa ccgggatgcc cgtccagacc gacttggagg aagcgaaggt ctcggccgtc
      241 cgccggaaac ccgtgaagac ccgcttcgac aggaaggcca ggatccgggc ggcccacccg
      301 ggggccacgt tgggttcgaa caacagcacg ggcacgcccg acagccaggc cgccagggac
      361 accggcacgg acacgtagcc gccggtggaa aagaccgcct ggggcttgaa gcgcctcagg
      421 atggcccggg accgggcgaa gccccggacc aggtcgcccc agaaagtgaa caggcgcggg
      481 cccagttccc ggggaaaacc gctggccggg atgccctcga agggcagtcc cgccgccggc
      541 acccaccggc tctcgggccc ggactgtttg cccacgtaaa gcacctccac cccgccgcgc
      601 cggcccaatt cctgggccaa ggcgatgccc gggaacacgt ggccgcccgt gccgccgccg
      661 gtcaaaagca ccctcgacct ttccgccgtc atgccgtccg ccgcgctcat accgtcctct
      721 tcccgcccag ggggaaaggc agcttcaacc cccgggaaat gttgaacagg atgccgatgg
      781 agaccagggt gaagaccatg gaggacccgc cgtaggagat gagcggcagg gggatgccct
      841 tggtgggcaa cagccccgtg accaccccca ggttcaccag ggcctggaag ccgatgagcg
      901 ccgtgatgcc ggaggccagg agggaaagga agaaaccccc cggccgggcc cccgcggtct
      961 cggccacctt caggccccgc aggatgaaga aggcgaagaa ggccaccagc gcccccccgc
     1021 ccaccaggcc cagttcctcg cagatgatgg cgaagatgaa atccgtgtgg ggggcgggca
     1081 ggtagaagag cttctgggag ctgttggaaa gccccacgcc gaagggcccg cccgagccca
     1141 cggcgatcag gctctggatg agctggaacc ccgacccctg cgggtcgtcc caagggttga
     1201 ggaaggccgt gatgcggcgc atgcggtagc tggccgactc caccaggaag atgaagaccg
     1261 ggatgaggat ggacaggggg atcacgatgt agccccaggg catgcccgcc aggaacagca
     1321 tgaggatggt caccaaggcc agcagcacgg ccgtgccgaa gtcgtgctgc ttctcgagga
     1381 agaccatggt gacgcccagg atgaccaggt agggcaggag cccggagaaa ttcaccaaca
     1441 ggtgccggcg ccggtccagc gaatccgcca ggtagaagac catgatgatc ttcatcacct
     1501 ccgagggctg gaaagagaag ggccccaggc ggaaccagcg ctgggcgccc cccacggtgc
     1561 ggccgatgcc cggcacgcag accagggcca tcagcaggaa ccccaccaac agggccggga
     1621 tggccaggcc ccgcagggtc tggaggtcca cccggctcac ggcccaggcc gcggcggtcc
     1681 ccaccagcac ccagaggaac tggcgggcga tgaaatagta ggaattgccg tattgggcgt
     1741 gggccagcac ggccgacgcg ctgtagacca tgatcagccc cgaggcggtc aggatgagcg
     1801 tgatcaggaa cagcatgaag tccggcgatt ccagttgtcg ggtcttcgtg ttcaaaacga
     1861 actccttaaa aatttcaaga aaccatccag gtccaaagac tgattcaggc accgtcgtcg
     1921 cgtggaaaat cccggcggcg actcagaaac cacttcgtct gccgtggccg ccggaaaagc
     1981 ccggcggcga ctcagaaatg accttttatc agcaacacat aacaatgagg tcatttcttc
     2041 gttgccattc agctccgcct catggcgact ccaggatgac ctcgatcaat gttcaaccta
     2101 tgggagtcat ccttccgttc ggatcctaag tctggcgtcg tgggtgtgga aaggcgttgt
     2161 aggtgttggt cttagaatcc gaacgcaaaa atgatccccg tccctgcctt tctcacgcct
     2221 tggcgtgcga tttgctctcg gccagcttcg ccaccagggc cttgaaccgg cgcccccggt
     2281 cctcgtaatt ctcgaacatg tcgaagctgg cgcacccggg cgagaaaagc accgtgccgc
     2341 ccttctcgca ggccgtgaag gccccgttca ccacttcttc cagcgtatcg gcctccacca
     2401 cggtggccgc gcccgccaac tgctgcacca ccttggcctt gcattcgccg aaggccacca
     2461 cccgggtggc cttttccctc accagcggga tcaaggtccg gaaatccccg gccttgtccc
     2521 tgccccccag ga
//
LOCUS       DASTJJ010000003         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_978838, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000003 DASTJJ010000000
VERSION     DASTJJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978838"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..414)
                     /locus_tag="VFR02_00025"
     CDS             complement(124..414)
                     /locus_tag="VFR02_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868872.1"
                     /translation="MGMLLDMTAYLLTLLLHLAALLVALDWLFHRLPGAGLHPLRRAL
                     FRLLFPLRRWGDSLGLRRDNMDFTPVFLIALMLGVGRFGLPWLALLGFSLRG"
     gene            complement(423..1244)
                     /gene="proC"
                     /locus_tag="VFR02_00030"
     CDS             complement(423..1244)
                     /gene="proC"
                     /locus_tag="VFR02_00030"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9868873.1"
                     /translation="MSLKHQTVLLGFGSMGGALAKGWIQNKVLKPSQITAVSLDPAKR
                     QETARQLKVKLVTDPSPYLPKADLILLAVKPQQLKALLGKVGGLFPRRALVLSVAAGV
                     TTQQIESRLPQGCPVVRVMPNTPSTLGAGMSAVAAGSRATASDLKLAQALLSAVGRSV
                     EVKESLMDLVTALSGSGPAYFFKMIEALVAAGEKGGLDGPTATLLAAQTAYGAARMVL
                     ETGKSPEALRVQVTSPGGTTEAGLTVLDQRGFGGALEAAVQAATQRGAELRRMNE"
     gene            complement(1573..2265)
                     /locus_tag="VFR02_00035"
     CDS             complement(1573..2265)
                     /locus_tag="VFR02_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9868874.1"
                     /translation="MDIRARIQDVKDRIAAAAGRSGRDPRSVTLLAVTKTFPVEALRQ
                     AHAAGLRLFGENRVQEALVKQPLLPADIEWHLIGQLQTNKINKVLGRFGLIQSVDSLH
                     LAKALSARLTEDQEVLLEVNTSGEASKAGFAPQELPRAFDEALGLPRLKVRGLMTVGP
                     LTQEGEARRRAFRTLRSLFDGVKGRAPEPAAFSILSMGMSGDFEEAIEEGSTLVRVGS
                     ALFGERPAPKKD"
BASE COUNT          441 a          767 c          711 g          388 t
ORIGIN      
        1 acctgcaggc gccaacggcc ggccgccagg cccagccaac gcgaaacggg ggcccccggc
       61 ttcacccgca gcgccaggga gcaaaaaccc ctgccttgcc ggacggccgg cgtttccacg
      121 gcctcaaccc ctcaaggaga aacccaggag ggccaaccag ggaagcccga aacggccgac
      181 ccccagcatc agggcgatca ggaaaaccgg ggtgaagtcc atattgtcgc ggcgtaaacc
      241 caggctgtcc ccccaacggc gaagcgggaa caggaggcgg aaaagcgcgc gccgcaaggg
      301 atggagcccc gctccgggca gacggtggaa cagccaatcc agggccacca agagggccgc
      361 caggtgcaat aagagggtca acagataggc cgtcatatcc agtaacattc ccatggtccc
      421 ccctattcgt tcatgcggcg caattcggcg ccccgctggg tcgcggcttg gaccgcggcc
      481 tccagcgccc cgccgaatcc ccgttggtcc aggaccgtca acccggcctc cgtggtgccg
      541 cccggggaag tcacttgaac gcggagggct tccggactct tccccgtttc cagcaccatg
      601 cgggccgcgc cataggccgt ttgggccgcc aagagggtgg ccgtcgggcc atccaatccc
      661 cccttttcgc ccgcggccac cagcgcctcg atcatcttga aaaagtaggc cggtccgctc
      721 ccggacaggg ccgtgaccag gtccatcaag ctttccttca cttccaccga ccgtcccacc
      781 gccgaaagca gggcctgggc cagcttcagg tccgaagccg tggcacggct tccggcggcc
      841 accgccgaca tgcccgcccc caaggtcgag ggcgtattgg gcatgacccg caccacggga
      901 catccctggg gcagacggga ctcgatctgc tgggtggtga cgcccgccgc caccgacagg
      961 accagcgcgc ggcgtggaaa aaggcccccc accttgccca aaagcgcctt gagttgttgg
     1021 ggcttcacgg ccaaaaggat gaggtccgct ttgggaagat agggggaggg atcggtcacc
     1081 aatttgacct tcaactggcg ggccgtttcc tgccgcttgg cgggatcgag ggaaacggcg
     1141 gtgatctggg agggtttcag caccttgttc tggatccatc ccttggccag ggcgccgccc
     1201 atggaaccga atcccaacag cacggtttgg tgttttaagg acaagtggtt tctcccggcc
     1261 acattctacg cccgtttttg ggcgggccaa aggccttttg caaaaggcct ttggctctgc
     1321 atttcaaggc agaccaaatg gcttttgcaa aagtcctttg gccctgcatc ccaaggcggg
     1381 ccaaaggcct tttgcgaaag accccgggcc tagaagtcat ttcaaaacct cttttaccgc
     1441 agagacgcag aggcgccaag tgaagacaag gggcgaaaac cggggaaata aacattattt
     1501 ttcctgcgcc cccgcgtctc tgcggttaaa aattttcgct tttagggttt tgaaacgggt
     1561 tctagtcggc tttcagtcct tcttgggcgc gggcctttcc ccgaaaagcg cgcttcccac
     1621 ccgcaccagg gtcgatcctt cctcaatggc ttcctcaaag tccccgctca tgcccatgga
     1681 aaggatggaa aatgccgcgg gctccggggc ccggcccttc actccatcga agagggaccg
     1741 caaggtccga aaggcccggc gacgcgcttc cccctcctgc gtcaaggggc ccaccgtcat
     1801 gagcccccgg accttcaggc ggggcaagcc aagcgcctcg tcgaaggccc ggggaagttc
     1861 ctggggcgca aaacccgcct tggaggcttc ccccgaggtg ttcacttcca gcaggacttc
     1921 ctggtcctcc gtcagacggg cggacaaggc cttggccaaa tgaagcgaat ccaccgattg
     1981 gatgagcccg aaccgaccca agaccttgtt gatcttgttc gtctggagtt ggccgatgag
     2041 gtgccattcg atgtcggcgg gcaaaagggg ctgtttgacc agggcttcct gcacccggtt
     2101 ctcgccgaaa agacgcaggc ccgcggcatg ggcttgtcgg agggcctcca ccgggaaggt
     2161 cttggtgacg gccaacaggg tcacggaccg ggggtcccgg ccggaccggc cggccgcggc
     2221 ggcgatgcgg tccttcacgt cctggatccg ggcccggatg tccatcgtca ccctccggcc
     2281 tggcggggac tcaggcctgc ggcttca
//
LOCUS       DASTJJ010000004         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_611891, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000004 DASTJJ010000000
VERSION     DASTJJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611891"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1053)
                     /locus_tag="VFR02_00040"
     CDS             complement(<1..>1053)
                     /locus_tag="VFR02_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HET9868875.1"
                     /translation="LDNEPALWSSTHPMIHPSQPTCAEVLDKGVSLAAVITSMDPNAQ
                     VLGPVCYGWAEYVNNQSAPDGSLLTPYNNGNGIQYLNYYLAGMKTASTSAGRRLLDFL
                     DLHWYPEATGGGVRITNDDTSQAVAVARMQAPRSLWDPTYVETSWITSSLGNKPITLI
                     PRLQAAVSQYYPGTKLSFSEYEYGSGEDVSGGVAEADALGILGKYGVYATRWDDGTND
                     NYVKAAYRMYLDYDGTGDKFGTTSISAATSSVSLTSAYAATDSSHPERLNVILLNKDY
                     SNPNTASVTLSHLTGGEQILSIRAFRFDSTSYSLYTPASPPAFTANTFTDTLPFRSAT
                     LYEITLNLATATPTPSG"
BASE COUNT          154 a          284 c          415 g          200 t
ORIGIN      
        1 gcccgagggc gtcggcgtgg ccgtggccag gttcagggtg atctcatata aggtggcgga
       61 ccggaagggc agggtgtcgg tgaaggtgtt ggccgtgaag gcggggggcg aggccggcgt
      121 gtagaggctg taactggtgg aatcgaagcg gaaggcccgg atggacagga tctgctcgcc
      181 gcccgtcagg tggctcaggg tgacgctggc cgtgttgggg ttgctgtagt ccttgttgag
      241 caggatcacg ttgaggcgtt ccgggtggga actgtcggtg gccgcgtagg ccgaggtcaa
      301 cgataccgag ctggtggcgg ccgagatgga ggtggtgccg aacttgtcgc cggtcccgtc
      361 gtaatccagg tacatccggt aggcggcctt gacgtagttg tcgttggtgc cgtcgtccca
      421 ccgggtggcg tagaccccgt acttgcccag gatgcccagg gcgtcggcct cggccacgcc
      481 gccggacacg tcctcgcccg acccatattc gtactccgag aaggacagtt tggtgccggg
      541 ataatactgg ctgacggcgg cctgcagccg ggggatgagg gtgatgggtt tgttgcccag
      601 cgaggaggtg atccagctgg tctccacgta ggtcgggtcc cacagggacc ggggcgcctg
      661 catgcgggcc acggccacgg cctgggaggt gtcgtcgttg gtgatgcgca cccctcctcc
      721 ggtggcctcc ggataccagt gcaggtccag gaagtcgagc aggcgccggc ccgccgaggt
      781 ggaggcggtc ttcatgcccg ccaggtagta gttgaggtat tggatcccgt tgccgttgtt
      841 gtagggggtc agcaggctcc cgtcgggggc gctctggttg ttcacgtact cggcccagcc
      901 gtagcagacc gggcccagga cctgggcgtt ggggtccatg gaagtgatga cggcggccag
      961 gctcacgccc ttgtccagca cctcggcgca ggtgggttgg gaaggatgga tcatggggtg
     1021 ggtcgagctc cagagcgcgg gttcgttgtc cag
//
LOCUS       DASTJJ010000005         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_856603, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000005 DASTJJ010000000
VERSION     DASTJJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856603"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR02_00045"
     CDS             <1..258
                     /locus_tag="VFR02_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868876.1"
                     /translation="PQDKPVTYHLSVADGRSFVYQTSGVLTPKKVTNVSVPLADITAA
                     RLDPSAIRSLSLAVEVRGNTLPVEVYWDYLRLEQDLAPAKK"
     gene            complement(517..1959)
                     /locus_tag="VFR02_00050"
     CDS             complement(517..1959)
                     /locus_tag="VFR02_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868877.1"
                     /translation="MIPPQLGGTKSARAAFGLAFLLLAAAPLAASEDRVKAMGGLGLL
                     SDDETAGLIPFLDGNPAGLGLLPSRDRLDLSGRWDASTDGTRPQSLSTLPVDTGKVIG
                     YQGLMLFPSPQWAFQLAVDGTSLDSQPAFSSMDAYQRSGSFLLGRASYRWGALALGLE
                     TQRTQDSLSFSPGLFDGGVTLGGGQSAQTQWIAKAGLLVDLGEAGGPRWQVGGSFAAP
                     LEPPRQDQTLTLRLPSQPAFTDRRLFTQDGGTVSGGIYYEEPRALQARLLVVVDGLRT
                     RLAQTVSDPSPQFPNEALDPLADQRSVTLSGAAKARIHFNDLDLRLGGAVTAAWETQE
                     RFAPGGGPPSGTDRQTLLQADLEAGLEAPEDYLVGLRLGCSLDRGTIPSTGPTGQPGP
                     VQDCFQAALGGEKWISPEWAARIGVLNSLGFDPGGGQSLTSAVVGGVGFRQQDLQLDG
                     RLTLGETFPLDHSGPTSAQTGFALAGVLFL"
     gene            2189..>2473
                     /locus_tag="VFR02_00055"
     CDS             2189..>2473
                     /locus_tag="VFR02_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET9868878.1"
                     /translation="MKTHTPKPVQTGDIIEVEIQSLAFGGDSVARYEGFALFVRGGLP
                     GERVKVKITQVKDHYATGEIVEIARFSPDRVAPPCPIFEECGGCQWQHLNY"
BASE COUNT          459 a          796 c          804 g          414 t
ORIGIN      
        1 ccccaggaca aacccgtgac ctatcatttg tccgtggccg acgggaggtc cttcgtctac
       61 cagacctccg gggtcctgac ccccaagaaa gtgaccaatg tttccgtgcc cctggccgac
      121 atcaccgccg cccggttgga tccctcggcc atccgttcct tgagcctggc cgtggaggtc
      181 cggggcaaca ccctgccggt ggaggtctat tgggactacc tgcgtctcga gcaggacttg
      241 gcgcccgcca agaagtgacc cggggttgtc ccggggcccg tcgttcttga tccaccgatc
      301 ccttatttac ttagaacttc taacaaaacc tatttcacca cacacgccca agggcggccc
      361 cgctccaaaa attcaatgat ggggcagaca ccaagaacac caaggactgc aaaggctgaa
      421 aagtaggaaa atccgaatta atttgatcgc ttttcttggt gaacttggtg ccttggtggt
      481 gaaaattgcc tttattttga tttttgttag agactcttaa agaaagagca cgcccgccag
      541 ggcgaagcct gtttgggcgc tggtgggccc cgagtggtcg agcgggaagg tctcgcccaa
      601 ggtcaaccgt ccgtccaatt ggagatcctg ctgccggaag cccaccccgc ccaccactgc
      661 ggaggtcagg gactggccgc cccccgggtc gaaccccagg ctgttcaaca ccccgatgcg
      721 cgccgcccat tcgggcgaga tccatttttc gccgcccaag gccgcctgga agcagtcctg
      781 gacgggccct ggttgccccg tggggccggt cgaggggatc gtcccccggt ccagcgaaca
      841 gcccagccga agccccacca ggtaatcctc cggagcctcc aatcccgcct ccaggtcggc
      901 ttgcagcagc gtctgccggt cggttcctga gggcggcccg ccccccgggg cgaagcgctc
      961 ttgggtttcc caggccgccg tcaccgcgcc gcccaaccgc aggtccaagt cgttgaaatg
     1021 gatccgggcc ttggccgcgc ccgacaaggt gacggacctt tggtcggcca gcgggtccag
     1081 ggcctcattg gggaattggg gcgaggggtc ggagacggtt tgggccaggc gggtcctgag
     1141 gccgtccaca acgaccaaaa ggcgggcctg gagcgcgcgg ggctcctcat aatagatacc
     1201 gccgctgacg gtcccgccgt cctgggtgaa aagccgccgg tcggtgaagg ccggttggct
     1261 ggggagccgc agggtcaggg tctggtcctg ccggggcggc tccaaagggg cggcgaagga
     1321 cccgccgacc tgccaccggg ggcctcccgc ctccccgagg tcgaccagca ggcccgcctt
     1381 tgcgatccac tgggtttggg cgctttggcc cccccccagg gtcaccccgc catcaaaaag
     1441 tcccggagaa aaagaaaggc tgtcctgggt cctttgggtt tccaggccca aggccagcgc
     1501 gccccaccga taagaggccc ggcccagcaa aaaggaaccg gaccgttgat aggcatccat
     1561 cgaggaaaag gcgggttggg aatcgaggct tgtcccatcg accgccaatt ggaaggccca
     1621 ttggggggaa gggaatagca tgaggccttg gtaaccgata accttaccgg tgtccacggg
     1681 caatgtgctg aggctttggg gccgggtgcc gtcggtggag gcgtcccagc ggccggatag
     1741 gtccaagcgg tcccgggaag gcaagaggcc caggccggcg gggttcccgt cgaggaaagg
     1801 gattaacccg gcggtttcgt cgtccgaaag caggccgagc ccgcccatgg ccttcacccg
     1861 gtcctcggac gccgccaggg gcgcggcggc cagcagcagg aaggccagcc cgaaggccgc
     1921 ccgggccgac ttggttccgc cgagctgggg agggatcacg ggcggagtat atcagaggcg
     1981 atgcgggttg ccgacgcctg aaaatcggtt aaaataaggg aaaggccccg gtggtgacgt
     2041 ttccgaaggg acccttccag gcgcgatcat tttggactgc ctcccaagat ccgcgcgaga
     2101 cccaattgag aatccagcgt gaagctcccc tcccgcgcgg acccgtccgc gtccggggtt
     2161 ttgcggcttg agccgctcga aggaacgcat gaagacccat acgccgaagc ccgtgcaaac
     2221 cggggacatc attgaagtgg agatccagtc gctggccttc gggggcgact cggtggcccg
     2281 ttacgagggt ttcgccctct tcgtccgcgg gggactgccc ggcgaacggg tcaaggtcaa
     2341 gatcacccag gtcaaggacc attacgccac cggcgagatc gtggagatcg cgcgcttttc
     2401 ccccgaccgg gtggccccgc cctgtcccat cttcgaggaa tgcggcggct gccagtggca
     2461 gcacctgaac tac
//
LOCUS       DASTJJ010000006         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_367613, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000006 DASTJJ010000000
VERSION     DASTJJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367613"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="fabD"
                     /locus_tag="VFR02_00060"
     CDS             complement(<1..830)
                     /gene="fabD"
                     /locus_tag="VFR02_00060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9868879.1"
                     /translation="MAKRAFVFPGQGSQSVGMGKDLAEAFPEARALFDRADAALGFPL
                     SKLCFEGPEEELKKTANTQPALLAVSLAAFGLLKGKGVGFDAVAGHSLGAYSALTAAG
                     VMGFEDAIRLVRRRGELMEQAGAGKGTMAVILNLDEDKVAQACAEASSLGVVEPANVN
                     CPGQIVISGEKAAVAAACEKAKALGAKRAMELPVSGPFHSSLMKQAAEGLRQALASVK
                     LAAPQAAYYSDVDAKPLADPGEIAESLVRQLVAPVQWTRTIEALGRDGVGAFVEVGSG
                     "
     gene            complement(833..>1051)
                     /locus_tag="VFR02_00065"
     CDS             complement(833..>1051)
                     /locus_tag="VFR02_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET9868880.1"
                     /translation="ANLRIIQAIGERMEVPASKMVVNLDRVGNTSAASVIIALDECVR
                     AGRLKPGDILVLVAFGAGTTLASSVVRW"
BASE COUNT          161 a          395 c          333 g          162 t
ORIGIN      
        1 ttgcccgacc ccacttccac gaaggccccc acgccgtcgc ggcccaaggc ctcgatggtc
       61 cgggtccatt gcacgggcgc caccaactgg cgcaccaggc tctcggcgat ctcccccggg
      121 tcggccaggg gcttggcgtc gacgtccgaa taataggccg cctggggcgc ggcgagcttc
      181 accgaagcca gggcctgccg cagcccctcg gcggcctgct tcatcagcga gctgtggaag
      241 ggtccgctga ccggcagttc catggcgcgc ttggcgccca gggccttggc tttctcgcag
      301 gccgccgcca cggccgcctt ctcgcccgag atgacgatct ggccagggca gttcacgttg
      361 gccggttcca ccacgcccag cgaggaggcc tcggcgcagg cctgggccac cttgtcctcg
      421 tccaagttca ggatgacggc catggtgccc ttgcccgccc ccgcctgttc catcaattcc
      481 ccgcggcggc ggaccagccg gatcgcgtcc tcgaacccca tcaccccggc ggccgtcagc
      541 gcgctgtagg cgcccaggct gtggcccgcc acggcgtcaa aacccacgcc cttgcccttg
      601 agcaacccga aggccgccag ggacaccgcc agcagggcgg gctgggtgtt cgcggtcttc
      661 ttcagttctt cttccgggcc ctcgaaacaa agcttggaca gggggaaccc gagggccgcg
      721 tcggcccgat cgaacaatgc ccgggcctcg gggaaggcct ccgccaggtc cttgcccatg
      781 cccacggact gggaaccctg gccgggaaat acgaaggcgc gtttggccat gcctaccacc
      841 tcaccacgga agatgccagc gtggtccccg ccccgaaggc caccaggacc aggatgtcgc
      901 ccggtttcaa acggcccgcc cggacgcatt cgtccagcgc gatgatgacg gaggccgcgg
      961 acgtattgcc cacccggtcc aggttgacca ccatcttgga cgcgggcact tccatgcgtt
     1021 ccccgatggc ctggatgatg cgcaggttgg c
//
LOCUS       DASTJJ010000007         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1591036, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000007 DASTJJ010000000
VERSION     DASTJJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591036"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1747
                     /locus_tag="VFR02_00070"
     CDS             <1..>1747
                     /locus_tag="VFR02_00070"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HET9868881.1"
                     /translation="SGSPNSADTGYAGYLVNYIKYVNSQLAGNGISVLAVSPQNEPDW
                     NPTYESALWTAGQFDVFAGALHSSLQSAGLSTRIMIPESFRDDKNLAATAMDDAAIAP
                     FIGVIGNHLYGLNGAQPYSLAGAGFTHLTNQENWETEMSDVSGAANDTSMNSGFQEAG
                     WIQQCIVGASMNAYHAWWLYPTGNTNEALIGTDNQSTKKLWVVGNWSRFVRPGYYRMG
                     ATVSPSSGVSLSAFKSDSSSAPASFVIVAINRNNSATSTTFNLNGINATSVTPWVTDA
                     SNNLTRQTAVAVSGNSFTYNLNAQSVISFVGAVTGGGGPTATPTLTPVPLSPTPSPTP
                     LTASTWRVNAGGPAYTDSLGNVWSADTQFTAGTTIAEGGTIAGTSDSTLYDTQRYGSN
                     FSYVFHVPAGTYQATLYLAETYSGDASVGARVFSVLVNGVTMVSNLDLYAQGGANQAL
                     NQVLNNLTPSAGAVTLQFVSTGGTDANALVEAIQVIPQPNLATPTRTTTATVTATRTP
                     LPPSPTATWTASSTATATATNSPLPATFTPTATASPTPLTASTWRVNAGGPAYTDSLG
                     NAWQADAQFVGGTTIA"
BASE COUNT          306 a          693 c          493 g          255 t
ORIGIN      
        1 gctcgggttc ccccaactcc gccgacacgg gctacgcggg ctacctggtc aactacatca
       61 agtacgtcaa ctcgcagctc gccggcaacg ggatcagcgt cctggccgtg tccccccaga
      121 acgagccgga ttggaacccc acctatgagt ccgccctctg gaccgccggc cagttcgacg
      181 tcttcgccgg ggccctccat tcctccctcc aaagcgcggg cctttccacc aggatcatga
      241 tccccgagag cttccgggac gacaagaacc tggcggccac cgccatggac gacgccgcca
      301 tcgcgccctt tatcggcgtc atcggcaacc acctctacgg cctgaacggg gcccaaccct
      361 attcgctggc cggggcgggc ttcacccacc tgaccaacca ggagaactgg gagaccgaga
      421 tgtcggacgt cagcggggcg gccaacgaca cctccatgaa ctcgggtttc caggaggcgg
      481 gatggatcca acagtgcatc gtgggcgcca gcatgaacgc ctatcacgcc tggtggctct
      541 acccgacggg gaacacgaac gaggccctga tcggcacgga caaccaatcc accaagaagc
      601 tctgggtggt gggcaactgg agccgcttcg tgcggcccgg ctactaccgc atgggcgcca
      661 cggtctcccc ctcgtcgggc gtctcgctgt cggccttcaa gagcgattcc agctcggccc
      721 cggcctcctt cgtcatcgtg gccatcaacc gcaataattc ggccacctcc accaccttca
      781 acctcaacgg catcaacgcc acctcggtga cgccctgggt gaccgacgcc agcaacaacc
      841 tgacccggca gacggcggtg gccgtcagcg gcaattcgtt cacctacaac ctcaacgcgc
      901 agagcgtcat ttccttcgtg ggcgcggtga cgggcggcgg cggccccacg gccaccccga
      961 ccctgacgcc ggttcccctg tcccccacgc ccagcccaac gcccttgacg gcctccactt
     1021 ggcgcgtgaa cgcgggcggc ccggcctata cggactcgtt gggcaacgtt tggtcggcgg
     1081 acacccagtt caccgccggc accaccatcg ccgagggcgg gaccatcgcg ggcacctccg
     1141 actcgaccct ctatgacacc cagcgctacg gctccaactt cagctacgtc ttccacgtcc
     1201 ccgcggggac ctaccaggcc accctgtact tggccgagac ctattcgggc gacgcctccg
     1261 tgggcgcccg cgtcttcagc gtcttggtga acggggtgac gatggtgtcc aacctcgacc
     1321 tctacgccca aggaggggcc aaccaggccc tgaaccaagt gctcaacaac ctgacgccca
     1381 gcgcgggagc ggtcacgctg cagttcgtgt ccacgggcgg gacggacgcc aacgccctgg
     1441 tggaggccat ccaggtcatc ccccagccca acctcgcgac ccccacccgc acgaccaccg
     1501 ccaccgtgac cgcgacccgc acgcccctgc ctccgtcccc cacggccacc tggacggcgt
     1561 cctcgaccgc gaccgccacg gccacgaatt cacccctgcc ggccaccttc acccccaccg
     1621 cgaccgccag ccccacgccc ttgacggcct ccacctggcg ggtgaacgcg ggcggcccgg
     1681 cctatacgga ttcccttggt aacgcctggc aggcggacgc ccagttcgtg ggcggcacca
     1741 ccatcgc
//
LOCUS       DASTJJ010000008         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_979825, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000008 DASTJJ010000000
VERSION     DASTJJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979825"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFR02_00075"
     CDS             complement(<1..383)
                     /locus_tag="VFR02_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9868882.1"
                     /translation="MTAALPPILNGNFFARALSHLWGAFFAAPARDPGREADRRLVEE
                     ALSGDPGFERLVRAHEAMVYRTAFRFLGQEADALDVAQEVFLRVHRSLAKFRGDSALS
                     TWIYAITANLSKNALRSRKNRVKLQV"
     gene            complement(405..>1123)
                     /locus_tag="VFR02_00080"
     CDS             complement(405..>1123)
                     /locus_tag="VFR02_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9868883.1"
                     /translation="RLIGEDPDSDLAVIRVDVQGLTPAPLGVAGALRVGQLVVAIGSP
                     LGFQTTVTAGVVSALGRSLRSQSGRLIDNIIQTDAALNPGNSGGPLVNAWGQVVGVNT
                     ATIRSAQGLCFAIAVETAQRVAMQLMAHGRVRRSFIGIGGQDIDLPRRAVRHFGLRQE
                     KGVLLVAVEPSGPAARAGLEERDVLVELEGQALSGMDDLHRLLGEERVGVPTALGVLR
                     RSERLDLKVIPVEARPVGPA"
BASE COUNT          170 a          405 c          394 g          154 t
ORIGIN      
        1 acctgcagct tgacccggtt cttgcgcgag cgaagcgcgt tcttcgacag gttggccgtg
       61 atggcataga tccaggtgga cagggcgctg tcgccccgga acttggccaa ggaccggtgg
      121 accctcaaga aaacctcctg ggccacgtcc agggcgtcgg cttcctgccc caggaagcgg
      181 aaggctgtgc ggtagaccat ggcctcgtgg gcccgcacca gccgctcgaa ccccgggtcg
      241 cccgacaggg cctcttccac caggcgccgg tccgcttccc ggccggggtc ccgggcgggg
      301 gcggcgaaga acgcgcccca caggtgggac agtgcccggg cgaaaaagtt cccgttcaag
      361 atggggggaa gggcggcggt catggcgcca gtttagcacg ttttttaggc tgggccgacg
      421 ggacgggcct ccaccgggat gaccttcagg tccaaccgct ccgagcggcg caggacgccc
      481 aaggcggtgg gcacgcccac ccgctcctcg cccagcagcc ggtgcaggtc gtccatgccc
      541 gacagggcct ggccttccag ttccaccagc acgtcccgtt cctccaggcc ggcccgggcc
      601 gccggacccg agggctcgac ggccaccagc agcacgcctt tttcctgccg caggccgaaa
      661 tggcgcaccg cccggcgggg caggtcgatg tcctggcccc cgatgccgat gaagctgcgc
      721 cgcacccggc cgtgggccat gagctgcatg gccacccgtt gggccgtttc caccgcgatg
      781 gcgaagcaca ggccctgggc cgagcggatg gtggcggtgt tgacgcccac cacctgcccc
      841 caggcgttca ccaggggtcc gcccgaattg ccggggttca gggccgcgtc ggtctggatg
      901 atgttgtcga tgagccgccc cgactgggac cgaagcgagc gccccagggc ggacaccacc
      961 ccggccgtca cggtggtctg gaaacccagg gggctgccga tggccaccac cagttggccc
     1021 acccgcaggg cgccggccac gcccaggggg gccggggtca gcccctgtac gtccacccgg
     1081 atgaccgcca ggtccgaatc cgggtcctcg ccgatgaggc ggg
//
LOCUS       DASTJJ010000009         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1591406, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000009 DASTJJ010000000
VERSION     DASTJJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591406"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..>1317)
                     /gene="gcvPB"
                     /locus_tag="VFR02_00085"
     CDS             complement(12..>1317)
                     /gene="gcvPB"
                     /locus_tag="VFR02_00085"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9868884.1"
                     /translation="AEIELVRYYTALARKNFCIDNGFYPLGSCTMKYNPKVHEDIAFR
                     DEWADLHPAQREDEVQGALQVMYDLEHWLVEVTGCHALTLQPAAGAQGELTALLMAKR
                     YFADRGEHQRVNVIVPDSAHGTNPASATMAGWKTVSIPTDARGNMDLAALKAKLGRDT
                     AVLMLTNPSTLGLFDEHILEVAKLVHDAGGLLYYDGANMNAILGICRPADMGFDLVHL
                     NLHKSFSTPHGGGGPGSGPVGCTKALEPYLPEPRVAREGNRYFLRSGSPQSIGRMKGN
                     VGNFGVLLRALAYCKRNGKEGLERVGRVATLNANYLLAKLKGAYPVAYDRLCKHEFVL
                     TAEPLKKKYGVSALDIAKRLIDFNYHPPTIYFPLVVPESIMIEPTETETKRTLDEFVD
                     AMLKIRREMETNPDIFKNAPYSTEVSRLDEVKAVKEPKLVKK"
BASE COUNT          213 a          433 c          416 g          255 t
ORIGIN      
        1 ggccttgagg gctacttctt caccagcttg ggttccttga ccgccttgac ctcgtccagc
       61 cgggaaacct cggtgctgta gggtgcgttc ttgaagatgt ccgggttggt ctccatttcc
      121 cggcggatct tgagcatggc gtccacgaac tcatccaggg tccgcttggt ctcggtctcg
      181 gtgggctcga tcatgatgga ctcgggcacc accagcggga aatagatggt gggcgggtgg
      241 taattgaagt cgatgagccg cttggcgatg tcgagcgcgg acacgccgta ctttttcttc
      301 aagggttcgg ccgtcagcac gaactcgtgc ttgcacagcc ggtcataggc caccggatag
      361 gcccccttga gcttggccaa gaggtaattg gcgttgaggg tcgcgacgcg gcccacccgc
      421 tccagccctt ccttgccgtt gcgcttgcag taggccagcg cccgcagcag cacgccgaag
      481 ttgcccacgt tgcccttcat gcgcccgatg gactgggggg agccggacct caggaaatac
      541 cggttcccct cccgcgccac ccggggctcg ggcaggtagg gctccagcgc cttcgtgcag
      601 cccaccggcc ccgagcccgg ccccccgccg ccgtggggcg tcgagaaact tttatgcagg
      661 ttcagatgca ccaagtcgaa ccccatatcc gccggtcggc agatccccaa aatggcgttc
      721 atattggcgc cgtcgtaata cagaagaccc cccgcgtcat gcaccagctt ggccacttcc
      781 aaaatatgct cgtcgaacaa cccgagcgtg ctcgggttcg tcaacatcaa gaccgccgtg
      841 tcgcgcccca gcttggcctt cagggccgcc aggtccatgt tgccccgcgc gtcggtgggg
      901 atggagaccg tcttccagcc cgccatggtg gccgaggccg ggttggtgcc gtgggcggag
      961 tcgggcacga tcacgttgac cctttggtgc tcgccccggt cggcgaaata acgcttggcc
     1021 atcaagagcg ccgtcagctc cccctgggcc cccgcggcgg gctgcagggt cagggcgtgg
     1081 cagcccgtga cctccaccag ccaatgttcc aggtcgtaca tcacctgcag cgccccctgc
     1141 acctcgtcct cgcgttgggc gggatgcagg tcggcccact cgtcgcggaa ggcgatgtcc
     1201 tcgtgcacct tggggttgta cttcatggtg caggagccca acggatagaa accgttgtcg
     1261 atgcagaaat tcttgcgggc cagggccgtg tagtagcgca ccagctcgat ctcggcg
//
LOCUS       DASTJJ010000010         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_613382, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000010 DASTJJ010000000
VERSION     DASTJJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613382"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..463)
                     /locus_tag="VFR02_00090"
     CDS             complement(53..463)
                     /locus_tag="VFR02_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868885.1"
                     /translation="MDEVQKPMTEAEKQIRRLEALRAKREEDSKQSAEKAFLPPRTGV
                     ERTIEKLKAMKARSASPAGSKRAKIEGRQKNAKLSNLQLTVLEQLLARDKRLRGSRII
                     RIALSRLLELDLSPEEVELETRIGELLRQMKSRP"
     gene            complement(429..1364)
                     /locus_tag="VFR02_00095"
     CDS             complement(429..1364)
                     /locus_tag="VFR02_00095"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9868886.1"
                     /translation="MRGKGTGAGGRLCVGPVDSLVLCYQVVLSGVVLFSRIPPGEKAR
                     LVGEHVCLLAAIPLFLLWARDHAGPVVRFVRDLYPLLLRLFFYKEIGLLIHGYFDWSL
                     DSWLLSKDYQLGHIALSVWHLQQFQPPPRFLNELFSLGYGFYLVLIPLAALTLYVRAK
                     PEVFRTFLFTISFTYYLCYLMFIFLPAESPRFYIPGLRESLQGYWASRWVHWAVEGNA
                     FPGGSFPSSHIAASVICLRCFPYLGRWRYLAGFFTLLLFLGTIYGRYHYFIDVTAGLV
                     VGLVCDRLAPWLEKRWPLVWNWSPHGRSPKANDRS"
     gene            complement(1361..>2244)
                     /locus_tag="VFR02_00100"
     CDS             complement(1361..>2244)
                     /locus_tag="VFR02_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868887.1"
                     /translation="YWSLGDAPDLSPQASAYDYVNDFREFFNALKQEDPSLIVMGPNL
                     AFPDGRSGTEWMGAFLRYDGDIVNLASVQHYACGAVSQLSPGGLWDDVRGLTPAYRAL
                     QGRVCQDSDLYLPVVYTRMGLYPGTDVPEDFTQTLWRAEVLGTLLQEGAEMMVAGDLE
                     GPGKTGLFAQGVPDPAARVLELFSAVWKGIPVPAKAKKNGIDVFAAQDPVTRDLSLFI
                     VNRNTRYEWMDVSLDGHGDNLVVEAGLSRRFRLEVPDQALVAVRAPADGSPVTVEVYG
                     AAQAKKGGGPLSETLRP"
BASE COUNT          478 a          709 c          701 g          356 t
ORIGIN      
        1 agtcaatcac ctcggaattt cgataaacgg ggcggcaagg aaggcgggcg gatcaggggc
       61 gggacttcat ctgccggagc agctccccga tccgggtctc cagctccact tcctcgggcg
      121 aaaggtccag ttccaacagg cggctgaggg cgatgcggat gatccggctt ccgcgcagac
      181 gcttgtccct ggccaagagc tgctccaaca cggtcagctg caggttggag agtttggcgt
      241 ttttttgacg gccctcgatc ttggcgcgct tgcttcccgc cggggacgcg gaccgggcct
      301 tcatggcctt gagtttctcg atggtccgct ccaccccggt gcggggggga aggaaagcct
      361 tctcggcgga ctgtttggag tcctcttcgc gtttggcgcg cagggcttcc agtcggcgga
      421 tctgcttttc agcttcggtc attggctttt ggacttcgtc catggggact ccagttccag
      481 accagtggcc agcgcttttc cagccagggc gccagccggt cacagactaa acctactacc
      541 aaacccgccg tcacatcaat gaaataatga taacgcccgt aaatggtccc caagaagagg
      601 agcagggtga aaaaaccggc caaatagcgc cagcgtccca gatagggaaa acagcgaagg
      661 cagatgaccg aggccgcgat gtgcgaactg ggaaaggaac ctcccgggaa ggcgttgcct
      721 tccacggccc aatgcaccca ccgggaggcc cagtagccct gcagggattc ccgaaggccc
      781 gggatgtaga aacgcgggct ctcggcgggc aggaagatga acatcaggta gcaaagataa
      841 taagtgaagc tgatcgtgaa gaggaaggtg cggaagacct ccggcttggc ccgcacatag
      901 agcgtcaagg ccgccagcgg gatcaggacc aaataaaaac catagcccag gctgaacaat
      961 tcgttcagga accgtggggg tggctggaac tgctggagat gccacacgga cagggcgata
     1021 tgccccagtt ggtagtcctt gcttaggagc cagctgtcca ggctccagtc gaaatagccg
     1081 tggatcagga gcccgatctc cttataaaag aacaacctga gcaggagcgg atagaggtcc
     1141 cgcacgaacc ggaccaccgg accggcgtgg tcccgcgccc acaggaggaa cagcgggatg
     1201 gccgccagca ggcagacgtg ttcccccacc aagcgcgcct tttcacccgg ggggatcctg
     1261 gaaaaaagca ccacgccgga caggaccact tggtagcaga gcaccagcga atccaccggg
     1321 cccacgcaaa gccgcccccc cgccccggtc cccttccccc tcatggacgc aacgtctctg
     1381 aaagcggccc gccacccttt ttcgcctggg cggccccata gacctcaacg gtcaccggcg
     1441 atccatcggc gggggcgcgg accgccacca gggcctggtc gggcacctcc aggcggaaac
     1501 ggcgggagag gcccgcctcc accaccaggt tgtcgccgtg gccgtccaag gaaacatcca
     1561 tccactcata acgggtattc cgattcacga tgaagagcga aaggtcccgg gtcacgggat
     1621 cctgggcggc gaagacgtcg atcccgttct ttttcgcttt ggccggcacc gggatcccct
     1681 tccagaccgc ggagaaaagc tccaggaccc gggccgcggg atcggggacc ccctgggcaa
     1741 agaggcccgt ttttccaggc ccctccaggt cgcccgccac catcatctcg gcgccttcct
     1801 gcaacagggt cccgaggacc tcggcccgcc aaagggtctg ggtgaagtct tccgggacat
     1861 ccgtcccggg ataaagaccc atccgggtat agaccaccgg aaggtaaagg tccgaatcct
     1921 ggcagacccg gccctgcagc gcccggtacg ccggcgtgag gccccggaca tcgtcccaaa
     1981 gcccgcctgg ggaaagctgg gaaaccgcgc cacaggcgta atgctggacc gaagccaggt
     2041 tcacgatgtc cccgtcgtac cggaggaagg cgcccatcca ttccgtcccc gaccggccgt
     2101 cgggaaaggc caggttgggg cccatcacga tcagggaagg gtcttcttgc ttcaaggcgt
     2161 tgaagaattc acggaaatca ttgacgtagt cataggccga ggcctggggg gagaggtccg
     2221 gggcatcccc cagggaccaa tagc
//
LOCUS       DASTJJ010000011         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_613563, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000011 DASTJJ010000000
VERSION     DASTJJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613563"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFR02_00105"
     CDS             <1..728
                     /locus_tag="VFR02_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9868888.1"
                     /translation="LHVGLFTRPSRLRQFVRLALAEKPDLIAIAGDITDDNAYYIPKF
                     LRSLKAVEAHIPMVAILGNHDLYGGGHETIAELRGSPLRILVNEGFALRRGKAALWVA
                     GLSDDAARRHQRFNDLLPDLGLCLKGRPKGAPTVLLAHQPHAFEEAAKRGVELTLSGH
                     THGGQLGIRFLDWSLAKVFTPWHMGHFQRGKSRLYVTVGTGFWMVPFRFGLPPEISIL
                     ELRQNTKKQDPSSPHTPKGGPVP"
     gene            complement(852..>2097)
                     /locus_tag="VFR02_00110"
     CDS             complement(852..>2097)
                     /locus_tag="VFR02_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9868889.1"
                     /translation="HKTDPGSFDLQEVIESGLIETHFQPLVSLKRKRVLGYEALSRGI
                     HPSDQSVIPPTLLFTEASAQGLQLELDRLCRKKAMEAFRVLPGAGEAILSLNLDASVM
                     DQGAAGSGHLRNQVMEMGLNPRNIAIEIIESNVEDMKELQKFVQTYRGYGFLIALDDV
                     GAGHSNLNRIPLIKPDILKIDRYLIQDIQDDFYKQEVLKSLLSMARFLGTLIIAEGVE
                     TEEEAMTLLDMGVDMVQGYFFARPQPPARLDNAAVLDKVERLGAAFKQKLMRKIDRKR
                     FNMDKYYSMILEMQIDLEKCGEGQFDGRLSEMITKFPQAEGLYILDEAGIQVTDTVFH
                     PRAAGRRNPVMFHPTARGADHTLKDYYYFLMEADLGKASFVTKPYLSMASGNVCVTLS
                     ALFKDSTQKKYILCIDINTEGL"
BASE COUNT          369 a          649 c          671 g          408 t
ORIGIN      
        1 acctgcacgt gggcctcttc acccggccct cgcgcctgag gcagttcgtg cggctcgccc
       61 tggcggagaa gccggacctc atcgccatcg cgggcgacat cacggacgac aacgcctatt
      121 acatcccgaa gttcctccgc agcctgaagg ccgtcgaggc ccatatcccc atggtggcca
      181 tcctgggcaa ccacgacctc tacggcgggg ggcatgagac catcgcggaa ctgaggggca
      241 gcccgctgcg catcctggtc aacgagggct tcgcgttgcg gcggggcaag gcggcgcttt
      301 gggtggcggg gctttcggac gacgcggccc ggcgccacca gcggttcaac gacctgctgc
      361 ccgacctggg cctctgcctg aagggccggc ccaagggcgc gcccaccgtc ctgttggccc
      421 accaacccca cgccttcgag gaagccgcca agcggggcgt ggagctgacc ctgagcggcc
      481 atacccacgg ggggcagttg ggcatccggt tcctggattg gtcgctggca aaggtcttca
      541 cgccctggca catggggcat ttccagcggg ggaaaagccg gctttacgtg acggtgggga
      601 cgggcttctg gatggtgccc ttcaggttcg gcctgccgcc cgagatcagc atcttggaac
      661 tcagacaaaa cacaaaaaaa caagatcctt cttcaccaca cacgcccaag ggcggccccg
      721 ttccataaaa ttaatgttgg ggcaggcacc aagacaccaa gaaaatcaaa acttagatct
      781 ttagatttag ccttccttgg agtcttggtg tcttggtggt gaaacgatct tggagtcggc
      841 cgtttctttg attataggcc ttcggtgttg atgtcgatgc agaggatgta tttcttctgc
      901 gtcgaatcct tgaagagggc cgagagggtc acgcagacgt tgcccgaggc catggacagg
      961 tagggtttgg tcacgaagct ggccttgccc aggtcggcct ccatgaggaa gtagtaatag
     1021 tccttcagcg tatggtccgc gccccgggcg gtggggtgga acatgaccgg attgcggcgc
     1081 ccggccgcgc gcgggtggaa gaccgtgtcg gtgacctgga tccccgcctc gtccaggatg
     1141 tagagcccct cggcctgggg gaacttggtg atcatctcgg acaggcgacc gtcgaactgg
     1201 ccctccccgc acttttccag gtcgatctgc atctccagga tcatggaata gtacttgtcc
     1261 atgttgaagc gcttgcggtc gatcttgcgc atcagcttct gcttgaaggc cgcccccaac
     1321 cgctccacct tgtccaacac cgccgcgttg tccaaccgcg cgggcggctg gggccgggcg
     1381 aagaaatacc cctgcaccat gtccacgccc atgtcgagca gggtcatggc ttcctcctcg
     1441 gtctccacgc cctcggcgat gatgagcgtc cccaggaacc gggccatgga caggagcgac
     1501 ttgagcacct cctgcttgta gaaatcgtcc tggatgtcct ggatcaggta gcggtcgatc
     1561 ttgaggatgt cgggcttgat gagggggatg cggttcaggt tcgaatggcc cgcgcccacg
     1621 tcgtccagcg cgatgaggaa cccgtagccc cggtaggtct gcacgaactt ctgcagttcc
     1681 ttcatgtcct cgacgttgga ctcgatgatc tcgatggcga tgttgcgggg gttcaggccc
     1741 atctccatca cctggttgcg caggtggccc gaccccgccg cgccctggtc catgaccgag
     1801 gcgtccaggt tcagggacag gatggcctcg cccgccccgg gcaggacccg gaaggcctcc
     1861 atggccttct tgcggcacag gcggtccagt tccagctgca ggccctgcgc gcttgcctcg
     1921 gtgaaaagca gggtgggcgg gatgaccgat tggtccgagg ggtggatgcc ccggctgagc
     1981 gcctcataac ccaacacgcg ctttcgcttg agggacacca ggggctggaa atgcgtttcg
     2041 ataaggccgc tttcgatgac ttcctgcagg tcgaatgagc ccgggtcggt cttgtgc
//
LOCUS       DASTJJ010000012         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1103032, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000012 DASTJJ010000000
VERSION     DASTJJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103032"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1056)
                     /locus_tag="VFR02_00115"
     CDS             complement(76..1056)
                     /locus_tag="VFR02_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868890.1"
                     /translation="MAFTRRFLVEPDLESCLSLLREGFAFKAPQRPRLTALWRELLEG
                     GHCITVAVDDRRRPAGGRLVGFGMSVFVTDAFARLLLAGEPFLSRAFLREWEAGRRPY
                     LLKKEVAAANAGDGLNLMVLHYGWREKEMLPEELDRVRLMQTEAFIQQHAGFKVKEYL
                     HEVFGPQLRDFVLSAGSVLRHDYAEPRWKAALKGLGPGDRPYLTGFRPEEVLARAGTA
                     ASLLQAKAAAPRFTLSPGEQDMLAKALQGETDPHLARSLGLSPWTIKKRWQSVYLKVD
                     KADPDLLGGPKAAGRKHTDAQPQRRRYLLEYLRHHPEELRPHAPGRPRRG"
     gene            complement(1116..>1663)
                     /locus_tag="VFR02_00120"
     CDS             complement(1116..>1663)
                     /locus_tag="VFR02_00120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9868891.1"
                     /translation="AASGPGPLSFSFRLRPGVPADIPALSRAYARSWRETYGAFAPAA
                     FTEGMTEAAAARIFAESLGPNAFSYFLYVAEAEGSLVGFADGGRERGRPEGGEGELYA
                     LYLLRDFQGRGMGRALLGAALARLGAQGLHPGVAWVLEHNPSRKFYEAMGGTLGRDRK
                     TLRVGDLPLTLVPYRWATKPL"
BASE COUNT          275 a          556 c          593 g          239 t
ORIGIN      
        1 gatcgaaggt tggattatgg aaaaaagcgg ggagggcgca taggcccctt ttggtgtagg
       61 aaaaagggaa acggattacc cacggcgcgg ccgtccaggg gcgtggggcc gcagttcctc
      121 cggatggtgg cgcaggtatt ccaagagata gcggcgccgc tggggttggg cgtccgtgtg
      181 cttcctgccg gcggccttgg gcccgcccaa aaggtccggg tccgccttgt ccaccttcag
      241 gtagacggac tgccagcgtt tcttgatggt ccagggcgaa agccccagcg agcgggccag
      301 gtgcgggtcc gtctcccctt gcagggcctt ggccagcatg tcctgctcgc cgggggaaag
      361 ggtgaaacgg ggcgccgccg ccttggcctg caggagggac gccgccgtgc ccgcccgggc
      421 cagcacttct tcggggcgga aaccggtcag gtagggccgg tcccccggac ccagcccctt
      481 gagcgccgcc ttccagcggg gttcggcgta atcatggcgc agcacgctgc ccgcggagag
      541 cacgaagtcg cgcagttggg gcccgaagac ctcgtgcagg tattccttga ccttgaaccc
      601 ggcgtgttgc tggatgaagg cctcggtctg catcaagcgc acccggtcca gctcctcggg
      661 caacatctcc ttttcgcgcc agccgtaatg cagcaccatc aggttcaggc cgtcgcccgc
      721 gttggcggcg gcgacctcct tcttcagcag gtagggccgg cggcccgctt cccattcacg
      781 caggaaagcc cgggacagga agggctcccc cgccagcagc agccgggcga aggcgtcggt
      841 gacgaagacc gacatgccga accccaccag gcgcccgccc gcggggcgcc ggcggtcgtc
      901 caccgccacc gtgatgcaat gcccgccttc cagcagctcg cgccaaagcg cggtcagccg
      961 gggcctttgg ggggccttga aggcgaagcc ttcccggagc agggaaaggc agctctccag
     1021 gtcgggttca acgaggaagc ggcgggtgaa ggccatgggg gacgcctggg atcgggatgg
     1081 aagcgggctt attttgtcag gaagcggcgt caaattcaaa ggggtttggt cgcccatcgg
     1141 tagggcacca gggtgagggg caggtccccc acccgcaggg tcttgcggtc gcggcccagg
     1201 gtccccccca tggcctcgta gaatttccgg ctgggattgt gctccagcac ccaggccacc
     1261 cccgggtgga gcccctgggc ccccaggcgg gccagcgccg cccccaaaag ggcccgtccc
     1321 atgccccggc cctgaaaatc gcgcaacagg tagagggcgt agagttcccc ctcgccgccc
     1381 tcggggcggc cgcgttcccg gcccccgtcg gcgaagccca ccagggaccc ctccgcctcg
     1441 gccacgtaga ggaaatagga aaaggcgttg ggccccaggc tttcggcgaa gatgcgggcg
     1501 gccgccgcct cggtcatgcc ttccgtgaag gccgcgggcg cgaaggcgcc ataggtctcc
     1561 cgccaagacc gggcataggc ccgggacagg gccgggatgt cggccgggac gcccggcctg
     1621 aggcggaagg aaaagctcaa agggccaggg cccgaagctg ccc
//
LOCUS       DASTJJ010000013         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_613844, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000013 DASTJJ010000000
VERSION     DASTJJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613844"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFR02_00125"
     CDS             <1..313
                     /locus_tag="VFR02_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9868892.1"
                     /translation="PEAPAGTFALVTVTEVLEHLPDPVATLQAWVDRLEPGGMVAGMT
                     LFHPDDPAKFDAWPYRRDLTHVSFYMPKTLETLAQTLGLSPRFTDGERYFVWEKAGTV
                     G"
     gene            487..999
                     /locus_tag="VFR02_00130"
     CDS             487..999
                     /locus_tag="VFR02_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868893.1"
                     /translation="MRRFCVDHGLDPSNWSKVERGVHPAPKDERTLREWAKYFGLKPH
                     SEGWDRFMDEARISRGEIPKDILKDEEVLSLLPAFFRTVRGVGIGGPEAERLIEKIRE
                     ANRPGNPTPRLQKTVKKIRLGKAADRDDVEYWLSRPAAERIEAVELLRKERYGSSARL
                     QGTFRVIEPA"
     gene            947..1393
                     /locus_tag="VFR02_00135"
     CDS             947..1393
                     /locus_tag="VFR02_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868894.1"
                     /translation="MEAQPDFKELFALLNRHKAEFIIVGAYALAYHGIPRNTGDIDVY
                     VRPTALNARRVLAALGDFGFKALGLTEADFQRPGNIIQLGVPPVRVDFLTALSGVTWK
                     ESDQGKAAGNYGGVKVHYLGRAQLVANKRKTARKKDLADLEALGEG"
BASE COUNT          403 a          558 c          631 g          385 t
ORIGIN      
        1 tcccgaggcc ccggcgggca ccttcgccct ggtgaccgtg accgaggtgt tggaacacct
       61 gcccgacccg gtggcgaccc ttcaggcttg ggtggatcgg ctggagccgg gcgggatggt
      121 cgccggcatg acgctttttc atcccgatga ccccgcgaag ttcgacgcat ggccctaccg
      181 gcgcgacctg acccatgttt ccttttacat gcccaagacg ctggagacct tggcccaaac
      241 cctggggtta tccccccgct tcacggatgg ggagcggtat ttcgtctggg aaaaagcggg
      301 gacggttggc tgatctcccc aggggcggga tccattccgg cgggggcctg gccgggatcc
      361 gcaggggcgg ttcgtggtgg ccgccaatta ccaaatgtgg taaaattatt accacaaccg
      421 caggggaggc gcgccatggg ttttggcgat catgtcaaag ggcttcgcgt cgggcaaaaa
      481 acgaccttgc ggcggttttg cgtggaccac gggctcgacc ccagcaactg gagcaaggtg
      541 gagcggggcg tccatcctgc cccgaaggat gagcggaccc ttcgggagtg ggccaaatac
      601 ttcggcttga agccccattc cgaggggtgg gaccggttca tggacgaggc ccgtatctcc
      661 cggggcgaga tccccaagga catcctgaag gacgaggaag tcttgtccct gctccccgcc
      721 ttttttcgga ccgtccgggg cgtcgggatc ggcggtccgg aagccgaaag gctgatcgaa
      781 aagatcaggg aagcgaaccg gcccggcaac cccacgcccc ggttgcaaaa gaccgtcaag
      841 aagatccggt tggggaaggc cgcggatcgg gacgacgtgg aatattggct cagccgtccg
      901 gcggccgaac ggatcgaggc ggtggaactc ctgaggaagg agcgttatgg aagctcagcc
      961 cgacttcaag gaacttttcg cgttattgaa ccggcataag gccgaattca tcattgtggg
     1021 cgcctatgcc ctggcctacc acgggatccc ccggaacacc ggggacatcg acgtttatgt
     1081 gcgcccgacc gccttgaacg cgcgccgcgt cctggcggcc ttgggtgatt ttgggttcaa
     1141 ggccctgggg ttgaccgagg ccgatttcca aaggccgggg aacatcatcc agttgggggt
     1201 cccgccggtc cgggtcgatt ttttaacggc cctttccgga gtgacttgga aagaatccga
     1261 ccaagggaaa gccgcgggaa attacggcgg tgtgaaggtc cattacctgg gccgggccca
     1321 attggtggcg aataaacgca agacggcgcg caagaaagac ttggcggact tagaggccct
     1381 gggggaaggc tgaaaacttg gcgcggcggc cgaacccata ggccgaaaaa aacccttcga
     1441 tcccaaccgg tctcggataa accggccgat tgtcgcctaa ttctccgcca caagggggtt
     1501 gtggctcccc catttccggg tacaataagc gcccctgaag gccctaactg ggcactgggc
     1561 aggcttcctt cccgagggta gaatcagggc ctcggagggt tagtcctaat tggtaaggca
     1621 gtggacttga aattctttca gggcttttcc gaaatttccg actaaaaatc tcagtcccca
     1681 gcacataaac aaaagccttt gcctgcaatg gtttcgcggg caaaggcttt ttttatttcg
     1741 acggttggtg atggtcgccg acgagaaaga acccgaaaag atgggttcca atgacacggt
     1801 tatgacacag gcagggaagt ggacttccgc cattgcctga tagggcttta tgggcagtgg
     1861 accccttccc gtgtcattca gcccgggatc cgccttggag aggaacgggt tttgccgaaa
     1921 tccgttaggc cttaccggcc tccggttcca attgaaatga agccggcctg ttcttca
//
LOCUS       DASTJJ010000014         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_369641, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000014 DASTJJ010000000
VERSION     DASTJJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369641"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFR02_00140"
     CDS             <1..269
                     /locus_tag="VFR02_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868895.1"
                     /translation="GLPMGVVKNRFINQLLQLPGEWAALEGRFRSDPPRFFVLRDTDE
                     VPPPPDWLRAALERDYRLEESMAGFELYVRRPGPPAFPVKPGRG"
     gene            356..1669
                     /locus_tag="VFR02_00145"
     CDS             356..1669
                     /locus_tag="VFR02_00145"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9868896.1"
                     /translation="MSQPLTLDLTYIHGVVPGKDLEAARPKAEAALKTLLDHHGRGSD
                     FLGWIGLPFEVEKQLPALLKTAGELAGLDAVICIGIGGSYLGARATIEALGKGKPEIH
                     YLGNHLHATYYADLMDKLEGKQVGLYVISKSGTTTEPAVAFRLLKPWVEKAAGKAGAK
                     RRIVAITDQAKGALKTLADAEGYATFVIPDDVGGRYSVLTPVGLLPIASAGVDIAQLL
                     KGAQAASKDCASGWDTNPALRYAAARQCLYAKGKTIEVLANFRPDFHYISEWWKQLYG
                     ESEGKEGKGAFPASVDLTTDLHSMGQYLQEGRRDLYETILWVAKEERDLAVPSDPDDR
                     DGLNFLAGKPLSWVNEQAMKGTAVAHQDGGLPVIRVTMEKLDAFSLGYLYYFFEMACG
                     VSGTMLGINPFDQPGVEAYKKNMFALLGKKGFEPLLAELRKKGVQ"
BASE COUNT          419 a          732 c          720 g          390 t
ORIGIN      
        1 cgggcctgcc catgggcgtg gtgaagaacc gattcatcaa ccaactgttg cagctgccgg
       61 gcgaatgggc cgcgttggaa ggcaggttcc gttcggaccc gccgcgcttc ttcgtcctgc
      121 gggacacgga cgaggtcccg ccccctcccg attggttgcg ggcggccctg gaacgtgatt
      181 accggttgga ggaatccatg gcgggcttcg agctttatgt ccggcgcccg ggtccccccg
      241 cttttcccgt gaaacccggg cggggttagc ggcttcggga aaaagcggct tgaaatcgac
      301 ccctccgggg gatttaatca aggcctgtgc ccatcccacc cccgaaggag ctttcatgtc
      361 ccaacccctg accctcgacc ttacctatat ccacggcgtc gtgcccggga aggacctgga
      421 agccgcccgg cccaaggcgg aagccgcctt gaagaccctg ctggaccacc atggccgggg
      481 cagcgacttc ctgggctgga tcggcctgcc cttcgaggtg gaaaagcagc tgcccgccct
      541 gctcaagacc gccggggaac tggcggggct tgacgcggtc atctgcatcg gcatcggcgg
      601 gtcctacctg ggcgcccggg ccaccatcga ggccttgggc aagggcaagc ccgagatcca
      661 ctacctgggc aaccacctgc acgccaccta ttacgccgac ctgatggata agctcgaggg
      721 caagcaggtg gggctctacg tcatctccaa gtcgggcacc accaccgagc ccgcggtggc
      781 cttccgcctt ctcaagccct gggtggaaaa ggccgcgggc aaggccgggg ccaagcggcg
      841 catcgtggcc atcacggacc aggccaaggg cgccttgaag acgctggccg acgccgaggg
      901 ctacgccacc ttcgtcatcc ccgacgacgt gggggggcgc tactcggtcc tgacccccgt
      961 ggggcttttg cccatcgcct cggcgggcgt ggacatcgcc caactcctca agggcgccca
     1021 ggccgcctcc aaggactgcg cctcgggctg ggacacgaac ccggcgctgc gctacgcggc
     1081 ggcccggcag tgcctctacg ccaagggcaa gaccatcgag gtgctggcca atttccgccc
     1141 ggacttccac tacatcagcg agtggtggaa gcagctttac ggcgaatccg agggcaagga
     1201 aggcaagggc gcctttccgg cctcggtgga cctcaccacc gacctgcaca gcatgggcca
     1261 gtacctgcag gaaggccgcc gcgacctcta cgagaccatc ctgtgggtgg cgaaggaaga
     1321 acgcgacctg gccgtgccct ccgaccccga cgaccgggac ggcctcaact tcctggcggg
     1381 caagccgctc tcctgggtga acgagcaggc catgaagggc accgcggtgg cgcaccagga
     1441 cgggggattg ccggtgatcc gcgtgacgat ggaaaagctg gacgccttca gcctgggcta
     1501 tctctactat ttcttcgaga tggcctgcgg cgtgtcgggc accatgctgg gcatcaatcc
     1561 cttcgaccaa cccggcgtcg aggcctacaa gaagaacatg ttcgccctgc tcggaaaaaa
     1621 aggcttcgag cccttgctgg cggagttgcg gaagaagggc gtccagtaag cccctggtcc
     1681 gcgtcgggcg gtctccctct ccccttacgg gagagggcgg ggtgaggggt ggtggtcgat
     1741 cccttcggga cctcgaaagg gttgaattgt ttttggccga gtgccaagag gaggacggaa
     1801 agcccagtca agcgcccggc ttgcctgcgg gtcaaaccct gggcggacgg gtttgggcct
     1861 agggtgtttc cccttgttaa cttatttttt acgggcctga gatttaacac aaagccctta
     1921 tggaataagg cttttccccg agcctccggc ggacatttcc tttgacaaca ctaaggctgg
     1981 tggtatataa aacgcagaca gactaaatgt ggggtcaaaa cccccatttt tctggttttt
     2041 ttggcgccat ggggagccgt gccggcgcca aatcgacacc gccaaggggg accagccgta
     2101 ccgttgtagg ggggataccg cgaaagccag ccttaactac tagtcagcca gcccagagcc
     2161 gccgataccc attcgacaac ccattttccg atcaggacga taagggcccc gccgtcttca
     2221 aagcggccgg cgcctttcag gggagacttg ccccccggag c
//
LOCUS       DASTJJ010000015         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_369675, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000015 DASTJJ010000000
VERSION     DASTJJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369675"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFR02_00150"
     CDS             complement(<1..718)
                     /locus_tag="VFR02_00150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9868897.1"
                     /translation="MNVRPQLASIRARYGHPYHQWMTQEFDELGWKLVQKSLLQKRFH
                     DVTLLIEGPDKRFALMSKHSYPPGVFRSPSGGVEPGEDLATGALREAMEETGLPVDLR
                     RFLHHITLDIVFEKDVARWDSYIFYATTKETEIKPRDLKEVREAQWATRSQLELMVHR
                     LRDTGNGGLLYRGNLTEASLWALDNNLRIREARVQDMPSISRTLTANLQDTSAMDRTL
                     WWVAEVTGAWAGTVGIMAESD"
     gene            717..3023
                     /locus_tag="VFR02_00155"
     CDS             717..3023
                     /locus_tag="VFR02_00155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET9868898.1"
                     /translation="MEGSRRVRGWLPFILTARLKGGYEDLKRFLPAFPFLLFLVTTAL
                     SWSSASADKGPADPASWVNPFVGTANGGGTFPGAVAPWGLVSVSPTNDPQAPSGYVSG
                     RPRIFGFGHLHLSGPSEPALGNVVLMPMTGPVKPGAADRASAYGSEQASPGYYGVRLT
                     DWGVTAEMTAALRSGFSRYVFPARAGDADILVDASARLADGRAPKGPWGGSWVRVVSD
                     REVEGASAAGAPGGPVGYFTARFSKACEQSGTWKDGRLSADPQQQGAEVGAFLRFSTA
                     EGEPIDVKVGVSFVDLAGARANLAGELPDWDFEQAVRRARLAWDRQLSRITAEGGEDG
                     ARQVFYTALYHCLLDPPVFSDLDGRYRGFDGSGIQTARGYTRHSFFDLDASRRCLNPL
                     LALAYPGVELDLDKSLVEMGKEGRRLPRAEAAGVETGEGVGAPAAVVLADDWLKGLTG
                     FDVPAAVTEVQKALGPDADGPYVHARDFLRLGYVPRDAADGSPAPWGSVSATLAYAQA
                     YWALGRWAGALGLDGLSKDSLARAGAYAEVFDPKTHFPRPRLREGPFVEPFDPQAGCC
                     DQPWPGSGGPGFYRGTAWQNRFDVPQDLEGLKRLLGGDAGFAAQLEQLLDGQFDPGNP
                     ADFEAPYLFDDLPGREGEAQDWVRRLMARDFGPNADGLPGRDRGGSLSAWYVFSAMGF
                     YPACPATPDYWLGSPLFQKVDVEVDKAFYAGDDLVVKTLDNSPRHPRVQSFAVNGLPQ
                     KALRVTHDQLTRGGVLSLCLGPDPAAGP"
BASE COUNT          412 a         1008 c         1103 g          525 t
ORIGIN      
        1 agtcgctctc cgccatgatg cccaccgtgc cggcccaggc gccggtcacc tcggccaccc
       61 accagagggt gcggtccatg gccgaggtgt cctgcaggtt ggccgtcagg gtgcgggaaa
      121 tggagggcat gtcctggacg cgggcctccc ggatgcgcag gttgttgtcc agggcccaga
      181 gcgaagcctc ggtcaggttg ccccggtaga ggaggccccc gttgcccgtg tcccgcaggc
      241 ggtgcaccat cagctccagc tgggaccggg tggcccattg ggcctcccgc acttccttca
      301 ggtccctcgg cttgatctcg gtctccttgg tggtggcgta gaagatgtag gaatcccagc
      361 gggccacgtc cttctcgaag acgatgtcca gggtgatgtg gtgcaggaaa cggcgcaggt
      421 cgaccggcaa cccggtctcc tccatcgcct cacgcagggc gccggtggcc aggtcctcgc
      481 cgggctccac gccgcccgag ggcgaacgga agacgcccgg gggatagctg tgcttgctca
      541 tgagggcgaa gcgcttgtcc gggccctcga tcaataaagt cacgtcgtgg aagcgttttt
      601 gaagtaaact tttttggacc agcttccagc ccagttcgtc gaactcctgg gtcatccatt
      661 ggtggtaggg atgcccatag cgggcgcgga tggaggccag ttggggtcga acattcatgg
      721 aaggctcccg gcgggttcgt ggttggcttc cttttattct aacagcccgg cttaaaggtg
      781 ggtatgaaga cttgaaacga tttttgccgg cgttcccatt cctcctgttt ctcgtgacaa
      841 cggcattgtc gtggtcttcg gcctccgccg ataaggggcc cgcggacccg gcgtcctggg
      901 tcaacccctt cgtgggcacc gccaacggcg gcggcacctt tccgggcgcg gtggcgcctt
      961 ggggcttggt gtcggtgagc cctaccaacg atccgcaggc cccctcgggt tacgtctcgg
     1021 gccgcccccg cattttcggg ttcggccatc tgcacttgag cggcccctcc gagcccgcgt
     1081 tggggaacgt ggtgctgatg cccatgaccg gcccggtgaa gccgggggcg gcggaccggg
     1141 cctcggccta cggttcggag caagcctccc cgggctatta cggggtgcgc ctgacggact
     1201 ggggcgtgac ggccgaaatg accgccgcct tgcgatcggg gttcagccgt tatgtttttc
     1261 cggcccgcgc gggggacgcc gacatcctgg tggacgcctc ggcgcgcctg gccgacggcc
     1321 gggcgcccaa ggggccctgg ggcgggtcct gggtgcgggt ggtgtcggac cgggaagtgg
     1381 agggcgccag cgccgccggc gcccccgggg ggccggtggg ctatttcacg gcccgcttca
     1441 gcaaggcctg cgagcaaagc ggcacctgga aggacggccg gctctcggcc gatccccaac
     1501 aacaaggcgc ggaggtgggc gccttcctgc gtttttcaac cgccgaaggc gaacccatcg
     1561 acgtgaaggt gggggtctcc ttcgtggacc tggcgggcgc ccgggccaac ctggcgggtg
     1621 aattgcccga ttgggatttc gagcaggccg tccggcgggc ccggctcgcc tgggaccggc
     1681 agttgtcgcg catcacggcc gagggcggcg aggacggcgc ccggcaggtc ttttatacgg
     1741 ccctctacca ttgcctgttg gacccgccgg tcttcagcga cctggacggc cgctaccggg
     1801 gcttcgacgg atcgggcatc cagaccgccc ggggctatac ccgccattcc ttcttcgact
     1861 tggacgcctc ccggcgctgc ctgaacccgc tcttggccct ggcctatccc ggggtggaac
     1921 tggacctgga caagagcctg gtggagatgg gcaaggaagg gcgccggctg ccccgcgccg
     1981 aggcggcggg cgtggagacg ggcgagggcg tgggggcgcc ggcggcggtg gtgctggccg
     2041 acgactggtt gaagggcctg accggattcg acgtcccggc ggcggtcacc gaggtccaaa
     2101 aggccctggg cccggacgcg gacgggccct atgtccacgc gcgggacttc ctccggttgg
     2161 gctatgtgcc ccgggacgcg gccgacggtt ccccggcgcc ctggggttcg gtgtcggcca
     2221 ccctggccta tgcccaggcc tattgggccc tgggccggtg ggccggggcg ctggggctgg
     2281 acggcttgtc caaggattcg ctggcgcgcg ccggggccta cgcggaggtc ttcgacccga
     2341 agacccattt tccccggccc cgcctgaggg aggggccctt cgtggaaccc ttcgaccccc
     2401 aggcgggctg ttgcgaccaa ccctggcccg gcagcggcgg gccggggttt taccggggca
     2461 cggcctggca gaaccgtttc gacgtgcccc aggacctgga aggcctgaag cgcctcctgg
     2521 gcggggacgc gggcttcgcc gcccagctgg aacaattgct ggacgggcag ttcgatccgg
     2581 ggaacccggc ggacttcgag gcgccttatc tcttcgacga cctgccgggc cgggaagggg
     2641 aggcgcagga ctgggtccgc cgattgatgg cgcgcgactt cggccccaac gcggacggcc
     2701 tgccgggccg ggaccggggg ggcagcctct cggcctggta cgtgttctcg gccatgggct
     2761 tttatcccgc ctgcccggcc acgccggact attggttggg cagcccgctt ttccagaagg
     2821 tcgacgtgga ggtcgacaag gccttctacg ccggggacga cctggtggtg aagaccctgg
     2881 acaattcccc ccggcatccc cgggtgcagt ccttcgcggt caacggcctg ccccaaaagg
     2941 ccttgcgggt gacccacgac caattgaccc ggggcggggt cctgtccctg tgcctggggc
     3001 cggaccccgc cgccggcccc taaaaacggg cttttccgcg cccctcgg
//
LOCUS       DASTJJ010000016         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1470887, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000016 DASTJJ010000000
VERSION     DASTJJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470887"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /gene="ffh"
                     /locus_tag="VFR02_00160"
     CDS             <1..1253
                     /gene="ffh"
                     /locus_tag="VFR02_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9868899.1"
                     /translation="DTVKLFLDRVKAKALGQDVALSLTPGQVMIKIVQEELTSLLTPK
                     DRNAVKLRFAPAPPTLILLAGLQGTGKTTTAAKLALYLKKDGRKPGLVAADLVRPAAI
                     DQLEVLAKEVGVPFFRPEGGEDALALCQRAVKGSLAAGCNLLILDTAGRLQVDEAMMA
                     EITAIQQKLKPSNVLLVVDAMTGQNALQVAAAFHARVPLTGLVLTKADGDSRGGAVLS
                     ITSFTGVPIQFTGVSEKMEGLDPFYPDRMASRILGMGDVLGLIEKAEAAARDAAFQKQ
                     KLEKASDFNLDHLLDQLRQIQKMGSMQDLISMMPGGAQAKQQMAAGAPDEKRLKRMEA
                     ILLSMTPKERKFPQILDGSRRRRIAQGSGTRADEVNSLLKQYDMMKKVMKKPGLLQKM
                     QQMGGGTGMPPGGFPGMPPGFPRI"
     gene            1429..>1729
                     /locus_tag="VFR02_00165"
     CDS             1429..>1729
                     /locus_tag="VFR02_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9868900.1"
                     /translation="MALFGSKDDKKKELIESAQKLAEQGDSSKALKEIQKALDIDPNY
                     REAYTAMGFIYSDLGQAEDAITAFKKATVIDHSSTEAWNNLGLAYARAERYNDAIK"
BASE COUNT          357 a          601 c          509 g          262 t
ORIGIN      
        1 tggacaccgt caagctgttc ctggaccggg tcaaggccaa agccctgggc caggacgtcg
       61 ccttgagcct cacccccggc caggtcatga tcaagatcgt ccaggaagag ctgacctccc
      121 tgctgacgcc caaggaccgc aacgcggtca agctgcgctt cgcccccgcg ccgcccacgc
      181 tgatcctgtt ggccggcctg cagggcaccg gcaagaccac caccgccgcc aagctggcgc
      241 tctacctcaa gaaggacggc cgcaagcccg gcctggtggc cgccgacctg gttcgccccg
      301 ccgccatcga ccaactggag gtgctggcca aggaagtggg cgtccccttc ttccggcccg
      361 aagggggcga ggacgcgctc gccctctgcc aaagggccgt caagggctcc ctggccgccg
      421 gctgcaacct gctcatcctg gacacggcgg gccgcctgca ggtggacgag gccatgatgg
      481 ccgagatcac ggcgatccag caaaagctca agccctccaa cgtgctcctg gtggtggacg
      541 ccatgacggg ccagaacgcc ctgcaggtgg ccgccgcctt ccacgcccgc gtgcccctga
      601 ccggcctggt cctgaccaag gccgacggcg actcgcgggg cggcgccgtg ctttccatca
      661 cttccttcac gggcgtgccc atccagttca cgggcgtcag cgagaagatg gagggcctgg
      721 acccttttta tcccgaccgc atggcctccc gcatcctggg catgggcgac gtgctggggt
      781 tgatcgagaa ggccgaggcc gccgcccggg acgccgcctt ccagaaacag aagctcgaga
      841 aggcctccga cttcaacctg gaccacctgc tggaccaatt gcgccagatc cagaagatgg
      901 ggtccatgca ggacctcatc tccatgatgc cgggcggcgc gcaggccaag caacagatgg
      961 ccgccggggc gcccgacgaa aaacgcctca agcgcatgga ggccatcctg ctgtccatga
     1021 cgcccaagga acgcaagttc ccgcagatcc tggacggttc ccgccgtcgc cgcatcgccc
     1081 aaggcagcgg cacccgggcc gatgaggtga acagcctgct caagcaatac gacatgatga
     1141 aaaaggtcat gaaaaaaccc ggcctgctcc aaaaaatgca gcaaatgggg ggcggaacgg
     1201 gcatgccgcc cggcggcttc cccggcatgc cccccggatt cccaaggatt tgaaccccat
     1261 tcccccacca aaaatgcttt tggccttcct tcgtggactt cgtggccccg tcaggggctc
     1321 gtggtgaacg atgggttttt ccttgttttt cccgcattga cctttaaaaa gcgcaggggc
     1381 tagaatgtgc gctccaccct gttttgaacc aaggtaaacg gtacctccat ggcccttttc
     1441 ggaagcaagg acgataaaaa gaaagaactc atcgagtccg cccagaagct ggcggaacaa
     1501 ggcgacagct ccaaggccct caaggagatc caaaaagccc tcgacatcga ccccaattac
     1561 cgggaagcct acacggccat ggggttcatc tactcggacc tgggccaggc cgaggatgcc
     1621 atcaccgcct tcaagaaggc caccgtcatc gaccacagct ccaccgaggc ctggaacaac
     1681 ctgggcctgg cctacgcccg ggccgaacgc tacaacgacg ccatcaagg
//
LOCUS       DASTJJ010000017         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_2810, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000017 DASTJJ010000000
VERSION     DASTJJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFR02_00170"
     CDS             <1..357
                     /locus_tag="VFR02_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9868901.1"
                     /translation="SEVVLWGTGAPRREFLYVDDLAEACVFLLGLADPPDWVNVGVGE
                     DATIRDLAGLVAKTVGYGGSVRWDPSKPDGTPRKLMDVSRLAAAGWKARVGLEEGIGL
                     AYADYLAAKDSGRLRQ"
     gene            430..1173
                     /locus_tag="VFR02_00175"
     CDS             430..1173
                     /locus_tag="VFR02_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET9868902.1"
                     /translation="MLTLIADVDDTVCPSTRPISPALAREIDRLVSGGCAFAFISGST
                     IPQISAQITPFLTVPHHLLGASGTHYVRVDRAEGKASPVEVYRLEFSPEERAEILRAF
                     EALIEKYAIRSLTDKADQLQDRSSQITLSAVGRHAPEAAKRALDPDGVKRREWVGFLR
                     GLLGDKYSLRVGGTTSIDVTPAGVDKAWGIRRFLKENGLRASECLFFGDKLGPGGNDA
                     PALEVVDCLEVRGPDHTLEILSRFKTGAR"
BASE COUNT          185 a          439 c          445 g          217 t
ORIGIN      
        1 tccgaggtgg tgctttgggg cacgggtgcc ccgcgccggg aattcctgta cgtggacgac
       61 ctggccgagg cctgcgtttt cctcctgggc ctggccgatc cgcccgactg ggtgaacgtg
      121 ggggtggggg aggacgcgac catccgggac ctggccggcc tggtggccaa gacggtgggg
      181 tacggcggct cggtccgctg ggacccttcc aaacccgacg gcacgccgcg gaagctcatg
      241 gacgtctccc ggctcgccgc cgccggctgg aaggcccgcg tgggcctgga ggaggggatc
      301 ggcctggcct atgcggacta cctggccgcg aaggactcgg gccgcctgcg ccagtaggcc
      361 ccggatgttt aagctgaaag gccgggccgg tttttgttaa agtggcccat cccgcccgga
      421 aaggatccca tgctgaccct gatcgccgac gtcgatgaca ccgtctgccc cagcacccga
      481 ccgatctccc ccgccctggc ccgggagatc gaccggttgg tctcgggcgg ctgcgctttc
      541 gccttcatca gcggcagcac catcccccag atcagcgccc agatcacgcc cttcctcacc
      601 gttccgcacc acctgttggg ggcctcgggc acccattacg tgcgcgtgga ccgcgcggaa
      661 gggaaggctt cccccgtgga ggtctaccgg ttggaattca gccccgagga acgcgccgag
      721 atcctgcggg ccttcgaggc cctgatcgaa aagtacgcca tccgctcctt gaccgacaag
      781 gccgaccagt tgcaggaccg cagttcccag atcacccttt cggccgtcgg acgccacgcg
      841 cccgaggcgg ccaagcgggc cctggacccg gacggcgtca aacgccggga atgggtggga
      901 ttcctgcggg gcctgttggg cgacaaatat tccctgcggg tgggcggcac cacctccatc
      961 gatgtcaccc cggcgggcgt ggacaaggct tggggcatcc gccgtttcct gaaggagaac
     1021 ggcttgcggg cctccgagtg cctctttttc ggcgacaagc tgggcccggg cggcaatgac
     1081 gcccccgccc tggaggtggt ggattgcctg gaggtgcgcg gcccggacca taccttggag
     1141 atcctctccc ggttcaagac cggggcccgg tagcgccttg ggggggaccg gtttctggga
     1201 acggttgcgt gccggatcgc ggcggctgag gaccccggcc ctggcgttcc tggccttgtt
     1261 gaccctccag gccttctatt acgccg
//
LOCUS       DASTJJ010000018         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_125585, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000018 DASTJJ010000000
VERSION     DASTJJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125585"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="VFR02_00180"
     CDS             complement(<1..>1068)
                     /locus_tag="VFR02_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868903.1"
                     /translation="FNSISVSNPTPLAGSVIGVTVVYCIGTFDDPCFRVGLNPNFTTL
                     QPCPAANQVFVVDTNTTPTQTSPVSSSVADTSDGGKGWEGVPTSGTALCPTTQVFNVT
                     IPASLSGGTYNLIVEEQGYYAACASGSNASTYTTITIPLPPAAVTVTKSAGTTVASPA
                     SLILFNLNYSYVNTTNVVLTDQVPANTTLVQVGPGGSSGGSSPGSTITWNLGPAVAGA
                     AVTGTAWFLGRVDAAAADGTVITNTASSSSDQVASAPSNSVAVTVKSPTMVLTKSESA
                     VSAAAGTPVTYALNWSALGSSLQIYDSYNNDTVGTSNGSIAGYDNTVYTRYAASTGDL
                     GTWQVGTDSSGNHFVEGKVST"
BASE COUNT          190 a          311 c          371 g          196 t
ORIGIN      
        1 aagtcgaaac ctttccttcg acgaagtggt tccccgatga atccgtgccc acctgccagg
       61 tgcccaagtc gccggtggaa gcggcgtagc gggtataaac ggtgttgtcg tatccggcga
      121 tggaaccgtt gctggtcccc accgtatcgt tgttgtagga atcgtaaatc tgcaggctgc
      181 ttcccaaagc gctccagttc aacgcgtagg tcaccggagt gcccgccgcc gccgagaccg
      241 cggattcgct tttggtcaat accatggtgg gcgacttgac cgtgaccgcc accgagttgc
      301 tgggcgcgga ggccacttgg tccgaagagg aggaagccgt gttggtgatg acggtcccat
      361 cggcggcggc ggcatccacc ctgcccaaga accaggccgt ccccgtgacg gcggcgcccg
      421 ccaccgccgg ccccaggttc caggtgatgg tactgcccgg cgaactgccg cctgacgagc
      481 cacctggccc cacctgcacc agggtggtgt tggccggcac ctggtcggtc aggaccacgt
      541 tggtggtgtt gacatagctg taattgaggt tgaagaggat caggctggcc ggggaggcca
      601 ccgtggtccc ggcgcttttg gtgaccgtga cggccgccgg cggcaggggg atcgtgatgg
      661 tggtataggt gctggcgttg gatcccgagg cgcaggcggc gtaatatccc tgttcctcga
      721 cgatcaggtt gtaggtcccg cccgacaaag aggcggggat ggtgacgttg aagacctgtg
      781 tggtcggaca aagcgccgtc ccgctggtgg ggacgccctc ccaacccttg ccgccgtccg
      841 acgtgtccgc cacggaagaa ctgacgggac tggtttgggt gggggtcgtg ttggtgtcca
      901 ccacgaagac ctggttggcc gcgggacagg gctgcaaggt ggtgaaattc gggttcaacc
      961 ccacgcggaa gcaggggtca tcgaaagtcc cgatgcaata aaccaccgtg accccgatca
     1021 ccgacccggc caagggggtc gggttggaaa cggaaatgct gttgaagg
//
LOCUS       DASTJJ010000019         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1348812, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000019 DASTJJ010000000
VERSION     DASTJJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348812"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFR02_00185"
     CDS             complement(<1..447)
                     /locus_tag="VFR02_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9868904.1"
                     /translation="MILKIIDWCARNKFLVLLTTAFLTGWGIYSLKTIPLDAIPDISD
                     TQVVVFAQWDRSPDILEDQVATPIVRALLGAPRVKAIRAASDFGYTYVYVIFQDGTDI
                     YWARSRVLEYLSKVQSDLPQGVKVQLGPDASGVGWVYEYALVDKTGQ"
     gene            complement(450..>1125)
                     /locus_tag="VFR02_00190"
     CDS             complement(450..>1125)
                     /locus_tag="VFR02_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868905.1"
                     /translation="PSEASSGLPDALKGLAPIDISPFQAKMIDVKLAQVRMAPVIRVI
                     RTVGRFAGGANDFSSLALDFAAEGKVGASQARYVVADVYALDLPFVKVGQKALVSSLN
                     GGGAQEGVVSKIYPYDGTQSRVTRVKIRLAKPVPDEIYANVEIEASTAPKLSVPREAV
                     LSTGTQSYVFVGDGEGHFVPRAVQVGFKGDDLFQITSGLKEGDPVAWGGTFLLDADAH
                     LQAGEE"
BASE COUNT          239 a          363 c          325 g          198 t
ORIGIN      
        1 ctgtcccgtt ttatccacca gcgcgtactc gtagacccag cccacgccgc tggcgtccgg
       61 cccgagctgg accttcacgc cctgcggcag gtcgctttgc accttcgaaa gatattccag
      121 cacccggctc ctggcccagt aaatatcggt gccgtcctgg aatatgacgt agacataggt
      181 gtaaccaaaa tcggaagccg cccggatggc cttcacccgg ggcgcgccca gcagggcccg
      241 cacgatgggg gtggccacct ggtcctccaa aatatcgggg cttcggtccc attgggcgaa
      301 gaccaccacc tgggtatccg agatatccgg gatggcgtcc aaggggatgg tcttgagcga
      361 atagatgccc caccccgtta aaaaagccgt cgtgaggagg accaagaatt tgttcctggc
      421 gcaccagtcg atgatcttaa ggatcatggc tattcctccc ccgcttggag gtgggcatcc
      481 gcgtccaaca ggaacgtgcc cccccaggcc accggatccc cttctttcaa tcccgaggtg
      541 atctggaaaa gatcatcccc cttaaaaccc acctggaccg cccgggggac aaaatgacct
      601 tccccatccc caacaaagac ataggactgg gtaccggtcg aaaggaccgc ctcccgggga
      661 acggaaagtt tcggcgccgt ggacgcctcg atctccacat tggcgtaaat ttcgtccggt
      721 acgggcttgg caagccgtat cttcacgcgg gtcacccggg attgggtgcc gtcgtaaggg
      781 tagatcttgg aaacgacccc ctcctgggca ccgccgccgt tcagtgagga gaccaacgcc
      841 ttctgcccaa ccttcacgaa gggcaggtcc agcgcataaa cgtcggccac gacatagcgg
      901 gcctgggaag cccccacttt cccttcagcg gcgaaatcca aggcgaggga cgaaaaatcg
      961 ttggcgccgc ccgcgaaacg ccccaccgtg cggatgaccc ggatcaccgg ggccatccgc
     1021 acttgggcca atttcacgtc gatcatcttg gcttggaagg gggaaatatc gatgggcgcc
     1081 agacccttga gggcgtcggg aagcccggaa gaagcttcgg acggc
//
LOCUS       DASTJJ010000020         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1471255, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000020 DASTJJ010000000
VERSION     DASTJJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471255"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFR02_00195"
     CDS             complement(<1..406)
                     /locus_tag="VFR02_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9868906.1"
                     /translation="MSEPRLILFDIDGTILSTDRKAWEDPFRDAMEAALHEIGEPQKI
                     DTSRYKSGGKTDTQIIYEILDANGFPEETIARVFPAVQKKYLQLLREVVEANPQYVIL
                     KPGVVELLEELKRMPHLVLALLTGNFEEGARIK"
     gene            552..>1423
                     /locus_tag="VFR02_00200"
     CDS             552..>1423
                     /locus_tag="VFR02_00200"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HET9868907.1"
                     /translation="METPPSAARPFPWALGLAGLSAIWVGVGFGRFSFTQFIPALVVE
                     GWFPVAQADYLGAANLVGYLLGSLLAGLLARHYSVPWLLRGAMLACAAGFLLCAHPGP
                     FVWFAFWRFIVGVAGAILMILAPPTLLAEAAEPHRHPLRGLIFSGLGLGILFSAAALP
                     LLLRGGLAWAWAGLGGLSLFLALAFWRTWPPPLSLVDPEGAGFRMTKPLWVLLVLYCC
                     SATGLVPCTVFWVDYMARGLHLGMAVASFNWLLFGLGSLAGPFVGSWLGARLGFHRGI
                     PVSLLGMAAANLLA"
BASE COUNT          156 a          482 c          468 g          317 t
ORIGIN      
        1 gcttgatgcg ggccccttcc tcgaaattgc cggtcaagag cgccagcacc aggtggggca
       61 tgcgcttgag ttcctccaac agctccacca cgcccggctt caggatgacg tactgggggt
      121 tggcctccac gacctcgcgc aagagctgca ggtacttttt ttgcaccgcc gggaagaccc
      181 gggcgatggt ctcctcgggg aatccgttgg cgtccaggat ctcgtagatg atctgggtgt
      241 cggtcttacc gcccgacttg tagcgggacg tgtcgatctt ttgcggctcg ccgatctcgt
      301 gaagggccgc ctccatggcg tcgcggaagg ggtcctccca ggccttgcgg tcggtggaca
      361 ggatggtgcc gtcgatgtcg aacaggatca ggcgtggctc gctcataggc cgcctagtgt
      421 agcaggatgg tgaaaaagtc caagaggcgg attttcctgt ccggcccgcg ccggggatct
      481 tcgggggggc tttctttccg tccgggcgct ccctagagtg tggacacttc caagccgccc
      541 aaaggaacgc catggaaacc cccccgtccg ccgcccgacc ctttccctgg gccctgggac
      601 tcgccggcct ttcggccatc tgggtgggcg tgggcttcgg ccgtttttcc ttcacccagt
      661 tcatccccgc cctggtggtg gaaggttggt tccccgtggc ccaggccgac tacctgggcg
      721 ccgccaatct ggtgggttat ctgctgggat ccttgctggc gggcctcctg gcccggcatt
      781 attcggtgcc ctggctgctg cgcggcgcca tgctggcctg cgcggccggt ttcctgctct
      841 gcgcgcatcc cggacccttc gtctggttcg ccttctggcg tttcatcgtg ggcgtggcgg
      901 gcgccatcct gatgatcctg gccccaccca ccctcttggc cgaagccgcg gagccccacc
      961 gccacccgct ccggggcctc atcttctcgg ggttgggatt gggcatcctt ttctccgccg
     1021 ccgccttgcc actgctcctg cgcggggggc tcgcctgggc ctgggcgggc ctgggcggcc
     1081 tgtcgctctt cctggccctg gctttctggc gcacctggcc gcccccgctg tcgctggtgg
     1141 accccgaagg cgcgggcttc cgcatgacga aacccctctg ggtgttgctg gttctctatt
     1201 gctgcagcgc gaccggcctc gtcccctgca cggtcttttg ggtcgactac atggcccggg
     1261 gcctgcatct ggggatggcc gtggcttcat tcaattggct tcttttcggc ctggggtcgc
     1321 tggccgggcc cttcgtgggt tcctggctgg gcgcccggtt gggattccac cggggcattc
     1381 cggtcagcct tttgggcatg gcggccgcca acctcctggc cat
//
LOCUS       DASTJJ010000021         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1104407, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000021 DASTJJ010000000
VERSION     DASTJJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104407"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..>2097
                     /gene="gyrA"
                     /locus_tag="VFR02_00205"
     CDS             143..>2097
                     /gene="gyrA"
                     /locus_tag="VFR02_00205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9868908.1"
                     /translation="MKDSYLDYAMSVIVGRALPDVRDGLKPVHRRIFMGLHDLGLTSR
                     KPYRKSAKVTGDVTGNYHPHGTASVYDSIVRLAQDFSMRYPLVDGQGNFGSIDGDPPA
                     AERYTEVRMSPLAEELLRDIDKNTVDFVPNYDNTREEPLVLPSAFPNFLVNGAQGIAV
                     GMATNVPPHNLTEVIDGLVHLIENPQATVKELIGFIKGPDFPTGGYILGREGIVEAYT
                     TGRGSVRMQAKAAIEVDKKDRERIIVSQLPFQVNKAQLIEKIAELVRDKKLEGITDLR
                     DESDREGMRVVIEVSKNMNAQVVLNNLLTHTSLRTSFGIIMLALVNGQPRVLNLKGLL
                     FHYLEHRKEVVIRRTKFDLDKAEKRAHILEGLRIAVDNLDKVIKTIRASKNTEEAKNA
                     LMANFKLSALQAQAILEMQLRQLTNLEIKKIEEEYLEIIKTIERLKGILASEKKLWQV
                     IKEELLEIKTRFGDARRTEILGKAEDIKVEDLIEDEQVVITLSHAGYIKRMPADTYQK
                     QRRGGKGVAGATMREEDFIERMYTASTHDYLLNFTNFGKVHWVKVYEIPESSRYAKGT
                     ALANLLKLDENEKISAVLPVKEFTEKSNVILGTANGLVKKTVLTAFDKPRSGGIIAIT
                     LDKGDKLVSAFITDGKRELFLATRQGK"
BASE COUNT          458 a          728 c          621 g          290 t
ORIGIN      
        1 catggccaag gccaaaaaga aaaaagacga ctcccagacc cccgacgagg cccccaagcc
       61 gggcgcgccc gcgcccgccg cgggcggcgg cggcgacgac ctgaaccggg gccgcatcgt
      121 caccaccgac atcgaggagg agatgaagga ttcctacctc gattacgcca tgagcgtgat
      181 cgtgggccgg gccctgcccg acgtgcgcga cgggctcaag cccgtgcacc ggcgcatctt
      241 catgggcctg cacgacctgg ggctgacctc ccgcaagccc taccgcaagt ccgccaaggt
      301 caccggcgac gtgacgggga actaccaccc ccacgggacc gcctcggtct acgattccat
      361 cgtgcgcctg gcccaggact tctccatgcg ctaccccctg gtcgacggcc agggcaactt
      421 cggctccatc gacggcgacc ccccggcggc cgaacgttac accgaggtgc gcatgtcccc
      481 gctggccgag gaactgctgc gggacatcga caagaacacg gtggacttcg tccccaacta
      541 cgacaacacc cgggaagaac ccctggtgtt gcccagcgcc ttccccaact tcctggtcaa
      601 cggcgcccag ggcatcgccg tgggcatggc caccaacgtg ccgccccaca acttgaccga
      661 ggtcatcgac ggactggtcc acctcatcga gaacccgcag gccaccgtga aggagctgat
      721 cggtttcatc aaggggccgg atttccccac gggcggctac atcctgggcc gggaaggcat
      781 cgtggaggcc tataccacgg gccgcggcag cgtgcgcatg caggccaagg ccgccatcga
      841 ggtcgacaag aaggaccggg agcgcatcat cgtctcccag ctgcccttcc aggtgaacaa
      901 ggcccagctc atcgagaaga tcgccgaact ggtgcgcgac aagaaattgg aaggcatcac
      961 ggacctgagg gacgagtccg accgcgaggg catgcgggtg gtcatcgaag tgtccaagaa
     1021 catgaacgcc caggtggtgc tcaacaacct cctgacccac acatcgctgc gcacctcctt
     1081 cggcatcatc atgctggcgc tggtcaacgg ccagccccgg gtgctcaacc tcaagggcct
     1141 gcttttccat tacctggaac accgcaagga agtggtgatc cgtcgcacca agttcgacct
     1201 ggacaaggcc gagaagcgcg cccatatcct ggagggcctg cgcatcgcgg tggacaacct
     1261 ggacaaggtc atcaagacca tccgcgcctc caagaacacc gaggaggcca agaacgccct
     1321 catggccaac ttcaagctct ccgccctgca ggcccaggcg atcctcgaga tgcagctgcg
     1381 ccagctcacc aacctggaga tcaagaagat cgaggaggaa tacctcgaga tcatcaagac
     1441 catcgagcgc ctgaagggca tcctggcctc cgagaagaag ctctggcagg tcatcaagga
     1501 ggaactcctc gagatcaaga cccgcttcgg cgacgcgcgc cgcaccgaga tcctgggcaa
     1561 ggccgaggac atcaaggtgg aggacctcat cgaggacgag caggtggtca tcaccctgtc
     1621 ccacgcgggc tacatcaagc gcatgcccgc cgacacctac cagaagcagc gccgcggcgg
     1681 caagggcgtg gccggcgcca ccatgcgcga ggaggacttc atcgagcgca tgtacaccgc
     1741 ctccacccac gactacctgc tcaacttcac caacttcggc aaggtgcatt gggtgaaggt
     1801 ctacgagatc cccgagtcgt cccgctacgc caagggcacg gccctggcca acctgctcaa
     1861 gctggacgag aacgagaaga tctcggccgt gctgccggtc aaggaattca ccgagaagtc
     1921 caacgtgatc ctgggcacgg ccaacggcct ggtcaagaag acggtcctca ccgccttcga
     1981 caagccccgc tcgggcggca tcatcgccat caccctggac aagggcgaca agctggtgtc
     2041 ggccttcatc accgacggca agcgcgagct tttcctggcc acccgccagg gcaagag
//
LOCUS       DASTJJ010000022         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_126099, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000022 DASTJJ010000000
VERSION     DASTJJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126099"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="rodA"
                     /locus_tag="VFR02_00210"
     CDS             <1..591
                     /gene="rodA"
                     /locus_tag="VFR02_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET9868909.1"
                     /translation="GVRLRWLLWMFILGVSSLPIIWHFLKDYQRERLNVFLSPQADPL
                     GAGYNLIQSIIAIGSGGVSGKGYLQGTQTQLSFIPEHHTDFIFAVVGEEWGFVGCLVV
                     LGLYYFMIQKSFEIARKARDREGAMLALGLSSIFAVQIVVNVGMTIGILPVTGLTLPF
                     ISYGGTSLVFSYAAIGMLLNVSYANRRPVLVGTGRG"
     gene            760..1239
                     /locus_tag="VFR02_00215"
     CDS             760..1239
                     /locus_tag="VFR02_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868910.1"
                     /translation="MAKRTTKAKAAPKLEVLTWKKGWTLFAACWLATFLAVWGLRQVF
                     IPKEELDCYARVQVLQKAVDQWNAAHPDQAMTDSLDPGQGLDEAKLTAAGLMPAQTYD
                     HNRHYYFIDKTPHGLKVKCNKDEDNPLILRLVGDTLLAALVFVFYCSGRGLVLFKPV"
     gene            1278..1589
                     /locus_tag="VFR02_00220"
     CDS             1278..1589
                     /locus_tag="VFR02_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868911.1"
                     /translation="MTVFNFAGKGPVVLSDPMVLLRLLGAACLLVGTVFLWKPDILKN
                     WWVDKQSLMRSIASPAVNRFLYRLSGSILVVMGLFCLLADQDTVANVVGNIARFLFGD
                     R"
     gene            1621..>1946
                     /locus_tag="VFR02_00225"
     CDS             1621..>1946
                     /locus_tag="VFR02_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868912.1"
                     /translation="MVRWIQSRFEVFQLVVLGTRSFRLLFGLQVLVVALHWLPPAAGT
                     AFLAAVPWWGWVLGWLAYLWLTTLGYAAERKGRFDFTSMNFFKSYLDFIINEGGRLLV
                     DSESKD"
BASE COUNT          302 a          581 c          642 g          421 t
ORIGIN      
        1 ggggtgcggt tgcgctggct tttgtggatg ttcatcctgg gggtgtcgtc gctgcccatc
       61 atctggcact tcctcaagga ctaccaacgg gagcgcctga acgttttctt gagcccccag
      121 gcggacccgc tgggcgcggg ctacaacctg atccagtcca tcatcgccat cgggtcgggc
      181 ggagtgtcgg gcaagggcta cctgcagggc acccagaccc agctttcctt catccccgag
      241 caccatacgg acttcatctt cgccgtggtg ggcgaggaat gggggttcgt gggttgcctg
      301 gtggtgctgg ggctctacta tttcatgatc cagaagtcct tcgagatcgc ccgcaaggcc
      361 cgggaccggg agggcgccat gctggccctg ggcctttcca gcatcttcgc cgtgcagatc
      421 gtggtgaacg tgggcatgac cattggcatc ctgccggtga ccggcctgac cctgcccttc
      481 atcagctacg gcggcacctc gctggtcttc agctacgccg ccatcggcat gctcctgaac
      541 gtttcctacg ccaaccggcg ccccgtgctg gtaggcacgg gacggggtta agtccgtgct
      601 ggtaggcacg gggcggggtt gaagccttgg tcggctccct cgccccttgc gggagagggc
      661 cggggtgagg ggtggcttgg cctttagctg tttttgaagg gcgctgtgta tcgaggcagt
      721 gtggttttgt tatactcggc ccatccataa gggggaagca tggcgaagag aacgaccaaa
      781 gcgaaggccg cgcccaaact cgaggtgctg acctggaaaa agggctggac cctcttcgcg
      841 gcctgctggc tggccacctt cctggcggtc tggggcctgc gccaggtctt catcccgaag
      901 gaagagctgg actgctacgc ccgcgtgcag gtcctgcaga aggccgtgga ccaatggaac
      961 gccgcccacc ccgaccaggc catgacggac tcgttggacc ccggccaggg cctggatgag
     1021 gccaagctga cggccgccgg gctcatgccc gcccaaacct acgaccacaa ccgccactac
     1081 tacttcatcg acaagacgcc ccacggcctc aaggtcaagt gcaacaagga tgaggacaat
     1141 ccgctgatcc tgcgcctggt gggggatacc ctgctggcgg cgctggtctt cgtgttctat
     1201 tgttcgggcc gtggcttggt gctcttcaag ccggtttgac gcaggacgga cgcggggtcg
     1261 gcaaaaggcg tctggttgtg acggtattca atttcgcggg gaaaggtcct gtcgtgttga
     1321 gcgatcccat ggtcttgctg cgcctgctgg gggcggcctg tttgctggtc gggaccgttt
     1381 ttctttggaa gccggatatc ctcaagaact ggtgggtgga caagcagtcc ctgatgcggt
     1441 ccatcgcgag cccggcggtc aaccggttcc tctatcggct ttccggttcc atcttggtcg
     1501 tgatgggcct tttttgcctc ttggccgatc aggacacggt cgccaacgtc gtcgggaaca
     1561 tcgcccggtt cctcttcggc gacagataaa gcccgcccat gggcggatga aggtcgttcc
     1621 ttggtgagat ggatccagtc ccggttcgag gtcttccaac tggtggtctt ggggacccgg
     1681 tcctttcggc tcctgttcgg cttgcaggtc ctggtggtgg ccctccattg gctgccgccg
     1741 gccgcgggta cggccttcct ggctgcggtc ccctggtggg gttgggtcct gggctggctg
     1801 gcctacctgt ggctcacgac cctggggtac gcggcggaac gcaagggacg tttcgacttc
     1861 acctccatga acttcttcaa gtcgtacctg gacttcatca tcaacgaggg tggaaggctg
     1921 ctggtcgatt cggaaagcaa ggactt
//
LOCUS       DASTJJ010000023         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_3462, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000023 DASTJJ010000000
VERSION     DASTJJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..>1292)
                     /locus_tag="VFR02_00230"
     CDS             complement(137..>1292)
                     /locus_tag="VFR02_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868913.1"
                     /translation="HWVVRSAYWIPLACFFAARGAMEGARRLIDDKKDRSGVWALLVG
                     ASLAAALALGVPAGLPSFWAGGLLLLAAGWRGIRPGLRGGLLLAALGCSLLPAVQSFY
                     FTLPQSFYQAPPLRAAAVPGTRIFHAPDLLDATKTVSGSTAQQACGKPKELLMPNWPL
                     LEGLEEAGVNNTLCLREFLRWYYAPQEAPAGSRKKFLDYLNAGLVLGWTAPPGAPRPI
                     AGFPVPVWIDPTPLPKWFCASRARLEGGWPLDFETMAKPGYDFARDCFVADPERVGSY
                     APRPVEMDRPSPNGVELEVGGKGRALLVSSELAYPGWKARVAGEDRGLEVVNHSFRGL
                     ALGSGERRVALRYDPGSVRLGLFGLLLALGLWIFTAWGLLAPGKAAHGRA"
BASE COUNT          255 a          473 c          408 g          156 t
ORIGIN      
        1 atcctccggc gagagggcct tcaccgaggc ctgggtgccc acgggcatgt agcagggggt
       61 ctggaccgtc ccgtggggca gatggagcaa ccccacccgg ccccgggtcc cggggtcggt
      121 cgcgaggacg tcgaacttat gcgcggccat gtgccgcctt tccgggggcg agcaggcccc
      181 acgccgtgaa gatccaaagc cccaaagcca acaacaggcc gaacaggccc aagcggaccg
      241 aaccggggtc ataccgcagg gccacccggc gttccccgga ccccagcgcc agcccccgga
      301 aactatggtt caccacctcc aaaccccgat cctcccccgc cacccgggcc ttccaacccg
      361 gataggccaa ctcactggaa accaacaggg cacggccctt ccccccgacc tccagttcca
      421 ccccgttggg ggaaggccgg tccatttcca ccggccgggg cgcgtaggat cccacccttt
      481 ccggatcggc cacaaaacag tcccgggcaa aatcatagcc gggcttggcc atggtttcaa
      541 agtccaaagg ccatccccct tccagccggg cccgcgacgc gcagaaccat ttggggagcg
      601 gggtcggatc gatccagacc ggcaccggga accccgcgat cggccggggc gcgccgggag
      661 gcgccgtcca accgagcacc aagcccgcgt tcaaataatc caagaatttt ttccgggatc
      721 ccgccggcgc ctcttggggg gcgtagtacc acctcaagaa ctcccgcaga cacagggtgt
      781 tgttgactcc cgcctcctcc aacccctcca gcaggggcca gttgggcatg aggagctcct
      841 tgggcttgcc gcaggcctgc tgggccgtgg agcccgaaac ggtcttggtg gcgtccaaca
      901 ggtccggggc gtggaagatc ctggtgcccg gcacggccgc cgcccgaagg ggtggggctt
      961 ggtaaaagga ctggggcagg gtgaaataaa agctttggac cgccggcagg agggagcaac
     1021 ccaaggccgc caggagcagg ccgccccgca ggccgggcct gatgccgcgc caacccgccg
     1081 ccaggagcaa caggccgccc gcccaaaagg aaggaagccc ggccggcacg cccaaggcca
     1141 aggccgcggc caaactggcc cccaccagga gcgcccaaac cccggaccgg tccttcttat
     1201 catcgatcag gcgccgggcg ccttccatgg cgccccgggc cgcgaaaaaa caggccaagg
     1261 ggatccaata ggccgaccgg accacccaat gg
//
LOCUS       DASTJJ010000024         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1471855, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000024 DASTJJ010000000
VERSION     DASTJJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471855"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="rfbC"
                     /locus_tag="VFR02_00235"
     CDS             <1..330
                     /gene="rfbC"
                     /locus_tag="VFR02_00235"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET9868914.1"
                     /translation="GEIYDVALDGRPDSPTCGRWAAFQLKDSGFQLLYIPPGFFHGFC
                     VLSGPADVAYKCTDYYDRTDEVGVLWNDPALGIAWPLADPVLSEKDRGLPPFARVLPT
                     MAPGPGK"
     gene            393..1067
                     /locus_tag="VFR02_00240"
     CDS             393..1067
                     /locus_tag="VFR02_00240"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HET9868915.1"
                     /translation="MADHSFDKYARLLFKHHPWHGLDIGYEAPRIVNAYIEITPTDNV
                     KYEVDKNTGFLKVDRPQAFSSQCPSLYGLIPRTYCGPRVSAYFGQKAGLDKIEGDTDP
                     LDICVLSERPIFKSDILVEAKPIGGLRMIDNGEADDKIIAVLKSDLVYGEWNDISDCP
                     HNLVDRLRHYFLTYKDFPTTHKGQTDIVTTYGREEAYEVIRLSQEDYLEKFRELGRFM
                     EKIFPE"
     gene            1153..1229
                     /locus_tag="VFR02_00245"
     tRNA            1153..1229
                     /locus_tag="VFR02_00245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1187..1189,aa:Val,seq:gac)
     gene            1265..>3001
                     /gene="thrS"
                     /locus_tag="VFR02_00250"
     CDS             1265..>3001
                     /gene="thrS"
                     /locus_tag="VFR02_00250"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9868916.1"
                     /translation="MSDKHEPKHKMDPELFRVRHSMAHVLAEAVLQVRPKAQLGFGPP
                     IRTGFYYDFLLEEPLTESDFPDLEKRMRRIIQEKQEFKREDLPKDEALKKLEGMGQAL
                     KVEYAKELLAAGEGLSFYSSGPFTDMCEGPHVADTSKIPLMAFKLDSIAGSYWRGDST
                     RPMLTRIYGLCFPTEAELKQFVKDRELAMKRDHRKLGQELELFTIDERVGKGLPLWLP
                     NGTVIREELEKFAKEMEFRDGYVRVATPHIANGELYKLSGHLKLYKEGMFPPMRSVEK
                     DPDSPPEEFYLKPMNCPHHHMVYLARPRSYRELPLRLAEYGTVYRFEKSGQLAGLLRV
                     RGLAMNDAHIYCTEDQIKAEIKKVVAMHQMYFDKFRMDKIWVRLSLHDKDEDKVTENE
                     ALWTKTEGILRDVLRELGVEYEEVTGEAAFYGPKIDYQTENVLGREETAATVQLDFTS
                     PERFELEYTASDGSKQKPFIIHRAPLGTHERFISFLIERWGGAFPTWMSPIQVRVVPV
                     AEDFLPYAEKIKAELDGKLIRAEVDLSNDSFSKKVRNAITRKIPNIWIVGANEVKDST
                     VTWRRYAVEKQVQ"
BASE COUNT          608 a          976 c          904 g          513 t
ORIGIN      
        1 ggcgagatct acgacgtggc cctggacggg cggccggact cgccgacctg tgggcgctgg
       61 gccgcgttcc aactgaagga ctcggggttc caactgctct acatcccccc ggggttcttc
      121 cacggcttct gcgtgctgag cggcccggcg gacgtggcct acaagtgcac ggactactac
      181 gaccggacgg acgaagtcgg ggtcttgtgg aacgatccgg cgctgggcat cgcctggccc
      241 ctggccgacc cggtgctgtc cgagaaggac cggggcctgc cgcccttcgc gcgtgtcctg
      301 cccacgatgg cccccggccc gggcaaataa aactggacag gcggccgggg tgcggtacaa
      361 tctgcacctt tcccaaggcc taggagggct tcgtggcgga ccattccttc gacaaatacg
      421 cgcggcttct cttcaagcac catccctggc acggcttgga catcggctac gaggccccgc
      481 gcatcgtgaa cgcctacatc gagatcaccc ccacggacaa cgtgaagtac gaggtggaca
      541 agaacacggg gttcctcaag gtggaccggc cccaggcctt ttccagccag tgcccgtccc
      601 tctacggcct catcccccgc acctattgcg gcccccgggt gtcggcttat ttcggccaaa
      661 aggccggcct cgacaagatc gagggggaca cggaccccct ggacatctgc gtgctgtccg
      721 aacgccccat cttcaagagc gacatcctgg tggaggccaa gcccatcggg ggactgcgca
      781 tgatcgacaa cggggaagcg gacgacaaga tcatcgccgt cctgaagagc gacctggtgt
      841 acggcgaatg gaacgacatc agcgactgcc cgcacaacct ggtggaccgc ctgcggcact
      901 atttcctgac ctataaggat ttccccacca cccacaaggg ccagaccgac atcgtcacca
      961 cctacggccg cgaggaggcc tacgaggtca tccgcctcag ccaagaggac tacctggaga
     1021 agttcaggga attgggccgc ttcatggaga agatcttccc cgaataggcc catccccttg
     1081 ccttgacagg cggtgggggg acccctacaa ttcggtctgc cgttcgcttc ttttctttgt
     1141 tccggcttcc gggggcgtat agctcagttg gttagagtac ttccttgaca tggaaggggt
     1201 cactggttcg agtccagtta cgcccaccat tttcccggca atccaccgcc gttttggaac
     1261 catcatgtcc gataagcacg aacccaagca taagatggac cccgagttgt tccgtgtgcg
     1321 ccattccatg gcccacgtgc tggctgaggc ggtcctgcag gtgcgcccca aggcccaatt
     1381 gggattcggc cccccgatcc gcacgggctt ttactatgat ttcctccttg aagaacccct
     1441 gaccgagtcc gatttccccg acctggaaaa acgcatgcgc aggatcatcc aggaaaagca
     1501 ggaattcaag cgcgaggacc tgcccaagga cgaggccctg aagaagctgg agggcatggg
     1561 ccaagccctc aaggtcgagt acgccaagga actgctggcc gcgggcgagg ggctgagctt
     1621 ttattccagc gggcccttca ccgacatgtg cgaaggcccc cacgtggccg acacctccaa
     1681 gatccccctc atggccttca agctggattc catcgcgggc agctattggc ggggggattc
     1741 cacccggccc atgctgaccc gaatctacgg cctttgtttc cccacggaag ccgaattgaa
     1801 gcagttcgtc aaggacaggg aactggccat gaagcgggac caccgcaagc tgggccagga
     1861 actggagctc ttcaccatcg acgaacgggt gggcaagggc ctgccgctct ggctgcccaa
     1921 cggcacggtc atccgcgagg aactggagaa gttcgccaag gaaatggagt tccgggatgg
     1981 ctacgtgcgc gtggccacgc cccacatcgc caacggcgag ctgtacaagc tctcgggcca
     2041 tttgaagctg tataaggaag gcatgttccc gcccatgcgc agcgtggaaa aggacccgga
     2101 ttcgcccccc gaagagttct acctgaagcc catgaactgc ccgcaccacc acatggtcta
     2161 cctggcccgc ccccgcagct accgggaact gcccctgcgc ctggccgagt atgggacggt
     2221 gtaccgtttc gagaagtcgg gccagttggc ggggttgctc cgggtgcgcg gcctggccat
     2281 gaacgacgcc cacatctact gcaccgagga ccagatcaag gccgagatca agaaggtcgt
     2341 ggccatgcac cagatgtatt tcgataagtt ccgcatggac aagatctggg tgcggctttc
     2401 cctgcacgac aaggacgagg acaaagtcac cgagaacgag gccctctgga ccaagaccga
     2461 gggcatcctg cgggacgtgc tgcgggagtt gggggtggaa tacgaggaag tgaccgggga
     2521 agcggccttc tacggcccca agatcgacta ccagaccgag aacgtgctgg gccgggaaga
     2581 gaccgccgcc accgtgcagt tggacttcac ctcgcccgag cgcttcgagc tcgaatacac
     2641 ggcctccgac gggtccaagc agaagccctt catcatccac cgggcgcccc tgggcaccca
     2701 cgagcggttc atctccttcc tcatcgaacg ctggggcggc gccttcccga cctggatgtc
     2761 gcccatccag gtgcgcgtgg tgccggtcgc cgaggacttt ttgccctacg ccgagaagat
     2821 caaggccgag ctggacggga agctcatccg cgccgaggtc gacctctcca acgactcctt
     2881 ctccaaaaag gtgcgcaacg ccatcacccg caagatcccc aatatctgga tcgtgggcgc
     2941 caacgaggtc aaggactcga ccgtcacctg gcgccgctac gcggtggaga agcaggtcca
     3001 a
//
LOCUS       DASTJJ010000025         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1716615, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000025 DASTJJ010000000
VERSION     DASTJJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716615"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..>1407)
                     /locus_tag="VFR02_00255"
     CDS             complement(198..>1407)
                     /locus_tag="VFR02_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868917.1"
                     /translation="AAYEVLLAQSRKPWVSLLGPVFLSLTPRLVGALPADPKDGPFAV
                     LYFTSLTAVFLMARRPISPWFKVPALGLLFGLTTAQRLVGFTLFLVWPLYEWILWRWG
                     LKPKARRKGSGARPILIYAGVFFISQLILAATWPYLAADYFHRLPELLKAAKDYPWND
                     PVLFLGREIPASGLPRTYLPVFLLVTTPLLALFWFAASFRALRKPQADPLWALLGLAL
                     GLNLALYLILKPVVYDGLRHFLFLLPLTAVMAACGLANLLARPRAPGRRPLLALSALL
                     GILALADMARLHPYEYVYFNELTGGLKGAQGRFETDYWGASYKEAVDWVQAQEPPGDT
                     PLWVHSNGNPYQVACYLNDRTHWTDDLGKADYYLATTRDGANLSVDPARRVHRVEREG
                     VPLCDVFKLK"
BASE COUNT          297 a          420 c          490 g          200 t
ORIGIN      
        1 gctggaccgg gccgcggcgg gcatcgagac ctatatcctt cagggcgcgg acgccgccat
       61 gcgggaactc aacggggata tttaaaaccc ccctttacca cagaggcgcg gagacgcagt
      121 aaggcgaaaa tggttgggtt gagatgataa aatcgatttt ccgcgtctct gcgtccctgc
      181 ggtgaaaaag atgtgactta cttcaacttg aacacgtcgc acaggggcac gccctcgcgt
      241 tccacccggt ggacccgccg ggcggggtcc accgaaagat tggcgccgtc ccgggtggtg
      301 gccaggtaat agtcggcctt tcccaggtcg tccgtccaat gggtccggtc gttgaggtag
      361 caggccacct gataggggtt gccgttggaa tgcacccaga ggggtgtgtc cccgggcggt
      421 tcctgggcct gcacccagtc caccgcttcc ttgtagctgg ccccccaata gtcggtctca
      481 aagcgcccct gggcgccctt cagccccccg gtcaactcgt tgaaataaac atattcataa
      541 gggtgcagcc gcgccatgtc cgccagcgcc aggatgccca agagggcgct gagggccagc
      601 aggggcctgc gcccgggcgc gcgggggcgg gccagcaggt tcgccaggcc gcaggcggcc
      661 atcacggccg tcaggggcag caggaacagg aagtgccgca ggccgtcata gaccacgggt
      721 ttcaggatga gataaagggc caagttgagc cccagggcca ggcccaggag cgcccacagg
      781 gggtcggcct ggggcttgcg cagggcccgg aaggaggccg cgaaccaaaa gagggccagg
      841 aggggcgtgg tcacgagcag gaagaccggc aggtaggtcc ggggtagccc ggacgcgggg
      901 atctcccggc ccaggaacag gaccgggtca ttccagggat aatccttggc ggccttcagc
      961 agttccggca gccggtggaa ataatccgcc gccaggtagg gccaggtggc cgccaggatc
     1021 aattgggaga tgaaaaagac cccggcatag atcaggatcg gccgggcgcc cgatcccttc
     1081 cgccgcgcct tgggctttaa cccccaacgc caaaggatcc attcataaag gggccagacc
     1141 aggaacagcg tgaagcccac cagccgctgg gcggtcgtga gcccgaacag caatcccagg
     1201 gcggggacct tgaaccaggg ggaaatgggc ctgcgggcca ttaaaaagac ggcggtcagg
     1261 ctggtgaaat aaagcacggc gaaggggccg tccttgggat cggccggcag ggcgccgacg
     1321 agccgaggcg tgagtgaaag gaaaaccggc cccaggaggg aaacccaagg cttccgggat
     1381 tgggccaaca aaacttcata ggcggcg
//
LOCUS       DASTJJ010000026         1971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_126653, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000026 DASTJJ010000000
VERSION     DASTJJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126653"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1117)
                     /locus_tag="VFR02_00260"
     CDS             complement(77..1117)
                     /locus_tag="VFR02_00260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9868918.1"
                     /translation="MDIFLLRLDRIGDFILGIPAYRALREAHPHDRISVVVPSAAAEL
                     ARSCPYFDEVYVFEARWLMPGEKPRVRWRSAWQLIHFLRPKRVDLLINFRYQNRLDPL
                     VTGLSGAKKSVGFDHGFWSRFLTTAVPQPLPGTHQVRRNLLPLEALGIRTHDLSLGMW
                     WDERDEKRALEHLPRQESLPGLPRLGIHLGAATPAKRWGEEDFAALIHELQAATEGEI
                     LVFGGEEDLPFAHEVLDGLKAPVVNLVGKLTLRQMASLIAGCRAFVGCDTGPTHLAAA
                     VGTPVVSLFSAANEAAVWKPWGEKVKVLTRQPSCSPCQSYACRRTDGYFCMREIKVEE
                     VVEAVKGFLAPR"
     gene            1243..>1971
                     /locus_tag="VFR02_00265"
     CDS             1243..>1971
                     /locus_tag="VFR02_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9868919.1"
                     /translation="MKNTATGLCLGLALCLSLACSKKQADTEIPVGTPTPTTQAPGPA
                     APVTLPPRPAAFGPKTEMAFAMPSPTTTPQPLEYEVDDIQGTVLALFSGASAPETVAE
                     GETLQSGDTLMTGSGSQATLTLNDVTAVQVSASTTVKVADLEPNATQGFTSRLQLAGG
                     RVLSEVQKLTESHSTFEVEANGVVCGVRGTAFEVTDQDGQVDTDTFHGSVEVQRDKLI
                     QLVAGGEHSSFSVKRRAFLAKRRLN"
BASE COUNT          345 a          687 c          640 g          299 t
ORIGIN      
        1 ggacgtggaa tcgggcaagt ggcgcttctt gacgaaggaa gaggcctttt tgctgcacgc
       61 gcatttgaag tgatccctac ctcggcgcca ggaacccctt caccgcctcc acgacttcct
      121 cgaccttgat ctcccgcatg cagaaatagc cgtcggtgcg ccggcaggcg tagctttggc
      181 agggcgaaca ggaaggctgc cgggtcagga ccttcacctt ttccccccag ggcttccaga
      241 ccgccgcctc gttggcggcc gagaagaggc tgaccacggg cgtgccgacg gccgccgcca
      301 ggtgggtggg cccggtgtcg cagcccacga aggcccggca gccggcgatg agcgacgcca
      361 tttggcgcag ggtgagcttg cccaccaggt tcaccacggg ggccttgagg ccgtccagca
      421 cctcgtgggc gaagggcagg tcctcctcgc cgccgaagac caagatctcc ccctcggtgg
      481 cggcctgcag ttcatggatc agggcggcaa aatcctcctc accccaacgt ttggcgggcg
      541 tggccgcccc caggtggatg cccaacctgg gcaggccggg caggctttcc tgccggggca
      601 ggtgttccag ggcccttttc tcgtcccgct cgtcccacca catgcccaac gacaggtcgt
      661 gcgtccggat gcccagggct tccaggggca ggaggttgcg ccggacttga tgcgtcccgg
      721 ggagcggctg ggggacggcc gtggtcagga aacggctcca gaacccatgg tcgaacccca
      781 cgctcttttt ggcgcccgac aggccggtga ccagggggtc caaccggttc tggtaacgga
      841 aattgatcaa gaggtcgacc cgcttgggcc ggaggaaatg gatgagctgc caggccgatc
      901 tccaacgcac ccggggcttt tccccgggca tgagccaccg ggcctcgaaa acgtagacct
      961 cgtcgaagta ggggcaggag cgggccaatt cggccgccgc cgagggcacc accaccgaga
     1021 tcctgtcgtg gggatgggcc tcccgcaggg cccggtaggc cgggatcccc aagataaaat
     1081 cgccgatccg atccagccgc agcaggaaaa tgtccatggc cgcatggtaa cggaaccggc
     1141 cggaccccgc cactggtttg gcccgccggg aaccgcaact ttagcgcccc gaccccgccg
     1201 aactgctata gaattggccc gaaatcccct tcggaggccc gcatgaaaaa caccgccacc
     1261 ggtctctgcc ttggcctcgc cctgtgcctg tccctggcct gctcgaaaaa acaggccgac
     1321 accgagatcc cggtgggcac ccccaccccc accacccagg cgcccggacc cgccgcgccc
     1381 gtcacgctgc cgccccgccc cgccgccttc gggcccaaga ccgagatggc cttcgccatg
     1441 ccctccccca ccacgacccc gcaacctctg gaatatgagg tggacgacat ccaaggcacc
     1501 gtgctggcgc tcttcagcgg cgcgtccgcc ccggagaccg tggccgaggg ggagaccctg
     1561 cagtccggcg ataccctgat gaccgggtcg gggtcgcagg ccaccctcac cttgaacgac
     1621 gtcaccgccg tccaggtctc ggcgtccacg acggtgaagg tggccgacct ggagccgaac
     1681 gccacccagg ggttcaccag ccgcctgcaa ttggccggcg gtcgggtctt gtcggaggtg
     1741 cagaaattga cggagagcca ttccaccttc gaggtggagg ccaacggggt ggtctgcggc
     1801 gtgcggggga cggccttcga agtgaccgac caggacggcc aggtggacac cgacaccttc
     1861 cacggctcgg tcgaggtgca gcgggacaag ctgatccaac tggtggcggg cggcgaacat
     1921 tcgtcctttt cggtcaaacg ccgggctttc ctggccaagc gcaggttgaa c
//
LOCUS       DASTJJ010000027         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_371928, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000027 DASTJJ010000000
VERSION     DASTJJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371928"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFR02_00270"
     CDS             complement(<1..786)
                     /locus_tag="VFR02_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868920.1"
                     /translation="MIFFLTTRFGRHTVDEYLAWKGAPAREFLKPVTYAQAARTWGKT
                     DPPPGVYIFADLERLSPEAQQGAERLWERLQKDPERWLTVNRPGGTLRRLELLRRLHQ
                     AGINRHRAWPLSEAPPPDVRFPVFLRVADDHRGARTGLLRDRGELEGAAAALRRKGTD
                     LSGWIGCEYAGTVRPDGLHAKYAAFRVEGDIYPRGILFAARWQVKYPERFGEGLLTEE
                     WDYGRTNPHADTLRRVFELAGADFGRVDYGLDPEGRVSVWEINT"
     gene            complement(783..1196)
                     /locus_tag="VFR02_00275"
     CDS             complement(783..1196)
                     /locus_tag="VFR02_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9868921.1"
                     /translation="MNELSVARGWLDRFQDCVRGLDYAGARALFDPDVKAYGSLAGNL
                     EGLQALEARQWRRVWPWIRDFTFQADKMRLEGTVPSGVLTLALPWSSRGVSAGDPAFD
                     RPGRATLVLRLGGDGAWRCVHSHYSLLPVDPGAPR"
     gene            1341..>2237
                     /locus_tag="VFR02_00280"
     CDS             1341..>2237
                     /locus_tag="VFR02_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HET9868922.1"
                     /translation="MKLLSPFSLGPLSLPNRITMSAMTRCRALAGNVPNPLAAVYYSQ
                     RASAGLIVTEATQVTPMGQGYPNTPGIHSDAQVEGWKKITDAVHQAGGRIFLQLWHVG
                     RVSLPDYHGGELPVSASALPVKGHLHTPVFEQKDIVTPRALETAEIAGVVAEFKKGAQ
                     NAKAAGFDGVEIHGANGYLLEQFTKSGSNHRTDAYGGSLANRLRFPLEVVDAVVSVWG
                     PERVGYRVSPHFAGYSMSDDNPVETYTALVKELNKRKLGYLCVKEGPKDAKTLPAGTP
                     LLTPLLRPLFHQAYMVNQGFDRE"
BASE COUNT          396 a          798 c          722 g          321 t
ORIGIN      
        1 ggtgttgatc tcccagacgc tcacccggcc ctccggatcc aggccgtaat ccacccgccc
       61 aaaatccgcc cccgccagtt caaagacccg ccgcagggtg tccgcgtggg ggttcgtccg
      121 gccgtaatcc cattcttccg tcaacagccc ctcgccgaag cgctcgggat acttgacctg
      181 ccagcgggcc gcgaaaagga tgccgcgcgg ataaatgtcc ccctccaccc ggaaggccgc
      241 gtatttggcg tggaggccgt cggggcggac ggtccccgcg tactcgcaac cgatccaacc
      301 cgaaaggtcc gtccccttgc ggcgcagggc cgcggcggcc ccttccaatt ccccccggtc
      361 ccgcaacagc ccggtgcggg cgccccggtg gtcgtcggcc acgcgcaaaa aaaccgggaa
      421 ccggacatcc ggaggcggcg cctccgaaag gggccaggcg cggtgccggt tgatgcccgc
      481 ctggtggagc cggcgcagca gttcgagccg gcgcagggtc ccgccgggac ggttcaccgt
      541 cagccagcgc tccgggtcct tttgcaggcg ctcccaaagg cgctcggccc cctgctgcgc
      601 ctccggggac aaccgctcga ggtcggcgaa gatatagacc ccggggggag ggtcggtttt
      661 cccccaggtc cgggccgcct gggcgtaggt gaccggcttc aaaaattccc gggccggggc
      721 gcctttccag gccaagtatt cgtccaccgt gtggcggccg aatcgggtgg tcaggaaaaa
      781 gatcaccgtg gggcgcccgg atcgacgggg agcagggaat aatggctgtg cacgcagcgc
      841 caggccccgt ccccgcccaa gcgcagcacc agggtggccc ggcccggacg gtcaaaagcc
      901 gggtcgcccg cggagacgcc ccgggaggac cagggaaggg ccagggtcag gacgccggag
      961 gggaccgtcc cctccaaacg catcttgtcc gcctggaagg tgaagtcccg gatccagggc
     1021 cagacccggc gccattgccg ggcttccaac gcctgcaacc cctccaggtt cccggccagg
     1081 cttccatagg ccttgacgtc gggatcgaag agggcccggg cgcccgcata atccaggccc
     1141 cgcacgcagt cctggaaacg gtccaaccag ccgcgggcga cggaaagttc gttcatagcc
     1201 ctgagattgt gcggacggcg ggccaaatag caacaggatt cgccggtcac cccggggtaa
     1261 cccgatttcc ggccgggagg ctaccaaacc accgcccgga tgggttataa aacggccaat
     1321 ccaagcacaa aggaaccgcc atgaaactgc tctcgccctt ttcgttgggc ccgctgtccc
     1381 tgccgaaccg catcaccatg tccgccatga cccgctgccg ggcgctcgcc ggcaacgtgc
     1441 ccaatcccct ggcggccgtc tattattccc aacgcgcctc cgccgggctc atcgtgaccg
     1501 aagccaccca ggtcaccccc atgggccagg gctaccccaa caccccgggc attcactcgg
     1561 acgcccaggt ggaaggatgg aagaagatca ccgacgccgt ccaccaagcg ggcggccgca
     1621 tctttttaca gctctggcac gtgggccggg tgtcgttgcc cgactaccac gggggcgagc
     1681 tcccggtctc ggcctcggcc ctgcccgtga agggccacct gcataccccg gttttcgagc
     1741 agaaggacat cgtcaccccc cgggccttgg agacggccga gatcgcgggg gtcgtggcgg
     1801 agttcaagaa gggcgcccag aacgccaagg ccgcgggctt tgacggggtc gagatccacg
     1861 gcgccaacgg ctacctgctg gagcaattca ccaagagcgg atccaaccac cgcaccgacg
     1921 cctacggcgg gagcctggcc aaccgcctgc gtttcccgct ggaagtggtg gacgccgtgg
     1981 tctcggtctg gggcccggag cgcgtggggt accgcgtctc cccccatttc gcgggttatt
     2041 cgatgtcgga cgacaacccg gtggagacct acacggccct ggtgaaggaa ctcaacaagc
     2101 gtaagctcgg atacttgtgc gtgaaggaag gccccaagga cgccaagacc ctgccggccg
     2161 gcacgcccct cttgacgccc ctgctgcggc cccttttcca ccaggcctac atggtgaacc
     2221 aggggttcga ccgggag
//
LOCUS       DASTJJ010000028         1348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_372156, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000028 DASTJJ010000000
VERSION     DASTJJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372156"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="rph"
                     /locus_tag="VFR02_00285"
     CDS             complement(<1..297)
                     /gene="rph"
                     /locus_tag="VFR02_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010642979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET9868923.1"
                     /translation="MPPSIRPIRFTRSFTRYAEGSVLACMGQTRVLCTASVAEEVPAF
                     LRGKGKGWVTAEYSMLPRSTQDRSPREAAKGKLGGRTHEIQRLIGRSLRAVMDRE"
     gene            complement(335..1264)
                     /locus_tag="VFR02_00290"
     CDS             complement(335..1264)
                     /locus_tag="VFR02_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868924.1"
                     /translation="MRKSDDELKIDITELFGGDLEPARQAAEQSLEEAAPAPPAGEAA
                     PALEAAPAPPDQQYQEWISARERELETKTQELEKRLQDLLEQKLQAAQAAAPAPVPEA
                     PPAEEAPASPAESPAPAGEGLAIDFSQPIRPPFLPDAVPPAPAEAALGSPVSDEEGLK
                     RAQREYEFLLLYDEFRGIILHELRDLVGERKTFKMLERTVELAREKYPEIFRNANWDA
                     AGNLLEDGSLDSQRLVENKASLDPAKANQVFDAGLGALLSLRLQAVEKGLGTGMKNKV
                     RARLYQWINEKAERAAQAGKAVDDLNRLKAFVV"
BASE COUNT          176 a          461 c          472 g          239 t
ORIGIN      
        1 ctcccggtcc atcacggccc gcagggaccg gccgatgagc cgttggatct cgtgggtccg
       61 cccgcccagc ttgcccttgg cggcttcccg ggggctgcgg tcctgggtgc tgcggggcaa
      121 catggaatat tcggccgtga cccagccctt gcccttcccc ctcaggaaag ccgggacctc
      181 ctcggccacg gacgccgtgc aaagcacccg ggtctggccc atgcaggcca agaccgagcc
      241 ttcggcgtag cgggtgaagg accgggtgaa gcggatggga cggatggaag gcggcatggg
      301 ggctcctcca ggcatgggtt gggacggcgg cgggtcagac gacgaaggcc ttcaaccggt
      361 tcaggtcgtc cacggctttt ccggcctggg ccgcccgttc ggccttttca ttgatccact
      421 ggtacaaccg ggcccggacc ttgttcttca tccccgtgcc caggcccttc tccacggcct
      481 ggagccgcag ggacaggagg gcccccagtc cggcgtcgaa gacctggttg gccttggcgg
      541 gatccaagga ggccttgttc tccaccagcc gctggctgtc caaggaccca tcctccagca
      601 ggttgccggc ggcgtcccag ttggcgttgc ggaagatctc cgggtacttt tcccgggcca
      661 attccaccgt gcgttcgagc atcttgaagg tcttgcgctc gcccaccagg tcgcgcagct
      721 catgcaggat gatgccccgg aattcgtcgt acaacaggag gaactcgtat tcccgctggg
      781 cccgtttgag gccttcttcg tcgctcacgg gggatcccaa cgccgcctcc gccggggccg
      841 gcggcacggc gtcgggcagg aagggggggc ggatgggctg gctgaagtcg atggccaggc
      901 cctccccggc gggggcggga gactcggccg gggaggccgg ggcctcctcg gcggggggcg
      961 cctcgggtac gggagcgggg gcggccgcct gggcggcctg gagcttttgt tccagcaggt
     1021 cctggagccg tttctccaac tcctgggtct tggtctccaa ctcccgttcc cgggcgctga
     1081 tccattcctg gtactgctgg tccggcggcg ctggggcggc ttccagcgcc ggggccgctt
     1141 ccccggcggg cggcgccggg gcggcctctt ccaggctctg ctcggccgcc tgccgggcgg
     1201 gctccagatc gcccccgaac agctcggtga tgtcgatttt cagctcatcg tcggacttgc
     1261 gcatcaggcc accttccatg ggaaagccgc tgcatcatat cggcttccgg cgaaacttgt
     1321 caacgaaccg cccccgggcc cgggccgg
//
LOCUS       DASTJJ010000029         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_4619, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000029 DASTJJ010000000
VERSION     DASTJJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4619"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFR02_00295"
     CDS             complement(<1..253)
                     /locus_tag="VFR02_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9868925.1"
                     /translation="MRIEPLLPRKRARLEIIPLIDIMFFLLATFIMVSLSMIQNRGLV
                     VALPAASAAVAQAAPSVVLGLTAQGALYWDKQPIGAADLP"
     gene            complement(250..858)
                     /locus_tag="VFR02_00300"
     CDS             complement(250..858)
                     /locus_tag="VFR02_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9868926.1"
                     /translation="MFDLFLKGGPMMWPLLLLSILVLAVVLERLWFLWREGGMRDPRT
                     TDRFWEMLSTRRPDEAMVTSLRSPDFVLRTLAYALEHRDRGLAAALSQAAHRELKRFS
                     RGLVLLDTSITLAPLLGLLGTVVGLIRSFGLLGEDQLQSPTVLTGGIAQALLATAFGL
                     CVAILALLPFNYLQARVEEARHEIEDQATRLELALGRTGPLP"
     gene            952..1200
                     /locus_tag="VFR02_00305"
     CDS             952..1200
                     /locus_tag="VFR02_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868927.1"
                     /translation="METLPSGTPLDPDADPSLAPGNPAPHMNIDISARRPGEPLRYTG
                     PHLCVTCAAQDRTVSVYLRAGDPAPDCPHCGPRALWEP"
     gene            1279..2223
                     /locus_tag="VFR02_00310"
     CDS             1279..2223
                     /locus_tag="VFR02_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9868928.1"
                     /translation="MAHDFTITFRGVRGSLPVPGPSTLKIGGNSSCVQVNVLDHTIFL
                     DAGTGIVPAGQQIARNFFANKDKVKRLSTTVLISHTHHDHIMGLPFFPPLYLPDSALY
                     IYGPSIYGPVQHAMETNLEHVLEMMMDQMFFPIDLRETHSIKSIHTLKPFDQVFLAEG
                     VPEPELSNLQSDAKLSFKDKVIISQYRSYSHPKNGTLIYKLQYAGKSIVYATDIEGYV
                     DGDRRLINFAKGADLLIHDTQYFADQYAGAGMPLQGFGHSTVEMATQVAREAGVKQLV
                     LYHHDPNHDDAAIAKKEEAAKKLFTNSIAAYEGLVIPV"
     gene            complement(2339..3022)
                     /locus_tag="VFR02_00315"
     CDS             complement(2339..3022)
                     /locus_tag="VFR02_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868929.1"
                     /translation="MADDFSAEQLAESMAGWKPAPDLAEKLAARLKRGGVGVETQKPL
                     AAGLAQCARSTFDYIQFIHEFSTVKELDAKGLAPQMAHWGFLVGDLRSQAKAYGEAFQ
                     RYSEAAQPGMTRDHFDTHYYDAILGLDIRGLLKGLLGEVEVLLQAMALASGELKGLDG
                     LGVYEDCVRFQLFLQYLLLRDKFNQEEMWSVLFDLYNLLEGLGKPSVEPSPEDLQDLQ
                     GRVRNFKAN"
     gene            complement(3024..3887)
                     /locus_tag="VFR02_00320"
     CDS             complement(3024..3887)
                     /locus_tag="VFR02_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9868930.1"
                     /translation="MSKVADLHLHTTHSDGTLTPRELARLAKAQGVDAIALTDHDTVS
                     GIEELLDEGRILGLEVIPGVELSADFGPGTLHILGYGFDPKGPIGGRLKRFQKARAER
                     NPQVLERLRELHMPLTLEEVKALSGATGQLGRPHIARALERKGYVRSYEEAFDRYLSK
                     GKPAYVPKATLAAEECVKLIHESGGVAVAAHPVQMRLTGAALRDKLSELAALGMDGLE
                     VIHPDHGSEAQTLFSDLADALGLLKTGGSDFHGDHKPGVQLGQGRPPYLFLEKLRNRI
                     SERRRRYALRN"
     gene            complement(3895..>5287)
                     /locus_tag="VFR02_00325"
     CDS             complement(3895..>5287)
                     /locus_tag="VFR02_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868931.1"
                     /translation="GGGMYWEGSLFAGVTGMTLALFGLFASRAPRRLTAAGLGLFLLL
                     LAMGKRTPLFMVFYHAVPFFGDFRGAAKLNILVSLWVCYLAAAGLDHYLGQEGPVRRL
                     VRWAGGFALGVSALAAFFHFAPALGWGRLYGKFGAHAPGMVESLLLCGLTLGAAALAA
                     WAGKRFPRWRLSLLGLAFLELLLFARANRGGFDLPALQAKAAAIQAVYDQDPGDYRVT
                     AEPSNITLGTSGFDVWGNDPMIPGRYALFLAESQHLDVGDHFVDKPFFRSYPPALGLT
                     RLRYVFEQDGEGWRVRRLALREQPRALLVGGWREEQGETAVQTLLRPGFNPRREALVE
                     SDPGFPSRPGPGKGSVRLRDLSTDALEVRADNEGPCLLVVGDNDAPGWRAVAAPDDPG
                     SYRVVPVNFFQRGVPLAPGSHHFTLEYRPPFLTAAAWVSAVSLCLTLAGAAVGWRRRA
                     LLPYDESQSLRKP"
BASE COUNT          940 a         1847 c         1714 g          786 t
ORIGIN      
        1 ccggcaggtc cgccgcgccg atgggctgtt tgtcccaata gagcgcgcct tgggccgtca
       61 gccccagcac caccgagggg gcggcctggg ccacggcggc cgaggcggcg gggagcgcca
      121 ccaccaggcc ccggttctgg atcatggaca gcgacaccat gatgaaggtg gccaggagga
      181 agaacatgat gtcgatgagc gggatgatct ccagcctggc ccgcttgcgg ggcaaaaggg
      241 gctcgatcct cacggcaggg ggcccgtgcg gccgagcgcc agttccaggc gggtggcctg
      301 gtcctcgatc tcgtggcggg cctcctccac gcgggcctgc aggtagttga agggcagcag
      361 cgccaggatg gccacgcaga ggccgaaggc cgtggccagg agcgcctggg cgatgccgcc
      421 ggtgaggacg gtgggcgact gcagttggtc ctcgcccagg aggccgaagg aacggatgag
      481 ccccaccacc gtgcccaaca gccccagcag gggcgccagg gtgatgctgg tgtccaacaa
      541 caccaacccc cgggaaaagc gcttgagctc ccggtgggcg gcctgggaca gggcggcggc
      601 caggccccgg tcccggtgct ccagggcgta ggccagggtg cgcagcacga agtcgggcga
      661 gcgcaggctc gtcaccatgg cctcgtccgg gcggcgggtg gacagcatct cccagaagcg
      721 gtcggtcgtc cgggggtcgc gcatgccccc ctcgcgccag aggaaccaga ggcgctccag
      781 caccaccgcc aggaccagga tggacaagag caggagcggc cacatcatgg ggccgccctt
      841 caggaacagg tcgaacatgg gtctccttca ccgaggggga tgcaggattc taccccggcg
      901 cgcggggcgg attcatttga aaagctccgt caaatgccgt aaaaagggtc catggaaacg
      961 ctcccctccg gaacgcccct ggaccccgac gccgaccctt ccttggcgcc cgggaacccc
     1021 gccccccaca tgaacatcga catctcggcc cggcggcccg gcgagcccct gcgctatacc
     1081 ggcccccacc tctgcgtgac ctgcgcggcg caggaccgga cggtcagcgt ctatctgcgg
     1141 gccggcgacc cggcccccga ctgcccccac tgcgggcccc gcgccctctg ggagccctga
     1201 tccgggtttc tccgttgaaa aaggccgctt ttgtgaaata atgtcggcaa cgccggtacc
     1261 caatccgaac ggagccccat ggcacacgac tttaccatca ctttccgagg ggtccggggc
     1321 agcctccccg ttccggggcc ctccacgctg aagatcggcg gcaactcctc ctgtgtgcag
     1381 gtcaacgtcc tggaccacac catcttcctg gacgccggca cgggcatcgt gcccgcgggc
     1441 cagcagatcg ccaggaactt tttcgccaac aaggacaagg tcaagcgcct gtccaccacg
     1501 gtcctcattt cccacacgca ccacgaccac atcatggggc tgcccttctt cccgcccctc
     1561 tacctgcccg attcggccct ctatatctac ggcccctcca tctacgggcc cgtgcagcac
     1621 gccatggaga ccaacctgga gcacgtgctg gagatgatga tggaccagat gttcttcccc
     1681 atcgacctgc gggagaccca ctcgatcaag agcatccaca ccctcaagcc cttcgaccag
     1741 gtcttcctgg ccgaaggcgt gcccgagccc gagctgtcca acctgcagtc cgacgccaag
     1801 ctgtccttca aggacaaggt gatcatcagc cagtaccgca gctattccca ccccaagaac
     1861 ggcaccctca tctataagct gcagtacgcg ggcaagtcca tcgtgtacgc caccgacatc
     1921 gagggttacg tggacggcga ccggcggctc atcaacttcg ccaagggcgc ggaccttctg
     1981 atccacgaca cccagtattt cgccgaccag tacgccgggg cgggcatgcc cctgcagggc
     2041 ttcggccatt ccaccgtcga gatggccacc caggtggccc gggaagcggg cgtcaagcag
     2101 ctggtgctct accaccacga ccccaaccac gacgacgcgg ccatcgccaa gaaggaagag
     2161 gccgccaaga agctcttcac caactccatc gcggcgtatg aaggacttgt cataccggtt
     2221 taaaagttaa aaaaaagtcc ttggccgaga cgcgatgggg cccgcccccc cgtcctgatc
     2281 aaaacaaaaa acccccgccc tttgggaacg ggggttttta ttttccaaac catccggatc
     2341 agttcgcctt gaaattcctc acgcggccct gcaggtcctg gaggtcttcc ggcgagggct
     2401 ccaccgaggg cttgcccagc ccctccaaca ggttgtagag gtcgaacagc acggaccaca
     2461 tctcttcctg gttgaacttg tcccggagca gcaggtattg caggaaaagc tggaaccgga
     2521 cgcagtcctc gtagacgccc aggccgtcca ggcccttcag ttccccgctg gccagggcca
     2581 tggcctgcag cagcacctcc acctcgccca agagcccctt cagcaggccc cggatgtcca
     2641 gccccaggat ggcgtcgtaa tagtgggtgt cgaaatggtc gcgggtcatc ccaggctggg
     2701 ccgcctcgct gtagcgctgg aaggcctccc cataagcctt ggcctgggag cgcaggtccc
     2761 ccaccaggaa gccccaatgg gccatctggg gggccaggcc tttggcgtcc agttccttga
     2821 ccgtggagaa ctcatggatg aactggatgt agtcgaaggt ggaccgggcg cattgggcca
     2881 atccggcggc caggggcttt tgcgtttcca cgcccacccc gccgcgcttc aaccgcgccg
     2941 cgagcttttc ggccaggtcg ggcgcgggct tccagcccgc catgctctcg gccagttgtt
     3001 cggcgctgaa gtcgtcggcc atgtcagttc ctcaaggcgt agcgccggcg gcgctcgctg
     3061 atgcggttgc gcagcttttc caagaacagg tagggcgggc ggccctggcc cagctgcacg
     3121 ccgggcttgt ggtccccgtg gaaatcgctg ccgccggtct tgagcagtcc cagggcgtcc
     3181 gccaggtcgg aaaagagggt ctgggcctcg gacccatggt cggggtggat cacttccagg
     3241 ccgtccatcc ccagggccgc cagctccgac aacttgtccc gcagggccgc gcccgtcagg
     3301 cgcatctgca ccggatgggc ggccacggcc accccgccgg actcatggat gagtttcacg
     3361 cattcctcgg ccgccagggt ggccttgggc acataggcgg gcttgccctt ggacaggtag
     3421 cggtcgaagg cttcctcgta actgcgcacg tagcccttgc gctccagcgc gcgggcgatg
     3481 tggggacggc ccaactgccc ggtggcgccg gacagggcct tcacttcctc cagcgtcagg
     3541 ggcatgtgca gctcgcggag cctttccagc acttgggggt tgcgctccgc ccgcgccttt
     3601 tggaagcgct tgagcctccc cccgatgggg cccttggggt cgaacccata gcccaggata
     3661 tgcagggtgc cggggccgaa gtcggccgac agctccaccc cggggatcac ctccaggccc
     3721 aggatgcggc cttcgtccaa cagttcctcg atgcccgaca cggtgtcgtg gtcggtcagg
     3781 gcgatggcgt ccaccccctg ggccttggcc aggcgggcca gttcgcgggg cgtgagggtc
     3841 ccgtccgaat gggtggtgtg caggtgcagg tcggcgactt tgctcatggg tgcctcaggg
     3901 ctttctcaag gactggcttt catcataagg caaaagggcc cggcggcgcc acccgaccgc
     3961 cgcccccgcc aaggtcaggc ataaggagac cgccgagacc caggcggccg ccgtgaggaa
     4021 ggggggccgg tattccagcg tgaaatggtg tgatccgggc gccaggggca cgccccgttg
     4081 gaagaaattc accggcacca cccgatagct ccccggatcg tccggcgcgg ccaccgcgcg
     4141 ccaaccgggg gcgtcattgt cgcccaccac cagcaggcag ggcccttcgt tgtcggcccg
     4201 cacctccaga gcgtcggtgg aaaggtccct caaccgcacg cttcccttcc cggggccggg
     4261 gcgggagggg aatcctgggt ccgattccac cagggcctcc cggcgggggt tgaagccggg
     4321 ccgaaggagg gtttggaccg ccgtctcgcc ttgttcctcg cgccatcccc ccaccaagag
     4381 ggcgcggggc tgttcacgca aggcgagcct gcgcacccgc cagccttccc cgtcctgctc
     4441 aaaaacgtaa cgcaggcggg tcagtcccaa ggccggggga taggaacgga aaaagggttt
     4501 gtccacgaaa tggtccccca cgtccaggtg ctggctttcc gccaggaaaa gggcgtagcg
     4561 gcccgggatc atggggtcgt tgccccacac gtcgaagccc gaggtgccca gcgtgatgtt
     4621 gctgggctcg gcggtcaccc ggtaatcgcc cggatcctgg tcatagacgg cctggatggc
     4681 ggcggccttc gcctgcagcg ccggcaggtc gaagccgccc cggttggccc gcgcgaagag
     4741 gagcagttcc aggaaagcca gccccagcag cgagagccgc caccggggga agcgcttgcc
     4801 cgcccaggcc gccagggccg ccgcccccag cgtgaggccg cagagcaaaa ggctttccac
     4861 catgcccggc gcgtgggcgc cgaacttgcc gtaaaggcgg ccccagccca aggccggggc
     4921 gaaatgaaag aaggccgcca gggccgagac ccccagggcg aagcccccgg cccagcggac
     4981 caggcgccgg accgggcctt cctgccccag gtaatggtcc aaccccgccg ccgccaggta
     5041 gcagacccag agcgacacca ggatgttcag cttggccgcg ccccggaagt cgccgaagaa
     5101 gggcacggcg tgatagaaga ccatgaagag cggcgtgcgc ttgcccatgg ccaagagcag
     5161 caggaacaac cccagcccgg ccgccgtcag gcgccggggc gcccgggagg cgaagaggcc
     5221 aaagagggcc agcgtcatgc ccgtgacccc cgcgaagagc gaaccttccc aatacatgcc
     5281 gccgccc
//
LOCUS       DASTJJ010000030         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1717534, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000030 DASTJJ010000000
VERSION     DASTJJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717534"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VFR02_00330"
     CDS             complement(<1..1663)
                     /locus_tag="VFR02_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868932.1"
                     /translation="MKPALLRALVLCLAAAPFAPLDAQVPVTITTYAGDTSAGSLGAA
                     VSTSNSTGNQDLVFQISPGGGSVTLGQPLPAFTQAASFTGVDTGSFHGVNLYGNDSSQ
                     STLFFQGSFYEGNKVALNLFDLGSGQDAGLTATNVFMDANSGLGIDAGDGGFLSTSGG
                     SGDTGQAGGKAFWQSAAVTMNGIQGGSLQAGAGGAVTDLGGTNDVGGAGGQASFQTGA
                     WVLSDSNYSIVGGAGGAVSDASGSVGGAGGAVSVSTGNWSVDGSSGVFMNGGNGQAGD
                     SGGMGGSVTVASGVATVTGSNWILTAGKGGSGDTAGGAGGSVLTNWGSMTVSQGAGLN
                     LTAGNGGSASLTGGAGGNLASTGVSILESGSASNFNLKGGNGGNSSAGTGGNGGSAAL
                     TMINVQLTSATGMSLSGGNGGSGVDGGNGGNAFVSIGSIAVTQGSFLDLSGGNGGSGT
                     NGGNGGDALMLADSVTLGGGGGNLTLKGGDAGDPGGSAGGVGLGIQNAYVGTGSTLNL
                     VTGLSGTGPAGNVVASLGDLRGSGDVVVSGTGSVLQVGSGSFSGVL"
     gene            complement(1793..>2925)
                     /locus_tag="VFR02_00335"
     CDS             complement(1793..>2925)
                     /locus_tag="VFR02_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HET9868933.1"
                     /translation="GLTLLALFLFLAAASGLWLYWSGAKGTGPGRPSFFPRPSAFQGD
                     PSQATTQLVTAVNDRLGSLGVLKLLTEPVDVEPVTAQGRIFPGYHESFRLPARFTPDA
                     LADLISQAAGQKGARLLSQAVREDGADQRTIHDYAFGFEPDWVPLRVSLVEVHRPKVC
                     LIIDDGGYQKGKALATLYQFKVPVTVSVIPDVTYSRELAGEMPSHGVEVMCHMPMQGH
                     EEAVRDDYKEFLRLGMRVGDVEEQVGRALDDLPGCVGLNNHMGSQATEDEDLMLKVCQ
                     VLKARGLYLIDSRTTARSVEADEAVKVHLVHAQRDVFLDNVETPEAIRAQLDRLVRVA
                     RRHGTAVGIGHFKLTTLETLRDAIPAIQDGGVVFVYASEVVQ"
BASE COUNT          468 a         1055 c          974 g          428 t
ORIGIN      
        1 acaggacccc gctgaaggac cccgaaccca cctgcaacac gctgcccgtg cccgagacca
       61 ccacgtcccc cgaaccccgc aggtccccca aggaagccac cacgttcccc gccggcccgg
      121 tgcccgacaa acccgtgacc aggttgaggg tggaccccgt ccccacgtag gcgttctgga
      181 tgcccaggcc cacgcccccc gcggacccgc ccgggtcgcc cgcgtcaccg cccttgaggg
      241 tcaaattgcc gccgccgccg cccagggtga ccgagtctgc cagcatcaag gcgtccccgc
      301 cgttgccgcc gttggtcccg gagcccccgt tgccccccga gaggtcgagg aaggacccct
      361 gggtgaccgc gatggaaccg atggaaacga aggcgttccc gccgttgccg ccgtcgacgc
      421 ccgagcctcc gttgcccccc gaaagggaca tgccggtggc cgaggtcagt tggacgttga
      481 tcatggtcaa ggccgcgctg ccaccgttgc cgcccgtccc cgcgctgctg ttgccgccgt
      541 tgccgccctt gaggttgaag ttggaggccg aacccgattc caggatggag acgcccgtgg
      601 aggccaggtt gccccccgcc ccgccggtca gggaggccga gccgccgttg ccggccgtga
      661 ggttgaggcc cgccccctgg gacacggtca tggaacccca gttggtcagg accgatccgc
      721 cggccccgcc cgccgtatcg cccgagccgc cctttccggc cgtcaggatc cagttggagc
      781 cggtgaccgt ggccacgccg ctggcgaccg tgaccgaccc gcccatcccg ccactatccc
      841 ccgcctggcc gttgccgccg ttcatgaaga cgcctgaact gccgtccacc gaccaattgc
      901 ccgtggacac gctcaccgcg ccgcccgcgc cgcccacgct tcccgaggcg tcggacaccg
      961 cgccgccggc cccgcccacg atggaataat tggaatcgct caagacccag gccccggtct
     1021 ggaacgaggc ctggccgccg gccccgccca cgtcattggt gccgcccagg tcggtcaccg
     1081 cgccgcccgc gccggcctgg aggctgccgc cctggatgcc gttcatggtg accgccgccg
     1141 actgccaaaa ggccttcccg ccggcctggc ccgtgtcgcc gctgccgccc gaggtgctca
     1201 ggaacccgcc gtcgccggcg tcgatgccca atcccgagtt ggcgtccatg aaaacgttgg
     1261 tcgccgtgag gcccgcgtcc tgccccgagc ccaggtcgaa aaggttcaag gccaccttgt
     1321 tcccctcgta aaaagagcct tggaagaaca gggtggattg ggacgaatcg ttcccgtaaa
     1381 ggttcacgcc gtggaaggaa ccggtgtcca cccccgtgaa actggccgcc tgggtgaagg
     1441 ccggcagggg ttggccgagt gtcacggacc cgcccccggg cgagatctgg aagaccaggt
     1501 cctggttgcc ggtggaattg gacgtgctga cggccgcccc cagggacccg gcggaggtgt
     1561 cccccgcgta ggtggtgatg gtgacgggga cctgggcgtc aagcggggcg aagggcgcgg
     1621 cggcgaggca cagaacaagg gcgcgcaaaa gggccggctt catcaaggcc tccaggttcg
     1681 ggaaatggac ggaccggaac gcaacccgcc tattgtacct tgcttcaaaa aaaaccgggc
     1741 gcccaaaggc gccccgggtc ttttcaagcg gatttatcga tttacccgcc ccttattgca
     1801 ccacttcgga agcgtacacg aacacgacgc ccccgtcttg gatcgctggg atggcgtccc
     1861 gcagggtctc gagcgtggtg agcttgaaat gcccgatgcc caccgccgtg ccgtgccgcc
     1921 gggccacgcg caccaaccgg tccagttggg cccggatggc ctccggcgtc tccacgttgt
     1981 ccaggaagac gtcgcgttgg gcgtggacca ggtgcacctt gacggcctcg tcggcctcca
     2041 cggatctcgc cgtggtgcgc gaatcgatca ggtagagccc ccgggccttg agcacctggc
     2101 agaccttcag catcaggtcc tcgtcctcgg tggcctggct gcccatgtgg ttgttgaggc
     2161 ccacgcagcc gggcaggtcg tccagcgcgc ggcccacctg ttcctccacg tcccccaccc
     2221 gcatgcccaa ccggaggaat tccttgtagt cgtcccggac ggcctcctcg tggccctgca
     2281 tgggcatgtg gcacatgacc tccacgccgt gggagggcat ttcccccgcc aattcccggg
     2341 aataggtcac gtcggggatg accgagaccg tcacgggcac cttgaactgg tacagggtcg
     2401 ccagggcctt ccccttctgg tatcccccgt cgtcgatgat caggcagacc ttgggccggt
     2461 ggacctccac cagcgagacg cgcaggggca cccaatcggg ctcgaacccg aaggcgtaat
     2521 catggatggt ccgctggtcc gccccgtcct cccggaccgc ctgggaaagc aggcgggcgc
     2581 ccttctggcc ggccgcctgg gagatcaggt cggccagggc gtcgggggtg aagcgcgcgg
     2641 gcaggcggaa gctctcgtgg taaccgggga agatgcgccc ttgggcggtc acgggctcca
     2701 cgtccacggg ttcggtgagg agcttgagca cccccaggga ccccaaccgg tcgttgacgg
     2761 ccgtgaccag ctgggtggtg gcttgggacg ggtcgccctg gaaggcggag gggcgcggga
     2821 agaaagaggg gcgccccggg ccggtaccct tggcgcccga ccaatagagc caaaggccgg
     2881 aggcggcggc caggaagagg aagagggcca gcagggtgag ccccg
//
LOCUS       DASTJJ010000031         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_739049, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000031 DASTJJ010000000
VERSION     DASTJJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739049"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFR02_00340"
     CDS             <1..1464
                     /locus_tag="VFR02_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9868934.1"
                     /translation="SGTSRKYGGTGLGLSISRELAKLLGGQLTLSSSRPGKGSTFVLY
                     LPKNYTPAKPAGMTGTRAAESLAALHSMRAPLAAAEAGLAPSVKVPDDRKNIQPGDPV
                     LLIVENDANFAHILLDAARERGYKGLVALRGDTALRLANEVRPHAVTLDIQLPDIDGW
                     KILSRFKNDLATRHIPVEIISADEDWTRGLKQGAAGFLAKPVSKEGLQRAFDDLKRFR
                     EPRVRQVLVACAKQGERARLKEALEGEDTRVQAVAGSQDALALMRSGRFDAVVVELPD
                     AEGFRVLQALHANPDWVPVPILAYLTSELTKKEEAELKRAAKAGVVQEVKTLDRLVDE
                     AALFLHQETAKLPEAKRRGIEHLHQSGAMLAGRKVLVVDDDIRNIFAMTSLLEHQQMQ
                     VLSAESGREAIEILQKTPGMDIVLMDIMMPEMDGYDTMKAIRRLEAFQSLPIVALTAK
                     AMKGDREKCIESGASDYITKPVDSEQLLALMRTWLGR"
BASE COUNT          291 a          549 c          565 g          210 t
ORIGIN      
        1 agcggcacca gccgcaaata cggcggcacg ggcctggggc tttccatcag ccgggaactg
       61 gccaagctgc tgggcggcca gctgacgctt tccagcagcc gcccgggcaa ggggagcacc
      121 ttcgtgctgt acctgcccaa gaactacacg cccgccaagc ccgcggggat gaccgggacc
      181 cgggcggccg agtccctggc cgcgctccat tccatgcgcg cgcccctggc cgccgcggag
      241 gccggcctgg ccccctcggt caaggtgccc gacgaccgca agaacatcca accgggggac
      301 ccggtgctgc tcatcgtgga gaacgacgcc aatttcgccc acatcctcct ggacgcggcc
      361 cgggagcggg gctacaaggg cctggtggcg ctgcgggggg acacggccct gcggctggcc
      421 aacgaggtga ggccccacgc ggtgaccctg gacatccagc tgcccgacat cgacggctgg
      481 aagatcctgt cgcgcttcaa gaacgacctg gccacccgcc acatcccggt ggagatcatc
      541 tcggcggacg aggactggac ccggggcctc aaacagggcg cggcggggtt cctggccaaa
      601 cccgtcagca aggaaggcct gcagcgggcc ttcgacgacc tgaagcgctt ccgcgagccc
      661 agggtgcggc aggtgctggt ggcctgcgcc aagcaagggg agcgggcgcg cctgaaggag
      721 gccctggagg gcgaggacac gcgcgtgcag gcggtggccg gcagccagga cgccctggcc
      781 ctgatgcgct ccgggcggtt cgacgccgtg gtggtcgagt tgcccgacgc cgaggggttc
      841 cgggtgctcc aggccctgca cgccaacccg gactgggtgc ccgtgcccat cctggcctac
      901 ctcaccagcg aattgaccaa gaaggaggaa gcggagctca aacgggccgc caaggcgggc
      961 gtggtccagg aggtcaagac cctggaccgc ctggtggacg aggccgccct cttcctgcac
     1021 caggaaacgg ccaagctgcc cgaggccaag cggcgcggca tcgagcacct gcaccaaagc
     1081 ggggccatgc tggcgggccg caaggtgctg gtggtggacg acgacatccg caacatcttc
     1141 gccatgacca gcctgttgga gcaccagcag atgcaggtgt tgtccgccga gagcggccgg
     1201 gaggccatcg agatcctcca aaaaacgccc gggatggaca tcgtgctcat ggacatcatg
     1261 atgcccgaga tggacggcta cgacaccatg aaggccatcc gccggctgga ggccttccag
     1321 tccctgccca tcgtggccct cacggccaag gccatgaagg gcgaccgcga gaaatgcatc
     1381 gagtccgggg cctccgacta catcaccaaa ccggtggatt cggagcagct cctggccctc
     1441 atgcggacct ggttgggccg ctaggaggtt ttccatgcct gagaccgaag cgcccgtcag
     1501 cctgctgttg gtggacgacg agcccaagaa cctgatggcg ctggaagccg tcctggaggg
     1561 gctgggccac cagctggtgc gcgccaagag cggcgaggag gccctgcgcc agctc
//
LOCUS       DASTJJ010000032         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_128056, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000032 DASTJJ010000000
VERSION     DASTJJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128056"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFR02_00345"
     CDS             <1..449
                     /locus_tag="VFR02_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868935.1"
                     /translation="PALGIWLEVVTLVIPGVNDGEGELTSIAKFLASVSRDIPWHVTA
                     FHPDYKMGRTPATGAASLNLGWEIGKAAGLRYVYSGNRPGEVGDTENTRCPACAGLLI
                     ERRGFLVLQDRLGPSGRCPDCGHPIPGVWRVPDPGKEVPAGARPRA"
     gene            493..1206
                     /locus_tag="VFR02_00350"
     CDS             493..1206
                     /locus_tag="VFR02_00350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9868936.1"
                     /translation="MFITFSDDGPASKKVPWANTFMIFVNVLVAYLTVGHKDFLQVLG
                     QYGFIPARHLGVGLFTGIFLHSSWTQLVANMSFLFFFGKGVEQEWGRVRYLAAYLLLG
                     LSGEAAHAYFHPHSTLALIGASRVVTGMGIIYLLHFPWGRMKWIFSFFGVPLLEVPSR
                     TIFVMGLWALVQAALAFIPWDILPRLMPVLARLNGTVFTVHQTAGTAWDAHLGAMAMG
                     LALFAWFTLLKKKKGRGGR"
     gene            1235..1894
                     /locus_tag="VFR02_00355"
     CDS             1235..1894
                     /locus_tag="VFR02_00355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9868937.1"
                     /translation="MLPVEEGNRLKRFPWVNVALIAVNCFVFFHLALSGGAGQREALR
                     YAFTAAHPSLLTAVTSMFLHGGILHLLGNMYFLYVFGDHLEDRLGRAAYLGAYLLSGL
                     GAVFLQYSVDPHSHIPLLGASGAISGVCALYMLMFPWQKMRWQFFFLVFPIFSVPSRA
                     IFVVGLWFLEQYLFASFGGNAEAGGVAFWAHVGGFLTGVALFPFLAAPPAKRGKAVKG
                     S"
     gene            complement(2061..3164)
                     /locus_tag="VFR02_00360"
     CDS             complement(2061..3164)
                     /locus_tag="VFR02_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9868938.1"
                     /translation="MPHATPDATKIYADRFPEFLKAGHYRDFRGLAVSSLGLGTYLGG
                     QTDAEDALYAGTIREALLSGVNLIDSAINYRCMRSERAIGSALGRLLAEGKVRREEVV
                     VCTKGGFLPFDGEPPRDPAAYFREQSQAPGLCGPGDLVAGCHCMTPAYLRDQVGRSLK
                     NLGLDAVDLYYLHNPETQLDEVPRDGFLSRLEKAFGELEKMAAEGLLRAYGTATWNGY
                     RVPPQDQDHLSLEEVLRAAERAGGSRHHFAAVQLPYNLAMTEAYGLANQLEDGKRVPL
                     LEAARRNGLAVFTSASILQSKLSRGLPAVVTENFPGLATDAQRAVQFVRSTPGVTAAL
                     VGMKRPEHLRENLGVASQAPVGEEAVARLFSGA"
     gene            complement(3263..3961)
                     /locus_tag="VFR02_00365"
     CDS             complement(3263..3961)
                     /locus_tag="VFR02_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868939.1"
                     /translation="MRAFRWGFGTFLAVVILGLGAFSQEGCGSSPSSPGGNSAGIVWY
                     SGQVLTGTYSGPGANISLRVNDSPVSDATVVLSGGFPESPVTLGYVTTETDSGKDYAV
                     YVTHSFTYVPGAGYTIASMSSALTASAGIVAPGGATFLSDASGVVTQVGEAHPGDTGN
                     LYIMDGSGNSVLSNLSGPYPIDLVDPTTYGGSSGTAYLLDANTESSTSTLTGGTMEYG
                     LTAEEDDYQPVTLP"
     gene            complement(4037..>4285)
                     /locus_tag="VFR02_00370"
     CDS             complement(4037..>4285)
                     /locus_tag="VFR02_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868940.1"
                     /translation="GNDNSVLIDGDGGTTTTLLVTNATFPVTIDPPPSGAYPGTTGDV
                     YEFDAEARTYTTSGIANAGLSFGQAAIWEEYDHSVTLP"
BASE COUNT          643 a         1445 c         1416 g          781 t
ORIGIN      
        1 tgcccgccct cgggatctgg ctggaagtgg tcaccctggt catcccgggg gtcaatgacg
       61 gcgaggggga gctgacctcc atcgccaagt tcctggcctc ggtctcccgg gacatcccct
      121 ggcacgtgac ggccttccac cccgactaca agatgggccg aacccccgcc acgggggccg
      181 cctcgctcaa cttgggctgg gagatcggca aggccgcggg cctgcgctac gtctattcgg
      241 gcaaccggcc cggcgaagtg ggcgatacgg agaacacccg ctgccccgcc tgcgcgggcc
      301 tgctcatcga gcggcggggt ttcctggtgc tccaggaccg cttggggcct tcgggccgat
      361 gcccggactg cgggcacccc atccccgggg tttggcgcgt cccggatccc ggaaaagagg
      421 tcccggcggg ggcccggccc cgggcctaat ttaccttcgg ccatccggtt tcccttttat
      481 aatgccctcc ttatgttcat caccttctcc gacgacgggc cggcctccaa gaaggtcccc
      541 tgggccaaca ccttcatgat cttcgtgaac gtgctggtgg cctacctgac cgtgggccac
      601 aaggacttcc tgcaggtgct gggccagtac ggcttcatcc ccgcccgcca cctgggggtg
      661 ggcctgttca ccgggatctt cctgcacagc agctggaccc agctggtggc caacatgtcc
      721 ttcctcttct ttttcggcaa gggcgtggaa caggaatggg gccgggtccg gtacctggcc
      781 gcctacctgc tcctgggcct ttcgggcgag gcggcccacg cctatttcca cccccacagc
      841 accctggcgc tcatcggggc ctcccgggtg gtgaccggca tggggatcat ctacctgctg
      901 catttcccct ggggccgcat gaagtggatc ttttccttct tcggggtgcc gctgctggag
      961 gtgccgtccc gcaccatctt cgtcatgggg ctctgggccc tggtgcaggc cgccctggcc
     1021 ttcatcccct gggacatcct gccccggctc atgcccgtgc tggcccggct caacggcacg
     1081 gtcttcaccg tccaccagac cgccggcacc gcctgggacg cccacctggg ggcgatggcc
     1141 atgggcctgg ccctcttcgc ctggttcacc ctgctgaaaa aaaagaaagg caggggcggg
     1201 cgctagcccg tcccgggagg gccgcttgtt cttcgtgctt cccgtggagg agggcaaccg
     1261 cctcaagcgt ttcccctggg tgaacgtcgc gctcatcgcg gtgaactgct ttgtgttctt
     1321 ccacctggcc ctctcgggcg gggcggggca gcgggaagcc ctgcgctatg ccttcacggc
     1381 cgcccacccg agcctcttga ccgccgtcac gtccatgttc ctgcacggcg ggatcctgca
     1441 cctcttgggc aacatgtact tcctctacgt gttcggggac cacctggagg accggctggg
     1501 ccgggcggcc tacctggggg cttacttatt gagcggcctg ggggcggtgt tcctgcaata
     1561 ttcggtcgat ccccattccc acatccccct gctgggcgcc tcgggggcca tctcgggcgt
     1621 ctgcgccctc tatatgctga tgttcccctg gcagaagatg cgctggcagt tctttttcct
     1681 ggtcttcccc attttcagcg tcccctcgcg ggcgatcttc gtggtggggc tttggttctt
     1741 ggagcaatac ctcttcgctt ccttcggggg gaacgcggag gccggcgggg tcgccttctg
     1801 ggcccacgtc ggcggtttcc tcaccggcgt cgcgctcttc cccttcctgg ccgccccgcc
     1861 ggcgaaaaga ggaaaagcgg tcaagggatc atgaccgaac gacaaagccg gacattttca
     1921 ccgcaaagac gcggaggcgc caagttggga tgaggatggg cggaagggcc ttccttggcg
     1981 tccccccgtc cgccccatca ttggattttt ggaacgggac cggccttggg cctgtgcggt
     2041 aagaacggtt cggtaaggcc ttaagccccg ctgaacagcc gggccacggc ctcttccccg
     2101 accggggctt gggacgccac ccccaggttc tcccgcaggt gctcggggcg cttcatgccc
     2161 accaaggccg ccgtcacgcc gggcgtggaa cggacgaatt ggacggcccg ttgggcgtcg
     2221 gtggccaggc ccgggaaatt ctcggtgacc accgccggga ggccccggga aagcttggat
     2281 tgcaggatcg aggcgctggt gaagaccgcc aggccgttgc ggcgcgcggc ctccagcagg
     2341 gggacccgct tcccgtcttc cagttggttc gccaggccgt aggcttccgt catggccagg
     2401 ttgtagggca gctgcaccgc ggcgaaatgg tggcgggacc caccggcccg ctcggccgcg
     2461 cgcagcacct cctcaagcga caggtggtcc tggtcctggg gcggcacccg gtagccgttc
     2521 caggtggccg tgccataggc gcggagcagc ccctcggccg ccattttttc caactcgccg
     2581 aaggccttct ccaggcggga caagaatccg tcgcggggca cttcatccag ttgggtctcg
     2641 ggattgtgga ggtaatagag gtcgaccgcg tccagcccca ggttcttcaa ggaccggccc
     2701 acttggtcgc gcaggtaggc gggcgtcatg caatggcagc ccgccaccag gtccccgggg
     2761 ccgcagaggc ccggggcctg ggattgttcc cggaaataag ccgccgggtc ccggggcggc
     2821 tcgccgtcga agggcaggaa tccccccttg gtgcagacca ccacctcttc ccgccggacc
     2881 ttcccctcgg ccaacagtcg tcccaaggcc gaaccgatgg cccgctcgct gcgcatgcag
     2941 cggtagttga tggccgaatc gatcaggttc acgcccgaaa gcagcgcttc ccggatggtc
     3001 ccggcgtaga gcgcgtcctc cgcgtccgtt tggccgccca ggtaggtgcc cagccccaag
     3061 gacgagaccg ccaggccgcg gaagtcgcgg taatgcccgg ccttgaggaa ctccgggaac
     3121 cggtcggcgt agattttcgt ggcgtcgggg gtggcgtggg gcacgggagt ctccttgggc
     3181 ttttccccat tctatgccat gaggccccca aaataaaaag ggcccgcccc ccaggatggg
     3241 gacgggccct gaagaaaggg ggttagggca gggtgaccgg ttggtaatcg tcctcttcgg
     3301 cggtcaaccc gtattccatg gtgccgcccg tcagggtgct tgtggaactt tccgtgttgg
     3361 cgtccaacag gtaggcggtc cccgaggatc ccccataggt cgtgggatcc accagatcga
     3421 tcgggtaggg gccgctcaaa ttgctcagga cgctgttgcc ggatccatcc atgatgtaga
     3481 ggttgcccgt gtcgcccgga tgggcctcgc ccacctgggt caccacccca ctggcgtcgg
     3541 acaagaaggt ggcgccgccc ggcgcgacga tgccggcgga ggccgtcaag gcggaggaca
     3601 tgctggcgat cgtatagccg gcgcccggca catacgtgaa gctgtgcgtc acgtaaaccg
     3661 cgtagtcctt cccgctgtcg gtctccgtgg tgacataacc caaggtcacg ggggattcgg
     3721 gaaagccgcc cgagagcacc accgtggcgt cgctgaccgg cgagtcattg acccggaggg
     3781 agatattggc tcccggcccg gagtaggtgc cggtgaggac ctgcccgctg taccaaacga
     3841 tgcccgccga gttcccgccc ggactggaag gggaactgcc gcagccctcc tgggaaaaag
     3901 cccccaagcc caggatcacg accgccagga aagtcccgaa gccccaccgg aatgcgcgca
     3961 tgccgaaccc cctcccttgg aatggggggg agtttaacct ccccgggacc ctttgccaag
     4021 ggcctcggaa ggccccttag ggcagggtga ccgagtggtc gtattcctcc cagatggccg
     4081 cctggccgaa ggaaagcccc gcgttggcga tgccgctggt cgtgtaggtc ctggcctcgg
     4141 cgtcgaactc atagacgtcg cccgtggtgc ccggataggc cccgctgggg ggcggatcga
     4201 tggtcaccgg gaaggtggcg ttggtcacca agagggtggt ggtggtcccc ccatccccgt
     4261 cgatgagcac cgagttgtcg ttgcc
//
LOCUS       DASTJJ010000033         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_862189, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000033 DASTJJ010000000
VERSION     DASTJJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862189"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFR02_00375"
     CDS             <1..433
                     /locus_tag="VFR02_00375"
                     /inference="COORDINATES: protein motif:HMM:NF037014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET9868941.1"
                     /translation="YKLSSLAGGPRLKISENVTKTSLPGVKKVVRYTDPEGFFYADGI
                     LLEDEKSVETIHHPHFPEQKSRVAHCFPESILFKVMEGGKRLVDLGVARAAEYAQERL
                     AKLSPERKRFENPHVYKVGMSRKLMDLRNELIRNSQGGPRP"
     gene            430..972
                     /locus_tag="VFR02_00380"
     CDS             430..972
                     /locus_tag="VFR02_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HET9868942.1"
                     /translation="MKALLIVDVQNDFCPGGALAVPDGDRVVPVINKILGAFPLVVAS
                     KDWHPPRTVHFDKWPPHCVQGTPGAEFHPDLDQSRIKKVFLKGSHDRDDGYSAFEATN
                     ADLAAYLREQGVEELYIAGLATDYCVKATALDAEKNGFEAFVIEDAVAAVNAKAGDDE
                     KARRAMRKAGVTLLTSDEIV"
     gene            complement(1200..1532)
                     /locus_tag="VFR02_00385"
     CDS             complement(1200..1532)
                     /locus_tag="VFR02_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868943.1"
                     /translation="MKKFLMAAALSLVFTAAPVFAATYEVVCAKCYVTKGAHGKDHAA
                     CGAKCVSGGGALALLKGDKLYVPVDGDFQSLRDQFKDKVGQMIALDGKVESKGGVNYL
                     VVDTSNKK"
     gene            complement(1622..1996)
                     /locus_tag="VFR02_00390"
     CDS             complement(1622..1996)
                     /locus_tag="VFR02_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868944.1"
                     /translation="MTGRLASHSLHYACLGTVGGKPVLTGEFAHLAQWGIGNLPGRFP
                     GLKVLRSRVEPAQVRLLLDFQRLDEDLLRVLQSYKSEVKNLLRRRGFPEAHLWQWTHE
                     EQWISTPGELAGAEALFHPPVG"
     gene            complement(1993..2874)
                     /locus_tag="VFR02_00395"
     CDS             complement(1993..2874)
                     /locus_tag="VFR02_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9868945.1"
                     /translation="MTVRIGYQPVSSAILLGKSKGFYEEAFAPLNAVVEYDLFLSGPL
                     IIEATAGKRVDLMNVGNMPPLIGRSNGVDVQVIAKAAFNPATNAVLVRPDSPIQSVED
                     LRGKRVAVQVGSSVHYFLQQVLQSRGMSLKDVHLVNLAGPDQGPALASGAVDAIVLWM
                     PYRTQLEMAGKARALVDSSNVSGNLGVYMVRGAFGTAHPDLVRAFLKATKRSNDYLRR
                     HPKEALKLLSAASQFPEKVMAVSLRGYDWSMAVGDGDIRAMDEIKKFLLQTGVLRHDF
                     KIDDLFDRSYLAATGIR"
     gene            complement(3084..>3648)
                     /locus_tag="VFR02_00400"
     CDS             complement(3084..>3648)
                     /locus_tag="VFR02_00400"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9868946.1"
                     /translation="PSGGSLELGGRKIRGPGLDRGVVFQEPRLFPWMTAGENVAFGCP
                     ARFSPREAADSVRRHLELVGLSGFEKAYPSQLSGGMQQRAAIARALVAKPRLLLLDEP
                     FGALDALTRVRMQREISRIWRAEGMTMILVTHDIDEAVFLGDRVLVLGPRPGALKRTV
                     PVKLRRPRDRNAAAFTALRRKVQSEFL"
BASE COUNT          689 a         1206 c         1158 g          595 t
ORIGIN      
        1 ctacaagctc agttccctgg cgggcgggcc ccgcctcaag atctcggaga acgtcaccaa
       61 gacctccctg ccgggcgtca agaaggtggt gcgctacacc gatccggagg gcttctttta
      121 cgccgacggc atcctgttgg aggatgaaaa aagcgtggaa accatccacc acccccattt
      181 ccccgagcaa aaaagccggg tggcccactg ttttcccgag tccatcctct ttaaagtgat
      241 ggaagggggg aagcgcttgg tggacctggg ggtggcccgg gccgccgaat acgcccagga
      301 acgcctggcc aagctttccc ccgagcgcaa acgctttgag aacccccacg tctataaggt
      361 gggcatgagc cgcaagctca tggacctgag gaacgaattg atccggaact cgcaaggggg
      421 accgcgccca tgaaggccct tttgatcgtg gacgtccaga acgatttctg tcccggcggc
      481 gcgctggccg tgcccgacgg ggaccgggtg gtgccggtca tcaacaagat cctgggcgcc
      541 ttccccctgg tggtggcctc caaggattgg cacccgcccc ggaccgtcca tttcgacaaa
      601 tggccgcccc attgcgtgca gggcacgccg ggcgccgagt tccatcccga cctggaccag
      661 agccggatca agaaggtctt cctcaagggc tcccatgacc gggacgacgg ctattcggcc
      721 ttcgaggcga ccaacgccga cctggccgcc tacctgcggg aacaaggcgt ggaagaactt
      781 tacatcgctg ggctggccac ggactactgc gtgaaggcca ccgccttgga cgccgaaaag
      841 aacggcttcg aggccttcgt gatcgaggac gccgtggccg cggtgaacgc caaggccggg
      901 gacgacgaga aggcccgccg ggccatgcgc aaggccggcg tcaccctgct cacctcggac
      961 gagatcgtct gagcgcgggc ataaaaaacg ggatcggtca cttcacaaag caaaacttgt
     1021 tttagcaatg atgaacgcag atgaacgccg atgttggatc gaaacgggcg aaagatcggg
     1081 ttttatctgc gcccatcggc gtccatcact gcatctatgg ctttgtttgc cttgccaaag
     1141 acatcgcccc ataaaaaaag ccccggacct ttcggtccgg ggcttttggc ggccggtgcc
     1201 tacttcttgt tggaggtgtc caccaccagg tagttgacgc cgcccttgct ttccaccttg
     1261 ccgtccaggg cgatcatctg gccgacctta tccttgaact ggtcccgcag ggactggaaa
     1321 tcgccgtcaa cgggcacgta aagcttgtcc cccttcagga gcgccagggc cccgccgccg
     1381 gaaacgcatt tggccccgca ggccgcgtgg tccttcccat gggcgccctt ggtcacgtaa
     1441 cacttggcgc agaccacttc ataggtcgcc gcgaaaaccg gggcggccgt gaacaccagg
     1501 cttaaggccg ccgccatcaa aaatttcttc atatcgtcct ccttcagggt ggattgactg
     1561 cttccaggtt aaaagaccgg cggagccaag ggaagccccc ccgggctttt aagggaaaag
     1621 attaaccgac cggcgggtgg aaaagcgcct cggccccggc caattccccg ggcgtggaga
     1681 tccactgttc ctcgtgggtc cattgccaca ggtgggcctc ggggaagccc cggcgccgca
     1741 gcaggttctt gacctcggat ttataggatt gcagcacccg cagcaggtcc tcgtccaagc
     1801 gctggaagtc cagcagcagc cgcacttgcg ccggctccac ccgggaccgc aacaccttca
     1861 ggcccggaaa acgccccggc aggttgccga tcccccactg ggccaaatga gcgaattcac
     1921 ccgtcaggac gggcttgccg cccacggtgc ccaggcacgc gtaatgcagc gagtgggagg
     1981 ccaagcggcc cgtcatcgga tgcccgtggc ggccaggtag ctgcggtcaa acaggtcgtc
     2041 gatcttgaaa tcatgccgca ggacgccggt ttgcaggagg aatttcttga tctcgtccat
     2101 cgcccggata tccccgtccc ccacggccat ggaccagtca tagcccctca ggctcaccgc
     2161 catgaccttc tcggggaatt ggctggcggc ggacaagagc ttcagggcct cctttgggtg
     2221 gcggcgcagg tagtcgttcg agcgcttggt ggccttgagg aatgcccgca ccaggtccgg
     2281 gtgcgccgtc ccgaaggccc cccgcaccat atagaccccc aggttcccgg acacgttgga
     2341 cgaatcgacc agggcccggg ccttgcccgc catctccagt tgggtgcggt agggcatcca
     2401 gagcacgatg gcgtccacgg ccccgctggc gagcgccggg ccctggtccg ggccggccag
     2461 gttcaccaaa tgcacgtcct tcaggctcat cccccggctt tggaggacct gctgcaggaa
     2521 atagtgcacg ctggacccca cctggacggc cacccgtttc cccctcaggt cctccacgga
     2581 ctggatgggc gagtcgggtc gcaccagcac cgcgttggtg gccggattga acgccgcctt
     2641 ggcgatcacc tggacgtcca ccccgttgga gcggccgatg aggggcggca tgttgcccac
     2701 gttcatcagg tccacccgct tgcccgcggt ggcctcgatg atgagggggc ccgacaggaa
     2761 aaggtcgtat tccaccacgg cgttcagggg cgcgaaggcc tcttcgtaaa aacccttgct
     2821 cttgcccaac aggatcgcgc tggagaccgg ttgatagccg atacggaccg tcaccggcga
     2881 agccggctgc cgtggcagtc ccggcgaagc cggctgccgt agcagtcccg gcgaagccgg
     2941 agccgaaaaa cccagggaag gggcgagggc tgagatcaaa aggcaggccg cggcaaagcg
     3001 caggggggtc atggaaatcc tccggggaaa ggcttgcccc accctaaaag gccccggcgt
     3061 tgattgcaag ggtttccggc ggctcagagg aactcggatt ggaccttccg ccgcagggcc
     3121 gtgaaggccg cggcgttgcg gtcccgggga cggcgcaatt tgaccgggac ggtccttttc
     3181 aaggcgccgg gccggggccc caacaccagc acccggtcgc ccaagaagac cgcctcgtcg
     3241 atgtcgtggg tcaccaggat catggtcatg ccctcggcgc gccaaatgcg ggaaatttcc
     3301 cgctgcatgc gcacccgggt cagggcgtcc aaggcgccga agggctcgtc cagcaagagc
     3361 agccggggtt tggccaccaa ggcccgggcg atggcggcgc gctgttgcat gccgcccgag
     3421 agctgggaag gataggcctt ctcaaagccg gaaagcccca ccagctccag gtggcggcgc
     3481 acgctgtcgg cggcctcgcg gggggaaaag cgcgccgggc aaccgaaggc cacgttctcc
     3541 ccggcggtca tccagggaaa aagccggggc tcctggaaca ccacgccccg gtccagcccc
     3601 ggcccccgga tcttccgccc gcccagttcg aggctcccgc cgctgggc
//
LOCUS       DASTJJ010000034         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1718281, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000034 DASTJJ010000000
VERSION     DASTJJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718281"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFR02_00405"
     CDS             <1..849
                     /locus_tag="VFR02_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868947.1"
                     /translation="HPHSSPLMGLTLSDTIRLADDKSDLKLFVAPRGESTDGPIAFMH
                     RVTGMVDPDAPLGHHVGQDVGHISSSVLGAALKLGGFHLEASTFNGAEPEPTQVDLPL
                     GTPNSYAFRVTQEFSPQFKAMASYAYVASPEPDAPDIRSVDRYSASAYLDLPLDDKWW
                     FHDSLIFGSIAHLDHADLLSSVCEEWLFNTDVFRIYSRLEVLQRTPNELAIGGLPDPD
                     SGRWVTAFTLGFSHSLVKWDGWDLSFGIQGTNDQLPAEYQAAYAGNPFTYQFYVQWGG
                     MQMDML"
     gene            1184..1867
                     /locus_tag="VFR02_00410"
     CDS             1184..1867
                     /locus_tag="VFR02_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HET9868948.1"
                     /translation="MKTFAKTALASLLALGLGAGAVWAGQFEGEVDYHVTMGNGDQSD
                     MTYLLKDGKARMEMTMKGHHMVDIIDLPDNKMYMLMPDQKMYMSSDIPKPDKKTTDKA
                     GAKIKKTGKTKTILGYKTDEWVAESQHGTTSVWGAKGIGYFIMGKGPGGKAPDSSWAD
                     ELRQGGFFPLEVDSKGDKHGMTMVATKVEPKSLDASLFTVPSGYKDMSDMMKNMGNMP
                     GMPSGMPKF"
BASE COUNT          381 a          596 c          597 g          343 t
ORIGIN      
        1 catccccaca gctcgcccct catggggctg accttgagcg acaccatccg cttggcggac
       61 gataaaagcg acctgaagct cttcgtggcg ccccggggcg agagcacgga cgggcccatc
      121 gccttcatgc accgcgtcac gggcatggtg gacccggatg cccccctggg gcaccacgtg
      181 ggccaggatg tgggccatat ctccagctcg gtgctggggg cggcccttaa actgggcggg
      241 ttccatctgg aggcctccac cttcaacggc gccgaacccg aacccaccca ggtggacctg
      301 ccgttgggga cgcccaattc ctacgccttc cgggtgaccc aggaattctc gccccagttc
      361 aaggccatgg cctcctacgc ttatgtggcc tcgcccgagc ccgatgcacc cgatatccgg
      421 tcggtggacc gctactcggc ttccgcctac ctggacctgc cgctggacga caagtggtgg
      481 ttccacgaca gcctgatctt cgggtccatc gcccacctgg accacgcgga cctgctctcc
      541 tcggtctgcg aggagtggct cttcaatacc gacgtcttcc gcatctacag ccgcttggag
      601 gtcctccagc gcacgcccaa tgaactggcc atcggggggc tgcccgatcc cgactcggga
      661 cgctgggtga cggccttcac cctgggattc agccactcgt tggtgaaatg ggacggctgg
      721 gacctttcgt tcggcatcca gggcaccaac gaccaattgc ccgccgagta ccaggcggcc
      781 tacgcaggga accccttcac ctaccagttc tacgttcagt ggggcggcat gcagatggac
      841 atgttgtagg ccatggggca agaaggccgg cgggcgatcc gaacggatcg gatcccaccc
      901 tggggttggg tggtcttggg ccgttgttcc accatcccct tcgccatggt cttttacaag
      961 tcgtggaaca gaccggccgg tcttggggcc ggacgccggc aaaagggttc cggatccctc
     1021 ggacggtctt ggccgacgac agggcctctt gaacggggcc cgcgaaaacc tgggttttgg
     1081 cggcgattcc ccgttttttt gcctttaaaa cccattgtgt tgggccaaaa gattcagata
     1141 aagtaacccc gctgggaaga cctgaatcaa taaaggagac gtcatgaaga ctttcgcgaa
     1201 gacggctttg gcctcgttgc tggccctcgg tctgggggcg ggcgcggttt gggcgggaca
     1261 gttcgagggg gaagtggatt accacgtgac gatgggcaac ggcgaccaga gcgacatgac
     1321 ctacctgctc aaggacggca aggcccgcat ggaaatgacc atgaagggac accatatggt
     1381 cgacatcatc gacctgccgg acaataagat gtacatgctg atgccggacc agaagatgta
     1441 catgtcttcc gacatcccca agccggacaa aaagacgacg gacaaggccg gggccaagat
     1501 caagaagacg ggcaagacca agaccatcct gggctacaag acggacgaat gggtcgcgga
     1561 gagccagcac ggaaccacct cggtctgggg cgccaagggc atcggttact tcatcatggg
     1621 caagggaccg ggcggcaagg cgccggattc ttcctgggcc gatgagctcc gccagggcgg
     1681 gttcttcccg ctggaggtcg attccaaggg cgacaagcac ggcatgacca tggtggccac
     1741 caaggtggaa cccaagagcc tggacgcctc gcttttcacc gttccctcgg gctacaagga
     1801 catgagcgac atgatgaaga acatgggcaa catgcccggc atgccctccg ggatgccgaa
     1861 gttctaaaaa aggcctttcc gggccccggc ttccggccgg ggccttttgg tgactgt
//
LOCUS       DASTJJ010000035         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1351130, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000035 DASTJJ010000000
VERSION     DASTJJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351130"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..671)
                     /locus_tag="VFR02_00415"
     CDS             complement(42..671)
                     /locus_tag="VFR02_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9868949.1"
                     /translation="METTYNQFAGKNLGRLEALSDGIFAFAMTLLALDIHVPDAGPVH
                     SERDLLRALLGLMPLVLPWILSFMTLGIFWVGQQTQLGQLKRTDRHYTWISLAFLASV
                     TAIPFSTRLLIQFAGYRLALLIYWFNILLLGLLILACWLYACRARLMKPEVTEAVSRA
                     VIQRVVRAQLLYAFGAALCLIHNNWSIGFILLVQINYALAPRFLAKWTA"
     gene            836..1120
                     /locus_tag="VFR02_00420"
     CDS             836..1120
                     /locus_tag="VFR02_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868950.1"
                     /translation="MSRTEIFLSPEAEAEFEAIQRDERPLVRDWARRYLADLVDFPPE
                     AWVDLHQRLKGVYFKSDNHVPFDIQGRVEMGPTQGVERVWVTRFRVRGGP"
BASE COUNT          260 a          353 c          427 g          215 t
ORIGIN      
        1 tttatgggga aggggcatcc cttttacaag gggagcccgg ctcaggccgt ccacttggcc
       61 aggaaacgcg gggccagcgc gtagttgatc tggaccagca ggatgaagcc gatgctccag
      121 ttgttgtgga tgaggcacag cgccgcgccg aaggcgtaga gcagctgcgc ccggaccacc
      181 cgctggatga ccgcccggga aacggcctcg gtgacctcgg gtttcatgag gcgcgcccgg
      241 caggcataga gccagcaggc caggatgagg agccccaaga gcaggatgtt gaaccaatag
      301 atcagcaggg ccaggcggta gccggcgaac tggatgagca gccgggtgga aaaggggatg
      361 gccgtgaccg aggccaggaa ggccaggctg atccaggtgt agtgccggtc cgtgcgtttc
      421 aactggccca actgggtctg ttggccgacc cagaagatgc ccagcgtcat gaagctcagg
      481 atccagggga ggaccagggg catgagcccc aggagcgccc gcagcaggtc ccgctccgaa
      541 tgcacggggc ccgcgtcggg cacgtggatg tccagcgcca acagcgtcat ggcgaaggcg
      601 aagatgccgt cgctcaaggc ctccaaccgg cccaggttct tcccggcgaa ttggttgtag
      661 gtggtttcca tggggcggag tatagcagag ccgggccctt ccaaaaaatg ggcggttttt
      721 tcgtgggccg aaattttttc aagaatgaaa atcttcatct tgtcacacat tagtatactt
      781 atgtatactt ttctcttggg cgcttgccgg cgacgcccaa gagaaagggg gccccttgtc
      841 ccggaccgag atattcctga gccccgaggc cgaggccgaa ttcgaggcca tccagcgcga
      901 cgagcgcccc ctggtgcggg attgggccag gcgctacctg gccgacctgg tggattttcc
      961 ccctgaagcc tgggtggacc tgcaccagcg cctgaagggc gtttatttca agtccgacaa
     1021 ccatgtgccc ttcgacatcc aagggcgggt ggaaatgggc cccacccaag gggtggagcg
     1081 ggtgtgggtg acgcggttcc gggtccgggg cgggccttga aaagagcata caattagcat
     1141 acaatctcct ggacgatcca ttcccgaggg aagaggaggc gttcatggaa ggcgtcatca
     1201 gctaccggcc ccgcatgggc aagcgcaagc tggagcacct ggtcaagggc aagct
//
LOCUS       DASTJJ010000036         1447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1106650, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000036 DASTJJ010000000
VERSION     DASTJJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106650"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..>1447
                     /locus_tag="VFR02_00425"
     CDS             141..>1447
                     /locus_tag="VFR02_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9868951.1"
                     /translation="MARSAAQLKFPAWTNPGWFLGQLTGRLSAPERKLVWMIGLYDFT
                     YALSSVFVNVFLFRHGNDWRVVGLFNLSQFLFVIPAFTAGGYLARRLSYLRSYQLGFV
                     FNAALYAAVLLLREDSIRHPWALGLLAGLGVGFYYLGQHALTFELVHSKRRDHFFSVS
                     LVLSSAFRILAPALSGWIIHQVNGWAQAGRWMVNGYHLVFLATLLAYLALIYESTRVR
                     VRPHQGRFRYWQTLTHPANPDWNRLMAGYFLWGVRNGVFWFATSLFIYRIFRDEWVVG
                     AYGMGSNLLAVGASALLAKWVSPGNRDFGVMASCLLMGAASALLTWRMDLAALILFAL
                     LNSVGVSWFQVAFSAASLSVIDQAFEGKKRRLEYLTIRELPLGLGRVTGMAIFLGGQQ
                     LYGEAGLRWSFLILGLAQAAVWMTVPGKANQQKKPTQRRKDAK"
BASE COUNT          217 a          500 c          420 g          310 t
ORIGIN      
        1 cgtactaact tttaatatga ccgtcagctc atttggagct acttcccgta actctgtgtt
       61 tcacccctct tttccaccgc ctcttttccg ctataatccc ccccacttca ccgggccttt
      121 ggcccgcgac aaaggggagc atggcccgtt ccgccgccca acttaaattc ccggcctgga
      181 ccaacccggg ctggttcctc ggccagttga cgggccgcct ctcggcgccc gagcgcaagc
      241 tcgtgtggat gatcggactc tacgacttca cctacgccct ttccagcgtc ttcgtgaacg
      301 tcttcctctt ccgccacggc aacgactggc gggtggtggg gctcttcaac ctttcccagt
      361 tcctcttcgt catcccggcc ttcacggcgg gcggctacct ggcccggcgg ctgagctacc
      421 taagaagcta ccaactgggc ttcgtcttca acgcggcgct ctacgcggcg gtgctgctct
      481 tgcgggaaga ttccatccgc cacccctggg ccctggggct cttggcgggg ttgggggtgg
      541 gtttctacta cctgggccag cacgccctca ccttcgagct ggtgcattcc aagcgccggg
      601 accatttctt ctcggtctcg ctggtgctct cctcggcctt ccgcatcctg gccccggcgc
      661 tttcgggttg gatcatccac caggtcaacg gctgggccca ggcgggccgc tggatggtga
      721 acggctacca cctggtcttt ttggcgaccc tcctggccta cctggccctc atctatgaga
      781 gcacccgcgt ccgggtcagg ccccaccagg ggcgcttccg ctattggcag accctcaccc
      841 atcccgccaa ccccgattgg aaccgcctga tggcgggcta tttcctctgg ggcgtgcgca
      901 acggggtctt ctggttcgcc accagcctct tcatctaccg catcttccgg gacgaatggg
      961 tggtgggggc ctacggcatg gggtcgaacc tcttggccgt gggggcctcc gccctgctgg
     1021 ccaagtgggt gtcgcccggc aaccgggact tcggcgtgat ggcctcctgc ctgctcatgg
     1081 gcgccgccag cgcgctgctc acctggcgca tggacctggc ggccctcatc ctcttcgccc
     1141 tgctcaattc cgtcggggtg agctggttcc aggtggcctt cagcgcggcg tcgctttcgg
     1201 tcatcgacca ggcctttgag ggcaaaaaac ggcggctcga atacctgacc atccgcgaac
     1261 tgcccctggg cctgggccgg gtgaccggca tggccatctt cctgggaggc caacaactct
     1321 acggggaggc gggccttagg tggtcttttt tgatcctagg cctcgcccaa gcggcagtgt
     1381 ggatgaccgt gccaggaaag gcaaaccaac aaaaaaagcc aacgcaaaga cgcaaagacg
     1441 ctaagta
//
LOCUS       DASTJJ010000037         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_739943, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000037 DASTJJ010000000
VERSION     DASTJJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739943"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1749)
                     /gene="gndA"
                     /locus_tag="VFR02_00430"
     CDS             complement(313..1749)
                     /gene="gndA"
                     /locus_tag="VFR02_00430"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET9868952.1"
                     /translation="MAAEQAKADFGIFGIAVMGANLAMNIEDHGFTVAVVNLRSDRVT
                     PFMKMNAGKKFIGAHKLEDFVNSLKTPRRIMLMVKAGDPVDDAIKILKPLLSKGDIII
                     DGGNSWFKDTQRREAELTKEGFYFFGAGVSGGEEGARFGPSIMPGGSEKAYEFVKPIF
                     EAISAKTNSGPCVTHCGPDGAGHFVKMVHNGIEYGDMQLIAEAYDILRKVLGLNAAEL
                     ADIFAEWNKGILDSFLIELTAKVLTVKDDETGKPLVDLVLDKAGQKGTGKWTAQIALD
                     MGVLIPTIVASLDGRGMSSIKDERVAASKQIAAVPITYHKAEKQALIDAVRDALYDSK
                     VCSYTQGMRLIKAASDEYKWNVNLGEMARIWKGGCIIRARLLDEIMKAYGRNASLPSL
                     LLDESFKKQIESTQANWRKAVSIAVSNGIPVPAMSASLAYYDAYRTADLPQNLTQAQR
                     DAFGSHTYERVDHLDRGFIHSEWLKNAK"
     gene            complement(1866..>2357)
                     /locus_tag="VFR02_00435"
     CDS             complement(1866..>2357)
                     /locus_tag="VFR02_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9868953.1"
                     /translation="GIPDVFVVLYVMEVLRRSALTFGWLMALQMGVSILLYLPVAKMT
                     SGLDRKPWVLLTFGFFALYPLALIHAHGLASLAAAFAVGGLREIGEPARKAMIADLAD
                     PRARGRVVGLYYLMRGLAVFPASILGGWLWTRDVHLPFEAAYGFGLAGFLYFLLRGAD
                     RRR"
BASE COUNT          425 a          733 c          770 g          429 t
ORIGIN      
        1 gaagcgcggc ccgctcccag ggccaaaccg gcccccacta cgaacagcgc cagcattttt
       61 gaaagacgca tgatctcctc ctcggacttc aagatagggc cgatctcccc ctcccctttg
      121 gacgaatggg cggggcgaag ggattccggc gttaaaaccc tgctttttta aatgaaaagg
      181 cggagcccgg ggaccccgcc ctttttcggc cgacctctat tcggcgttcc ggcaggaaga
      241 acggccgccg gaacgcgagg caatttactt tgcgttctgg cggaaagaac ggccgccgga
      301 acgcgaggca atttactttg cgttcttaag ccattcactg tggatgaagc cacggtccaa
      361 gtggtcgacc cgctcatagg tgtgggaccc gaaggcgtcc cgctgggcct gggtcaggtt
      421 ctggggcagg tccgccgtgc ggtaggcgtc atagtaggcc aggctggcgc tcatggcggg
      481 gaccgggatg ccgttggaca ccgcgatgga gacggccttg cgccagttgg cctgggtgga
      541 ctcgatctgc ttcttgaacg actcgtccaa gagcagggac ggcagcgagg cgttgcgccc
      601 gtaggccttc atgatctcgt ccaacagccg ggcgcggatg atgcagccgc ccttccagat
      661 gcgggccatc tcgcccaggt tgacgttcca cttgtactcg tcgctggccg ccttgatgag
      721 gcgcatgccc tgggtatagg aacagacctt ggaatcgtag agggcgtcgc gcacggcgtc
      781 gatgagcgcc tgcttttcgg ccttgtggta ggtgatgggg acggcggcga tctgcttgga
      841 ggccgccacg cgctcgtcct tgatgctgga catgccgcgg ccgtccaggg aggccacgat
      901 ggtggggatg agcacgccca tgtccagggc gatctgggcc gtccacttac cggtcccctt
      961 ctggcccgct ttgtccagca ccaggtcgac caggggcttg cccgtctcgt cgtccttgac
     1021 ggtcaggacc ttggcggtca gctcgatgag gaacgaatcc aggatgccct tgttccactc
     1081 ggcgaagatg tcggccagtt cggcggcgtt caggcccagc accttgcgca ggatgtcata
     1141 ggcctcggcg atgagctgca tgtcgccgta ctcgatgccg ttgtgcacca tcttgacgaa
     1201 atggcccgcg ccgtccggcc cgcagtgggt cacgcaaggg cccgagttgg tcttggccga
     1261 gatggcctcg aagatgggct tcacgaactc ataggccttc tcgctgccgc ccggcatgat
     1321 ggaggggccg aaccgcgcgc cttcctcgcc gcccgacacg cccgcgccga aaaagtagaa
     1381 gccttccttg gtcagctcgg cctcgcggcg ctgggtgtcc ttgaaccagg aattgccgcc
     1441 gtcgatgatg atgtcgccct tggacaagag gggcttgagg atcttgatgg cgtcgtcgac
     1501 ggggtcgccg gccttcacca tgagcatgat gcgccggggg gtcttgagcg agttgacgaa
     1561 gtcctccagc ttgtgggcgc cgatgaactt cttgcccgcg ttcatcttca tgaagggcgt
     1621 cacccggtcg ctgcgcaggt tcaccacggc caccgtgaag ccgtggtcct cgatgttcat
     1681 ggccaggttg gcgcccatca cggcgatccc gaagataccg aagtcggctt ttgcttgttc
     1741 cgctgccatg gatgcgtcct ccgtcgtgga aaaatggggt ccggcgattt tcaggggggc
     1801 attctaacat agaccccttt tgaaaatgga caatcctccc cgggaaaggg tttttggggg
     1861 gaaccttatc gcctgcggtc cgcgccgcgc aggaggaaat aaaggaagcc cgccaggccg
     1921 aagccatagg cggcctcaaa gggcaggtgc acgtcccgcg tccaaagcca accgcccagg
     1981 atcgacgcgg gaaagaccgc caggccgcgc atcaggtaat agaggcccac cacccggccc
     2041 cgggccctcg gatccgccag gtcggcgatc atggccttgc gggcgggttc gccgatctcc
     2101 cgcaagcccc ccacggcgaa ggcggccgcc aaggatgcga gcccatgggc gtggatcagg
     2161 gccaggggat agagggcgaa gaagccgaag gtcaagagca cccagggctt gcggtccagg
     2221 cccgacgtca ttttggccac cggcaggtac agcaagatgg acacgcccat ctgcagggcc
     2281 atcagccaac cgaacgtcaa ggcgctgcgg cgcagcacct ccatcacgta aagcaccacg
     2341 aagacgtccg ggatgcc
//
LOCUS       DASTJJ010000038         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_5691, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000038 DASTJJ010000000
VERSION     DASTJJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5691"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..978)
                     /locus_tag="VFR02_00440"
     CDS             complement(82..978)
                     /locus_tag="VFR02_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET9868954.1"
                     /translation="MNTSRSRAALRLVRPAPPEAAERAVPAAGGAAGEGRLEFARLGA
                     KTVVRRAYARAPLKWIATRRHSGAAWVSAGSLGGGLVGGDHLRLEVETGEGALGFVTT
                     QASTKVYRSGEPARQTLTARVRPGGVLAWVPDPVVCFEGADYGQVQDFHLDPGAGLIL
                     LDALTSGRHVWGERWKFRGYSSRTRILRGGGPVFYDHLELSPREGPVGERLGRFNLLA
                     FVVLVGAPLARAVETLRGETPPPLGQRPRLLQSRGRLGEDGLVWRVAAVDPEEGMGWA
                     KRKLGFLEGLLGDDPWARKSNG"
     gene            complement(959..1657)
                     /gene="urtE"
                     /locus_tag="VFR02_00445"
     CDS             complement(959..1657)
                     /gene="urtE"
                     /locus_tag="VFR02_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HET9868955.1"
                     /translation="MLLRTDHLDFAYGEAKVLHGIDLSVEARKITCVIGRNGVGKTTL
                     LKALMGVLRARSGKVWLADKDVTALPAHQRAKLGINLVPQGRDIFPGLTVEENLRIGL
                     EARTDGLKEIPEDIYGLFPVLRTMKGRRGGDLSGGQQQQLAIGRALAGNPGVLLLDEP
                     TEGIQPSIIKEIGQVLKKLAVERGITIVLVEQYLDFVREIGDSFVLMNRGRIVHQGLT
                     RELSKATVSEHLQV"
BASE COUNT          253 a          686 c          528 g          261 t
ORIGIN      
        1 cacgccggcc tggtggagca tcagcttgtc gatctcatgc ggcgtcaaat gcattggctg
       61 tccctccggt cttgaccgcg cctacccgtt cgatttgcgg gcccaggggt cgtcccccag
      121 cagcccttcc aggaaaccca gcttccgttt cgcccacccc atcccctctt ccggatccac
      181 cgccgccacc cgccacacca ggccgtcctc ccccagccgc ccccgggact ggagcaggcg
      241 gggcctctgc cccaggggcg gcggggtttc cccccggagc gtttccaccg cccgcgccag
      301 gggcgcgccc accagcacca cgaaggccag caggttgaaa cgtcccagcc gctcccccac
      361 cgggccttcc cggggggaaa gttccaggtg gtcgtaaaag accgggccgc cgccgcgcaa
      421 gatccgcgtc cggctcgaat agccccggaa cttccagcgt tccccccaga cgtgccgccc
      481 ggacgtcagg gcgtccaaga ggatgagccc cgcccccggg tccaggtgga aatcctggac
      541 ctgtccgtaa tccgcgccct cgaagcagac caccgggtcc gggacccagg ccagcacccc
      601 gcccggccgc acccgcgccg tcaaggtctg ccgcgccggc tcgccggagc ggtagacctt
      661 ggtggaggcc tgggtggtga cgaagcccag ggcgccctcc cccgtttcca cctccagcct
      721 caggtggtcg ccgcccacca atccgccccc caggctgccc gcgctgaccc aggcggcccc
      781 cgaatgccgc cgggtggcga tccatttcag cggcgcccgg gcgtaggcgc ggcgcaccac
      841 ggtcttcgcg cccagccggg cgaactccag ccgcccttcc cccgccgcgc cgcccgccgc
      901 cgggacggcc ctttcggccg cctcgggcgg ggcgggccgc accagcctca aggccgccct
      961 agacctggag gtgttcactg accgtggcct tggaaagctc gcgggtcaaa ccctggtgca
     1021 cgatgcggcc ccggttcatg agcacgaagg aatccccgat ctcccggacg aagtccaggt
     1081 actgctccac caacacgatg gtgatgcccc gctcgaccgc cagcttcttc agcacctggc
     1141 cgatctcctt gatgatggaa ggctggatgc cctcggtggg ctcgtccaaa agcagcacgc
     1201 ccgggttgcc cgccagggcc cgcccgatgg ccaactgctg ctgctgcccg cccgacaggt
     1261 ccccgccccg gcggcccttc atggtcctca acacggggaa gagcccgtag atgtcttccg
     1321 ggatctcctt gagcccgtcg gtgcgggcct ccaacccgat gcgcaggttc tcctcgaccg
     1381 tgagcccggg gaagatgtcc cggccctggg gcaccaggtt gatccccagc ttggcccgct
     1441 ggtgggcggg cagggccgtc acgtccttgt ccgccagcca gaccttgccc gaacgggccc
     1501 gtagcacgcc catgagggcc ttcaacaggg tggtcttgcc cacgccgttg cgcccgatga
     1561 cgcaggtgat cttgcgcgcc tcgaccgaca ggtcgatgcc gtggagcacc ttggcctccc
     1621 cgtaggcgaa atccagatgg tccgtcctta aaagcatgcg ctcaccttcc caggtaggct
     1681 tcgatgaccc gctcgtccga ctgcaccttc ttcaccgggc cctcgcag
//
LOCUS       DASTJJ010000039         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1351781, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000039 DASTJJ010000000
VERSION     DASTJJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351781"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFR02_00450"
     CDS             <1..656
                     /locus_tag="VFR02_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HET9868956.1"
                     /translation="PGTDSNIPGYQAVVGTGNNSQYSKEETTTNYEINRTVKHDVVAP
                     GSPKRISVGVFVDNLQPQQVDAIKSAVVAAAGLDLNRGDQIEVENMPFDNSAELASLK
                     ENQMETQQNFYLSLGKIGLLVLMVLGILLFLRSLLKPKREKQYVDSPDDLDMEEAVPM
                     PQVEDIITNDISAEDLARAEAAREAKKREAIRLEVADMANRHPENVAQIIKKWLSEE"
     gene            662..1681
                     /gene="fliG"
                     /locus_tag="VFR02_00455"
     CDS             662..1681
                     /gene="fliG"
                     /locus_tag="VFR02_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET9868957.1"
                     /translation="MAKSDKKVPLTGEQKAAALLITLGPELAAKVYQHLKEETVEQVT
                     LQIANLRKITPDEKDSVMQEVLNIAAAQEYISQGGVEYAHEILEKALGANKAMEIINR
                     LQGSLQMTPFDYIKRTDPQQLLNFIQSEHPQTIALILAHLTPDHAAIILGALPGEIQV
                     DVATRIAILDRAAPDVVMEIERVLERRISSIFTQEFTAAGGVRALAEVLNRADRSTEK
                     AIMEKLEEQNPELAEEVKRLMFVFDDLINLDNRTIQQVLREVDAKDLALARKGAKEEV
                     KEHLLKNMSSRAKAMILEDMEVMGPVRLKHAEEAQQKIVNVVRQLEEMGEIVVARGGE
                     EEVMI"
     gene            1717..2595
                     /locus_tag="VFR02_00460"
     CDS             1717..2595
                     /locus_tag="VFR02_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HET9868958.1"
                     /translation="MSKSVLTDRQYQYSGQGYVVDNVAWQDEIKRRLWEGAQTAPALE
                     AAPAVFEALAPEPVPAEPSPEEKAAQEAEALKAQAEEEARRIEETARERSVEIEKAAQ
                     ATAEKIVAAAAEKAQTEAGKIRDDAAEAGRKEGYDSGYQAGLGEGKTEGQKAYSGVVG
                     NLTSVFEKTVAERKELLGEMKPLLVELVGEALQACLGKKAKGGQVVTHFVEEALKKAV
                     GRAKLAVHLNPADVEAVEAEIQRLQTSVGAGEIELVPDARIEQGGCLLETEAGSVDVQ
                     IPTVVSQVKDSLRAGA"
     gene            2611..>3068
                     /locus_tag="VFR02_00465"
     CDS             2611..>3068
                     /locus_tag="VFR02_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HET9868959.1"
                     /translation="MNPVLNDVIPSAEEWKGRLNGLEVARENGRVEQIVGLVIESLGP
                     VASIGEACWITPNEPGVAPVLAEVVGFRQNRVLLMPLGEMRGLGPGSQVLKTGAPFKI
                     PVGEALLGRTLDAMGQPIDGKGPLMGLGEQYPVHREPPPSLERRRVEKPVA"
BASE COUNT          651 a          879 c         1084 g          454 t
ORIGIN      
        1 tgccggggac cgattccaat atccccggct atcaggccgt ggtgggcacc gggaacaatt
       61 cccagtattc caaggaagag accaccacca attacgagat caaccggacc gtgaagcacg
      121 acgtggtggc cccgggcagc cccaaacgca tctcagtggg cgtgttcgtg gacaacctgc
      181 agccgcagca ggtggatgcc atcaagtcgg ccgtggtggc cgcggccggc ctggacctca
      241 accggggaga ccagatcgaa gtcgagaaca tgccgttcga caattcggcg gaattggcca
      301 gcctgaagga gaaccagatg gagacgcaac aaaactttta cctgtcgctg ggcaagatcg
      361 gccttttggt cctgatggtg ctgggcatcc tgctgttcct caggtccctg ctcaagccca
      421 agcgcgagaa gcaatacgtc gattcgccgg acgacctgga catggaagaa gcggtcccca
      481 tgccccaggt ggaggacatc atcaccaacg acatctccgc ggaggatttg gcgcgggctg
      541 aggcggctcg ggaggccaag aagcgcgagg ccatccgtct ggaagtggcg gatatggcga
      601 accggcatcc ggagaacgtg gcgcagatca tcaagaaatg gctctcggag gagtagcttt
      661 catggcgaaa tccgacaaaa aagtaccctt gaccggcgag caaaaagcag ccgccctcct
      721 catcaccctg gggcccgaac tggccgccaa ggtgtaccag catttgaagg aggagacggt
      781 cgagcaggtc accctgcaga tcgccaacct gcgtaagatc acgcccgacg aaaaagactc
      841 ggtcatgcag gaagtgctca acatcgccgc ggcccaggaa tacatctccc agggcggcgt
      901 ggaatacgcc catgagatcc tggaaaaggc cctgggcgcc aacaaggcca tggagatcat
      961 caaccgcctg cagggatccc tgcagatgac gcctttcgac tacatcaaac ggaccgaccc
     1021 ccagcaattg ctgaacttca tccagtccga gcatccccag accatcgcgc tcatcctggc
     1081 ccacctgacg cccgaccacg cggccatcat cctgggcgcg ttgccggggg agatccaggt
     1141 ggatgtggcc acccgcatcg ccatcttgga ccgggccgcg ccggacgtgg tgatggagat
     1201 cgagcgggtg ttggaacgcc gcatctcctc catcttcacc caagaattca ccgccgcggg
     1261 cggcgtgagg gcgctggccg aggtgctgaa ccgggccgac cgctccacgg aaaaggccat
     1321 catggaaaag ctggaagagc agaatcccga gttggccgag gaagtcaaac gcctcatgtt
     1381 cgtcttcgac gacctcatca acctggacaa ccgcaccatc cagcaggtgc tgcgcgaggt
     1441 ggacgccaag gacctggcgt tggcccggaa gggggccaag gaagaggtga aggaacatct
     1501 cttgaagaac atgtccagcc gcgccaaggc catgatcctg gaggacatgg aagtgatggg
     1561 cccggtgcgc ttgaagcacg ccgaggaggc ccagcagaag atcgtcaacg tggtgcgcca
     1621 gctggaggag atgggcgaga tcgtggtggc ccgcggcggc gaagaagagg tcatgatcta
     1681 gtgatcgtcc gcgacccgtc caaggcggct tgagccatga gcaaaagcgt tctgaccgac
     1741 cggcagtacc agtattcggg ccagggttac gtggtcgaca acgtggcctg gcaggacgag
     1801 atcaagcgcc ggctgtggga aggggcccag acggccccgg ccttggaggc cgcgcccgcc
     1861 gtgttcgagg ccctggctcc cgaaccggtc ccggccgagc cgagcccgga ggagaaggcg
     1921 gcccaagagg ccgaggcgct caaggcccag gctgaggaag aggcccggcg gatcgaagag
     1981 accgcccggg aacggagcgt cgagatcgag aaggcggccc aagcgacggc ggaaaagatc
     2041 gtggccgcgg cggccgaaaa ggcccagacc gaggcgggca agatccgcga cgacgccgcc
     2101 gaggcgggcc gcaaggaagg ttacgattcg ggttatcaag ccgggttggg cgaaggcaag
     2161 accgaaggcc agaaggctta ttcgggcgtg gtgggcaacc tgacgtcggt ctttgaaaag
     2221 accgtcgcgg aacgcaagga acttttgggc gaaatgaagc ccctcttggt ggaattggtg
     2281 ggggaggccc tgcaggcctg cctgggcaaa aaggccaagg gcggccaggt ggtgacccac
     2341 ttcgtggaag aggcgctgaa gaaggccgtg ggccgcgcca agttggcggt gcatttgaac
     2401 ccggcggacg tggaagccgt ggaggccgag atccagcgcc tgcagacttc cgtcggcgcg
     2461 ggggagatcg agttggtgcc ggacgcgcgc attgagcagg gtgggtgcct tttggagacc
     2521 gaggcggggt cggtggacgt tcagatcccg acggtggtct cccaagtgaa ggacagcctg
     2581 agggccgggg cctaagggcc agggggacgc atgaatccgg tattgaacga cgtgatcccc
     2641 tcggcggagg aatggaaggg ccgtttgaac ggcctcgagg tggcccggga gaacgggcgc
     2701 gtggaacaga tcgtggggct ggtgatcgag tccctggggc cggtggcttc catcggggag
     2761 gcttgctgga tcaccccgaa tgagccgggg gtggcgccgg tgttggcgga agtggtgggg
     2821 ttccggcaga accgggtcct gctcatgcct ttgggcgaga tgcggggttt ggggccgggc
     2881 agccaggtgc tgaaaacggg ggcgcctttc aagatccccg tgggggaggc cttgttgggc
     2941 cggaccctgg acgccatggg acagccgatc gacggcaagg ggcccttgat gggcctgggt
     3001 gagcaatatc ccgtccatcg ggaaccgccg ccttcgctgg agcgacgtcg cgtggagaag
     3061 ccggtggc
//
LOCUS       DASTJJ010000040         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_6342, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000040 DASTJJ010000000
VERSION     DASTJJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6342"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VFR02_00470"
     CDS             complement(<1..1390)
                     /locus_tag="VFR02_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868960.1"
                     /translation="MNFFRLVLNRFKIRAAGVLGALALLGTAAASAGTCTVDATQTDQ
                     YIHCIGASSAWSGIGSLGQALFADDNVNGHIGITSLRARIDPNGNFAPEVNTLAQAHS
                     INPNVLLWATEWSPPAQYKANNNVNGGASNDTFLGASSGSPTRADTGLASYQVSFIQY
                     AQSHGAPLYALSIQNEPNFNPTYEACLWSAGQFAVYTQALHSALQNAGLSTKIMLPEP
                     DNTFGMNLAATAMDDPNIASMIGIIGTHLYGTPNPAPLPSYGFTHVTNQEWWETEISG
                     NSTDIPGALQEASWIQNSFVNSNMNAFHYWWLTDLISNNNLTIKAYVLGNYSKFIRPG
                     YYRMGATAVPSSGVSVSAFKNTNTSSPQTIVFVAINGNSGTTSQTFNINGVNVSSVTP
                     WVTDGSNNLVQKSPVAVSGNSFTYSLTGSSVTSFVAVVNGTSGPTATATRTATPVVSN
                     TWRVNAGGPAYTD"
BASE COUNT          267 a          404 c          540 g          309 t
ORIGIN      
        1 tgtccgtgta ggccggaccc ccggcgttca cacgccacgt gttcgacacc accggcgtcg
       61 ccgtgcgcgt cgccgtcgcc gtcgggcccg aggtcccgtt gaccaccgcc acgaagctgg
      121 tcacgctgga gccggtcagg ctgtaggtga aggaattccc cgacaccgcc accgggctct
      181 tttggaccag gttgttgctg ccgtcggtca cccagggggt cacggaggag acattgaccc
      241 cgttgatgtt gaaggtctgg ctggtcgtgc cgctgttgcc gttgatggcc acgaaaacga
      301 tcgtctgggg cgaggaagtg ttggtgttct tgaaggcgct cacggacacg cccgaggagg
      361 gcaccgcggt ggcgcccatg cggtaatagc cgggccggat gaacttgctg taattgccca
      421 gcacataggc cttgatggtg aggttgttgt tgctgatcag gtcggtgagc caccaatagt
      481 ggaaggcgtt catattggaa ttgacgaagg agttctggat ccagctggct tcctgcaggg
      541 cgcccgggat atccgtgctg ttgcccgata tctcggtttc ccaccattcc tggttcgtca
      601 cgtgggtgaa accgtaggac gggaggggcg ccggattggg ggtcccataa agatgggtgc
      661 cgatgatgcc gatcatggaa gcgatgttcg ggtcgtccat ggcggtcgcg gccaggttca
      721 tgccgaaggt attgtccggc tccgggagca tgatcttggt ggacaatccg gcgttctgga
      781 gggcgctgtg caaagcctga gtgtagacgg cgaactggcc cgcggaccaa agacaggctt
      841 cgtaagtggg gttgaaattg ggctcattct ggatggaaag ggcgtaaagg ggcgcgccat
      901 ggctttgggc atattggatg aacgagacct ggtaggacgc caatcccgtg tcggcgcggg
      961 tgggggagcc ggagctggcc cccaggaagg tatcgttgct ggccccgccg ttgacgttgt
     1021 tgttggcctt gtactgggcc gggggcgacc attcggtggc ccacaaaagg acgttcgggt
     1081 tgatcgaatg ggcctgggcc aaggtgttca cttcgggggc gaagttcccg ttggggtcga
     1141 tgcgggcccg cagggaggtg atcccgatgt ggccgttcac gttgtcgtcg gcgaagaggg
     1201 cttggcccaa gctgccgatg ccgctccagg cgctggaagc cccgatgcaa tggatgtatt
     1261 ggtccgtttg ggtcgcgtcc accgtgcagg tgccggccga agccgccgcc gtgcccagca
     1321 gggccaaggc ccccaggacc cccgccgcgc ggattttgaa acgattcaag acgaggcgga
     1381 aaaagttcat ggtcacgctc cggactttca gttccgagaa atgaaatgcg ttggcggcac
     1441 cggaacacgt cgccacaggg cgagggttcc gatgtcgatt agtgtttggg gtggaagcgt
     1501 ttattcccgc cggaaaaaat
//
LOCUS       DASTJJ010000041         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_495840, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000041 DASTJJ010000000
VERSION     DASTJJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495840"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VFR02_00475"
     CDS             <1..1429
                     /locus_tag="VFR02_00475"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9868961.1"
                     /translation="LTVKGGVGKILEYTGPGVKSLSATERATITNMGAELGATTSIFP
                     SDENTREYMKAQGRAKDWAPFAADAGAAYDEELIIDLNKLEPLIAQPHSPDNVIEVSK
                     LKNVDVQQVCVGSCTNSSYRDLMAVAKMLKGKHIHPNVSMTVTPGSKQVFEMIAKNGA
                     LADIIASGARIIESACGPCIGMGQAPASGAVSIRSFNRNFEGRSGTRDAKVYLTSPEV
                     CVACALTGKITDPRSLGKPHKIEVPDKFTWDDDGIIPPAKDPKKVEVLRGPNIKPLPI
                     KEALGEKLEGPVLLKVGDNITTDHIMPAGSKVLPLRSNIPAISEFVFNIIDPTFPKRA
                     KELGGGFVVGGDNYGQGSSREHAALAPMYLGLKAVLVKSFARIHRANLINFGILPLTF
                     LQAADYESISQGDQIEVPDLKMVMKADHPFPVKNLTTGKTIQAVHGLTERQKEIITAG
                     GLLNWTKQQTAAPAAKAKAPAKSKK"
     gene            complement(1496..>1969)
                     /locus_tag="VFR02_00480"
     CDS             complement(1496..>1969)
                     /locus_tag="VFR02_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9868962.1"
                     /translation="ALMLRPAQYGLAPARGSADASRPREAGGARDLQLTLSDQAWSAA
                     PRRAKAAPPARKAPAPGPGEVRAAPGLHNPPPPYPEEARRLGQQGTVVLEVEVDDQGR
                     ARSVRVARSSGYPLLDGSAKGTVQQWRFEPFRLGGVAVPGSLELPIIFNLQGQTP"
BASE COUNT          362 a          715 c          629 g          263 t
ORIGIN      
        1 actgacggtc aagggcggcg tgggcaagat cctggaatac acgggcccgg gcgtgaagag
       61 cctgtcggcc accgaacgcg ccaccatcac caacatgggg gccgagctcg gcgccaccac
      121 ctcgatcttc ccctcggacg agaacacccg cgagtacatg aaggcccagg gccgcgccaa
      181 ggactgggcg cccttcgccg ccgacgccgg cgccgcctac gacgaggagc tgatcatcga
      241 cctgaacaag ctggaacccc tcatcgccca gccccacagc ccggacaacg tcatcgaggt
      301 gtccaagctc aagaacgtcg acgtgcagca ggtctgcgtg ggaagctgca ccaacagctc
      361 ctaccgcgac ctgatggccg tggccaagat gctcaagggc aagcacatcc accccaacgt
      421 gtccatgacg gtcacgcccg ggtccaagca ggtcttcgag atgatcgcca agaacggcgc
      481 cctggccgac atcatcgcct cgggcgcgcg catcatcgag tcggcctgcg ggccctgcat
      541 cggcatgggc caggcgcccg cctcgggggc cgtgtccatc cgcagcttca accgcaactt
      601 cgagggccgc tcgggcaccc gcgacgccaa ggtctacctg acctcccccg aggtctgcgt
      661 ggcctgcgcc ctgaccggca agatcaccga cccccggtcg cttggcaagc cccacaagat
      721 cgaggtgccc gacaagttca cctgggacga cgacggcatc atcccgcccg ccaaggaccc
      781 gaaaaaggtc gaggtgctgc gcggccccaa catcaagccg ctccccatca aggaagccct
      841 gggcgagaag ctggaaggcc cggtgctctt gaaggtgggc gacaacatca ccaccgacca
      901 catcatgccc gcgggctcca aggtgctgcc gctgcgctcc aatatcccgg ccatctcgga
      961 attcgtcttc aacatcatcg accccacctt ccccaagcgc gccaaggagc tgggcggcgg
     1021 cttcgtggtg ggcggcgaca actacgggca aggctcgtcc cgcgagcacg cggcgctggc
     1081 gcccatgtac ctggggttga aggcggtgct ggtcaagagc ttcgcccgca tccaccgggc
     1141 caacctgatc aatttcggca tcctgccgct gaccttcctg caggccgccg actacgagtc
     1201 catttcccag ggcgaccaga tcgaggtccc cgacctcaag atggtcatga aggccgacca
     1261 tcccttcccg gtgaagaacc tcaccaccgg caagacgatc caggccgtcc acgggctcac
     1321 cgagcggcaa aaggagatca tcaccgccgg cgggctcttg aactggacca agcagcagac
     1381 cgccgccccc gccgccaagg cgaaggcgcc ggccaaatcc aaaaaataag tcgaggatgg
     1441 gtcggtcagg aagtcaaagg ccccggtccc acggaccggg gccttttttt gcgccttaag
     1501 gggtttgacc ctgcaggttg aagatgatgg gaagctccag gctgcccggg accgccacgc
     1561 cgcccagccg gaagggctcg aagcgccact gttggaccgt ccccttggcc gacccgtcca
     1621 aaagcggata gccggaactt cgggccaccc gcacggaccg cgcccggccc tggtcgtcca
     1681 cctccacctc gagcaccacg gtcccctgct ggcccaggcg ccgggcttcc tccggataag
     1741 ggggcggcgg attgtggagt ccgggcgccg cccgcacctc gcccggtccc ggcgcggggg
     1801 ccttccgggc cgggggggcg gccttcgcgc ggcggggggc ggcgctccag gcctggtcgc
     1861 tcaaggtgag ctgcaggtcg cgggcgccgc ccgcttcccg gggccgggaa gcgtcggcgg
     1921 agccgcgcgc gggggccagg ccgtattggg cgggccggag catcagcgc
//
LOCUS       DASTJJ010000042         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_617585, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000042 DASTJJ010000000
VERSION     DASTJJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617585"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VFR02_00485"
     CDS             complement(<1..1184)
                     /locus_tag="VFR02_00485"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET9868963.1"
                     /translation="MKSNVSPLQAARASYKPKLPKALAGNPSKVALKLGKPSRSISDA
                     EAIRKLFPQTYGQPEASFAAGKGSGSLKKPLKVALVLSGGQAPGGHNVVAGLFDGLKK
                     LNPKNSLTGYLGGPSGLVDNKKIEITEKLLVAYRNTGGFDIIGSGRTKLETHEQFETV
                     SRNLRAAGITALVVVGGDDSNTNAAVLAEYFKAHQIPIQVVGCPKTIDGDLKNGQIEV
                     SFGFDTATKVYAELAGNVGRDCLSAKKYWHFIKVMGRSASHIALEVALKAHPNVCLIS
                     EEVAAKDWTLAQITEQVARVVADRAAKKMNYGVVVIPEGLLEFIPEIKKLIAELNDLL
                     AHHPEFEKLETPEEKIAFVKNLLSAESKEAFKSLPHSIQAQLLADRDPHGNVQVAKID
                     TE"
BASE COUNT          218 a          411 c          442 g          284 t
ORIGIN      
        1 ttctcggtgt cgatcttggc cacctgcacg ttgccgtggg ggtcccggtc ggccaagagc
       61 tgggcctgga tcgaatgggg cagggacttg aaggcttcct tcgattcggc gctcaggagg
      121 ttcttcacga aggcgatctt ctcctcgggc gtttccagtt tctcgaactc cggatggtgg
      181 gccagcaggt cgttgagctc ggcgatgagc ttcttgatct cggggatgaa ctccaagagc
      241 ccctcgggga tgaccaccac gccgtagttc atcttcttgg ccgcccggtc ggccaccacg
      301 cgggccacct gttcggtgat ctgggcgagg gtccagtcct tcgccgccac ctcctcggag
      361 atcaggcaga cgttggggtg ggccttcagg gccacttcca gcgcgatgtg gctggccgag
      421 cggcccatca ccttgatgaa gtgccagtac ttcttggccg agaggcagtc ccggcccacg
      481 ttgccggcca gttcggcgta gaccttggtg gcggtgtcga acccgaagct gacctcgatc
      541 tggccgttct tcaggtcgcc gtcgatggtc ttggggcatc ccaccacctg gatgggaatc
      601 tggtgggcct tgaaatactc ggccagcacg gccgcgttgg tgttggaatc gtccccgccc
      661 accaccacca gggccgtgat gccggccgcg cgcaagttgc ggctcacggt ctcgaactgc
      721 tcgtgggtct ccagcttggt gcggcccgag ccgatgatgt cgaagccgcc ggtgttccgg
      781 taggcgacca ggagcttttc ggtgatctcg atcttcttgt tgtccaccag ccccgagggc
      841 ccgcccaggt agccggtgag gctgttcttc gggttgagct tcttgagccc gtcaaagagc
      901 ccggccacca cgttgtggcc acccggggcc tggccgccgg acagcaccag cgccaccttg
      961 aggggtttct tgagggaccc cgagcccttg cccgccgcga aggaagcctc cggctggccg
     1021 taggtctggg ggaagagctt gcggatggcc tcggcgtcgc tgatggatcg gctgggctta
     1081 cccagtttca aggccacctt ggaggggttc cccgccaacg ccttgggcag cttgggcttg
     1141 tacgaagcgc gcgcggcttg caggggggaa acgttggatt tcatggggcc tccggaaggt
     1201 tggctttagg cgggccatat tataggcccg gccccggagg atggcaaaat gaaagcccca
     1261 aaaaatccgc taaattcaat acttcatccc cgcttactta cgtagtatta gggaatgtca
     1321 aaaaccaatc gccctggtgg attgcaacaa cggtt
//
LOCUS       DASTJJ010000043         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_374268, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000043 DASTJJ010000000
VERSION     DASTJJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374268"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFR02_00490"
     CDS             complement(<1..222)
                     /locus_tag="VFR02_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET9868964.1"
                     /translation="MDYSIFPAINASLNGLSGVLLLAGLWCIKRGRRDLHRLCMLSAF
                     TVSVVFLACYLTYHFHAGVVHFTGQGWVRP"
     gene            complement(230..841)
                     /locus_tag="VFR02_00495"
     CDS             complement(230..841)
                     /locus_tag="VFR02_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9868965.1"
                     /translation="METKNRLTWKEWTSLGVAALALGALGFHMASLSKPAPGDWGKAP
                     AFRYEDRNGGTLRNTDLKGKFWVADFIFTRCAGSCPILSQQMRRLQERWKGNDKLALV
                     SFTVDPDYDTVAVLKGYADDLDADPKQWFFLTGAKKDLLRTAEDGFHLTAIENPGAEP
                     GALFIHSTKLVLVGPEGTILGYYDGTDKDEFNKLRAELKSLLG"
     gene            complement(850..1590)
                     /locus_tag="VFR02_00500"
     CDS             complement(850..1590)
                     /locus_tag="VFR02_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9868966.1"
                     /translation="MTYWLAVYTLLDRELVRFYRQPSRVVGALGTALLFWGVLGTGIG
                     GSAYRAYFFPGTLVMSMLFTCIFSNASVIEDRQAGFLQSVLVAPVSRGALVMGKILGG
                     AVLAFLQGFVLLALAGTVGADPTPASFLGLVLSLFLTAFVLTGMGFWLAWTLESSQGF
                     HTLMNLILLPLWMASGAVFAAGRSPWLAAFMRVNPLTYGVAAIRRGLFPGTDPSLPSL
                     GVCWAVLFALGAFLYASSAWAVEKSRIK"
     gene            complement(1587..>2176)
                     /locus_tag="VFR02_00505"
     CDS             complement(1587..>2176)
                     /locus_tag="VFR02_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9868967.1"
                     /translation="DRDLERLGLSGRAKDKVGALSGGLQRRVELAKSLLNDPELLILD
                     EPSTGLDPSARKDFWGHLEALRRDRGMTVLITTHFLEEADRCDRLLILNQGRKVALGA
                     PASLKAQLGGEVLRLKVKDPAALAPRLEAQFKVRPAEVQGLLCLEAKDAHRLVGPLME
                     AFPGDILSLTLGLPTLEDVFIHNTGRRFDDPKDAS"
BASE COUNT          393 a          745 c          718 g          320 t
ORIGIN      
        1 gggccggacc cacccttgtc ccgtgaaatg caccaccccg gcgtggaagt ggtaggtcag
       61 gtagcaggcc aggaagacca ccgagaccgt gaaggccgac agcatgcaga gccggtgcag
      121 gtcccggcgg ccgcgcttga tgcaccagag gcccgccagc agcaggaccc cgctcaggcc
      181 gttgagcgac gcgttgatgg ccggaaagat cgagtagtcc atggtcccct tatcccaaaa
      241 gcgatttcag ctcggcgcgc agcttgttga actcatcctt gtccgtgccg tcataatagc
      301 ccaggatcgt cccctcgggc cccaccagca ccagcttggt ggaatggatg aagagcgccc
      361 ccggctcggc gcccgggttc tcgatggccg tcaggtggaa gccgtcctcg gcggtccgca
      421 gcaggtcctt tttggcgccg gtcaggaaga accactgttt cgggtccgcg tccaggtcgt
      481 cggcgtatcc cttgagcacg gccacggtgt cgtaatccgg gtccaccgtg aaggagacca
      541 gggccagctt gtcgttcccc ttccaccgtt cctgcagccg gcgcatctgc tggctcagga
      601 tggggcagga gcccgcgcag cgcgtgaaga tgaagtccgc cacccagaat ttgcccttca
      661 ggtcggtgtt gcgcagcgtg ccgccgttgc ggtcctcgta tcggaaggcc ggcgccttcc
      721 cccaatcccc gggggcgggt ttggagaggg aggccatgtg gaagcccaag gcccccaggg
      781 ccagggcggc cacccccagg gaggtccatt ctttccaggt cagccggttc ttggtctcca
      841 cttgtcctcc tatttgatcc tgcttttttc caccgcccaa gccgacgagg cgtacaggaa
      901 tgcccccagc gcgaacagca cggcccagca gacccccagg gacgggagcg aggggtcggt
      961 cccggggaaa agcccccgcc ggatggccgc cacgccgtag gtcagcgggt tcacccgcat
     1021 gaaggccgcc aaccagggcg accggccggc ggcgaagacc gcgcccgagg ccatccagag
     1081 cggcagcaaa atgaggttca tcagggtatg gaatccctgg gaggattcca gcgtccaggc
     1141 cagccagaac cccatacccg tcagcacgaa agccgtcagg aacaacgaca acaccaggcc
     1201 caggaaggac gccggcgtgg ggtcggcccc cacggtgccc gccagggcca acagcacgaa
     1261 gccctgcagg aaggccagga cggcgccgcc caatattttc cccatcacca gcgccccccg
     1321 ggagaccggc gccaccagca cggactgcag gaacccggcc tgccggtcct cgatgaccga
     1381 ggcgttggaa aagatgcagg tgaagagcat gctcatgacc agggtgcccg ggaaaaaata
     1441 agcccggtag gcgctgccgc cgatgcccgt gcccagcacg ccccagaaga gcagggccgt
     1501 gcccagcgcc cccaccaccc ggctgggctg gcggtagaag cgcaccagtt cccggtccaa
     1561 cagggtatag acggccaacc agtaggtcat gatgcgtcct tggggtcgtc gaagcggcgg
     1621 cccgtgttgt ggatgaagac gtcctccagg gtgggcaggc ccagggtgag cgacaggatg
     1681 tcccccggga aggcctccat caggggcccc accaaccggt gggcgtcctt ggcctccagg
     1741 cacaagagtc cctgtacttc ggcgggacgg accttgaatt gggcctccag gcgcggcgcc
     1801 agggcggccg ggtccttgac cttgagccgc aacacctccc cgcccaattg ggccttcagg
     1861 gacgccggcg cccccaaggc caccttgcgg ccctggttca ggatgagcag tcggtcgcac
     1921 cggtccgctt cctccaggaa atgggtggtg atgagcaccg tcatgccccg gtcccggcga
     1981 agggcctcca ggtggcccca gaagtccttc cgggcggagg ggtccaggcc tgtggagggt
     2041 tcgtccagga tgagcagttc ggggtcgttc aggagcgact tggccagctc cacccggcgc
     2101 tgcaaccccc ccgagagggc gcccaccttg tccttggccc ggcccgacag ccccaggcgt
     2161 tccaggtcgc ggtcga
//
LOCUS       DASTJJ010000044         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_251271, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000044 DASTJJ010000000
VERSION     DASTJJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251271"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..2161)
                     /gene="dnaX"
                     /locus_tag="VFR02_00510"
     CDS             complement(419..2161)
                     /gene="dnaX"
                     /locus_tag="VFR02_00510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9868968.1"
                     /translation="MPPQVMALKWRPQTFADMVGQEHITRTLKNAITADRVAHAYIFA
                     GPRGVGKTTTARIFAKALNCRAPKDAEPCNACDLCEEVNQGSAMDVIEIDAASFNRVE
                     DIRNLQEQVGTYPATAKYKVYIFDEAHRITRAAFDAFHKTLEEPPAHVIFILASTEIH
                     QFPPTVQSRCQRFEFRAMNLDTLIRQLRRISDSEKIEVDDKALYQIARSAGGSMRDAQ
                     RTLDQLVAFSGRAITAEDIRVVLGTIESEVYLRFLEAAAGEDAGAALDLVRQVLEGGK
                     DLDHFYSGLLEFYRHLAVAKAYPSSAASLIPLAPEEVDRLTAVAKTCDDAYVLSALRL
                     LIEQEWAARRSSLPQVVLETLALELCRLKQLTSVAGLMGGGAASRPAAPARPAAPRPA
                     APTRSIAPAAPAASAPRPSEAPAPPADSEPVLAVEEIEDPVEAEKNPDLAKLKSQWRE
                     FLEKAAAQKKALQGVLVDTRPKSLEEGTLVLSCKGPFHQEQLSKTENKTLVEQILKEM
                     LGRPVTLVPVLAAEAPPPAEAKPSGGARAPKSMNAPAVDVKELEKEEPLVAAALKMFG
                     GKIVEVKRNAPKQL"
     gene            complement(2196..2293)
                     /gene="ffs"
                     /locus_tag="VFR02_00515"
     ncRNA           complement(2196..2293)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR02_00515"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2500..2576
                     /locus_tag="VFR02_00520"
     tRNA            2500..2576
                     /locus_tag="VFR02_00520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2534..2536,aa:Arg,seq:acg)
BASE COUNT          433 a          754 c          875 g          514 t
ORIGIN      
        1 gggcgctgcc cgtgaaggtc tgcttttcca gctcttcctg gaactgggac atcttttcct
       61 tcaaggcctg ggcctgtcgg atcatgtcga acatgttggg caagggcggg ctccggatag
      121 gattaaggaa agacggaaac cttttttacc acgaagttca ccaagttcac caagtaaaac
      181 caaggccgaa ttttgaaaat ccgatgattt ttttagaact tctaacaaaa cccatttcac
      241 caccaagaca ccaagggcac caaggactgc aaaggctgaa aagcaggaaa atccgaatga
      301 atttgatcgc ctttcttggt gaacttggtg ccttggtggt aaaaattgca tttatttttg
      361 atttttgtta gaggctctta gtgaacttcg tgcacttcgt ggtgaaaatt cttgtttttc
      421 atagttgttt tggggcgttg cgcttcactt caacgatctt gccgccgaac atcttcaggg
      481 cggcggccac cagcggttcc tctttttcca attccttcac gtccaccgcg ggggcgttca
      541 tcgacttggg cgcgcgggcc ccgcccgacg gcttggcctc ggcgggcggg ggggcctcgg
      601 cggccagcac gggcaccagg gtcacagggc gtcccaacat ctccttcagg atctgctcca
      661 ccagggtctt gttctcggtc ttggaaagct gttcctggtg gaagggtccc ttgcaggaaa
      721 gcaccagggt gccttcttcc aggctcttgg gccgggtgtc caccagcacg ccctgcaggg
      781 ccttcttctg ggcggcggcc ttttcgagga actcgcgcca ttgggatttg agtttcgcca
      841 ggtcggggtt cttttcggcc tccacggggt cctcgatctc ctccaccgcc aggacgggtt
      901 cgctgtccgc cgggggcgcg ggcgcctcgg acggacgggg agcggaggcc gccggggcgg
      961 cgggggcgat ggaccgcgtg ggcgccgccg gccggggggc cgccggacgc gccggggcgg
     1021 cgggccggga cgcggcgccc ccgcccatca agcccgccac ggaggtcaac tgcttgagcc
     1081 ggcaaagctc gagcgccagg gtctccagca ccacctgggg cagggacgag cggcgggccg
     1141 cccattcctg ttcgatcaag agccgcaggg ccgacagcac gtaggcgtcg tcgcaggtct
     1201 tagcgacggc ggtcaaacgg tcgacctcct ccggggccag ggggatcagg gaggccgccg
     1261 agctgggata agccttggcc acggccaggt gccggtagaa ttccagcagg cccgaataga
     1321 aatggtccag gtccttgccg ccctccagca cttggcgcac caggtcgagc gccgcgcccg
     1381 cgtcctcccc ggcggcggct tccaggaaac gcaggtagac ctcgctctcg atggtgccca
     1441 gcaccacccg gatgtcctcg gcggtgatgg cgcgccctga aaaggccacc aactggtcca
     1501 gggtgcgctg ggcgtcgcgc atggagccgc cggcggagcg ggcgatctgg tagagggcct
     1561 tgtcgtccac ctcgatcttt tccgagtcgc tgatgcgccg caactggcgg atcagggtgt
     1621 ccaggttcat ggcgcggaac tcgaaacgct ggcagcgcga ctgcacggtg ggcgggaact
     1681 ggtggatctc ggtggaggcc aggatgaaga tgacgtgggc gggcggttcc tccagggtct
     1741 tgtggaaggc gtcgaaggcg gcccgggtga tgcggtgcgc ttcgtcgaag atgtagacct
     1801 tgtatttggc cgtggccggg taggtgccga cctgttcctg caggttgcgg atgtcctcca
     1861 cgcggttgaa ggaggccgcg tcgatctcga tgacgtccat ggccgatccc tggttgacct
     1921 cctcgcagag gtcgcaggcg ttgcagggtt cggcgtcctt gggggcgcgg cagttgaggg
     1981 ccttggcgaa gatgcgggcc gtggtggtct tccccacgcc ccggggcccg gcgaagatat
     2041 aggcgtgggc cacccggtcg gccgtgatgg cgttcttgag ggtgcgcgtg atgtgttctt
     2101 ggccgaccat gtcggcgaag gtttgggggc gccacttcag ggccatgact tgggggggca
     2161 tcgcgacctc ggatgggggt tggcggttcc accgtggaag ccaggttgac tacaacgccc
     2221 gagcacaagc ctgaccgttg cttccttccg gacctagcgg agttggggcc ggcccgccgt
     2281 ataggacccg gcttccacgt tggaaccgct tcttcatcat aaagacaacc accggggctt
     2341 tccagacggg gccccgctat ggatggggcc cggattataa cagaagggcc gggcctttgg
     2401 cgcccggggg gacaaagaaa tccctattat acttgcgata aaaatgcgct tttgggtaat
     2461 atatcggctc ttctgcgggc ccgcccgtgg gatcgcggcg cgcccgtagc tcaactggat
     2521 agagcaactg actacggatc agtaggttgg gggttcgaat ccctccgggc gcacca
//
LOCUS       DASTJJ010000045         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_863332, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000045 DASTJJ010000000
VERSION     DASTJJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863332"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFR02_00525"
     CDS             complement(<1..1009)
                     /locus_tag="VFR02_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9868969.1"
                     /translation="MAITIKDVALKAGVSVATVSRVVNQKDIHKVGAKTQQRIEKILK
                     ELGYYPNTMARGLKTRKTYNVGVVFFWYDRPTLSDFYLTRVLDGIINFLSEKKYNILM
                     NTFSREMGDSGVYANFVNSGLVDGILAIAPPVESRFFQDLKQGALPYVLINYQVNDPQ
                     VCFVDCLNTQGIIDAVDHLMSLGHKRFGFISGDIEFSKNTLDRYNAFRYVLKKKNLPF
                     SEDFVHFGPWTEEAGMKAVEKFWKAKQPPTALLSSSDVMALGAIKQLRKNNIKVPQNV
                     AVIGFDDIPLDSTLTSVHQPIYDMGYEAAKMLWNQIEKRPLDYSHRFFPTKLVVRESC
                     GY"
     gene            complement(1154..1678)
                     /locus_tag="VFR02_00530"
     CDS             complement(1154..1678)
                     /locus_tag="VFR02_00530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9868970.1"
                     /translation="MFQRMSAAVLCVFLITGCAKGNGVSKTNAADDPKVHFHTTQGDF
                     TVELFADVPTTTKNFVDLVSSGFYDGLTFHRYVAGFVIQGGDPTGTGGGGSGKTIPLE
                     ITGHQHDAGALGMARSSDPNSASSQFYICLDAAHNLDGSYTVFGKVIQGMDNVLKLRK
                     GDKMLKVTLEPAGK"
     gene            1870..1945
                     /locus_tag="VFR02_00535"
     tRNA            1870..1945
                     /locus_tag="VFR02_00535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1904..1906,aa:Glu,seq:ttc)
     gene            1975..3030
                     /gene="argC"
                     /locus_tag="VFR02_00540"
     CDS             1975..3030
                     /gene="argC"
                     /locus_tag="VFR02_00540"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9868971.1"
                     /translation="MIKVGIYGATGYMGGEALRVLLEHPQVRLSWITSRQETELADPH
                     PNLYDSHLKFIRPEDITPCDVVFMAAPSGVAMETAPQLVQKGMKVIDLGSDFRLEDRA
                     IWEKVYKRKHTSWAHAQKAVYGLTELRRSEVRRTKLVANPGCFASAAILGLAPLVENR
                     LVDLDKITVDGLSGTAGIGAELSRPAHHAEVGNNLIPYNVVDHRHTYEMEQELSRVAG
                     KAVRVHFTPIYVPIIRGVLDVCHAFPKRKMTRAQLLELYQEYYKGEFFVKVYDLPHEK
                     GVSWQYKPYPWVNSVSGTNFNLIGMDVDEERGRVVIFSVLDSIGKGGSHAGVQNMNLM
                     FGMDETLGLTRLGLHPI"
BASE COUNT          587 a          988 c          935 g          606 t
ORIGIN      
        1 aatagccgca gctctcgcgc accaccagct tggtggggaa aaagcggtgc gaatagtcca
       61 agggcctctt ttcgatctgg ttccacagca tcttggcggc ctcgtacccc atgtcgtaga
      121 tgggctggtg gaccgaggtc agggtggaat ccagcgggat gtcgtcgaag ccgatgacgg
      181 ccacgttctg gggcaccttg atgttgttct tgcgcaactg cttgatggcg cccagcgcca
      241 tgacgtcgct ggaggacagc agggccgtgg gcggctgctt ggccttccag aatttctcca
      301 ccgccttcat gcccgcctcc tcggtccagg ggccgaaatg cacgaagtcc tctgagaagg
      361 gcaggttctt cttcttgagc acgtaccgga aggcgttgta gcggtccaag gtgttcttgg
      421 agaactcgat gtcgcccgag atgaagccga agcgcttgtg gcccagggac atcaggtggt
      481 ccaccgcgtc gatgatgccc tgggtgttca ggcagtcgac gaagcagacc tgcgggtcgt
      541 tgacctggta gttgatgagc acgtagggca gcgccccctg cttcaggtcc tggaagaacc
      601 gggattccac cggcggggcg atggccagga tgccgtccac cagcccggag ttgacgaaat
      661 tggcgtaaac gcccgagtcg cccatctcgc ggctgaaggt gttcatcagg atgttgtact
      721 tcttctcgct gaggaagttg atgatgccgt ccagcacccg ggtcaggtag aagtcgctga
      781 gggtggggcg gtcgtaccag aagaacacca cgcccacgtt gtaggtcttg cgggtcttca
      841 ggccccgagc catggtgttg gggtaatagc ccagttcctt caatatcttc tcgatgcgct
      901 gctgggtctt cgcgccgacc ttgtggatgt ccttctggtt caccacgcgg gagaccgtgg
      961 ccaccgaaac gcccgccttc aaggccacat ccttgatcgt aatcgccatg gatacctcgg
     1021 ttcttttcgc cgctcgagcc taacagaatg ccggaaagat cagggcaggc cctcgtcgcc
     1081 ggccgccgga ccggcctgaa gccgaccgcc ccatgaccat tcccggatta tacaacgcca
     1141 cccgccggac gggtcacttc cccgccggtt ccagggtcac cttgagcatc ttgtcgcctt
     1201 tgcgcagttt caggacattg tccatcccct ggatcacctt gccgaagacc gtgtaggaac
     1261 cgtccaggtt gtgggccgcg tccaggcaga tatagaactg gctggaggcc gaattggggt
     1321 ccgagctgcg ggccatgccg agcgcgcccg cgtcgtgctg gtggccggtg atctccagcg
     1381 ggatggtctt gcccgagccg cccccgcccg tccccgtggg gtcgcccccc tggatgacga
     1441 accccgccac gtagcggtgg aaggtcaggc cgtcgtagaa gcccgaggac accaggtcga
     1501 cgaaattctt cgtggtggtg ggcacgtcgg cgaagagctc caccgtgaag tcgccctggg
     1561 tggtgtggaa atgcaccttg ggatcgtccg ccgcgttggt cttggacacg ccgtttcctt
     1621 tcgcgcaacc cgtgatgaga aaaacacaaa gcaccgccgc cgacatccgc tggaacatag
     1681 ggtctccttg gttgaagagg caggttttca tcatagcggc tagcccgggc ggccgtcaaa
     1741 atgaaagcgc ccccgggcgt ccttcctttc cttgacttcc ccccacccct cctctagaat
     1801 ctgctctctt ttaaccggct ttcgggcctt ccgggccctc cggctgaagg ttttcttttt
     1861 aaaacgcccg accccatcgt ctagaggcct aggacacctg gttttcatcc aggcaacacg
     1921 ggttcgaatc ccgttggggt cgccattttc actcccgtcc ccgtggagac ccacatgatc
     1981 aaagtcggca tttatggagc caccggttac atgggcggcg aggccctgcg cgtcctgctg
     2041 gaacatcccc aggtccgcct gtcctggatc accagccgcc aggagaccga gctggccgac
     2101 ccccacccca acctttacga ttcccacctc aagttcatcc gtcccgagga catcaccccc
     2161 tgcgacgtgg tcttcatggc cgcgccctcg ggcgtggcca tggaaacggc gccccaattg
     2221 gtccaaaagg ggatgaaggt catcgacctg gggtccgact tccggctcga ggaccgggcc
     2281 atctgggaga aggtctacaa acgcaaacac accagctggg cccacgccca aaaggccgtc
     2341 tacggcctca ccgagctgcg ccgttccgag gtccggcgga ccaaattggt ggccaacccc
     2401 ggttgtttcg cctcggcggc catcctgggg ctggccccct tggtggagaa ccgcctggtg
     2461 gacctggaca agatcacggt ggacggcctg tcgggcacgg cgggcatcgg cgccgagctg
     2521 tcccggccgg cccaccacgc cgaggtgggc aacaacctga tcccctacaa cgtggtcgac
     2581 caccggcaca cctacgagat ggagcaggag ctttcccggg tggcgggaaa agcggtgcgc
     2641 gtccacttca cccccatcta cgtgcccatc atccggggcg tgctggacgt ctgccacgcc
     2701 ttccccaagc ggaagatgac ccgcgcccag ttgctggagc tctatcagga atattacaag
     2761 ggcgagttct tcgtgaaggt ctatgacctg ccccacgaga agggcgtgtc ttggcagtac
     2821 aagccctacc cctgggtcaa cagcgtatcg ggcaccaact tcaacctcat cggcatggac
     2881 gtggacgagg aacggggccg ggtggtcatc ttcagcgtgt tggacagcat cggcaagggc
     2941 ggctcccacg ccggcgtcca gaacatgaac ctcatgttcg gcatggacga gaccctgggg
     3001 ttgacccgcc tgggcctgca cccgatctga aaaaccagtt cttggtttta agagtctcta
     3061 acaaaaatca aaataaaggc aattttcacc accaaggcac caagttcacc aagaaa
//
LOCUS       DASTJJ010000046         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1352500, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000046 DASTJJ010000000
VERSION     DASTJJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352500"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFR02_00545"
     CDS             complement(<1..771)
                     /locus_tag="VFR02_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET9868972.1"
                     /translation="MRHLALKSLFILLGAAAPLFGQETLHLTIGDSKLLRLQATDKIA
                     VGNPSIADVTTVSNDEILLSAKAVGTTSLTFIGADGRQQSQTVVVTAHNLKKAMIEVG
                     VEIMEIQSQSALEAGLSWGSVAAAPATGSAGAAVPNSLVLNEASPPPLLSFGSLNRQA
                     ITASLQLLIDRGKAKILAKPKLLTVSGEEASFLAGGEVPYVVQNSLGSSSVQFKPYGV
                     KLNIKPTLDADGNIAASLRAEVSGIDAQNGVALNNAVIP"
     gene            complement(782..1534)
                     /gene="cpaB"
                     /locus_tag="VFR02_00550"
     CDS             complement(782..1534)
                     /gene="cpaB"
                     /locus_tag="VFR02_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9868973.1"
                     /translation="MKDKKTLAALAAGSTAGLALFWFLSQRIDDYEKRSTPTEVLVAT
                     AFIPAQSVLRPDQVEKRKIPGAFIAPSAIQDLKEVAGLVTLAPLSAGEQVLSNKFGLP
                     GNSLVLTLAPGRRALTLEVNEVTGVAGLLSPGDRVDLIGRLDTVSRQVTATVLQDIQV
                     LAVGSTTGTGGGPAAPNAYDTVTLSLTPEQAELVSFLQERHALRLVLRPAGDDDLVPL
                     PPQSDAELAAKIGTFTPSKAKGLEVIRGIETR"
     gene            complement(1557..>1857)
                     /locus_tag="VFR02_00555"
     CDS             complement(1557..>1857)
                     /locus_tag="VFR02_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868974.1"
                     /translation="RPSGGGGPWDLPFVPPPDATASYLERFQRASLGVILAPPRGADW
                     FEAACRAQGFTERPLDQGQASRSYLIQKGPRRLIARFTTDAQRSSLALLELEAGP"
BASE COUNT          276 a          578 c          691 g          312 t
ORIGIN      
        1 cgggatgacg gcgttgttca gggccacccc gttctgggcg tcgatgccgc tcacctcggc
       61 ccggagggac gcggcgatgt tcccgtccgc gtccagcgtg ggtttgatgt tgagtttgac
      121 cccgtagggc ttgaactgca cgctggagga gcccaggctg ttctggacga cgtagggcac
      181 ctcgccgccc gccaggaaac tggcctcctc cccgctgacc gtcaacaact tgggcttggc
      241 caggatcttg gccttgccgc ggtcgatcaa aagttgcagc gaggccgtga tggcctgtcg
      301 gttcagggaa ccgaaggaca gcaggggcgg cggcgaggcc tcgttcaaca ccaggctgtt
      361 ggggaccgcc gccccggcgg agccggtggc gggggcggcc gccaccgacc cccagctcaa
      421 gccggcctcc agggcgcttt gggattggat ctccatgatc tcgacgccca cctcgatcat
      481 ggccttcttc aggttgtggg cggtgacgac gacggtctgg ctttgctgcc gcccgtcggc
      541 gccgatgaag gtgagggagg tggtgccgac cgccttggcg gagaggagga tctcgtcgtt
      601 gctgacggtg gtcacgtcgg cgatggaggg gttgcccacg gcgatcttgt cggtggcctg
      661 gaggcgcagc agcttggaat ccccgatggt caggtgcagg gtctcctggc cgaagagcgg
      721 cgcggccgcc cccagcagga tgaagaggct cttcaaggcg agatggcgca tggtccgctc
      781 cttatcgggt ctcgatgcca cggatgacct ccaggccctt ggccttggaa ggggtgaagg
      841 tgccgatctt ggccgccagt tcggcgtcgc tttggggggg caggggcacc aggtcgtcgt
      901 cgcccgccgg ccgcaggacc agccgaaggg cgtggcgttc ctgcaggaag gagaccagtt
      961 cggcctgttc gggggtgagg gacagggtga ccgtgtcata ggcgttcggg gcggcgggcc
     1021 cgccgccggt cccggtggtg gaaccgacgg ccaggacctg gatgtcctgg agcacggtgg
     1081 cggtgacctg gcgggaaacc gtgtccaggc gcccgatcag gtccacccgg tccccggggg
     1141 acagcaggcc cgcgaccccg gtcacttcat tcacctccag ggtcagggcc cggcgccccg
     1201 gggcgagggt caggaccagg ctgttgcccg gcaggccgaa cttattggac aggacctgct
     1261 caccggccga gaggggggcc agggtgacca atcccgcgac ctccttcagg tcttggatgg
     1321 cgctgggggc gatgaaggcg cccgggatct tccgtttctc gacctggtcg ggccggagga
     1381 ccgattgggc cgggatgaag gcggtggcca ccaagacctc ggtgggggtc gacctctttt
     1441 cgtagtcatc gatgcgctgg ctcaagaacc agaacagcgc caggcccgcc gttgaaccgg
     1501 ccgccagggc ggccaatgtt tttttatcct tcatcctttt tcctttcttg ggacgctcaa
     1561 ggtccggcct caagctccag cagcgcgagc gacgatcttt gggcgtccgt cgtgaaccgg
     1621 gcgatcagcc ggcgcgggcc tttctggatc aggtaggacc gggaagcttg cccctggtcc
     1681 agcgggcgtt cggtgaagcc ctgcgcgcgg caggccgcct cgaaccaatc cgccccccgg
     1741 ggcggggcga ggatgacccc cagggaggcg cgttggaagc gctccagata agaggcggtg
     1801 gcgtccgggg gcgggacaaa gggaaggtcc caagggcccc cgcccccgga cgggcgg
//
LOCUS       DASTJJ010000047         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_741272, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000047 DASTJJ010000000
VERSION     DASTJJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741272"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFR02_00560"
     CDS             <1..339
                     /locus_tag="VFR02_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9868975.1"
                     /translation="WPGNVRELENTIERMAVMAKSDNLALEDIPLPLNAYNTQLAWKV
                     EEAEEPAPAAGTRNDGTLADLEKKQVMEAMERCGGVQAKACKLLGITPRQLGYRLKKY
                     KIGYKPTFLG"
     gene            357..1091
                     /locus_tag="VFR02_00565"
     CDS             357..1091
                     /locus_tag="VFR02_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868976.1"
                     /translation="MPEPAVHLVVATPCYGGQLTTAYFTSCLKLQRACRERGLGLTFL
                     MAGEDALITRARQDLTARFMAQPSATHLLFIDADIGFEPGQVFRLLDFGADVTAAAYP
                     LKRVHWQRVGELAAQRPERLEPASLSYVFQLLTPPEVRDDFARVNYAGTGFLMARRAA
                     LEALARRHPQLRYGGEGFHYAFFDCLIEGGAFLPEDYSFCKRWTDGGGEIWLDLRSRL
                     DHVGPVTFHGDLSAHPALSETPRGDA"
     gene            1088..1765
                     /locus_tag="VFR02_00570"
     CDS             1088..1765
                     /locus_tag="VFR02_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868977.1"
                     /translation="MSPSFSLALGTAAFGGQVTLPYAASVLAVEEACAQRGIPFQYIL
                     QSGDALVQRARQQVAAQFLGTPGATHLLMVDSDIVFSPEQAFRLLESGKDLAAAVYPR
                     KIVGEPSYEYEPWEPSRIEGGFRRAKWVGAGFLLLKREALLKMEEAHPELRYSAGFIA
                     GDPLAQNPHRSALFNSMVDPKTGEYLADDRAFCRRWTDLGGEIWVDTQSRLRHIGPRT
                     WEGDLGR"
BASE COUNT          374 a          673 c          684 g          398 t
ORIGIN      
        1 tggccgggga acgtgcggga gctggagaac accatcgagc gcatggcggt catggccaag
       61 tcggacaacc tggccctgga ggacatcccc ctgcccctga acgcctacaa cacccaattg
      121 gcctggaagg tggaggaggc ggaggagccg gcgcccgcgg ccgggacgcg caacgacggc
      181 acgctggcgg acctggaaaa gaagcaggtc atggaggcca tggagcgttg cggcggcgtg
      241 caggccaagg cctgtaaact attgggcatc acgccccggc aattggggta caggctcaaa
      301 aaatataaaa taggatataa acccaccttc ctgggttgac cgtcctggag acgcctttgc
      361 ccgagcccgc cgtccatctg gtggtggcca ccccctgcta cgggggccag ctcaccaccg
      421 cctatttcac ctcctgcctc aagctccagc gcgcctgccg ggaaaggggc ctgggcctca
      481 ccttcctcat ggccggggag gacgcgctca tcacccgggc ccgacaggac ctgacggccc
      541 ggttcatggc gcagccctcg gccacccacc tgcttttcat cgacgccgac atcgggttcg
      601 agcccgggca ggtcttccgg ctgctggatt tcggcgccga cgtcacggcg gcggcctatc
      661 ccttgaagcg ggtccactgg caaagggtgg gggaactggc ggcgcagcgg ccggagcggt
      721 tggaacccgc ctcgctttcc tacgtgttcc aactgctgac gccgcccgag gtgcgggacg
      781 atttcgcccg ggtgaattac gcgggcaccg ggttcctgat ggcgcgccgc gccgcgctgg
      841 aggccctggc ccgccggcat ccccaactgc gctacggcgg cgaggggttc cactacgctt
      901 tcttcgattg cctcatcgag gggggcgcct tcctgcccga ggactatagt ttctgcaagc
      961 gctggaccga cgggggcggg gagatctggc tggatcttcg cagccggctt gaccacgtgg
     1021 gcccggtgac cttccacggc gacctatcgg cccatccggc cctgagcgag acgccgaggg
     1081 gggacgcgtg agcccttcct tttccctggc cctggggacc gccgccttcg gcggacaggt
     1141 cacgctgccc tacgcggcct ccgtgctggc ggtcgaggaa gcctgcgccc aacggggaat
     1201 ccctttccaa tacatcctgc aatcgggcga cgcgctcgtg caacgggcgc gccaacaggt
     1261 ggcggcccag ttcctaggga ctcccggcgc cacccatctt ttgatggtgg actcggacat
     1321 cgttttttcg cccgaacagg ccttccgcct gttggagtcg ggaaaggatc tcgcggcggc
     1381 ggtctatccc cggaagatcg taggggaacc ttcttatgaa tatgagccct gggagccttc
     1441 ccggattgaa gggggattcc gccgggccaa gtgggtgggc gcggggttcc ttctcttgaa
     1501 gcgggaggcc ctccttaaga tggaggaagc ccatcccgaa ctgcgttact cggcgggttt
     1561 catcgcgggc gatcccctgg cgcagaaccc gcaccgttcg gcccttttca acagcatggt
     1621 ggatcccaag accggcgaat acctggcgga tgaccgggcc ttctgccgcc gctggaccga
     1681 cctgggcggg gagatctggg tggacaccca aagccgcctg cggcatatcg gtccccgaac
     1741 ttgggaaggg gatctggggc ggtaagggcc gtgcccggcc gccgggaaaa ggcctatttg
     1801 caggccattg gggcagtttt aaccttttac caaaattctt aaaaagtgct atatttaggg
     1861 ttgcgcctgt taagggcgtt gaccgaaaag ttcggaattt gaccggttta gcatgggccg
     1921 gatcacggtt caatgcgtca aaaagaatag gcctttttgc gggccctcat aaaaacgttt
     1981 ccaatttccc aggggtgaat ggccgtccca tgaacctgcc tctcatacga aaaaccattt
     2041 ttgtcccgtt ggtccttgtc ggcttgttat ccgtgctgcc cgggacaaaa cccgccttcg
     2101 cggcggtggt caccttcaac aacatttcc
//
LOCUS       DASTJJ010000048         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1230225, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000048 DASTJJ010000000
VERSION     DASTJJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230225"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFR02_00575"
     CDS             complement(<1..575)
                     /locus_tag="VFR02_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9868978.1"
                     /translation="MKKTAVKTLKAAPAVIRLAGVSRHYGEGEARVKALDHVTLEIKK
                     GEFTAVVGPSGSGKTTLLNLIGGLDRPSEGRLWLGDREMGRMDGNELSDFRRDHLGFI
                     FQAYNLVPVLTVRENVEYVLLLQGVEEGERDRRVSRMLREVGLSGKEDRFPNELSGGQ
                     QQRVAVARAMVSEPDLILADEPTANLDSASGA"
     gene            complement(638..>1342)
                     /locus_tag="VFR02_00580"
     CDS             complement(638..>1342)
                     /locus_tag="VFR02_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9868979.1"
                     /translation="GELEQEMFRVRGIFSFNAAGMDRDLAFIGIARAQKLAGLGGQVH
                     EVALRFQRGDGSEASHRPLYEKLSQGGNEALDWSRLMPDMKSAMDLSGFSSAILAVLL
                     AGLAGLAVLNTLFMALSERLFEFGVLRALGTRPARLALLVLCESAVMGFFSVLLGTLL
                     GVGATLLLSYTGINYSGIEFAGVTFQKAIHPQWRVLQVTAFPLGVWAFTVLIALYPAL
                     HAARIVPAKALHRSLG"
BASE COUNT          252 a          481 c          402 g          207 t
ORIGIN      
        1 gcgccggagg ccgaatccag gttggcggtg ggttcgtcgg ccaggatcag gtccggttcg
       61 gacaccatgg cgcgggccac ggccacccgt tgttgctgcc cccccgaaag ctcgttgggg
      121 aaacggtcct ccttgcccga aaggcccact tcccggagca tgcgggagac ccgccggtcc
      181 cgttcgccct cctccacgcc ctgtaagagc agcacatact cgacgttctc ccgcacggtc
      241 aggaccggca ccaggttgta ggcctggaag atgaatccca ggtggtcccg gcggaagtcg
      301 gaaagctcgt tcccgtccat gcgccccatt tcccggtccc ccagccagag gcgcccctcg
      361 gagggccggt ccaacccccc gatcaggttc aaaagcgtgg tcttgcccga gcccgagggc
      421 cccaccaccg ccgtgaactc cccttttttt atctccaagg tcacgtggtc caatgccttc
      481 acgcgggctt ccccttcgcc gtaatggcgg gaaacgcccg ccagccggat cacggccggc
      541 gcggccttga gggtcttcac ggcggtcttc ttcatgaaag cctcctgaac attcctgcca
      601 tccgcccgcc taaacggcgg gaggatcctt cttggcttca gcccaaactg cggtgcaggg
      661 ccttggccgg gacgatccgg gccgcgtgca gcgccggata gagggcgatc aacaccgtga
      721 aggcccatac ccccagcggg aaggccgtga cctgcaggac cctccactgg ggatggatgg
      781 ccttctggaa ggtcacaccc gcgaattcga tgcccgaata gttgatgccc gtataggaaa
      841 gcagcagggt ggcccccacc cccaaaaggg tccccaacaa cacactgaag aagcccatga
      901 cggccgactc gcagagcacc aaaagggcca gcctggccgg gcgggtcccc agggcccgca
      961 gcaccccgaa ctcgaaaagc ctctccgaaa gcgccataaa gagcgtgttc aggacggcca
     1021 ggcccgccag gcccgccagc aggacggcca ggatggcgga ggaaaaaccc gaaaggtcca
     1081 tggccgactt catgtcgggc atgaggcggc tccagtccag ggcctcgttc cccccctggg
     1141 aaagtttttc gtaaaggggc cgatgggacg cctccgaccc gtcgccccgc tggaaccgca
     1201 gggccacctc gtggacttgc ccgcccaatc ccgccagctt ttgggcccgg gcgatcccga
     1261 tgaaggccag gtcccggtcc atgcccgcgg cgttgaatga aaagatcccc cgcacccgga
     1321 acatttcctg ctccaactcc cc
//
LOCUS       DASTJJ010000049         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_741615, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000049 DASTJJ010000000
VERSION     DASTJJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741615"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFR02_00585"
     CDS             <1..684
                     /locus_tag="VFR02_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9868980.1"
                     /translation="SIPLSFAQNTIFNVVLSFVCLGGLFFLETHHEFNGAPLQAMVWS
                     FMAGLVGVVAVMVLIFFNKAFRRRFLRVLLQAGTWLSRRVFKKSLNPIRLWATRRKIE
                     GSVKYLHQGWGRLGQVFFWVSMDWCFTALTLFFCFQAAGVQLSFGLLLVGFAVMFLTS
                     TANVVPAGLGVSEGSLAGVFAALGMDINHTLVAGLLFRVIFFFIPLAVSTGLYLDTMR
                     TLWKNQAKA"
     gene            870..>1623
                     /locus_tag="VFR02_00590"
     CDS             870..>1623
                     /locus_tag="VFR02_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9868981.1"
                     /translation="MEKLPFFSEEKSLGTYLKEIARVPLLTPEEEKRLSEAARGGDEK
                     ALTHLIEANLRFVVSVAKGYRNRGLSFLDLINEGNLGLMKAARTFDPARGVRFVSYAV
                     WWIRQSILAAILDKADIVRIPQSQSKKMRKIGKRVAELRQKAGQELSDVEVMKKAEID
                     RRTLSEVQQFTHGYLSLDTTKVGDSETPILDFLADPRDADAFEKQLMDRDLTAKLGGL
                     LKELDPRDAKILSWRYGLDGQGIRTLDEIGKSL"
BASE COUNT          305 a          483 c          498 g          337 t
ORIGIN      
        1 agcatccccc tgtccttcgc ccagaacacg atcttcaacg tggtgctttc cttcgtgtgc
       61 ctgggcgggc tttttttcct ggagacccac cacgagttca acggcgcgcc gctccaggcc
      121 atggtctgga gtttcatggc cgggttggtg ggggtggtgg ccgtcatggt gctgatcttc
      181 ttcaacaagg ccttccgccg gcgtttcctg cgggtgctcc tccaggcggg gacttggttg
      241 agccggcggg tcttcaagaa aagcctgaac ccgatccgcc tctgggccac ccgccgcaag
      301 atcgaggggt cggtcaagta cctgcaccag ggctggggaa ggctgggcca ggtctttttt
      361 tgggtgagca tggactggtg tttcacggcc ctgacccttt tcttctgctt ccaggccgcc
      421 ggggtccaat tgtccttcgg gcttttgctg gtgggcttcg cggtgatgtt cctgacctcg
      481 accgccaacg tggtgccggc ggggttgggc gtgtccgagg ggtccctggc aggggtcttt
      541 gccgccctgg ggatggacat caaccacacc ctggtggcgg ggctgctctt ccgggtgatc
      601 ttctttttca tccccctggc cgtctccacc ggcctttacc tggacaccat gcggaccctc
      661 tggaagaacc aagccaaagc ctaacccctc aagagaagcc ccccctttag ccttttcaag
      721 ccagtgattt ctcccataaa aaacggccat tttggctgaa ttttgctaag tgttttttat
      781 attagttgac atgaagtgac tcatattata tatttagacc cgtaaggaaa tatgaataag
      841 agataaggat ctcttgaagg agatgcgcca tggaaaaact tccctttttc agcgaggaaa
      901 aatcgctggg aacctatctg aaagaaatcg cccgggtgcc gcttttgacg cccgaggaag
      961 aaaagcgcct cagcgaggcc gcccggggcg gcgatgaaaa ggccctgacc cacctgatcg
     1021 aggccaacct gcgtttcgtg gtgtcggtgg ccaagggcta ccgcaaccgc gggctgtcgt
     1081 tcctggacct catcaacgag ggcaacctgg gattgatgaa ggccgcccgg accttcgacc
     1141 cggcccgggg cgtgcgcttc gtgtcctacg ccgtgtggtg gatccgccag tccatcctgg
     1201 ccgccatcct ggacaaggcc gacatcgtgc gcatcccgca gagccagtcc aagaaaatgc
     1261 gcaagattgg caagcgcgtg gccgagctgc ggcagaaggc cggccaggag ctcagcgacg
     1321 tggaggtcat gaaaaaggcc gagatcgacc gccgcaccct gagtgaggtg cagcaattca
     1381 cccacgggta cctgtcgctg gacaccacca aggtggggga cagcgagacg cccatcctgg
     1441 atttcctggc cgacccccgg gacgcggacg cctttgagaa gcagttgatg gaccgcgacc
     1501 tcacggccaa gttgggcggg cttttgaagg aactggaccc ccgggacgcc aagatcctgt
     1561 cctggcgtta cggcctggac ggccagggca tccgcaccct ggacgagatc gggaagagcc
     1621 tgc
//
LOCUS       DASTJJ010000050         1296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_864473, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000050 DASTJJ010000000
VERSION     DASTJJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864473"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..>1296)
                     /locus_tag="VFR02_00595"
     CDS             complement(65..>1296)
                     /locus_tag="VFR02_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9868982.1"
                     /translation="GLQREDPSLPGEPPLEQFRARMVRLFTNGILFFFLLGALRNLAL
                     IPARWPVILGCVSVLSLFRLGLGMRWFAPRALPWAFAASLFLSFLPSFYYWGRGSLTP
                     PLFVIPVFVIAVSLTGPILLGLAALALYLAVVGWYWLSPAAWTQGQFYVLAYLALLAP
                     DLLAAGWIFWRLRLRRLESLAAQVEGHRRSLRMRRRLMGTLLHDMRNPLVVLASLAAD
                     KGPMDAEAARDMTDRMADILQAGRALLDVEGLVPRHRLRPVAWGDLLADTESLFASRM
                     RDKELRLEKSGDPALRALALPDLLVNSVLANLLSNALKFSPRGSAITLEASERDGRLA
                     IRVLDRGPGLTPPQLESFRKGQRLANSPGTEGEPGTGLGLFLSRDYARFMGGDLQLLA
                     RDGGGTEALLTLESAEA"
BASE COUNT          227 a          437 c          493 g          139 t
ORIGIN      
        1 aggccgacta ccgccgggcc ctgcggtggc agccccacaa cgccacggcc ctgcggcacc
       61 tgcgttaggc ttcggccgat tccagcgtga gcagggcttc cgtgccgccg ccgtcccgcg
      121 cgagcagttg gaggtccccc cccatgaagc gcgcgtagtc ccgcgacaga aaaagcccca
      181 ggcccgtccc gggctcgccc tcggtgccgg gggagttggc cagcctttgt cccttccgga
      241 acgattccaa ttgcgggggc gtgagccccg gcccccggtc caggacccgg atggcgaggc
      301 gcccgtcccg ctcggaggcc tccagggtga tggccgaccc ccggggcgag aatttcaacg
      361 cgttggaaag caggttggcc agcaccgagt tcaccaacag gtcgggcagc gccagggccc
      421 gcagcgcggg gtcgcccgac ttttccaggc gcagttcctt gtcgcgcatc cgggaggcga
      481 agagcgattc ggtgtccgcc agaaggtccc cccaggccac cggcctgagg cggtggcgcg
      541 gcaccaggcc ctccacgtcc aaaagggcgc ggccggcctg caggatgtcg gccatgcggt
      601 ccgtcatgtc ccgggcggcc tcggcgtcca tggggccctt gtcggcggcc agggaggcga
      661 gcaccaccag gggattgcgc atgtcgtgca gcagggtgcc catcaggcgg cggcgcatgc
      721 gcagcgagcg gcggtggccc tccacctggg cggccaggct ttccaggcgg cgcaggcgca
      781 ggcgccaaaa gatccagccg gcggccaaga ggtccggcgc cagcagggcc aggtaggcca
      841 gcacgtagaa ttggccctgg gtccaggccg cgggggagag ccagtaccag cccaccaccg
      901 ccaggtacag ggccagggcg gccagcccca gcaggatggg gccggtgagg gacaccgcga
      961 tcacgaagac cgggatgaca aagagcggcg gggtgaggga gccccggccc cagtaataaa
     1021 aggagggcag gaaggacagg aacagcgagg ccgcgaaggc ccagggcagc gcccgggggg
     1081 cgaaccagcg catccccagg cccaggcgga agagggacag cacggacacg cagcccagga
     1141 tgacgggcca gcgggcgggg atcagcgcca ggttgcgcag ggcccccagc aggaagaaga
     1201 acaggatgcc gttggtgaag agccggacca tgcgggcgcg gaactgctcc agcgggggtt
     1261 cgccgggcag ggaggggtct tcccgctgga ggccga
//
LOCUS       DASTJJ010000051         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_130710, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000051 DASTJJ010000000
VERSION     DASTJJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130710"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VFR02_00600"
     CDS             complement(<1..1275)
                     /locus_tag="VFR02_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868983.1"
                     /translation="MRARPSVFLLWFWLAPLAPVWGGVTYLDLSAAANRGPTDPFDQS
                     FPDAQDSVQKNGLASFPVGDRTFRGIPFRLLDPAKNDGRSFVALKGRERPDFPQAVTL
                     KGPGGGVKDLYFLQTCHWGGTAPHITVAQYEVSYADGQGVTIPLHVGREFTNFWGADD
                     TTESFLAWYYRYKNSTMGVNLFAWKNPRPGQAIQTVVFRSLSKMPVPLLFAVTASSED
                     LPVSPVSPKPEPTAVTDTQGWAAPASGDPSAPPAVDLSGFLDAPAGKHGPVTVKAGSL
                     VFADGTGARFWGTRLLPGVLSMDGAALGDLAARLAADGFNLAEVDLPEGDAPTDAATP
                     APGPPAGLEALLGALKAKGVYLLVKAPEGSSEGASLDDPAVLPEGIFSVLEAAYNPVP
                     TGAPAPVALQNGPQLLGLADSLPAQLILGRAFG"
     gene            complement(1272..2036)
                     /locus_tag="VFR02_00605"
     CDS             complement(1272..2036)
                     /locus_tag="VFR02_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9868984.1"
                     /translation="MPTRRKTRNAPAEHRHASRKFPHAKGPANSNPFIANEFREYDEL
                     NTWRMFKIMSEFVEGFENLRDIRPAVTVFGSARTRPEDPDYELTRKIAYGLSKKGYTI
                     LTGGGPGMMEAANLGAYEAGGKSVGCNIELPFEQKVNSFVNLPVSFHYFFIRKVMFLR
                     YTSAVVVMPGGFGTLDEMFEALTLIQTRKIDPFPVILVDRDYWKKGLEWIRETIMREH
                     QYIDRHDMDLFQVVDGPEEAIRIITDYYRAEKAKKK"
     gene            complement(2060..2497)
                     /locus_tag="VFR02_00610"
     CDS             complement(2060..2497)
                     /locus_tag="VFR02_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9868985.1"
                     /translation="MAHQPQQTPKLAVNALVFNDRGEVLLAKRTDNGLWCIPGGHVDL
                     GETLEQACLRELFEETGLKARVARLVGIYSDPAHSLHIAQGPEWHTVRVSFLCRITGG
                     TITPSEETSEIRYFSPDALPPLITDHAQRVRDGLADAPGAVIG"
BASE COUNT          458 a          871 c          926 g          453 t
ORIGIN      
        1 gccgaacgcg cgccccagga tcagttgggc cggcaggctg tccgccaagc ccaataattg
       61 gggtccgttc tgcagggcca ccggcgcggg ggcgcccgtg gggaccggat tgtaggccgc
      121 ttccaggacc gaaaagatcc cctcgggcaa gacggcggga tcgtccagcg aggcgccctc
      181 ggacgatcct tccggcgcct tcaccaaaag atagacgccc ttggccttga gcgcccccag
      241 gagcgcttcc aacccggccg gggggccggg cgccggggtg gcggcgtccg tgggggcgtc
      301 cccctccggc aggtccacct cggccaggtt gaatccgtcc gcggccaaac gggccgccag
      361 gtcccccaac gccgccccgt ccatggacag gacccccgga agaagccggg tcccccagaa
      421 acgggcgccc gtcccgtcgg cgaagaccaa gcttccggcc ttcaccgtga cgggcccgtg
      481 tttcccggcg ggcgcgtcca ggaaaccgct caggtccacc gcggggggcg cggaagggtc
      541 acccgacgcg ggcgccgccc aaccctgggt gtcggtcacg gccgtgggct cgggcttggg
      601 ggaaacaggc gagaccggca ggtcctcgct ggaggccgtc acggcgaaca acagcggcac
      661 cggcatcttg gacagggacc tgaagacgac ggtttggatg gcctgcccgg gccgggggtt
      721 cttccaggcg aagaggttca cgcccatggt ggaattttta taacggtagt accaggccag
      781 gaagctctcg gtggtgtcgt ccgcgcccca gaagttggtg aattcccggc ccacatgcag
      841 ggggatggtg acgccctgtc cgtcggcgta cgacacctcg tactgggcca cggtgatatg
      901 cggtgccgtg cccccccaat ggcaggtctg caggaagtag aggtccttca ccccgccgcc
      961 ggggcccttc agggtgaccg cctgggggaa atcaggccgc tcccggccct tcagcgccac
     1021 gaaggaccgg ccgtcgttct tggccggatc cagcagtcgg aaggggatgc cgcggaaggt
     1081 ccggtcgccc accgggaagg aggccaggcc gttcttttgc acgctgtcct gggcgtccgg
     1141 gaaggattgg tcgaaggggt ccgtggggcc ccggttggcg gcggccgaca ggtccaggta
     1201 ggtcacgccg ccccaaaccg gcgccagcgg ggccaaccag aaccagagaa ggaagacgga
     1261 cggccgggcc ctcatttttt cttggctttt tcggcccggt aataatccgt gatgatgcgg
     1321 atggcctcct cggggccgtc caccacctgg aagaggtcca tgtcgtgccg gtcgatgtac
     1381 tggtgctccc gcatgatggt ctcccggatc cattccaacc cttttttcca atagtcccgg
     1441 tccaccagga tcaccgggaa ggggtcgatc ttgcgggtct ggatgagggt cagggcttcg
     1501 aacatctcgt ccagggtgcc gaaaccgccg ggcatgacca ccaccgccga ggtatagcgc
     1561 aggaacatga ccttgcggat gaagaaataa tggaagctca ccggcaggtt cacgaaagag
     1621 ttcaccttct gctcgaaggg cagctcgatg ttgcagccca ccgacttgcc ccccgcctca
     1681 taggcgccca ggttggccgc ctccatcatg ccaggccccc cgccggtcag gatggtgtag
     1741 cccttcttgg agagcccata ggcgatcttg cgggtcaact cgtaatccgg gtcctccggg
     1801 cgggtgcggg ccgaaccgaa gacggtcacc gccggacgga tgtcccggag gttctcgaac
     1861 ccctccacga actcggacat gatcttgaac atccgccagg tgttgagctc atcgtactcg
     1921 cggaactcgt tggcgatgaa ggggttgctg ttggccgggc ccttcgcgtg ggggaatttc
     1981 cgggaagcgt ggcggtgttc ggccggggcg ttccgtgtct ttcggcgggt cggcatgggg
     2041 tctcctcggg ttggggatgt cagccaatca cggcgccggg cgcgtccgcg agcccgtccc
     2101 ggacgcgctg ggcgtggtcc gtgatgaggg gcggcagggc gtccggggaa aaatagcgga
     2161 tctcgctggt ttcctcgctg ggggtgattg taccaccggt gatccggcac aaaaaggaga
     2221 cccggaccgt gtgccattcg gggccctggg cgatgtgcag cgaatgggcc gggtccgaat
     2281 agatgcccac cagccgcgcc acccgggcct tgagcccggt ctcctcgaac agttcccgca
     2341 ggcaggcctg ttccagggtc tcgcccaggt ccacgtggcc gcccgggatg caccaaaggc
     2401 cgttgtccgt gcgcttggcc aacagcactt ccccgcggtc gttgaagacc agcgcgttca
     2461 cggccaactt gggcgtctgc tggggttgat gggccatcgt tcctccggaa gagacactct
     2521 tctccgcaga gacgcagagg aaaagcggat gacatccacg ggctccaaca ggacttcacc
     2581 gcgtctctgc gcctctgtgg tgaaaaatcc gtcctttgcc gtaaaacaaa aaatcccacc
     2641 ccccgaagcc gggggatggg acggcgaggc tttttcgtta acgcttcgag aactggaagc
     2701 ggcggcgg
//
LOCUS       DASTJJ010000052         1160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1109178, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000052 DASTJJ010000000
VERSION     DASTJJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109178"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..>1160
                     /locus_tag="VFR02_00615"
     CDS             106..>1160
                     /locus_tag="VFR02_00615"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HET9868986.1"
                     /translation="MDPRRLFPALACLSALAFGLGGCSSIRTNLYIPPSEEAARPFPD
                     PPPAVIRLPVSVPMPQLAAIPKNTLRWVEDQLAGIGENAQKQEGLVRLPGPLLGVDRL
                     WNALQEPIYIDKNIWLLIRPESLSSGLEQPERLDPFTWKVVLEMTARPLLIFGGKPEW
                     RKKNLPPLKPYKPGPTGFHAVSNAFISFKEANRILADPKTGLVDYPIRGSGSYHLRVK
                     GVRLYGSGGQVIAQTRIEYHPLINFDGKPSRMTIYFRGTPRYDPKREVFYLHRLDFDV
                     RTGDLLMQVASWIFKSDILNALRRKAHIPIGSELDKLKERMNVVLNRPVGEHHLLRTQ
                     VQSFRVLDARVHPEGIEA"
BASE COUNT          210 a          401 c          343 g          206 t
ORIGIN      
        1 ccatggtcgg tctccttggg gtcgggggcg cttatcttgc cagaagcggg ggaaaagctc
       61 aatcttttag gcccccgcgc cgtgccctgg atttatagta aagccatgga cccgcgccgc
      121 ctttttcccg cactcgcctg cctttcggcc ctcgccttcg ggctcggcgg ctgttcctcc
      181 atccgtacca acctctacat cccgccctcg gaggaggccg cccggccctt ccccgacccg
      241 cctccggccg tcatccgcct gccggtgtcc gtccccatgc cccagctggc cgccatccca
      301 aagaacaccc tgcgttgggt cgaggaccag ttggcgggga tcggggagaa cgcccagaaa
      361 caggaaggcc tggtccgcct gccggggccg ctcttgggag tggaccggct ctggaacgcc
      421 ttgcaggagc ccatctatat cgacaagaac atctggctgc tcatccggcc cgaaagcttg
      481 agttcgggcc tggaacagcc cgagcgcctg gaccccttca cctggaaggt ggtgctggag
      541 atgaccgccc gtcccctgtt gatcttcggg ggaaaaccgg aatggaggaa aaaaaatctg
      601 cctcccctca agccctacaa gccgggcccc acggggttcc acgcggtcag caacgccttc
      661 atcagcttca aggaagccaa ccgcatcctg gccgacccca agaccggcct ggtggattac
      721 cccatccggg gcagcggcag ctaccacttg cgggtgaagg gggtgcggct ctacgggtcg
      781 ggcgggcagg tcatcgccca gacccgcatc gaataccatc ccctcatcaa tttcgacggg
      841 aagccttccc gcatgaccat ctatttccgg ggcacgcccc gctacgaccc caaacgggag
      901 gtcttctacc tgcaccggct ggatttcgac gtgcggacgg gggacctttt gatgcaggtg
      961 gccagttgga tcttcaaatc ggacatcctc aacgcgctca ggcgcaaggc ccatatcccc
     1021 atcgggtccg agttggacaa gctcaaggaa cgcatgaacg tggtcttgaa ccggcccgtc
     1081 ggggaacacc acctgctgcg cacgcaggtc caatccttcc gggtgctgga cgcccgggtg
     1141 catccggaag gcatcgaggc
//
LOCUS       DASTJJ010000053         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_497559, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000053 DASTJJ010000000
VERSION     DASTJJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497559"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="rplU"
                     /locus_tag="VFR02_00620"
     CDS             <1..361
                     /gene="rplU"
                     /locus_tag="VFR02_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET9868987.1"
                     /translation="LQPGGWGFHHLRRQCMYAVIQTGGKQYRVSEGDVITVDRVQGEP
                     GDSIVFGKVILLENAGKVQVVAKEIQNAKVTGKILGNKSGKKIRVFRYRRRKNTMRTK
                     GSRPKYTSISIEKITTA"
     gene            380..640
                     /gene="rpmA"
                     /locus_tag="VFR02_00625"
     CDS             380..640
                     /gene="rpmA"
                     /locus_tag="VFR02_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9868988.1"
                     /translation="MGQHKAGGSTRNGRDSNSQRLGIKAFGGNFVTAGSILVRQRGMR
                     YKPGHNVGVGKDDTLFATVSGLVTFREFKKGGKMISVEPAQA"
     gene            755..>1905
                     /gene="obgE"
                     /locus_tag="VFR02_00630"
     CDS             755..>1905
                     /gene="obgE"
                     /locus_tag="VFR02_00630"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET9868989.1"
                     /translation="MIIDEVTITLEAGAGGNGCLSFRREKFIPRGGPDGGDGGRGGSI
                     YLEARLDSHTLVDFTYRPLFEARHGGHGKGKNMHGKDAEDLVVAVPVGTMVFDADKRL
                     VVDMDQAGKRFLIAKGGRGGRGNTTFTSAVRQAPRLAEKGEPGEKKTVRLELKLLADV
                     GLVGFPNAGKSTLLSRVTRARPKIADYPFTTLEPHLGVVEMGGNRQFVLADVPGLIED
                     ASEGRGLGIRFLKHLERTQMLLHLVEIAGVETQAELSAKVRTIEKELKKYGGRLAQLP
                     RILVLTKVDAVGDEAKPARWKAALEKKQGPVFVISAVSGRGLKELLEEVWKRLEALWK
                     AEREKPVVVEPAVFQSKERFRVEKDGASFRVGGPEIDKWVAMTNFNSRDA"
BASE COUNT          377 a          562 c          674 g          292 t
ORIGIN      
        1 tctccaacct ggtggttggg gttttcatca tttgaggagg cagtgcatgt acgccgtgat
       61 ccagacgggt ggcaaacaat accgcgtttc tgaaggggat gtgatcaccg tggaccgggt
      121 ccagggtgag ccgggcgatt ccatcgtgtt cggcaaggtc atcctgttgg agaacgccgg
      181 gaaggtgcag gtggtggcca aggagatcca gaacgccaag gtgacgggca agatcctggg
      241 caacaagtcg ggcaagaaga tccgcgtctt ccgttaccgc cgccgcaaga acaccatgcg
      301 gaccaagggc tcgcgcccca agtacacgtc catctccatc gaaaaaatca ccacggctta
      361 acagtccagg aggcgaatca tgggtcagca taaagcgggt ggttccaccc ggaacggtcg
      421 ggacagcaac agccagcggt tgggcatcaa ggcattcggc ggcaatttcg tgacggcggg
      481 gagcatcctg gtccgtcagc gcggcatgcg ttacaagccc ggccacaacg tcggcgtggg
      541 caaggacgac acccttttcg cgacggtgtc gggcctggtc accttccggg agttcaagaa
      601 gggcggcaag atgatcagcg tggagccggc gcaagcctga tccccgcggt ggcatcggaa
      661 ccccgcccga ccgggcgggg tttttttatt tccggacaag gtccatccca ttccatcccg
      721 gggcatcccc cgggcaaggg acattgaaga cgccatgatc atcgacgaag tcaccatcac
      781 attggaggcg ggggcgggcg gcaacggctg cctgagcttc cgccgcgaga aattcatccc
      841 ccggggcggc ccggacgggg gcgacggcgg ccggggcggc agcatctacc tggaggcccg
      901 gctggattcc cataccctgg tggatttcac ctaccgcccc ctcttcgagg cgcgccacgg
      961 cggccacggc aagggcaaga acatgcatgg gaaggacgcc gaggacctgg tggtggccgt
     1021 gcccgtaggc accatggtgt tcgacgcgga caagcgcctg gtggtggaca tggaccaggc
     1081 ggggaagcga ttcctcatcg ccaagggcgg ccggggcggc cggggcaaca ccaccttcac
     1141 cagcgcggtg cgccaggcgc cccgcctggc cgagaaaggg gaaccgggcg agaaaaagac
     1201 cgtgcgcctg gagctcaagc tgctggccga cgtggggctc gtgggtttcc ccaacgcggg
     1261 caagtccacc ctgctgtccc gggtgacccg ggcgaggccc aagatcgccg attatccctt
     1321 caccaccctg gagccgcacc tgggcgtggt ggagatgggc ggcaaccggc agttcgtgct
     1381 ggcggacgtg ccgggtctga tcgaagacgc cagtgagggc cggggcttgg gcatccgttt
     1441 cctcaagcac ctggaacgca cccagatgct gctgcacctg gtggagatcg ccggggtgga
     1501 gacccaggcg gaactttcgg ccaaggtcag gaccatcgag aaggaactga agaaatacgg
     1561 gggccgtctg gcccaattgc cccggatcct ggtgctgacc aaggtggacg ccgtgggcga
     1621 cgaggcgaag cccgcccgct ggaaggccgc cctggaaaaa aaacaggggc cggtcttcgt
     1681 gatctcggcg gtctcgggcc gggggctgaa ggaactgctg gaggaggtct ggaagcggtt
     1741 ggaggcgctt tggaaggccg agcgcgaaaa acccgtcgtg gtggaacccg cggtcttcca
     1801 gtccaaggaa cggttccggg tggaaaagga cggggcgtcc ttccgggtgg gcgggcccga
     1861 gatcgacaaa tgggtggcca tgaccaattt caacagccgg gacgc
//
LOCUS       DASTJJ010000054         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_742285, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000054 DASTJJ010000000
VERSION     DASTJJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742285"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..669)
                     /locus_tag="VFR02_00635"
     CDS             complement(16..669)
                     /locus_tag="VFR02_00635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9868990.1"
                     /translation="MPALPPPRFLTRYFPGFSKKDLSALQDYYQSQLKGHATPEKRVG
                     WQTAHSQRVRFEALACVGDLRGKTILDVGCGLGGFYAYLRDRRLGVRYRGVDLFPPVI
                     AEAKEAHPGARFEARNIYERPFPRGAFEYAFFSGVFNVRVKDNWRYLRALLKGALGQV
                     RKGLAFNVLNAEAGLKESDRFFVHPKELAAFGRRLGGSRVRLLDHYHHLDLTLHIYK"
     gene            complement(679..1512)
                     /locus_tag="VFR02_00640"
     CDS             complement(679..1512)
                     /locus_tag="VFR02_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9868991.1"
                     /translation="MPTFDFCIEAPGGRDLWGTVTAPQRPQGPPVVMAHGFLGFKDWA
                     FFPWLAETFAREGFPAVRFNFSGSGMGPEADGPFRQLEAFQADTISRQVEDLHRVLRA
                     VTGGQFAREGLAGGDSALLWGHSRGGAVCLLGAVNRPEVKGVSTWASISRVNRYFYED
                     KKAWRERGFFPVESARTGQQLRYSPDFLDDVERWGKAGDVPTHLHHLTVPVLLVHGSE
                     DDSVPPEESESLAALYPQSRLAILAGANHKFNASHPFAEAAPVLREAAQRTVEFYRTL
                     G"
     gene            complement(1677..>1839)
                     /locus_tag="VFR02_00645"
     CDS             complement(1677..>1839)
                     /locus_tag="VFR02_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HET9868992.1"
                     /translation="SVVDFTIIAFSVFLLVKLLNSLQRKKQAEAAAAPPEPSPEVKLL
                     TEIRDLLKR"
BASE COUNT          328 a          601 c          615 g          295 t
ORIGIN      
        1 cggccgggct cgggcctact tataaatatg aagggtcagg tccaggtggt ggtaatggtc
       61 cagcagccgg acccgggagc cgcccaggcg gcgcccgaag gccgccaatt ccttcggatg
      121 gacgaagaag cggtcgcttt ccttcaggcc cgcctcggcg ttcaggacgt tgaaggccag
      181 ccccttcctc acttggccca gggcgccctt cagcagggcc cgcaggtagc gccagttgtc
      241 cttgacgcgc acgttgaaga cgccggaaaa gaaggcgtac tcgaaggcgc cccggggaaa
      301 gggcctttca taaatgttgc gggcctcgaa gcgggcgccc ggatgggcct ccttcgcctc
      361 ggcgatgacc ggggggaaca ggtccacgcc cctataacgc acccccaggc ggcggtcccg
      421 caggtaggcg taaaagcccc ccaggccgca acccacgtcc aggatcgtct tgcccctcag
      481 gtcgcccacg caggccagcg cctcgaaccg cacccgctgg ctgtgggcgg tctgccagcc
      541 cacgcgtttt tcgggggtgg cgtgtccctt gagctgggac tggtaatagt cctggagcgc
      601 cgaaaggtcc ttcttggaaa aacccgggaa gtagcgggtc aggaaacggg gcgggggcaa
      661 ggcgggcatg cccttaactt acccgagggt gcggtaaaac tccaccgtcc gctgggcggc
      721 ctcccgaagc accggggcgg cttcggcgaa ggggtgggag gcgttgaact tgtggttggc
      781 cccggccagg atggccaggc gggattgggg gtagagggcg gccaggctct cggattcctc
      841 gggcggcacg ctgtcgtcct ccgagccgtg gaccagcagg accggcacgg tcaggtggtg
      901 caggtgggtg ggcacgtcgc ccgccttgcc ccagcgttcc acgtcgtcca ggaagtcggg
      961 cgagtaacgc agttgctggc ccgttcgggc gctttccacg gggaaaaacc cccgttcccg
     1021 ccaggccttc ttgtcctcgt aaaagtagcg gttgacccgg gagatggagg cccaggtgga
     1081 cacccccttc acctcgggcc ggttcaccgc ccccaagagg cagaccgcgc cgccccgcga
     1141 atggccccag agcagggccg agtccccccc cgcgagcccc tcccgggcga actgcccgcc
     1201 ggtgaccgcc cgcagcacgc ggtgcaggtc ctccacctgg cgggaaatgg tgtcggcctg
     1261 gaaggcctcc aactggcgga aaggcccgtc cgcttccggg cccatgccgg agcccgagaa
     1321 gttgaagcgc accgccggaa agccctcgcg ggcgaaggtc tcggccagcc aggggaagaa
     1381 ggcccaatcc ttgaacccca ggaacccgtg ggccatgacc accggcgggc cctggggcct
     1441 ttggggcgcg gtcaccgtgc cccagaggtc ccgccccccc ggcgcctcga tgcagaagtc
     1501 gaaagtcggc actggccccc ctttttccgg tttgacgtcc aatttcggaa ccacgtcttc
     1561 gacaaaacgg caaagttgga cgttccgaaa caatgataaa cattgatgga acctgatgcc
     1621 cgaaaataaa aatcaggctt tatcaggctt catcaatatc ccaatgggct ttgcttctat
     1681 cttttcagca ggtcgcggat ctcggtgagg agcttcacct cgggcgacgg ctcgggcggc
     1741 gcggcggcgg cttcggcctg cttcttgcgc tgcaggctgt tgaggagctt caccagcagg
     1801 aagaccgaga aggcgatgat ggtgaagtcc accaccgat
//
LOCUS       DASTJJ010000055         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_497690, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000055 DASTJJ010000000
VERSION     DASTJJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497690"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFR02_00650"
     CDS             <1..692
                     /locus_tag="VFR02_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9868993.1"
                     /translation="RLPFIFMNSVGLDGDMRTLLHEGGHAMHSFASRGIPFPPYRHAP
                     MEFCEVASMSMELLADPHLGVFYPSPADAARSIRKHLEDVVTLLPWIATVDAFQHWIY
                     THPDHSVKDRGDLWVGLRERLGGMEDWDGFEPVRRSLWHRQLHIFEVPFYYIEYGIAQ
                     LGALQVWKNARQKGAEGVAAYKRGLSVGGSLTLPEIFRSAGIRFDFSRDMIEPLMDEV
                     HRELARLEKLS"
     gene            complement(685..1437)
                     /locus_tag="VFR02_00655"
     CDS             complement(685..1437)
                     /locus_tag="VFR02_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9868994.1"
                     /translation="MDLPFRSKGLGLKALAFLLAVSPLNAAPLYESPDPDVTLAHLPD
                     KVWTDLGGLFTPDQALVAGLGGSLTAADWVFFDPSNPWPQDLRAWGTPGLWDFGDFYG
                     QGWVEGFGGVGCWAWGGLTGDHRLACFGRDASESLAMATLLVTGLKYAVGRERPDGSN
                     HFSFPSGHTITAFCVAPVVAKYGGWELGVPAYALAAVTGLSRVEGEHHYLSDVLAGAT
                     LGILLGDLVVERPKDLSLSPTPGGAQVTWSFN"
     gene            complement(1440..>1632)
                     /locus_tag="VFR02_00660"
     CDS             complement(1440..>1632)
                     /locus_tag="VFR02_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HET9868995.1"
                     /translation="DPPIDRKRLIGAFVFGAGWALAGICPGTVFAQLGEGKWSALFAL
                     AGLVAGVWAYLQFGPRSKK"
BASE COUNT          307 a          540 c          534 g          251 t
ORIGIN      
        1 tgcggctccc cttcatcttc atgaactcgg tgggcctgga cggcgacatg cgcaccctgc
       61 tgcatgaggg cgggcacgcc atgcacagtt tcgcgtcccg gggcatcccc ttccccccct
      121 accggcacgc gccgatggaa ttctgcgagg tggcgtccat gtccatggag ctcctggccg
      181 acccccacct gggggtcttt tacccctcgc ccgccgacgc ggcccgttcc atccgaaagc
      241 acctggagga cgtggtcacc ctgcttccct ggatcgccac ggtggacgcc ttccagcact
      301 ggatctatac ccatccggac cactcggtga aggaccgtgg cgatctttgg gtcggattga
      361 gggaacggct gggcggcatg gaggattggg acgggttcga gccggtgagg cgcagcctct
      421 ggcaccggca attgcacatc ttcgaggtgc ccttctatta catcgaatac gggatcgccc
      481 aactgggggc cttgcaggtc tggaagaacg cccggcaaaa gggggccgaa ggcgtggcgg
      541 cctacaaacg gggcttgagc gtgggaggga gcctgaccct tccggagatc ttccggtcgg
      601 ccggcatccg tttcgatttc agccgggaca tgatcgagcc cctcatggac gaggtgcacc
      661 gggaattggc ccgcctggaa aagctcagtt aaaggaccag gtcacctggg ccccgcccgg
      721 ggtcggggac agcgacaggt ccttggggcg ctccaccacc aagtccccca gcaaaatgcc
      781 cagggtggcc cccgccaaca cgtcggacag gtagtggtgt tccccttcca cccgggaaag
      841 gccggtcacc gcggccaggg cataggccgg gacgcccagt tcccaaccgc cgtacttggc
      901 caccacgggt gccacgcaaa aagccgtgat ggtatggccc gaggggaagg aaaaatggtt
      961 gctgccgtcg ggacgctcgc gccccacggc gtatttcaag cctgtcacca gcagggtggc
     1021 catggccagg gattcgctgg cgtcccggcc gaaacaagcc aaccggtggt cccccgtcaa
     1081 tcccccccag gcccagcagc ccacgccccc aaagccttcg acccatcctt ggccgtagaa
     1141 atccccgaag tcccaaagcc cgggcgtgcc ccaggccctc aagtcttgcg gccagggatt
     1201 cgagggatcg aaaaagaccc agtcggcggc ggtcagggaa ccgcccaggc cggccaccag
     1261 ggcctggtcg ggggtgaaaa ggccgcccag atcggtccaa accttgtcgg ggagatgggc
     1321 cagggtgacg tccggatcgg gcgattcata gagaggggcc gcgttcaagg gcgagaccgc
     1381 cagcaggaag gccagggcct tgaggcccag gcctttcgat cgaaagggaa ggtccaagat
     1441 tatttcttgg atctgggtcc gaattggaga taggcccaga ccccggccac caaccccgcc
     1501 agggcgaaaa gcgcgctcca cttgccctcg cccaattggg cgaagaccgt gccggggcag
     1561 atgcccgcca aggcccagcc ggccccgaag acaaaggccc cgatgaggcg tttgcggtcg
     1621 atcggggggt cg
//
LOCUS       DASTJJ010000056         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1721530, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000056 DASTJJ010000000
VERSION     DASTJJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721530"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFR02_00665"
     CDS             <1..228
                     /locus_tag="VFR02_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868996.1"
                     /translation="NSAIIPLMVGDNMKTFAMTKELGEMGVFATPVVSPAVPSDKTLI
                     RTSYTSTHTDAQLDQVLESFRKVGKKYGVIS"
     gene            301..1455
                     /locus_tag="VFR02_00670"
     CDS             301..1455
                     /locus_tag="VFR02_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET9868997.1"
                     /translation="MAVTVKPIDPRNKKQREAFLHLPWKIYRTADGKKDPHWVPPFLD
                     DQRSLLDPDKNPFWKHAKVALYAAYDEKGEIVGRISASVDDNFNQFWDAKVGFFGWFE
                     CINDPAVAQALFNEAAGFLKAEGMTVMRGPSSFTSNDDYFGFLLEGHDSPARIGMTYN
                     PPYYLTLSEGSGLAKVKDLYAWYLSAQIDLPERIVKIAERTKQRERITIRPLNMKRLF
                     EDAAILKDLYNKIWEKNWGFVPMTDEEFNYQVKKLKDVVWPDFVVFAEVDGKPIGFNL
                     VVPDVNQLLIKMNGELFSKEAPFALFTFLFGLNKIDDTREMAMGVLPEYRKKGLEAIL
                     YLEALKTGKKRRIKGGELSWTLEDNEGINNGIEAMGGKIIKKYRLYEKAL"
     gene            complement(1460..1744)
                     /locus_tag="VFR02_00675"
     CDS             complement(1460..1744)
                     /locus_tag="VFR02_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868998.1"
                     /translation="MRIPIQNQALAQAAAEAAQDRRIDLARGREAGEKAKVDGALRDS
                     EKKKTALGGEKTGDGNGQSAPGYRKPGSPPGEARGHEDGPPARGRIDIRV"
     gene            1873..>2673
                     /locus_tag="VFR02_00680"
     CDS             1873..>2673
                     /locus_tag="VFR02_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9868999.1"
                     /translation="MKKTVLSALILACLASLPVFFGCSPNQSPTGFFTSNTPTPSQGA
                     SIVFSRSECGDLLAGNDLSVTVCSVICNQSPSTSRPLTLIENSLGAGFQFQGFGWNGT
                     FPYVDPSVGADPRFTSPNRLSFPNGLAASACVTVLSVFENPDWPANACQTAVADAGVS
                     LSGLPLLSSSVTLQVPCATDTPVFTPTRTPTQTVTITAGPSRTPTPTLTSTFPVTLTG
                     TPTATRTATVSPTASPSTTPSPAFTATASYSPTPTPTGCAKIQLSLSAP"
BASE COUNT          506 a          976 c          711 g          480 t
ORIGIN      
        1 aactcggcca tcatccccct gatggtgggc gacaacatga agaccttcgc catgaccaag
       61 gagttggggg aaatgggcgt cttcgccacc ccggtggtct cccccgccgt gccgtcggac
      121 aagaccctca tccgcacttc ctacacctcc acccacaccg acgcgcagct ggaccaggtg
      181 ctggaaagct tccggaaggt cggtaagaaa tacggcgtca tttcctgacc ccggccggaa
      241 acccccgcgt ccccgggatc gtccaaacac caacacccaa ccctcttaaa ggaggctgag
      301 atggccgtca ccgtgaaacc gatcgatccc cggaacaaaa agcaacggga ggccttcctc
      361 cacctcccct ggaagatcta ccggacggcc gacggcaaga aggaccccca ctgggtccct
      421 cccttcctgg acgaccagcg cagcctgctc gaccccgaca agaacccctt ctggaagcac
      481 gccaaggtgg ccctctatgc cgcctacgac gaaaagggcg agatcgtcgg aaggatctcg
      541 gccagcgtgg acgacaactt caaccagttt tgggacgcga aggtggggtt tttcggctgg
      601 ttcgaatgca tcaacgaccc ggcggtcgcc caggccctct tcaacgaagc cgccggcttc
      661 ctcaaggccg aaggcatgac ggtcatgcgg gggccctcca gtttcacttc caacgacgac
      721 tatttcggtt ttctgctcga aggccatgat tcccccgccc gcatcggcat gacctacaac
      781 ccgccctact acttgacgtt gtccgagggc tccgggctgg cgaaggtcaa ggacctctac
      841 gcctggtacc tgagcgccca gatcgacctg cccgagcgca tcgtcaagat cgccgagcgc
      901 accaagcagc gcgagcgcat caccatccgc cccctgaaca tgaagcgcct tttcgaggac
      961 gccgccatcc tgaaggacct ctacaacaag atctgggaaa agaactgggg cttcgtaccc
     1021 atgaccgacg aggagttcaa ctaccaggtc aagaaactca aggacgtcgt ctggcccgac
     1081 ttcgtggtct tcgccgaggt ggacggcaaa cccatcggct tcaatttggt ggtccccgac
     1141 gtgaaccagt tgctcatcaa gatgaacggc gagttgttct cgaaggaagc ccccttcgcg
     1201 ctcttcacct tcctcttcgg gctcaacaag atcgacgaca cccgggaaat ggccatgggc
     1261 gtcctgcccg aataccgtaa aaaggggctg gaggccatcc tctacctgga ggcgctcaag
     1321 acgggtaaaa aacgcaggat caagggcggc gaactctcct ggaccctgga ggacaacgaa
     1381 ggcatcaaca acggcatcga ggccatgggc gggaagatca tcaagaagta ccgcttgtac
     1441 gaaaaggccc tctgaacccc tagacccgga tgtcgatgcg cccccgggcg ggcgggccgt
     1501 cttcatgccc ccgggcttcc cccggcgggg acccgggctt ccggtagccg ggagcgcttt
     1561 ggccgttgcc gtcccccgtt ttttcacccc ccaaggcggt ctttttcttt tccgagtccc
     1621 gcagcgcccc atcgaccttg gccttttccc ccgcttcccg gccccgggcg aggtcgatcc
     1681 gccggtcctg ggcggcctca gcggcggcct gggccaaggc ctggttctgg atggggatcc
     1741 gcatcggcgg atcgttctgg attccctgca tggccgccct cccggcaaat gtccaaacaa
     1801 tcaagggaat aacccattcc cccggaccac taggatatcg gggaatatcc ttgggtggaa
     1861 tgggggaaaa agatgaaaaa aacggtcctt tcggcgctga tcctggcctg cctggcctcg
     1921 ctcccggtct ttttcgggtg cagtcccaac cagagtccga ccggcttctt tacttcgaac
     1981 actcccaccc cttcccaagg cgcctccatc gttttttccc gttccgaatg cggggatctc
     2041 ctcgcgggca acgacttgtc cgtcacggtc tgcagcgtga tctgcaacca gtccccttcc
     2101 acttcccgac ccctgacgct catcgagaac tccttgggag ccggtttcca gttccagggt
     2161 ttcggctgga acgggacctt cccttatgtg gatccctccg tcggggccga tccccgcttc
     2221 acctccccca accggctctc ctttcccaat gggctggcgg cctcggcctg cgtgacggtg
     2281 ctctcggtct tcgagaatcc tgattggccc gccaacgcct gccaaaccgc cgtcgccgac
     2341 gcgggggttt ccctctcggg ccttcccctg ctcagttcct cggtgaccct ccaggtccct
     2401 tgcgccaccg acaccccggt cttcaccccc acccggacgc ccacccaaac ggtgaccatc
     2461 accgccggtc ccagccggac gcccacgccc accctcacca gcaccttccc cgtcaccctg
     2521 accgggaccc ccacggccac ccggaccgcg acggtgagtc ccaccgccag cccctccacg
     2581 acccccagtc cggccttcac ggccaccgcc tcctatagtc cgacccccac tccgacgggt
     2641 tgcgccaaga tccaactgtc cctctccgcc ccc
//
LOCUS       DASTJJ010000057         1718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1231352, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000057 DASTJJ010000000
VERSION     DASTJJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231352"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1718)
                     /locus_tag="VFR02_00685"
     CDS             complement(<1..>1718)
                     /locus_tag="VFR02_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869000.1"
                     /translation="GLDYTSGTAQCGQPVTVNMPPNAIPVTAFLYVEGYGSGYPTGTP
                     APPLTGSTATFNGSGTGVGAAVQVGSPMNFDYYQSWDGARFGLDPSRITGIGGGPNQL
                     KYNASFSLTNGYSCRTWTLVVLYDDPSVTTMGVVDLSDGNQVWHIEDDGGNPGAVSIK
                     YGQAPTDAKQDWNCAGVACANSKTHFTAIGGDDGCGHTADPDELVPYGSGGVTGAGGP
                     PYFQSPYFILDCSTGPDQVAKKRDYYQGSDYQSNAVSGQQLEWAMPLLYSLSKTTYWQ
                     QVLVTQMSCPVVPQCSVTWDHSQGTFYGAPPGWVTGMVPVTQGTNWVQSGGYISRNNS
                     CGSYNSSQLLQNQVQSGPGTLLVELQAGSQTDEGLLFNYNRGTGSGYALHFTYNGCNP
                     GNTIVFGKYTNGTFASLSSAALPNILCNPIWVQLVINGCNYTVSVGTAKTSLSVVTTF
                     TDCTYTSGMLGYDGGHCGSYKYYYFQWNGICNTPTPTPTPTKTPTFTPTPTPTITKTP
                     TPTVTPTQTPTITPTITPTVTPTITPTITPTVTPTVTLTVTTTSTATVTRTASATSTA
                     TSIPDTA"
BASE COUNT          274 a          492 c          614 g          338 t
ORIGIN      
        1 gccgtgtcgg ggatcgaggt ggccgtggag gtcgcgctgg ccgtacgggt cacggtcgcc
       61 gtggaagtgg tggtcacggt cagggtgacc gtcggggtca ccgtcggcgt gatggtcggg
      121 gtgatggtgg gcgtcaccgt cggggtgatg gtcggcgtga tcgtgggggt ctgggtgggg
      181 gtcacggtgg gcgtgggcgt tttggtgatg gtgggggtcg gggtgggcgt gaaggtcggc
      241 gtcttggtcg gggtgggggt cggggtgggc gtgttgcaga tgccgttcca ctggaagtaa
      301 tagtatttat aggaaccgca atggccgccg tcatagccca acatgccgct ggtataagtg
      361 cagtccgtga aggtggtgac gacggaaaga ctggtcttgg ccgtccccac gctcacggtg
      421 tagttacagc cgttgatgac caactgcacc cagatcgggt tgcaaaggat gttcggcagg
      481 gccgccgagc tcagcgaggc gaaggtgccg ttggtgtact tgccgaagac gatggtgttg
      541 cccgggttgc aaccgttgta ggtgaagtgg agcgcgtacc cgctgcccgt gccgcggttg
      601 tagttgaaca ggagcccctc gtcggtctgg gaacccgcct gcagttccac caacagggtg
      661 cccgggccgc tctggacctg gttctgcagc aactgggaag aattgtagct gccgcaggaa
      721 ttgttccggc tgatgtagcc gccgctctgc acccagttgg tcccctgggt gaccggcacc
      781 atgcccgtga cccatcccgg aggcgcgccg tagaaggtcc cctggctgtg gtcccaggtg
      841 accgagcact ggggcaccac cgggcaggac atctgggtga ccagcacctg ctgccaatag
      901 gtggtcttgc tcaagctgta gagcaggggc atggcccatt ccagctgctg tcccgacacc
      961 gcgttgctct ggtaatccga cccctggtaa taatcccgtt tcttggccac ttggtccggg
     1021 cccgtggagc aatccaggat gaaatagggg ctttggaagt aaggcggacc gccggcgccc
     1081 gtcaccccgc ccgaaccata gggtaccaat tcgtccgggt cggccgtatg gccgcaaccg
     1141 tcgtccccgc cgatggcggt gaagtgggtc ttggaattgg cgcaagccac gcccgcgcag
     1201 ttccagtctt gtttggcgtc ggtgggcgct tggccgtatt tgatcgacac ggcccccggg
     1261 ttgccgccgt cgtcctcgat gtgccagacc tggttcccgt ccgaaaggtc caccacgccc
     1321 atggtggtca ccgagggatc gtcataaagc accaccaggg tccaggtccg gcacgaataa
     1381 ccgttggtca ggctgaagga ggcgttgtac ttgagctggt tgggcccgcc cccgatgccc
     1441 gtgatgcggc tgggatccaa cccgaaccgc gcgccgtccc agctttggta atagtcgaaa
     1501 ttcatgggcg agcccacctg cactgcggcg cccactcccg tgccgctgcc gttgaaggtg
     1561 gccgtgctgc ccgtcagcgg cggcgccggg gttcccgtgg gataaccgct cccgtaacct
     1621 tccacataaa ggaaggcagt aacggggatg gcgttggggg gcatattgac cgtgacgggc
     1681 tggccgcatt gggccgtgcc cgaggtgtaa tccaaccc
//
LOCUS       DASTJJ010000058         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_131450, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000058 DASTJJ010000000
VERSION     DASTJJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131450"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFR02_00690"
     CDS             <1..719
                     /locus_tag="VFR02_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9869001.1"
                     /translation="LVWTLFSGSPVRVGFTDQRQGHPSGKIYNLGVPLCPEDLQERDK
                     NWKLVERLFPGMGKPPALRCPALPAGTERKVAAFYRSAGIGPRDRVLGLHPTLGKADN
                     RWSQKKYLELLRRLSRKGGFKFVLVHGWGEGEALARFQAMAARVPGVTALPETDLFFI
                     LGAARRFAGLVCNDSGLMHALSLVTRVAAVFGPSEPSRWGPIGPHAHRIFRAADRRCD
                     SVRPADVARYVQSRWRGRPS"
     gene            737..>1504
                     /locus_tag="VFR02_00695"
     CDS             737..>1504
                     /locus_tag="VFR02_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HET9869002.1"
                     /translation="MGLFDQFKKGLQKTSQSLWGGLTGLLQGEALSEADLARLEEGLI
                     LADVGPATARALAAEVREKSRRSPQATAGLLRESLLERLSKVQSPLELPKPSVILLLG
                     VNGAGKTTTAGKLALRFAQEGRRVLLAAGDTFRAAAEEQLTEWAGRAKVDLFCGKHGA
                     APSAVVFDAVQAGISRGADLVLVDTAGRLHNRPNLMEELEKIVRVAQKAAPGIIQEKW
                     LVLDANTGQNALVQAREFHQHTGLTGLVVTKVDGTAKA"
BASE COUNT          240 a          489 c          530 g          245 t
ORIGIN      
        1 ttttggtttg gacccttttt tccggcagtc cggtccgggt gggtttcacg gaccagcgcc
       61 agggccatcc ttcggggaag atctacaacc tgggcgtgcc cttgtgcccc gaggacctac
      121 aggagcggga caagaactgg aaactggtcg agcgcctctt tcccggcatg ggaaagccgc
      181 ccgcgcttcg gtgcccggcc ctgcccgccg ggacggagcg gaaggtggcg gctttttacc
      241 ggtcggccgg catcggcccc cgggaccggg tgttgggcct gcaccccacc ttgggcaagg
      301 cggacaaccg ctggtcccag aaaaaatacc tggagttgct gcggaggctc tcccggaagg
      361 gagggttcaa attcgtcctg gtccatggct ggggcgaggg ggaagcgctg gcgcggttcc
      421 aagccatggc cgcccgggtc cccggcgtga cggcgcttcc cgaaaccgat cttttcttta
      481 tactgggggc cgcccggagg ttcgccggcc tggtgtgcaa cgacagcggg ttgatgcacg
      541 ccctgtccct ggtgacccgg gtggcggcgg tcttcggccc cagcgaaccc tcgcggtggg
      601 gccccatcgg cccccacgcc caccggatct tccgggcggc ggaccggcgc tgtgattcgg
      661 tgcggcccgc cgacgtggcc cgttacgtcc aaagccgttg gcggggccgc ccatcctgac
      721 attcgccgag gcgcgcgtgg gactgttcga ccaattcaag aagggcctgc aaaagacctc
      781 ccaaagcctg tggggcggcc tgacgggcct gctccagggg gaggccctgt cggaggccga
      841 cctggcccgg ctggaggagg ggttgatcct tgccgacgtc gggccggcca cggcccgggc
      901 cctcgccgcc gaagtgcggg agaagtcccg ccggtcgccc caggcgacgg ccgggctcct
      961 gcgggagtcc ctgttggaac gcctgtcgaa ggtccagtcc cccctggaac tgcccaaacc
     1021 ctcggtgatc ctgttgttgg gcgtcaacgg cgcgggcaag accaccacgg cgggcaaatt
     1081 ggccctgcgt ttcgcccaag aaggccggcg ggtcctgctg gccgccggcg acacctttcg
     1141 ggccgcggcg gaagaacagc tgacggagtg ggcgggacgt gctaaagtgg acctattctg
     1201 cgggaagcac ggcgccgcgc cctccgcagt cgtgttcgat gccgtccagg ccgggatctc
     1261 ccggggggcg gacctggtcc tggtggatac ggccggccga ctgcataacc gccccaacct
     1321 catggaagag cttgaaaaga tcgtgcgggt ggcccaaaaa gccgcgcccg gaataatcca
     1381 agaaaaatgg ctggttttgg acgccaatac ggggcaaaat gcccttgtcc aggcccggga
     1441 attccaccaa cacacgggtt tgaccggttt ggtggtgacc aaggtggacg gcacggccaa
     1501 ggcc
//
LOCUS       DASTJJ010000059         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1476965, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000059 DASTJJ010000000
VERSION     DASTJJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476965"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VFR02_00700"
     CDS             complement(<1..1505)
                     /locus_tag="VFR02_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9869003.1"
                     /translation="MISDQQARDRVVNDHQTLFAVDAGAGTGKTTLLISRLMALLLEK
                     KVALSRVAAITFTEKAAAELSERLRAKLEAALLERPDQKALLLKALEDMERAPISTIH
                     SFCAGLLREYPVEAGVDPQFTLLDEVQSRALEAQAWEHWLKQSLSRPVEPLFHFQRLG
                     GTFAQVEELKQFLKRNLTLLEPEPPKPLPSAEPFRRQLRAFVEMTKKEAAQCAKPEDG
                     LYKVLEGFWAQCASIERVPAAGLAFELAGFQVPKVGNKGAQGVWGKERLAALREAFLR
                     LAEDHQAVLAPFKDAVVLNLVGWIRGYFAEWDRQKAGGGFLDFDDLLLKARDLLLTHL
                     EVREELKDRFDHLFVDEFQDTDPLQVEIVFFLCEAPGKKEKDWRKVELEPGKLFLVGD
                     PKQSIYRFRRADVAIYEATKERILANGGKVEVLTENFRTVGGLVEWVNGLFISQFEGT
                     GIGYHPLTASRKAGATEGTLPILWGLKLPKPPEEKNSKGFWRGWEAEQTAA"
     gene            complement(1502..>1939)
                     /locus_tag="VFR02_00705"
     CDS             complement(1502..>1939)
                     /locus_tag="VFR02_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9869004.1"
                     /translation="MDRVDLSADGKALRVVDYKSGSKEGFKKDSVKAGTKIQMPLYLW
                     ACRTLYPGKSPQEAVYEFITAKGDYGEVGFDAKDPAKIEEPLKALLTTAAEAVEQGLF
                     PAAAKACDRCDYRTLCGPGAERRGERKREDPKVVEYFKLENLP"
BASE COUNT          325 a          633 c          600 g          381 t
ORIGIN      
        1 aatgccgccg tctgttcggc ctcccacccg cgccagaagc ctttggaatt tttttcttcc
       61 ggcggcttgg gcagcttcaa cccccaaaga atggggagcg tgccctcggt cgccccggcc
      121 ttacggctgg ccgtcagggg atggtaccca ataccggtgc cctcgaattg ggaaatgaag
      181 aggccgttga cccactccac cagcccgccc accgtgcgga aattctcggt gaggacctcg
      241 accttgcccc cgttggccag gatgcgttcc ttggtggcct cgtagatggc cacgtccgcc
      301 cggcggaaac ggtagatgct ctgtttgggg tccccgacca ggaaaagttt gccgggctcc
      361 agttccacct tgcgccagtc cttctctttt ttgcccggcg cctcgcacaa aaagaagacg
      421 atctcgacct gcagggggtc ggtgtcctgg aactcgtcca cgaagaggtg gtcgaagcgg
      481 tccttcaatt cttcccgcac ttcgaggtgg gtcaggagca ggtcccgggc cttcaaaagc
      541 aggtcgtcga agtcgaggaa cccgcccccg gccttttgcc ggtcccattc ggcgaaataa
      601 ccccggatcc aacccaccag gttgagcacc accgcgtcct tgaagggggc caggaccgcc
      661 tgatggtcct cggcgagccg caggaacgct tcccgcaggg ccgccaggcg ctccttgccc
      721 caaacaccct gggctccctt gttgcccacc ttggggacct ggaaccccgc cagctcgaaa
      781 gccaggccgg cggcggggac cctttcgatc gaagcgcatt gggcccaaaa accctccaac
      841 accttataaa ggccgtcttc cggcttcgcg cattgggcgg cttctttctt cgtcatttcc
      901 acgaaggccc ggagttggcg ccggaaaggc tccgccgaag gcaggggctt gggaggctcg
      961 ggttccaaaa gggtcaggtt gcgcttgagg aattgcttga gctcctccac ctgggcgaag
     1021 gtgccgccca acctttggaa atggaaaagc ggttccacgg ggcgggacag gctttgtttg
     1081 agccaatgtt cccaggcctg ggcctccagg gcgcgggatt ggacctcatc gagcagggtg
     1141 aattggggat ccacccccgc ttccaccggg tattcgcgca agagccccgc gcagaaggaa
     1201 tggatggtgg agatgggcgc ccgttccatg tcctccagcg ccttcaggag gagcgccttc
     1261 tggtcgggac gctccaggag ggccgcctcg agcttggcgc gcagccgctc ggagagttcg
     1321 gcggccgcct tctcggtgaa ggtgatggcc gcgacccggc tcaaggccac ctttttttca
     1381 aggagcaacg ccatgagccg ggaaatgagc agcgtggtct tgcccgtgcc cgcccccgcg
     1441 tccaccgcga agagcgtctg atggtcgtta acgaccctgt cccgggcttg ctggtcggag
     1501 atcatggtaa gttctccaac ttgaaatact ccacgacctt tggatcttcc cgcttccgct
     1561 cgccccgccg ctcggccccc gggccgcaga gggtgcggta gtcgcagcgg tcgcaggctt
     1621 tggcggccgc ggggaagagg ccctgttcca ccgcctcggc cgcggtggtg aggagcgcct
     1681 tcaagggctc ctcgatcttt gccgggtcct tggcgtcgaa ccccacctcg ccgtagtcgc
     1741 ccttggcggt gatgaattca tagaccgcct cttgggggga cttgccggga taaagcgtgc
     1801 ggcaggccca gaggtaaagg ggcatttgga tcttggtgcc cgccttcacg ctgtccttct
     1861 tgaagccttc cttgctcccg gatttatagt ccacgaccct taaggccttt ccatcggccg
     1921 aaaggtccac ccgatccat
//
LOCUS       DASTJJ010000060         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_131589, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000060 DASTJJ010000000
VERSION     DASTJJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131589"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFR02_00710"
     CDS             <1..601
                     /locus_tag="VFR02_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869005.1"
                     /translation="ALAASPLAAQTPAPAAGPALGETLYQQALDSYLGGDYDQAILLA
                     AKSVEADPQNAKAQSLLGLLTKEKKDIGKYEIWLSTGQGKVPEGPAEDGTAALASSFQ
                     GEIQRVQSHFDHFYRAQTLENGRFQGQIQAVSELLRENSDHQYDELRKSQTDILQQIQ
                     AMNEGRERNLWILYLLCLVSMLFSCSALWAILRRPRGRG"
     gene            722..2038
                     /locus_tag="VFR02_00715"
     CDS             722..2038
                     /locus_tag="VFR02_00715"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9869006.1"
                     /translation="MKRKNRTAAKPRRGRKNLVIIGAQWGDEGKAKMVDVLTEQVDLV
                     ARYQGGSNAGHTVVANGRKFVFHQIPSGILHSGKTCVIGNGVVLDPVALLGEVDSLRR
                     AGVRVADRLVISDRAHLLMPYHRLLDGAAETHKGASKIGTTHRGIGPAYMDKMARVGL
                     RLVDLFDPASFRAKVRTVAREKNFWLKRYFGQKTSDPEAVAAQYLKLAPRLKPLVADT
                     SRYLLNRLGAGKTLLAEGAQGTLLDVDFGTYPYVTSSSASVGGACTGLGIGPQWIDEI
                     YGVAKAYTTRVGEGPFPSEMDPAFAEQIRQRGGEYGATTGRPRRCGWLDLVALRYACR
                     VNGFTGILLTKVDVLSYLKSLKLCVAYRYKGKLLDSFPAGLDQLAECKPVYKEMKGWD
                     GDLSKAASMKDLPREARVYLAEIEKAIQTPITWVSVGQDRKQNFQK"
     gene            2104..2176
                     /locus_tag="VFR02_00720"
     tRNA            2104..2176
                     /locus_tag="VFR02_00720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2137..2139,aa:Lys,seq:ttt)
BASE COUNT          396 a          723 c          724 g          363 t
ORIGIN      
        1 ggccctggcc gcgtcgcccc tggcggcgca gacgcccgcg ccggccgcgg ggcccgcgtt
       61 gggggagacc ctctaccagc aggccctgga cagttacctg ggcggggatt acgaccaggc
      121 catcctcctg gccgccaaat cggtggaggc cgacccgcag aacgccaagg cccaaagcct
      181 gttgggactc ttgaccaagg aaaaaaagga catcgggaag tacgagatct ggctttccac
      241 cgggcagggc aaggtccccg aggggccggc cgaggacggg acggcggctt tggccagcag
      301 cttccagggg gagatccagc gggtgcagag ccacttcgac catttttacc gggcccagac
      361 cctggagaac ggccggttcc agggccagat ccaggcggtc tccgagctgc tgcgcgagaa
      421 ctcggaccac cagtatgacg agttgcgcaa gtcccagacc gatatcctgc aacagatcca
      481 ggccatgaac gagggccggg agaggaacct ctggatcctc tacctgctct gcctggtgag
      541 catgctcttt tcctgctcgg ccctctgggc catcctgcgc cggccccggg gccgcggctg
      601 accctttcgg cccgaagttc cttgtcatcc cccccgtctt cgcttagaat gtggcggttt
      661 gtcccgactc tgcccccggg cggagttttt ttttgccgtc aggaagaggg gagcaggtcc
      721 gatgaaacgt aaaaaccgaa ccgccgccaa gccccgccgg gggcgcaaga acctggtcat
      781 catcggcgcc caatggggcg acgaggggaa ggccaagatg gtggacgtcc tcaccgagca
      841 ggtggacctg gtggcccgct accagggcgg cagcaacgcc ggacacaccg tggtggccaa
      901 cggccgcaaa ttcgtcttcc accagatccc ctcgggcatc ctgcattcgg gcaagacctg
      961 cgtcatcggc aacggcgtgg tcctggaccc ggtggccctg ctgggggaag tggactcgct
     1021 gcgccgcgcc ggcgtgcggg tggccgaccg gctggtgatc agcgaccggg cccatctctt
     1081 gatgccctac caccgcctgc tggacggcgc cgccgagacc cacaagggcg cctccaagat
     1141 cggcaccacc caccgcggca tcgggccggc ctacatggac aagatggccc gggtgggcct
     1201 gcgcctggtg gacctgttcg atccggccag cttccgcgcc aaggtgcgca cggtggcccg
     1261 cgagaagaac ttctggctca agcgctattt cggccagaag accagcgacc cggaggccgt
     1321 ggcggcccaa tacctcaagc tggccccgcg cctgaagccc ctggtggcgg acaccagccg
     1381 gtacctgttg aaccgcctgg gggcgggcaa gaccctgttg gccgaaggcg cccaagggac
     1441 gctgctggac gtggacttcg gcacttatcc ttacgtgacc tcctccagcg ccagcgtggg
     1501 cggcgcctgc acgggcctgg gcatcgggcc ccaatggatc gacgagatct acggggtggc
     1561 caaggcctac accacccgcg tgggggaagg gccctttccc tcggagatgg acccggcctt
     1621 cgccgaacag atccgccagc ggggcgggga atacggcgcc accacgggcc gtccccggcg
     1681 ctgcggttgg ctggacctgg tggccctgcg ctacgcctgc cgggtgaacg gcttcaccgg
     1741 catcctgctc accaaggtgg acgtcttgag ctacctgaag agcctgaagc tctgcgtggc
     1801 ctaccgctac aaggggaagc tcctggacag cttcccggcg ggactggatc aattggccga
     1861 atgcaagccg gtctacaagg aaatgaaggg ctgggatggg gacctgtcca aggccgcttc
     1921 catgaaggac ctgccccggg aggccagggt ctacctggcg gagatcgaaa aggccatcca
     1981 aacccccatc acctgggtct cggtgggaca ggaccgcaaa cagaacttcc aaaagtagcc
     2041 cgcttttcct ttgctttttc catgccgagg cgatagtttt gcctggtcgg aagggttttg
     2101 ggtgagtcgt tagctcagct ggtagagcac cacactttta atgtgggtgt cccgggttcg
     2161 atccccggac gactcaccga aaacctttct ttttttagga ggactc
//
LOCUS       DASTJJ010000061         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1354523, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000061 DASTJJ010000000
VERSION     DASTJJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354523"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFR02_00725"
     CDS             complement(<1..1259)
                     /locus_tag="VFR02_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869007.1"
                     /translation="MSTATQTVSGTHYQELMKEVDAETRKQFEDGLIFFANDADTSGM
                     AEKTRADKLRTAYNLLVEFLVSNRLNDLNLEQRVFLCTGAIDDSVTVMVNNAPKVLAL
                     LPPEFYSLLLKTFSGPATLPYPVFATMDKFTAIAQGELLPLTMGEDRKKDLRNSPQDQ
                     KRAKEEYKRQIAQLKSEMNLSINQMDIHFPKFLNTLNETSIKNAKTALEMMKKVGLGW
                     SKGEAATPQEKAIINAFEGKLGSAGASLGKWVEEMGGYLSKVAEHARLLEEKHQKMNS
                     AINEIAKIDTGAPVVDKTGGPLFDADAIYSIKRDIDNTNSVSVRTADSSPIKVAFSTS
                     RVLVSKQFTEGESFQDRLCSPANVTAALNKVLGIHTNLFAKDFEGKPIIPPILIEPLR
                     NFVDFFQDRFIMGLVSGETARRGAQSV"
BASE COUNT          205 a          356 c          437 g          291 t
ORIGIN      
        1 aagaccgatt gggccccgcg gcgggccgtc tcacccgaca ccaggcccat gatgaagcgg
       61 tcctggaaga agtcgacgaa gttgcgcagg ggctcgatca ggatgggggg gatgatgggc
      121 ttgccctcga aatccttggc gaagaggttg gtgtggatgc cgagcacctt gttcagggcc
      181 gccgtcacgt tggcggggct gcagagccgg tcctggaagc tctcgccctc ggtgaactgc
      241 ttggacacca ggacgcggga ggtggagaag gccaccttga tgggcgagga gtcggccgtg
      301 cgcacgctca cgctgttggt gttgtcgatg tcccgcttga tgctgtagat ggcgtcggcg
      361 tcgaagaggg ggcctcccgt cttgtccacc accggggcgc cggtgtcgat cttggcgatc
      421 tcattgatgg cgctgttcat cttttggtgc ttctcttcca aaagccgcgc gtgttcggcc
      481 accttggaaa gatagccccc catttcctcg acccacttgc ccaaggacgc gcccgcgctt
      541 cccaacttgc cctcgaaggc gttgatgatg gccttttcct gcggcgtggc ggcctcaccc
      601 ttgctccaac ccaggccgac cttcttcatc atctccaggg ccgtcttggc gttcttgatg
      661 gaggtctcgt tgagggtgtt caggaacttg gggaagtgga tgtccatctg gttgatggac
      721 aagttcatct cgctcttcaa ctgggcgatc tggcgcttgt attcttcctt ggcgcgcttt
      781 tggtcctggg gggagttgcg caggtccttc ttgcggtcct cacccatggt gaggggcaaa
      841 agctcgccct gggcgatggc ggtgaacttg tccatggtgg cgaagaccgg atagggcagg
      901 gtggccggac ccgaaaacgt tttcaggagc agcgaataaa attcaggcgg gagcagcgcc
      961 aagaccttgg gggcgttgtt caccatcacg gtcacgctgt cgtcgatggc gcccgtgcag
     1021 aggaagaccc gctgttccag gttcaggtcg ttcaagcggt tggaaacgag gaattccacc
     1081 aacaggttgt aggccgtgcg cagcttgtcc gcgcgggtct tttcggccat gcccgaggtg
     1141 tcggcgtcat tggcgaagaa gatcaggccg tcctcgaact gtttgcgcgt ttcggcgtcg
     1201 acttccttca tcagttcctg gtagtgggtg ccgctgacgg tctgggtggc ggtggacatg
     1261 cggggggcct ccttggattg gtggacgga
//
LOCUS       DASTJJ010000062         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_498600, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000062 DASTJJ010000000
VERSION     DASTJJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498600"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFR02_00730"
     CDS             complement(<1..381)
                     /locus_tag="VFR02_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869008.1"
                     /translation="MPFENRRIIFRWMARPLAGFFLAVGWFFSCPAAWATISNLQIVS
                     VNPAAPVPGAPLTFVGSYCASYNTEPDDFFLAVESASTSIISCPSVNQQFLVDVNGVN
                     RDENDPCGGNASCNQAGFPVTNVNT"
     gene            complement(689..>1120)
                     /locus_tag="VFR02_00735"
     CDS             complement(689..>1120)
                     /locus_tag="VFR02_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869009.1"
                     /translation="DLEAELKGRRPGLTLRTHFLKVRGTAGSSLLEERRMEGPEPDWA
                     ALGASPLLVLDDLWDSGKTLSAARDLVLARTGRRPKSAVLVRKLVPRADGPDFFGLAL
                     DLDPETLAARGLKDLWLFGYGMDLDGRHRELDHIAALEIKA"
BASE COUNT          217 a          351 c          345 g          207 t
ORIGIN      
        1 ggtattgacg ttggtcacgg ggaaccccgc ctggttgcaa ctggcgttgc cgccgcaggg
       61 atcgttctcg tcgcggttga cgccgttgac gtccaccagg aactgctggt tcacgctggg
      121 gcaggagatg atcgaggtgg aggcgctttc caccgccagg aagaagtcgt ccggttcggt
      181 gttgtaactg gcgcaatagg aacccacgaa ggtcaggggg gcgcccggca cgggcgcggc
      241 ggggttgacc gagacgatct gcaggttgga aatggtggcc caggcggccg ggcacgaaaa
      301 aaaccacccg acagccagga agaacccggc caggggacgc gccatccaac ggaagatgat
      361 ccggcgattc tcgaagggca tctcgtctcc aaacgatttt agcgggcgcg gtcaaaaacc
      421 cttgtttggc aaggagaaat cccgcttgaa agccgtgtgg actatttaac ccctcttccc
      481 ggattaagac aatgcgcggg catcttccct gaggaaaagg gtttctctcc cagccctcca
      541 gggtttatcg atttcttctg gacatttcac gtgttaataa aacaggcccc tcccatccgg
      601 gaggggcctg ttattttttt tggaataaac ggcgcacttc ttgtctcaaa gcgacggagg
      661 ctcgtcgacg gccggacccg ctttcccgct aggcttttat ttccaacgcg gcaatgtggt
      721 ccaattcccg atgccggccg tccaggtcca tgccgtatcc gaaaagccaa aggtccttca
      781 atcccctcgc ggccagggtt tccgggtcca ggtcgagcgc caggccgaaa aaatccggac
      841 cgtccgcgcg ggggacgagc ttacggacca ggacggcgct tttgggccgc cggccggtgc
      901 gggccaagac caggtcccgc gccgccgaga gggtcttccc cgaatcccat aggtcatcca
      961 acaccaacag cggcgacgcg cccagggccg cccagtcggg ttccgggcct tccatccgcc
     1021 gctcttccag cagcgaagac ccggccgtcc cccgcacctt caggaaatgg gtccgcaagg
     1081 tcaggcctgg ccggcggccc ttcagttcgg cctccaggtc
//
LOCUS       DASTJJ010000063         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1354763, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000063 DASTJJ010000000
VERSION     DASTJJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354763"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..>1231
                     /locus_tag="VFR02_00740"
     CDS             204..>1231
                     /locus_tag="VFR02_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9869010.1"
                     /translation="MSSSDKSSPIKLHQLGWILVFAVVYADIGTSIFYVPGILYGGIG
                     YLATLAQVITLGVFISIALKYVEICRRCPDGGGVVSVNREAFSSLTFLPLLGGALITV
                     DYFLTSAISGVSGLFYLASLYPPAKIWVVPGSLALFTVLMVLNLIGIRESASLTSTFA
                     ALEMGVMFVLLACSGWWLFAHQGWTPMLSQVIHPGVQLTLPVLLTGYASTWLAYSGLE
                     SAAQLSGAMREPVGKTAWKAMWLVILVVGLLSPTITASALYIVPDAVKRNDPESLLSS
                     LGLVTGGPILEWAVVLAATMLLLMACNTAMVGNYHVNVRLSDLGFMPSFLRRRHARLG
                     TPYWSIIIS"
BASE COUNT          159 a          429 c          362 g          281 t
ORIGIN      
        1 cccgtcattt tgttttcacg ttctttcttg cgccccaaag cctgatttca ggcccttttc
       61 cgggcgcgcc gtccgccgcg gattgaaagt gggggccgtt ccagtatcat gttcgccatt
      121 gtgcccgtgg ggccctcctg aaagagggcg gtttttggga ttcccccggg gatccgattc
      181 ctccagccac aaggttcttt accatgtcca gcagtgacaa gtcctcgccc atcaaactgc
      241 accagttggg ttggatcttg gtcttcgcgg tggtctacgc ggacatcggt acctccatct
      301 tctacgtgcc gggcatcctc tacggcggca tcggctacct ggccaccctg gcccaggtca
      361 tcaccctggg cgtcttcatc tccatcgcgc tcaaatacgt cgagatctgc cgccgttgcc
      421 cggacggcgg cggggtggtg tcggtcaacc gggaagcctt ctccagcctg accttcctgc
      481 ccctcttggg cggggccctc atcacggtgg attatttcct cacctcggcc atctcgggcg
      541 tgtcgggcct cttttacctg gcctccctct acccgccggc caagatctgg gtggtgccgg
      601 ggtcgttggc gctcttcacc gtgctcatgg tcctcaacct catcggcatc cgggaatcgg
      661 cctccctcac ctccaccttc gcggccctgg aaatgggggt catgttcgtg ctcctggcct
      721 gttcgggctg gtggctgttc gcccaccagg gttggacccc catgttgtcc caggtgatcc
      781 atccgggggt gcagctgacc ctgcccgtgc tcttgaccgg ctacgccagc acctggctgg
      841 cctattcggg actggagtcc gccgcccagc tgtcgggcgc catgcgggag cccgtgggca
      901 agaccgcctg gaaggccatg tggctggtca ttttggtggt ggggctcctg tcgcccacca
      961 tcacggcctc ggcgctctac atcgtgccgg acgccgtcaa acgcaacgac cccgagtccc
     1021 tgctgtcctc cctgggactg gtgaccgggg ggcccatcct ggaatgggcc gtggtgctgg
     1081 cggccaccat gctgttgttg atggcctgca acaccgccat ggtgggcaac taccacgtca
     1141 acgtgcggtt gtccgacctg ggcttcatgc cctccttcct gcgccgccgc cacgcccggc
     1201 tgggcacgcc ctactggtcc atcatcatct c
//
LOCUS       DASTJJ010000064         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1232102, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000064 DASTJJ010000000
VERSION     DASTJJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232102"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFR02_00745"
     CDS             complement(<1..1247)
                     /locus_tag="VFR02_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869011.1"
                     /translation="MSLSFFFPSILRAQSCTPSSATLTVAGDDTTTVWIDGSSIGTFA
                     YDNWDSASALPSMSVPVSLLTSYAGYPQVCFEIETQNTAPNVNYTAWDLDITCVGGNH
                     SELTNSSGGLSLYYTPSGNPSTPPPNDGSGNAWNSTAYTATGTYFTYTPTDVTGITWG
                     KSLYDPTTGLLIPPMANNNSGDNSSSNSTGALFWRQCVTIPTPVPTLGPSNFTVTKSL
                     CNGPAYNSPSSNNLSVTYCLNVCNSGAAATGPVTVTDTFSSGAGMQFYGGAAANTDQN
                     TLSGGGCPSVDQGTDPTWSCGDPTQIVFTNGLPGGGSCEGITIALINYYYPNNWCAPV
                     TNIAGVGWSGGTASSSSVSFSIICSPTNTPTPTYTQTRTPTLAVTPTFTPTGTVTPLT
                     ATPTNTPAPVPCGASSGPLLNMQ"
     gene            complement(1237..1404)
                     /locus_tag="VFR02_00750"
     CDS             complement(1237..1404)
                     /locus_tag="VFR02_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869012.1"
                     /translation="MSFSEQPKIDINQIRQFSELGFNENLNQDDQTQLSFYRSGSFPT
                     LVVDFFIGVVA"
     gene            complement(1484..>2745)
                     /locus_tag="VFR02_00755"
     CDS             complement(1484..>2745)
                     /locus_tag="VFR02_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869013.1"
                     /translation="AQGADMITYTLTYSNLGSTTISNAAVTDTLPSGMTYVNGSASNG
                     GLFNAGQVTWALPALLPGASLTLTYQAQVGLQVHTGQVLVNHACLSYGGQVNCASSSV
                     TILGDYVVRVSVFNASGELVKTLDTFELGQPISSFDLGSDVLRTDGDVLQIYADGMSL
                     DAWNGTNAAGQKVSNGTYFVEIDSTDPFGVTTTVTQQATVLLGSNTLDFVVYNSAGEA
                     VLSLDAAQIEALIGGPLTSADFDLGKATFSPLVITPSYADPTGRDTSVLITLGSGRSF
                     RWSGTNDGGKILSPGQYLLSFKGQMSGKPSQNVTTQVTLLGCAGSPIQKVLLKPNPIY
                     LDQTQTAYFEVTVNAPRADNVRVKIYTLAGELTGQVLVNDPGNLSQVTWHLDGVNLAS
                     GMYFADIEVRQGDQLLDRQVLKLAILR"
BASE COUNT          510 a          774 c          926 g          535 t
ORIGIN      
        1 atctgcatgt tgagcagggg tcccgacgag gccccgcagg ggaccggcgc gggggtgttg
       61 gtgggcgtgg ccgtcagggg cgtcacggtc cccgtggggg tgaaggtggg cgtgaccgcc
      121 agcgtggggg tgcgggtctg ggtgtaggtc ggggtgggcg tattggtggg cgaacagatg
      181 atgctgaagg aaacgctgct ggaactggcg gtcccaccgc tccatcccac gccggcgatg
      241 ttggtcaccg gggcgcacca gttgttgggg taatagtaat tgatcagggc gatggtgatc
      301 ccctcgcagg aacccccgcc cggcagcccg ttggtgaaca cgatctgggt ggggtcgccg
      361 cagctccagg tggggtcggt gccctggtcc acgctggggc aaccgccccc cgaaagggtg
      421 ttctggtcgg tgttcgcggc cgcgcccccg tagaactgca tgccggcccc gctgctgaag
      481 gtgtccgtca ccgtgaccgg ccccgtggcg gcggccccgc tgttgcagac gttcaggcaa
      541 taggtgaccg agaggttgtt ggagctgggg ctgttgtagg cgggcccgtt gcagagggac
      601 ttggtgacgg tgaaattgct gggccccagg gtgggaacgg gcgtggggat ggtgacgcac
      661 tgccgccaga aaagcgcgcc cgtgctgttg gacgagctgt tgtcgccgct gttgttgttg
      721 gccatcggcg ggatcaacaa cccggtggtg gggtcgtaaa ggctcttgcc ccaggtgatc
      781 cccgtcacgt ccgtcggggt ataggtgaaa taggtgccgg tggccgtata ggcggtgctg
      841 ttccaagcgt tcccgctgcc gtcgttgggc ggcggggtgg aaggattgcc gctgggcgtg
      901 tagtagaggg acaacccccc gctgctgttg gtcaactccg aatggttccc gcccacgcag
      961 gtgatgtcca ggtcccaggc cgtgtagttg acgttggggg cggtgttctg ggtctcgatc
     1021 tcaaaacaga cctggggata gccggcgtag gaggtcagca gggaaacggg cacgctcatc
     1081 gagggcagtg cactggcgct gtcccaattg tcataagcga aggtcccgat ggagcttccg
     1141 tcgatccaga ccgtggtggt gtcgtcgccg gccaccgtca gggtggcgga agaaggggtg
     1201 caactttggg cccggaggat ggacggaaag aaaaaactaa gcgacaaccc caataaaaaa
     1261 atccacaacc aacgtcggga aagatcccga acgataaaag gagagctggg tttggtcatc
     1321 ttggttgagg ttttcattaa aaccaagttc cgaaaattgc ctaatttggt tgatatcgat
     1381 ttttggttgc tcgctaaagc tcatgacttt tcctttaaat cttaccttat gtttatcatt
     1441 aaagcattca ttaaaccggg taaacggttc ccgaataggc tttttagcgc aaaatggcca
     1501 acttcagcac ctgacggtcc agaagttgat cgccctgcct cacctcaata tcggcgaaat
     1561 acatgccgct ggccaggttc accccgtcca agtgccaagt cacttgcgac aaattgcccg
     1621 gatcattgac cagcacttgg ccggtcaatt cgccggccag ggtatagatc ttcacccgca
     1681 cgttgtcggc acggggggcg ttgaccgtga cctcgaaata agcggtctgg gtctggtcca
     1741 ggtagatcgg gttcggtttc agcaggacct tttggatcgg cgacccggcg cagcccaaca
     1801 aggtgacctg cgtggtcacg ttctgcgagg gcttgccgga catctggccc ttgaaggaca
     1861 gcaggtattg ccccggggaa aggatcttgc cgccgtcgtt ggtcccgctc cagcggaagc
     1921 tgcggcccga acccagcgtg atgagcaccg aggtgtcacg gcccgtggga tcggcgtagg
     1981 aaggcgtgat gaccagcggc gagaaggtgg ctttacccag gtcgaagtcc gccgaggtca
     2041 agggccctcc gatcaaggcc tcgatctgcg ccgcgtccag gctgaggacc gcctccccgg
     2101 ccgaattgta gaccacgaag tccagggtgt tgctgcccag caacaccgtg gcctgctggg
     2161 tcaccgtggt ggtcacgccg aagggatccg tgctgtcgat ctcgacgaaa taggtgccgt
     2221 tggaaacctt ctggcccgcc gcgttggtgc cgttccaggc atccagcgac atcccgtcgg
     2281 cgtagatctg cagcacatcc ccgtccgtgc gcaacacgtc gctgcccagg tcgaagctgg
     2341 agatgggctg gcccaactca aaggtatcca aggtcttcac caattccccg gaagcgttga
     2401 acacggagac ccgcaccacg taatcgccca agatggtcac tgaactgctg gcgcagttca
     2461 cttgcccgcc ataggacagg caggcgtggt tgaccaacac ctggccggtg tggacctgca
     2521 ggcccacctg cgcctggtag gtcaaggtca ggctggcgcc gggcagcagc gccggcaggg
     2581 cccacgtgac ctggcccgcg ttgaacaagc ccccgttcga ggccgaaccg ttcacgtagg
     2641 tcatccccga gggcagcgta tcggtgacgg cggcattgga gatggtcgtg ctccccaggt
     2701 tcgagtaggt caacgtatag gtgatcatat ccgccccctg ggcgg
//
LOCUS       DASTJJ010000065         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_253854, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000065 DASTJJ010000000
VERSION     DASTJJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253854"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..>1425)
                     /locus_tag="VFR02_00760"
     CDS             complement(71..>1425)
                     /locus_tag="VFR02_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9869014.1"
                     /translation="PIGLWGLVRGLGPWLGLVLCYSLIKYLVPVVHPQQFDQSLNEMD
                     VILFGRGNGLFDRTLLGHPHLTDLFCLLYLGLFPWLVGLLVYHSWLRRALYQRFMLGL
                     ILVYIGGFMGYVLYPAIGPRYAYPQDWTWLSGGDIFQWSNVLIAGLGSKFDVFPSLHG
                     ALSAYLLFWQLGHDRRGLVWGLPLTVGIWLSTLVLGFHYFPDLISGGLLAAASSWAAP
                     RVEVLVGAYRRSLEPPRVWLLSLTEGHGDAYGKLAGRLSQIIPLGAEVSPGFITGNVS
                     RARGEAAVRQALADLGPGPFWVRPSDGSGSKKSSLLALKPQSTEQALRQVFDPRAKRF
                     FIVQKAMKVSAVGLCRTFPARGWKLTDVEIRMTALPKGNGLVLRLTPDKTLFKGWLDT
                     PWNYFPKSFPLRGWELFEVVQLTRRLAARWGQLTEVEWILAEGRVYVLDGRAVKAVEA
                     "
BASE COUNT          259 a          485 c          506 g          175 t
ORIGIN      
        1 ggttcgttgg cggccccggc gggccgggcc gccagggcca ggcccaacaa cgccgcccag
       61 cgcaggcgcc tcacgcttcc acggccttca cggcccggcc gtccagcacg tagacgcgcc
      121 cttccgccag gatccattcc acctcggtga gctggcccca ccgggcggcc agccgccggg
      181 tgagctggac cacctcgaaa agctcccaac cccgcaaggg gaaggacttg gggaaatagt
      241 tccagggcgt gtccagccag cccttgaaga gggtcttgtc gggggtcagg cgcaggacca
      301 gcccgttgcc cttgggcagg gcggtcatgc ggatctccac gtcggtcagt ttccagcccc
      361 gggccgggaa ggtgcggcac aggcccacgg cggacacctt catggccttc tgcacgatga
      421 agaagcgctt ggcccggggg tcgaagacct gccgcagggc ttgttcggtg gactggggct
      481 tcagggccag gagcgagctt ttcttcgacc ccgacccgtc cgagggcctg acccagaagg
      541 ggccggggcc caggtccgcc agggcctgac gcacggcggc ctcaccccgg gcgcgggaca
      601 cgttgcccgt gatgaagccc ggcgacacct cggcgcccag ggggatgatc tggctcaggc
      661 gccccgccag cttgccgtag gcgtcgccgt gtccctcggt gagcgacaag agccagaccc
      721 gggggggctc caggctgcgc cggtaggcgc ccaccagcac ctccacccgg ggcgcggccc
      781 aactgctggc cgccgccaag agcccgccgc tgatgaggtc ggggaaatag tggaacccca
      841 gcaccagggt ggaaagccag atgcccacgg tcaggggcag gccccagacc aggccccggc
      901 ggtcgtggcc gagctgccag aagagcaggt aggcggagag cgccccatgc aggctgggga
      961 agacgtcgaa cttggacccc aaccccgcga tgagcacgtt ggaccattgg aagatgtccc
     1021 cgcccgagag ccaggtccag tcctgcgggt aggcgtagcg ggggccgatg gcgggataaa
     1081 gcacgtagcc catgaagccg ccgatgtaga ccaggatgag ccccagcatg aagcgctggt
     1141 acagggcccg cctcagccag ctgtggtaca ccaacagccc caccagccag gggaagagcc
     1201 ccaggtagag caggcagaag aggtcggtca ggtgcgggtg gcccaggagg gtacggtcga
     1261 acaggccgtt gccccggccg aacaggatca catccatctc attgaggctc tggtcgaact
     1321 gctggggatg caccacgggc accaggtact tgatgaggct gtagcagagc accaggccca
     1381 gccaggggcc caggccccgc accaggcccc agaggccgat gggcg
//
LOCUS       DASTJJ010000066         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_988675, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000066 DASTJJ010000000
VERSION     DASTJJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988675"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1014)
                     /locus_tag="VFR02_00765"
     CDS             complement(169..1014)
                     /locus_tag="VFR02_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869015.1"
                     /translation="MSRQFKPEVEKRMNSSPAMNFRVVAAALMLGLGWTSPLFADIDE
                     SAPDDFIHQRTYFGIMGAYGSIDKGKDFNGTVLLTGSVGSSELEADGIPAFDPGYGFS
                     VVLGHRDGAYAGELGFTQTYHTASFTGGTNVTGQNTALYHVLSFDFKRYFLEKLPVQP
                     FVALGLNYTWIDVEDASALVQAGNSTPLAEGSLSIDGLGLDLGAGLEVYMGDGFSLVG
                     GAYERFTGFSGVTGIERASLTPATTNSSSFSLNSTGINFVAGCTVALGAFPVVPEIHR
                     TTRSA"
     gene            complement(1033..1779)
                     /locus_tag="VFR02_00770"
     CDS             complement(1033..1779)
                     /locus_tag="VFR02_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869016.1"
                     /translation="MKSNPRTGSLFAALFTGLLLLTPARLLADEPTQFLYGRTYFGLL
                     GTSASMDGTGFGTATLLASQPYEATLVPSISQNFGWGAFLGRREGAFAAEVSFWQSVH
                     QASWDGSYPVSWSGATPYGQTLKGSATLNMVNVDFKMYFFTEQSLQPFVGLGVDVPWL
                     ETQDTSANGASVGNAVFEGVGFDLGLGAEYYFDTTFSLVAGFYEKWTGFGQYKGVNNV
                     DEQIQNASGHPSSFTANGLDFYIGTTVGFI"
     gene            complement(1885..2967)
                     /gene="ald"
                     /locus_tag="VFR02_00775"
     CDS             complement(1885..2967)
                     /gene="ald"
                     /locus_tag="VFR02_00775"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9869017.1"
                     /translation="MIIGIPREIKDHEYRVGAVPAGVEELAHAGHTLLVERGAGAGTG
                     IPDAEFRAAGARLVRRADLFGRADMILKVKEPQPAEIRLLRRGQVVFGYFHFAASRAL
                     TRAVQRTKCVALAYETLRLPTGEHPLLTPMSEVAGKMAVQEGAKYLEKSMGGKGILLG
                     GVAGVRPAQVAILGGGVVGLNAAKAAAGLGASVTVLDVDPVRLRRLEEVLPKNVSTLV
                     SNRRVLREETARADLLIGAVYVEGAKAPALVTEAMVQAMRPGSVIVDVAIDQGGCVAT
                     SRPTTHSKPVFLKHGVVHYCVTNIPGAVGITSTYALTNATLPYALKIARDGYRRAVET
                     NPDIRHALNLDHGALVHPALQALFGP"
     gene            3094..3393
                     /locus_tag="VFR02_00780"
     CDS             3094..3393
                     /locus_tag="VFR02_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HET9869018.1"
                     /translation="MAMMEISVVPLGTGKTSVSPYIAAIFKHLRKQKKVRYELNGMGT
                     ILTGKAEDLFRLAAQLHRLPLSRGAKRVYTVIKLDDRKDKAQLPIEKVDSVLRKL"
BASE COUNT          717 a         1175 c         1197 g          608 t
ORIGIN      
        1 tttcggtgcg ggtgtccgtg ggggtaaagg aggggttgcc ggccacgatg gtccgcaagg
       61 ccgtggggaa atccccgggg gcggcggggg tctcgacctg gcaggccaga cccaggaaaa
      121 ggggcaggag gatcacggcg gccgataagg aataaagggc gaaacgtttc acgcggacct
      181 cgttgttcga tggatctcag gaacgaccgg aaaagctccc aaagcgaccg tgcaacccgc
      241 cacgaagttg atgcccgtgg agttcaggct gaagctggat gaattcgtgg tggcgggggt
      301 cagggacgcg cgctcgatgc ccgtcacgcc cgaaaaacca gtgaaccgct cataggcgcc
      361 gcccaccagg gaaaagccgt cccccatgta gacctccaag cccgcgccca ggtccaggcc
      421 caaaccgtcg atggacaagc tcccctcggc cagaggcgtg ctgttgcccg cctgcaccag
      481 ggccgaagcg tcctcgacgt cgatccaagt gtagttgagg cccagggcca cgaagggctg
      541 gaccgggagt ttttccagga agtagcgttt gaagtcgaag ctgaggacgt ggtagagcgc
      601 ggtgttctgg ccggtcacgt tggtgccgcc ggtgaaggag gccgtgtggt aggtctgggt
      661 gaaacccaat tcgcccgcgt aggcgccgtc ccggtggccc agcaccaccg agaaaccata
      721 acccgggtcg aaggccggga tgccgtcggc ctccagttcg gaacttccca ccgaaccggt
      781 caagagcacg gtcccgttga aatccttccc tttatcgatg gagccgtagg cccccatgat
      841 gccgaaatag gtgcgctgat ggatgaaatc gtccggggcg ctttcgtcga tgtcggcgaa
      901 gaggggggag gtccacccca ggcccagcat caaggcggcg gccacaactc ggaaattcat
      961 ggcagggctc gaattcatgc gcttctccac ctccggctta aactggcggc tcacgccgca
     1021 tcatagcata aatcaaataa aaccgacggt cgtcccgata taaaaatcca atccgttggc
     1081 ggtgaaactg gaaggatgcc ccgacgcgtt ctggatctgc tcgtccacgt tgttcacgcc
     1141 cttgtactgg ccgaaaccgg tccatttttc gtaaaaaccg gccaccaggg aaaaggtggt
     1201 gtcgaaataa tactcggccc ccagtcccag gtcgaagccg accccctcga agaccgcgtt
     1261 gcccaccgac gcgccgttcg ccgaggtgtc ctgggtctcc agccagggca cgtccacccc
     1321 cagccccacg aagggctgca gggactgctc ggtgaaaaag tacatcttga agtccacgtt
     1381 gaccatgttc agggtggccg accccttgag ggtctggcca taaggcgtgg cgccggacca
     1441 gctgaccgga taactgccgt cccaagaggc ttggtggacg gactgccaga agctcacctc
     1501 ggcggcgaag gcgccttccc ggcggcccaa aaaggccccc catccgaaat tctgggagat
     1561 ggacgggacc agggtggcct cgtagggttg gctggccagg agcgtggccg ttccgaagcc
     1621 ggtgccgtcc atggaggcgg aagtgcccaa gaggccgaaa taggtgcgcc cgtaaaggaa
     1681 ttgggtgggt tcgtccgcca gcaggcgggc cggcgtgagg agcaggagcc cggtgaacag
     1741 cgcggcgaac aggctcccgg tgcgtggatt ggatttcatg gggtcgttgg gtcccttgga
     1801 ggatcaggat gacggctgaa acttcgtgcc atgatactgg attttagacg tcggggaaca
     1861 ggaaagttgg ccgccaaaac ccgctcaagg gccgaaaagg gcctgcaggg ccgggtgcac
     1921 cagcgcgccg tggtcgaggt tgagggcgtg gcggatgtcc gggttggtct ccaccgcccg
     1981 gcggtaccca tcccgggcga tcttgagcgc gtagggcagg gtggcgttgg tgagcgcgta
     2041 ggtcgaggtg atgcccaccg cgcccgggat gttggtcacg caatagtgca ccacaccgtg
     2101 cttgaggaac accggcttgc tgtgggtggt gggccgggaa gtggccacgc agccgccctg
     2161 gtcgatggcc acgtccacga tgaccgaacc cggccgcatg gcctggacca tggcctcggt
     2221 caccagggcg ggggccttgg cgccctccac gtagaccgcg ccgatcagga ggtccgcccg
     2281 ggccgtctcc tcgcgcaaca cccggcggtt ggacaccagg gtggacacgt tcttgggcag
     2341 cacctcctcc aggcgccgca ggcgcaccgg gtccacgtcc agcaccgtca cggacgcgcc
     2401 caggccggcg gcggccttgg cggcgttcag gcccaccacc ccgcctccca ggatggccac
     2461 ctgggccggc cgcacgcccg ccaccccgcc caacaggatg cccttgccgc ccatggactt
     2521 ttcgaggtac ttggcgcctt cctgcaccgc catcttgccc gccacctcgc tcatgggcgt
     2581 gagcagggga tgctcgcccg tgggcagccg cagggtctca taggccaggg ccacgcactt
     2641 ggtgcgttgg acggcccggg tcagcgcccg ggaggccgcg aaatggaaat agccgaagac
     2701 cacctggccc cggcgcagga gccggatctc ggcgggctgg ggttccttga ccttcaggat
     2761 catgtcggcc cggccgaaaa ggtccgcccg gcggaccaac cgggcccccg cggcgcggaa
     2821 ctcggcgtcc gggatgcccg tgccggcgcc cgcgccccgt tccaccagca gggtgtggcc
     2881 cgcgtgggcc agctcctcca cccccgcggg cacggcgccc acgcggtatt catggtcctt
     2941 gatctccctg gggatgccga tgatcacggg cgctcctttc gatccttccc gcccatttta
     3001 cgcccgcttc ggccccaccg ccttctatta aaagccccct gaattggcta tacttggcgt
     3061 ccataaaacg accggttcgg gaaggaggcc gccatggcca tgatggagat cagcgtggtc
     3121 cccctgggga cgggcaagac ctcggtcagc ccctatatcg cggccatctt caagcacctg
     3181 cgcaagcaga agaaggtgcg ttacgagttg aacggcatgg gcaccatcct gaccggcaag
     3241 gccgaggacc tcttccggct cgccgcccaa ctgcaccgcc tgcccctgtc ccggggcgcc
     3301 aagcgggtct acacggtcat caagctggac gaccgcaagg acaaggccca actgcccatc
     3361 gaaaaagtgg actcggtgct cagaaaactc tgacccaatt gggccaaaaa acgtaaaatc
     3421 caaaagccct aaaacgagtc tattttcacc accaaggcac caagacacca agaaaacatt
     3481 ttgattttga aatcgaaaac ttggccgttg ccttccttgg tgtcttggtg ccttggtggt
     3541 aaaagtggtt ttaagtagcg cttagattct caaataagga accccatgct cgaccccaaa
     3601 tacgtgcgtg aacacgaagc cgaggtgcgc caggccctgg cggactgccg gtccaaggtg
     3661 gacctgtccc tgtacctgga cctggagcgc cgccggc
//
LOCUS       DASTJJ010000067         5477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_866899, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000067 DASTJJ010000000
VERSION     DASTJJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866899"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFR02_00785"
     CDS             <1..914
                     /locus_tag="VFR02_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9869019.1"
                     /translation="LGYHRYWLAEHHNFPALASSAPEIMIGQVAAATKTLRVGAGGVM
                     LPNHAPLKVAENFKLLEALFPGRIDLGLGRAPGTDGLTAMALRGSREAVTEDRFPGML
                     GDLLCFLGGAFPDRHPWRKVEAIPAGGGMPDLWMLGSSGSTASLAAQLGLGYAFAHHI
                     HADPAVEACRIYREQYQGTALAPEPRVMLGVLAVAAGTDEEAAELARPVELVRLRLRQ
                     GRKAVYPSPEEARAFIPTAEEAAVLEENRRRMFVGSVERVKEGLLDLAARCGADELMV
                     LTITHDHRDRVRSYEGLARAFGLQPRA"
     gene            958..3372
                     /gene="hrpB"
                     /locus_tag="VFR02_00790"
     CDS             958..3372
                     /gene="hrpB"
                     /locus_tag="VFR02_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET9869020.1"
                     /translation="MELPIDALIPDLLKGLESKNAAVVTAEPGAGKTTRVPPALLGRP
                     FLKGKEVWVLQPRRLAAKMAALRVAEEMGTPVGGAVGYQFRFENKTGPSTRLKFMTDG
                     MLLPLAQSDPTLSRVGAVILDEFHERSLALELGLGWLKRLQSTSRPDLRLIVMSATLD
                     AQALSDYLGGAPVFKSQGRVFPVEVEYLPQPAERDLEAKVRGALRHLSAKAAAGTTLV
                     FLPGMGEIRRCQGALKGLPEVHALYGDLPVEEQQAVLKPSAGRKVILSTNVAETSLTV
                     PGVVAVVDSGLTRQSRVSGWSGLPGLTTVPASQASAAQRAGRAGRLQEGWCVRLYTKF
                     DFDHRAAFDLPELLRSDLSKSLLDLKKLGVGELEAFPWFLKPSPASLESALDLLRRLG
                     ALDQAEDLTDLGRRMASFPLAPRLSRFLLYMKETSPAQLRQACRLAALISEERASSED
                     LLEELRRYQPGYESKRLEDQLAGLVQAGPASGKGDAAEEALSRGLLAAFPDRVGKLRS
                     GEADRHQLGKKELVLSSGGSAQVPDNALTRAHDYFTVVEAQETQQGGKTLVKVRSMAP
                     AEADWLLDLFPEGLKEEQNLRWDAKAQRVEASQRLTYGQLVLDEKALKPSDFGLQARE
                     LLVKEALAAGPQAYADPGALAEFLDRVEFASRHTDKLAPLGPDKIEETLLEISQGCRS
                     LADLREAGLIPALKAKLPPQGQALLEQLAPASLTLGGRRAAIHYEAGKPPWVESRIQD
                     FFGMKQGPTVAGGRVPLVLHLMTPGHKPVQITSDLAGFWERHYPQARKELSRRYPRHK
                     WPESPI"
     gene            complement(3549..4097)
                     /locus_tag="VFR02_00795"
     CDS             complement(3549..4097)
                     /locus_tag="VFR02_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9869021.1"
                     /translation="MKSITALSAALAGLFLATASFAMELTSKDAQNGGTFADAQVFNG
                     MGYKGGNVSPEVSWTKGPEGTLSYVLTMYDPDAPTGSGWWHWVVFNIPASKTELPKGF
                     GSKSYKATKPIVQSRTDFGKGGYGGPAPPPGPAHHYIITVYALKVKSLPLKATDSAAM
                     VGFYANANKLDSAAITVMFGHP"
     gene            complement(4218..4727)
                     /locus_tag="VFR02_00800"
     CDS             complement(4218..4727)
                     /locus_tag="VFR02_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HET9869022.1"
                     /translation="MRGLPLTWIWAGLALFGLLFFLGVYGQLPPVVATHFDAVGNPNG
                     YQTKSAFIAVFLPVMLLPNGLFALFYFLADRVPAERMNLPHKAEWLSTPQNRALLLGK
                     LRGSLVETALFLNLALLFAVQAIYQQNVPGAAFHMPFLAGLVLILGGALVLAVRSLFI
                     FRRPPQDPQ"
     gene            complement(4731..5423)
                     /locus_tag="VFR02_00805"
     CDS             complement(4731..5423)
                     /locus_tag="VFR02_00805"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HET9869023.1"
                     /translation="MSTPLWNKRLKCPFCKFEFETTRLRSSAVRIKETESDFGHLYEG
                     ECAYFYSVTACPQCNFAALNKDFESVRPEYEPKIMEASQKIRATGRKRADIFGLGSSN
                     PETAVRRHELAIAFARIRVYQDLKNLAVLYLHLVWLFRLMGDRDRERAAMAEAAKAYE
                     EYHQKGSDMPEHLGEPGVLYLIGELNRRQGLFKEARRYYERALASKEIKSFPRIAELI
                     RDMMLVAREEMG"
BASE COUNT          933 a         1843 c         1816 g          885 t
ORIGIN      
        1 gcctgggcta ccaccgctat tggttggccg agcaccacaa tttccccgcc ctggccagtt
       61 cggcccccga gatcatgatc ggccaggtgg ccgccgcgac aaagacactg cgggtgggcg
      121 ccgggggggt gatgctgccc aaccacgcgc ccctgaaagt ggccgagaac ttcaagctgc
      181 tggaagcgct cttcccaggg cgcatcgacc tggggctggg ccgggcgccc gggaccgacg
      241 gccttaccgc catggccttg cggggttccc gggaagctgt caccgaggac cgtttccccg
      301 ggatgttggg cgacctgctc tgtttcttgg gcggcgcctt cccggaccgg catccctggc
      361 gcaaggtcga ggcgatcccg gcgggcggcg ggatgccgga cctttggatg ctgggctcca
      421 gcggctccac cgcgtcgctg gcggcccaat tgggcctggg ttacgccttc gcccaccaca
      481 tccacgccga cccggccgtg gaagcctgcc ggatctaccg ggaacaatac caaggcacgg
      541 ccctggcgcc cgaaccccgg gtgatgctgg gcgtgctggc ggtggcggcg ggaacggatg
      601 aggaggccgc cgaactggcc cgcccggtgg aactggtgcg gctgcgcctg cggcaaggcc
      661 gcaaggccgt ctatcccagc cccgaggagg cccgcgcctt catccccacc gccgaggaag
      721 cggccgtcct tgaggagaac cgccggcgca tgttcgtcgg gtccgtggaa cgggtgaagg
      781 aagggttgct cgacctcgcc gcccgttgcg gggccgatga gctgatggtg ctcaccatca
      841 cccacgacca ccgggaccgg gtccggtcct acgaagggct ggcccgcgcc ttcggcctcc
      901 agccccgcgc ctaaaagcgg tccctccccc gcgggaaata tgccaaaatg gccgtccatg
      961 gaacttccca ttgacgccct catccccgac ctgctcaaag gcttggaatc gaagaacgcc
     1021 gccgtggtga ccgccgaacc cggcgcgggc aagaccaccc gcgtgccccc ggccctgttg
     1081 ggacgcccct tcctgaaagg gaaggaagtt tgggtgctgc agccccgccg cctggccgcc
     1141 aaaatggcgg ccctgcgggt ggccgaggaa atggggacgc cggtgggcgg cgccgtgggc
     1201 taccagttcc gcttcgagaa caagaccggg ccctcgaccc ggctcaaatt catgaccgac
     1261 ggcatgctgc tgcccctggc ccaaagcgac cccacccttt cgcgcgtggg cgcggtcatc
     1321 ttggacgagt tccacgagcg aagcctggct ttggaactgg gcctgggctg gctcaagcgc
     1381 ctccagtcca cctcccggcc cgacctgcgc ctcatcgtca tgagcgccac cctggacgcc
     1441 caggccctct cggattacct gggcggcgcc cccgtcttca agagccaggg ccgggtcttc
     1501 ccggtcgagg tggagtacct cccccagccc gccgaacggg acctggaagc caaggtccgg
     1561 ggcgccctgc ggcacctttc ggccaaggcg gccgcgggca ccacgctggt ctttttgccc
     1621 ggcatgggcg agatccgccg ctgccagggg gccttgaaag gcctgccgga ggtgcatgcc
     1681 ctctacggcg acctgccggt cgaggaacag caggcggtct taaaaccctc ggccgggcgc
     1741 aaggtcatcc tctccaccaa cgtggccgaa acttccctca ccgtgcccgg ggtcgtggcc
     1801 gtggtggatt cgggcctcac ccgccaatcc cgcgtctcgg gctggtcggg cctgcccgga
     1861 ttgaccacgg tgcccgccag ccaggcctcc gccgcccaaa gggcgggccg cgccgggcgc
     1921 ctgcaggaag gctggtgcgt ccggctctac accaagttcg actttgacca ccgcgcggcc
     1981 ttcgacctgc cggagctttt gcgctccgac ctgtccaaaa gcctgctcga cctcaagaag
     2041 ctcggcgtgg gggagctgga agccttcccc tggttcctca aaccttcccc cgcctccctg
     2101 gaaagcgccc tggaccttct gcgccgcctg ggcgccctgg accaggcgga ggacctcacg
     2161 gacctgggcc gccggatggc gtccttcccc ctggcgccgc gcctgtcccg gttcctcctg
     2221 tatatgaagg agacctcgcc cgcccaactg cgccaggcct gccgcctggc cgccctcatc
     2281 agcgaggaac gcgcttcctc cgaggacctt ttggaggaac tgcgccgcta ccagccgggc
     2341 tacgagtcca aacgcctgga agaccagctg gcgggcctgg tgcaggcggg ccccgcttcc
     2401 ggaaaaggtg acgcggccga ggaagccctg tcgcgcggcc tgctggccgc ctttcccgac
     2461 cgggtgggca agctgcgctc cggggaagcc gaccggcacc agttgggcaa gaaagaactg
     2521 gtgctgtcct cgggcggcag tgcccaggtg cccgacaacg ccctcacccg ggcccatgat
     2581 tatttcaccg tggtggaggc ccaggagacc cagcagggcg gcaagaccct ggtgaaggtg
     2641 aggtccatgg ccccggcgga agccgactgg ctgttggacc ttttccccga ggggttgaag
     2701 gaggaacaaa acctgcggtg ggacgccaag gcccaacggg tggaggcctc ccagcggctc
     2761 acctacggcc aactggtgtt ggacgagaaa gccctgaagc cctcggactt cggcctccag
     2821 gcccgggaac tgctggtgaa ggaagccctg gccgccggcc cccaggccta cgcggacccc
     2881 ggggccttgg ccgaattcct cgaccgggtc gagttcgcct cccggcacac ggacaagctc
     2941 gcgcccctgg ggcccgacaa gatcgaggaa accctgctgg agataagcca gggctgccgc
     3001 agcctggcgg acctgcggga agccggcctg atcccggcgc tcaaggccaa gctgccgccc
     3061 cagggccagg ccctcttgga gcaattggcc cccgcttccc tcaccctggg cggccgccgc
     3121 gcggccatcc attacgaggc gggcaaaccg ccctgggtgg aatcgcgcat ccaggatttc
     3181 ttcggcatga aacagggccc cacggtcgcg ggcgggcggg tgcccctggt gttgcacctc
     3241 atgaccccgg gccacaagcc cgtccagatc accagcgacc tggccggctt ttgggagcgg
     3301 cattaccccc aagcccgcaa ggagctttcg agaaggtatc cgaggcacaa gtggcctgag
     3361 agtcctattt aacgcgatag cgtcaaaggc ttgccccggc ttacaaacaa aacgagtcaa
     3421 aaggtagccc cgggcggcgt gaggtgagcc ccgctcatct ctgaccggga cctgaacgcc
     3481 ttccgttgtc cgccctccaa caaaaaaggc cgggagggtt tcccctcccg gccttttgac
     3541 gtcggtcctt agggatggcc gaacatcacc gtgatggccg ccgaatccag cttgttggcg
     3601 ttggcgtaaa aacccaccat ggccgccgag tcggtcgcct tcaggggcag ggacttcacc
     3661 ttcaaggcgt agaccgtaat gatgtaatga tgcgccgggc ccggcggggg cgcggggccg
     3721 ccgtagccgc ccttgccgaa gtccgtgcgg gactgcacga tgggcttggt ggccttatag
     3781 ctcttggagc cgaagccctt gggcagttcg gtcttggaag ccgggatgtt gaagaccacc
     3841 cagtgccacc agccgctgcc ggtgggcgcg tccgggtcgt acatggtcag cacgtagctc
     3901 agggtgcctt ccgggccctt ggtccaactc acctcgggcg agacgttgcc gcccttgtag
     3961 cccatgccgt tgaagacctg cgcgtcggcg aaggtgccgc cgttctgcgc gtccttgctc
     4021 gtcaactcca tcgcgaaaga agccgtggcc aggaacaacc ccgccagggc cgccgataat
     4081 gcggtgatgc tcttcatcaa gacgcctccc cgaaaatgat ctggtcaagc gggtccattg
     4141 tagggggatg gttcggatga aagaaagccc cggcgtggcg ccggggcccg ctcaaacacg
     4201 ggggttaaag gaccgaccta ttggggatct tggggcgggc ggcggaaaat aaagagggag
     4261 cggaccgcca acaccagggc cccgcccagg atcaggacca accccgccag gaagggcatg
     4321 tggaaggccg cccccggcac gttctgttgg tagatggcct ggacggcgaa gagcagggcc
     4381 aggttgagga agagcgcggt ctccaccaac gaaccccgca atttccccag gagcagggcg
     4441 cggttctggg gtgtggaaag ccattcggcc ttgtggggca ggttcatgcg ttcggcaggg
     4501 acccggtccg ccaggaaata aaaaagggcg aagagcccgt tgggcaacaa catgaccggc
     4561 aggaagaccg cgatgaaggc gcttttggtc tggtacccat tggggttccc caccgcgtca
     4621 aaatgcgtgg ccaccaccgg cggcaattgc ccatagacgc ccaggaagaa cagcaacccg
     4681 aacaaggcca accccgccca gatccaggta agtggaaggc cccgcatggg tcagcccatc
     4741 tcttcccggg ccaccagcat catgtcccgg atgagttcgg cgatgcgggg gaagctcttg
     4801 atctccttgc tggcgagcgc ccgttcgtaa tagcgccggg cttccttgaa caagccctgc
     4861 cgccggttca gttccccgat gaggtaaagc accccgggct cgcccaggtg ctcgggcatg
     4921 tcggagccct tttggtggta ttcctcgtag gccttggcgg cctcggccat ggcggccctt
     4981 tcccggtccc ggtcgcccat gaggcggaag agccagacca ggtgcaggta gagcacggcc
     5041 aggttcttca ggtcctggta cacgcggatg cgggcgaagg cgatggccaa ctcgtggcgc
     5101 cgaacggcgg tctcagggtt gctggagccc agcccaaaaa tgtccgcgcg tttgcggccc
     5161 gtggcgcgga tcttctggct ggcttccatg atcttgggtt cgtattcggg gcgcacgctc
     5221 tcgaaatcct tgttcagggc ggcgaagttg cactgggggc aggccgtgac cgagtagaaa
     5281 taggcgcatt ccccctcgta gaggtggccg aagtcgctct cggtctcctt gatcctgacg
     5341 gcggaggacc ttaaacgggt ggtctcgaac tcgaatttac agaaggggca tttcaggcgc
     5401 ttgttccaca ggggggtgga catgggtttc ctatgacggg gacagcctag ggctgttcca
     5461 ggtatttttt caggaaa
//
LOCUS       DASTJJ010000068         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_744096, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000068 DASTJJ010000000
VERSION     DASTJJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744096"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFR02_00810"
     CDS             complement(<1..244)
                     /locus_tag="VFR02_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869024.1"
                     /translation="MKGVGGFFIKIAAGSLLAWVGVGAACLLGQSLAHLTWGSPLAVA
                     FAAGFVLYFPVHFLLYRPVLSHVLAHELTHALAAMAL"
     gene            complement(267..1151)
                     /gene="rsmI"
                     /locus_tag="VFR02_00815"
     CDS             complement(267..1151)
                     /gene="rsmI"
                     /locus_tag="VFR02_00815"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9869025.1"
                     /translation="MPEKDAGKNAVRPRTLYLVATPIGNLEDMTFRALQVLRQVDLIA
                     AEDTRHSLKLLTHFGIQAKKLISCFDHNEVKRVPEVLETLAGGGSAALITDAGMPAIS
                     DPGYLVVQAVAAAGFQVSPLPGASSVLAALAASGLPATPFTFLGFLPPKAGDRQKALA
                     RLKSEERTLVFFESPHRLRQSLKDMLNAFGDRKCVVARELTKLHEEVLRGRLEEMGRR
                     FQDREVLGEFVVVVEGGERVDRSAPEKWHGASIEDQLRQFLREGLTKTEAVKAVCKLR
                     DLPREVVYRIATGLKPEA"
     gene            complement(1129..1746)
                     /locus_tag="VFR02_00820"
     CDS             complement(1129..1746)
                     /locus_tag="VFR02_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9869026.1"
                     /translation="MAGALYSLDVSVFRWINEGWSCRALDGFFGFITDIRHFYVVLAL
                     LALFLIFRGGVKGRWMVGALVLTVILTDQTSAHLVKQLVERARPCNALPGVLTPVGGS
                     SAYSFPSSHATNMGGSMLLLALAYPAWAWFFGSFALAVGLSRIYLGLHYPSDVLGGYL
                     LGMAIAGGVWWALRRFVPVFGMKKAAKAPGRPAQRGKARARKRRR"
     gene            complement(1750..2847)
                     /gene="dusB"
                     /locus_tag="VFR02_00825"
     CDS             complement(1750..2847)
                     /gene="dusB"
                     /locus_tag="VFR02_00825"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9869027.1"
                     /translation="MSVLPPENLNAAPAAEPAKPLEGKPLGGEARQLKVGNLTLNGRV
                     FLAPMAGYTDTAFRRLARRYGAAMVITEMVSSRALLEGSEKTEELMAFTEPERPVGVQ
                     LFGGDPGIMGEAAQQVAEKAHPDVIDINFGCPVGKILKCDSGAAVLKEPQRAGWIVKA
                     MVEATGGKVPITVKTRAGYDAMDNAVFELLDAVTEAGASALAVHARTRNQMFEGKANW
                     EVIAKLKQKARIPLIGNGDVKSAADAWRLFQETGCDGIMIGRASMGSPWIFEEINHYL
                     ETGEPMPPMTLKARFGIALEQLKCSLEVKGPRLGLLEMRKHLTHYFKGYEGARDMRQQ
                     LLTSNDPDWVVKALSEIHESLPEDAHVVQVA"
     gene            complement(3001..>3426)
                     /locus_tag="VFR02_00830"
     CDS             complement(3001..>3426)
                     /locus_tag="VFR02_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9869028.1"
                     /translation="LSRMKDGSRDFSFSGLKTAVHLELQKNPVRNEGDLARLAAKFQR
                     TVMEELRWRVRECLEELRPKSLVMGGGVACNQALRQGLEAECRAVGALFRVPPPKYCS
                     DNAAMIAGLGSHHLSRGRRAPLSATAQASPAGPQPFLIP"
BASE COUNT          583 a         1186 c         1125 g          532 t
ORIGIN      
        1 ccaaggccat ggccgccagg gcgtgggtga gctcatgggc cagcacgtgg ctcaggacgg
       61 gtctgtagag caggaaatgg acggggaaat aaaggacgaa cccggccgcg aaggccaccg
      121 ccaggggcga accccaggtg aggtgcgcca ggctttgtcc cagcaggcaa gcggccccca
      181 cccccaccca cgccagcagg gaccccgcgg cgatcttgat gaaaaagccc cccactccct
      241 tcactcggcc cccttggaac gcgtcctcaa gcttccggct tcaacccggt ggcgatccgg
      301 tagaccacct cccggggcag gtcccgcagc ttgcacacgg ccttgaccgc ctcggtcttg
      361 gtcaacccct cccgcaggaa ctggcgcagt tggtcctcga tggaggcgcc gtgccacttc
      421 tcgggcgccg accggtccac ccgctcgccc ccctccacca ccaccacgaa ctcgcccaag
      481 acctcccggt cctggaaacg cctgcccatc tcctccaggc ggccccgcag gacctcctcg
      541 tgcagtttgg tcagttcccg ggccaccacg catttccggt cgccgaaggc gttcagcatg
      601 tccttcaggc tctggcgcaa ccggtggggc gactcgaaaa agaccagggt gcgctcctcg
      661 gacttgagcc gggccagcgc cttctgccgg tcgcccgcct tgggcggcag gaaccccaag
      721 aaggtgaagg gcgtggccgg caaccccgag gccgccagcg ccgccaggac ggaggaagcc
      781 ccgggcaggg gcgacacctg gaaccccgcc gccgccaccg cctgcaccac caggtagccg
      841 ggatccgaga tggcgggcat gcccgcgtcg gtgatgagcg cggcgctccc gccccccgcc
      901 agggtttcca ggacctcggg cacccgcttg acctcgttgt ggtcgaaaca agagatgagc
      961 ttcttggctt ggatgccgaa atgggtcaaa agtttcaggc tgtggcgggt gtcttccgcc
     1021 gcgatgaggt ccacctgccg cagcacctgc agggcccgga aggtcatgtc ctccaggttg
     1081 ccgatgggag tggccaccag gtagagggtg cggggccgga cggcgttctt accggcgtct
     1141 ttttcgggca cgggccttcc ccctttgggc cggccggccg ggcgccttgg ccgctttttt
     1201 catcccgaag acgggcacga aacggcgcag ggcccaccac acgcccccgg cgatggccat
     1261 gcccaacaga tacccgccca gcacgtccga ggggtaatgc agccccaagt aaatacgcga
     1321 caggcccacc gccagggcga aggacccgaa gaaccaggcc caagccggat aagccagggc
     1381 caagagcagc atggacccgc ccatgttggt ggcgtggctg gaagggaagg aataggccga
     1441 actgccgccc acgggcgtga gcacgccggg gagcgcgttg cagggacgcg cgcgttccac
     1501 cagttgcttc accaaatggg ccgaggtctg gtcggtgagg atcacggtca gcacgagcgc
     1561 ccccaccatc cagcgcccct tcacgcctcc ccggaagatg aggaagagcg ccagcagggc
     1621 cagcaccacg tagaaatggc ggatgtcggt gatgaagccg aagaagccgt ccagcgcccg
     1681 gcaggaccaa ccttcgttga tccagcggaa gaccgagacg tccagggaat agagggcgcc
     1741 ggccaagcct caggccacct ggacgacgtg ggcgtcctcg ggaagcgact catggatctc
     1801 cgaaagggcc ttcaccaccc aatcggggtc gttggaggtc aagagctgct ggcgcatgtc
     1861 ccgggcgccc tcatagccct tgaaatagtg ggtgaggtgc ttgcgcatct ccaaaaggcc
     1921 cagccgcggg cccttcacct ccagcgagca cttgagctgt tccagggcga tgccgaaacg
     1981 ggccttgagg gtcatggggg gcatgggctc acccgtctcc aggtaatggt tgatctcctc
     2041 gaagatccag ggcgagccca tgctggcccg gccgatcatg atgccgtcgc acccggtctc
     2101 ctggaacagg cgccaggcgt ccgcggccga cttcacgtcg ccgttgccga tgagggggat
     2161 gcgggccttt tgcttcaact tggcgatgac ctcccagttg gccttgccct cgaacatctg
     2221 gtttcgggtg cgggcgtgca cggccagggc ggaggcgccc gcctcggtca cggcgtccaa
     2281 gagctcgaac accgcgttgt ccatggcgtc atagcccgcg cgggtcttga ccgtgatggg
     2341 caccttgccg cccgtggcct ccaccatggc cttgacgatc cacccggcgc gctggggctc
     2401 cttcaggacg gccgcgcccg agtcgcactt gaggatcttg cccaccgggc agccgaagtt
     2461 gatgtcgatg acgtccgggt gcgccttctc ggccacctgc tgggccgcct cgcccatgat
     2521 gcccgggtcg cccccgaaca actgcacgcc caccggccgc tcgggctcag tgaaggccat
     2581 gagttcctcg gtcttctcgc tgccctcgag cagcgcccgg gaactgacca tctcggtgat
     2641 gaccatggcg gcgccgtaac ggcgggccag gcggcggaag gccgtgtcgg tgtagccggc
     2701 catgggggcc aaaaagaccc ggccgttcaa ggtgaggttg cccaccttca attggcgcgc
     2761 ttccccgccc aggggttttc cttccagggg cttggccggc tcggccgccg gggcggcgtt
     2821 caggttttca gggggcagca cgctcatcgt tccaccttct gcgatctcaa gtcggaccgt
     2881 cttgaaccga agcacaacat ggatccacgg agttttaagg ggccggacat ctttctaggg
     2941 acccgaagtc tccgttcggg cccacccaca acggcatttt agcacgcccg ggggcggggt
     3001 ctagggtatc aggaagggct gggggccggc gggcgaggcc tgggccgtgg ccgacagggg
     3061 cgcccggcgt ccccgggaca ggtggtggga gccgagcccg gcgatcatgg ccgcgttgtc
     3121 cgagcagtac ttgggcggcg gcacgcggaa aagggcgccc acggcccggc attcggcctc
     3181 caggccctgc cgcagggcct ggttgcaagc cacccccccg cccatcacca ggctcttggg
     3241 cctcaattct tccaagcatt cccgcacccg ccagcgcagc tcctccatca cggtgcgctg
     3301 gaacttggcc gccaaccggg ccaagtcccc ctcgttacgg accggatttt tctggagctc
     3361 caggtggacg gccgtcttga ggccgctgaa ggaaaaatcc cggctgccgt ccttcatgcg
     3421 tgacag
//
LOCUS       DASTJJ010000069         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_378088, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000069 DASTJJ010000000
VERSION     DASTJJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378088"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFR02_00835"
     CDS             <1..243
                     /locus_tag="VFR02_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869029.1"
                     /translation="SSEIELQLEEVTKAKAQIVIYAPSKVISKMVNFWNGDPSLSSEQ
                     GKKAFVAMLYAMRADTFQHDHLVSEDDLKLLLLGKE"
     gene            complement(293..>1392)
                     /locus_tag="VFR02_00840"
     CDS             complement(293..>1392)
                     /locus_tag="VFR02_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HET9869030.1"
                     /translation="ASLGCATGRVSLSLSSYFIGMGLGQILYGPFLDHFGRKRPLYVG
                     ILLYVASSVACVASPSLGALIAFRFLQALGGCAAGVAATAMVRDFFHVKESAKVFSLL
                     MLILSVSPLLAPTVGGFVAQTLGWRWIFLILGAISLAILAVVFFFLPEGHKPDPKASL
                     SLKKSLSNFAMILRHPQFYAYTLAGSLSFAGLFAYIAGSPVIFLEVYHVGTKAYGAIF
                     ALLAAGFIGGSQLNLWLLKRFSSEEVLRFAIAFQALTGCGVALFAWMGWLNLSGTIAF
                     VFLVLACGGLVNPNAGALSLAPFGDHAGSAAALMGFIQITAGSLASALVGFLNAGTIF
                     PTAAVMAGSSLAGLAAIVLVRPPKGRRRGRP"
BASE COUNT          339 a          416 c          442 g          195 t
ORIGIN      
        1 tcatcagaaa tcgaattaca acttgaggaa gtgaccaaag caaaagcgca aatcgtcatc
       61 tatgcgcctt ccaaagtcat atccaagatg gtcaacttct ggaacgggga tccaagccta
      121 tcatcggagc aagggaagaa agcatttgtt gcgatgttgt acgcaatgcg ggcagataca
      181 tttcaacacg atcatttagt cagtgaagac gatttgaaat tacttcttct agggaaagaa
      241 taagtttcgg gtccagggac ctctcgttca aatttgattc ttcttactca cttcacggac
      301 gtccacgacg cctccctttc gggggacgga ccaggacgat ggcggccagc cccgccaggg
      361 aggacccggc catcaccgcc gccgtgggga agatggtccc ggcgttgagg aaacccacca
      421 gggccgaggc caaggaaccg gcggtgatct ggatgaaccc catcagggcc gccgcgctgc
      481 ccgcgtggtc cccgaagggc gcgagcgaca gggcccccgc attggggttc accaacccgc
      541 cgcaggcgag caccaggaaa acgaaggcga tggtgccgga caggttcaac caccccatcc
      601 aggcgaaaag ggccacaccg caccccgtca gggcctggaa ggcgatggca aaccgcagca
      661 cctcttcgct gctgaagcgt ttcaggagcc agaggttcaa ctggctcccc ccgatgaagc
      721 ccgccgccag cagggcgaag atggccccat aggccttggt ccccacgtgg tagacctcca
      781 aaaagatgac cggcgacccc gcgatgtagg cgaagagccc cgcgaaggaa agcgaccccg
      841 ccagggtata ggcatagaac tgggggtgcc tgaggatcat ggcgaagttg gaaaggcttt
      901 tcttgaggct gagggaagcc ttggggtcgg gcttgtgccc ctcgggcagg aagaaaaaga
      961 ccaccgccag gatggccagg gagatcgcgc ccaggatgag gaagatccag cgccagccca
     1021 gcgtctgggc cacgaacccg cccacggtgg gcgccaacaa gggcgacacg ctcaggatga
     1081 gcatgagcaa cgagaagacc ttggcgctct ccttcacgtg gaagaaatcc cgcaccatgg
     1141 cggtggccgc cacgcccgcc gcgcagccgc cgagcgcctg caggaaacgg aaggcgatga
     1201 gcgcccccag ggaaggggag gccacgcagg ccaccgagga ggccacgtag agcaggatgc
     1261 cgacatagag gggccgcttg cggccgaagt ggtccaggaa ggggccgtag aggatctggc
     1321 ccaaccccat gccgatgaaa tagctggaaa gggacaagga cacccgcccc gtggcgcagc
     1381 ccaacgaagc gg
//
LOCUS       DASTJJ010000070         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_500225, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000070 DASTJJ010000000
VERSION     DASTJJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500225"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFR02_00845"
     CDS             complement(<1..889)
                     /locus_tag="VFR02_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869031.1"
                     /translation="MRPARRRIIGFLTLAAALLGPSGARAVQMDPLPFENFSQDGFAF
                     SVYRYEKPSFYLLSSGLPDLFFAGIKELSSPTPGRPNDPLTFSLRGVEYGFQASAWPF
                     EDLQLRLSLPFEATALEDLSGVTQSRQNVGDLEAGATWLLTGKRSGGDFLGLDGWFRF
                     ATGTNPFGEAAYPILATGKGTPEGSLGAVLRQQVGRFSLFESVHYVHPLPLALGDNPY
                     LGPGTFQWPDELQALGRIEFLAFERGERLVTLFYEYRFRKVGTMSFQGEPLPYDDGHF
                     ADQLFYSGGGLTVRVDSELT"
     gene            complement(902..1486)
                     /locus_tag="VFR02_00850"
     CDS             complement(902..1486)
                     /locus_tag="VFR02_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869032.1"
                     /translation="MKVRAFFAGVLVCLAGPSGLWAAPSFWAPKGTLTPTPVAAPPVW
                     VPDEPAQYLTVTPTPVTAPFVGYATPTPTPEPGVSHKDPATAAIFSVVVPGAGQVYDG
                     DPLKGLAFAAVFGVSLWQTIDSLQLEPDPNNPGDVIAKDEERGSLFGLITLAAYGFGI
                     EDALDGAVDYNRRHYVSLALGMRPLASASLTYHF"
     gene            complement(1562..>2164)
                     /locus_tag="VFR02_00855"
     CDS             complement(1562..>2164)
                     /locus_tag="VFR02_00855"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9869033.1"
                     /translation="YLDLVEKIGSFHSQLAESYIQDIRVEYLGEVSKYDPKPLTLSLV
                     KGILQKGMSSSVNYVNALFMAKEKGYSLSETVNAEARDYSSLIRVTVKNQKNTHVITG
                     TVFGKHEARVVGLDGNTTDFSPVGNMIWMAHQDKPGIVGRLGTILGNNSVNIAGLYVG
                     RQQVGGNAIAMVNVDSEVPEQVMQELRQIPGIQELKFIRF"
BASE COUNT          380 a          683 c          750 g          351 t
ORIGIN      
        1 ccgtcagttc cgaatccacc cggacggtca atccgccgcc ggagtaaaaa agctggtccg
       61 cgaaatgccc gtcatcgtag gggagcggct ccccctggaa ggacatggtg ccgaccttcc
      121 ggaagcggta ctcataaaag agggtgacca accgttcgcc ccgctcgaag gccaggaact
      181 cgatgcggcc cagggcctgc aactcgtccg gccattggaa cgtcccggga cccaggtaag
      241 gattgtcgcc cagcgcgagg ggcaagggat ggacgtagtg tacgctctca aaaagcgaaa
      301 atctaccgac ttgttgccgc aggacggcgc ccagcgagcc ctcgggcgtg cccttgcccg
      361 tggccaggat cgggtaggcc gcctcgccga aagggttggt gcccgtggcg aaccggaacc
      421 aaccgtccag ccccaggaag tccccgccgc tgcgcttccc cgtcaagagc caggtcgcgc
      481 ccgcctccag gtcccccacg ttctggcggc tctgggtcac gcccgacagg tcctccagcg
      541 cggtggcctc gaagggcagg gacagccgca gttgcaggtc ctcgaagggc caggcgctgg
      601 cctggaagcc gtattcgacc ccgcgcaggg agaaggtcaa ggggtcgttg ggccggcccg
      661 gggtggggct ggatagttcc ttgatgccgg cgaagaagag gtcgggcagg cccgagctga
      721 gcaggtaaaa actgggtttt tcgtagcggt agaccgagaa ggcgaagccg tcctgcgaga
      781 agttctcgaa agggaggggg tccatctgga cggcccgggc ccccgaaggg cccaggaggg
      841 ccgccgccag ggtcaggaag ccgatgatcc gtcgccgggc cggtcgcacg gggacgccgg
      901 tttaaaagtg gtaggtcagg gaggcgctgg ccaggggccg catcccgagg gcgagggaca
      961 cgtaatggcg gcggttgtaa tccaccgcgc cgtccagggc gtcctcgatg ccgaagccgt
     1021 aggccgccag ggtgatcagg ccgaagagcg acccgcgctc ctcatccttg gcgatgacgt
     1081 cccccggatt attgggatcc ggttccaact gcaggctgtc gatggtctgc caaagggaaa
     1141 cgccgaaaac ggcggcgaag gccaggccct tcaaggggtc cccgtcgtac acttggcccg
     1201 cgcccgggac caccaccgag aagatcgcgg ccgtggccgg gtccttgtgg gacacgccgg
     1261 gctcgggcgt gggggtgggg gtggcgtagc cgacgaaggg cgcggtgacc ggggtggggg
     1321 tgacggtcag gtactgggcc ggttcatcgg ggacccaaac cgggggcgcc gccaccgggg
     1381 tgggcgtgag ggtccccttg ggggcccaaa aactgggggc cgcccacagg cccgaggggc
     1441 ccgcgaggca gaccaggacc ccggcgaaga aggctcttac tttcatggcg ctaaggtatc
     1501 tcccccccgg gggcctgtca agcgacaggc tcttcccggc ggttcccctc cccgcggagg
     1561 ttcaaaagcg gatgaatttc agctcttgga tgcccgggat ctgccgcaat tcctgcatga
     1621 cctgctcggg cacctcactg tccacgttga ccatggcgat ggcgttcccg cccacctgct
     1681 ggcggcccac atagagcccc gcgatattga ccgaattgtt gcccaggatg gtgcccaacc
     1741 ggcccacgat gcccggcttg tcctggtggg ccatccagat catgttgccc accggcgaga
     1801 agtccgtggt gttgccgtcc aggcccacca cgcgggcctc gtgcttgccg aagaccgtgc
     1861 ccgtgatgac gtgggtgttc ttctggttct tgaccgtcac ccggatgagc gacgaatagt
     1921 cccgggcctc cgcgttgacg gtctcggaaa ggctgtagcc cttttccttg gccatgaaga
     1981 gcgcgttcac gtagttcacg gaggagctca tgcccttctg caggatgccc ttcaccaggc
     2041 tcagggtgag cggcttgggg tcgtacttgg acacctcgcc caggtattcc acccggatgt
     2101 cctggatgta ggactcggcc aattgggaat ggaaggaccc gatcttctcc accaggtcca
     2161 ggta
//
LOCUS       DASTJJ010000071         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_744705, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000071 DASTJJ010000000
VERSION     DASTJJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744705"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFR02_00860"
     CDS             complement(<1..610)
                     /locus_tag="VFR02_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET9869034.1"
                     /translation="MNPPYDAVLLIGFGGPERPEDVLPFLRDVTQGKDIPEERLQKVA
                     KQYEQIGGKSPYNHLTHEQAELLEQLLAARGLPIPVLTGFAHAGPRVADSLGFLARQG
                     KLNVFAIVMAPHRSPASFDKYTALVDRALESLKAQGFPAMRITYAPEWHTRPGFINAM
                     TDHVKEAYAQLTPEEKARHRAELIFTTHSIPKAMAMRSPYSKQ"
     gene            861..>1059
                     /locus_tag="VFR02_00865"
     CDS             861..>1059
                     /locus_tag="VFR02_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869035.1"
                     /translation="MGKTRPREQENLELIDLVRGFAILSVLLFHFVLGHVTAMGGPSL
                     FRWISAHFWINGAYGVQVFFVV"
BASE COUNT          168 a          284 c          381 g          226 t
ORIGIN      
        1 actgtttgga atagggcgaa cgcatggcca tggccttggg gatgctgtgg gtggtgaaga
       61 tgagctcggc gcggtgccgg gccttttcct caggggtcag ctgggcgtag gcctccttca
      121 catggtcggt catggcgttg atgaagccgg gccgggtatg ccattcgggc gcgtaggtga
      181 tgcgcatggc cgggaacccc tgggccttga ggctttccag ggcccggtcc accagggccg
      241 tgtacttatc gaaggaagcg gggctgcggt ggggcgccat gacgatggcg aagacgttca
      301 attttccctg gcgggccaaa aagcccaggc tgtcggcgac gcgggggccc gcgtgggcga
      361 agcccgtgag cacggggatc ggcaggcccc gggccgccag gagctgttcc agcagttcgg
      421 cctgctcatg ggtgaggtgg ttgtagggcg acttgccgcc gatctgctcg tattgcttgg
      481 cgaccttttg gagccgctct tcggggatgt ccttgccttg ggtgacgtcc ctcaaaaagg
      541 gcaggacgtc ttcggggcgc tccgggccgc cgaagccgat gagcaggacc gcgtcgtagg
      601 gtgggttcaa ggtcgggcat cctaaaacag aagggatctc ggcgaggcct agttatagca
      661 aagaaaagag gggatgtctt gggtgggtca aggcgttgcc catgggccga ccgaaaggct
      721 ccaacgctcc ccggtcgatc gtggggtccg gggacggcgg ccgatgccct tccagttcgg
      781 gcgttttcag gcccattcca cgggggcgtg gattaggatg ttgcccatgc cgttcatcct
      841 gatcggataa ggtggcgcct gtggggaaaa cccggccccg agaacaagaa aacctggaac
      901 tcatcgacct ggtccggggt ttcgcgatcc tttcggtcct gctttttcat ttcgtcctgg
      961 gacacgtcac ggcgatggga gggccttccc ttttccgctg gatctccgcg catttttgga
     1021 tcaatggcgc ttatggggtg caggtctttt tcgtggtct
//
LOCUS       DASTJJ010000072         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_867821, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000072 DASTJJ010000000
VERSION     DASTJJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867821"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..211)
                     /locus_tag="VFR02_00870"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="VFR02_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(300..>1498)
                     /gene="ltrA"
                     /locus_tag="VFR02_00875"
     CDS             complement(300..>1498)
                     /gene="ltrA"
                     /locus_tag="VFR02_00875"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET9869036.1"
                     /translation="QAYLKVAANKGAAGVDEVSLAEFKSDLKGNLYKIWNRMSSGSYF
                     PPPVKAVEIPKAHGAGTRILGVPTVGDRIAQTVAAARIEAAVEPKFHPDSYGYRPRKS
                     ALDAVGKCRERCWKCNWVIDLDVQKFFDTVPWDRIIAAVEANIRLPWVILYVKRWLAA
                     PIMMPDGTLAERDRGTPQGSAVSPVLANLFMHYAFDLWLGRKFPGCPFERYADDAVVH
                     CRTKAQAEFVLDRLRKRMEQAGVSLHPEKTRIVYCKDGKRRGSHEHAEFTFLGFTFRA
                     RGLRARNGNVFTGFAPAVSKDAIKKMSAQVKSWRLHALTGHTIGEIARAVNPVIRGWM
                     RYYGAFYKTELHSVLRRINAYLVRWIRKKYRRLRPVKKAHGAWKRITGQYPALFAHWQ
                     WVHNPW"
BASE COUNT          248 a          509 c          437 g          304 t
ORIGIN      
        1 tgtttctgcc cttgtacggg acctttgccc aggtagagcc ttgatatccc ttatcggcca
       61 ggacgatgac gccctgccgt tccagctcgt cgagcacgcc ccagatccat tccgccttct
      121 tgtcgtggac cgagccgggc agcgccccgg agacccacag gacctcgccg tcgggactgg
      181 cgatgacctg caggttcatg ccgtgctttt tgtcgggtgg cctgggggat tctcaccccc
      241 aggctcccgc agatcccggc gtgacagtct cccgtcaccg ggctcttctc atcctctcat
      301 caccacgggt tatggaccca ttgccagtgc gcgaagaggg ccggatactg gccggtgatc
      361 ctcttccacg ccccgtgcgc cttcttgacg ggccgcagcc gccggtactt cttcctgatc
      421 caccgcacca ggtacgcgtt gatgcggcgg aggacggaat gcaactcggt cttgtagaag
      481 gcaccgtaat accgcatcca tccccggatt accgggttga ccgcgcgggc gatctccccg
      541 attgtgtggc cggtgagcgc gtgcagccgc catgacttca cttgcgcgct cattttcttg
      601 atggcgtcct tgctgacggc cggggcgaac ccggtgaaaa cgttcccgtt cctggccctt
      661 agccctctgg cccggaaggt gaacccaagg aaggtgaatt cagcgtgctc atgcgagccg
      721 cgccttttcc cgtccttgca gtaaacgatc cgggtcttct cagggtgcag ggacacgccg
      781 gcctgctcca tcctcttccg cagcctgtcc agcacgaact cggcctgggc cttcgtccgg
      841 cagtggacca ccgcgtcatc ggcgtaacgc tcaaaagggc agccagggaa cttccgtcca
      901 agccacaagt cgaacgcgta atgcatgaac aggttagcga gaacgggact gaccgccgac
      961 ccttgcgggg ttccgcggtc cctttccgcc agcgttccgt cgggcatcat aatgggagcg
     1021 gcaagccacc gcttcacata gaggattacc cagggcaggc ggatgttggc ctcaacggcc
     1081 gcgataatgc ggtcccaggg aacggtgtcg aagaacttct gcacgtccag gtcaatgacc
     1141 cagttgcatt tccagcacct ttcccggcat ttccccacgg cgtccagcgc gctcttccgg
     1201 ggccggtagc cgtacgagtc cgggtgaaac ttcggttcca ccgcagcttc tatccgggcc
     1261 gccgccacgg tctgcgcgat acggtcgccg acagtcggga cgccgaggat cctggttccc
     1321 gcgccatgcg ccttcgggat ttcgaccgcc ttcaccgggg gcgggaagta cgaacccgat
     1381 gacatccggt tccagatctt gtagaggttc cccttcagat cggacttgaa ttccgccagg
     1441 ctgacttcat cgaccccggc ggcgcccttg ttggccgcca ccttcagata tgcctgcc
//
LOCUS       DASTJJ010000073         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_744911, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000073 DASTJJ010000000
VERSION     DASTJJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744911"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFR02_00880"
     CDS             complement(<1..956)
                     /locus_tag="VFR02_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9869037.1"
                     /translation="MKYANSVLDLIGNTPLVKLNKVTFGLQATVLVKLEYMNPGGSAK
                     DRIAVKMLDEAAKEGKIKPGGTVVEPTSGNTGVAMAMVAQQRGYNCVFVVPDKVGEDK
                     RAVLEAYGAEVVVTPTAVDADSPQSYYGVSDRLVRETPGAYKPDQFSNQNGPLSHYET
                     TGPEIWRDTEGKVTHFVAGVGTGGTITGTGRYLREASKDRPEADGGEVRIIGADPEGS
                     VYSGGTGRPYLVEGVGEDMWPASYDPKVPHEIIAVTDNDSFAMTRRLAREEGLLVGGS
                     SGMAVVAALEAAKGLGPEAVVVVLLPDSGRGYLAKIFDDKWM"
BASE COUNT          161 a          366 c          351 g          202 t
ORIGIN      
        1 tgcatccact tgtcgtcaaa gatcttggcc aggtagccgc gcccgctgtc cggcaggagc
       61 acgacgacga cggcctccgg tcccagcccc ttggcggctt ccagtgcggc caccacggcc
      121 atgccggagg atccgcccac cagcaggcct tcttcccggg ccaggcggcg ggtcatggcg
      181 aaggagtcgt tgtcagtgac ggctatgatc tcgtgcggga ccttgggatc gtacgaggcg
      241 ggccacatgt cctcgcccac accctccacc aggtacggcc ggcccgtgcc gccggagtac
      301 acggagcctt ccgggtcggc gccgatgatc cggacttcgc cgccgtcggc ctccgggcgg
      361 tccttggagg cttcgcgcag gtacctgccg gtgccggtga tggtgccgcc ggtgcccacc
      421 ccggcgacaa agtgggtgac cttgccttcg gtgtcgcgcc agatttcggg cccggtggtc
      481 tcgtagtggc tcagcgggcc gttctggttg gaaaactggt ccggcttgta ggcgccggga
      541 gtctcccgca ccagccggtc ggaaacgccg tagtaggact gcgggctgtc ggcgtcgacc
      601 gccgtagggg tcaccacaac ctcggcgccg taggcctcca ggacggcacg cttgtcctcg
      661 cccaccttgt ccgggaccac gaacacgcag ttgtagccgc gctgctgcgc caccatggcc
      721 atggccacgc cggtgttgcc gctggtcggt tccaccacgg tgccgccggg cttgatcttg
      781 ccttccttgg cagcctcgtc cagcatcttg acggcaatgc ggtccttggc ggaaccaccc
      841 gggttcatgt actcaagctt caccaggacg gtggcctgga gcccaaaggt caccttgttc
      901 agcttgacca acggggtgtt gccaataagg tcaaggacgg agtttgcata cttcataagt
      961 ccaaagtatc cccgccgtgt cagtccgacc aaaggaggtt accgtttggg gcttctgcgg
     1021 ccctaggatc ggaagagtgc tcaccgcttt ggatgccctc ccggaccggc cccggcgcgt
//
LOCUS       DASTJJ010000074         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_500596, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000074 DASTJJ010000000
VERSION     DASTJJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500596"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFR02_00885"
     CDS             complement(<1..405)
                     /locus_tag="VFR02_00885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET9869038.1"
                     /translation="MRTNFVDSPVGQLKKHTAPVPYLGGLAVYFAFLLALFGSMVFLN
                     DPADTPKILALVVGGTVMALLGLVDDLFSLSPGVKFLVQFAAAACLFLFGVHIKFILN
                     PWLDGAVTLVWVVGVTNALNLIDIMDGLAGGVA"
     gene            complement(871..>2289)
                     /locus_tag="VFR02_00890"
     CDS             complement(871..>2289)
                     /locus_tag="VFR02_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9869039.1"
                     /translation="KERGELEKALEQYRLVLAGSPKNILALGNLGDALMALERFEEAV
                     KTYKKALKLLPESGERLNLQNDLGVAYFKKGEIESAIAEFKAVLKADPDHVNAIYNLG
                     QVYYHEGLTGRMKKDYEEFVKSSKDAASILFSLSKSMVSVATAAGKGPGQDDPSLVGN
                     SPAMKKVQDLIKRAAASDATVLILGENGTGKELVAKAIHQQSNRYDKPFVPIACSALS
                     ESLLESELFGHEKGSFTGAVGLKVGRFEAADHGTVFLDEIGEISLSTQVKLLRVLQER
                     EFERVGGTESLKVDIRVIAATNRDLKQAIQAERFREDLYYRLNVIVIETPPLRRREGD
                     LPLLIRHFIDRQKAKRSTRFEGVSGEALRLMEQYAWPGNVRELENVVERILTLNDDTL
                     IRPEHLPDEIQGRRGPGIPETARPLTAILPGASVLETVEKEMIVRVLKESDFNKKQAA
                     VRLGISRPTLYQKIRKYGIEVP"
BASE COUNT          403 a          708 c          732 g          446 t
ORIGIN      
        1 ggccacgccc cccgccagcc cgtccatgat gtcgatgagg ttcaaggcgt tggtcacgcc
       61 caccacccag acgagcgtca ccgccccgtc cagccagggg ttgaggatga acttgatgtg
      121 cacgccgaag aggaacaggc aggccgcggc ggcgaattgg accaggaact tgacgcccgg
      181 gctcagggag aaaaggtcgt ccaccaggcc cagcagggcc atcaccgtgc ccccgacgac
      241 cagcgccagg atcttgggcg tgtcggcggg atcgttgagg aagaccatcg atccgaagag
      301 ggccagcagg aaggcgaagt agacggccag cccccccagg tagggcacgg gggcggtgtg
      361 cttcttcaac tgccccacgg ggctgtcgac gaaattggtc ctcaaggcga tccggcggaa
      421 gaggggggtc agtcccaggg aaatgagggc ggcggcggcc aggatgatcc agtcggtgtt
      481 caggggcgtc ctccggggag gtttggcggc aatggctagg ataacatagg aaaagggggg
      541 cttgcccccg gaaggcagga ccccagggcg ttcctcctgg ggccgggccg atccgaggga
      601 aatagcctat tcacgggcaa aaaaaaagcc ccggcgggtc aagaccgggg ctctcaggag
      661 gtgagaatgg tttggttcgt taaaaagcta ctccgctctc acggatgagt tatagataag
      721 caaggcttat gccaaggagg tctttggaaa aggcccttaa aaacggcctc ttttttgact
      781 tgtgtaaaga ccccttcaat ctttgtgtta acttattata cacaattgtt tacacagttc
      841 aatatcttta cacattcacc gtttgtccgt tcaaggcacc tcaatgccgt atttccggat
      901 cttttgatac agcgtgggcc ggctgatgcc caaccgcacc gccgcctgct tcttgttgaa
      961 gtccgattcc ttgagcaccc gcacgatcat ttccttttcc accgtctcca ggacgctcgc
     1021 ccccggcagg atggccgtga gggggcgcgc cgtctcgggg atcccgggcc cgcggcgccc
     1081 ctggatctcg tccggcaggt gttcgggacg gatcagggtg tcgtcgttga gggtgaggat
     1141 gcgttccacg acgttctcca attcccgcac gttgcccggc caggcgtact gttccatcaa
     1201 ccggagggcc tccccggaca ctccctcgaa ccgggtggag cgcttggctt tctgccggtc
     1261 gatgaaatgg cggatgagca gcggcaagtc gccctcgcgc cggcgcaggg ggggcgtctc
     1321 gatgacgatg acgttgagcc ggtaatagag gtcctcgcgg aaccgctcgg cctggatggc
     1381 ctgcttgagg tcccggttgg tggccgcgat gacccggatg tcgaccttga gggactcggt
     1441 gccgcccacc cgctcgaatt ccctctcctg cagcacccgc aacagcttca cctgggtgga
     1501 gagggagatc tccccgatct cgtccaggaa gacggtcccg tggtccgccg cctcgaagcg
     1561 ccccaccttc agcccgaccg cccccgtgaa ggaacccttc tcgtggccga aaagctcgct
     1621 ctcgagcaag gactcgctga gcgccgagca ggcgatgggc acgaagggct tgtcgtagcg
     1681 gttggactgc tggtggatgg ccttggccac cagttccttg cccgtgccgt tctcgcccag
     1741 gatgagcacg gtggcgtcgc tggcggcggc gcgcttgatc aggtcctgga ccttcttcat
     1801 ggccgggctg ttgcccacca gcgaggggtc gtcctgcccc gggcccttgc ccgccgccgt
     1861 ggccaccgac accatggatt tggagagcga aaagaggatg gaagccgcgt ccttggacga
     1921 tttgacgaat tcctcgtagt ccttcttcat gcggccggtc agcccctcgt ggtaatagac
     1981 ctggcccagg ttgtagatgg cgttcacgtg gtccgggtcg gccttcagca cggccttgaa
     2041 ctcggcgatg gcgctctcga tctcgccttt cttgaaatag gccaccccca ggtcgttctg
     2101 caggttgagc cgctcgccgc tttcgggcag gagcttcagg gccttcttgt aggtcttgac
     2161 cgcctcctcg aaacgctcca gggccatgag cgcgtcgccc aggttgccca gggccaggat
     2221 gttcttgggg ctgccggcca gcaccagccg gtactgttcc agggcctttt ccagctcgcc
     2281 ccgttcctt
//
LOCUS       DASTJJ010000075         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1724155, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000075 DASTJJ010000000
VERSION     DASTJJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724155"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFR02_00895"
     CDS             <1..651
                     /locus_tag="VFR02_00895"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9869040.1"
                     /translation="GELEVELTPQGTLAEKLRAGGAGIPAFYTAAGVGTQVSEGGLPR
                     KYDGAGGVAIASQPKEVRTFNGADFVLEESLNPDFALVHAWKGDRHGNLVFHATAMNF
                     NPLCAMAGKITIAEVEELVEPGELDPEHVHVPGIFVQRVVHVPAGTPGSEKRIEKRTV
                     ADPGEAVTVPPPVPSPAPTSQARPGTPGAVAPSRAPEAAPGAPSRPRLIESTTKEA"
     gene            654..>1233
                     /locus_tag="VFR02_00900"
     CDS             654..>1233
                     /locus_tag="VFR02_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HET9869041.1"
                     /translation="MALTRNELAARVARELHNGQYVNLGIGMPTLIPNFIPEGVEVVL
                     HSENGILGTGPYPLDADVDPDLINAGKETVTVNKGAAFFDSALSFGMVRGGHVDVAVL
                     GAMQVAQNGDLANWMIPGKMVKGMGGAMDLVFGAKKLIVMMEHTDRAGNPKILESCTL
                     PLTGKGCVDLIVTDLAVIEVTPDGLVLKELAPG"
BASE COUNT          222 a          417 c          419 g          175 t
ORIGIN      
        1 ggtgagctcg aggtggaact gacgccgcag ggcaccctgg ccgagaagct gcgcgcgggc
       61 ggcgccggga tccctgcgtt ctacacggca gcgggcgtgg gcacccaggt ctccgagggc
      121 ggactgccca ggaagtacga cggcgctggc ggcgttgcga ttgcatccca gcccaaggag
      181 gtgcgcacct tcaacggcgc cgactttgtg ctggaggagt ccctgaaccc cgactttgcg
      241 ctggtccatg cctggaaggg cgaccgccac ggcaacctgg tcttccacgc cacggccatg
      301 aacttcaatc cgctgtgcgc catggccggg aagatcacca tagccgaggt ggaggagctg
      361 gtggagcccg gcgaactcga ccccgagcac gtccacgtcc cgggcatctt cgtccagcgc
      421 gtggtccacg tcccggccgg cacccccgga agcgaaaagc gcattgaaaa gcgcaccgtc
      481 gcggacccgg gcgaggccgt gacggtcccc ccgcccgtcc ccagcccggc cccaacctcg
      541 caagctcggc cggggactcc cggggccgtg gccccatcgc gggcgcccga agccgctccc
      601 ggggccccat cacggccccg cctcattgaa tccaccacga aggaggcctg accatggcac
      661 tgacacgcaa cgaactggcc gccagggtgg cgcgggaact gcacaacggg cagtacgtga
      721 acctgggcat cggcatgccc accctgatcc caaacttcat tccggagggt gtggaggtgg
      781 tgctgcattc ggaaaacggg atcctcggca ccggccccta cccgctggac gcggacgtgg
      841 acccggacct gatcaacgcc ggcaaggaaa cggtcacggt caacaagggc gcggcgttct
      901 ttgattccgc cctgtcgttt ggcatggtgc gcggcgggca cgtggacgtc gccgtcctgg
      961 gtgccatgca ggtggcccaa aacggcgacc tcgccaactg gatgatcccg ggcaagatgg
     1021 tcaagggcat gggcggcgcc atggatctgg tgttcggcgc caagaagttg attgtcatga
     1081 tggagcacac cgacagggcg ggaaacccga agatcctgga aagctgcacc ctgccgctca
     1141 cgggcaaggg ctgcgtggac ctgattgtca ccgacctggc cgtcatcgag gtgaccccgg
     1201 acgggctggt cctgaaggaa ctggcacccg gcg
//
LOCUS       DASTJJ010000076         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1479768, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000076 DASTJJ010000000
VERSION     DASTJJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479768"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFR02_00905"
     CDS             complement(<1..804)
                     /locus_tag="VFR02_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9869042.1"
                     /translation="MKDFLEKSIKKISDASTRAAKYSEKLDHFSHIVTRQTLLDLAGK
                     KLKDRPLLLVSNREPYAHVMRDGAVTAIRNAGGLTIALDSIAQAFHSTWVCQGSTEAD
                     FQVLDDKGLVAVPPAAPTYKLRRLRLTPDEEHGYYEGFSNETLWPLCHVCYVRPKFSP
                     TDWETYDQVNLKFAQAVAESAEPGAVVFLQDYHLSRTAWHLKTLRPDLVTALFWHIPW
                     PNPEIFRILPWKAEILEGLLANDILGFHLKYHADNFMDTVDLELESRIDR"
     gene            1091..1339
                     /gene="rpsP"
                     /locus_tag="VFR02_00910"
     CDS             1091..1339
                     /gene="rpsP"
                     /locus_tag="VFR02_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9869043.1"
                     /translation="MSVKIRLKRMGSKKKPYFRLIVVDSRFPGKGRRVEDVGTYNPRS
                     TDNHVLNLNQEAIAAWVKKGAVPSETVTGILKKAGVKL"
     gene            1362..1595
                     /locus_tag="VFR02_00915"
     CDS             1362..1595
                     /locus_tag="VFR02_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9869044.1"
                     /translation="MMKELIEYVAKSLVDKPGDVALQERLEGGLTVLELRVSKDDYGK
                     VIGKQGRTIKAIRTLIAASCAKNGLKYSLEVVE"
     gene            1588..2142
                     /gene="rimM"
                     /locus_tag="VFR02_00920"
     CDS             1588..2142
                     /gene="rimM"
                     /locus_tag="VFR02_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9869045.1"
                     /translation="MSNPDTPPPQPNEFTVLGTIVRPHGLKGQVKVSLSCSGLERLAG
                     CPDLRLVREGRELKKVSVAKGFLHNDGDAVLLLREVSGVDEAEALRGAQLCVPAGAEA
                     PLPEDSYYLHDLKGLRVETTAGEPLGVIDDFLETPANWVCVAKDGGKETLIPALKSVI
                     RRVDFKGRRMVVELPEEIDEADAD"
     gene            2132..2872
                     /gene="trmD"
                     /locus_tag="VFR02_00925"
     CDS             2132..2872
                     /gene="trmD"
                     /locus_tag="VFR02_00925"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9869046.1"
                     /translation="MRIDILTLFPGYFEGPLTVSMVKRAVDKKALDLRVTYLRDFAEG
                     KRRTADDVPYGGGAGMVLKPEPLFKAIRAIKRGRRGVKTLLMSPQGRRLDHGLVMELL
                     KEKSLLLVCGHYEGLDERVMGLIDGEVSLGDYVLTGGEPAAAVLVDALARFLPGVVGD
                     RESVEKDSFFNGLLDHPHYTRPHVFRGLKVPEVLLSGNHQKVERWRRKESLRNTLVKR
                     PDLLERAALSDEDRELLREIRAEQTPKP"
BASE COUNT          568 a          871 c         1035 g          527 t
ORIGIN      
        1 ccggtcgatg cggctttcca gttccaggtc caccgtgtcc atgaaattat ccgcgtggta
       61 cttgaggtgg aaacccagga tgtcgttggc cagcaggccc tccaggatct cggccttcca
      121 gggcaggatg cggaagatct ccgggttggg ccaggggatg tgccagaaga gggcggtcac
      181 caggtcgggg cgcagggtct tcaggtgcca ggccgtgcgg ctcaagtggt agtcctgcag
      241 gaacaccacg gccccgggct cggccgattc ggcgacggcc tgggcgaact tcaggttgac
      301 ctggtcgtag gtctcccagt cggtggggga gaatttgggg cgcacgtagc agacatggca
      361 caggggccag agggtctcgt tggaaaagcc ctcgtagtag ccgtgttcct cgtcgggggt
      421 cagccgcagg cggcgcaact tataggtggg ggcggcgggg ggcacggcca ccaggccctt
      481 gtcgtccagc acctggaaat cggcttccgt ggagccctgg cagacccagg tggaatggaa
      541 ggcctgggcg atggaatcca gcgcgatggt gagcccgccg gcgttgcgga tggccgtcac
      601 ggcgccgtcg cgcatcacgt gggcataggg ctcccggttg gacaccaaga gcagggggcg
      661 gtccttcagc ttcttgccgg ccaggtccag cagggtctgg cgggtgacga tatgggagaa
      721 atggtcgagt ttttcgctgt atttggccgc ccgggtggag gcgtcggaga tcttctttat
      781 ggatttctca aggaagtcct tcatgcatga tctcccgggg gataaagggt gtggatcatc
      841 caagaccggc tggtttcagc gtttcctggc cggtacaggg aatcgtcaat taaggggttt
      901 aacgcgatct ccatccccac cttaccccct tcgaaagccg aaatgcaacc aacgattggc
      961 cgggaaaggg gttaaatcaa tggaaaaccc ggagggcgcc gaaagggcct atttccttga
     1021 caaaggcccg gggggttttt agaatccatc ctctttacta ttctttcgcc gtgatttccg
     1081 aggaggatcc gtgtccgtca agattcgttt aaagcgcatg ggcagcaaga agaagcccta
     1141 tttccgcctg atcgtcgtcg attcccgttt cccgggcaag ggccgccgcg tggaggatgt
     1201 gggcacctac aacccccgtt ccaccgacaa ccacgtgttg aacctgaacc aagaggccat
     1261 cgccgcctgg gtcaagaagg gcgccgtgcc ctccgagacc gtcacgggca tcctgaaaaa
     1321 agccggcgtc aagctttgat ccgccgatcc gagagaaggc catcatgaaa gaactcattg
     1381 aatacgtcgc caagtcgctg gtggacaagc ccggggacgt cgccctgcag gagcgcctcg
     1441 aaggcggcct gacggtgctg gagcttcggg tgtccaagga cgattacggg aaggtgatcg
     1501 gaaagcaggg ccggaccatc aaggccatcc gcaccctgat cgccgcttcc tgcgccaaga
     1561 acggcctcaa gtactccctc gaagttgttg agtaacccgg acacaccccc accccaaccc
     1621 aacgagttca cggtcctggg caccatcgtg cggccgcacg ggctcaaggg ccaggtcaag
     1681 gtttccctga gctgctcggg gctggaacgc ctggccggct gccccgacct gcggctggtg
     1741 cgcgaggggc gcgagctcaa gaaggtctcg gtggcgaagg gcttcctgca caacgacggc
     1801 gacgccgtcc tgctgctgcg ggaggtctcg ggcgtggacg aggcggaagc cctgcggggc
     1861 gcccaactct gcgtgcccgc cggcgccgag gcgccgctgc ccgaggattc ctactacctg
     1921 cacgacctga agggcctgcg ggtggagacc accgcggggg aacccctggg cgtgatcgac
     1981 gatttcctgg agaccccggc caactgggtc tgcgtggcca aggacggcgg gaaagagacc
     2041 ctgatccccg cgctcaagtc cgtgatccgg cgggtggatt tcaagggccg gcgcatggtg
     2101 gtggaactgc ccgaggagat cgatgaggcc gatgcggatt gacatcctga cgcttttccc
     2161 gggttacttt gagggtcccc tcacggtgag catggtgaag cgggcggtgg acaagaaggc
     2221 cctggacctg cgggtgacct acctgcggga cttcgccgag ggcaagcggc gcacggccga
     2281 cgacgtgccc tacgggggcg gggccggcat ggtgctcaag cccgagcccc tcttcaaggc
     2341 catccgggcg atcaagcggg gccgccgggg ggtcaagacc ctcctgatgt cgccccaggg
     2401 ccggcgcctg gaccacggcc tggtgatgga gttgctgaag gaaaagtccc tgctcctggt
     2461 ctgcggccat tacgagggcc tggacgagcg ggtgatgggc ctcatcgacg gggaagtgag
     2521 cctgggggat tacgtgctga ccggggggga acccgcggcg gcggtcctgg tggacgccct
     2581 ggcccggttc ctcccagggg tggtagggga ccgggaatcg gtggaaaagg acagtttttt
     2641 taatggatta ttggaccatc cgcattatac tcgtccccac gtttttcgag gcctgaaggt
     2701 tcccgaggtt ttgctctcgg gcaaccatca aaaggtcgaa cgctggcgcc gcaaggaatc
     2761 cttgcgcaac acgctggtga agcgccccga ccttttggag cgggcggcgc tgagcgacga
     2821 ggaccgggaa ctgctgcggg agatccgggc ggaacaaacc ccgaagcctt gaaggacccc
     2881 gccggggcgg ggtgacattg aaggagaaaa agatgaacgc cattatcaag aagatcgacg
     2941 acgcccagat catccgcaag gaagccggtt tccgggtggg cgacaccgtg aaggtgtccc
     3001 t
//
LOCUS       DASTJJ010000077         6294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1357245, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000077 DASTJJ010000000
VERSION     DASTJJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357245"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFR02_00930"
     CDS             complement(<1..416)
                     /locus_tag="VFR02_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET9869047.1"
                     /translation="MTAEKAYAELLTRMKELALLGNTAGVLGWDQEVYMPGANAAYRA
                     DQLSLLAGMCHQKFTDPAVGDLIDRALREPGDPRGDQAVNLREWKRSYERSKKLPQSL
                     VEEMARVTSNAQVEWVEARKAKDFKRFQPWLEKIITL"
     gene            complement(549..1523)
                     /locus_tag="VFR02_00935"
     CDS             complement(549..1523)
                     /locus_tag="VFR02_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9869048.1"
                     /translation="MKYRVLGASGPEVSEISLGCWTLGGLNWVNGDPNGWADVDEAEV
                     EKAVHHALDAGVNHFDNADVYGNGKAERLLARALGPRVKKLILATKVGWFPGTAAHAY
                     EPAHIRHQCEQSLINLKRDVIDLYYFHHGNFGEKDLYLDDAVAVMRKLKQEGKIRLIG
                     QSAYSSGDFERLVPKVQPDVLQSWGHIMDDQFIREGSPVRRLLEERRMRFVAFSPLNQ
                     GVLLGKFDPQHPPKFEAGDHRLGDKRFTTESLLQVQPKLEKLKARFGGEVKDMARVAL
                     QYVLSHPVVACVIPGFRNLRQVQLDLEGADKPLSPEDVAFVRGTFNNI"
     gene            1819..2235
                     /locus_tag="VFR02_00940"
     CDS             1819..2235
                     /locus_tag="VFR02_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9869049.1"
                     /translation="MKKYSDFRILIAEDEPGIRRIYEKTFAQDGYEVVMATSGGEVMA
                     ELEEGKFDLLITDLKLEHTSALDVLPYIRKNHPALPIVIVSGHYVQLTNDFKSRGYNV
                     KMFFNKPLGMNVLRDCVRKILGAPEPVAAEAKPSFF"
     gene            complement(2265..3206)
                     /locus_tag="VFR02_00945"
     CDS             complement(2265..3206)
                     /locus_tag="VFR02_00945"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9869050.1"
                     /translation="MTSEIEKAARILQEGGLVAFPTETVYGLGADATNVMAVARVFEA
                     KDRPQFDPLIVHVADLRQAQILALRFPRDAVRLAEKFWPGPLTLVLPKGPLIPDLVTA
                     SLPSVALRVPDHPLALGLIQASGRPIAAPSANPFGAVSPTTAAHVRESLGDKVDLVLD
                     GGPCAVGVESTVVSFTGDRPLLLRPGGLALEEIEALLGPVEVVAASQSLPQAPGQLER
                     HYAPRTPLTLVEAVPSLSKNERAGLLWFGPPQDLPSFKTVVNLSPQGDLREAAANLFS
                     SLRRLDALGLDRLYALQVPGTGLGRAINDRLRRAAHR"
     gene            complement(3333..6155)
                     /gene="ppc"
                     /locus_tag="VFR02_00950"
     CDS             complement(3333..6155)
                     /gene="ppc"
                     /locus_tag="VFR02_00950"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET9869051.1"
                     /translation="MFQEETKQLDLAAMPKADSYALPHLVRVLAGTLGQVIADQEGPD
                     TLARVEKVRVLCKDLRAQPSPAIVEALNQIFSRLSVDKLNQLTKAFTHYFGLINLAEK
                     LDQIHQARDAAQKDPQAKREGSIPAAIQALRKQGIPPKKIQGLLDLARITMVFTAHPT
                     ESKRPTTLEKLHRIAKSARRMVSEDALPEERQDRMQHIVEELVSLWQTDEIRQFKPDV
                     LDEVKGHLYYFEETLFKVVPKLYRELEKSLQEEFPKTAWDIRPFLTFGSWMGGDRDGN
                     PFVTPEVTVETLKVMRRCALHFYSTSMEMLSDRLSSSCRQVPISDRLRQSLELDSLLF
                     PDLADRLNQRTPNEMYRQKCSFIYEKLRRTMAHTADYDGVTPGDDGAPAPGTWYAHAG
                     QLMEDLDVMDESLRSHKGSVLADGFLEDIRTRVRVFGLQLAKLDLRQHSGRHAAALAE
                     IFKYVGLADDYLSLPEEEKIALIVKELESHRPLIPERTPFTPETLETIETFRMANHVL
                     EHLDSQAAESYIISMTKNISDVLGVLLFFREAGLFKPDSYSRLNVVPLLETSQDLQNA
                     GQFLDRLFNIPIYRKHLEFRNQQQEVMLGYSDSNKEGGYLAANWALYRAQVELVRVAD
                     NHQVTLRLFHGRGGSIGRGGGPANKAILAQPPKTIRGSIKITEQGEVISDLYSEPDNA
                     RGHLEQIVNAVLRGSFPPREVVPRRNWELVMDKLAAVSFKAYRGLIYDHPRFLEYFSS
                     ATPINEISQHRIASRPSSRSAKRSIEDLRAIPWVFSWMQSRHTIQGWFGAGTAIEEYL
                     KGDPQGLAVLQEMYGQWPFFQRLLDNTQMILSKADMDIARRYAELVPDAVLGREIFGR
                     IEGEYNRTVSVIRQVTQVGEMLEREPALSQSIKLRNPYIDPLSFIQIELLRRARNHPS
                     EEEKSHLEDAILLTINGIAAGLKNTG"
BASE COUNT         1029 a         1897 c         2173 g         1195 t
ORIGIN      
        1 agggtgatga tcttttccag ccagggttgg aagcgcttga agtccttggc cttgcgggcc
       61 tccacccatt ccacctgggc gttggaagtg acccgggcca tctcctccac cagggactgg
      121 ggcagcttct tggaccgctc gtaggagcgt ttccattccc gcaggttgac ggcctggtcc
      181 ccccggggat cgccgggttc cctcaaagcc cggtcgatca ggtcccccac ggccgggtcg
      241 gtgaactttt ggtggcacat gccggccaag agcgacaact ggtccgcccg gtaggccgcg
      301 ttggcgcccg gcatatagac ctcctggtcc cagcccaaca ccccggcggt gttgcccaac
      361 agggccagtt ccttcatgcg ggtcaaaagt tcggcatagg ctttttcggc ggtcatggcg
      421 tcctcccagg atgaacgatc aaagactttt taccacgaag tacacgaagg acacaaagtt
      481 caaaccggta ttatttttgg tttgaaagcc gtactttgtg tacttggtga acttcgtggt
      541 gaaaggattc agatgttatt aaaggtcccc cggacgaagg cgacgtcctc ggggctcagg
      601 ggtttgtccg cgccctccag gtccagttgg acctgccgca ggttgcggaa tcccgggatc
      661 acgcaggcca ccaccgggtg ggacagcacg tattgcaggg ccacccgggc catgtccttc
      721 acttcgccgc cgaagcgggc cttcagcttt tccagcttgg gctgcacctg aaggagcgac
      781 tcggtggtga agcgcttgtc gcccagccgg tggtcgccgg cctcgaactt gggcgggtgt
      841 tgggggtcga acttgcccag cagcaccccc tggttgagcg gcgagaaggc cacgaatctc
      901 atccggcgct cttccaggag ccggcggacc ggcgagcctt cgcggatgaa ctggtcgtcc
      961 atgatgtggc cccagctctg cagcacgtcg ggttgcacct tgggcaccag ccgctcgaaa
     1021 tcgccgctcg aataggccga ttggccgatg agccggatct tgccttcctg cttcaacttg
     1081 cgcatgacgg ccacggcgtc gtccaggtaa aggtcctttt ccccgaagtt cccatggtgg
     1141 aaataataaa ggtcgatcac gtcgcgtttg aggttgatca gcgactgctc gcactggtgg
     1201 cggatgtggg cgggctcata ggcgtgggcg gcggtgccgg ggaaccagcc caccttggtg
     1261 gccaggatga gtttcttgac cctcgggccc agggcgcggg ccagcaggcg ttcggccttg
     1321 ccgttgccgt agacgtcggc gttgtcgaaa tggttcacgc ccgcgtccag cgcgtggtgg
     1381 acggcctttt ccacttccgc ctcgtccacg tcggcccagc cgttgggatc gccgttcacc
     1441 caattgagcc cgcccagggt ccagcagccc aggctgatct cggagacttc cgggcccgaa
     1501 gcccctaaaa cacggtattt catggcggtt cctttggcca aaagtggctg agggacgttt
     1561 ttaccattaa tggggccgga aaagaacggt tttcttgaac cggttcagcg cggtggggat
     1621 gggcccatcc ggggcggggc tggctcgatc cgcccttttt ttgtccgacg ggtcattgcg
     1681 ctccgcgtca ttccaggcct gttgagttct tttttcccag ggatcgggat ggaaggcgtg
     1741 gtttttcaag gttttggatt ggcacgaggg ttgcaatgac ctataggcag aagcacatca
     1801 aaccagagga gcagcgccat gaagaaatat tccgacttcc gtatcctgat cgccgaggat
     1861 gagccgggca tccgccggat ctacgagaag accttcgccc aggacggtta tgaagtggtg
     1921 atggccacct cgggcggcga ggtcatggcc gagttggaag agggcaagtt cgacctgctc
     1981 atcaccgacc tcaagctgga acacaccagc gccctggatg tgctgcccta catccgcaag
     2041 aaccacccgg ccctgcccat cgtgatcgtg tcgggccact acgtgcagct caccaacgac
     2101 ttcaagagcc ggggttacaa cgtgaagatg ttcttcaaca agcccttggg gatgaacgtg
     2161 ctgcgggatt gcgtgcgcaa gatcctgggc gcgccggagc ccgtggcggc cgaagccaag
     2221 ccctcgttct tttgaggtcc ggagggccgc ccctccgatc ggggtcagcg gtgggcggcc
     2281 ctgcgcaacc ggtcgttgat ggcccggccc aagccggtcc cggggacttg gagggcgtaa
     2341 agccggtcca ggcccaaggc gtccagccgg cgcagggacg agaaaaggtt ggcggcggcc
     2401 tcccggaggt cgccttgggg ggacaaattc accaccgtct tgaaggacgg aagatcttgg
     2461 ggcgggccga accataagag gcccgccctt tcgtttttcg aaagggaagg gaccgcctcc
     2521 accagcgtga gcggggtacg gggcgcgtaa tggcgttcca actgccccgg ggcctggggc
     2581 agggactggg aagcggccac cacctccacc ggcccgagga gggcctcgat ctcctccagg
     2641 gccaggccgc ccggacgcag caggaggggc ctgtcccccg tgaaggacac cacggtcgat
     2701 tccaccccca cggcgcaggg cccgccatcc aggaccaggt ccactttgtc ccccagggac
     2761 tcgcgcacgt gcgcggcggt ggtggggctg acggcgccga agggattggc gctgggcgcg
     2821 gcgatgggcc gcccggacgc ctggatgagc ccgagcgcca ggggatggtc gggcacccgt
     2881 agggccaccg aggggagcga ggcggtcacc aggtcgggga tgaggggccc cttgggcagc
     2941 accagcgtca gggggcccgg ccaaaatttc tcggccaacc gaacggcgtc ccgggggaac
     3001 cgcagggcca ggatctgggc ctggcgcagg tccgccacgt gcacgatgag ggggtcgaac
     3061 tggggccggt ccttggcctc gaagacccgg gccacggcca tgacattggt ggcgtccgcg
     3121 cccaggccgt agacggtctc ggtggggaag gccaccaacc cgccttcctg caggatccgc
     3181 gcggcttttt cgatctcaga agtcacaata ggaccttcgt tctttgtttt atccgttttt
     3241 gatggaaggg gaacaaggcg cgccccaggc gcggcgtggg attgtttccc ttttacgttc
     3301 ttttgcgggc acggaaggcg gcccgcaaaa cgtcagcccg tgttcttgag gcccgccgcg
     3361 atgccgttga tggtcaacag gatggcgtcc tccaggtggg atttctcctc ctcggagggg
     3421 tggttgcggg cgcggcgcag cagctcgatc tggatgaagc tcaagggatc gatgtagggg
     3481 ttgcgcagct tgatggactg ggacagggcg ggttcccgct cgagcatttc ccccacctgg
     3541 gtgacctggc ggatgaccga gaccgtgcgg ttgtactcgc cctcgatgcg gccgaagatc
     3601 tcccggccca gcacggcgtc cggcaccagc tcggcgtagc ggcgcgcgat gtccatgtcg
     3661 gccttggaca ggatcatctg ggtgttgtcc agcaggcgct ggaagaaggg ccactggccg
     3721 tacatttcct gcagcacggc caggccttgc gggtcccctt tcaggtattc ctcgatggcg
     3781 gtgcccgccc cgaaccagcc ctggatggtg tggcggctct gcatccagct gaagacccag
     3841 gggatggccc gcaggtcctc aatgctgcgc ttggcgctgc ggctggaggg ccggctggcg
     3901 atgcggtgct ggctgatctc gttgatgggc gtggccgagg agaaatattc caggaaccgg
     3961 gggtggtcgt agatcaggcc ccggtaggcc ttgaaggaaa cggcggccag cttgtccatc
     4021 accagttccc agttgcggcg gggcaccact tcccggggcg ggaaggaacc gcgcaacacg
     4081 gcgttcacga tctgttccaa atggccccgg gcgttgtccg gctcgctgta gaggtcgctg
     4141 atgacctcgc cctgttcggt gatcttgatg ctgccccgga tggtcttggg cggctgggcc
     4201 aggatggcct tgttggcggg cccgccgccc cggccgatgc tgccgccccg gccgtggaag
     4261 aggcgcaggg tcacctggtg gttgtcggcc acccgcacca attcgacttg ggcgcggtag
     4321 agggcccagt tggcggccag gtagccgcct tccttgttgg aatcgctgta gcccagcatg
     4381 acctcctgct gttggttgcg gaactccagg tgcttgcggt agatggggat gttgaagagg
     4441 cggtccagga actggccggc gttctgcagg tcctggctgg tctccaagag cggcaccacg
     4501 ttcaggcggc tgtaggaatc gggcttgaag aggcccgctt cccggaaaaa gagcagcacg
     4561 cccagcacgt cgctgatgtt cttggtcatg gagatgatgt agctctcggc ggcctgggaa
     4621 tccaggtgct ccagcacgtg gttggccatg cggaaggtct cgatggtctc cagggtctcg
     4681 ggggtgaagg gcgtgcgctc ggggatgagg ggccggtggc tttccagctc cttcacgatc
     4741 agcgcgatct tctcctcctc gggcagcgac aggtagtcgt cggccaggcc cacgtacttg
     4801 aagatctcgg ccagggcggc ggcgtggcgc ccgctgtgct ggcgcaggtc cagcttggcc
     4861 agctgcagcc cgaagacccg gacccgggtg cggatgtcct ccaggaaccc gtcggccagc
     4921 acggagccct tgtgcgaccg gaggctctcg tccatcacgt ccaggtcctc catcagctgg
     4981 cccgcgtggg cgtaccaggt gcccggggcg ggggcgccgt cgtcgcccgg ggtgacgccg
     5041 tcgtagtccg cggtgtgggc catggtgcgg cgcagcttct cgtagatgaa gctgcatttt
     5101 tgccggtaca tctcattggg cgtgcgctgg ttgagccggt cggccaggtc ggggaaaagc
     5161 agcgaatcca gctccagcga ctggcgcagg cggtcgctga tgggcacctg gcggcaggaa
     5221 gagctcagcc ggtcgctgag catctccatg gaggtggaat aaaaatggag ggcgcagcgc
     5281 cgcatgacct tcagggtctc cacggtgact tcgggggtca cgaaggggtt gccgtcccgg
     5341 tccccgccca tccaggagcc gaaggtcagg aaggggcgga tgtcccaggc ggtcttgggg
     5401 aactcctcct gcagggattt ttccagctca cggtacagtt tgggcacgac cttgaagagg
     5461 gtctcctcga agtagtacag gtggcccttc acctcgtcca gcacgtcggg cttgaactgg
     5521 cggatctcgt cggtctgcca cagcgacacc agctcctcca cgatgtgctg catccggtcc
     5581 tggcgttcct cgggcagggc gtcctcgctc accatccggc gggcggattt ggcgatgcgg
     5641 tgcagctttt ccagcgtggt gggccgcttg gactcggtgg gatgggcggt gaagaccatg
     5701 gtgatgcggg ccaggtcgag cagcccctgg atcttcttgg gggggatgcc ctgtttgcgc
     5761 agggcctgga tggcggcggg gatggacccc tcgcgcttgg cctgggggtc cttctgggcg
     5821 gcgtcgcggg cctggtggat ctggtcgagc ttctccgcca ggttgatcag gccgaaataa
     5881 tgggtgaagg ccttggtgag ctggttgagc ttgtccaccg acaggcggga aaagatctgg
     5941 ttcaaggcct ccacgatggc cggcgagggc tgggcgcgca ggtccttgca gagcacgcgc
     6001 accttttcca cccgggccag ggtgtcgggc ccttcctggt cggcgatgac ctggcccagg
     6061 gtgcccgcca gcacccgcac caggtggggc agggcgtagg agtcggcctt cggcatggcc
     6121 gccaggtcca gttgtttggt ctcttcttgg aacatgatca ggaaccaccc caaaccgatt
     6181 taccgcaaag gcgccaagcc gcaaaaggaa cgaccacccc gggagggggc ggccttaaga
     6241 ccggccttcg gtgcctttgg tcttgcggcg aaagggtctt aagacaattt ttcc
//
LOCUS       DASTJJ010000078         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_622611, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000078 DASTJJ010000000
VERSION     DASTJJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622611"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..620)
                     /locus_tag="VFR02_00955"
     CDS             complement(171..620)
                     /locus_tag="VFR02_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869052.1"
                     /translation="MKLFRVSFWPVFFCVFVGASPIVLLAFMDRLLWSSLILRMLLSL
                     CGTGIPISLFTAWIYWQYLPVRVSPEGVTGTSFWGFPKTTGWDDIRAVKPIRILNLRW
                     LRVYPKDGTGANWVALFVKDFKTFKTEVRDAHPSGNLFGYELEKIKP"
     gene            711..1145
                     /locus_tag="VFR02_00960"
     CDS             711..1145
                     /locus_tag="VFR02_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869053.1"
                     /translation="MKSWVWVVLAGLALGPAGAEEPLPTPTPTASPSASATPSCAAPG
                     ASAASGDARNYTAWCAPGLPGVIFEQDDANNGVAYSVFNGTDVPVDVRLGAKSLVNMD
                     PNLRDGWVRVAPRKQEDLGKLTAHDPNWSWSASVTATAQPAP"
     gene            complement(1258..1539)
                     /locus_tag="VFR02_00965"
     CDS             complement(1258..1539)
                     /locus_tag="VFR02_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869054.1"
                     /translation="MIQVEQPLSIPQIFDSSVALTTNQDCPAALSSESPLERQPEATA
                     SALTAVPADPPGFVGMELGLQAIPKATTRTRSPIEIKDFTRATKIVGFK"
BASE COUNT          337 a          489 c          552 g          320 t
ORIGIN      
        1 ggaataccgg gtggccctgc tgccgctctc ggacgccgaa gccacggtgc cgggctattt
       61 ttacggggac ggcgggacct tgcgggccca ggacggcgcg gaggggacaa agatctttta
      121 ttcgggctat tggttccgaa aggccgggga acaggccggc ccctgagggg tcagggtttg
      181 atcttttcca gctcgtagcc aaagaggttg ccgctggggt gggcgtcccg cacctcggtc
      241 ttgaaggttt tgaaatcctt gacgaagagc gccacccagt tggccccggt gccgtccttg
      301 ggatagaccc gcaaccaacg caggttcagg atgcggatgg gcttcacggc ccggatgtcg
      361 tcccaaccgg tggtcttggg gaaaccccag aaagacgtgc ccgtcacccc ctccggggag
      421 acccgcacgg gcaggtattg ccagtagatc caggcggtga agaggctgat ggggatgccg
      481 gtgccgcaga gggacagcag catgcggagg atcaaggacg accagagcag gcggtccatg
      541 aaggcaagga gtacgatggg gctggccccc acgaacacgc aaaagaagac gggccaaaaa
      601 gagacgcgga acagcttcat gggacacctc cggacggccc cattttattc cagcgggccg
      661 ggggaattcg gtttattttc aaaggagagg ccttcatgga acaaaaaggg atgaaatctt
      721 gggtttgggt ggtgttggcc ggcttggcct tggggcccgc cggggccgaa gaacccctgc
      781 cgacccccac ccccaccgcg tcgccgtcgg cctcggccac gccttcctgc gcggcgcccg
      841 gcgcctccgc ggccagcggg gacgcgcgca attacaccgc ttggtgcgcc cccggcctgc
      901 cgggggtgat cttcgagcag gacgacgcga acaacggggt ggcctatagc gtcttcaacg
      961 gcacggacgt tcccgtggac gtgcgcctgg gcgcgaagtc cctggtcaac atggacccca
     1021 acctgaggga cggctgggtg cgggtggcgc cccgcaagca ggaggacctg gggaagctga
     1081 ccgcccacga ccccaactgg tcctggagcg ccagcgtcac cgccacggcc caaccggcgc
     1141 cttagggcaa gagcttttgc actttttgtg acattttaat tcatctgttt gtcaaatttt
     1201 atttattcgg gttgattatc gaagggggct tatctatgat ccgaggcatt ggcttaatta
     1261 tttaaatcca actattttgg tggcgcgtgt gaaatccttg atctcgatcg ggctccttgt
     1321 tcttgtggtc gctttgggga tagcttgcaa tcccagctcc atgccgacaa agcccggtgg
     1381 atctgcaggg accgccgtca gggccgaggc ggtcgcctcg ggttgcctct caagcggcga
     1441 ttcgctggac aacgcggccg gacaatcttg gttggtggtc agcgctactg acgaatcaaa
     1501 tatttgtggt attgataacg gttgttccac ctggatcacg aacccggcct ccccgggcag
     1561 ggatccgggc ccggtggtcg atcacagcaa cgaaaatgac cggatccaag ctgacctgcc
     1621 ccccaaaacc tggtccagaa ataaagtcac agttggcttt agaatgggcc gacaaagggg
     1681 gccaatatgg cacgcaag
//
LOCUS       DASTJJ010000079         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_868581, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000079 DASTJJ010000000
VERSION     DASTJJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868581"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFR02_00970"
     CDS             <1..720
                     /locus_tag="VFR02_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9869055.1"
                     /translation="VSAVMAWTGFGNVGVDVFLFVVSGWLVSLCLHEYGHAWAAYRLG
                     DPTAKMLGRLTLDPRAHVDPIGTLLFPMLRLLYPGLFLLGWAKPVPVTAENFRHPKRD
                     NALVAAAGPAMNLVLAFLALVVLGVTAAAGFFGLSAQTGGTLFEFLHFFFWLNFGLAL
                     FNLFPLYPLDGNWILKALLPDRLSYAYSRLDRFGIWAFLAVVVFFPSVFSQLFLPAVA
                     GLYWMLHLAGLDGLAQMLNLG"
     gene            755..1819
                     /gene="trpS"
                     /locus_tag="VFR02_00975"
     CDS             755..1819
                     /gene="trpS"
                     /locus_tag="VFR02_00975"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9869056.1"
                     /translation="MTSETNPKKPTRILSGVQSNGRLHLGNYFGAIQNHIRLQDEGEC
                     FYFIANYHSLTTLQSAPDLAKYTFMAAADYLALGLDPAKALLFRQSDVPEVTELAWIL
                     STVTGMGLLERAHSFKDKIARGIAPSMGLFFYPILMAADILIYQSEKVPVGQDQVQHV
                     EMTRDMAGHFHQHYAKGVFTLPEAVLSQTPKVMGLTGGKMSKSDEASVLPIFGEPAEI
                     KKKVMSIITDSKGVDEPKDPEGNVIYLLYKLLASPEEAAQMAEGFRKGGMGYGDAKKT
                     LLAQLEKSFGGETREKRKAYDAHPDRVEDVLVEGAKKARRAAAATMDRVYAATGIAAS
                     RIKRKMSDLDLEAPNNGLGK"
     gene            1993..>2229
                     /locus_tag="VFR02_00980"
     CDS             1993..>2229
                     /locus_tag="VFR02_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869057.1"
                     /translation="MTDEKPTTEGGAEAAPEPVTVALSKEVTGVVIKEVLHSEDQRLT
                     YQITLPSFDGPMDLLLHLIKEHELDIYDIPISKIT"
BASE COUNT          410 a          726 c          683 g          410 t
ORIGIN      
        1 gtcagcgccg tcatggcctg gaccgggttc ggcaacgtcg gcgtcgacgt cttcctgttc
       61 gtggtcagcg gctggctggt ctcgctctgc ctgcacgaat acggccacgc ctgggccgcc
      121 taccggctgg gcgaccccac ggccaagatg ctggggcggc tcaccctgga cccccgggcc
      181 cacgtggacc ccatcggcac cctgctgttc cccatgctgc gcctgctcta tccgggcctt
      241 tttcttttgg gctgggccaa gcccgtgccc gtgaccgccg agaacttcag gcatcccaag
      301 cgggacaacg ccctggtggc cgccgcgggc cccgccatga acctggtcct ggccttcctg
      361 gccctggtcg tcctgggcgt cacggcggcc gcggggttct tcgggttgtc cgcccagacg
      421 gggggcacgc tcttcgagtt ccttcatttc ttcttctggc tgaacttcgg cctggcgctc
      481 ttcaaccttt tcccgctgta tccgctggac gggaactgga tcctgaaggc cctgttgccc
      541 gaccggcttt cctacgccta ttcgcgcctg gaccgtttcg gcatctgggc cttcctggcc
      601 gtggtggtct ttttcccctc ggtgttcagc cagctgttcc tgccggcggt ggccggcctt
      661 tactggatgc tgcacctggc ggggctggac ggcctggccc aaatgctcaa cctgggctga
      721 ggcccgggca ccccaaggat cggacgaggc gaacgtgacg tcggaaacca acccgaaaaa
      781 acccacccgc atcctgtcgg gcgtgcagtc caacgggcgg ctccacctgg gcaactattt
      841 cggggccatc cagaaccaca tccgcctgca ggacgagggg gagtgctttt atttcatcgc
      901 caactaccat tcgctgacca ccctgcagtc cgcgcccgac ctggccaagt acaccttcat
      961 ggcggccgcc gattacctgg cgctggggct cgacccggcc aaggcgctgc ttttccgcca
     1021 aagcgacgtg cccgaggtga ccgaactggc ctggatcctc tccaccgtca ccggcatggg
     1081 gctcttggag agggcccatt cgttcaagga caagatcgcc cggggcatcg caccctccat
     1141 ggggctcttc ttttacccca tcctgatggc cgccgacatc ctcatctacc agagcgagaa
     1201 ggtgcccgtg ggccaggacc aggtgcagca cgtggagatg acccgggaca tggcgggcca
     1261 tttccaccag cactacgcca agggcgtctt caccctgccc gaggcggtgt tgagccaaac
     1321 gcccaaggtc atggggctca ccgggggcaa gatgagcaag agcgacgagg ccagcgtgct
     1381 gcccatcttc ggggaacccg ccgagatcaa gaagaaggtc atgtccatca tcaccgactc
     1441 caagggcgtg gacgagccca aggaccccga ggggaacgtc atctaccttc tttataagtt
     1501 gctggcaagt ccggaagagg cggcccaaat ggccgagggt ttccgaaagg gcggcatggg
     1561 ttacggcgac gccaagaaga ccctgctggc ccaactggaa aagagcttcg gcggggaaac
     1621 gcgggaaaaa cgaaaggcct acgacgccca tccggaccgg gtggaggacg tgttggtgga
     1681 aggcgcgaaa aaggcccgcc gggccgccgc ggcgaccatg gaccgggtct acgccgccac
     1741 aggcatcgcg gcttcccgga tcaagcgtaa gatgtcggat cttgatcttg aagcgcctaa
     1801 caacggattg ggaaaataaa ggctgatttt caccaccaag gcaccaagac accaagaact
     1861 gctgatggcc atttgacggg atttgggtcc ccttggtgcc tgccccaaca ttgatgtttg
     1921 ggtaacggag ccacccttgg gtgtggtggt gaggatgtga tcttgagatg tgcttggtgt
     1981 taaagaggga atatgacgga cgagaaaccc acaaccgaag gcggggccga ggcggccccc
     2041 gaacccgtga ccgtggcgct gtccaaggaa gtcacgggcg tggtcatcaa ggaggtcctc
     2101 cattcggagg accagcgcct gacctatcag atcaccctgc cttccttcga cgggcccatg
     2161 gaccttttgc tccacctcat caaggagcac gagctcgaca tctacgacat ccccatctcc
     2221 aagatcacc
//
LOCUS       DASTJJ010000080         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_501149, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000080 DASTJJ010000000
VERSION     DASTJJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501149"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..622
                     /gene="rnpB"
                     /locus_tag="VFR02_00985"
     ncRNA           187..622
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFR02_00985"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            795..>1001
                     /locus_tag="VFR02_00990"
     CDS             795..>1001
                     /locus_tag="VFR02_00990"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9869058.1"
                     /translation="MQKIRIRGGKKLNGTVDISGSKNATLPVMTAALLAKTPTTLTNV
                     PHLNDIKTMAEVLRRLGAKVLLQDH"
BASE COUNT          206 a          292 c          304 g          199 t
ORIGIN      
        1 cgacgaacac caaactaaaa attagttcac caccaaggca ccaagacacc aaggtagtct
       61 tttaattctt tgtcgacttt tcgggttttc ttggtgtctt ggtgccttgg tggtgaaaat
      121 aggtgttttt ggggtgtttc gttctggcgc tggatttaag ccgcttttcc ccctatgatt
      181 gcccctggaa gcaggtccgt gatcgcggcc gttcgggatt cccgtccggt cgaggaaagt
      241 ccgagctcca gaggacagga tgccgggtaa cgcccggtcg ccacgccccc gcaaggggaa
      301 cggggtgaag gaaagtgcca cagaaacaaa ccgcccttcg acaggctcag ggcgcgcctc
      361 cgcaatgggg ctcgccccga gcttgtcgag gggtaagggt gaaaaggtgg ggtaagagcc
      421 caccgcgtcc cgcgcaagcg tggcggccag gcaaacccca tccggagcaa gatcaaatag
      481 gagggcttcg gggtcgtccg ccccacgggt ttcccaacgg aaaaccgggc cttcgggtaa
      541 gatcgctgga cggggccggt gacggtcccc gcgagagaaa tgatcacatc tacagaactc
      601 ggcttatgcc ctgcttcctt caacggaccc taatataggg tatgttaggc cccattggtt
      661 tggaccgatg gggccttttt ctggcccgga tgtatctcgg actttcttgg cgtctccgcg
      721 tctgccccaa cattaaattg gggggaacgg ggccgccctt gggcgtggcg tgcgccgtac
      781 ttttgggggt tcttttgcaa aagatcagga tccggggcgg taaaaaactg aacgggaccg
      841 tggacatcag cggctcgaag aacgccacgc tccccgtcat gacggcggcg ctcctggcca
      901 agacgcccac caccctcacc aacgtgccgc acctcaacga catcaagacc atggcggaag
      961 tgctgcgccg cctgggcgcc aaggtgctcc ttcaagacca c
//
LOCUS       DASTJJ010000081         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1357317, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000081 DASTJJ010000000
VERSION     DASTJJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357317"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1997)
                     /locus_tag="VFR02_00995"
     CDS             complement(<1..>1997)
                     /locus_tag="VFR02_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9869059.1"
                     /translation="EKFGQDELGELTDAFNQMLAEIQGQAQALKASEANYREIFEKAN
                     DGIIITDPHTTQNPILDMNASLERMTGFDREEYKLVPAEKLFSDKPGSTAADYALLAQ
                     KAMAGEPQVFEWQALHKDGHTYWIEVSLKRATLAGQTRLVAFMRDIQDRKKLEAITRL
                     GEAQAIESIKDYAIVMLDPEGRILTWNRGAEAIKGYGRQEVLGKPVSLFYTPEDRKAG
                     VPERNLRTAADKGQMETEGVRVRKDGSRFEADVILTALRDDKGDLRGFIKVTRDVTQV
                     RRDERELREKSELLDLVLKNIADGVVVADEKGRFLLFNPAAEKISGRPGEGAQPGQWT
                     EQFGIFLPDGKTPFPESDNPLAQAVTGKATDNVEMFLRNPGHPEGIRVSVSSRPLKDE
                     KGRIRGGVAIFRDVTAQKRSEDEIKQANLFLNAVLENLPNMVFVKDAKDLRFVMFNQA
                     GEDLIGVSRSDLLGKNDHDLFPKDQADFFTGQDRRTLEGGRLVDIPEETIQSKAKGKR
                     LLHTRKIPVKGPDGAPRYLLGISEDITEQKQQETLRIYAKALEASNKDLQDFIFVASH
                     DLQEPLRKIQAFGNFLDQEDGKALSPTGKDYLARIRDASQRMSTLLSDLLDLTRVTTR
                     AKPFVPVDLNEIVREVVSDLEIRLEETGGLVEADGLPKVDA"
BASE COUNT          268 a          669 c          645 g          415 t
ORIGIN      
        1 cggcgtccac tttgggcagg ccgtcggcct ccaccagccc ccccgtctcc tccaggcgga
       61 tctccaggtc gctgacgacc tcccggacga tctcgttgag gtcgacgggc acgaagggct
      121 tggcccgggt ggtgacccgg gtcaggtcca gcaggtcgga caagagcgtg ctcatgcgct
      181 gggaggcgtc ccggatacgc gccaggtagt ccttgcccgt gggcgagagc gccttgccgt
      241 cctcctggtc caggaagttg ccgaaggcct ggatcttgcg aagcggctcc tgcaggtcgt
      301 gggaggccac gaagatgaag tcctgcaggt ccttgttgct ggcctccagg gccttggcgt
      361 agatgcgcag ggtctcctgc tgtttttgct cggtgatgtc ctccgagatg cccagcaggt
      421 accggggcgc gccgtcgggc cccttgacgg ggatcttgcg ggtatggagc aaccgcttgc
      481 ccttggcctt gctttggatc gtttcttccg ggatgtccac cagccgcccc ccctccaggg
      541 tcctgcggtc ctggccggtg aagaaatccg cctggtcctt ggggaagagg tcgtggtcgt
      601 tcttgcccaa aaggtcggac cgggagacgc cgatcaggtc ctcgcccgcc tggttgaaca
      661 tgacgaaacg caggtccttg gcgtccttca cgaagaccat gttgggcagg ttctccagca
      721 ccgcgttcag gaacaggttg gcctgcttga tctcatcctc ggaccttttc tgcgccgtca
      781 cgtcccggaa gatggccacc ccgccgcgga tgcgcccctt ctcgtccttc agggggcggc
      841 tcgagacgct gacccggatg ccctccgggt gtcccggatt gcgcaggaac atctccacat
      901 tgtcggtggc cttgccggtg accgcctggg ccaacgggtt gtcgctttcg gggaaggggg
      961 tcttgccgtc gggcaggaag atgccgaact gctcggtcca ctggccgggc tgggcgccct
     1021 ccccgggccg gcccgagatc ttctcggccg ccgggttgaa gagcaggaac cggccctttt
     1081 catcggccac caccacgccg tcggcgatgt tcttcagcac caggtccagc agttcgctct
     1141 tttcgcgcag ttcccgctcg tcccggcgaa cctgggtcac gtcccgggtg accttgatga
     1201 agccccgcag gtcccccttg tcgtcgcgca gggcggtcag gatcacgtcg gcctcgaacc
     1261 ggctcccgtc cttgcggacc cggacaccct cggtctccat ctgccccttg tccgcggccg
     1321 tccggaggtt cctctcgggc acgccggcct tccggtcctc gggcgtatag aagagggaga
     1381 cgggcttgcc caggacctcc tgtcggccat agcccttgat ggcctccgcc ccccggttcc
     1441 aggtcaggat ccggccttcg gggtccagca tgacgatcgc atagtccttg atgctctcga
     1501 tggcctgggc ctcgcccagg cgggtgatcg cctccaactt tttacggtcc tggatgtcgc
     1561 gcatgaaggc caccagccgg gtctggcccg ccagggtggc ccgcttcaag ctcacctcga
     1621 tccaataggt atggccgtcc ttatgcaggg cctgccactc gaagacctgg ggctcccccg
     1681 ccatggcctt ttgggccagc agggcgtagt ccgccgcggt ggacccgggc ttgtccgaga
     1741 agagcttttc cgccggcacc agcttgtatt cttcccggtc gaacccggtc atcctctcca
     1801 ggctggcgtt catgtccagg atggggttct gggtggtgtg gggatcggtg atgatgatgc
     1861 cgtcgttggc cttctcgaag atctcccggt aattggcctc ggaggccttg agcgcctggg
     1921 cctggccctg gatctcggcc agcatctggt tgaaggcgtc cgtgagctcg cccagttcat
     1981 cctgcccgaa tttttcc
//
LOCUS       DASTJJ010000082         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1357362, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000082 DASTJJ010000000
VERSION     DASTJJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357362"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFR02_01000"
     CDS             complement(<1..516)
                     /locus_tag="VFR02_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869060.1"
                     /translation="MTSRVPKPLMVFALAGLLLSFSAPRLYAQSDLGLAGSLEANHQT
                     HFYKDPTKAFLLAFFPGFLIHGYGHFYAEDNLMGATLLTGEVLSLASIGTGLIIKSDT
                     STFSGGILGSPANADQAGSDMIIGGCILFAVTWVADMAHAPTSAEDYNHEFNLEPVAS
                     LSGHRVDLALAC"
     gene            complement(645..1097)
                     /gene="rplQ"
                     /locus_tag="VFR02_01005"
     CDS             complement(645..1097)
                     /gene="rplQ"
                     /locus_tag="VFR02_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9869061.1"
                     /translation="MRHAAHHGRLGVQPAHRRAMIRNMVTSLLKHERIRTTKARAKEV
                     RRYAEKMITTAKKDTLAAKRQVLGFVREREVVNKLFKTLIYRYAERKGGYTRILRLAP
                     RPGDGADMVFLELVDRAVPEKTEAPKGDKKAETAAPEAPKKKETAASA"
     gene            complement(1125..2135)
                     /locus_tag="VFR02_01010"
     CDS             complement(1125..2135)
                     /locus_tag="VFR02_01010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9869062.1"
                     /translation="MQIGNVQIPKFLDYEKESLSPTYGKFIAEPFERGYGQSVGNSLR
                     RILLSSITGAAVTSVKFAGVSHEYSSIPGVIEDTTEIILNLKELKIKLFGNGPKTLLL
                     SVKGERNVVAADIKADADVEILNPQLHLATLTEDDASLEVEIEVAEGRGYSPSERNKR
                     EGQSIGVMPVDSIFTPVSQVKYSVESARIGQVTDYDKLIIEIWTDGRIHPEDAMAHAA
                     KILRDSLQIFINFEEEPVTQDDSVSEEEERLRELLSESVEELELSVRSANCLKTANIK
                     TIGDLVRKTESDMLKYKNFGRKSLNEIKEILNGMGLSFGTDVDGILAKGKSGMARVEV
                     KV"
     gene            complement(2162..2782)
                     /gene="rpsD"
                     /locus_tag="VFR02_01015"
     CDS             complement(2162..2782)
                     /gene="rpsD"
                     /locus_tag="VFR02_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9869063.1"
                     /translation="MSVQAVCRICRREGMKLYLKGTRCESEKCAFERRSYAPGQHGQK
                     RRKDTEYGLQLREKQKVKRIYGILEKQFRRYFSIASRKKGVTGETLLQLLEQRLDNTV
                     YRLGFAESRRQARQVVRHGMVSVNGKKVDIPSYTLKAGDKIAIQDKFKENLVLKRSVE
                     SLAKRGVPEWLEVDATALAGSFKKVPTKDEIALPVQEQLIVELYSK"
     gene            complement(2795..3193)
                     /gene="rpsK"
                     /locus_tag="VFR02_01020"
     CDS             complement(2795..3193)
                     /gene="rpsK"
                     /locus_tag="VFR02_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9869064.1"
                     /translation="MKDEKAAPSAAAKKGKKAPRNGVVHVQCTFNNTLVTVTDGKGNV
                     VAWSSAGSMNFKGSRKSTPFAAQMAAEAAGKKALDAGVKSVEIYVKGPGSGREAAIRA
                     IQTCGLQISLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(3283..3663)
                     /gene="rpsM"
                     /locus_tag="VFR02_01025"
     CDS             complement(3283..3663)
                     /gene="rpsM"
                     /locus_tag="VFR02_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9869065.1"
                     /translation="MARIAGIDLPKEKRVDVALTYLFGVGPALSKKILAKAGVNPSVR
                     VKNLKDDEVARIQNTINNDGIKVEGDLRREISMNIKRLIDIGTFRGSRHRKGLPVRGQ
                     RTKTNARTRKGPRKTVGSGRKKEE"
     gene            complement(3677..3790)
                     /gene="rpmJ"
                     /locus_tag="VFR02_01030"
     CDS             complement(3677..3790)
                     /gene="rpmJ"
                     /locus_tag="VFR02_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9869066.1"
                     /translation="MKVRSSVKPICAKCKVIRRRRVVRVICTNPKHKQRQG"
BASE COUNT          610 a         1177 c         1202 g          834 t
ORIGIN      
        1 gcaggccagg gcgaggtcca cccggtgccc ggaaagggag gccaccggtt ccaggttgaa
       61 ctcgtggttg tagtcctccg ccgaggtcgg ggcgtgggcc atgtcggcca cccaggtgac
      121 cgcgaagagg atgcagcccc cgatgatcat gtcgctcccc gcctggtcgg cgttggccgg
      181 gctgcccaag atgccgcccg agaaggtcga ggtatcggac ttgatgatga gccccgtgcc
      241 gatggaggcc agggaaagca cttccccggt caggagggtg gcgcccatca ggttgtcctc
      301 ggcgtaaaaa tggccgtagc cgtggatcag gaagccggga aagaaggcca gcaggaaggc
      361 cttggtgggg tccttataaa aatgggtctg gtggttggct tccaggcttc ccgccaatcc
      421 caggtcggac tgggcgtaaa gccgcggggc tgaaaacgat aaaaggaggc cggccagcgc
      481 aaaaaccatg aggggcttgg gaacgcgtga ggtcataagt ggactcattt taagggaggc
      541 cccgggcgag gtcaaaagaa aagacccgcc ccggccggat ttagggcccc ttcggggcca
      601 aaaagaaaag ggggccccaa gggccccctt ccccaaacca tgcgtcaagc ggaagcggcg
      661 gtttccttct ttttcggggc ctcaggcgcg gccgtctcgg ccttcttgtc gcccttgggg
      721 gcctcggtct tctcgggcac ggcgcggtcc accagttcca ggaagaccat gtcggcgccg
      781 tcgcccggcc ggggggccag ccgcaggatg cgggtataac cgcccttgcg ctcggcgtag
      841 cggtagatga gggtcttgaa gagcttgttc accacttccc gctcccgcac gaagcccagc
      901 acttgccgct tggcggccag ggtgtccttc ttggccgtgg tgatcatctt ctcggcgtac
      961 cggcgcactt ccttggcccg ggccttggtg gtgcggatgc gctcgtgttt caggagggac
     1021 gtgaccatgt tgcggatcat ggcccggcgg tgggccggct ggacgcccaa acggccgtgg
     1081 tgtgctgcgt gtcgcatgac ttatcctctt ctttctatgg ttcgttaaac tttgacttcg
     1141 acccgggcca tgcccgactt gcccttggcc aagatgccgt ccacgtcggt cccgaaggac
     1201 aggcccatcc cgttgaggat ctccttgatc tcgttgagcg acttgcggcc gaaattcttg
     1261 tacttgagca tgtcggactc ggtcttgcgg accaggtcgc cgatggtctt gatgttggcg
     1321 gtcttcaaac agttggccga gcgcacggac agttccagtt cctccacgct ctcgctcaac
     1381 agctcgcgca gccgttcctc ttcctcggac accgagtcgt cctgggtgac gggctcttcc
     1441 tcgaagttga tgaagatctg cagcgaatcc cgcaggatct tggccgcgtg ggccatggcg
     1501 tcctcggggt ggatgcggcc gtcggtccag atctcgatga tgagcttgtc gtagtccgtg
     1561 acctggccga tgcgggccga ttccaccgag tacttcacct gcgagaccgg cgtgaagatg
     1621 gaatccacgg gcatgacgcc gatggactgg ccctcgcgct tgttgcgctc ggacggcgag
     1681 tagccccggc cctcggccac ctcgatctcc acttccagcg aggcgtcatc ctcggtcagc
     1741 gtggccaggt gcagctgggg gttcaggatc tccacgtccg cgtcggcctt gatgtcggcg
     1801 gccaccacgt tgcgctcacc cttgacggac aagagcaggg tcttggggcc gttgccgaag
     1861 agcttgatct tcaactcctt caagttgagg atgatctcgg tcgtgtcctc gatcaccccc
     1921 gggatggagg aatactcgtg ggaaacgccc gcgaacttca ccgaggtcac cgccgcgccc
     1981 gtgatggacg acagcaggat gcggcgcagc gagttgccca cggactgccc atagccgcgc
     2041 tcgaaaggct cggcgatgaa cttgccgtag gtgggcgaca ggctttcctt ctcgtaatcc
     2101 aaaaacttcg ggatctgaac gtttccgatt tgcataaccc ctccccttga aataggtcct
     2161 gttacttgga gtaaagttcg acgatgagct gttcctgcac gggcagggcg atctcatcct
     2221 tggtgggcac cttcttgaag gagcccgcga gagcggtggc gtccacttcg agccactcgg
     2281 ggacgccgcg tttcgccagc gattccaccg agcgcttcag gaccaggttt tccttgaact
     2341 tgtcttggat ggcgatcttg tccccggcct tcaaggtata ggaggggatg tccactttct
     2401 tgccgttcac cgacaccatg ccgtggcgca ccacttggcg ggcctggcgc cgggactcgg
     2461 cgaagcccag gcggtacacc gtgttgtcca ggcgctgttc gagcaattgg agcagggtct
     2521 cgccggtcac acccttttta cgggacgcga tggagaaata gcggcggaac tgtttttcca
     2581 ggatgccgta gatgcgcttg accttctgct tttcgcgcaa ctgcaggccg tactcggtgt
     2641 ccttccggcg cttctggccg tgctggcccg gggcgtagct gcggcgctcg aaggcgcact
     2701 tctcgctctc gcagcgggtc cctttcagat aaagtttcat gccttcccga cggcagatgc
     2761 ggcaaacggc ttgaacgctc atggatcggc tcctttacac gcgccggcgc ttgggcggcc
     2821 ggcaaccatt gtgggggata ggggtgacgt ctttgatgag ggagatctgc agcccgcagg
     2881 tctggatggc gcggatggcc gcttcccgtc ccgaacccgg gcccttgacg tagatctcaa
     2941 cggacttcac gcccgcgtcc agggccttct tgcccgccgc ttcggcggcc atctgggccg
     3001 cgaagggggt cgatttgcgg ctccccttga aattcatgga gcccgcggag gaccaggcca
     3061 ccacgttccc cttgccgtcg gtcacggtca ccagggtgtt gttgaaggtg cactggacgt
     3121 ggaccacgcc gttccggggc gccttcttgc cctttttggc ggcggccgaa ggggccgctt
     3181 tctcgtcctt cacttcgatc ttgggttcgg ttttcttgac ttcgtcagcc actcgttttc
     3241 tccttcaatt tcaagtccgt ccgctcgcgc gtcgtccctg gtttattctt ccttcttgcg
     3301 gccgctgccg accgtcttcc gcgggccttt gcgggtacgg gcgttggtct tggttcgttg
     3361 gccccggacc ggcagccctt tccggtggcg gctgccccgg aacgtgccga tgtcgatcaa
     3421 acgtttgatg ttcatgctga tctcgcggcg caggtcgccc tcgaccttga tgccgtcgtt
     3481 gttgatggtg ttctggatgc gggccacctc gtcgtccttc aggttcttca cccggacgct
     3541 ggggttcacc ccggccttgg ccaggatctt cttggaaagg gccggaccca ccccgaacag
     3601 ataggtgagg gccacatcca cccttttttc cttcggcaaa tcaattccgg caatgcgagc
     3661 catacgttct cctcaattat ccctgacgtt gtttgtgctt cggattcgtg caaatgacgc
     3721 ggaccacccg ccggcggcgg atgaccttgc atttggcaca gatcggcttc acggacgacc
     3781 gaaccttcat gtctctttcc ttcctaaatc ctcggactta cga
//
LOCUS       DASTJJ010000083         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_379206, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000083 DASTJJ010000000
VERSION     DASTJJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379206"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2361
                     /locus_tag="VFR02_01035"
     CDS             <1..2361
                     /locus_tag="VFR02_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9869067.1"
                     /translation="LDPFLEAVEFPQVLRRLAEGCATPAGRENLTAFRPLAEGEALAG
                     RLARTEQLEQQVIRHGEPPVPSPEDFEPAFLESVRTGRSFSAAELGALLRFLDGMARL
                     RRFLSLGDGPAPALAAWLARLGAFPALKDFLSARVSDQGDVRDGASPELKSARGSLRA
                     LRAEVQEAYQRFLQGSGEALQEKLVTEREGRFVVPVKRDHQSQVPGFVHGTSASGATL
                     FVEPQALAERNHRLREAALREDEEVRRVLRACTEAVLAEAEGIQAALSACAEIDAHAA
                     LARFASRFEARFLAPAPGGAFRLVGARHPLLALEAGEAFREKVVPLDLDFSNGTRVLL
                     LSGPNAGGKTVALKTLGLCCAMAQAGLPVLAGGASSFPWLTRFDTDLSDEQDLEGHLS
                     TYAAKLTALKRMLDGCDGGTLLLLDELGSGTDPREGGALGLACLEAFREKGALVFANT
                     HQPLLKLLTQEEKGMANAAMLFDEATGKPTFKLVVGLPGRSYALTLATQLGFDPAVLE
                     RAKAHLPPGEADLSGLLASLAGEKQRAEEARREAEQARDRVKKTEAELLVARRQIKDE
                     ARRVRREAQIEAEGIVKNTRRQMDHLIQGVAPAGELDRGKVRQAQREVGRKLGNIPRA
                     AERPRVELRELKPGDAVHFKNGHSRAKVLEADDDKQTAVILLEGGLKLSCQYADLGPL
                     GPAPARAPLRPQPTALEKAKADKGALELDLRGRLVDQALPLLDKFIDDALLVDLPFVR
                     IIHGKGTGALKEAIHKHLPVQHPQVEFSLAEPAQGGAGVTVVRFKK"
     gene            2495..>3028
                     /locus_tag="VFR02_01040"
     CDS             2495..>3028
                     /locus_tag="VFR02_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HET9869068.1"
                     /translation="MKYSDDIIQRVRDAADIVSVVGEYVPLKKAGSSYKGLCPFHSEK
                     SPSFIVSPSRHSFHCFGCGKGGNVITFVMEMEKMPFPEALRHLAAKAGVPLPKPEETR
                     RDEAADRRRERMLALNELAGRLYHEKLLSPEGQAARDYYKGRGFTKEMAVKFQIGYAP
                     PGWDFLKNAAQKAGFSRE"
BASE COUNT          486 a         1029 c         1065 g          448 t
ORIGIN      
        1 ttggacccct tcctggaagc ggtggaattc ccccaggtac tgcggcgcct ggcggagggc
       61 tgcgccaccc ccgcgggccg ggaaaacctg acggctttcc gccccctggc cgaaggggaa
      121 gccctggccg ggcgcctggc ccgcaccgag caattggaac aacaggtcat ccgccacggc
      181 gagcccccgg tcccgtcgcc ggaggatttc gagcccgcct tcctggagtc ggtccgcacg
      241 ggccggtcct tttcggcggc cgaactgggg gcgctgctgc gtttcctgga cgggatggcg
      301 cgcctgcgcc gcttcctgtc gttgggggac gggccggccc cggccctggc cgcctggctc
      361 gcccggctgg gggccttccc ggcgttgaag gactttttgt ccgcccgggt ctcggaccag
      421 ggggacgtgc gggacggggc ctcgcccgaa ctcaaaagcg cccgcggcag cctgcgggcc
      481 ctgcgcgccg aggtccagga ggcctaccag cggttcctgc aggggtccgg cgaggccctg
      541 caggaaaagc tcgtcaccga gcgcgagggg cggttcgtgg tgccggtcaa gcgcgaccac
      601 cagtcccagg tgccgggttt cgtgcacggc acctcggcct cgggcgccac cctcttcgtg
      661 gaaccccagg ccctggccga gcgcaaccac cgcctgcggg aagccgccct gcgcgaggac
      721 gaggaagtgc gccgggtgct gcgcgcctgc acggaggcgg tcctggccga ggccgagggc
      781 atccaagcgg cgctttcggc ctgcgccgag atcgacgccc acgcagccct cgcccgtttc
      841 gcctcgcggt tcgaggcgcg gttcctcgcc cccgcccccg gcggcgcctt ccggctggtc
      901 ggcgcgcggc accccctgct ggccctggag gccggggaag ccttccggga aaaggtggtg
      961 cccttagacc tcgatttctc gaacggcacc cgggtgctgc tcttgtcggg ccccaacgcg
     1021 ggcggcaaga ccgtggccct gaagaccctg gggctatgct gcgccatggc ccaggcgggc
     1081 ctgcccgttt tggcgggggg cgccagttcc ttcccttggc tcacccgctt cgacaccgac
     1141 ctctcggacg agcaggacct ggaaggccac ctgagcacct acgcggccaa gctcacggcc
     1201 ttgaagcgca tgctggacgg ctgcgacggg gggaccctgc tgctcttgga cgaactgggg
     1261 tcggggaccg atccccggga aggcggcgcg ttgggcctgg cctgcctgga ggccttccgc
     1321 gagaaaggcg ccctggtctt cgccaacacc caccagccgc tcttgaagct gctcacccag
     1381 gaagagaagg gcatggccaa cgccgccatg cttttcgacg aggcgaccgg caagcccacc
     1441 ttcaagctgg tggtgggcct gccgggccgg tcctacgcgc tgaccctggc cacccaactg
     1501 ggcttcgacc ccgcggtgct ggaacgggcg aaggcccatt tgccgccggg tgaggcggac
     1561 ctgtcggggc tcttggcctc gctggccggg gaaaagcaac gggccgagga ggcccgccgg
     1621 gaggccgagc aggcccggga ccgggtgaaa aagaccgagg ccgaattgct ggtcgcccgg
     1681 cggcagatca aggacgaggc gcgccgcgtg cggcgggagg cccagatcga ggccgagggc
     1741 atcgtcaaga acacccggcg ccagatggac cacctgatcc agggcgtggc ccccgcgggg
     1801 gaactggacc ggggcaaggt gcggcaggcc caacgggaag tgggccgcaa gctggggaac
     1861 atcccccgcg cggccgaacg gccccgggtg gaactgcggg aactgaagcc gggcgacgcg
     1921 gtccatttca agaacggcca ttcccgggcc aaggtgctgg aagccgacga cgacaagcaa
     1981 acggcggtca tcctcttgga gggaggcttg aaactttcct gccaatacgc cgacctgggc
     2041 cccctgggcc cggccccggc ccgcgcgccc ctccggcccc agcccacggc cctggaaaag
     2101 gccaaggcgg acaagggcgc cctggaactg gacctgaggg gccggctggt ggaccaggcc
     2161 ctgcccctgc tcgataaatt catcgacgac gcgctcttgg tggacctgcc ctttgtgcgt
     2221 atcatccatg gcaagggaac gggggccctg aaagaggcca tccacaagca cctgccggtc
     2281 cagcaccccc aggtggagtt ttccttggct gaacccgccc aaggcggcgc cggcgtgacg
     2341 gtggtgaggt tcaagaaatg aaggatcttc attaccacca aggcaccaag acaccaaggg
     2401 aaccctggaa cggggtcccg gtctttgaac cctctttctg cctttcttgg tgtcttggtg
     2461 ccttggtggt gaagagaatg atgccgaggt tttcgtgaaa tattctgacg acatcatcca
     2521 gcgcgtgcgg gacgccgcgg acatcgtgtc cgtggtgggc gagtatgtgc ccttgaagaa
     2581 ggccggttcc agctacaagg ggctttgccc cttccacagc gagaagtcgc ccagcttcat
     2641 cgtgtcgcct tcccggcaca gcttccactg cttcggctgc ggcaagggcg gcaacgtcat
     2701 caccttcgtg atggaaatgg agaagatgcc cttcccggag gcgctgcgcc acttggcggc
     2761 caaggcgggg gtgccgctcc ccaagcccga ggagacgagg cgggacgagg cggccgaccg
     2821 gcgccgcgag cgcatgctgg ccctgaacga gctggcgggg cggctgtacc acgaaaagct
     2881 gctgtccccc gagggccagg cggcccggga ctattacaag ggccggggtt tcaccaagga
     2941 aatggcggtc aaattccaga tcggctatgc cccccccggc tgggatttcc tgaaaaacgc
     3001 ggcccaaaag gccggtttta gccgggag
//
LOCUS       DASTJJ010000084         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1602161, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000084 DASTJJ010000000
VERSION     DASTJJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602161"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..>1107)
                     /locus_tag="VFR02_01045"
     CDS             complement(132..>1107)
                     /locus_tag="VFR02_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869069.1"
                     /translation="VDAGHAGRSLGATPFVALTAGLLLEALWRRAKGKLSLVFGAALL
                     AFIGWQNWHDYFKVQTRDPACRNDCSWAESRVGELVARDGGATEFFLTSRFEGHPTVL
                     YLAGGKASQCQPLDPSALPAPQDRRPGGAFCFLLGGDAGGMLEYLKACYPGGEVGSRE
                     NPLGETALIGYRVGPQALAKLGKARPGSGRGLLGSYAHLDASGEKPFLMRLDPVLNFG
                     FRDLPGTGSALFIHWRGTFHAARAGDYEWVAVTAGYGRARLQVDGKSVDFTEDPRLSV
                     ELAPGPHRLDLWYRQPATPLSELHLLWRPPETGGLVFVPNGVWEKVTE"
BASE COUNT          222 a          381 c          348 g          156 t
ORIGIN      
        1 gaataaaaag gacaatggtg aaaaagacca agaaaactgc tcgcgaataa agaaatcggg
       61 ggttaaaaaa catggtttct tattttacca ctggaagccc cggagccaag ggcccagagg
      121 cccccagcgg cttattccgt aaccttttcc caaacaccgt tgggcacaaa aacgagcccc
      181 cctgtctcgg gcgggcgcca gagcaggtgc agttcggaga gcggcgtggc gggttggcgg
      241 taccagaggt ccaaccggtg cggacccggg gccaattcca cagacagacg cggatcctcc
      301 gtgaagtcca ccgacttgcc atccacctgt aaacgcgccc gtccatagcc cgccgtgacc
      361 gccacccact catagtcccc cgcgcgggcc gcgtggaagg tgcccctcca atggatgaag
      421 agcgcgctgc ccgtgcccgg caggtcccgg aaaccgaagt tgagcaccgg atccaggcgc
      481 atcaggaagg gtttttcgcc ggacgcgtct aagtgggcat agctccccaa cagcccccgg
      541 cccgagcccg ggcgggcctt gcccagcttc gccagggcct ggggccccac ccggtagccg
      601 atgagggccg tttcccccag gggattttcc cggctgccca cttccccgcc cggataacag
      661 gccttcaggt attccagcat cccgcccgcg tcgcccccca acaggaaaca aaaggccccg
      721 cccgggcggc ggtcctgggg ggccggcagg gccgagggat cgagcggctg gcattgggaa
      781 gccttgccgc ccgccaggta aaggacggtc gggtggccct cgaagcgcga ggtcaaaaag
      841 aactcggtgg cgccgccgtc ccgcgccacc agttcgccca cccggctttc ggcccaggaa
      901 caatcgttgc ggcaggccgg atcgcgcgtt tggaccttga agtaatcatg ccagttctgc
      961 cagccgatga aggcgagcag cgcggccccg aagaccaggg acagtttccc cttggcccgg
     1021 cgccaaagtg cctcgagcaa aagccccgcc gtgagggcca cgaagggcgt ggcgcccagg
     1081 ctgcgacccg cgtggcccgc gtccacc
//
LOCUS       DASTJJ010000085         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_135457, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000085 DASTJJ010000000
VERSION     DASTJJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135457"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFR02_01050"
     CDS             <1..430
                     /locus_tag="VFR02_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9869070.1"
                     /translation="DAEDALQEIFLKVFDRIGKYRGDAAFSTWLYRMTANHCLDILRR
                     RKILSFIGMDRAPEAFDRSAEGRPGAGLGLSPVVERALAKLPEKQRRCLVMREMEELS
                     YEEIARALGLTLGSVKSNIHRAKAFLRERLEKEGVSPDDV"
     gene            454..903
                     /locus_tag="VFR02_01055"
     CDS             454..903
                     /locus_tag="VFR02_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869071.1"
                     /translation="MAKWTDAQVGRALKTLAREPNVLPWSERVWDRIESRLARRRPAA
                     AWRPWSLPARLGLAGAGFAAVLCGLLVYQNSAGQAELASYLLKASDERIAVPEDLGFV
                     KIPVLLSAPSSQALQRAAVPVLLGDEEPAGLEDAGFSGGEDDILSPI"
     gene            961..>1258
                     /locus_tag="VFR02_01060"
     CDS             961..>1258
                     /locus_tag="VFR02_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869072.1"
                     /translation="MMKDGQVVPTTALRAALLLLAVSLAAPLFASDEAPAPLPTKTAH
                     RPQLRQILKQLGLSPAQRHQVAECRASYRKKMAELNGQLKVKQVELENELDKTDP"
BASE COUNT          201 a          403 c          463 g          191 t
ORIGIN      
        1 ggacgccgag gacgcgctcc aggagatctt cctcaaggtc ttcgaccgga tcggcaaata
       61 ccggggggac gcggccttca gcacctggct ttaccggatg acggccaacc actgcctgga
      121 catcctgcgg cgccggaaga tcctgtcctt catcggcatg gaccgggcgc ccgaggcctt
      181 cgaccggtcc gccgaagggc ggcccggggc ggggctgggc ctgagcccgg tggtggaacg
      241 ggcgctggcc aagttgcccg agaagcagcg ccgctgcctg gtcatgcggg agatggaaga
      301 actcagttat gaggagatcg cccgggccct ggggttgacc ctgggatcgg tgaaatcgaa
      361 catccaccgc gccaaggcgt tcctgcggga gcggttggaa aaagagggag tgagccccga
      421 cgacgtttga ggggcttgga aaaggggggt aggatggcga aatggaccga cgcgcaagtg
      481 ggccgggcgc tcaagaccct ggcccgtgaa cccaacgtcc tgccctggtc cgaacgggtg
      541 tgggaccgca tcgagtcgcg cctggcccgg cgccggccgg ccgccgcgtg gcggccttgg
      601 agcctccccg cgcgtttggg gctggcgggc gccgggttcg cggccgtgct gtgcgggctc
      661 ttggtctacc aaaactcggc gggccaggcc gagctggcgt cctacctgtt gaaggcctcg
      721 gacgagcgga tcgccgttcc cgaggacctg gggttcgtca agatcccggt gctcctgtcc
      781 gcgccctcgt cccaggcgct gcaacgggcc gccgtgccgg tgttgttggg ggacgaggag
      841 ccggcggggc ttgaggacgc cgggttttcc ggcggtgagg atgatatcct gagcccgatc
      901 tgatccggcg ggcggtcaag gcggggcccg aagggccggt cccgttgaaa ggggagttcc
      961 atgatgaagg acggccaggt cgttcccact accgcgctcc gggcggcgct gctcttgttg
     1021 gcggtgtccc tggcggcccc cctgttcgcc tccgacgagg ccccggcccc cctgcccacc
     1081 aagacggccc accgcccgca actgcggcag atcctgaaac agttgggcct cagccccgcc
     1141 cagcgccacc aggtcgccga gtgccgcgct tcctaccgca aaaagatggc cgagctcaac
     1201 gggcagctga aggtgaagca ggtggagctg gagaacgaat tggacaagac cgatccgg
//
LOCUS       DASTJJ010000086         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_135463, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000086 DASTJJ010000000
VERSION     DASTJJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135463"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFR02_01065"
     CDS             <1..212
                     /locus_tag="VFR02_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HET9869073.1"
                     /translation="TAMVPAFEILVNSLAMANTIRDGDNHKIPSLITTGLAQGMQSME
                     VSLRGLVAAKKITQAQMDEILAEES"
     gene            212..1291
                     /locus_tag="VFR02_01070"
     CDS             212..1291
                     /locus_tag="VFR02_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9869074.1"
                     /translation="MATIDEMFKTMVERKASDMHLTIGNPPLIRAKGELVPLADWVLT
                     EQVMHVLIYETMTPEQWELSWARHDYDYAYEVPGLARFRANVFFNQQGMAAVFRTIPA
                     KILTLDDLGMPPVLKQLAKIKRGLILVTGPTGSGKSTTLAAVIDDINSNREANILTIE
                     DPIEFVHPVKKCKITQREVHRDTHSFKAALRSSMRQDPDIILVGEMRDYETISLAIAA
                     AEMGVLVFGTLHTNSAAKTIDRIINVFPPDERDKARSALAASTKGIVAQQLCPTKDGK
                     GRCAAVEILNYDKSLSNLIRTGKVSGIQALIEMSAKNGMQTMDQCLMKYIEEGKITAE
                     QAYLKANDKKKFQALMEKEAAEAPQ"
     gene            1339..1599
                     /locus_tag="VFR02_01075"
     CDS             1339..1599
                     /locus_tag="VFR02_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET9869075.1"
                     /translation="MPHYDYQCADCGRFELWQSIRDRALEACPTCGAKVERLISAGIG
                     FVLKGSGFYQNDSKSAPVPDPPPAAGPGCGACGGPDPCPNGN"
     gene            1653..1835
                     /locus_tag="VFR02_01080"
     CDS             1653..1835
                     /locus_tag="VFR02_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869076.1"
                     /translation="MAPGWILGIVAGYVLFNVGLWKSDSTPKPQDTYWALAMLAGTLL
                     MFLSVLLWAVPTFFNS"
     gene            1847..2185
                     /locus_tag="VFR02_01085"
     CDS             1847..2185
                     /locus_tag="VFR02_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869077.1"
                     /translation="MQELVTGSQAQQIVLLLCLLLTLGSAAFGFFWGRRLPKPQRPLL
                     WAQAGLAALGGPLVAALWFVYNAIEDHYGLDSVKALGLNAALFLAVGVLFSYLFFWVI
                     PGRFRKTRKA"
     gene            2221..3084
                     /locus_tag="VFR02_01090"
     CDS             2221..3084
                     /locus_tag="VFR02_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9869078.1"
                     /translation="MDVLKEVHRLEVVTRGAVDTLLRGAYQSNFRGQGLEFQQVREYH
                     PEDDHRAIDWNVTARLNSPFVKTFTEERELQLILAVDLSASLWVGTGPVSKRETALRL
                     SALLAFAAVQHQDRVGLLLFTDRVERYLPPRRGRTGAFRVLRELVGFKPQGRGTDFAP
                     AFRLLNAALKRRSVVVLASDFTVALPPSEAGVLARRHDLVAAVLEDPLEKGFRLPARV
                     AVRDPETGRVGVLRPGSVREAREADRFRESQLKLLVRQGADQLFLQAGQNVAPALMRF
                     FRRRLERQGRR"
     gene            3086..>3835
                     /locus_tag="VFR02_01095"
     CDS             3086..>3835
                     /locus_tag="VFR02_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9869079.1"
                     /translation="MDGGTLHFAHPDWLWALAALPLAPLSFWLAARSRRSTLALGSAP
                     LVAAAPGALSAFALPWTLRLLALGLCLVAAARPQSGRQKVMEKKPVTDLFVAFDVSSS
                     MMLQDLKPDRVEAAKKFLGEFLDEVKDVQVGIEVFARLPFTQCPMTTDTQVVKALLQN
                     VEIGSVKVDGTAIGDALVACLNRLGKGSGDTGSGAGGSLMAKVAGDVPVPGPNDHQAI
                     ILLTDGGDNASKIDHLTAAKLAARQGVKIYAI"
BASE COUNT          659 a         1324 c         1222 g          630 t
ORIGIN      
        1 gcacggccat ggtgccggcc ttcgagatcc tggtgaacag cctggccatg gccaacacca
       61 tccgggacgg cgacaaccac aagatcccct ccctgatcac cacgggcctc gcccagggca
      121 tgcagtccat ggaggtctcc ctgcggggcc tggtggcggc caaaaagatc acccaggccc
      181 agatggacga gatcctggcg gaggagtcct gatggccacc atcgacgaga tgttcaagac
      241 catggtggaa cgcaaggctt ccgacatgca cttgaccatc ggcaacccgc ccctcatccg
      301 ggccaagggg gaactggtgc ccctggcgga ctgggtcctg accgaacagg tcatgcacgt
      361 gctcatctac gagaccatga cccccgagca gtgggagctc agctgggccc ggcacgatta
      421 cgactacgcc tacgaggtgc cggggctggc ccgcttccgg gccaacgttt tcttcaacca
      481 gcagggcatg gcggcggtct tccgcaccat cccggccaag atcctgaccc tggacgacct
      541 gggcatgccc ccggtgttga aacagttggc caagatcaag cggggcctca tcctggtgac
      601 gggcccgacc ggcagcggca aatccaccac cctggcggcc gtcatcgacg acatcaacag
      661 caaccgggaa gccaacatcc tcaccatcga ggaccccatc gagttcgtgc atccggtcaa
      721 gaaatgcaag atcacccagc gggaggtgca ccgggacacc cactcgttca aggcggccct
      781 gcggtcctcc atgcgccagg acccggacat catcctggtg ggtgaaatgc gcgactatga
      841 gaccatctcg ctggccatcg cggccgccga gatgggggtg ctggtcttcg gcacgctcca
      901 caccaactcg gcggccaaga ccatcgaccg catcatcaac gtcttcccgc cggacgagcg
      961 ggacaaggcc cgcagcgccc tggcggccag caccaagggc attgtggccc aacagttgtg
     1021 ccccaccaag gacggcaagg gccgttgcgc ggcggtcgag atcctcaact acgacaagag
     1081 cctctccaac ctcatccgca ccggcaaggt gtccggcatc caagcgctca tcgagatgag
     1141 cgccaagaac ggcatgcaga ccatggacca gtgcctgatg aagtacatcg aggagggcaa
     1201 gatcaccgcc gaacaggcct acctgaaggc caacgacaag aaaaagttcc aggccctgat
     1261 ggaaaaagag gccgccgagg cgccccaata ggtgtcctgg cgttgacacc ccggggggcc
     1321 aaccctatga ttggggccat gccccattac gattatcaat gcgccgattg cggccggttc
     1381 gagctctggc agtccatccg ggaccgggcc ctggaagcct gccccacctg cggggccaag
     1441 gtggaacgcc tcatctcggc gggcatcggt ttcgtcctca aggggagcgg tttctaccag
     1501 aacgattcca agagcgcccc ggtcccggat cccccgcccg ccgcggggcc gggttgcggg
     1561 gcctgcggcg gcccggaccc ctgcccgaac ggcaactgaa gcccaaaggg gcgttgggtc
     1621 cgcaacgatc ttaaatgcga agcgagggtg acatggctcc tggatggatc ttaggcatcg
     1681 tggcgggtta tgttcttttc aacgtgggcc tgtggaagtc cgattccacg cccaagcccc
     1741 aggataccta ctgggccctg gccatgctgg cgggcaccct gctgatgttc ctctcggtcc
     1801 tgctttgggc cgtcccgacc ttcttcaact cctgatccgg gaccccatgc aggaactcgt
     1861 caccggatcc caggcccaac agatcgtcct cctgctgtgc ctgctcctca ccctgggcag
     1921 cgcggctttc gggttcttct ggggccgccg gttgcccaag ccccaacggc ccctcttgtg
     1981 ggcccaggcc ggcctggcgg cgctgggcgg gcccctggtt gcggccttgt ggttcgttta
     2041 caacgccatc gaggaccatt atggtctgga ctcggtcaag gccctcggcc tcaacgccgc
     2101 cctcttcctg gccgtgggcg tccttttttc ctacctcttt ttttgggtga tccccggccg
     2161 attccgtaaa acccgcaaag cctgaccgtc gtccaaaccc ccatggaaaa acgggatccg
     2221 atggacgtcc tgaaggaagt gcaccgcctg gaggtcgtta cccggggcgc ggtggacacc
     2281 ttgttgaggg gcgcctacca aagcaacttc cggggccagg gcctggaatt ccagcaggtc
     2341 cgggaatacc acccggagga cgaccaccgc gccattgact ggaacgtcac cgcccgcctc
     2401 aacagcccct tcgtcaagac cttcaccgag gaacgcgagt tgcagctcat cctggccgtc
     2461 gacctgtcgg cgtccctctg ggtggggacg ggcccggtga gcaaacggga gaccgccctg
     2521 cgcctcagcg cccttttggc ctttgcggcg gtccagcacc aggaccgggt ggggttgctg
     2581 ctgttcaccg accgggtgga acgctacctg ccgccccgcc ggggacggac gggggccttc
     2641 cgcgtgctgc gtgagttggt gggcttcaaa ccccagggac gcgggaccga cttcgcgccc
     2701 gccttccggc tcttgaacgc cgcgctcaaa cgccgcagcg tggtggtgct ggcctcggat
     2761 ttcaccgtcg cgctcccgcc ctccgaggcg ggcgtgctgg cccgccggca tgacctggtg
     2821 gcggcggtcc tggaggaccc tctggaaaag ggcttccggc tgcccgcccg ggtggcggtg
     2881 cgggaccccg agacgggccg ggtgggcgtg ctccggcccg ggtccgtccg cgaggcacgc
     2941 gaagcggacc ggttccggga atcccagctc aagctcctgg tccgccaggg cgcggaccaa
     3001 cttttcctcc aggccggaca gaacgtcgcg ccggccctga tgcggttctt ccgccggcgc
     3061 ttggaacgac agggcaggcg ctgacgtgga cggcggcacc ctccatttcg cccatccgga
     3121 ctggctttgg gccctggcgg cgctgcccct ggcgccgctg tccttctggc tcgcggcccg
     3181 ctcccgccgg tccacgctgg ccttgggaag cgcccccctg gtggcggccg cccccggggc
     3241 cctttcggcc ttcgccctgc cctggaccct gcgcctgttg gccctgggcc tctgcctggt
     3301 ggccgcggcc cggccccagt cgggccggca gaaggtcatg gaaaaaaagc ccgtcaccga
     3361 ccttttcgtg gccttcgacg tttccagttc catgatgctg caggacctga aacccgaccg
     3421 ggtggaagcc gccaaaaaat tcctgggcga gttcctggac gaggtgaagg atgtccaggt
     3481 cggcatcgag gtcttcgccc ggttgccctt cacccaatgc cccatgacca ccgacaccca
     3541 ggtggtgaag gccctgctcc agaacgtgga gatcggttcg gtcaaggtgg acggcacggc
     3601 catcggggac gccctggtgg cctgcctgaa ccgcctgggg aagggatcgg gggacacggg
     3661 atccggggcc ggcgggtcct tgatggccaa ggtggcgggg gatgtcccgg tccccgggcc
     3721 gaacgaccac caggccatca ttctcttgac cgacggcggc gacaacgcca gcaagatcga
     3781 tcacctgacc gcggccaaat tggcggcgcg ccagggggtg aagatctacg ccatc
//
LOCUS       DASTJJ010000087         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1358056, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000087 DASTJJ010000000
VERSION     DASTJJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358056"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..>1608
                     /locus_tag="VFR02_01100"
     CDS             263..>1608
                     /locus_tag="VFR02_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9869080.1"
                     /translation="MTESSFKQSFKIYMRLLDYAGEYRWRIWLTWLFMIVTALSTSFV
                     FLQLKPIFDGAFLVTPDPQAQFDHLAYFIIPWTLCAAVLRSGSGYGQAYFMGFLGQQI
                     VQKLRNNLYDHFLKLPMAFFNAQRTGNISNRITNDVQVLQDSIPNVVGSGVSSVLMVL
                     SLSVSLIYFEWKMALMALVVFPLGVLPIYHFGRKIRRASRERQELLGTLNSQIFETLA
                     GIRVVKGFGMESFEKNKFHRTNRGYFEVSMRSVRAFAASSPIVEAMTTLVMLGLVVWL
                     AHESLFPPRDITGGDFVRFFAITATLYPHLKNFNGLWGGMQNALGAAERCFEILDTPD
                     SMMDAADAVQVGPLKKALEFKGVSFEYLPGHPVLRDVSFAVKKGETVALVGPSGGGKS
                     TLADLIPRFYQPTSGRILWDGVDLARIKLASLRSHIGLVTQETILFHDSILNNIAYG"
BASE COUNT          304 a          454 c          475 g          375 t
ORIGIN      
        1 ggcctgccgc gcaaggccgg catcttcaag ggccgggaga tggccttccg ctggctgacg
       61 ctggaagaac ccaccacgtc ccagggggaa gccctgcggg aagcgggcgt ggaacccttc
      121 ctcttttgac ccgataaaca agggaacaag ccgcgcgggg ccgtatttca acgcttttcc
      181 gccccggagg accgaaaaag cgtgccgggg ccgggggctt cgctgttaaa atgacaggcc
      241 tttcaacact acgggggccg tcatgacgga aagctcgttc aaacaatcct tcaagatata
      301 tatgcggctg ttggattacg ccggggaata ccggtggcgc atttggttga cttggttgtt
      361 catgatcgtg acggccctct ccaccagttt tgtcttcctc caattgaagc cgatcttcga
      421 tggggctttc ctggtgaccc cggatccaca ggcgcagttc gaccacttgg cttatttcat
      481 aatcccctgg acattgtgcg cagccgtcct gcgttcgggt tcgggttatg gccaggctta
      541 tttcatgggt ttcctgggac aacagatcgt tcaaaagttg cggaacaacc tttacgatca
      601 tttcctgaag ctgcccatgg cctttttcaa tgcccagcgc acggggaaca tttccaacag
      661 gattaccaac gatgtgcagg tgctccagga ttccatcccc aacgtggtgg ggagcggggt
      721 ttcttcggtc ctcatggtcc tctccctgtc cgtgtccctc atttattttg agtggaagat
      781 ggccttgatg gccctggtgg tctttccttt gggggtgctt cccatctacc acttcgggcg
      841 gaagatccgc cgggcctcgc gggaaaggca ggaactgttg gggacgttga actcccagat
      901 attcgagacc ctggcgggca tccgggtggt caaggggttc gggatggaat ccttcgagaa
      961 aaacaagttc cacaggacca atcggggcta ttttgaggtg tccatgcgtt ccgtccgggc
     1021 ctttgcggcc tcctccccca tcgtggaagc aatgaccacc ttggtgatgc tggggctggt
     1081 agtttggttg gcccatgagt cgcttttccc gccgcgggac atcaccggcg gcgatttcgt
     1141 ccgtttcttc gccattacgg ccacacttta ccctcactta aagaatttca acgggctctg
     1201 gggcgggatg caaaatgcct tgggcgccgc cgagcgttgt ttcgagatat tggacacgcc
     1261 cgacagcatg atggacgcgg cggatgcggt ccaagtgggc ccgctgaaaa aggcgctgga
     1321 gttcaagggc gtgagcttcg agtacctgcc gggccatccg gtattgcggg acgtctcctt
     1381 tgccgtgaag aaaggcgaaa cggtggctct cgtgggtccc tcgggcggcg gcaagagcac
     1441 attggcggac ctgatccccc gattttatca gcccacttca gggcgcatcc tttgggatgg
     1501 agtggacttg gcccggataa aactcgcttc cctgcgttcc cacatcggat tggtgaccca
     1561 agaaacgatc ctgttccacg attccatcct caacaacatc gcttacgg
//
LOCUS       DASTJJ010000088         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_991483, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000088 DASTJJ010000000
VERSION     DASTJJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991483"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1215
                     /locus_tag="VFR02_01105"
     rRNA            <1..>1215
                     /locus_tag="VFR02_01105"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          284 a          314 c          435 g          182 t
ORIGIN      
        1 agaagtgaga atgccggtat gagtagcgaa agacgggtga gaatccgtgc cgccgtaagc
       61 cagaggtttc ctgggcaagg atcgtccacc cagggtgagt cgggacctaa gccgaggccg
      121 aagggcgtag gcgatggaca accggtggat attccggtac cacaaccggc cgttatcagc
      181 gaagcgggga cgcaggagga taggcgcagc gggcgatcgg tcgtgcccgt ccaagccggc
      241 aaggggcctt ggtaggtaag tccgccaggg aataccccaa gcggtgatgg cgagggaaat
      301 tcagtactga agtggctgaa tccacactgc cgggaaaagc cgctagcgag gacggttgtg
      361 cccgtactga aaccgacaca ggtaggcggg gcgagaagcc caaggcgcgc gggagaaccc
      421 ttgctaagga actcggcaaa atccccccgt aacttcggga gaaggggggc cccggtagcg
      481 tgcgcaagtg cgcgaggggg ccgcagagaa acaacccaag cgactgttta ccaaaaacac
      541 aggtctctgc gaacgcgcaa gcggaagtat aggggctgac acctgcccgg tgctggacgg
      601 ttaaggggag gcgtgagagc gccgaaccga agccccagtg aacggcggcc gtaactataa
      661 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggtgtaac
      721 gacttgggtg ctgtctcggc agggggcccg gtgaaattga aggcctcgtg aagatgcgag
      781 gtacccgcga ctggacggaa agaccccgtg gagcttgact gtaccctggc attggacctt
      841 ggtcgtttct gtagaggata ggtgggaggc agcgaacccg ggccgccagg ctcgggggag
      901 ccgacggtgg aataccaccc ttgaacgact ggggttctaa ccgactcccc tgaagcgggg
      961 agcgggaccg tgccaggcgg gcagtttgac tggggcggtc gcctcccaaa tggtaacgga
     1021 ggcgcccaat ggttccctca gcacggttgg acatcgtgcg tcgagtgcaa aggcacaagg
     1081 gagcttgact gcgagtggga cgccacgagc agggccgaaa ggcgggctta gtgacccact
     1141 ggcgctgagt ggaagggcca gtgatcaacg gataaaagct accccgggga taacaggctg
     1201 atcttgcccg agagt
//
LOCUS       DASTJJ010000089         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_746562, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000089 DASTJJ010000000
VERSION     DASTJJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746562"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFR02_01110"
     CDS             complement(<1..507)
                     /locus_tag="VFR02_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869081.1"
                     /translation="MPKSAKLSTEEAKAKALEQFALGATVRDAMKAVGRQPKTHENWR
                     ASDLDYKNAVDAARDRSTRAKDAGKDPALYELGFAEWRKRFLGRDTYPHQQMWIDVLE
                     EREPVVFHPAITYRPGNHNYVLINTPPAHAKSSTITMDYATYKLCMNPAYRVIIISKI
                     AEMASKFLH"
     gene            complement(716..901)
                     /locus_tag="VFR02_01115"
     CDS             complement(716..901)
                     /locus_tag="VFR02_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869082.1"
                     /translation="MTFWDDLGDRIADDDEFARLFAEPRVIVVSAEGYDQMMGRLDEP
                     PRVLPGLRRLMEQAREG"
BASE COUNT          463 a          853 c          704 g          403 t
ORIGIN      
        1 gtggaggaac ttcgacgcca tctcagcaat cttcgagatg atgatgaccc ggtacgccgg
       61 gttcatgcac agcttgtacg tggcgtagtc catcgtgatg gtggacgact tcgcgtgggc
      121 cggaggtgtg ttgatcagga cgtagttgtg gttacccggc ctgtaggtga tggccgggtg
      181 gaacaccacg ggctcccgct cctccagcac gtcaatccac atttgctggt gcgggtacgt
      241 atccctcccg aggaaccgct tgcgccactc cgcgaacccc agctcataca gggcggggtc
      301 cttgcccgca tccttcgccc tggtgctacg gtcacgggcc gcgtccaccg cgttcttgta
      361 gtcaaggtcc gatgcccgcc agttctcgtg cgttttgggc tgccggccga cagccttcat
      421 cgcatcccgc accgtggcgc ctagcgcgaa ctgctccagg gccttggcct tggcctcctc
      481 ggtggagagc ttcgcggact tgggcaaggt gagccttcca atagggaagg cccccgccga
      541 agcgagggcc tacatgtctt cgagttgaga gcgaatctcc cggacggcga gcctgcggtc
      601 gatccggtgc gaacggcgct tcactgccct ggtgacgccg ggtctgctaa gcgacggcag
      661 gtacttgcgg caccatcggg agaaggcgtc ggactcatcc caggtgcgcg ggtgctcacc
      721 cctcacgggc ctgctccatc agccggcgca agcccggcaa cacgcggggc ggctcatcga
      781 gccgacccat catctggtcg tacccctcag ccgacacgac gatcacccgc ggctccgcaa
      841 agaggcgggc gaactcgtcg tcatccgcga tcctgtcacc caggtcatcc cagaacgtca
      901 tgcgtcagac tcctccaggg cgaacccctg catcaccagc ttcgggccat gctcccgggc
      961 gtggtgccca cacagcgtga gcaccgccaa cccccgggtc acccacaccg tcaccgcggc
     1021 aaccgcaggg ccacaccggt cacacgtccc agccaccacc gggcgctgat ccgccacagc
     1081 cgccgccact gctggcgcct gccccatcgc cgtgagcgtg tcctggcgca cgtcgaacgc
     1141 acaccgcggg cagtagatgc cctgcgacgc ccaccgcgtc ttgcagcgga agcaatacct
     1201 cggagccatc acggccctcc tactggcaga tcgggcaccc cattgaccaa caagcctgcg
     1261 agtgaccaca ccccttgccc tcccactcgg ggagagaccc accccaccgc ttcgtgtgag
     1321 caatggcctg catcgcctta cggttctgca cccgcatgtg agacgggtcc acacaccgat
     1381 ggttgccaca cttgcgctgc aacaccttcg tcaacgggaa gtagtccagc ttcccgaacc
     1441 cacccatcaa gtccatccac accttctgag cgggcggata cccagggcca gtccactccc
     1501 agcaaggccc agaagaatcg acgtgagcca tccagtcgaa atcaaactca gccacgccgg
     1561 cacccctcat tcatgtgatt agcctggacg ccggaaaggt tcccagcact cctactatat
     1621 cacatcgagc caagctcgat gctctgcccg gaccgggtgc ggagccctcg cggagcagcc
     1681 gggacggctg gggggataga gggggtacca ggggtacccc tttggggggc gtctgacggg
     1741 cgcgtcagcg ccctctcgcc cggaccggaa ggggtccccc gggaagggac agaacccacc
     1801 cccagaaggt aagaacctga ccagccccga gggggcctcc ccggctcccg aggggtcgcc
     1861 cctccggccc caaccaaccc aaccacaagc tcgggtaacc cctcgcccca aaccttcctg
     1921 ttcactaatt tactacgttc atcggtatct ggacccaccc acacaatccc caaaaaaact
     1981 tcaggcccca cagggcctgc aagcccctga cctgcgaaga taccgggaaa atatttctct
     2041 cccgagagta cacatggggg agggaggtcg agttaagcat cccccgggtc aagctcagct
     2101 cacacccccg agggctggat gggacccgct ccgaggggtg ccacgcaccc cgatggtgcc
     2161 cccaatgggg cacgagtgga gggcataggt gggcatatgt gacagcgcac atcactccat
     2221 ccacaacatg ccgctacctg tccagctcgt gccggcatcc ggccggcatc gagaccagga
     2281 gggacgaaca agacatagat ggacgtctat atgaggctga ctctacgcgt gggtatgcgg
     2341 agggcatctc cacttatcgt ggtgacccgt gctccagctc ggtctaggtc acggtctttg
     2401 taacggtttg gtcacggtgg tca
//
LOCUS       DASTJJ010000090         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_502361, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000090 DASTJJ010000000
VERSION     DASTJJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502361"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1390)
                     /locus_tag="VFR02_01120"
     CDS             complement(<1..>1390)
                     /locus_tag="VFR02_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9869083.1"
                     /translation="KLPEFYRIHQAGGALYPPDRLPLARALRGERTIVDDLEIRRPDQ
                     VVPVQAWGIPILDEADRVKYAVGVLLDITERQKSLRALQEREEFFRTLFEESPIGMTL
                     VFPDWRILNVNQALCDLLGYSRSELMAMDAKDCTHPDDLEIDQSLSKKLFEKLIPKYQ
                     IEKRYFTKDGRTIWCKVSASLIRDVDDNPLFRLAMIENITEQKEAELALKESEKRFRR
                     IFEQSAVGMVFASLDGKVMDANQAYCEMLGYPKAELLGRPFSDFTHPQDVRMTDGLAQ
                     SVPGGPTRFRFEKRYVAKDGRVIWASLTTLVVQDSKGQPVHILSMVEDITTRREAEQA
                     LRENQEQTRLILDSAGEGIYGIDPQGHCTFCNPAGLRLLGFRDLAQVLGKNMHYLVHS
                     KRADGSPYPLRDCPVFKSFGAGTECHVTDEFFQRADGTAFPVEYRSHPMQRGGAPLGA
                     VITFMDITERKQV"
BASE COUNT          206 a          428 c          493 g          263 t
ORIGIN      
        1 cacctgcttg cgctcggtga tgtccatgaa ggtgatgacc gcgcccaggg gcgccccgcc
       61 gcgttgcatg ggatgggagc ggtattccac cgggaaggcc gtcccgtcgg cgcgctggaa
      121 gaactcgtcg gtgacgtggc attcggtgcc ggcgccgaag gacttgaaga ccgggcagtc
      181 ccgcagcggg tagggggagc cgtccgccct tttggagtgg accaggtagt gcatgttctt
      241 gcccaatacc tgcgcgaggt cccggaagcc cagcaaccgc agccccgccg ggttgcagaa
      301 ggtgcagtgg ccctgggggt cgatgccgta gatgccctcg ccggccgagt ccaggatcag
      361 ccgggtctgt tcctggttct cccgcagggc ctgctcggct tcccggcggg tggtgatgtc
      421 ctcgaccatg ctcaggatgt ggaccggctg tcccttgctg tcctggacca ccagggtggt
      481 caggctggcc cagatgaccc ggccgtcctt ggccacatag cgtttctcga accggaaccg
      541 cgtggggccg ccggggacgg attgggccag cccgtcggtc atccggacgt cctggggatg
      601 ggtgaaatcc gagaaggggc ggcccagcag ttcggccttg gggtagccca gcatctcgca
      661 ataggcctgg ttggcgtcca tcaccttgcc gtccagggac gcgaagacca tgcccaccgc
      721 gctctgctcg aagatgcgcc ggaagcgttt ctcgctttcc ttcagggcca gttcggcctc
      781 cttctgctcg gtgatgttct cgatcatggc caggcggaag agggggttgt cgtccacgtc
      841 ccggatgagg gaggccgaca ccttgcacca gatggtgcgg ccgtccttgg tgaaatagcg
      901 tttctcgatc tggtacttgg ggatcagctt ctcgaagagc ttcttggaca gggactggtc
      961 gatctccagg tcgtccgggt gggtgcagtc cttggcgtcc atggccatga gctcgctgcg
     1021 gctgtagccc agcaggtcgc acagggcctg gttcacgttg aggatgcgcc agtcggggaa
     1081 gaccagggtc atgccgatgg ggctttcctc gaacagggtg cggaagaatt cctcgcgttc
     1141 ctggagggcg cgcaggctct tttggcgctc ggtgatgtcc agcagcacgc ccacggcgta
     1201 cttgacccgg tccgcctcgt ccaaaatggg gatgccccag gcctgcacgg gcacgacctg
     1261 gtcggggcgc cggatctcca ggtcgtccac gatcgtccgc tcgccccgaa gggcccgggc
     1321 caagggcagc cggtccgggg ggtagagggc gccgccggcc tggtggatcc ggtagaactc
     1381 gggcagcttt
//
LOCUS       DASTJJ010000091         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1235868, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000091 DASTJJ010000000
VERSION     DASTJJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235868"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFR02_01125"
     CDS             <1..500
                     /locus_tag="VFR02_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9869084.1"
                     /translation="SIYVADTGKHRIVKFDPKGNQVAVWGQKGDKVGEFNEPIGLAED
                     STGDIYVADRLNFRIQVFDPNGIPVRHWKVDGWSKDQVNMEPHLAIDKGRGLLYATDG
                     RASRILCYKLDGTELPAIAKDPAGNPLVQVPIGVAVGATGDLYVTDAQAGRVLKLAGE
                     AKTNP"
     gene            521..1513
                     /locus_tag="VFR02_01130"
     CDS             521..1513
                     /locus_tag="VFR02_01130"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9869085.1"
                     /translation="MAAKKPKRKKAGTRRGGRGLKVLLVFLLMALSSAVIYVLVGQLH
                     KALVQAKTREVTPDLVLGGLGNGPGQFQQPWDVAVGPQGDVVATDFGNHRLLKFDPNG
                     KLIFSIGKEGKGPGEFEQPSGVYVDPEGKIYVCDTFNYRIQKFDAQGRFLKSWEHAFY
                     GPKGIMGNGHGVIYVVDTGNHKIQAFSENGDFLREWGGYGPEAGKLKEPIGGVVGPEG
                     SVYVADTDNLRVQKFDPNGKPLGSFKIPTWRGKNEETPYLAFGAGSLWVTNTSEGAVL
                     KYSPSGQLQAVYKLKGTGFSNPAGVAVSPDGQVYVTERNTGKIVRFQPPAPPSR"
     gene            1524..>1968
                     /locus_tag="VFR02_01135"
     CDS             1524..>1968
                     /locus_tag="VFR02_01135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9869086.1"
                     /translation="MKKLSTPKVLIVVPTYNERKNLPALYARIRRHAGRAHLLVVDDG
                     SPDGTGELADSMAAKDPGVRVLHRGRKMGLGTAYVAGFRYALRHRYDLVLAMDADLSH
                     DPANIPRMIGLMKDRDLVIGSRYVKDGGMVNWGLGRFFISKFANSF"
BASE COUNT          380 a          562 c          677 g          349 t
ORIGIN      
        1 gttccatcta cgtggcggat acgggcaagc accgcatcgt caaattcgac cccaagggga
       61 accaggtggc ggtgtggggc caaaagggcg acaaggtggg tgagttcaac gagcccatcg
      121 gcctggccga ggattcgacc ggcgacatct acgtggccga ccgcctgaac ttccgcatcc
      181 aggtcttcga tcccaacggc atcccggtgc ggcattggaa ggtcgacgga tggtcgaagg
      241 accaggtcaa tatggagccg cacctggcga tcgacaaggg ccgtggattg ctctacgcca
      301 cggatgggcg cgcaagccgt atcctctgtt acaagctgga tgggaccgaa ttgccggcca
      361 tcgccaagga ccccgcgggg aacccgctgg tccaggttcc gatcggcgtg gcggtcggcg
      421 ccaccggcga cctttacgtg acggatgccc aggcgggccg ggtgttgaag ctggcgggtg
      481 aggcgaagac caacccctga gggggaccgg aggattccag atggcggcga aaaaaccgaa
      541 acggaaaaaa gcggggaccc gccggggggg acggggcctc aaggtgctgc tggtcttcct
      601 cttgatggcc ctgtcgtccg cggtgatcta tgtgctggtc ggccagctcc acaaggccct
      661 ggtccaggcc aagacccggg aagtgacgcc cgacctggtg ctgggcgggt tgggcaacgg
      721 gccggggcag ttccaacagc cctgggacgt ggcggtgggc ccccaggggg acgtcgtcgc
      781 cacggatttc gggaaccacc ggctgctgaa gttcgatccc aacggtaagt tgatcttttc
      841 catcggcaag gaagggaagg ggcccgggga gttcgagcag cccagcggcg tttatgtgga
      901 tccggaaggc aagatctacg tttgtgacac cttcaattac cggatccaaa aattcgacgc
      961 gcaagggcgc ttccttaaat cctgggagca cgctttttac ggccccaaag ggatcatggg
     1021 caacggccac ggggtgatct acgtagtgga tacgggcaac cataagatcc aggcgttcag
     1081 cgagaacggg gattttctca gggagtgggg cggttacggg ccggaggcgg gcaagctcaa
     1141 ggagcccatc ggcggggtgg tggggcccga ggggtccgtg tatgtggccg acacggacaa
     1201 cctgcgggtc cagaaattcg accccaacgg aaagcccttg ggttccttca agatcccgac
     1261 ttggcggggt aagaacgagg aaacccccta cttggccttc ggggccggtt cgctctgggt
     1321 gaccaatacc agcgaggggg cggtgttgaa atattcgccc tcgggccaat tacaggcggt
     1381 ctacaagttg aaggggaccg gtttttccaa cccggcgggt gtggcggttt cccccgatgg
     1441 gcaggtctac gtgaccgagc ggaatacggg caagatcgtt cggttccaac ctcccgcccc
     1501 gcccagccgg tagggccccc gttttgaaaa aactttccac gccgaaggtc ctgatcgtcg
     1561 tcccgaccta caatgagcgc aagaatctcc cggctcttta tgcccggatc cgccgccatg
     1621 cgggtcgggc ccatctgctg gtggtggatg acggttcccc ggacgggacc ggggaactgg
     1681 cggattcgat ggcggccaag gaccccgggg tgcgggtgct ccaccggggt cgaaagatgg
     1741 gattggggac ggcctatgtg gccggattcc gctatgccct gcggcaccgc tatgacctgg
     1801 tgctggcgat ggacgcggac ctgtcccatg atccggccaa catcccccga atgatcggcc
     1861 tgatgaagga ccgcgacctg gtcatcggct cccgctatgt caaggacggg gggatggtga
     1921 attggggttt gggccgtttt ttcatcagca aattcgccaa cagtttcc
//
LOCUS       DASTJJ010000092         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_991936, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000092 DASTJJ010000000
VERSION     DASTJJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991936"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFR02_01140"
     CDS             <1..338
                     /locus_tag="VFR02_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869087.1"
                     /translation="FGKIMDKAVAQVFKEQMEKGHLEMPAPDLSGEKHIRVSELTPAA
                     QYKDVISQLPQIWEPVHHLTGKHSEVYQASCVEFVLEGLHLAGKLSRQKVGELFDYQP
                     SAAQKRKKE"
     gene            454..>2036
                     /locus_tag="VFR02_01145"
     CDS             454..>2036
                     /locus_tag="VFR02_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869088.1"
                     /translation="MITHDYSEWHEPEKPPFTVEELVEAVKELMMQYHVSFDEALKHL
                     QEKGIPYNQFLKVGGLEELIQGFLDKIELERSKLLDGHRLDGLLTRMEEDFRRRSKEL
                     ERLFDKLQDDKLQKRLDDALQSRDASALYNLDWQLSRAGQRAGDEARRQLQALTQLAG
                     QLRALRALYEEYDFTGEKTPTPAEAGRLREKLEALDELEQRLIDAVEYGDLFNMDLGQ
                     LQEMLGEEASRAFEAKREELRRQFTEALKATGDVDHDEDTDLYKITPGAARKIGEKAL
                     KEIFSRLFSDGLGRHHATFAGEGNVETTAVRPYEFGDSLASLDLPTSLMNSAVRQLGD
                     PGAVPGRIQIDSRDFWVHQTKGAAASAIVMLIDQSGSMARYGRFYNTKKLALALDSLI
                     RTQYPEDKLYFVTFATFARQVPVGAIPELAPKPVTLMGGGVNMRVDFSKGLDERARVM
                     VPEHFTNLQKGLELARVLLANSDTKNKSILLITDGAPTAFYEGPQLYLTYPPNDRTYQ
                     RTLREVRAVTDEGITLNTF"
BASE COUNT          425 a          665 c          619 g          327 t
ORIGIN      
        1 tcttcggcaa gatcatggac aaggcggtgg cccaggtctt caaggaacaa atggagaagg
       61 ggcatttgga aatgcccgcg cccgacctgt cgggcgagaa gcacatccgg gtgtccgagc
      121 tcacccccgc cgcccagtac aaggacgtca tttcccaact gccccagatc tgggaaccgg
      181 tccaccacct gaccgggaag cattcggagg tctaccaggc ctcctgcgtg gagttcgtgc
      241 tggaagggtt gcacctggcg ggcaagctgt cgcgccaaaa ggtgggcgaa ttgttcgact
      301 atcagcccag cgcggcccaa aaacggaaga aggaatgaat cccattcacc gcaaagacgc
      361 caaggcgcaa aggaaagatc ttttggaaaa gaattcattc gggcttccct tggcgccttg
      421 gcgtctttgc ggtgaaatgg ttttgaggtt ttcatgatca cccacgatta ttccgagtgg
      481 cacgaacccg agaagccgcc cttcacggtg gaggaactcg tcgaggccgt caaggaactg
      541 atgatgcagt accacgtgtc cttcgacgag gccctcaagc acctgcagga gaagggcatc
      601 ccctacaacc aattcctgaa ggtgggcggc ctggaggaac tgatccaggg gttcctggac
      661 aagatcgagc tggagcgctc caagctgttg gacggtcacc gcctggacgg gcttttgacc
      721 cggatggagg aggacttccg gcgccgctcc aaggaactgg aaaggctttt cgacaagctt
      781 caggacgaca agcttcaaaa acgcctggac gacgccctcc aaagccgcga tgcctcggcc
      841 ctctacaacc tggactggca gctttcccgg gcgggccaaa gggccggcga cgaggcgcgg
      901 cgccaattgc aggccctcac ccaactagcc gggcaactca gggccctgcg ggccctttac
      961 gaggaatatg acttcacggg cgaaaagacc cccacccccg ccgaggccgg gcgcctgcgg
     1021 gaaaagttgg aggctttgga cgagctcgag caacgcctga tcgacgcggt ggaatacggc
     1081 gacctgttca acatggacct gggccagctc caggagatgt tgggcgagga ggcctcccgg
     1141 gcctttgagg ccaagcgcga ggagctgcgc cgacagttca ccgaggccct gaaggccacc
     1201 ggcgacgtgg accacgacga ggacaccgac ctctacaaga tcacgcccgg cgcggcgcga
     1261 aagatcggtg aaaaggcgtt gaaggagatc ttttcccggc tcttctccga cggcctgggc
     1321 cgccaccacg ccactttcgc gggcgagggc aacgtggaga ccaccgccgt gcgcccctac
     1381 gagttcggcg actccctggc ctcgctggac ctgcccacca gcctgatgaa ctccgccgtg
     1441 cgccagttgg gcgatcccgg ggccgtgccg ggccgtatcc agatcgacag ccgggacttt
     1501 tgggtgcacc agaccaaggg cgcggcggcc tcggccatcg tcatgctcat cgaccaaagc
     1561 ggctccatgg cccgctacgg gcgcttctac aacaccaaaa aactggccct ggcgttggat
     1621 tcgctcatcc gcacccagta ccccgaggac aagctctatt tcgtcacctt cgccaccttc
     1681 gccaggcagg tgcccgtggg cgccatccct gagctggccc ccaagcccgt caccctgatg
     1741 ggcgggggcg tgaacatgcg ggtggacttt tccaaggggc tggacgaacg cgcccgggtc
     1801 atggtgcccg agcacttcac caacctgcaa aaggggctgg agctggcccg ggtgctcttg
     1861 gccaactcgg acaccaagaa caagagcatc ctgctcatca ccgacggcgc gcccacggcc
     1921 ttttacgagg gcccccagct ctacctgacc tacccgccca acgaccgcac ctaccagcgc
     1981 accttgcggg aagtgcgcgc cgtcaccgac gagggcatca ccctcaacac cttcat
//
LOCUS       DASTJJ010000093         1205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_136313, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000093 DASTJJ010000000
VERSION     DASTJJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136313"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFR02_01150"
     CDS             <1..405
                     /locus_tag="VFR02_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9869089.1"
                     /translation="GRRLQLKVSEVMRKGAEIPKVSLSAHFQGIVREINAKKVGATLV
                     LDAKGRLAGIIVDGDLRRALLKKPDVRHWNAANLRSPRPKTVPPGTTLGEALQLMEEN
                     AIFQLIVADDKQRPLGLVHLHDLLGRGQVRLA"
     gene            402..959
                     /locus_tag="VFR02_01155"
     CDS             402..959
                     /locus_tag="VFR02_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET9869090.1"
                     /translation="MKKTRPDPKRIRLFLTDVDGCLTDGKIYVGKGLELMGFDIQDGI
                     GHRLAEVGGLEVGWLSGRHSEATAERAGKLRVAHVYLGVLDKLKKAQAHCGERGLALR
                     QVAYLGDDLIDLPLMAQVGWAVAPVNARPEVKRAAHAVTRAAGGAGAFREAVETLLKA
                     QGRWTEAVRKFHARNAAPPDGLRLD"
     gene            967..>1205
                     /locus_tag="VFR02_01160"
     CDS             967..>1205
                     /locus_tag="VFR02_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HET9869091.1"
                     /translation="MARPLQKIKNDLIYYAVRALLALLRRMPDGVSRRAGRAFGACFF
                     RLAGGERRKTLRNLRTAFPDRDKAALARLAREVWR"
BASE COUNT          195 a          408 c          439 g          163 t
ORIGIN      
        1 gggcggcgcc tgcagctcaa ggtctccgag gtgatgcgca agggcgccga gatccccaag
       61 gtgtccctga gcgcgcattt ccagggcatc gtgcgggaga tcaacgccaa gaaggtgggc
      121 gccaccctgg tgttggacgc caagggccgc ctggccggca tcatcgtgga cggcgacctg
      181 cgccgggccc tgctcaagaa gcccgacgtg cggcattgga acgccgccaa cctgcgcagc
      241 ccccggccca agaccgtccc gcccggcacc accctgggcg aggcgctgca gttgatggaa
      301 gagaacgcca tcttccagct gatcgtggcc gacgacaaac aaagacccct gggcctggtg
      361 cacctgcacg acctgctggg acggggccag gtccgcctgg catgaaaaag acccggcccg
      421 acccgaagcg catccggctt ttcctcaccg acgtggacgg ctgcctgacc gacggcaaga
      481 tctacgtggg caagggcttg gagctgatgg ggttcgacat ccaggacggc atcggccacc
      541 ggctggccga ggtggggggg ctggaagtgg gctggctctc gggacggcat tcggaggcga
      601 ccgccgagcg cgcggggaag ctgcgggtcg cccacgtcta cctgggggtg ctggacaagc
      661 tcaaaaaagc ccaggcccat tgcggggagc ggggcctggc gctgcgccaa gtggcctacc
      721 tgggcgacga cctgatcgac ctgcccctga tggcccaagt gggttgggcc gtggccccgg
      781 tgaacgcccg ccccgaagtg aagcgcgccg cccacgcggt gacccgggcc gccggcgggg
      841 ccggggcctt ccgggaagcg gtggagaccc tgctgaaggc ccaaggccgc tggaccgagg
      901 ccgtccggaa gttccacgcg aggaacgccg ccccgccgga cggcctgcgg ctggactgat
      961 ccttccgtgg cgcgtcccct ccaaaaaata aagaacgacc tcatttatta tgccgtccgc
     1021 gccttgctgg ccctgttgcg ccggatgccg gacggggttt cccgccgggc ggggcgggcc
     1081 ttcggcgcct gcttcttccg gctggccggg ggggagcgcc gcaagaccct gcgcaacctg
     1141 aggaccgcct tcccggaccg ggacaaagcg gccttggcgc ggctggcccg ggaggtctgg
     1201 cggga
//
LOCUS       DASTJJ010000094         1467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_992088, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000094 DASTJJ010000000
VERSION     DASTJJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992088"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /gene="gcvPA"
                     /locus_tag="VFR02_01165"
     CDS             complement(<1..1203)
                     /gene="gcvPA"
                     /locus_tag="VFR02_01165"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9869092.1"
                     /translation="MAYIPITPDDEKEMLQAVGVPKLDDLFADVPPAVLLKKGLDLPA
                     GLSEPELKRWFTLIGKNNLPVSGRPCFLGAGLYYHEVPSAVKAVVTRPEFLTSYTPYQ
                     PEIAQGTLQVLFDFQTYMVELTGLQVSNASLYDGATAVAEATLLALRVKKQASLVFVS
                     RAVHPEYRQTIQTYLNPLGIKIQEVGLKGTETDLEALKASLWPNSVVVVQYPNFFGTI
                     EDLRAFSKACREKDCYLVVAVPEAMNLALLPSPGSLGADVCAGSAQSFGNILSFGGPH
                     AGFLTARMDDIRQMPGRIVGGTTDRNGKEGCVLTFQAREQHIRRERAVSNVCTTQSLM
                     ANFASAWLYFKGGAGAQQAAQDGAATAHLLAGEVAKLPGFKIESARYANELVVRPPSP
                     GFADFLAEW"
BASE COUNT          249 a          438 c          510 g          270 t
ORIGIN      
        1 ccattcggcc aggaaatcgg cgaatccggg cgaggggggc cgcaccacga gctcgttggc
       61 gtaccgggcg ctttcgatct tgaacccggg cagtttggcc acctcgccgg ccagcaagtg
      121 ggccgtggcg gcgccgtcct gggcggcctg ttgggccccc gcgccgccct tgaagtagag
      181 ccaggccgag gcgaagttgg ccatgagcga ctgggtggtg cagacgttgg agaccgcccg
      241 ctcccggcgg atgtgctgtt cgcgcgcctg gaaagtgagc acgcagcctt ccttgccgtt
      301 gcggtccgtg gtcccgccca cgatgcgccc cggcatctgg cggatgtcgt ccatgcgggc
      361 ggtgaggaag cccgcgtggg gcccgccgaa ggagaggatg tttccgaagg actgggcgct
      421 ccccgcgcag acgtccgcgc ccagcgaccc gggcgagggc aagagggcca ggttcatggc
      481 ctcgggcacg gccaccacca ggtaacagtc cttttcccgg caggccttcg agaaggcccg
      541 caggtcctcg atggtgccga agaagttagg atattgcacc accaccaccg agttgggcca
      601 gagggaggcc ttgagggcct ccaggtcggt ctcggtgccc ttcaagccca cttcctggat
      661 cttgatgccc agggggttca ggtaggtctg gatggtctgg cggtattcgg gatgcacggc
      721 gcgggacacg aagaccaggg aggcctgctt cttgacccga agcgccaaga gggtcgcctc
      781 ggccacggcg gtcgcgccgt catagaggga ggcgttggac acctgcaggc ccgtcagctc
      841 caccatgtag gtctggaagt cgaagagcac ctgcagggtg ccttgggcga tctcgggctg
      901 gtagggcgtg taggaggtga ggaactcggg ccgggtgacc acggccttca cggccgacgg
      961 gacctcgtgg taatagagcc cggcgcccag gaagcagggc cgcccggaaa cgggcaggtt
     1021 gttcttgccg atgagggtga accagcgctt gagctcgggc tcgctcaatc ccgcgggcag
     1081 gtccaggccc ttcttcagga ggacggcggg gggcacgtcg gcgaagaggt cgtcgagctt
     1141 gggcacgccg acggcctgga gcatttcctt ctcgtcgtcc ggcgtgatgg ggatgtaggc
     1201 cactgaaact ccagttttgg gttcttagtt ttaagttcta aattaaagga cattttccca
     1261 aaggaaaatg gtcattaact caaaacttaa aactcaacac taatagctcc gcttttgagt
     1321 tagtggagcg tcgcgaggta ttgttcttcc gtcaggtagg cctcgagggc cttggcgtcg
     1381 tccaactgca ccacggccag ccatccggcg ccttccgggt cctgggtcac caggccggcc
     1441 tggtccttga gcttgtcgtt cacttcc
//
LOCUS       DASTJJ010000095         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_624634, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000095 DASTJJ010000000
VERSION     DASTJJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624634"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFR02_01170"
     CDS             <1..621
                     /locus_tag="VFR02_01170"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HET9869093.1"
                     /translation="EWELFSAVKKALEEGKPAITVEATPEQEKKLKELSLLTRKPFLF
                     IGNVAEGDLADVSKNAHYQALADYAKKQGASCLPISAKIEAEVAELPEDEAVVFLADL
                     GIKERGLHAIIRKSYETLGLISFFTAGPMESKAWTITKGTLAPQAAGKIHSDIERGFI
                     KAEVYSYDDLIANGSTAKAREAGKLRTEGKEYVMRDGDVVHFRFNV"
     gene            793..1386
                     /locus_tag="VFR02_01175"
     CDS             793..1386
                     /locus_tag="VFR02_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9869094.1"
                     /translation="MESLASNPDFVEISHWPGVVLDLKYASTDNFTHTNLYGDVQVAY
                     LHRLAADKFKKALASLQASHPGYKIVLFDTLRPRSVQWKLWNVVKGTPQQKYVADPEK
                     GSMHNYGFAMDIGLMDPQGHLVDMGAPFDGFTPLSEPQLEEQLLKEGKLTQRQIDNRM
                     ILRNAMVGAGFRQLRHEWWHYDALTEEQVRGKFQIVE"
     gene            complement(1519..2148)
                     /gene="nth"
                     /locus_tag="VFR02_01180"
     CDS             complement(1519..2148)
                     /gene="nth"
                     /locus_tag="VFR02_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9869095.1"
                     /translation="MSNSNTPAILKELKRLYPDVRCFLHYKTPFQLLCAVILSAQCTD
                     ERVNLVVPGLFKVFPTPEKLAKARIQDIEKLIKSTGFYHNKAKNLLGAAQALLALYKG
                     KMPRTIEEMTAIPGVGRKTANVVLGEVYGISEGITVDTHVTRLSNRLGWTRHQDAVKI
                     ERDLMGIVPKEEWLHFSHRLIQHGRKVCDARKPLCGACTLAKWCPSAKI"
     gene            2390..3370
                     /locus_tag="VFR02_01185"
     CDS             2390..3370
                     /locus_tag="VFR02_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9869096.1"
                     /translation="MIDVQDLSKIYRTQKRLPGIGGAIQGLFNRQFTEVRAVDGVSFS
                     IEEGELVGFLGPNGAGKTTTLKMLAGILYPTAGTARVMGFTPWERKNEYRRQFALVMG
                     QKNQLWWDLPAQESLYLNKEIYGVPDAEYNEVMDEMTELLQVKKLLGIMVRELSLGER
                     MKFELIASLLHRPRVLFLDEPTIGLDVVAQKNIREFIKQYNRRTKTTILLTSHYMNDI
                     QALCDRVVVINHGHKVFDGRLDDVVEKFSQSKLVTVSFKDGGISREKLAPLGDLVEHT
                     GVKAVLRVPRDRVKAASNDLLTHFDVDDINIEEVPIEEVIEKVFAGSQVA"
     gene            3363..>3578
                     /locus_tag="VFR02_01190"
     CDS             3363..>3578
                     /locus_tag="VFR02_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869097.1"
                     /translation="MPENAPFPLVKRPFFHKYWVILATRTQEDMAYRANYVFGAVFRF
                     LPLVTTIFLWWAVYQSRKQGGGPMSISG"
BASE COUNT          749 a         1064 c         1069 g          696 t
ORIGIN      
        1 gagtgggaac tcttctcggc ggtcaagaag gcgctggagg agggcaagcc cgccatcacg
       61 gtggaagcga cccccgagca ggagaagaag ctcaaggaac tgagcctgct gacccgcaaa
      121 cccttcctct tcatcggcaa cgtggccgag ggggacctgg cggacgtctc caagaacgcg
      181 cattaccagg ccctggcgga ctacgccaag aaacaaggcg ccagctgcct gcccatctcg
      241 gccaagatcg aggccgaggt ggccgaactg cccgaggacg aggcggtggt cttcctggcc
      301 gacctgggga tcaaagaaag gggcctgcac gccatcatcc gcaagtccta cgagaccctg
      361 gggctcattt ccttcttcac ggccgggccc atggaatcca aggcctggac catcaccaag
      421 gggaccctgg cgccccaggc ggcgggcaag atccattccg acatcgagcg gggcttcatc
      481 aaggccgagg tctattccta cgacgatctc atcgccaatg gctccaccgc caaggcgagg
      541 gaagcgggca aactgcgcac cgaaggcaag gaatacgtca tgcgcgacgg cgacgtggtg
      601 cattttaggt tcaacgttta aaaaacaaag acccaaccat gactttcacc accaaggcac
      661 caagaaagcc attttgggca agattcgaat tctaagatat tatgccgtcc ttggtgtctt
      721 ggtgccttgg tggtgatgat cggatttttt ctttgctgtt ttgctgccca agcccgctcg
      781 gtcgaggaat ccatggaatc actcgcgtcc aaccccgatt ttgttgagat ctcccactgg
      841 cccggggtag tgctggacct caagtacgcc tccacggata acttcaccca caccaacctc
      901 tacggcgacg tgcaggtggc ctatctgcat cggctggccg cggataagtt caagaaggcc
      961 ctggcttcgc tccaagcctc ccacccggga tacaagatcg tcctttttga cacgttgcgg
     1021 ccccggtcgg tccaatggaa gctttggaac gtggtcaagg gcacgcccca gcagaaatac
     1081 gtggccgacc ccgagaaggg ctccatgcac aactacggct tcgccatgga catcggcttg
     1141 atggaccccc aggggcatct ggtggacatg ggcgccccct tcgacggttt tacgccgctc
     1201 tccgagcccc agttggagga gcagttgttg aaggaaggga agctcaccca aaggcagatc
     1261 gacaaccgca tgatccttcg caacgccatg gtcggggcgg gtttccggca actcaggcat
     1321 gagtggtggc actatgacgc cttgaccgag gagcaggtca ggggcaagtt ccagatcgtc
     1381 gaataaaaga tcaaccaaag aaaaaagcca acgcaaagac gccaagacgc aaagttttag
     1441 aatgtttttt taaactagag tgaaaaggtt gagctttctt ggcgtcttag cgtcttcgcg
     1501 ttggcagttt ttgttggttt aaatcttggc tgaggggcac cacttggcca gggtgcaggc
     1561 gccgcagagg ggcttcctcg cgtcgcagac cttgcggccg tgttggatga ggcggtggga
     1621 gaagtggagc cactcctctt tcggcacgat gcccatcagg tcccgctcga tcttgaccgc
     1681 gtcctggtgc cgggtccaac ccaggcggtt ggaaagtctc gtcacgtggg tgtccaccgt
     1741 gatgccctcc gagatcccgt aaacctcccc cagcaccacg ttggcggtct tgcgccccac
     1801 cccggggatg gccgtcattt cctcgatggt cctgggcatc ttccccttat ataaagccag
     1861 cagggcctgg gcggcgccca gcaggttctt ggccttgttg tggtagaagc ccgtggactt
     1921 gatgagcttc tcgatgtcct ggatcctcgc cttggccagt ttctcggggg tggggaaaac
     1981 cttgaaaagc ccggggacga ccagattgac ccgctcgtcc gtgcattggg ccgacaggat
     2041 gacggcgcaa agcagttgga agggcgtttt gtaatgaagg aaacagcgca cgtccgggta
     2101 gaggcgcttg agttccttga ggatcgcggg ggtattggag ttcgacatat cgactaagct
     2161 tttaccacag agttcacaga gttacacgga gtaaggatcg aaagatcatt tattgggggc
     2221 agggtgaaaa tcaacggtgt ttttggcctc tggctttttc cgtactctgc gtaactccgt
     2281 gtgcatctat ggttccaggg gttcaatttc ttccgtcggg ccttggaaat cccgccgacg
     2341 gattcctata atacggccca ctacctccaa aggacgcccc ttgacgccca tcatcgacgt
     2401 gcaggacctc tccaagatct accgcaccca aaaacgcctg cccggcatcg gcggcgccat
     2461 ccagggcctc ttcaaccggc aattcaccga ggtgcgcgcg gtggacgggg tctccttctc
     2521 catcgaggaa ggggaactgg tcggtttcct gggccccaac ggggcgggga agaccaccac
     2581 gctcaagatg ttggccggca tcctctatcc caccgcgggc acggcccggg tcatgggctt
     2641 taccccctgg gaacgcaaga acgagtaccg ccgccagttc gcgctggtca tgggtcagaa
     2701 gaaccagctc tggtgggacc tgccggccca ggaatcgctc tacctgaaca aggagatcta
     2761 cggggtcccg gacgcggaat ataatgaagt gatggatgag atgaccgagc tgctgcaggt
     2821 gaagaagctc ctgggcatca tggtgcgcga gctgtcgctg ggggagcgca tgaagttcga
     2881 gctcatcgcc tcgctgctgc accggccccg ggtgctgttc ctggacgagc ccaccatcgg
     2941 gctggacgtg gtggcccaga agaacatccg ggaattcatc aagcaataca accgaaggac
     3001 caagaccacc atcctcctga ccagccatta catgaacgac atccaggcgc tctgcgaccg
     3061 ggtggtggtc atcaaccacg ggcacaaggt cttcgacggg cggctggacg acgtggtgga
     3121 gaaattctcc cagtccaagc tggtcacggt ctccttcaag gacgggggca tcagccggga
     3181 aaagctcgcc cccctgggcg acctggtgga gcacacgggc gtcaaggccg tgctgcgggt
     3241 gccccgggac cgggtgaagg cggcctccaa cgacctcttg acccatttcg acgtggacga
     3301 catcaacatc gaggaagtgc ccatcgagga agtcatcgag aaggtcttcg cggggtccca
     3361 agttgcctga gaacgcccct ttccccctgg tgaaaaggcc cttcttccat aagtattggg
     3421 tcatcctcgc cacccgcacc caggaagaca tggcctaccg ggccaactac gtcttcgggg
     3481 cggtcttccg tttcctgccc ctggtcacca ccatcttcct ctggtgggcg gtctaccagt
     3541 cgcgcaaaca gggcggcggg cccatgtcca tctcgggc
//
LOCUS       DASTJJ010000096         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1114779, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000096 DASTJJ010000000
VERSION     DASTJJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114779"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFR02_01195"
     CDS             <1..645
                     /locus_tag="VFR02_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9869098.1"
                     /translation="VFWVFPVVEESKTFLKSAVQMHQYFKNDVFHGFRVGLIHGKMKK
                     EEKEEAMRAFAAKETQILVATTVVEVGVDVANATVMVVEHAERFGLAQLHQLRGRVGR
                     GAAQAHCFLVTSPMMSAEAVKRVKIMAQTNDGFKLSEFDLQMRGPGEILGLAQSGRRE
                     GGLIDLRRDGALVERAREEAERRLAADPELASPENAALRQALKSRYDFQLASIS"
     gene            845..1435
                     /gene="rsmD"
                     /locus_tag="VFR02_01200"
     CDS             845..1435
                     /gene="rsmD"
                     /locus_tag="VFR02_01200"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET9869099.1"
                     /translation="MRVIAGKYRSREVKAPKGQAIRPTSDKVKGALFNMLEPLRDRRV
                     ADFYAGTGSLGIEALSRGARSVVFVDKGAEGLALIRENLGKLKIPQGPGGPAQVLALE
                     VGRAFAALSGEKKLFDVILADPPYEPGAARRVQGFLAQYPILAPGGVLAVEHGSVDLE
                     LDPDFPLPLVKQKKYGDTYLSLFRNDTEALHGDGGV"
     gene            1599..>1954
                     /gene="coaD"
                     /locus_tag="VFR02_01205"
     CDS             1599..>1954
                     /gene="coaD"
                     /locus_tag="VFR02_01205"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9869100.1"
                     /translation="MSKKIVGLYPGSFDPITNGHLDIARRALKLCDELHVSVVGNPSK
                     KALFPVEERVALLKKTLGRLPGVKIDSFNGLLVDYAREIGAGIIFRGLRAVSDFEYEF
                     QMALINRTLHPGVEVM"
BASE COUNT          363 a          558 c          655 g          378 t
ORIGIN      
        1 gtcttctggg tcttccccgt ggtggaggaa tccaagacct tcctcaagtc cgccgtgcag
       61 atgcaccaat acttcaagaa cgacgtcttc cacggcttcc gggtgggcct gatccacggc
      121 aagatgaaga aggaggagaa ggaagaagcc atgcgggcct tcgcggccaa ggagacccag
      181 atcctggtgg ccaccacggt ggtggaggtg ggcgtggacg tggccaacgc caccgtcatg
      241 gtggtggagc acgccgagcg cttcggcctg gcccaactgc accaactgcg gggccgggtg
      301 gggcgcggcg cggcgcaggc ccattgtttc ctggtgacct cgcccatgat gtcggcggag
      361 gcggtgaagc gggtcaagat catggcccag accaatgacg gcttcaaact ctccgagttc
      421 gacctgcaga tgcgggggcc cggggagatc ctggggttgg cccagtcggg ccgccgggaa
      481 ggcgggctga tcgacctgcg gcgcgacggg gcgctggtgg aacgggcgcg ggaggaggcc
      541 gaacggcgcc tggcggcgga ccccgaactg gcctcacccg agaacgcggc ccttcgccaa
      601 gcgctcaaaa gccgctatga tttccaactg gcctccattt cttgagcgcc aaaaccattt
      661 caccgcaaag gcgcaaagcc gccaaggaag tcgaaaagga ctggaagtca tttcaaagcc
      721 tcttttaccg tggaagcacg gagacgcaaa gttgagatga aggttaaaat cgggaaatgg
      781 atctgttttc tccgcgtctt ggcgtctttg cggtgaacag gttttatttt tgaatggagc
      841 tttcatgcgc gtgatcgcgg ggaagtaccg gagccgggaa gtgaaggcgc ccaagggcca
      901 ggccatccgg ccgacctcgg acaaggtgaa gggcgcgctc ttcaacatgc tggagcccct
      961 gcgggaccgc cgggtggcgg atttttacgc gggcacgggc agcctgggca ttgaggcgct
     1021 cagccgcggc gcccggtcgg tggtcttcgt ggacaagggc gccgagggat tggccctcat
     1081 ccgggagaac ctggggaagt tgaagatccc ccagggcccg gggggccccg cccaggtgct
     1141 ggcgctggag gtgggccggg ctttcgcggc cttgagcggc gagaaaaagc ttttcgacgt
     1201 catcctggcc gatccgccct acgaaccggg cgcggcccgg cgggtgcagg gctttttggc
     1261 ccaatacccg atcctggccc ccggcggcgt tctggcggtc gagcatgggt cggtggacct
     1321 ggaattggac ccggatttcc ccctgcccct ggtcaaacag aagaaatacg gggataccta
     1381 tttatccttg ttccggaacg acacagaggc gttgcacgga gacggcggag tttaaaaccg
     1441 agttttaaat ggggtaaaac tccgctttta actcggcgtc tccgtggaca gtttttggcc
     1501 gtaaaactcc gttttccccg tggaaagctt ttttgtgtag agctttgcct tgtcttttgc
     1561 atttgctaga ctggcccatc ccttgaacga ggtggctttt gtccaagaaa atcgtcggcc
     1621 tctatcccgg ctctttcgac cccatcacca acggccacct ggatatcgcc cggcgggcgt
     1681 tgaaactttg cgacgagctg cacgtgtcgg tggtggggaa cccctccaag aaggcgctct
     1741 tcccggtgga ggagcgggtg gcgttgttga agaagaccct gggaaggctt ccgggggtga
     1801 agatcgattc cttcaacggc ctgctggtgg attacgcccg tgagatcggc gccggcatca
     1861 tcttccgggg cctccgggcc gtctcggact tcgagtacga gttccagatg gcgctcatca
     1921 accgcacgct ccatcccggg gtggaggtca tgtt
//
LOCUS       DASTJJ010000097         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_503104, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000097 DASTJJ010000000
VERSION     DASTJJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503104"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1011
                     /locus_tag="VFR02_01210"
     CDS             178..1011
                     /locus_tag="VFR02_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET9869101.1"
                     /translation="MAPEERHDAVWKRFLKDKNAADRDFLARSYFFLVEAENRRLLAR
                     LPVKAYWEKKDDLASAGSIGLLQALDHFAPPKGKAGSPGRAFEAYARYRIRGQMVDEL
                     RHMDFARRNLRKQEKSIQEARYRLQQKNGREPTDEEAAKSLGMPLDEFYGVVAEINML
                     NLLSLDAELAGSRGEKGVWSDILPDPRAENALDKVEKQEKIEIVSAALKKLGDSEQKV
                     LQFYYVENLTFKEVGRVLGVSESRICQIHHMAVFKLQKMVDERREHDGAQHGRLYHHH
                     P"
     gene            968..2647
                     /locus_tag="VFR02_01215"
     CDS             968..2647
                     /locus_tag="VFR02_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HET9869102.1"
                     /translation="MTEPNMDGYITITHESDGVYLTVFPPQGQGVKPTLEDVKRELAK
                     YKVVPADEAVLASMVDKPTGTARKISSAASAAGQDQIFVEISDDEMAAKVTVVPPQSE
                     KEHFVVIDDVREALRRKSVVFGIDENKMTELSAKLARMAADKNLSEPVEAEVAFGTHV
                     KDGEDGRIDYLYKEKAQTLEAAQPVVEDEEGRVDYRAAHHIENIAKGTVLARKIPATK
                     GTPGMTVTGKATEAKDGKEIEVVAGKGVVASPDAKDEWIADTDGQVIIKDNKISVLAL
                     YEVAGDVNFSTGNIDFVGTVIVRGDVKDGFKVFAGEDLVINGVVEAAELKCNGKLTIG
                     GGVSGNDRAKIVCGGDANIKYVRNAQVEVAGDLTSAQAIMHCKVTVGRKVTVAGKKGV
                     IVGGQVIAGQEVMAASIGSNFATPTEIIVGEALGLRAEIQKIENDLKAAQENLDKTKK
                     GLVFLKDQQTKLGGNLPADKKELLTKLTRAQFKLMADVKNFAEKRQELEKKEQEGADE
                     RRRHAKVMCLGVIHNGVKITINKASRQIAEELKYCTLTESDGEIRVGPFKG"
     gene            2659..3219
                     /locus_tag="VFR02_01220"
     CDS             2659..3219
                     /locus_tag="VFR02_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869103.1"
                     /translation="MFFLILQTLFDAVMLAALVFIFHFTNHQMQKKKEEADLLKSLQV
                     QEIRDQLQELLMTLKQLGKEVSDDIQAQVLEAETKTELIKKITVKIQRDLKRTLVLAE
                     EVNAEKKRLEEKAQVLRVQPKKPAARAEEGLERMPVQDADMVRLVYRLADTQMDLDGI
                     AKQTKLTQAEIQLILNLRGNRFSTPN"
BASE COUNT          742 a          903 c         1067 g          508 t
ORIGIN      
        1 gtccatttcg cggggttggc ggggccggtg gcggccccgg cggcggcctc ggaaacgtcc
       61 gaacccccgg tggaggccga ggcgcctccc acgatcctgg agggggagga gaataagggg
      121 aaatcggtcg attacgtgtt ccccgaattt tcggccgaga accagccgta accggacatg
      181 gctccggagg aacgacacga cgccgtttgg aagcgctttc ttaaggataa gaacgccgcg
      241 gaccgggatt tcttggcgag gtcctacttc tttttggtgg aggccgagaa tcggaggctg
      301 ttggcccggt tgcccgtaaa ggcctattgg gaaaaaaaag acgacctggc ttcggcgggg
      361 tccatcgggt tgctccaagc gctggatcat tttgccccgc ccaagggcaa ggcgggttcc
      421 ccggggcgcg cttttgaggc ttacgcccgc taccgcatcc gggggcagat ggtcgatgag
      481 ctgcggcata tggacttcgc ccgcaggaac ctgcgcaagc aggaaaaatc gattcaagag
      541 gcccggtacc ggctgcagca aaagaacggg agggaaccca ccgacgagga ggccgccaag
      601 agcctgggca tgcctttgga cgagttttac ggtgtggtgg cggagatcaa catgctgaac
      661 ctgctttccc tggacgccga attggccggt tcgcgcgggg aaaagggggt ttggagcgac
      721 atcctgcccg atccgcgggc ggagaatgcg cttgataaag tggagaaaca ggagaaaatt
      781 gaaatcgttt cagcggccct gaaaaagctg ggcgattcgg aacaaaaggt cctgcagttc
      841 tactatgtgg aaaacctgac tttcaaggaa gtgggccggg tcctcggggt gagcgaatca
      901 cggatttgcc agattcatca tatggcggtt tttaaacttc agaagatggt ggatgaaagg
      961 cgggagcatg acggagccca acatggacgg ttatatcacc atcacccatg aaagtgatgg
     1021 agtgtatttg acggtcttcc cgccccaggg ccagggggtc aagcccaccc tggaggacgt
     1081 caaaagggaa ctggccaaat acaaggtcgt ccccgccgat gaagcggtgt tggcctccat
     1141 ggtcgacaaa cccacgggca cggcccgaaa gatatcctcc gcggcctccg ccgccggcca
     1201 ggaccagatc ttcgtggaga tctccgacga tgaaatggcc gccaaggtga ccgtggtgcc
     1261 cccccaaagc gaaaaggagc attttgtcgt catcgacgac gtccgggagg cgctgcggcg
     1321 caagagcgtg gtcttcggga tcgacgagaa caagatgacc gagctgtccg ccaagttggc
     1381 ccgcatggcc gcggacaaga acctgagcga accggtggag gccgaggtgg ccttcggcac
     1441 ccatgtgaag gatggggaag acggccggat cgattatctt tataaagaga aggcccagac
     1501 cctggaagcc gcccaacccg tcgtcgagga tgaggagggg cgcgtcgact atcgggcggc
     1561 ccatcacatc gagaacatcg ccaagggcac ggtgctggcc cgcaagatcc cggccaccaa
     1621 gggcaccccc ggcatgacgg tcaccggcaa ggccacggag gccaaggacg gcaaggagat
     1681 cgaggtggtg gcgggcaagg gggtggtggc ttcccccgac gccaaggacg agtggatcgc
     1741 cgatacggac ggccaggtca tcatcaagga caacaagatc tcggtcctgg ccctctatga
     1801 ggtggccggg gacgtgaatt tctccacggg caacatcgat ttcgtgggca cggtcatcgt
     1861 gcggggcgac gtgaaggacg gtttcaaggt cttcgccggg gaggacctgg tgatcaacgg
     1921 cgtggtggag gcggccgagc tcaagtgcaa cggcaaactg accatcggcg gcggcgtgag
     1981 cggcaatgac cgcgccaaga tcgtgtgcgg cggggacgcc aacatcaagt acgtgcgcaa
     2041 cgcccaggtg gaagtggccg gggacctgac ctccgcccag gccatcatgc attgcaaggt
     2101 cacggtcggg cgcaaggtca ccgtggcggg caagaagggc gtgatcgtgg gcggccaagt
     2161 gatcgcgggg caggaggtca tggcggcttc catcggctcc aatttcgcca cccccaccga
     2221 gatcatcgtg ggcgaggcct tggggcttcg ggccgagatc cagaagatcg agaacgacct
     2281 gaaggcggcc caggagaacc tggacaagac caagaaaggc ctggtcttcc tgaaggacca
     2341 gcagaccaag ctgggcggca acctgcccgc ggacaagaag gaactcttga ccaaactgac
     2401 ccgggcccag ttcaagttga tggcggacgt caagaacttc gccgaaaagc ggcaggaact
     2461 ggagaagaag gagcaggagg gcgccgacga gcgccgccgc cacgccaagg tcatgtgcct
     2521 gggcgtcatc cacaacgggg tgaagatcac catcaacaag gcttcccggc agatcgccga
     2581 agagctgaaa tactgcacct tgaccgagtc cgacggcgaa atccgggtcg gacccttcaa
     2641 gggctgaccg ggacccccat gttcttcctc atcctccaga ccctcttcga cgcggtgatg
     2701 ttggccgcgt tggtcttcat cttccatttc accaaccacc agatgcagaa gaaaaaggaa
     2761 gaggccgacc tcttgaagag cctgcaggtc caggagatcc gggaccagct ccaggaactg
     2821 ctcatgacct tgaagcagtt gggcaaggag gtctcggacg acatccaggc ccaagtgttg
     2881 gaggcggaga ccaagaccga attgatcaag aagatcaccg tgaagatcca acgggacctg
     2941 aagcggaccc tggtcctggc cgaggaggtc aacgccgaga aaaaacgcct ggaagaaaag
     3001 gcccaggtgc tgcgcgtgca gcccaaaaaa ccggcggccc gggcggaaga gggcctggag
     3061 cgcatgcccg tgcaggacgc cgacatggtg cgcctggtct atcgattggc cgacacccaa
     3121 atggacctgg acgggatcgc caagcagacc aaattgaccc aggccgagat ccaactgatc
     3181 ctgaacttac gcggcaaccg tttttccacc ccgaattgat
//
LOCUS       DASTJJ010000098         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_624823, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000098 DASTJJ010000000
VERSION     DASTJJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624823"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFR02_01225"
     CDS             complement(<1..891)
                     /locus_tag="VFR02_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET9869104.1"
                     /translation="MKPSFALLAGLLLILSGPGRLWAWGDYGHQVIAQIALDNLSAPV
                     SAKISEMINDAGYTSPDQLVLDGLGEHDSQEGFPSHATMLEISVWPDYLLTRSKEFNT
                     MTAPWHYISIDRNVGETEEKEAAFCRNNDCLPAQVNQALSNLTDDSISVKKKFVSLAM
                     LINLMGDIHEPLNCSDDGDHNGTEKYFTWNGASPLSLHSLWNNLLRAAPPTGDPKAYA
                     RTLEKDITDDDRQAWQSGVASDWAWESYQVADEKIYALPQFHYGPQNVKVDSLGELVY
                     RGGKPAVTRSVPFPEEFHRDE"
     gene            903..1085
                     /locus_tag="VFR02_01230"
     CDS             903..1085
                     /locus_tag="VFR02_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869105.1"
                     /translation="MEWSNPLILSARGRPHKEQIARKAQGFHRQDAKDAKKGKVKTHF
                     FNGGKLSGAVTIGWLS"
     gene            complement(1142..>1987)
                     /gene="guaA"
                     /locus_tag="VFR02_01235"
     CDS             complement(1142..>1987)
                     /gene="guaA"
                     /locus_tag="VFR02_01235"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9869106.1"
                     /translation="IHRAVGTQLDCVFVNNGVLRKDEARQVVAMFRSLGIPLHYINAE
                     DRFLKLLKGVAEPERKRKIIGNTFIRVFEQKARELQKKKGPFQFRAQGTLYPDVIESV
                     SVKGPSAVIKSHHNVGGLPKDMRFKLVEPLRELFKDEVRKLGKKLGIPHGMLWRQPFP
                     GPGLAVRHIGALTKERLDVLREADAIVQEEILKAGLTHAIWQSFAVLTPVKSVGVMGD
                     ERTYESACVIRAVHSTDGMTADWVKLPYELLGRMSNRIINEVRGINRVVYDITSKPPG
                     TIEWE"
BASE COUNT          340 a          588 c          654 g          405 t
ORIGIN      
        1 ctcgtcccgg tggaactctt ccgggaaagg cacggagcgc gtgacggcgg gcttgccccc
       61 ccggtagacc aattccccca agctgtccac cttgacgttc tgggggccgt agtggaactg
      121 aggcagcgcg tagatcttct catcggccac ttggtagctt tcccaggccc aatcggacgc
      181 caccccggac tgccaagcct ggcggtcgtc gtccgtgatg tccttttcca gcgtgcgggc
      241 gtaggccttg gggtcgcccg tggggggcgc ggcgcgcagc aggttgttcc agagcgaatg
      301 caggctcaag ggcgacgcgc cgttccaggt gaagtacttt tcggtgccgt tgtggtcgcc
      361 gtcgtccgag caattgagcg gctcgtggat gtcgcccatc aggttgatga gcatggccag
      421 ggagacgaac ttcttcttca cggagatcga gtcatccgtc aggttggaca gggcctggtt
      481 cacttgggcg ggcaggcagt cgttgttgcg gcagaaggcg gcctcttttt cctcggtctc
      541 ccccacgttg cggtcgatgg agatgtagtg ccagggcgcc gtcatggtgt tgaattcctt
      601 cgaacgggtg agcaggtagt cgggccagac cgagatctcc agcatggtgg cgtgggaggg
      661 gaagccttcc tggctgtcgt gctcacccag gccgtccagg accagttggt cgggggaagt
      721 gtagcccgcg tcgttgatca tctcggagat cttcgccgag accggggcgc tgaggttgtc
      781 cagggcgatc tgggcgatga cctggtggcc gtagtccccc caggcccata ggcgtcccgg
      841 acccgaaagg atgaggagca gaccggccag caaggcgaag gatggtttca tggagacccc
      901 cggtggaatg gtcgaaccct ttgattttat cggcccgggg gcggccccac aaggaacaaa
      961 tcgcacgaaa agcccaaggc tttcaccgcc aagacgccaa ggacgccaag aaaggcaaag
     1021 tcaaaacaca tttttttaat ggcggaaaac tttcaggtgc cgtcaccatt ggttggttgt
     1081 cttgaagttc ttggcgactt ggcgtcttgg cggtgaaaag ggttctaatg gatagattgc
     1141 cctattccca ctcgatggtg cccggcggct tgctggtgat gtcatagacc acccggttga
     1201 tgccccgcac ctcgttgatg atgcggttgg acatgcgccc cagcagctcg tagggcagct
     1261 tcacccagtc ggcggtcatg ccgtcggtgg aatgcacggc ccggatcacg caggcgctct
     1321 cataggtgcg ctcgtcgccc atgaccccca cggacttgac gggggtcagc accgcgaagg
     1381 actgccagat ggcgtgggtg aggcccgcct tgagtatctc ctcctgcacg atggcgtcgg
     1441 cctcgcgcaa cacgtccagc cgctccttgg tcaaggcgcc gatatggcgc acggccaacc
     1501 ccgggcccgg gaagggctgg cgccagagca tgccgtgggg gatgccgagc ttcttgccca
     1561 gtttccgcac ctcatccttg aacagttccc gcaggggttc cacgagcttg aagcgcatgt
     1621 ccttgggcag cccgcccacg ttgtggtggc tcttgatgac ggccgagggg cccttcacgg
     1681 acacgctttc gatgacgtcc ggatagaggg tgccttgggc ccggaattgg aaagggccct
     1741 tctttttttg gagctcccgg gccttttgct cgaagacccg gatgaaggtg ttgccgatga
     1801 tcttgcgctt gcgctcgggt tcggccacgc ccttcaagag cttgaggaac cggtcctcgg
     1861 cgttgatgta atgcagcggg atgcccaggc tgcggaacat ggccaccacc tgccgggcct
     1921 cgtccttgcg caacacgccg ttgttgacga acacgcagtc caattgcgtc ccgaccgccc
     1981 ggtggat
//
LOCUS       DASTJJ010000099         1400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_381144, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000099 DASTJJ010000000
VERSION     DASTJJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381144"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFR02_01240"
     CDS             complement(<1..570)
                     /locus_tag="VFR02_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869107.1"
                     /translation="MNAVCAWWKRLAAEEAEEILLFQAERARILRALLLLGWLMTTFF
                     MALTLVKGRHFPWLPLVTDSDLLALAFWVERRPQDHRWISRLLLFPAVAFILVDAAGW
                     PPDLLAPALVFLPTLAYFAVLLDGPRAGLVMAVLEAAGLLFYDLRNPLPGREALIART
                     NLLLVVPGYLVLGWVAWGRFRDLGRAVTAR"
     gene            772..1362
                     /locus_tag="VFR02_01245"
     CDS             772..1362
                     /locus_tag="VFR02_01245"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET9869108.1"
                     /translation="MPYTLPQLPYAKDALAPHLSAETFDYHYGKHHQAYVDNLNKLVS
                     GTEFEGKTVEEIVKKAVGGLFNNAAQHWNHSFYWNSLSAEKNQSPKGALAEAITKSFG
                     SQAEFEKQFSAAAMGQFGSGWAWLVAGPGKTLKIETTGNAETPAHTGSGTPLFTCDVW
                     EHAYYIDYRNARAKYVEAFWKVLNWDFAGKNFDAAK"
BASE COUNT          286 a          460 c          477 g          177 t
ORIGIN      
        1 ccgggccgtg accgcccgtc ccaggtcgcg gaaacggccc caggccaccc agcccagcac
       61 caggtagccc ggcaccacca gcagcaggtt ggtgcgcgcg atgagggcct cccggccggg
      121 caggggattg cgcaggtcat agaagagcaa gccggcggcc tccaagaccg ccatcaccag
      181 gcccgcccgg ggcccgtcca gcagcacggc gaaataggcc agggtgggca ggaagaccag
      241 ggcgggggcc agcaggtccg gcggccagcc ggcggcgtcc accaggatga aggccaccgc
      301 ggggaagagc agcaggcggg agatccagcg gtggtcctgg gggcggcgct cgacccaaaa
      361 ggccaaggcc aggaggtcgg agtcggtcac cagcggcagc cagggaaaat gacggccctt
      421 caccagggtg agggccatga agaaggtggt catgagccag cccaacaaga gcagggcccg
      481 gaggatgcgg gcgcgctcgg cctggaagag gaggatctcc tcggcttcct cggcggccag
      541 ccttttccac caggcgcaga cggcgttcat gttcgtcctt tggggtgagg aagatgggcg
      601 gtgggattct aggacaagga ccaaatttgc gtcgatactt atattttaag cccgtcccct
      661 cccgcgggaa aagggaaggg gcgcgaaaac ttcccgcgcg cccttgactt cgcccggagg
      721 gggcgcttca atcgggctga ggacgaattc catcccaaac gaggagagcc catgccttac
      781 accctgcccc aactgcccta cgcgaaggac gccctggcgc cccacctgtc ggcggagacc
      841 ttcgactacc actacggcaa gcaccaccag gcctacgtgg acaacctgaa caagctggtg
      901 tccggcaccg aattcgaggg gaagacggtg gaggagatcg tgaagaaggc cgtcgggggg
      961 ctgttcaaca acgccgccca gcattggaac cattccttct attggaacag cctgagcgcc
     1021 gagaagaacc agtcgcccaa gggcgcgctg gccgaggcca tcaccaagtc cttcgggtcc
     1081 caggccgagt tcgagaagca gttcagcgcg gccgccatgg gccagttcgg ctccggctgg
     1141 gcctggctgg tggcgggccc cggcaagacg ctcaagatcg agaccacagg caacgccgag
     1201 accccggccc acaccggatc gggcacgccg ctcttcacct gcgacgtgtg ggaacacgcc
     1261 tattacatcg actaccgcaa cgcgcgggcc aagtacgtgg aggccttctg gaaggtcctc
     1321 aactgggact tcgcgggcaa gaacttcgac gccgccaagt agggcccccg ccgcgaccgg
     1381 ccgtcccggg acccccgggg
//
LOCUS       DASTJJ010000100         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1726943, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000100 DASTJJ010000000
VERSION     DASTJJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726943"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..577
                     /gene="aroQ"
                     /locus_tag="VFR02_01250"
     CDS             131..577
                     /gene="aroQ"
                     /locus_tag="VFR02_01250"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9869109.1"
                     /translation="MRKVLVIHGPNLNLLGEREPQVYGSVTLDEINREMGEFAAKNGI
                     ELKVVQSNHEGEIVEAIQNSRNWADVLIINPAAYTHTSVAIRDALAAVKIPAIEVHLS
                     NIHAREGFRQMSHVSPVALGVVSGFGKNSYLLALQAAVLVPLPEKK"
     gene            574..>1174
                     /locus_tag="VFR02_01255"
     CDS             574..>1174
                     /locus_tag="VFR02_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869110.1"
                     /translation="MSLAASFLNRPLSRDLLWEAGALGAGLFLLARPPLAHPQSFAFA
                     LGAGLFLLLWRTPGPGALGTLGPAGCCSLLYTLLASAWSLSPGLSWQSASFLFLGAAL
                     ALMASSSSPEGKSRLELFGFLAALAAALWGLEQVLWGYDRWAAQAPRLSGLDLTALHD
                     AERGRRAFGPLVTPGALAGLLILFIPWGFLQAWTRRGLSR"
BASE COUNT          154 a          403 c          377 g          240 t
ORIGIN      
        1 gggggaaggt tcgggtggcc atggggcggg attatagcct cccggccggc ccggtggggc
       61 gcctttttgc ttgtgcgggc tccctctttc tgattagcat gattgcacct cggcttccaa
      121 ggaaacccac atgcggaaag tattggtcat ccacggcccc aacctgaacc tgctggggga
      181 gcgtgagccc caggtctacg ggtccgtcac cttggacgag atcaaccggg agatgggcga
      241 gttcgccgcc aagaacggga tcgagctcaa ggtcgtccag tccaaccacg aaggcgagat
      301 cgtcgaggcc atccaaaatt cccggaattg ggcggacgtc ctcatcatca atccggcggc
      361 ctacacccac acctccgtgg cgatccggga cgccttggcc gcggtgaaga tccccgccat
      421 cgaggtgcac ctgtccaaca tccacgcgcg ggaaggcttc cggcagatgt cccacgtgtc
      481 gccggtggcc ttgggggtgg tgtccggttt cgggaagaac agctacctcc tggccctgca
      541 ggccgccgtc ctggtccccc tgcccgagaa aaagtgagcc ttgcggcctc tttcctgaac
      601 cggcccctgt ccagggatct tctctgggag gccggggccc tgggggcggg gcttttcctg
      661 ttggcgcgcc cccccctggc ccatccccaa tccttcgcct tcgctttggg cgccgggctt
      721 ttcctcctct tgtggcgcac cccgggcccg ggggccctgg ggaccctggg cccggccggc
      781 tgttgttccc tcttatatac actcttggct tcggcctggt ccttgagtcc gggcttgagc
      841 tggcaatcgg cttccttcct ctttctgggg gcggccctcg ccttgatggc gtccagctcc
      901 agtcccgaag gcaaatcccg gctggaactt ttcggtttcc tggccgccct ggccgcggcc
      961 ctctggggat tggagcaggt cctttggggt tacgaccgct gggccgccca agcgccccgc
     1021 ctttccggcc tggacctgac cgccctgcat gacgccgaaa ggggccgccg ggccttcggt
     1081 cccttggtca cgcccggggc cttggcggga ttgctgatcc tcttcatccc gtggggcttc
     1141 ctccaggcct ggacccggcg gggcctgtcg cgga
//
LOCUS       DASTJJ010000101         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_259353, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000101 DASTJJ010000000
VERSION     DASTJJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259353"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..249
                     /locus_tag="VFR02_01260"
     CDS             85..249
                     /locus_tag="VFR02_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET9869111.1"
                     /translation="MTEESDREVLEESYVTRERLELAAYYRWRGRGCPMDDPLTDWVG
                     AHKEMIQGAR"
     gene            180..620
                     /locus_tag="VFR02_01265"
     CDS             180..620
                     /locus_tag="VFR02_01265"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9869112.1"
                     /translation="MPDGRSLDGLGGGAQGNDPRRPLNPRRALKGSPAAQRNPGVGPR
                     RPAPKVGRPLSAAAVKGHLAQMPGWKLSGKSIVRTFAFGDFYRTMGFVNALAYVAHRG
                     NHHPDLEVGYGSCKVRYSSHELGGLSEKDFLCAAQVDGLLEGRP"
     gene            complement(631..801)
                     /locus_tag="VFR02_01270"
     CDS             complement(631..801)
                     /locus_tag="VFR02_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HET9869113.1"
                     /translation="MTAGILGLAIFISDIVAIAIVLQSRMGTGRKFSWCLLILMLPVA
                     GWLLWFFAAPRK"
     gene            complement(847..1008)
                     /locus_tag="VFR02_01275"
     CDS             complement(847..1008)
                     /locus_tag="VFR02_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869114.1"
                     /translation="MGSSRRSTGLEGGTNLVYIILGVALTVGALSLLGYIHHENRDIE
                     IHPPHVEVR"
BASE COUNT          261 a          367 c          430 g          241 t
ORIGIN      
        1 gaggttccta tctattccac ggcggctagc ctactctgga cgggcgcccg ttgcggaccg
       61 cgggccggga aacggatgga cgacatgacg gaagaatcgg accgggaagt tttggaagaa
      121 agctacgtga cgcgggaacg cctggaactg gcggcctatt atcggtggcg gggccggggg
      181 tgcccgatgg acgatccctt gacggactgg gtgggggcgc acaaggaaat gatccaaggc
      241 gcccgttgaa tccccgccga gccttgaagg gatcgccggc tgcgcagcgg aaccccgggg
      301 tcggaccccg tcgacccgcc cccaaggtcg gccggcccct gtccgccgcg gcggtcaagg
      361 gtcatttggc gcaaatgccc ggatggaaac tatccgggaa gtccattgtc cggaccttcg
      421 ccttcgggga tttttatcga acgatgggtt ttgtcaacgc cttggcctat gtcgcccatc
      481 ggggaaacca tcacccggac ctggaagtgg gctatggatc ctgcaaggtg cggtacagtt
      541 cccatgaact cggcggttta tcggaaaagg acttcctctg tgccgcccaa gtggatgggc
      601 tcttggaggg acggccctga aaagagccgt tcatttccgg ggggcggcga aaaaccaaag
      661 gagccatccg gccaccggga gcatgaggat caacaggcac cacgaaaatt tccggcctgt
      721 tcccatccgg ctttggagga cgatggcgat ggccacgata tcggagatga atatggccaa
      781 acccagtatg ccggcggtca tttttccctc ccggacggtt tcaaaggcgg ggcgaagttt
      841 tgcccgtcaa cgcacttcca catggggagg atggatctcg atgtcgcggt tctcgtggtg
      901 gatgtagccc agcagcgaaa gggcgccgac cgtcagcgcg acccccagga tgatatagac
      961 caggtttgtc ccgccttcga ggcctgttga ccggcgtgag gatcccatga ctttcctttt
     1021 gaaaatgaaa tcagaaccca agcttcaagg aacttgaagt gcaagtgccg tgcctggata
     1081 tatcctccgg cggccgggca aaagcccttt tccgggggac ggccgggcgg accctcaaaa
     1141 tgacgcgcgg tgccctatcg ttttgatcgc ggcgggatta tttccggttt ttgcgggacg
     1201 ggggggggga caaacgccgt ccgcgcgccc atgatgaggg gagccgcatt cattcccggg
     1261 agaggggccg accgtgacca agcacgacta tgagcgccg
//
LOCUS       DASTJJ010000102         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_382712, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000102 DASTJJ010000000
VERSION     DASTJJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382712"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(524..>1333)
                     /locus_tag="VFR02_01280"
     CDS             complement(524..>1333)
                     /locus_tag="VFR02_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9869115.1"
                     /translation="AKLFMRHDFRHIDPAKARVFLIEGGPRILAAYPEELSRKAVEQL
                     TSLGVEVYTGLQVTDVQPGYVAAGDRRLDSVVTLWAAGVQPSPLGKLLGVETNRRGQV
                     IVNQTLNPTGHPEIFVCGDLSHFEQDGHQLPGVAQPAMQMGDHVGKMIAEDLARQPRT
                     PFRYFDKGDVATIGRMAAVAHLKWPIKANLSGFPAWFTWLTIHLFFLIGFRNRLVVLI
                     DWVWNYFTFTRGARLIYGSENLPGWTRQQGVQPVAETAPLDETSPGTRGGA"
BASE COUNT          272 a          350 c          476 g          235 t
ORIGIN      
        1 atgcccatta tcgtgacgga cgtaatatcc ataagcgcgt ccgtggtccc agcggtggtc
       61 aggccgcggg tgcggacgac ggacccagta tccacggtag taccttcctc cgccggaacg
      121 tatgaagcga tgggcccccc agccgtgggc gtagccccaa ttggcccagg ggccgacgcc
      181 gaggaagatt ccgttataga agtagcccgg tccatagaag cccggagggg cgcaggagta
      241 aggagcaaac tcgtaatatc cgtaggggca gacgggctgg gtgccgatct gcacgctgag
      301 ggaaacctgg gcatcggcct tgggcgcgaa agcaaaaaac ggaagcgcca gggccgcgca
      361 gagaagcgcg agggttccgc gccggaggag gggttttcgt tcaggaatca tattcaaaag
      421 gacaccgttc gaccacaata gttgtggcca ataggtgata ttggcacgaa ccacggcgct
      481 aaggaaagac catttttcgg cccggaacct ctttggaact cgtttaggca ccgccccttg
      541 taccgggaga ggtttcgtcg aggggggcgg tttccgcaac aggctggacg ccttgctggc
      601 gggtccatcc ggggagattt tcgctgccgt agatgaggcg cgccccacgg gtaaaggtga
      661 agtagttcca gacccaatcg atcaggacca ccaatcggtt tcggaagccg atgaggaaga
      721 agaggtggat ggtgagccag gtgaaccagg cggggaatcc gcttaggttg gccttgatgg
      781 gccacttgag atgggcgacg gcggccatgc gaccgatggt ggccacgtct cccttatcga
      841 agtagcggaa gggtgtgcgg ggctggcgag cgaggtcttc ggcaatcatt ttgccgacgt
      901 ggtcgcccat ctgcatggcg ggctgggcga ctccagggag ttgatgcccg tcctgctcga
      961 agtgggagag gtcgccgcag acaaagattt ccgggtgccc cgtcgggttg agtgtctggt
     1021 ttacgatgac ctggccgcgg cggttggttt ccacgccgag cagtttgccg agtggggaag
     1081 gctgcacgcc ggcggcccaa agagtaacga cggagtcgag gcggcggtct ccggcggcga
     1141 cataaccggg ctggacatcg gtgacctgga gcccggtgta gacctcgacg ccgagcgagg
     1201 taagctgctc gacggccttg cgggaaagtt cttcgggata ggcggcgaga atgcgcgggc
     1261 cgccttcgat gagaaagacg cgggccttgg ccggatcgat gtggcggaaa tcatgccgca
     1321 taaagagctt ggc
//
LOCUS       DASTJJ010000103         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1238884, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000103 DASTJJ010000000
VERSION     DASTJJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238884"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFR02_01285"
     CDS             <1..204
                     /locus_tag="VFR02_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869116.1"
                     /translation="DIHLIREPHWHIGMIYFARVKGGTTTLNEGEHRDIRWLGESDLQ
                     DGAWGLSAPLKFYAREALRKVKT"
     gene            complement(335..766)
                     /locus_tag="VFR02_01290"
     CDS             complement(335..766)
                     /locus_tag="VFR02_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HET9869117.1"
                     /translation="MRVDWHAYFMNIAREVATRSTCDRKHVGAVIVRDKTILSTGYNG
                     SVRGMPHCDDAGHMMEDGHCVATIHAESNAVIQAARNGVRLEGSEIYVTASPCWNCFK
                     MLANAGIKTIYYGEFYRDDRIFDAAKRLGILTTLIPVKKPR"
     gene            complement(1021..1935)
                     /locus_tag="VFR02_01295"
     CDS             complement(1021..1935)
                     /locus_tag="VFR02_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869118.1"
                     /translation="MSLVALPHADFFDRVDPLDADLEAAEALYAGPFLTASLPAFLMW
                     QDEFLYADAGLAGARFLVAHYDKYVYLPTPPRPFTRENVEKAFAYMRAVNGPGPGISR
                     IEGLAESQKDQGRSWGFPTRPTLVEYVYERAKTAALAGDAYRSQRAEVNHLVKHHPVL
                     FRPYRRADFPACGDLFEAWKAQRLPALKGGMGESMLLASQRAHARALRQGEAWGLKAW
                     VVLLGGRLAAYTVGGALDADTFGVHLEVADLTVRGLSAYIFRNLCRQMEPYAFINTGD
                     AEGLPRLAESKDHWHPFRKPEIFALDPA"
     gene            complement(2196..>2583)
                     /locus_tag="VFR02_01300"
     CDS             complement(2196..>2583)
                     /locus_tag="VFR02_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET9869119.1"
                     /translation="KYLRGRIAGDVMDREWTLHKRARKTRSRYKRGFYNPLKQVATQI
                     YHLRSHLENNLGPRWAHECSQIWIQGVVVFAHPQGDASAVVNHAHGVVTLDELPKYLA
                     NYRPPSRAHRLSYGLRREITRVLTGQ"
BASE COUNT          452 a          774 c          865 g          492 t
ORIGIN      
        1 gacatccacc tcatccggga gccccattgg cacatcggca tgatctattt cgcccgggtg
       61 aaggggggca cgaccaccct gaacgagggg gagcaccggg acatccggtg gctgggggaa
      121 agcgaccttc aggatggggc ctggggcctg agcgcgccgc tgaaatttta cgcgagggaa
      181 gccctgcgaa aagtgaagac ctgaaccccg gccggctctt taccacgaag tccatcgagt
      241 caggggaaat ggtgtttcag gttttgaggg gatcggcctt acttcgtgcc cttcgtgaac
      301 ttcgtgaact tcgtggtaaa agcttcaggt ttttttacct gggttttttg acggggatga
      361 gcgtggtgag gatgcccagg cgcttggccg cgtcgaagat gcggtcgtcc cggtagaatt
      421 ccccgtaata gatggtcttg atgcccgcgt tggccagcat cttgaagcag ttccagcagg
      481 gcgaggccgt cacgtagatc tccgagccct ccagccgcac gccgttgcgg gccgcctgga
      541 tgaccgcgtt ggattcggcg tggatggtgg ccacgcaatg cccgtcctcc atcatgtgcc
      601 ccgcgtcgtc gcagtggggc atgccccgca cgctgccgtt gtagccggtg gacaggatgg
      661 tcttgtcgcg cacgatgacc gcgcccacgt gcttgcggtc gcaggtggac cgggtggcca
      721 cttcccgggc gatgttcatg aaataggcgt gccagtccac ccgcacccgg tgggtggggg
      781 cggcggcgtt gggtttcgcc gcgcggcggc tcaagcgggc cagggccttc ttcttcaatt
      841 tcaccttcac acgggcgctc atggtcttcc tttcagaact ggataagtcg aagatccgat
      901 cttcaccacc aaggagaaga tcttggcctt caagagggtc ggtctttctt ggcgaacttg
      961 ccggccgacc ccgctttggc ttaagccttc atttcttgaa cggcaacatt cctttaggga
     1021 tcaggcggga tcgagggcga aaatttccgg cttgcggaag ggatgccaat ggtccttcga
     1081 ttccgccagc cggggcaggc cctcggcgtc gccggtgttg atgaaggcgt agggttccat
     1141 ctgccggcaa agatttcgga atatataagc cgacaatccc ctcaccgtca agtccgccac
     1201 ctccaggtgg acgccgaagg tgtccgcgtc caacgccccg cccaccgtat aggccgccag
     1261 gcgcccgccc aacagcacca cccaagcctt caggccccag gcctcgccct ggcgcagggc
     1321 cctcgcgtgc gcgcgctggg aggcgagcag catggattcc cccatgccgc ccttcagggc
     1381 cgggagccgc tgggccttcc acgcctcgaa caggtccccg caagccggaa aatccgcccg
     1441 gcggtaggga cggaagagga ccggatggtg cttcaccagg tggttcacct ccgcgcgctg
     1501 ggaccggtag gcgtcgcccg ccagggcggc cgtcttggcg cgctcataga cgtattccac
     1561 cagggtggga cgggtgggga aaccccagga ccggccctgg tccttttggc tttcggccag
     1621 gccctcgatg cgggaaatcc ccgggcccgg cccgttgacc gccctcatat aagcgaaggc
     1681 cttttccacg ttctcccggg tgaagggccg gggcggcgtg ggcagataga cgtatttatc
     1741 gtaatgggcc accaggaaac gggcccccgc cagcccggcg tcggcataga ggaactcgtc
     1801 ctgccacatg aggaaggcgg gcagggaagc ggtgaggaag gggccggcat aaagggcttc
     1861 ggcggcctcc aggtcggcgt ccagggggtc gacccggtcg aaaaaatccg cgtgggggag
     1921 ggcaacgagg ctcatgggtc cattttcagg ctttgggctt taaaggccgt ttcagaacct
     1981 cttttaccgc aaaggcgcag agacgcaaag ggaaagccgg ggtcaaagac cagggttaat
     2041 aaatgcgttt ttccctgtgc ctttccggtg gaaaaaccag ctgtttgggg ttttgaaaca
     2101 ggtcttaaat tctgagtgac ggccgttgcc cgccggggat cttcccgtcg ggcaaggccg
     2161 ctgctcaaaa ccaagatctt cgaaatagga ccggcttatt ggccggtcaa gacccgggtg
     2221 atctcccggc gcagcccgta gctgaggcgg tgcgcccggg aaggcggccg gtaattggcc
     2281 aggtacttgg gcagctcgtc cagggtgacc acgccgtggg cgtggttcac caccgccgag
     2341 gcgtcgcctt ggggatgggc gaagaccacc acgccctgga tccagatctg ggagcattcg
     2401 tgcgcccagc gggggcccag gttgttctcc aggtggctgc gcaggtggta gatctgggtg
     2461 gcgacctgct tcagggggtt gtagaagccc cgcttgtagc gggaccgggt cttgcgggcc
     2521 cgcttgtgca gggtccattc gcggtccatc acgtcgcccg cgatgcggcc ccggaggtac
     2581 ttg
//
LOCUS       DASTJJ010000104         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_383676, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000104 DASTJJ010000000
VERSION     DASTJJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383676"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFR02_01305"
     CDS             <1..387
                     /locus_tag="VFR02_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869120.1"
                     /translation="GTLPDASDAMAAGDPGQMTYSYTVTMDYPAQAALDFYGQKMAAK
                     GWSPLNTNMFGPPDQWGSSPNVDPETRKPACEFKYVTVWASQDKGRVAVLALVYFDPS
                     ANGACAATPKVRRLRVTLEELPMPKG"
     gene            485..1015
                     /locus_tag="VFR02_01310"
     CDS             485..1015
                     /locus_tag="VFR02_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9869121.1"
                     /translation="MSKAFWAGVGTAVVAGLVVGYLSVILGLMPANADSKPGKLETWM
                     AHHSLRATLRREAPPGDNPLPADAADLKAGLRLYSLNCAVCHGASDARASHIAMGLYQ
                     HAPQLAQHGVEDDPDGVIYWKIAHGIRLTGMPSFDGALTEKQLWQLTLFLKHMDALPP
                     AVQKSWKALPSAAVKD"
     gene            1081..1371
                     /locus_tag="VFR02_01315"
     CDS             1081..1371
                     /locus_tag="VFR02_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9869122.1"
                     /translation="MKLPAPQIEKSAGCIKAMAHPLRLSILALLQEGEKNVQDLTQAL
                     GAGQSNVSQHLAQMRARGLVTARREGNMVYYAVGHPKIARLMALMREVFCGE"
     gene            1423..1578
                     /locus_tag="VFR02_01320"
     CDS             1423..1578
                     /locus_tag="VFR02_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HET9869123.1"
                     /translation="MRTLAAVALGALLGLAWWRFVGCSSGACPITSNPWASALYGAGL
                     GFLMSRP"
     gene            1661..1864
                     /locus_tag="VFR02_01325"
     CDS             1661..1864
                     /locus_tag="VFR02_01325"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9869124.1"
                     /translation="MKNVGRYDREVRFIAAALLAVLGYLESGTARLILWVLMLVPLFT
                     GLFRYCPLWTVFKIDTYREKRGR"
     gene            1861..>2100
                     /locus_tag="VFR02_01330"
     CDS             1861..>2100
                     /locus_tag="VFR02_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9869125.1"
                     /translation="MSPFSLMGLFAGRPDPASLDPQQVRERLAADPKSQLVDVRSHEE
                     RREGYLKGSKPIPLPELSGRLKELDPNRPVLLYCRS"
BASE COUNT          360 a          645 c          715 g          380 t
ORIGIN      
        1 gggacgctcc cggacgcctc ggacgccatg gcggccgggg accctggaca aatgacctat
       61 tcctatacgg tgacgatgga ttatccggcc caggcggcgc tcgactttta cgggcaaaag
      121 atggccgcca agggctggtc acccctcaac accaacatgt tcggcccccc cgaccaatgg
      181 ggatcctcgc ccaacgtgga tccggagacc cgcaagcccg cctgcgaatt caagtacgtg
      241 acggtctggg ccagccagga caagggccgg gtggcggtat tggccctggt ctatttcgac
      301 ccctcggcca acggggcctg cgcggccacg cccaaggtcc ggcgcctgcg ggtgaccctg
      361 gaagagttgc ccatgcccaa ggggtaggtt ttttccccgg aggaagccct tcgtccccgg
      421 tggggcgggg gctatactgg ggccccggat cggatccggg cattcggttc atcgggaggc
      481 gcggatgtcc aaggcttttt gggctggggt ggggacggcg gtcgtggcgg gactggtggt
      541 gggctacctt tcggtcatcc tgggcctgat gccggccaac gccgattcca agccgggcaa
      601 gttggaaacc tggatggcgc accattcgct gcgggccacc ctgcgccggg aggcgcctcc
      661 cggcgacaac cccctgcccg cggatgccgc ggacctgaag gccggcctgc gcctctacag
      721 cctcaattgc gcggtctgcc acggcgcctc cgacgcccgg gcgtcccaca tcgccatggg
      781 cctctaccaa cacgcgcccc aactggccca gcacggggtg gaggatgacc cggacggcgt
      841 catctattgg aagatcgccc acggcatccg gctcacgggg atgccgtcct tcgacggggc
      901 cctgaccgag aagcagcttt ggcagctcac ccttttcctc aagcacatgg acgccttgcc
      961 ccccgcggtg caaaaaagct ggaaggccct gcccagcgcc gccgtgaagg actgaggacc
     1021 gccggtcctt cgggcctgag cgggacttga caattattag aatattagta ttatctaatt
     1081 atgaaattgc ccgcccccca gatcgaaaag tccgccggat gcatcaaggc catggcccat
     1141 ccgctccggc tctccatcct ggccctcctc caagagggcg agaagaacgt ccaagacctc
     1201 acgcaggccc tgggggcagg gcaatccaat gtgtcccagc acctggccca gatgcgcgcc
     1261 cggggtttgg tgacggcgcg ccgggagggg aacatggtct attacgccgt ggggcatccc
     1321 aagatcgccc ggctgatggc cctgatgcgg gaggtctttt gcggggagtg attttttttg
     1381 cggtggaata ttagagatcg ctaataaagt cgggagggat ccatgcggac gctggcggct
     1441 gtggccctgg gggccttgtt gggattggct tggtggaggt tcgtgggatg ttccagcggg
     1501 gcatgcccca tcacctccaa cccctgggcc agcgccctct acggcgcggg gttggggttc
     1561 ctgatgagcc gcccgtgagc ggatatgacg gcggtcattc acggccgccg ggccgggggt
     1621 taagttgggg atggaaaatg aaaatgggga ggggaagacc atgaagaacg tcggaaggta
     1681 tgaccgggag gtccgcttca tcgcggccgc cctgctggcg gtgttggggt acctggaatc
     1741 ggggacggcc cggctgatcc tttgggtctt gatgctggtg ccgcttttca cggggctttt
     1801 ccgctattgc ccgctctgga cggtgttcaa gatcgacacc taccgggaaa agaggggccg
     1861 atgagcccct tttcactgat ggggcttttt gcggggaggc ccgacccggc ctcgctcgac
     1921 ccccaacagg tccgggaaag gctggcggcg gacccgaaga gccagttggt ggacgtgcgc
     1981 tcccacgagg agcggcggga gggttatttg aaggggtcca agcccatccc cctgccggag
     2041 ctttcgggac ggttgaagga attggacccg aaccggccgg tactccttta ttgccggagc
//
LOCUS       DASTJJ010000105         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_627578, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000105 DASTJJ010000000
VERSION     DASTJJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627578"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /gene="recQ"
                     /locus_tag="VFR02_01335"
     CDS             complement(<1..1420)
                     /gene="recQ"
                     /locus_tag="VFR02_01335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HET9869126.1"
                     /translation="MKPTGETPLSLLNRVFGYPAFRGRQEAVIEHLLAGGSCLVLMPT
                     GGGKSLCYQIPGMLRPGLALVVSPLIALMQDQVDALTQNGVAAAFYNSTLDGPAKDAI
                     RRKARQGGLDLLYVAPETLNSPGFQEFVRDLPLGLIAVDEAHCVSQWGHDFRPDYLQI
                     IRLRGIFPQVPLVALTATADPQTQKEIRERLGLAGDPVFSSSFDRPNLRYQITVKDGG
                     RDQLLDFIQTKHPGQSGIVYVLSRANTEETAAWLSQRGVEALPYHAGLDPALRRRHQE
                     RFLREEGLVMTATIAFGMGIDKPNVRFVAHLGLPKSVESYYQETGRAGRDGEPASAWM
                     AYSLDDVVKLRRMILGGGGDGSHKRLGGQKLNAMLGLCETVECRRRTLLAYFGEEHPG
                     GCGACDNCLAPPKTWDATLAAQKALSCVYRTGQRFGAGHLIDVLLGKGTDKAIQAGHT
                     QLGVFGVGKEYDKRRWDSVFRQL"
BASE COUNT          261 a          484 c          566 g          288 t
ORIGIN      
        1 ccaattggcg gaagaccgaa tcccagcggc gcttgtcgta ttccttcccc acgccgaaga
       61 cgcccagttg ggtgtgcccg gcttgaatgg ccttgtcggt ccccttgccc aagagcacgt
      121 cgatgagatg gcccgcgccg aaacgctggc ccgtgcggta gacgcaggac agggcctttt
      181 gggcggccag ggtggcgtcc caggtcttgg ggggcgccag gcaattgtcg caggcgccgc
      241 agccgcccgg atgttcctcg ccgaaatagg ccaagagggt ccggcggcgg cactcgacgg
      301 tctcgcacaa tcccagcatg gcgttgagct tttggccgcc caggcgcttg tgggacccgt
      361 cgcccccgcc gcccaggatc atgcggcgaa gcttcaccac gtcgtccagc gaataggcca
      421 tccaggccga ggcgggttcg ccgtcgcggc ccgcccggcc cgtctcctga tagtaggact
      481 ccacgctctt tgggaggccc aggtgggcca cgaagcgtac gttgggtttg tcgatgccca
      541 tgccgaaggc gatggtggcc gtcatgacca ggccctcctc gcgcaggaag cgttcctggt
      601 ggcggcgccg cagggccggg tccagccccg cgtggtaggg gagcgcctcc acgccccgct
      661 gggaaagcca ggcggcggtc tcttcggtgt tggcgcggct gagcacgtac acgatccccg
      721 actggccggg atgtttggtt tggatgaagt cgagcagttg gtcccggccc ccgtccttga
      781 cggtgatctg gtagcgcagg ttgggccggt cgaagctgga ggaaaagacc gggtccccgg
      841 cgaggcccag cctttcccgg atctctttct gggtctgggg gtcggcggtg gcggtgaggg
      901 ccaccagggg gacctgggga aagatcccgc gcaggcggat gatctgcagg tagtcgggcc
      961 ggaagtcgtg gccccactgg gaaacgcaat gggcctcgtc cacggcgatg agccccaggg
     1021 ggaggtcgcg gacgaattcc tggaagcccg ggctgttgag ggtctcgggc gccacgtaga
     1081 gcaggtcgag cccgccctgc cgggccttgc ggcggatggc gtccttggcg gggccgtcca
     1141 gggtggaatt gtagaaggcc gcggccacgc cgttctgcgt cagggcatcc acctggtcct
     1201 gcatgagggc gatgaggggc gaaaccacca gggccaggcc ggggcgcagc atgcccggga
     1261 tctggtaaca aaggctcttg cccccgccgg tgggcatcag caccaggcag gatccgcccg
     1321 ccaagaggtg ttcaatgacc gcttcctgcc gcccgcggaa ggccgggtag ccgaagacgc
     1381 ggttcaagag ggaaagcggc gtttccccgg tgggtttcat cgggcctcct ttgggatttg
     1441 gaacagatca atgcgtaagg agaagtctgt ttttaacaat gacgaaggct gatcgaactt
     1501 gatgttgata aggaaaaagc atcgggtttt atcagcgttc atcagggttc atcatcgcaa
     1561 atgtgccttt gcctgtcttt ccccagctgt tcctgttcg
//
LOCUS       DASTJJ010000106         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_995402, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000106 DASTJJ010000000
VERSION     DASTJJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995402"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFR02_01340"
     CDS             complement(<1..334)
                     /locus_tag="VFR02_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869127.1"
                     /translation="MSRSPDPGGIPPEITFIALPEALAQFHHLPPHVPLWVHREKDAK
                     AKGYDFEEGAHVMEELLRAAPRVPGAYLFQLFVKKWGKLKDVQSLIQARRVAEAIPPL
                     VEILELDPE"
     gene            485..1414
                     /locus_tag="VFR02_01345"
     CDS             485..1414
                     /locus_tag="VFR02_01345"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9869128.1"
                     /translation="MLDSLSDALGWLHPPATHFPIVCSILAFLAFAFSRGRDWLQKSA
                     AALWILAFLTGVPSVLLGHLFAHHLGLVSTWSWLPPASALKGQLRFHALLGSLGLLLS
                     AATLWGAGRLARGKPVSFALQLTLGLFTAALFGAAGHEGGEMVYGGDEAPAVSTATPP
                     SPATAASTSSLTERAKSVLPSLVKMNTRPWVSKTHGGRWVDTYVSRDAVKAYQDSDPL
                     PPGTWVVKESFEDAGGKPSTVKGPLYVMRKGPQKWDYAMEWDKPVPGNPEKITGPVTW
                     LPGNAALDSCVKCHSHFHNVDYMGGVPTEAAVP"
     gene            complement(1483..>2620)
                     /locus_tag="VFR02_01350"
     CDS             complement(1483..>2620)
                     /locus_tag="VFR02_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9869129.1"
                     /translation="AFTYGGRPAGLLIARDITQRKKAVQAFRDSQESYRRLVEFFPET
                     VLVHSEGKLLYINPPGLKMFGASDPAEILGRSVYDFVATPFLETARIRVESIYLNKLS
                     TDPMDQVWLKVDGTPIDVEIRGTFFNFMGKPSILSILRNVSERKRTQQMLLRYERLAA
                     VGQVIAGIAHDIRNPLAVLSSMTHHLREKFAGLDGPGSREVEVIASQTERLKRLMNDI
                     LDYAKGLTLVKQPIAPAPFLEQCLRLVQAQVGPAHSRVAVQWDLKGASRPFPGDRERL
                     EQVFLNLMLNAYEAMEPGGTLTLSFESGGGWARLKVADDGPGIPEAALARLFEPFFTT
                     KKYGSGLGLSVSQKIVEAHGGRIEAEWVYPHGTLFTVTLPGETA"
BASE COUNT          418 a          849 c          890 g          463 t
ORIGIN      
        1 attcggggtc cagctccagg atctccacca ggggcgggat ggcctcggcc acccggcggg
       61 cctggatgag ggactgcacg tccttgagtt tgccccactt cttgacgaag agctggaaca
      121 ggtaggcccc gggcacccgg ggggcggccc gcaacagttc ctccatcacg tgggcgcctt
      181 cctcgaaatc gtaacccttg gccttggcgt ccttttcccg gtgcacccaa aggggcacgt
      241 gcgggggcag gtggtggaac tgggccaggg cctccgggag ggcgatgaag gtgatttcgg
      301 gcgggatgcc gccggggtca ggggatcggc tcatggcttt cctttcatgg gtcggtttgc
      361 attataagaa tccggggatg gaattccacg agcacccagg aattccgggc ggaaaatccc
      421 gtaacccggg gccggggccg atgctattct ggaaggacgg tcactcccct tataaggaaa
      481 gctcatgctc gactccctgt cggacgccct gggctggctg catccgcccg ccacccactt
      541 ccccatcgtt tgctccattt tggccttcct ggccttcgcc ttcagccgcg gccgggactg
      601 gctccaaaaa agcgccgccg ccctctggat cctggccttc ctgaccgggg tcccgagcgt
      661 actcttgggc catcttttcg cccaccacct ggggctggtc tctacttggt cctggcttcc
      721 gcccgcttcg gccctgaaag ggcagctgcg gttccacgcc cttttgggca gcttgggcct
      781 gctcttgtcg gcggccaccc tctggggcgc cgggcgcttg gcccggggaa aaccggtgtc
      841 gttcgccctg caactcaccc tggggctctt tacggccgcc ctcttcggcg cggcgggcca
      901 cgagggcggc gaaatggtct atgggggcga tgaggcgccg gccgtttcca cggcgacgcc
      961 cccctccccg gcgacggcgg cttccacttc cagcttaacg gagcgggcca agtcggtctt
     1021 gccctccctg gtgaagatga acaccagacc ctgggtgagc aagacccacg gcggccggtg
     1081 ggtggatacc tatgtctcga gggacgcggt gaaggcctac caggattcgg accccctgcc
     1141 gccggggacc tgggtggtga aggaatcctt cgaggacgcg ggtggaaagc cctccacggt
     1201 gaagggcccc ctttacgtca tgcgcaaggg cccccagaag tgggactacg ccatggagtg
     1261 ggataagccg gtgccgggca accccgaaaa gatcacgggg cccgtcacct ggctgccggg
     1321 gaacgcggcg ctcgattcct gcgtgaaatg ccactcccat ttccacaatg tggactacat
     1381 gggcggcgtg ccgacagagg ccgccgtgcc atgagcggcg cgtcatcaga ccgccgccca
     1441 tcccacggcg tgccggggga cgtggccgtt ccctgaaggg gtttaggccg tttccccggg
     1501 cagggtcacg gtgaaaagcg tgccgtgggg atagacccac tcggcctcga tccggccgcc
     1561 gtgggcttcc acgatcttct ggctgaccga caatcccagg cccgatccgt acttcttggt
     1621 ggtgaagaag ggctcgaaga gccgcgccaa ggcggcctcc gggatgcccg gaccgtcgtc
     1681 ggcgaccttg agccgcgccc atcccccccc ggactcgaag gaaagcgtga gcgtgccgcc
     1741 cggttccatg gcctcataag cgttcaacat caaattgagg aagacctgct ccagccgctc
     1801 ccggtccccg gggaaaggcc gggaggcgcc cttcaggtcc cattgcaccg ccacccggga
     1861 atgggccggc cccacctggg cctgcaccag ccggaggcat tgttcgagga agggcgccgg
     1921 ggcgatgggc tgcttcacca gggtcaaccc cttggcgtag tccaggatgt cgttcatgag
     1981 ccgcttcagg cgctcggtct gggacgcgat gacctccacc tcccgggatc ccgggccgtc
     2041 caagccggcg aacttttccc gcaggtggtg ggtcatggag gacagcacgg ccagggggtt
     2101 gcggatgtcg tgggcgatgc cggcgatgac ctggcccacg gccgccaggc gctcgtagcg
     2161 cagcagcatc tgctgggtgc gcttgcgctc ggacacgttg cgcaggatgg acaggatgga
     2221 gggtttgccc atgaagttga agaaggtgcc ccggatctcc acgtcgatgg gcgtgccgtc
     2281 caccttgagc cagacctggt ccatggggtc ggtggacagc ttgttgaggt agatgctctc
     2341 cacccggatg cgcgcggtct ccaggaaagg cgtggccacg aaatcataga cggaccggcc
     2401 caggatctcg gcggggtcgg aggcgccgaa catcttgagg cccggcgggt tgatgtacag
     2461 gagcttgccc tcgctgtgca ccaggacggt ctcggggaag aattcgacca aacggcggta
     2521 gctttcctgg ctgtcccgga aggcctggac ggcctttttg cgctgggtga tgtcccgggc
     2581 gatcaggagc cccgcgggac gccccccata ggtgaaggcc
//
LOCUS       DASTJJ010000107         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_873180, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000107 DASTJJ010000000
VERSION     DASTJJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873180"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..>1527
                     /locus_tag="VFR02_01355"
     CDS             220..>1527
                     /locus_tag="VFR02_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET9869130.1"
                     /translation="MRNLLFFLLLPLLAPIRSFSQAARKPDIIIILADDLGWGDVGFH
                     GSDIRTPHIDQLAREGVVLNYFYTAPICSPSRAGLMTGRYPNRFGLRQNVIPPWSDFG
                     VDTSEVFLPQLLARAGYKNRAALGKWHLGDAYKRYLPLHRGFTHFYGFYNGALDYFTH
                     LREGQLDWHNDSATCRDTGYTTDLITREAVRCIRQYAKDPSPFFLYVAYNAPHGPLQA
                     KRGDLLSYGFDPAKPLFPHKGAQGQEGRGNTRRQTYSAMVTCLDSGVGTILQTLADLH
                     LKNNTLVLFFSDNGAAPGGGGSSGELKGWKFQEWAGGVRAPAIVRWPEGMKEPRVTNQ
                     VTGYVDVLPTLLAAAGLKARSGKPLDGINMLSVWSGRRDSIDRYFYLGHGALIHSPWK
                     VVKAHAGNPRMKEPADVLTNILTDPDEKHNLAAAHPDILRQLLG"
BASE COUNT          321 a          482 c          443 g          281 t
ORIGIN      
        1 gcgatcctta cgaatggaag aacgtggtcg ccgaacccgg catgcaggac gtgatcaaaa
       61 ggctgagcgc ctcgatccct tcccatttta ccccctctat ccccccgggt catgccaaaa
      121 agaaacatcc ggggaagaag ccgggagcac caggcgtgca tcaccagaaa aaaggcgccc
      181 gtcatcccat caaacagcca tccctttaaa ccaattacca tgcgaaacct tctttttttc
      241 cttctcctgc cgctgttggc tccgatccga agcttttccc aggccgcgcg taagccggac
      301 atcatcatca tcctggccga cgacctgggc tggggcgatg taggttttca cggcagcgat
      361 atccgcacgc cccacattga ccagctggcc cgggaaggtg tggtcctgaa ctacttttat
      421 actgcgccga tctgctctcc cagccgggcc gggctgatga cggggcgcta ccccaaccgt
      481 ttcggactgc ggcagaatgt catcccgccc tggagtgatt tcggggtgga tacttccgaa
      541 gttttcctgc cgcagctgct ggcacgggca ggctataaaa accgcgcggc actgggcaag
      601 tggcacctgg gagacgcgta caagcggtat ttgccgctac acaggggctt cacccatttt
      661 tacggcttct acaacggtgc gctggattat tttacccacc tgcgtgaggg ccagcttgac
      721 tggcacaatg attccgccac ctgccgcgac acgggataca ccaccgacct gatcacgagg
      781 gaagcagtga ggtgcatccg gcagtacgcg aaagatcctt cgccgttttt cctttacgta
      841 gcttacaatg cgccgcacgg tcccctgcag gcgaagcggg gggacctgct gagctacggg
      901 tttgatcccg ccaaacccct ctttccccac aagggagccc agggacagga aggacggggc
      961 aataccaggc ggcaaaccta ttccgccatg gtcacctgcc tggacagcgg cgtgggcacc
     1021 atcctgcaga ccctcgctga cctgcacctc aaaaacaaca ccctcgtcct attcttcagc
     1081 gacaacggcg ccgcaccggg agggggtgga tccagcggag aactaaaggg ctggaagttc
     1141 caggaatggg ccggcggggt aagggctccg gccatcgtcc gctggccgga aggcatgaag
     1201 gagccccggg tgacgaacca ggtcaccggg tatgtggatg tgctccccac cctgctggct
     1261 gccgccgggc tgaaggcgcg gtcgggaaag ccgctggacg gcatcaatat gctgtcggta
     1321 tggagcggcc gcagggacag catagaccgg tatttttacc tcggccacgg cgccctcatc
     1381 cacagtccct ggaaggtcgt gaaggcacat gccggcaacc cccgcatgaa agagcctgcc
     1441 gacgtgctga ccaatatttt gacggatccg gatgaaaagc ataacctggc cgctgctcac
     1501 ccggacatcc tgcggcagct gctgggg
//
LOCUS       DASTJJ010000108         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_506286, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000108 DASTJJ010000000
VERSION     DASTJJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506286"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1136
                     /locus_tag="VFR02_01360"
     CDS             168..1136
                     /locus_tag="VFR02_01360"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HET9869131.1"
                     /translation="MRTPSYKIPSLLLALLLLDAARALAGAPAAGKFQAPAFLLQWGG
                     WGSDPGRMWNPYGLAVGPEGSVYVDDYVNERVEVFDPSGKYLRQWGRRGTAPGRFHHA
                     LGLAVGPSGDVYVADTLNDRVQKFTADGVWLAQWGGDGAGPGQFFNPVMATVGKDGRV
                     LVVDGSNGRVEAFSPEGKFLFQFGGPGSGQGRFNLPTGVAVGPDGSLYVTDTGNHRVE
                     KFDAQGVFLTQWGSKGKAPGQFQGPVGLAVDPQDRVYVADSGNGRVQVFTASGRFLTQ
                     WGSPGKGQGQFTQPEGVALDAQGDVYVADWGNCRVEKFSPLSGGNP"
     gene            1133..>2041
                     /locus_tag="VFR02_01365"
     CDS             1133..>2041
                     /locus_tag="VFR02_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HET9869132.1"
                     /translation="MNGRIPDRGLLERIARQAMAENGLEPDFPPQALEQLAGIPGPDR
                     DPSLPDLTGLPWCSIDNDDSRDLDQLSAAQRLPNGGIRVLVAVADVDGRVSKNTPLDL
                     HAQKNTTTVYTGVATFPMLPERLSTDLTSLSPNEERTALVVEMTLDGEGRVGASRILR
                     ARVLNRAQLAYNGVGAWLEGRGPLPEAAARVPGMEAQLRLQDEAAQRLVARRHEHGAL
                     ALQTLQPRPVTQDGLVVDLALEVQNRAGQLIEDFMVAANGATARFLTAQGFPTLRRVV
                     RKPKRWDRIVSVAASLGETLPGDPDPK"
BASE COUNT          337 a          692 c          705 g          307 t
ORIGIN      
        1 tttccaggcg aggaaaaagg gcgaacgccc gaggagaccg tcgatgagcg atccgaaatg
       61 gccggccaat tgtcgggtgg ggtccatagg gtccttgggg ctccttcaaa accgcccgga
      121 ggcaagtgta caatgaaatg cttcaaatcc atccagggcc ggtgccgatg aggaccccat
      181 cctacaagat cccaagcctt ctcctggccc tcctgctttt ggacgccgcc cgggccctgg
      241 ccggggcccc ggcggccggg aaattccaag ccccggcctt ccttctccaa tggggcggct
      301 gggggtccga cccggggcga atgtggaatc cctacgggct ggcggtgggg cccgagggtt
      361 cggtctacgt ggacgactac gtcaacgagc gggtggaggt ctttgacccc tcgggcaagt
      421 acctgcgcca atggggccgc aggggcacgg cccccgggcg gttccaccac gccttggggc
      481 tcgcggtggg cccctccggc gacgtctacg tggccgatac cctgaacgac cgggtgcaaa
      541 aattcacggc cgacggcgtc tggttggccc aatggggcgg cgacggcgcc ggaccggggc
      601 agttcttcaa ccccgtaatg gcaacggtgg gcaaggacgg ccgggtcctg gtggtggacg
      661 gcagcaacgg ccgggtggag gctttttccc cggaaggcaa gttcctcttc caatttggcg
      721 gccccggatc cggacagggg cgtttcaacc tgcccaccgg cgtggccgtg gggcccgacg
      781 gttcccttta cgtcaccgac acggggaacc accgggtgga aaagttcgac gcccaggggg
      841 tcttcctcac ccaatggggg tcaaagggaa aggccccggg ccagttccag gggccggtcg
      901 gcctggcggt ggacccccaa gaccgggtct acgtggcgga ttcgggcaac ggccgggtgc
      961 aggtcttcac ggcgtcgggc cgtttcctca cccaatgggg atcgcccggc aagggccagg
     1021 ggcaattcac ccagcccgag ggcgtcgcgc tggacgccca gggggacgtc tacgtggccg
     1081 actggggcaa ctgccgggtg gaaaaattca gcccgctttc cggaggcaac ccatgaacgg
     1141 acggatcccc gaccgggggc ttctggagcg gattgcccgg caggccatgg ctgagaacgg
     1201 tttggagccg gatttcccgc cccaagccct ggaacaactg gcgggcatcc ccggccccga
     1261 ccgcgaccct tccctgcccg acttaacggg cctgccctgg tgttccatcg acaacgacga
     1321 ctcccgggac ctggaccagc tcagcgcggc ccaaaggctc cccaacggcg gcatccgggt
     1381 gctggtggcg gtggccgacg tggacggacg ggtatcgaaa aacacgccct tggacctgca
     1441 cgcgcaaaag aacaccacga cggtctacac cggcgtggcg actttcccca tgctgcccga
     1501 gcgcctctcc accgacctga cctccctttc ccccaatgag gaacgcacgg ccctggtggt
     1561 cgagatgacc ctggacgggg aaggcagggt cggggcatcc cggatcctcc gggcccgggt
     1621 gctcaaccgg gcccaactgg cctataacgg cgtgggcgcc tggctggagg gccgcggccc
     1681 cttgccggaa gccgccgcgc gggtgcccgg catggaggcc caattgcgcc tgcaggacga
     1741 ggccgcccaa cgcctggtcg cgcggcgcca cgaacatggc gccctggccc tgcagaccct
     1801 gcagccccgg cccgtcaccc aggacggcct ggtggtggac ctggcgctgg aggtccagaa
     1861 ccgggccgga cagttgatcg aggatttcat ggtggcggcc aacggcgcca ccgcgcgttt
     1921 tttgacggcc cagggcttcc ccaccctgcg ccgggtcgtg cgcaagccca agcgctggga
     1981 ccggatcgtt tcggtggccg cttccttggg cgaaaccttg cccggcgatc ccgaccccaa
     2041 g
//
LOCUS       DASTJJ010000109         1482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_384258, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000109 DASTJJ010000000
VERSION     DASTJJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384258"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..>1482
                     /locus_tag="VFR02_01370"
     CDS             253..>1482
                     /locus_tag="VFR02_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9869133.1"
                     /translation="MERFEGLNFILRSARWAAWALLFCLLAAADASAANKDYCVHVDY
                     SAVPSLVNDRKLTLLVNVGNCASVSVTADGSPVPATYNSSTGMALFTTTGSALVVTAV
                     NWTSGGTGAATKATLYNNYHWAYSLTFDDNRQTQYTNGKPVLDAHGWRAAVAVVGSWA
                     NAGGYWMTWTTLQSLRAAGWDILNHSYDHPNPITCANLTNELSQNQTLLQTYFPSYHV
                     SQFVFPYENSSASTCGGFPPSYLISSECGGGSYNYVDNPLAFQAQRGGLFGTDDTAWK
                     SLAASAAGNSRPTWLVEYTHSVAAGSAAAEDAYSTNAATLGDLLNYLDNNYGASGNKS
                     LWFAPAGEVRDYLFTRDDSVVTTCAVVTPTPTLTPSRTPTPAATATGTATKTASPTAS
                     MTRTPTATLSPTPTPSLT"
BASE COUNT          252 a          530 c          434 g          266 t
ORIGIN      
        1 ccgcttcgcc gacgccgcga aggcgggccg tgaggccgag gccgctgagg atttccaacc
       61 ttaaagtccc ggcctggacg acctttccgg cttgaaggct tgtttgtttt cccggcaccg
      121 atttaacggg caacccgcgc cgtctttttc ccttgaaata aaaggcaaat cttttgaccg
      181 gtaagggcta acccttttgg gggtggggcg gggaaaccgg cgttttgggc ttatgctttt
      241 tccatgttcc ccgtggaaag gtttgaaggc ctgaatttca tcctccggtc cgcccgttgg
      301 gcggcttggg ccctcctgtt ttgcctcctg gcggcggccg atgcttccgc cgccaacaag
      361 gactattgcg ttcacgtgga ctattcggcc gtgccgtccc tggtgaacga ccgaaagctc
      421 accctcttgg tcaacgtggg gaactgcgct tcggtgtcgg tcacggccga cggttcgccg
      481 gtcccggcca cctataattc ctccacgggc atggccctct tcaccacgac gggttccgcc
      541 ctcgtggtca ctgccgtcaa ttggacctcg ggcggcacgg gcgccgccac caaggccacc
      601 ctctacaaca actaccattg ggcctattcg ctcaccttcg acgacaaccg gcagacccaa
      661 tacaccaacg gcaagccggt cctggacgcg catgggtggc gggcggccgt ggccgtggtg
      721 gggtcctggg ccaacgcggg gggctactgg atgacctgga cgacccttca atccctgcgc
      781 gcggcgggct gggacatcct caaccattcc tacgaccatc ccaaccccat cacctgcgcc
      841 aacctgacca acgagctcag ccagaaccag accctgctgc agacctattt cccgagctac
      901 cacgtgagcc agttcgtctt cccctacgag aattcctcgg ccagtacttg cggtgggttc
      961 ccgccttcct acctgatctc ctcggagtgc gggggagggt cctacaacta cgtggacaac
     1021 cccctggcct tccaggccca acgcgggggg ctcttcggca ccgacgatac ggcctggaaa
     1081 tcgctggccg cctcggcggc cgggaacagc cggccgacct ggttggtgga atacacccac
     1141 agcgtggcgg cggggagcgc ggccgccgag gacgcctaca gcaccaacgc ggccaccctg
     1201 ggcgacctgc tcaattacct ggacaacaat tatggggcct cggggaacaa gagcctgtgg
     1261 ttcgctcccg cgggggaagt gcgggattac ctcttcacgc gggatgattc ggtggtgacc
     1321 acctgcgcgg tcgtgacccc cacgccgacc ctgacgccca gccgcacccc caccccggcg
     1381 gccacggcca ccgggacggc caccaagacc gcgagcccga cggccagcat gacccgcacg
     1441 cccaccgcga cgctttcccc cacgcccacc ccgtccctca cc
//
LOCUS       DASTJJ010000110         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_506395, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000110 DASTJJ010000000
VERSION     DASTJJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506395"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFR02_01375"
     CDS             complement(<1..397)
                     /locus_tag="VFR02_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869134.1"
                     /translation="MKNKKPSPKPTPLLPRVLLGAALLALAGFLYFVYGYLPAQVGKA
                     LTERAKVRQPLTTTPKADGLAYQDAAFTAADGVPLKGWWLPSAAPGKARGTVILTHGV
                     FKNREQVLARAEFLARAGYQVLLFDLRGCG"
     gene            complement(394..1278)
                     /gene="ispE"
                     /locus_tag="VFR02_01380"
     CDS             complement(394..1278)
                     /gene="ispE"
                     /locus_tag="VFR02_01380"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET9869135.1"
                     /translation="MSAPALKVKTPAKVNWALEVLRRRDDGFHELALVFQAVGLWDEL
                     SFWKKASGLELAVLESPVPLGTDPSNLVLRAARLFFEAAGLPEGLRVELTKRIPVAAG
                     LGGGSSDAAATLWALNRLYGTDFSLAQLRHLGAQLGSDVPFFLTGGTALGLGRGEQVT
                     PWDPAPTRWLVLVKPQEGLSTPAVYGSGRAAFSTGEKALGMKAALTGGNGPLLAASLF
                     NSLEPAAFHLLPEVEALKNGLLRAGALGALVSGSGPTVFGLAADEAAARAVAEKMRTL
                     APTVWVVPTVAEGPRIVP"
     gene            complement(1275..>2978)
                     /locus_tag="VFR02_01385"
     CDS             complement(1275..>2978)
                     /locus_tag="VFR02_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869136.1"
                     /translation="LHYCLCLLALLAPAVLRADTFEPDESALEQQADAYWHYACGLSA
                     LLRGDQDRSLAEFEQSARLDPGSKAVHDQLAFHYYQRGLDDQSQDELRKAIELDPADV
                     EDRLLLAGQLAGKGAYGQAQDQYQRVLQSDPKNLEARYYLAGVLAAQDRYDDALRGYR
                     DILKDNPKSAGIYYNIGLLYVRQNKSAEAESSFQQAILLDPGLAPAYQSLGLVYELDQ
                     KPDRALATYAALVKVKPNDAQAYEAMGELYYNANEDEKALDAFRQYAKLKPDDPAIQD
                     YIGVCCYQLKDYASAIQAFQVLLAAQPANDLVRYRLAATYVEAGQEDAALSQLNTLTA
                     HDPKNTDAWYQLTLIYHNQKKADLTQKALAAGLKANPDSEDLVLLKAELDGEKGDPAA
                     AEAGYRRVLQMDPKNVRACFDLGAMLDQQGRFDEAMDLMKKVVQLQPDNAEAFNYIGY
                     AYADKDRNLDEAQRDVERALKLDPDNAYYLDSLGWVLYRKNQLDTAKEKFEMAIRQLA
                     PADKLNKDDAVIYDHLAHVLVKMGKKDEARAQLQKALELDPTNKTYLSDQTKLEASDS
                     L"
BASE COUNT          437 a          959 c         1059 g          523 t
ORIGIN      
        1 ccccgcagcc ccgcaggtcg aacaagagca cctggtaacc cgcgcgggcc aggaactcgg
       61 cccgggccag cacctgttcg cggttcttga agacgccgtg ggtcaggatc accgtgcccc
      121 gcgccttccc gggcgcggcc gagggcagcc accagccctt cagggggacg ccgtcggcgg
      181 ccgtgaaggc ggcgtcctgg taggcgaggc cgtcggcctt tggggtggtg gtgaggggct
      241 ggcgcacctt ggcccgctca gtcagggcct tccccacctg ggcgggcagg tagccgtaga
      301 caaaataaag gaagccggcc agggccaaga gggccgcccc caggaggacg cggggcagca
      361 gcggcgtggg cttggggctt ggtttcttgt ttttcatggg acgatcctgg gaccttcggc
      421 cacggtgggg accacccaca ccgtgggcgc gagggttctc attttttcgg ccaccgcgcg
      481 ggccgcggcc tcgtcggccg ccaggccgaa caccgtgggc ccgctgcccg acaccaacgc
      541 gcccagggcc cccgcccgca gcaggccatt cttaagggcc tccacctcgg ggagcaagtg
      601 aaaagcggcg ggctccagcg aattgaagag cgacgccgcc aaaagaggtc cgttgccgcc
      661 ggtgagcgcc gccttcatcc ccagcgcctt ttcccccgtg gagaaggccg ccctgcccga
      721 tccgtacacg gcgggcgtgg aaagcccttc ctggggcttc accaacacca gccagcgcgt
      781 gggcgcgggg tcccagggcg tcacctgctc gccccgcccc aaccccagcg ccgtgccgcc
      841 ggtgaggaaa aagggcacgt cggaccccaa ttgggccccc aggtggcgca attgggccag
      901 ggaaaagtcg gtcccataga gtcggttgag ggcccagagg gtggcggccg cgtcgctgga
      961 cccgccgccc aggcccgcgg ccaccgggat gcgcttggtc agttcgaccc tgaggccctc
     1021 gggcaggccc gcggcctcga aaaaaagccg ggccgcccgc agcaccaggt tggaggggtc
     1081 ggtgcccaag gggacaggcg attccaggac ggccagttcc agccccgagg cctttttcca
     1141 gaaggacagt tcgtcccaca ggcccacggc ctggaagacc agggccaatt cgtggaaacc
     1201 gtcgtcgcgc cggcgcagca cttccagcgc ccaattgacc ttcgcggggg ttttaacctt
     1261 cagggcgggg gcgctcacaa ggagtcgctg gcctccagtt tggtctggtc gctgaggtac
     1321 gtcttgttgg tcgggtccag ttccagggcc ttctggagct gggcgcgggc ctcgtccttc
     1381 ttgcccatct tcaccagcac gtgggccagg tggtcgtaga tgacggcgtc gtccttgttg
     1441 agcttgtcgg cgggcgccaa ctggcggatg gccatctcga acttttcctt ggcggtgtcc
     1501 aactggttct tgcggtaaag cacccagccc aggctgtcca ggtagtaggc gttgtccggg
     1561 tccagcttga gggcccgctc cacgtcccgc tgggcctcgt ccaggttccg gtccttgtcc
     1621 gcgtaggcgt agccgatgta gttgaaggcc tcggcgttgt cgggctgcag ttggacgacc
     1681 ttcttcatca ggtccatggc ctcgtcgaac cggccctgtt ggtccagcat ggcccccagg
     1741 tcgaagcagg cccggacgtt cttggggtcc atctgcagca cccgccggta gcccgcctcg
     1801 gcggccgccg gatcgccctt ttcgccgtcc agttcggcct tcaacagcac caggtcctcg
     1861 ctgtccgggt tggccttgag cccggcggcc agggcctttt gggtcaggtc ggccttcttt
     1921 tggttgtggt agatcaaggt gagctggtac caggcgtcgg tgttcttggg atcgtgggcc
     1981 gtcagggtgt tcagttggga cagggccgcg tcctcctgcc cggcctccac gtaggtggcg
     2041 gccaaccggt aacgcaccag gtcattggcg ggctgggcgg ccagcaggac ctggaaggcc
     2101 tggatggccg acgcgtaatc cttgagctgg tagcagcaga ccccgatgta gtcctggatg
     2161 gcggggtcgt cgggcttgag cttggcgtac tgccggaagg cgtccagggc cttttcgtcc
     2221 tcgttggcgt tgtaataaag ctcccccatg gcctcatagg cctgggcgtc gttgggcttg
     2281 accttcacca gggccgcgta ggtggccagg gcccggtcgg gcttctggtc gagctcgtag
     2341 accaggccca aggactggta ggcgggcgcg agcccgggat cgagcaggat ggcctgttgg
     2401 aaggaggatt cggcctcggc ggacttgttc tggcgcacgt agagcaggcc gatgttgtag
     2461 tagatgccgg cggacttggg gttgtccttc aggatgtccc gatagcccct caaggcgtcg
     2521 tcgtagcggt cctgggcggc cagcacgccg gccaggtaat agcgggcctc caggttcttg
     2581 gggtcgctct gaaggacccg ctggtactgg tcttgggcct ggccgtaggc gcccttgccg
     2641 gccaactggc cggccagcag caggcggtcc tccacgtcgg cggggtcgag ctcgatggcc
     2701 ttgcgcagct catcctggct ttggtcgtcc aggccccgct ggtaatagtg gaaggccagt
     2761 tggtcgtgga cggccttgga gccggggtcc agccgggcgg actgctcgaa ctcggccagg
     2821 gaccggtcct ggtccccccg cagcagggcg gacaggccgc aggcgtagtg ccaataggcg
     2881 tcggcctgct gttccagggc ggactcgtcg ggctcgaagg tatcggcccg caggaccgcc
     2941 ggggccagga gcgccaagag gcaaaggcaa taatggag
//
LOCUS       DASTJJ010000111         1337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1729540, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000111 DASTJJ010000000
VERSION     DASTJJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729540"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..943)
                     /locus_tag="VFR02_01390"
     CDS             complement(188..943)
                     /locus_tag="VFR02_01390"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9869137.1"
                     /translation="MRWPRETLIQLYLEGGLRPEAQREFNRLMRKDPTFPNEVTEAAQ
                     RAFRSLPVGAPPAPVPAVPAPTAPPPVPVRAPLRPRFSWPAFEPKKLMPHLGLGLMVL
                     AALGVLFLAADWIRQARPRPKPRPAAAVPSDPIRQVLVKDQSPSPEEEGAIGVSPSLS
                     AAGKELVFQVGDRIYLSIQSARKQNVTLSICVPEGRVVRRLYSGEWQKGAQEITWDGL
                     DGAGRPTPPGAYLVVLKAGGKTMSDRLILEPAP"
     gene            complement(1010..>1337)
                     /locus_tag="VFR02_01395"
     CDS             complement(1010..>1337)
                     /locus_tag="VFR02_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HET9869138.1"
                     /translation="QGTFTNGQKVALALLALAQVNNPEGLASVGNGYFEATASSGGLQ
                     MGLAGTAGMGAVEGGVLENSNVDLTVELSNMIIAQRSFEANAREAAVVNSTLQTLAQL
                     GQVSAA"
BASE COUNT          230 a          464 c          445 g          198 t
ORIGIN      
        1 gggggccgcg cccctggccg ccctggcttc cctggcggcc gcccaatggt tctgggaccg
       61 gggattggcc caatatcaaa gcacgggttc ctgaccgtcc cgaaggcccc aagaaaaaag
      121 gccgcaagac ccatggggtc ttgcggcctt tttcgatcga ccaatcaggg cgcggggccc
      181 cgggccctca gggcgccggt tcgaggatca ggcggtccga catggtcttg ccgccggcct
      241 tcaggaccac caggtaggcc cccggcggcg tcggccgccc ggccccgtcc aacccgtccc
      301 aagtgatctc ctgggcgccc ttctgccatt cacccgaata gagccggcgc acgacccggc
      361 cctccgggac gcagatggag agggtgacgt tctgcttgcg ggccgactgg atcgaaagat
      421 agatccggtc ccccacttgg aagaccagtt ccttgccggc cgccgacaaa ctgggggaaa
      481 ccccgatggc gccctcttcc tcgggggacg gggactggtc cttcaccagg acctggcgga
      541 tggggtcgga agggaccgcc gcggccggcc ggggtttggg acgcggccgg gcctgccgga
      601 tccaatccgc cgccaggaac aacaccccca gcgccgccag gaccatcaat cccaacccca
      661 aatgcggcat cagcttttta ggctcgaagg ccggccagga gaaccggggc cgcagggggg
      721 cccggacggg gacggggggc ggggcggtcg gcgcggggac ggccggcacg ggtgcgggcg
      781 gggcgcccac cggaaggctg cggaaggcac gctgggccgc ttcggtcact tcattgggga
      841 aggtggggtc cttgcgcatc aagcggttga attcgcgctg cgcttccgga cgcaggccgc
      901 cttccagata gagttgaatg agggtctcac ggggccaacg catgaccgca ttataatcac
      961 gccaccgcga aagacggaac aacaacgtta aacccccaaa ccggcgggat caggccgcgg
     1021 agacctgccc taattgggcc agggtctgga gcgtgctgtt caccacggcc gcctcgcggg
     1081 cattggcctc gaagctgcgc tgggcgatga tcatattgga caattccacc gtcaggtcca
     1141 cgttggaatt ctccagcacc ccgccctcca ccgcacccat ccccgccgtg cccgccaagc
     1201 ccatctgcag gccccccgag ctggcggtcg cctcgaaata gccgttgccc accgacgcca
     1261 gaccctcggg gttgttcacc tgggccagcg ccaatagggc caaggccact ttctgtccgt
     1321 tggtgaaggt cccctgc
//
LOCUS       DASTJJ010000112         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_261820, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000112 DASTJJ010000000
VERSION     DASTJJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261820"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFR02_01400"
     CDS             <1..672
                     /locus_tag="VFR02_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9869139.1"
                     /translation="GRSLAVATGMKMANHGMTVLVTGGDGDGFGIGGNHFMHTMRRNV
                     DLTYIVMDNQIYGLTTGQTSPTSETGMKTKSTPNGNPEGQLNPMALALAAGCGFVARG
                     FSGDINHLKSLYTQAIQYPGFALIDVFSPCVTFNKQNTFGWFKERVYKLEEKDHDVTN
                     WNAAMERAKEWGAQIPIGVLYKNPNPKPSIDAADPALQGTPLARQTKLGLTKEQRAKL
                     IEEYV"
     gene            873..>1060
                     /locus_tag="VFR02_01405"
     CDS             873..>1060
                     /locus_tag="VFR02_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9869140.1"
                     /translation="MEEIYDISIVGGGPAGMFAAFYAGIRGMNFKIIDSLEALGGQVS
                     AMYPEKDIFDVAGFPRVAG"
BASE COUNT          217 a          349 c          320 g          174 t
ORIGIN      
        1 gggcgctcgc tggctgtggc cacaggcatg aagatggcca accatggcat gacggtgctg
       61 gtgacgggcg gcgatggcga cggcttcggc attggcggca accactttat gcacaccatg
      121 cgccgcaacg tcgacctgac ctacatcgtc atggacaacc agatctacgg cctcaccacc
      181 ggccagacct cgcccacctc cgagacgggc atgaagacca agtccacccc caacggcaac
      241 cccgaggggc agttgaaccc catggcgctg gccctggcgg ccggctgcgg tttcgtggcg
      301 cggggcttct cgggcgacat caaccacctg aagagcctct acacccaggc catccagtac
      361 ccgggcttcg ccctgatcga cgtcttcagc ccctgcgtga ccttcaacaa gcagaacacc
      421 ttcggctggt tcaaggaacg cgtgtacaag ctggaggaga aggaccacga cgtgaccaac
      481 tggaacgcgg ccatggaacg cgccaaggaa tggggcgccc agatccccat cggcgtgctc
      541 tacaagaacc cgaaccccaa gccttccatc gacgcggccg acccggccct gcagggcacg
      601 ccgctggcgc gccagacgaa gcttggcctc accaaggaac agcgcgcgaa gctcatcgag
      661 gagtacgtct agcaattttg tggggcgccg acagttttgg cgccctgatg gtcccggatg
      721 aaaagaaagg cccttccccc gcggaaaggg cctttccttt tttgtgcggt tttgattaaa
      781 taacgcggcg caaaaaacgt ttttatcgtc ccaggcccag gcggtttcgc cggcccccca
      841 acgacgagcg ttcaaaatcc aggagggcat tcatggaaga gatctacgac atcagcatcg
      901 tgggcggcgg gccggcgggc atgttcgccg ccttctacgc cggcatccgg ggcatgaact
      961 tcaagatcat cgactccctg gaggccctgg gcggccaggt gtcggccatg tatcccgaga
     1021 aggacatctt cgacgtggcc gggttccccc gcgtggccgg
//
LOCUS       DASTJJ010000113         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_384722, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000113 DASTJJ010000000
VERSION     DASTJJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384722"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFR02_01410"
     CDS             <1..1050
                     /locus_tag="VFR02_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET9869141.1"
                     /translation="DDSVSAPAHLADYGLDHPGVQAQFKFKDGSAQTLLVGDKDITGN
                     SVYAKFSGRPEVYLVPDYVVQNLVKTVDDLRDHSLIDTDLVQAGSLRVTADGRTIRVR
                     KAKDNSWDLTAPVSAKADPSKVRDVLTSLNDLRVDRFETDHPSDLKIYGLASPSAVVE
                     VGAAQAGAPDQTLLFGRQKLKTEDVFAKLAGSPTVFLVPQSFVKSLDLKPSDFRDKSL
                     LQFDASQAERLTVSHGGKTIVYVKDAQGRWQAVGRDQANDEGSGILSQLALLTVSDFP
                     GPKADSGTAHPAYRVDVALTGGLARHYAFGNREGSSVYLAVKGNPDLYLVPATAEAPL
                     GSLFGPATPAPTAAK"
     gene            1091..>1404
                     /locus_tag="VFR02_01415"
     CDS             1091..>1404
                     /locus_tag="VFR02_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9869142.1"
                     /translation="MPKRPPDQPLRFTEHLGALRGTLIRMAVACGLGVVLAFLKSDWL
                     FAALMRPFDRILEQTPVLAAKVQTLQTLTPVEAFMVNMKLATVAGLVLASPYLLREIW
                     VFA"
BASE COUNT          234 a          484 c          447 g          239 t
ORIGIN      
        1 gacgacagcg tctccgcccc cgcccacctg gcggattacg ggctggacca tcccggcgtc
       61 caggcgcagt tcaagttcaa ggatgggagc gcgcagaccc tgttggtggg ggacaaggac
      121 atcacgggga actcggttta cgccaaattc tccggccgtc ccgaggtcta cctggtcccc
      181 gactacgtcg tgcagaattt ggtcaagacg gtggacgacc tgcgggacca ttcgctcatc
      241 gacacggacc tggtccaagc cggcagcctg cgtgtgacgg ccgacggcag gacgatccgg
      301 gtgcgcaagg ccaaggacaa cagttgggac ctgaccgccc cggtctcggc caaggcggac
      361 ccctccaagg tgagggacgt gctcacgtcc ctcaacgacc tgcgggtgga ccgtttcgag
      421 accgaccacc cctcggacct gaagatctat ggactggctt ccccgagcgc ggtggtggag
      481 gtcggggccg cccaggccgg cgccccggac caaaccctgc tttttgggcg gcaaaaactg
      541 aagaccgagg acgtcttcgc caaactggcg ggatccccca cggtcttctt ggtgccccag
      601 tccttcgtca agagcctgga cctgaaaccc tcggatttcc gggacaagag cctgctgcag
      661 ttcgacgcca gccaggccga gcgcctgacc gtttcccacg gggggaagac catcgtctac
      721 gtgaaggacg cgcagggccg ctggcaggcg gtgggccgcg accaggccaa cgatgagggc
      781 agcgggatcc tgagccaact ggcgctcttg accgtctcgg atttccccgg cccgaaggcc
      841 gattcgggca cggcccatcc cgcctaccgg gtggatgtgg cgctcacggg cggcctggcc
      901 cggcattacg ctttcgggaa ccgggaaggc tcctccgttt atctcgcggt caagggcaat
      961 ccggaccttt acctggtccc ggccacggcg gaagccccgt tgggatcgct gttcggcccg
     1021 gccacgccgg cacccaccgc cgcgaaataa ccaactggcg cccgggcggc gatttgttta
     1081 tagttgaccc atgcccaagc gcccccccga tcagcccctc cgcttcaccg aacacctggg
     1141 cgcgctgcgc gggacgctga tccgcatggc ggtggcctgc ggcctgggcg tggtgctggc
     1201 cttcctcaag tccgactggc ttttcgccgc cctgatgcgt ccctttgacc gcatcctgga
     1261 acagaccccg gtcctggccg ccaaggtcca aaccctgcag accttgaccc ccgtggaagc
     1321 cttcatggtc aacatgaagc tggccacggt ggcgggcctg gtgctggcct ccccctattt
     1381 gttgcgcgag atctgggtct ttgc
//
LOCUS       DASTJJ010000114         1305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_17537, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000114 DASTJJ010000000
VERSION     DASTJJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17537"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR02_01420"
     CDS             complement(<1..206)
                     /locus_tag="VFR02_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869143.1"
                     /translation="MLEQYGTVLVFLVAGVFFILMAFLTAWLLGANGLLGYQQTHNLD
                     VFEDTYESGVNTIGTSWVRFNVSF"
     gene            complement(395..>1305)
                     /locus_tag="VFR02_01425"
     CDS             complement(395..>1305)
                     /locus_tag="VFR02_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9869144.1"
                     /translation="SALKTENPFIQQMVDYLARSQGKRVRPALAVLAARHCGRVTEET
                     IALGAALEMLHVATLIHDDIIDNSAVRRKQKTLNFKWGNEVSVLMGDYVFASSFYLMA
                     KNLPKDVLGILADTTNTICRGEISETANRFNVGLTLEEYEKIVREKTACLFAASCQTG
                     ALMAGAPPETTARFERFGLGIGMAFQIVDDTLDFVGRAKKVGKPVMSDLREGKLTLPV
                     LLCRERSSAAERKRIKAMILRKVKTPADFQRLAGLLKRTGAIELSLTRAKEWVEEAKA
                     AIAPLEEDRGKSELLRFADFLLDRDF"
BASE COUNT          264 a          402 c          389 g          250 t
ORIGIN      
        1 tagaagctga cgttgaaccg cacccaggag gtgccgatgg tgttgacgcc gctctcataa
       61 gtgtcttcga agacgtccag gttgtgggtc tgttggtacc ccaagagccc attggccccc
      121 agaagccagg ccgtgaggaa ggccatcagg ataaagaaga cccccgccac caggaacacg
      181 agaacagtgc cgtattgttc cagcataacg gtccttttgc gcgtggatga ctgatgggta
      241 aaccggttca tataaacacg accggcccac aaatatcaag gaaaacctat tccagagggc
      301 tgcgcgcgtc aacagggtcg atctttgtca cgaaaccacc gcgaggccga accgattaaa
      361 cctccggttg cacgaatcca ctccgcggga cgcgttagaa atcccggtcc aggagaaaat
      421 cggcgaaacg caggagctcg gacttaccac ggtcttcctc cagcggagcg atggcggcct
      481 tcgcctcttc cacccattcc ttggcccggg tcaaggaaag ttcgatggcc ccggtccgct
      541 tcaagaggcc ggccaggcgc tggaagtccg ccggcgtttt taccttgcgc aggatcatgg
      601 ccttgattcg cttgcgttcc gccgcggacg acctctcacg gcacaagagc acgggcagcg
      661 tcagcttgcc ttcgcgcagg tcgctcatga ccggcttgcc caccttcttc gcccggccca
      721 cgaaatccag cgtatcgtcc acgatctgga aggccatgcc gatgcccagg ccgaaccgtt
      781 cgaagcgggc ggtggtttcg ggcggggccc cggccatcag cgcacccgtt tggcagctgg
      841 ccgcgaaaag acaggccgtc ttttcccgca cgatcttctc gtattcttcc agggtcaacc
      901 ccacgttgaa acggttggcc gtttcggaga tctccccccg gcagatggta ttggtggtgt
      961 ccgccaggat gcccaacacg tccttgggca ggttcttggc catcaggtaa aaggaggaag
     1021 cgaagacata atcgcccatc aacaccgaga cctcgttgcc ccatttgaag ttcaaggtct
     1081 tctgcttgcg gcggaccgcc gagttgtcga tgatgtcgtc atggatcaag gtggccacgt
     1141 ggagcatctc cagggccgcg cccaaggcga tggtttcctc cgtgacccgc ccgcaatggc
     1201 gggccgccag gacggccagg gcgggccgga cgcgtttgcc ttgggaccgg gccaggtaat
     1261 cgaccatctg ttggatgaag ggattctcgg tcttcaaggc cgagg
//
LOCUS       DASTJJ010000115         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_385231, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000115 DASTJJ010000000
VERSION     DASTJJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385231"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFR02_01430"
     CDS             <1..226
                     /locus_tag="VFR02_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869145.1"
                     /translation="LPDETYMVAEVDADPEGHLAPWLVNFFQEGWPHTTFQNLRREAA
                     KKDIQPFPWIDALLASPGPATLASAPAAKP"
     gene            complement(223..720)
                     /locus_tag="VFR02_01435"
     CDS             complement(223..720)
                     /locus_tag="VFR02_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET9869146.1"
                     /translation="MGHLWIPDIPVWEKVLRSAVVYLFLLAAFRFAGKRQVGQLTPFD
                     LVVLLILSNVVQNAVIGNDDSLGGGLLGAVVILALNYGVVLLTFRYRPFRRLMAGEPS
                     LLVHNGRVFHDRLGREHLTMEDLTAALRKSGVADLAQVRFAILEDNGQISVIPREAPK
                     APPGS"
     gene            complement(808..1188)
                     /locus_tag="VFR02_01440"
     CDS             complement(808..1188)
                     /locus_tag="VFR02_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9869147.1"
                     /translation="MARENISTGSKWEPILGYSRAVKVGNYVHVSGTTGAGPDGKFDP
                     DPYVQTQRALKTIGEALAKAGASYQDVVRTRLYVTKIADWEKYGRAHGEVFGGIRPAM
                     AIVEVSKLIDPAMLVEIEADAYLG"
     gene            complement(1305..1625)
                     /locus_tag="VFR02_01445"
     CDS             complement(1305..1625)
                     /locus_tag="VFR02_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET9869148.1"
                     /translation="MADQDWQARLKTGRNDDCPCGSKKKYKKCHLQADEKAQSEALAK
                     ANAEAAEKAKAEGDGHEGHDHPGHEHHGHGHHGHSHGGGHTPKPGSPVVRQVSAPRKA
                     GGGS"
     gene            complement(1708..>2028)
                     /locus_tag="VFR02_01450"
     CDS             complement(1708..>2028)
                     /locus_tag="VFR02_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET9869149.1"
                     /translation="KVDQHPFEVYLGAQGIEHRTTRPASPFTNGFVERFHRTLKDEFF
                     GKVFREKFYTDLVELQKDLDEFLVFYNTQRAHSGYRCEGRTPWQTMEAQLTKASQEVG
                     PQAA"
BASE COUNT          339 a          702 c          593 g          394 t
ORIGIN      
        1 cctgccggat gagacctaca tggtggccga ggtggacgcc gaccccgagg ggcacctggc
       61 gccctggctg gtgaatttct tccaggaagg ctggccccac accaccttcc agaacctgcg
      121 gcgggaagcg gccaagaagg acatccaacc cttcccctgg atcgacgcgc tcctcgcctc
      181 cccgggcccc gccaccttgg cgtcggcccc cgcggccaaa ccctagcttc ccgggggcgc
      241 tttcggcgct tcccgcggga tcacgctgat ctgcccgttg tcctccagga tggcgaagcg
      301 cacctgggcg aggtccgcca cgccgctctt gcgcagcgcc gccgtcaggt cctccatcgt
      361 gaggtgttcc cggcccaggc ggtcgtggaa cacccggccg ttatggacca agagggacgg
      421 ttcccccgcc atcaggcgcc ggaaggggcg gtagcggaag gtgagcagca ccaccccgta
      481 gttcagggcc aggatcacca ccgcgcccaa gagccccccg cccagcgagt cgtcgttgcc
      541 gataacggcg ttctgcacca cgttggagag gatgagcagc accaccaggt cgaagggcgt
      601 caattgcccc acctggcgct tgcccgcgaa acggaacgcc gccagaagga agaggtacac
      661 caccgccgag cgcaggacct tctcccagac cgggatgtcc ggtatccata aatgtcccat
      721 ccgttcccct ttccaaaaag aaagcccctc cgtcattgtc ggaagggctt taactcctca
      781 cttctaactc cgaactccta actttcatta gcccagatag gcatccgcct cgatctcgac
      841 caacatggcc gggtcgatga gcttggacac ttccacgatg gccatggccg ggcggatccc
      901 gccgaacacc tcgccgtggg cccggccgta tttttcccag tcggcgatct tggtcacgta
      961 aagccgggtg cgcaccacgt cctgatagga ggcgcccgcc ttggccaggg cctcgccgat
     1021 ggtcttcagg gcccgttggg tctgtacgta ggggtccggg tcgaacttgc cgtccgggcc
     1081 cgcccccgtg gtgccggaaa cgtgcacgta gttcccgacc ttcacggccc gggaatagcc
     1141 caggatgggc tcccatttcg aaccagtact gatattttcc cgcgccattc cttcttctcc
     1201 ttaaggggtg gtatttgcat cggggcaaag ttgaaagcca gggtccgaag taaggcttaa
     1261 gaacttaaaa ctcctagttc ctaactccca actcctgacc gcttttaaga tccgccgccg
     1321 gccttccggg gcgcggagac ctgccggacc accggggacc cgggcttggg cgtgtggccg
     1381 ccgccgtgcg aatggccgtg gtggccgtgg ccgtggtgct catggccggg atgatcatgg
     1441 ccttcgtggc cgtcgccctc ggccttggcc ttctcggcgg cctcggcgtt ggccttggcc
     1501 agcgcctcgc tctgggcctt ctcgtcggcc tgcaggtggc acttcttgta ttttttcttc
     1561 gacccgcagg ggcaatcgtc gttgcgcccc gtcttcagcc tcgcctgcca gtcctggtcc
     1621 gccatttcaa gtcctcctgc caaagattaa tcacctgttc ttagcgcgag aagtttggac
     1681 aaatcctccg tctgaaccgg cccttcgtca agcggcctga gggcccacct cctgcgaggc
     1741 cttcgtcagc tgggcctcca tggtttgcca cggcgtccgc ccctcgcaac ggtaccccga
     1801 atgggccctc tgagtgttgt agaacaccag gaactcatcc aggtccttct gaagctccac
     1861 caggtcggtg tagaacttct cccggaacac cttcccgaag aactcgtcct tcaaggtccg
     1921 gtggaacctc tccacgaaac cgttcgtgaa aggactcgcc ggccgcgtgg tgcggtgctc
     1981 gatcccttgg gcgcctaggt acacctcgaa gggatgctgg tcgacctt
//
LOCUS       DASTJJ010000116         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_628692, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000116 DASTJJ010000000
VERSION     DASTJJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628692"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFR02_01455"
     CDS             complement(<1..443)
                     /locus_tag="VFR02_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869150.1"
                     /translation="MDKRDDHFLSQFFEKNDGLLRDRWRGALAHLTQRLARLDPDSRV
                     FAEMKKLIANLVALVQSTGSAEQAVDLELKSIVAQLRALQEDNRLTHAEMVLVLFFAR
                     DFLKDVLRDLMDASPEDQKPPFVEGLNQVSLLLNRLGLVFFENTMR"
     gene            complement(463..>1191)
                     /locus_tag="VFR02_01460"
     CDS             complement(463..>1191)
                     /locus_tag="VFR02_01460"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET9869151.1"
                     /translation="DRSRDLYGILNGIDYGEWNPATDKEIPAQYTPKNTDHKKEAKRL
                     LLRETGLKFHERTPVIGLVSRLADQKGLDLIAGILNEMLAGDVQFVVQGLGDARYHTL
                     FQELRDQFPDKFSLNLKFDNRLAKLIYAGSDLFLMPSRFEPCGLGQLIALKYGTVPVV
                     RRTGGLADTIEDLSPDGKRGTGFMFEEYRTEALWATLQRALEAFHQPKIWKGLVQRGM
                     TQDFSWDASAQRYLDLYRNIRFPA"
BASE COUNT          196 a          374 c          399 g          222 t
ORIGIN      
        1 cgcatggtgt tctcgaagaa caccaggccc agccggttga ggagcagcga cacctggttg
       61 aggccctcca cgaagggggg cttttggtcc tccgggctgg cgtccatcag gtcccgcagg
      121 acgtccttga ggaagtcacg ggcgaagaag aggaccagca ccatctcggc gtgggtgagc
      181 cggttgtcct cctgcagggc acgcaattgg gccacgatgg acttgagctc caggtccacg
      241 gcctgttcgg cgctgcccgt gctctggacc agcgccacca ggttcgcgat gagcttcttc
      301 atttcggcga agacccgcga atcggggtcc agccgggcca ggcgctgggt caggtgggcc
      361 agggccccgc gccaacggtc ccggagcagg ccgtcgttct tctcgaaaaa ttggctgagg
      421 aaatgatcgt cccgtttgtc catgaaagac cccaggcccg cgtcaggcgg ggaaccggat
      481 gttccggtaa aggtccaggt accgctgggc ggaggcgtcc caggaaaagt cctgggtcat
      541 gccgcgctgg accaggccct tccagatctt gggctggtgg aaggcttcca acgcccgctg
      601 gagggtggcc cacagggcct ccgtgcggta ttcctcgaac atgaacccgg tcccccgctt
      661 gccgtccggg gacaggtcct cgatggtatc ggccagcccc ccggtcctgc gcacgacggg
      721 cacggtcccg tatttcagcg cgatcaactg gcccaggccg caaggttcga accgggacgg
      781 catcaggaac aggtcgctgc cggcgtagat gagcttcgcc aaccggttgt cgaacttgag
      841 gttgagggag aacttgtccg ggaactggtc ccgcagttcc tggaagagcg tgtggtaccg
      901 ggcgtcgccc agcccctgga cgacgaactg cacgtccccg gcgagcatct cgttcaggat
      961 gcccgcgatc aggtccagcc ccttttggtc cgccaaccgc gacaccaggc cgatgaccgg
     1021 cgtgcgttcg tggaacttga ggcccgtctc ccgcaagagc agccgcttgg cttctttctt
     1081 atggtcggtg ttcttgggcg tgtactgggc ggggatctcc ttgtcggtgg ccgggttcca
     1141 ctcgccgtaa tcgatgccgt tcaggatgcc gtagaggtcc ctcgatcggt c
//
LOCUS       DASTJJ010000117         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1363378, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000117 DASTJJ010000000
VERSION     DASTJJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363378"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFR02_01465"
     CDS             complement(<1..633)
                     /locus_tag="VFR02_01465"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HET9869152.1"
                     /translation="MNNLAALRLCGEIGLAVLLFCGSPLFAQTAPPSWEFLPAEPLFA
                     PLIGDPREPHTAITAYGNENDFEGAVGDTMELVRYTPGDGTQWGWGLFGAGYILLSEN
                     GATFPMQAGDWLAGAYASEKSGPLAFRLEFEHRSSHLGDSLQGLVTPIFYSRENLNLA
                     FSWDAAAWLRLEGGLGFWENIAPPDKLFFASLGLEAYTPSTDFLGARLRGY"
     gene            complement(920..>1353)
                     /locus_tag="VFR02_01470"
     CDS             complement(920..>1353)
                     /locus_tag="VFR02_01470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9869153.1"
                     /translation="IAGEGPAKKRLEKLVEDMRMADIVHFAGYLSKEDWRDCYAGSDL
                     FVFASVTETQGLVVTEAMAAETPVVAVGEMGIKDVMASGKGGLMTRLDEAEFSAAVKR
                     MLTDGDFYRQKKSETLAEADKWSSTSMAKRMLEAYGKILKS"
BASE COUNT          262 a          432 c          405 g          254 t
ORIGIN      
        1 gtagcctcgc aggcgcgccc ccaggaagtc cgtggagggc gtataggcct ccagcccgag
       61 ggacgcgaag aaaagcttgt cgggcggcgc gatgttctcc caaaagccca gccccccctc
      121 caggcgcagc cacgcggccg cgtcccagga aaaggccagg ttcaggttct cccggctgta
      181 gaagatgggc gtgaccagcc cctgcagcga atcacccagg tgggagcttc ggtgctcgaa
      241 ttccaaccgg aaggccaggg gcccgctctt ttcactcgca taggcgcccg ccagccaatc
      301 ccccgcctgc atggggaagg tggccccatt ctcgctcaaa aggatgtagc ccgccccgaa
      361 gagcccccat ccccactggg tgccgtcgcc cggcgtgtag cggaccagct ccatcgtgtc
      421 gcccacggcg ccctcgaagt cgttctcgtt gccgtaagcc gtgatggccg tatggggttc
      481 ccggggatcg ccgatcaagg gcgcgaaaag cggctcagcg ggcaggaatt cccagctcgg
      541 aggggccgtc tgggcgaaaa gcggagaacc gcaaaaaagg agaaccgcca aacctatttc
      601 accgcagaga cgcaaagccg ccaagttatt caaagtggga acccctcagt ttttttggcc
      661 aattaagaca gttccaggct gactttgcgc ctttgcggtg aactggttcc agcttttaag
      721 gatcttttca taggcttcca gcatgcgctt ggcctacggg ggccgtctgg gcaaacaagg
      781 gggaaaaaac agaacaacgg gacccatttt accgcagagg cgcagagttg accaaaatga
      841 aaaaccctct gatgtttagg gttggatcca cctgttttcg cctaactttg cgtctccgcg
      901 tctttgcggt gaactggttc tagcttttga ggatcttccc gtaggcttcc agcatgcgct
      961 tggccatgga ggtggaggac catttgtcgg cctcggccag ggtctcggac ttcttttgcc
     1021 ggtagaagtc cccgtcggtc agcatgcgct tgaccgcggc ggaaaattcc gcctcgtcca
     1081 accgggtcat gaggccgccc ttgccggagg ccatcacatc cttgatgccc atctcgccca
     1141 cggccaccac gggggtctcg gcggccatgg cctcggtcac caccaggccc tgggtctcgg
     1201 tgacggaggc gaagacgaag aggtcggacc cggcgtagca gtcccgccag tcctctttgc
     1261 tcaaatagcc cgcgaagtgg acgatgtccg ccatgcgcat gtcctccacc agcttctcca
     1321 gacgcttctt ggccgggccc tcgcccgcga tga
//
LOCUS       DASTJJ010000118         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_385723, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000118 DASTJJ010000000
VERSION     DASTJJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385723"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1059
                     /locus_tag="VFR02_01475"
     CDS             <1..>1059
                     /locus_tag="VFR02_01475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9869154.1"
                     /translation="TPLPDTAFTVLCYHRFIIHPLKTTSPYLLPVAEFRAQMQYLKDN
                     GFTPITVDQLTAFWDQGTPLPPKPVLLSFDDGFKSVYDKAFPVLEEFHYPGVLFLISS
                     FLQTGDIWDARRKSDAGRLAMDDAEIKAMEKEGLVVESHTVSHPNLGVEAEKRAPDDF
                     KALVGRELGYPIDYFQSKFGGRPELLAYPYGVYNDEVLRQAKALYHFAFTVNEGPNDR
                     TVDPYKLRRDLILAGMPMAEFAGLFKDRVLHIDHAGPGDGQVIWSRKPLISIEILDDV
                     DPASVKLKLESRALKVHYDPALRLVTYRVQSPMRMGGHQLTLSAVGRDGQPRIYSWYF
                     RIKHRAQAAPPTPSAGPV"
BASE COUNT          190 a          383 c          319 g          167 t
ORIGIN      
        1 ccacccccct gcccgacacc gccttcaccg tgctctgcta ccaccggttc atcatccacc
       61 ccctcaagac cacgtccccc tacctgctgc ccgtggccga attccgcgcc cagatgcagt
      121 acctgaagga caacggcttc acgcccatca cggtggacca attgacggcc ttctgggacc
      181 agggaacgcc cctgccgccc aagcccgtgc tgctcagctt cgacgacggc ttcaagagcg
      241 tctatgacaa ggccttcccc gtgctggagg aattccacta tcccggggtg ctgttcctca
      301 tctccagctt cctgcagacc ggcgacattt gggacgcccg ccgcaagagc gacgcgggcc
      361 gcctggccat ggacgacgcc gagatcaagg cgatggaaaa ggaggggctg gtggtggaat
      421 cccacacggt cagccacccc aacctgggcg tggaggcgga aaagagggcc cccgacgact
      481 tcaaggccct ggtgggccgg gaactgggct atcccatcga ctacttccag tccaaattcg
      541 ggggacgccc cgagctgctg gcctatcctt acggggtcta caacgacgag gtcctgcggc
      601 aggccaaggc cctctaccac tttgccttca cggtgaacga ggggcccaac gaccgcacgg
      661 tcgaccccta caagctgcgc cgggacctga tcctggcggg gatgcccatg gcggaattcg
      721 ccgggctgtt caaggaccgg gtcctccaca tcgaccacgc ggggccgggc gacgggcagg
      781 tcatctggtc ccgcaagccc ttgatctcca tcgagatcct ggacgacgtg gaccccgcgt
      841 cggtgaagct caagctggaa agccgggcgc tcaaggtcca ttatgacccg gccctgcggc
      901 tggtcaccta ccgggtgcag tcgcccatgc gcatgggcgg ccaccaactg accctgtcgg
      961 cggtgggccg ggacgggcag ccccggatct acagttggta tttccgcatc aagcaccgcg
     1021 cgcaggcggc cccccccacc ccctcggccg gtccggtga
//
LOCUS       DASTJJ010000119         1863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1730885, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000119 DASTJJ010000000
VERSION     DASTJJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730885"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..556)
                     /locus_tag="VFR02_01480"
     CDS             complement(254..556)
                     /locus_tag="VFR02_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9869155.1"
                     /translation="MFLIANLLSALARILGMVCDLAILVILVRVILSWANADPYNGLV
                     RGVFALTEPLLSPFRRLVPPWKSGGLDLSTIFAVLGIELIQWFLVPSLYDMASRIH"
     gene            complement(579..>1863)
                     /locus_tag="VFR02_01485"
     CDS             complement(579..>1863)
                     /locus_tag="VFR02_01485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9869156.1"
                     /translation="GARARRAPAAGASKRPPGLKRFTLSDGSTLLVKRRPQVPLVSFG
                     AFFGGGFRDEKDAQAGVTSLMTKCLLKGTERLSYADLSRSVEGLAAHIDPVLEKDYWG
                     LTGECLSGRLDETFALFRETLLSPAFRREEVAKEKQMQLAALRRLKDDPADYALLKSD
                     VLTFAGTPYAHPPQGTSGSVSRLSRPEVLAWYGRFLASRNLTWVAVGDVEEERMRRLV
                     EDAARGIPRGKRAPSPRPSGRGAALRSYREKNKGQQANLVLGFRAPHFSHPDYFAFRV
                     MNTLLNGMGGRLFVELREKKSLAYSVYAAHEALAAAGIYQVYIGCAPEKQAQAREGLG
                     RVLADLAAGPIPAEELERAKTYLVGLYQVGQQANRSQLATLARYEMEGLGAEVVQDFP
                     QRVGRVSAGQVRRAAGKYLKMAEANWVVLEPRTRH"
BASE COUNT          333 a          639 c          606 g          285 t
ORIGIN      
        1 gccgagttgg gccatgggca cgctggcctg ggccaagccc gccgggttgg actccaccgc
       61 gtgggtgccc cccaccaagg cggtatccgc cccgcccatg cccagtccgt aggcgtcttg
      121 aaggggcagt ggatccaccg cccaggcggg ttgaacgttc cccccaagtg aaacaaggca
      181 agccagtgtc aggatgcgtg ggatgggcga tgatttcaaa gggacgattc tcctgccttc
      241 tgcacggctc ggcttaatgg atccgggacg ccatatcata aagggaaggg accaaaaacc
      301 attggattaa ttctatcccg aggaccgcga aaatggtcga caaatcaagc ccgccggact
      361 tccagggcgg caccagacgc cggaaagggc tcaagagggg ctcggtcagg gcgaagaccc
      421 cccggaccaa cccgttatag gggtccgcgt tggcccagga caggatgacc cgcaccagga
      481 tcaccaggat ggccaagtcg cagaccatgc ctaggatccg ggccagggcc gacaataggt
      541 tggcgatcaa gaacatggtc ctacctcctc gaacaagatc aatgccgggt ccggggctcc
      601 aagaccaccc aattggcctc cgccatcttc aggtacttcc ccgcggcccg gcgcacttgc
      661 ccggcgctca cccgccccac ccgctgcggg aagtcctgca cgacctcggc ccccaggcct
      721 tccatctcat agcgggccag ggtggccaat tgggaacggt tcgcctgctg cccgacctgg
      781 taaaggccga ccaggtaggt cttggccctt tccagctctt ccgccgggat gggtccggcg
      841 gccaagtcgg ccaagacccg gcccaacccc tcccgggcct gcgcctgttt ttccggggcg
      901 cacccgatgt agacctggta gatgccggcg gcggccagcg cctcatgcgc cgcgtagacg
      961 ctgtaggcca ggctcttttt ttcacgcaac tccacgaaca accgcccgcc catgccgttc
     1021 aagagggtgt tcatcacccg gaaggcgaaa taatccggat ggctgaaatg gggcgcgcgg
     1081 aagcccagca ccaggttggc ctgttgtcct ttgttctttt cccggtacga ccgcagggcc
     1141 gcgccgcgtc ccgacgggcg gggcgaaggc gcccgttttc cccgggggat gccccgggcg
     1201 gcgtcctcca ccaagcgccg catccgttcc tcctccacgt cgccgaccgc cacccaggtc
     1261 aggttgcggg acgccaggaa ccggccgtac caggccagga cctccggccg ggacaggcgc
     1321 gacaccgatc cggacgtgcc ctggggggga tgggcgtagg gcgtccccgc gaaggtgagc
     1381 acgtcggact tcaggagggc gtaatccgcc gggtcgtcct tcaaccggcg cagggcggcc
     1441 aactgcatct gcttttcctt ggccacttcc tcccggcgga aggccgggga aagcagggtc
     1501 tcgcggaaaa gcgcgaaggt ctcgtccagc cgcccactca ggcactcccc cgtcaggccc
     1561 caatagtcct tttccaggac cgggtcgatg tgcgcggcca ggccctccac cgaccgggag
     1621 aggtccgcgt aggacaggcg ttcggtgccc ttcaagaggc atttggtcat gagcgaggtg
     1681 accccggctt gggcgtcctt ttcgtcgcgg aaaccgccgc cgaagaaggc gccgaaggac
     1741 accaggggca cctgggggcg ccgcttcacc aaaagggtgg agccgtccga aagggtgaaa
     1801 cgcttcaggc ccgggggccg cttggaagcg cccgccgcgg gggcccggcg cgcgcgggcc
     1861 ccc
//
LOCUS       DASTJJ010000120         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_875087, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000120 DASTJJ010000000
VERSION     DASTJJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875087"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFR02_01490"
     CDS             complement(<1..772)
                     /locus_tag="VFR02_01490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9869157.1"
                     /translation="MAPSPTPAPRRVLVLRYSSLGDVALTNPVLDLIGRAWPGAEVYF
                     ATKPAYAPAVARHPALTAVVPLRAPGFLGLLDHLRRLRALGPDLVLDLHDSLRSRLAA
                     MALTRSRVLVYDKDAVNRRRLVHKMGGRPSLHTVQKYLKPLEEMGLPMPTRVPFTVAV
                     DRKGQRYLRDFLERRRIAPSQQVVGLGPGSLWATKRWPADRWAELAGRLARDHQCRLL
                     WFGSKEEAPLIRDIQGKMEGGERGINLAEDQNLEQSIAL"
     gene            complement(792..1913)
                     /gene="lpxK"
                     /locus_tag="VFR02_01495"
     CDS             complement(792..1913)
                     /gene="lpxK"
                     /locus_tag="VFR02_01495"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET9869158.1"
                     /translation="MGNLEQSYLKFLHEKPRSFSARLLGGLLGFLSLFYLAGWRAKRA
                     AHRAGFLRRRRLACPVISVGNLTTGGTGKTPVVIALARRFCAEGKRVAVLTRGYGRPA
                     SAPERLWVSDGKSLLATPETGGDEPVLIARKAPKAAVLVGKDRYRSGLEALEKFRPDL
                     FILDDGYQRRFEFHRDLDILVIDGTNPFSTGRVLPAGLLREPLEALADANIFVLNKVN
                     QARSPEDIRTVLQKHNPRAYVVESRYVPLRLRDLRTGQEVRPASLDRKPVGVFSGLGS
                     PLSVVRTLAEEKVFVRHAYHFPDHFAYTREKLREILDDAELRGLRYLVTTEKDEVKLP
                     PGLEPRVPILVLEIQWEVSGGKPQWETVLKTISLASSKT"
     gene            complement(1948..3234)
                     /locus_tag="VFR02_01500"
     CDS             complement(1948..3234)
                     /locus_tag="VFR02_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET9869159.1"
                     /translation="MAAPRKNAWTRLYGWLLDGLYLPLRSVSPVCPASFRERLGLYTP
                     EALRPLASGDNLWLHAASAGEVNAILPLARAFKARHPGAHIVLTTSSATGKRLALERQ
                     AADAVFLAPLDMTGPLRRAFRAFRPRLLLIAETEFWPAWLFRARRNQVPTLLVNGRIS
                     DKSFPSYQKMRGLFGPALQCFDQCLVQTEGDRDKLAALGVLEGKVRVLGQMKYDLAPP
                     DPAKAAEWGRVLGIREGDAWVVLGSLREGEEDLLFPQLPRLLAGHPGLRILVAPRHVK
                     NAALYQKKLGQMGLTSRLRSEAGEGSPSPVVVLDTVGELSLAYSLARAAFVGGTLVPV
                     GGHNVMEPALSGVPVLFGPHTGNVAEAAEALLSRGGGFRSGDPAQLAARMEGLLDGDV
                     SREAGQKARLAVESLRGATQKILEAVEAYWPGGPAV"
     gene            complement(3234..>3769)
                     /locus_tag="VFR02_01505"
     CDS             complement(3234..>3769)
                     /locus_tag="VFR02_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9869160.1"
                     /translation="ADTPEPPLWSLWHETILMSVWYHRRRDVHVMISASRDGELISTV
                     ARAFGYVPVRGSSSRGGPEAAAELVEYLKAGKRSAITPDGPRGPRRRLKPGVAKIAAL
                     SGRPVVPFAFEAEKCWRLKSWDRFIIPKPFSRAVFVYGEPLRFTGGDEGAFLDSIQAE
                     MDRVQDRAERHFGPGGS"
BASE COUNT          606 a         1292 c         1312 g          559 t
ORIGIN      
        1 ggagcgcgat ggactgttcc aggttctggt cctcggccag gttgatcccc cgctccccgc
       61 cctccatctt gccctggatg tcccggatca agggggcctc ctccttgctg ccgaaccaca
      121 agaggcggca ttggtggtcg cgcgccaacc ggccggccaa ttcggcccaa cggtccgccg
      181 gccagcgttt ggtggcccag agggacccgg gccccaggcc caccacctgc tgggaagggg
      241 cgatgcggcg gcgctccaaa aaatcccgca ggtagcgctg ccccttgcgg tcgacggcga
      301 ccgtgaaggg gacccgggtg ggcatgggca gccccatttc ctccagcggt ttgaggtatt
      361 tctgcacggt atgcagcgag gggcggccgc ccatcttgtg caccagtcgg cggcggttga
      421 cggcgtcctt gtcgtagacc aggacccggg aacgggtgag ggccatggcc gccaggcggc
      481 tcctgagcga atcgtgcagg tccagcacca ggtcgggtcc cagcgcccgc aggcgccgca
      541 ggtggtccaa aagcccgagg aaccccgggg cccgcagcgg caccacggcg gtgagggccg
      601 gatgccgggc cacggcgggg gcgtaggcgg gcttggtggc gaaatagacc tcggccccgg
      661 gccaggcccg gccgatcagg tccaggaccg gattggtgag ggccacgtcg cccagcgacg
      721 aatagcgcag gaccagcacc cgccgggggg cgggcgtcgg ggagggggcc aaggagtttc
      781 cttccgttca gtcaggtttt ggaggaggcc agcgagatgg tcttcaacac ggtctcccat
      841 tggggcttgc cgccgctgac ctcccattgg atctccagca ccaggatggg gacgcggggt
      901 tccagccccg ggggcagttt aacctcgtcc ttttccgtgg tcaccaggta tcgcaggccc
      961 cgcagctcgg cgtcgtccaa tatttcccgg agtttttccc gcgtataggc gaaatggtcg
     1021 gggaaatggt aggcgtgccg cacgaagacc ttttcctcgg ccagggtgcg caccaccgac
     1081 agggggctgc ccaagcccga gaagacgccc acgggcttgc ggtccaacga cgcgggccgc
     1141 acctcctgcc cggtcctcag gtcccgcagc cgcaggggca cgtaacgcga ttccaccacg
     1201 taggcccggg ggttgtgctt ttgcagcacg gtgcggatgt cctcggggct tcgggcctgg
     1261 ttcactttgt tgagcacgaa gatgttggcg tccgccaggg cctccagggg ttcccgcaaa
     1321 agccccgccg gcagcacccg gcccgtgctg aaggggttgg tcccgtcgat gaccaggatg
     1381 tccaggtcgc ggtggaactc gaagcggcgc tggtagccgt cgtccaggat gaagaggtcg
     1441 ggccggaact tttccagggc ttccaggccg ctgcggtagc ggtccttgcc caccagcacg
     1501 gccgccttgg gggccttgcg ggcgatgagc acgggttcgt cgccgcccgt ctcgggcgtg
     1561 gccagaaggg actttccgtc ggatacccag agccgctcgg gggcggacgc gggccggccg
     1621 tagccccggg tcagcaccgc cacgcgtttg ccctcggcgc agaatcggcg ggccagcgcg
     1681 atgaccaccg gcgtcttgcc cgtacccccg gtggtgaggt tgcccacgct gatgaccggg
     1741 caggccaacc ggcggcggcg caggaacccg gcgcggtggg cggcgcgctt ggcccgccag
     1801 cccgccaggt agaaaaggga caggaacccc agcagcccgc ccaggagccg ggcggaaaag
     1861 ctgcggggtt tttcatggag gaatttcagg taggactgct cgagattgcc catccgcccc
     1921 ttctgggttg aagcgtcggg ccgccgatca gacggccggc ccgcccggcc aataggcttc
     1981 cacggcctcc aatatcttct gggtggcccc ccgcagggac tccaccgcca agcgcgcctt
     2041 ttggccggct tcccgggaaa cgtccccgtc caaaagtcct tccatccggg ccgccagctg
     2101 cgccggatcc ccggaccgga aaccgccgcc ccgggacaac aacgcctccg cggcctcggc
     2161 cacgttgccc gtgtgggggc cgaacagcac gggcacgccc gacaaggccg gctccatcac
     2221 gttatgcccg cccacaggca ccagggtgcc gcccacgaag gccgcccggg ccagggaata
     2281 agccaaggat aactcgccca ccgtatccaa cacaaccacg ggcgaggggg aaccctcccc
     2341 cgcttcgctg cgcaagcggc tcgtcaatcc catttggccc agtttctttt ggtaaagcgc
     2401 cgcatttttg acgtgccggg gcgccaccaa tatcctcaag cccgggtgcc cggccaaaag
     2461 ccggggaagt tggggaaaga gcaggtcctc ctcgccttcc cgcaggctgc ccaagaccac
     2521 ccacgcatca ccctcccgga tgcccaggac cctgccccac tccgccgcct tggccgggtc
     2581 ggggggcgcg aggtcgtact tcatctggcc caggaccctt accttccctt ccaacacgcc
     2641 cagggcggcc agtttgtccc ggtccccttc ggtctgcacc aggcattggt cgaaacattg
     2701 cagcgcgggg ccgaagaggc cccgcatttt ttggtaggac gggaagctct tgtccgagat
     2761 ccggccgttg accaaaagcg tggggacctg gttgcgccgg gcccggaaga gccaggccgg
     2821 ccaaaattcg gtctcggcga tgagcagaag ccggggccgg aaagcccgga aggcgcgcct
     2881 taagggcccc gtcatgtcca gcggcgccaa aaagaccgcg tcggccgcct gccgttccaa
     2941 ggccagccgc ttccccgtcg ccgagctggt ggtgaggacg atgtgggccc ccgggtggcg
     3001 ggccttgaag gcccgggcca ggggcaggat ggcgttcact tcccccgcgg aggccgcgtg
     3061 cagccacagg ttgtcgcccg aagccagcgg ccggagcgct tcgggcgtgt aaaggcccag
     3121 acgttcccgg aaggaagcgg ggcaaaccgg ggagaccgat cgcaagggaa gataaagccc
     3181 gtccagcagc catccataga ggcgggtcca ggcgttcttg cggggcgcgg ccatcaggaa
     3241 cccccggggc cgaaatggcg ttcggcccgg tcttgcaccc ggtccatctc ggcctggatc
     3301 gagtcgagga aggccccctc gtcgccgccg gtgaagcgca gcggctcgcc gtagacgaag
     3361 accgcccggc tgaagggctt ggggatgatg aaacggtccc agctcttgag gcgccagcat
     3421 ttttcggcct cgaaggcgaa gggcaccacc gggcggcccg agagggcggc gatcttggcc
     3481 accccgggct tcaagcggcg gcgcgggccc cggggcccgt cgggcgtgat ggccgagcgt
     3541 ttgcccgcct tcaggtattc gaccagctcg gcggcggctt ccggcccgcc ccgggacgag
     3601 gatccccgca ccggcacgta gccgaaggcc cgcgccacgg tggagatgag ttccccgtcc
     3661 cggctggccg agatcatgac atgcacgtcc cggcgccggt ggtaccagac gctcatcagg
     3721 atggtctcgt gccagaggga ccagagcggc ggctcggggg tgtcggccc
//
LOCUS       DASTJJ010000121         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_629550, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000121 DASTJJ010000000
VERSION     DASTJJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629550"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="VFR02_01510"
     CDS             complement(<1..2157)
                     /locus_tag="VFR02_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9869161.1"
                     /translation="MDPRFTKNSGVETYTGNELIVKGGLEAGMALLTGYPGSPVADVF
                     EAAAAAAPYLKETGVLAQIANNEALSAARLNGAQMAGLRAVTVMKSVGFHVAADALVA
                     GSLAKKDHQGAGVIVIGDDPWSDTTQVPVDSRRLSDHLFIPVLEPSTFQEIKDWMKVA
                     FELSEEADLFIAYLLITYTAEGGGSVEVHPNQPPKGFSTKNKATLDSSTFDIKHSIVL
                     PPHTVPLEKEALEVKFPRVLEAARKKGINQVFKAAKKAPFAFITSGASYTYLSQALAE
                     FGLEGKFPILKLGMTYPVDPEAVKEILGHSTTLVVVEEKRRFIESQVRNIVMDLYQSG
                     QVKEMPTVWGKVFPDQLAGFPNDQGLSPAVVMRTLAPLFEKYEISEPRVRKEIAFLDS
                     LEQAEVSIPARTPSFCPGCPHRDSSSVFLQLVADFKDPAYMKKKHKRGPLDILFHGDA
                     GCYVLLFMPPNGPLMHNYIGMGLGGGTGAGLSPFSTNKSVCFIGDSTFFHSGLAEVSD
                     SIKNDQDVLYVVLDNKTTAMTGHQPHPGMEKDLMGNPTFGQDIEKVLQGFTAGEDVSI
                     ARVNPEARDDYRAILEDMLLEDGVKFIIADKECGITYHRRKRQELSRMKAGKGYLPVQ
                     KRINVNDHACEYCLECTRLTGCPGLAIEDTLYGPKMSTDLSYCVSDMACTRVKACPAF
                     EELTLVRGHKPAAPLLPDPASLPEPKPFEFGEDWRGY"
     gene            complement(2259..3323)
                     /locus_tag="VFR02_01515"
     CDS             complement(2259..3323)
                     /locus_tag="VFR02_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HET9869162.1"
                     /translation="MKWDISQRTLKIVLAAVFLLVSFIASFAAVLHNHAVPKPGMSER
                     ILITPGEEEQSQAGMAAVPTPTAAVAAVTAAGPESAETASEEKPPEEQISDSPQTFQL
                     GRLLAKGVTVTPYELKARDNYWTVADHHGINLYTLLGANPTLPFKAYLGQTLLLLSQR
                     GVLHEVYPNDTLKKIAIAYQVDVKAILDANTIHWWRGIRPGDVLFIPGAKPIRMADKW
                     RAYFDQRGFFGVPFSRGGEAITSGFGWRIDPINGKKEFHTGLDFRAKYGKSVFAAATG
                     RVTYAGVAGGYGNLIIVYHNKEYRTYYGHLSSILVKAGERVRRGQLIGKVGETGHATG
                     PHLHFEIRKYGKPIDPLPLI"
BASE COUNT          538 a         1017 c         1228 g          644 t
ORIGIN      
        1 gtaaccgcgc cagtcctccc cgaactcgaa gggcttgggt tcgggcagcg aggccgggtc
       61 gggcaggagg ggcgcggcgg gcttgtggcc ccgcaccagg gtgagttcct cgaaggccgg
      121 gcaggccttg acccgggtgc aggccatgtc ggacacgcag taggacaggt cggtggacat
      181 cttggggccg tagagggtgt cctcgatggc caggcccggg cagccggtca ggcgggtgca
      241 ttccaggcag tattcgcagg cgtggtcgtt gacgttgatg cgtttttgga ccggcaggta
      301 gcccttcccc gccttcatcc ggctcagttc ctgccgcttg cggcggtggt aggtgatgcc
      361 gcattccttg tcggcgatga tgaacttgac gccgtcctcc agcagcatat cctccaggat
      421 ggcccggtag tcgtcgcggg cctcgggatt gacccgggcg atggacacgt cctcgccggc
      481 cgtgaagccc tgcagcacct tctcgatgtc ctggccgaag gtggggtttc ccatcaggtc
      541 cttttccatg cccgggtgcg gctggtggcc cgtcatggcg gtggtcttgt tgtccagcac
      601 cacgtaaagc acgtcctggt cgttcttgat ggagtccgac acctcggcca gacccgagtg
      661 gaagaaggtg gaatcgccga tgaagcacac cgacttgttg gtggaaaagg gcgacaggcc
      721 ggcgccggtc ccgccgccca ggcccatgcc gatgtagttg tgcatgagcg gcccgttggg
      781 cggcatgaag agcagcacgt agcagcccgc gtcgccgtgg aaaaggatgt ccaggggccc
      841 gcgcttgtgt tttttcttca tgtaggccgg gtccttgaag tcggccacca gctgcaggaa
      901 caccgacgag gaatcgcggt gcgggcaacc cgggcagaag gagggcgtgc gggcggggat
      961 ggagacctcg gcctgttcca ggctgtccag gaaggcgatc tccttgcgga cccggggctc
     1021 ggagatctcg tatttctcga acaggggcgc cagggtgcgc atgaccaccg ccgggctcag
     1081 gccctggtcg ttggggaatc cggcgagctg gtcggggaag accttgcccc agacggtggg
     1141 catctccttc acctggcccg attggtagag gtccatcacg atgttgcgca cttgggactc
     1201 gatgaagcgg cgcttctcct ccaccaccac cagggtggtg gaatggccca ggatctcctt
     1261 cacggcctcc gggtccaccg ggtaggtcat gcccagcttc aggatgggga acttcccttc
     1321 caggccgaac tcggccaggg cctgcgacag gtaggtgtag gaggccccgg aggtgatgaa
     1381 ggcgaagggg gcttttttgg cggccttgaa gacctggttg atccccttct tgcgcgcggc
     1441 ctccagcacc cgggggaact tgacctccag ggcctccttc tccaggggca cggtgtgggg
     1501 cggcagcacg atggaatgct tgatgtcgaa ggtggagctg tccagcgtgg ccttgttctt
     1561 ggtcgaaaag cccttgggcg gctggttggg gtggacctcc acgctgcccc cgccctcggc
     1621 ggtgtaggtg atgagcaggt aggcgatgaa gaggtcggcc tcctcggaaa gctcgaaggc
     1681 caccttcatc caatccttga tctcttggaa ggtggagggc tccagcacgg ggatgaagag
     1741 gtggtcggag agcctccggg aatcgaccgg cacctgggtg gtgtcgctcc aggggtcgtc
     1801 gccgatgacg atgacgcccg cgccttggtg gtccttcttg gccaggctgc ccgccaccag
     1861 ggcgtcggcg gccacgtgga agcccacgga cttcatgacg gtgacggccc gcaggcccgc
     1921 catctgggcg ccgttcaggc gggcggcgga cagggcttcg ttgttggcga tctgggccag
     1981 gacgccggtt tccttcaggt agggggcggc ggcggccgcg gcctcgaaga cgtcggccac
     2041 gggggacccg ggatagccgg tgaggagggc catgccggcc tccaggcctc ccttgacgat
     2101 caactcgttg ccggtgtagg tctccacgcc ggaatttttg gtgaatcgag ggtccatgaa
     2161 ctgtttaact tacctttacg gggaggataa atcaataagg cagaaaggcc cgcaaaaaag
     2221 gccggattcg gtccggcccg cctttgtcac gtggttcgtc agatgagcgg cagggggtcg
     2281 atgggtttgc cgtatttgcg gatctcgaaa tgcaggtggg gcccggtggc gtggccggtc
     2341 tcgcccacct tgccgatgag ctggccgcgc cgcacgcgtt cccccgcctt gaccaggatg
     2401 ctggacaggt gcccgtagta ggtgcggtat tccttgttgt ggtagacgat gatcaggttg
     2461 ccgtagcccc ccgcgacccc cgcgtaggtc acccggcccg tggccgccgc gaagaccgac
     2521 ttgccgtact tggcccggaa gtccaacccc gtgtggaact ccttcttgcc gttgatgggg
     2581 tcgatgcgcc aaccgaagcc cgaggtgatg gcttccccgc cgcgggaaaa gggcaccccg
     2641 aagaagcccc gttggtcgaa ataggcgcgc catttgtccg ccatgcggat gggcttggcg
     2701 cccgggatga agagcacgtc gccgggccgg atgccccgcc accagtggat ggtgttggcg
     2761 tccaggatgg ccttcacgtc cacctggtag gcgatggcga tcttcttgag cgtgtcgttg
     2821 ggatagacct cgtgcagcac gccccgctgg gaaagcagca ggagggtctg gcccaggtag
     2881 gccttgaacg gcagggtggg gttggccccc agcagggtgt agaggttgat gccgtggtgg
     2941 tcggccacgg tccagtaatt gtcacgggcc ttgagctcat aaggcgtgac ggtcacgccc
     3001 ttggccagca accggcccaa ctggaaggtc tgggggctgt cgctgatctg ttcctcgggc
     3061 ggtttttcct cggaggcggt ctcggcggat tcgggccccg ccgccgtgac ggcggccacg
     3121 gcggccgtgg gggtggggac ggcggccatg ccggcctggg attgttcctc ctcgccgggg
     3181 gtgatgagga tgcgctcgct catgcccggt ttcggcacgg cgtggttgtg cagcacggcg
     3241 gcgaagctgg cgatgaagga gaccagcagg aacacggcgg ccaggacgat cttcaaggtg
     3301 cgctgagaaa tatcccactt catcctacga cacgaccccc agcgcgggtt tgaagcggcc
     3361 caagagcttc ttcacctcgg cggccacctg gtggggttcc aggccgcagt gctcgcgctg
     3421 caattgg
//
LOCUS       DASTJJ010000122         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_629630, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000122 DASTJJ010000000
VERSION     DASTJJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629630"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFR02_01520"
     CDS             complement(<1..712)
                     /locus_tag="VFR02_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9869163.1"
                     /translation="MTNPVQAPALVELKAVNKHYGPLHVLQDINMQVKKGEVVVVIGP
                     SGSGKSTLCRTINRLETIDDGSITIEGAELPKEGKGLAALRADVGMVFQSFNLFAHKT
                     ILENVTLGPIKVKGVSKADADKQALALLERVGVGHQSAKLPAQLSGGQQQRVAIARAL
                     AMKPKVMLFDEPTSALDPEMINEVLDVMVELAREGMTMVVVTHEMGFARKAADRVVFM
                     ADGQIVEEATPDEFFTNPQ"
     gene            844..>1259
                     /locus_tag="VFR02_01525"
     CDS             844..>1259
                     /locus_tag="VFR02_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9869164.1"
                     /translation="MSAPTGSSRSLRPLKKRIGSWTKGSPAGSTADARLLDTPPSSDF
                     THTDPWRVMRIQSEFVQGFDSLAGIGPAISVFGSARTKPGGRLYDLGVEVGRLLAEAG
                     VAVITGGGPGSMEAANRGAAEAGGLSIGLGIELPFE"
BASE COUNT          210 a          407 c          402 g          240 t
ORIGIN      
        1 tttgcgggtt ggtgaagaac tcgtccgggg tggcttcctc gacaatttgg ccgtcggcca
       61 tgaacacgac cctgtcggcg gccttgcggg caaagcccat ctcgtgggtc accaccacca
      121 tggtcatgcc ctccctggcc agttccacca tgacgtccag gacctcgttg atcatttccg
      181 ggtccagcgc gctggtgggc tcgtcaaaga gcatcacctt gggcttcatc gccagggcgc
      241 gcgcaatcgc cacgcgctgc tgctgcccgc cggaaagctg ggccggcagc ttggccgact
      301 ggtgccccac gccgacgcgc tccaacagcg caagggcctg tttgtcggcg tcggccttgg
      361 aaacgccctt gaccttgatg gggcccaggg tcacgttttc caggatggtc ttgtgcgcga
      421 acaggttgaa ggactggaag accatgccga cgtcggcccg caacgctgcc aggcccttgc
      481 cttccttggg cagttccgcg ccttcaatgg tgatggaccc gtcgtcgatg gtctcgaggc
      541 ggttgatggt ccggcagagc gtggactttc ccgagcccga gggcccaatg accaccacga
      601 cctcaccctt tttgacctgc atgttgatgt cctgcaaaac gtggaggggg ccatagtgct
      661 tgttgacagc cttgagttcc acgagggcag gtgcctgtac gggattagtc atgaagcgaa
      721 tctatccaac aatcccaagt gggtaaaact attccacggt tccgccctga tcgtaacgcg
      781 tttgatacct ggctcacacc gttcctaccc gcgggcaccg cggtttcacg tagccttagt
      841 ggcatgagtg cacccacagg atccagcaga tcccttcgtc ccttgaaaaa gcgcatcggc
      901 agctggacca aaggctcgcc ggccggctcg acggccgacg ccaggctcct ggacactccg
      961 ccaagcagcg acttcacgca caccgacccg tggcgggtca tgcggattca gagcgagttt
     1021 gtccagggct tcgattcgct ggccgggatc ggcccggcca tcagcgtgtt cggttccgcc
     1081 cgcaccaagc ccgggggacg gctctacgac cttggcgtgg aagtgggcag gctgctggcg
     1141 gaggccgggg tggccgtgat caccggtggc ggcccggggt ccatggaggc cgccaaccgg
     1201 ggcgccgccg aggccggggg cctgtccatc ggcctcggga tcgagctgcc gttcgagca
//
LOCUS       DASTJJ010000123         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1364397, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000123 DASTJJ010000000
VERSION     DASTJJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364397"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..995)
                     /locus_tag="VFR02_01530"
     CDS             complement(66..995)
                     /locus_tag="VFR02_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9869165.1"
                     /translation="MRLKPLLLLCLLLPWMAAWGHRQPPVTIGLCLDHGRGRELLLRL
                     LEKAMDENQARLITRDAQDDPAAQVRQAQDLIGRGAQALVVLPCDPARESALAEQARR
                     AGLKVVCLGQPLPSADYLIAFDRRKAGELQARALLAGHPRGRYLLLGKDREIRDGWME
                     ALGPAIRKGDVQVAASTRWDPRTGPVDAVLAPDAASAQEAVEALAKSGLRGQVPVSGV
                     GEDLPTCRRVLSGAQQSTVYRPAQKLAEETAYLSAKLARKAREFDCAFVEADNGASKI
                     PAVLLTPRLLGAKDLESAVAGDGLWEPSDLQGH"
BASE COUNT          188 a          453 c          452 g          205 t
ORIGIN      
        1 catcagcgtt catccgcatt catcattgca aaaggatctt tgctttgggg tgctgaaagc
       61 cggccctagt ggccctggag gtcggagggc tcccaaagcc cgtccccggc cacggcggac
      121 tccaggtcct tcgcccccaa aagccggggc gtcaagagca cggcggggat cttggaagcg
      181 ccgttgtccg cttccacgaa ggcgcaatcg aactcacggg ctttccgggc cagcttggcg
      241 gacaggtagg cggtctcctc ggcgagcttt tgggccggcc ggtagacggt cgactgttgg
      301 gccccggaca ggacccggcg gcaggtggga aggtcctcgc cgacgcccga caccggcact
      361 tgcccccgca ggcccgactt ggccagggct tccaccgcct cttgggccga agcggcgtcc
      421 ggcgccaaca ccgcgtccac cgggccggtc ctggggtccc agcgggtgga ggccgccacc
      481 tggacgtccc ctttccggat ggccgggccc agggcttcca tccagccgtc ccggatctcc
      541 cggtccttgc ccaacaagag atagcgtccg cggggatgcc ctgccaagag ggcccgggcc
      601 tgcagctcgc cggcctttcg gcggtcgaag gcgatgagat aatccgccga cgggaggggc
      661 tgccccaggc agaccacctt caagcccgcc cgccgggcct gctccgccag ggcggattcc
      721 cgtgccgggt cgcagggaag caccaccagg gcctgggccc cccggccgat caggtcctgg
      781 gcttggcgga cctgggcggc gggatcgtcc tgcgcgtccc gcgtgatcag ccgggcctgg
      841 ttctcgtcca tggccttctc cagtagccgc agcaggagtt cccggccccg gccgtggtcc
      901 agacagaggc cgatcgtgac cgggggctgc cggtggcccc aggcggccat ccagggaagc
      961 agcaggcaaa gcaaaagcag tggcttgagg cgcatccgtg tctcctgaga aggaatggcc
     1021 gaccccctca ttatagacgg cggccggccg ggggagctgg gaaaggggcc gggttgcgcg
     1081 atgggccctt cggggttaaa gttgaccctt ggccggtgcc gccccaatcc aacccgtgag
     1141 gtccctttgc gaggaatccc cgccgtcccc cggatcaccg cctgctggtt ctggttgctg
     1201 ctgggatcgg ccctcctggc ccagcccctg ggcgccttgg cccaggtgga cgatccgggc
     1261 ctgatccggg ccttggagac cgattcggct tcggtgga
//
LOCUS       DASTJJ010000124         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_752512, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000124 DASTJJ010000000
VERSION     DASTJJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752512"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="VFR02_01535"
     CDS             complement(<1..1645)
                     /locus_tag="VFR02_01535"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET9869166.1"
                     /translation="MKKRYLPTGNEYVSLPTIQEEDAALGDLNLAHLGLNGLLSFTGG
                     GQPLVTPRLFLDDAEALFPDRLTWDRDADWMPRFRFRDEQVELEGSFFCPVGERGFIL
                     ALKVTNLTDKAFYCVMGAETAWGGMQHSVNQTKPLKGERVLVKSWEDMPVLEWRAPQP
                     LAALALYPPDKASRFFYRLGKKALTPAGRETPLPEGETLTLQWLQSKDIKPRQFWETF
                     FYFGLGPEEIGAFASAREMQRVGGAALRDKTRAWLQARRLSTKDPELDPLLNRNAFFN
                     RFYATGRAVDTEEPVALTSRSPRYYVSGAYWDRDALLWSFPALLAQDRPWAGEVLAAV
                     FARQGRNFGTHSRYLSGAVLEPGFELDELCAAPIALRQYLDATRDFAILDKPGVLEGL
                     GAFERTLAAKRHPKIALYETWLLPSDDPWPQRYVTYDNVLVWRALLDLAEVRRHQEDK
                     KAFWRLRSQAEETRKAILRKCQVKGPKGRQFAWSVDLNKNFLLYDEPPGSLLLLPHWG
                     FCKPTFPAWKRTRDWIHSPDHRYSFQGKPFEEVGCAHAAH"
     gene            complement(1676..>2031)
                     /locus_tag="VFR02_01540"
     CDS             complement(1676..>2031)
                     /locus_tag="VFR02_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869167.1"
                     /translation="PRAAEHFKKARAAYGRALDEYTRAAEWYPAEPKYDYNRGNALVK
                     LGREEEALGAYAKAWTLDPRYAEAYFNGAIAAYHLGQYQRAGGYFQRVLDIDPRYPQA
                     REDLDTLVGRGWYHP"
BASE COUNT          330 a          661 c          691 g          349 t
ORIGIN      
        1 ggtgcgcggc gtgggcgcag cccacttcct cgaagggctt gccttggaag gaataacggt
       61 ggtcgggcga atggatccaa tccctcgtcc ttttccaggc cgggaaggtg ggtttgcaga
      121 aaccccaatg gggcagcagc agcaggcttc cgggcggctc atcgtagagc aggaagttct
      181 tgttcaggtc taccgaccaa gcgaactgcc ggcccttggg ccccttcacc tggcatttgc
      241 gcaggatggc cttgcgggtc tcctcggcct gggaccgcag gcgccaaaag gccttcttgt
      301 cctcctggtg ccggcggacc tccgccaggt ccagcagcgc ccgccagacc aggacgttgt
      361 cgtaggtcac gtagcgttgg ggccagggat cgtccgaggg caacagccag gtctcataaa
      421 gcgcgatctt gggatggcgc ttggcggcca gggtcctctc gaaggccccc aggccctcga
      481 gcacgcccgg tttgtcgagg atggcgaagt cccgggtggc gtccaggtat tgccgcaggg
      541 cgatgggcgc cgcgcagagt tcgtccaact cgaaccccgg ctccagcacc gcgcccgaaa
      601 gatagcggct gtgcgtgccg aagttgcggc cttggcgcgc gaagaccgcc gccagcacct
      661 ccccggccca gggccggtcc tgggccaaga gcgccgggaa ggaccagagc agggcgtccc
      721 ggtcccagta ggcgccgctg acgtaatagc gggggctccg ggaggtgagc gccaccggtt
      781 cttcggtgtc caccgcgcgg ccggtggcgt aaaagcggtt gaagaaggca ttgcggttga
      841 ggagggggtc caattccggg tccttggtcg agagccgcct cgcttggagc caggcgcggg
      901 tcttgtcgcg cagcgccgcg ccgcccaccc gctgcatttc ccgcgcggaa gcgaaggccc
      961 cgatctcctc gggccccagg ccgaaataga agaaggtctc ccagaactgg cggggcttga
     1021 tgtccttgga ttgcagccat tgcagggtca aggtctcgcc ctcgggcagc ggggtctccc
     1081 ggccggcggg cgtcagggcc ttcttgccca gccggtagaa gaagcggctg gccttgtcgg
     1141 gcggataaag ggccaacgcc gccagaggct ggggcgcgcg ccattccagg accggcatgt
     1201 cttcccagct cttgaccaac acccgctcgc ccttaagggg tttggtctgg ttcacgctgt
     1261 gctgcatgcc gccccaggcc gtctcggcgc ccatgacaca gtagaaggcc ttgtccgtga
     1321 ggttggtcac cttgagcgcc aggatgaagc cccgctcgcc cactgggcag aagaaactcc
     1381 cctccaattc cacttgttcg tcccggaaac ggaaacgggg catccagtcg gcgtcgcggt
     1441 cccaggtcag gcggtcgggg aaaagggcct cggcgtcgtc caggaaaagc cggggggtca
     1501 ccaggggctg gccccctccc gtgaaggaca aaagcccgtt caggcccagg tgggccaggt
     1561 tcaggtcccc cagggcggca tcttcctctt ggatggtcgg cagggaaaca tactcgttcc
     1621 ccgtcggcag atagcgtttt ttcatggcct tccccaacgt tgaggtagcc cgggcttagg
     1681 gatggtacca tccccggccc acgagggtgt ccaaatcttc ccgggcctgg ggataccgcg
     1741 ggtcgatgtc caacacccgt tggaagtagc cccccgcccg ctggtactgg cccaggtgat
     1801 aagccgcgat ggccccgttg aaataagcct cggcgtagcg gggatcgagc gtccaggcct
     1861 tcgcgtaggc gcccaacgcc tcttcctcgc ggcccagctt gaccaacgcg ttgccccgat
     1921 tgtagtcgta cttgggctcg gccgggtacc actcggccgc gcgggtatat tcgtccaggg
     1981 cccggccata ggccgcccgg gcctttttaa aatgctcggc ggcccggggg c
//
LOCUS       DASTJJ010000125         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_263802, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000125 DASTJJ010000000
VERSION     DASTJJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263802"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFR02_01545"
     CDS             complement(<1..508)
                     /locus_tag="VFR02_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9869168.1"
                     /translation="MLLRRYVIPAVLKMLRDLLKNAGTFLTKGKDDYIRVAVQVHDHK
                     ELMMGAAAILEGHWAEGRFSQDFSERLAEKMGTRFCLLTNSGSSSNLLALTALTSPLL
                     RGRRLKPGDEVVTVAAGFPTTVNPILQNGLTPVFVDVELGTYNTTLERVRDAIGPKTK
                     AVMLAHALG"
     gene            complement(605..985)
                     /locus_tag="VFR02_01550"
     CDS             complement(605..985)
                     /locus_tag="VFR02_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004507012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9869169.1"
                     /translation="MEKLLSMVGRVIYAVPFLVFGANHLLNAPVMAMMVPAYIPGGIF
                     WVYFTGLAMVLAGLAVITGIQGRKACFGLALMLAVFIVAVHLPGLADPQTKMDSLVNL
                     LKDTGLLGAALALAPTFPSKKTKQ"
     gene            complement(1051..1941)
                     /locus_tag="VFR02_01555"
     CDS             complement(1051..1941)
                     /locus_tag="VFR02_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9869170.1"
                     /translation="MNIFVTGGSGPLGHRVLKALVRNRHQVTAAATNPEETRWARQTG
                     ARPLKVNLFNLADTQVATIGQDVIINLTGGLGPGTWKKAHAARRAVSLSLVHAALKNR
                     PSRFIEESSFRIYRDHGLEEVGEEGALRSDPVAYSIQAMERNVLELGKAGPVPVVLRM
                     AEIYSADDSRTRRLLRWARLGYFAEMDPAPAYLPRVHADDAAAAVVHALRATAGVWNV
                     CEDQPLPRSKSARLLAEAMGRRELFKPPFWMARLAAPAEPSRESLNLSNRRFKTATGW
                     RPAYPSAKEGWGALLEQAAR"
BASE COUNT          388 a          714 c          729 g          357 t
ORIGIN      
        1 tgcccagggc atgggccaac atgacggcct tggtcttggg gccgatggcg tcccgcaccc
       61 tttccaaggt ggtgttgtag gtccccagtt cgacgtccac aaacaccggg gtcaacccgt
      121 tttggaggat gggattcacc gtggtgggga acccggcggc caccgtgacg acctcgtccc
      181 ccggcttcaa ccgccggccc cgcaacaggg gggacgtgag ggcggtgagg gccagcaggt
      241 tggacgaaga ccccgagttg gtcaggagac aaaaacgcgt ccccattttt tcggcgagtc
      301 tttccgagaa gtcctgggag aaccgcccct cggcccaatg gccttccagg atggccgccg
      361 cgcccatcat caattcctta tggtcgtgga cctggaccgc cacccggatg taatcgtcct
      421 tgcccttggt gaggaacgtc cccgcgttct tcaaaagatc ccgcagcatt ttcaagaccg
      481 cgggaatgac atatcgcctt aaaagcatgg gtgggttccg tgagggattt gggcaacggg
      541 gcattctaca gggccgggag gacgcaggac aaaggaaagg ggggaacccc cgtcctggac
      601 ggccctattg tttggttttt ttcgatggga acgtgggggc cagcgccagg gcggcgccca
      661 aaaggcccgt gtccttgagc aggttgacca gggaatccat cttggtctgg ggatcggcca
      721 agcccggcag gtggaccgcc acgatgaaga ccgcgagcat caaggccagg ccgaaacagg
      781 ccttgcggcc ctggatgccc gtgatgacgg ccagccccgc cagcaccatg gccaacccgg
      841 tgaaatagac ccagaagatg ccgcccggga tataggccgg caccatcatg gccatcaccg
      901 gcgcgttcaa gagatggttc gccccgaaga ccaggaaagg caccgcgtaa atgacccgcc
      961 ccaccatcga cagcagtttt tccatgaccg gctccttgtg cgtatggcgc tatggcccat
     1021 tctacccggc ccccaccggg ggaccaagcc tcagcgggcc gcttgttcca agagcgcgcc
     1081 ccacccttcc ttggccgaag gataggcggg ccgccacccc gtcgccgtct tgaagcgccg
     1141 gttggaaagg ttcagcgact cccgggaagg ttcggcgggg gcggccaagc gggccatcca
     1201 aaaaggcggc ttgaaaagtt cccggcggcc catggcttcc gccaacaacc gggcggactt
     1261 ggaccggggc aggggttggt cctcgcaaac gttccagacc cccgccgtcg cccgcagggc
     1321 atggaccacg gcggccgccg cgtcgtcggc gtggacccgg ggcaggtagg ccggagccgg
     1381 atccatctcg gcgaaatagc ccaggcgggc ccagcgcaac aggcggcggg tccttgaatc
     1441 gtcggccgaa tagatctccg ccatccgcag cacgacgggc accgggcccg ccttccccaa
     1501 ctccagcacg tttcgctcca tggcctggat ggaataggcc accggatccg accggagcgc
     1561 gccttcctcg ccgacctcct cgaggccatg gtcccggtag atgcggaagg aggattcctc
     1621 gatgaagcgg gacgggcggt tcttcagcgc cgcgtggacc aggctgagcg agaccgcccg
     1681 ccgggccgcg tgggcctttt tccaggtccc gggtcccagg ccgcccgtga ggttgatgat
     1741 cacgtcctgg ccgatggtgg ccacctgggt gtcggccaag ttgaacaggt tgaccttcaa
     1801 gggccgggcg ccggtctgac gcgcccagcg ggtctcttcg gggttcgtcg ccgccgccgt
     1861 cacctggtgg cggttgcgga ccagggcctt gaggacccgg tgccccagcg gccccgagcc
     1921 ccccgtcacg aagatgttca tgcccccttc tttccgcgcg gggcgggaag gcgaaaacgc
     1981 gtggatgaat tttgacccgc cgccctatcc ttattttaaa ggggcgctcg ggccgagggg
     2041 catgggaatc ggccggcttt tgggaaaacg acggtcatcc cgcctcaagc ccggtcgggg
     2101 accaggtcga tctgggtgat ctcggagggc gcccccaggc gcatgggcgg cccccagtag
     2161 cccgtgccgc agctgacgta aagctggg
//
LOCUS       DASTJJ010000126         1164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_508833, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000126 DASTJJ010000000
VERSION     DASTJJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508833"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..576
                     /locus_tag="VFR02_01560"
     CDS             34..576
                     /locus_tag="VFR02_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET9869171.1"
                     /translation="MGVELKGLTGSDLRSLETESAVPGLWTLEKAVGFIFKWARKNSL
                     WPLQFGTACCAIEMMATASGRYDLARFGAEVFRPSPRQADLLIAAGTITEKMGYALKK
                     LYDQMPKPSWVIAMGSCTIAGGPYYYDSYTVVKGIDSFIPVDVYVPGCPPRPESLIHG
                     LLAIQKKIEDEPIFRRRRLE"
     gene            632..1105
                     /locus_tag="VFR02_01565"
     CDS             632..1105
                     /locus_tag="VFR02_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9869172.1"
                     /translation="MTLEELKAKMEADFKEGILSAAVSTQKELIVILKPEAYHGVCAR
                     LRDGVEYAFDHLSCLTAVDHPKDAKITLVDHLWSYRRAQQVTLKVDLPRDNPRIPTVE
                     DIWKGANWLEREVYDLYGVVFEGHSDLRRIMMPEDYQKHPLRKDFSDDGFVVKPN"
BASE COUNT          240 a          357 c          352 g          215 t
ORIGIN      
        1 cctggcttac gcgtggaaaa aggaggcctt gaaatgggag tagagttgaa gggtctgacc
       61 ggttcggacc tccgcagcct ggagaccgaa agcgccgtgc cggggctttg gaccctggaa
      121 aaagccgtgg gcttcatctt caagtgggcc cgcaagaact ccctttggcc cctccaattc
      181 gggacggcct gctgcgccat cgagatgatg gccacggcct ccggccgcta tgacctggcc
      241 cgcttcggcg ccgaggtctt ccgtccctcg ccccgccagg ccgacctgct catcgccgcc
      301 ggaaccatca cggaaaaaat gggctacgcc ttgaagaagc tgtacgacca aatgcccaaa
      361 cccagttggg tcatcgccat gggcagctgc accatcgcgg ggggccccta ttattacgat
      421 tcctacacgg tggtgaaggg gatcgactcc ttcatcccgg tggatgtcta tgtgcccggg
      481 tgcccgccgc ggcccgagtc cttgatccat gggcttttgg ccatccagaa gaagatcgag
      541 gacgagccga tcttccgccg ccgccgcttg gagtgacgcc tttcgggcgc attcgacgcc
      601 ctacgagacc aaccagggag caagggaagc catgacgctg gaagagttga aggccaaaat
      661 ggaggcggat ttcaaggaag gcatcctttc cgccgccgtc agcacccaaa aggaactgat
      721 cgtgatcctc aagcccgagg catatcatgg ggtctgcgcg cggctccggg acggcgtcga
      781 atacgccttt gaccatctct cctgcctgac cgccgtggat catcccaagg acgccaagat
      841 caccctggtg gaccatctct ggtcctaccg tcgggcccaa caggtgaccc tcaaggtcga
      901 cctgccgcgg gacaatcccc ggatccccac ggtggaggat atctggaagg gcgccaactg
      961 gctggaacgg gaagtgtacg acctttatgg ggtcgtcttc gaggggcatt cggacctgcg
     1021 gcgcatcatg atgcccgagg actaccagaa gcatcctttg agaaaggatt tttccgatga
     1081 cggatttgtt gttaagccaa attaggaccg ggcaggagtc ccaattcggc acggacgagt
     1141 acgtcgtcaa cctggggccc caac
//
LOCUS       DASTJJ010000127         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1487927, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000127 DASTJJ010000000
VERSION     DASTJJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487927"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..851)
                     /locus_tag="VFR02_01570"
     CDS             complement(24..851)
                     /locus_tag="VFR02_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9869173.1"
                     /translation="MINVLKRHPLMLWGGLIVAALVVTALTAPILAPQDPKAQDLAQS
                     LLSPGWHHWLGTDQYGRDVLSRLLYGARVSLAVGFVAVSLYVLIGVTLGALAGYYGGW
                     VDLVLMRFVDVMLCIPTLFLILMVIAFVGPSILNVMVVIGLTSWMGVARLVRGEFLTL
                     KERDFVTAARVIGMTDARIILTQILPNALGPVLVVATLGVGSTILTESALSFLGLGVQ
                     PPTASWGNMLTEGKEHLTDAWWLVTFPGLAIFTTVLGYNLLGQGLRDWLDPRHQGKH"
     gene            complement(1071..2042)
                     /locus_tag="VFR02_01575"
     CDS             complement(1071..2042)
                     /locus_tag="VFR02_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9869174.1"
                     /translation="MKKFVLRRLLLMVPLLLGISILSFGIMQLAPGDPASLSAAMNQH
                     IDPSYIEKLRASYGLNDPLYLQYWHWLTRLCRLDFGDSFKDGRPVLAHILERLPATLL
                     LSGLSLALLFLLGVPLGVAAAYWQGRWPDKWITALTFFGFSMPDFWLALMMMLLFGVT
                     LGWFPVSGMWSLGAEDLPFFDRLGDLAAHLAMPLFVTTFVALASVSQYAKTSLLEVIR
                     QDYIRTARAKGLSESKVVLKHALRNALIPIVTLLGLSLPGLIGGSVIIESIFAWPGMG
                     RLGYESMMNHDYPLVMGVGILSAFLTLLGNLLADIGYAWLDPRVKYD"
     gene            complement(2110..2502)
                     /locus_tag="VFR02_01580"
     CDS             complement(2110..2502)
                     /locus_tag="VFR02_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9869175.1"
                     /translation="MSSQEMDESLDLIAKEIVDSAFKVHSTLGPGLLEQIYESCLVRE
                     LKKRNLPVERQQAVPVFYDGELMEPGLRLDLMVGGKVIVELKAVESLLPLHQAQLITY
                     LKLSGKKLGFLINFNVPVIREGIKRVVL"
     gene            complement(2557..>2912)
                     /locus_tag="VFR02_01585"
     CDS             complement(2557..>2912)
                     /locus_tag="VFR02_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HET9869176.1"
                     /translation="FDAVILGWTLSPEPDCYSIFHSSQTGEHQYNFVSYKDRTVDALL
                     VEGRRTLDPKKRKLIYQRLHQVLYEDQPYTFLYVPDAMPAIHKRFKGYTVNANGIATR
                     PEKWYVPAAQQKYQP"
BASE COUNT          634 a          884 c          912 g          482 t
ORIGIN      
        1 taatggtccg ggccggtccc ggctcagtgt ttgccctggt ggcgcgggtc cagccagtcg
       61 cgcaggccct ggcccagcag gttgtagccc aggaccgtgg tgaagatcgc gagccccggg
      121 aaggtgacca gccaccaggc gtcggtcagg tgttccttgc cctcggtgag catgttgccc
      181 caactggccg tgggcggctg cacgcccagc cccaggaaac tgagggccga ttcggtcagg
      241 atggtggatc ccacgcccag ggtcgccacc accagcacgg gccccagggc gttgggcagg
      301 atctgggtga ggatgatgcg cgcgtcggtc atgccgatga cccgggccgc cgtgacgaaa
      361 tcccgctcct tcagggtcag gaactcgccc cgcaccaggc gggccacgcc catccaggaa
      421 gtgagcccga tgaccaccat cacgttgagg atgctgggcc ccacgaaggc gatgaccatc
      481 aggatgagga agagcgtggg gatgcagagc atgacgtcca cgaagcgcat caggaccagg
      541 tccacccagc cgccgtaata gcccgccaag gcgcccaggg tcaccccgat gagcacgtag
      601 agcgacaccg ccacgaaacc caccgccagc gacacccggg ccccgtagag gagccgcgac
      661 agcacgtccc ggccgtactg gtcggtcccc agccagtggt gccagccggg ggaaagcagg
      721 ctctgggcca ggtcctgggc cttggggtcc tgcggcgcga ggatgggcgc cgtcagggcg
      781 gtgaccacca acgcggccac gatgagcccg ccccagagca tgagtggatg gcgcttcagg
      841 acgttgatca taaagccacc atcgacagct tattttcacc accaaggcac caagacacca
      901 aggtaatctc cgatctccca ctcgcgagaa ccacacccac accaactttg cataaaaatc
      961 caaagactaa accatccttg ggccttcctt ggtgtctgcc ccaacactga attgtggaaa
     1021 cggggccgcc cttgggcgtg ggtgccttgg tggtgaaaaa gattttctgt tcaatcatat
     1081 ttcaccctcg ggtccagcca ggcgtagccg atgtcggcca aaaggttccc caacagcgtc
     1141 aggaaggccg acaggatgcc cacccccatc accagcgggt agtcgtggtt catcatcgac
     1201 tcgtagccca gccgccccat gccgggccag gcgaagatgg actcgatgat gacgctgccc
     1261 ccgatcaaac ccggcaggga caggcccagc agggtcacga tggggatgag ggcgttgcgc
     1321 agcgcgtgct tgaggaccac cttggactcg gagagcccct tggcccgggc cgtgcggata
     1381 tagtcctgcc ggatgacctc caagaggctg gtcttggcgt actgggacac cgaggccagg
     1441 gccacgaagg tggtgacgaa cagcggcatg gccaggtggg cggccaggtc gcccaggcgg
     1501 tcgaaaaagg gcaggtcctc cgcgcccagg ctccacatgc ccgagaccgg gaaccagccc
     1561 agggtgacgc cgaagagcag catcatcatg agcgccagcc agaagtccgg catggagaac
     1621 ccgaaaaagg tgagggccgt gatccacttg tcgggccaac ggccctgcca ataggcggcc
     1681 gccacgccca ggggcacgcc gaggaggaaa agcagggcca gggacaggcc ggaaagcagc
     1741 agggtggcgg gcaggcgttc caggatgtgc gccagcacgg ggcgcccgtc cttgaaggaa
     1801 tcgccgaagt ccagccggca gagccgggtc agccagtgcc agtactggag atagagcggg
     1861 tcgttgaggc cgtaggaggc ccgcagcttc tcgatgtagc tggggtcgat gtgctggttc
     1921 atggcggccg acaacgacgc cgggtcgccg ggggccagct gcatgatgcc gaagctgagg
     1981 atggagatcc ccaaaaggag gggcaccatc aagagcagac gccgcaggac gaattttttc
     2041 acaaaaaacc ctcaaaccct atttcaccac caaggcacca agacaccaag gacggcaaaa
     2101 acgaatagtt cacaaaacaa cccttttgat accctcacga atgacaggaa cattgaaatt
     2161 gatcaaaaat ccaagcttct tgcccgacag tttgagatat gtgatgagct gggcttgatg
     2221 aagcggcaaa agggattcca ccgccttcaa ctcaacgatc acttttccgc caaccatcaa
     2281 gtccaagcgc agtcccggct ccatcaattc cccgtcataa aagacaggaa ccgcctgttg
     2341 cctctcaact ggaaggtttc gttttttcag ttccctcact aaacaagact cataaatttg
     2401 ttccaaaaga ccgggcccca acgtcgaatg gaccttaaag gccgaatcaa ctatttcctt
     2461 ggcgatcaga tcgagggatt catccatctc ttgagaactc atcagatcgg gcctttcggt
     2521 ccccttggtg ccttggtggt gaacaggcct tccgatttag ggctggtact tctgctgggc
     2581 ggcggggacg taccatttct ccgggcgggt ggcgatgccg ttggcgttca ccgtataacc
     2641 cttgaagcgc ttgtggatgg ccggcatggc gtccggcacg tagaggaagg tgtagggctg
     2701 gtcctcatag agcacctggt gcaggcgctg gtagatgagc ttgcgtttct tggggtccag
     2761 ggtgcggcga ccctccacca gcagggcgtc caccgtgcgg tccttgtagc tgacgaaatt
     2821 atactggtgc tcccccgtct ggctggaatg gaagatggaa tagcagtcgg gctcgggcga
     2881 gagggtccag cccaggatga ccgcgtcgaa ct
//
LOCUS       DASTJJ010000128         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1365321, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000128 DASTJJ010000000
VERSION     DASTJJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365321"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..993
                     /locus_tag="VFR02_01590"
     CDS             118..993
                     /locus_tag="VFR02_01590"
                     /inference="COORDINATES: protein motif:HMM:NF022720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HET9869177.1"
                     /translation="MKTSNLLPILFWAAAPAVWADDAALKALAAAPATVAAPVSAVDG
                     WTQSLLADLSLNQASFDNWKAGGTNYVSWQGDLHGKLERDNPGWNWLNTLHLAYGLTY
                     LDGQGTRKSADTMDFSSVLAWKAAPQWQPYVSLAFLSQFDTGFDYSTPSPTATSGFMD
                     PGYLTESLGVKYAPDPVFDTRLGAAVREAFADRFAAAYSDGKGVLMETGLNSVSDLDL
                     KLSTDSTLLSKLDLFWNGQGLDRTVADWQNGLTVHLGKIVALTAEYDLRYDPAVYLGW
                     QVKETLGIGVDYSLL"
     gene            complement(1247..>1908)
                     /locus_tag="VFR02_01595"
     CDS             complement(1247..>1908)
                     /locus_tag="VFR02_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869178.1"
                     /translation="IAAVARHAHGVRGVRGRAPLWVLAFGLLAAGCTPIGRGGFSGTS
                     YDSGTASGPVQASAQEAIRTAQDILAHQPDRQIDCSHFVLACYHSARMADYFRRQKPG
                     ENLTYYLNEYLTQARTRRAHAADIEPGDILIFNETYDLNHDGRIDGQDVYTHTGIVES
                     FQDWVVTYLDASLGRHPDIQRRRFSFYGDRYNETVARDPATGRAIHARETFYAAYAVP
                     P"
BASE COUNT          343 a          612 c          612 g          341 t
ORIGIN      
        1 cccaccctct cttaccaggg gagaaggaaa tggacccagc gggtttgctc ggtggatggt
       61 tccattataa taagcccttt caccgggccg tcggccaatt tctccaagaa gccgctgatg
      121 aaaacctcca accttcttcc catcttgttc tgggccgccg cccccgccgt ttgggcggac
      181 gacgcggcct tgaaggcctt ggcggccgcg ccggcgaccg tcgccgcgcc ggtcagcgcg
      241 gtcgacggtt ggacccagtc gttgctggcg gacctctcgc tcaaccaggc ctccttcgac
      301 aactggaagg ccggcggcac caattacgtt tcctggcagg gcgacctgca tgggaagttg
      361 gaacgggaca acccgggctg gaactggctg aacaccctgc acctggccta cggcctgacc
      421 tacctggacg gccagggcac ccgcaaatcg gcggacacca tggacttttc cagcgtgctg
      481 gcctggaagg ccgcccccca atggcagcct tatgtatccc tggctttctt aagccagttc
      541 gacacgggct tcgattattc caccccgtcc cccacggcca cctcgggctt catggacccg
      601 ggctacctga ccgagagcct gggcgtgaaa tacgccccgg acccggtctt cgacacccgc
      661 ctgggcgcgg ccgtgcggga ggccttcgcc gaccgtttcg ccgccgccta ttcggacggc
      721 aaaggcgtcc tgatggagac gggcctgaac tcggtctcgg accttgacct gaagctctcg
      781 accgattcca ccctgctgtc caagctggac cttttctgga acggccaggg cctggaccgc
      841 acggtcgccg actggcagaa cggcctgacg gtgcacctgg ggaagatcgt cgccctcacg
      901 gccgagtatg acctgcgcta cgaccccgcc gtgtacctgg gatggcaggt gaaggagacc
      961 ttggggatcg gcgtggacta ctcgctgctc taaagggcct ctaacaaaga tcaaaacgaa
     1021 gacgattttc accacacacg cccaagggcg gccccgctcc aaaaattcaa tgatggggca
     1081 gacaccaagg gcaccaagga cggccaagaa agaaaatccg agaaatccga atggatttta
     1141 ttgcctttct tggtgaactt cccccacatc ggtcttacgg gaacggggcc gcccttgggc
     1201 gcttgcgtct tggtgatgga acaggttttt ttggaagtcc tgaatttcag ggcgggacgg
     1261 cataggccgc gtaaaaggtc tcgcgggcgt ggatcgcccg gcccgtggcc gggtcgcggg
     1321 ccacggtctc gttatatcgg tccccgtaaa agctgaagcg ccgccgctgg atgtccgggt
     1381 gccgccccaa ggacgcgtcc aggtaggtca ccacccaatc ctggaagctt tccacgatgc
     1441 ccgtgtgggt gtagacgtcc tgcccgtcga tgcggccgtc gtggttgagg tcgtaggtct
     1501 cgttgaagat gaggatgtcg ccgggctcga tgtcggccgc atgggcgcgg cgcgtgcggg
     1561 cctgggtcag gtactcgttg aggtaatagg tgaggttctc gccgggcttc tgccggcgga
     1621 aatagtccgc catgcgggcc gaatggtagc aggccagcac gaagtgggag cagtcgatct
     1681 gccggtcggg ctggtgggcc aggatgtcct gggcggtgcg gatggcctcc tgggcggaag
     1741 cctgcaccgg gcccgaggcc gtgcccgaat cgtagctcgt gcccgaaaaa cccccgcggc
     1801 cgatgggcgt gcagcccgcc gccaagaggc cgaaggccaa gacccaaagg ggggcccgac
     1861 ccctcacgcc tcgaacaccg tgcgcatgcc gcgcgactgc agcgattc
//
LOCUS       DASTJJ010000129         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_509417, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000129 DASTJJ010000000
VERSION     DASTJJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509417"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..>1422
                     /locus_tag="VFR02_01600"
     CDS             59..>1422
                     /locus_tag="VFR02_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869179.1"
                     /translation="MLDPKATVFADLAAWTDKTTGQLVRLPKARQLEKADALKEAIRV
                     RISRALIGLTFGRPSIPPAPDQYGWYTARAVCLNRGWKSGMPLGRRARHNYLVLAAIF
                     YRVNHGMEEMAKAQAIVEAVDRGRYAAKHGALGDTLIYDESGAAARVAATQAAAAEEA
                     AARLAAEEEARQDDNPPASLEDLDPDGTNPGPVLLAQHEWRLRRTMERMKAHYAKQTP
                     KNPEQEKAFDRLHLWKRFVYSHLLLARAILDGERCDDAYALEEDLVKGRLSRLTLNYP
                     NTDMAPGEETMRSLHACLRLSDAVYAIPTGSSAGAATANAAAQVTTRLGADEAPMEET
                     MMTGSIPTTVRTAPDPSTNTNGEPPKKRRGRPPKNAVRPVGPTPRRPPSKPAAKNANK
                     GGPGRPPKGRGKKAKAAAAAAAAAKDLPMPLPLGTKLDGQDNEFQEEMNFYNRIAREE
                     DGAP"
BASE COUNT          308 a          478 c          447 g          189 t
ORIGIN      
        1 ctggctgccc gactctgagc cgagcacaga gtgggagcgc aacaaggcgt gcgacgtagt
       61 gcttgacccc aaggcaacgg tgtttgcaga ccttgcggcc tggaccgaca agaccacggg
      121 ccagctcgtg aggctgccaa aggcccgcca actcgaaaag gccgacgccc tcaaggaggc
      181 catcagggtg agaatctctc gcgccctgat tgggctcacg tttgggcgac cctccatccc
      241 tccggcgcca gaccagtacg gctggtacac ggcccgggcg gtatgcctca accgtggctg
      301 gaagagcgga atgcccctcg gtcgcagggc aaggcacaac tatctggtcc tcgctgcaat
      361 cttctaccgt gtgaaccacg ggatggagga gatggccaag gcccaggcaa ttgtcgaggc
      421 tgtcgaccgc ggccgttatg ctgccaagca cggcgccctc ggagacacgc tcatctatga
      481 cgagagcggg gccgctgccc gcgtggctgc cacccaagcg gcagctgcag aagaggccgc
      541 ggcgcgtctg gccgcagagg aggaggcccg acaggacgac aacccaccgg cctcccttga
      601 ggacttggac ccggacggaa ccaaccctgg gccggtgctg cttgcccagc acgagtggcg
      661 attgaggcgc accatggagc gcatgaaggc acactatgcc aagcagacgc caaagaaccc
      721 cgagcaagag aaagcctttg acaggctgca cctctggaag cgctttgtct actctcacct
      781 cctgctggca cgggccatcc ttgacggcga gcgctgtgac gatgcctacg ccctcgagga
      841 ggaccttgtc aagggcaggc tctcgaggct gaccctaaac tacccaaaca ccgacatggc
      901 ccccggcgaa gagaccatgc gcagccttca tgcctgcctg cgactctcgg atgctgtcta
      961 tgccattccc accggctctt cggccggtgc tgccacggcc aacgcggcgg cccaggtgac
     1021 caccaggctg ggggcagacg aagcacccat ggaagagacc atgatgacgg gcagcatacc
     1081 caccactgtc aggaccgcac ccgacccctc gacaaacacc aacggagagc cgcccaagaa
     1141 gaggcgcgga cgcccgccaa agaacgccgt caggcccgtg ggtcccacgc ctcgcaggcc
     1201 gcccagcaag ccggcagcca agaacgccaa caagggtggc ccgggcaggc cgcccaaggg
     1261 ccgtggcaag aaggccaagg ctgccgctgc tgcagcggcg gcagcaaagg acttgcccat
     1321 gccgctgccg ctcgggacca agctcgatgg ccaggacaat gagtttcagg aagagatgaa
     1381 cttttacaac cgaattgccc gggaagagga cggtgcccca ag
//
LOCUS       DASTJJ010000130         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_387896, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000130 DASTJJ010000000
VERSION     DASTJJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387896"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFR02_01605"
     CDS             complement(<1..1075)
                     /locus_tag="VFR02_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9869180.1"
                     /translation="MKPAPPLWGRPAKFLEHSLRPGRRIGWFVLFAALFAAQVVPRIF
                     QDSAYLDEAWETTSGYYYWLRGDAAPPYNHPPLANALQALPLVFLHLKVDPNVWQEGE
                     NRAYSLYYVSNPGRLGTILEAPRFLMLGFALAFGFLLYRVVLREKGAVFLAALFLWAF
                     DPTLLSFSIVSKADMPFSFFCFAGLLLFRRGQVERSWKWDALAGALAGAAVTSKMTAL
                     CLPVLYGGWDLWEAWKRPAFRGALVRRWAVGSAACAGVVFLVYFPGTLRLADHRWPFA
                     YFLSRVREALGTGGELGTVCGFLGGFYPRAEWWQVPVMFVLKSPLPFLALLALGGTLW
                     ARGKVRLPDWAWIFPIGWTALLCV"
BASE COUNT          283 a          377 c          369 g          127 t
ORIGIN      
        1 agacgcagag caaggcggtc catccgatcg ggaagatcca ggcccaatcg ggcaggcgca
       61 ccttcccgcg ggcccaaagc gtcccgccca gcgccagcag ggccaggaag ggcaggggcg
      121 atttcaggac gaacatgacc gggacctgcc accattcggc ccgcgggtaa aagcccccca
      181 agaacccgca aacggtgccc aactcgccgc cggtccccaa cgcctcacgc accctcgaca
      241 ggaagtacgc gaagggccat cgatggtccg ccaaccgcag ggtgccgggg aaatagacga
      301 ggaagaccac ccccgcgcag gccgctgatc ccaccgccca gcggcggaca agggcccccc
      361 ggaaagccgg gcgtttccag gcttcccaca ggtcccatcc cccgtaaagg acgggcaggc
      421 agagggccgt catcttggac gtgaccgccg ccccggccag ggccccggcc agggcatccc
      481 atttccagga ccgttccacc tgtccgcgtc ggaagagcaa caaacccgcg aaacaaaaaa
      541 aagaaaaagg catgtccgcc ttggagacga tggagaagga cagcagagtc ggatcgaagg
      601 cccagaggaa cagggccgcc aggaagacgg cgcctttctc ccgaaggacc acccggtaga
      661 gcaaaaatcc gaaggccagg gcaaaaccca gcatgaggaa ccggggcgcc tccaggatcg
      721 tccccaaccg gcccggattg gagacgtagt agagggaata ggcccggttc tcgccttcct
      781 gccaaacgtt cggatccacc ttcaaatgca ggaacaccaa aggaagggcc tggagggcgt
      841 tggccagggg agggtgatta tagggagggg ccgcgtcgcc ccggagccag tagtaatatc
      901 ccgaggtcgt ttcccaagcc tcgtccagat aggccgaatc ctggaagatg cgggggacca
      961 cctgggccgc aaagagggcg gcgaaaagga cgaaccaccc aatgcgccgg ccgggccgca
     1021 gggaatgttc caggaatttc gccggcctgc cccaaagcgg aggagcaggt ttcacccgca
     1081 aacgacggtc gatggcccac cccttcgaga aaaaaatgat ggaccggttc cagcggccgc
     1141 ggaacgaaaa aggcgg
//
LOCUS       DASTJJ010000131         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_143252, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000131 DASTJJ010000000
VERSION     DASTJJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143252"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1030)
                     /locus_tag="VFR02_01610"
     CDS             complement(125..1030)
                     /locus_tag="VFR02_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9869181.1"
                     /translation="MSASTTARRARPAKPGAGRSRPHYGTHLFLTVMAVMWMVPLGWA
                     LFTALRPKADTDKYGYFSLGGAFNFNNFAQAWTQGGFPRYFMNSLIITVPTIILVLLF
                     SSMMAFAVSRVSWKFNVTLLIMFTAGNLLPPQVLATPLFEMFKLIQLPYWFSDSGSLL
                     NTYISVIIANTAFQIGFCTFVLSNYMKALSADLTEAALVDGAGIWRQYVSIIMPLCRP
                     AIAALATLELIFIYNDYFWPLLFIQSGDRLPVTTAINNLQGTFLSNYNLLAAGATITV
                     VPTLIIYIVLQRHFVAGLTLGSSKG"
BASE COUNT          197 a          267 c          388 g          182 t
ORIGIN      
        1 gacgattgcg gtgcgccggg cgtcggcgag cattggcacg tcttcggtgc ggtcggaccg
       61 ctccgtgtgg ggcagggggg cgcccttggt ggctttcatg tgcttcccgg ttctcacgcc
      121 accctcagcc cttgctggag ccgagggtca ggccggcaac aaagtggcgt tgcaggacga
      181 tgtagataat cagggtcggg accaccgtga tggtggcccc ggcggccagc aggttgtagt
      241 tggagaggaa cgtcccctgc aggttgttga tggccgtggt gacggggagc ctgtcgccgc
      301 tttggatgaa gagcaggggc cagaagtagt cgttgtagat gaagatcagt tccagcgtgg
      361 ccagcgccgc gatggccggg cggcacagcg gcatgatgat tgatacgtac tggcgccaga
      421 tgcccgcgcc atccacgagg gcggcctcgg tcaggtccgc cgagagcgcc ttcatgtaat
      481 tggagagcac gaaggtgcaa aagccgatct ggaacgcggt gttggcaatg atgacggaaa
      541 tgtaggtgtt gagcaggctg cccgaatcgc tgaaccagta cggcagctgg atcagcttga
      601 acatttcaaa gagcggggtg gccaggacct ggggcgggag caggttgccg gccgtgaaca
      661 tgatcagcag ggtgacgttg aacttccagc tcacccggct gacggcaaag gccatcatgg
      721 aactgaacag cagcaccagg atgatggtgg ggacggtgat gatgagcgag ttcatgaagt
      781 agcgcgggaa gccgccctgc gtccaggcct gtgcgaagtt gttgaagttg aatgcgccgc
      841 cgaggctgaa atagccgtat ttgtccgtgt ccgccttggg gcgcagggcc gtgaacaggg
      901 cccagcccag ggggaccatc cacatgactg ccatgacggt gaggaaaagg tgggtgccgt
      961 agtgcgggcg gctgcggccc gcccctggct tggcgggccg ggcccggcgg gcggtggtgg
     1021 aagcagacat caga
//
LOCUS       DASTJJ010000132         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1488850, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000132 DASTJJ010000000
VERSION     DASTJJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488850"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..538)
                     /locus_tag="VFR02_01615"
     CDS             complement(158..538)
                     /locus_tag="VFR02_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9869182.1"
                     /translation="MAPNPLDQRVAPRLPVNFHIRFRLLSSPSGEPPEPSLHNGNNLM
                     ANLSRTGFFLSTKNYLEVHSHIEVEVPLADGEAPFLGEAEVVRSNYHNFPSQGRYEYG
                     LKFTLLHSRAQENLLNFLQKAGWA"
     gene            complement(569..>1029)
                     /locus_tag="VFR02_01620"
     CDS             complement(569..>1029)
                     /locus_tag="VFR02_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HET9869183.1"
                     /translation="SPVISNNEIYNNRYGFYLKDFGTPTISGNHIYNNTNYNIVNYSP
                     KSLALADNDFRQTDPNMIARTIYDGLYNPRLGRVTFPSSAGMNATQMASNPSARPTPN
                     VQEEDLWSYGRPFDAMKISNIDAQKKKPSGTLKVLAVGATAVVTAVLLFL"
BASE COUNT          199 a          271 c          347 g          212 t
ORIGIN      
        1 ccttgaaaaa tttaagtccg gaaaacaggc gtccgacatt ggacgaaaaa cggggcggac
       61 aggttcccct tccggcgggc ccggcggatc atccgcctgg accggcccgg gacgaggcag
      121 gattctatca cggccccccg accgggacaa tccgggctca ggcccagccg gccttttgga
      181 ggaaattcag gaggttttcc tgggcgcggg agtgcaaaag ggtgaatttc aacccgtatt
      241 cgtaacgccc ctgggaagga aaattgtggt agttcgaccg gaccacctcg gcttccccga
      301 ggaaaggcgc ctccccgtcg gccaggggga cctccacctc gatgtgggaa tggacttcca
      361 ggtagttctt ggtggaaagg aaaaaacccg tccggctcaa attggccatc aggttgttgc
      421 cgttatgcag gctgggttcg ggaggttccc cgctggggga gctcaaaagg cggaatcgga
      481 tgtggaaatt gaccggcagg cgcggggcca cgcgctgatc cagcggattg ggtgccatcg
      541 ttccgctccg gcggcccggg gccgtctttt ataggaaaag caggaccgcc gtgaccaccg
      601 ccgtcgcgcc cacggccagg accttcaggg tgccggaagg tttctttttc tgcgcatcga
      661 tgttgctgat cttcatggcg tcgaaggggc gcccatagct ccagaggtcc tcttcctgca
      721 cgttcggggt cgggcgggcg gaaggattgg aggccatctg ggtggcgttc atcccggccg
      781 aactggggaa ggtcacccgg cccaggcggg ggttgtagag gccgtcatag atggtgcgcg
      841 cgatcatatt cgggtcggtc tgcctaaaat cgttatcggc caaagccaaa gatttcggcg
      901 aatagttcac gatgttgtag ttggtgttgt tgtagatgtg gttccccgag atggtggggg
      961 tgccgaagtc cttcaaataa aagccgtaac ggttgttgta gatctcgttg ttggaaatga
     1021 ccgggctgg
//
LOCUS       DASTJJ010000133         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_510345, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000133 DASTJJ010000000
VERSION     DASTJJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510345"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VFR02_01625"
     CDS             <1..1225
                     /locus_tag="VFR02_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9869184.1"
                     /translation="LMNAGYLAYWEKTRISTHSHRELTRRAGNLLHVQMGGDFLLLTV
                     MLYLGGGAGNPLVLFYLFHVAIAVAVFPPGESVFYVPLAVALPWLLSLLPPFASPAPS
                     FVVGGGSLEPVRAFLWAYSAAAVGLWFFLSRLAQDIRAREGELKVQTLDLRTAKEGLE
                     QLDLVKNRFFAQVAGRLEAPAQEIEDHFQRMEKQVPDSWPEGREMVREARRHGQALRS
                     TVEDLLWISRLEAKDFALRKESIAAYAEALRCVQRLEPQARSKGVEFKLHGSDQVRLW
                     ADREGFDRVLDNLLSNAVKYGPAKGGTVTLDLEVKPDWLVVSVQDEGIGIAADQKKRV
                     FGDFFRAPNAQAREKFGTGLGLAIVRRVLEWHGGKVEFSSHLKHGTRVETWWPLLPSV
                     KGGMDLEVGVEGGRS"
     gene            1282..1902
                     /locus_tag="VFR02_01630"
     CDS             1282..1902
                     /locus_tag="VFR02_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869185.1"
                     /translation="MENSAWVPARLGLALLLAGAAFPARAADLAPNQQWIEQVNAGAV
                     LPLSTKVSGAFAPGYGGEIVVGYRFDRDFVLSTSFGYYDCDQKGSGATAGEWLYMPLM
                     ESARLNMGEGWFRPYLLLGLGAAFNTYSETVPFGASTQKLSHGETDLLVSTGVGGLFI
                     LFPNSALYVQGRLDLDFTTGKSLSSPAVDDPTLFIPLQAGISFFVL"
     gene            2106..3236
                     /locus_tag="VFR02_01635"
     CDS             2106..3236
                     /locus_tag="VFR02_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9869186.1"
                     /translation="MREAENQLSRFRGILEAAPDAMVIVDRTGAIILVNAQAETLFGY
                     PRQELLGKPIESLIPERFRRRHPGHRDGFFASPQLRPMGAGLDLYALRKDGTEFPAEI
                     SLSPVETPEGTFVTAAVRDVSDRKRQMEEENRRVAEANRLKSEFLANMSHELRTPLNA
                     IIGFSELMHNGKVGPVSDEHKEYLGDILTSARHLLQLINDVLDLTKVEAGKMEFRPEL
                     VDLPKVVSEVKEILRGLAAPKRLKLEVAVDPALDRVVLDASKLKQVLYNYLSNAIKFT
                     PEDGRVTLRAFPEGADRFRLEVEDTGIGIQEGDLHKLFIEFQQLDAGSAKKYAGTGLG
                     LALTKRIVEAQGGEVGVKSRPGEGSVFWAVFPREWKGEPNAR"
     gene            3226..3597
                     /locus_tag="VFR02_01640"
     CDS             3226..3597
                     /locus_tag="VFR02_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9869187.1"
                     /translation="MPGEEILIVDDNAVNLKLARVVLMGAGYAVKTAGSAEEALKLLE
                     TLHPALILMDLQLPGMDGLELTRRLKAAPETRSVKVVALTAYAMKGDEEKARAAGCDG
                     YLAKPIDTQKFSAQVAGYLER"
BASE COUNT          617 a         1111 c         1250 g          630 t
ORIGIN      
        1 gctcatgaac gccggctacc tggcctattg ggaaaagacc cggatctcga cccattccca
       61 ccgggaactg acccgccggg cggggaacct gttgcatgtc cagatgggcg gggatttcct
      121 gttgctcacc gtcatgttgt acctgggcgg cggggcgggc aatcccctgg tgctcttcta
      181 cttgttccat gtggccatcg cggtggcggt cttcccgccg ggggaaagcg tcttttacgt
      241 gcccttggcc gtcgccctgc cctggttgct gagcctgttg ccccccttcg cgtcgcccgc
      301 cccatccttt gtggtcgggg gcggttccct ggagccggta cgggcctttc tctgggccta
      361 ttcggccgcc gcggtgggat tgtggttctt cctgtcccgg ctcgcgcagg acatccgggc
      421 gcgggaaggc gaactgaagg tccagaccct ggacctgcgg acggccaagg agggcctgga
      481 acagttggac ctcgtcaaga accggttctt cgcccaagtg gcggggcgct tggaagcccc
      541 cgcccaggag atcgaggacc actttcaacg catggaaaaa caggtgccgg attcctggcc
      601 cgaggggcgg gaaatggtgc gggaagcccg gcggcatggg caggccctcc ggtccaccgt
      661 ggaggacctg ctttggatct cccggttgga ggccaaggat tttgccctgc gcaaagagtc
      721 catcgcggcc tacgcggagg cgttgcgctg cgtccagcgc ctggaaccgc aggcccggag
      781 caagggcgtg gagttcaagc tgcacggcag cgaccaagtg aggctctggg cggaccggga
      841 agggttcgac cgggtgttgg acaacctgct ctcgaacgcg gtgaagtacg gccccgcgaa
      901 gggggggacg gtgaccctgg acctggaggt gaagcccgat tggctggtgg tgtcggtcca
      961 ggacgagggg atcggcatcg ccgcggacca gaaaaagagg gtcttcggtg attttttccg
     1021 cgcgcccaac gcccaggccc gggaaaaatt cggcaccggc ctggggctgg ccatcgtgcg
     1081 gcgcgtcctg gagtggcacg gcggcaaggt ggagttcagc agccatctca agcacggtac
     1141 ccgggtggag acctggtggc ccctgctgcc gtcggtgaag ggcgggatgg accttgaagt
     1201 aggtgtggaa ggaggacggt catgaaaaga acgggatggt tttggggatg gcgggcgttg
     1261 gcttgggcct gccttggatg tttggagaat tccgcgtggg ttccggcgcg gttggggttg
     1321 gccctgctgt tggcgggggc ggccttcccg gcccgggcgg cggacctggc gcccaaccaa
     1381 cagtggatcg agcaggtgaa cgcgggggcg gtgttgccgt tgagcaccaa ggtctcaggg
     1441 gccttcgcgc cgggttacgg cggcgagatc gtggtgggtt accgctttga ccgggatttt
     1501 gtcctttcca cttccttcgg ttattacgat tgcgaccaga aggggtcggg ggcgaccgcg
     1561 ggggagtggc tttacatgcc gctgatggag agcgcccgct tgaacatggg ggagggttgg
     1621 ttccggccct acctgctcct gggcctgggc gcggccttca acacctacag cgagaccgtg
     1681 cccttcgggg catcgaccca aaagctttcc cacggggaaa cggacctgct ggtctcgacg
     1741 ggggtggggg ggttgttcat ccttttcccc aactcggccc tctatgtgca ggggcggttg
     1801 gacctggact tcaccacggg aaagagcctg tccagcccgg cggtggacga tcccacgctt
     1861 ttcatccccc tgcaggccgg gatcagcttt ttcgtccttt gaagcgggtt tcacggggtt
     1921 tgtggcccgg taaaacctgg ccggggccac cctattcgct tggaaagggc aggatgaaaa
     1981 tgtagaattt ttcatccact tttccaggcg gtcttgtgaa gccgaagaaa aaaaccaggg
     2041 aacccaaaag accggcgaac gcccaagcgc ccccagccgc cccgtcaagc gcggagacgc
     2101 ggcgcttgcg ggaagccgag aaccagttgt cccgtttccg gggcatcttg gaggcggccc
     2161 ccgacgccat ggtcatcgtg gaccgcacgg gcgcgatcat cctggtcaac gcccaggccg
     2221 agaccctctt cggctacccc cgccaggaac tgctgggcaa gcccatcgag tccctcatcc
     2281 ccgagcgctt ccgccgccgc cacccgggcc accgggacgg ttttttcgcc tcgccccagt
     2341 tgcgccccat gggggcgggg ttggacctct acgccctgcg caaggacggc accgaattcc
     2401 ccgccgagat cagcctgagc ccggtggaga cgcccgaggg aaccttcgtg acggccgcgg
     2461 tgcgcgacgt cagcgaccgc aagcggcaga tggaggagga gaaccgccgc gtcgccgagg
     2521 ccaaccggct caagagcgag ttcctggcca acatgtccca cgaactgcgc acgccgctca
     2581 acgccatcat cggcttctcc gaactcatgc acaacggcaa ggtgggtccg gtctcggacg
     2641 aacacaagga atacctgggc gacatcctca ccagcgcccg ccacctgttg cagctcatca
     2701 acgacgtgct ggacctcacg aaggtggagg ccggcaagat ggagttccgc cccgagctgg
     2761 tggacctgcc caaggtggtc tcggaggtga aggagatctt gaggggcctg gccgccccca
     2821 aacgcctcaa actggaggtg gcggtggacc ccgccctgga ccgggtggtc ctggacgcct
     2881 ccaagctcaa gcaggtgctc tataactacc tgtccaacgc catcaagttc acccccgagg
     2941 acggccgggt caccctgcgg gcctttcccg agggggcgga ccgtttccgc ctggaggtgg
     3001 aggacacggg catcggcatc caggaagggg acctgcacaa gctcttcatc gagttccaac
     3061 agctggacgc cgggtccgcc aagaaatacg ccggcaccgg gttgggcctg gccctcacca
     3121 agcgcatcgt ggaggcccag ggcggggaag tgggcgtgaa gagccgcccg ggcgagggca
     3181 gcgtcttctg ggcggtcttt ccccgggaat ggaaggggga gccgaatgcc cggtgaggag
     3241 atcctgatcg tggacgacaa cgccgtcaac ctgaagctgg cccgggtggt cctgatgggc
     3301 gcgggttacg ccgtgaagac cgcgggttcc gccgaggagg ccttgaaact cttggagacc
     3361 ctccatcccg ccctcatcct gatggacctg caattgcccg gcatggacgg gctggaactt
     3421 acccggcggc tcaaggccgc gcccgagacc cgctcggtca aggtcgtggc cttgaccgcc
     3481 tacgccatga agggcgacga ggaaaaggcc cgcgcggcgg gttgcgacgg ctatttggcc
     3541 aaacccatcg acacccagaa attctccgcc caagtggcgg ggtatttgga gagatgatgg
     3601 gttgcttt
//
LOCUS       DASTJJ010000134         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1733535, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000134 DASTJJ010000000
VERSION     DASTJJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733535"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFR02_01645"
     CDS             complement(<1..1298)
                     /locus_tag="VFR02_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9869188.1"
                     /translation="MAAFLSALGPLAARASVNGTTGNFPFPQNQDNPYGIHASVYNNQ
                     DVLDAYNKWYADCVTSSGAGGFLRVQRPNDSGLLLNSTVSEGIGYAMVIAVYMNDENL
                     FDNIWKYEQLHLDGNKLMNWYIDSSGGTAGSGAATDADEDMAWALLMASRQWGGSGTL
                     GGTYLNYAVSQINNLYATELNSGIPDGGDGFIPINPSYFAPAYYREFASAAGQTGWLT
                     VAANCYTVLNDNLGQGYGNAANGLVSAWCTSAGVSTATNAAPYDYQYDACRTPFRILQ
                     DYLWFGTAQSQAYDTLTSNFFSGVGAVSIVDGYYFNGAPHPHLPTLTVSQNPGFQSAA
                     FVGPAGCGGMVSHNYQAFVDQAYQDLVGSKLLVGGTYYDESWTVMSLLMMSGNFLDYR
                     LYTTPTPTPTLSAYSPLTIRVNAGGATFTDTAGAAWSADQ"
     gene            1553..2260
                     /locus_tag="VFR02_01650"
     CDS             1553..2260
                     /locus_tag="VFR02_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9869189.1"
                     /translation="MSLTFFVTGAGRGLGLAVTQTALAEGDRVVAGVRDPQGSDALKK
                     LKSQYGDLLSIVAYDALDEAKVRQAAAETAKLAPQVDILMNVAGISPPPPTAPLEEVD
                     LAKCREAFEVNVLGPLVTTRAFLPLLKKSPNPRVVHFTSGLASLAGKSEGGFYAYGVS
                     KTALNMLSRTMAFDLKRFNVVSVVLDPGWVQTDMGGPSAPLKPLESAGPIVRTAKGLT
                     LERTGQFLYNDGKQLNW"
     gene            complement(2263..>3172)
                     /locus_tag="VFR02_01655"
     CDS             complement(2263..>3172)
                     /locus_tag="VFR02_01655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869190.1"
                     /translation="VAWSFTNLGRVIEASGDFNGAKQMFREGMALDKEFGDRRALAWA
                     SLLMGQTCWALGDYEEAERHYREGERLYRESGDPRGSAWGLDLLGNLQVAQRRYEEAG
                     KFYERAMVILRKEGLNVQNRGWHAYHQGALRFFQGDYAGADKWLRQGLAAFRSVADPL
                     GQATCLTHLGESALERGRLREAEGCFARALELALPIRLMPHVADLLVAVAKLLKSRGD
                     ERSALVFLVAALSHPTCRRQTKDKIVLFTTKLESRFTTDEVQDAVQQAKASRLEDLAQ
                     AWLSGLHPARAREKARPRAPRRRKKR"
BASE COUNT          552 a         1027 c         1044 g          549 t
ORIGIN      
        1 tgctggtcgg cggaccaggc ggcccccgcg gtgtccgtga aggtggcccc cccggcattg
       61 acccggatgg tcaggggcga gtaggcgctc agggtgggcg tgggggtggg cgtggtatag
      121 aggcggtaat cgaggaaatt gccgctcatc atcaagaggg acatcaccgt ccaggactcg
      181 tcgtaatagg tgccccccac cagcaatttg gaccccacca ggtcctggta ggcctggtcc
      241 acgaaagcct ggtagttgtg ggaaaccatc cccccgcaac ccgccggccc cacgaaggcc
      301 gccgactgga aaccgggatt ttgagacacc gtgagggtgg gcaggtgggg atggggcgcg
      361 ccgttgaagt aatagccgtc cacgatggac accgccccca ccccggaaaa gaaattggag
      421 gtgagcgtgt cgtaggcttg ggactgggcg gtgccgaacc agaggtaatc ctgcaggatc
      481 cggaagggcg tgcggcaggc gtcgtattgg tagtcgtagg gggcggcgtt ggtggccgtg
      541 gagacccccg cgctggtgca ccaggccgag accagcccgt tggccgcgtt gccgtagccc
      601 tggcccaggt tgtcgttcaa gaccgtatag cagttggcgg ccaccgtcag ccatcccgtc
      661 tggccggcgg cggaggcgaa ttcccggtag taggcgggcg cgaagtagga ggggttgatg
      721 gggatgaagc cgtcgccgcc atccgggatg cccgaattga gctcggtggc gtagaggttg
      781 ttgatctggc tgaccgcgta gttcaggtag gtgccgccca aggtgccgct gccgccccac
      841 tggcgggagg ccatcaagag cgcccaggcc atgtcctcgt cggcgtcggt agcggcgccg
      901 gatccggccg tcccccccga ggagtcgata taccagttca tcagcttgtt gccgtccagg
      961 tgcagctgct cgtatttcca gatgttgtcg aagaggttct catcgttcat gtagaccgcg
     1021 atgaccatgg cgtacccgat gccctcggac accgtggaat tgagcagcag ccccgaatca
     1081 ttcgggcgct ggacgcgcag gaacccgccc gcgcccgagg aggtcacgca gtccgcgtac
     1141 catttgttgt aggcgtccaa cacgtcctgg ttgttataga ccgacgcgtg gatgccgtag
     1201 gggttgtcct ggttctgggg gaaggggaaa ttccccgtcg tgccgttgac ggaagcccgc
     1261 gcggcgaggg gtcccagggc ggacaagaag gcggccaaga caaggatttt tccaggcgaa
     1321 cgattcaagc ggagctccaa aaagaaggat ccccccaacg gtttccggag gatcggggaa
     1381 aggtcgagcg acgcatagtt taccgccctc tccgggacat ttcctctttt tcggccaaag
     1441 gggaccgagg cttccgcggt ttcacgtttc aaacgccccg cgtcttgcgg acaaaccccc
     1501 acgccgctac acttcacggg tcttgcttcc catcccgaac aacgaggcga tcatgtccct
     1561 gacttttttc gtgaccggcg ccggccgggg cttaggcctg gcggtgaccc agacggcgct
     1621 cgccgaaggc gaccgcgtgg tggcgggcgt ccgcgacccg caaggatccg acgcgttgaa
     1681 aaaactgaag tcccaatacg gcgacctatt gtccatcgtg gcctatgacg ccctggacga
     1741 agccaaggtg cggcaggccg ccgccgagac cgccaagctc gcgccccagg tggacatcct
     1801 gatgaacgtg gccggtattt ccccaccgcc ccccaccgcc ccgttggagg aggtcgacct
     1861 ggccaagtgc cgggaagcct tcgaggtgaa cgtgctgggc cccctggtga ccacccgggc
     1921 cttcctgccc ttattgaaga agtcccccaa cccccgggtg gtgcatttca cttccggatt
     1981 ggcgtcgctg gcgggcaaaa gcgaaggagg gttctacgct tatggggtct ccaagaccgc
     2041 actcaacatg ctcagccgca ccatggcctt tgatttgaag cggttcaacg tggtctcggt
     2101 ggtgttggac ccgggctggg tgcagaccga catgggcggc ccctccgccc ccctgaagcc
     2161 cttggaatcg gccgggccca tcgtgcgcac ggccaagggc ctgaccctgg agcgcacggg
     2221 gcagttcctc tacaacgacg gcaaacagtt gaactggtag ggtcagcgct tcttgcggcg
     2281 gcgcggcgcc cggggccttg ccttttcccg ggcgcgggcc ggatggaggc ccgaaagcca
     2341 ggcctgggcc aggtcctcca ggcgggacgc cttggcctgc tgcaccgcgt cctggacctc
     2401 gtcggtggtg aaccgggatt ccagcttggt ggtgaagagc acgatcttgt ccttggtctg
     2461 ccggcggcag gtgggatggc tcaaggccgc caccaaaaag accagcgcgc tgcgttcatc
     2521 gcctcgggac ttcaagagtt tggccaccgc caccaaaagg tccgccacgt ggggcatcag
     2581 ccggatcggc agggccagtt ccagcgcgcg ggcgaagcag ccctcggcct cccggaggcg
     2641 cccccgctcc agggcggatt cgcccaagtg ggtcaggcag gtggcctggc ccagcggatc
     2701 cgccaccgac cggaaggccg ccagaccctg gcgcagccat ttgtccgctc ccgcgtagtc
     2761 cccctggaag aaccgcaacg ccccttggtg gtaggcgtgc cagccccggt tctggacgtt
     2821 caatccttcc ttccttaaga tcaccatggc acgttcatag aactttccgg cttcctcata
     2881 gcggcgttgg gccacctgca ggttgcccaa aaggtccagc ccccaggccg acccgcgcgg
     2941 atccccgctt tcccggtaaa gccgctcccc ttcccggtaa tgccgctcgg cctcctcata
     3001 gtcccccaag gcccaacagg tctggcccat cagcagcgac gcccaggcca aggcgcgccg
     3061 gtcgccgaat tccttgtcca acgccatgcc ttcccggaac atctgcttgg ccccgttgaa
     3121 gtcgccggag gcctcgatga cccggcccag gttggtgaag gaccaggcca cc
//
LOCUS       DASTJJ010000135         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_755205, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000135 DASTJJ010000000
VERSION     DASTJJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755205"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1199)
                     /locus_tag="VFR02_01660"
     CDS             complement(6..1199)
                     /locus_tag="VFR02_01660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9869191.1"
                     /translation="MPSYVRVKLKRKEEDRLLAGHPWVFANEVDGIPKVLTPGALVEV
                     VTAEGAPVGRGVANPTSKILVRLLTRGFERELDGALIAERVERALAVREGMGEKAGTD
                     ALRLFFGEADGLPGVIADAFGRDVVLSCYSAGMKPFLPDVVSALEKKGYASVYEKSAG
                     ESVQKEGMAEVQEWRTAPGKTPFTFTEGRAKFTIDPGAGQKTGFYLDFREARRRVAAL
                     AQGRSVLDAFCYTGAAAVQAALGGAKEVLALDSSQSALEEALSNARLNGVEGKITFER
                     QDSFKIFRELKKAGRNFDGILLDPPPLAKSVHELPGGRAALKRMLGNALDLLNPGGWM
                     VVASCSHHFSWTVLEGVVREAVEESGRSFRLSERLSQPADHPMVVSIPETEYLRALVL
                     EEAGF"
     gene            complement(1243..>1688)
                     /locus_tag="VFR02_01665"
     CDS             complement(1243..>1688)
                     /locus_tag="VFR02_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869192.1"
                     /translation="KEKINRPNGFFLSDWINGLDKESKEARYKDLENQDHGPSVNNGQ
                     KAVVIVASSLVGLGGGLVMAYTLTDATTQDMFIGGALGLCAGIGIGALMMPNDYEVQQ
                     AGLGKGMPLAWENNVTFRQAFAQDPARLRTRAAFQPAYSVVSLKF"
BASE COUNT          267 a          590 c          544 g          287 t
ORIGIN      
        1 ccgtcctaga aacccgcctc ttccaacacc aaggcccgca ggtattcggt ctcgggaatg
       61 gacaccacca tgggatggtc ggcgggctgg ctgagccgct ccgacaggcg gaaggaccgg
      121 cccgattcct cgacggcctc ccgcaccacg ccttccaaca cggtccagga aaaatgatgc
      181 gagcaggagg ccaccaccat ccaacccccg gggttcaaga ggtccagcgc gttgcccaac
      241 atgcgcttca aggccgcccg gcccccgggc agctcgtgga cgctcttggc caggggcggc
      301 gggtccagca ggatgccgtc gaagttccgg cccgccttct tgagttccct gaaaatttta
      361 aagctgtcct gccgctcgaa ggtgatcttc ccctccacgc cgttcaggcg ggcgttggac
      421 agggcctctt ccagggcgga ttgggacgag tccagcgcca gcacttcctt ggcgccgccc
      481 agggccgcct gcacggcggc cgcgcccgtg tagcagaagg cgtccaacac ggaccggcct
      541 tgggccaggg ccgccacccg gcggcgggct tcccggaagt ccaagtagaa accggtcttc
      601 tggcccgcgc ccggatcgat ggtgaacttc gcccggccct cggtgaaggt gaagggcgtc
      661 ttgcccggcg ccgtgcgcca ttcctggacc tcggccatgc cctctttttg gaccgactcc
      721 ccggcgcttt tttcgtacac cgaggcgtag cccttttttt ccagggccga gaccacgtcg
      781 ggcaggaagg gcttcatgcc cgcgctgtag caggacagca ccacgtcgcg gccgaaggcg
      841 tcggcgatca cccccggcag gccgtcggcc tcgccgaaga agagacgcag ggcgtccgtg
      901 ccggcctttt cgcccatgcc ctcccggacg gccagggccc tttccacgcg ctcggcgatg
      961 agggcgccgt ccagttcccg ctcgaagccg cgggtcagga gccgcaccaa gatcttggac
     1021 gtcgggttgg ccaccccccg gcccacgggg gcgccctcgg ccgtgaccac ctccaccagg
     1081 gccccgggcg tgagcacctt ggggatgccg tccacctcgt tggcgaagac ccaggggtgg
     1141 cccgccaaca ggcggtcctc ctccttgcgc ttcaatttga cgcggacgta ggaaggcatg
     1201 gcatctcctg gggttgatgg gcgatggaac tcgggggaac ggctagaact tcaaggacac
     1261 gaccgaatag gcgggttgga aggccgcccg ggtcctcaag cgggccgggt cctgcgcgaa
     1321 ggcctgccgg aaggtcacgt tgttttccca ggccaggggc attcccttcc ccaaaccggc
     1381 ctgctgcacc tcgtaatcgt tcggcatcat gagcgccccg atgccgatgc cggcgcaaag
     1441 gcccagcgcg ccgccgatga acatgtcctg ggtcgtggcg tccgtcaggg tataggccat
     1501 gaccagcccg ccccccagcc ccaccaggct ggaggccacg atgaccacgg ccttctggcc
     1561 gttgttcacc gaggggccgt ggtcctggtt ctccaggtcc ttatagcggg cttccttgga
     1621 ttccttgtcc aggccgttga tccagtcgct gaggaagaag ccgttgggcc ggttgatctt
     1681 ttccttgg
//
LOCUS       DASTJJ010000136         1868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_511102, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000136 DASTJJ010000000
VERSION     DASTJJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511102"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1868
                     /locus_tag="VFR02_01670"
     CDS             <1..>1868
                     /locus_tag="VFR02_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869193.1"
                     /translation="YLDKLSGDSQTNPTDYQEAVQRSTDRKLSGFLRAKYAYHAVRMA
                     VLAEQWDLGISNYEKLFAPLGVTSLVRYEAMGWWARALHMSDQNGKAVSIYAEMFDQC
                     PAMRDEARHSLYMIDEGGSQIGDLADRLKPGHRKVTALYLEHLIGGRDYSASTLSKVT
                     ELGPREIQVEMMFLQMVQDIEKDFFVTECPNLLGETDLVLPKNKFLRFLVKTANSKPG
                     FVDLIPVCEKAAANPKTRRPAFWRWGASYLALLSGDVKTARADLNQAKALGAKDASLA
                     PTLHLQETLVEMAESPGTFSPALQERLCEDLDWAKEENPGPATYDQLERGPRHPMNPE
                     LENTLWALAVQKYIYMGDWPHACLLASAAAPPPYMSDGQTDMSLFLDFSTPEELSKLK
                     RLLEAKRMSPLSKLLPGFGHLTPLEALALGRTSLVPKDLDLLIALRKANRLDFAGALS
                     DIEGLEAKAPSYLAAEVKPVTEFYASGAVTVYQNTSIPAKQHIDFSHDLKRITGDPQT
                     WDLDLERYLKLMTKFSSDLDKGRKGDKALEAKSAFEIGRILASEGMTGWPEFRCGQDY
                     SDGESYRLHTESSPIYFMGAPLEAERKKRLEQYNRDTPDFREVASRYFQEVVDTKV"
BASE COUNT          382 a          540 c          570 g          376 t
ORIGIN      
        1 atacctggac aagttgagcg gtgattccca gaccaatccc acggattatc aggaggcggt
       61 ccaaaggtcc actgaccgca agttgagcgg gttcctgcgg gccaaatacg cctatcacgc
      121 ggtccgcatg gcggtcctcg cggagcagtg ggacctgggc atctcgaatt acgaaaagct
      181 tttcgccccc ttgggcgtga cgtcactggt gcggtatgag gccatgggtt ggtgggcgcg
      241 ggcgcttcac atgtccgatc aaaacggcaa agccgtttcg atctacgccg aaatgttcga
      301 tcaatgcccg gccatgcgcg atgaggcgcg ccattccttg tatatgatcg atgagggtgg
      361 gtcgcagatc ggcgacctgg cggatcgcct caagccgggg caccggaagg tcaccgcgct
      421 ttacctggag cacctgatcg gaggacggga ttattcggcc tccaccctct cgaaagtgac
      481 cgaactgggc ccccgggaga tccaggtgga gatgatgttc ctccagatgg tccaggacat
      541 tgagaaggat tttttcgtca ccgaatgccc gaatcttttg ggcgagacgg acctggtctt
      601 gcccaagaac aagttcctgc ggttcctggt caagaccgcc aactcgaagc cgggtttcgt
      661 cgatctgatc cccgtctgcg aaaaggccgc cgccaacccc aagacacgcc ggccggcctt
      721 ttggcgctgg ggcgcttcct acttggcttt attgtccggg gacgtcaaga cggcccgagc
      781 ggacctgaac caggccaagg cgctgggggc gaaagacgcc tcgctggcgc ctacgttgca
      841 cctgcaggag accttggtgg agatggcgga aagccccggg actttttccc cagccctcca
      901 ggaacggctt tgcgaggacc tggattgggc caaggaagaa aacccggggc cggccaccta
      961 tgaccaactt gagaggggcc cgcgccatcc gatgaacccc gaactggaga acaccctctg
     1021 ggctttggcc gtccaaaagt acatttacat gggggattgg ccccatgcct gcctgttggc
     1081 ctcagcggcg gcgccgcctc cctacatgtc cgatggccag accgatatga gccttttcct
     1141 cgatttttcc accccggagg aattgtccaa gttgaagagg ctcctggaag ccaagcggat
     1201 gagccccttg agtaaattat tgccgggttt tgggcacttg acgcccttgg aggccttggc
     1261 cttgggccgg acctcgttgg tccccaagga cttggacctt ttgatcgcgt tgcggaaggc
     1321 gaaccggctt gatttcgccg gcgccctttc ggacatcgaa ggcctagagg cgaaggcccc
     1381 ttcctactta gcggcggaag tgaagccggt gacggaattt tacgcgtcgg gagccgtcac
     1441 cgtttatcaa aatacctcga tccccgccaa gcagcatatc gatttcagcc atgatctcaa
     1501 gcggatcacc ggcgaccccc agacctggga cctggacctg gaacgttatt tgaagctcat
     1561 gaccaaattc tcctcggacc tcgataaagg ccgcaaaggc gacaaggcgc ttgaggcgaa
     1621 gtcggctttt gagatcgggc ggatcttggc cagcgagggg atgaccggat ggccggagtt
     1681 ccgttgtgga caggattatt ccgacggcga aagctatcgt ctccacacag agtcttcccc
     1741 gatttacttc atgggcgcgc ccttggaggc cgaacggaaa aagcggttgg aacagtacaa
     1801 ccgggacacg cctgattttc gtgaggtcgc cagccggtat tttcaggaag ttgtggacac
     1861 gaaggtcg
//
LOCUS       DASTJJ010000137         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_511122, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000137 DASTJJ010000000
VERSION     DASTJJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511122"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="cysD"
                     /locus_tag="VFR02_01675"
     CDS             complement(<1..897)
                     /gene="cysD"
                     /locus_tag="VFR02_01675"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET9869194.1"
                     /translation="MSTAMSVKTLSHLQQLEAESVFIIREVVAEFKNPVMLYSVGKDS
                     SVMLRLAQKAFAPGKIPFPLLHVDTGYKFPEMYEFRDQLVKELGAKLLVERNEEAIAG
                     GASPFKYGVQKCCGLLKTQGLLDGLAKHGFDAAFGGARREEEKSRAKERVYSFRDAFG
                     QWDPKNQRPELWDLYNSKINEGESIRVFPLSNWTELDIWLYIEMEKIPVVPIYFTHTR
                     KIIKRKSLLIPFAPHIPILPGDEVQDMRVRFRSLGCMSCTGAVRSEKEVTDLRGIIED
                     LITTRKSERENRIIDHGSDSSME"
BASE COUNT          202 a          352 c          360 g          235 t
ORIGIN      
        1 ttccatggag ctgtcggacc cgtggtcgat gatgcggttc tcgcgctcgc tcttgcgggt
       61 ggtgatgagg tcctcgatga tgccccgcag gtccgtcact tccttctcgg accgcacggc
      121 gcccgtgcag gacatgcagc ccagcgaccg gaaccgcacc cgcatgtcct gcacctcgtc
      181 cccaggcagg atcgggatgt ggggcgcgaa ggggatgagc aggctcttgc gcttgatgat
      241 cttgcgggtg tgggtgaaat agatgggcac cacggggatc ttctccatct cgatgtagag
      301 ccagatgtcc agctccgtcc agttggagag cgggaagacc cggatggact ccccctcgtt
      361 gatcttggaa ttatagaggt cccagagctc gggcctctgg ttcttggggt cccattggcc
      421 gaaggcgtcc cggaaggaat agaccctttc cttggcgcgg gatttttcct cttcccgccg
      481 ggccccgccg aaggccgcgt cgaaaccgtg cttggcaagc ccgtccagca ggccctgggt
      541 cttcagcagg ccgcagcatt tctgcacgcc gtacttgaag gggctggcgc cgcccgcgat
      601 ggcctcctcg ttgcgttcca ccagcagctt ggcgcccagc tccttcacca actggtcgcg
      661 gaactcgtac atctcgggga acttgtagcc cgtgtccacg tgcagcagcg ggaaagggat
      721 cttgcccggc gcgaaggcct tctgggccag gcgcagcatg acgctggagt ccttccccac
      781 cgagtagagc atgaccgggt tcttgaactc ggccaccacc tcccggatga tgaagacgct
      841 ttcggcctcc aattgctgga ggtgggatag ggttttgacg gacatcgcgg tggacatgcg
      901 gttcgggcct tttaggggtt attcttgggc ttccccccgg aaagttccag gggctgggtc
      961 aaaagtccca taaaaataac cggtttcggc cccaaactca acgcaaaagg ccgccggtca
     1021 gatcggaaag acgtggaagg ccgtgatggc ggcgaaaacc tccatccctt ccttaaaacc
     1081 gctggccagg gccacgccct tgggcagttg ggccgagatg accttgtcct cgccgatatc
     1141 cagcttgta
//
LOCUS       DASTJJ010000138         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1612172, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000138 DASTJJ010000000
VERSION     DASTJJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612172"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="jag"
                     /locus_tag="VFR02_01680"
     CDS             complement(<1..1005)
                     /gene="jag"
                     /locus_tag="VFR02_01680"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HET9869195.1"
                     /translation="MRPHSNQNSGRGDQRHSGQGNGNRHSSGQKPPYGGNREQRERRE
                     FRGADRADRGRGDREHRFGGGNGQQRSHGFRPDRDRNQSFRDRDRDRHRGHSHAPADR
                     GPSVPRGPRSSWVEVTGRGAEDAVQEALRRFNVGRNDLNIEVVDEGSRGFLGIGSKPA
                     KVKVALKPGAVMPFAEGVLTRLLRAMGLPDTVQGQKDAEGNPVLNIQGGSSGTLIGRH
                     GHTLESLQYLVSKIVQRMTGDEHVMIVVDVENYLERQREKLKELALGLADKARETGTE
                     VPMRPMNSKDRRVVHLALKDHEHVTTESRGEGLRRRVVIVPKVKAAAAVAAVPAEGSA
                     E"
     gene            complement(1097..2710)
                     /gene="yidC"
                     /locus_tag="VFR02_01685"
     CDS             complement(1097..2710)
                     /gene="yidC"
                     /locus_tag="VFR02_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9869196.1"
                     /translation="MENQNRNLILFIVISIAIYFVCLRFLPQPGFPPAGQTATTAATP
                     EPTPALAAPAPKASSLALAPVRASVPAASVTVSTDDYEAVFSNEGAVLTSFRLKQYSN
                     RITGKPLQMVNPAPGHPKPFTLEDASLPDLSGQRFEIKGTSKTLAKGQTADLVFRTQT
                     AGGTRVEKKFHFEGGSYLIGFDVALAQAHGQLAASPLGVAWADTLGVEDDMGTQSRVT
                     GYRVATLAGGHLDSQSAKKSQESTDIPAPVRWTALANQYFLAALVPDPASGGASAKIV
                     RDFHTFLPPTADNPTPGLSPKEYAPRPILEFPGQALSAGQGFERRGQVFLGPQNYYLL
                     KGLKLHLEQVVDFGWFGFISIYMLQLLCWFEGFCHNWGLAIILLSIVVKLVLWFPTRS
                     SYKSMYQTQQKMREIQPKVESLKRKYADDKAKLNQETQDLYAKAGINPLGGCLPLFLQ
                     MPIFFALYATLSHSIELRGASFLWIKDLTLMDPLHVLPLFMGASMIVQQRVSGQAAAN
                     APGQQRVMMWIFPVVLTAVSFQWPSGLLI"
BASE COUNT          457 a          885 c          953 g          512 t
ORIGIN      
        1 ctcggcggat ccttcggcgg ggacggcggc cacggcggcc gcggccttga ccttgggcac
       61 gatcaccacc cggcggcgca ggccttcgcc ccggctctcg gtggtcacgt gctcatggtc
      121 cttgagcgcc aagtgcacca cccggcggtc tttggagttc atggggcgca tgggcacctc
      181 ggtcccggtc tcccgggcct tgtcggccaa tcccaaggcc agttccttga gcttctcgcg
      241 ctggcgctcc aggtagttct ccacgtccac cacgatcatc acgtgctcat cgcccgtcat
      301 gcgctggacg atcttcgaca ccagatactg cagcgattcc agcgtgtggc cgtgacggcc
      361 gatcaaggtg ccgctggagc ccccctggat gttcagcacc gggttgcctt cagcgtcctt
      421 ctgcccctgg acggtgtccg gcaatcccat ggcccgcagc aggcgggtca gcacgccctc
      481 ggcgaagggc atgaccgccc cgggcttcaa ggccaccttg accttggccg gcttggaacc
      541 gatgcccagg aacccgcggg acccttcgtc caccacttcg atgttcaggt cgttgcggcc
      601 cacgttgaaa cggcgcaggg cctcctggac ggcgtcctcg gcgccacggc cggtcacttc
      661 gacccaggaa ctgcgcgggc cgcgggggac cgaaggcccc cggtccgccg gggcgtggga
      721 atggccccga tggcggtcgc ggtctcggtc tcggaatgac tggttccggt cccggtccgg
      781 acggaaaccg tggctgcgct gctggccgtt gccgccgccg aagcggtgct cccggtcgcc
      841 ccggccccgg tcggcccggt cggccccgcg gaattcccgg cgttcccgtt gctcccggtt
      901 cccgccgtag ggcggcttct ggcccgagga atggcggttc ccgttccctt ggccgctatg
      961 gcgttggtcg ccccggccgc tgttctgatt ggaatgtggt ctcataaaat tatgcctcgt
     1021 ctttcttctt ttgctttttg atctcccggt tcacttcctt ttgctgcacg atcgacagca
     1081 ggttcgtcac cgcccactag atcaacagcc cggagggcca ctggaaggag acggccgtga
     1141 gaacgaccgg aaagatccac atcatgactc tttgttgacc aggggcgttg gccgccgcct
     1201 gtccggagac cctctgttgg acgatcatgg aagcgcccat gaacaggggt aacacgtgca
     1261 acggatccat caaggtcagg tccttgatcc agaggaacga ggcgccgcgc agctcgatgc
     1321 tgtgcgacaa cgtggcgtaa agcgcgaaaa agatgggcat ctgcaggaac aggggcaggc
     1381 agccgcccag cgggttgatg cccgccttgg cgtagaggtc ctgggtttcc tggttcaatt
     1441 tggccttgtc gtcggcgtac tttcgcttga gcgattccac cttgggctgg atctcgcgca
     1501 tcttctgctg ggtctggtac atcgacttgt agctggagcg cgtggggaac cacagcacca
     1561 gcttcaccac gatggacagg aggatgatgg ccaggcccca attgtggcag aagccctcga
     1621 accagcagag cagttggagc atgtagatgc tgatgaaacc gaaccagccg aaatccacca
     1681 cctgttccag gtggagcttc aaccccttga gcaggtaata gttctggggg cccaggaaga
     1741 cctggccccg gcgctcgaac ccctggcccg ccgaaagggc ctggcccggg aattccaaga
     1801 tgggccgggg cgcgtattcc ttggggctca ggcccggggt ggggttgtcc gccgtgggcg
     1861 gcaggaaggt atgaaaatcc cggacgatct tggccgaagc cccgcccgag gccggatcgg
     1921 gaacgagcgc cgccaggaaa tattggttgg ccagggcggt ccagcgcacg ggggcgggga
     1981 tgtcggtgct ttcctgcgat ttcttggcgg attgggaatc cagatggccg ccggccaggg
     2041 tggccacccg gtagcccgtc acccgggact gggtgcccat atcgtcctcc acgcccagcg
     2101 tgtcggccca ggctacgccc aggggcgaag ccgccaactg cccgtgggct tgggccaggg
     2161 cgacgtcgaa accgatcagg tagctgccgc cctcaaagtg gaatttcttt tccacccgcg
     2221 tgccgccggc ggtctgggtg cggaagacga ggtcggcggt ctggcccttg gccagggtct
     2281 tggaggttcc cttgatctcg aagcgctgtc ccgagaggtc gggcagggac gcgtcctcca
     2341 acgtgaaggg cttgggatgg cccggcgccg ggttcaccat ctggaggggc ttgcccgtga
     2401 tgcggttgga gtattgcttc agccggaaac tggtcagcac ggcgccttcg ttcgagaaga
     2461 ccgcctcgta atcgtcggtg gagacggtca cgctggcggc gggcacggaa gcgcggaccg
     2521 gggccagggc gaggctggag gccttggggg cgggcgccgc cagggccggc gtgggttcgg
     2581 gcgtcgccgc cgttgtcgcc gtttggcccg cgggcgggaa gccgggttgg ggcaggaacc
     2641 gcagacagac gaaatagatc gcgatggaaa tgacgatgaa aagaatgagg ttccggttct
     2701 ggttttccaa gcaaagcccc cgttaaagaa ttacgaaacg gggtggaacc ctccggccga
     2761 caggggatgg caacggctca aacgctttaa acccagccca aggccct
//
LOCUS       DASTJJ010000139         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_22051, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000139 DASTJJ010000000
VERSION     DASTJJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22051"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFR02_01690"
     CDS             complement(<1..700)
                     /locus_tag="VFR02_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869197.1"
                     /translation="MKLGKVLGGLALAAWPVGAWAQNADLDALLAQVPAPPVKIGAAD
                     LRWKPGDDVNGSQAHFDKDATALLAKLDQLEKAEQTGQFSYGGDSAEEMQKKMASMSQ
                     QEKMAYAMQLAKKAQADQRAAYQGSNARVLAQAQQNDVDLVHKGLASEKAAKEMSDLD
                     AQYNAKFDALLTRMLDDVKQCPTVTQGEASGPQKACVQGILDRYGKDYRALAEAKMAA
                     YHAIYLEFKKGATAE"
     gene            complement(784..1461)
                     /locus_tag="VFR02_01695"
     CDS             complement(784..1461)
                     /locus_tag="VFR02_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9869198.1"
                     /translation="MLEFFDAIQAGDLPKVQALFHKDPKLADAKNGQGLSAVLWARYH
                     DRMDVLEFLLKSKEQPLTIFEAAATGNRKTAHYLDQLDKGLVNSYSVDGFTPLQLACF
                     FATAETVKHLITLGADVRPAAKHASGLTALHAAVANRDGGEALEIVKALLILGADPKA
                     RQAGGLTPLHAAAARGSWELALMLLLFGADPRSKDDEGRTPRQVAEDKHYQALLELLD
                     KAVPARS"
     gene            1584..3461
                     /gene="hemE"
                     /locus_tag="VFR02_01700"
     CDS             1584..3461
                     /gene="hemE"
                     /locus_tag="VFR02_01700"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET9869199.1"
                     /translation="MASFESRMAKEMEELIRRNGGIPRVAPSMREIPLTDNPQAFEFF
                     ERLQAGGFDLLVLLTGVGTRALLEALGARYPLEEVLAPFKRVSIAVRGPKPVQALGVF
                     GLRPDWVAPEPNTWRELLSLLDAQRPVKGLRVAVQEYGVSNAAFLEALRDRGAAEVVS
                     VPVYRWALPEDTAPLVQLLDTIRSGGAQVALFTSANQVHTIFQLEREQGREAELKEAF
                     QHMVVGSVGPVASETLESYGIRVDLEPSHPKMGFLVKELAAKSAELLDEKARHFLSAP
                     RPRRDPLADSLFLKACRREPVDRTPLWIMRQAGRYLPQYRSVRAKVSFLELCKTPELA
                     AEVTVTAQETLGVDAAILFADILLIAEPLGFKLEFAPSGGPVIRNPFRSASDLKRLRP
                     VDPSTDLAYVLQAVSLIRSQLQPGIPLIGFAGAPFTLASYLIEGGGSKDHARVKALLG
                     DEAAWSGLMDRLVTATTAYLTAQAAAGAQALQLFDSWVGILTPEQFQTRVLPYLKKLI
                     AGLPAGVPVIYFGTQTAPLFPWIKETGATVVGVDWRMPLDEAWKRLEGLAVQGNLDPE
                     VLLTDPATVRRETEKILTLAAGRPGHIFNLGHGILPHTPLENVRAMIETVKAHPTRPK
                     G"
     gene            complement(3458..>4431)
                     /locus_tag="VFR02_01705"
     CDS             complement(3458..>4431)
                     /locus_tag="VFR02_01705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9869200.1"
                     /translation="VFFVVSGFLITKTIAQGRGGLFKPDIRDFYVRRIGRILPLLGLS
                     IAAGIGAIFFIPASAPHLSYCLGGQQSRYEPLFWVSIPFFSFNWFRAFISRHDYGLHW
                     DVLWSLSVEEQFYLFYPYCLRKIGSKNGLYLFLGSWVLLGLAVRLGGVLFFPDEKYWN
                     LGSFANFDDIAMGCLLYLVRERHGKELGREKWLCRFLCLSGLFMLLGAYGATPTDLDY
                     GRRIFGNTLVGGGVFLLLLGGLQLDGFKSGFWAPLRWPGKLSYGAYLLHPLVLFFLWP
                     VLSGFSWLPAFLLFSGAVSGIAWVSYRSFEVPINHWVRSRFKGAARD"
BASE COUNT          848 a         1470 c         1355 g          758 t
ORIGIN      
        1 tctcggccgt ggcgcccttc ttgaactcca ggtaaatggc gtggtaggcg gccatcttcg
       61 cttcggccag ggcccggtaa tccttcccat accggtccag gatgccctgc acgcaggcct
      121 tctggggccc gctggcttcc ccctgggtca ccgtggggca ctgcttcacg tcgtccagca
      181 tccgggtcaa caacgcgtcg aacttggcgt tgtattgggc gtccaggtcc gacatttcct
      241 tggcggcttt ttcgctggcc aagcccttgt gcaccaggtc gacgtcgttc tgctgggcct
      301 gggccaaaac ccgggcgttg gagccctggt aggccgccct ctggtcggct tgggcctttt
      361 tggccaactg catggcatag gccatttttt cctgttggct catggaggcc atcttcttct
      421 gcatttcctc cgcgctgtcc cccccgtagc tgaactggcc ggtctgttcg gccttttcca
      481 gttggtccaa cttggccaac agggccgtgg cgtccttgtc gaaatgcgcc tggcttccgt
      541 tgacgtcgtc gcccggtttc caccgcaggt ccgccgcgcc gatcttgacc ggcggggccg
      601 gcacctgggc caacaaggcg tcgagatccg cgttctgggc ccaagccccc accggccagg
      661 ccgccagcgc caacccaccc aagactttcc cgagtttcac gaccgcctcc tcacggcagg
      721 atgaaagacg gggaattttg tcctaaaaaa tgatggttgg caatgggctt tcgggatgaa
      781 agatcaagaa cgggcgggga cggccttgtc gaggagctcc agcagggcct ggtaatgctt
      841 gtcctcggcc acctggcggg gcgtgcggcc ctcgtcatcc ttggaccgcg gatcggcccc
      901 gaaaagcagc agcatcaagg ccagttccca gctgccccgg gccgccgccg cgtgcagggg
      961 cgtgagcccc cccgcctggc gggccttggg gtcggcgccc aggatgagca gggccttgac
     1021 gatctccagg gcttcccccc cgtcccggtt ggccaccgcc gcgtgcaggg ccgtcagacc
     1081 ggaggcgtgt ttggccgccg ggcgcacgtc cgcccccagc gtgatgaggt gcttgaccgt
     1141 ttccgcggtg gcgaaaaagc aggccaactg gaggggggtg aagccgtcca ccgagtaact
     1201 gttcaccagg cccttgtcca gctgatccag gtaatgggcg gtcttgcggt tcccggtggc
     1261 ggcggcctcg aagatggtga ggggctgttc cttcgacttc aacaggaact ccagcacgtc
     1321 catccggtcg tggtagcggg cccacaggac ggcggacagg ccctgcccgt tcttggcgtc
     1381 cgccagtttg gggtccttgt ggaaaagggc ctggaccttc ggcaggtcgc ccgcttgaat
     1441 ggcgtcgaaa aattccagca tggtcggccg gctccggcta tggatttagg gtttgccttg
     1501 gctatactgg cggcgtggaa accactttaa accaaagccc catggaaaga caggatgaaa
     1561 agcggggatt ccaaggcctg accgtggcct ccttcgagag ccgcatggcc aaggagatgg
     1621 aggaactcat ccgccgcaac ggcggcattc cccgggtggc gccttccatg cgggagatcc
     1681 ccctcacgga caatccccag gccttcgaat ttttcgagcg gctccaggcc ggcggcttcg
     1741 acctgttggt gctgctcacc ggcgtgggca cccgggcgct cctcgaggcc ctgggcgccc
     1801 gctacccact ggaagaggtc ctggcgccct tcaagcgggt gtccatcgct gtgcgggggc
     1861 ccaagccggt ccaggccctg ggggtcttcg gcttgaggcc cgattgggtg gcgcccgaac
     1921 ccaacacctg gcgcgaactg ctttcactcc tggacgccca gcggcccgtg aagggcctgc
     1981 gggtggccgt gcaggaatac ggcgtgtcca acgccgcgtt cctggaagcg ctgcgggacc
     2041 gcggcgccgc cgaggtggtc agcgtgcccg tttaccgttg ggccctgccc gaagacaccg
     2101 cccccctggt ccaactgctc gacaccatcc ggtccggcgg cgcccaggtg gcgctcttca
     2161 ccagcgccaa ccaggtccat accatcttcc aactcgagcg cgaacaaggg cgggaagccg
     2221 aattaaagga agccttccaa cacatggtgg tcggctcagt gggtccggtg gccagcgaga
     2281 cgctggaaag ttacggcatc cgggtggacc tggaacccag ccatcccaag atgggttttt
     2341 tggtgaagga actggccgcc aaaagcgcgg agttgctgga tgagaaggcc cggcatttcc
     2401 tttccgcccc ccgtccccgg cgggatcccc tggcggacag cctgttcctc aaggcctgcc
     2461 ggcgggaacc ggtggaccgc acgccccttt ggatcatgcg ccaggccggc cgttacctgc
     2521 cccaataccg ctccgtgcgg gccaaggtgt cctttttgga gctttgcaag acgcccgaac
     2581 tggccgccga ggtcaccgtc accgcgcagg aaacgctggg cgtggacgcc gccatcctct
     2641 tcgccgacat cctgctcatc gccgaacccc tgggcttcaa gctggaattc gccccctcgg
     2701 gcggccccgt catccggaac cccttcagat cggcctccga cctgaaacgc ctcaggcccg
     2761 tggatccctc gacggacctg gcctacgtgc tgcaggcggt ttccttgatc cgctcccagc
     2821 tccagcccgg gatcccgctc atcggcttcg ccggggctcc cttcaccctt gcctcttacc
     2881 taatagaagg cggcggttcc aaggaccatg cccgggtcaa ggcgcttttg ggggatgaag
     2941 ccgcctggag cgggctgatg gaccgcctgg tcacggccac caccgcctac ctgacggccc
     3001 aggccgccgc cggggcccag gccctgcaat tgttcgattc ctgggtgggg atcctgaccc
     3061 cggaacaatt ccagacccgc gttctccctt atttaaagaa actcatcgcc ggacttcccg
     3121 ccggggtgcc ggtcatttat ttcggcaccc aaaccgcgcc ccttttcccc tggatcaaag
     3181 agaccggggc caccgtggtg ggggtcgact ggcggatgcc cctggacgaa gcctggaagc
     3241 gactggaagg gttggcggtg cagggcaacc tggacccgga agtgctcttg accgacccgg
     3301 cgaccgtgcg ccgggagacc gaaaagatcc tgacgctggc cgcgggccgg cccgggcaca
     3361 tcttcaacct gggccacggc attttgcccc acacccccct ggagaacgtg cgggccatga
     3421 tcgagacggt gaaggcccac ccaacccggc ccaaaggcta atcccgggca gcgcccttga
     3481 agcggctgcg gacccaatga ttgatcggca cctcaaagga ccgataagag acccaggcaa
     3541 tgcccgaaac ggcgcccgag aacagaagaa aagccggcaa ccaggaaaaa cccgatagaa
     3601 ccggccatag aaaaaaaagc accaggggat ggagcaggta agccccatag ctcaactttc
     3661 ccggccagcg gagcggggcc caaaatcccg acttaaaacc atccaattga aggccgccca
     3721 gcaggagcag gaagaccccg ccgcccacca acgtattgcc gaagatacgg cgtccataat
     3781 cgaggtcggt cggcgtcgcc ccataagcgc ccagcaacat gaaaaggccc gacaggcaca
     3841 agaaccggca caaccacttc tcccggccga gctccttccc gtggcgctcg cggaccagat
     3901 atagaaggca tcccatggcg atgtcgtcga agttcgcgaa agaaccaagg ttccaatatt
     3961 tttcatccgg gaagaagagc acgcccccca agcggacggc caggcccaac aacacccaag
     4021 aacccaggaa aagataaagc ccgtttttcg acccgatctt ccgcaggcaa tagggataaa
     4081 aaagatagaa ctgttcctcc accgaaaggg accaaaggac gtcccaatgc agcccataat
     4141 catgccgact gatgaaggcg cggaaccagt tgaatgaaaa aaacgggatc gaaacccaga
     4201 acagcggctc atagcggctt tgttggccgc ccaggcaata ggacaagtgg ggcgccgaag
     4261 cgggaataaa aaagatggcc ccaatgccgg ccgcgatcga caagcccaac aatgggagga
     4321 tgcgtccgat ccggcggaca taaaaatccc tgatgtcggg cttgaaaagc cctccccgcc
     4381 cttgcgcgat ggtcttggtg atcaagaacc ccgagaccac gaaaaagacc t
//
LOCUS       DASTJJ010000140         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_511394, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000140 DASTJJ010000000
VERSION     DASTJJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511394"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1144)
                     /locus_tag="VFR02_01710"
     CDS             complement(<1..>1144)
                     /locus_tag="VFR02_01710"
                     /inference="COORDINATES: protein motif:HMM:NF036827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9869201.1"
                     /translation="TCSILHVLTPGVQMFSPSQMRDGVEWGGKLGSALGDKLRSILTG
                     FGTVLVGLLVLVLSAWCLEVEDLVLKAAQVLLGRASKGAEWAKTHGFQAAFRLYEGLS
                     KRIEGLNARRSEKKAARAAREKLKVERQAFTSVFSGEAAAEPEKTVPEPARSERPAPA
                     RRVPAPPPPVEGEEDQVAAALAEARGGEGMTLKQKMEAADIDLGAEGDAQGAAAPEAA
                     EEKPKRIIRQWQLPAVHLLKTVEEAREQGFDDFESVSRTIIETLDNFNVEARVVGVNP
                     GPTVTQYEVQPAAGVKINRISSLADDLALALQCGQVRVVAPIPGKAAVGIEVPNLKGR
                     VVTLKELIAQDDFTSSSKKLLVALGKDIVGATCLANLKEMPHVLIAG"
BASE COUNT          169 a          411 c          364 g          200 t
ORIGIN      
        1 ccccgcgatg agcacgtggg gcatttcctt caggttggcc aggcaggtgg cccccacgat
       61 gtccttgccc agggccacca gcagtttttt ggaggaggag gtgaaatcgt cctgggcgat
      121 caattccttc aaggtgacca cccggccctt gaggttcggc acctcgatgc ccaccgcggc
      181 cttgcccggg atgggcgcca ccacccgcac ctggccgcat tggagggcca gcgccaggtc
      241 gtcggccagg gacgagatgc ggttgatctt cacgcccgcc gccggctgga cctcgtactg
      301 ggtcaccgtg gggccggggt tgacgcccac cacccgggct tccacgttga aattgtccag
      361 ggtctcgatg atggtgcggc tcaccgactc gaaatcatcg aagccctgtt cccgggcctc
      421 ctccacggtc ttcaacaggt ggacggcggg caactgccac tggcggatga tccgtttggg
      481 tttttcctcg gccgcttcgg gggcggcggc cccttgggcg tccccctccg cccccaggtc
      541 gatgtcggcg gcctccatct tttgcttcag ggtcatgcct tccccgcccc gggcctccgc
      601 cagggcggcg gccacttggt cctcttcccc ttccaccggc ggggggggcg cggggacccg
      661 gcgggcgggg gccggccttt ccgaacgggc tggctccggg acggtctttt ccggctcggc
      721 ggcggcctcc cccgaaaaga ccgaggtgaa agcctggcgt tccaccttca atttttcccg
      781 ggccgcccgg gcggccttct tttcggaacg gcgcgcgttc aacccctcga tccttttgga
      841 aaggccctca taaagccgga aagcggcctg gaaaccgtgg gtcttggccc attcggcccc
      901 cttggacgcg cggcccaaca ggacctgggc cgccttcaag accaagtcct ccacctccag
      961 gcaccaggcg gacagcacca agaccaacag ccccaccagc acggtgccga aacccgtcag
     1021 gatgctgcgc agcttgtcgc ccagggccga ccccagcttg cccccccatt ccaccccgtc
     1081 gcgcatctgg gagggcgaga acatctgcac cccgggggtg agcacatgga ggatcgagca
     1141 ggtg
//
LOCUS       DASTJJ010000141         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_144782, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000141 DASTJJ010000000
VERSION     DASTJJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144782"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VFR02_01715"
     CDS             <1..1568
                     /locus_tag="VFR02_01715"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9869202.1"
                     /translation="LAPLGAQETGGTVSPFDLGGSARTLGMGGVGVALSGDGASFLRN
                     PAVLATLGEQELLTFHSPLLADASYDALGFVLPVASHASFGAAVERVSVTGIQETTDS
                     VSPTGTLADEQLQGVLGYGLEIWDGLSLGGNVKYDRQTLGSYQDGGMGADVGLLYRLG
                     RSAKDYEGFGLANMTLGFSVSNLVAPESHLFQLADQPARVYQPALSYRYESADQSNAL
                     WLAVEGRVVENGASQVLAGLEYGWNQTIFGRVGYDGVGPTAGAGLRFEGLEFDYAFNQ
                     RDLGALQRFSLTYSFNPYRDPQAGQKMKTMKFIAQSYDRENEYDAAIQAWKSVADEFP
                     DDGDPPAAIRSLQDRRQKAVRSDLDEGRQALEANNLDRAIRFLAHGLSLDPTNPQIKK
                     ILGRVNHEMVLTSNYMRGTEAYSREDYKTAVRYLGAVYDLDPGYRDTAFLYHDAQSHY
                     MPLESMDREAGDHYALGVNAYMAGQYAQAIDEWKKVLEKEPKNYLVRRNIEEARQLLQ
                     AQGDAGAAKPPKD"
     gene            1571..4228
                     /locus_tag="VFR02_01720"
     CDS             1571..4228
                     /locus_tag="VFR02_01720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9869203.1"
                     /translation="MDLKKFIPDLTELIPFLRPHRRVFRLEVPAKEIELLTIKDFIQR
                     VCDAAGCSSKETSSVKLAIDEACSNIVRHAYQGREDGKIILEAGIGVKDIRIAITDFG
                     KSFDFRKIEAPDLNHYVDIGKKGGLGIFLIKKIMYKVDYRTKDGRNDLILHRRLSQAP
                     PAAALAKGQRTLSISSRFTIMSTGLIFLLIVLLYFFLNFRNQNFILSQQRDKALSLTQ
                     AMAAQAGESVVRKDDLGLDHLIGQVVRADGGRVFDYVFVVNQDDKFLAHSDLKQVFQE
                     YHRPRTVKPVRQGGVRVFEVKTPQGRVDEYVAPVFFKGQAVGEVHLGVDRAAVDRVLG
                     HMRADLVEMILLFSVVIIGGLYLLSLIVIRPFRKILVGVNAVSSGDFNAKIELDTKDE
                     FGQLATIFNDMTIKLQESQKGLVEQERLQKEMQVAQEIQHTLLPASFPQIEGYEIGAT
                     YRAAKEVGGDYYDFFWVDPTTLGIVVADVSGKGVPGSMVMTMIRTAMRMEARGNKSAS
                     SVLAQVNGHVTGDMKKGMFVTMFYIILDSRNRSINFASAGHNPMILYRSSTREVFFLK
                     PKGFPVGIDLPEEDMFSRNMALQKIKLEKDDVLLIYTDGITEAMNIKKEQFGEKRLIQ
                     VIKDNYQLTPAELVQKLNEAIAQFTSGAEQNDDITVVAIKEKMKAETVQFKMRKRLLD
                     LVEKKGMSVAQACRQMHVSTKVYYKYKKLFDEQGVKGLKSVRADRSIEELSNDQKDAV
                     MALVEAHPDWSFSKIVEALRNDPENPHEVEARKVREFLKRKGIGGPALPGLPASKPAR
                     PRARRPKPAELSADERSAVERTLGQNAALGAREIVELLKKDPGAPLALDERSVRKYLK
                     GRMVEAAVAPIDAAHPPASGAAPNEVDTP"
     gene            4266..4628
                     /locus_tag="VFR02_01725"
     CDS             4266..4628
                     /locus_tag="VFR02_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9869204.1"
                     /translation="MDNISVNVSSHPKNKDISVLAVRGFIDTTTAPEFERTFHDVLDS
                     KKYKLIVDLKDVNYISSAGWGIFISELKRIRKQKGDLILVGMNPEVAEVFELLEFNTI
                     LKSFPNVDQAVIKGFQKS"
     gene            4747..>5734
                     /locus_tag="VFR02_01730"
     CDS             4747..>5734
                     /locus_tag="VFR02_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869205.1"
                     /translation="MSSLVYLGGRVISQREFDLADGLAGEGLKRLLEETHHVRAVELE
                     SIFRLSRKVDEKPTPDSCWKMGVIFMSNGFYEDAKQKFIKAIELDPSHLQSIKNYGIA
                     LTLTEDLEGAISILNRAQELGPSFADIYYHLGNVHLYRKELDGAVQCYLQALQINPRY
                     ADARLRLATACVGYLANGSVTAEDKIVELSERAKTEAETAATLNPKVRNREFMVSMDM
                     IKNRSFPVAFQHLLDARPRYLPRIGDEIIFFFTLTLLYGSEGIDLQMTEQYIDKLSKV
                     IEEYPHYADLHHHLGVAYLIKCRFDVNRSLKEFKKALEINPQFQKALNNSRDT"
BASE COUNT         1170 a         1801 c         1802 g          961 t
ORIGIN      
        1 gtttggcgcc cctgggggcc caggagaccg gcgggacggt atcgcccttc gacctggggg
       61 ggtcggcccg caccctgggg atgggcgggg tcggcgtggc cctgtcgggc gacggggcct
      121 ccttcctgcg caatccggcc gtcctggcca ccctgggcga gcaggagctg ttgaccttcc
      181 attccccctt gctcgcggac gcctcctacg acgcgttggg gttcgtgttg cccgtggctt
      241 cccacgccag tttcggcgcc gcggtggagc gggtgtccgt cacgggcatc caggagacca
      301 ccgacagcgt cagccccacg gggaccctgg cggacgagca attgcagggc gtgctgggtt
      361 acggcctgga gatctgggac gggctcagcc tgggtgggaa cgtcaagtac gaccggcaga
      421 ccctgggtag ctaccaggac ggcggcatgg gcgccgacgt ggggctgttg taccggctcg
      481 gccgttccgc caaggattat gaggggttcg gcctggcgaa catgacgctc ggcttttccg
      541 tgtccaacct ggtggccccc gagagccacc tgttccaatt ggccgatcag ccggcccggg
      601 tctaccaacc ggccctctcc taccggtacg agtccgccga ccaatccaac gccttgtggt
      661 tggcggtgga agggcgggtg gtggagaacg gcgcttccca ggtgctggcg ggcctggaat
      721 acggctggaa ccagacgatc ttcggccggg tgggttatga cggggtcggc cccaccgccg
      781 gggcggggct gcggttcgag ggcctggaat tcgattacgc cttcaaccag cgggacctgg
      841 gcgccctgca gcgtttttcc ctgacctaca gcttcaaccc ctaccgcgac ccccaggcgg
      901 ggcagaaaat gaagaccatg aaattcatcg cccagagcta cgaccgcgag aacgagtatg
      961 acgccgccat ccaggcctgg aagagcgtgg ccgacgagtt cccggacgac ggggatcccc
     1021 cggccgccat ccgttccctg caggaccggc gccaaaaagc cgttcgatcc gacctggacg
     1081 agggccggca ggccctggag gcgaacaacc tggaccgggc catccggttc ctggcccacg
     1141 gcctgagcct ggaccccacc aacccgcaga tcaagaagat cctgggccgg gtcaaccatg
     1201 aaatggtcct gacctccaac tacatgcgcg gcaccgaggc ctacagccgg gaggactaca
     1261 agaccgccgt gcggtacctg ggagcggtgt atgacctgga cccgggatac cgggacacgg
     1321 ccttcctgta ccatgacgcc cagagccact acatgcccct ggaaagcatg gaccgggagg
     1381 cgggggacca ctatgccctg ggcgtgaacg cctacatggc gggccaatac gcgcaggcca
     1441 tcgatgagtg gaagaaggtg ttggagaagg aacccaagaa ctacctggtg cgccgcaata
     1501 tcgaggaggc ccggcagttg ctgcaagccc agggcgatgc cggcgccgcc aagccgccca
     1561 aggactgacc atggacctca agaaattcat ccccgacctc accgaactca tcccgttcct
     1621 ccggccccat cggcgcgtgt tccgcctgga agtgccggcc aaagagattg agctcttgac
     1681 catcaaggac ttcatccagc gcgtctgcga cgccgcgggc tgctcttcca aggaaacttc
     1741 cagcgtgaag ctggccatcg acgaggcctg cagcaacatc gtgcgccacg cctaccaggg
     1801 ccgggaggac ggcaagatca tcctggaggc gggcatcggg gtgaaggaca tccgcatcgc
     1861 catcacggat ttcggcaagt ccttcgactt ccgcaagatc gaggcgcccg acctgaacca
     1921 ctacgtggac atcggcaaga agggcggcct ggggatcttc ctcatcaaga agatcatgta
     1981 caaggtggat taccgcacga aggacggccg caacgacctc atcctgcacc gcaggctgtc
     2041 ccaggcgccc ccggccgccg ccctggccaa gggccagcgc acgctttcga tctcctcccg
     2101 gttcacgatc atgtccacgg gcctgatctt cctgctgatc gtgctcctgt atttcttcct
     2161 gaacttccgg aaccagaact tcatcctgag ccaacaacgg gacaaggccc tctccttgac
     2221 ccaggccatg gccgcccagg ccggggaatc ggtcgtgcgc aaggacgacc tgggattgga
     2281 ccacctcatc gggcaggtcg tgcgggccga cgggggacgc gtgttcgatt acgtcttcgt
     2341 ggtcaaccag gacgacaaat tcctggccca ttcggacctg aaacaggtct tccaggaata
     2401 ccaccggccc cgcacggtca agccggtccg ccagggcggg gtgcgggtct tcgaggtcaa
     2461 gaccccccag gggcgggtgg acgaatacgt ggcgccggtc ttcttcaagg ggcaggcggt
     2521 gggggaggtg cacctggggg tggatcgggc ggcggtggac cgggtgctgg gccacatgcg
     2581 ggccgacctg gtcgagatga tccttctttt ctcggtggtc atcatcggcg ggctttacct
     2641 gctgtccctc atcgtgatcc gtcccttccg caagatcctg gtgggggtca acgcggtcag
     2701 ttcgggcgat ttcaacgcca agatcgagct ggacaccaag gatgagttcg gccaattggc
     2761 cacgatcttc aacgacatga cgatcaagct gcaggagtct cagaaaggcc tggtggaaca
     2821 ggaacgcctg cagaaggaga tgcaggtggc ccaggagatc cagcataccc ttttgccggc
     2881 ttctttcccc cagatcgagg gttatgagat cggggccacc taccgggccg ccaaggaagt
     2941 gggcggcgac tattacgact ttttctgggt cgaccccacc accctgggca tcgtggtggc
     3001 cgacgtgtcg ggcaagggcg tgcccgggtc gatggtcatg accatgatcc ggaccgccat
     3061 gcgcatggaa gcccggggca acaagtccgc ctccagcgtg ttggcccagg tcaacgggca
     3121 cgtgaccggc gacatgaaaa agggcatgtt cgtcaccatg ttctacatca tcctcgattc
     3181 gcgcaaccgc agcatcaatt tcgccagcgc ggggcacaac cccatgatcc tgtaccgcag
     3241 ttccacccgg gaggtgttct tcctgaaacc caaggggttc cccgtgggca tcgacctgcc
     3301 cgaggaggac atgttctcgc gcaacatggc gctccaaaag atcaagctgg agaaggacga
     3361 cgtgctgctc atctatacgg acggcatcac ggaagccatg aacatcaaga aggaacagtt
     3421 cggcgagaag cggctcatcc aggtcattaa ggacaactac cagttgaccc ccgccgagct
     3481 ggtgcagaaa ctcaacgagg ccatcgccca attcaccagc ggcgccgagc agaacgacga
     3541 catcacggtg gtggccatca aggaaaagat gaaggctgaa acggtccagt tcaagatgcg
     3601 caaacggctc ctggacctgg tcgagaaaaa ggggatgtcg gtggcccagg cctgccgcca
     3661 gatgcacgtt tccaccaagg tctactacaa gtacaagaag cttttcgacg agcagggcgt
     3721 gaagggcctc aagtccgtcc gggccgaccg gtccattgag gaactttcca acgaccaaaa
     3781 ggacgccgtg atggccctgg tggaggccca tcccgactgg agtttttcca agatcgtgga
     3841 ggcgctgcgc aacgatccgg agaaccccca cgaggtcgag gcgcgcaagg tccgcgagtt
     3901 cttgaagcgc aaggggatcg gcgggccggc gctgccgggc ctgccggcct ccaagccggc
     3961 gcggccccgc gcccgtcggc cgaagccggc ggagctttcg gccgacgaaa ggtcggcggt
     4021 ggaacggacg ctgggacaga acgccgccct cggggcccgg gagatcgtgg aactgctcaa
     4081 gaaagatccc ggggccccct tggccctcga tgaaaggtcg gtccgcaagt acttgaaggg
     4141 aaggatggtg gaagcggccg tcgcgccgat cgacgcggcg catccgccgg cgtccggcgc
     4201 ggcgccgaac gaggttgaca ccccctgaag tagccgtgat aatagctcat ctttttggag
     4261 gatttatgga taacatctcg gtcaacgtct cgtcccaccc caaaaataag gacatcagcg
     4321 tgttggccgt ccgcgggttc atcgatacca ccaccgcccc ggaattcgag cgcaccttcc
     4381 atgacgtcct ggacagcaag aagtacaagt tgatcgtgga cctgaaggac gtcaattaca
     4441 tcagcagcgc gggatggggc atcttcatca gcgaattgaa gcgcatccgc aaacagaagg
     4501 gggacctgat cttggtgggc atgaaccccg aagtcgccga ggtcttcgag ctcctggaat
     4561 tcaacaccat cctcaagtcg ttccccaacg tggatcaggc cgtcatcaag ggtttccaga
     4621 agtcttgaag ggccgacgga aacccacgac tggataaaaa tggaaccggg cgctttgagt
     4681 tccctcattg aaattgaggg caaaaatttc acgattgaga cgtcggcttc gccccaaaag
     4741 aaccgggtgt cgtctctggt ctacctgggg ggccgcgtga tctcccagcg ggaattcgac
     4801 ctggccgacg gcctggccgg ggaaggcctc aagcgcctgt tggaggagac ccaccatgtg
     4861 cgggccgtgg aactggaatc gatcttccgg ctttcccgca aggtcgacga gaagcccacc
     4921 cccgattcgt gctggaaaat gggcgtgatc ttcatgtcca acggtttcta cgaggacgcc
     4981 aaacagaagt tcatcaaggc catcgagctg gacccctccc acctgcagtc gatcaagaac
     5041 tacggcatcg ccttgaccct gaccgaggac ctggaagggg cgatctccat cctgaaccgg
     5101 gcccaggaac tggggccttc cttcgcggac atctattacc acctggggaa cgtgcacctt
     5161 taccgcaagg aactggacgg ggcggtgcag tgctacctgc aggcgctcca gatcaatccc
     5221 cgctacgccg acgcccgctt gaggctggcc acggcctgcg tgggctattt ggccaacggc
     5281 agcgtgacgg cggaggacaa gatcgtggaa ctttccgagc gggccaagac cgaggcggag
     5341 accgccgcca cgctcaaccc caaggtccgc aaccgggagt tcatggtgtc catggacatg
     5401 atcaagaacc gcagcttccc ggtggccttc cagcacctgc tggacgcgcg cccgcgctac
     5461 ctgccgcgca tcggggacga gatcatcttt ttcttcaccc tgaccctgct ctacggcagc
     5521 gaggggatcg acctgcagat gaccgagcag tacatcgaca agctgagcaa ggtgatcgag
     5581 gagtacccgc actacgcgga cctgcaccac cacctgggcg tggcctacct catcaaatgc
     5641 cgcttcgacg tgaaccgttc cctgaaggaa ttcaagaagg ccctggagat caacccccag
     5701 ttccaaaagg ccttgaacaa ttccagggac acgg
//
LOCUS       DASTJJ010000142         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_755776, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000142 DASTJJ010000000
VERSION     DASTJJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755776"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFR02_01735"
     CDS             <1..277
                     /locus_tag="VFR02_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET9869206.1"
                     /translation="FDLVFIDADKPGYPAYLAWAMDHLRVGGAVLGDNTFSGGLAADA
                     ANETKGPKAATVKAIRAFNRALAEDPRFRATILPTGEGLTFGVKVKP"
     gene            274..1008
                     /locus_tag="VFR02_01740"
     CDS             274..1008
                     /locus_tag="VFR02_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869207.1"
                     /translation="MRARWTAIALLLAAVLQGRAQPVPTPTPTAGTPPDAREAAALLE
                     QGRSNLQEAPLERALLYYSSRPGDPDAVRACARIDYYRALGEDSRKQRDRAKKILDEG
                     IGWAQQAVRSDPGSGPAHALLADLYGEKIALGDVFTAMDIGPKDAQENEKALSLAPLD
                     AGVLAAQGRRYLMAPDFAGGDPRKALVFFRRSLEADPDSDETWLWLARACRRLKDPAG
                     FQQALDKARSLDPQNVQVEWEAEKKP"
     gene            1162..>2225
                     /locus_tag="VFR02_01745"
     CDS             1162..>2225
                     /locus_tag="VFR02_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869208.1"
                     /translation="MKRAFRSFSRREWVLGGACLVWLGWVFWRHSTLPSLGDLAFLGE
                     LRAGLSEAHGAQAFANLTAALPAWSWALGALVTMAALGKRLSGWLGAPPMNRALGLAQ
                     AFGLGMIALTTLWLGLGLNGLWERPVFVGSALGGLAWGVRSLTRRSPVRPPRMPFWAW
                     GPALLAGLLLLLALAQSLVPEAYFDGLVYHLSVPRFWLDRHGLLDDPTCFHSYYPFGA
                     EIYFLNGFWSGDPAGAKLLNTASLVLAALAAGGWAAEAAGPAWGFLTGASLFFVPWVS
                     TTVWTTQNEVVLGLFLLLCSYCAKRWAEADPAARRPWAWGMGLMGGAAWTVKYTALPA
                     FVALAAALWLRDRRVFSRER"
BASE COUNT          292 a          739 c          790 g          404 t
ORIGIN      
        1 cttcgacctg gtcttcatcg acgccgacaa gccgggctac ccggcctacc tggcctgggc
       61 catggaccat ctgcgggtgg gcggggccgt cttgggcgac aacaccttct cgggcggctt
      121 ggcggcggac gcggccaacg agacgaaggg ccccaaggcc gccacggtca aggccatccg
      181 ggccttcaac cgcgccctgg cggaggaccc gcggttccgc gccaccatcc ttcccacggg
      241 ggaaggcctg accttcggcg tgaaggtgaa gccgtgaggg cccgatggac ggccatcgcg
      301 ctcttgctgg cggcggtcct tcaaggccga gcccaaccgg tgcccacgcc caccccgacg
      361 gccgggacgc cgcccgatgc ccgggaagcc gccgccctct tggaacaggg ccggtccaac
      421 ctgcaggaag cgcctttgga acgggccctt ctttattatt cgtcccggcc cggggacccg
      481 gacgcggtcc gggcctgcgc gcgcatcgac tattaccggg ccttgggcga ggattcccga
      541 aaacaacggg accgggccaa gaagatattg gacgagggga tcggctgggc ccaacaggcg
      601 gtccgatcgg acccggggtc ggggccggcc cacgccctct tggcggacct ttacggggaa
      661 aagatcgcct tgggggatgt cttcaccgcg atggatatcg ggcccaagga cgcccaggag
      721 aatgagaagg ccctgtccct ggcgcccctg gacgccgggg tgttggccgc ccagggacgc
      781 cgctatctca tggcccccga tttcgccggg ggcgaccccc ggaaggcgtt ggtctttttc
      841 cggcggtcgc tggaggcgga cccggattcg gacgagactt ggctttggtt ggcgcgcgcc
      901 tgccgccgct tgaaggaccc ggcggggttc cagcaggcct tggacaaggc ccggagcctg
      961 gacccgcaga acgtccaggt ggaatgggaa gccgaaaaaa agccctgaga caagctcccg
     1021 ccttcgcggc ccgcctttat aatgaaagcc gctccaaaga cgcgccctgg aaggatccat
     1081 gcggaaaaac cctttcatct tagtcccgga ctcccaggcc ttcccgggtg ggccggcctt
     1141 ccggccggtc cggggccgtc gatgaagcgg gctttccggt ccttctcccg tcgggaatgg
     1201 gtcctgggcg gggcctgcct ggtgtggctg ggttgggtct tttggcgcca ttccacgctt
     1261 ccaagcttgg gggacctggc tttcctgggg gagctgcgcg cggggctttc ggaggctcat
     1321 ggggcccagg ccttcgccaa cctgacggcg gccctgcccg catggtcctg ggccctgggc
     1381 gccctggtga ccatggcggc cttggggaaa aggctcagcg gttggttggg agcgcccccc
     1441 atgaaccggg ccttggggct ggcccaggcc ttcgggttgg gaatgatcgc cctgaccacc
     1501 ctttggctgg gcctgggctt gaacggcctc tgggaaaggc cggtcttcgt cggatccgct
     1561 ttagggggac tggcctgggg cgtccggtcc ctgacgcgcc gaagtccggt ccgcccgccc
     1621 cggatgccct tttgggcttg gggcccggcc cttttggcgg ggctcctact gctgttggcc
     1681 ttggcccaaa gcctggtgcc cgaggcctat ttcgacggcc tggtctacca cctgtcggtg
     1741 ccgcgctttt ggctggaccg tcacggcctg ctggacgatc ccacctgttt ccattcctat
     1801 tatcccttcg gcgccgagat ctatttcctc aacggttttt ggagcggcga tcccgcgggc
     1861 gccaagctgc tcaatacggc ctccctggtc ctggccgcct tggcggcggg aggctgggcg
     1921 gcggaggcgg cggggcccgc ctgggggttc ttgacggggg catcgctctt tttcgtgccc
     1981 tgggtctcca ccacggtctg gaccacccag aacgaggtgg tgctggggct tttcctgctc
     2041 ctttgttctt attgcgccaa gcgctgggcc gaagccgacc cggcggcccg gcggccctgg
     2101 gcctggggga tgggcttgat gggcggcgcg gcttggacgg tcaagtacac ggccctgccg
     2161 gccttcgtgg cgctggcggc ggcgctgtgg ctgcgggacc ggcgcgtctt ttcgcgcgag
     2221 cggtg
//
LOCUS       DASTJJ010000143         1492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_266983, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000143 DASTJJ010000000
VERSION     DASTJJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266983"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1492
                     /locus_tag="VFR02_01750"
     CDS             <1..>1492
                     /locus_tag="VFR02_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9869209.1"
                     /translation="VGDIGEGLVVLEGTRVVYANETFQKMTGYSEEQLLNISSFFNLL
                     EGSDKARQQEKIAAFRKNPRKPLRFESPQRNDAGKPMTAEITLTVLKDDPQDRMVGLV
                     RDVTDQKRLMEAVKDQEVQYKLLFKNNPSPMFIYDLENLAILSVNDAAILKYGFTEKE
                     FLSMSLRDLRRAEDIPQMLEQLEGLKRGGNVLKEEVLHRKKDGSLIEVEVISHSTLFE
                     GRSARVMLAYDLTEHRKAENALRESERRLSAVVTNAPIILFTLDMDGKVALSEGSGLK
                     SLRLKPGGLVGKSAFDLYKDNPAMISLLRRAIGGESVSGTVEIGGLFFETRCSPLAGD
                     DGKPQGVLGMAIDVTETKRAEQALVDSERKFRELFNNANDAIFLYPLSDEAVPGKFLE
                     VNDIACQKLGYSRSELLHMTPLDVVAPEGLGEMPKIYKRSLKQEHITYEKVHVTKSGM
                     KIPVEVNAHTFLRNGQKAVLSIARDVTDRKRAEETIRRQAYYDVLTN"
BASE COUNT          314 a          487 c          467 g          224 t
ORIGIN      
        1 ggtcggggac atcggggagg gcctggtggt gctggaaggc acccgcgtgg tctacgccaa
       61 cgagaccttc cagaagatga ccggttattc ggaggagcag ctcctcaaca tctcctcctt
      121 cttcaacctg ctggaggggt cggacaaggc ccgccagcag gaaaagatcg ccgctttccg
      181 caagaacccc cgcaaacccc tccgtttcga gtccccccag cgcaacgacg ccggaaaacc
      241 catgacggct gagatcaccc tgaccgtgct caaggacgac ccccaagacc gcatggtggg
      301 cctggtgcgg gacgtcaccg accaaaaacg cctgatggaa gccgtcaagg accaggaagt
      361 ccagtacaag ctgctcttca agaacaaccc cagccccatg ttcatctacg atctggagaa
      421 cctggccatc ctgtcggtga acgacgcggc catcctcaag tacggcttca ccgagaagga
      481 attcctgtcc atgtccctgc gggacctgcg gcgggcggag gacatccccc aaatgctcga
      541 gcagttggag gggctgaagc ggggcgggaa cgtcctgaag gaggaggtcc tgcaccgcaa
      601 gaaggacggc tccctgatcg aggtggaggt catctcgcat tccacgctct tcgagggccg
      661 ctcggcccgg gtgatgctgg cctacgacct gaccgagcac cgcaaggccg agaacgccct
      721 aagggagagc gaacggcgcc tgagcgcggt ggtcaccaac gcgcccatca tcctcttcac
      781 cctggacatg gacggcaagg tggccctttc ggaagggtcg ggcctgaagt ccctgcggct
      841 gaaaccgggc gggttggtgg ggaagtccgc cttcgacctc tacaaggaca acccggccat
      901 gatctcgctg ttgcgccggg ccatcggcgg ggaatcggtg agcggcacgg tggagatcgg
      961 cgggctcttt ttcgagaccc ggtgttcccc cctggcgggg gacgacggaa agccccaggg
     1021 cgtgctgggc atggccatcg acgtcaccga gaccaagcgg gccgaacagg ccctggtcga
     1081 cagcgaacgc aagttccgcg agctcttcaa caacgccaac gacgcgatct tcctctatcc
     1141 gctgtccgac gaagccgtgc ccgggaagtt cctggaggtc aacgacatcg cctgccagaa
     1201 gctgggctac agccgcagcg agctgctcca catgaccccc ctggacgtgg tggcgcccga
     1261 gggcctgggc gagatgccca agatctacaa gcggtccctc aagcaggaac acatcaccta
     1321 cgagaaggtc cacgtgacca agtcgggcat gaagatcccg gtggaggtca acgcccacac
     1381 tttcctgcgc aacggccaga aggcggtgct gtccatcgcc cgggacgtga ccgaccgcaa
     1441 acgcgccgag gagaccatcc gccggcaggc ctattacgac gtgctcacca ac
//
LOCUS       DASTJJ010000144         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1244682, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000144 DASTJJ010000000
VERSION     DASTJJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244682"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..590
                     /locus_tag="VFR02_01755"
     CDS             51..590
                     /locus_tag="VFR02_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9869210.1"
                     /translation="MTDFRQAGDFQAFVTAHRGLWFKLAFRVLEDREEAEDVVQETLA
                     FLWEKRQVLSIGHPGAYVARAVWLNSIKRRTRRRLALSLDEVPEPAAGEAQEPEEWDP
                     RHLEEALGALPPAQQTVVRMKYYMGLTFKEIGETLKISLHTAASRCRYALAAMGKDLE
                     GRANPAGKPKGPRPTGGKG"
     gene            587..976
                     /locus_tag="VFR02_01760"
     CDS             587..976
                     /locus_tag="VFR02_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869211.1"
                     /translation="MSEETKETGKAHASVVFGGGSAIAVAGILGGLLLAVGFPWVVLV
                     YGQGLGKVLVVVFLGLGLLLGTTVSLASAVLGLVMPRQVGGGAPQGLLRELKDWSREW
                     RQWAEVDWKHWSEEEWKEWAERQKRGE"
     gene            1106..2542
                     /locus_tag="VFR02_01765"
     CDS             1106..2542
                     /locus_tag="VFR02_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9869212.1"
                     /translation="MRRLSFALGLFLAAPALSAQTLSLSDYLGQVKGQGPDYQAQAAA
                     VEGYTKQSHQQDLTYSPVLTAGYNHRNDQSLQSSLLAPDQTLSDSAGVSITDILPFGP
                     KLTLGYAFNNINISYSPQLISSFGPFASDFLTSYYQVSPVVSLSLPLFKDFGGSQTQA
                     GVKKAQYQLESAADSAAFQRGQELYDAQVAYWNLALARQTLAIRQDTLDRTQKIWDWT
                     KKRVARNLADPPDALQAEASVRLAALDLDMAQQQERSARLAFNRYRGASTDAVTEQLE
                     DLQSSLADFPKTLPDQAPDRLDLKAVERTALQQKAAYDEAHQNVYPDLTANASWRGNG
                     LDPVFGTANHQAFAADHPTYDVGVSFNLPLDVFTAARVAEGYQKNYESAELALKDKKL
                     QVAQDWQDLKDKLGDVDQRLDMTAQIEAIQKDKADQEKQRLEYGRTTEFQLLSFENDY
                     NAARLSHLSVIAEKLSLLARAQWWMAAE"
     gene            2700..>3935
                     /locus_tag="VFR02_01770"
     CDS             2700..>3935
                     /locus_tag="VFR02_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9869213.1"
                     /translation="MSLPKLAVDRPVFVSCLVILIMVLGAMAYESLGVDLFPDVSFPY
                     VFVSTPYPGASPEEIESLISKPLEEELSSLQGVKKVSSTNQEGFSTVVVQFTLETDAK
                     DAEQQVRDRVAYVRPQLPKGIDEPVIRRMDPADQAVIQLSLRSTLSAADAFDLADQVV
                     KPDLAQVPGVGVVNIQGGTKREIHVLLDRDKLNAYRISASHVADRIGLNGENVPVGDV
                     NIGDKTLTFRSVGQYDDLNRLKQTSVNFTGSDVSVPVSRLGTVEDSTVKPTGYSYVNG
                     QPALILQVYKQSKANTVAVVDGVMARMDKINEDLKSRPGAATLGLVQEAALPVRLNLN
                     DVKHTILLGVSLTVCVVFLFLGNLRSTFITVTALPVSLLGAFILMKTMGFTLNVMTLL
                     ALSLAVGLLIDDAIVVRENI"
BASE COUNT          713 a         1300 c         1274 g          648 t
ORIGIN      
        1 aaaccagggg gggagggatc catccctcga gcgaggttaa aatcccagtc atgaccgact
       61 ttcgacaagc gggggacttc caggccttcg tcacggccca ccgggggctt tggttcaaat
      121 tggccttccg ggtgctggag gaccgggaag aggccgagga cgtggtccag gagaccctgg
      181 ccttcctttg ggaaaaacgc caggtgcttt ccatcggcca cccgggcgcc tacgtggcgc
      241 gggcggtctg gttgaactcc atcaagcggc gtacccggcg gcgcctggcc ctgtccctcg
      301 acgaggtgcc cgagccggcc gccggggaag cccaggagcc ggaggaatgg gatccccggc
      361 atctggagga ggccctgggg gccctgccgc cggcccaaca gacggtggtg cgcatgaagt
      421 attacatggg gctgacgttc aaggagatcg gggagaccct gaagatctcc ctgcatacgg
      481 cggccagccg ttgccgctac gccctggcgg ccatgggcaa ggatttggaa gggcgcgcaa
      541 acccggccgg gaagcccaag ggcccccggc ccacaggagg aaaaggatga gcgaggaaac
      601 gaaggaaacg ggcaaggcgc acgccagcgt ggtgttcggc gggggcagcg ccatcgcggt
      661 ggcgggcatc ctgggggggc tgctgctggc ggtcgggttc ccctgggtcg tgctggtgta
      721 tggccaggga ctgggcaagg tgctggtggt ggtcttcctc ggcctgggat tgctcttggg
      781 gaccacggtg tcgctggcct cggcggtctt gggactggtc atgccccgcc aggtgggggg
      841 cggcgcgccg cagggcctct tgcgcgaact caaggactgg agccgggaat ggcgccagtg
      901 ggccgaggtg gactggaagc attggtccga ggaagaatgg aaagaatggg ccgagcgtca
      961 aaagcggggc gaatagggca agggccaccc ttcttttccc agggtttggg ggagcggggc
     1021 cggttttcgg gggattgaca attggccgac cgctcggtag atttctgatt ccttggccgg
     1081 tgaaacaagt ctttgaggag cgcacatgcg acgcctgtct tttgccctgg gcttgttcct
     1141 ggccgcgccg gccctttcgg cccagaccct ttcgctctcc gattacctgg gccaggtgaa
     1201 gggccagggc ccggattacc aagcccaagc ggccgccgtg gaaggctaca cgaagcaatc
     1261 ccaccagcag gacctcacct attcgccggt cctgaccgcg ggctacaacc accgaaacga
     1321 ccaatcgctc caatcctcgt tgttggcgcc cgaccagacc ctgtcggatt cggcgggggt
     1381 ctcgatcacg gacatcctgc cctttggtcc gaagttgacc ttgggctacg ccttcaacaa
     1441 catcaacatc tcctattcgc cgcagttgat cagctctttc ggccccttcg cctccgattt
     1501 cctcacgtcc tattaccagg tcagtccggt ggtctcgctg tccctgccgc tcttcaagga
     1561 tttcggcggt tcccaaacgc aggccggcgt caagaaggcg caataccagt tggagtccgc
     1621 cgccgactcg gcggccttcc aacggggaca ggaactctac gacgcccagg tggcctactg
     1681 gaacctggcc ctggcccgcc agaccctggc catccgacag gacaccttgg accgcaccca
     1741 gaagatctgg gactggacca agaagcgggt cgcccgcaac ttggccgacc cgcccgacgc
     1801 cctgcaggcc gaggcctccg tgcggctggc ggccctggac ctggacatgg cccagcaaca
     1861 ggaacgctcg gcccgtctgg cgttcaaccg ctaccggggc gcttccaccg acgcggtgac
     1921 cgagcaattg gaggacctgc agtcctccct ggccgatttt cccaagacct tgccggacca
     1981 ggcgcccgat cggctcgacc tgaaagcggt ggaaaggacg gcgctccagc agaaagccgc
     2041 ctatgacgag gcccaccaga acgtctatcc ggacctgacc gccaacgcct cctggcgcgg
     2101 caacgggttg gacccggtct tcggcacggc caaccaccag gccttcgcgg cggaccaccc
     2161 gacctacgat gtgggggtga gcttcaacct gccgctcgac gtcttcacgg cggcccgggt
     2221 ggcggaaggc taccagaaga actatgagag cgccgaactg gccttgaagg acaaaaagct
     2281 ccaagtcgcg caggactggc aggacctgaa ggacaagttg ggagacgtgg accagcggct
     2341 cgacatgacg gcccagatcg aggccatcca aaaggacaag gccgaccagg aaaagcaacg
     2401 gctcgagtac ggccgcacca ccgagttcca gctcctgtcc ttcgagaacg actacaacgc
     2461 cgcgcgcctg agccacctgt cggtcatcgc cgagaagctg tcgctcttgg cccgggccca
     2521 atggtggatg gcggccgagt aagccaagga gaaaccgtga gccttcccaa acgcgccgtc
     2581 gaccggccct gggtggggac ggggccggat ccgggaagaa aaaagtcccg gcccgtcccg
     2641 agacgggcct cgcgttgtgg atcacccgaa acgtggccgt gtacttttaa ggagtcagcg
     2701 tgagccttcc taaactcgcc gtcgaccggc ccgtcttcgt ttcctgcctg gtcatcctca
     2761 tcatggtcct gggcgccatg gcctatgagt ccctgggcgt ggaccttttc cccgacgtga
     2821 gcttccccta cgtcttcgtc tccacgccct acccgggcgc ctcccccgag gagatcgagt
     2881 cgctcatctc caagcccctg gaggaggagc tctcgtcgct tcagggcgtc aagaaggtct
     2941 cttccaccaa ccaggaaggc ttctccaccg tggtggtgca gttcaccctg gagaccgacg
     3001 ccaaggacgc cgagcagcag gtgcgcgacc gggtggccta cgtgcggccc caattgccca
     3061 agggcatcga cgagcccgtc atccgccgca tggacccggc ggaccaggcc gtcatccagt
     3121 tgagcctgcg atcgaccctc tcggcggcgg acgccttcga cctggccgac caggtggtca
     3181 agcccgacct ggcccaggtg cccggcgtgg gcgtggtgaa catccagggc ggcaccaagc
     3241 gcgagatcca cgtgctgctg gaccgcgaca agctcaacgc ctaccgcatc agcgcctccc
     3301 acgtggccga ccgcatcggc ttgaacggcg agaacgtgcc cgtgggcgac gtgaacatcg
     3361 gcgacaagac cctcaccttc cgctccgtgg gccagtacga cgacctgaac cgcctcaagc
     3421 agacctcggt gaacttcacc gggtccgacg tgtcggtccc cgtgtcccgg ctgggcacgg
     3481 tggaggattc cacggtcaag cccacgggct atagctacgt caacggccag cccgcgctca
     3541 tcctgcaggt ctacaagcag tccaaggcca acacggtggc cgtggtggac ggcgtcatgg
     3601 cgcgcatgga caagatcaac gaggacctca agagccgccc cggggcggcc acgctcggcc
     3661 tggtgcagga ggcggccctg ccggtcaggc tcaacctcaa cgacgtgaag cacaccatcc
     3721 tgctgggggt ctcgctgacc gtctgcgtgg tcttcctctt cctgggcaac ctcaggtcca
     3781 ccttcatcac cgtgacggcc ctgcccgtgt cgctcttggg ggccttcatc ctcatgaaga
     3841 ccatgggctt caccctgaac gtgatgaccc tgttggcgct ctccctggcg gtggggctct
     3901 tgatcgacga cgccatcgtg gtgcgcgaga acatc
//
LOCUS       DASTJJ010000145         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_512462, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000145 DASTJJ010000000
VERSION     DASTJJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512462"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFR02_01775"
     CDS             <1..192
                     /locus_tag="VFR02_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9869214.1"
                     /translation="DLLIINKTDLAPHVGADLSVMERDAARMRGKGRTIFAQVTKGVA
                     VGEIADFVLEAWQKALASA"
     gene            201..626
                     /gene="nikR"
                     /locus_tag="VFR02_01780"
     CDS             201..626
                     /gene="nikR"
                     /locus_tag="VFR02_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HET9869215.1"
                     /translation="MDALGPLRRFSFSIEAPLAERLQRMVRESRYANRSEFIRDLVRG
                     RMVEQLWERDAEALGTLSLLYDHHRRGLNERLVDIQHHQEARVLATTHVHLDGRLCAE
                     MIMARGRAGEIRHLFDHLRREKGVLHAALSLGSAGERLA"
     gene            837..1904
                     /locus_tag="VFR02_01785"
     CDS             837..1904
                     /locus_tag="VFR02_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HET9869216.1"
                     /translation="MGIAWWDDGPQRVGAKAGALYLLLVGANLAAWGFAFAAFGHRPA
                     LLGTAFLAYLFGLRHAVDADHIAAIDNVVRRLLQSGRRPITAGFFFSLGHSTVVVLAS
                     ALLAGAVGLSRDRWEAFHSLGATVGTAVSAFFLVGIGLANLLVLRGLWRVFRRMGRGE
                     TVREEEWEGLLSGRGFLARLLGPFLRGVTHSGQMYPVGFLFGLGFDTATEVGLLGLAA
                     AQAAQGLSFGDTLVFPALFTAGMDLVDATDSVLMTRAYQWAFVRPLRKLWYNLTLTAL
                     SVAVALVVGGLEALGLLAGQGGSQGGGWDLVRSLNSDLADFGFLVAGVFLAGWALSVF
                     VYRLQGYERAGPGPDAGVVLK"
     gene            1922..>2854
                     /locus_tag="VFR02_01790"
     CDS             1922..>2854
                     /locus_tag="VFR02_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9869217.1"
                     /translation="MPTLKTPRGWTLREWREAYASGGLTPRQALGALRRSLHPSDPAW
                     ISLADEAQWGSQLAGLEARLQAAEGDREALPLYGIPFAVKDNIDAAGWVTTAACPAFA
                     YTPAQDAPVVAALLAAGAVLVGKTNLDQFATGLVGLRSPYGAVPNAFDARYVAGGSSA
                     GSASAVARGLVPFSLGTDTAGSGRVPAAFHNLVGLKPTRGAVSTRGVVPTCRSLDCVS
                     ILALTLEDAEEVARLIGGYDEQDAYSRAAPPAALTRRSRPRRRFGVPQDPQWFGDERQ
                     ARAYARALERVRELNVELQPVDFQPMYEMSDLLYG"
BASE COUNT          402 a          937 c         1026 g          489 t
ORIGIN      
        1 gacctgctca tcatcaacaa gaccgacctg gcgccccacg tgggcgcgga cctgtcggtc
       61 atggaacgcg acgcggcccg catgcggggc aagggcagga ccatcttcgc ccaggtcacc
      121 aagggcgtgg cggtggggga gatcgcggac ttcgtgctgg aggcctggca aaaagccctg
      181 gcctccgcct gaggcccgcc atggacgccc tgggcccctt aaggcgcttc agcttctcca
      241 tcgaggcgcc gctggccgag cgcctgcagc gcatggtgcg ggaaagccgc tacgccaacc
      301 ggtccgagtt catccgcgac ctggtgcggg gccggatggt ggaacagttg tgggaaaggg
      361 acgccgaggc cctgggcacg ctctcgctgc tctacgacca ccaccgccgg gggctcaatg
      421 agaggctggt ggacatccag caccaccagg aagcccgggt gttggccacc acccacgtgc
      481 acctggacgg gaggctttgc gccgagatga tcatggcccg gggccgggcg ggggagatcc
      541 gccacctctt cgaccatttg cggcgggaaa agggcgtgct gcacgcggcg ctctcgttgg
      601 gcagcgcggg cgaaaggctg gcctgaggct tttgggcggg ccgggtatta cggaagctaa
      661 aaaaatatta cggaactacg ctttttacaa agcaagccaa agagaacttg aaacaatgaa
      721 aaacggatga aatctgatgt ttgaaaataa aaatcaagtt ttatcagctt ttatcaatgt
      781 tccaattgtc cttgctttgc gaagtgcatg attctgaatt attacggagg acggccatgg
      841 ggatcgcttg gtgggatgac gggccccagc gggtgggggc caaggcgggc gcgctttacc
      901 tgctgctggt gggcgccaac ctggcggcct ggggattcgc cttcgcggcc ttcgggcacc
      961 ggccggccct cttgggcacg gccttcctgg cctatctctt cggcctgcgc cacgcggtgg
     1021 acgccgacca tatcgccgcc atcgacaacg tggtgcgccg cctcctgcag tcgggccggc
     1081 ggcccatcac ggcggggttc ttcttttcgc tgggccattc caccgtggtg gtgctggcct
     1141 cggcgctctt ggcgggggcg gtgggccttt cccgggaccg gtgggaagcc ttccattccc
     1201 tgggcgccac cgtgggcacg gcggtctcgg ccttcttcct ggtgggaatc ggcctggcca
     1261 acctgctggt cctgcggggc ctatggcgcg tcttccggcg gatggggcgg ggggagacgg
     1321 tgcgcgagga ggagtgggaa gggctgctgt cgggccgggg attcctcgcc cggcttttgg
     1381 gccctttcct gaggggcgtc acccacagcg gccaaatgta tccggtgggg ttcctcttcg
     1441 ggttggggtt cgacacggcc accgaggtgg ggctcttggg cctcgcggcg gcccaggccg
     1501 cccagggcct ttcctttggg gacaccctgg tgttccccgc gctcttcacg gcgggcatgg
     1561 acctggtgga cgcgaccgac agcgtgctca tgacccgggc ctaccaatgg gccttcgtgc
     1621 ggcccttgcg gaaactttgg tacaacctca ccctcaccgc cctctcggtg gcggtggccc
     1681 tggtggtggg ggggctggag gcgctgggcc ttttggccgg ccaaggcggt tcccagggcg
     1741 gcggctggga cctggtgcgg tccctcaaca gcgacctggc cgatttcggg ttcctggtgg
     1801 ccggggtctt cctggcggga tgggccttgt cggtcttcgt ctaccggctt cagggatacg
     1861 aaagggcggg cccggggccc gacgccggcg tggtcttaaa ataaggaaag ggaccgaccg
     1921 gatgccgacc ctgaagacgc cccggggatg gaccttgcgg gaatggcggg aggcctacgc
     1981 ctccggcggc ctcacgcccc gacaggccct gggggccctg cgccgttcgc tccatccttc
     2041 cgacccggcc tggatcagcc tggccgacga ggcccagtgg ggatcccaat tggccggcct
     2101 ggaggcgcgg ctgcaggcgg cggagggcga ccgggaggcc ctgccgcttt acggcatccc
     2161 cttcgcggtc aaggacaaca tcgacgccgc cggttgggtg accacggcgg cctgcccggc
     2221 cttcgcctac acgcccgccc aggacgcgcc cgtggtggcc gcgctcttgg ccgccggcgc
     2281 cgtgctggtg ggaaagacca acctggacca attcgccacc ggcctggtgg gcctgcgctc
     2341 gccctatggc gccgtgccca acgccttcga cgcgcggtac gtggccggcg gttccagcgc
     2401 ggggtcggcg tcggcggtgg cccggggcct ggtgcccttc agcctgggca cggacacggc
     2461 gggctcgggc cgggtgcccg cggccttcca caacctggtg ggcctcaagc ccacccgggg
     2521 cgcggtcagc acccggggcg tggtgcccac ctgccggtcc ctggactgtg tgtccatcct
     2581 ggccctcacc ctggaggacg ccgaggaggt ggcccggctc atcgggggtt acgacgagca
     2641 ggacgcctat tcgcgcgcgg cgccgcccgc ggcgctcacc cggcggtccc ggccccggcg
     2701 ccgtttcggc gtgccgcagg atccccagtg gttcggcgac gaacgccagg cccgggccta
     2761 tgcccgggcg ctggagcgcg tgcgggaact caacgtcgag ctccaacccg tcgatttcca
     2821 gcccatgtac gagatgtcgg acctgctcta cggc
//
LOCUS       DASTJJ010000146         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1735322, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000146 DASTJJ010000000
VERSION     DASTJJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735322"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFR02_01795"
     CDS             complement(<1..1086)
                     /locus_tag="VFR02_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET9869218.1"
                     /translation="MQDATLWRTLLGVEKTIVEDVNYDEETGILIASVRPTASQLRRC
                     GECRRRCPKYDQGEGRRRWRGLDIGTVPVLLEADAPRVKCRKHGVSVAAVPWARHQAG
                     HTKVFDQQVAWLATQTSKTAITQLMRIAWRTVGAIITRVWNDTAATFDPFANLTRIGI
                     DEISYKRGHKYLSVVVDHDTGRLVWASPGRDKATLRKFFDALGAERSAQITHVSADAA
                     DWIASVVAEKCPHAIRVADPFHVVKWATDALDEVRRATWNEARKDARTNEAKRGRGRP
                     PKDAPPRPDSERAKKLKNSRYALWKNPEDLTEKQQAKLAWIVQTDPRLGRAYYLKEGL
                     RTVFKLPYRDAADALDKWVAWARRSRIP"
     gene            complement(1207..>1408)
                     /locus_tag="VFR02_01800"
     CDS             complement(1207..>1408)
                     /locus_tag="VFR02_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9869219.1"
                     /translation="IEVWISAVKGASLTVAYAIFDPVTGMKCAIAETTIAFFHEGSGT
                     VLRIAAEKKAQLAPLLGEANFH"
BASE COUNT          207 a          437 c          477 g          287 t
ORIGIN      
        1 cgggatccgg gacctcctgg cccaggcgac ccatttgtcc agggcgtcgg cggcgtccct
       61 gtagggcagt ttgaagacgg tgcgcaggcc ttccttgagg tagtacgcgc ggccgagccg
      121 ggggtcggtt tgcacgatcc aggcgagctt ggcctgctgt ttctcggtga ggtcctctgg
      181 gttcttccag agcgcgtagc gggagttttt cagcttcttc gcccgctcgc tgtcgggccg
      241 gggcggggcg tccttgggcg ggcgtccccg gccccgcttg gcttcgttgg tccgggcgtc
      301 tttgcgggcc tcgttccagg tggcgcgccg gacctcgtcc agggcatcgg tggcccactt
      361 tacgacgtgg aaggggtcgg ccacgcggat ggcgtggggg catttctcgg ccacgacgga
      421 ggcgatccag tcggccgcgt cggcggaaac atgcgtgatt tgggcgctgc gttccgctcc
      481 gagcgcgtcg aagaatttcc tcagcgtggc tttgtcccgg cccggggagg cccacaccag
      541 ccggccggta tcgtggtcaa cgacgacaga aagatacttg tggccgcgct tgtaggagat
      601 ctcgtcgatg ccgatccgtg tcaggttcgc gaacgggtcg aaggtggcgg cggtgtcgtt
      661 ccacacgcgg gtgatgatgg cccccacggt ccgccaggcg atgcgcatca actgggtgat
      721 cgcggtcttg gaggtctggg tggccagcca ggcgacctgc tggtcaaaaa ccttggtgtg
      781 ccctgcctgg tggcgggccc acggaacggc ggccacgctt accccgtgct tgcggcactt
      841 cacccgtggg gcgtctgctt ccaggagtac cgggaccgtg ccgatgtcca gtccccgcca
      901 tcggcggcgt ccttcgccct ggtcgtattt tgggcaccgg cgccggcatt caccacagcg
      961 gcgcagctgg gaggccgtgg gcctgacgga ggcgatcagg atgcccgttt cctcgtcgta
     1021 gttgacgtcc tccacaatcg tcttctcgac accgagcagg gtccgccata gagttgcatc
     1081 ttgcacgccg ttttccgttc ttcaagtttt tttccttaga cagtcaaaaa cttaggcgga
     1141 aagcggcgtg tgctcgttaa ggcgcgccaa aaagacccac ggatacgtca ggagagcctg
     1201 aaaatttcag tgaaagttcg cctcgccgag aagcggggcc agctgcgcct tcttttccgc
     1261 cgcaatgcgc aggacggtcc cgctgccctc gtggaagaac gcgatggtgg tttccgcaat
     1321 ggcgcacttc atgccggtga ccggatcgaa aatggcgtag gccaccgtca ggctcgcccc
     1381 cttcacggcg ctgatccaca cctcgatc
//
LOCUS       DASTJJ010000147         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_879803, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000147 DASTJJ010000000
VERSION     DASTJJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879803"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFR02_01805"
     CDS             complement(<1..444)
                     /locus_tag="VFR02_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET9869220.1"
                     /translation="MRHRLPDRAWWILGARGVLGLLLGAACVVFLLFRPGARPDPYGL
                     AFLFSPFETLAAILVVLGAYAFLDGLFAFLLGIQTYGKKDHWWSLILEGMLSVWLGIW
                     TWLHPESVAVLVLLYWIGAWAFFTGVLEVFQSLRPEEPGARMPFLT"
     gene            complement(441..605)
                     /locus_tag="VFR02_01810"
     CDS             complement(441..605)
                     /locus_tag="VFR02_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869221.1"
                     /translation="MGKRHYSIKERRQAFHIEQGYEKKGMPVKRAWAIAWATVRKLGA
                     GRKHAGGGHP"
     gene            complement(626..>1113)
                     /locus_tag="VFR02_01815"
     CDS             complement(626..>1113)
                     /locus_tag="VFR02_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET9869222.1"
                     /translation="GLDNIVVMVPFCRTPREADRVLAAMARHGLRRGKKGLKVYVMCE
                     IPANVILQEEFAKRFDGFSIGSNDLTQLTLGVDRDSGILTKLFDERDPAVMRSIEEVI
                     RRCRKTRTKVGICGQAPSDHPEFAAFLVRCGIDSISLNPDSVAAALKSVAAQEARIRR
                     A"
BASE COUNT          220 a          378 c          333 g          182 t
ORIGIN      
        1 cgtcaggaag ggcatccggg caccgggttc ctccgggcgc aaggattgga aaacctccag
       61 gacccccgtg aaaaaagccc aggcaccgat ccaataaagg aggaccagca ccgcgacgct
      121 ttcgggatgg agccaggtcc agatacccag ccacacgctc aacatcccct ccaggatcag
      181 ggaccaccaa tggtcttttt tcccataggt ctggatgccc aacaagaagg cgaaaagtcc
      241 gtccaggaag gcataggcgc ccaagaccac taggatggcg gccaaggtct caaaaggcga
      301 aaagagaaag gccaaaccgt agggatcggg ccttgccccc gggcggaaca gcaggaagac
      361 cacgcaggcc gctcccaata acaggcccaa gaccccccgc gcgcccagga tccaccaggc
      421 ccggtcaggc agtcgatgcc tcatggatgg cctccccccg cgtgtttgcg gccggcaccc
      481 agcttacgca cggtcgccca ggcgatggcc caggcccgtt tgacgggcat tccctttttt
      541 tcgtatccct gctcgatatg gaaggcctgc cggcgttcct tgatgctgta atggcgcttt
      601 cccatgactt cctccttttt cccgctcaag ccctcctgat ccgggcctcc tgggccgcca
      661 cgctcttgag ggcggcggcc accgagtccg ggttcagcga gatggaatcg atcccacagc
      721 gcaccaggaa ggccgcgaat tccggatggt cgctgggggc ctgcccgcag atgccgacct
      781 tggtccgggt cttccggcag cgccggatga cctcctcgat ggaccgcatg accgccgggt
      841 cccgctcgtc gaaaagtttg gtcaggatgc ccgaatcccg gtccacgccc agggtcagct
      901 gggtgaggtc gttggacccg atggaaaaac cgtcgaagcg cttggcgaac tcctcctgca
      961 gaatcacgtt ggccgggatc tcgcacatga cgtagacctt caggcctttt ttgccccgcc
     1021 gcaggccgtg gcgggccatg gcggccagca cccggtccgc ctcccggggc gtccggcaga
     1081 agggcaccat gaccacgatg ttgtcgagcc cca
//
LOCUS       DASTJJ010000148         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1124763, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000148 DASTJJ010000000
VERSION     DASTJJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124763"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFR02_01820"
     CDS             complement(<1..475)
                     /locus_tag="VFR02_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869223.1"
                     /translation="MDPWKRAAPFGVPLALAALTALFYRQVLWGGKIFVFVDASRFFY
                     PLWKWGAEALRRGLLPLWNPDAQFGTPYLADPQMAFAYPPWWLCYGLMPPTDAFAWLI
                     VLHHFWAMLGFWLWARHERFGTPAALLGTLAFGFSLHVVCSSWTPVALLTLSWVPW"
     gene            complement(487..>1466)
                     /locus_tag="VFR02_01825"
     CDS             complement(487..>1466)
                     /locus_tag="VFR02_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869224.1"
                     /translation="DAQFAHNEPLEWLTAFGLVGGVLALALLVRLWPREKGNGRSTAL
                     TALGTASFFDFCLHTPSIVLQAAGLLAPVSRGRAPFSWAGGLLGLGLVLGLFGSAAWT
                     PSLVARSQALEAGHRLPEALEGLSTAARLDAWDARVEAAQAGFLERLYLATGDPVWES
                     RSDEAWAGVLRLEAADGAWAQREARRWTARFEEFPGPASAAAARDAWGRAEQARPLDA
                     FVYFGEGLFLEEAARKLEKPVPGLGPARAEADFKKAAELEPNYAAAWVNLGHCQRSDP
                     AGRAEARDSFRTALAVHDRWKDAPGLAPLERDMVSLPPPVVAALRRDLK"
BASE COUNT          286 a          528 c          483 g          169 t
ORIGIN      
        1 tccagggcac ccaactcaag gtcaagagcg ccaccggcgt ccaggaggaa cagaccacat
       61 gcagcgaaaa accgaaggcc aaggtcccca ggagggcggc gggcgtcccg aacctctcat
      121 gccgtgccca aagccagaaa cccagcatgg cccaaaaatg gtgcagcacg atgagccagg
      181 cgaaggcgtc ggtcggcggc atcagtccgt aacaaagcca ccagggcgga taggcaaagg
      241 ccatctgggg gtccgccagg tagggcgttc cgaactgggc gtccgggttc cagagcggca
      301 gcaggccccg ccgcagggct tccgcgcccc atttccacaa tggataaaag aaccgggagg
      361 cgtccacaaa gacgaagatc ttcccgcccc aaaggacctg ccggtaaaag agggccgtca
      421 aggcggccag ggccaggggc acgccgaagg gggccgcccg cttccaggga tccatagggc
      481 cggccttcat ttcaggtccc ggcgtaaagc cgccaccacc ggcggcggca aggagaccat
      541 gtcccgttcc aggggggcca gtcccggggc gtccttccag cggtcatgaa cggccaaagc
      601 cgtccggaag gaatccctgg cctcggcgcg gccggccggg tccgaccgct ggcaatggcc
      661 caggttgacc caggccgcgg cgtagttggg ctccaattcg gcggcctttt tgaagtcggc
      721 ctcggcccgc gccggcccca gtcccgggac cggcttttcc agttttcggg cggcctcctc
      781 caggaaaagg ccttccccga aataaacgaa ggcgtccagg ggccgggctt gctcggccct
      841 gccccaggca tcccgggcgg ccgcggctga ggcgggaccc gggaattcct cgaaccgggc
      901 ggtccaacgc cgggcctcgc gctgggccca ggccccgtcc gccgcttcca aacgcagcac
      961 gccggcccag gcctcgtccg aacggctttc ccagaccggg tcacccgtcg ccaggtagag
     1021 ccgctccaag aacccggctt gggcggcctc cacccgcgcg tcccaggcgt ccaaccgcgc
     1081 cgcggtcgag agaccctcca gggcctcggg caggcggtgg cccgcctcca aggcctggga
     1141 ccgcgccacc agggacggcg tccaggccgc cgatccgaac aggcccagga ccaatcccaa
     1201 ccccaacaac cccccggccc aggagaaggg ggcccggccc cgggaaaccg gcgccaagag
     1261 ccccgcagcc tgcaatacga tggaaggggt atgcaggcag aaatcgaaga aggaagccgt
     1321 gcccaaggcc gtcaaggccg tggatctccc gttccctttc tcccggggcc aaagacggac
     1381 caacagggcc agcgcgagga cgcctcccac cagcccgaaa gccgtgagcc actccagggg
     1441 ttcgttgtgg gcgaattggg cgtcca
//
LOCUS       DASTJJ010000149         1837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1125200, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000149 DASTJJ010000000
VERSION     DASTJJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125200"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFR02_01830"
     CDS             complement(<1..355)
                     /locus_tag="VFR02_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET9869225.1"
                     /translation="MDWRLLQIADVAFPTGGFTLSGGLEAAAQLGEVRDEEALRGFLE
                     ASLRQAVRGALPLVERARREPGNFREVNALAQAFLTSPAANRSSRLQGSAFLTACEKA
                     FGPGGFVPLREGSDPW"
     gene            complement(364..>1837)
                     /gene="ureC"
                     /locus_tag="VFR02_01835"
     CDS             complement(364..>1837)
                     /gene="ureC"
                     /locus_tag="VFR02_01835"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET9869226.1"
                     /translation="GVYKADVGVKGGRIVGLGKAGNPDVMAGVTPGMVVGVTTEAIAG
                     EGLILTAGGIDAHIHFICPQQAEDALASGVTTLIGGGTGPATGTNATTCTPGSRHIGL
                     MLQATDALPMNFGFTGKGNTSSPEGLAEQIKAGAIGLKLHEDWGTTPAAIDNCLTVAG
                     EHDVQVTIHTDTLNESGYVDDSIAAFKGRSIHTYHTEGAGGGHAPDIIRVCGEPNVLP
                     SSTNPTRPYTVNTLDEHLDMLMVCHHLDKNIPEDVAFAESRIRGETIAAEDILHDLGA
                     LSMISSDSQAMGRVGEVICRTWQTASKMRSQRGALPEDKGGDDNFRARRYVAKYTINP
                     ALAHGVASEIGSVEKGKLADLVLWRPALFGVKPEMVIKGGFIAWAQMGDPNASIPTPQ
                     PLYMRRMFGAFGGAVGPTSLAFVSQAALAGKSLGKLDLTKTPVAVRDCRTVAKRHMKL
                     NDRLPKITVDPETYSVTADGERLTCGPAEKLPLAQRYFLF"
BASE COUNT          246 a          630 c          658 g          303 t
ORIGIN      
        1 cccagggatc cgacccctcc cgaaggggaa cgaacccccc cgggccgaag gccttttcgc
       61 aggccgtcaa gaacgccgag ccctgcaggc ggctggaacg gttggccgcc gggctggtga
      121 ggaaggcctg ggccagggcg ttcacttcgc ggaaattccc cggctcccgc cgcgcccgct
      181 ccaccagggg cagggcgccc cggaccgcct gccgcaggct ggcttcgagg aagccccgca
      241 aggcctcctc gtcccggacc tcgcccagtt gggccgcggc ctccaggccc cccgaaagcg
      301 tgaagccgcc cgtggggaag gccacgtcgg cgatctgaag caagcgccag tccatcccgc
      361 cccctaaaaa aggaaatacc gctgggccag cggcagcttt tccgcgggcc cgcaggtgag
      421 ccgctccccg tccgcggtga cgctgtaggt ctcggggtcc accgtgatct tgggcaggcg
      481 gtcgttgagc ttcatgtgcc gcttggccac cgtgcggcag tccctcacgg ccacgggcgt
      541 cttggtgagg tccagcttgc ccagcgactt gcccgccagg gccgcctggg acacgaaggc
      601 caggctggtg gggcccacgg ccccgccgaa ggcgccgaac atgcgccgca tgtagagggg
      661 ctggggcgtg gggatggagg cgttggggtc gcccatctgg gcccaggcga tgaagccccc
      721 cttgatcacc atctcgggct tcacgccgaa gagggcgggg cgccacagca ccaggtcggc
      781 cagttttccc ttttccaccg agccgatctc ggaggccacg ccgtgggcca gcgccgggtt
      841 gatggtgtac ttggccacgt agcgccgcgc gcggaaattg tcgtcgccgc ccttgtcctc
      901 gggcagggcg ccccgctggg agcgcatctt ggaggcggtc tgccaggtgc ggcagatgac
      961 ctcgcccacc cggcccatgg cctggctgtc gctggagatc atgctcaagg cccccaggtc
     1021 gtgcaggatg tcctcggcgg cgatggtctc gccccggatg cggctttcgg cgaaggccac
     1081 gtcctcaggg atgttcttgt ccaggtggtg gcagaccatg agcatgtcca ggtgttcgtc
     1141 gagcgtgttc accgtatagg gccgggtggg gttggtggag ctgggcagca cgttgggctc
     1201 gccgcagacc cggatgatgt cgggcgcgtg cccgccgccc gcgccctcgg tgtggtaggt
     1261 gtggatggac cggcccttga aggccgcgat ggaatcgtcc acgtagcccg attcgttcag
     1321 ggtgtcggtg tggatggtga cctgcacgtc atgttccccg gccaccgtga ggcagttgtc
     1381 gatggccgcc ggcgtggtgc cccagtcctc gtgcagcttg aggccgatgg ccccggcctt
     1441 gatctgctcg gccagcccct cgggcgagga ggtgttgccc ttgcccgtga agccgaagtt
     1501 catgggcagg gcgtcggtgg cctgcagcat gaggccgatg tgccggctgc cgggcgtgca
     1561 ggtggtggcg ttggtgcccg tggccgggcc cgtgcccccg ccgatgaggg tggtcacccc
     1621 gctggccagg gcgtcctcgg cctgttgggg acagatgaaa tggatgtggg cgtcgatgcc
     1681 gccggccgtg aggatcaagc cttcccccgc gatggcctcc gtggtcacgc ccaccaccat
     1741 gccgggggtc accccggcca tcacgtccgg gttgcccgcc ttgcccagcc ccacgatgcg
     1801 cccgcccttg acacccacgt cggccttgta gacgccc
//
LOCUS       DASTJJ010000150         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1492142, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000150 DASTJJ010000000
VERSION     DASTJJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492142"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFR02_01840"
     CDS             complement(<1..877)
                     /locus_tag="VFR02_01840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9869227.1"
                     /translation="MPTAGLLSNPLREGLSTDRVPLPCSLVIYGASGDLTARKLVPAL
                     YNLYKKHLLPTSFSLIGISRSKISDGDFKQKLKEMLKKAEPELTDALWNSFSQNFHYL
                     AGGYDEPAAYRRLAILLDEMDQQNGTSGNRVFYLSTPPNVFEPIISNLGAAGLNKEEK
                     GYSRVVIEKPFGHDLLSAKELNRKVQDVFTEHQIYRIDHYLGKETVQNLLVMRFANSI
                     FEPIWNRENVDHVQITAAEELGVGSRAGYYESSGVLRDMFQNHLFQVMCMVAMEPPVA
                     FEANAIRDEKLKILQS"
     gene            complement(1027..1734)
                     /locus_tag="VFR02_01845"
     CDS             complement(1027..1734)
                     /locus_tag="VFR02_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9869228.1"
                     /translation="MKVKSPPSKSGMRDRPENLTDRIYAQLKEDIFNFRLLPGDYFSE
                     NEVAERVSASRTPVREALTRLLRDGFVEVRSRSGWQVKPFDFNHFEQLYDLRIILEEA
                     AVRKLCEMELAPDFPELRERWLVKPEDRMAAGPAVAKMDERFHEQIVEAVGNQEMARI
                     HHDITERLRIIRRLDFTKPKRVDATYEEHAKILRAILKRRLEEATGLLTEHIKTSKGE
                     VRKITLHMLHEARSHRY"
BASE COUNT          300 a          550 c          639 g          358 t
ORIGIN      
        1 tggactgcag gatcttcagc ttctcgtcgc ggatggcgtt ggcttcgaag gccaccgggg
       61 gctccatggc caccatgcac atgacctgga acaggtggtt ctggaacatg tcccgcagca
      121 cgcccgagga ctcgtaatag cccgcccggc ttcccacgcc cagttcctcg gcggccgtga
      181 tctgcacgtg gtccacgttc tcccggttcc agatgggctc gaagatggag ttggcgaagc
      241 gcatgaccaa caggttctgc accgtctcct tgcccaggta atggtcgatg cggtagatct
      301 ggtgctcggt gaagacgtcc tgcaccttgc ggttgagctc cttggcggag agaaggtcgt
      361 ggccgaaggg cttctcgatg accacgcggg aatagccctt ttcctcctta ttcaagccgg
      421 ccgcgcccag gttggagatg atgggttcga agacgttggg gggcgtggac aggtagaaga
      481 cccggttgcc cgaggtgccg ttctgctggt ccatctcgtc gagcaggatg gccaggcgcc
      541 ggtaggccgc gggctcgtcg tagccgcccg ccaggtaatg gaagttctgc gagaagctgt
      601 tccacagggc gtcggtgagc tcgggctcgg cctttttcag catctccttg agcttttgct
      661 tgaagtcgcc gtcggagatc ttggagcgcg agatgccgat gaggctgaag gaggtgggca
      721 gcaggtgctt cttataaagg ttgtagagcg cgggcaccag cttgcgggcg gtcaggtcgc
      781 ccgaggcgcc gtagatgacc aggctgcagg gcagggggac ccggtcggtg gaaagccctt
      841 cccgcaaggg attggacagg aggccggcgg ttggcatggg ggaacctcga attgggatgg
      901 ggtattttac ggaataaccc gacaaacatc caaggatgat tcgggcgaat tcgcgccccc
      961 agcgggccag actatttttc caggcaaaaa aggcccgggc ggggggcccg gggggatggg
     1021 ggcgggctag tagcggtggc tgcgggcctc gtggagcatg tgcagggtga tcttgcgcac
     1081 ctcgcccttg ctggtcttga tgtgctcggt caagaggccc gtggcctcct ccaaccggcg
     1141 cttgaggatg gcgcgcagga tcttggcgtg ctcctcatag gtggcgtcca cccgcttggg
     1201 tttggtgaaa tccagccggc ggatgatgcg caggcgctcc gtgatgtcgt ggtggatgcg
     1261 ggccatttcc tggttgccga cggcctccac gatctgctcg tggaaccgct cgtccatctt
     1321 ggccaccgcc gggcccgcgg ccatccggtc ctcgggcttc accagccagc gctcgcgcag
     1381 ctcggggaaa tcgggcgcca gttccatctc gcagagcttc cggaccgcgg cctcctccag
     1441 gatgatgcgc aggtcgtaga gctgctcgaa gtggttgaag tcgaagggct tcacctgcca
     1501 gccgctgcgc gagcgcacct ccacgaaccc gtcgcgcagg agccgggtca gggcctcccg
     1561 cacgggcgtg cggctggccg acacccgctc ggccacctcg ttctcgctga aataatcccc
     1621 gggcagcaac cggaaattga agatgtcttc cttgagttgg gcgtagatgc ggtccgtgag
     1681 gttctccggg cggtcccgca tgccggactt ggaggggggg gatttgactt tcataatttc
     1741 ctttcattgt agcaccgacc gaacctttta caaacgcccc gccgccgggc cgtcgggccg
     1801 caccaccacc agggggtcgc cggcggccac cggcgagccg ggccggc
//
LOCUS       DASTJJ010000151         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_268663, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000151 DASTJJ010000000
VERSION     DASTJJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268663"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFR02_01850"
     CDS             complement(<1..247)
                     /locus_tag="VFR02_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9869229.1"
                     /translation="MAEKSVIQVGMLGFGNVGQGLYRLLEKNRTLIEGKIGAQLKIKK
                     ILVRDPAKPREVPLPEGLLTSNFDEILNDPEIDVVLEL"
     gene            complement(369..>1182)
                     /locus_tag="VFR02_01855"
     CDS             complement(369..>1182)
                     /locus_tag="VFR02_01855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9869230.1"
                     /translation="PSPYWVTYPEQVKMAGGVPVYVECSEADGFKLTAAALKAKITPK
                     TKVLLLNSPSNPTGAVIGRKTLEGIAELALAHKLWVISDEIYAKLIYGEPHTCFPSLS
                     KEVAAQTILINGMSKAYAMTGWRIGYMAGPSAVLKAVADFQSHSTSNPSSISQKAAVA
                     ALSIPDAEIQKMVAVFRKRRDLIVDGLNAIPGVKCLRPAGAFYVFPNVTGVFKPGRAN
                     SMELSDFLLEKAKLALAPGIAFGAEGYLRLSYATGEKTIEEGLKRFRETVAS"
BASE COUNT          207 a          356 c          393 g          226 t
ORIGIN      
        1 tcagctccag caccacgtcg atctcggggt cgttgaggat ctcgtcgaaa ttggacgtga
       61 gcagcccctc gggcaggggg acctcccggg gcttggcggg gtcgcgcacc aggatcttct
      121 tgatcttgag ctgggcgccg atcttgccct cgatgagggt ccggttcttc tccaaaaggc
      181 ggtacaggcc ttggcccacg ttgccgaagc ccaacatgcc cacttgaatg accgattttt
      241 cagccatgcc gtcccctggg ttcaccggaa tagaaaacgc ccgggacccg gggtccaggg
      301 cgctttgaag cgagtgcaaa tagtaacgaa aaaacccgtt gttttcaacg tggattcccg
      361 gcgcggggtc aggacgccac ggtctcccta aagcgcttca ggccttcctc gatggtcttt
      421 tcgcccgtgg cgtaggaaag cctcagatag ccctccgcgc cgaaggcgat gccgggcgcg
      481 agcgccagtt tggccttttc cagcaggaaa tccgacagct ccatggagtt ggcgcggccg
      541 ggcttgaaga ccccggtcac gttggggaac acgtagaagg cgcccgcggg cctcaggcac
      601 ttcacgcccg ggatggcgtt gaggccgtcc acgatcaggt cccggcgttt ccggaagacc
      661 gccaccatct tctggatctc ggcgtcgggg atcgagaggg ccgccacggc ggccttctgg
      721 gagatggagg aggggttgga ggtggaatgg ctctggaagt cggccaccgc cttgaggacg
      781 gccgagggcc cggccatgta gccgatgcgc cagcccgtca tggcgtaggc cttggacatg
      841 ccgttgatga ggatggtctg ggccgccact tccttcgaga ggctcgggaa acaggtgtgc
      901 ggctccccat agatgagctt ggcgtagatc tcgtccgaga tcacccaaag cttgtgggcc
      961 agggccagtt cggcaatgcc ttccagggtc ttgcggccga tcacggcgcc ggtcgggttg
     1021 ctgggggaat tcagcagaag caccttcgtc ttgggcgtga tcttggcctt caaggccgcc
     1081 gcggtcaact tgaagccgtc ggcttccgaa cattccacgt agaccgggac gccgccggcc
     1141 atcttcacct gttccggata ggtcacccaa tagggcgagg gg
//
LOCUS       DASTJJ010000152         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_268757, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000152 DASTJJ010000000
VERSION     DASTJJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268757"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFR02_01860"
     CDS             <1..527
                     /locus_tag="VFR02_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9869231.1"
                     /translation="LVGAGTALKDRPRLSVRLPGYGRSDGWPRRVLLDSGLRVPPGSP
                     LFQGPQKTLVFCSPRASAAVEKALRRRGAEVFRVARGPQGLSLKEVLARLQGEGVRSL
                     MVEGGAAVHGSFLDARLADGVALFLAPKLFGGTAPAWIGGAGFPDPDRAPRLTGTRLE
                     SLGVDHLLTGKVRY"
     gene            529..1128
                     /locus_tag="VFR02_01865"
     CDS             529..1128
                     /locus_tag="VFR02_01865"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET9869232.1"
                     /translation="MFTGLVQELGVVRSAQRRGADLWLVLEAPLTAPKLRLGDSVAVN
                     GCCLTAVEVQPPLLSFQAVPETLSRTLLGELKEGDRVNLELPLTPSQPLGGHFVQGHV
                     DGTAEITAKEPEGQGLRLTLRLPDGLSRYVVEKGSLALDGTSLTVAALKGNDVEIALI
                     PHTVENTVFGRKGPGDRVHVEVDLLAKYVERLAPAAPRP"
     gene            1142..1579
                     /locus_tag="VFR02_01870"
     CDS             1142..1579
                     /locus_tag="VFR02_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HET9869233.1"
                     /translation="MDLKVNRVGDPDPSQPDPLKGVFSPALNDRISKVADPALRSELA
                     ALIVEVEDKSRKLLSTRGNKDFEDYKAAVKRFVKRAVQSSFKVEEKQSQKRDGKFVVY
                     LTVEKVDEALENLAQLLVAGQQSPMRVVATLDEIRGMLMDFYL"
BASE COUNT          273 a          540 c          599 g          277 t
ORIGIN      
        1 tcctggtggg ggccgggacc gccttgaagg acaggccgcg cttgagcgtg cggctgcccg
       61 gttacggccg gtcggacggc tggccccggc gggtattgtt ggattcgggc ctgcgggtgc
      121 ccccgggctc gcccctcttc cagggccccc agaaaaccct ggttttctgc tccccccggg
      181 cttcggcggc ggtggaaaag gccctgcgtc ggcggggcgc ggaggtcttc cgggtcgccc
      241 ggggccccca agggctctcc ttaaaagaag tgctggcccg tttacagggg gaaggcgtcc
      301 ggtctttaat ggtggaaggc ggcgcggcgg tgcatgggtc cttcctggac gcgcggctgg
      361 ccgacggggt ggcgctcttc ctggcgccca aacttttcgg gggcaccgcc cccgcctgga
      421 tcggcggcgc gggttttccc gaccccgacc gcgcgccccg tttgacgggg acgcggctgg
      481 aaagcctggg agtggatcat cttttgaccg gaaaggttcg gtactgactt gttcaccgga
      541 ttggtgcagg aactcggcgt ggtgcggtcc gcccagcggc ggggggcgga cctttggctc
      601 gtcctggagg cgcccttgac cgcgcccaag ctgcgcctgg gcgacagtgt ggccgtcaac
      661 ggctgctgcc tgacggcggt ggaggtccag ccccccctct tgtccttcca ggccgtgccc
      721 gagaccctgt cccgcaccct cttgggggaa ttgaaggaag gggaccgggt gaacctggag
      781 ttgccgctca ccccctccca gcccctgggc gggcatttcg tgcagggaca cgtggacggc
      841 acggccgaga tcacggccaa ggaacccgag ggacagggcc tgcgcctgac cctgcgcctg
      901 cccgacggcc tgtcccgcta cgtggtggaa aagggcagcc tggccctgga cggcaccagc
      961 ctgaccgtgg cggccctcaa ggggaacgac gtggagatcg ccctcatccc ccacacggtg
     1021 gagaacacgg tgttcggccg caagggcccg ggggaccggg tgcacgtgga agtggacctg
     1081 ttggccaaat acgtggagcg gctggccccg gccgccccgc gcccatgacc ggggggaccc
     1141 catggacctg aaagtgaacc gggtcggcga cccggaccct tcccaacccg accccctgaa
     1201 gggcgtcttt tccccggcct tgaacgaccg catctcgaaa gtggccgacc cggccttgag
     1261 aagcgaactg gccgccctta ttgtggaggt cgaggacaag agccgcaagc tgctctccac
     1321 ccggggcaac aaggatttcg aggactacaa ggccgcggtg aagcgcttcg tcaagcgggc
     1381 cgtccaaagc tccttcaagg tcgaggaaaa acagtcccag aagcgggacg gcaagttcgt
     1441 ggtctacctg accgtggaaa aggtggacga ggcgctggag aacctggccc agttgctggt
     1501 ggcgggccaa cagtcgccca tgcgggtggt ggccaccctg gacgagatcc gcggcatgct
     1561 gatggacttt tatttgtaaa aggctgaaag cccgttcacc gcaaaggcgc caagtccgcc
     1621 aaggaaagcg aaggacgggg accccgattt cggggatgga tcttttggat cgagcctttc
     1681 ttggcggac
//
LOCUS       DASTJJ010000153         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_391645, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000153 DASTJJ010000000
VERSION     DASTJJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391645"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="aroA"
                     /locus_tag="VFR02_01875"
     CDS             <1..991
                     /gene="aroA"
                     /locus_tag="VFR02_01875"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9869234.1"
                     /translation="LLAGQPFTSRLTGDKYLRRRPMKRVVGPLSLMGARISGREGADH
                     LPLTVAPRKLKGIQYRLPIASAQVKSAILMAGLFAEGVTRVTEPAPSRDHTERMFQAF
                     GIPLKRRGTTAFLKGPVAPFRARKILVPGDISSAAFFLVAGLIVPGAKLVLKNVGMNP
                     TRTGLVEVLRKMGGRIRIFPHRTAPGTEPTADLLVQGSALKGVVVEGEMIPRLIDELP
                     VLAAAATQARGLTVVRNAEDLKVKESDRILMMAMTLGKMGADIEPQPDGWVIKGPTPL
                     SGARLSSGGDHRVAMSLAVAALIAKGTTTILDTENINTSFPGFEKALKALVHS"
     gene            1064..>1622
                     /gene="cmk"
                     /locus_tag="VFR02_01880"
     CDS             1064..>1622
                     /gene="cmk"
                     /locus_tag="VFR02_01880"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9869235.1"
                     /translation="MGSVIIVAIDGPAGAGKSTVAKRLAKKLGFLHIDTGAMYRAVTL
                     AALRQKADLTSDAELSRIAEAARIELRPGDPDNKVFLNEEDVSQEIRTPEVTRATAYI
                     ANSVPVRKILVARQQQMGRVDSAPYGGAVLEGRDIGTDVFPDARFKFFLDASVETRAQ
                     RRLLELQKAGVETTLEKVMADVAARD"
BASE COUNT          281 a          528 c          565 g          248 t
ORIGIN      
        1 cctattggcc ggccaaccct tcaccagccg cctgacgggg gacaagtacc tgcggcgtcg
       61 gcccatgaaa cgggtggtgg ggccgctctc cctgatgggc gcccgcattt cggggcgcga
      121 aggggccgac cacctgccgc tcaccgtggc gccgcgcaag ctcaagggga tccaataccg
      181 cctgcccatc gccagcgccc aagtgaagag cgccatcctg atggccgggc ttttcgccga
      241 gggggtgacc cgcgtcaccg aacccgcccc cagccgggac catacggaac gcatgttcca
      301 ggccttcggc atcccgctca agcgccgggg gacgacggcc ttcctgaagg gcccggtcgc
      361 gccctttcgg gcccgcaaga tcctggtgcc gggggacatc tcgtcggcgg catttttcct
      421 ggtggcgggc ctcatcgtgc cgggggccaa actggtgctg aagaacgtgg gcatgaaccc
      481 cacccgcacg ggcttggtgg aggtgctgcg caagatgggg gggcgcatcc ggatcttccc
      541 ccaccggacc gcgccgggga ccgaacccac cgccgacctg ctggtgcagg gctcggccct
      601 gaagggcgtg gtggtggagg gggagatgat cccccggctg atcgacgaac ttcccgtgct
      661 ggcggcggcc gccacccagg cccgcggcct gaccgtggtg cgcaacgccg aggacctgaa
      721 ggtcaaggag tcggaccgca tcctgatgat ggccatgacg ctcgggaaaa tgggggcgga
      781 catcgagccc caacccgatg gttgggtgat caagggcccc acgcccctgt cgggcgcccg
      841 gctttcctcg gggggggacc accgggtggc catgtccctg gcggttgccg ccttgattgc
      901 gaagggaact acgactattt tagacacgga aaacatcaac acgtcgttcc cgggctttga
      961 gaaggccttg aaggcgctgg tgcactcttg accggcccgc ggacgcggcg ctttgaagga
     1021 cggccggtct agacgccggc atttatcacg aaaggtggat cgaatgggtt cagtcatcat
     1081 tgtggcgatc gacggccccg cgggagcggg caagagcacg gtggccaagc gtctcgccaa
     1141 aaagctgggg ttcctccata tcgacaccgg ggccatgtac cgggcggtca ccttggcggc
     1201 cctgcggcag aaggccgacc tgacgagcga cgccgaattg tcccgcatcg ccgaggcggc
     1261 gcgcatcgaa cttcgcccgg gggaccccga caataaagtt tttttaaatg aggaagacgt
     1321 ttcccaagag atccgcacgc ccgaagtgac ccgggccacc gcctatatcg ccaactcggt
     1381 gccggtgcgc aagatcctgg tggcccgcca acagcagatg ggccgggtgg acagcgcgcc
     1441 ctacgggggc gccgtgttgg aaggccgcga catcggcacg gacgtctttc cggacgcgcg
     1501 gttcaagttc ttcctggacg cgtcggtgga gacccgggcc cagcgccgcc tgctggaact
     1561 gcagaaggcg ggcgtggaga ccacgctgga aaaggtcatg gcggacgtgg cggcccgcga
     1621 cg
//
LOCUS       DASTJJ010000154         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1492440, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000154 DASTJJ010000000
VERSION     DASTJJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492440"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>1020
                     /locus_tag="VFR02_01885"
     CDS             26..>1020
                     /locus_tag="VFR02_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869236.1"
                     /translation="MAGLWAASLGPWLLKNWVYTGNPLYPYFSGLLGGLRLPADRYAE
                     LLGNHESAFGTGMPFWRWPLEAWRHLDPTLAPPLLGFLPFLLLGAVFWKKERFLLSLA
                     ALSLAGGFLVSFQTRLMVPEMFLFLTAAGCFIGRWRPADKTTGGLGAAVAPRAWAAVL
                     SLFGLLTLLSVARLTVQYDQSQLIWLGAQSRDAYLRACPQTASYYSLARACGWLPEGD
                     RLLIIGDARALYYPRPFLVNSVYDVPLWTRIGRSSPDPAAVGEALRRLGVDDLVFSGE
                     EQARMAGLYPADYALTPAQARVLDGFLEKRTQRVYQDGPRAIYRLKPPAAPRGKPG"
BASE COUNT          121 a          376 c          329 g          194 t
ORIGIN      
        1 cgagcggtgg aaggactggg ctctggtggc cgggctctgg gcggcctcct tgggtccctg
       61 gctcttgaag aactgggtct acaccggcaa tccgctctat ccctatttct ccggcctcct
      121 gggcggcctg cgccttccgg cggaccgtta cgccgaactg ttgggcaacc acgagtccgc
      181 cttcggcacg ggcatgccct tctggcgttg gccgttggag gcctggcggc acctggaccc
      241 gaccctggcg ccgcccctcc tggggttcct gcccttcctc ctcctgggcg cggtcttttg
      301 gaaaaaagaa aggttcctgc tttccttggc cgccctttcc ctggcggggg gattcctggt
      361 ctccttccag acccggttga tggtgccgga aatgttcctg ttcctgaccg ccgccggttg
      421 tttcatcggc cgttggcggc ccgccgataa aacaaccggc ggcctggggg ccgccgtcgc
      481 cccacgcgcc tgggcggcgg tcctttcgct cttcggcctg ctcaccctcc tatcggtcgc
      541 gcgcctcacc gtccagtacg accaaagcca attgatctgg ctgggcgccc aaagccgcga
      601 cgcctacctg cgggcctgcc cccagacggc ttcttattat tccctggccc gggcctgcgg
      661 atggctgccg gagggcgacc ggctgctcat catcggcgac gcccgggcgc tctattaccc
      721 ccgccccttc ctggtcaaca gcgtctatga cgtgcccctc tggacgcgga tcggacggtc
      781 atcccccgac ccggcggcgg tgggggaggc cttgcggcgc ctgggcgtgg atgacctggt
      841 cttttcgggt gaggagcagg cccgcatggc cgggctctac ccggcggatt acgccctgac
      901 ccccgcccag gcccgggtcc tggacggctt cctggaaaag cggacccagc gggtctacca
      961 ggacggcccc cgcgccatct accggctcaa gccgccggcc gccccccggg ggaagccggg
//
LOCUS       DASTJJ010000155         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1492454, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000155 DASTJJ010000000
VERSION     DASTJJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492454"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFR02_01890"
     CDS             <1..877
                     /locus_tag="VFR02_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9869237.1"
                     /translation="QIHHLSARQKKPFITVHCGSIQRGQFDSELFGLERQDARGRTSP
                     QIGQLEFANEGTLFLEQADRLPDEVQEKLLRVFHEKKMTRADHTVTIPIDIRVIASLE
                     TDQENKPRDTDLRGDFLQFLNGFPLVVPPLRDRKEDIPELIEHFLRQVNRKARMTVKS
                     LSKEAVQFFAHYSWPGNLQELENSIEMMVLFAGREVLTLEDIPLDILVKQIDLAKTKE
                     EAKLSLKRVRHQFERHYIRKVLERTRGNQTRTAATLGLHRNTLIWKLKELDLEDDYKK
                     IVKKRRERGLGFRNL"
     gene            954..>1142
                     /locus_tag="VFR02_01895"
     CDS             954..>1142
                     /locus_tag="VFR02_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869238.1"
                     /translation="MTVFQDLLNSLPSGPAPTPDFSRAEKILQEHLSASRARYLPAAD
                     WKAHPAWRRLSRGGTWEAG"
BASE COUNT          232 a          401 c          340 g          169 t
ORIGIN      
        1 gcagatccac cacctgagcg cccggcagaa gaagcccttc atcaccgtgc attgcggcag
       61 catccaacgc ggccagttcg acagcgaatt gttcggcctg gagcgccagg acgcccgggg
      121 ccgcacctcc ccccagatcg gccagttgga attcgccaac gaaggcaccc tcttcctgga
      181 acaggccgac cgcctgcccg acgaggtcca ggaaaaattg ttgcgggtct tccacgagaa
      241 aaaaatgacc cgggccgacc acacggtcac catccccatc gacatccggg tcatcgcctc
      301 cctggaaacg gaccaggaga acaaaccccg ggacaccgac ctgcggggcg acttcctgca
      361 gttcctcaac ggcttccccc tggtggtgcc gcccttgcgg gaccgcaagg aggacatccc
      421 ggagctcatc gagcatttcc tccggcaggt gaaccgcaag gcccgcatga cggtcaagag
      481 cctctccaag gaggccgtgc agttcttcgc gcactacagc tggccgggga acctgcagga
      541 gttggagaac agcatcgaga tgatggtgct cttcgcgggc cgcgaggtcc tgaccctgga
      601 ggacatcccc ctggacatcc tggtcaagca gatcgacctg gccaagacca aggaagaggc
      661 caagttgtcc ctcaagcggg tccgccacca gttcgagcgg cattacatcc gcaaggtgct
      721 ggaacgcacg cgcggcaacc agacccgcac ggccgccacc ctggggctgc accgcaacac
      781 cctcatctgg aagctcaagg aactggacct ggaagacgac tacaagaaga tcgtcaagaa
      841 gcgccgggaa cggggcctgg gtttccgcaa cctttgacgt cccccggccg ccctccccgc
      901 cggatgcccg gcggggacaa gccatcttcg cttgggagtg acgtttggac cgcttgaccg
      961 tcttccaaga cctgctcaac agccttccct cggggcccgc cccgaccccc gatttttccc
     1021 gcgccgagaa gatcctccaa gagcatttga gcgcgtcccg ggcgaggtac ctgcccgcgg
     1081 ccgactggaa ggcccatccg gcctggcggc ggctttcccg gggagggacc tgggaagccg
     1141 gc
//
LOCUS       DASTJJ010000156         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_758231, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000156 DASTJJ010000000
VERSION     DASTJJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758231"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VFR02_01900"
     CDS             complement(<1..1630)
                     /locus_tag="VFR02_01900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9869239.1"
                     /translation="MNKTCLYIAVVGVAIILAAPARGETPGSSPTPGPTAPPTPLPTP
                     TATFTPVPEPDYSAAVSNLDTYLRDRLGKDNVKGFSLALVDGDRVVWVKGYGRVDPDR
                     EGPAAGDTRYRLGAASELFTAVEAMRWVERGSLDLDAPLTAALPGFSIQSRFPKAKPI
                     TPRALLAQHSGLPGFFYQGMWADPGQSLAGLAADLRRDYLVAPPQTLYRYSYLDFDLL
                     GRIVELKSGKPFAAALRSDLFHPLGMEDSFFGEDPGSDLPPAPPCRDGHPIPPLHYRD
                     VPAVGMVSTANDLAKFLRFVLGGAADPGPAPLDRTTRASMLLPQYPGLPLDFGQSLGL
                     GWRLSGLTVFGAGEAAWRGGEYPGYTSQVAILPDQKLGVAFLSNSNEADKLAGQAVQR
                     AFQLMLQAKYGKTPVLERPKPRMPPVAAVPTETLDRVAGDYSALGQLVRIARQDNHLT
                     AAFAKYRVDLLPVDQQTFVPHLVFLLLFPVDLPQYPLTFSTAGDQAVATLGGLPFVLP
                     LEKIEPVPIPDAWRAREGDYVQENPEPQLQFGSIR"
BASE COUNT          279 a          554 c          594 g          247 t
ORIGIN      
        1 ggcggatgga cccgaattga agttggggtt cggggttttc ctgcacatag tccccctccc
       61 gggcccgcca ggcgtccggg atgggcacgg gctcgatctt ttccaggggt aggacgaagg
      121 gcaggccgcc cagggtggcc accgcttggt ccccggccgt cgagaaggtc agcggatatt
      181 ggggcaggtc cacggggaac aacaacagga agaccaggtg cgggacaaag gtctgctggt
      241 cgacggggag caggtccacc cggtatttgg cgaaggccgc cgtcaggtgg ttgtcctgcc
      301 gggcgatgcg caccaactgc cccaaggccg aatagtcccc cgcgacccgg tccagcgtct
      361 cggtgggcac cgccgccacc ggcggcatgc ggggtttggg cctttccaac accggggtct
      421 tcccgtactt ggcctgcagc atgagttgga aggcccgttg caccgcctgt cccgccagct
      481 tatccgcctc gttgctgttg gacaggaagg ccacccccag tttttgatcc ggcaggatgg
      541 ccacctggct ggtgtagccc gggtattccc cgccgcgcca ggcggcctcg cccgcgccga
      601 agaccgtgag cccgctcagg cgccaaccca accccagcga ttgcccgaaa tccagcggca
      661 ggccgggata ctggggcagg agcatggagg ccctcgtcgt tcggtccaaa ggcgccggtc
      721 cggggtccgc ggcgcccccc aggacgaacc ggaggaactt ggccaggtcg ttggccgtgg
      781 agaccatgcc caccgcgggc acgtcgcggt aatgcagcgg cgggatcgga tgcccgtccc
      841 ggcagggcgg cgccggcgga agatcggatc ccgggtcctc cccgaagaag gaatcctcca
      901 tccccagggg atggaagagg tccgaccgga gggcggccgc gaagggcttg ccggacttca
      961 attccacgat ccggcccagg aggtcgaaat ccaggtagga atagcggtaa agggtctggg
     1021 gcggcgcgac caggtaatcc cgccgcaggt ccgccgccag cccggctagg ctctggcccg
     1081 ggtcggccca catgccttgg tagaagaacc cgggcaatcc cgagtgctgg gccagcaggg
     1141 cccggggggt gatgggcttg gctttgggga accggctctg gatggagaac cccggcaacg
     1201 cggcggtcaa gggcgcgtcc aggtcgagcg aaccccgttc cacccaccgc atggcctcca
     1261 ccgcggtgaa aagctcggaa gccgcgccca agcggtagcg cgtgtccccg gcggcgggcc
     1321 cttcccggtc cgggtccacc cgcccgtaac ccttcaccca gaccacccgg tccccatcca
     1381 ccagggccag gctgaaaccc ttcacattgt ccttgcccaa gcggtcccgc agataggtat
     1441 ccaggttgga aacggcggcc gaataatcag gctcggggac gggcgtgaag gtggcggtgg
     1501 gcgtgggcaa gggcgtcggc ggggcggtcg gccccggcgt ggggcttgaa cccggggtct
     1561 ccccacgggc cggggcggcc aagatgatgg ccaccccgac cacggctata tagaggcaag
     1621 ttttgttcat ggacgccttt gagggggatt cgcccggcag cagcattcgc ctcc
//
LOCUS       DASTJJ010000157         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_269289, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000157 DASTJJ010000000
VERSION     DASTJJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269289"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1831
                     /locus_tag="VFR02_01905"
     CDS             <1..>1831
                     /locus_tag="VFR02_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869240.1"
                     /translation="SDEMAVTPEGLDIPEAPGEETPTPTPTPEEETELKIPDQHLFNF
                     QLPAESQLSIQGYKQIEADLKFQHFNRPDLNGVPASNNTTDVNQQLVVNLDGKVGQNV
                     DVHVNYSDVNRTGGLDQTQQEISIVYHGNQDSPVQEVSFGDLNLLLPNTEFAGFNKQL
                     FGIQAKLKFDDFRLTSFFAQTKGLTETKIFKGNQVQVNLTLNDTQYVPYKYFLITKTS
                     EPATVNGQVVNQALPRPNSEQVWVDPGNGQVPPQSPNYIGPFQRFLPGRDYTIDYNSG
                     IITFLTPVGIGSRIAVAFVQLDGTRIGEDAAGNIQDLNNPSSLSVPADGRMLPNSTAH
                     LIKNNVNPAQVSPLYLVNVYSLGTVPIVPPNQDPNFQFQIINQGTNNVVQTGDPPGAR
                     WAITVDSNLNLLLVTDTQNPDFPERPFANPAPDTTNGTGPNDVYSQTTPPTSLYRMQL
                     LYDTKQDYFNMGRFNILRGSESVYLDGRLLHRDTDYYFDYTTGNLDFQDKNLLRPDSQ
                     VVVTYEYSPFGPFAQENILGTRAEYDVTDHFFLGSTFLYSGSQQPADVPQMGGEPNDL
                     ALLDADASLDLDRASVKSLTSLIPGLEDWTPPLSVKLSGEVAKS"
BASE COUNT          390 a          663 c          512 g          266 t
ORIGIN      
        1 tcggacgaga tggccgtgac gcccgagggc ctggacatcc ccgaggcccc gggcgaggag
       61 accccgaccc ccacgcccac ccccgaggaa gagaccgaat tgaagatccc cgaccaacac
      121 ctcttcaact tccagcttcc ggcggaaagc cagctgtcca tccagggata caagcagatc
      181 gaggcggacc tcaagttcca gcacttcaac cggcccgacc tgaacggcgt gccggcctcc
      241 aacaacacca cggacgtcaa ccagcagttg gtggtgaacc tggacggcaa ggtggggcag
      301 aacgtggacg tgcacgtgaa ctacagcgac gtcaaccgca cgggcggcct ggaccagacc
      361 cagcaggaga tctccatcgt gtaccacggc aaccaggaca gcccggtgca ggaggtgtcc
      421 ttcggcgacc tgaacctgct cctgcccaac accgagttcg cgggcttcaa caaacagctc
      481 ttcggcatcc aggccaagct gaagttcgac gacttccggc tcacgtcctt cttcgcccag
      541 accaagggcc tgaccgagac caagatcttc aaagggaacc aggtgcaggt gaacctgacc
      601 ttgaacgaca cccaatacgt gccctacaag tacttcctca tcaccaagac ctccgagccg
      661 gccaccgtca acggccaggt ggtgaaccag gccctgcccc ggcccaacag cgagcaggtc
      721 tgggtggacc cgggcaacgg ccaggtgccg ccgcaaagcc ccaactacat cggccccttc
      781 cagcggttcc tgccggggcg ggactacacc attgactaca actcggggat catcaccttc
      841 ctcacgccgg tcggcatcgg gtcccgcatc gcggtggcct tcgtccaact ggacggcacc
      901 cgcatcgggg aggacgcggc gggcaacatc caggacctga acaacccctc cagcctgtcc
      961 gtgccggcgg acgggcgcat gctgcccaat tcgaccgccc acctgatcaa gaacaacgtg
     1021 aaccccgccc aggtcagccc cctgtacctg gtgaacgtct acagcctggg gaccgtcccc
     1081 atcgtgcccc cgaaccagga ccccaacttc cagttccaga tcatcaacca ggggaccaac
     1141 aacgtggtgc agaccgggga ccccccgggg gcgcgctggg ccatcacagt ggacagcaac
     1201 ctgaacctgt tgttggtgac cgacacccag aaccccgatt tccccgagcg gcccttcgcc
     1261 aatccggcgc ccgacaccac caacggcacg ggccccaacg acgtctacag ccagaccacc
     1321 ccgcccacca gcctctaccg catgcaactg ctctacgaca ccaagcagga ctacttcaac
     1381 atggggcgct tcaacatcct gcgggggagc gaatcggtgt acctggacgg gcgcctgctg
     1441 caccgggaca cggattatta ttttgactac accacgggca acctggactt ccaggacaag
     1501 aacctgctgc gtcccgacag ccaggtggtg gtcacctacg aatattcgcc cttcggcccc
     1561 ttcgcccagg agaacatcct ggggacccgg gccgaatacg acgtgaccga ccactttttc
     1621 ctggggtcca ccttccttta cagcggttcc cagcagcccg ccgacgtgcc ccagatgggc
     1681 ggggagccca acgacctggc cctgttggac gccgacgcca gcctggacct ggaccgggcc
     1741 tcggtgaagt cgctcactag cctcatcccg gggctggagg actggacgcc gcccttgagc
     1801 gtcaagctgt cgggggaagt ggccaagagt t
//
LOCUS       DASTJJ010000158         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1370580, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000158 DASTJJ010000000
VERSION     DASTJJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370580"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFR02_01910"
     CDS             <1..1607
                     /locus_tag="VFR02_01910"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9869241.1"
                     /translation="FWGALGAVLLALNLRLLTDRFFGGLASSWARRLLLLGPALALAF
                     SRTYWAESISTKGGIYILESLLLMALFLSLLRSESGPPARFDRGTAAAFFLAGLGLSH
                     HWQTQLIFLPGLFLFVLVRGGGRLPPAKAWFQNLAWTALGLSVLGLYLPLRANLQPVL
                     DLGDPQTLFLFKMAVTRGYVQGEEPSLLAALARALTGAGPWEPVRRIWDAMMENQLTG
                     IVPHFWGDLKPTLLALAGFGVWAWARRGAKAALVFLLCGLGCLLSVFYTGLLVPTVAG
                     RWTLDNYLLPSNWITALLGGLGLLALWGPLGKARAGAGALKAAWCLLAAALPLWPLLS
                     NFPPNDESRQMLRYDYGVNLMKSTPRGAILFSEGDEDFFPLYYLQLVEHRRPDLCVIP
                     PFTLFETWGVAELERFHPELGLTAAGKDFPDHFRRIEYALPEIVDKNRSKRVIAFTYL
                     SGAFHRYYCQPLGVKKAESSGILLWLDGPLNRRFKPLPREGLRLRHWWDSPSNAHPSL
                     RRVLWAYLQMGVRPPGAPLQPPSGRP"
     gene            1641..>2734
                     /locus_tag="VFR02_01915"
     CDS             1641..>2734
                     /locus_tag="VFR02_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HET9869242.1"
                     /translation="MPPKPSTLGELKASGYPDLTVKQELEKNLLKKLKAGEPLFPQIH
                     GFEETVVPQLTNAILSHHDIVLLGEKGQAKSRLMRAIAQFLDEEIPVVRGCEIHDHPY
                     HPICAACRKKLAAQGDGLEVEWIGREARYGERLAPGTKIADLIGDLDPAKVAAGNPLA
                     SEEALHFGLIPRMNRGIFAVNELPDLEYLVQVALFNILEERDVQVRGFPIRFDLDLLL
                     LFTANPEEYTRAGKIITQLKDRVGAEIRTHYPLTRELGIKIIHQEAAPPLDDGLTLTV
                     PDFMVEIIEQITLEARRSPFVNQKSGVSARLSIANYETMVANARRRAYLLGEKEAVPR
                     MSDLNYLYTSSSGKIELDPFREETVTEFQV"
BASE COUNT          409 a         1004 c          836 g          485 t
ORIGIN      
        1 ccttttgggg ggccctgggc gccgtgctct tggccctgaa cctgcggctt ttgacggacc
       61 gttttttcgg gggcctggcc tcctcctggg cccggcgcct gctgctcctc ggccccgccc
      121 tggccctggc cttttcccgg acctattggg ccgaatccat cagcaccaag ggcggcatct
      181 acatcctgga atcgctgctc ttgatggcct tgttcctcag cctgctgagg tccgagtccg
      241 gtccgcccgc ccgcttcgac cgggggacgg cggcggcctt tttcctcgcg ggcctgggcc
      301 tgtcccacca ttggcagacc cagctcatct tcctgcccgg cctcttcctt ttcgtcctcg
      361 tgcggggcgg cggccgcttg cccccggcca aagcctggtt ccaaaacctc gcctggacgg
      421 cgctggggct ttcggtcctg ggcctttacc tgccgctgcg cgccaacctg cagcccgtgc
      481 tggacctggg cgatccccag acccttttct tgttcaagat ggccgtgacc cggggctatg
      541 tgcagggcga ggaacccagc ctgctggccg ccctggcccg ggcgctgacc ggcgccgggc
      601 cctgggaacc cgtccgccgc atctgggacg ccatgatgga gaaccagctg accggcatcg
      661 tgccccattt ctggggggac ctgaaaccca cgttgctggc cctggccggt ttcggggttt
      721 gggcctgggc gcgccggggc gcgaaggccg ccttggtctt cctgctttgc gggctgggct
      781 gcctgctttc ggtcttttat accggcctgc tggtccccac ggtcgcgggc cgttggaccc
      841 tggacaacta cctgctgccg tccaattgga tcaccgccct cctgggcggc ctgggccttc
      901 tcgccctgtg gggccccctg gggaaggcgc gggccggcgc cggagcgctg aaggccgcct
      961 ggtgcctgct ggcggcggcc ctgccccttt ggcccttgct ttcgaacttc ccgcccaacg
     1021 acgagagccg gcagatgctg cgttacgact acggcgtgaa cctgatgaaa tccacccccc
     1081 ggggcgccat cctcttctcg gagggcgacg aggatttctt cccactctat tacctccaat
     1141 tggtggaaca ccggcggccg gacctctgcg tcatcccgcc cttcaccctc ttcgagacct
     1201 ggggcgtggc cgaattggaa cgtttccacc ccgaactggg gctgaccgcc gccgggaagg
     1261 atttccccga ccatttccgc cgcatcgagt acgccctgcc cgagatcgtg gacaagaacc
     1321 gctccaaacg cgtgatcgcc ttcacctacc tgtcgggcgc cttccaccgc tattactgcc
     1381 agcccctggg ggtgaagaag gccgagtcct cgggcatcct gctgtggctc gacggccccc
     1441 tcaaccggcg gttcaaaccc ctgccccggg aggggctgcg cctgcgccac tggtgggaca
     1501 gcccctccaa cgcccacccc tccttgcggc gcgtcctctg ggcctacctg cagatgggcg
     1561 tccggccgcc cggggccccc ttgcaacccc cgtccgggcg cccgtaagat tggggtctga
     1621 ccccaatccc aaggaccccc atgcccccca aaccctcgac gctcggtgaa ttgaaggctt
     1681 ccggctatcc cgacctgacg gtcaagcagg agctggagaa gaacctcctc aagaaactga
     1741 aggccgggga gcccctcttc ccccagatcc acggtttcga ggagaccgtg gtgccccagc
     1801 tgaccaacgc catcctgtcc caccacgaca tcgtgctgtt gggcgagaaa ggccaggcca
     1861 agagccggct catgcgcgcc atcgcccagt tcctggacga ggagatcccc gtggtgcggg
     1921 gctgcgagat ccacgaccac ccctaccacc ccatctgcgc ggcctgccgc aaaaagctcg
     1981 ccgcccaggg ggacggcctg gaagtggaat ggatcggccg cgaagcccgc tacggcgagc
     2041 gcctggcgcc cggcaccaag atcgccgacc tcatcggcga cctggacccg gccaaggtgg
     2101 cggcggggaa tcccctggcc agcgaggagg cgctgcactt cggcctcatc ccccgcatga
     2161 accggggcat cttcgccgtc aacgaactgc ccgacctgga atacctggtg caggtggccc
     2221 tcttcaacat attggaggaa cgggacgtgc aggtgcgggg cttccccatc cgcttcgacc
     2281 tggacctgct gctgctcttc accgccaacc cggaggaata cacccgggcg ggcaagatca
     2341 tcacccagtt gaaggaccgc gtgggcgccg agatccgcac ccattacccg ctcacccggg
     2401 agctgggcat caagatcatc caccaggagg ccgcaccgcc gctcgatgat gggttgacgc
     2461 tcaccgtgcc cgacttcatg gtggagatca tcgagcagat caccctggag gcccggcgca
     2521 gccccttcgt gaaccagaag tcgggcgtct cggcccggct ttccatcgcc aactacgaga
     2581 ccatggtggc caacgccagg cggcgggcct acctgctggg ggaaaaggag gccgtgcccc
     2641 gcatgtcgga cctcaactac ctctacacct cctcctcggg caagatcgag ctggatccct
     2701 tccgcgagga gaccgtcacc gagttccagg tctt
//
LOCUS       DASTJJ010000159         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_147754, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000159 DASTJJ010000000
VERSION     DASTJJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147754"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFR02_01920"
     CDS             <1..217
                     /locus_tag="VFR02_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869243.1"
                     /translation="DVGEARAALERLEKVFLGAAPKVQEAFGLDLGPADFRLVYFSGP
                     LGKTLLALEDGKPVLPEAGTTPTPRPR"
     gene            complement(198..653)
                     /locus_tag="VFR02_01925"
     CDS             complement(198..653)
                     /locus_tag="VFR02_01925"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9869244.1"
                     /translation="MPLYFAYGTSLHRAQMERRMMGNKPVTRAFLRGWRLAFMGHSTE
                     RKGAVVNLLPDPGRFVPGTLYELNEVMIEYLDRCEACEKGTYAKFPVTVEKDGGEEVE
                     AMCYRLAQEAPSFGKPNLGYQMQVKQGYKEWGLPEEVLEEALAFSGGGG"
     gene            complement(656..>1150)
                     /locus_tag="VFR02_01930"
     CDS             complement(656..>1150)
                     /locus_tag="VFR02_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869245.1"
                     /translation="VSCGTVPIDPGAAGRQGLLGTQAAPAPLNPFKDYDLVMAGGEDR
                     YFKMNLPAGWYWKLFVTAVDRSNDQKGTLTAALLPSVPPWQSLPLCRDQKQFNLKPKE
                     GDQDVLGAANNGPDHPVVVELSQRGPPILVTLHSEISPLSGLLMRPLGWKRTLAPLGG
                     TGTD"
BASE COUNT          163 a          395 c          392 g          200 t
ORIGIN      
        1 ggacgtcggg gaggcccgcg ccgccctgga gcggttggaa aaggtcttct tgggcgccgc
       61 ccccaaggtc caggaggcct tcggcctgga cctgggcccg gccgacttcc ggctggtcta
      121 tttcagcggg cccctgggaa agaccctgct ggcgctggag gacgggaagc ccgtcctgcc
      181 ggaagcgggg accaccccta ccccccgccc ccgctgaagg ccagggcctc ctccagcact
      241 tcctcgggca ggccccattc cttgtagccc tgcttgacct gcatctggta gcccaggttg
      301 ggcttgccga aggacggggc ttcctgggcc aaccggtagc acatggcctc cacctcctcg
      361 ccgccgtcct tttccacggt cacggggaat ttcgcgtagg tgcccttctc gcaggcctcg
      421 cagcggtcca ggtattcgat catgacctcg ttgagctcgt acagggtgcc cggcacgaac
      481 cgccccgggt cgggcagcag gttcaccacg gcgcccttgc gttcggtgga gtggcccatg
      541 aaggccaggc gccatcccct caggaaagcg cgggtcacgg gtttgttccc catcatgcgg
      601 cgctccatct gggcccggtg caggctcgtg ccgtaggcga agtagagggg catggtcagt
      661 ccgtcccggt cccgcccagg ggggcgaggg tccttttcca tcccagcggc cgcatcagca
      721 gcccgctcag gggcgagatc tcgctgtgga gcgtcaccag gatgggcggc ccccgctggc
      781 tcaattccac caccacggga tggtcgggcc cgttgttggc cgcgcccagc acgtcctggt
      841 cgccttcctt gggcttgagg ttgaactgct tttggtcccg gcagaggggc agggactgcc
      901 agggcgggac cgacggcagc agcgccgcgg tcaatgtgcc cttttggtcg ttggatcggt
      961 cgacggcggt cacgaacagc ttccaatacc agccggccgg caggttcatc ttgaaataac
     1021 ggtcctcccc gcccgccatc accaggtcgt aatccttgaa ggggttcaag ggggcgggcg
     1081 cggcctgggt gcccaggagg ccttggcgcc cggcggcacc cgggtcgatc ggcaccgtgc
     1141 cgcaggagac
//
LOCUS       DASTJJ010000160         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1247293, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000160 DASTJJ010000000
VERSION     DASTJJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247293"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFR02_01935"
     CDS             <1..586
                     /locus_tag="VFR02_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9869246.1"
                     /translation="EPSLPDTAPDFLKLTVLTPAHREALAQLGQADSQYVSARGVFLP
                     TLSANASLSRDGWNFSGDDAAWSAGLQLSLPLFTGGRDLFDFKAAEESKQGARDRLEG
                     SDLRTESGLESAYAAYQDAVESVAVRQVQVEAAQTQEEIAKAEYLNGLLIFQNWNQIE
                     SALTNQQKQQLSDQLNVKTAEASWELAEGKGDIP"
     gene            583..1563
                     /locus_tag="VFR02_01940"
     CDS             583..1563
                     /locus_tag="VFR02_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9869247.1"
                     /translation="MRKARIIGGAACAALALLLAGGCGTKKQAENYRLVPVGRDDLRV
                     TVLATGNVQPQNQLAVLPPISGRIEKIFIKEGDHVKKGQKLMEMSSTERATLLDVAAA
                     KGPEALAHWEQLYQTTPILSPENGQVIYLPTVPGQVVGTGVTLLVMSDHLIVDTQVDE
                     TDLSQIKIGQEADIDLDAYPGRTIPAKVRRISYQSTLVNNVTNYEVDVWPDQVPAFMR
                     SGMTANVTFHVAQKADALVVPSEALKSNPDGQFYVLLAPAKAGGKPIPQVVTTGITDG
                     KETEILSGLKEGDKIALKTFSAGQYSATGSGTNPFLPSRHGSGGKSGGGH"
     gene            1848..>2293
                     /locus_tag="VFR02_01945"
     CDS             1848..>2293
                     /locus_tag="VFR02_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9869248.1"
                     /translation="MSVIEIKDIHKVYRMGDTEVRALNGVSLKIEPGEFVAIMGASGS
                     GKSTMLQILGLLDTPDKGSYKLLGREVSSLGEDELAAVRGQTLGFVFQQFNLLARTSA
                     LENVSLPLLYADPRRPHGDPAKLLQMVGLGERLGHKPNELSGGQQQR"
BASE COUNT          476 a          741 c          691 g          385 t
ORIGIN      
        1 cgaaccctcc ctgccggaca cggcgcccga tttcctgaag ctgaccgtcc ttaccccggc
       61 ccaccgggaa gccctggccc aactgggcca ggcggacagc caatatgtca gcgcccgggg
      121 cgtcttcctg cccacgctct cggccaacgc ttccctttcc cgggacggtt ggaatttctc
      181 cggggatgac gccgcctggt ccgcggggct gcaattgagc ctccccctct tcaccggcgg
      241 cagggatctt ttcgacttca aggccgccga ggaatccaag caaggcgccc gggaccgcct
      301 ggaagggtcc gacctcagga ccgaaagcgg gttggaaagc gcctacgcgg cctaccagga
      361 cgccgtggag tccgtggccg tgcgccaagt ccaggtggag gccgcccaga cccaggaaga
      421 gatcgccaag gccgagtacc tgaacgggct cttgatcttc cagaactgga accagatcga
      481 aagcgcgttg accaaccagc agaaacaaca gttgtcggac caactcaacg ttaaaaccgc
      541 cgaagccagt tgggaattgg ccgagggaaa aggagacatt ccatgaggaa agcgcggatc
      601 atcggggggg cggcctgcgc cgccctggcc ctgctgctcg cgggcggctg cgggaccaaa
      661 aaacaggcgg agaactaccg gctggtgccg gtgggaaggg acgacctgcg ggtgaccgtg
      721 ctggccacgg ggaacgtcca gccccagaac caattggcgg tgctgccacc catttcgggc
      781 cgcatcgaga agatcttcat taaagaaggc gatcacgtca aaaagggcca aaagctcatg
      841 gaaatgagtt ccaccgaacg cgccaccctg ctggacgtgg ccgccgccaa gggcccggaa
      901 gcattggccc attgggagca gctctaccag accaccccca tcctgtcgcc cgagaacggc
      961 caggtcatct acctgcccac cgtaccgggc caggtggtgg gcacgggggt gacgctcttg
     1021 gtcatgtccg accacctgat cgtggacacc caggtggacg agaccgacct gtcccagatc
     1081 aagatcggcc aggaggccga catcgacctg gacgcctatc cgggccggac catccccgcc
     1141 aaggtgcggc gcatctccta ccagtccacc ctggtgaaca acgtgaccaa ctacgaggtg
     1201 gacgtctggc ccgaccaggt gccggccttc atgcgcagcg gcatgaccgc caacgtcacc
     1261 ttccacgtgg cccagaaggc cgacgcgctg gtggtgccct ccgaggcgct caagtccaac
     1321 cccgacggcc aattctacgt gttgctggcc ccggccaagg ccggcggcaa gcccatcccc
     1381 caggtcgtca ccaccggcat cacggacggc aaggagaccg agatcctctc cgggttgaag
     1441 gaaggggata agatcgcgct caagaccttc tcggcgggcc aatattcggc gacgggctcg
     1501 ggcaccaacc ccttcctgcc ctcccggcac gggagcggcg gcaagagcgg cggcggacat
     1561 taaagacggt tcttagtttg gagttcttag tttttagagc ctctaacaaa aatcaaaata
     1621 aaggcgattt ttaccaccaa ggcaccaagt tcgccaagaa aggcgatcaa atccattcgg
     1681 attttcctgc ttttcagcct ttgcagtcct tggtgccctt ggtgtcttgg tggtgaaata
     1741 ggttttgtta gaagttctta gttgaatggc atttgaacct tccagttcaa aggccttcca
     1801 aaactaaaaa ctcaaaattg agaactcaaa acttttttgg gggtttcgtg agcgtcatcg
     1861 agatcaagga catccacaag gtctaccgca tgggcgacac cgaggtgcgc gccttgaacg
     1921 gcgtgtcgct caagatcgaa cccggcgagt tcgtggccat catgggcgcc tccgggtcgg
     1981 gcaagtcgac catgctgcag atcctggggc tgttggacac gcccgacaag ggttcctaca
     2041 agttgttggg ccgggaggtc tccagcctgg gcgaggacga gctggccgcc gtgcgcggcc
     2101 agaccctggg attcgtcttc cagcagttca acctgctggc ccgcacctcg gccctggaga
     2161 acgtgtcgct ccccctgctc tacgccgacc cccgccggcc ccacggcgac cccgccaagc
     2221 tgctccagat ggtgggcctg ggcgaacgcc tgggccacaa gcccaacgag ctttccggcg
     2281 gccagcagca gcg
//
LOCUS       DASTJJ010000161         6873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_881914, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000161 DASTJJ010000000
VERSION     DASTJJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881914"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="atpC"
                     /locus_tag="VFR02_01950"
     CDS             <1..412
                     /gene="atpC"
                     /locus_tag="VFR02_01950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET9869249.1"
                     /translation="FFKLQVVAPDHEVFHEEVSGLTAPGAGGEFGVLARHEPLLSSLK
                     PGAFTVDQGATRTVYFVSGGFIEVTPTAVTVLAEEFEPARDIDGEQEERARKKAQEKI
                     ARTKDPVERDANRAILARAQARLAAVQKAQAPSR"
     gene            416..754
                     /locus_tag="VFR02_01955"
     CDS             416..754
                     /locus_tag="VFR02_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET9869250.1"
                     /translation="MAAPDGFQAVATEAELAQNRLMVVEAGGRRLALVYSNFRAYALD
                     NACTHLGGPLGRGTLKGQTVVCPLHHWSYNFITGRVAEGVADEKVRTYEAKIEDGRVY
                     VKVPPDGSPE"
     gene            925..1305
                     /gene="acpS"
                     /locus_tag="VFR02_01960"
     CDS             925..1305
                     /gene="acpS"
                     /locus_tag="VFR02_01960"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET9869251.1"
                     /translation="MIVGTGVDLIEIERVKAAHEKHGERFLRRLFTPEEAAYCLRKSD
                     PYPSLAGRFAAKEAVIKAFSRGFGGRWKWVQIEVTREASGKPGIRLTGILEQLRRERD
                     IEAIHLSIAHSKRDAVATVILEKA"
     gene            1306..2805
                     /locus_tag="VFR02_01965"
     CDS             1306..2805
                     /locus_tag="VFR02_01965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9869252.1"
                     /translation="MARPFKPRVYTDAQLALTWFWFLAVLGLATRLSLLANNVTENTD
                     GILNLTYFSPDRVPTPRFVLLPGYPFLVWAGQRLGLDGVLWGRGLASLFGLLFLFPLW
                     KLARRWVSVEMAGMVCLMALLSPLVWQWSLRVMADTLLLLCFWACLERLIAAYLDQEE
                     KAWVAACLWGAGAALARPEGFLLLPWIAGLGWHLRGRLAVKNLLLTLLVWSLPFLFLG
                     PHLMTLLQAYQEGLGLGPGGVHPLANAAEHLYAYLTQPAFVFTPILFLTGLAGLWHMA
                     LLKSPAGRAWRRLLLPLGALLFLTRLFPTAYQDRYLMLFLPFFLVGSGYEMETLYLEW
                     RKKRGTLPALFLKNGLLAAGLAYQAFFSMVAIAYQTDSFGDLRRSGEFLKTLPAGAVI
                     YSDEVPKTHYWAGREIQPQVLPFRPRPGDYLVLHSFYTPRLGFVDRTLRDKFGAEVVY
                     ANFSSVVPLLTDLMADPAVQNRVAATGFRFQTQTFESVVYRIGRPGDIP"
     gene            2802..4355
                     /locus_tag="VFR02_01970"
     CDS             2802..4355
                     /locus_tag="VFR02_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9869253.1"
                     /translation="MKLALSSDMRRIDAAAARDWGLGTQVLMERVGEAVVRFLKRRAG
                     AAKRVGFLCGKGNNGGDALAAARRLKRRRVPVTAFLAFPPAALGPDCARQYAKARAAG
                     VPLVEAWGPEKAGTRRALEACGLLVDGLFGTGLSRKVAGAPARLIAWMNASGKPVLAL
                     DTPSGFSADRGVALGRAVRARWTLTFGLMKPGFYLPGAPAFTGEVAVADLGLPGELLA
                     SSFLTQELTDAAMVEGLLPRYGEGVHKGTRGRVLVVAGSLGLTGAAALSAWGAQRIGA
                     GLVTVACAEALNPILAVKLTECMTAPLPEGKRGYLTERSLGRLLQLAQGMDALVVGPG
                     LGRHPATGKLLAKLLPRLKAPVVLDADALYHLAARPRVLAACPAPKILTPHAGEAARL
                     LGVTASRVEAARLDIAKQLAAGYNGVALLKGPHTLIARADGALRINPTGDRGLATGGT
                     GDVLSGILGGLLAQGLGPFDAAAVGAYLHGLAGQKAGQALGPDGLMAGDLLPILPRLL
                     KAARQQDLE"
     gene            4358..5011
                     /locus_tag="VFR02_01975"
     CDS             4358..5011
                     /locus_tag="VFR02_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869254.1"
                     /translation="MPVDPALHTQWEAFLKLIQVKEAKLYDHLKKGRVKETTKYNVIL
                     ASPTPELHKEIEVGLKVYHDKITELVPTLFGANRGIKAELVSEGEWEMLAPEAKAPAV
                     PANLSSDQNELFKVLEASIRQKIEAELRSAIEAELSSGKGQEKLLARLKDDLEQEAAQ
                     HKLALRMGLTAEGDKALERLTAAWQQAKQSGDGGEPWQEVKKSLLELLDTTVQLTQS"
     gene            5037..5606
                     /locus_tag="VFR02_01980"
     CDS             5037..5606
                     /locus_tag="VFR02_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869255.1"
                     /translation="MQSYSVGPALGYLILSFFTWSATFAVVVFGSFLRRKILLWDENW
                     KETLEQGGTSIKDYADDLQRSHEKVRSIARLLGFFLVVILALMGWLVYLGVIQRPLLG
                     PAQVEQGLWLLALVSLSVVLPAYVNFSVGSYLAETMLLKANVFVYKEVREEYKEKKAK
                     IQLMEKTRELKAKREAAKAAAALEAKAAK"
     gene            5697..6053
                     /locus_tag="VFR02_01985"
     CDS             5697..6053
                     /locus_tag="VFR02_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869256.1"
                     /translation="MRKTLLFLLPALALGGCATLGVLDPSMDLKLTPQPVYRGKPALA
                     EVNAPMDAVSVVGTVDVMGSPQLIFRKDEKRGEWYFYGTIPFSPWVNPGVYKVRVLAS
                     FATGPRHYTEMEVELK"
     gene            6050..>6873
                     /locus_tag="VFR02_01990"
     CDS             6050..>6873
                     /locus_tag="VFR02_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9869257.1"
                     /translation="MSFPGLLSVVIPAYNEEGAVGQVVRRVVQVLRREKVRHEVIVVD
                     DGSRDATAARAAQAGARVVRMGENRGYGASLKAGIRQAKGDWIAILDADGTYPPEELP
                     RLLKEAPANDMVVGARTRPGAAIPALRRLPKWFLGQWANYLAERRIPDLNSGLRVFKK
                     SAMARYEGLFPNGFSFTTTITLALECHGYIVRYLPIRYLPRVGESKIRPFRDTVNFFA
                     LVLRVVMYFKPLKIFIPLSGIIFLASLASALRAHGLGQGLTAPTLLLFVAGLQIAAL"
BASE COUNT         1050 a         2352 c         2309 g         1162 t
ORIGIN      
        1 gttcttcaaa ctgcaggtgg tggcccccga tcacgaggtc ttccatgagg aggtctcggg
       61 cctcaccgcg cccggcgcgg ggggggagtt cggcgtgttg gcccgccatg agccgctcct
      121 gtccagcctc aagcccggcg ccttcacggt ggaccagggc gcgacccgga ccgtctattt
      181 cgtgtcgggg ggattcatcg aggtgacccc cacggcggtg accgtgttgg ccgaggaatt
      241 cgagcccgcc cgggacatcg acggcgaaca ggaagagcgg gcccgcaaaa aggcccagga
      301 aaagatcgcc cgcaccaagg acccggtgga gcgcgacgcc aaccgcgcca tcctggcccg
      361 ggcccaagcc cgcttggccg ccgtccaaaa ggcccaagcc ccctcccgct aggccttggc
      421 cgcccccgac gggttccaag ccgtcgccac cgaggccgaa ctcgcccaga accgcctgat
      481 ggtggtggag gccggggggc gccggctggc gctggtctat tcgaacttcc gcgcctacgc
      541 cctggacaac gcctgcaccc acctgggcgg ccccctgggc cgcggcacct tgaaggggca
      601 gaccgtggtc tgcccgctgc accactggtc ctacaacttc atcacgggcc gggtggcgga
      661 gggcgtggcc gacgagaagg tccggaccta cgaggccaag atcgaggacg gccgggtcta
      721 cgtgaaggtc ccccccgacg ggagccccga ataaaggcgg tccggaccgc ccggcggcca
      781 aggatgggat ctttccgttc tttcggggtg cccctggtgc gccgccccaa cattcagtta
      841 tattgaaaag gcgccgctct tgggggggtg gcggttcgat cggttctgtt cccaggtccc
      901 caactcatcc caaagaaggc gcccttgatc gtcggaaccg gcgtggattt gatcgagatc
      961 gagcgggtca aagcggccca cgaaaagcac ggggagcggt tcctgcgccg cctcttcacg
     1021 cccgaggaag ccgcctattg cctcaggaaa agcgacccct atccctcgct ggcggggcgc
     1081 ttcgccgcca aggaggccgt catcaaggcc ttcagccggg gtttcggcgg gcgctggaaa
     1141 tgggtccaga tcgaggtgac ccgggaggcg agcggcaagc cgggcatccg gctcaccggc
     1201 atcctggaac agctccggcg ggaacgggac atcgaggcca tccatttgtc catcgcccac
     1261 agcaaacgcg acgcggtggc caccgtgatc ctggagaaag cctgaatggc ccgccccttc
     1321 aagccccggg tctataccga cgcccaattg gccctgacct ggttctggtt cctggccgtg
     1381 ttgggcctgg ccacccgcct ttcgctgctg gccaacaacg tcaccgagaa caccgacggc
     1441 atcctgaacc tcacctattt ttcccccgac cgggtgccca cgccccgttt cgtgctgttg
     1501 cccggctacc ccttcctggt ctgggcgggg cagcgcctgg ggttggacgg ggtcctgtgg
     1561 ggcaggggct tggcgtcgct cttcggcctg ctcttccttt tcccgttgtg gaaactggcc
     1621 cgccgctggg tgtcggtgga gatggcgggc atggtctgcc tgatggccct cctgtccccg
     1681 ctggtctggc agtggtcgct cagggtgatg gccgacaccc tcctgttgct ctgtttctgg
     1741 gcctgcctgg agaggctcat cgcggcctac ctggatcagg aagagaaggc ctgggtggcc
     1801 gcctgcctct ggggcgcggg ggcggccctg gcgcggcccg agggtttcct gctgctgccc
     1861 tggatcgcgg ggctgggctg gcacctgcgg ggccggctgg ccgtgaagaa cctgctcctg
     1921 accctcttgg tctggtccct gccatttctt ttcctcggcc cccacctgat gaccctgctg
     1981 caggcctacc aggaagggct gggcctgggg cccggcggcg tccaccccct ggccaacgcc
     2041 gccgaacacc tctatgccta cctgacccag ccggccttcg tcttcacgcc catcctcttc
     2101 ctgaccgggt tggccggcct ctggcacatg gccctgctca agagcccggc gggccgcgcc
     2161 tggcggcgcc tgctgttgcc cctgggggcc ctgctgttct tgacgcgcct tttccccacg
     2221 gcctaccagg accggtacct gatgctcttc ctgcccttct tcctggtggg gtcggggtac
     2281 gagatggaaa ccctctattt ggaatggcgc aaaaaacgcg ggaccctgcc cgccctgttc
     2341 ctgaagaacg gcctgctggc ggcgggcctg gcctaccagg ccttcttttc catggtggcc
     2401 atcgcctacc agaccgattc cttcggcgac ctgcgccgat cgggcgagtt cctcaagacc
     2461 ctgccggccg gcgcggtcat ctattcggac gaggtgccca agacccatta ctgggcgggc
     2521 cgggagatcc agccccaggt gctgcccttc cggccccggc cgggggatta cctggtcctg
     2581 cacagcttct acacgccccg gttggggttc gtggaccgga ccctgcggga taagttcggc
     2641 gccgaggtcg tttacgccaa tttcagctcg gtggtcccgc tcctgaccga cctgatggcg
     2701 gaccccgcgg tgcagaaccg ggtggcggcc accggcttcc gcttccagac ccagaccttc
     2761 gagagcgtgg tctaccgcat cgggcgtccg ggggacatcc cgtgaagctc gccctgtcgt
     2821 cggacatgcg acgcatcgac gccgccgcgg cgcgcgactg gggcctggga acccaggtct
     2881 tgatggaacg cgtgggggag gccgtggtcc ggttcctcaa acgccgggcg ggcgcggcga
     2941 agcgcgtggg gttcctctgc ggcaagggca acaacggggg ggacgccctg gcggccgcgc
     3001 ggcggctcaa gcgccgacgg gtgcccgtca cggccttcct ggcctttccc ccggcggccc
     3061 tgggcccgga ctgcgcgagg cagtacgcca aggcccgcgc cgccggcgtc ccgctggtgg
     3121 aagcctgggg gccggaaaaa gcggggaccc ggcgggcctt ggaagcctgc ggcctgctgg
     3181 tggacgggct cttcggcacc ggactttcga ggaaggtcgc gggggcgccc gcccgcctga
     3241 tcgcctggat gaacgcgtcg gggaagccgg tcctggcgct ggacacgccc tcgggctttt
     3301 ccgcggaccg gggggtggcg ctgggccggg cggtccgggc ccgctggacc ctgaccttcg
     3361 ggctcatgaa gcccggcttc tacctgcccg gggcccccgc cttcacgggc gaggtggcgg
     3421 tggcggacct gggattgccc ggggaactgc tggcctcgtc cttcctcacc caagagctca
     3481 ccgacgcggc catggtggag gggctgttgc cccgttacgg ggaaggcgtc cataaaggca
     3541 cccggggccg ggtgctggtg gtggcgggct ccctggggct gaccggcgcg gcggcgcttt
     3601 cggcctgggg cgcccagcgc atcggcgcgg ggttggtgac ggtggcctgc gccgaggccc
     3661 tgaaccccat cctggccgtc aagctcaccg aatgcatgac cgcgcccctg cccgagggaa
     3721 aacgcggcta cctgaccgaa aggtccctcg gccggctgct ccaattggcc caaggcatgg
     3781 acgcgctggt ggtgggcccg ggcctgggac gccatcctgc cacggggaaa ttgctggcca
     3841 agctgctgcc ccgcctgaag gcgccggtgg tgctggacgc ggacgccctg taccacctgg
     3901 ccgcccggcc ccgggtgttg gccgcctgtc ccgcccccaa gatcctcacg ccccacgcgg
     3961 gcgaggccgc ccggcttttg ggcgtgacgg cttcccgggt ggaggccgcc cggctggaca
     4021 ttgcgaagca attggccgct ggttataatg gcgtggccct gttgaagggg ccgcacacgc
     4081 tgatcgcccg cgccgatggg gcgctccgga tcaaccccac gggggaccgg ggcctggcca
     4141 ccgggggcac gggggacgtc ctgtccggca tcctgggtgg cctcttggcc caggggctgg
     4201 gacccttcga cgccgccgcc gtgggggcct atctgcacgg cctggccgga caaaaggccg
     4261 ggcaggcgct gggacccgac ggcctgatgg ccggcgatct cttgcccatc cttccgaggc
     4321 tgctcaaagc ggcccggcaa caagatctgg agtgaccatg cccgtcgatc ccgcgctcca
     4381 cacccaatgg gaagccttcc tcaagctcat ccaggtcaag gaagccaagc tttacgacca
     4441 cctcaagaag ggccgcgtca aggaaaccac caaatataac gtgattttgg cttcccccac
     4501 ccccgagctg cacaaagaaa ttgaggtagg cctgaaagtc taccatgata agataaccga
     4561 actggtcccc accctcttcg gcgccaaccg cggcatcaag gccgaactgg tgtccgaagg
     4621 cgaatgggag atgctggccc ccgaggccaa ggccccggcg gtcccggcca acctgtcctc
     4681 cgaccagaat gagctgttca aggtgttgga ggcctccatc cgccagaaga tcgaggccga
     4741 gctgcgctcc gccatcgaag ccgagctgtc cagcggcaag ggccaggaaa agctcctggc
     4801 gcggctcaag gacgatctgg aacaggaagc ggcccagcac aagctggcgc tgcgcatggg
     4861 actgaccgcc gagggcgaca aggccctgga gcggttgacg gcggcctggc agcaggccaa
     4921 gcagagcggc gacggcgggg aaccctggca ggaagtgaag aagtccctgt tggagctttt
     4981 ggacacgacg gtgcaattga ctcaatccta attctcgcgc gttcttggga ggcggcatgc
     5041 agagctattc agtgggcccg gcgttgggtt acctcatcct ttccttcttc acctggtcgg
     5101 cgaccttcgc cgtggtggtg ttcggatcct tcctgcgccg caagatcctg ctgtgggacg
     5161 agaactggaa ggagaccctg gaacagggcg gcacttccat caaggactac gccgacgacc
     5221 tgcagcgcag ccacgagaag gtgcgctcca tcgcccggct gctgggcttc ttcctggtgg
     5281 tcatcctggc cctgatgggc tggctggtgt acttgggcgt gatccaacgg cccctgctgg
     5341 gcccggccca ggtggaacag ggcctgtggc tcttggcgct ggtgtccctg tccgtggtgc
     5401 tgcccgccta cgtgaatttc tcggtgggct cctacctggc cgagaccatg ctgctcaagg
     5461 ccaacgtctt cgtgtacaag gaagtgcgcg aggaatataa agagaagaag gccaagatcc
     5521 agctgatgga aaagacccgc gagctgaagg ccaagcggga agcggccaag gccgcggccg
     5581 ccctggaagc caaagcggcc aagtaggccg cccgccgtcc ctccagctga ccctggaccc
     5641 ttttgccgat cctccggggc cgctcccccg tcatcctgtc cctgaaaagg cccgtcatgc
     5701 gaaagaccct gctgttcctg ctcccggcct tggccctggg cggctgcgcc accctggggg
     5761 tcctggatcc gtccatggac ctcaagctca ccccccaacc cgtctaccgg ggcaaacccg
     5821 ccctggccga ggtgaacgcg cccatggacg ccgtctcggt ggtgggcacg gtggacgtga
     5881 tgggctcccc ccaactgatc ttccgcaagg acgaaaaaag gggcgagtgg tacttttacg
     5941 gcaccatccc cttctcgccc tgggtgaacc cgggcgtcta taaggtccgg gtgttggcca
     6001 gcttcgcgac gggcccccgc cactacaccg agatggaagt ggagctcaaa tgagctttcc
     6061 cggcctgctg tcggtggtca tccccgccta caacgaggaa ggggcggtgg gccaggtggt
     6121 gcgccgggtc gtccaggtcc ttcgccggga aaaggtgcgc catgaggtga tcgtggtgga
     6181 cgacggcagc cgggacgcca ccgcggcccg ggccgcccag gccggggccc gggtggtgcg
     6241 catgggggag aaccgcggct acggcgcttc cctgaaggcg ggcatccggc aggccaaggg
     6301 cgattggatc gccatcctgg acgccgacgg cacctacccg cccgaggaac tgccccggct
     6361 gctgaaggaa gccccggcca acgacatggt ggtgggggcc cgcacccggc ccggggccgc
     6421 catcccggcg ctgcgccgcc tgcccaaatg gttcttgggc cagtgggcca actacctggc
     6481 cgagcgccgc atccccgacc tcaattcggg cctgcgggtc ttcaagaaga gcgccatggc
     6541 ccgctatgag ggccttttcc ccaacgggtt ttccttcacc accaccatca cgctggcgct
     6601 ggaatgccac ggctacatcg tgcggtacct gcccatccgt tacctgccgc gggtggggga
     6661 gtccaagatc aggcccttcc gggacaccgt caattttttt gccttggtct tgagggtggt
     6721 catgtatttc aaacccttga agatttttat ccccctgagc ggtatcatat ttttagcctc
     6781 cctggcgtcc gctttgcggg cccacgggct gggccagggc ctgaccgccc ccaccctctt
     6841 actctttgtc gccggcttgc agatcgccgc cct
//
LOCUS       DASTJJ010000162         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_147901, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000162 DASTJJ010000000
VERSION     DASTJJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147901"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFR02_01995"
     CDS             complement(<1..1063)
                     /locus_tag="VFR02_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869258.1"
                     /translation="MRLPLILALFLAAASWPARAAQTVYPAAGWANPLDGENPFARAQ
                     ALGSAFAGVADDGSALYSNPAGLAYLESPQVLSGTGLWLVDSFEQDLLAAFPLAPGAG
                     AAVAGRYLGFGSLPGYDASGTATGSYGADRLGLSAGAGIELWPGWAAGLQAQMDRDRL
                     DQTLLTDFTWGLGVLGRLSPRLSLGASFHHAGWGASGQGLPGILEIGAAWRADLGARQ
                     SLLAALGGTWEAGQDGFVQGGLEYGWARVFFLRLGARRAFQDQGTGIGLTAGAGADLG
                     DLQLDYAYAPYGDLGSSHRFTVGYRFPPARPAPTPTAVVWPVPPDLPPVPPETENQPT
                     PVPAGDRLSVAPGQAVDPLT"
     gene            complement(1147..1806)
                     /locus_tag="VFR02_02000"
     CDS             complement(1147..1806)
                     /locus_tag="VFR02_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9869259.1"
                     /translation="MADDLIQKFFRDDLTEAEEKALSERLQGSTEDALRFGEEAEKRY
                     QAYGLPDPEPPRPLARPMPEPPVHFLWLHAVLKALAVLLGLAVAGVVVLGVWRAFVPP
                     DDKAAPPLPAASSPGLSDEAVVLQPSRPAAAARRTVPTATPDLSITVHKASTGPVTVQ
                     VLDMDGQTLRSLYQGILRQGDLSFEWDGKLADGSPAPSGLYRIRLESGGAPQEKIIRI
                     P"
     gene            complement(1796..2371)
                     /locus_tag="VFR02_02005"
     CDS             complement(1796..2371)
                     /locus_tag="VFR02_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9869260.1"
                     /translation="MEDRELVGRLLAKDEEAFRYFYDAYQTKIYKACSYLLGYQDPDA
                     EDLAQEVFLAALKQLPRFEFRSSLYHWLYRIAMYLCYERVRRRRRMVASEEEALETAA
                     LSGARDRQDKAEEENEKKRMTEILTRQKELLGEPCRGLLDLRDGQSLSYARIADVLRV
                     PIGTVMSRLARCRESLKALFLKALREGPHGR"
     gene            complement(2554..3795)
                     /gene="icd"
                     /locus_tag="VFR02_02010"
     CDS             complement(2554..3795)
                     /gene="icd"
                     /locus_tag="VFR02_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET9869261.1"
                     /translation="MAYAYQSVKPPQGDKVHIEKGLLKVPDHPVIPFIEGDGTGPDIW
                     KASVRIFDAAVAKAYGGKKKISWFEVYAGQTAFDKFQNWLPDDTVEAFKEYLVGIKGP
                     LTTPVGKGIRSLNVALRQMLDLYVCLRPVRYFTGVPSPVKHPEKVDMVIFRENTEDIY
                     AGIEWAADSPEAKKVIAWLQQEMGVKKIRFPETSGIGIKPVSQEGTERLVRAAIQYAI
                     DHQRKSVTLVHKGNIMKFTEGAFRDWGYALAKKEFGAVEIDGGPWCKTKQGIVVKDAI
                     ADITLQQVLTRPEDFDVIATLNLNGDYLSDALAAQVGGIGIAPGGNINYVTGHAIFEA
                     THGTAPKYANQDKVNPGSVVLSGVMMLEHLGWQEAADSIVKALEKTIDQKTVTYDFAR
                     LMQGAKEVKCSEFADKVIANI"
     gene            complement(4051..>4297)
                     /locus_tag="VFR02_02015"
     CDS             complement(4051..>4297)
                     /locus_tag="VFR02_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9869262.1"
                     /translation="CLLATGAEIAELDLPVCAPSAERLGDLLEVTEKAYLVDALTKAQ
                     GNLLTTARQAGLGLKTLQRKMRKYGLNGKDFRDMTGV"
BASE COUNT          690 a         1430 c         1455 g          722 t
ORIGIN      
        1 cggtcagggg atccaccgcc tgcccgggcg ccacgctcag gcggtcaccc gccgggaccg
       61 gggtgggctg gttttcagtt tccggcggca ccgggggcag gtcgggcgga acgggccaga
      121 ccacggccgt cggcgtcggg gcgggccggg ccggggggaa tcggtagccc accgtgaagc
      181 ggtgggaaga tcccaggtcc ccataaggcg cgtaggcgta gtccaattga aggtccccca
      241 ggtcggctcc ggcccccgcc gttagcccga tgcccgtgcc ctggtcctgg aaagcccggc
      301 gcgcgcccag ccgcaggaaa aagacccgcg cccaaccgta ttccagcccc ccctggacaa
      361 aaccgtcctg gcccgcctcc caggtcccgc ccagcgccgc caaaagcgat tgccgggccc
      421 ccaggtccgc ccgccaagcc gcgccgatct ccaggatgcc gggcagtccc tgccccgagg
      481 ccccccaacc ggcgtggtgg aaggacgcgc ccaggctcaa tcgcggggaa agccggccca
      541 ggacccccaa gccccaggtg aagtcggtaa ggagggtctg gtccaaccgg tcccggtcca
      601 tctgggcctg gagccccgcc gcccagccgg gccaaagctc gatcccggcc cccgcgctca
      661 agcccaaccg gtcggcgccg taggatcccg tggcggtgcc cgacgcgtcg tagcccggga
      721 gcgacccgaa gcctaaataa cgtcccgcca ccgcggcgcc cgcgcccggg gccaggggga
      781 aagccgccaa gaggtcctgc tcgaagctgt ccaccagcca gaggccggtg ccggaaagga
      841 cctgggggga ctcgaggtac gccaggcccg ccgggttcga atagagcgcg gagccgtcgt
      901 ccgcgacgcc cgcgaaagcg gatccaagcg cctgggcgcg ggcgaagggg ttttccccgt
      961 ccagcgggtt ggcccatccc gccgccggat agaccgtctg cgccgcgcgc gcgggccagg
     1021 aggccgcagc gaggaacagg gcgaggatca agggcaggcg catccgcatt tccttggggc
     1081 cggggaaggc cgtcccccgg gcgcgtccat tttaacaggg cgatcgggga accgcccccc
     1141 tcggggtcag gggatccgga tgatcttttc ctggggcgcc ccgccggatt ccagccggat
     1201 ccggtacagg cccgaaggcg cgggcgatcc gtccgccagt tttccgtccc actcaaagct
     1261 caggtccccc tggcgcagga tgccctgata gagggaccgc agggtttggc cgtccatatc
     1321 cagcacctgc acggtcaccg ggcccgtcga cgccttatgc accgtgatgg aaaggtcggg
     1381 ggtcgccgtc gggacggtcc ggcgcgcggc cgcggccggg cgggaaggct gcagcaccac
     1441 ggcttcgtcg gacaggccgg ggcttgaggc cgcgggcagg ggcggggcgg ccttgtcgtc
     1501 cggcggcacg aaggcgcgcc agacccccag gaccaccacg cccgccacgg ccaaccccag
     1561 caggacggcc aaggccttca ggaccgcgtg gagccagagg aaatggacgg gcggctcggg
     1621 catggggcgg gccaggggcc gcgggggctc cgggtcgggc aagccgtagg cctggtagcg
     1681 tttctccgct tcctccccga aacgcagggc gtcctcggtg gacccttgga ggcgctcgga
     1741 cagggccttt tcctcggcct cggtcaggtc gtcccggaag aatttttgga tgaggtcatc
     1801 ggccatgggg tccctcccgc agggccttga ggaaaagggc cttcaaggat tcccggcagc
     1861 gcgccaaccg ggacatgacc gtgccgatgg gcacgcgcag gacgtccgcg atccgggcgt
     1921 agctcaggct ttggccgtcg cgcaggtcca acaggccccg gcagggctcg cccagcagtt
     1981 ctttttgccg cgtcaggatc tcggtcatcc gttttttctc gttctcttcc tcggccttat
     2041 cttgccggtc ccgcgcgccg gacagggccg ccgtctccag ggcctcttcc tcgctggcca
     2101 ccatccgccg ccggcgccgc acccgctcgt aacagaggta catggcgatg cggtagagcc
     2161 agtgatagag gctggaacgg aactcgaacc ggggcagttg cttcagcgcc gccaggaaca
     2221 cctcctgggc caggtcctcg gcgtccgggt cctggtagcc caacaggtag gagcaggctt
     2281 tgtagatctt ggtctggtag gcatcgtaaa aataccggaa ggcttcttca tccttcgcca
     2341 ataaacggcc caccaattca cggtcttcca atagaggtcc ccatcaagac ccgcccgctc
     2401 ctgatagacc agtgggcggg gaggcggttt tattccctgg aatttaagcg aaaaaggggc
     2461 ccgccacaac cttttcgtcg ggatcttggg ggggggtgaa cgaaaaaagc ccgtcccacg
     2521 cttggcgggg acgggcttgg gggaccggac cggctagatg ttcgcgatga ccttgtcggc
     2581 gaactccgag cacttcactt ccttcgcgcc ctgcatgagc cgcgcgaagt cgtaggtgac
     2641 ggtcttttgg tcgatggtct tttcgagcgc cttcacgatg ctgtcggcgg cctcctgcca
     2701 gcccaggtgc tccagcatca tgacgccgga cagcaccacc gaccccggat tcaccttgtc
     2761 ctggttggcg tacttggggg ccgtgccgtg ggtggcctcg aagatggcgt ggcccgtcac
     2821 gtagttgatg ttgcccccgg gcgcgatgcc gatgccgccc acctgggcgg ccagcgcgtc
     2881 cgacaggtag tcgccgttga ggttgagggt ggcgatgacg tcgaaatcct cgggccgggt
     2941 cagcacctgc tgcagggtga tgtcggcgat ggcgtccttg acgacgatgc cctgcttcgt
     3001 tttgcaccag ggcccgccgt cgatctcgac cgcgccgaac tccttcttgg ccagggcgta
     3061 gccccagtcc cggaaggcgc cctcggtgaa cttcatgatg ttgcccttgt gcaccagggt
     3121 gacgctcttg cgctggtggt cgatggcgta ctgaatggcc gcgcgcacca ggcgttcggt
     3181 gccttcctgg gacacgggct tgatgccgat gcccgaggtc tcggggaaac ggatcttctt
     3241 gacgcccatc tcctgctgca gccacgcgat gaccttcttg gcctcggggc tgtcggcggc
     3301 ccattcgatg cccgcgtaga tgtcctcggt gttctcgcgg aagatgacca tgtccacctt
     3361 ctcgggatgc ttgaccggcg agggcacgcc cgtgaaatag cgcacgggcc tcaggcacac
     3421 gtagaggtcc agcatctgcc gcagggccac gttcagcgac cggatgccct tgcccaccgg
     3481 cgtggtcagg gggcccttga tgcccaccag gtattccttg aaggcctcca cggtgtcgtc
     3541 gggcagccag ttctggaact tgtcgaaggc ggtctggccc gcgtagacct cgaaccagga
     3601 gatcttcttc ttgccgccgt aggccttggc caccgccgcg tcgaaaatgc gcacggacgc
     3661 cttccagatg tccggccccg tgccgtcgcc ctcgatgaag gggatgaccg ggtggtcggg
     3721 caccttcaac aggcccttct cgatatggac cttgtcgcct tggggcggtt tcacggactg
     3781 gtaggcgtaa gccacggtgg actccttggg agggttggga aaacgcgggc agtttatcaa
     3841 aaggggcggc ccggggccac ccttagaact tcaaacaaaa acctgttcca ccacatatgg
     3901 ccgagggcgg cctcgttcca aagatccaat gatggggcag gcgccaaaca ccgcaaagac
     3961 agaagatccg agaaattcga tgaactcgat cgcctttctt cgtgccttgg tggtgaaaat
     4021 tgctttcatt ttgatttttg ttagcggctc ttagactccg gtcatatcgc ggaagtcctt
     4081 gccgttgagg ccgtacttgc gcatcttgcg ctgcagggtc ttgaggccca gccccgcctg
     4141 gcgggcggtg gtgagcaggt tgccctgggc cttggtgagg gcgtccacca ggtaggcctt
     4201 ctcggtgacc tccagcaggt cgcccaggcg ctcggcggaa ggcgcgcaga ccggcaggtc
     4261 cagctcggcg atctcggcgc cggtcgccag caggcag
//
LOCUS       DASTJJ010000163         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_636388, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000163 DASTJJ010000000
VERSION     DASTJJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636388"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..918
                     /locus_tag="VFR02_02020"
     CDS             367..918
                     /locus_tag="VFR02_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9869263.1"
                     /translation="MDERELVQKILAGDAEAKGLFFTTHRERVYRNCVHLLGYQDPEA
                     EDVAQEVFLTAFEKLPGFEFRSTLATWLTQICIYKCHNRFRQRAKLVQQEEAGLEELL
                     RPAALARDDREGGLEKWRVVEKCLAQMGKDCREIIELRELKTLSYVEVGKALKIPLGT
                     VMSRLSRCKRALKTLALQFLKDG"
     gene            920..1552
                     /locus_tag="VFR02_02025"
     CDS             920..1552
                     /locus_tag="VFR02_02025"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9869264.1"
                     /translation="MADLVEEFFKRDLTEAEHEALAGLLGSSPDAALRYEGLLAENYL
                     ATGLPSPTLPRGLESLPGPGALGNVAWLKIALVAAGLTAAGLALWKFWPKPVLEVPAP
                     GLQAPARPHPARPQAALPGREGEELSVMVKAPEKSLVSVRVLDGAGKEVRSLYTGFVE
                     AGQWAFQWDGLLQDGRPAQAGNYLIDVRSGTAHLTKGIRIKVQPAISHGP"
     gene            1622..>1891
                     /locus_tag="VFR02_02030"
     CDS             1622..>1891
                     /locus_tag="VFR02_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HET9869265.1"
                     /translation="MKPILPLLFILSLLAPAGRALAQFDGDGLKAVYYTNPDLTSAAV
                     SVTDPQVSFRWNGCPPQPGMSASSFSVRWTGWLEAAYSEPYTIITV"
BASE COUNT          302 a          645 c          615 g          329 t
ORIGIN      
        1 cgttgttggg catcgggctc ttcgcccaat acaaaggttg gggtatgctc tgggccttca
       61 tcccggccct cctgctcggc ttcgtggctt atggaaggat ggccttgagg cccttctaag
      121 gccatcccgg tcctgaaagg acctccaatg cccgccgccg acccctcctt tcccggcccc
      181 agggccctgc gcgccgccct ttgacggccg ttgcgtgtcg cggtcgccgc ggcgccgcgg
      241 gtgccccact cacccggttt ttcccccttt ccggctcccc cgttgccgcc cccttgccgc
      301 atgctatact ccccaattgg ggaataaagc cttcccgtta aaacactaat gaatggcggg
      361 gtatccttgg acgagaggga actggttcaa aagatcctgg cgggcgacgc ggaggccaag
      421 ggccttttct tcaccaccca ccgggaacgg gtctaccgca attgcgtcca cctgctcggg
      481 taccaagacc cggaggccga ggacgtggcg caagaggtgt tcctgaccgc cttcgagaaa
      541 ctgccgggct tcgagttccg gtcgaccctg gcgacctggc tgacccagat ctgcatctac
      601 aagtgccaca accggttccg ccagcgcgcc aagctggtgc agcaggagga agccggcctg
      661 gaggagctgc tgaggcccgc ggccctggcc cgggacgacc gggagggcgg cctggagaaa
      721 tggcgcgtgg tcgagaagtg cctggcccaa atgggcaagg attgccggga gatcattgag
      781 cttcgggaac tgaagaccct gagctacgtg gaagtgggca aggccctgaa gatccccctg
      841 gggacggtga tgtcccggct ttcccggtgc aagcgggccc tgaagacgct ggctttacag
      901 ttcttgaagg acggttgaca tggcggacct ggtggaagaa ttcttcaaac gcgacctgac
      961 cgaggccgag cacgaggcct tggccgggct tttgggttcg tcccccgacg cggccctgcg
     1021 ctatgagggg ctcctggccg agaactacct ggccacgggt ctgccgtcgc ccaccctgcc
     1081 ccgggggctt gagtccctgc cgggccccgg cgccctgggc aacgtcgcct ggctcaagat
     1141 cgccctcgtg gcggcgggcc tgacggcggc gggcctggcc ctttggaaat tctggcccaa
     1201 gcccgtgctg gaggtcccgg ctcccggcct gcaagcgccc gcccgtcccc atcccgcgcg
     1261 gccccaagcg gccctgcccg gccgggaagg cgaggaactg agcgtgatgg tgaaggcccc
     1321 cgaaaagtcc ctggtgagcg tgcgcgtgct ggacggggcg gggaaggaag tgcggtcgct
     1381 ctacacgggc ttcgtggagg ccggccaatg ggccttccag tgggacgggc tgcttcagga
     1441 cggccggcca gcccaggccg ggaattacct catcgacgtg cgctccggga ccgcccacct
     1501 gaccaagggc atccggatca aggtccaacc ggccatttcc cacgggcctt aagaatcctg
     1561 tggcgcccgg cgcggcgggg gggaataatc gcttatcgga ccaccgaacg gcgagggacc
     1621 cttgaagccg atcctgcccc ttcttttcat cctttccctc ctggcccccg cgggccgggc
     1681 cctggcccaa ttcgacgggg acggcctgaa ggccgtctat tacaccaatc ccgacctcac
     1741 cagcgcggcc gtcagcgtga ccgatcccca ggtcagtttc cgctggaacg gctgcccgcc
     1801 ccagcccggc atgtccgcca gttccttctc ggtgcgttgg acgggctggt tggaagccgc
     1861 ctatagcgaa ccctatacca tcatcacggt g
//
LOCUS       DASTJJ010000164         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_636448, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000164 DASTJJ010000000
VERSION     DASTJJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636448"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..319
                     /gene="acpP"
                     /locus_tag="VFR02_02035"
     CDS             86..319
                     /gene="acpP"
                     /locus_tag="VFR02_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9869266.1"
                     /translation="MAIEDKVKEIIVDQLGVEADQVKPEASFIDDLGADSLDTVELVM
                     AFEEEFDLEIPDEDAEKIKTVGNAIDYIKSKAS"
     gene            356..1591
                     /gene="fabF"
                     /locus_tag="VFR02_02040"
     CDS             356..1591
                     /gene="fabF"
                     /locus_tag="VFR02_02040"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9869267.1"
                     /translation="MVVTGLGVVAPTGNNPVDFFRNICDGKSGIGLVTRFDASRVASK
                     IGGEVKDFDPVSFGIEKKDLKKTDLFVQFALAASLQAAKDAGLDMAKEDSARVGVLIG
                     SGMGGLSTLEKQHARLVQDGPDRVSPFLIPMMIINMASGMVSIYLGARGPNVSTVSAC
                     ASGAHAIGEAMRWIQKGDADVVIAGGAEACITELGYAGFCNMTALCMDSNEAPAKGSR
                     PFDKARSGFVMGEGAGIVVLESLEHAKARGAKIYAEALGYGMTADAYHMTTPAENGEG
                     AARSMQLALDDAKLKPADIGYINAHGTSTPYNDKFETMAIKTVFKDQAAKVPVSSTKS
                     TTGHLLGAAGGVEFVACVMAIQEKVLPPTINQETPDPDCDLDYIPNVKREASVEVCLS
                     NSLGFGGHNVSLIVRKFNP"
     gene            1569..>2071
                     /locus_tag="VFR02_02045"
     CDS             1569..>2071
                     /locus_tag="VFR02_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HET9869268.1"
                     /translation="MFENLIPKKKGRAPAHLPVGEALEPERLALLRRFAKEQRFPLGD
                     GRLLNDALTHKSYAHERKLKAHFHNEKLEFLGDSVLGLVISEHVYRSYPEANEGGLSK
                     VKSVVVSATVLGEKARLMGLGDYLLLGKGEEKSGGKNRVSLLADALEAVFGAVYLESG
                     LPSARKL"
BASE COUNT          398 a          645 c          681 g          347 t
ORIGIN      
        1 agtcctttgc gtggacgggg gaatggtgat ataagggttt gtttcgcggg gggccccggg
       61 cccccctaat ttacggaggt ggagaatggc gatcgaagac aaggtgaagg aaatcattgt
      121 ggatcagttg ggcgtggaag cggaccaagt gaagcccgag gcctctttca tcgacgatct
      181 gggcgcggat tccctggata cggtggagct ggtcatggcc ttcgaagaag aattcgacct
      241 ggagatcccc gatgaggacg ccgagaagat caagaccgtc ggcaacgcca tcgactacat
      301 caagtcgaaa gcctcgtaag acccagtccc gattttggag gaatccttga aacgagtggt
      361 ggtcaccggt ttgggcgtgg ttgcgcccac aggcaacaac cctgtcgatt ttttcaggaa
      421 catctgcgac ggcaagagcg gcatcggcct ggtgacccgc ttcgacgcct cgcgggtggc
      481 cagtaagatc ggcggcgagg tcaaggattt cgacccggtg tcctttggga tcgagaagaa
      541 ggacctgaag aagaccgatc tgttcgtcca attcgccctg gcggccagcc tccaggccgc
      601 gaaggacgcg gggttggaca tggccaagga ggactccgcc cgggtgggcg tcctcatcgg
      661 ttctggcatg ggcggcctct ccacgctgga aaagcagcac gcccgcctgg tgcaggacgg
      721 cccggaccgc gtcagcccct tcctgatccc catgatgatc atcaacatgg cctcgggcat
      781 ggtgtccatt tatttgggcg cccggggccc caacgtgtcc accgtctcgg cctgcgcgtc
      841 gggggcccac gccatcggcg aggccatgcg ctggatccag aagggcgacg ccgacgtcgt
      901 catcgcgggg ggggcggagg cctgcatcac cgagctcggg tacgccgggt tctgcaacat
      961 gacggccctt tgcatggatt ccaacgaggc ccccgccaag gggagccgtc ccttcgacaa
     1021 ggcccgctcg ggcttcgtca tgggcgaggg cgcgggcatc gtggtgttgg agtcgctgga
     1081 gcacgcgaag gcccggggcg ccaagatcta cgccgaggcc ctgggatacg gcatgacggc
     1141 cgacgcctac cacatgacca ccccggcgga gaacggcgag ggcgcggccc ggtccatgca
     1201 gttggccctg gacgacgcca agctcaagcc cgccgacatc ggctacatca acgcccacgg
     1261 cacctccacg ccttacaacg acaagttcga gaccatggcc atcaagacgg tcttcaagga
     1321 ccaggcggcc aaggtccccg tttcctcgac caaatccacc accgggcacc tgttgggcgc
     1381 cgcgggcggc gtggagttcg tggcctgcgt gatggccatc caggaaaagg tgctgccgcc
     1441 caccatcaac caagagaccc cggacccgga ttgcgacctg gattacatcc ccaacgtcaa
     1501 gcgggaggcc tccgtggaag tctgcctgtc caactcgctg ggattcggcg gccacaatgt
     1561 ctccctcatt gttcgaaaat ttaatcccta agaaaaaggg ccgcgccccg gcccacctgc
     1621 cggtggggga ggcgctggaa ccggagcgcc tggccctgtt gcgccgtttc gccaaggaac
     1681 agcgtttccc cctgggggac gggcggctgc tcaacgacgc cctcacccac aagtcctacg
     1741 cccacgaacg caagctcaag gcccatttcc acaacgagaa gctggagttc ttgggcgact
     1801 cggtgctggg attggtcatc agcgagcacg tctaccggtc ctatccggaa gccaacgagg
     1861 gcggcctttc caaggtcaag agcgtggtgg tgagcgcgac cgtcctgggg gagaaggccc
     1921 ggctcatggg cctgggggat tacctgctcc tgggcaaggg cgaggaaaag tcgggcggca
     1981 agaaccgcgt ctcgctgttg gccgacgccc tggaggccgt cttcggcgcc gtgtacctgg
     2041 aaagcggcct gccctcggcc cgcaagctca t
//
LOCUS       DASTJJ010000165         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_393045, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000165 DASTJJ010000000
VERSION     DASTJJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393045"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1700
                     /locus_tag="VFR02_02050"
     CDS             102..>1700
                     /locus_tag="VFR02_02050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9869269.1"
                     /translation="MASAKKKNKPGPTNPVTGPWGSAMLASIFIGGQILCLKGFAFPG
                     MALSLLAAITWLAAYFGVFHPQFDRKAVPVPKPARNARPGRAAAAPALKPVFTAVDFY
                     RLLAVLAGLMLAGMGQSYWIQMSRPATLLIGAFYYLGAVVLWLGAMGPWFKEGPAESE
                     PLLTREKAVFWAILAVAFFFRVWHLDSFPKGLFIDQGYEGYSALRILHEGWHPFYVED
                     IYHNYALVLYQIAAFFAVVGASDFTLKLFFVLMVMATFPLIYWTFRQLAGPRIALLSL
                     FVLAVMRWNVNFSRNAFPTVQVPFYMFGTLAFLIYGLRQGKRWAFVVASLFFTAGFYT
                     YQAFKVFPLLLVPYAAYEAVVHWKSLKANAKNVLLFLGLAIALTGPVWYDMVATHNLG
                     TRESELSIFPQVRAAHSWAPFFNVLDKSALMFNRQGDPNPRHNLQDHRMLDDVSGVLL
                     ILGLFYSLVRIRRPGYFYAVVGFFVMSVPGVFSIDPAHANRLLGMTPFIAFLIAAPLS
                     ALWGQAQAWAGKAGERAFLVLLALA"
BASE COUNT          229 a          587 c          511 g          373 t
ORIGIN      
        1 caagctggga ttgtccacgc ttttgaagta tctgggccgc gccctggccc tgcggttcga
       61 gggcttccgc ccggccgcct gacaccatcc aaggagcatc catggcttcc gcgaagaaaa
      121 agaacaagcc gggcccgacc aatcccgtca ccgggccctg gggctcggcg atgctggcct
      181 cgatcttcat cggcggtcag atcctatgtt tgaaggggtt cgccttcccg ggcatggcct
      241 tgtccctgct ggcggccatc acctggttgg cggcctattt cggcgtcttc cacccgcaat
      301 tcgaccggaa agccgtcccc gtgcccaagc ctgcccggaa cgcccgtccg ggccgggcgg
      361 cggccgcccc cgccttgaag ccggtcttca cggcggtcga tttttaccgc ctgttggccg
      421 tgctggccgg gctcatgctg gccggcatgg gccagtccta ctggatccag atgtcccgcc
      481 ccgccacgct gttgatcggc gccttctatt atttgggcgc ggtggtcctc tggttggggg
      541 ccatggggcc ctggttcaag gaaggcccgg ccgaatcgga gcccctcttg acccgtgaaa
      601 aggcggtctt ctgggccatc ctggcggtgg ccttcttctt ccgcgtttgg cacctggaca
      661 gtttccccaa gggcctcttc atcgaccagg gctacgaggg ttattcggcc ctgcgcatcc
      721 tgcatgaggg ctggcatccc ttttatgtgg aggacatcta ccacaactac gccctggtgc
      781 tgtaccagat cgcggccttt ttcgccgtgg tgggcgcctc ggacttcacc ttgaagctct
      841 tcttcgtgct gatggtgatg gccacctttc cgctcatcta ctggaccttc cgccaactgg
      901 cggggccgcg catcgccctg ctgtcgctct tcgtgctggc ggtcatgcgc tggaacgtca
      961 atttcagccg caacgccttc cccacggtgc aggtgccctt ctatatgttc ggcaccctgg
     1021 ccttcctgat ctacggcctc cggcagggca agcgctgggc cttcgtggtg gcctcgctct
     1081 tcttcacggc gggtttctac acctaccagg ccttcaaggt cttccccctg ctgttggtgc
     1141 cctacgcggc ctatgaggcc gtggtccatt ggaagtccct caaggccaac gccaagaacg
     1201 tcctattgtt cctggggctg gccatcgccc tgaccggacc ggtctggtac gacatggtgg
     1261 ccacccacaa cctgggtacg cgggaatcgg aactttccat cttcccccag gtgcgcgccg
     1321 cccacagttg ggcgcccttc ttcaacgtgc tggacaagag cgccctcatg ttcaaccgcc
     1381 agggcgaccc caacccgcgc cataacctgc aggaccaccg catgctggac gacgtctcgg
     1441 gcgtgctgct catcctgggc cttttctaca gcttggtgcg gatccggcgg ccgggttatt
     1501 tttacgccgt ggtggggttc ttcgtcatga gcgtgcccgg ggtcttttcc atcgacccgg
     1561 cccacgccaa ccgcctgctg ggcatgacgc ccttcatcgc cttcctgatc gccgcgcccc
     1621 tgtccgcgct ctgggggcag gcccaggcct gggcgggcaa ggcgggggaa agggccttcc
     1681 tggttctgct ggccctggcg
//
LOCUS       DASTJJ010000166         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_882284, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000166 DASTJJ010000000
VERSION     DASTJJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882284"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFR02_02055"
     CDS             complement(<1..405)
                     /locus_tag="VFR02_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869270.1"
                     /translation="MGKTWIAAMAAALCLVGCTHPAGKTDPQLRAIEDSITAGNYDAA
                     VSQTRAVEAQSPAPSCAPQALYLEGYAMAYGWGDYRRARKPLEQLLDAAPAEPLASKA
                     LRLLADCDYWQGYYARAIREYQKLSSSPDPETR"
     gene            complement(417..>1222)
                     /locus_tag="VFR02_02060"
     CDS             complement(417..>1222)
                     /locus_tag="VFR02_02060"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9869271.1"
                     /translation="ALWIKTSEFGSPKDEVLVKANGAPAYFAADIAYHLGKFDRGFDR
                     ILDIWGPDHHGHIQRMKSALQALGRPVERFSVLIAQQVNLLQDGEKVKMSKREGKFIT
                     MDELLEQVGKDAARFFFVMRSANSHLDFDIAVAQKQTDENPVYYIQYAHARICSLLKK
                     AEAEKGFRPSGDAGELAGLSAPEETALLREILEYPQWVEESGRSYEPHRIVAYLQNLS
                     AQFHVFYARHRILDSAPALGNARLALALGVKNVIRNALELLGVSAPESM"
BASE COUNT          193 a          413 c          399 g          217 t
ORIGIN      
        1 ccgggtctcg ggatcggggg acgatgaaag tttctggtat tcgcggatgg cccgggcgta
       61 atagccctgc cagtagtcgc aatccgccag gagccgcagg gccttggaag ccaggggctc
      121 ggcgggcgcc gcgtccaaga gctgttccag gggcttgcgg gcccgccggt aatcgcccca
      181 gccgtaggcc atggcgtagc cctccaggta aagggcctgg ggcgcgcagg aaggcgccgg
      241 cgattgggcc tcgaccgccc gcgtctggct gaccgcggcg tcgtaattgc ccgcggtgat
      301 cgagtcctcg atcgccctca actggggatc cgtcttgccg gccggatggg tgcaccccac
      361 caggcaaagt gcggcggcca tggccgcgat ccatgttttt cccaccccga cctccctcac
      421 atcgactcgg gggccgagac ccccaacaac tccagcgcgt tgcggatgac gttcttcacg
      481 cccagggcca gcgccaaccg ggcgttgccc agggccggcg ccgaatccag gatccggtgg
      541 cgggcgtaga agacgtggaa ctgcgccgac aggttctgca ggtaggccac gatccggtgc
      601 ggttcgtagc tgcggccgct ctcctccacc cattggggat attccaatat ctcccgcaac
      661 agggccgtct cctcgggcgc cgacagcccg gccaattccc ccgcgtcccc cgaggggcgg
      721 aaccccttct cggcctccgc cttcttgagc aggctgcaga tgcgcgcgtg ggcgtactgg
      781 atgtaataga ccgggttctc gtcggtctgc ttttgggcca ccgcgatgtc gaaatccagg
      841 tggctgttgg cgctgcgcat gacgaaaaag aaccgggcgg cgtccttccc cacctgttcc
      901 aagagctcgt ccatggtgat gaacttgccc tcgcgtttgg acatcttcac cttttccccg
      961 tcctgcagca ggttcacctg ctgggcgatg agcacgctga aacgctccac gggccggccc
     1021 agggcctgca gggcggactt catgcgctgg atgtggccat ggtggtccgg cccccagatg
     1081 tccaggatgc ggtcgaaccc ccggtcgaac ttgcccaggt gataggcgat gtccgccgcg
     1141 aaataggccg gcgccccgtt ggccttgacc aacacctcgt ccttggggga cccgaactcg
     1201 gaggtcttga tccaaagggc cc
//
LOCUS       DASTJJ010000167         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1494199, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000167 DASTJJ010000000
VERSION     DASTJJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494199"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFR02_02065"
     CDS             <1..588
                     /locus_tag="VFR02_02065"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET9869272.1"
                     /translation="EKNIGFMDVGVSGGVWGLKNGYGLMAGGSAQHVEWALPILDALR
                     PEGDRADSFVHVGDVGAGHYAKMVHNGIEYGLMQAYAEGYELLSKKEIIKDLPGTFRA
                     WQKGTVVRSWLLDLMVKALDEDPGLETIDDYVEDSGEGRWTVEEAIANAVPAPAITAA
                     LFARFSSREDNSPAMKMVSALRHQFGGHATKPSGS"
     gene            612..>1194
                     /gene="recF"
                     /locus_tag="VFR02_02070"
     CDS             612..>1194
                     /gene="recF"
                     /locus_tag="VFR02_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920278.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET9869273.1"
                     /translation="MYLEHLSLTDFRSYAQVDLELSPGVTVLVGSNGLGKTNLVEAIG
                     YLSSLSSHRVSSDAPLLRFGTEQALIRGRLVRGAQKVTVEVEINGTRANRARINRANP
                     IRSRDVLGLCRSVLFAPEDLALVKGDPASRRRFLDDLLVVLMPRHAATRQEYDKVLKQ
                     RNALLKSARSARFSRGGVTEGHLSTLDVWDAHLA"
BASE COUNT          213 a          393 c          381 g          207 t
ORIGIN      
        1 gaaaaaaaca ttgggttcat ggacgttggc gtgtccggcg gcgtctgggg actgaaaaac
       61 ggttatggcc tcatggccgg cgggtccgcc caacatgtgg agtgggccct gcccatcctg
      121 gatgcactgc gccccgaggg cgaccgcgcg gacagcttcg tccatgtggg cgatgtcggc
      181 gcgggtcact acgcgaaaat ggtgcacaac ggcattgaat acggcctgat gcaggcctac
      241 gccgaaggct atgagctgct gtccaagaag gaaatcatca aggaccttcc cggcacgttc
      301 cgtgcctggc agaagggaac cgtggtccgt tcgtggctgc tggacctgat ggtcaaggcg
      361 ctggacgagg atccgggcct ggagaccatc gacgactacg tggaggattc cggcgaggga
      421 cggtggaccg tggaggaggc catcgccaac gcggtgcccg cgccggccat cacggccgcc
      481 ctttttgccc gcttctcctc acgcgaggac aattcccccg ccatgaagat ggtctcggcc
      541 ctccgccacc agtttggcgg ccacgccacc aagccgtcgg gttcctgaag aagccaggct
      601 aagcagtacc cgtgtatctt gagcatcttt ccctgacgga tttccgcagc tacgcacagg
      661 tggatctgga gctttcaccc ggggtgaccg tcctggtggg ttccaacggg ctcggcaaga
      721 ccaacctggt cgaggccatc ggctacctct ccagcctgtc ctcacaccgg gtcagctccg
      781 acgccccgct cctgcgcttc ggcaccgagc aggcgctgat caggggacgg ctggtgaggg
      841 gggcccaaaa ggtcaccgtc gaagtggaaa tcaatggcac ccgggcaaac cgcgcacgca
      901 tcaaccgggc caatcccatc cgttcccggg acgtcctggg cctgtgccgc agcgtactct
      961 tcgccccgga agacctggcc ctggtcaagg gggacccggc cagccggcgt cgtttcctgg
     1021 acgacctgct ggtggttttg atgcccaggc acgccgccac ccgccaggag tatgacaagg
     1081 tcctcaaaca gcgcaacgcc ctcttgaagt ccgcacgctc ggcccgtttc tcccgcggcg
     1141 gcgtcaccga gggccacctg tccaccctgg atgtgtggga tgcccacctg gctg
//
LOCUS       DASTJJ010000168         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1371906, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000168 DASTJJ010000000
VERSION     DASTJJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371906"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..828)
                     /locus_tag="VFR02_02075"
     CDS             complement(289..828)
                     /locus_tag="VFR02_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9869274.1"
                     /translation="MSETNASPLPVFVYDVFLKDAVKHTQIAPFVRTWARAEVPGKLY
                     QLPTGLPILVEEEPAAAPAAAAPAPAGAPAAPAAPAPAAAPPAKVFGELMGFNQSDRV
                     LALIDAQEGFRPDAPESSRLLRVEREVTVAATGEKVKAWVYVFPKEKFNAKELYAVHA
                     HGGDWRKFVMMPRFDGYEH"
     gene            complement(956..1339)
                     /locus_tag="VFR02_02080"
     CDS             complement(956..1339)
                     /locus_tag="VFR02_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9869275.1"
                     /translation="MFKIEAVVRPDKLDSVRQKLEGIGYPGVMVTEMSGHGRQKGLHS
                     GWPDSLRVSFLPKVKLEIVVDKKHLSAVVDAIVKGAHTGEMGDGKIFISEIIDAVRIR
                     TGERGVKAISMVEDARRPRRIAGLR"
     gene            complement(1432..1773)
                     /locus_tag="VFR02_02085"
     CDS             complement(1432..1773)
                     /locus_tag="VFR02_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9869276.1"
                     /translation="MKKIEAIIRPEKFEEVKAALEEAGYGGITVTEVEGHGKQKGITQ
                     QWRGRKYKVELLPKLKLEIVASSKDAVRLIKAILKSGSTGQVGDGKIFVYGIEEAYKI
                     SSGEEGEKVVS"
     gene            complement(2387..3565)
                     /locus_tag="VFR02_02090"
     CDS             complement(2387..3565)
                     /locus_tag="VFR02_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9869277.1"
                     /translation="MNKTIKALSLVASLALMGAAPVLADTAAPTVKVDGLVDAYYTYN
                     FTNSANKVQGAGNSSAYWYNTTDNSYSLGLAEAKITATQGAATGVVELAYQDVAGLTP
                     LVDSTGTGIAGVGVLQAYASYNTGAWTITGGRFVTWIGNEVVEASSNWNYSHSLLFWY
                     TIPVWNQGISVGLAVDPTLSITGYATNGWNAATNTASFTAQTFGAQLAWTPSSMWKVV
                     LNGIDGPGNYNGFDRWVGEAIIDYNPTSDWSFALDATYGGQDLGSSIATNLNGSSTNL
                     TSDDIWGVDLYGKYAISSDWTAALRLEEVKDDANAYGLYQSATNFGAATAKTGEGREA
                     TLTLTHAFTPAWSCSLEGRYDYVVVNGKVPAGTAGDPGPFAANSADQLTATLSTAFVF
                     "
     gene            complement(3894..>4265)
                     /locus_tag="VFR02_02095"
     CDS             complement(3894..>4265)
                     /locus_tag="VFR02_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869278.1"
                     /translation="PVKKGLDERLPNGVPRWRYRVANLTLWKYESRDKGILEAAAPQD
                     AGDFRVLTPEGVSFGSDSGEVRDACENAYFKYDGVGENGKYRILKYTHGEGLAFADKL
                     EFWMDRDGKMQKIRFGTLDEH"
BASE COUNT          855 a         1310 c         1270 g          830 t
ORIGIN      
        1 aacaccccca ggaaggcgaa gatcccgctg accgccgcct gtcgggaggg tttttcccac
       61 caatgggcgg tccggggcag gaaaggcagc aacaccagca gggtcaggag gagggccagg
      121 ggggccccgt ccaggaccac ccgcaggggg ctgaggacgg ggacggtcgc gctttccaag
      181 ggtcagcctt tccccggacg ggatccggtg tggtttctcc gggggaacgg gtccccggga
      241 acggcaaggg gcctcgggag ccccttcagg cggggctcag gggacggctt agtgttcgta
      301 gccgtcgaag cggggcatca tgacgaactt gcgccaatcg ccgccgtggg cgtgcacggc
      361 gtaaagttcc ttggcgttga acttttcctt ggggaacaca tagacccagg ccttcacctt
      421 ttcgccggtg gcggccaccg tcacttcccg ctccacccgc aagaggcggc tggattcggg
      481 ggcgtcggga cggaagcctt cctgggcgtc gatcagggcc aagacccggt cggattggtt
      541 gaaacccatc agttccccga aaaccttcgc cggcggggcg gctgccgggg ccggggcggc
      601 aggcgccgcg ggcgccccag cgggcgcggg agcggcggcc gcaggggcgg cggcaggctc
      661 ttcctccacc aaaatgggca ggccggtggg caattggtac agtttgcccg gcacctcggc
      721 ccgggcccag gtgcgcacga agggggcgat ctgggtgtgt ttgacggcgt ccttcaagaa
      781 aacgtcatag acaaaaacgg gaagggggga tgcattggtc tcgctcacgc ggtcctccaa
      841 gggctggcac tggaggccta tggtactaag ggcggggacg aaaatccacc cgctttagtc
      901 ggggtgaggg tggatggaac tgagacactg cctaggcggg atcccaggca atgggtcagc
      961 gcagaccggc gatgcggcgg gggcggcggg cgtcttccac catggaaatg gccttgacgc
     1021 cccgttcccc cgtgcggatg cgcaccgcgt cgatgatctc cgaaatgaag atcttgccgt
     1081 cccccatctc gcccgtatgg gcgcccttga cgatggcatc caccacggcg gacaggtgct
     1141 tcttatcgac cacgatctcc agcttgacct tgggcaggaa actcacccgc agcgagtcgg
     1201 gccaacccga atggaggccc ttttggcggc catggcccga catttcggtg accatgacgc
     1261 ccgggtagcc gatgccttcc aatttttggc ggaccgaatc cagcttgtcc ggcctcacca
     1321 ccgcctcgat cttgaacatg accgcccgcc tttgaggagc ctgataaata tttggggccg
     1381 accttatcca ggccgacccc aagccctgcg ggtggagagg agagagaagg gttacgaaac
     1441 gactttttcg ccctcttcac cgctgctgat cttgtaggcc tcttcgatac cgtacacgaa
     1501 tatcttgccg tcccccacct gtccggtgga cccgctcttc aggatggcct tgatgagccg
     1561 gacggcgtcc ttgctgctgg ccacgatctc caacttcagt ttcggcaaca actccacctt
     1621 gtacttgcgc ccccgccact gctgggtgat ccccttctgc ttgccgtggc cctccacctc
     1681 ggtcacggtg atgccgccgt atccggcttc ctcgagggcg gctttgacct cctcgaactt
     1741 ctccggacgg atgatggctt caattttttt catgtggcct ttgaccccac ctcgttagtt
     1801 cgaacttcgg ccctactata ccccatcccg tcccggaggg gcccctaccg gggccgccgc
     1861 cgaaaaaccc gggccggaat gggccgccaa aggggcatgc cggccccggg gatcccccag
     1921 atcaacgccc tgatgaacct gaaccattcc atggccgccc cctttttcag caaggataaa
     1981 agcaagactt gtgccaggga cgccggaaaa tttcgggctg gaatttgttc aagaaaaggc
     2041 ttcgaggcaa aataaaccaa gagcattttg gcggggacct acattttcgt ccgacaaaag
     2101 gaagcaatga ttacaatccg gcatcggctg tttggcggcg ggggaaaaga tccaggggct
     2161 tcttccgcgg ccggttatcg acggccgtcc ccgcccctca aagatcgggt gaacgataaa
     2221 gcagaaccat gcttttggcg aggcatttaa agcccccccc agtccccccg ctgggggagg
     2281 tgggacaccg gtgaaaatgg tttgctttgc cgggagcatg ctcccgaaat aaaaaaagcc
     2341 ccgcccccca aagggggacg gggctttgaa ctgcaatgag acggtattag aacacgaacg
     2401 cagtcgacag cgtggcggtc aactgatccg cgctgttggc cgcaaagggc cccggatcgc
     2461 cggcggttcc agcaggaacc ttcccgttga ccacgacgta gtcataacgg ccttccaacg
     2521 agcaagacca agccggggtg aaggcgtggg tcagcgtcag agtggcctcg cggccttctc
     2581 ccgtcttagc cgtcgccgcg ccgaagttgg tggccgactg gtaaagacca taggcattgg
     2641 catcatcctt cacttcttcc aagcgaagag ccgccgtcca atcgctggaa atggcatact
     2701 taccgtacaa atcgacaccc cagatatcat ccgaggtcag attcgtagag ctgccattca
     2761 aattggtggc gatcgagctt cccaggtcct ggccgccgta agtggcgtcc aaggcgaaag
     2821 accaatcgga agtcgggtta taatcgatga tggcttcacc gacccaccga tcaaagccat
     2881 tgtaatttcc gggaccatca atgccgttca aaaccacttt ccacatgctg gacggggtcc
     2941 aagccaactg ggccccaaag gtctgggcgg tgaaggaagc cgtgttggtg gcggcgttcc
     3001 aaccgttggt ggcgtaaccg gtgatcgaca aggtcggatc cacggccaag ccaacgctaa
     3061 tgccttggtt ccaaaccggg atggtgtacc agaacaggag cgagtggctg tagttccagt
     3121 tgctggaagc ttccaccact tcgttgccga tccaggtcac gaagcggccg cccgtgatcg
     3181 tccaagctcc cgtgttgtag gaggcgtagg cttgaagcac cccaacaccg gcgatacccg
     3241 tgcccgtcga gtcaacaagc ggggtcaaac cggcaacatc ttggtaagcc aactccacca
     3301 caccggtggc ggcaccttgg gtcgcggtga ttttcgcttc cgccaaaccc agagagtagc
     3361 tgttatcggt cgtgttgtac caataagcgc tagagtttcc cgcaccttgc accttgttcg
     3421 ccgagttggt gaagttgtac gtatagtacg catccaccaa gccgtcgacc ttcacggtcg
     3481 gagcagccgt atcggccaga acgggcgcgg cgcccatcag ggccaacgag gccactaaag
     3541 acaaggcttt aatagtcttg ttcatctctt cctcctgaaa gtttaagctg acctgctctc
     3601 tctcaccttt agccctcctg aacttcttca gggacagaag ggccctctac atgaggatgg
     3661 gttatagcag agcaaagcct gtgccaaaag gtgggagacc ggggaatgcc gcaaatggaa
     3721 gccatttggg cttttggggc ccttggaaag tcctgtgttt gcaaggcttt ccaagtgcaa
     3781 ttcaacgatt atggcgcaaa cctacaaatt tgtttttcgg ccaattttta cgggttctta
     3841 acgccgaacg aacacatccg acaccccgtg acggaatgga ttttcgatcc ggctcaatgt
     3901 tcatccaagg tcccgaagcg gatcttttgc atcttgccgt cgcggtccat ccaaaactcc
     3961 agcttgtccg cgaaagccag cccctcgccg tgcgtgtatt tcaggatccg gtatttcccg
     4021 ttttccccca cgccgtcata tttgaaatag gcgttctcgc aggcatcgcg cacctccccg
     4081 ctgtccgacc cgaaagagac cccttcgggc gtgaggacgc ggaaatcccc cgcatcctgg
     4141 ggggcggcgg cctccaagat ccccttgtcc cgcgactcat acttccagag ggtcaagttg
     4201 gcgacgcggt accgccagcg cgggaccccg ttgggcaggc gctcgtccaa ccccttcttg
     4261 accgg
//
LOCUS       DASTJJ010000169         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1738821, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000169 DASTJJ010000000
VERSION     DASTJJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738821"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFR02_02100"
     CDS             complement(<1..361)
                     /locus_tag="VFR02_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9869279.1"
                     /translation="MDIAARMKQIAPSMTLAIDAKAKKLKEEGQDILNFGAGEPDFNT
                     PQVACDAAKKAIDEGHHKYTPAAGTLELRKAVGAYLDREYKVQYSPNDIVISNGAKHS
                     LYNAILALVDPGDEVLIP"
     gene            complement(536..>1659)
                     /locus_tag="VFR02_02105"
     CDS             complement(536..>1659)
                     /locus_tag="VFR02_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9869280.1"
                     /translation="LNAEKLAARHKGVDVIDFGMGNPDNPTPQPIVDKLIEVVRDRKS
                     HRYSTSKGIPAFLKAVSRWYERKYNVPIDPKTEAVSVIGSKEGLAHLGLALFNRGDKI
                     LVPNPTYPIHHYSIIIAGGKIVSVPIIEGPDVFFKNLEKAYKKSTPKPKVLIINFPHN
                     PTTVSVDQEYFKDIVKWAKKHQVTVVHDLAYADIVFDGYKSPSFLSVKGAKDVGVEVF
                     TMSKSYNMAGWRIGFAAGNPKVIGALAKIKSYMDYGIFTPIQVAAITALDSPEENLRK
                     LAAVYQERRNLMVEGMNQMGWPVEKPRAAMYLWAKIPPKFAKMGALDFSMMLLKEAGV
                     AVAPGTGFGDLGEGYVRLALVENKDRSRQALRNMKKLFS"
BASE COUNT          279 a          492 c          519 g          369 t
ORIGIN      
        1 aggggatgag cacctcgtcg ccggggtcca ccagggccag gatggcgttg tagagcgagt
       61 gcttggcgcc gttggagatg acgatgtcgt tgggcgaata ttggaccttg tactcgcggt
      121 cgaggtaggc ccccaccgcc ttgcgcaatt ccagggtccc ggcggcgggc gtgtatttat
      181 ggtgcccctc gtcgatggcc ttcttggccg cgtcgcaggc gacctggggc gtgttgaagt
      241 cgggctcccc ggccccgaaa ttgaggatgt cctgcccctc ttccttcagc ttcttggcct
      301 tcgcgtcgat ggccaaggtc atcgaggggg cgatctgctt catgcgagcg gcaatgtcca
      361 ttttctatat ctcctgaaat tcgaagctcc aaagaaaccc attccaccac aagctccctt
      421 acgggagcca ctaagttcac gaaatcaaac gaacttgatt ttatttctat ccaaatccaa
      481 ttttcaactt cgtgcacttc gtggccgccg aaggcggctc gtggtgaaaa tggttttacg
      541 agaacaactt cttcatgttc cgcagggcct ggcggctacg gtccttgttc tccaccaggg
      601 ccaggcgcac atagccctcg cccaggtcgc cgaaacccgt gccgggggcc acggccacgc
      661 cggcttcctt cagcagcatc atggaaaagt ccaacgcgcc catcttggcg aacttgggcg
      721 ggatcttggc ccagaggtac atggcggccc ggggtttttc caccggccag cccatctggt
      781 tcatgccctc caccatcagg ttgcggcgtt cctggtagac ggcggccagc ttccggaggt
      841 tctcctccgg gctgtcgagc gccgtgatgg cggccacctg gatgggggtg aagatgccgt
      901 agtccatgta gcttttgatc ttggccaggg ccccgatgac cttggggttg ccggcggcga
      961 acccgatgcg ccagcccgcc atgttgtagg acttggacat ggtgaagacc tccaccccca
     1021 cgtccttggc gcccttcacc gacaggaagc taggcgactt gtagccgtcg aagacgatgt
     1081 ccgcgtaggc caggtcgtgc accacggtca cctggtgttt cttggcccac ttcacgatgt
     1141 ccttgaagta ttcctggtcc acgctcaccg tggtggggtt gtgcgggaaa ttgatgatga
     1201 gcaccttggg cttgggcgtg gacttcttgt aggccttttc caggttcttg aagaaaacgt
     1261 ccggcccctc gatgatcggc acggagacga tcttcccgcc cgcgatgatg atgctgtagt
     1321 ggtggatggg ataggtgggg ttgggcacca ggatcttgtc gccccggttg aagagcgcca
     1381 agcccaggtg ggccaggcct tccttggaac cgatgaccga gaccgcctcg gtcttggggt
     1441 cgatggggac gttgtacttg cgctcgtacc agcgcgacac ggccttcaag aaggccggga
     1501 tgcccttgga ggtcgaatag cggtgggact tccggtcccg caccacctcg atgagcttgt
     1561 ccacgatggg ctggggcgtg ggattgtccg ggttgcccat gccgaagtcg atgacgtcca
     1621 cgcccttgtg ccgggccgcc agtttttcgg cgttcagcc
//
LOCUS       DASTJJ010000170         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_883271, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000170 DASTJJ010000000
VERSION     DASTJJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883271"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFR02_02110"
     CDS             complement(<1..827)
                     /locus_tag="VFR02_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9869281.1"
                     /translation="MKRTPPPWTGEKLYFKGDDYFRDLLAAIRGARQNLDFETYLFEP
                     GRLGDRLVAALAGAARRGVRVRLLVDGVGSPEFAGHYGPGLARAGAAFRVYRSWPVIF
                     STARRLLGWGRLGRALRQAHGLLSSGNRRDHRKLCLVDDRQVWMGSFNVSDWNLAELK
                     GSAAWRDTGLRLSGVAGTVFRQAFRLAWEDRWPRPWGRRRVRRIKRWIVREVVGGPIR
                     LTLTRRLRAAYRRELTERFQLARRRIWILTPYFTPTRSLLRALARAARRGCDVRLVLP
                     "
     gene            complement(861..>1126)
                     /locus_tag="VFR02_02115"
     CDS             complement(861..>1126)
                     /locus_tag="VFR02_02115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9869282.1"
                     /translation="ETDILGRLGGDEFVGMLNLAGEVNETTLRQRFEGALEEHNGFHG
                     RSFKLSVSLGFSVYDPREPGSAEDLLARADALMYERKKAKPGG"
BASE COUNT          215 a          423 c          326 g          162 t
ORIGIN      
        1 ggcaggacca gtcgtacgtc acagccacgc cgggcggccc gggccagggc ccgcagcagc
       61 gatcgggtcg gggtgaaata aggcgtcaag atccagatcc gtcggcgggc cagttggaaa
      121 cgttccgtca attcccgtcg ataagcggcc ctcaggcgcc gggtcagggt gagccggatc
      181 ggccccccca ccacctcccg gacgatccaa cgcttgatcc gccgcacccg ccgccggccc
      241 caaggccggg gccaacggtc ctcccaagcc aggcggaaag cctgccggaa aaccgtgccg
      301 gccacccccg acagcctcaa gcccgtatcc cgccaagccg cgcttccctt cagttccgcc
      361 aaattccaat ccgagacgtt gaaactgccc atccaaacct gccggtcgtc caccaggcaa
      421 agcttccggt ggtcccgccg gttgccgctg cttaaaagcc catgggcttg acggagggcc
      481 cggcccaacc ggccccaacc caaaagacgc cgggcggtcg aaaagatgac gggccacgag
      541 cgatagaccc ggaaagccgc gccggcgcgc gccaatccgg gcccataatg gccggcgaat
      601 tccggggaac ccaccccatc caccaacaat cggacccgga ccccccggcg ggcggccccg
      661 gccagggccg ccacaagccg gtcccccagg cggccgggct cgaaaagata ggtctcaaaa
      721 tccaggtttt gacgggctcc gcggatggcc gccaagaggt cccggaaata atcgtcacct
      781 ttaaagtaaa gtttttcccc tgtccaaggg ggcggcgtcc tcttcatcgt ttggatcctc
      841 cccatggtcc cggtccgcgt tcatcccccc ggcttggcct tcttgcgttc atacatgagg
      901 gcatccgccc gggcaagcag gtcctccgcg gaacccggct cccgggggtc atagaccgaa
      961 aagcccaggc tgaccgagag cttgaagctg cgcccatgga acccgttgtg ctcctccaag
     1021 gccccctcaa accgctgccg gagggtcgtt tcattcactt ccccggccaa gttgagcatc
     1081 cccacgaact cgtccccgcc caggcggccc aggatgtcgg tctccc
//
LOCUS       DASTJJ010000171         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_27084, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000171 DASTJJ010000000
VERSION     DASTJJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27084"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /gene="panD"
                     /locus_tag="VFR02_02120"
     CDS             complement(<1..348)
                     /gene="panD"
                     /locus_tag="VFR02_02120"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET9869283.1"
                     /translation="MSRTFVRSKIHQATVTQTDLRYEGSITLDPKLMAAADIAPYEQV
                     HVLNLNTGSRLETYAIQGKPGSGVVCLNGAAARHAEVGDQVIVLTYESFAGAPPKGYA
                     PTVVHVTDRNRVKK"
     gene            complement(417..1259)
                     /gene="panC"
                     /locus_tag="VFR02_02125"
     CDS             complement(417..1259)
                     /gene="panC"
                     /locus_tag="VFR02_02125"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9869284.1"
                     /translation="MKVISDPKAMQALSRKWKRAGKRVGFVPTMGALHEGHLALVRAA
                     RKADGLVAASIYVNPLQFGPQEDLKQYPRTLEADLALLKKEKVAAVFTPSDKVMYPEG
                     YAARVQVTGPLVEGLCAPFRPGHFDGVATVVVKLLGCVQPTRLYLGQKDAQQALVLRQ
                     VIADLDLDVETVLCPIVREHDGLAMSSRNVRLTPEGRRAAPVLYRALKVGKGVFELGV
                     TDPGKVMAEMRKVLKEEKRAKLQYLEAVETPSLRPAAQLKPGTLLALAAFIDQVRLID
                     NLIL"
     gene            complement(1376..>1612)
                     /locus_tag="VFR02_02130"
     CDS             complement(1376..>1612)
                     /locus_tag="VFR02_02130"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9869285.1"
                     /translation="LVPAETAQAITRALRIPTIGIGAGPHCGGQVQVLHDLLGLLPDF
                     HPKHAKAYLNLHEAIRDAIRQYADEVRKGQFPKA"
BASE COUNT          261 a          496 c          558 g          297 t
ORIGIN      
        1 ttttttcacc cggttgcggt cggtcacgtg caccacggtg ggcgcgtaac ccttgggcgg
       61 cgcgccggcg aagctctcat aggtcagcac gatgacctgg tcccccacct cggcgtgccg
      121 ggcggcggcg ccgttgaggc agaccacgcc cgagccgggc ttgccctgga tggcgtaggt
      181 ctccaggcgg gacccggtat ttaggttcag cacgtggacc tgctcgtagg gcgcgatgtc
      241 ggcggcggcc atcagtttgg ggtccagcgt gatgctgccc tcgtagcgca ggtcggtctg
      301 ggtgacggtg gcctggtgga tcttggaacg gacgaaggtg cggctcatgg tttcccttat
      361 tttaaagtcc cgtaaaaagg ccgtaaaacc gggataagcg agggattaac ttaagctcac
      421 aggatgagat tgtcaataag ccgcacttgg tcgataaaag cggccaaagc cagcagggtg
      481 ccgggtttca gctgggccgc gggccgcaag gaaggggttt ccacggcctc caggtattgg
      541 agcttggccc gtttctcctc cttcagcacc ttgcgcatct cggccatcac cttccccggg
      601 tcggtcacgc ccagctcaaa gacgcccttc cccaccttca gggcccggta gaggacgggg
      661 gccgcccggc ggccctcggg ggtcaggcgg acgttgcggg agctcatggc caggccgtca
      721 tgctcgcgca cgatgggaca gaggaccgtt tccacgtcca ggtccaggtc ggcgatgacc
      781 tggcgcagca ccagggcctg ctgggcgtcc ttctggccca ggtagagccg ggtgggctgc
      841 acgcaaccca ggagcttcac caccacggtg gccacgccgt cgaaatgccc ggggcggaaa
      901 ggcgcgcaga gcccctccac cagggggccg gtgacctgga cccgggcggc atagccttcc
      961 ggatacatga ccttgtccga gggggtgaag accgccgcca ctttttcctt tttcaacagg
     1021 gccaggtcgg cctcgagggt ccggggatat tgcttgagat cctcctgggg gccgaactgc
     1081 aatgggttca cgtagatgga ggccgccacc aggccgtcgg ccttgcgggc ggcgcgcacc
     1141 agcgccaagt gcccctcgtg cagggcgccc atggtgggga cgaagccgac ccgcttcccc
     1201 gcgcgtttcc acttgcggga cagggcctgc atggccttgg gatcggaaat gactttcatg
     1261 gatacctcgg aaaaacccgt tctcaccgca aagacgcgaa gccgcaaaga gcgtcagatc
     1321 ctattttctt ttcgtcggtc aacttagcgt ctttgcgcct ttgcggtgaa ccgggtcaag
     1381 ctttcgggaa ttggcccttg cggacctcgt cggcgtattg gcggatggcg tcccggatgg
     1441 cctcgtgaag gttcaggtag gccttggcgt gcttggggtg gaagtcgggc agcaggccca
     1501 gcaggtcgtg cagcacctgc acctggccgc cgcagtgggg gccggccccg atgccgatgg
     1561 tggggatgcg cagcgcccgg gtgatggcct gggccgtctc ggccgggacc ag
//
LOCUS       DASTJJ010000172         1343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1249096, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000172 DASTJJ010000000
VERSION     DASTJJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249096"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFR02_02135"
     CDS             complement(<1..328)
                     /locus_tag="VFR02_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9869286.1"
                     /translation="MGSKSSKRQRKNYELVDRTKAYPLPEAVGILKKAAPAKFNETIE
                     LHIRLGVDPKKADQQVRGTVSLPHGTGKTLRVAVLAKGEKIKEAEAAGADFAGDNDFI
                     EKIAAGF"
     gene            complement(421..846)
                     /gene="rplK"
                     /locus_tag="VFR02_02140"
     CDS             complement(421..846)
                     /gene="rplK"
                     /locus_tag="VFR02_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9869287.1"
                     /translation="MAKEINAMVKLQIPAGKANPAPPVGPALGQHGVNIMEFCKQFNA
                     ATEKQAGLIIPVVITIYKDRSFTFITKTPPASVLLIKAAGLEKGSAVPNKTKVGKVTA
                     KQVEEIAKTKMPDLNAQKLDSAVRMVEGTARSMGIEVTK"
     gene            complement(938..>1343)
                     /gene="nusG"
                     /locus_tag="VFR02_02145"
     CDS             complement(938..>1343)
                     /gene="nusG"
                     /locus_tag="VFR02_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9869288.1"
                     /translation="EEVTETRAGKKRVTNKKFFPGYILVEMDMDENSWSVVRNTPKVT
                     SFVGSGNNPIPLPDEEVTAILEHIGKRRERRALKSQFDKGDLVKVMDGPFSNFSGTVE
                     DINPERERVRVMVTIFGRQTPVELEFHQVEKE"
BASE COUNT          202 a          423 c          405 g          313 t
ORIGIN      
        1 ggaacccggc ggcgatcttt tcgatgaagt cgttgtcccc ggcgaaatcg gcgcccgcgg
       61 cctcggcttc cttgatcttc tcgcccttgg ccaacaccgc cacgcgcagg gtcttgcccg
      121 tgccgtgcgg caggctgacg gtgccgcgca cctgctggtc ggccttcttg gggtccacgc
      181 ccaggcggat gtggagctcg atggtttcgt tgaatttggc gggggcggct ttcttcagga
      241 tgccgaccgc ttcaggaagg ggataagcct tggtccggtc caccaactcg taattcttgc
      301 gctgcctttt gctcgacttg ctgcccatga tttcctcccg tggtgcgaac gtccgcctcg
      361 aaggcgcgac tcccacagtt aatgcccccg aggggggaac gtcgtcgact tcgccgtcgg
      421 ctacttggtg acctcgatgc ccatgctgcg ggccgtgccc tccaccatgc ggaccgcgga
      481 gtccagcttc tgggcgttca ggtccggcat cttggtcttg gcgatctcct cgacctgctt
      541 ggccgtgacc ttgcccacct tggtcttgtt ggggacggcc gaaccctttt ccaggcccgc
      601 cgccttgatc aggagcacgg aagccggggg ggtcttggtg atgaaggtga agctgcggtc
      661 tttatagata gtgatgacca ccgggatgat gagcccggcc tgcttttcgg tcgccgcgtt
      721 gaactgcttg cagaattcca tgatgttgac gccgtgctgc cccaaagccg gacccaccgg
      781 cggggccgga ttggccttgc ccgcggggat ctggagcttc accatcgcgt tgatctcttt
      841 tgccattgcc ctcaaccttc ctctaagctc aaaagcttct taaattccgg cgcctggcgg
      901 ccggcctcaa acaagagcgg tgattgtagc cgctttccta ctctttttca acctggtgga
      961 actcaagttc cacgggggtt tggcggccga aaatggtcac catgacccgc acccgctccc
     1021 gctcggggtt gatgtcttcc accgtgcccg agaagttcga gaaggggccg tccatgacct
     1081 tgaccagatc ccctttatcg aactggcttt tcagggcccg gcgctcccgg cgcttgccga
     1141 tatgctccaa aatggccgtc acttcctcgt cgggcagggg gatggggttg ttgccggaac
     1201 ccacgaaact ggtgactttg ggggtgttgc gcaccacgga ccaggaattc tcgtccatgt
     1261 ccatctccac caggatatag ccggggaaga acttcttgtt ggtgacgcgt ttcttgccgg
     1321 cgcgggtctc cgtgacctcc tcc
//
LOCUS       DASTJJ010000173         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1739301, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000173 DASTJJ010000000
VERSION     DASTJJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739301"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFR02_02150"
     CDS             <1..1084
                     /locus_tag="VFR02_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9869289.1"
                     /translation="EVRRAAAIALRETRDPASLPPLFQAAAGDDDPLVRKLALEGMRA
                     TGDRRILPPAQKALGDRHPDVRQAALEILAQFPDPSHRPFLLGALTDPEEDLAERSAR
                     LLGETGDPRSVPELLGLLEDLRPQVRARAASVLGRLGDPSVIPALGKLVLEETGPPAR
                     RALGSLSLLRSHQALPWIVLGLANPDFMVHQESNRLLQEIPYSRIPDLLINSLEDLDP
                     TVRLTAARALGWMKDRGPRPPLTAAGGKTPIRAYVRPLGRRYARSKAARGSRPAWRRP
                     SSWVVCWLGAGFFETPAKDTVLWADRPSSLPPCLPRRLPDPFSAQVRLKRPRLYALDT
                     KLLFLNPSVKKRRAPKPFRSHRPLSQ"
     gene            complement(1467..1895)
                     /locus_tag="VFR02_02155"
     CDS             complement(1467..1895)
                     /locus_tag="VFR02_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9869290.1"
                     /translation="MKRERFLWPLTQSHHGALLLAKRVREQLPGTAGDPTALSRLAAE
                     VRGQFQRELGPHFGQEGEILEVFGRRVGAGDPDILRILDEHRKMEAWAGEGSAEALAA
                     FAETLTRHIRFEEEVLFPRWETLFTEDEKRWVSQRLGGSR"
     gene            complement(1901..>2206)
                     /locus_tag="VFR02_02160"
     CDS             complement(1901..>2206)
                     /locus_tag="VFR02_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9869291.1"
                     /translation="GDEWEGLLAPLDHAPTRACALAERSFLRALQGGCRVPVGALARW
                     EGESLALSGMIADPAQGRTLRGRSTGPAGRPQELGERLAQDLLAQGAGEILRRFGRD"
BASE COUNT          359 a          779 c          693 g          375 t
ORIGIN      
        1 ggaggtccgc cgggccgccg ccatcgccct gcgggaaacc cgggatccgg cctctcttcc
       61 cccgcttttc caggcggccg ccggggacga cgaccccttg gtgcgcaagc tcgccttgga
      121 aggcatgcgg gcgaccggcg accggcggat ccttccgccc gcccaaaagg ccttgggcga
      181 ccgccacccg gatgtgcgcc aagccgccct ggagatcctc gcccaattcc ccgacccctc
      241 ccaccggcct ttcctgctgg gcgcgctcac ggaccccgag gaggacctgg ccgaaagatc
      301 ggcccggctc ttgggggaaa ccggggatcc ccgttccgtc ccggaactgc tggggctctt
      361 ggaggacttg cggccccagg tgcgggctcg ggccgcttcg gtcttgggcc ggctgggcga
      421 cccaagcgtg atcccggccc tgggaaagct ggtgctggaa gagaccggcc cccccgcccg
      481 aagggccctg ggctccctgt ccttgctgcg gagccaccag gccctgcctt ggattgtcct
      541 ggggctggcc aaccccgact tcatggtcca ccaagaatcc aaccgcctgc tgcaggagat
      601 cccctattcc cggatccccg acctgctcat caactcgctg gaagacttgg accccacggt
      661 ccgcttgacg gcggcccggg cgcttggctg gatgaaagac cgcggtccac gaccgccctt
      721 gaccgcagca ggcgggaaga ccccgatccg ggcgtacgtt cggccgcttg ggaggcgtta
      781 cgctcggtcc aaagccgcca gaggttcaag accggcctgg cggcggccgt cctcctgggt
      841 ggtttgctgg cttggggctg gctttttcga aacgccggca aaggataccg tcctttgggc
      901 cgaccggccg tcgtcgcttc ccccctgcct tccacgccgt ctccctgatc cattttcggc
      961 ccaagtccgg ctcaaaaggc cccggcttta cgccctggac actaagttgt tattcctcaa
     1021 tccgtccgtc aaaaaacggc gggccccgaa gcccttccgc tcccacagac ctctctccca
     1081 ataggttccc gaaaaaatcc ggccggatgt gccgactttg catttgaagg gtgttttttt
     1141 gaaagataaa ttggtgggag cgatccttgg acgttcggca aaaacgctca aagccggtcc
     1201 aagcgtgata aaagcgggcc ggtgacagca tggatccaac ggtcaaaaac catatcccca
     1261 ccgctccgga aagagcatcg gccccagctc cggcctgcgg cagggatcac cggcggaccc
     1321 ggaaggtggg gcgaggccgc cggaccggac atccacccct gtccgctttg aaaaccgggg
     1381 cttttattgg atttttgaaa tagttaaagt tggttttcaa gcctttaagc cggttacttt
     1441 cagccttcaa ggaacctgcg gatgggtcag cgggagcccc ccagcctctg ggagacccaa
     1501 cgcttctcgt cctcggtgaa gagggtctcc caccggggga aaaggacctc ttcctcgaag
     1561 cggatatgcc gggtgagggt ctcagcgaag gccgccaggg cctcggcgct tccctccccc
     1621 gcccaggcct ccatcttccg atgttcgtcc aggatccgga ggatgtccgg gtccccggcg
     1681 cccacccggc ggccgaagac ctccaggatc tccccttcct gcccgaaatg ggggcccagt
     1741 tcccgttgga attgtccccg gacctccgcc gccaaccggg aaagggccgt gggatcgccg
     1801 gccgtgccgg gcagctgttc gcgcactctt ttggccagca acagggcgcc atgatgggac
     1861 tgggtgaggg gccagaggaa tctttcccgc ttcacgtggg tcagtcccgg ccgaaacgcc
     1921 ggaggatctc ccccgcgccc tgggccaaga gatcctgcgc caatcgttcc cctaattcct
     1981 gcggacgccc ggcgggtccg gtcgagcggc cccggagggt ccggccttgg gcgggatcgg
     2041 cgatcatgcc cgagagggcc agggactccc cttcccagcg ggccagggcc cccacgggca
     2101 cccggcaccc gccctggagc gcgcgcagga aagaccgttc ggccagggca caggcccggg
     2161 tcggggcgtg gtcgaggggc gccaacaggc cttcccactc gtcccc
//
LOCUS       DASTJJ010000174         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_149280, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000174 DASTJJ010000000
VERSION     DASTJJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149280"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1072)
                     /locus_tag="VFR02_02165"
     CDS             complement(29..1072)
                     /locus_tag="VFR02_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9869292.1"
                     /translation="MESSTWSRWSYRLLLFFAFATTASIAAQNAIWVAVLLWFFGRVR
                     AGRKIDWPRGLFPIGAMELTLAELRKLLAVFVGGTFVCAVYGVWKHMAGIEVRISSFS
                     GYYLIFGGLMMCALLIIAHFLIQNPRQVRYWAALVAIIPAFLLNETRGAWLAFAAGFA
                     VLVWPKHRRLLAAGAVLALAGFFVLPGQLQQRVQSIADWKDQPRLFIWDAGVRIIKDY
                     PVFGIGQGNLGDIYPKYVSPEAREREHGHLHDNYLQIQVQNGLFGSAAYLFWILAYFY
                     AARRNAPAGGEMGSLNLLFVSLFAASLVWGLTQYTFAHQFMYFQAFLLGLQYRLWTLG
                     KPSGSDPVQTAKA"
     gene            1255..>2055
                     /gene="ugpC"
                     /locus_tag="VFR02_02170"
     CDS             1255..>2055
                     /gene="ugpC"
                     /locus_tag="VFR02_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET9869293.1"
                     /translation="MAKVILKDVNKMYGNNVVAVNNVNIEIADKEFLILVGPSGCGKS
                     TTLRMVAGLEELSGGEIRIDDVLVNDVPPKDRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKYPKEEIKQRVHEAANILGIQELLNRRPKELSGGQRQRVALGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAEISKLHTRLQATMIYVTHDQLEAMTMGDRIAVMKDGFVQQIAE
                     PITLYEKPVNKFVAGFIGTPSMNFMEGTLNKKRTGVYFNEENFEIKLPD"
BASE COUNT          448 a          634 c          657 g          316 t
ORIGIN      
        1 cctattgggg gactgccgac gggcgatctc aggccttcgc ggtctggacc ggatctgatc
       61 ccgagggttt gcccagggtc cagagccggt attgcaggcc cagcaggaaa gcctggaagt
      121 acatgaattg gtgggcgaag gtgtactggg tcaggcccca gaccaaggaa gccgcgaaca
      181 gggagacgaa aagcaggttg aggcttccca tttccccccc ggccggggcg ttgcggcggg
      241 cggcgtagaa ataggcgagg atccagaaga ggtaggccgc gctcccgaac aggccgttct
      301 ggacctggat ctgcaggtaa ttgtcgtgca ggtggccgtg ttccctttcc cgggcctcgg
      361 gggagacgta cttggggtag atgtcgccca ggttgccctg gccgatgccg aagaccggat
      421 agtccttgat gatcctcacc cccgcgtccc aaatgaagag cctgggctgg tccttccaat
      481 cggcgatgct ctggacccgc tgctgcaatt gccccggcag gacgaagaaa cccgccaagg
      541 ccaggaccgc ccccgccgcc aggaggcggc ggtgcttcgg ccagaccagg accgcgaatc
      601 ccgccgcgaa ggccagccag gccccccggg tctcattgag caaaaaggcc gggatgatcg
      661 ccaccaaggc cgcccagtaa cggacctggc gcgggttttg gatgaggaaa tgggcgatga
      721 tcaagagggc gcacatcatc aacccgccga agatcaggta gtaccccgaa aaggacgaga
      781 tgcgcacctc gatccccgcc atgtgcttcc agaccccgta gacggcgcaa acgaaggtcc
      841 cgcccacgaa gacggccagc agcttccgga gctcggccaa agtcagttcc atggccccga
      901 tggggaagag gccccggggc cagtcgatct tccgcccggc ccggacccgg ccgaagaacc
      961 acagcaggac cgccacccaa atggcgttct gggcggcgat ggaggcggtg gtggcgaagg
     1021 cgaaaaagag gagcagccga taggaccagc gggaccaggt ggaggattcc atggggggcg
     1081 attataaggc ccaccttccc gggctccaac cccaatcgtt gacagtgccc gggttcgatg
     1141 tttaaataac aaggcttttt cgagcccgcc ggaggaagca ggcgccgggg gtgaaccagg
     1201 gactgggccg cgcctcacga ggacggaccg gttccacgca cggaggcagg caaaatggcg
     1261 aaggtcattt tgaaggacgt caacaagatg tacggcaaca acgtggtggc ggtcaacaac
     1321 gtcaacattg agatcgcgga caaggaattc ttgatcctgg tgggcccctc gggctgcggc
     1381 aaatccacca ccctgcgcat ggtggccggg ctggaggaac tttcgggcgg cgagatccgc
     1441 atcgacgacg tcctggtcaa cgacgtgccc cccaaggacc gggacatcgc catggtgttc
     1501 cagaactacg ccctctaccc ccacatgacg gtcttcgaga acatggcctt cggcttgaag
     1561 ctgcgcaagt accccaagga agagatcaag cagcgcgtgc atgaggccgc caacatcctg
     1621 ggcatccagg aattgttgaa ccgccgcccc aaggaactgt ccggcgggca gcgccagcgc
     1681 gtggccttag ggcgcgccat cgtgcgcaaa cccaaggtgt tcctcttcga tgagcccctg
     1741 tccaacctgg acgccaagct gcgcgtgcag atgcgggccg agatctccaa gctgcatacc
     1801 cggctccagg ccaccatgat ctacgtgacc cacgaccagt tggaggccat gaccatgggc
     1861 gaccgcatcg cggtcatgaa ggacggcttc gtgcagcaga tcgccgaacc catcaccctt
     1921 tacgagaaac cggtcaacaa gttcgtggcc ggtttcatcg gcacgccctc catgaacttc
     1981 atggaaggca cgctcaacaa gaagcgcacg ggcgtttatt tcaacgagga gaacttcgag
     2041 atcaagctgc cggac
//
LOCUS       DASTJJ010000175         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_394422, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000175 DASTJJ010000000
VERSION     DASTJJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394422"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFR02_02175"
     CDS             <1..204
                     /locus_tag="VFR02_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HET9869294.1"
                     /translation="YEFALEIQRQSGLQNCRVVPTLSENILRPAIRPANSAFKLDKAE
                     AAVGHPFRAWPLALAEYLAEVAP"
     gene            201..953
                     /locus_tag="VFR02_02180"
     CDS             201..953
                     /locus_tag="VFR02_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET9869295.1"
                     /translation="MTVPDLSVVIPVYNEQDRIGRTLEESLAFLRSSRTKAEVILVDD
                     GSTDGTVGVVEAFLAKKKAQRVMRILRHPVNRGKGAAVRTGALEARGRVVLYMDADNA
                     TPLSEIVKLLPGLKRGFEVVIGSRATDRSQVKVRQPLYRQAIGRVGNLLVQLLATPGL
                     WDTQCGFKAFSQRAAKVIFPLQTIERFGFDVELLFIARKKGFQVLETSVQWFDAPGSK
                     VHALRDSIRTLGELLLIRWNSWKGAYGAGKRG"
     gene            931..1872
                     /locus_tag="VFR02_02185"
                     /pseudo
     CDS             931..1872
                     /locus_tag="VFR02_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
     gene            1900..>2261
                     /locus_tag="VFR02_02190"
     CDS             1900..>2261
                     /locus_tag="VFR02_02190"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET9869296.1"
                     /translation="MTLLFGLKYAAAGPLFAVLALTLPFHFATKPSVNLLAVRSPRIL
                     TWIYLGMLLANLLANFIVIPRAGASGAAWVWVAGEAGLFLACAWPTWDSLDRKASFRW
                     VPAGLAACAVVGFCISRDP"
BASE COUNT          301 a          758 c          749 g          453 t
ORIGIN      
        1 tacgaattcg ccctggagat ccaacggcaa tcgggcctgc agaactgccg ggtcgtgccc
       61 accctttcgg agaacatcct gcggccggcc attcgccccg ccaattcggc cttcaaactc
      121 gacaaggcgg aggcggccgt cgggcatcct ttccgggctt ggcccctcgc cctggcggag
      181 tacttggcgg aggtggcccc gtgaccgtcc ccgatctttc ggtggtcatc cccgtttata
      241 acgaacagga ccgcattgga cgcaccctgg aggagtcctt ggccttcctc aggtccagcc
      301 gcaccaaggc ggaggtgatc ctggtggacg acggttccac cgacgggacg gtcggggtcg
      361 tggaggcttt cttggccaaa aagaaggccc aacgcgtgat gcggatcctc cgccatcccg
      421 tgaaccgggg caagggcgcg gcggtccgca cgggggccct ggaagcccgg gggcgggtgg
      481 tgctctacat ggacgcggac aacgcgaccc ccctctcgga gatcgtcaag cttttgccgg
      541 ggctcaagcg cggcttcgag gtcgtcatcg gcagccgcgc gacggaccgc agccaggtca
      601 aggtgcgcca gcccctctac cggcaggcca tcggccgggt gggcaacctg ctggtgcaat
      661 tgttggccac ccccggcttg tgggacacgc agtgcgggtt caaggcgttc agccagcgcg
      721 ccgccaaggt cattttcccc ctgcagacca tcgaacggtt cggcttcgac gtggagctgc
      781 tgttcatcgc ccggaaaaag ggtttccaag tgctggaaac ctccgtgcaa tggttcgacg
      841 cgcccggttc caaggtccac gccctgcggg actcgatcag gaccctgggc gaactcctgc
      901 tcatccggtg gaacagctgg aagggggctt atggcgccgg aaagcggggc tgacccgacg
      961 accggagggg cccggcggat cacccgcaac gccctgtcgc tgtccggggc cgagttggtt
     1021 tcccggttct tcacctggct ggccctgttg ctgctcaccc accattggac cttggaagag
     1081 tacggccgtt acgccacggc catcaactgg gtgaacatcc tcctcaacgt cggggggctg
     1141 ggcctgggca ccttggcgat ccgggaggtg gcgcgccgca aggaactggc cggcgagttc
     1201 ctgcacgcca tttctttcct gcgcttctta tcctcggtct tcttcatggc gctcctcgcg
     1261 gcgctggggt gggtcctgca ttacgagccg gtcctgcgca ccgccatggc catcctgggc
     1321 ctgaggctgc ttttcgagtc ccagtcctcc ccttacatca tcatgctgct ggcccacgag
     1381 cgcatgggcc gctaggggtt ggtgtccgcg ggttcgggcc tggtccgcac cctggggttg
     1441 gtcttggtgg tctgggctgg gggcggggtg tacgccgcct gttgggtctg ggtggccgtc
     1501 ggcctggccg ccttcctggt ctactggcgc ctgggccggt cccatgggtg gaagatggaa
     1561 cgctccgggt tcgacgccgc gggggcctgg aggatcctgc tgcgttcggt acccttcgcg
     1621 gccttcagcg tcttccaagc cctttattac aaggtggacg cggtcctctt gaagagcctg
     1681 tccggcaatc aggcggtggc cctctatgac gtggcggggc gcttcctttt cctggtctac
     1741 atggtgtccg accaattctc ggtggccacc ctgcccaccc tctcggccca gcaggagaac
     1801 cgcgctgaac agtcgcgcct cgccacccgg gcgctcaaat tccttctgtt ggcgggatgg
     1861 cccctggcgc tgggcggatg cctgctggcc ggcccgctca tgacgctgct tttcggcctg
     1921 aaatacgccg ccgcgggccc ccttttcgcc gtcctggcct tgaccctgcc cttccatttc
     1981 gccaccaaac cctcggtcaa cctgttggcg gtccgttcgc cccggatact gacctggatc
     2041 tacctgggga tgttgctggc caacctcctg gccaatttca tcgtcatccc ccgggcgggg
     2101 gcctccgggg ccgcttgggt ctgggtggcc ggcgaggcgg ggcttttcct ggcctgcgct
     2161 tggccgacct gggactccct ggaccggaag gcttccttcc gttgggtccc ggcgggcttg
     2221 gccgcctgcg cggtggtggg cttttgcatc agccgcgatc c
//
LOCUS       DASTJJ010000176         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_883677, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000176 DASTJJ010000000
VERSION     DASTJJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883677"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="gltX"
                     /locus_tag="VFR02_02195"
     CDS             <1..1245
                     /gene="gltX"
                     /locus_tag="VFR02_02195"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9869297.1"
                     /translation="GPFGPYRQTERAPLYREKLKLLLSTGAAYPCFCTHKELEAERQA
                     AQFVNQGYRYSGKCRNLPKEEAERRKRDKQPYAVRLRIPEGHTQFDDLIRGEVTVEHT
                     ELDDFILVRSGGEPTYNFVATVDDALMKISHVIRGEDHISNTPKQILLYQALGLPQPR
                     FAHIPLILGPDRAPLSKRHGASSVGEFKRQGYLPEALMNYLALLGWALDGKTELFTKD
                     ALVKAFTLDKVVKSPAAFDYEKLKWMNGHYIREASDERIYQLCLEYLWEADAINEDFM
                     RQGGDALLRMIQTVKSSLKTISDVGEQLTYFLGEVHTYETEALSKYTVADTVPILEKA
                     VLTLEESLVFNAMALEQRFREVAAQSGRKFGEFVHPMRLAITGRSNSPNLFELIEILG
                     QGRCVVRLLRLIQTLRMAAGTP"
     gene            1313..1873
                     /locus_tag="VFR02_02200"
     CDS             1313..1873
                     /locus_tag="VFR02_02200"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9869298.1"
                     /translation="MAHIIEFVFHRGEQKGRLRSVNRNDWAQIVGWWNDKEVRRLFDV
                     HFVDQTFSDAEKWFAKSGSPEDEKLPRSFVIEVEQGGKWLPVGTAEFYSERPSHRTAE
                     FGFLIGDKNWWGKGIGTETAKRMVKYGLEQLGYKRIGAVSAAYNVRSLNALQKAGFQI
                     EGIRKSYVFRDGEYHDQTVMSVIRPD"
     gene            2051..2124
                     /locus_tag="VFR02_02205"
     tRNA            2051..2124
                     /locus_tag="VFR02_02205"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gln,seq:ctg)
BASE COUNT          421 a          686 c          681 g          368 t
ORIGIN      
        1 gggcccttcg ggccctaccg gcagaccgag cgcgcgcccc tctaccggga gaaactgaag
       61 ctgctgcttt ccaccggcgc ggcctatccc tgcttctgca cccacaagga actggaggcg
      121 gagcgccagg ccgcccaatt cgtgaaccaa ggctaccgct attcgggcaa atgccggaac
      181 cttcccaagg aagaggcgga gcggcgcaag cgggacaagc agccctacgc ggtgcgcctg
      241 cgcatccccg agggccatac ccagttcgat gacctcatcc ggggcgaggt gaccgtcgag
      301 cacaccgagc tggacgattt catcctggtg cggtcgggcg gggaacccac ctacaacttc
      361 gtggccacgg tggacgacgc gctgatgaag atctcccacg tcatccgggg cgaggaccac
      421 atttccaaca cgcccaagca gatattgctc taccaggccc tggggctgcc ccaaccccgg
      481 ttcgcccata tccccctcat cctggggccg gaccgggcgc ccctgtccaa acgccatggg
      541 gccagttcgg tgggcgagtt caagcgccag gggtacctgc ccgaggccct gatgaactac
      601 ctggccctcc tgggctgggc cctggacggc aagaccgagc ttttcaccaa ggacgccctg
      661 gtgaaggcct tcaccttgga caaggtggtg aagagcccgg cggccttcga ctacgagaag
      721 ctcaaatgga tgaacggcca ctacatccgg gaggcctccg acgagcgcat ctaccagctt
      781 tgcctggaat atctctggga ggcggacgcc atcaacgagg acttcatgcg ccaaggcggg
      841 gacgcgctgc tgcgcatgat ccagacggtc aagagcagcc tgaagaccat ctcggacgtg
      901 ggggaacagc tgacctattt cctgggggag gtccacacct acgagaccga ggccctgtcc
      961 aagtacaccg tggccgacac cgtgcccatc ctggaaaagg ccgtgctgac cctggaggag
     1021 agcctggtgt tcaacgccat ggccctggaa caacggttcc gggaagtggc ggcccaaagc
     1081 ggacggaagt tcggcgaatt cgtgcatccc atgcgcctgg ccatcacggg caggtccaac
     1141 agcccgaacc tgttcgagct catcgagata ttgggccagg ggcgctgcgt ggtgcggctt
     1201 ttgcgcctca tccagaccct gcggatggcg gcggggaccc cttgagaagg gcggggtcct
     1261 tcggctaagc tggggaagga ccaagacggg cattccaagc caaggggggc atatggccca
     1321 catcatcgaa ttcgtctttc accgcggcga gcagaagggc cgcttgaggt cggtcaaccg
     1381 caacgactgg gcccagatcg tgggctggtg gaacgacaag gaagtgcgcc gcctgttcga
     1441 cgtgcatttc gtcgaccaga ccttctcgga cgccgagaag tggttcgcca agtccgggtc
     1501 ccccgaggac gagaagctgc cccggtcctt cgtgatcgag gtggaacagg gcggcaagtg
     1561 gctgccggtg ggcacggccg aattctactc ggaacgcccc tcccaccgca cggccgagtt
     1621 cggcttcctc atcggcgaca agaactggtg gggcaagggc atcggcaccg agaccgccaa
     1681 acgcatggtc aaatacggcc tggaacagct gggctacaaa cgcatcgggg cggtgtcggc
     1741 ggcctataac gtgaggtccc tgaacgccct gcagaaggcc ggtttccaga tcgagggcat
     1801 ccgcaaaagc tacgtcttcc gggacggcga ataccacgac cagaccgtca tgtcggtcat
     1861 ccggccggat tgagggacat cccccgcccc gtgcggggaa ggggtcaggg tgaggggtgc
     1921 ctcgattccc aggtctggag gggtttttcc cgccttcttt tccgtttggc gccccgtgaa
     1981 cttatgctaa aattccaccc ctgtccggca accgccttgg gaccgggccg tttttcccgc
     2041 cgttgaatct tgggagatcg tctaatggta ggacagcaga ctctgactct gcttatcttg
     2101 gttcgaatcc aagtctccca gccacccaaa cccagccctt ttcggggctg ggtttt
//
LOCUS       DASTJJ010000177         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_516773, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000177 DASTJJ010000000
VERSION     DASTJJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516773"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFR02_02210"
     CDS             complement(<1..990)
                     /locus_tag="VFR02_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9869299.1"
                     /translation="MAPPGQLARLWPRIRPYRGALSIALVTLLLSGGISLAFPQLAGY
                     LVDAAFVRHSRSYLDQIALGLVGLFTVQAALNYIQTYLLTATGERAVAGLRRELFGKL
                     LEMPPGFFADRRIGELTSRLAVDIGLLQAAMSTQAAEFFRQTMTLVGGILILTLSQPH
                     LMLTALFVAPLVVGSAVFFGRRLRKVSKGLQDKVADANAMAEETLSQIRTVQSFVQEP
                     TERGRYTSKIQSIITTALTRAHVRGIFFGVITLVTFWGVTAVLWQGGREVLAGRLTEG
                     DLIKFVLYTLFIAGAVGGLVSLFGSYNEAVGAATRVFELLEQRVEVADPAGPVA"
     gene            complement(1028..>2308)
                     /locus_tag="VFR02_02215"
     CDS             complement(1028..>2308)
                     /locus_tag="VFR02_02215"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9869300.1"
                     /translation="IHWTHRLLAFTFVGFVLALAWAARGRRGAAGLRYQRMTVALLVV
                     SLAQVAVAAAMVLHLLPPGLRALHLFTGTAVWAMLVVLIFFSRRTPAVEAAVAENAPR
                     AVDGPPSVLRDLVTLTKPRIISLLLVTTIFPMFITGHGLPSVGLVVWVIIAGYLMAGG
                     ANAINMWFDRDIDDRMSRTKLRPVPSGRMPAWGALAFGMALGASSFALFWAYVNPLSA
                     WLALAGLLFYVVVYTIWLKRWTPQNIVIGGAAGAFPPLVGWAAMAGRLDLTAIYLFAI
                     IFYWTPPHFWALALIKQGEYAKAGIPMMPLVQGEHRTKLQMLGYTVMLLPLTLLPWLS
                     GDLGLFYAITAVLLGARLLWYCIRLLRESTVTPTAWKMYKYSLLYLFLLFAAMGVDRA
                     VPFGHANRAPEVLNLPAPPSVVGPAVPMEAPHGR"
BASE COUNT          434 a          769 c          811 g          294 t
ORIGIN      
        1 cgccaccggt cccgccgggt cggccacctc cacccgctgc tccaggagct cgaatacccg
       61 ggtggcggcc cccaccgctt cattatatga accgaagagc gacacgagcc ccccgacggc
      121 cccggcaatg aagagggtat agaggacgaa cttgatgaga tcgccctcgg tgagcctccc
      181 ggcgagcacc tcccgccccc cctgccacag cacggccgtg accccccaga aggtgaccag
      241 ggtgataacg ccgaagaaga tcccccgcac gtgggcccgg gtcagggcgg tggtgatgat
      301 ggactggatc ttggaggtat agcggccccg ctcggtgggc tcctgcacga agctctgcac
      361 ggtgcggatc tggctcagcg tctcctcggc catcgcgttg gcatcggcca ccttgtcctg
      421 cagccccttg gataccttgc ggagccggcg cccgaagaag accgccgagc ccaccaccag
      481 cggcgccacg aagagggccg tgagcatcag gtgcggttgg ctgagcgtca agatgaggat
      541 gccgcccacc agcgtcatcg tctggcggaa gaattccgcc gcctgggtgc tcatcgccgc
      601 ctgcaggagc ccgatgtcca ccgccaggcg gctggtcagc tcgccgatcc ggcggtcggc
      661 aaagaatcca ggcggcatct ccagcagttt gccgaagagc tcccgccgaa gccccgccac
      721 cgcccgctca ccggtggcgg tgaggaggta ggtctggatg tagttgaggg ccgcctggac
      781 ggtaaagagc cccaccagcc ccagcgcgat ctggtcaagg tagctgcggc tgtggcggac
      841 gaaggcggca tccaccaaat agccggcgag ctgcgggaag gcgagggaga tccccccgct
      901 caggagcagg gtgacgaggg cgatcgacag cgcgccgcgg taggggcgga tcctgggcca
      961 gaggcgcgcc agttgccccg gcggcgcaat gaatggcttg cgtggctcgg tggctggagc
     1021 ggcggcgtca gcggccgtgg ggggcctcca tcggaacggc cggacccact actgaagggg
     1081 gcgcggggag gttcagcacc tccggggccc ggttggcatg cccgaacggc accgcccgat
     1141 cgacccccat cgcggcaaag agcaggaaga gatagagcag ggagtacttg tacatcttcc
     1201 acgccgtggg ggtcaccgtg gactcccgca gcaaccggat gcagtaccag aggagccggg
     1261 caccgaggag gaccgcggta atggcgtaga agaggcccag gtctccggag agccacggca
     1321 ggagcgtcag ggggaggagc atcacggtgt agcccagcat ctggagcttg gtgcggtgct
     1381 ccccctggac cagcggcatc atcgggatcc cggccttggc gtactccccc tgcttgatga
     1441 gcgccaaggc ccagaagtgg ggcggggtcc agtagaagat gatggcaaag aggtatatgg
     1501 ccgtcaggtc gagccgtccc gccatcgcgg cccacccgac cagcgggggg aatgcccccg
     1561 ccgccccgcc gatcacgatg ttctgcgggg tccagcgctt gagccagatg gtgtacacca
     1621 ccacatagaa caggagcccc gccagggcca gccacgccga cagcgggttc acataggccc
     1681 agaagagggc aaaggaggag gcgccgagcg ccattccaaa ggcgagggcg ccccaggccg
     1741 gcatccgacc cgagggcacc ggccgcagct tggtgcggct catccggtcg tcgatgtccc
     1801 ggtcgaacca catgttgatg gcgttggccc cgcccgccat caggtatccg gcaatgatga
     1861 cccagaccac gagcccgacc gaggggagcc cgtgcccggt gatgaacatc gggaagatcg
     1921 tggtcaccag caggagcgag atgatcctcg gcttggtgag ggtcaccagg tcgcgcagga
     1981 ctgagggcgg gccgtcgacg gcccgcggtg cgttctcggc gacggcagct tccaccgccg
     2041 gggtccgacg cgaaaagaag atcagcacca ccagcatcgc ccagacagcg gtaccggtaa
     2101 agaggtgcag cgctcggagg cccgggggca gaagatgcag gaccatcgcc gccgccaccg
     2161 ccacctgcgc cagcgacacc accaggagcg ccacggtcat ccgttggtag cggagccccg
     2221 cggctccccg gcgcccccgg gcggcccagg ccagcgccag cacgaagccg acgaaggtga
     2281 aggccaggag ccggtgggtc cagtggat
//
LOCUS       DASTJJ010000178         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_271992, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000178 DASTJJ010000000
VERSION     DASTJJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271992"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFR02_02220"
     CDS             complement(<1..282)
                     /locus_tag="VFR02_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869301.1"
                     /translation="MRTLSLVVLLWAGLAPTLCAQSFIPTPPPAKDSSKLGFEKPYEY
                     ERQAYGYDPDGVQYKENQVEDFQVIFITSAPFAALASLGLTGLASTLIEG"
     gene            complement(456..>1120)
                     /locus_tag="VFR02_02225"
     CDS             complement(456..>1120)
                     /locus_tag="VFR02_02225"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET9869302.1"
                     /translation="KTEEPYEQLIVDVPNNCVGVTMEKLGSRKGEMKNMTPLSEVTTR
                     IEFMIPSRCLLGFRTEFLTDTKGEGILHHVFAGYGEFKGEIAGRNRGVLVAFEAGVTA
                     AYGMFQLSDRGKFFIHPRVEVYEGMIVGEHTRENDIAVNPCKTKHLSNMRSKSSDEAL
                     TLEPPTIFSLEQCLEYIQDDELLEVTPQNLRMRKKILNNNERMKVEKMGSKAAQKAMN
                     NA"
BASE COUNT          197 a          333 c          357 g          233 t
ORIGIN      
        1 gccctcgatc agggtggagg ccaggcccgt cagtcccagg ctggccaagg cggcgaaggg
       61 ggcggaggtg atgaagatca cctggaaatc ctccacctgg ttctccttgt actggacgcc
      121 gtccggatcg tagccgtagg cctggcgctc gtactcgtag ggcttctcaa accccaactt
      181 ggagctgtcc ttggccgggg gcggcgtggg gatgaaggac tgggcgcaga gcgtgggggc
      241 cagtccggcc caaagcagca cgacgaggga aagggttctc atacccgcaa tcctatcacg
      301 cccggtcagg agttaggagt gcggagttag gagtgaaaac cgtaaaaacc aggccgtcgt
      361 cgcccaggcc tccgggaaag aatgagctag gatgagcgga aatcagggat caaactcctg
      421 acttccaact cctcactcct aactttttat ctgggtcagg cgttgttcat ggccttctgg
      481 gcggccttgc tccccatctt ttccaccttc atgcgctcgt tgttgttgag gatcttcttg
      541 cgcatgcgca ggttctgggg ggtgacctcc agcagctcat cgtcctggat gtattccagg
      601 cactgttcca gcgaaaagat ggtcggcggc tccagggtca aggcctcgtc ggaggacttg
      661 ctgcgcatgt tggacaggtg cttggtcttg caggggttca ccgcgatgtc gttctcccgg
      721 gtgtgttccc ccacgatcat cccctcatag acctccaccc gggggtggat gaagaacttg
      781 ccccggtcgg agagctggaa catgccatag gcggccgtga cgcccgcttc gaaggccacc
      841 agcacgcccc ggttgcggcc cgcgatctcg cccttgaact ccccgtagcc ggcgaagacg
      901 tggtgcagga tgccttcccc cttggtgtcg gtgaggaact cggtgcggaa acccagcagg
      961 cagcgcgagg ggatcatgaa ctcgatgcgg gtggtgacct ccgacagggg ggtcatgttc
     1021 ttcatctcgc ccttgcgcga ccccagtttt tccatggtca cgcccacgca gttgttgggc
     1081 acgtccacga tgagctgctc gtagggctcc tcggtcttgc
//
LOCUS       DASTJJ010000179         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_516921, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000179 DASTJJ010000000
VERSION     DASTJJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516921"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="istB"
                     /locus_tag="VFR02_02230"
     CDS             complement(<1..720)
                     /gene="istB"
                     /locus_tag="VFR02_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9869303.1"
                     /translation="MTRTHQLEASLKALALSGMLDTLEARLGQAHAGQLGHLEFLQCL
                     CEDEITRRDAAALERRLRAAHFEAATTMEDYDFSYNPKIPAAVIRDLATLRFLDAGES
                     IVLHGPVGVGKTFIAQALGHAACRRGHAVVFTKTSRLLGDLAGGHADRSWENRLRRWA
                     RPGLLILDDFAMRDFTVSQADDLYELISERANKSLIITANRAAADWYSLFPNPVVAES
                     ILDRVVNGAHHVEMPGRSYRPN"
     gene            complement(717..>2071)
                     /gene="istA"
                     /locus_tag="VFR02_02235"
     CDS             complement(717..>2071)
                     /gene="istA"
                     /locus_tag="VFR02_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9869304.1"
                     /translation="GWFPELVDARARSRTHAQIEAHRPAITEMRKTNTVATIHQRLRD
                     EAGLAVGITAFRAYMAAAFPDETARDKVTVLRPEVPPGEEAQIDYGFLGSWSDPVANR
                     VRRVWAFVMVLACSRHMFVRPVLSLDARSWVAAHVAAAVFFGGLPRRLVPDNLATGVT
                     KADIYDPKINRAYGDFAAHYSVLVDPARLRKPKDKPRVERPMPYVRDSFWRGRDWADE
                     SAMQAGALKWCMEVAGTRSHRGLEGASPLAVFNALEADALIALPLMDFELAGWSRPKV
                     GVDCHIRVGKALYSVPWRHIGTNVDVRETAELVEVFVDSKLVKTWIRIERGRQTDYSD
                     YPPEKIAFFMKTPTWCRRKANELGPNVAAVVAELTAVHVLHQLRSAQGVIGLGDKYSS
                     TRLDAACARAMAVGDVTYRTIKGILAAGTETEGLEVASVPAAPAHLHGPAGLFDEAAG
                     "
BASE COUNT          327 a          690 c          747 g          307 t
ORIGIN      
        1 gttgggccga taggaccgcc ccggcatctc cacgtggtgg gccccgttga ccacccggtc
       61 gaggatggac tcggccacca ccgggttggg gaacaacgaa taccagtccg ccgccgcccg
      121 gttggcggtg atgatcaagg acttgttcgc ccgctcggag atgagctcgt agaggtcgtc
      181 ggcctgggac acggtgaagt cccgcatggc gaagtcgtcc aggatcaaca acccgggccg
      241 ggcccaacgc cggagccggt tctcccagga ccgatcggcg tgcccgccgg ccaggtcgcc
      301 caacagccga ctggtcttgg tgaagaccac ggcgtggcct cgacgacagg cggcgtgacc
      361 gagggcctgg gcgatgaagg tcttgcccac accgacgggg ccgtgcagga cgatcgactc
      421 cccggcgtcc aagaaccgca gggtggccag gtcccggatc accgcggcgg ggatcttggg
      481 gttgtaggag aagtcgtagt cctccatggt ggtggccgcc tcgaagtggg cggcccgcag
      541 ccggcgctcg agggccgcag cgtcccggcg ggtgatctcg tcctcacaca ggcactggag
      601 gaactcgaga tgaccgagct ggccggcatg ggcctggccc agccgggcct ccagggtgtc
      661 gagcatgccg gagagggcca gggccttgag gctggcttcg agttggtggg tgcgcgtcat
      721 ccggccgcct cgtcgaagag tccggccggg ccgtgcaggt gagccggtgc ggcgggcaca
      781 gaggccactt cgaggccctc ggtctcggtg ccggcggcca ggatgccctt gatggtgcga
      841 taggtgacat cgcccacggc catggcccgg gcgcaggcgg cgtccaggcg ggtcgaggag
      901 tacttgtccc ccaagccgat cacgccctgg gccgaacgca gctggtgaag gacatgcacc
      961 gcggtcagct cggccaccac cgcggccaca ttggggccga gttcattggc ctttcgccga
     1021 caccacgtcg gggtcttcat gaagaaggcg atcttctccg gtgggtagtc cgagtagtcg
     1081 gtctggcgcc ctcgctcgat gcggatccag gtcttgacca gcttcgagtc gacgaagacc
     1141 tcgaccagct ccgctgtctc ccggacatcg acgttggtcc cgatgtggcg ccagggcacc
     1201 gagtagaggg ccttgcccac tcggatgtgg cagtccaccc cgaccttggg ccgcgaccac
     1261 ccggccagct cgaagtccat gaggggcaag gcgatgaggg cgtcggcctc caaagcgttg
     1321 aacaccgcca agggcgatgc cccttccagg ccccgatgcg atcgggtgcc ggccacctcc
     1381 atgcaccact tgagcgctcc ggcctgcatg gccgactcgt cggcccagtc ccggccccgc
     1441 cagaagctgt cccggacata gggcatgggc cgttccaccc ggggcttgtc cttgggcttt
     1501 cgcagccggg ccgggtcgac caggaccgaa taatgggcgg cgaagtcgcc ataggcccgg
     1561 ttgatcttgg ggtcatagat gtcggccttg gtcaccccgg tggccaggtt gtccggcacc
     1621 aggcgacggg gcagaccccc gaagaacacc gccgcggcca catgggccgc cacccaggag
     1681 cgggcgtcga gggacagcac cgggcggacg aacatgtgcc gggagcaggc cagcaccatg
     1741 acgaaggccc acacccgccg cacccggttg gcgacggggt cggaccacga gccgaggaag
     1801 ccatagtcga tctgggcctc ctccccggga gggacctcgg ggcgcagcac cgtcaccttg
     1861 tcccgggcgg tctcgtccgg gaaggcagcg gccatgtagg cccggaaggc ggtgatgccc
     1921 acggccaggc cggcctcgtc tctcaggcgc tggtggatgg tggccaccgt gttggtcttg
     1981 cgcatctcgg tgatggccgg gcggtgggcc tcgatctggg catgggtgcg ggaccgggcc
     2041 cgagcgtcga ccagctcggg gaaccagccc c
//
LOCUS       DASTJJ010000180         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1495715, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000180 DASTJJ010000000
VERSION     DASTJJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495715"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1132)
                     /locus_tag="VFR02_02240"
     CDS             complement(<1..>1132)
                     /locus_tag="VFR02_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869305.1"
                     /translation="YDQTSGASGVSGPPVYPSYTNGSGSSCPANGSVTAVWPIYIDGS
                     ALSAGNYSFVVQGAEDYTTCGNYQSADHFNFSVPYPPPNLSLVKIADGTSPNDGNLVL
                     FRIDYTYGNTGSSPVTITDTIPANVSLVTPTTSAISPGGSLAGSTITWILPASLPTVT
                     GEVWFLTQVNGGVAAGTAITNQASAASSATGSVTSNVAQANVGEAGFTLTKSETPNNA
                     TLNPGDIVTYGLTYQISGLNLQLYDSYDNDTTGTTNGSVKAYDGTTGYTYNTTGGTGT
                     FTVQTDPQGNHYIEAYTGTGQNGNYPALLRNSPNVSLCSGDFMVEGDMEIPKNYDTGG
                     DATMVVAFDPGTQEGYMLGMSLDNGPGNFFIQENNGPGNVSFP"
BASE COUNT          181 a          325 c          392 g          234 t
ORIGIN      
        1 cgggaagctg acattgcccg gaccgttgtt ctcctggatg aagaagttcc ccgggccgtt
       61 gtccaacgac atgcccagca tgtagccttc ctgggtgccc gggtcaaagg ccaccaccat
      121 ggtggcgtcg ccgcccgtat cgtagttctt ggggatctcc atgtccccct ctaccatgaa
      181 atccccagaa caaaggctca cgttggggct gttgcgcagc aaagccggat aattgccgtt
      241 ctggccggtt cccgtatagg cctcgatata gtggttgccc tggggatcgg tctggaccgt
      301 gaaggtcccc gtgccgccgg tcgtgttgta ggtgtagccc gtggtgccgt cataggcctt
      361 cacgctccca ttggtcgtgc ccgtggtgtc gttgtcgtag gaatcgtaca gctgcaggtt
      421 caggccgctg atctggtagg tcaggccgta ggtgacgatg tcccccgggt tgagggtggc
      481 gttgttgggc gtctcgctct tggtcagggt gaatcccgcc tcgcccacgt tggcttgggc
      541 gacgttggaa gtgaccgatc cggtggccga ggaagccgcg ctggcctggt tggtgatggc
      601 ggtccccgcg gccacgccgc cgttgacctg ggtcaggaac cagacttccc ccgtcacggt
      661 gggcaacgag gcgggcagga tccaggtgat ggtggatccc gccaggctgc cgcccgggct
      721 gatggcgctc gtggtgggcg tgaccaggct gacgttggcc gggatggtgt ccgtgatggt
      781 gaccgggctg ctgcccgtat tgccgtaggt atagtcgatg cggaacagca ccaggtttcc
      841 gtcgttgggc gaagtgccgt cggcgatctt gaccaggctc aggttgggcg ggggatatgg
      901 aacggaaaag ttgaaatggt ccgccgattg gtagttaccg caggtggtgt agtcctccgc
      961 gccctgcacc acgaagctgt aattgcccgc gctcaaggca ctgccgtcga tatagatggg
     1021 ccagaccgcc gtcaccgaac cgttcgccgg gcaggaggaa cccgaaccat tcgtatagga
     1081 gggatagacg ggcggacccg acacgcccga agcgccgctg gtctggtcgt ac
//
LOCUS       DASTJJ010000181         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1006235, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000181 DASTJJ010000000
VERSION     DASTJJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006235"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2335)
                     /locus_tag="VFR02_02245"
     CDS             complement(<1..>2335)
                     /locus_tag="VFR02_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HET9869306.1"
                     /translation="VREDYSPGGACWDYFPHDHARSRAYRWGEDGLLGLCDSKGLLCF
                     APALWNGKDPILKERLFGLSGPEGNHGEDVKECYYYLASSPTHSYMKALYKYPQAAFP
                     YAQLVEENRRRGRTQGEYELLDTGVFDGDRYFDVFVEYAKAAPEDILIRLTVVNRGPE
                     AAPLHLLPSLWLRNTWSWGRSGEHDWPRSPITRQGEALALTHPFLGAFFLTAGQPGGE
                     TLFTENETNFERLFGVPNRSPHVKDAFHEYVLKGRRDAVDPGQGTKAAFYYRAQVPAG
                     GTQTFQFRLSKGAPAPASGAGFDRAFEARRGEYEAHLAGGFAPDLPPQGRVVAEQALA
                     GLLWSKQYYYYFVQQWLEGDPAQPPVDEGRKQGRNRGWTHFGAEDVFSMPDNWEYPWF
                     AAWDLAFHTVVFAHLDPFFAKHQLVLLLREWYMHPNGQVPAYEFAFSDVNPPLHAWAA
                     WRVYKMAAPKGQRDRIFLSRVFQKLLLNFTWWVNRKDAEGNNLFSGGFLGLDNIGVFD
                     RGQLAPGEELEQADGTAWMAFYCGTLLSMALELSAEDPATEDMATKFFEHFLSIVRAI
                     NTLGGSGLWDEQDGFYYDQVLVKGTCIPMRVRSVVGIIPLFAVEILEEETLEKLPEFH
                     RRMQWFLAHKPEEAKAILCRDQGVPGRGRFLLSVPSREKLVRVLRYVLDEAEFLSPHG
                     VRMLSKYHQDHPYVLHLDGREYRIDYEPAESRTGLFGGNSNWRGPVWMPLNYLLIESL
                     ERYHHFYGDDLKVECPTGSGNLMNLKEVAGFLSQRVTG"
BASE COUNT          399 a          771 c          792 g          373 t
ORIGIN      
        1 ccggtgacgc gctgggacag gaacccggcc acctccttca ggttcatgag gttccccgag
       61 cccgtggggc attccacctt caggtcgtcc ccgtagaaat ggtggtagcg ctccagcgac
      121 tcgatgagca ggtagttcag gggcatccag accggccccc gccaattcga gttgcccccg
      181 aagagccccg tccgggactc ggcgggctcg tagtcgatgc ggtattcccg cccgtccagg
      241 tgcagcacat aggggtggtc ttggtggtat ttggacagca tgcgcacgcc gtggggcgat
      301 aggaattccg cctcatccag cacgtagcgc agcacccgca ccagtttttc ccgggacggc
      361 acggacagca ggaaccggcc ccggcccggg acgccctggt cccggcagag gatggccttg
      421 gcctcctcgg gcttatgggc caagaaccat tgcatgcgcc ggtggaactc ggggagcttt
      481 tccagggtct cctcctccag gatctccacc gcgaagaggg ggatgatgcc caccacggag
      541 cgcacccgca tggggatgca ggtgcccttg accagcacct ggtcgtaata gaagccgtcc
      601 tgctcgtccc acaggccgga cccgcccagg gtattgatgg cccgcacgat gctgaggaaa
      661 tgctcgaaga acttggtggc catgtcctcg gtggccgggt cctcggcgga aagctccagc
      721 gccatggaca gcagggtgcc gcaatagaag gccatccagg ccgtgccgtc ggcctgctcc
      781 agttcctcgc cgggcgccaa ttggccccgg tcgaagacgc cgatgttgtc caaccccaag
      841 aacccgccgg aaaagaggtt gttgccctcg gcgtccttgc ggttgaccca ccaggtgaag
      901 ttgagcagca gcttctggaa gacccgggac aggaagatcc ggtcccgttg ccccttggga
      961 gcggccatct tatagacccg ccaggccgcc caggcgtgca ggggcgggtt cacgtccgag
     1021 aaggcgaact cataggccgg cacctggccg ttggggtgca tgtaccattc gcgcagcagc
     1081 agcaccaact ggtgcttggc gaagaagggg tccaggtggg cgaagaccac ggtgtggaag
     1141 gccaggtccc aggccgcgaa ccagggatat tcccaattgt ccggcatgga aaaaacgtcc
     1201 tcggcgccga aatgggtcca gccgcggttg cggccctgct tgcggccctc gtccaccggc
     1261 ggctgggcgg ggtccccttc caaccactgc tggacgaaat agtagtagta ctgcttggac
     1321 cagaggagcc cggccagggc ctgctccgcc accacccggc cctggggcgg caggtccggg
     1381 gcgaagccgc ccgccaggtg cgcctcatat tcgccgcggc gcgcctcgaa ggcgcggtcg
     1441 aaccccgcgc ccgaggcggg ggccggggcg cccttggaga gccggaattg gaaggtctgg
     1501 gttccccccg ccgggacctg ggcgcggtaa taaaaggccg ccttggtacc ctgcccgggg
     1561 tccaccgcgt cccggcggcc cttcaggaca tactcgtgga aggcgtcctt cacgtggggc
     1621 gagcggttgg ggaccccgaa aagccgctcg aagttggtct cgttctcggt gaaaagcgtt
     1681 tccccgcccg gctgtcccgc cgtgaggaaa aaggccccca ggaaaggatg ggtcagggcc
     1741 agcgcctccc cctgccgggt gatgggcgac cggggccagt catgctcgcc cgagcggccc
     1801 cagctccagg tgttgcgcag ccagaggctg ggcaaaaggt gcaggggcgc ggcctcggga
     1861 ccccggttca ccaccgtcag ccggatgagg atgtcctccg gcgcggcctt ggcgtattcc
     1921 acgaagacgt cgaagtagcg gtccccgtcg aagacgccgg tgtccagcag ctcgtactcc
     1981 ccctgcgtgc gcccccggcg gcggttctcc tccaccagtt gggcgtaggg gaaggcggct
     2041 tgggggtact tgtagagggc cttcatatag gaatgggtgg gcgaggacgc caggtagtaa
     2101 tagcattcct tcacgtcctc gccgtggttc ccctcggggc ccgagagccc gaacaggcgt
     2161 tccttcagga tggggtcctt cccgttccag agcgccggcg cgaaacaaag caggcccttg
     2221 ctgtcgcaca ggcccagcaa cccgtcctca ccccaacggt aggcgcggct gcgggcgtgg
     2281 tcgtggggga aatagtccca gcaggccccc ccgggcgaat aatcctcccg cacgg
//
LOCUS       DASTJJ010000182         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1006333, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000182 DASTJJ010000000
VERSION     DASTJJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006333"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFR02_02250"
     CDS             <1..1127
                     /locus_tag="VFR02_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9869307.1"
                     /translation="LTVTVLEMSPQVMPPMDPEMAEALHRRLRQRGLDLRLGDGAAAF
                     EAVPEGIRVVTGSGASFTVGLVLLAIGVKPETGLARAAGLELGSTGGIRVDGEMRTSD
                     PSIFAVGDAVETRALPSGRAALIPLAGPANRQGRVAADVLAGHPRRFRGTQGTAVCGA
                     FGLTAALTGESEKSLKAAGRDYAKVYLHPGHHAAYYPDAKPILIKLLFDRGDGKVLGF
                     QAVGEEGVEKRVDVVSMAIQMGATVFDLEEAELCYAPQYGAAKDPVNVAGMIASNVMR
                     GGMELADWQALDRPGSRVVLDVRDPSEFAAGHVPGAVNIPLNELRARLGELPRDREIW
                     AYCRVGQRSYYANRVLAQHGFRVRNLPGGYLTYLSFFPPA"
     gene            1336..>1801
                     /locus_tag="VFR02_02255"
     CDS             1336..>1801
                     /locus_tag="VFR02_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9869308.1"
                     /translation="MKLQFHHFFVFAPPGAPQADRLLEAGFREGPGNRHPGQGTANRR
                     FFFDNGMLEFLWVEDRDEAHGPDAAPTRLEERSRFRETGFAPFGVATHGPLEGSVLIP
                     PFPCWSYSPPYLQPDQEIWVADNAAHPGEPMLFYAGFFPPPGRPAAGPLDHPN"
BASE COUNT          259 a          610 c          637 g          295 t
ORIGIN      
        1 gcctgacggt cacggtcctg gagatgagcc cccaggtgat gccgccgatg gatcccgaga
       61 tggccgaggc cctgcaccgt cgcctccggc agcggggcct ggacctgcgg ttgggggatg
      121 gggcggccgc ctttgaggcg gtcccggagg ggatacgggt ggtcaccggg tccggggcct
      181 ccttcaccgt ggggctggtg ctcttggcca tcggggtcaa gcccgagacc ggcctggccc
      241 gggcggcggg attggaactg gggtccaccg gcggcatccg ggtggacggg gagatgcgca
      301 ccagcgaccc ttccatcttc gccgtgggcg acgcggtgga gacccgggcc ctgccctcgg
      361 gccgggccgc gctcatcccc ctggcgggac ccgccaaccg gcagggccgg gtggccgccg
      421 acgtgctggc gggacatccc cgccggttcc ggggcaccca gggcacggcg gtctgcgggg
      481 ccttcgggct gacggccgcg ctcacgggcg agagcgagaa gtccctgaag gccgccggcc
      541 gggactacgc caaggtctac ctgcaccccg gccaccacgc ggcctattac cccgacgcca
      601 aacccatcct gatcaagctg ctcttcgacc gcggggacgg gaaggtcctg gggttccagg
      661 cggtgggcga ggaaggcgtg gagaaacggg tggacgtggt ctccatggcc atccagatgg
      721 gggcgacggt cttcgacctg gaggaggccg agctctgcta cgcgccccaa tacggcgcgg
      781 cgaaggaccc ggtgaacgtc gcgggcatga tcgcctccaa cgtgatgcgg ggcggcatgg
      841 aactggccga ctggcaggcc ctggaccggc ccggatcccg ggtcgtcctg gacgtgcggg
      901 acccttccga gttcgcggcg ggccacgtgc cgggggcggt caacatcccc ttgaacgaac
      961 tgcgcgcccg gttgggggaa ttgccgcggg accgggagat ctgggcctat tgccgggtgg
     1021 ggcagcgctc ttattacgcc aaccgggtgc tggcccagca cggcttccgg gtccggaacc
     1081 tgcccggcgg ctacctgacc tacctgagtt tcttcccgcc ggcctgagcg ggtccgggat
     1141 cggaagcccg cgtggtaacg attgccctgc gggtttggcc ggtcttccgg tcgacataaa
     1201 ataggctaaa aatatttttt cccccgatcc gctcttaccg gatgattcgg ccggcggaat
     1261 gcgctacctt atcgccttct tggacgtccc ttcttttgga aatatgaaac catggatcga
     1321 ccgggcccct caggcgtgaa actccaattc caccacttct tcgtcttcgc cccgccgggg
     1381 gccccccagg cggaccgtct gctggaggcg ggattccgcg agggcccggg caaccgccat
     1441 ccagggcagg gcaccgccaa ccgccgtttc ttcttcgaca acggcatgct ggagttcctc
     1501 tgggtcgagg accgggacga ggcgcacggt ccggacgccg cgccgacaag gctggaggaa
     1561 cgcagccggt tccgggagac gggtttcgcg cctttcggcg tggccaccca cgggcccctg
     1621 gagggatcgg tgttgatccc cccctttccc tgttggagtt acagtccgcc ctatcttcaa
     1681 cccgaccagg agatctgggt ggcggacaac gccgcccatc ccggggagcc gatgctcttt
     1741 tacgccggtt tttttccccc gcctgggcgc ccggcggcgg gccccctgga ccacccgaac
     1801 g
//
LOCUS       DASTJJ010000183         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1617818, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000183 DASTJJ010000000
VERSION     DASTJJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617818"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFR02_02260"
     CDS             <1..960
                     /locus_tag="VFR02_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9869309.1"
                     /translation="VGGAYFDLCRLSFDLFPEWIERLRAEGGLDCEYLESGSLDLAYD
                     DKDGEALAKLEKKMAAQGLAGRRLTGAQAREKEPALSPDVQSAFFLGSTRQVRPPRLT
                     RALLAALQKQGAELRELERVKGFLVRGRAITGVETHRGLLEADAVVLATGAWTGLWGE
                     KFKRVIPVKPVRGQVVLFQTPPGFLKHILFSPLAYLVPRRDGRLYVGSTLEEVGFDKS
                     TTSSALERLKRGARRALPGLEDLLLEDSWAGLRPGSPDGWPFLGKFPEWEGLFIAAGH
                     YTHGFLLSAATGRLLAQAILGETPEMDLGPFALGRRPHASVGL"
     gene            1052..2014
                     /gene="rfaD"
                     /locus_tag="VFR02_02265"
     CDS             1052..2014
                     /gene="rfaD"
                     /locus_tag="VFR02_02265"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HET9869310.1"
                     /translation="MSILLTGAYGFIGARLAHALLAQGRADLLLVDKKDYAASRSCAK
                     GLEKVPFLDRDRLIAELPGLAGVSAVIHLGACTDTGNHDEAYMWKMNTDYTKALWQWC
                     AARQVPLVYASSGATYGRGDEGYSDDHADIPRYKPLNVYGRSKHEFDLWALAQPAAPP
                     RWQGLKFFNVYGMDENHKGRMASPVFHGFHEVQKTGQQTLFRSHKEGVADGEQKRDFI
                     FVDDIVKLCLFCLDRAPASGIYNCGTGRARTFLDVSRALFSALGREQKIHWVDTPEKF
                     RAGYQYFTQADMGKMRRAGYTEPFLSVEEGVSRYAKWLSDQFPA"
     gene            2052..>2677
                     /locus_tag="VFR02_02270"
     CDS             2052..>2677
                     /locus_tag="VFR02_02270"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9869311.1"
                     /translation="MALYPVVLQTQIPGVPPPKRGKVRDIYDLGDKLLIVTTDRISAF
                     DVILPNGIPDKGVVLNQISLYWFKQMEALIPNHILSSDLAGLPEPFQKAKDQLAHRSV
                     VVKKAKPLAAECIVRGYLTGSGLKEYQKTGKVCGLQLPAGLVESSKIAHPIFTPSTKA
                     EEGHDQNISFEEMAVLLKDGALAEKVKDVSLAIYQRGRDLADTKGIIIA"
BASE COUNT          480 a          891 c          851 g          455 t
ORIGIN      
        1 gtgggcgggg cctatttcga cctttgccgg ctctccttcg acctcttccc cgaatggatc
       61 gagcggctgc gggccgaagg cggactggat tgcgagtacc tggagtcggg aagcctggac
      121 ctggcctatg acgacaagga cggggaagcc ctggcgaagc tggagaaaaa aatggccgcc
      181 caggggctgg cgggccgccg cctgaccggc gcccaagcgc gggagaagga gccggccctc
      241 tcgcccgatg tccagtcggc cttcttcctg ggatccaccc ggcaggtccg cccgccccgc
      301 ctgacccggg ccctcttggc cgcccttcaa aaacaagggg ccgagttgcg ggagttggag
      361 cgggtgaagg ggttcctggt ccggggccgc gccatcacgg gcgtggagac ccaccggggc
      421 ctgttggagg cggacgcggt ggtcttggcc acgggggcct ggaccggttt gtggggcgag
      481 aagttcaagc gggtgatccc ggtcaaaccc gtgcgcggcc aggtcgtgtt gttccagacc
      541 ccgccgggtt ttctaaagca catcctgttc tccccactgg cctatttggt cccgcggcgc
      601 gacggccgtc tttacgtcgg gagcaccctg gaagaagtcg ggttcgacaa aagcacgacc
      661 tcctcggccc tggagcgctt gaagcgcggc gcccggcggg ccctgcccgg gctggaagac
      721 cttttgttgg aggacagctg ggcgggattg aggccgggtt cccccgacgg ctggcctttc
      781 ctggggaagt tccccgaatg ggaagggctc ttcatcgcgg cgggccatta cacccacggc
      841 tttttgcttt ccgccgccac cggccgcctg ctggcccagg ccatcctcgg ggaaacgccc
      901 gaaatggact tggggccctt cgccctgggc cgccggcccc acgcctccgt cgggctctaa
      961 ggcggatttc aggtcccccc ggtccgccgc tgcctatata atgggccctc ttccaggggc
     1021 cgcaggccca tcccaaacgc aaggaaggtc catgtccatc ctcctcaccg gagcctacgg
     1081 tttcatcgga gcccgcttgg cccacgccct gctggcgcaa ggccgcgccg acctgttgct
     1141 ggtggataaa aaggactatg cggcctcccg cagctgcgcc aagggactgg aaaaggtccc
     1201 cttcctggac cgcgaccgct tgatcgccga actgcccggg cttgccggcg tctcggccgt
     1261 gatccacctg ggggcctgca cggacacggg caaccacgac gaagcctaca tgtggaagat
     1321 gaacaccgac tacaccaagg ccctctggca atggtgcgcc gcgcgccagg tcccgttggt
     1381 gtacgcctcc agcggggcca cctatggccg gggcgacgag ggttattcgg acgaccacgc
     1441 cgacatcccc cgctataaac ccctcaacgt gtacggccga agcaagcacg agttcgacct
     1501 ctgggccttg gcgcaacccg ccgcgccgcc ccgctggcag ggcctgaagt tcttcaacgt
     1561 ctacggcatg gacgagaacc acaagggccg catggcctcg ccggtcttcc atggcttcca
     1621 cgaggtccaa aagaccggcc agcagaccct tttccgttcc cataaggaag gcgtggccga
     1681 cggggaacaa aagcgggatt tcatcttcgt ggacgacatc gtgaaattgt gccttttctg
     1741 cctcgaccgg gcgccggcct ccggcatcta caattgcggc acgggccggg cccggacctt
     1801 cctggacgtt tcccgggcgc tcttttcggc gttgggaagg gaacagaaga tccattgggt
     1861 ggacacgccg gaaaagttcc gcgcgggcta ccagtatttc acccaagccg acatgggcaa
     1921 gatgcgccgc gcgggctaca ccgagccctt cttgagcgtg gaagagggcg tgtcgcgtta
     1981 cgcgaagtgg ctgtcggacc aattcccggc ctgagggccg gaaaaaaacg gaagttccaa
     2041 ggaggcgatg gatggctttg tatcccgtgg tgctccagac ccaaattccc ggcgtgccgc
     2101 cccccaagcg gggcaaggta cgcgacatct atgacctggg ggacaagctc ctcatcgtca
     2161 ccacggaccg catctcggcc ttcgacgtca tcctgcccaa cggcatcccg gacaagggcg
     2221 tggtgctcaa ccagatctcc ctctactggt tcaagcagat ggaagccctc atcccgaacc
     2281 acatcctttc atccgacttg gccggcctgc ccgagccctt ccaaaaggcc aaggaccagt
     2341 tggcccaccg ctccgtggtg gtgaagaagg ccaagccctt ggccgccgaa tgcatcgtgc
     2401 ggggttattt gaccggatcg ggcctgaagg aataccaaaa gaccggcaag gtctgcggcc
     2461 tccaactgcc cgcggggctg gtggagtcct ccaagatcgc ccatcccatc ttcaccccct
     2521 ccaccaaggc cgaggaaggc cacgaccaga acatctcctt cgaggagatg gccgtccttt
     2581 tgaaggacgg ggcgttggcc gaaaaggtca aggatgtgag cctggccatc taccagcggg
     2641 gccgcgacct ggccgacacc aaggggatca tcatcgc
//
LOCUS       DASTJJ010000184         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1129128, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000184 DASTJJ010000000
VERSION     DASTJJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129128"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1041
                     /locus_tag="VFR02_02275"
     CDS             <1..>1041
                     /locus_tag="VFR02_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9869312.1"
                     /translation="AIVVRENIWRHIEGGEDPKKAAVSGALEVMLAVVATSSVVISVF
                     FPIAFLKGTIGQFFREFGLSVCFAMAVSLFESLVMGPMLSAYLGKKGTKDPLGYWRQK
                     VRASAWFNPWQAFHRVMFALTVVTTRFARFQDILEERYVRLIDWCLEHRLWVVLGAAG
                     IFLASVIGLGPHIKSTFLPSADVGEFVVQLKAPPGTSLEAMRDETVKVDDLIRKHPEV
                     VLVSDTIGGTDSTGVAQQDANTARIYVKLLHYDKRKKSTAQIKDEVRDELEPYQKALN
                     PQVADQSGFGDMAPFNLNLIGQDYSVLVPLAEQVADKIRGIHGLADVQSTYDGGKPEF
                     QAVLDPEKLRLF"
BASE COUNT          175 a          341 c          336 g          189 t
ORIGIN      
        1 acgccatcgt ggtgcgcgag aacatctggc ggcacatcga gggcggcgag gaccccaaga
       61 aggccgccgt cagcggggcc ctggaggtca tgctggcggt cgtggccact tcctcggtgg
      121 tcatctcggt ctttttcccc atcgccttcc tcaagggcac catcggccag ttcttccggg
      181 aattcgggtt gtcggtgtgt ttcgccatgg cggtgtcgct ctttgaatcg ctggtcatgg
      241 ggcccatgct gtccgcctac ctgggcaaga aggggaccaa ggatccgctg ggctattggc
      301 gccaaaaggt ccgggcttcc gcttggttca atccctggca ggccttccac cgggtgatgt
      361 tcgccctgac cgtcgtgacc acccgcttcg cgcggttcca ggacatcctg gaggagcgtt
      421 atgtgcgcct gatcgactgg tgcctggaac accgcctctg ggtggtgctg ggcgcggccg
      481 ggatcttttt ggcctcggtc atcggcctgg ggccccacat caagtccacc ttcctgccct
      541 cggcggacgt gggcgagttc gtggtgcagc tcaaggcccc gcccgggacc tccctggagg
      601 ccatgcggga tgagaccgtg aaggtggacg acctcatccg caaacacccc gaggtggtgc
      661 tggtgtccga caccatcggc ggcaccgact ccaccggtgt ggcccagcag gacgccaaca
      721 cggcgcgcat ctacgtgaag ctgctgcatt acgacaagcg caaaaaatcc accgcccaga
      781 tcaaggacga ggtgcgtgac gaactcgagc cctaccagaa ggccctgaac ccccaggtgg
      841 ccgaccaaag cggcttcggc gacatggcgc ccttcaacct gaacctcatc ggccaggact
      901 attcggtgct ggtgcccctg gccgaacagg tggccgacaa gatccggggc atccacggcc
      961 tggcggacgt gcagtccacc tatgacgggg gcaagcccga gttccaggcg gtgctggacc
     1021 ccgagaagct gcgcctcttc g
//
LOCUS       DASTJJ010000185         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_517223, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000185 DASTJJ010000000
VERSION     DASTJJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517223"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFR02_02280"
     CDS             <1..410
                     /locus_tag="VFR02_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9869313.1"
                     /translation="IEKIAGGFLDFDVLVATPDIMRETGKLGKVLGPKGLMPNPKAGT
                     VTPNVGQAVKEIKAGKIEYRVDDQGICHAGVGKLQFSEQQIVENAQTLIRTLVAVKPA
                     SAKGTYLMSAHLTSSMGPGIKLETAEYLKTAKA"
     gene            641..1195
                     /gene="rplJ"
                     /locus_tag="VFR02_02285"
     CDS             641..1195
                     /gene="rplJ"
                     /locus_tag="VFR02_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET9869314.1"
                     /translation="MIFLADTKREPKQEKIEAVQAFYDRLQQAKAAVVTGYTGLTVEE
                     VTQLRAQLFHAKVEYHVVKNNLARIAFNKHNITVLDDLLKGPTAVALAMDDAVAPARV
                     LSKFAKDHDKLSIKGGWLDGRLITDKDIKALANLPSKEVLLAKMLGSMKSPISGIVQV
                     LAGPARKLVYALDAVAKAKAKTAA"
     gene            1253..1633
                     /gene="rplL"
                     /locus_tag="VFR02_02290"
     CDS             1253..1633
                     /gene="rplL"
                     /locus_tag="VFR02_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9869315.1"
                     /translation="MPTLTKDEMITAIEGMTVLELNDLVKALEEKFGVSAAAPVAVAA
                     AGAGAGAAAAEEKTGFDVILKAAGGNKITVIKVVREVTGLGLKEAKDLVDGAPKPIKT
                     GVPKAEADGIVEKFKESGAEIEVK"
BASE COUNT          366 a          491 c          545 g          253 t
ORIGIN      
        1 tcatcgaaaa gatcgcgggc gggttcctgg acttcgacgt cctggtggcc accccggaca
       61 tcatgcgcga gacgggcaag ctggggaagg tgttgggccc caagggtctc atgcccaacc
      121 ccaaggcggg caccgtcacc cccaacgtgg gacaggccgt gaaggagatc aaggccggga
      181 agatcgagta ccgcgtggac gaccaaggca tctgccacgc cggcgtgggc aagctgcagt
      241 tctccgagca gcagatcgtg gagaacgccc agacgctcat ccgcaccctg gtggcggtga
      301 agccggcctc ggccaagggg acctacctga tgtccgccca cctgacttcc agcatgggtc
      361 cgggcatcaa gttggagacc gccgagtacc tgaagacggc caaggcctaa ggcccgccgg
      421 gaagaacaac tgaatcagac gtcccgcccc tccgggggcg gggtcaagga tgtccaagac
      481 agcgggtggc ggggcgaaga gcccggcctt aatgttcccg ccgagacggt ccgctttgaa
      541 gagttccata cccttcatcc ggtcgtttcg gatgaagggt tttcttttgg cctggaaagg
      601 ccccccttta tcccgaagtg caatccaagc aagaagggtg gtgatctttt tggcggatac
      661 gaaacgcgaa cctaaacagg agaagatcga ggccgtgcag gccttttatg accggctcca
      721 acaggcgaaa gcggcggtgg tgaccggtta taccggcctg accgtggagg aagtgaccca
      781 attgagggcc caacttttcc acgcgaaggt cgagtaccac gtggtcaaga acaacctcgc
      841 ccggatcgcc ttcaacaagc acaacatcac ggtgttggac gacttgttga agggccccac
      901 ggccgtggct ttggccatgg acgacgcggt ggcccccgcg cgggtgcttt ccaaattcgc
      961 aaaggaccat gacaagttgt ccatcaaggg cggctggctc gacggccggc tgatcacgga
     1021 caaggacatc aaggccctgg cgaacctgcc gagcaaggaa gtgttgctgg ccaagatgct
     1081 gggcagcatg aagtcgccca tcagcggcat cgtgcaggtg ctggccggac cggcccgcaa
     1141 gttggtctac gcgctggacg ccgtggcgaa agccaaggcc aagacggccg cgtaaaaccc
     1201 gtaaacccga agactcaccg gggctttgcc ccatgaccta gaggagaatg aaatgcctac
     1261 tttgacgaaa gacgagatga tcacggccat cgaagggatg accgtcctgg aattgaacga
     1321 tctggtgaag gccttggaag agaagttcgg cgtgtccgcc gcggcccccg tggccgtcgc
     1381 cgccgccggt gcgggcgcag gcgccgccgc cgccgaggag aagaccggtt tcgacgtgat
     1441 cctgaaagcc gcgggcggca acaagatcac cgtcatcaag gtggtccgcg aggtcaccgg
     1501 cctgggcttg aaggaagcca aggacctggt ggacggggcc cccaagccga tcaagaccgg
     1561 cgtgcccaag gccgaggccg acggcatcgt cgagaagttc aaggagtccg gcgccgagat
     1621 cgaagtgaag taaaagggtc ccgccggggc ccaaa
//
LOCUS       DASTJJ010000186         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1129148, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000186 DASTJJ010000000
VERSION     DASTJJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129148"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFR02_02295"
     CDS             <1..362
                     /locus_tag="VFR02_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET9869316.1"
                     /translation="WKRHEALARATRAGIEAIGLKPFAPTAPSPALTSVLAPEGIDSG
                     AVNKTFRDGFGVSIAGGQGKVKGKVFRLGHLGYVDGSDVILGMAVCEMTLTKLGHKVP
                     LGAGVKAVEEIVLAENL"
     gene            528..>1215
                     /locus_tag="VFR02_02300"
     CDS             528..>1215
                     /locus_tag="VFR02_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET9869317.1"
                     /translation="MFKILITDKLSDLGMEVFRKEKEIQADEVFGKTPEELKAILPQY
                     DAWVIRSGTTATAELIEAAAKMKVIGRAGVGVDNVDLPAATKRGIIVMNTPDGNTIST
                     SEHTVAMLMAMARQIPQAQQSLKEKKWERSKFIGCELNEKVLGVVGLGRIGSNVARKA
                     IGLGMRVIAYDPFIDPAKAKGHEFEIVTLDDVIKRSDFITVHTPLTAETKGLLGRKQI
                     AAMKKGVRLVN"
BASE COUNT          247 a          369 c          403 g          196 t
ORIGIN      
        1 tctggaagcg gcacgaggcc ctggcgcgcg ccacccgggc ggggatcgag gccatcgggc
       61 tcaagccctt cgcccccacg gccccctcgc cggccctgac ctccgtgttg gcgcccgagg
      121 gcatcgacag cggcgcggtc aacaagacct tccgggacgg ctttggggtc agcatcgccg
      181 ggggccaggg caaggtgaag ggcaaggtct tccgactggg ccacctgggc tacgtggacg
      241 gttcggacgt gatcctgggg atggcggtgt gcgagatgac gctcaccaag ctggggcaca
      301 aggtccccct gggcgccggc gtgaaggccg tcgaggagat cgtgctggcc gagaaccttt
      361 aaggcggccg gcgaaccttt tcaccgcaaa gacgccaagt gcgccaagtg aggcgaagat
      421 cttgaggatg tgtttctggg ttccccgtca gggcctgttt ttcttggcga acttggcgtc
      481 ttggcggtga atgttctttg attttcaaat gaacggatgg gggaaaaatg ttcaagatcc
      541 tgatcaccga taagctgtcc gacctgggca tggaggtctt ccgcaaggaa aaagagatcc
      601 aggccgacga ggtgttcggc aagacgcccg aggaactcaa ggccatcctg ccccaatacg
      661 acgcctgggt catccgcagc ggcaccacgg ccacggccga gctcattgaa gcggccgcca
      721 agatgaaggt catcggccgc gccggcgtgg gcgtggacaa cgtggacctg cccgccgcca
      781 ccaagcgggg catcatcgtg atgaacacgc ccgacggcaa caccatctcc acctccgagc
      841 acacggtggc catgctcatg gccatggccc ggcagatccc ccaggcccaa cagtccctga
      901 aggaaaagaa atgggaacgc agcaagttca tcggctgcga gctcaacgag aaggtgctgg
      961 gcgtggtggg cctgggccgc atcggcagca acgtggcccg caaggccatc ggcctgggca
     1021 tgcgggtgat cgcctatgac cccttcatcg acccggccaa ggccaagggc catgagttcg
     1081 agatcgtgac cctggacgac gtgatcaaaa ggtccgattt catcaccgtg cacacgccat
     1141 tgaccgccga gaccaagggc ctgctgggca ggaaacagat cgccgccatg aagaagggtg
     1201 tgcggttggt caatt
//
LOCUS       DASTJJ010000187         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_150220, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000187 DASTJJ010000000
VERSION     DASTJJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150220"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFR02_02305"
     CDS             <1..225
                     /locus_tag="VFR02_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869318.1"
                     /translation="TINFDDRPPSMHEALTYSTSHPYGACCCGLWVRAWDDPRLPFEF
                     QNDRYVRRWLALGYFFHPGEVAYLYPAYAR"
     gene            362..535
                     /locus_tag="VFR02_02310"
     CDS             362..535
                     /locus_tag="VFR02_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869319.1"
                     /translation="MLKLDQYAEGILYQCDQCQFVGELEIHIVEDEGFFICWNCLNEL
                     NHRSRKAAASPSR"
     gene            complement(543..2144)
                     /locus_tag="VFR02_02315"
     CDS             complement(543..2144)
                     /locus_tag="VFR02_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET9869320.1"
                     /translation="MADPISPNEIKNAVAKRRTFAIISHPDAGKTTLTEKLLLYSGMI
                     QTAGMVRGRKGGKVAVSDWMGMEQERGISITASAMQFPYKNAVINVLDTPGHQDFSED
                     TYRTLTAADCAIMVIDAAKGVETQTRKLFEVCRLRGIPVMTFINKMDLPGRDPLDLMQ
                     EVEQVLGIHASPINWPVGSGREFVGVADRRAKELLLFSKVSVGGAAKAAVERVPLEGP
                     ELARHVTGELLEKLNHDLELLEVAGNPYERGEFLKGKVTPVFFASALTNFGVEPFFDA
                     FVEMAPPPGPRLADADGGGEVLIDPIETPFSAYVFKLQANMNPKHRDSTAFLRVCSGR
                     FERDLVVKHHRLGKDLRLSRPHTMLAADRNNLDLAYPGDIVGVINPGVFAIGDTLSLT
                     GGFSFKPLPSFQPEIFCRVRPKDVLKRKPFDKGVQQMTDEGAVQMLKSWDGLDFYVAA
                     VGRLQFDVLQYRLKGEYGVDTELELLPYTCSAWLVGDPEKFDPPVQALKTRDRLDRPV
                     ILFKSPWDKDYTARKCPDHQFLDMA"
     gene            2273..>2543
                     /locus_tag="VFR02_02320"
     CDS             2273..>2543
                     /locus_tag="VFR02_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HET9869321.1"
                     /translation="MRDTFCLAWDQAHGAKLVDFHGWNMPVSYAKGILFEHEATRNGA
                     GLFNISHMGRLRLHGPQAYKFCQYAFTNDLSKALIGTALYGFLLND"
BASE COUNT          434 a          840 c          810 g          459 t
ORIGIN      
        1 accatcaatt tcgacgaccg accgccctcc atgcatgagg cgctcaccta ttcgaccagc
       61 catccctacg gcgcctgttg ttgcgggctt tgggtgaggg cctgggacga cccccggctg
      121 ccttttgagt tccagaacga ccgttatgtc cgccgttggc tggccctggg ctatttcttc
      181 catccggggg aagtggccta tctttacccc gcctacgccc gctgaggggc cgctttaacc
      241 gcttgttcgg accgccgtaa caagggctta accgcccgac ggcaacctag ctgggcacgc
      301 ccacccgacc cccttccccc tcccacccca cgctgaagca cttttccgaa aggaggggcc
      361 catgttgaag ctcgaccaat acgccgaagg catcctgtac cagtgcgacc aatgtcagtt
      421 cgtgggggaa ttggagatcc acatcgtgga ggatgaaggt tttttcatct gctggaactg
      481 cttgaacgag ttgaaccacc gcagccgcaa ggcggcggca tccccttccc ggtgacccgg
      541 ccttaggcca tgtccaagaa ttggtggtcg ggacatttgc gcgccgtata gtccttgtcc
      601 caggggctct tgaacaaaat gaccgggcgg tccagccggt cccgggtctt caaggcctgc
      661 acgggcgggt cgaatttttc cgggtcgccc accagccagg cgctgcaggt gtagggcaaa
      721 agctccagct cggtgtccac cccgtattcc cccttcagcc ggtactgcag cacgtcgaac
      781 tgcaggcggc ccaccgcggc cacgtagaag tccaacccgt cccagctctt gagcatctgc
      841 accgcgccct cgtcggtcat ctgctgcacg cccttgtcga agggcttgcg cttcagcacg
      901 tccttggggc gcacccggca gaagatctcg ggctggaacg agggcagggg cttgaaggaa
      961 aagccgccgg tcaaggacag ggtgtccccg atggcgaaga cgccggggtt gatcacgccc
     1021 acgatgtcgc ccgggtaggc caggtccagg ttgttgcggt ccgccgccag catggtgtgg
     1081 gggcgcgaca ggcgcaggtc cttgcccaac cggtggtgct tcaccaccag gtcccgctcg
     1141 aaacggcccg aacagacccg caggaaggcc gtggaatccc ggtgtttggg gttcatgttg
     1201 gcctgcagct tgaagacgta ggcgctgaag ggggtctcga tggggtcgat gaggacctcg
     1261 ccgccgccgt cggcgtccgc cagccggggc ccggggggcg gggccatttc cacgaaggcg
     1321 tcgaagaagg gctccacgcc gaaattggtg agggccgagg cgaagaagac cggcgtgacc
     1381 ttgcccttga ggaattcccc ccgctcgtag gggttgccgg ccacctccag cagctccagg
     1441 tcgtggttca atttttccaa aagctcgccc gtcacgtgcc gggccagctc ggggccctcc
     1501 aggggcaccc gctccacggc cgccttggcg gcgccgccca cggacacctt ggaaaagagc
     1561 agcagctcct tcgcgcggcg gtcggccacg cccacgaatt cccggccgga cccgacgggc
     1621 caattgatgg gcgaggcatg gatgcccagg acctgctcca cctcctgcat caggtccagg
     1681 gggtcccggc cgggcaggtc catcttgttg atgaaggtca tgaccgggat gcccctgagg
     1741 cggcagacct cgaagagctt gcgggtctgg gtctccacgc ccttggcggc gtcgatgacc
     1801 atgatggcgc agtcggcggc cgtgagggtg cggtaggtgt cctcggagaa gtcctggtgg
     1861 cccggggtgt ccagcacgtt gatgacggcg ttcttgtagg ggaactgcat ggcggaggcc
     1921 gtgatggaaa tgccccgctc ctgctccatg cccatccagt cggacacggc caccttgccg
     1981 cccttgcggc cccgcaccat gccggcggtc tggatcatcc ccgaatagag cagcagcttc
     2041 tcggtcaggg tggtcttgcc cgcgtccggg tggctgatga tggcgaaggt gcgccgtttg
     2101 gccacggcgt tcttgatctc gttgggggaa atggggtcgg ccataggccg cacatgataa
     2161 caggccccgc cgggtccttg aagccccccg ggccccgaac ggattggtat tgggcgcccc
     2221 cttgggctaa agtagggccc ggaccgcagc cattccttcc aaaagggagc ccatgagaga
     2281 caccttctgc ctggcctggg accaagccca cggcgccaaa ttggtcgatt tccacggttg
     2341 gaacatgccg gtgagctacg ccaagggcat cctcttcgag cacgaggcca cccggaacgg
     2401 cgcggggctc ttcaacatct cccacatggg gcggctgcgc ctccacgggc cccaggccta
     2461 taaattttgc caatacgcct tcaccaacga cctgtccaag gccctcatcg gcacggccct
     2521 ctacgggttc ctgctcaacg acc
//
LOCUS       DASTJJ010000188         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1496317, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000188 DASTJJ010000000
VERSION     DASTJJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496317"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFR02_02325"
     CDS             complement(<1..512)
                     /locus_tag="VFR02_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869322.1"
                     /translation="MAPVKPSPTAVVPTATPTPQPEVEEFAYTLLENQKPSLWTMDTD
                     GTNAVRLSPPGVSCWYPLWSPSGKKLAYLSDQADGKINLFILDKGGKTPQQVTFYDDM
                     SLPAPGTLKAPLSWSPKSDQVAYIYHKQVWVVDLTAQTTRSVFTPGDPSYSISDIEWA
                     PYRDNKSIAF"
     gene            complement(833..>1329)
                     /locus_tag="VFR02_02330"
     CDS             complement(833..>1329)
                     /locus_tag="VFR02_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869323.1"
                     /translation="IPLAAPSGGRLDLALRLPLSPDPWFPALGNALVVLVLTLLLLRT
                     APASGSARVPAADPPAASPVPLPPGPADPDSQAVLDVDEGYRLGSVSPRAALLLRQDR
                     EALLGRHLLDLAPHPALLEIMRSGLEGSVPDPFPPLPLAARVLPETNRWKIRLEPTQN
                     GQKR"
BASE COUNT          254 a          340 c          495 g          240 t
ORIGIN      
        1 aagaaggcga tggatttatt gtcccggtag ggcgcccatt cgatgtccga gatgctgtag
       61 gagggatcgc cgggggtgaa gaccgaacgg gtggtttggg cggtcaggtc cacgacccag
      121 acctgcttgt ggtagatgta ggcgacttga tcgcttttcg gcgaccagct gaggggggcc
      181 ttcagggtgc ccggcgcggg cagggacatg tcgtcgtaaa aggtcacctg ctggggcgtt
      241 ttgccgccct tgtccaggat gaacaggttg atcttgccgt ccgcttggtc cgacagatag
      301 gccagctttt tgccgctggg ggaccagagg ggataccagc aactgacgcc cggggggctc
      361 aggcgcacgg cgttggtgcc gtccgtatcc atggtccata gggagggttt ttggttttcc
      421 agcagcgtgt aggcgaattc ctccacttcg ggttgggggg tgggggtcgc ggtcggaaca
      481 acggcggtgg gggaaggctt caccggggcc atggaaattt ccatttggcc ttgatcctgg
      541 ggatggatgt ccgtcgcggc aggggcgctg gctgtgggcg gggccgtcac ggcgctggtg
      601 gccgctgggg gcctggactc gggaatcctt ttaaagaagg gaagggccct ttttccgcaa
      661 ccggggatgt tcactacgca tccggatgaa acaagcagta aaaccaaaag catcgccgaa
      721 aaaatggaga gacttttaaa ggtttcgcgc aagcggggta cccttggatg acgtgatgaa
      781 aaccttctat ttgcaggcag tttatggcct gatcaaataa aagtcaacaa aatcaacgtt
      841 tttggccatt ttgggtcggc tccaatcgga tcttccagcg gttggtttcc ggcaagaccc
      901 gggccgcgag cggaaggggc gggaaagggt cgggcacgct tccctccagt ccactgcgca
      961 tgatctccaa aagggctgga tggggggcta ggtccaacag atgccggccc agcaaggcct
     1021 cccggtcttg ccggagcaga agggccgccc gggggctgac ggaacccaat cggtatccct
     1081 cgtccacgtc caggacggcc tgggaatcgg ggtcggcggg tcctggggga agggggaccg
     1141 gggaggcggc gggtggatcc gccgcgggga cccgggcgga cccggaagcc ggggcggtcc
     1201 taagcaagag cagggtcagg accaagacga ccagagcgtt acccagggcc gggaaccagg
     1261 ggtcgggcga aaggggaagg cgcagggcca ggtccagccg cccaccggag ggtgcggcca
     1321 ggggaatgg
//
LOCUS       DASTJJ010000189         1488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1374007, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000189 DASTJJ010000000
VERSION     DASTJJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374007"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VFR02_02335"
     CDS             <1..1488
                     /locus_tag="VFR02_02335"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET9869324.1"
                     /translation="DTHPTIAIVELMRILLDEEGLDWESAWEITQKVFAYTNHTLLPE
                     ALEKWSVGLFGRLFPRHLQIIQEINVRFLREVANCFPGDVDKLRQMSIFEEGHEPHIR
                     MAFLAIVGSHSVNGVSALHTELLKSTVVPDFYKLYPERFNNKTNGITPRRWLRKCNTG
                     LSDLITSRIGDGWVTDLSKLKKLEKYADDRKFQAEWREVKRANKERLAHLVAEEQRLA
                     IDVNSMFDVQVKRIHEYKRQLLNALHVISLYLRIKADPAAPFTPRTVFFGGKAAPGYF
                     KAKLIIKFINAIADMVNRDPQIKGKLMVVFLPNYRVSLAEKVIPATDLSEQISTAGKE
                     ASGTGNMKFALNGALTIGTLDGANVEMQEEVGDDNIFIFGLKAEEVAKVKREGYRPRD
                     YYEKSPGLKSVVDLIGSGFFSPENPDLFKPIRHSLLEEDEYLLLADFDAYMECQQRVA
                     ELDRDQDEWTRKAILNVARMGKFSSDRTIREYCDEIWDIKPVKIQ"
BASE COUNT          314 a          487 c          435 g          252 t
ORIGIN      
        1 gacacccacc ccaccatcgc catcgtggag ctgatgcgca tcctgttgga cgaggagggc
       61 ctggactggg aatccgcttg ggagatcacc caaaaggtct tcgcctacac caaccacacc
      121 ctgttgcccg aggcgctgga aaagtggtct gtgggccttt tcggacggct tttcccccgg
      181 cacctgcaga tcatccaaga gatcaacgtg cgtttcctgc gggaggtggc caactgcttc
      241 cccggggacg tggacaagct gcgccagatg tccatctttg aggagggaca cgagccccac
      301 atccgcatgg ctttcctggc catcgtgggc agccattcgg tgaacggcgt ttcggcgctg
      361 cacaccgaac tcttgaagtc caccgtggtg ccggacttct ataaactgta tcccgagcgt
      421 ttcaacaaca agaccaacgg catcacgccg cgccgctggc tgcgcaaatg caatacgggg
      481 ctttccgacc tgatcacctc ccgcatcggg gacggctggg tcaccgacct gtccaagctc
      541 aagaagctgg aaaagtacgc ggacgaccgc aagttccagg ccgaatggcg cgaggtcaag
      601 cgggccaaca aggaacgcct ggcccacctg gtggccgagg aacagcgctt ggccatcgac
      661 gtcaactcca tgttcgacgt ccaggtcaag cgcatccacg agtacaagcg ccagctcctg
      721 aacgccctgc acgtcatttc gctgtacctg cgcatcaagg ccgatcccgc ggcgcccttc
      781 acgccccgca cggtcttttt cggcggcaag gcggccccgg gctatttcaa ggccaagctc
      841 atcatcaagt tcatcaatgc catcgccgac atggtgaacc gagaccccca gatcaagggc
      901 aagctgatgg tggtcttcct gcccaactac cgggtatcgc tggccgagaa ggtcatcccg
      961 gccaccgacc tgtccgaaca gatctccacc gccggcaagg aggcctcggg cacgggcaac
     1021 atgaagttcg ccttgaacgg ggccctcacc atcgggaccc tggacggcgc caacgtggag
     1081 atgcaggagg aagtggggga cgacaacatc ttcatcttcg ggctcaaggc cgaggaagtg
     1141 gccaaggtca agcgggaggg ctaccggccc cgcgactatt acgagaaatc gcccggcctc
     1201 aagtcggtgg tggacctcat cggcagcggc ttcttctcgc cggagaaccc cgacctcttc
     1261 aagccgatcc gtcactccct tttggaggag gacgagtacc tgctcctggc ggacttcgac
     1321 gcctacatgg aatgccagca gcgcgtggcc gagctcgacc gcgaccagga cgagtggacc
     1381 cgcaaggcca tcctgaacgt ggcccgcatg ggcaagttct ccagcgaccg caccatccgc
     1441 gaatactgcg acgagatctg ggacatcaag ccggtgaaga tccagtag
//
LOCUS       DASTJJ010000190         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1741040, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000190 DASTJJ010000000
VERSION     DASTJJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741040"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFR02_02340"
     CDS             <1..608
                     /locus_tag="VFR02_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869325.1"
                     /translation="FDDSNPAAAPTVVLSSLFFPTGLRRDLAGNFYVAESDNGGVAQF
                     LDLYDPTLTTRENQCALPDVWGAAVNETGEVYVSGLNSFAVTEVQGCALEPTVTPTPA
                     YQGASPPGAGGAFVYPSPVRGDRASVAYRLPAPAGVRLTVWNEAGEKITEVTDHRGAG
                     VQATAFSVAGWSPGVYFYGLTLSYDSGGVEKPPLGKFAVIH"
     gene            747..>1781
                     /locus_tag="VFR02_02345"
     CDS             747..>1781
                     /locus_tag="VFR02_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869326.1"
                     /translation="MAAAQTPPLGPLAQVRSMGITLPVYTCGATPVPGGTCCPGLTSL
                     DGFVASGGMALDPARHRLYAADFENNRVQVFDSQSLARVGLISALGSSGALNLPVDAA
                     LDADGNLYVADLGNEAVEKFDANLHYVASIAAGKGLSIVGVWAEGDSVYFSTLQNYVY
                     RYTQGVSGYSASATFGEPGQLNHPNQIVKSGNWLYVTDTYNDRLVKFDVDHPGPVPTQ
                     VVGCLLIPTGLRPDATGKWLLEESDNGGYPAYVDTFSHDFSLLKSRCVFPDTWSAVQD
                     PATGRTFVSGMNSLSVTVLQGCGPATPVPPTATPTLTPIPPPSPTATPVPGGARASTA
                     ARPNLSRNGQP"
BASE COUNT          268 a          649 c          579 g          285 t
ORIGIN      
        1 agttcgacga ctcaaatccc gcggcggcgc ccacggtggt cctcagttcc ctctttttcc
       61 ccacgggcct gcgcagggac ctggcgggca atttctacgt ggccgagtcg gacaacggcg
      121 gggtggccca attcctcgac ctctacgacc ccaccctgac gacccgggag aaccaatgcg
      181 ccctgccgga cgtctggggg gcggcggtca atgaaacggg ggaggtctac gtgagcgggc
      241 tcaacagctt cgccgtcacc gaggtccagg gctgcgcgct ggagcccacc gtgacgccca
      301 cccccgccta ccagggcgcc agtccgcccg gggcgggcgg ggcctttgtc tatccgtcgc
      361 ccgtgcgggg cgaccgggcc tcggtggcct accggttgcc ggcccccgcc ggggtgcggc
      421 tgaccgtttg gaacgaggcg ggggaaaaga tcaccgaggt gaccgaccac cggggcgccg
      481 gggtccaggc caccgctttc agcgtggcgg gctggtcccc cggggtctat ttctacgggc
      541 tcaccctttc ctatgattcg ggcggcgtcg agaagccgcc cttggggaaa ttcgcggtca
      601 tccattagaa gaccaaaagg ggaataaaag ggtccctgga cccactggga tcttgaccct
      661 cctgacgggg ggtccaagga gtccctcatg cgtatcccgg tccttttgcg gtccatcccc
      721 cggcggcggc cttcggcctg gcggccatgg cggcggccca aacgcccccc ctgggccccc
      781 tggcccaggt ccgatccatg ggcatcaccc tgccggttta tacctgcggg gccacgccgg
      841 tgccgggagg cacctgctgc cccgggctca cgtccctgga tgggttcgtg gcctccggcg
      901 gcatggccct ggacccggcc cgccatcggc tttacgccgc ggattttgag aacaaccgcg
      961 tccaggtctt cgacagccaa agcctcgccc gcgtgggcct gatctccgcc ctgggatcct
     1021 cgggggcgct caacctgccg gtggacgcgg ccctggacgc cgacggcaac ctctacgtgg
     1081 ccgacctggg caatgaggcg gtcgaaaagt tcgacgccaa cctccactac gtcgcctcca
     1141 tcgccgccgg gaagggcctg agcatcgtgg gggtctgggc cgagggggat tcggtctact
     1201 tctccaccct gcagaactat gtctaccgct acacccaagg cgtttccggt tactcggcct
     1261 ccgccacctt cggggagccg ggccaactca accatcccaa ccagatcgtg aagtcgggga
     1321 actggctcta tgtcaccgac acctacaacg accggttggt gaagttcgac gtcgaccacc
     1381 cggggcccgt gcccacgcag gtggtgggat gcctgctcat cccgaccggc ctgcggcccg
     1441 acgccacggg gaagtggttg ctggaggaat ccgacaatgg gggctatccg gcctatgtgg
     1501 acaccttcag ccacgacttc agcttgttga agagccgttg cgtcttcccc gacacctgga
     1561 gcgcggtgca ggaccccgcc acgggccgca ccttcgtgag cggcatgaac agcctgtcgg
     1621 tgaccgtgct gcagggttgc ggtccggcga cgccggtccc cccgaccgcc acccccaccc
     1681 tgacgcccat cccgccgccc agtcccacgg ccacgcccgt ccccgggggc gcccgggcct
     1741 cgacggcggc ccggcccaac ctgtcgcgca acgggcagcc c
//
LOCUS       DASTJJ010000191         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1741082, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000191 DASTJJ010000000
VERSION     DASTJJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741082"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1815
                     /locus_tag="VFR02_02350"
     CDS             <1..>1815
                     /locus_tag="VFR02_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HET9869327.1"
                     /translation="LPPEGEYGVGAIFLPKDHEQMRACEDMLAKIVEEEGQTLLGWRV
                     TPTDNTEIGLTARASEPIMKHVFIGKSKDLKDPDAFERRLFLIRKRLENQVAASSLPE
                     KGLFYVASMSCRTIVYKGMLTPGQVNSYFRDLRDKDTESALALVHSRFSTNTFPSWDL
                     AHPFRYIAHNGEINTLRGNSNWMHARQALFETEYFSPAEIQKLLPVIREGQSDSACFD
                     NALEILVMTGRSLPHAMMMMVPEAWTGHATMPQYKKDFYHYHSCLMEPWDGPASIAFT
                     DGRTIGALLDRNGLRPSRYYVTQDDLVVMASEVGVLDIDPKRVLKKGRLEPGKMFLID
                     MVQGRIVDDGELKEKIAKEHPYGEWLKENLVGLDKLPEAPHVPEPDHETVLKRQKAYG
                     YTHEDLRLLMAPMAINGEEAIGSMGDDTPIGILSNLNQPLFNYFKQLFAQVTNPPLDA
                     IREALVTSVNTTVGPEGNLLEPRPESCRQVELQSPVLDNDEMAKFTHISYKGFKSITL
                     PILYNVAEGGKGLAKAVEAVCAKASQAVKDGHQIIILSDRGLDHDLAPIPSLLAVAGV
                     HHHLIRQGERTKVGLLIESGEPREVHHFALLLGYGANA"
BASE COUNT          368 a          595 c          529 g          323 t
ORIGIN      
        1 cctgcccccc gagggggaat atggggtggg ggccatcttc ctgcccaagg accacgagca
       61 gatgcgggcc tgcgaggaca tgctggccaa gatcgtcgag gaggagggcc aaacccttct
      121 gggttggcgc gtcaccccga cggacaacac cgagatcggc ctgacggccc gggcttccga
      181 acccatcatg aagcacgtgt tcatcgggaa gtccaaggac ctgaaggatc cggacgcctt
      241 tgagcgcagg cttttcctca tccgcaagcg cctggaaaac caggtggcgg cctcgtcgct
      301 gcctgaaaag ggcctttttt atgtggcgag catgtcctgc cgtaccatcg tctataaggg
      361 gatgttgacc ccgggccagg tcaattccta tttccgcgac ctgcgggaca aggacaccga
      421 atcggccctg gcgctggtgc attcccgttt ctccaccaat accttcccgt cctgggactt
      481 ggcgcaccct ttccgctata tcgcccacaa cggggagatc aacaccctgc ggggcaacag
      541 caattggatg catgcccgtc aggcgctgtt cgagaccgaa tatttctcgc ccgcggagat
      601 ccagaaactc ttgcccgtca tccgggaagg ccagtcggat tcggcctgtt tcgacaacgc
      661 gctggagatc ctggtgatga ccgggcgttc cctgccccat gccatgatga tgatggtgcc
      721 cgaggcctgg acgggccacg ccaccatgcc ccaatataag aaggacttct accactacca
      781 ttcctgcctc atggagccct gggacggtcc cgcctccatc gcctttaccg acgggcgcac
      841 catcggcgcc ttgctggacc gcaacggcct gcgtccttcc cgctactacg tgacccagga
      901 cgacctggtg gtcatggctt ccgaggtcgg cgtgttggac atcgatccca agcgggtgct
      961 gaagaagggc cgcttggaac caggcaagat gttccttatc gacatggtcc agggccgcat
     1021 cgtggacgac ggggaactga aggaaaagat cgccaaggaa catccctatg gggaatggct
     1081 gaaggagaac ctggtcggct tggacaagtt gccggaagcc ccccatgtcc ccgagcccga
     1141 ccatgagacg gtcttgaagc gccaaaaggc ctatggctac acccacgagg acctgaggct
     1201 cctcatggcc cccatggcca tcaacgggga ggaggccatc gggtccatgg gcgacgatac
     1261 gcccatcggg atcctgtcga acctgaacca acccctcttc aactacttca agcagctctt
     1321 tgcccaggtc accaacccgc ctttggacgc catccgggaa gcgttggtca cttcggtcaa
     1381 caccaccgtg ggccccgagg ggaacctgct ggaaccccgc cccgaaagct gccgccaggt
     1441 cgaactgcaa agtccggtgt tggacaatga cgagatggcc aagttcaccc atatttccta
     1501 caagggcttc aagtccatca ccctccccat cctctataac gtcgccgagg gagggaaggg
     1561 gttggccaag gccgtggaag cggtctgcgc caaggccagc caagccgtca aggacggcca
     1621 ccagatcatc atcctgtcgg accgcggcct ggaccatgac ctggccccca tccccagcct
     1681 cttggcggtc gccggcgtgc accaccactt gatccgccag ggggaacgaa ccaaggtcgg
     1741 cctgctcatc gagagcggcg agccccgcga agtgcatcat ttcgccctgc tgttgggcta
     1801 tggcgccaac gccat
//
LOCUS       DASTJJ010000192         1297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_762563, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000192 DASTJJ010000000
VERSION     DASTJJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762563"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1297
                     /locus_tag="VFR02_02355"
     CDS             <1..>1297
                     /locus_tag="VFR02_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9869328.1"
                     /translation="RMKSAKLPAFSAEIYDGDTPTGTRAKIRKSPPHILLTNPDMLHL
                     GILAFHDAWAGFFKGLKYVVVDEAHSYRGVFGSHVAHVLRRLRRVCAHYGASPQFVTC
                     SATLGNPGEFLEGLTGLPFEVLSETGAPQNAKTFVLWNPDTASPYTEAVTLLIECLKA
                     GLKTIVFTKARKITELISLWVTQARPEWAAQIKSYRAGYLPEERREIEAELFQDELKA
                     VISTSALEVGIDVGGLDACVLVGYPGTMISTWQRSGRVGRGQAPSVVFLVAMADAMDQ
                     YWMKHPDRFFAMKPESLMVGFGNETIAGAHLRCAAAELPLTREADTAFYGALMDDLLP
                     GLVEKGSLLESAAGGKWLVTDKNPQRSVGIRDMGEGYQIIRQDTGELIGTIDGMRAFR
                     DCHPGAVYLHQGVQYVVKDLQWEGRKILVLDEPVDYYTQV"
BASE COUNT          226 a          438 c          421 g          212 t
ORIGIN      
        1 ggcggatgaa atccgccaaa ctgcccgcct tcagcgccga gatctacgac ggcgacaccc
       61 ccacgggcac ccgggccaag atccgcaaaa gcccgcccca tatcctgctc accaacccgg
      121 acatgttgca cctggggatc ctggccttcc atgacgcttg ggcgggtttt ttcaagggcc
      181 tgaaatacgt ggtggtggac gaggcgcaca gctaccgggg cgttttcggg tcgcatgtgg
      241 cccatgtgct gcggcgcctg aggcgcgtct gcgcccacta cggcgcctcg ccccaattcg
      301 tcacctgctc cgccaccctg ggcaaccccg gcgaattcct ggaaggcctc accggcctgc
      361 cctttgaggt gctgtccgag accggtgccc cccagaacgc caagaccttc gtgctttgga
      421 accccgatac ggcgtcgccc tataccgagg cggtgacgct gctcatcgaa tgcctgaagg
      481 cgggtttgaa gaccatcgtc ttcaccaagg cccgcaagat caccgagctc atcagcctct
      541 gggtcaccca ggcccgcccc gaatgggccg cccagatcaa gagctaccgg gcgggctacc
      601 tgcccgagga acgccgcgag atcgaggccg agctcttcca ggacgagctc aaggcggtca
      661 tctccacctc cgccctcgaa gtgggcatcg acgtgggcgg gttggacgcc tgcgtgctgg
      721 tgggatatcc gggcaccatg atctccacct ggcagcgctc gggccgggtg gggcggggcc
      781 aagcgccgtc cgtggtcttt ttggtggcga tggccgacgc catggaccag tactggatga
      841 aacacccgga ccgcttcttc gccatgaagc ccgaatccct catggtgggc ttcgggaacg
      901 agaccatcgc gggggcgcac ctgcgctgcg cggccgccga gctgcccctg acccgcgagg
      961 ccgataccgc cttctatggg gccttgatgg acgacctcct gcccggcctg gtggaaaagg
     1021 gaagcctctt ggaatccgcc gccgggggca agtggctggt gacggacaag aacccccagc
     1081 ggtccgtggg catccgggac atgggcgagg gttaccagat catccgccag gacaccgggg
     1141 agctgatcgg caccatcgac ggcatgcggg ccttccggga ctgccacccg ggggcggtct
     1201 acctgcatca gggcgtccaa tacgtggtga aggacctgca gtgggagggc cgcaagatcc
     1261 tggtcctgga cgagccggtg gattactaca cccaggt
//
LOCUS       DASTJJ010000193         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_639867, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000193 DASTJJ010000000
VERSION     DASTJJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639867"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFR02_02360"
     CDS             complement(<1..215)
                     /locus_tag="VFR02_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869329.1"
                     /translation="MTSPNAPPPAFDSFIRRWQGREGGQERANYGLFLSELCDAIGVP
                     RPDPASATTEENDYVFERAVKAAALEGP"
BASE COUNT          186 a          337 c          413 g          208 t
ORIGIN      
        1 gggccttcga gggcggcggc tttgacggcg cgttcgaaga cgtagtcgtt ttcttcggtg
       61 gtggcggagg caggatccgg gcgaggcacg ccgatcgcgt cgcacaactc gctcaggaac
      121 aggccgtaat tggcgcgctc ctgcccgcct tcgcggcctt gccagcgccg gatgaaggag
      181 tcgaacgccg ggggtggtgc attgggcgag gtcacggatt gggtcacgtg ggggcggggg
      241 ctgcatgcga ttgcgtgatg cgctgtacag cccggttcgg ttcgggggca ataaggtttg
      301 tgtggcgcgg cggcgccggc ctacatgcga acgcccgtca ggatcggcct gacgggcgtt
      361 caaggaggag aagatggctc gtatccatcg ttgtctcctg ccgtggatcg tggtgatcct
      421 gaccgtaaag gtcaagatca gcatagagaa gcggtagtgg gcgggccagt ccgggaaacc
      481 gggctggcct tcctcccgtg aacggctccg cgcccggcgg ggcgaatcgg aggtgggata
      541 atcgtgttgg gggccctggt ttccggctct ccgcttcgct ccggcgggga tgacggacag
      601 cgacgtgggc tctggccggg cgacgaggat ggtctcggtg gaatcggcgg agaggactct
      661 ggaatccccc acctcacctg gcctctccgc ccccgagggc tacggtattc acacaagtgg
      721 cggaggagcc cggagcggag aaaaattctc cccctcccgc ttgcgggagg gggtaggggg
      781 agggctcgtt cggacagtga ccaaccccct caccctgccc ctctcccccg caaggcgggg
      841 gagagggaaa attcttgcta ccccgacagg acttgtgaat ccgatagccc cgaggggcgg
      901 agaggaaatg ttttggctgg gtcggtgtga acgcatcatg ttctaatccc ctggacactg
      961 cgaagcccgg gccgcatagg ggcgcacacg aatcagtgtc aaactagcgc ccctggggag
     1021 ccgcctgccc gccgatgatg tatttcagcc gcctgaagac gctctcgatc ctgggcgcct
     1081 gccttgtggg attgctgctg tgcatcccca acctgatacc cgccccggcg ccgtggctgc
     1141 cgtg
//
LOCUS       DASTJJ010000194         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1619385, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000194 DASTJJ010000000
VERSION     DASTJJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619385"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFR02_02365"
     CDS             <1..555
                     /locus_tag="VFR02_02365"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET9869330.1"
                     /translation="LALGTALAFHFHGVRAPRPAGIEALGGSQPLYDPADKPIKVNLK
                     GVNPATGKWSDFPVTIRQSKSRLNQMKQAVLAFLEDAPRLDIPAGLSLNELYWSQHTG
                     AVVDLSTADLPEGSMGFYEELLVVRGLIETLSKNFPEVRAVKILVNGQEAPTVAGHYA
                     LGTTEAAAPVAPAANPFEETYGNQ"
     gene            566..>1408
                     /locus_tag="VFR02_02370"
     CDS             566..>1408
                     /locus_tag="VFR02_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9869331.1"
                     /translation="MPSAYDPKDLEGRWYESWEKAGYFTASTDGGKPAFSMVIPPPNV
                     TGSLHMGHALNHTLQDLIARYKRMRGFNVLWLPGTDHAGIATQNVVERELRKEGKTRQ
                     DLGREAFVKRVWEWKEKYGATITRQIRRMGASCDWTRERFTLDEGLSRAVRKVFIDLY
                     NEKLIYQDYYLINWCPRCQTALSDIEVEHKPTQGSFYHIRYPFVHDPADGLEVATTRP
                     ETLLGDTAVAVHPDDDRYAGLDGKSVTVPLAGRRIPILHDAYVDKDFGTGVVKITPAH
                     DFNDF"
BASE COUNT          258 a          488 c          450 g          212 t
ORIGIN      
        1 ctggccctgg ggacggcctt ggcgttccat ttccacggcg tgcgggcccc ccgcccggcg
       61 gggatcgagg cgctgggggg cagccaaccc ctctatgacc ccgcggataa gcccatcaag
      121 gtcaacctga agggcgtgaa ccccgccacg ggcaaatggt ccgattttcc ggtcacgatc
      181 cgccagagca agagccggct gaaccagatg aaacaggccg tgctggcctt cctggaggac
      241 gcgccgcgcc tggacatccc ggcggggctt tccctgaacg aactttattg gtcccagcac
      301 acgggggccg tggtggacct ttccaccgcc gacctgccgg aaggctccat ggggttttac
      361 gaggaactgc tcgtggtcag ggggctcatc gagaccctgt ccaagaattt ccccgaggtg
      421 cgggcggtca agatcctggt gaacggccag gaggccccca cggtggcggg ccattacgcc
      481 ctggggacca ccgaagccgc ggcgcccgtg gccccggcgg ccaacccctt tgaggaaacc
      541 tatggaaacc aatgacccga ccctgatgcc ttccgcctac gaccccaagg acctggaggg
      601 gcgttggtac gagtcctggg agaaggccgg ttatttcacg gcctccacgg acggcggcaa
      661 gcccgccttt tccatggtca tcccgccccc caacgtgacg ggcagcctgc acatgggcca
      721 cgcgctcaac cacaccctgc aggacctcat cgcccgctac aagcgcatgc gggggttcaa
      781 cgtgctgtgg ctgccgggca cggaccacgc gggcatcgcc acccagaacg tggtggagcg
      841 ggaactgcgc aaggaaggca agacgcgcca ggacctgggc cgggaggcct tcgtcaagcg
      901 cgtgtgggaa tggaaggaaa agtacggggc caccatcacc cggcagatcc gccggatggg
      961 cgcctcctgc gactggaccc gggagcggtt caccctggac gaggggcttt cgcgcgccgt
     1021 gcgcaaggtc ttcatcgacc tctacaacga gaagctcatc taccaggact actacctcat
     1081 caactggtgc ccgcgctgcc agaccgcgct gtccgacatc gaggtggagc acaagcccac
     1141 ccagggttcc ttctaccaca tccgctatcc cttcgtgcac gaccccgccg acgggttgga
     1201 agtggccacc acccggcccg agacgctgct gggcgacacc gcggtggccg tgcatcccga
     1261 cgacgaccgc tacgcggggc tggacggcaa gtccgtgacc gtgccgctgg ccggccggcg
     1321 catccccatc ttgcatgacg cctacgtgga caaggatttc gggaccggcg tggtcaagat
     1381 caccccggcg cacgacttca acgacttc
//
LOCUS       DASTJJ010000195         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_640230, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000195 DASTJJ010000000
VERSION     DASTJJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640230"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..757
                     /gene="tmk"
                     /locus_tag="VFR02_02375"
     CDS             107..757
                     /gene="tmk"
                     /locus_tag="VFR02_02375"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9869332.1"
                     /translation="MKKGLFITFEGTEGSGKSTQLRRLGALLAKHRIPHLLTREPGGS
                     RLSTHLRRWILNRLDYRLAPKTELHLFLADRAQHVQEVLGPALRAGKVVLCDRYTDST
                     LAYQGGGRGFDLKLLEMMNRDATGGLTPDWTLLFDLPVEVGLRRALGRGRGRDRMEAE
                     KLRFHRAVRRVFLRTARKDKKRFIVLDAARSPAEVYQQMLEGLIDRLPGPRVGRRG"
     gene            750..>1299
                     /locus_tag="VFR02_02380"
     CDS             750..>1299
                     /locus_tag="VFR02_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9869333.1"
                     /translation="MADFTVPRLEGLDSQPEARALLERILAGGQAPTQLWTGPEGCGK
                     KTHALALTRTLFCLEGPGCKGCAACRQVLNRTHSDFFWLAREEGKNEVTVESVRELDK
                     KLSSAPLSAPWKVAVLPEADRMNRDAQNAFLKTLEEPPAKTLVILLAERSASFLPTVL
                     SRCRPVRFGALPEAKLEGILSKA"
BASE COUNT          214 a          415 c          453 g          217 t
ORIGIN      
        1 ttggccaccc tgatccatca gggcggcggc aagcggccca acgcgggcta tatcgcctat
       61 gtgcggggtt tgagcgggtc aaaataagaa ggatgaaggg gcggatttga aaaagggatt
      121 gttcatcacc ttcgagggca ccgagggctc gggaaagagc acccagttaa ggcgcctggg
      181 ggccttgctg gcaaaacacc gcatccccca tctgctgacc cgggaaccgg gcggatcgcg
      241 tctgagcacc cacctgcgcc gctggatcct caaccggttg gattaccgct tggcgcccaa
      301 gaccgagctc caccttttcc tggccgaccg ggcccaacac gtgcaggagg tcctgggacc
      361 ggccctgcgg gcgggcaagg tggtgctttg cgaccgctac accgattcca ccctggccta
      421 ccagggcggg ggccggggct tcgacctgaa gttgctggag atgatgaacc gggacgccac
      481 cgggggattg acccccgact ggacgttgtt gttcgacctt cccgtggagg tggggctacg
      541 gcgggccttg ggccgcggcc gcggtcggga ccgcatggaa gccgagaagc tccggttcca
      601 tcgggctgtc cgccgggtgt tcctccggac ggcccggaag gacaagaagc ggttcattgt
      661 cctggacgcc gccaggtccc cggcggaagt ctaccagcag atgctggagg ggctcatcga
      721 ccgcctgccg ggcccccggg tgggccgccg tggctgattt caccgtcccc cgattggaag
      781 ggctggattc ccaacccgag gcccgggcct tgctggaaag gatcctggcg ggcggccagg
      841 cgccgaccca gctctggacg ggcccggaag ggtgcggcaa gaagacccac gccttggcct
      901 tgacccggac gctcttttgc ctggagggcc ccggctgcaa gggctgcgcg gcctgccggc
      961 aggtgttgaa ccggacccat tcggactttt tctggttggc ccgggaagag gggaagaacg
     1021 aggtcacggt ggaatccgtc cgggagttgg ataaaaaact ttccagcgcc ccgctgtcgg
     1081 cgccctggaa ggtcgccgtt ctgcccgagg cggaccgcat gaaccgggac gcccagaacg
     1141 ccttcttgaa gaccctggag gagcctccgg ccaagaccct ggtgatcctg ctggccgaga
     1201 ggtccgcgtc cttcctgccc acggtccttt cccggtgccg tccggtgcgc ttcggcgcct
     1261 tgccggaggc caagctggag ggcatcctgt cgaaggcct
//
LOCUS       DASTJJ010000196         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1619509, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000196 DASTJJ010000000
VERSION     DASTJJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619509"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFR02_02385"
     CDS             complement(<1..817)
                     /locus_tag="VFR02_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869334.1"
                     /translation="MEPRMVPPPPKRSFVKRFWPVLPLALLGLGLGGYADHRAKHPKI
                     KVILPAPLPPAFETLPVGSVKPLGWLKQQMQLQASGLTGHLDEFWPDISQSGWIGGKG
                     EGWERAPYWLDGLVPLAFELDDPALKQKARRWVDYILAHQSPDGWLGPDQGASNYGFQ
                     LPPNPVRDPWPQFVILKALAQYAEATGDPRVMPAMARDLANLDQQLDRRPLFDWSFFR
                     WGDLLVTLDWMAARTGDPRWPRMEAKAAAQGYGWAGHFADFHERQKTVEWNFLS"
BASE COUNT          231 a          347 c          352 g          199 t
ORIGIN      
        1 ggctcaggaa gttccattcg acggtctttt gcctctcatg gaaatcggcg aaatggccgg
       61 cccagccgta gccctgggcg gcggccttgg cctccatccg cggccaccgg gggtcccccg
      121 tgcgggcggc catccagtcg agcgtcacca gcaggtcgcc ccaccggaaa aaggaccaat
      181 cgaaaagggg ccgccggtcg agctgctggt cgaggttggc caggtcccgc gccatggcgg
      241 gcatcacccg cgggtcgccg gtggcttccg cgtattgggc gagcgccttg aggatgacga
      301 actggggcca ggggtcccgg accgggttgg ggggcagttg gaagccgtag ttggacgcgc
      361 cctggtcggg gcccagccac ccgtcgggcg actggtgggc caggatgtag tccacccacc
      421 ggcgggcctt ttgcttgagc gccgggtcat ccagctcaaa ggccaggggc accaggccgt
      481 ccagccagta gggcgccctt tcccagccct cacccttgcc gccgatccat ccgctttggg
      541 agatatcggg ccaaaattca tccaggtggc cggtaaggcc ggaagcctgc agttgcatct
      601 gttgcttgag ccaccccaag ggtttgacgc tgcccaccgg cagggtctcg aaggcgggcg
      661 gcagcggggc cggaaggatg accttgatct tggggtgctt ggcgcggtgg tcggcgtagc
      721 ccccaaggcc caggccgagg agcgcgaggg gaagaacggg ccaaaaccgc ttgacgaagg
      781 aacgcttggg gggcggcggg accatccggg gttccattga ggccgctcct atttttcctg
      841 gttcctgtcc cggcgccctc ggcggcggtc cttcaagata ggatgcaaca aaacccaccg
      901 gcaagaaagg gtttagctga taataatcac atcgaaacac aacccatccc caaccttcaa
      961 ccccaacgga gatattcatg acgtcaataa caaccatcat cattccagcc atttgtttga
     1021 tcgcgggcca actattccaa tattttctaa caagacgtca acagcacgaa caagaaatac
     1081 agaaattgaa gatcgaagct tattcaaact tcattaaagc cacgacttc
//
LOCUS       DASTJJ010000197         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1251716, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000197 DASTJJ010000000
VERSION     DASTJJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251716"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1110
                     /locus_tag="VFR02_02390"
     CDS             91..1110
                     /locus_tag="VFR02_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET9869335.1"
                     /translation="MKITVYFGPKSVKPTVVQNATVVLADVLRASSTANTYLYHGAKG
                     VFFAPDPGTAKKYFSKLKRGEGLLAGERDWEKIPGFHLGGSPLDSSRDKVKDRIVCLS
                     SPSFVAHKPAKGGKYVILGSFVNLGDVYDSCLRGGRDVVIVTSGAPEDGVFAGMLVDF
                     LQSTVGAVPILPAESAKEAMALYKPWQGRLLDLLKESPEGKELIRKGFAKDLDFTARP
                     SRISAVGFLSGDLFFRENSPKPKKSLEGDSRSKSGPVAQKGKSIKVQVPLFPKNPEHP
                     VPGLDRKKKDAPPAAPLDPKKKPEGKKPEGAPKSKRAPRKPTAMFSKKTVATAGAAPV
                     VKKKI"
     gene            1107..1550
                     /locus_tag="VFR02_02395"
     CDS             1107..1550
                     /locus_tag="VFR02_02395"
                     /inference="COORDINATES: protein motif:HMM:NF036812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869336.1"
                     /translation="MTASRGAGGPAGRPSPRALLRVLDANCNRVREGLRVAEDAARFL
                     LNDPALTARLKRLRHAVTAAEKALFRSGLSRTGARDVAGDRGRGDRERSEKRRDGVKD
                     LIRANLKRAQEGLRSLEEFSKLKGGPVGARFKKIRYECYRVEEGL"
     gene            1635..>2895
                     /gene="thiC"
                     /locus_tag="VFR02_02400"
     CDS             1635..>2895
                     /gene="thiC"
                     /locus_tag="VFR02_02400"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET9869337.1"
                     /translation="MSNQKNSQPSESILTRKPFPGSSKVYVGGKTHHLHVPMRQIQLS
                     PTKTEKGLEENAPLLVYDTSGPYTDPTVSIDLRKGLSPLRRDWVMGRGDVEEVEGFYQ
                     GKSAPGVEVFPEASRRKPLKAKTGGNVSQMHYAKKGIITPEMEFIAIRENQLLQERAE
                     KGRFHPGQAFGAVMPRTITPEFVRDEVARGRAIIPANINHLEVEPMVIGRNFLVKINT
                     NIGNSAVASSIEEEVEKMVWSVRWGGDTLMDLSTGKNIHETREWILRNCPVPVGTVPI
                     YQALEKVGGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAWRRTGIVSR
                     GGSIHAKWCLAHHQENFTYTHFEEICEIMKAYNVSFSLGDGLRPGSIEDANDEAQFSE
                     LKTLGELTQKAWQHDVQVMIEGPGHVPM"
BASE COUNT          575 a          959 c          930 g          431 t
ORIGIN      
        1 ggcaaggaag aggccaagcc caaggccaag gactgacgcg ggcttgaaac ccgggccggg
       61 gttgcgcatc ttgcgggtag ggagagaaaa ttgaaaatca cggtttattt cggtccgaaa
      121 tcggtcaagc cgacggtggt ccagaacgcc acggtggtgc tggccgacgt gctgcgggcg
      181 agttccacgg ccaacaccta cctgtaccac ggcgccaagg gggtcttctt cgcccccgac
      241 cccgggacgg ccaagaaata tttctccaag ctcaagcgcg gcgagggcct gctggccggg
      301 gaacgggatt gggaaaagat cccgggtttc cacctgggcg gctcccccct ggattcgagc
      361 cgggacaagg tcaaggaccg gatcgtgtgc ctcagcagcc cctcctttgt ggcccacaag
      421 ccggccaagg gcggcaagta cgtcatcctg ggttccttcg tgaacctcgg cgatgtgtat
      481 gattcctgcc tgcggggcgg gcgggacgtg gtcatcgtca ccagcggggc gcccgaggac
      541 ggggtcttcg ccggcatgct ggtggacttc ctgcaaagca cggtgggcgc ggtccccatc
      601 ctgcccgcgg aaagcgccaa ggaagccatg gccctctaca agccctggca gggccgcttg
      661 ttggacctgc tcaaggaatc ccccgagggg aaagaactca tccgcaaagg tttcgccaag
      721 gacctggatt tcaccgcccg ccccagccgc atctcggcgg tcgggttcct gtcgggcgac
      781 ctgttcttcc gcgagaactc gcccaagccc aagaagtcgc tggagggtga ttcgcggtcc
      841 aagtccggtc cggtggccca gaagggcaag tccatcaagg tgcaggtgcc cctcttcccc
      901 aagaaccccg aacatccggt ccccggcctg gaccgtaaaa agaaggatgc gccccccgcc
      961 gcgcccctgg accccaagaa gaaacccgag ggcaagaagc ccgagggggc gcccaagtcc
     1021 aaacgggccc cgcgcaagcc cacggccatg ttcagcaaaa agaccgtcgc caccgccggg
     1081 gccgccccgg tggtgaaaaa gaagatttga ccgcctcccg gggcgcgggc ggcccggcgg
     1141 gtagaccgtc gccccgcgcc ctcctccggg tgctggacgc caattgcaac cgggtgcggg
     1201 aaggcctgcg ggtggccgag gacgccgccc ggttcctcct caacgacccc gccttgacgg
     1261 cccggctcaa gcggctgcgc cacgccgtga cggccgccga gaaggccctc ttccgatccg
     1321 gcctttcccg tacgggcgcg cgcgacgtgg cgggcgaccg gggccggggc gaccgggaaa
     1381 ggtccgaaaa gcggcgtgac ggcgtcaagg acctgatccg ggccaacctc aaacgggccc
     1441 aagagggctt acggtccctc gaggaattct ccaagctgaa gggcggaccg gtgggcgccc
     1501 gcttcaagaa gatccgctac gaatgctacc gggtggaaga gggtctctga cccgggttcc
     1561 cgttgcattc gctttaggcc cgcactatgc tgggtggatt caccgccgtc atcctgaaat
     1621 cccgggggaa ccgcatgtcg aaccagaaaa acagccagcc cagcgagtcc atcctgaccc
     1681 gcaaaccctt cccgggatcg agcaaggtct atgtgggcgg caagacccac cacctccatg
     1741 tccccatgcg ccagatccag ctttccccca ccaagaccga gaaggggctg gaagagaacg
     1801 cccccctctt ggtctatgac accagcggcc cctacacgga cccgaccgtg tcgatcgacc
     1861 tcaggaaagg gttgagcccc ctgcgccggg attgggtgat gggccggggc gacgtggagg
     1921 aagtggaggg cttttaccaa ggcaagagcg ccccgggcgt ggaggtcttc cccgaagcct
     1981 cgcgccgcaa acccctcaag gccaagacgg ggggcaatgt cagccagatg cactacgcga
     2041 agaagggcat catcaccccg gagatggaat tcatcgccat ccgggagaac caattgctgc
     2101 aggagcgggc cgagaagggc cgcttccacc cgggccaggc ctttggagcc gtcatgccca
     2161 ggaccatcac ccctgaattc gtgcgcgacg aggtggcccg gggccgcgcc atcatcccgg
     2221 ccaacatcaa ccacctggaa gtcgagccca tggtcatcgg gcgcaatttc ctggtgaaga
     2281 tcaacaccaa catcggcaac tccgccgtgg cctcctccat cgaggaagaa gtggaaaaga
     2341 tggtctggtc cgtgcgctgg ggcggggaca ccttgatgga cctctccacg gggaaaaata
     2401 tccatgagac ccgcgagtgg atcctccgca actgccccgt gccggtgggg accgtgccca
     2461 tctaccaggc cctggaaaag gtcggcggcg tggccgagga cctgacctgg gagatctacc
     2521 gggataccct catcgaacag gccgagcagg gggtggacta tttcaccatc cacgcggggg
     2581 tgctcttgcg ctacgtgccc ctgacggcct ggcgccggac cggcatcgtg tcccggggcg
     2641 gctccatcca cgccaagtgg tgcctggccc accaccagga gaacttcacc tacacccatt
     2701 tcgaggagat ctgcgagatc atgaaggcct acaacgtttc cttcagcctg ggggacggcc
     2761 tgaggcccgg ttccatcgag gacgccaacg acgaggccca gttctccgaa ctgaagacct
     2821 tgggggaact cacccaaaag gcctggcaac acgacgtgca ggtcatgatc gagggtccgg
     2881 gccacgtgcc catgc
//
LOCUS       DASTJJ010000198         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_274127, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000198 DASTJJ010000000
VERSION     DASTJJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274127"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFR02_02405"
     CDS             complement(<1..293)
                     /locus_tag="VFR02_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HET9869338.1"
                     /translation="MAAEELITLFRDCEGTAIPAGTKTQLYKDEKVYLTQTLGGSFTV
                     QRDNGQLVRVEGKNADALGKPVPESGGVVKMNFDPTQPIDDAQLEKEVWNTLRT"
     gene            complement(293..757)
                     /locus_tag="VFR02_02410"
     CDS             complement(293..757)
                     /locus_tag="VFR02_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9869339.1"
                     /translation="MSDLSDLYQELILDHQKNPRNFGQIEGANHHAEGFNALCGDRIS
                     LDLLTDGEGVIQDLKFRGSGCAISKASASMMTAALKGKKVGEAEAVFHQFHDLVTGKS
                     APETGEALGKLKVFAGVRDYPARVKCASLAWHTLEAALHDEQGVVSSDEEEA"
     gene            complement(757..>1592)
                     /locus_tag="VFR02_02415"
     CDS             complement(757..>1592)
                     /locus_tag="VFR02_02415"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET9869340.1"
                     /translation="WQMLAEEKGAKIRVVPIDDAGEFLLDEYRKLLTPKTKLVAVTQV
                     SNALGTVTPLRDIIRLAHDQGAKVLVDGAQAVAHQAVDVRGMDCDFYVFSAHKLYGPT
                     GVGVLYGKLALLNAMPPYQGGGDMIATVTFEKTTYKKAPYKFEAGTPNIAGGVALTAA
                     LDYVTALGFEAIEAQERDLLAYGTERLSRVPGLRIIGTAREKAGVISFTLGEVHPHDI
                     GTILDRQGVAIRAGHHCAQPVMDRFGVPATARASFALYNTREEVDALEAAVRKVMEVF
                     G"
BASE COUNT          228 a          527 c          525 g          312 t
ORIGIN      
        1 gtccgcaggg tgttccagac ctccttttcc agctgggcat cgtcgatggg ctgggtgggg
       61 tcgaagttca tcttcaccac gcccccggac tcggggacgg gcttgcccag ggcgtcggcg
      121 ttcttgcctt ccacccgcac cagctgcccg ttgtcccgtt ggaccgtgaa actgccgccc
      181 agggtctggg tcaggtacac cttctcgtcc ttataaagct gggtcttggt gccggccggg
      241 atggccgtgc cctcgcagtc ccggaatagg gtgatgagtt cctcggccgc catcaggctt
      301 cctcctcgtc gctggaaacg accccttgtt cgtcatgcag ggcggcctcc agcgtatgcc
      361 aggccaggct ggcgcatttg acgcgcgcgg gatagtcccg gacgccggcg aagaccttca
      421 gttttcccag ggcctcgccg gtctcggggg cgctcttgcc cgtcaccagg tcgtggaatt
      481 gatggaagac ggcctccgcc tcgcccactt tcttgccctt gagggccgcc gtcatcatgg
      541 aagccgaggc cttggagatg gcgcagccgc tcccccggaa cttcaggtcc tggatgaccc
      601 cttccccgtc ggtcaacagg tccagcgaga tgcggtcgcc gcagagcgcg ttgaaccctt
      661 cggcgtggtg gttggcaccc tcgatctgcc cgaagttcct cgggttcttc tggtggtcca
      721 ggatgagttc ctggtatagg tcgctcaggt cggacatcag ccgaacacct ccatgacttt
      781 ccggacggcc gcttccaggg cgtccacctc ttcccgggtg ttgtagagcg cgaaggaggc
      841 ccgggccgtg gccggcaccc cgaagcggtc catgaccggt tgggcgcaat ggtgccccgc
      901 gcggatggcc acgccctggc ggtccaggat ggtcccgatg tcgtggggat ggacctcccc
      961 cagggtgaag gagatcaccc cggccttttc ccgggccgtt ccgatgatcc ggagtccggg
     1021 gacccgggaa agcctctcgg tgccgtaggc cagcaggtcg cgctcctgcg cctcgatggc
     1081 ctcgaacccc agggccgtga cgtaatccag ggccgcggtc aaggccaccc ctcccgcgat
     1141 attgggggtc ccggcctcga atttgtaggg ggccttttta taggtggtct tttcgaaggt
     1201 caccgtggcg atcatgtcgc ccccgccctg gtagggcggc atggcgttca acagggccaa
     1261 tttgccgtag agcacgccca cgcccgtggg gccgtaaagt ttgtgggcgg agaaaacgta
     1321 gaaatcgcag tccatccccc ggacgtccac ggcctggtgg gccaccgctt gggcgccgtc
     1381 caccagcacc ttggccccct ggtcatgggc caggcggatg atgtcccgca ggggcgtgac
     1441 cgtgcccagg gcgttggaga cctgggtgac cgccaccagc ttggtcttgg gggtcaggag
     1501 cttgcggtac tcgtccaaca ggaactcgcc ggcgtcgtcg atgggcacca cgcggatctt
     1561 ggcgcccttt tcctcggcca gcatctgcca gg
//
LOCUS       DASTJJ010000199         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1252546, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000199 DASTJJ010000000
VERSION     DASTJJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252546"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFR02_02420"
     CDS             complement(<1..636)
                     /locus_tag="VFR02_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869341.1"
                     /translation="MNRPRSGPWGAFLPLVPVLGLVWERFHTYWEAQQPAVTTAALIG
                     HGLLQGRPLYSDLWDPHPPALSLSYALAEALGGDGPYSIFLLGLGTGLGTLAALTLAG
                     RAYGGPAAGLLAACLWAIVSGDPTVWANQPLAGSFVNLFAAWALWFLLEEGKGRPGTR
                     AKLAAGTLLGIASLYQLSVVALGAAWALAGAFLDRSEGRRPDPGWAWVALGA"
     gene            complement(633..1616)
                     /locus_tag="VFR02_02425"
     CDS             complement(633..1616)
                     /locus_tag="VFR02_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9869342.1"
                     /translation="MDPSPIPLTVADPFGGQRLDVFLASQLPDLSRARVRKLIDGGRV
                     EAAFAVKAVKANLLVSVGQTFRVTLPPPEPTHLPAQPLALDLLFEDQDLLVINKPAGL
                     VVHPGAGNPDHTLINALVAHCPRIQGVGGVNRPGLVHRLDKDTSGLLVVAKSDLAFKG
                     LVRELKGRHLRRIYLGLVKGRLEGKGKVDAPLGRHPGARNKMSVRPDGGKEAVTHFES
                     IRSGEEASLLMFKLETGRTHQIRVHAQFIKHPIWGDPVYGGPTAGAGRQMLHAFRLTF
                     RHPRTKETLSFLAPPPADLTACARSLGLSPLSWESLDWKDPEAPARPRKKA"
     gene            complement(1663..2484)
                     /locus_tag="VFR02_02430"
     CDS             complement(1663..2484)
                     /locus_tag="VFR02_02430"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET9869343.1"
                     /translation="MHPILFSFGTLRLFGWEIGPIQLYSYGLCLALALGLSITLFARD
                     AGRYIAPRVGLSRKEGFQRTFDLAVWVILCSIAGARIFYALENHTEFTGRWVAVLFVW
                     QGGLVYYGGLFGGALAGVLWFLKHRWPLAYSADVLAPYISLGQAIGRMGCFLNGCCYG
                     IVDEKHGLVFPGIGDGLPHLPTQLWELYGDLALFAGLWWARRFTLAYPWMTLSAYGLT
                     YGMLRFGIEFWRRDWDKRYLGYFVSASQAVSALLIAVSLAVLVAILWRGPKKAAR"
     gene            complement(2513..>2971)
                     /gene="lspA"
                     /locus_tag="VFR02_02435"
     CDS             complement(2513..>2971)
                     /gene="lspA"
                     /locus_tag="VFR02_02435"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9869344.1"
                     /translation="GWGALAAYGLDQASKLLVLHSMSLGRSWPLWGDAVRLTYIQNPG
                     SAFGLLASAPAIWRVPFFCLVTLGASVMIYAFQRFLPPGKAQVRRLALGLVWGGALGN
                     FTDRIWLGKVIDFIDVGYRQYRWPVFNLADSCITVGLACLVLEMIFRDRH"
BASE COUNT          609 a          989 c          982 g          391 t
ORIGIN      
        1 cgcgcccagg gccacccacg cccatcccgg atcggggcgg cgtccctcgg accggtccag
       61 gaaagccccc gccagggccc aggcggcgcc caaagccacc acggacaact gataaaggga
      121 cgcgatcccc agcaaggtcc cggcggccag cttggcccgg gtccccgggc gcccttttcc
      181 ctcctccaac aggaaccaaa gggcccaggc cgcgaacagg ttcacgaagc tcccggccaa
      241 gggctggttg gcccagaccg tcggatcccc tgagacgatg gcccaaagac aggcggccaa
      301 gagtcccgcc gcgggcccgc cgtaagcccg gcccgccagg gtcagcgccg ccagggtccc
      361 cagtcccgtt cccagcccga gcaggaagat cgaatagggg ccgtcaccgc ccaacgcctc
      421 ggccaaggcg taggacaggg acaaagcggg cggatgggga tcccacaggt ccgaatagag
      481 cggccgtccc tgcagaagtc catgtccgat gagcgcggcg gtggtgacgg cgggctgttg
      541 ggcttcccaa taagtatgga acctttccca caccaggccc aggacgggca ccagcggaag
      601 gaaggccccc caagggccgg accggggacg gttcacgctt tcttgcgggg ccgggccggc
      661 gcctccgggt ccttccaatc gagcgattcc caggaaagcg gcgagagccc cagggaccgg
      721 gcgcaggccg tgagatcggc cggcggaggg gccaagaacg agagggtctc cttggtccgg
      781 ggatggcgga aagtcagccg gaaggcgtgc agcatctggc gaccggcgcc cgccgtgggg
      841 ccgccgtaga ccgggtcccc ccagatggga tgcttgatga attgggcgtg cacccggatc
      901 tggtgggtgc gtccggtctc caacttgaac atcaaaaggc tggcttcttc cccggaccgg
      961 atggactcaa aatgggtgac ggcttccttg ccgccgtcgg gccgcacgct cattttgttg
     1021 cgcgctcccg gatggcgacc caggggggcg tccaccttgc ccttcccctc cagccttccc
     1081 ttgaccaggc ccaggtagat gcgcctcaaa tgccggccct tcagttcgcg gaccaggccc
     1141 ttgaaggcca ggtccgattt ggccacgacc agcagtcccg aggtgtcctt gtccaaccgg
     1201 tgcaccagtc ccggccggtt cacgcccccc acgccctgga tccgggggca atgggccacc
     1261 aacgcgttga tgagggtgtg gtccgggttc cccgcccccg gatgcaccac caacccggcg
     1321 ggtttattga tcaccaacag gtcctggtcc tcgaagagca ggtccaacgc caagggttgg
     1381 gcgggcaggt gggtgggctc ggggggcggc agggtgaccc ggaaggtctg acccacgctc
     1441 accaacaggt tggccttgac ggccttgacc gcgaaggcgg cctccacccg gcccccgtcg
     1501 atgagcttgc gcacccgggc ccgcgagaga tcgggcaatt gggaggccag gaagacgtcc
     1561 aaccgctggc cgccgaaggg gtcggccacg gtcaagggga tcggggaagg gtccatggtg
     1621 gggattatag gggaatccgg ggggaaaata ccggcgcgcc cctcagcggg ccgccttttt
     1681 cggcccccgc caaaggatgg ccaccaagac cgccagggaa accgcgatca acaaggcgct
     1741 caccgcttgg gaggcggata cgaaatagcc caggtagcgt ttgtcccaat cccgccgcca
     1801 gaattcgatc ccgaaccgca gcatgccgta ggtgagccca taggcgctca acgtcatcca
     1861 aggataggcc agggtgaatc ggcgggccca ccagagcccc gcgaagaggg ccaggtcgcc
     1921 gtaaagttcc cataattggg tgggaagatg gggcaggccg tccccgatgc ccgggaagac
     1981 caacccgtgc ttctcatcca cgatgccgta acaacagccg ttcaggaaac atcccatgcg
     2041 cccgatggcc tggcccaggg aaatgtaggg ggccagcacg tcggccgaat aggccagggg
     2101 ccagcggtgt ttgaggaacc agagcacccc ggccagggcc ccgccgaaga ggccgccgta
     2161 atagaccagc ccgccctgcc agacgaaaag caccgcgacc cagcggcccg tgaactccgt
     2221 gtggttctcc agggcgtaaa agatccgggc gccggcgatg gaacaaagga tgacccaaac
     2281 cgccaggtcg aaggtgcgtt ggaatccctc cttacgggac aacccgaccc ggggcgcgat
     2341 gtagcggccc gcgtcccggg cgaagagggt gatgctcaac cccagcgcca aagccaggca
     2401 aagcccgtag gaataaagct ggatgggacc gatctcccag ccaaaaaggc gcaaggtgcc
     2461 gaaggaaaaa aggatgggat gcacaaaacc tcctgagaag gggaaagccg ggtcagtgcc
     2521 ggtcccggaa gatcatttcc agcaccaggc aggccagtcc cacggtgatg caggaatccg
     2581 ccaggttgaa gaccggccat cggtattgtc ggtagcccac atcgataaaa tcaatgacct
     2641 tgcccagcca aatgcggtcg gtgaagttgc ccaaggcccc gccccacacc agccccaggg
     2701 ccaaacgccg tacttgggcc ttccccggcg gcaggaagcg ttggaacgcg tagatcatca
     2761 ccgacgcccc cagtgtcact aggcaaaaaa aagggacgcg ccaaatggcc ggcgccgagg
     2821 ccagcaggcc gaaggccgat ccggggttct ggatataggt gagcctcacc gcgtcgcccc
     2881 agaggggcca ggatcggccc aaggacatgg aatgcaggac cagcagtttg gacgcctggt
     2941 ccaagccgta ggccgccaag gccccccacc c
//
LOCUS       DASTJJ010000200         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1252737, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000200 DASTJJ010000000
VERSION     DASTJJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252737"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFR02_02440"
     CDS             complement(<1..1065)
                     /locus_tag="VFR02_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET9869345.1"
                     /translation="MDLTFMVVLVIALALFFDFTNGFHDTANAMATPIATGAIKPKTA
                     VALAAILNLVGAFLSTEVAKTISGGIINEGPGGVAITPEMIFAGLMGAVLWNLFTWLL
                     GLPSSSSHALFGGLIGAAIVGSWSFGAVNFLVVLSKVIIPALLAPAVAGGVAYLCTRM
                     AYAITRRSDPDSGDKLTQKRGGFRHGQVFSSSLVALAHGTNDAQKTMGVITLVLIASQ
                     LQAPGTGPHIWVITACALAIAGGTYAGGWRIIRTLGSGLTDVKPAQGFAAETSTAAAI
                     LASSHLGFALSTTHVASGSVIGSGLGRKGSTVRWGAIGKISLGWLFTLPAAAVVGGLA
                     ALLIRTGTVGVVIVAILGLGA"
BASE COUNT          262 a          408 c          503 g          204 t
ORIGIN      
        1 ggcgcccagg cccaggatgg ccacgatgac gacgcccacg gtgccggtcc ggatcaagag
       61 ggcggcgagt ccgccgacca ccgccgcggc gggcagtgtg aacagccagc cgaggctgat
      121 cttgccgatg gcgccccacc ggacggtgga gcccttgcgg cccaggccgg agccgatgac
      181 ggagccggat gcgacgtgcg tggtggagag tgcgaagccg aggtggctgg aggcgaggat
      241 ggcggcggcc gtgctggttt ccgcggcgaa tccctgggcc ggcttcacgt cggtgagccc
      301 ggagccgagg gtgcggatga tgcgccagcc gccggcgtac gtgccgccgg caatggcgag
      361 ggcgcaggcc gtgatgaccc agatgtgggg tccggtgccc ggggcctgca gctgggaggc
      421 gatgagcacc agggtgatga cacccatggt cttctgggcg tcgttggtgc cgtgggccag
      481 cgccacgagg gatgagctga acacctggcc gtgcctgaag ccgccacgtt tctgggtgag
      541 cttgtctccg gaatcggggt cggagcggcg cgtgatggcg taggccatcc tggtgcaaag
      601 gtaggccacg ccaccggcga cggcgggtgc caacaacgcc gggatgatca ccttggacag
      661 gaccaccagg aagttgacgg cgccgaagga ccagctgcca actatggcgg ccccgatcag
      721 cccgccaaac agggcatgcg aggaactgga aggcagcccc agcagccagg tgaacaggtt
      781 ccacagcacg gcgcccatga gcccggcaaa gatcatctcc ggggtgatgg cgacgccgcc
      841 ggggccctca ttgatgatgc cgcccgagat ggtcttggcg acctccgtcg agaggaaggc
      901 ccccaccagg ttcaggatgg cggccagggc cacggccgtc ttgggcttga tggcaccggt
      961 ggcgatgggg gttgccatgg cgttggcggt gtcgtgaaag ccattcgtga agtcaaagaa
     1021 gagggccagt gcaatgacaa gcacgaccat gaaggtgaga tccacccgcg atccatttct
     1081 attgagggca aaagcgaagc ttcaaagagt ctagtcggac acggacgcca gcggacccgc
     1141 acgccctgca tggaggtgcc ccttgaacag aactaacctt acgatcctac gactgttttc
     1201 ctgagcacag gaattgtgga aagggcattt cccgcccggt ggccgcccac acgggaggcg
     1261 gccgcgctac gctacatgcc atggggcagg aatggtcccc gccgttgggt gccgctgcgg
     1321 acaatgtccg gttgagagga tggatggaac gctgtgagta acttggagac atcccca
//
LOCUS       DASTJJ010000201         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_886524, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000201 DASTJJ010000000
VERSION     DASTJJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886524"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..>1594)
                     /locus_tag="VFR02_02445"
     CDS             complement(249..>1594)
                     /locus_tag="VFR02_02445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9869346.1"
                     /translation="LAFLGVVGALLTLPCPWGPCAGVCWVPTSTFNGYLGVDGLSIYF
                     KSVILLGTLFTLVMAFRYFESKQVRHRAEFLAILLFSAAGATSLAGSREIITLYVSLE
                     TLTISSFLLAAFLKKEKGSNEAGLKYLLMGAFSSALLLFGLSYLYGLTGTTMIAEMAK
                     RLADQPASAIEVLAVILVVAGLSFKISLAPFHMWTPDVYEGAPTPVTAYLSVVSKTAG
                     FAAFTRIFWGGLAAPAILPVWVGLFGVLAALSMVLGNLEALPQTNLKRLLGYSSIAQA
                     GYLAVGFLAGGELGMTALLFYLVAYLFANLSLFLVVGLVAARTGGERIVDFAGLWKRA
                     PFLGLTLAVGLFSLAGLPPFAGFAGKWYLFSAAVAKGYLWLALIGVLFSAVSLYYYLQ
                     IVRQALLADPADASPIPVSWLEKAFLGLSVAALILFGVWPTPIAQAAQAAAATLF"
BASE COUNT          365 a          536 c          506 g          187 t
ORIGIN      
        1 caattccacc acctggaagc ccaggcccac ccccagccag cggaaggccg acgcctcccg
       61 ggcggcgtca aaatccgcca ggtagcccaa cgcccgccaa cagcctgcgc cataggccgc
      121 gacggccgcg gccaggatga agttcaaccc gttcatggga cctcctggaa ggtgggaaag
      181 gggcaaataa aaaccccggc catcctagcc ggggccgggg tacttttcca gcatcgcggc
      241 cggtgggatc agaagagggt cgcggcggcg gcctgggcgg cttgcgcgat gggcgtgggc
      301 cagaccccga agaggatcag ggccgccacg ctcaacccca ggaaggcctt ttccagccaa
      361 ctcacgggga tgggcgaggc gtccgccgga tcggccaaca aggcctggcg gacgatctgc
      421 aggtaataat aaagggagac cgcgctgaac agcaccccga tcagggccag ccaaagatag
      481 cccttggcca cggcggcgct gaagaggtac cacttccccg cgaaaccggc gaagggcggc
      541 aaacccgcca acgagaaaag gccgacggcg agggtcaaac ccaggaaggg cgcccttttc
      601 cacaggcccg cgaagtccac gatgcgctca ccgcccgtcc tcgcggccac caaccccacc
      661 accaggaaca agctcaagtt ggcgaacaga taagccacca gatagaacaa cagggccgtc
      721 atgcccagct cgccgccggc caggaagccc acggccaggt agccggcctg ggcgatggag
      781 gaataaccca ggagccgctt gaggttggtc tggggcaacg cttccaggtt ccccaacacc
      841 atgctgagcg ccgccaagac gccgaaaaga ccgacccaaa cgggcaggat ggccggcgcc
      901 gccaggccgc cccagaagat ccgcgtgaag gccgcgaacc ccgccgtttt ggagaccacc
      961 gacaggtagg cggtcacggg cgtgggggcg ccctcataaa cgtccggcgt ccacatgtgg
     1021 aagggcgcca gggatatctt gaaggacaag ccagccacga ccaggatcac cgccaacacc
     1081 tcgatggcgc tggccggctg gtcggccaaa cgcttggcca tttcggcgat catcgtggtg
     1141 cccgtcaggc cgtaaagata agagaggccg aacaacaaca gggccgagga aaaggccccc
     1201 atcaacaggt atttcagccc cgcctcattg ctcccttttt ccttcttcag gaaggccgcc
     1261 agcaggaagc tgctgatcgt cagggtctcc agcgacacat agagggtgat gatctcccgg
     1321 gacccggcca ggctggtcgc cccggcggcg gaaaaaagca ggatcgccag gaactcggcc
     1381 cggtgccgga cctgcttgga ctcgaaatag cggaaggcca tgaccagggt gaagagcgtc
     1441 cccagcagga tgaccgactt gaagtagatc gacaagccgt ccacccccag gtatccgttg
     1501 aaggtcgacg tcgggaccca acagaccccc gcgcaggggc cccagggaca gggcagggtc
     1561 agcagcgccc ccaccacgcc caggaaggcc aggc
//
LOCUS       DASTJJ010000202         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_641565, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000202 DASTJJ010000000
VERSION     DASTJJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641565"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1370
                     /locus_tag="VFR02_02450"
     CDS             <1..>1370
                     /locus_tag="VFR02_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET9869347.1"
                     /translation="AACVVGDGEAETGPLEASWKAPAFLNPSRDGAVLPILHLNGWKI
                     SGPTVLGRQSDGQVLALLRAHGWDPVVVSGSDPAVVHPQLAKALEDAHSGILAIQKQA
                     RRHGVSGPARWPAIILRTPKGWTGPREVDGMQVEGTFRAHQVPLTGVRENPAHLAQLE
                     SRLLSYKPETLFDDAGRLVPELAALVPQGKLRMGANPAANGISGSAPLPLRPLDLYAL
                     DVPAPGAVKHQTTRPLGEMFRDIYTDTARDPRFRLFCPDETNSNRLGAVFEATDRCLM
                     GPSREGDDHVSPHGRVMEVLSEHLCQGWLEGYLLTGRHGFFASYEAFAMVSASMTVQH
                     VKWLQHARELPWRRPVPSLNILLTSTCWRNDHNGFSHQGPGLIDTVLSLSGSVVRVYL
                     PPDSNTLLATAAHVLSSKDHVNLIVVDKQVHPQYLNLDDARAHAAAGASVWSWAGNAG
                     AAEPDIV"
BASE COUNT          233 a          483 c          425 g          229 t
ORIGIN      
        1 gcggcctgcg tcgtgggcga cggcgaagct gaaaccgggc ccctggaagc ttcgtggaag
       61 gcgccggcat tcctgaatcc cagccgggac ggggccgtgc tgcccatcct gcacctcaac
      121 ggctggaaga tttccggacc caccgtgctc ggccgacagt ccgacgggca ggtcctggcc
      181 ctgctccggg cacacggctg ggaccccgtc gttgtttccg gcagcgaccc cgccgtggtc
      241 cacccgcaac tggccaaggc cctcgaggac gcccattccg ggattctcgc aatacaaaaa
      301 caagcgcgtc ggcatggcgt gagcggcccg gcacgctggc cggcgatcat cctgcgcacg
      361 cccaaggggt ggaccggccc acgtgaagtg gatggcatgc aggtggaagg caccttccgc
      421 gcccaccagg tgccgctaac cggtgtgcgg gagaatcccg cccacttggc ccagctggag
      481 tcccggctgc tctcctacaa accggaaacc ctgtttgacg acgccgggcg gctggtccca
      541 gaactggctg ccctcgtccc gcagggaaag ctgcgcatgg gcgccaaccc cgcggcgaac
      601 ggcatcagcg gcagcgcccc gctgccgctg cgtcccctcg atctttacgc tcttgacgtg
      661 cccgctccgg gggccgtcaa gcaccaaacc accaggccgc tgggcgaaat gttcagggac
      721 atctacacgg ataccgcccg agacccccga ttccggctct tctgccccga tgagaccaac
      781 agcaaccggc tcggcgctgt tttcgaagcc accgatagat gcctcatggg accgtcccgg
      841 gaaggggatg accacgtttc cccgcacggt cgggtcatgg aggtcctttc cgagcaccta
      901 tgccagggat ggctggaggg gtacctgctg acggggcggc atgggttctt tgccagctat
      961 gaggcttttg ccatggtgag tgcgtcgatg accgtccagc atgtcaagtg gctccagcat
     1021 gcccgggaac tgccgtggcg caggcccgtt ccaagcctga acatcctcct gacctccacc
     1081 tgttggcgca acgaccataa cggcttcagc catcaaggtc cgggcctgat tgacaccgtg
     1141 ctgtcgcttt ccggttccgt ggtccgcgtc tacctgccac cggactccaa caccttgctc
     1201 gccaccgcgg cccacgttct ctcgagcaag gaccatgtga acctgatcgt cgtggacaag
     1261 caggtccacc cgcagtacct gaacctcgac gacgcccggg cacatgccgc tgccggtgcc
     1321 tccgtctggt cctgggccgg gaacgctgga gcagccgagc cggacattgt
//
LOCUS       DASTJJ010000203         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_152931, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000203 DASTJJ010000000
VERSION     DASTJJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152931"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..349)
                     /locus_tag="VFR02_02455"
     CDS             complement(11..349)
                     /locus_tag="VFR02_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET9869348.1"
                     /translation="MAKKLEVLRVYLDEQWKWKGNLLYRALVEELRRQGASGATVFRA
                     VEGFGSSVKIREAHILEMSENLPLVLEVAETPAQIRKLWKVLSPFLPAKCLATIHSVR
                     ALSPGRPKGR"
     gene            complement(360..734)
                     /gene="crcB"
                     /locus_tag="VFR02_02460"
     CDS             complement(360..734)
                     /gene="crcB"
                     /locus_tag="VFR02_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET9869349.1"
                     /translation="MEKLLWISLGGVLGAWSRYYVGLWAADKWGTAFATGTFLINFLG
                     SAFMAWILTLSLEKGLFTPHLRLALTVGFCACFTTFSTFSWDTFRYLSEGNWKYALLN
                     VGLNVGGCLIGTWSGVVLARAW"
     regulatory      complement(804..867)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(909..>1314)
                     /locus_tag="VFR02_02465"
     CDS             complement(909..>1314)
                     /locus_tag="VFR02_02465"
                     /inference="COORDINATES: protein motif:HMM:NF003975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HET9869350.1"
                     /translation="YGLHYDNAVGPAQGRSGLMRADLSATLFLSDPADYEGGELSIED
                     TYGFHQVKLPAGHLVVYSAGALHEVKPVTRGRRVACFFWIQSLVADEQERSILSDLDN
                     ALAVLSKDHPQHPAVSALTRGYNHLLRKWVTV"
BASE COUNT          241 a          444 c          422 g          207 t
ORIGIN      
        1 cggcggcctt tcagcggcct ttgggacggc cgggggaaag ggccctcacc gaatggatgg
       61 tggccaggca tttggccggc aggaagggcg agagcacctt ccagagcttg cggatctggg
      121 cgggcgtctc cgccacctcc agcaccaggg gcaggttctc gctcatttcc aggatatggg
      181 cctcgcggat cttcaccgag gacccgaagc cctccaccgc gcggaagacc gtggcgcccg
      241 aggccccctg ccgccgcagt tcctccacca gggcccggta caggaggttc cccttccatt
      301 tccactgctc gtccaggtac acccgcaaca cttccagttt cttggccatg gcgccctcct
      361 taccaggccc gggccaggac aacgcccgac caggtgccga tcaaacaacc gcccacgttc
      421 agccccacgt tcaacagggc gtacttccaa ttcccctccg acaggtagcg gaaggtgtcc
      481 caactgaagg tcgaaaaggt ggtgaaacag gcgcagaagc ccaccgtgag cgccaggcgc
      541 aggtgggggg tgaagaggcc cttttccagg ctcagggtca ggatccaggc catgaaggcc
      601 gaccccagga aattgatgag gaaggtcccc gtggcgaagg ccgtgcccca cttgtcggcg
      661 gcccagagcc ccacgtagta gcggctccag gcgcccagca cgccccccag ggagatccac
      721 agcagttttt ccatcgcgct attcctttcc gccggtgaag ggggcgaaac aaaaaaaccc
      781 ctaccgacag gggcttggcc ccggattcgg taggagtcat cagccttcag gaaggcggtt
      841 gcggcggact ccatcgcctt aagttcgggc ccattctagc ccggcgggtc cccgcccgca
      901 agcggaccct agaccgtgac ccacttgcgc agcaggtggt tgtagccccg ggtcagggcc
      961 gacacggccg gatgctgggg atggtccttg ctgaggaccg ccagggcgtt gtccaggtcc
     1021 gacaggatgg accgttcctg ttcgtcggcc accaggctct ggatccagaa aaagcaggcc
     1081 acccgtcggc cgcgggtcac gggcttgacc tcgtgcaggg cccccgccga atagaccacc
     1141 aggtggcccg ccggcagctt cacttggtgg aaaccgtagg tgtcctcgat gctcaactcc
     1201 ccgccctcgt agtcggcggg gtcggacagg aaaagggtgg ccgataggtc cgcccgcatg
     1261 agcccggacc ggccctgggc ggggcccacg gcgttgtcgt aatgcaggcc atag
//
LOCUS       DASTJJ010000204         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_886713, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000204 DASTJJ010000000
VERSION     DASTJJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886713"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1955
                     /gene="lepA"
                     /locus_tag="VFR02_02470"
     CDS             156..1955
                     /gene="lepA"
                     /locus_tag="VFR02_02470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET9869351.1"
                     /translation="MSLDHIRNFCIIAHIDHGKSTLADRLLEFTGAIPPREMRDQLMD
                     QMDLERERGITIKAKAVRFFYKAKDGRTYEMNLIDTPGHVDFNYEVSRSLKACEGALL
                     LIDASQGVEAQTLANAFLAFDQNLAIVPVINKIDLPAADPERCKRQMEESLALPGEDA
                     ILASAKEGLGIEDILEAVVRVVPPPKGEAAEPLKGLIFDSMFDIYRGVIVFVRLLNGV
                     MQPGTKMQFMSTGKVFEVEEVGVFKPKMQKVDKLSAGEVGYVCANIKEVRDAKVGDTV
                     TEEGRPTAQALPGFKDVLPMVFCGLYPVNSEDYSDLKESLSKLKLSDAALSYEPETSA
                     ALSFGFRCGFLGLLHMEIVQERLEREYNLNLIATAPSVVYRVLKTDGGVLTVDNPSKL
                     PDPSLIEEIDEPYIDAKIIVPADYIGAVMTLCQERRGLHKDTKFLDTTRAVITYELPL
                     AEVVMDFFDKLKSCTKGYASLDYEPMGVKPGNVVKVDILLNGEPVDALSFMVHRDKAE
                     VRGRSIAEKLKEIIPQQLFEVPIQAAIGGKIIARETVRARRKDVLAKCYGGDISRKRK
                     LLEKQKEGKKRMKSVGSVEIPQEAFMVVLKVED"
     gene            1986..>2371
                     /gene="lepB"
                     /locus_tag="VFR02_02475"
     CDS             1986..>2371
                     /gene="lepB"
                     /locus_tag="VFR02_02475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9869352.1"
                     /translation="MNIDWADNLNAILYGMAIFLTAVELFMRLVWPRLRGRAPAGLFK
                     ASQPGGVMETIDSLWVAMVVALTLKALLIQPFTIPSGSMEDTLQVGDYILVKKFEYGY
                     SLLNKSPRFLEFHRPKRGDVVVFVFPG"
BASE COUNT          450 a          772 c          741 g          408 t
ORIGIN      
        1 cgaattttat tgaggaatgc cgggttttgg ggcgccgggc cgagaggccc ggcgcctttt
       61 tttatgggcc gggccgccct ttcccgcctt caatttccaa cccgcctgcc ctatactgac
      121 cgcctttgcc gaagcccctc aaggagcctc atcccttgtc gctcgaccac atccgcaatt
      181 tctgcatcat cgcccacatc gaccacggga aaagcaccct ggcggaccgg ctgctcgaat
      241 tcacgggcgc catcccgccc cgggagatgc gcgaccagct gatggaccag atggacctgg
      301 aacgggaacg gggcatcacc atcaaggcca aggccgtgcg gttcttctac aaggccaagg
      361 acggccgcac ctacgagatg aacctcatcg acacgcccgg ccacgtggac ttcaactatg
      421 aggtgtcccg cagcctcaag gcctgcgagg gcgcgctgct cttgatcgac gcctcccagg
      481 gcgtggaggc ccagaccttg gccaacgcct tcctggcctt cgaccagaac ctggccatcg
      541 tgcccgtcat caacaagatc gacctgcccg cggccgaccc ggagcgctgc aagcgccaga
      601 tggaggaatc cctggccctg cccggggagg acgccatcct ggcctcggcc aaggaaggcc
      661 tgggcatcga ggacatcctg gaggccgtgg tgcgcgtggt gccgccgccc aagggcgaag
      721 ccgccgagcc cctgaagggg ctcatcttcg attccatgtt cgacatctac cggggcgtca
      781 tcgtgttcgt gcggctcctg aacggcgtca tgcagccggg caccaagatg cagttcatgt
      841 ccaccggcaa ggtcttcgag gtggaggaag tgggcgtctt caagcccaag atgcagaagg
      901 tggacaagct ttccgcgggc gaagtgggct atgtctgcgc caacatcaag gaagtgcgcg
      961 acgccaaggt gggggacacg gtcaccgagg aggggcgccc cacggcccag gccctgccgg
     1021 gcttcaagga cgtgttgccc atggtcttct gcgggctcta cccggtgaac tcggaggact
     1081 attcggacct gaaggaatcg ctgtccaagc tcaagttgtc cgacgcggcg ttgtcctacg
     1141 agcccgagac ctcggcggcg ctttccttcg ggttccgctg cggtttcctg gggctgctgc
     1201 acatggagat cgtgcaggag cgcctggaac gcgagtacaa cctcaacctc atcgccaccg
     1261 cccccagcgt ggtctaccgg gtgctcaaga ccgacggcgg cgtattgacc gtggacaacc
     1321 cctccaagct gcccgacccc tccctgatcg aggagatcga cgagccctac atcgacgcca
     1381 agatcatcgt gcccgcggac tacatcggcg ccgtgatgac gctgtgccag gaacgccggg
     1441 gcctgcacaa ggacaccaag ttcctcgaca ccacccgggc cgtcatcacc tatgagctgc
     1501 ccctggccga ggtggtcatg gacttcttcg acaagctcaa gagctgcacc aagggctacg
     1561 cctcgctcga ctatgagccc atgggggtca agccgggcaa cgtggtgaag gtggacatcc
     1621 tcctgaacgg cgaaccggtg gacgccctgt ccttcatggt gcaccgggac aaggccgagg
     1681 tccggggccg gtccatcgcg gaaaagctca aggagatcat cccccagcag ctcttcgagg
     1741 tgcccatcca ggcggccatc ggcggcaaga tcatcgcccg ggagaccgtg cgggcccgcc
     1801 gcaaggacgt gttggccaag tgctatggcg gtgatatttc gcgcaaacgc aagcttttgg
     1861 agaaacaaaa agaaggcaag aagcgcatga agtcggtcgg cagcgtcgaa atcccccagg
     1921 aagccttcat ggtggtgttg aaggtggagg actaggccgg cgccggacaa ccccgaggag
     1981 accttatgaa catcgattgg gcggacaacc tgaacgccat cctgtacggg atggcgattt
     2041 tcctgaccgc ggtggaactt ttcatgcggc tggtctggcc gcgcctgcgg ggccgggcac
     2101 cggccggcct cttcaaggcc agccaacccg gcggcgtgat ggagaccatc gactcgcttt
     2161 gggtggccat ggtggtggcc ctgaccctga aggcgctgct gatccagccc ttcaccatcc
     2221 cctcggggtc catggaggac acgctccagg tcggcgacta catcctggtg aagaagttcg
     2281 agtacggcta ttcgctgctc aacaagtcgc cccggttcct cgaattccac cggcccaagc
     2341 gcggcgacgt ggtggtcttc gtcttcccgg g
//
LOCUS       DASTJJ010000205         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1252972, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000205 DASTJJ010000000
VERSION     DASTJJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252972"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFR02_02480"
     CDS             <1..807
                     /locus_tag="VFR02_02480"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET9869353.1"
                     /translation="KMADDMAARTNKVCWDGDWYAMAFDGWGTMLGSKKQKDGKVYLN
                     TQTWAVISGVADAKRGPACMDAVKEYLDSKYGVALMYPGYKHFSPRLGGVSTFPPGLK
                     ENGGIFSHANPWAVIAETKLGRADIAMKYYKQLLPPTKDRIQDVHKAEPYIYAQAIAS
                     REHRDFGFARNSWLTGAASWNYVAATQYILGVRPTYRGLAVEPCIPKDWSQYEVTRDF
                     RGATYHITVKNPRRVSGGVRSIAVNGKRVAGTVIPAQKAKGRYEVEVILG"
     gene            912..1178
                     /locus_tag="VFR02_02485"
     CDS             912..1178
                     /locus_tag="VFR02_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HET9869354.1"
                     /translation="MTLKVAAKSNPSTVAGAIANNVRDGKQVEIVAMGPESINNTVKA
                     IAIARGFLHGDGMDASCKPEFIHLTIDGEKKSAIKFLLAIEKGV"
     gene            1241..1317
                     /locus_tag="VFR02_02490"
     tRNA            1241..1317
                     /locus_tag="VFR02_02490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1275..1277,aa:Val,seq:tac)
     gene            1501..>2238
                     /locus_tag="VFR02_02495"
     CDS             1501..>2238
                     /locus_tag="VFR02_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869355.1"
                     /translation="MRFQIFLAFLRSSFIPAIALAAPALGAGPSAPATLVFEGQDLLV
                     LRSPILSFTPADRTAAISAKLHRFATDPLFDPSTLAAEPGDGSTAITAGDVVLMSVTD
                     ADARDAGLKREALADRDLALFRRTLSESHLRYGPRAFVKGGLETLALTLVLLGILAGL
                     RWVHHKAWGAVEGARTLLASRLGIQRLQLLSPRQAEDLVKGLLNLMRAAASLFFFYVD
                     LSLVLGFFPWTASFSGRLFGYFWSPLGA"
BASE COUNT          383 a          758 c          725 g          372 t
ORIGIN      
        1 aagatggccg acgacatggc cgcccgcacc aacaaggtct gctgggacgg ggattggtac
       61 gccatggcct tcgacggatg gggcaccatg ctgggctcca agaagcagaa ggacggcaag
      121 gtctacctga acacccagac ctgggccgtc atctcgggcg tggccgacgc caagcgcggg
      181 cccgcctgca tggacgcggt caaggaatac ctggattcga agtacggcgt ggccctgatg
      241 tacccgggct acaagcactt cagcccccgc ctgggcggcg tcagcacctt cccgccgggc
      301 ctgaaggaga acggcggcat cttctcgcac gccaatcctt gggcggtcat cgccgagacc
      361 aagctgggcc gggccgacat cgccatgaag tactataagc agctgttgcc ccccaccaag
      421 gaccgcatcc aggacgtgca caaggccgag ccctacatct acgcgcaggc catcgcctcg
      481 cgggaacacc gggacttcgg cttcgcgcgc aactcctggc tgacgggcgc ggccagctgg
      541 aactacgtgg ccgccaccca gtacatcctg ggcgtgcgcc cgacctaccg gggcctggcc
      601 gtcgagccct gcatccccaa ggattggagc cagtacgagg tgacccgcga cttccgcggg
      661 gcgacctacc acatcaccgt gaagaacccc cgccgcgtga gcggcggcgt gaggtcgatc
      721 gcggtgaacg gcaaacgcgt ggccggcacg gtgatcccgg cgcaaaaggc caagggccgt
      781 tacgaagtcg aagtgatcct cggttaaacg gcccctgggg gccgttcggt tgacagcccc
      841 caggccccgt gttataaaaa gtccggtaca atggcagggt cggtaagaag gagccacgcc
      901 acgtggggat catgacgctc aaagtcgcag ccaaaagcaa tccttccacg gtggccgggg
      961 ccattgccaa caatgtccgg gacgggaaac aggttgagat cgtggccatg gggcccgaga
     1021 gcatcaacaa cacggtcaag gccatcgcca tcgcccgggg tttcctgcac ggcgacggca
     1081 tggacgccag ctgcaaaccc gagttcatcc acctgaccat cgacggcgag aagaagagcg
     1141 ccatcaagtt cctcctggcc atcgaaaagg gcgtctgacc ctcccgcacc catctttccg
     1201 catcccagga ccctggccat cgggccgggg ttttgttttt gggcgaatag ctcagctggt
     1261 tagagcacct gctttacacg cagggggtcg taggttcgaa ccctacttcg cccaccagtc
     1321 tccgccccga tggggctacg cctggcaagc ccgtctttgg cgacccgaaa gggggaccgt
     1381 ccgaagcggg gactggccgg cgtagttccc gacgagggaa cgaagcgggg acggggttag
     1441 agcttgatgg ggttccttca aaaaatcacc cggcgatgct gggtaggtcg agggggattc
     1501 atgcggttcc aaattttcct agccttcctg cggtcttcct tcatccccgc catcgccctg
     1561 gcggcccccg cccttggggc cgggccctcg gccccggcca ccctggtctt cgaggggcag
     1621 gacctgctgg tcctccggtc gcccatcctg tccttcaccc cggcggaccg gaccgcggcc
     1681 atctcggcca agctccatcg gttcgccacc gaccccctgt tcgaccccag caccttggcg
     1741 gccgaaccgg gggacggctc gacggcgatc acggccgggg acgtggtcct gatgtcggtc
     1801 accgacgccg acgcccggga cgccgggttg aaacgggaag ccttggcgga ccgggacctg
     1861 gccctgttcc gccgcacgct gtccgagagc cacctgcgct acggcccccg ggcctttgtg
     1921 aagggcggcc tggagaccct ggcgctcacc ctggtgctgc tcggcattct ggcggggctg
     1981 cggtgggtcc accacaaggc ctggggcgcc gtggagggcg cgcgcaccct gctggcttcc
     2041 cggttgggca tccagcggct gcagctgctt tcgccccgcc aggcggagga cctggttaag
     2101 ggcctgctca acctcatgcg agccgcggcg tctctgttct tcttctacgt cgacctgtcg
     2161 ctggtgctgg gtttcttccc ctggacggcc agtttttcgg ggaggctctt cggctatttc
     2221 tggtcgccgc tgggcgcc
//
LOCUS       DASTJJ010000206         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_886819, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000206 DASTJJ010000000
VERSION     DASTJJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886819"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFR02_02500"
     CDS             complement(<1..935)
                     /locus_tag="VFR02_02500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9869356.1"
                     /translation="MARRAAGKKGRATWAWLLWPALGLALLLSQWARLRYPFTDNEGC
                     LAHMARQWAFGRGLYADVWSNKPPLFFLPAYLLQQAFTLNETTLHLYSLSLHALDALA
                     LFALARRWGWGPGAWVTAFAYAALIVPPLFLPWSAESDLMMQPFLLWAFWAAQGKTPG
                     GAWLAGLLWGLAFLTKQSAVFFLPAGLAALGWKEWPRLLRAGGGAASALALGMGPFFL
                     KGEGLLALRDMIGSNSGYAPGGWRFLLHAPWYRGFLARWLGWVAAVYGGFFAVFLWNW
                     RKARRGDPLPLLALWALGALAAACASGYFFSYYFIS"
     gene            complement(938..>1368)
                     /locus_tag="VFR02_02505"
     CDS             complement(938..>1368)
                     /locus_tag="VFR02_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869357.1"
                     /translation="ALADGASKASHEIADLVQQIQDQTLATVGSMEKSAQEAEQSRGG
                     IRVMGDALNDIIRVIEDVEGQSRAITELVAQQTQRYNQIVNGIQDINAVSEESAASTQ
                     EVSASTQEQTASLEQVTATCRELAHMAQDMKLMVEKFKVR"
BASE COUNT          248 a          460 c          469 g          191 t
ORIGIN      
        1 gagatgaagt aataggaaaa aaaatagccg ctggcgcagg cggccgccag ggcccccaag
       61 gcccaaaggg ccaggagcgg caggggatcc ccccggcgcg ccttgcgcca gttccagagg
      121 aagaccgcga agaaaccgcc gtaaacggcc gcgacccaac ccagccaacg cgccaggaag
      181 ccccggtacc aaggcgcgtg gagcaggaac ctccacccgc ccggggcgta gcccgaattg
      241 gaaccgatca tgtcccgcaa cgccaacagg ccctcccctt ttaaaaagaa ggggcccatc
      301 cccagcgcca aggccgacgc cgcgccgccg cccgcccgca gcagccgggg ccattccttc
      361 caacccagcg ccgccaggcc cgccggcagg aaaaagacgg cgctttgttt ggtcaaaaag
      421 gcgaggcccc agagcaggcc cgccagccac gccccgcccg gggtcttccc ttgggccgcc
      481 caaaaggccc aaagcaggaa tggctgcatc atcaggtccg actcggcgga ccagggcagg
      541 aaaaggggcg gcacgatcag cgccgcgtag gcgaaggccg tcacccaggc gcccggcccc
      601 caaccccagc ggcgggccaa ggcgaagagg gccagcgcgt ccaacgcgtg gagcgacagg
      661 ctgtaaaggt gcagggtggt ctcgttgagg gtgaaggcct gctgcaacag gtaggcgggc
      721 aggaagaaga gcgggggctt gttgctccag acgtccgcgt agaggccccg gccgaaggcc
      781 cattggcggg ccatgtgggc caggcaaccc tcgttgtcgg tgaagggata gcgcaggcgg
      841 gcccattggg aaagcagcag ggccagcccc agggccggcc agaggagcca ggcccaagtg
      901 gcgcggccct ttttcccggc ggcccgcctg gccaaggtca gcggaccttg aacttttcca
      961 ccatcagctt catgtcctgg gccatgtggg ccagttcccg gcaggtggcg gtgacctgtt
     1021 ccaggctggc ggtctgttcc tgggtggagg ccgagacctc ttgggtggag gcggcggatt
     1081 cctcgctgac cgcgttgatg tcctggatgc cgttgacgat ctggttgtag cgctgggtct
     1141 gctgggccac cagctcggtg atggcgcggc tctggccctc cacgtcctcg atgacgcgga
     1201 tgatgtcgtt caaggcgtcg cccatgaccc ggataccgcc ccggctctgt tcggcttcct
     1261 gggcgctctt ttccatcgac cccacggtgg ccagggtctg gtcctggatc tgctgcacca
     1321 ggtcggcgat ctcatgggag gccttgctgg ccccgtcggc cagggccc
//
LOCUS       DASTJJ010000207         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_641931, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000207 DASTJJ010000000
VERSION     DASTJJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641931"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /gene="rpoN"
                     /locus_tag="VFR02_02510"
     CDS             <1..1209
                     /gene="rpoN"
                     /locus_tag="VFR02_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET9869358.1"
                     /translation="RMTQETWTLYGEGEEYGDYYGGQGGNATAEDQEKHDFLEASITR
                     QDTLAEHLEWQLLLAAKTEEEKRLGRVLIGNLDDNGYLTDPLEELARAQGVAPHDLER
                     VLQLIQTFDPPGVGARTLKECLLLQLENRTGEAARKAYRLVAEHFEDVEKRRYPVIQK
                     KMELGADELRALLAFVATLEPKPGRSFQENNTKYVTPDVSVRKIEGEYVVVLNDEPVP
                     RLRISSYYRRLLKDKEALKTKETKAFLDNKIQSAMWLIKNIEQRRKTIYRVTEAIFRI
                     QRDFLETGLSGLKPLTLKQVADMIGMHESTVSRVTTHKYVQTPRGLFELKYFFTSGLE
                     SEAGLDVSSLSVKEKIKELVDQEPPQRPHSDQKLTQLLKDQGIFIARRTIAKYREELR
                     IPPASQRRKY"
     gene            1345..1881
                     /gene="raiA"
                     /locus_tag="VFR02_02515"
     CDS             1345..1881
                     /gene="raiA"
                     /locus_tag="VFR02_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9869359.1"
                     /translation="MKIIITSRHEKLTEALRASVEDKIKRVERYLGKIKEAHVVLNFE
                     KKGHTCEINLTAKGLKMSSKAVSRDMYNSIDGALEKLETQAKKHKEIRVERHHGRVSP
                     FKSVTHGLIPPPQEGEGIEVIQSRKYSVKPMAVEEAALQLAKSKDEFMVFFDAETGRT
                     GVVYKRKDHHIGLIEPEY"
     gene            2006..2470
                     /locus_tag="VFR02_02520"
     CDS             2006..2470
                     /locus_tag="VFR02_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9869360.1"
                     /translation="MQILDFLTADAIKLNLESKTKKDVIKEMVDLLVKAGKVKDKKKM
                     LQTLLEREELGSTGIGQGIAIPHGKSDTVSDLAAAFGVSQEGLSFDSLDGEPVNLFFL
                     LVAPEGAAGAHLKALARISSLLKDKYFRKSLLSAKSPDEVLKIIQEEEKLKH"
BASE COUNT          584 a          846 c          790 g          408 t
ORIGIN      
        1 cggatgaccc aggagacctg gaccctctac ggcgaaggcg aggaatacgg cgactattac
       61 gggggccagg gcgggaacgc caccgccgag gaccaggaaa aacacgattt tctcgaggcc
      121 tccatcaccc gccaggacac cctggccgag cacctggaat ggcagctgct cctggcggcc
      181 aagaccgagg aggaaaaacg cctgggccgg gtgctcatcg ggaacctgga cgacaacggc
      241 tacctgaccg atccgctgga ggaattggcc cgcgcccagg gggtggcccc ccacgacctg
      301 gaacgggtgc tgcagctcat ccaaaccttc gacccgccgg gggtgggcgc ccggaccctc
      361 aaggaatgcc tgttgttgca gttggagaac cggaccgggg aggccgcccg caaggcctac
      421 cgcctggtgg ccgagcattt cgaggacgtg gaaaaaaggc ggtatccggt catccaaaaa
      481 aagatggagc tcggcgccga cgaactgcgc gccctgctgg ccttcgtggc cacgctggaa
      541 cccaagcccg gccggtcctt ccaggagaac aacaccaagt acgtcacccc cgacgtgagc
      601 gttcgcaaga tcgaggggga atacgtggtg gtgctcaacg acgagcccgt gccgcgcctg
      661 cgcatctcat cctattaccg gcgcctgctc aaggacaagg aggccctcaa gaccaaggag
      721 accaaggcct tcctggacaa caagatccaa tccgccatgt ggctcatcaa gaacatcgag
      781 cagcggcgca agaccatcta ccgggtgacc gaggcgatct tccgcatcca gcgcgacttc
      841 ctggagacgg gcctgagcgg gctcaagccc ctcaccctca aacaagtggc cgacatgatc
      901 ggcatgcacg agtccaccgt cagccgggtg accacccaca agtacgtgca gacgccccgg
      961 ggcctcttcg agctgaagta tttcttcacc agcggcctgg aatcggaggc ggggctggac
     1021 gtgtcgtcgc tttcggtgaa ggagaagatc aaggaactgg tcgaccaaga gccgccccag
     1081 aggccccatt cggaccagaa gctgacccaa ctgctgaagg accagggcat cttcatcgcc
     1141 cggcgcacca tcgccaagta ccgcgaggaa ctgcgcatcc ccccggcctc ccaacgccgc
     1201 aaatattgac cccgccccgg gccttgcctc cccgtttatc cccgcctttt cgaccttttt
     1261 caccgccgcc tgttcattga cttcccccct tcgggtgtta gaataatccg gtttttcagc
     1321 actttcacgc caccaggatc ccacatgaaa atcattatca ccagccgcca cgagaagctc
     1381 acggaagcgc tgcgcgcctc cgtggaagat aagatcaagc gggtggagcg ttatttgggc
     1441 aagatcaagg aagcccacgt cgtgctcaac ttcgagaaga agggccacac ctgcgagatc
     1501 aacctgacgg ccaagggcct gaagatgagc tccaaggccg tttcccgcga catgtacaat
     1561 tccatcgacg gggccctgga aaaactcgag acccaggcca agaagcacaa ggagatccgg
     1621 gtcgaacgcc accatggccg ggtgtcgccc ttcaagtcgg tcacccacgg cctgatcccg
     1681 cctccccaag aaggcgaggg gatcgaggtc atccagtcgc gcaagtactc ggtcaagccc
     1741 atggcggtgg aagaggcggc cctccaactg gccaagtcca aggatgagtt catggtcttc
     1801 ttcgacgccg agacgggccg caccggcgtg gtgtacaagc gcaaggacca ccacatcggc
     1861 ctcatcgaac ccgaatactg accccggccc cctttttccg gcgaaaagcc ccgaatccgg
     1921 cccttcggcc gggaaggcgg ccttacatcc ggggaagggg tcgtggtagg gtggccccaa
     1981 ggggcagccc ccaggaggat tttccatgca gatattggat tttttgaccg cggacgccat
     2041 caagctgaac ctggagtcca agaccaagaa ggatgtcatc aaggaaatgg tggacctgct
     2101 ggtcaaggcc ggcaaggtga aggataagaa gaagatgctg cagaccctgt tggagcgcga
     2161 ggaactgggc tccacgggga tcgggcaggg catcgccatc ccccacggca agtcggacac
     2221 ggtctcggac ctggcggcgg ctttcggggt ctcccaggaa ggcttgagct tcgactcgct
     2281 ggacggcgag ccggtcaacc tgttcttcct gctggtggcg cccgagggcg cggcgggcgc
     2341 ccacttgaag gccttggccc gcatttccag cctcttgaag gacaagtatt tccgcaaatc
     2401 cctcctttcg gccaagagcc ccgacgaggt cctcaagatc atccaagaag aagagaagct
     2461 gaagcactag ggcgacgccg gccgaatccc cgaaccggga gccgatcgat gaaaaaaata
     2521 tccgtcgaag ccctcctgca acaggccaag gaacggctgc gcctcacgcc cgtggtcgag
     2581 gcgggcatgg cgtcccggca catcgtgagc gtggacatca accgcctg
//
LOCUS       DASTJJ010000208         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_520160, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000208 DASTJJ010000000
VERSION     DASTJJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520160"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFR02_02525"
     CDS             <1..238
                     /locus_tag="VFR02_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9869361.1"
                     /translation="DLGGISAKVLTTRLRELEKRGVVTRSVMPTSPPTVEYALTALGG
                     KLNPVLDAIVEVGKQLPLPEGKPKKSAVPAALAH"
     gene            317..1738
                     /locus_tag="VFR02_02530"
     CDS             317..1738
                     /locus_tag="VFR02_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9869362.1"
                     /translation="MKKIIAAVGLGLVLGAGAARAQGSDGYYLTVYNDDLVLVGDERR
                     LPVQAGTHTMDFNDIAATLDPTSVNVDCPGDPGFRVLEQNYEYDLVNAQALLSRYLGR
                     TITLLHFGGEKAPDLSVKLLSADGGRLVVEKQDGSLLSVDPTADLQFPTLPEGLSFKP
                     SLRWRARSDRSGQEAVHLTYLATGMGWHADYTLLLNDREDGADFDGWVTLDNHTGTAF
                     PDAHLKLLAGEVNRVQAAPPREALFEAKAMAAPAPAFAEKALSDYHLYTLSRTTTLQN
                     NETKQIELLSAASVPVKKLYRYDGAGITDWWGNGITDRYYGNDSGTKKVDVFLKFTDD
                     QASGLGMPLPAGKIRVYQKDDDGNREFLGEDAIDHTPQDEQVLVRMGTAFDVVGERTQ
                     TAFTQPDAHSIEESFAITVKNHKKQAVQVDVVEHLYRYSNWKIVEKSQDFNKVDARTV
                     SFPVSVPAGGKATVTYKVRYWWN"
     gene            1839..1988
                     /locus_tag="VFR02_02535"
     CDS             1839..1988
                     /locus_tag="VFR02_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869363.1"
                     /translation="MFCRRCKVKMRELAHSAHKKRKFVCPKCGAARMMGGKSKASDRR
                     RLERY"
     gene            complement(2065..3084)
                     /locus_tag="VFR02_02540"
     CDS             complement(2065..3084)
                     /locus_tag="VFR02_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HET9869364.1"
                     /translation="MRNCAKVHPLLPLEAEGALTPGERKKVRAHLSACPAARAELESL
                     RRLRDVLRRQPEPPLPWDLHGKIMARLRKEGLGRTSGGWGRTLWPLAAAAATTVFFFT
                     ENPRWEEIVRSPNPPAAPQTPSEEVAAKKVTASIMKAPGPAALSNAAAPAPAMAPAAG
                     PPAAGAPPQAFEAKSVQAPRAAMDEASGGANAPEFSAQTAAQPHQAVRSLPAAQAMRN
                     GKTASWSGDQDPLSQTAGQSLITDEAAFEKVWRSLKPGGPVPPVDFTTQAVVFLEAGL
                     EPTPGYRVEVTAVEEQAGQVVVHWRTAPPPADAVLAQQVTYPWLLQAIPKPDNPVLFL
                     QDPGQ"
     gene            complement(3081..>3352)
                     /locus_tag="VFR02_02545"
     CDS             complement(3081..>3352)
                     /locus_tag="VFR02_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9869365.1"
                     /translation="DAEGDWLGRAPDKKTRQAPELMESKEFRKALEAALSGLPPDFKE
                     AVVLRDLEDLDYDRIAQMLRLGLGTVKSRIARGRALLREQLKDWL"
BASE COUNT          568 a         1116 c         1123 g          545 t
ORIGIN      
        1 ggacctgggc ggcatctcgg ccaaggtgct caccacgcgc ctgcgcgagc tggaaaagcg
       61 gggcgtggtg acccggtcgg tcatgcccac ctcgccgccc acggtggaat acgccctcac
      121 ggccctgggg ggcaagctca atccggtgct ggacgccatc gtggaagtgg gcaagcaact
      181 gcccctgccc gagggcaagc cgaaaaaaag cgccgtcccc gccgccttgg cccattgaat
      241 tttccccgct gctccgggaa ccttttggcc cggcgcgggt cccatccttc agaacgccgt
      301 tccaaggggg aaaaccatga aaaagatcat cgccgcggtg gggttggggc tggtgctggg
      361 cgcgggagcg gcccgggccc aggggtcgga tggctactac ctgacggtct ataacgacga
      421 tctggtcctg gtgggcgacg aacgccggct gccggtccag gccgggacgc acacgatgga
      481 cttcaacgac atcgccgcca ccctggaccc caccagcgtg aacgtggact gcccgggcga
      541 cccgggtttt cgggtgctgg aacagaacta cgaatatgac ctggtcaacg cccaggcgct
      601 gctttcccgc tacctgggcc ggacgatcac gctgctccat ttcggggggg agaaggcgcc
      661 cgacctttcc gtgaagctgc tttccgccga cggcgggcgc ctggtggtcg aaaagcagga
      721 cggcagcctt ctgtcggtgg accccaccgc cgacctgcag ttcccgaccc tgcctgaggg
      781 cctcagcttc aagccctcgc tcaggtggcg ggcgcggtcg gaccggtcgg gccaggaggc
      841 cgtccacctc acttacctgg ccaccggcat gggttggcac gccgactaca cccttttgct
      901 caacgaccgg gaggacggcg cggacttcga cggctgggtg acgctcgaca accacacggg
      961 caccgccttc cccgacgccc acttgaagct cttggccggg gaagtgaacc gggtccaggc
     1021 cgccccgccc cgggaggcgc tgttcgaggc caaagccatg gccgcgccgg cgcccgcctt
     1081 cgccgagaag gccctcagtg actaccacct ctacaccctg tcgcgcacca ccaccctcca
     1141 gaacaacgag accaagcaga tcgagctgct ctccgccgct tccgtgcccg tgaagaaact
     1201 ctaccgctat gacggggcgg ggatcacgga ctggtggggc aacggcatca cggaccgcta
     1261 ttacggcaac gacagcggca ccaagaaggt ggacgtgttc ctgaaattca ccgacgacca
     1321 ggcctcgggc ctggggatgc ccctgcccgc gggcaagatc cgcgtctacc agaaggacga
     1381 cgacggcaac cgggaattcc tgggcgagga cgcgatcgac cacacgcccc aggacgagca
     1441 ggtgctggtg cgcatgggca cggccttcga cgtggtgggc gaaaggacgc agaccgcctt
     1501 cacccagccc gacgcccatt cgatcgagga atccttcgcc atcacggtga agaaccacaa
     1561 gaagcaggcc gtccaggtgg acgtggtgga gcacctctac cgctattcca actggaagat
     1621 cgtggagaag agccaggact tcaataaagt cgacgcccgg acggtgtcct tcccggtgtc
     1681 cgtccccgcc ggcggcaagg ccacggtgac ctacaaggtg cgttattggt ggaactgagg
     1741 ccgccttccc caagcccctt cccggacggg aaggggcttt tttattggaa agctccgggc
     1801 ataatgaggg ccaagcttga tcccgaccgg agggggccat gttctgccga cgctgcaagg
     1861 tgaagatgcg ggagttggcc cattcggccc ataagaagcg caagttcgtc tgccccaagt
     1921 gcggggccgc ccgcatgatg ggcggcaaat cgaaggccag cgaccgtcgg cggctggagc
     1981 gttactgatc tttcagtcaa agggaatggg gtttcagacg acgacacccc tcaccccgcc
     2041 ctctcccgca aggggagaag gtccttattg tcccggatcc tgaaggaaaa ggaccgggtt
     2101 gtcgggtttt gggatggcct gcagcaacca aggataggtc acttgttggg ccaagaccgc
     2161 gtccgccggg ggtggggcgg tccgccaatg caccaccacc tgaccggctt gctcctccac
     2221 tgcggtcact tccacccgat agcctggcgt cggctccaga cccgcctcca aaaagaccac
     2281 tgcctgggtg gtgaagtcca ctggcgggac gggtccgccg ggcttcaggg atcgccagac
     2341 cttttcaaaa gcggcttcgt cggtgatgag gctttgccca gcggtttggg agagcggatc
     2401 ctgatcgccc gaccaggatg cggtttttcc attccgcatg gcctgggcgg ccggcaggct
     2461 ccggacggct tggtggggct gagcggcggt ctgggcggaa aattcgggcg cgttggcccc
     2521 gccggaggcc tcatccatcg cggcccgggg agcctgcacg gacttcgctt caaaggcttg
     2581 cgggggggcg cctgctgcgg gcggaccggc ggcgggcgcc atggccgggg cgggcgcggc
     2641 ggcgttcgac agggccgccg gtcccggcgc cttcatgatg gaggccgtga ccttcttggc
     2701 cgcgacctct tcggacgggg tttggggtgc cgcgggcggg ttgggcgagc ggacgatctc
     2761 ctcccaccgt ggattctcgg tgaagaaaaa gaccgtggtg gccgcggcgg ccgccagggg
     2821 ccagagggtc cggccccagc cgcccgaagt cctacccagg ccttccttcc gcagcctcgc
     2881 catgatcttg ccgtgcaggt cccaggggag cgggggttcg ggctgccggc gcagaacgtc
     2941 ccggagccgc ctcaaggatt ccaactcggc ccgggctgcg gggcaggcgg acaggtgggc
     3001 cctcactttt ttccgttccc cgggcgtgag ggcgccctcg gcttccaggg gcagcagtgg
     3061 gtggaccttc gcgcagttcc tcataaccaa tccttcagtt gttcccgcaa tagggcccgg
     3121 ccccgggcga tgcgggattt caccgtgccc agccccaacc gcagcatctg ggcgatccgg
     3181 tcatagtcca ggtcctccag gtcccgcagg accacggctt ccttgaaatc cggcggcagc
     3241 ccgctcaggg cggcctccaa ggccttgcgg aattccttgg attccatcag ttcgggcgcc
     3301 tggcgggtct tcttgtccgg cgcccggccc agccagtcgc cctcggcgtc ct
//
LOCUS       DASTJJ010000209         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_275858, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000209 DASTJJ010000000
VERSION     DASTJJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275858"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VFR02_02550"
     CDS             complement(<1..1494)
                     /locus_tag="VFR02_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9869366.1"
                     /translation="MANPGSNQTLESIRVLLVEDDPDDYELIRRYIAQIKNSRYEFTW
                     ASSFEEGLEKLSGPSYHLILLDFRLGARTGLEFLEEADRRHCNAPVVFLTGLDDLETD
                     VKTMRAGAADFVSKQRLDPFLLERTIRYALERSRAARDLKRSEENFRSLIELLPEGLM
                     VHRDDRILYVNPTLVRLLGYSSPDQLIGRSPLDIIHPDYREVAEKRIDMVAQGEGHQY
                     NPPAERILLRQNGDPIYAEAEGLSIIFEGRRAITTILRDITEKKRAEEELRESHFFLE
                     KAQEAAHLGSWISEIDPEGSLLWSKECYRIFGSDPDHPLKAKDFFDRVHPEDLPAVRQ
                     ATERALSENAPYNLDHRILMPNGSIRWVNEQGEVIRDRAGAPLKMVGVVQDITERKIA
                     EQALRRSENQFRAVFDGALEAMLIIDDEGRILDANNSACSLLECGKAELLKRRVDGFF
                     ESDFDFQWARKSFMTTGLFSGKVRLRKSATESLDAEFTARGHILPGRH"
     gene            complement(1504..>1909)
                     /locus_tag="VFR02_02555"
     CDS             complement(1504..>1909)
                     /locus_tag="VFR02_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869367.1"
                     /translation="FQVLEKIRRSNQTRDIPVIIFSVLGEPRDVKKALEMGANDYTVK
                     GFYTPRQVLSKIKNLITHAGSNPRINSYRIRVAPEGPSALALQEDLGLTGGFQCPECQ
                     APLEVEFFPDYARADGHWFASRLVCGRCQKPF"
BASE COUNT          305 a          578 c          658 g          368 t
ORIGIN      
        1 gtgccgcccg ggcaggatgt ggccgcgggc cgtgaattcg gcgtccagcg attcggtggc
       61 gctcttgcgc aggcgcacct tgccgctgaa gagcccggtg gtcatgaagg acttgcgggc
      121 ccattggaag tcgaaatcgg actcgaaaaa gccgtccacc cggcgtttga gcagctccgc
      181 cttgccgcat tccagcaggg aacaggccga gttgttggcg tccaggatcc ggccctcgtc
      241 gtcgatgatc aacatggctt ccagggcgcc gtcaaagacc gcccggaact ggttctcgct
      301 gcgccgcagg gcctgttcgg cgatcttgcg ttcggtgatg tcctgcacca cccccaccat
      361 cttgagcggg gccccggccc ggtcccggat gacctcgccc tgctcgttca cccagcggat
      421 ggagccgttg ggcatgagga tgcggtggtc caggttgtag ggcgcgttct ccgacagggc
      481 ccgctcggtg gcctgccgca cggcgggcag gtcctccggg tggacgcggt cgaagaaatc
      541 cttggccttc agggggtggt ccgggtccga gccgaagatc cggtaacatt ccttcgacca
      601 caacaggctc ccctcggggt cgatctcgga gatccaactt cccaagtggg cggcttcctg
      661 ggccttttcc aggaagaaat ggctttcccg cagctcctcc tcggcccgct tcttctcggt
      721 gatgtcgcgc aggatggtgg tgatggcccg ccgtccctcg aagatgatgg acagcccctc
      781 ggcctcggcg tagatggggt cgccgttctg gcgcagcagg atccgctcgg cgggcgggtt
      841 gtattgatgg ccttcccctt gggccaccat gtcgatgcgc ttctcggcca cctcccggta
      901 gtcgggatgg atgatgtcca ggggggagcg cccgatcaac tggtcggggc tggaatagcc
      961 gagcaggcgg accaaggtgg ggttcacgta gaggatgcgg tcgtcccggt gcaccatgag
     1021 gccttcgggg agcagttcga tgaggctgcg gaagttctcc tcggatcgtt tcaggtcgcg
     1081 ggcggcgcgg gaccgctcca gggcgtaacg gatggtcctt tccaacagga aggggtccag
     1141 ccgctgcttg gacacgaaat cggcggcgcc ggcccgcatg gtctttacgt ccgtctccag
     1201 gtcgtccaaa ccggtcagga acaccacggg cgcgttgcag tgccttcggt cggcctcctc
     1261 caggaattcc aggccggtgc gcgcgcccag acggaaatcc agcaggatca aatgatagga
     1321 agggcccgag agcttctcca atccttcctc gaagctggag gcccaggtga attcgtagcg
     1381 ggaattcttg atctgggcga tgtagcggcg gatcagctcg taatcatccg ggtcgtcctc
     1441 caccaggagg acgcggatgg attccaaggt ttggttgctt ccggggttcg ccatgggccg
     1501 cgcctaaaag ggcttttggc accggccgca gaccagccgg gaagcgaacc aatggccgtc
     1561 ggcgcgggcg taatcgggga aaaactccac ctccaggggt gcctggcatt cggggcattg
     1621 gaaaccgccg gtcaagccca ggtcttcctg gagggccagg gccgagggtc cctcgggggc
     1681 gacccggatc cggtaggaat tgatccgggg gttggagccc gcgtgggtga tcaggttctt
     1741 gatcttgctg aggacctgcc ggggggtgta gaagcccttg accgtgtagt cgttggcgcc
     1801 catttccagg gctttcttca cgtcccgggg ctcgcccagg accgagaaga tgatgaccgg
     1861 gatgtcccgg gtctggttgc tgcgccggat cttttccagc acctggaac
//
LOCUS       DASTJJ010000210         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_153793, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000210 DASTJJ010000000
VERSION     DASTJJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153793"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1145)
                     /gene="mltG"
                     /locus_tag="VFR02_02560"
     CDS             complement(102..1145)
                     /gene="mltG"
                     /locus_tag="VFR02_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9869368.1"
                     /translation="MRRPKGKAARVLAAASIAFLAGLLYVFFPPFFRSHQGVEIPVYS
                     KSTARQIARDLRDKGVLTLAAPFRLLAQLTRADRRLKSGLYQLSPSMSLWQVLDTLSS
                     GKSELLTLRVPEGFTAQQIGGELERMGVVSEADFLKGIQDPALTGRLGVRGPSLEGFL
                     FPQTYRVPLGASVSDLVALMSGEFTQAVGPDFEKHCRARNLTLYQAVILASIVEKEAK
                     TAEDQRVVAGILYNRLRAKMRLEVNATLNYVLDFKDPWLRQDQLGTQSPYNTYLHRGL
                     PPTPICNPGLTALSAVEDPADTPYLYYVAKGDGTNLYAVTFAEHQKNVALAKHLRHLK
                     RVRAALEARNAAP"
     gene            complement(1142..>1299)
                     /gene="ruvX"
                     /locus_tag="VFR02_02565"
     CDS             complement(1142..>1299)
                     /gene="ruvX"
                     /locus_tag="VFR02_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9869369.1"
                     /translation="DERLTSKQAERLMIEGGARRDQRREKVDQVAAALILESALRGAP
                     LAPVEPS"
BASE COUNT          223 a          414 c          447 g          215 t
ORIGIN      
        1 cgcccgaggc gtggaccttc gtcaggtgga acagttggat catcatgggg gctaaattac
       61 ggcaggaacc cgccgaccgc aaggaagaaa ggggcggcgc ctcacggggc ggcgttcctg
      121 gcttccaagg ccgcgcgcac ccgcttgagg tgccgcaggt gcttggccaa ggccacgttc
      181 ttctggtgtt cggcgaaggt cacggcgtaa aggttggtcc catccccctt ggccacgtaa
      241 taaaggtagg gggtatccgc ggggtcctcc acggcgctca aggccgtgag ccccggattg
      301 cagatgggcg tggggggcag gccccggtgc aggtaggtgt tgtagggcga ctgggtcccc
      361 aattggtcct gccgcagcca ggggtccttg aaatccaaca cgtaattcag ggtggcgttg
      421 acctccaggc gcatcttggc gcgcagccgg ttgtagagga tgcccgccac cacgcgctgg
      481 tcctcggcgg tcttggcctc tttttccacg atggaggcca ggatgaccgc ctggtacagg
      541 gtgaggttcc gggcccggca atgcttctcg aaatccggcc ccacggcctg ggtgaattcc
      601 ccggacatca gggcgaccag gtccgacacg ctggcgccca gtggcacccg gtaggtctgg
      661 gggaaaagga agccttccag gctggggccc cgcacgccca gccgacccgt caaggccggg
      721 tcctggatgc ccttgaggaa gtcggcctcc gacaccacgc ccatgcgctc caattcgccg
      781 ccgatctgct gggcggtgaa tccctcaggc acccgcaggg tcaacagctc gcttttaccc
      841 gaggagaggg tgtccaggac ctgccacagg ctcatggaag gcgacagttg gtagaggccg
      901 gacttgagcc gcctgtccgc gcgggtcaac tgggccaaca ggcggaaggg ggcggccagg
      961 gtcagcacgc ccttgtcccg gaggtcccgg gcgatctggc gcgcggtgct cttggaatag
     1021 accgggatct ccacgccctg atgggaccga aaaaaagggg ggaaaaagac gtacaacaaa
     1081 cccgccagga aggcgatcga agccgccgcc agaacccggg cggcttttcc tttgggtctt
     1141 ctcatgacgg ctccaccggc gccaggggcg cgccccgcag ggcggattcc aggatcaggg
     1201 ccgcggccac ctggtcgacc ttttcccggc gctggtcgcg ccgggcgccc ccttcgatca
     1261 tcaaccgttc ggcctgcttg ctggtgaggc gctcatcca
//
LOCUS       DASTJJ010000211         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_31466, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000211 DASTJJ010000000
VERSION     DASTJJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31466"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..698
                     /locus_tag="VFR02_02570"
     CDS             63..698
                     /locus_tag="VFR02_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9869370.1"
                     /translation="MFGTLAPTELAVISSACVEKKVRKGEILFSAGDEPKGLYVIVKG
                     SLSAYRENHEGREQIIHLEQAGTTMAEVPVFDDKPYPSSVRAEEDSELLFLSGENVKK
                     LFTKHPSIALSALRLMASRLRKTAALLESISLREVDQRLASFLLHEFQEKKSKKMKLP
                     SLSILASRLGSVREVVSRALAKLEKDGFIAVDRQRFATLLDEKGLRGFIGR"
     gene            complement(706..1188)
                     /locus_tag="VFR02_02575"
     CDS             complement(706..1188)
                     /locus_tag="VFR02_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9869371.1"
                     /translation="MRREKFLWPLTQGHHGALVAARNIRARLSGAPGEGPGAPESLVR
                     EVEAFFRDDLEFHFKGEEEVLLKAFEEHVGPSDEGSRKIREDHRVLRELSRSSRRTDL
                     SQFAEALTAHIRYEEDVFFPRVEAELTAGEKERLAEELAGFRPGGTHVPPPVEGPQKS
                     "
     gene            complement(1228..>1894)
                     /locus_tag="VFR02_02580"
     CDS             complement(1228..>1894)
                     /locus_tag="VFR02_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9869372.1"
                     /translation="YWTAQPAINLALGAFFSAMEVVPLLLLTLEAYDFAKLRRGEAGT
                     KESDGFFAHYWAIMFLIAVGFWNFFGAGIFGFLINLPIVSYYEHATYLTPNHGHAALM
                     GVYGNLAIAALVFVLRFLVKPEHWSNGILKLSFWSLQIGLMLMLLLDIFPVGVLEMVQ
                     SFTHGYWFARSADFWNQAIVQKLTWARVVGDLTFVVGGVLPLLYFTARSMFFLKKPDA
                     SPE"
BASE COUNT          421 a          558 c          548 g          367 t
ORIGIN      
        1 ttttttaggg gggtcactat gattggggaa tggataagat cgccgcctta aagaagacgg
       61 aaatgttcgg gaccttggca cccaccgaat tggcggtgat ctcctcggct tgcgtcgaaa
      121 agaaagtgcg gaaaggggaa atccttttct ccgccggtga cgagcccaag gggctttatg
      181 tcatcgtgaa ggggtccttg agcgcctaca gggagaacca cgaaggaagg gaacagatca
      241 tccatttgga gcaggcgggg accaccatgg cggaggtccc ggtctttgat gataagccct
      301 atccttcgtc cgtccgggcc gaggaggaca gtgagctcct cttcctctcg ggggagaacg
      361 tcaaaaagct cttcaccaaa cacccctcga tcgccttatc cgcccttcga ttgatggcct
      421 ctaggctccg gaagaccgcc gcccttttgg aatccatctc cttgcgagaa gtcgaccagc
      481 gtttggcttc cttcctgctc catgaatttc aggaaaagaa gtcgaaaaaa atgaaacttc
      541 cctccctctc gatcctagcc tcccggctcg ggtcggtccg ggaggtcgtc tcgcgtgccc
      601 tggccaaatt ggagaaggac ggcttcattg ccgtcgaccg gcaaaggttc gccactttat
      661 tggatgaaaa gggattgcgg ggtttcatcg ggcgctagct gtggcctaag acttctgtgg
      721 accttccacg ggcgggggaa catgggtccc gccgggcctg aacccggcca gctcttcggc
      781 caaacgttcc ttttcaccag ccgtcaattc cgcctccacc ctgggaaaga atacatcttc
      841 ctcgtaccgg atatgcgcgg tcaaagcctc ggcgaactgg gataggtccg ttctccggga
      901 agaacgtgac agctcccgaa gcacccggtg atcctcgcgt atctttcggg aaccctcgtc
      961 cgagggtccg acatgctctt cgaaagcttt gaggagcacc tcttcttcgc ctttgaaatg
     1021 aaactcaaga tcatcccgga agaaggcctc gacctccctc accagggact caggcgcccc
     1081 ggggccctct cctggggccc cggaaagcct ggcccggatg ttccgggcgg ccaccaaagc
     1141 cccatgatgt ccctgggtca ggggccaaag gaacttttcc cgcctcaagg taaggtaccc
     1201 cgtctcaacg cgtctcccct tggaccgtca ttccggggac gcatccggct tcttgaggaa
     1261 gaacatcgaa cgagccgtga aataaagcag gggaaggacc ccgccgacca cgaaggtcag
     1321 gtcccccacc acacgggccc aggtcaactt ctggacgatg gcttggttcc agaaatccgc
     1381 gcttcgggcg aaccaatagc catgggtgaa gctttggacc atctcaagga cgcccacggg
     1441 gaaaatatcc agcaggagca taagcatgag gccgatctgc agcgaccaaa aggatagctt
     1501 caggatcccg ttggaccagt gttccggctt cacgaggaac ctcaggacga agaccagcgc
     1561 cgcgatggcc aggttgccat agacccccat caaggctgcg tggccgtggt tcggcgtcag
     1621 gtaggtcgcg tgttcgtaat agctgacgat gggcaggttg atgaggaacc cgaaaatccc
     1681 ggcgccaaag aagttccaga aaccgacggc gatcaggaac atgatggccc aataatgggc
     1741 gaaaaagccg tccgattcct tggtgcccgc ttccccccgc cgcaatttgg cgaagtcata
     1801 ggcttccagg gtcagtagga gcaaggggac cacttccatc gcggaaaaga aggcccccaa
     1861 cgccaggttg atggcgggtt gggccgtcca gtaa
//
LOCUS       DASTJJ010000212         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_31470, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000212 DASTJJ010000000
VERSION     DASTJJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31470"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFR02_02585"
     CDS             complement(<1..330)
                     /locus_tag="VFR02_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9869373.1"
                     /translation="MDQALVELEKVKVLFDGFKALDVGAFAVEENELRVLIGPNGAGK
                     TTLCDVISGKTRATEGQVRFEGRDITAEPEEEISRMGVGRKFQTPTIFDSLTVFENME
                     LALPGRRK"
     gene            complement(336..1454)
                     /gene="urtC"
                     /locus_tag="VFR02_02590"
     CDS             complement(336..1454)
                     /gene="urtC"
                     /locus_tag="VFR02_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET9869374.1"
                     /translation="MTENLKATLDRALPWSFLFLFLVLVPLFDVAGWMSPETINQWGR
                     FGCFAIAALGLDLLWGYTGLLGLCQSFFFTLGAYAMGMYLAMHGPLDGPGIPRCLYVV
                     SSDVSGFNLPWFWKPFGSFPVAVLLGLLLPGLAAFIIGYSTFRSRVRGVYFSILTQAI
                     TLGGWLVFCRNSIRLCGTNGLTNFVTLAGFDLSKGSVQLGLYMATVVVLALAYVGCRW
                     LVGSRLGRVLLAIRDKESRLRFSGYRPTSYKVFIFVVAAMLAGLGGMLYTPQMGIMTP
                     THMTVESSILMVVWVAVGGRGTLSGAILGALLVNSLYSFLTTRWPHSWLFVEGGLFIS
                     VVLFFPEGLMSLWRKWMVRKEVDLSVPVELAPAAEPKE"
     gene            complement(1447..>2623)
                     /gene="urtB"
                     /locus_tag="VFR02_02595"
     CDS             complement(1447..>2623)
                     /gene="urtB"
                     /locus_tag="VFR02_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET9869375.1"
                     /translation="DWGMMAHLTARQAVETDPKVKAALAENADLLAVKDPAQAGPQAL
                     LSAVKALGRDHSLRSLSSLQALVPAAQDPALRKALASSIAAIDHWQWLVNVVQDAFSG
                     LSLGSILVLMALGLSIIFGLMGVINMAHGELMTMGAYTGFLMQEAFHRWAPGAQDWYL
                     PCAIPLAFLTAGAVGYLMEALIIRHLYGRPLETLLATWGVSLCLIQAVRLVFGDNQAV
                     NSPTWLVGGLELFHGVVLPYNRCFIILLCGLSVAFTYAVIGRTKFGLLIRATTQNRTM
                     ASSLGVNMRRVDALTFAFGAGLAGVAGCALTQVGGITPDMGSNYIVDSFLVVVTGGVG
                     KLAGAVWAGFGLGCLNKFLEPFVQAVWGKVLILLFVVVFIQWRPSGLFPAKGRAAND"
BASE COUNT          504 a          887 c          883 g          349 t
ORIGIN      
        1 cttgcgcctt ccgggcaggg ccagctccat gttctcgaac accgtcaggg agtcgaagat
       61 ggtgggggtc tggaacttgc ggcccacgcc catgcggctg atctcctcct cgggctcggc
      121 cgtgatgtcg cgcccctcga agcgcacctg cccctcggtg gcccgggtct tgccgctgat
      181 gacgtcgcac agcgtggtct tgcccgcgcc gttggggccg atcaagaccc gcagttcgtt
      241 ctcctccacg gcgaaggccc ccacgtccag ggccttgaag ccgtcgaaaa gcaccttcac
      301 cttttccagt tccaccaggg cttggtccat gggcgtcact ccttgggctc ggccgcgggg
      361 gccagttcca ccggcacgga caggtccact tccttgcgga ccatccactt gcgccacagg
      421 ctcatgagcc cctcggggaa gaagagcacc acgctgatga agaggccgcc ttccacgaag
      481 agccaggaat ggggccagcg ggtggtgagg aagctgtaga gcgagttcac caaaagggcc
      541 cccaggatgg cccccgacag cgtgccccgg ccgcccacgg ccacccagac caccatgagg
      601 atggagcttt ccaccgtcat gtgggtgggc gtcatgatgc ccatctgggg cgtgtagagc
      661 atgccgccca accccgccag catggcggcc accacgaaga tgaagacctt ataggaagtg
      721 ggccggtagc ccgagaaccg caggcgcgat tccttgtccc ggatggccaa gagcacccgc
      781 cccagccggg accccaccag ccaccggcac cccacgtagg ccagggccag caccaccacg
      841 gtggccatgt aaaggcccag ttggaccgaa cccttgctga ggtcgaaccc ggccagggtc
      901 acgaaattgg tgaggccgtt ggtgccgcac aaccggatgc tgttgcggca gaagaccagc
      961 cagcctccca gggtgatggc ctgggtcagg atggaaaagt agacgccccg gacccggctg
     1021 cggaaggtgg aatagccgat gatgaaggcc gccaggcccg gcagcagcag ccccaacagc
     1081 accgccaccg ggaaggagcc gaagggcttc cagaaccagg gcaggttgaa gcccgagacg
     1141 tccgaggaca ccacgtacag gcagcggggg atgcccgggc cgtccagggg cccgtgcatg
     1201 gccaggtaca tgcccatggc gtaggcgccc agggtgaaga agaaggactg gcagagcccc
     1261 aaaagccccg tatagcccca taaaaggtcg agccccaggg ccgcgatggc gaagcagccg
     1321 aagcgccccc attggttgat ggtctcgggg gacatccacc ccgccacgtc gaagagcggc
     1381 accagcacca ggaagaggaa caggaaggac cagggcaggg cccggtccag cgtcgccttg
     1441 aggttttcag tcattggccg ccctcccctt ggcggggaag aggcccgacg gccgccactg
     1501 gatgaagacc accacgaaga gcaggatcag caccttgccc cagacggcct gcacgaaggg
     1561 ctccaggaac ttgttcaggc agcccaggcc gaaccccgcc cagacggcgc cggccagctt
     1621 gcccacgccg cccgtcacca ccaccaggaa cgagtccacg atgtagttgg agcccatgtc
     1681 gggggtgatg ccccccacct gggtgagcgc gcagcccgcc accccggcca ggcccgcccc
     1741 gaaggcgaag gtgagcgcgt ccacccggcg catgttcacg cccaagctgc tggccatggt
     1801 gcggttctgg gtggtggccc ggatgagcag gccgaacttg gtgcggccga tgaccgcgta
     1861 ggtgaaggcc acgctcaggc cgcacaacag gatgatgaag cagcggttgt agggcagcac
     1921 cacgccgtgg aaaagctcca gcccgcccac cagccaggtg gggctgttga cggcctggtt
     1981 gtccccgaac accaggcgga ccgcctggat caggcaaagg ctcacgcccc aggtggccag
     2041 gagcgtctcc agggggcggc cgtaaaggtg gcggatgatg agcgcttcca tcaggtaccc
     2101 caccgccccc gcggtgagga aggccagcgg gatggcgcag ggcaggtacc aatcctgcgc
     2161 gcccggcgcc cagcggtgga aggcctcctg catgaggaag cccgtgtagg cgcccatggt
     2221 catcagttcc ccgtgcgcca tgttgatgac gcccatcagg ccgaagatga tggaaaggcc
     2281 cagggccatc agcaccagga tggaacccag ggaaaggccg ctgaaggcgt cctgcaccac
     2341 gttcaccagc cactgccaat ggtcgatggc cgcgatggag gaggccaggg cctttcgcag
     2401 cgccgggtcc tgggcggcgg ggacgagggc ctgcaggctg gaaagcgacc gcagcgaatg
     2461 gtcccggccc agggccttca cggccgacaa gagcgcctgg ggcccggcct gggcggggtc
     2521 cttcaccgcc aagaggtccg cgttctccgc caaggcggcc ttcaccttgg ggtcggtctc
     2581 cacggcctgg cgggccgtca ggtgggccat catgccccag tcg
//
LOCUS       DASTJJ010000213         1749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_31681, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000213 DASTJJ010000000
VERSION     DASTJJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31681"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="plsX"
                     /locus_tag="VFR02_02600"
     CDS             <1..978
                     /gene="plsX"
                     /locus_tag="VFR02_02600"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET9869376.1"
                     /translation="VLRMADQDLEIVLVGRQEDIERELADLGGYPKKSISIVNAREVV
                     EMGESPAQACKQKRDSSIVRCSELVKRREVAACASAGNSGATMAAALMTCGRIDGVLR
                     PAITTMVPSVQGVSVLVDAGANVDCKAKHLLQFALMGDVFARTVLGFKNPRVGLLSIG
                     EEETKGNELVFETQKLLKNAPLNYIGNVEGRDIVNGNCDVTVCDGFVGNVTLKAIEGV
                     GELVYNLLKKEMQSNLFSLVGALLASSAFRRFKRSIDYKEYGGAPLLGIDGISMISHG
                     KSNAFAMKNAIRATLRCNNQHMNEILTERIQKYGQVNDKPETKPTQESK"
     gene            975..>1749
                     /locus_tag="VFR02_02605"
     CDS             975..>1749
                     /locus_tag="VFR02_02605"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9869377.1"
                     /translation="MNGKTRAGFLGTGSYLPEEVLTNQDMEKIVETTNDWIVARTGIK
                     ERHRAAPTQATSDLAVEAAKKALDAAGLKPTDIQLIIVATITADMPTPSTACLVQSKL
                     GAPQAAAFDVSAACSGFVYGLAIAKAFVESGMYRNILLIGAEKLTAFVDWKDRGSCVI
                     FGDGAGAAVIGPVAEGQHEILSSFMVANGQEADLLKIPGGGSLTPASPDSLTQGLHYL
                     KMQGKETFKVAVKVMADAILEALRLAGLTLDQVSLIIPHQ"
BASE COUNT          348 a          579 c          559 g          263 t
ORIGIN      
        1 gtcctacgca tggccgacca ggacctggag atcgtcctgg tggggcgcca agaggacatc
       61 gagcgcgagc tggccgacct gggcggttac cccaaaaagt ccatctccat cgtgaacgcc
      121 cgggaagtgg tcgaaatggg cgaaagcccg gcccaggcct gcaagcagaa acgcgactcc
      181 tccatcgtgc gttgctccga attggtgaaa cgcagggaag tggcggcctg cgcctccgcc
      241 ggcaactcgg gcgccaccat ggcggcggcc ctcatgacct gcggccgcat cgacggggtg
      301 ctgcgccccg ccatcaccac catggtcccc agcgtgcagg gcgtctccgt gctggtggac
      361 gcgggcgcca acgtcgactg caaggccaaa cacctgctcc agttcgccct gatgggcgac
      421 gtcttcgccc gcacggtgct gggtttcaag aacccccggg tgggcctgct ttccatcggg
      481 gaagaggaga ccaagggcaa tgagctggtc ttcgagaccc aaaagctcct gaagaacgcc
      541 cccttgaact atatcggcaa cgtggaaggc cgggacatcg tcaacggcaa ctgcgacgtg
      601 acggtctgcg acggtttcgt gggcaacgtg acgctcaagg ccatcgaggg cgtgggtgag
      661 ctggtgtaca accttttaaa gaaggaaatg cagtccaacc ttttctcgct ggtgggggcc
      721 ctgctggcct cctcggcctt ccgccgcttc aaacgcagca tcgactacaa ggaatacggg
      781 ggcgcgccgc tgttgggcat cgacggcatc agcatgatca gccacggcaa gtccaacgcc
      841 ttcgccatga agaacgccat ccgggccacc ctgcgctgca acaaccagca catgaacgag
      901 atcctcaccg agcggatcca gaaatacggc caggtcaacg acaagccgga aaccaagccg
      961 acccaggagt cgaagtgaac ggaaaaacga gggcggggtt cctgggcacg ggttcctacc
     1021 tgcccgagga agtgctgacc aaccaggaca tggaaaagat cgtggagacc accaacgatt
     1081 ggatcgtggc ccggaccggc atcaaggaac gccaccgggc ggccccgacc caggccacct
     1141 cggacctggc ggtggaggcg gccaagaagg ccctggacgc cgcgggactg aagcccaccg
     1201 acatccagct catcatcgtg gccaccatca cggccgacat gcccacccct tccacggcct
     1261 gcctggtgca gagcaagttg ggggcccccc aggccgcggc cttcgacgtg tcggcggcct
     1321 gttcgggttt cgtctacggc ctggccatcg ccaaggcctt cgtggaaagc ggcatgtacc
     1381 gcaacatcct gctcatcggg gccgagaagc tgaccgcctt cgtggactgg aaggaccggg
     1441 ggtcctgcgt cattttcggc gacggcgccg gggccgcggt catcgggccg gtggccgagg
     1501 ggcaacacga gatcctgtcc tccttcatgg tggccaacgg ccaggaggcc gacctgctca
     1561 agatccccgg cgggggcagc ctgacccccg cctcgcccga ttcgctgacc caagggctcc
     1621 attacctcaa gatgcagggc aaggagacct tcaaggtggc cgtgaaggtc atggccgacg
     1681 ccatcctgga ggccctgcgc ctggcggggc tgaccctgga ccaggtctcg ctcatcatcc
     1741 cgcaccagg
//
LOCUS       DASTJJ010000214         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_31864, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000214 DASTJJ010000000
VERSION     DASTJJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31864"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /gene="mfd"
                     /locus_tag="VFR02_02610"
     CDS             <1..1996
                     /gene="mfd"
                     /locus_tag="VFR02_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9869378.1"
                     /translation="DWAVHVDCGICLYRGLTPLTIDGVTREYIQLEFADSEKIYLPTD
                     QIALVQRYIGAEGAPVLSKLGNEQWSRTKAKVRREIEAVARELMALYSTRQALKKPPA
                     PPDTPWQHEFEDAFLYELTPGQYAAVRDVKKDMESDRPMDRLVCGDVGYGKTEVAMRA
                     AFKAVQDKKQVAVLVPTTILAQQHYLTFKERMAEYPVDIQMLSRFRSPAEIKKTVEAA
                     REGRVDVVIGTHRLLGKDVKFARLGLLIIDEEHRFGVSQKEKLKALKADVDVLTLTAT
                     PIPRTLHMSLSGIREISIIDTPPQNRLSVSSHVGPMDERLVAEAIRREMNREGQVFYV
                     HNHVRDIERIADQLHRLVPEAKLAVAHGQLTEQELESIMMDFIDREYHVLVCTSIIES
                     GLDMPNVNTLIVERTDLFGLSQLHQLKGRVGRTNRQAYAYFFYPRHMTLRELAQKRLE
                     VLQEFSSLGSGMHIAMKDMEIRGAGNVLGTQQHGTLDSIGFDLYSRMLSQELAQRKGE
                     EAPLDFTPSLSLGLSAYLPKDYIPEETVKIEFYRRLVEAKDAEALARIADELKDRFGE
                     PPPEAQALLKVAALRPAVKALGIQKLEAQGGWVFLQWHPDLAPEGVRVEQWMRRWPPT
                     RIRFSPNDAHSISFRVAKGEEPPEARLASVQKLLQDLATA"
     gene            2182..2925
                     /locus_tag="VFR02_02615"
     CDS             2182..2925
                     /locus_tag="VFR02_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869379.1"
                     /translation="MSSRWKAFFLILAGGLTALLGLACNEYGAAGVKGSDVVATVGDT
                     KVTFADWMRQMDLLRVFSPQPIDPDNADQARQVLQSLEDQDVVLAALRKAKYSDPKFD
                     EAMQKELIQADLQLKQIKDKLKRDQETVDRLQNNYKDAYTKMLLSQHFAASQVDSVYV
                     SDKEVRERYDQMAQAARKQGQQFPPYAKVKDRVKLRLQADKLLTQFQGDTKIEQKDAV
                     IQKYLASLSPSEQALDANPSAAAAPAGKP"
     gene            2944..>3908
                     /locus_tag="VFR02_02620"
     CDS             2944..>3908
                     /locus_tag="VFR02_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9869380.1"
                     /translation="MASPRRALVPSLARRGLALGLLLALPACEPTLQFKAPQVPPDKT
                     AVLLNGEPVSLEEFDTEFRLMAIHYSAVSEGQMRAIKRRLFDQVVDRRLLVQEARKEG
                     VSLTRREWNRDVQQAFREMPDDFLEILKMQGVSEEAWKRKLLQEKLAAKLVDKDVNSL
                     VQITPQEVEDYYWSHLGDYWKPESVHTLHLVVKKHGDLEKALKRLKAGEDFSKVAADL
                     SLADDKSRGGDWGDLPVDRVPAPYLALLRGLKPGEISRPLHDNFGYHLFQLVGFTPRK
                     MQDFAQVRKQIDDSLLKEEQDLRFDQWMADLKRRAVIRINPDLAPV"
BASE COUNT          739 a         1322 c         1259 g          588 t
ORIGIN      
        1 cgactgggcg gtgcacgtgg actgcggcat ctgcctgtac cggggcctca ccccgctcac
       61 catcgacggc gtgacccgcg aatacatcca gttggagttc gccgactcgg agaagatcta
      121 cctgcccacc gaccagatcg ccctggtgca gcgctacatc ggcgccgagg gcgcgcccgt
      181 gctgtccaag ctgggcaacg agcaatggag ccgcaccaag gccaaggtgc ggcgggagat
      241 cgaggccgtg gcccgggaac tgatggccct ctattcgacc cggcaggccc tgaagaaacc
      301 gcccgccccg cccgacacgc cctggcagca cgagttcgag gacgccttcc tctacgagct
      361 cacgcccggc cagtacgccg ccgtgcgcga cgtgaagaag gacatggaaa gcgaccggcc
      421 catggaccgg ctggtctgcg gcgacgtggg ctacggcaag accgaggtgg ccatgcgggc
      481 cgccttcaag gccgtgcagg acaagaaaca ggtggccgtg ctggtgccca ccaccatcct
      541 ggcccagcag cactacctca ccttcaagga acgcatggcc gagtatccgg tggacatcca
      601 aatgctgtcg cgcttccgga gcccggcgga gatcaagaag accgtggagg ccgcccggga
      661 aggccgggtg gacgtggtca tcggcaccca ccgcctgctg ggcaaggacg tcaagttcgc
      721 ccggctgggc ctcttgatca tcgacgagga gcaccgcttc ggcgtgtccc agaaggaaaa
      781 gctcaaggcc ctcaaggccg acgtggacgt gctcacgctc accgccacgc ccatcccccg
      841 caccctgcac atgtcgctgt ccggcatccg ggagatctcc atcatcgaca cgccgcccca
      901 gaaccgcctg tcggtgtcca gccacgtggg gcccatggac gagcggctgg tggccgaggc
      961 catccggcgg gaaatgaacc gcgagggcca ggtcttctac gtgcacaacc acgtgcggga
     1021 catcgagcgc atcgccgacc agctgcaccg cctggtgccc gaggccaagc tggccgtggc
     1081 ccacggccag ctcaccgagc aggagctgga atccatcatg atggacttca tcgaccggga
     1141 ataccacgtg ctggtctgca cctccatcat cgagtcgggc ctggacatgc ccaacgtgaa
     1201 cacgctgatc gtggagcgca cggacctctt cggcctctcc cagctgcacc agttgaaggg
     1261 ccgggtgggc cgcaccaacc ggcaggccta cgcctacttc ttctaccccc ggcacatgac
     1321 cctgcgggag ctggcccaaa agcgcctgga ggtgctccag gagttctcgt cgctgggctc
     1381 gggcatgcac atcgccatga aggacatgga gatccgcggg gcgggcaacg tgctgggcac
     1441 ccaacagcac ggcacgctgg actccatcgg cttcgacctc tacagccgca tgctgtccca
     1501 ggaactggcc cagcgcaagg gcgaagaggc gccgttggac ttcacgccct cgctgtccct
     1561 ggggttgagc gcctacctgc ccaaggacta catccccgag gagaccgtca agatcgagtt
     1621 ctaccggcgc ctggtggagg ccaaggacgc ggaggccctg gcccggatcg cggacgaatt
     1681 gaaggaccgc ttcggcgagc ccccgccgga ggcccaggcc ctgctgaagg tggcggccct
     1741 gcgtcccgcg gtgaaggcgt tgggcatcca gaaattggag gcccagggcg gctgggtctt
     1801 cctgcagtgg catcccgact tggcgcccga gggcgtgcgc gtcgagcaat ggatgcggcg
     1861 ctggccgccc acccgcatcc gcttctcgcc caacgacgcg cactccattt ccttccgggt
     1921 ggccaagggg gaagaacccc cggaggcccg gctggcctcg gtccaaaaat tgctccagga
     1981 cctggccacg gcctgagggc gggccgatcc gtcctgaagt tcccggcctt cggtcccgcc
     2041 gccgattccg cgcggctccg gggcgctttc cggcctttcg ggggaaagcg cccttgcggg
     2101 ccctaagggc ccatgctagg ataccgcctc ttgtcgaccc cacttcaacc gacggaattc
     2161 aaggaaagaa agggagcttt tatgtcatct cgctggaagg cgtttttcct catcctggcc
     2221 gggggcctga ccgccctcct gggcctggcc tgcaacgaat acggcgccgc gggcgtcaag
     2281 ggcagcgacg tggtggccac cgtgggcgac accaaggtga ccttcgccga ttggatgcgc
     2341 cagatggacc tgctgcgggt cttctccccc cagcccatcg acccggacaa cgccgaccag
     2401 gcgcggcaag tgctgcaaag cctggaggac caggacgtgg tgctggcggc cctgcgcaag
     2461 gccaaataca gcgaccccaa gttcgatgag gccatgcaga aggagctgat ccaggccgac
     2521 ctgcagctca agcagatcaa ggacaagctg aagcgcgacc aggagacggt cgaccggctc
     2581 cagaacaact ataaggacgc ctacaccaag atgctcctgt cccaacattt cgccgccagc
     2641 caggtggaca gcgtgtacgt gagcgacaag gaagtccgcg agcgctacga ccagatggcc
     2701 caggccgccc ggaaacaggg acagcaattc ccgccctacg cgaaggtgaa ggaccgcgtc
     2761 aagctccgcc tgcaggccga caagctgttg acccagttcc agggcgacac caagatcgag
     2821 cagaaggacg ccgtgatcca gaaatacctg gcctccctct cgcccagcga gcaggccctg
     2881 gacgccaacc cgtccgcggc cgcggctccc gccgggaaac cctgaggatc cgggccggcg
     2941 cccttggctt ccccccgtcg ggccctcgtc ccgtccctgg cgcgccgggg gctggccttg
     3001 ggcctgctcc tggcccttcc ggcctgtgag cccaccctcc aattcaaggc cccccaggtg
     3061 ccgcccgaca agacggccgt gctcctgaac ggggagcccg tgtccctgga ggagttcgac
     3121 accgagttcc ggctcatggc catccattac agcgcggtgt ccgaggggca gatgcgggcc
     3181 atcaaacgcc gtcttttcga ccaagtggtg gaccgccggt tgctggtgca ggaagcccgg
     3241 aaggaaggcg tgtccttgac ccgccgcgag tggaaccggg acgtgcagca ggccttccgg
     3301 gagatgcccg acgatttcct cgagatcctg aagatgcagg gcgtcagcga ggaagcctgg
     3361 aaacgcaagc tgctccagga aaagctggcc gccaaactgg tcgacaagga cgtgaattcg
     3421 ctggtgcaga tcacccccca ggaggtggag gactattact ggtcccacct gggggactac
     3481 tggaagcccg agtcggtgca taccctgcac ctggtcgtga agaaacacgg cgacctagaa
     3541 aaggcgctca aacgtctgaa ggcgggggag gatttttcca aggtggcggc cgacctgtcc
     3601 ctggccgacg acaagtcgcg gggcggggat tggggcgacc tgccggtgga ccgggtcccc
     3661 gcgccctacc tggcgctgct gcggggcttg aagcccgggg agatttccag accgctgcac
     3721 gataattttg gatatcatct cttccaattg gtcggattca ccccccggaa aatgcaggac
     3781 ttcgcccagg tccggaagca gatcgatgat tcgcttttga aggaagagca ggacttgcgg
     3841 ttcgaccaat ggatggccga cctcaagcgc cgggccgtga tccggatcaa cccggacctg
     3901 gcgcccgt
//
LOCUS       DASTJJ010000215         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1132947, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000215 DASTJJ010000000
VERSION     DASTJJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132947"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFR02_02625"
     CDS             complement(<1..212)
                     /locus_tag="VFR02_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869381.1"
                     /translation="MKPRPTSIRSRAKTWDQAQWESWARLMAKVQEGDTDAYGRLLDE
                     LGPVLLGYVRKRVFNPELVPDVYQEVL"
     gene            complement(324..1019)
                     /locus_tag="VFR02_02630"
     CDS             complement(324..1019)
                     /locus_tag="VFR02_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET9869382.1"
                     /translation="MADAPKRQYVSFAFYKVSPAFRQLPAEKREEAKKSFTEAVEAFG
                     RDGILLAYSTLGFKADCDLMLWRISYDLDSFQRLESRILKSALGPYLSQPASYLAMTK
                     RSLYIDKHQHEGQDGNRMAIVPGRGKYIFVYPFVKTREWYLLSKEERQRVMDEHIRVG
                     NLYPSVKLNTTYSFGLDDQDFVVAFEGDQPQDFLDLVMALRETEGSKYTVRDTPIYTC
                     RLMEVRETVDLLG"
     gene            complement(1120..>1727)
                     /locus_tag="VFR02_02635"
     CDS             complement(1120..>1727)
                     /locus_tag="VFR02_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869383.1"
                     /translation="LGARAASGKALASWGGLRLLFLRPEPATVMAGSRLQLNYGWERL
                     HASPADGAEWVAVYFTDPAGDYPLKDGLLWFHDIHAPFGGGEAFGALAPGRAFGETRF
                     LFVPSDLPPGRYRLWVALQRPGQAPRLGREAFAGDFYDRAAAEDLEKFTGRPGAGSFT
                     QYAPEDSRSAPNDFLPLASPGGKDGVFAPAGQIEVTAPGAP"
BASE COUNT          289 a          554 c          607 g          277 t
ORIGIN      
        1 agcacctctt ggtacacgtc gggcaccagc tcgggattga agacgcgttt gcgcacatag
       61 cccaagagca cgggccccag ttcgtccaac aggcgcccat aggcgtccgt gtcgccctct
      121 tggaccttcg ccatgagccg ggcccaggat tcccattggg cctggtccca ggtcttggcc
      181 ctggagcgga tggaggtcgg ccttggtttc aatcggccct tcttggaatc tacgggaatg
      241 gaacggcgga agttacccgg cgggcattat atcgggggac cggccccgcc ggcaaaaagc
      301 ttcgcggtcc cccggcgacg gcctcacccc aagaggtcga ccgtctcccg gacctccatc
      361 aaccggcagg tgtagatggg ggtgtcccgg accgtgtact tggagccctc ggtctcccgc
      421 agcgccatga ccaggtccag gaaatcctgg ggctggtcgc cctcgaaggc caccacgaag
      481 tcctggtcgt ccaggccgaa ggaataggtg gtgttgagct tcaccgaagg ataaaggttg
      541 cccacccgga tgtgctcgtc catgacccgt tggcgctctt ccttggaaag caggtaccac
      601 tcccgggtct tcacgaaggg atagacgaag atgtacttgc cccggcccgg cacgatggcc
      661 atgcggttgc cgtcctgtcc ctcgtgctgg tgcttgtcga tgtagaggct gcgcttggtc
      721 atggccaggt aggaggccgg ctggctcagg taggggccca gggcgctctt gaggatgcgc
      781 gattcgagcc gttggaagct gtccaggtcg tagctgatgc gccagagcat gaggtcgcag
      841 tcggccttga accccagggt ggaataggcc agcaggatgc cgtcccggcc gaaggcctcg
      901 acggcctcgg tgaaggactt cttggcctcc tccctttttt ccgccggcag ctgccggaag
      961 gcgggggaga ctttatagaa ggcgaaactc acgtattgtc gcttgggggc gtcggccatg
     1021 tcgggtcctt cccgggatcg gagtggcgtc cggacagcgc ccggacggag gccgtatttt
     1081 aggccgggcc gggccaaaat gcaaaaaagc cgggcggatt cagggggccc cgggcgccgt
     1141 gacctcgatc tggcccgcgg gggcgaagac cccgtccttg ccgccggggg aagccagcgg
     1201 caggaaatcg ttgggcgccg agcgggaatc ctcgggggcg tactgggtga aggatccggc
     1261 gcccggccgg cccgtgaatt tttccaggtc ctcggcggcc gcccggtcgt aaaaatcacc
     1321 ggcgaaggcc tcccggccca gccggggggc ctgcccgggc cgctgcaggg ccacccacag
     1381 gcggtagcgg cccggcggaa ggtcggaggg aacgaagagg aaacgcgtct cgccgaaggc
     1441 ccgccccggg gccagggcgc cgaaggcctc ccccccgccg aagggggcgt ggatgtcgtg
     1501 gaaccagagc aggccgtcct tcagcggata atcacccgcc ggatcggtga aatagaccgc
     1561 cacccattcg gcgccgtccg cgggggaagc gtggagcctt tcccagccgt aattgagctg
     1621 gagcctgctg ccggccatga cggtcgcggg ctcggggcgc aggaagagca gccgcagccc
     1681 gccccaggag gccagggcct tccccgacgc cgcccgcgcg cccagcg
//
LOCUS       DASTJJ010000216         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_399149, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000216 DASTJJ010000000
VERSION     DASTJJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399149"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1047)
                     /locus_tag="VFR02_02640"
     CDS             complement(<1..>1047)
                     /locus_tag="VFR02_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9869384.1"
                     /translation="VAASVGPAVVNISSEWTEDIQGFGNMNDFFNYWFYGPNGGSRAR
                     PEFKQKRRSLGSGFIVSPDGTIVTNAHVVGKAEKITVTFEDGTTYPAKILGKDSNTDI
                     AVIKIIGGKGDFPTVSLGDSDSIKVGEWAIAIGNPFALDHTVTTGVISAKGRSVEIGG
                     EDGSPRGGAQSYIQTDASINPGNSGGPLCDIEGQVIGINSAIYSQSGGSIGIGFAIPI
                     NLAKKVADDLVNKGKVIRAGLGAVVQSLTPPMAKSFGLSSTQGALISSVNPGSAAEKA
                     GLKPGDIVLSLNDELVDSSNDLVTKLYAFAPGDVVHLSVLRDGSQVRLKVVLQTLKDT
                     GLPGQEDQAQQSAGP"
BASE COUNT          157 a          328 c          362 g          200 t
ORIGIN      
        1 ggacccgcgc tttgctgggc ctggtcttcc tggccgggca ggcccgtgtc cttcagggtc
       61 tgcaggacga ccttgagccg cacctgggag ccgtcgcgca gcaccgagag gtgcaccacg
      121 tcgccggggg cgaaggcgta gagcttggtc accaggtcgt tggaactgtc caccagctcg
      181 tcattgaggg aaaggacgat gtcccccggc ttcaagccgg ccttttcggc cgcgctgccc
      241 gggttcacgc tggaaatgag ggccccctgg gtgctggaca ggccgaagct tttggccatc
      301 gggggggtga gggattgcac caccgcgccc aaccccgcgc ggatgacctt gcccttattg
      361 accaggtcgt cggccacctt cttggccagg ttgatgggga tggcgaagcc gatgccgatg
      421 gaaccgcccg attggctgta gatggcgctg ttgatgccga tgacctggcc ctcgatgtcg
      481 caaaggggcc cgcccgagtt gcccgggttg atggaggcgt cggtctggat gtagctttgg
      541 gcgccgcccc ggggggaacc gtcctccccg ccgatctcca cgctgcggcc cttggccgaa
      601 atgacgccgg tggtcaccgt atggtccagg gcgaagggat tgccgatggc gatggcccat
      661 tcgcccacct tgatgctgtc cgaatccccc agggagaccg tggggaaatc gcccttgccc
      721 ccgatgatct tgatgaccgc gatgtccgtg ttggagtcct tgcccaggat cttggccgga
      781 taagtggtgc cgtcctcgaa ggtgaccgtg atcttctcgg ccttgcccac cacgtgggcg
      841 ttggtcacga tggtgccgtc gggggacacg atgaagcccg accccaggct gcggcgcttc
      901 tgcttgaact cgggccgggc gcgggacccg ccgttggggc cgtagaacca gtagttgaag
      961 aagtcgttca tgttgccgaa gccctggatg tcctcggtcc actcggaact gatgttgacc
     1021 accgcggggc ccacgctggc cgcgacg
//
LOCUS       DASTJJ010000217         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1621884, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000217 DASTJJ010000000
VERSION     DASTJJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621884"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFR02_02645"
     CDS             <1..652
                     /locus_tag="VFR02_02645"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET9869385.1"
                     /translation="LSVLGGYATLQVGGPVTVAGRACLSLTALANSAFPFSAVYPVED
                     VQTSYFDAVDFLSWKFENQVHEGGYRAHNLEQYDQLAHRLVRRHNDDQPQTITLPPFA
                     QDIISCFYYFRLLDLQPGRRYTIPTCSSGKNYQLMIDVLGREKVTVPAGTFECLKARP
                     FVKYDTVFRNKEDIVLWVTDDARHLPVKVESAIVIGTISIDLLDATLPEINGKPGP"
     gene            774..1343
                     /locus_tag="VFR02_02650"
     CDS             774..1343
                     /locus_tag="VFR02_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011372206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET9869386.1"
                     /translation="MIDKIRGRIEESIQAKQNLLAQCLPQIEKAGKALVDCYLKGHKA
                     VFFGNGGSAADAQHMAAELVGDYRKRRRAVLSLALHTNTSTLTALGNDYGYDQVYSHQ
                     IEGLCVPGDVAVGISTSGNSQNVLKGILAAKQKGVATVALLGKDGGKIAKEADISIIV
                     PAQDTDRIQESHMLIGHIFCELVESALFP"
     gene            1353..>1814
                     /locus_tag="VFR02_02655"
     CDS             1353..>1814
                     /locus_tag="VFR02_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9869387.1"
                     /translation="MKKNRPKSRNQMVAVDPAELIALVGKLQDKKVVVIGDVMLDVYV
                     KGTVARISPEAPIPVVEINEKDRKMPGGAANVACNITALGGASTIIGVMGRDLAGREL
                     VQELKRRKVDTSGMLALADRPTTEKTRVLAHTQQVVRIDREMKAPLDDRQQD"
BASE COUNT          350 a          629 c          555 g          280 t
ORIGIN      
        1 cctgtcggtc ctgggcggct acgccaccct gcaggtgggc ggcccggtca cggtggcggg
       61 gagggcctgc ctgtccctga ccgcgctggc caactcggcc ttccccttca gcgcggtcta
      121 tccggtggag gacgtgcaga ccagctattt cgacgcggtg gatttcctca gttggaagtt
      181 cgagaaccag gtgcatgagg ggggctaccg ggcccacaac ctggaacaat acgaccaact
      241 ggcccaccgc ctggtgcgcc gccacaacga cgaccagccc cagaccatca ccctgcctcc
      301 cttcgcccag gacatcattt cctgcttcta ttatttccgg ctgctcgacc ttcagcccgg
      361 ccggcgctac accatcccca cctgttccag cggcaagaac taccagttga tgatcgacgt
      421 gctgggccgg gaaaaggtca ccgtgccggc gggcaccttc gagtgtctca aggccaggcc
      481 cttcgtgaag tacgacacgg tcttccgcaa caaggaggac atcgtgttgt gggtgaccga
      541 cgacgcccgc cacctgccgg tcaaggtcga gagcgccatc gtcatcggca ccatcagcat
      601 cgacctcttg gacgccaccc tgcccgaaat caacgggaaa cccggcccct aggctttccc
      661 cttggaaagg gggaggctct ttccgtatga tggcggggct tgccgggcgt ggatatatcg
      721 tcgcggcggc ggtaagatat cccccgttcc catccgtcgt tgaaaggcga ggcatgatcg
      781 acaagatacg aggccgcatc gaggagagca tccaggccaa gcagaacctg ctggcccaat
      841 gcctgcccca gatcgagaag gccgggaagg ccctggtgga ctgctatttg aagggccaca
      901 aggccgtctt cttcggcaac ggcggcagcg ccgccgacgc ccagcacatg gccgccgaac
      961 tggtggggga ttaccgcaag cgccgccggg ccgtgctgtc cctggccctg cacaccaaca
     1021 cctccaccct gaccgccctg ggcaacgact acggctacga ccaggtctac tcgcaccaga
     1081 tcgaggggct ctgcgtcccc ggcgacgtgg cggtgggcat ctccacctcg ggcaactcgc
     1141 agaacgtcct caagggcatc ctggcggcca agcaaaaggg cgtggccacc gtggccctgc
     1201 tgggcaagga tggcggcaag atcgccaagg aagccgacat ctccatcatc gtcccggccc
     1261 aggacaccga ccgcatccag gaatcccaca tgctcatcgg ccatatcttc tgcgaattgg
     1321 tcgagtcggc gttgttccct taaggccccc ccgtgaaaaa gaaccgcccc aagagccgca
     1381 accagatggt cgcggtcgat cccgccgagc tcatcgccct ggtgggcaag ctccaggata
     1441 aaaaggtcgt cgtcatcggc gacgtcatgc tggacgtcta cgtcaagggc acggtggccc
     1501 gcatctcacc cgaggccccc atccccgtgg tggagatcaa cgagaaggac cgcaagatgc
     1561 ccggcggggc cgccaacgtg gcctgcaaca tcaccgccct gggcggcgcc tccaccatca
     1621 tcggggtcat gggccgggac ctggcgggcc gggaactggt gcaggaactc aagcgccgga
     1681 aggtggacac gtcgggcatg ctggccctgg ccgaccgccc caccaccgag aagacccgcg
     1741 tcctggccca cacccagcag gtggtgcgca tcgaccggga aatgaaggcc cccctggacg
     1801 accgccagca ggac
//
LOCUS       DASTJJ010000218         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1254385, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000218 DASTJJ010000000
VERSION     DASTJJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254385"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..904
                     /locus_tag="VFR02_02660"
     CDS             215..904
                     /locus_tag="VFR02_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET9869388.1"
                     /translation="MCSRFALKSPPKTIAQLLGVEESLAWDPRYNVAPSQAIPTAVHP
                     LDGKKREMRMMHWGFTATWHQGGRLVVNLRSEDLQEKPLFQESFEKWRCLIPVDGFYE
                     WRHEARETRPFFIRLKSGRPFALAGLWAPQPSLGQTVDSCAILTTSPNETVKAIHERM
                     PVIVEGSDFGSWLDSKDIRDFDKIRELFKPYPADRMEAFEVDHWVNDPRHDGPRCLEP
                     FQNPGTLPLPF"
     gene            complement(912..1376)
                     /locus_tag="VFR02_02665"
     CDS             complement(912..1376)
                     /locus_tag="VFR02_02665"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9869389.1"
                     /translation="MPKSLVFIKKEISFEAAHYLHHPKWDRAKNLEVFRKCSGLRRDD
                     PGARDYPHGHSYRLAVTVSGAVRPDTGFVVDFRVLKEVLKREVYARFDHRFINKEVPP
                     FKDDPNLQPTAENLARVIWKLLEGPLKKEKARLTEVALWETPDSAAVYRGGK"
     gene            complement(1421..1819)
                     /locus_tag="VFR02_02670"
     CDS             complement(1421..1819)
                     /locus_tag="VFR02_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9869390.1"
                     /translation="MKNDIDPKINGFAKQVVDCAFEVHSTLGPGLLESIYETCLAHEI
                     ALRRLKVQRQVALPVSYKGMEFDDGLRIDLLIEGALIVEVKTVESLLPVHQAQLLTYL
                     KLTGCTLGLLINFHVPLIKLGIQRVVCSKE"
     gene            complement(1857..>2506)
                     /gene="nadE"
                     /locus_tag="VFR02_02675"
     CDS             complement(1857..>2506)
                     /gene="nadE"
                     /locus_tag="VFR02_02675"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET9869391.1"
                     /translation="DSRKLAEALGMGFENLPITPVFEAALGALRPLFGDRPPDLTEEN
                     LQARIRGLYLMALSNKFHRLVLSTGNKSELSVGYCTLYGDMCGGLAVISDVPKGMVYE
                     LARFVNDKAGRSVIPDAVFTKPPSAELRPDQTDQDSLPPYETLDAVLNLYIEKNQPAA
                     AIRRAGFEAPLVADLLGKVDRNEYKRRQMAPGLKVTTKAFGMGRRMPIAQKFKET"
BASE COUNT          451 a          777 c          794 g          484 t
ORIGIN      
        1 ggcttcccca gggtttgaaa ggggcccgca gggggaattt cccctgcgag cggagcggtt
       61 cgagcccgtc atgtccaccc atcgaacccc gccgcgtccg cggcggggtt ttttcattgg
      121 ggtgcggctg gaggagggac cgcgaagggg gaagggcttg ggcggcgccg ggggccccgt
      181 gggcctttcc ggtccggggt gttacactgg cgccatgtgc tcccggttcg ccctgaaaag
      241 cccgcctaag accatcgccc agctcttggg cgtggaagaa agcttggcct gggacccgcg
      301 ctacaacgtg gcgccttccc aggccatccc cacggcggtc catcccttgg acgggaaaaa
      361 acgggaaatg aggatgatgc attggggctt caccgccacc tggcaccaag gcgggcggct
      421 ggtggtgaac ctgcgttcgg aggacctgca ggaaaagccg cttttccagg aatccttcga
      481 gaaatggcgc tgcctcatcc cggtggacgg gttctatgaa tggcggcacg aggcccggga
      541 gaccaggccc ttcttcatcc gcctcaagag cgggaggccc ttcgccctgg ccggcctctg
      601 ggcgccccaa ccttccctgg gccaaacggt ggactcctgc gccatcctga ccacctcacc
      661 caacgagacg gtgaaggcca tccatgagcg catgccggtc atcgtggagg ggtcggattt
      721 cggttcatgg ctggattcga aggacatccg ggatttcgac aagatccggg agctgttcaa
      781 accctacccg gcggaccgca tggaggcctt cgaggtcgac cattgggtga acgacccccg
      841 gcacgacggc ccccggtgcc tggaaccctt ccagaacccg ggcaccctgc cgctgccttt
      901 ttaagccggg ttcattttcc gccccgatag acggcggcgc tgtccggcgt ttcccacagg
      961 gcgacctccg tcaggcgcgc cttttctttt ttcagcggcc cttccaaaag cttccagatc
     1021 acccgggcca ggttctcggc ggtgggctgc aggttggggt cgtccttgaa gggggggacc
     1081 tccttgttga tgaaacggtg gtcgaagcgc gcgtagacct cccgcttgag gacctccttg
     1141 agcacgcgga aatccaccac gaaccccgtg tccggccgca ccgcccccga cacggtcacc
     1201 gccagccggt aggaatggcc gtggggataa tcccgggccc cggggtcgtc ccggcgcagg
     1261 cccgagcatt tgcggaagac ctccaaattc ttcgcccggt cccacttggg atggtgcagg
     1321 taatgggcgg cctcgaagga gatctctttt ttgataaaga ccaaggattt gggcatggac
     1381 cccttttacc gcaaaggcgc aaaggcgcca aggaggattt tcattctttt gagcaaacga
     1441 cacgctggat gcccagcttg atcaatggaa catggaagtt gatcagcaac cccagggtac
     1501 atcccgtcag cttcaaatag gtcaataatt gggcctggtg gaccggcaaa agggactcca
     1561 ccgttttgac ttcaacgatc agtgcgcctt caatcaatag gtcgatcctc aatccatcgt
     1621 caaattccat ccctttgtac gaaacgggaa gagcgacttg acgctggacc ttgagccgtc
     1681 tcaaagcgat ctcgtgcgcc aaacaagttt cgtagatgct ttctaataat cccggcccca
     1741 aagtcgaatg gacctcgaaa gcacaatcca cgacctgttt ggcgaagccg ttgatcttgg
     1801 gatcaatgtc gtttttcatg gacttcgcgc ctttgcgtct ttgcggtgaa cgggccctat
     1861 gtttccttga atttttgggc gatgggcatc cggcggccca tgccgaaggc cttggtggtg
     1921 accttcaggc ccggggccat ctggcggcgc ttgtattcgt tgcggtccac cttgcccaac
     1981 aggtccgcca ccaggggcgc ctcgaacccc gcccggcgga tggcggcggc gggctggttc
     2041 ttctcgatat aaaggttgag gacggcgtcc agggtctcat agggcggcag gctgtcctgg
     2101 tcggtctggt cggggcggag ctccgccgag ggcggtttgg tgaagaccgc gtccgggatg
     2161 acggaccggc cggccttgtc gttcacgaaa cgcgccagct cgtagaccat ccccttgggc
     2221 acgtccgaga tcaccgccag cccgccgcac atgtcgccgt agagggtgca gtaacccacc
     2281 gaaagctcgc tcttgttgcc ggtggagagc accaggcggt ggaacttgtt ggacagcgcc
     2341 atcaggtaaa ggccccggat gcgggcctgc aggttctcct cggtcaggtc ggggggccgg
     2401 tcgccgaaca ggggccgcag ggcccccagg gccgcctcga agaccggcgt gatggggagg
     2461 ttctcgaagc ccatccccag ggcctcggcg agcttcctcg aatcct
//
LOCUS       DASTJJ010000219         1495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_643006, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000219 DASTJJ010000000
VERSION     DASTJJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643006"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFR02_02680"
     CDS             <1..318
                     /locus_tag="VFR02_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9869392.1"
                     /translation="RMDGCSELRIWATLILPLAKPALAVVALFQFIYSWNDFLGPLLF
                     LTRQKDFTLALGLQFYQSQNGGTQWHLLMAASVLASVPILLLFFLAQKTFIRGITLDG
                     LKE"
     gene            344..1024
                     /locus_tag="VFR02_02685"
     CDS             344..1024
                     /locus_tag="VFR02_02685"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET9869393.1"
                     /translation="MYGEYARSLAPKLLVSFGLLTALGFLGWILFGGGWSVFPASWGL
                     TGPGAGAGRAVLFALGLLYLAKVSATLDLILVRKMDWAEAATIAVWVNLIYLVYGLAG
                     AGPHAARGFVPFLGAGLFIAGAALNLASEWQRRRWKARPENKGRLYREGLFRRAVHIN
                     YFGENLSFVGFSLAAGPWWAFLIPALMAVLFIAANIPMLDAYLAKRYGDDFRDYAAQT
                     PKFIPFLY"
     gene            1061..>1495
                     /locus_tag="VFR02_02690"
     CDS             1061..>1495
                     /locus_tag="VFR02_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9869394.1"
                     /translation="MDIPSPLFLLNKPKGYVVSRSAQEGQKTVYELLPEWAFTEGWMP
                     VGRLDLESKGLLLFTQNSRLNDQLSRPRGNHKIYEVWVRGHVTKEHLQQVLKGIQTPE
                     GKLRAVHAEITGGAGPKTKLEVELDEGKNRHIRRMFGALKDQK"
BASE COUNT          238 a          501 c          471 g          285 t
ORIGIN      
        1 cgcatggacg gctgctcgga gctgcgcatc tgggccaccc tgatcctgcc gctggccaag
       61 cccgccctcg ccgtggtggc cctcttccag ttcatttaca gctggaacga cttcctgggc
      121 cccctgctct tcctgacccg gcagaaggac ttcaccctgg ccctggggct tcagttctac
      181 caaagccaga acggcggcac ccagtggcat ctcttgatgg cggcctcggt gctggcctcg
      241 gtccccatcc tgctcttgtt ctttttggcg cagaagacct tcatccgggg catcaccctg
      301 gacgggctga aggaataggc cggatctttt caaggaggtt cctatgtacg gggaatacgc
      361 gaggtccttg gcccccaagc tgctggtgag ttttgggctc ttgacggcgc tgggattttt
      421 gggatggatc ctcttcggcg ggggttggtc ggtctttccg gcgtcttggg ggctcaccgg
      481 acccggcgcg ggcgcggggc gggcggtgct tttcgccctg ggcctgctgt acctggccaa
      541 ggtctcggcc accctcgacc tcatcctggt acgcaagatg gactgggccg aggccgccac
      601 catcgcggtg tgggtcaacc tgatctacct ggtctacggc ctggcgggcg ccgggcccca
      661 tgccgcccgg gggttcgtgc ccttcctggg ggccgggctt ttcatcgccg gggccgcctt
      721 gaacctggcc tccgaatggc aacggcgccg ctggaaggcg cggcccgaga acaagggccg
      781 cctctatcgg gaaggcctct tccgccgggc ggtgcacatc aattatttcg gggagaacct
      841 gtccttcgtg ggcttttccc tggccgcggg cccctggtgg gccttcctca tccccgcctt
      901 gatggcggtg ctgttcatcg ccgccaacat ccccatgctg gacgcctacc tggccaagcg
      961 ctacggggac gacttccggg actacgcggc ccaaaccccc aagttcatcc ccttccttta
     1021 ttaagcgcca cgccggcccc ggccgtggta gaaaggtgcc atggacatcc cttccccgct
     1081 gttcctcttg aacaaaccca agggctacgt ggtcagccgc tcggcccaag agggccaaaa
     1141 gaccgtttat gagctgctgc ccgaatgggc cttcaccgaa ggctggatgc ccgtggggcg
     1201 cctggacctg gaatccaagg ggcttttgct cttcacccag aacagccggc tgaacgacca
     1261 gctttcccgc ccgcgcggca accacaagat ctacgaggtc tgggtgcgcg gccacgtgac
     1321 caaggaacac ctgcagcagg tcttgaaggg catccagaca cccgagggca agctgcgggc
     1381 cgtgcacgcc gagatcaccg gcggcgccgg ccccaagacc aaattggaag tggaactgga
     1441 cgagggcaag aaccgccaca tccggcgcat gttcggggcc ctgaaggacc agaag
//
LOCUS       DASTJJ010000220         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1254429, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000220 DASTJJ010000000
VERSION     DASTJJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254429"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFR02_02695"
     CDS             <1..573
                     /locus_tag="VFR02_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869395.1"
                     /translation="TATFTATPTVTQTGTDTPTFTRTLTPTATVSPTATVSLTPTPTH
                     TFTLSPTPSFTGTPTDTFTPTQTRTPTATRTITNTPGPTATATPTCPSPLFPNPVDFQ
                     ANHRPVQCPGGGNCVVFDCLAPGSTLKIYTIALSLVREYDSTGPTVTWDGLNGEANPV
                     ASGIYFYVVQDPSGSKTIGKFAVSRARVNP"
     gene            576..1538
                     /locus_tag="VFR02_02700"
     CDS             576..1538
                     /locus_tag="VFR02_02700"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9869396.1"
                     /translation="MAPSFLRRILCLCLFTFAGSQPVWAQMGGRGAFEFLKLSPFSRA
                     VGMGESYSAIGDDVAAIQYNPAGLANVLSNDFNFAYLSLYQGVSLESIAVAVPLDTQL
                     PAVGGTVAASVNLIQGGTFQRTDDLGNPAGSFTAGDDVITLAYARALGLIQVGASVKF
                     IQQQIDTVQSSLFAADAGVVVTPSFPGLRVGLTLKNIGAQDNTGYDLPMLFNASVSYR
                     RYEIFTYEDDGALTAETDVPVHPIEDPVGMRVGAEYNFKWVGSRATLRMGYKFLDESL
                     AGVGFTVGAGYALDLDGAVVFLDYAFAPEDVFGASNRISLSTKF"
BASE COUNT          260 a          564 c          475 g          273 t
ORIGIN      
        1 accgccacct tcaccgccac gccgacggtg acccagacgg ggacggatac gcccaccttc
       61 acccggaccc tcacgcccac ggccaccgtg agcccgacgg ccacggtgtc ccttaccccg
      121 acgcccaccc acaccttcac cctgagcccc acgccttcct tcacggggac gcccacggac
      181 accttcacgc ccacccagac ccgcacgccc acggcgaccc gcaccatcac caacacgccc
      241 gggcccacgg ccacggccac ccccacctgt cccagcccct tgttcccgaa tccggtggat
      301 ttccaggcca accatcggcc cgtgcagtgc ccgggcgggg gaaattgcgt ggtgttcgat
      361 tgcctcgcgc ccggctccac gctcaagatc tacacgatcg ccctgtcctt ggtgcgggaa
      421 tatgactcca cgggccccac ggtgacctgg gacggcttga acggcgaagc caacccggtg
      481 gcgtcgggca tttatttcta cgtggtgcag gacccctcgg ggtcgaagac catcggcaag
      541 ttcgcggtgt cgcgggccag ggtgaatccc tgaccatggc cccttctttc ctgcgccgga
      601 tcctatgcct gtgcctcttc accttcgccg gaagccagcc ggtctgggcc caaatgggcg
      661 gccggggcgc ttttgagttc ctgaagctct cgcccttttc ccgggcggtg ggcatgggcg
      721 agtcctattc ggccatcggc gacgacgtgg ccgccatcca gtacaacccg gccggcctgg
      781 ccaacgtgct cagcaacgac ttcaatttcg cctacctgtc cctctaccag ggcgtgagct
      841 tggaatccat cgccgtggcg gtccccctgg acacccaatt gcccgcggta ggcgggacgg
      901 tggccgcctc cgtcaacctc atccaaggcg ggacgttcca gcgcaccgac gacctgggga
      961 atccggccgg cagcttcacc gcgggggacg acgtcatcac cctggcctac gcgcgggccc
     1021 tggggctcat ccaggtgggg gcctcggtca agttcatcca acagcagatc gacacggtgc
     1081 aaagttcgtt gttcgcggcg gacgccgggg tggtggtgac gccgtccttc ccgggactgc
     1141 gggtgggcct gaccctcaag aacatcgggg cgcaggacaa cacgggatac gacctgccca
     1201 tgctcttcaa cgcttccgtc tcttatcgcc ggtacgagat cttcacctac gaggacgacg
     1261 gggccctgac ggcggagacc gacgtgccgg tgcaccccat cgaggacccg gtcggcatgc
     1321 gggtgggggc cgaatataat ttcaagtggg tggggagccg ggccacgttg cgcatgggtt
     1381 ataagttcct ggatgagtca ctggccgggg tggggttcac cgtgggagcc gggtatgccc
     1441 tggacctgga cggggcggtg gtcttcctgg attacgcttt cgcccccgag gatgtcttcg
     1501 gggcctccaa ccgcatctcg ctctccacga aattctaagc aaggcctgtt catgaaccgc
     1561 aaatcccaca tc
//
LOCUS       DASTJJ010000221         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1377941, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000221 DASTJJ010000000
VERSION     DASTJJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377941"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1014
                     /locus_tag="VFR02_02705"
     CDS             121..1014
                     /locus_tag="VFR02_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869397.1"
                     /translation="MKKLLFTLIAVLGLSSLAQADDFSLSGAQFAKAAPAAQPAMMMK
                     AETPAVTLFDGSGTFVQFQTGLDISFLDEIVNGTNGWKSFFPGSYISGTAARGGVASD
                     LSYGIHLDGASSLALNLGNVLTAPSVISATDPSNRTVFQADVAPSLYSASLVYSLNLA
                     QGAGSRIYFSLGGGWYHGIVSEATLGTSGPGGFGGTFTGDTVGGTLGLGGEIDLGSSF
                     GLGFTVNGRYAYFSRVSADKAGDASSNPGPYSLTEFTGPGGQHVLIPLDNADINSSSN
                     GYTAVDYSGIDAQFSLTYRFF"
BASE COUNT          226 a          395 c          346 g          255 t
ORIGIN      
        1 ccattccaaa aagagtgtcc ttttagggaa aaagcttact ttttttctcc cttgccacta
       61 aaattcccag accctcgggc cagtccatgg tccaaggaga atccccttag gagacgatcg
      121 atgaaaaagc ttttgttcac gctgatcgct gtcctgggcc tttcaagctt ggcccaagcc
      181 gatgacttct cgttgtccgg cgcccaattc gccaaggcgg cccccgccgc ccagccggcc
      241 atgatgatga aggccgaaac ccccgccgtg acccttttcg acggttccgg caccttcgtg
      301 cagttccaaa cgggcctgga catctcgttc ttggacgaga tcgtcaacgg caccaacggc
      361 tggaagagtt tcttccccgg gtcgtacatc tcggggaccg cagctcgggg cggcgtcgcc
      421 tccgacctgt cctacggcat ccacctggac ggggccagca gcctggccct caacttgggt
      481 aacgtcctta ccgccccttc cgtcatctca gcgaccgatc ccagcaacag gaccgtcttc
      541 caagcggatg tggccccctc gctctacagc gcttccctgg tctacagcct gaatctggcc
      601 caaggcgccg gcagccggat ctatttctcc ctgggcggcg gttggtacca tggcatcgtg
      661 agcgaggcca ccctggggac cagcggtccg ggcggcttcg gcggcacctt cacgggggat
      721 accgtgggcg gcacgttggg gttgggcggc gagatcgact tgggttcctc cttcgggctg
      781 ggcttcacag tcaatggccg ttatgcctat ttcagccgtg tcagcgcgga taaggcgggg
      841 gatgcttcca gcaatcccgg gccctattcg ctgaccgaat ttaccggtcc gggcggccag
      901 catgtcctga tccccctgga caatgccgac atcaattcca gcagcaacgg ctacaccgca
      961 gtggactatt cgggcatcga cgcccagttc agcctgacct accggttctt ctaagccggg
     1021 cgccgaaaaa caaaaagccg ggcgggatcc ccgcccggct ttttttatgc ccgccgcctc
     1081 aatattgaaa atacatctgc atggccacgc tcaccgaatt gctggcggtc gccgggaaag
     1141 gatccggcat gacacccgag aaatcgcaat aggcggcctg gttgttctgg ccgtagaaga
     1201 tgatgctgtt ggtcgtggtg tc
//
LOCUS       DASTJJ010000222         1973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1378003, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000222 DASTJJ010000000
VERSION     DASTJJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378003"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFR02_02710"
     CDS             complement(<1..932)
                     /locus_tag="VFR02_02710"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9869398.1"
                     /translation="MKAIAVTPKDKRVDVVDAPEPVLTHPSDVKLKMLDIGICGTDKE
                     ICAFDYGTPPPGKDRLIIGHESLGRVVEVGPGVKSFQPGDLVVMVVRRPCGRPECGPC
                     ADGRQDFCETGAFTERGINGLDGFMAEFVVDDEKYMNPVPEALRDIAVLAEPLTIAEK
                     GLEQMWQIQGRLPWDCDHAAAFGRGHCHNAVVLGAGPVGLLGAMLLRSQGFATFVYSK
                     EAADSPKAALAQRLGATFVSGSTTPLPELAARVGNVDLVYEATGASRLSYEMMKYLGV
                     NGIFIFTGVPGRKAPIEVDTDLLMRGQVLKNQVV"
     gene            1139..1378
                     /locus_tag="VFR02_02715"
     CDS             1139..1378
                     /locus_tag="VFR02_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET9869399.1"
                     /translation="MPEPNIAAKHPAEVQLEAGKTYAWCACGHSKKQPFCDGSHAAHG
                     FKPLVFQAEGSKTAHLCQCKHTKTPPFCDGTHKTL"
     gene            complement(1379..>1973)
                     /locus_tag="VFR02_02720"
     CDS             complement(1379..>1973)
                     /locus_tag="VFR02_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9869400.1"
                     /translation="AFLCVAPLGYVLAGFALGTTAALQGALLELACELPALAGLFYLA
                     QWLKECAGTDDLGRLRGLGRRAPGAASAFLLLGAGLVGVPLFGGFYAKLYVFEAALRQ
                     QQWWLVLGLAGAYGFSLLAWGVLAWRLFQAPTRETPIPRKSRPALEAVLALLALAGLA
                     MGLRHQALLRDVITPALPQAFQHWTPPVEDWDGKDVE"
BASE COUNT          348 a          712 c          630 g          283 t
ORIGIN      
        1 aacaccacct ggttcttgag cacctggccg cgcatcaaga ggtcggtgtc gacctcgatg
       61 ggggccttgc ggcccggcac gccggtgaag atgaagatgc cgttcacgcc caggtacttc
      121 atcatctcat aggaaagccg cgaggcgccg gtggcctcgt agaccaggtc cacgttgccc
      181 acccgggccg ccagctcggg gagcggcgtg gtggaacccg acacgaaggt ggcgcccagg
      241 cgctgcgcca gggcggcctt ggggctgtcg gccgcttcct tggaatagac gaaggtggcg
      301 aacccctggg agcgcagcag catggcgccc agcaggccca cgggccccgc gcccagcacc
      361 acggcgttgt ggcaatggcc ccggccgaag gcggccgcgt ggtcgcagtc ccagggcagg
      421 cggccctgga tctgccacat ctgctccagg cccttctcgg cgatggtcag gggctcggcc
      481 agcaccgcga tgtcccgcag ggcctccggc acggggttca tgtacttctc atcgtcgacc
      541 acgaactcgg ccatgaagcc gtccaggccg ttgatgcccc gctcggtgaa ggcgccggtc
      601 tcgcagaaat cctggcggcc gtccgcgcag gggccgcact cgggccggcc gcagggccgc
      661 cgcaccacca tcaccaccag gtccccgggt tggaaggact tcacgccggg ccccacctcc
      721 accacccgcc ccaacgattc gtggccgatg atgagccggt ccttgcccgg cgggggcgtg
      781 ccgtaatcga aggcgcagat ctccttgtcg gtcccgcaga tgccgatgtc cagcatcttg
      841 agcttcacgt cgctgggatg ggtgaggacg ggctcgggcg cgtcgacgac gtccacgcgc
      901 ttgtccttcg gggtgacggc gatggctttc atggggcctc cctgatggcc gaaagatgga
      961 tggggaaaac acccccacgg gtaatgacga cgggcggggt ccccaagggc ccccccatcc
     1021 taaccccaac ccccggggat ctttccagtc cgggtaccct tttcgccggg tctgtgtttt
     1081 actggctttg ccgcgttgcg cgccggtccc aggtttttac catctttaag gaggatccat
     1141 gcccgaaccc aatatcgccg ccaaacatcc cgccgaggtc caattggagg ccgggaagac
     1201 ctacgcctgg tgcgcctgcg gccattcgaa gaagcagccc ttttgcgacg gctcccatgc
     1261 cgcgcacggc ttcaagcccc tggtgttcca ggcggaggga tcgaagaccg cccacctttg
     1321 ccaatgcaag cacaccaaga ccccgccctt ctgcgacggg acccacaaga ccctttaact
     1381 attccacgtc cttcccgtcc caatcctcca ccgggggcgt ccagtgctgg aaagcctggg
     1441 gcagggcggg ggtgatgacg tcccgcaaca gcgcctggtg ccgcaggccc atggccaagc
     1501 ccgccagggc cagcagcgcc aggaccgcct ccagggcggg gcggctcttg cgcgggatcg
     1561 gggtttcccg ggtgggggcc tggaacaacc gccaggccag gacgccccag gccaggaggc
     1621 tgaacccgta ggccccggcc agccccagga ccaaccacca ttgctgctgc cgcaaagccg
     1681 cctcaaaaac gtaaagcttg gcgtagaaac ccccgaacaa ggggaccccc accaatccgg
     1741 cccccaggag caggaaggcc gacgccgcgc ccggcgcccg gcggcccaac ccccgcagcc
     1801 gtcccaagtc atccgtgccc gcgcattcct tcaaccattg ggccaggtaa aagaggcccg
     1861 ccagggccgg cagctcgcaa gccagttcca acaacgcgcc ctgcaacgcc gcagtggtgc
     1921 ccagggcgaa ccccgccagc acgtagccca agggggcgac gcagagaaaa gcc
//
LOCUS       DASTJJ010000223         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_521721, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000223 DASTJJ010000000
VERSION     DASTJJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521721"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2761)
                     /locus_tag="VFR02_02725"
     CDS             complement(<1..2761)
                     /locus_tag="VFR02_02725"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HET9869401.1"
                     /translation="MSFSLHLGPYQPFLEEELAGALAAFRAAEPFTPLAVLVPNRALV
                     RHLQEVLARRNGVLFNLRVLTLHQFLTEFTEEKWLREGMRLLPESLVPWALKGNARGK
                     GEKPGPFRAVEGMPGFHKTLRATLSDLRQGGFTPDNLAAASRALAKDKARRRLSEKLG
                     EFSRALQRHGDWKGAKGWKDLEDLYEDALTLPAPPSPVWTYGFYDASTLQQRVLEHLC
                     SATTSHWFIPYEEHPAFEYAVPFVVWAKSLGQVEGAGKYEDGKAGPLGRLQKRLFIEG
                     AAPASGDPEPAFEDSTVKVLLCPGEPREIREALRAVTQEAEKQKTHFSRCALLFRQLE
                     GYRRMIAPAFEGQGLPLAKAPAVFLMETPEAKSLLLLLECFERDFPRETLMDFLACPD
                     LSPEGFGLGEEDWDPHLWDQASKEAAVVEGKEAWLDRLQAYAKLRPAPEEGDDGIGGE
                     ASPLLAFRTKVLQALFDGARRFEKAGDWAQRAGTLFDLAEKWFLPSKAKEELTTLRRG
                     VEVLAKDLPQVAGNDLGALLSDLMEEVKLPWDLSEAGGILVSDLMQARGVPFDVVVLP
                     GLVEQGFPRVPRPDPLLLDEERRLLNGLASGQARVPEKAEGRAEEKLLFLLAARSAQK
                     CLILTASHLHPVTGAPRIPSSYLPEALRAVTGERRVKWDEAESIVRKVPVSDWARHEA
                     VRVDDLEEILGRFEEARQGNPLPAQAYADTKPFFEEARKLLKERQGSRKFTPYDGILS
                     GPEALEKLRQGHSLKDKTLSASRLETYAACPLRYFYRYVLGLKVHPEPERVFQLDPAE
                     KGNLMHSILEETLSRGVKEGWLKERDGEKALGVLDEETQKAFARLEKEGIPGSPALWE
                     WSRFSVRRDLERELRKVLAEKDWTPVDFEKAFGREGQPEVSFTTPAGSFRLEGFMDRV
                     DLSADG"
     gene            3017..>3163
                     /locus_tag="VFR02_02730"
     CDS             3017..>3163
                     /locus_tag="VFR02_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9869402.1"
                     /translation="MPREILIADAAGFCFGVKRAIEKAETLDRAFIFGSLIHNPQEVA
                     RLASL"
BASE COUNT          551 a         1023 c         1017 g          572 t
ORIGIN      
        1 ttccatcggc cgaaaggtcc acccggtcca tgaagccttc caggcggaaa ctgcccgccg
       61 gggtggtgaa ggaaacttcg ggctggccct cgcggccgaa ggccttctcg aaatccaccg
      121 gggtccagtc cttttccgcc aggaccttcc ggagctcccg ctccaggtcc cggcggacgc
      181 tgaaacggct ccattcccag agggcgggtg agccgggaat gccttccttc tcgagcctgg
      241 cgaaggcctt ctgggtctcc tcgtcgagga cccccagcgc cttttcgccg tcccgttctt
      301 tcagccaacc ttccttgacg ccccgggaaa gggtctcttc gaggatggaa tgcatcaggt
      361 tgcccttctc ggccgggtcg agctggaaga ccctttcggg ctcgggatgc accttcagcc
      421 ccagcacgta gcggtagaaa tagcgcaggg gacaggccgc gtaggtctcc aaccgggaag
      481 ccgagagggt cttgtccttt aaggagtggc cctggcgcag cttttcgagc gcctcaggac
      541 ccgaaaggat gccgtcatag ggggtgaact tgcggctgcc ctggcgttct ttgaggagct
      601 tgcgggcctc ctcgaagaag ggtttcgtgt ccgcgtaggc ctgggcgggc aaggggttgc
      661 cttggcgggc ttcctcgaag cggcccaaga tctcttccag gtcgtccacc cggaccgcct
      721 catggcgcgc ccagtccgac accggaacct tgcgcacgat ggactcagcc tcgtcccatt
      781 tcacccgccg ctcccccgtc acggcccgca gggcctcggg caggtagctg gaggggatgc
      841 ggggcgcccc ggtgacgggg tgcaggtggg acgcggtgag gatgaggcat ttttgggcgc
      901 ttcgcgcagc caaaaggaag agcagcttct cttcggcccg cccctcggct ttttcgggca
      961 cacgcgcctg gccggaagcg agcccattca ataggcgccg ctcctcgtcc aagagcaggg
     1021 ggtcgggccg gggcacccgg gggaaaccct gttccaccag gcccggcagc accaccacgt
     1081 cgaagggcac gccccgggcc tgcatgaggt ccgagaccaa gatcccgccc gcctcggaaa
     1141 ggtcccacgg gagcttcacc tcttccatca agtccgacag cagggcgccc aggtcgttcc
     1201 ccgccacctg gggcaggtcc ttcgcgagga cctccacccc gcggcgcagc gtcgtgagtt
     1261 cttccttggc cttggagggc aggaaccatt tttcggccag gtcgaagagc gtgccggccc
     1321 gctgggccca atcccccgct ttctcaaaac ggcgggcgcc gtcgaaaagg gcctgaagga
     1381 ccttcgtgcg gaaagccaac agcggggacg cttccccccc gatcccgtcg tccccctcct
     1441 cgggggcggg gcgaagcttg gcgtaggcct ggagccggtc gagccaggct tctttgcctt
     1501 ccaccaccgc ggcttccttg gaggcttggt cccagagatg cgggtcccaa tcctcctcgc
     1561 ccaggccgaa cccttcgggg gagagatcgg gacaagcgag gaagtccatc agggtctccc
     1621 gggggaaatc ccgctcgaaa cactcgagca acagcagcag ggacttggct tcgggcgttt
     1681 ccatgaggaa gaccgcgggc gccttggcca gcggcagccc ctggccctcg aaagccgggg
     1741 cgatcatgcg ccggtagccc tcgagctgcc ggaagagcag ggcgcatcgg gaaaaatggg
     1801 tcttctgttt ttcggcttcc tgggtgacgg cccgcagggc ttcccgtatt tcccggggtt
     1861 cccccggaca gagcagcacc ttcacggtgg aatcctcgaa tgcgggctcg gggtccccgg
     1921 aagccggcgc ggccccttcg atgaaaagcc gcttttggag ccgccccaag gggccggctt
     1981 tgccatcctc atatttaccc gcgccttcca cttggcccag ggacttggcc caaaccacga
     2041 agggaacagc gtactcgaag gccgggtgct cctcataggg gatgaaccaa tgggaagtcg
     2101 tcgccgagca aagatgctcc aggacccttt gttggagggt ggaagcgtcg tagaaaccgt
     2161 aggtccagac cggggaaggg ggcgccggga gggtcagggc gtcctcatag aggtcttcca
     2221 ggtccttcca tcccttggcc cccttccagt ccccatggcg ctggagggcg cgggaaaatt
     2281 cgccgagctt ttcggaaagc cggcggcggg ccttgtcctt cgccaaggcc cgggacgcag
     2341 ccgccaggtt gtcgggggtg aagccgccct ggcgcaggtc cgacaaggtg gcccgcaggg
     2401 tcttatggaa cccgggcatc ccctcgacgg cccggaaagg ccccggcttt tcgcccttcc
     2461 cccgcgcgtt gcctttcagg gcccaaggca ccagggactc gggtaaaagc cgcatgcctt
     2521 cccgaagcca tttttcctcg gtgaactccg tgaggaactg gtgcagggtc aggacccgca
     2581 ggttgaaaag gaccccgttg cgccgggcca ggacctcctg caggtggcgc accagggcgc
     2641 ggttgggcac caggaccgcc agcggcgtga agggctcggc ggcgcggaag gccgccaggg
     2701 ccccggccag ttcctcctca aggaagggct ggtagggtcc gagatggagg gaaaaactca
     2761 tgagggatcc tttaagaatt ttcaccgctc acgcccaagg gcggtcccgt tcccattttc
     2821 cgatgttagg gcagacgcca aggctccaag aaaagcttaa aaacattgtc tttggcagaa
     2881 agattcttaa acattgccgg tgattccttc aatattgaat cgtcattata tataatccat
     2941 ccccacaagg gttctgtgtt gggcttcctt ggcgtcttgg cgccttggcg gtgaaaatgc
     3001 tcttaaggac gttaagatgc cccgtgaaat tttgatcgcc gacgccgccg gattctgttt
     3061 cggcgtcaaa cgcgccattg agaaggccga aacgttggac cgggccttca ttttcggctc
     3121 gctcatccac aatccccagg aagtggcccg gctggcctcg ctc
//
LOCUS       DASTJJ010000224         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1254945, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000224 DASTJJ010000000
VERSION     DASTJJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254945"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1035
                     /locus_tag="VFR02_02735"
     CDS             <1..>1035
                     /locus_tag="VFR02_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9869403.1"
                     /translation="VAARAQEEADRYQTEQGQLSDMIVQKREENERLDQQNDQLSLAL
                     DDLEKKTSGLSQAKQEADAKVDAILEKRGGVAAEAEAVGEKVRASRGRFEEVQRDLHT
                     FEVQEAQIHVESRNIEEKLQVEYKVDLENPPVAVDESFDADEAEKESIELRAKIERLG
                     LVNMVAVEEYDELSQRYKFLAEQREDLLKAKDDLQKAIHKINLTSKELFTSTFATVQE
                     NFKDIFQRLFEGGRAELILIDEGDVLESGIEIVARPPGKRLQSVSLLSGGEKALTAIA
                     LLFALFMVKPSPFCLMDEIDAPLDDVNVSRFTHLLKEFSKRTQFVMVTHNKLSMETGD
                     VIYGVTMQES"
BASE COUNT          232 a          320 c          339 g          144 t
ORIGIN      
        1 aggtcgcggc ccgggcgcag gaagaggccg accgctacca gaccgaacag ggccaattgt
       61 ccgacatgat cgtgcaaaag cgcgaggaga acgagcggct ggaccaacag aacgaccaat
      121 tgagcctggc cctggacgac ctggaaaaga agaccagcgg cctgtcgcag gccaaacagg
      181 aagcggacgc caaggtggac gccatcctgg aaaaacgcgg cggcgtggcc gccgaggccg
      241 aagcggtggg cgagaaggtg cgcgcctccc ggggccggtt cgaggaggtc cagcgggacc
      301 tgcacacctt cgaggtgcag gaggcccaga tccacgtgga gtcgcgcaac atcgaggaaa
      361 aactgcaggt cgagtacaag gtggacctgg agaacccccc ggtggccgtg gacgagtcct
      421 tcgacgccga cgaggccgag aaggaatcca tcgagctgcg cgccaagatc gagcgcctgg
      481 gactggtcaa catggtggcc gtggaggagt acgacgagct gtcccagcgc tacaagttcc
      541 tcgccgagca gcgggaggac ctgctgaagg ccaaggacga cctgcagaag gccatccaca
      601 agatcaacct gacctccaag gagctcttca cctcgacctt cgccacggtc caggagaact
      661 tcaaggacat cttccagcgc ctcttcgaag gcggccgggc cgagctcatc ctcattgacg
      721 agggggacgt gctggaatcg ggcatcgaga tcgtggccag gcccccgggc aagcgcctgc
      781 agtcggtgtc cctgctttcc ggcggggaaa aggccctgac ggccatcgcg ctgcttttcg
      841 ccctgttcat ggtcaaaccc agccccttct gcctgatgga cgagatcgac gcccccctgg
      901 acgacgtcaa cgtgagccgc ttcacccact tgttgaagga gttcagcaag cgcacccagt
      961 tcgtcatggt gacgcacaac aagctgagca tggagaccgg cgacgtcatc tacggggtga
     1021 ccatgcagga atccg
//
LOCUS       DASTJJ010000225         1198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1745042, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000225 DASTJJ010000000
VERSION     DASTJJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745042"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>1198
                     /locus_tag="VFR02_02740"
     CDS             28..>1198
                     /locus_tag="VFR02_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9869404.1"
                     /translation="MNRSIIFLMVSIFLLAGCGKKDPKIYAQDGPVADGDMLIEPSLG
                     DAKNLMPPLVDEVGGGDIDALVFNGLLRYDPDFNLEPCLAQKWTVSKNGKVITYYLRH
                     GVKFHDGVEFTADDVLFTYKVYSDPTVNTPYGSNYKDIASVDKLDKYTVRVTYKEAYA
                     PALSTTFDTILPKHLLEGQDINKSDFNRHPIGTGPYKFVEWKTDQKIVLEANPDYWDG
                     APHIKKFVMRVIPDQSTEFLELLNGGVDALGAWLHGGLTPEQYARQIDTPKFKDYYNA
                     YRYDDLSYVYIGWNELNPLFKDKKVRQALTMALDRDAIIQNVIYGEGHVSTGPFPLHS
                     WANNPKVKPYPYDPAKALSMLKAAGWKPGKGGLLTRNGKPFQFTLMTNQGNVSRER"
BASE COUNT          262 a          404 c          342 g          190 t
ORIGIN      
        1 gggggaccga aaggaaaggc gcagcagatg aaccgttcga tcattttcct gatggtgtcc
       61 attttcctcc tcgccgggtg tgggaagaaa gaccctaaaa tttatgccca ggacggcccg
      121 gtggccgacg gggacatgct catcgagcct tcgctgggcg acgccaagaa cctcatgccg
      181 cccctggtgg acgaggtggg cgggggggac atcgacgccc tggtcttcaa cggcctgttg
      241 cgctacgacc ccgacttcaa cctggaaccc tgcctggccc agaagtggac ggtttccaag
      301 aacggcaagg tcatcaccta ctacttgcgc cacggcgtga agttccacga cggggtggaa
      361 ttcacggccg acgacgtgct cttcacctac aaggtctatt cggaccccac ggtcaacacc
      421 ccctacggca gcaactacaa ggacatcgcc tcggtggaca agctggacaa gtacaccgtg
      481 cgggtcacct acaaggaggc ctacgcgccg gccttgtcca ccactttcga caccatcctg
      541 cccaagcacc tgctggaagg gcaggacatc aacaagtccg acttcaaccg gcatcccatc
      601 ggcaccgggc cctacaagtt cgtggaatgg aagaccgacc agaagatcgt gctggaggcc
      661 aacccggact attgggacgg cgcgccccac atcaagaaat tcgtcatgcg ggtcatcccg
      721 gaccagtcca ccgagttcct ggagctgctc aacggcggcg tggacgccct gggcgcctgg
      781 ctgcacgggg ggctgacgcc cgagcaatac gcccgccaga tcgacacgcc caagttcaag
      841 gactattaca acgcctaccg ctatgacgac ctgtcctacg tctatatcgg ctggaacgag
      901 ctcaaccccc tgttcaagga caagaaggtc cgccaggccc tgacgatggc gctcgaccgg
      961 gacgccatca ttcagaacgt gatctacggc gagggccacg tgtccaccgg gcccttcccg
     1021 ctgcattcct gggccaacaa ccccaaggtg aagccctacc cctacgaccc ggccaaggcc
     1081 ctgtccatgc tcaaggccgc gggctggaag cccggcaagg gggggctgct cacccgcaac
     1141 ggcaagccct tccagttcac cctcatgacc aaccagggca acgtcagccg ggaacgca
//
LOCUS       DASTJJ010000226         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1745095, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000226 DASTJJ010000000
VERSION     DASTJJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745095"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFR02_02745"
     CDS             <1..325
                     /locus_tag="VFR02_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9869405.1"
                     /translation="LHDGMNLVAKEFVASSSNDRGVLVLSYFTGASRELEQALLVNPY
                     DSAAMAEALHQALTMAPEEQKTRLERMRMTVAENNIYSWALSILSDVARLAENVQPPS
                     GNGEA"
     gene            432..1115
                     /locus_tag="VFR02_02750"
     CDS             432..1115
                     /locus_tag="VFR02_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869406.1"
                     /translation="MQPIDGNIWTAPLAQLMDDLGTWAPSLLTALVVLVAGFALAWLL
                     RALCSIVFKRLDLDERLGNLWLFQIWSRSLHGRSPSQSMASFIFYLVIFVVILLAIRL
                     LGPEGGQAILSSLLGVIPRVLSFMLILFLGFLLAMFFSVLAQLALVGSGAQHPSFWGK
                     VIAWGTFGLTVMFSLEPLGLAGQFLTTLALMVLGAMGLAAAIALGLGCKDLAREFVIE
                     LLKGNDDDR"
     gene            1102..1878
                     /locus_tag="VFR02_02755"
     CDS             1102..1878
                     /locus_tag="VFR02_02755"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HET9869407.1"
                     /translation="MTTAEAQAVNSFVFKTSLESTQLTGRRAANLAEFEAGLREADGS
                     TLYHHTHRFYRVQSFLGAWNRSDFGLWVGNNLKEEAVAERMASLDTRDYPNLLELQKA
                     LLETLEPLRGDPDRWNRRVPPGLEFHFCRSVSLVLPTGYEVHNLEEFLRALSHIDLAC
                     LYYHLIEAPLHYHGDPRPFANDFSNWLADLGFTEEAQALAELNPYAGDLETLRTRVIG
                     VFRRDRMRAALRRVVDRLGREGAGEPAALWLKRWHQESKR"
     gene            1875..>2844
                     /locus_tag="VFR02_02760"
     CDS             1875..>2844
                     /locus_tag="VFR02_02760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9869408.1"
                     /translation="MSSPFAHYRPIIGAAEHDRIARLARYLKDQRVVMVNSTRNGGGV
                     AEILHNMVPMLNELGVETNWEVIEGNPGFFAATKTIHNALHGRAEALTPVMQKAYWDV
                     SESNAAKLADKMDRPLVYIHDPQPAGLLRHLRKGGQKWIYRCHVDVSTPYPPVWEFLS
                     AIMRDYDASVFSHPSFAQTLPNPQFLIAPSIDPLSDKNKPLPEDFVRKVLARYQLDAK
                     RPIITQVSRFDKLKDPLGVIQAFQKVKRTHPKAQLVLAGGAASDDPESAHILPAIQEA
                     AGKDPDIHVLDLPPFSDLDINALQRGSTVVLQKSLKEGFALTVSEAL"
BASE COUNT          456 a         1039 c          869 g          480 t
ORIGIN      
        1 gctgcacgac ggcatgaacc tggtggccaa ggaattcgtg gcctcctcca gcaacgaccg
       61 gggcgtgctg gtgctctctt atttcaccgg cgcgtcgcgg gagctggagc aggccctgct
      121 ggtgaacccc tacgactcgg cggcgatggc cgaggccctg caccaggccc tcaccatggc
      181 cccggaggag cagaagaccc ggttggagcg catgcgcatg accgtggccg agaacaacat
      241 ctacagctgg gccctgtcca tcctgagcga cgtggcccgc ctggccgaga atgtccagcc
      301 cccctcgggg aacggggaag cctaggccgg accggacccg gacgcctttt cccgcggttc
      361 ccccgcgggg tcccgggtct tccgtgcggc gagcatcacc aactctatag aagcatctta
      421 aggaggcaga catgcaaccg atcgacggga atatctggac cgcacccctg gcccagctca
      481 tggacgacct ggggacctgg gcccccagcc tgctgaccgc cctggtggtc ctggtcgccg
      541 ggttcgccct ggcctggctt ttgcgggccc tctgttcgat cgtcttcaag cgcctcgacc
      601 tggacgagcg cctggggaac ctctggcttt tccagatctg gtcgcgcagc ctgcacggcc
      661 gcagcccttc ccaatccatg gcttccttca tcttctacct ggtcatcttc gtggtcatcc
      721 tgctggccat ccggctcctg ggcccggagg gcggccaggc catcctctcc tccctgctgg
      781 gggtcatccc ccgggtcttg agcttcatgc tgatcctctt cctcggcttc ctgctggcca
      841 tgttcttctc cgtgctggcc caattggccc tggtgggctc gggggcccaa caccccagtt
      901 tttggggcaa ggtcatcgcc tggggcacct tcgggctgac cgtcatgttc tccctggagc
      961 ccttggggct ggccggccag ttcctcacca ccctggccct gatggtgctg ggcgccatgg
     1021 gcctggccgc cgccatcgcc ctggggctgg gctgcaagga cctggcccgg gaattcgtga
     1081 tcgaattgct gaagggcaac gatgacgacc gctgaggccc aggccgtgaa ttccttcgtc
     1141 ttcaagacct cgctggaatc cacccagctg accgggcgcc gggcggccaa tctggccgag
     1201 ttcgaggcgg gcctgcgcga ggccgacggc tccaccctgt accaccacac ccaccgcttc
     1261 taccgggtgc agtcgttcct gggcgcctgg aaccggtccg acttcggcct gtgggtgggc
     1321 aacaacttga aggaagaggc cgtggccgaa cgcatggcct ccctggacac ccgcgattac
     1381 cccaacctgt tggaactaca gaaggccctg ctggagaccc tggaacccct gcgcggcgac
     1441 ccggaccgtt ggaaccgccg ggtgccgccc ggcctggagt tccatttctg ccggtccgtg
     1501 agcctggtcc tgcccacggg ctacgaggtg cacaacctgg aggaattcct ccgggcgctg
     1561 tcccatatcg acctggcctg cctctactac cacctgatcg aggcgcccct gcattaccac
     1621 ggcgaccccc gccccttcgc caacgacttt tccaactggc tggccgacct gggcttcacc
     1681 gaggaggccc aggccctggc cgagctcaac ccctacgcgg gggacctgga gacgctgcgg
     1741 acccgggtca tcggcgtctt ccgccgcgac cgcatgcggg ccgccttgcg gcgggtggtg
     1801 gaccggctgg gccgggaggg cgccggcgaa ccggcggcct tgtggttgaa acgctggcac
     1861 caggagtcca aacgatgagc tcccccttcg cgcactaccg ccccatcatc ggcgccgccg
     1921 agcacgaccg catcgcccgc ctggcccgct acctcaagga ccagcgggtg gtcatggtca
     1981 attccacccg caacgggggc ggggtggccg agatcctgca caacatggtg cccatgctga
     2041 acgaactggg cgtggagacc aactgggagg tcatcgaggg caacccgggc ttcttcgcgg
     2101 ccaccaaaac catccacaac gccctgcacg ggcgggccga ggccctcacc cccgtcatgc
     2161 agaaggccta ttgggacgtg agcgaatcga acgcggccaa gctggccgac aagatggacc
     2221 ggcccctggt ctatatccac gacccccaac ccgccgggct gttgcggcac ctgcgcaagg
     2281 gcggccaaaa gtggatctac cgctgccacg tggacgtctc cacgccctac ccgcccgtgt
     2341 gggagttcct ttcggccatc atgcgcgatt acgacgcctc ggtcttctcc caccccagct
     2401 tcgcccagac cctgcccaac ccccagttcc tcatcgcgcc ctccatcgac ccgctgtcgg
     2461 acaagaacaa gcccctgccc gaggacttcg tgcgcaaggt gctggcccgc taccaactgg
     2521 acgccaaacg gcccatcatc acccaggtgt cccgtttcga caaactgaag gaccccctgg
     2581 gcgtcatcca ggccttccag aaggtcaagc gcacccaccc caaggcccag ttggtgctgg
     2641 ccgggggcgc ggcctccgac gaccccgagt cggcccacat cctgcccgcc atccaggaag
     2701 ccgccggcaa ggacccggac atccacgtgc tggacctgcc ccccttctcg gacctggaca
     2761 tcaacgccct gcagcgcggc agcacggtgg tgctgcaaaa gtcgctcaag gaagggttcg
     2821 cgctgacggt gtcggaggcc ctgt
//
LOCUS       DASTJJ010000227         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_644132, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000227 DASTJJ010000000
VERSION     DASTJJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644132"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFR02_02765"
     CDS             <1..217
                     /locus_tag="VFR02_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9869409.1"
                     /translation="AWNVLEYRHEKGYSQKMLADKAGISLRTVAYIEDYEEKFSPNIK
                     NVQAIAKALGVKFTDLFQTVDMTKAHG"
     gene            complement(238..522)
                     /locus_tag="VFR02_02770"
                     /pseudo
     CDS             complement(238..522)
                     /locus_tag="VFR02_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(662..1915)
                     /locus_tag="VFR02_02775"
     CDS             complement(662..1915)
                     /locus_tag="VFR02_02775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869410.1"
                     /translation="MTRSLKTLSAWTLTAGAFACAGSLWAGSAQDYDQAGLALFRAGQ
                     YEKAVQYFSNAVQADPNDAQAYEDMGNAYLKLGDKPNALNAYQSALKLKPGDTTLQAL
                     VDSLGGSGGGSEAGNGDVSASDSTDDSMQAPSYSPGTAPTTVIIEHHPLRRADVAPEP
                     VYHDGLPFMDHARIWSSVSLGYAYSSMTDLMNGVDATNQAIQNDGYSGQATFDRSGVG
                     LAFEVGFLLNPNSGIGLGVGWRRNSDLNENIDFNNGGDFQTINLQPQVYPLTLDYYLF
                     LPDAGGRFYLSAGVGYYFGRIHVDNNFDFSVANGSPDYDEFTGDLAASAPGFQLAVGR
                     EFAVTPRIGISLFAKGYYAKLSNFQGTLVDPYGNAGQFGLAILSDGSVDVPNTSQIGG
                     AGGARYATVDFTGFDVGFAVNFYHF"
     gene            complement(2044..>2337)
                     /locus_tag="VFR02_02780"
     CDS             complement(2044..>2337)
                     /locus_tag="VFR02_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869411.1"
                     /translation="QRYPLADWMDGFGVHQDYPMPLMRLGTFAYENGQFQVARDFFLK
                     ALKDSGGRYYEIYYNLGAAYGRLGRPDLARLCYEKALELKPSLKWAREKLGGA"
BASE COUNT          441 a          667 c          757 g          472 t
ORIGIN      
        1 ggcatggaac gtattggagt atcgccatga gaaaggatat tcccagaaga tgttggccga
       61 taaggcgggc atcagcctaa ggacggtcgc ttacatcgag gactatgaag aaaaattcag
      121 ccccaacatc aagaacgtcc aagcgatagc gaaggccttg ggggtgaagt tcaccgacct
      181 ttttcaaacc gtggatatga cgaaggcgca cggctaaatc gcgtagtata aaaatgttca
      241 cgaagcggtt tccgttaggt gggcattagg gttcagccct tttttatccc tgaaatagac
      301 gttcagcaga cctcccaaac gttgtttcct gacgatcttc ccggttgttt cttttttgcg
      361 taattccggc ccgggctcgg gtatccggcc ttccaggcct tggtggggac gttcgtggtt
      421 gtagtactcc gctaattccc gaagggcctt cctcagttgt tccttgctgg taatgatcat
      481 cctggacaaa cattgcccct tcagggtgcg cacgaatctt tcggcgtggg cgttgtaacc
      541 gagggtttga gatgggagtt ttcggcgggc cgcgcgggga cttcttgcgg ttcaatgaaa
      601 tggtcgaacg aaaaacaaaa aggcccgccc cctgggggac gggccttttt ttatgccggg
      661 atcagaagtg gtaaaagttc accgcgaagc ccacgtcgaa gcccgtgaag tccaccgtgg
      721 catagcgggc gccgccggcc ccgccgatct ggctggtgtt gggcacgtcc accgagccgt
      781 cgctcaggat ggccaggccg aactggcccg cgttgccgta ggggtccacc agggtgccct
      841 ggaagttcga gagcttggcg taatagccct tggcgaagag cgagatgccg atgcgggggg
      901 tcacggcgaa ttcccggccc accgcgagct ggaagcccgg ggcgctggcg gccaggtcgc
      961 cggtgaactc atcgtagtcg ggcgagccgt tggcgacgga gaaatcgaaa ttgttgtcca
     1021 cgtggatgcg gccgaaataa taccccacgc ccgcgctcag gtaaaagcgc ccgcccgcgt
     1081 ccggtaaaaa gaggtaatag tccagcgtca agggatagac ctggggctgc aggttgatgg
     1141 tctggaagtc ccccccgttg ttgaagtcga tgttctcgtt caggtcgctg ttgcgccgcc
     1201 agcccacgcc caaaccgatg ccgctgttgg ggttgagcaa aaagcccacc tcaaaggcca
     1261 gccccacgcc gctgcggtcg aaggtggcct ggcccgaata cccgtcgttc tggatggcct
     1321 ggttggtggc gtccacgccg ttcatcaggt cggtcatgga cgaataggcg tagcccaggg
     1381 agacgctgct ccagatgcgg gcgtggtcca tgaagggaag gccgtcgtga taaacgggtt
     1441 cgggggccac gtcggcgcgg cgcaggggat ggtgctcgat gatgacggtg gtgggggcgg
     1501 tgccggggga ataggaaggc gcctgcatcg aatcatcggt ggaatcggaa gcggacacat
     1561 cgccgttccc cgcctcactc ccacccccgc tcccgcccag gctgtccacc agggcctgca
     1621 gggtggtgtc cccgggtttc agcttgaggg ccgactggta ggcgttgagc gcgttgggtt
     1681 tgtcgcccag cttcaagtag gcgttgccca tgtcctcata ggcctgggcg tcgttggggt
     1741 cggcctggac ggcgttgctg aaatactgga cggccttctc gtattgcccg gcgcggaaaa
     1801 gggccaagcc ggcctggtca tagtcctggg cgctgccggc ccagaggctc cccgcgcagg
     1861 cgaaagcccc ggcggtcagg gtccaggcgg atagggtctt caaggatcgg gtcatggctg
     1921 cctccctcgg attccaacgc aaggcgttcg cggcttgaac ctatatcata aggtttagac
     1981 gccgggtttt tcatgacgtt acggcttttc ccgaaaggtc cgtgggccta tttttcaggg
     2041 aattcaggcc ccgccgagct tttcccgggc ccatttcagc gacggcttca actccaacgc
     2101 cttttcgtag caaagccggg ccaggtccgg ccggcccagg cgcccgtagg cggcccccag
     2161 gttgtaatag atctcgtaat aacgcccccc gctgtccttc agggccttga ggaagaaatc
     2221 ccgcgccacc tggaactgcc cgttctcgta ggcgaaggtg cccagccgca tgaggggcat
     2281 gggatagtcc tggtggacgc cgaaaccgtc catccaatcc gccagggggt agcgctg
//
LOCUS       DASTJJ010000228         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1256080, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000228 DASTJJ010000000
VERSION     DASTJJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256080"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFR02_02785"
     CDS             <1..1157
                     /locus_tag="VFR02_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET9869412.1"
                     /translation="NQATLSASKKILPQVTDVIQPAGGMTPEQLMTLVQAVLPTTNAA
                     TATVDTVRNVLIVHGTASDVAEYKQAMRDMKLEPGEESDRITKVVKLNYADPDTMMTI
                     LKTYLTPVGHVQEDPRTQDLVLWEVPENMGVLLELIKEMDVKAPQVLIESNLVEVSDE
                     KDLNLGISWAAVKTAGSDGVTSTANMLPTNLTGNITTPPMFNFGTVKDNTAINAELQA
                     LETKKKGKIISRPRIATASGVPAEIDTTQNVIVQTIQSTFVQGVGINQVTTFSQVPVP
                     INLKVTPRITDDGRITTQMAVTVTSITGTAPSGAPPPTTVETAQTTITTKNGETIVIG
                     GLVQDTISEEEDGVPLLQDIPILGALFQSKSHVHQKQELIIFVTPTLLED"
     gene            1306..>2262
                     /gene="aroC"
                     /locus_tag="VFR02_02790"
     CDS             1306..>2262
                     /gene="aroC"
                     /locus_tag="VFR02_02790"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9869413.1"
                     /translation="MSIRYLTAGESHGKGVLVLLEGLPAHLSVTREAINLELARRQGG
                     YGRGGRMAIEKDQVEILSGVRKGKTLGSPITLLVRNKDYENWEKIMSPDLDFPDKGNP
                     LQRPRPGHADLPGGIKYDHRDLRNILERASARETTARVAAGAVAKAFLEPFGIKVFSH
                     VLQLGKAAAVRTEKDPFRLQQKADRSPVRMVDPKAEKAAMALIDRAKTQGDTLGGIFE
                     VIVTGAPAGLGSHVQWDRKLDGRLAQAIMSIPAIKSVGIGAGPEVAERPGSQVHDEIV
                     RDERADRSTGLSRPTNNAGGLEGGITNGEDVRVSGFMKPISTL"
BASE COUNT          477 a          725 c          709 g          351 t
ORIGIN      
        1 ggaaccaggc gacgctcagc gcgtccaaga agatcctgcc ccaggtcacc gatgtgatcc
       61 agccggcggg agggatgacg cccgaacagt tgatgaccct ggtccaggcg gtgctcccca
      121 ccaccaacgc ggccaccgcc acggtggaca ccgtgcggaa cgttttgatc gtccatggga
      181 cggcctcgga tgtggccgaa tacaagcagg ccatgcggga catgaagctg gaacccggcg
      241 aggaatcgga ccggatcacc aaggtggtga agctcaacta cgccgatccg gacaccatga
      301 tgaccattct gaagacctac ctgacgcccg tgggccatgt ccaagaggac ccccggaccc
      361 aggacctggt gctttgggaa gtcccggaaa atatgggcgt ccttttggag ctcatcaagg
      421 aaatggacgt gaaggcgccc caggtgctga tcgagtccaa cctggtcgag gtctcggatg
      481 agaaagacct gaaccttggc atcagttggg ccgcggtcaa gaccgccgga tccgatgggg
      541 tgaccagtac ggccaatatg ttgcccacca acctgacggg caatatcacc acgccgccca
      601 tgttcaattt cgggacggtg aaggacaaca cggccatcaa cgccgaattg caggccctgg
      661 agaccaagaa aaaagggaag atcatctccc ggccgaggat cgcgacggcc agcggtgtgc
      721 ccgcggagat cgacaccacc caaaacgtca tcgtccagac catccagagc acttttgtcc
      781 aaggggtggg gatcaaccaa gtcaccacct tttcccaggt gcccgtgccc atcaacctga
      841 aggtcacgcc ccgtatcacg gatgacggcc gtatcaccac ccagatggcg gtgacggtca
      901 cctcgatcac gggaacggcg ccttccggcg ccccgccgcc cacgacagtg gagacggccc
      961 agacgaccat caccacgaag aacggcgaga ccatcgtcat cggcgggttg gtgcaggaca
     1021 ccatctcgga ggaagaggac ggcgttccct tgttgcagga cattcctatc ttgggggccc
     1081 tcttccagtc caagtcccac gtccatcaaa aacaggagct gatcatattc gtcacgccca
     1141 cgctcctcga ggattagcgg ggcttccgca gggccttgcg gcgttccgcc gccgcggggg
     1201 aacgaagagg gtttgacgcc cttagcgcct tggccacaag gcgctaattt tttttggccc
     1261 ggcttttccc gggcgtggcc gtaaaaacaa aatgggagtg ctccattgtc catccgttac
     1321 ctgaccgccg gcgaatccca cgggaagggg gtcttggtcc tgctggaggg cctgcccgcc
     1381 cacttatccg tcacccggga agccatcaac ctggaactgg cccgccgtca gggcggttac
     1441 ggccgcggcg gccgcatggc catcgagaag gaccaggtgg agatcctttc gggcgtccgc
     1501 aaggggaaga ccttgggctc ccccatcacc ctcctggtcc ggaacaagga ctacgagaat
     1561 tgggaaaaga tcatgtcgcc cgacctggac tttcccgaca agggcaatcc cctgcaacgg
     1621 ccccggccgg gccacgccga ccttccgggc ggcatcaagt acgatcaccg ggacctgcgc
     1681 aacatcctgg aacgggccag cgcccgggag acgaccgccc gggtggccgc cggcgcggtg
     1741 gccaaggctt tcttggaacc gttcgggatc aaggtcttca gccatgtcct gcagttgggg
     1801 aaggcggccg ccgttcggac cgaaaaagac cccttccgcc tccaacagaa ggccgatcgg
     1861 tccccggtgc gcatggtgga tccgaaagcg gagaaggcgg ccatggccct gatcgaccgg
     1921 gccaagaccc agggggacac gctgggcggc attttcgagg tcatcgtgac gggggccccc
     1981 gcgggcctcg gcagccacgt ccaatgggac cgaaagctcg acggccggct ggcccaggcg
     2041 atcatgtcga tccccgccat caaatcggtc gggatcggtg caggaccaga agttgccgaa
     2101 cggcccggct cgcaggttca cgacgaaatc gtgcgcgacg agagggccga tcgctcgacc
     2161 gggctctcga ggccgacgaa caacgccggg ggactcgaag gaggcatcac gaacggcgag
     2221 gacgttcggg tgtcgggctt catgaagccg atctcgacac tc
//
LOCUS       DASTJJ010000229         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1012821, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000229 DASTJJ010000000
VERSION     DASTJJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012821"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..456)
                     /locus_tag="VFR02_02795"
     CDS             complement(49..456)
                     /locus_tag="VFR02_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HET9869414.1"
                     /translation="MRAHISIDVKDIPGSTAFYRRVFGVEPQKQAADYAKFDLTAPAL
                     NFSMQSRPGRPASRVGHFGIEVDSPEELSRWQERLEAAGFLKRVETQTACCFARQDKL
                     WTEDPDGNAWEVFYVHEQLPVHASASLDGQCCP"
     gene            complement(453..851)
                     /locus_tag="VFR02_02800"
     CDS             complement(453..851)
                     /locus_tag="VFR02_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HET9869415.1"
                     /translation="MDRIQLSLQIGEVARQSGTAVDTIRYYEKKGLLDPPVRSAGGFR
                     LYPAGVVQRLLFIRKAQALGLTLKEIKRITCCGDQGLGPCCDLTVDLFSAKIREFEGK
                     IAELQGMKKKLKTVLGGWVGPTKRKSGRRA"
     gene            complement(941..1423)
                     /locus_tag="VFR02_02805"
     CDS             complement(941..1423)
                     /locus_tag="VFR02_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET9869416.1"
                     /translation="MGVWDRDYPYWLMKAEPDVYPVEQWKKDGVTFWDGVRNYTARNF
                     MRDRMKKGQGMLFYYSGAKPSGIIGVGEIVKEGYPDPSAFDPKDPHYDEKSDPKKPTW
                     YCVDVKYKKTLKAILPIDFLKKQPALKDMALFKYSRLSVQPVTQKEWDFIQKLIAAQE
                     "
     gene            complement(1540..1851)
                     /gene="glpE"
                     /locus_tag="VFR02_02810"
     CDS             complement(1540..1851)
                     /gene="glpE"
                     /locus_tag="VFR02_02810"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HET9869417.1"
                     /translation="MHDVKEIGLDEAQERLKAGQTFLDVRDEGSFQAGHVPGAVFLDD
                     RSVGDFMAKADKKAPVVVYCYRGHTSLGAAAYLMDNGFTDVMSLTGGFEAWRPTGKIE
                     S"
     gene            complement(1844..2629)
                     /locus_tag="VFR02_02815"
     CDS             complement(1844..2629)
                     /locus_tag="VFR02_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9869418.1"
                     /translation="MRKGTLSVPIITLNEEKNIARCLDSLKALKPFFDKVQAVVVDAG
                     SRDKTLALARARGAQVHTRAWKGFGDQKNWALSRCRGDWILSLDADEELTPALCAEIG
                     GLLKAPPPGVDGYFIKRRAFFLGKWIRHCGWWPDSQLRLIRKGKGRFTDEPVHEGLEV
                     AGETPELKEPMNHYTYDSIRQYLDKMNHYSGLAVLDVKKKKKLFWAYYLTLAPFFTFT
                     RMYLSRRGFLDGWHGFVVCGLSAFHDFAKYAKLWEKEVLRRHA"
BASE COUNT          453 a          859 c          838 g          534 t
ORIGIN      
        1 cgaaggtgcc caaggcctcc gcggccgcct gcttccaggc cttggccctc acgggcagca
       61 ctgtccgtcg agggaggcgg aggcgtggac cgggagctgc tcatggacat agaacacttc
      121 ccaggcgttc ccgtccgggt cctcggtcca aagcttgtcc tgccgggcga agcagcaggc
      181 ggtctgggtc tccacccgct tgaggaaccc ggcagcctca agcctttcct gccaccggga
      241 cagttcctcc ggcgaatcca cctctatccc gaaatggccg acccggctgg cgggacggcc
      301 cgggcggctc tgcatggaaa aattgagcgc cggggccgtg aggtcgaact tggcgtaatc
      361 cgccgcctgt ttctgcggtt ccacgccgaa cacccgccgg tagaaggccg tcgagccggg
      421 gatgtccttc acgtcgatgg agatatgcgc cctcatgccc gcctcccgga cttccttttc
      481 gttggaccga cccaaccgcc cagcaccgtc ttgagctttt tcttcatccc ctgcaattcg
      541 gcgatcttgc cctcgaactc ccggatcttg gcgctgaaga ggtcgaccgt caggtcgcag
      601 caggggccca ggccctggtc cccgcagcag gtgatgcgct tgatctcctt caaggtcagc
      661 cccagggcct gggccttgcg gatgaagagc agcctctgca ccaccccggc cggatagagc
      721 ctgaacccgc cggcgctacg gaccggcggg tccaggagcc ccttcttttc gtaatagcgg
      781 atggtgtcga cggcggtccc gctctggcgg gcgacctcgc cgatctgaag tgacagctgg
      841 atccggtcca atgggccccc tggtggaacg ataggataga ccctggagca tactccaggg
      901 tcaaggggat ttcgagggga ccggaaaaaa ggggggacgg ctattcttgc gcggcgatga
      961 gcttttggat gaaatcccat tccttctggg tcacgggctg caccgacagg cgggaatact
     1021 tgaagagggc catgtccttc agggccggct gtttcttgag gaagtcgatg ggcaggatgg
     1081 ccttgagggt cttcttgtac ttcacgtcca cacaatacca ggtgggtttt ttggggtcgc
     1141 tcttctcgtc gtaatgggga tccttggggt cgaaggccga ggggtcgggg tagccttcct
     1201 tgacgatctc ccccacgccg atgatgccgc tgggcttggc gcctgaatag taaaagagca
     1261 tgccctggcc cttcttcatg cggtcccgca tgaaattgcg ggccgtgtag ttgcgcacgc
     1321 cgtcccagaa ggtgaccccg tcctttttcc attgctcaac ggggtaaacg tcgggctcgg
     1381 ccttcatgag ccaataagga tagtccctgt cccaaacacc catagatcct cccgtcttaa
     1441 gacgaacctt tttcaccgca aaggcgcaaa gacgcgggga gatgccgggg ctttagccga
     1501 ctttgcgtct tcgcgccttt gcggtgaaaa ggttttgcct cagctttcga tcttccccgt
     1561 ggggcgccag gcctcgaagc cgccggtgag gctcatcacg tccgtgaagc cgttgtccat
     1621 caagtaggcg gcggcgccca ggctggtgtg gccccggtag caatagacca ccaccggcgc
     1681 ttttttgtcg gccttggcca tgaagtcccc cacgctgcgg tcgtccaaga agaccgcccc
     1741 cggcacatgc cccgcctgga aggacccctc gtcccgcacg tccaggaaag tctgcccggc
     1801 cttcaggcgt tcctgggcct cgtccaatcc gatctctttc acgtcatgca tggcgcctca
     1861 agacctcctt ttcccagagt ttggcgtatt tggcgaagtc gtggaaggcc gacaggccgc
     1921 agaccacgaa cccgtgccag ccgtccaaaa aaccccggcg cgagaggtac atccgggtga
     1981 aggtgaagaa gggcgccagg gtcaggtaat aggcccagaa tagcttcttc ttctttttca
     2041 cgtccagcac cgccagcccc gaatagtggt tcatcttgtc caggtattgg cggatggaat
     2101 cgtaggtgta gtggttcatg ggttccttca gttccggcgt ctccccggcc acctccaggc
     2161 cctcgtgcac gggttcgtcg gtgaaccggc ccttcccctt gcggatgagg cgcagttggg
     2221 aatcgggcca ccagccgcaa tggcggatcc acttgcccag gaagaaggcc cggcgcttga
     2281 tgaaatagcc gtccaccccc gggggcgggg ccttcaggag cccgccgatc tcggcgcaga
     2341 gcgcgggggt gagttcctcg tccgcgtcca acgacaggat ccagtcgccc cggcagcggg
     2401 acagggccca gttcttttgg tccccgaacc ctttccaggc ccgggtgtgg acctgggcgc
     2461 cccgggcgcg ggccagggcc agggtcttgt cgcggctgcc cgcgtccacc accacggcct
     2521 ggaccttgtc gaagaatggc ttcagggcct tgaggctgtc caggcagcgg gcgatgttct
     2581 tttcctcgtt cagggtgatg atggggaccg agagcgtgcc ttttctcacg ggcgcccccc
     2641 gtagcgccac gcgcggtaac gcaggtagaa accgctggtc ttct
//
LOCUS       DASTJJ010000230         1026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1256643, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000230 DASTJJ010000000
VERSION     DASTJJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256643"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..74)
                     /gene="ffs"
                     /locus_tag="VFR02_02820"
     ncRNA           complement(<1..74)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR02_02820"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            252..476
                     /locus_tag="VFR02_02825"
     CDS             252..476
                     /locus_tag="VFR02_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869419.1"
                     /translation="MTVKMIYGIFQWIFMALAFICGISAMLRVEAFSTQSTQKVRHHG
                     DQQALLWILTFLFAIAAFVAAALAVRHGLR"
     gene            873..957
                     /locus_tag="VFR02_02830"
     tRNA            873..957
                     /locus_tag="VFR02_02830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:907..909,aa:Ser,seq:gga)
BASE COUNT          170 a          338 c          321 g          197 t
ORIGIN      
        1 gccagagccc gcttaccctt gctaccttcc ggtcctgggg gagttcacag ggtgacgcca
       61 catgaggggc cgggaatcag cttacccgac ggacgcggga cgggcgaatc cgtgccgggg
      121 cagggaccgg cgcccgtgcc ataccaattt tcagggcggg gtggtacgtt cgcgagaggg
      181 acgctccttc tttaacagct gcgaaaaagg tcccgggccg acctgcccct gccgcccgag
      241 gccatgagga gatgacggtc aaaatgatct acggaatttt tcaatggata ttcatggcgc
      301 tcgcctttat ctgcggcata tccgccatgt tgcgcgtcga ggccttctcg acgcaaagca
      361 cccagaaggt gcgccaccac ggcgaccagc aggcgctgct gtggatcctg accttcctct
      421 ttgccattgc cgcttttgtt gccgccgccc tggcagtccg gcacggcctg cggtagtggg
      481 acacgacgcc ggtcaaggca ccctggcggg ccgcccggcc gcggctgcgg gcctttccgg
      541 gttcgtccgc ggccgcgcca aaccgcccat gtttcaactc gcgaaatatt tgccgcaata
      601 tgtcacagct gcgtgacaat gttgtaaagt tgcgaatgca cgccacccgc ggagggagca
      661 ccgcggcgga gtgcagggac aggcgccatg gcgccagtcc ccagcaagag ggtctgcctt
      721 ccgatggacc gcatgggaaa tgggggtagt ggagcttgaa cggggcccca ctacccctct
      781 ttcttgccct ccgccggcac cggcaggccc cgattcgtga cttcggcact tcgtcaggta
      841 ctcttgaacc tgccgtgttc gccacggcag ctggaggatt cgcctagcgg cctatggcgc
      901 acgcctggaa cgcgtgttgg gttaacgccc tcgggggttc aaatccccca ttctccgccg
      961 gtcaagaacc cggccccgca taccgcgggg ccgggttttt gctttttcgc cgccgtcacg
     1021 ttccct
//
LOCUS       DASTJJ010000231         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1257017, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000231 DASTJJ010000000
VERSION     DASTJJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257017"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFR02_02835"
     CDS             <1..652
                     /locus_tag="VFR02_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869420.1"
                     /translation="YPHHRSVTLTPWSLEEPERPLAPDPVEREALFGDAKLGFLYSGN
                     LSHPHEFYLTLKLARKMREKAVFTYSARGNRLDDLKKAWNPEDVNVRFTAFAPLDRLE
                     ARLSAPDAHLVSLKEAYRGVAVPSKFFGALAAGRPVLFEGSPRSAIARWIEEYGVGWV
                     LTEDSLRQVAEGLESFGADPAAKKALFKRCHRVYQEHFSRQAVLDRWADELRALMK"
     gene            703..>1267
                     /locus_tag="VFR02_02840"
     CDS             703..>1267
                     /locus_tag="VFR02_02840"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9869421.1"
                     /translation="MKNKKAFVTGVTGQDGSWLADLLIEKGYQVHGMVRRTSSFNTGR
                     VDHLYQDPHVNGRRFFLHYGDLSDSSGLFKLLSSIRPDEIYNLGAQSHVRVSFDQPEY
                     TGEVTGLGTLKLLEAMRAACPKARFYQASSSELYGGLDCPKKGYSEKNPFYPRSPYGV
                     AKLYSFWAAKNYREAYGLHISNGILFNH"
BASE COUNT          245 a          390 c          394 g          238 t
ORIGIN      
        1 ctatccccat caccgttcgg tgaccttgac gccttggtca ctggaagagc cggaaagacc
       61 cctggcgccg gatcccgtgg agagggaagc gcttttcggg gacgccaagc tggggttcct
      121 ctattcgggg aacctgagcc atccgcacga gttctacctg accttgaaac ttgcccgcaa
      181 gatgcgggaa aaggctgttt tcacctatag cgcccgaggg aatcgattgg acgacctgaa
      241 aaaggcctgg aaccccgagg acgtgaacgt gcgcttcacc gctttcgcgc cgctcgaccg
      301 tctggaggcg aggctttcgg cccccgacgc gcatttggtg agcctcaaag aggcctacag
      361 gggcgtggcg gtgccctcga agttcttcgg ggcgctggcg gcgggacggc ccgttctctt
      421 cgaggggagc ccccgttcgg ccatcgcccg atggatcgag gaatacggcg tcggatgggt
      481 gctgaccgag gacagcctgc gccaagtggc ggaaggcctg gaatccttcg gcgccgatcc
      541 ggcggcgaaa aaagcgcttt tcaagcgttg ccaccgggtc taccaggagc acttttcacg
      601 ccaagccgtg ctcgaccgtt gggccgacga attgcgggcc ttgatgaaat aaggggcctt
      661 cggtatcctc gtgccggaag cccgatccat gagaggaaac ccgtgaaaaa caagaaggcc
      721 tttgtcaccg gagtgaccgg acaggacggt tcctggctgg ccgacctgtt gatcgagaag
      781 ggctatcagg tgcacggcat ggtccgccgc acctcctcct tcaacacggg ccgcgtcgac
      841 cacctttacc aagaccccca cgtgaacggc cggcggttct tcctgcatta cggggacctg
      901 tcggattcct cgggcctgtt caaactgttg tcctccatcc ggccggacga gatctacaat
      961 ctgggcgccc aaagccacgt gcgggtctcc ttcgaccagc ctgaatacac cggcgaggtg
     1021 acgggcctgg gcacccttaa gctgttggag gccatgcggg cggcctgtcc gaaggcgcgc
     1081 ttttaccagg ccagcagttc ggagctgtat ggggggctgg actgcccgaa gaagggctac
     1141 agcgagaaaa acccctttta tccccgcagt ccctacggcg tggccaaact ttactccttc
     1201 tgggccgcca agaactaccg ggaggcctat ggactgcata tttccaacgg gatcttgttc
     1261 aaccacg
//
LOCUS       DASTJJ010000232         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1502541, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000232 DASTJJ010000000
VERSION     DASTJJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502541"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFR02_02845"
     CDS             complement(<1..589)
                     /locus_tag="VFR02_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET9869422.1"
                     /translation="MPQKHRWFRRAFFFAVSLYPAVLAADPSNAVVDPPEAASNTFSA
                     RTLQLDPVVVTANRLDTPADRVSSSMSVVTAGDLARTQARTALDALQTVPGISLTRTG
                     GPGGIASLYTRGGNDDATLVMLDGIPLNDPVGTSRSYNYLDQLSLDGIRQVEVVRGPQ
                     GALYGSAAMAGVVNLLTGDGQGPLGGSLRFEGGSYG"
     regulatory      complement(680..857)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(879..>1402)
                     /locus_tag="VFR02_02850"
     CDS             complement(879..>1402)
                     /locus_tag="VFR02_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9869423.1"
                     /translation="DVLETYTLLGKVLGHQAQAARARRRLEARLEAVQKGRAGREPVS
                     VLFVVGHNVGAIEGIYAAGGKSFVGQILRDSGGRNVLEDAAEAFPLVSKEALIREDPE
                     VIVDAMPGDEASEDTLKKARTAWARLPFLRAVRHHRVYFIRNDEMTIPGPTMVDLAEY
                     LSGIFGEAQRDLR"
BASE COUNT          236 a          487 c          461 g          218 t
ORIGIN      
        1 tgccgtagga accgccctcg aaccgcagcg aaccgcccag cgggccctgg ccgtccccgg
       61 tcaggagatt gaccacgccc gccatcgcgg cggaaccgta cagcgccccc tggggccccc
      121 ggaccacctc cacctggcgg atgccgtcca acgacaactg gtccaggtaa ttgtaggacc
      181 gggacgtccc cacgggatca ttgaggggga tgccgtccag catgaccagg gtggcgtcgt
      241 cgttgccgcc tcgggtgtag agcgaggcga tgccgcccgg tccgccggtc cgcgtcaggg
      301 agatccccgg gaccgtttgc agcgcgtcca gcgccgttcg ggcctgggtc cgggcgaggt
      361 ccccggcggt gaccacgctc atcgagctgg agacccggtc ggcgggcgtg tccaaccggt
      421 tggcggtcac caccacgggg tcgagctgca gggtccgggc ggaaaaggta ttggaggccg
      481 cctccggcgg gtccacaacg gcgttggaag gatcggcggc aaggacggcg ggataaagcg
      541 agacggcgaa aaagaaggcg cggcggaacc aacggtgttt ctgcggcatg gcccactccc
      601 cgaggcaaga tggcttcacc gggggtggaa aggagggccg tcaaatggga gggctggctc
      661 cgcttcttac acccgaagaa gcacgaacaa ccctcaaggc cggtctcctg gcttgggaac
      721 ctgccgcccg ccttcccggg tttccccagt ggctgcctcc atgaggaggc ctggacgaca
      781 gaactgcttc ccttacagtg gcgggtccgc gccggattcg caccggcttc ccgatcacct
      841 tgagtctggc aactagatac ccggctttcc gccgggtgtc aacgcaaatc cctttgggcc
      901 tccccgaata tccccgaaag gtactccgcc aggtccacca tggtgggacc cgggatggtc
      961 atttcatcgt tgcggatgaa atagacccga tgatggcgca ccgcccgcag gaacggcagg
     1021 cgggcccagg cggtccgggc ctttttcagg gtgtcctcgc tggcttcgtc ccccggcatg
     1081 gcgtccacaa tgacctccgg gtcctcgcgg atgagcgctt ccttggagac caagggaaag
     1141 gcctccgccg cgtcctccag gacgttgcgc ccgcccgaat cgcggaggat ctgaccgacg
     1201 aaggacttcc cgccggcggc gtagatcccc tcgatggcgc ccacattgtg ccccaccacg
     1261 aaaagcaccg agaccggctc gcgccccgcc cgcccttttt ggaccgcctc caacctggct
     1321 tccaggcgcc ggcgcgcccg ggccgcctgg gcttggtggc ccaacacctt ccccagcagg
     1381 gtataggtct ccagcacgtc ct
//
LOCUS       DASTJJ010000233         1290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1746785, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000233 DASTJJ010000000
VERSION     DASTJJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746785"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFR02_02855"
     CDS             <1..268
                     /locus_tag="VFR02_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869424.1"
                     /translation="NPSTDQISRYKNGVVQRVVIQAANAEDETVGTVLTFIKQSKEYL
                     GEEYHENNYKSFSHADLKKEYLKLFERVLKMKAQRGAGPQPPPQ"
     gene            513..>1290
                     /locus_tag="VFR02_02860"
     CDS             513..>1290
                     /locus_tag="VFR02_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9869425.1"
                     /translation="MLNFLLGLFSSDVAIDLGTATTLVYVKGRGIVLQEPSLVVVHKA
                     TNQILAVGNEAKTMLGRTPGSIVSIRPMKDGVIADFEVTERMLRHFISQVHNRKSLIR
                     PRIVVAIPSGCTEVEKRAVRDSAEQAGAREVFLVEEPMAAAIGSGLPVGEAQGNMIVD
                     IGGGTTEVAVISLTDIVYGKSIRVAGDEMDEAIITHIKKTYNILIGERTAEDIKIQIG
                     SAFPLEQELRMQIKGRDLVTGLPKSIELRSEEIREALEEPL"
BASE COUNT          266 a          413 c          390 g          221 t
ORIGIN      
        1 caaccccagc accgaccaga tcagccgcta caagaacggc gtggtccagc gggtggtcat
       61 ccaggccgcc aacgccgagg atgagacggt gggcacggtc ctgaccttca tcaagcagag
      121 caaggaatac ttgggcgagg aataccacga gaacaactac aagagcttct cgcacgccga
      181 cctcaagaag gaatatctca agctgttcga gcgcgtcctg aagatgaagg cccagcgcgg
      241 cgccggtccc cagcccccgc cgcaataatc tccccgaaaa tattcctttt tccccggttt
      301 ccccaaaggg cggcgcttcg gccggaaaag cccatattta ctgaaaaata ttggacagcc
      361 ctggggcctg tgttaaacta caacgccttt accgaagagt gatgaaacct ccgtgtttcc
      421 tcggttcatg accggggttt tttttttagg ggccgggtaa aggcgggccc cttccatacc
      481 accggccgcc tcgggcggcg aggagacgac acttgctgaa tttcctattg ggcttgtttt
      541 ccagcgacgt ggccatcgac ctgggcaccg ccaccaccct ggtgtacgtg aaggggcggg
      601 gcatcgtcct gcaggagccg tccctggtgg tcgtccataa ggccaccaac cagatcctgg
      661 cggtgggcaa cgaggccaag accatgctgg gccgcacccc cggttccatc gtctccatcc
      721 ggcccatgaa ggacggcgtg atcgccgact tcgaggtcac cgagcgcatg ctgcgccact
      781 tcatttccca ggtccacaac cgcaagtccc tgatccgccc ccgcatcgtg gtggccatcc
      841 cctcgggctg caccgaggtg gaaaagcggg ccgtgcgcga ttccgccgag caggcgggcg
      901 cccgtgaggt cttcctggtg gaggagccca tggcggcggc catcgggtcg ggcctgcccg
      961 tgggcgaggc ccagggcaac atgatcgtgg acatcggcgg gggcaccacc gaagtggcgg
     1021 tcatttcgct cacggacatc gtgtacggca agtccatccg ggtggcgggg gatgagatgg
     1081 acgaggcgat catcacccac atcaagaaga cctacaacat cctcatcggc gagcgcacgg
     1141 ccgaggacat caagatccag atcgggtccg ccttcccgct ggagcaggaa cttcgcatgc
     1201 agatcaaggg ccgcgacctg gtcaccggat tgcccaaatc catcgagctt cggtccgagg
     1261 agatccgcga ggccttggaa gagcccctca
//
LOCUS       DASTJJ010000234         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_524084, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000234 DASTJJ010000000
VERSION     DASTJJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524084"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFR02_02865"
     CDS             <1..725
                     /locus_tag="VFR02_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869426.1"
                     /translation="TWTSTFTPTLTPVPGVSFGAPQFTGTFDQNSGFGFSLPITITNS
                     TATSVSYTATLPSGMSFSGFTATAGGTAAGQTLSWPTLGPGVYTVAFNGTIAPTATGY
                     QAVNSTLFFTGGGPLNLAAGVTVVVPTATPTATVTPTSTPLILEPVTDPLPYPNPSTN
                     TDPISVQVSFGQPTGPVHLEIFTTAFRKIQDINEGSVEARPYTWKIYVTDKNSIPLAN
                     GLYYLVVSSPNGRGIGKLLVLR"
     gene            922..1668
                     /locus_tag="VFR02_02870"
     CDS             922..1668
                     /locus_tag="VFR02_02870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9869427.1"
                     /translation="MKFRMPLPAVLLAALWLAAGCGKKSPADQLPPPTGVMGKGVVSG
                     RVTLKGKIPAPVKLNMDADPACQAQHPGPVYDESVVADARGDLANVLVYVSSGAAVYA
                     PPSSPVTLIQKGCLYEPHVFGLQAGQPLDVFNDDPTLHNVHALAATNDGFNVGENEND
                     KTEQVFGRPEMPITFKCDVHSWMKGYGGVFSHPFFCVTGKDGAYKIPGLPAGTYTLTA
                     WQEKYGFSAPQTVTLKDGQSRKLDFSFTAP"
     gene            1725..>1924
                     /locus_tag="VFR02_02875"
     CDS             1725..>1924
                     /locus_tag="VFR02_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9869428.1"
                     /translation="MTDQDIYRPLLNRFALGLLGCTFLLLLAGALVTSTGSSLSVPDW
                     PTSYGSIVIPANKFNGGVVFEYG"
BASE COUNT          330 a          703 c          562 g          329 t
ORIGIN      
        1 cgacctggac ctccaccttc accccgaccc tcaccccggt gcccggggtg agctttggcg
       61 cgccccaatt caccgggacc tttgaccaga acagcgggtt cggtttcagc ctgccgatca
      121 ccatcaccaa cagcacggcc accagcgtct cctacacggc caccctgcct tcgggcatga
      181 gcttctcggg cttcaccgcc accgcgggcg gcacggcggc cgggcagacc ctgagctggc
      241 ccaccttggg tcccggcgtc tatacggtcg ccttcaacgg gaccatcgcg cccacggcca
      301 ccggctatca ggcggtcaac agcacgctct tcttcaccgg cggcggtccc ttgaacctcg
      361 cggcgggcgt caccgtggtg gtcccgacgg ccacgcccac ggccaccgtg accccgacct
      421 cgaccccgct catcttggag ccggtgaccg acccgcttcc ctatccgaac ccctcgacca
      481 acaccgatcc catctcggtg caggtgtcct tcggacagcc gacgggtccc gtgcacctgg
      541 agatcttcac cacggccttc cggaagatcc aggacatcaa cgagggctcg gtggaagcca
      601 ggccctatac ctggaaaata tatgtgacgg ataagaactc catccccttg gccaacgggc
      661 tctattacct ggtggtcagc tcccccaacg gccgaggcat cggcaagctc ctcgttcttc
      721 gctgatccag gtccgggccg ccgcgccgac ctcggcggac gaagacccaa aagcccgaca
      781 aaaacccaag gccccggcct tgggtttttt atttggggac ggggggtctt tttttcatcc
      841 aagacttggc tttgcgggcc ctttgaggca aagtaggaat ccggccatcg gcccttttat
      901 tttccctgag gagaaacccg catgaaattc cggatgcccc tgcccgccgt cctcctggcc
      961 gccctctggt tggccgccgg ttgcgggaag aaaagcccgg cggaccaatt gcccccgccc
     1021 acgggggtga tgggaaaagg cgtggtttcg ggccgggtga ccctgaaagg gaagatcccc
     1081 gcccccgtca agctcaacat ggacgccgat ccggcctgcc aggcccagca tcccgggccg
     1141 gtgtacgacg agagcgtggt ggccgacgcc cggggcgacc tggccaacgt gctggtgtac
     1201 gtgtcctcgg gcgcggcggt ctacgcgccg ccgtccagcc ccgtgaccct catccagaaa
     1261 ggttgcctct acgagcccca cgtcttcggc ttgcaggcgg gacagcccct ggacgtcttc
     1321 aacgacgacc ccaccctgca caatgtgcac gcgctggccg cgaccaacga cggcttcaac
     1381 gtgggggaga acgagaacga caagaccgag caggtcttcg gccgtcccga gatgcccatc
     1441 accttcaagt gcgacgtcca cagctggatg aagggctatg gcggggtctt ttcccatccc
     1501 ttcttttgcg tcacgggcaa ggacggcgcc tataagatcc ccggcctgcc cgcgggcacc
     1561 tataccctga ccgcctggca ggaaaagtac gggttctcgg cgccccaaac ggtcaccctg
     1621 aaggacgggc agtcccggaa gctggatttc agcttcaccg cgccttgagc ccccaagctt
     1681 ccgccgtccg ggcggacgcc catcccacgg aggtcccccc cgccatgacg gaccaggaca
     1741 tctaccggcc gctcctcaac cgcttcgcgc tggggctctt gggctgcacg ttcctgctgc
     1801 tcctcgccgg ggccttggtc accagcacgg gctcgagcct gtcggtgccc gactggccca
     1861 cgtcctacgg ctccatcgtg atccccgcca acaagttcaa cggcggggtg gtgttcgagt
     1921 acgg
//
LOCUS       DASTJJ010000235         1454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_279694, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000235 DASTJJ010000000
VERSION     DASTJJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279694"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFR02_02880"
     CDS             <1..627
                     /locus_tag="VFR02_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9869429.1"
                     /translation="SFTPKKHPNPELVPFEGKGVNYGVLSMEPFKGKDVLVVGGGDSA
                     IDWALMLEPIAHSVTLIHRRDEFRAHESSVQKLKASSCKVKLWYELRTVKGKDHVEEA
                     VIYENHTNQDETIKADFVVLNFGFQATLGFLKEWGLTLEKNKIPVTPKMETNIPGIYA
                     AGDIVTHPGKLDLIATGFAEGATAVNFAKTYINPHEKAEPGHSSNLKW"
     gene            750..>1454
                     /locus_tag="VFR02_02885"
     CDS             750..>1454
                     /locus_tag="VFR02_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869430.1"
                     /translation="MAVDVKKHRGWFFTLRRDAWWLEPLFYIALFGGFTVYATWAALN
                     PYGPQGRILYQWGPYLSPMFSPFFNPAWLHHLGLLGTPALLVLWAPGGFRGTCYYYRK
                     AYYRSIFLNPPGCAVGEGCVKYEGETKFFLFQNLHRYFMYIALLFIVILSWDAVLAMM
                     WPVNGIGPDGQSLPGGRQFGVGVGTLVMTLNAILLGGYTLGCHAFRHLVGGGLDFLRV
                     KRNSPVRFFLWSVVTHL"
BASE COUNT          261 a          470 c          431 g          292 t
ORIGIN      
        1 tccttcaccc ccaagaaaca cccgaacccc gagctggtgc ccttcgaggg caagggcgtc
       61 aactacggcg tgctttccat ggagcccttc aagggcaagg acgtgctggt ggtgggcggc
      121 ggcgactccg ccatcgactg ggccctgatg ctggagccca tcgcccactc ggtgaccctc
      181 atccaccgcc gcgacgagtt ccgcgcccac gagtcctccg tgcagaagct gaaggcctcc
      241 tcctgcaagg tgaagctctg gtacgagctc aggaccgtca agggcaagga ccacgtcgag
      301 gaagcggtga tctacgagaa ccacaccaac caggacgaga ccatcaaggc cgacttcgtg
      361 gtgctgaact tcggcttcca ggccaccctg gggttcctga aagaatgggg attgacgctg
      421 gagaagaaca agatcccggt gacccccaag atggagacca acatcccggg catctacgcg
      481 gcgggcgaca tcgtcaccca tccgggcaag ctggacctca tcgccaccgg tttcgccgag
      541 ggcgccacgg ccgtcaactt cgccaagacc tacatcaacc cccacgaaaa ggccgagccg
      601 ggacacagtt cgaacctgaa gtggtagtct tcaaaaacag ttaggaatga ggagttagga
      661 gtgaggggtt caagtcgaaa ggcttgagcc cggtgttcga cttctaactt ccaactctta
      721 actcctaact tgtgttgagg gggttcggga tggctgtcga cgtcaagaaa caccggggct
      781 ggttcttcac cctgcgccgg gatgcctggt ggttggaacc cctcttttat atcgcccttt
      841 tcggcggttt cacggtctac gccacttggg cggccttgaa tccctacggc ccccagggcc
      901 ggatcctcta ccaatggggg ccctaccttt cccccatgtt ctcccccttc ttcaaccccg
      961 cctggttgca ccatttgggg cttttgggga cgcccgccct gctggtgctt tgggcgccgg
     1021 gcggtttccg gggcacctgc tattactacc gcaaggccta ttaccgctcc atcttcctca
     1081 atccgccggg ttgcgcggtg ggtgagggct gcgtgaagta cgagggcgag accaagttct
     1141 tcctcttcca gaacctgcac cgctatttca tgtacatcgc gctgctcttc atcgtgatcc
     1201 tgtcctggga cgcggtgttg gccatgatgt ggccggtcaa cggcatcggc ccggacggcc
     1261 agtccctgcc cggcggccgg caattcggcg tgggcgtggg caccttggtc atgaccctga
     1321 acgccatcct gctggggggc tataccctgg gttgccacgc cttccggcac ctggtgggcg
     1381 gcggcctcga tttcctgcgg gtcaagcgca acagcccggt gcggttcttc ctctggagcg
     1441 tggtcaccca cctc
//
LOCUS       DASTJJ010000236         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1013811, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000236 DASTJJ010000000
VERSION     DASTJJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013811"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFR02_02890"
     CDS             <1..664
                     /locus_tag="VFR02_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869431.1"
                     /translation="TPTFTDTPTPSPTPTVTSTPSSALQVVQIFDPRGEMVRQLTGTL
                     LFADPDRLTMTLSGFTPAPSGQGGSVTISLDGQVVAVWDGLDSLDHPVPNGVYQVLVE
                     EKAGGESYFYSQNLSVEELSRPVGSSLTAWPNLAHEGDTIRFTAQIAGQPAQAPALLR
                     IYSLSGELVRTIGFSGGQALWNLDNAGNRSVASGLYIALLAGQDAFGRPVHQSLRVMV
                     IR"
     gene            949..>1314
                     /locus_tag="VFR02_02895"
     CDS             949..>1314
                     /locus_tag="VFR02_02895"
                     /inference="COORDINATES: protein motif:HMM:NF037833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869432.1"
                     /translation="MRPLVFLIPSIFFLSLSSASAQTCPSWVTVGNASGSGTAVTLTQ
                     PLGGQSGACWNSTRIDLTQDFNLTFTAYLGASTAGADGIDFVLQDDPRGTAAISGNGQ
                     PCGSCKGYSGVSPIAPSVAF"
BASE COUNT          223 a          420 c          372 g          299 t
ORIGIN      
        1 gacccccacc ttcacggaca ccccgacgcc ctcgcccacg ccgaccgtga cttcgacgcc
       61 ctcttccgcg ctgcaggtcg tccagatctt cgatccccgc ggcgagatgg tccgtcaatt
      121 gacggggacc cttcttttcg ccgatccgga ccggttgacc atgaccctct cgggcttcac
      181 gcccgcccca tcggggcagg ggggaagcgt gaccatctcg ctcgacgggc aggtggtggc
      241 ggtctgggat gggttggaca gcctggacca tccggtgccc aatggtgtct accaagtgct
      301 ggtggaggaa aaggccggcg gggaaagtta tttttattcg cagaacctgt cggtcgagga
      361 gttaagccgc ccggtgggga gctccttgac ggcttggccc aacctggccc acgaggggga
      421 cacgatccgg ttcacggccc agatcgcggg gcaacccgcc caggccccgg cgctcttgag
      481 gatctattcc ttatcgggcg aattggtgag gacgatcggc ttctcaggcg gccaagcctt
      541 gtggaatttg gataatgccg ggaaccgatc ggtggcgtcc ggcctttata tcgccctatt
      601 ggccggccaa gatgcctttg gccgccccgt ccaccaaagc cttcgggtca tggtgatccg
      661 ttagcatcct ttcctaattc ccatccagaa ggccctagaa accctttaaa aatagggata
      721 atttaaggtt aaacttttac ctagaatttt tattggtttg tggtaatctc atggacggtt
      781 cgggttaatt gtttaacatt aaacctcttt ttctacatgt ttagaagtgg tcgtgaggtg
      841 ggtcttttgg tacaaccggg catgtttttg ctaagcgcca tctttttgtc cgccctctgc
      901 aaatgggccc ctcgtttgaa cgtgttttcc aagacgttcc ttgcccccgt gcgccctctg
      961 gtgttcctga tcccctcgat ctttttccta tccctgtcct ccgcttcggc ccagacctgt
     1021 cccagttggg tgaccgtggg caacgcgtcc ggatcgggga cggccgtcac cctgacccag
     1081 ccccttggcg ggcagagcgg cgcctgctgg aactccaccc gcatcgacct gacccaggat
     1141 ttcaacctca ccttcaccgc ctacctgggc gccagcaccg ctggcgcgga cggcattgac
     1201 ttcgtcctgc aggacgaccc ccgcggtacg gcggccatca gcggcaacgg acagccctgc
     1261 ggatcctgca agggctactc gggcgtcagc cccatcgccc cctcggtggc attc
//
LOCUS       DASTJJ010000237         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_158183, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000237 DASTJJ010000000
VERSION     DASTJJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158183"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1181)
                     /gene="nuoH"
                     /locus_tag="VFR02_02900"
     CDS             complement(117..1181)
                     /gene="nuoH"
                     /locus_tag="VFR02_02900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010278996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET9869433.1"
                     /translation="MGEKIVGWVGGLLHGLYGFLRLTPFAGVLDTVVEAVAMFLFVAV
                     FVMFLVWMERKVSAHMQSRIGPNRVGPFGLLQTLADAIKLTLKEDVKPQRRDKVPYFI
                     APIVAFVATFMGMVVVPFDKGVVVQDLNVGLLYVIAITGFTVVAVLTAGWSSNNKYAL
                     LGGFRSAAQIVSYEIPLVLSLVPVIMVSGTLSLQAIVEYQARAGYWFVAPQLAAFLIF
                     LTSATAEANRAPFDIPEAESELIGGFHTEYSGVKFAMFFLGEYIAMVVSASVATAVFL
                     GGWLSPAFLGFLHLPGFLWFILKVLFVIYLIMAFRWTFPRLRVDQLMDFGWKVLIPLS
                     ILNILATGFVLLAQGAGKGM"
     gene            complement(1208..>1469)
                     /locus_tag="VFR02_02905"
     CDS             complement(1208..>1469)
                     /locus_tag="VFR02_02905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9869434.1"
                     /translation="GPVEAKMPPVLKAPAGGEVYSRIESSRGEMGVYLVGDGTAKPWK
                     AKFRCASFNNVFVLDQLTRGWKLADIMAVLGSMDMIVPEVDR"
BASE COUNT          315 a          478 c          452 g          224 t
ORIGIN      
        1 ccggatactg ttgggtgttg gtcttcttga aaaaataccg gatggtcacc gagaggccgc
       61 ccagcaggtt ccagaagccc cgcgcgatgc cgacgaaata gctcaccagg cccatgtcac
      121 atccccttcc cggcgccctg ggccagcagc acgaagcccg tcgccagaat gttgaggatg
      181 gagagcggga tcagcacctt ccacccgaaa tccatcaatt ggtccacccg aagccgcggg
      241 aaggtccagc ggaaggccat gatgaggtag atcacgaaaa ggaccttcag gatgaaccac
      301 aggaagcccg gcaggtgcag gaaacccaaa aaagccgggc tcaaccaccc gcccaggaag
      361 accgccgtgg ccacggaggc cgacaccacc atggcgatgt actcgcccag gaagaacatg
      421 gcgaatttca cgcccgagta ctcggtgtgg aacccgccga tgagctcgga ctcggcctcc
      481 gggatgtcga agggggcccg gttggcctcg gcggtggccg aggtcaggaa gatgaggaag
      541 gccgccagtt ggggcgccac gaaccaataa ccggcccggg cctggtattc cacgatggcc
      601 tgcaggctca aggtgccgga caccatgatg accgggacca gggacagcac cagggggatc
      661 tcgtagctga cgatctgggc cgcggaccgg aaaccgccca agagggcgta cttgttgttg
      721 gaactccatc cggccgtgag gacggccacc acggtgaagc ccgtgatcgc gatgacgtac
      781 aacagcccca cgttcaggtc ctgcaccacc acgcccttgt cgaagggcac caccaccatc
      841 cccatgaagg tggccacgaa ggccacgatg ggggcgatga aataggggac cttgtcccgg
      901 cgctggggct tgacgtcctc tttcagggtc agtttgatgg cgtccgccag ggtctgcagg
      961 aggccgaagg ggcccacccg gttggggccg atgcggcttt gcatgtgggc ggagacctta
     1021 cgctccatcc agaccaggaa catgacgaag accgcgacga agaggaacat cgccacggct
     1081 tccaccaccg tatccaatac gcccgcgaag ggcgtgaggc gcaggaaccc gtacaggccg
     1141 tgcaggagcc cccccaccca tccgacgatc ttttcaccca tggtcattcc atcccattcg
     1201 aaaattgcta gcggtccact tcgggaacga tcatatccat gctgccgagc accgccatga
     1261 tgtcggccag cttccagccc cgggtcaact ggtccagcac gaagacgttg ttgaaggacg
     1321 cacagcggaa cttggccttc cagggtttgg ccgttccgtc ccccaccagg tacacgccca
     1381 tctccccccg ggaggactcg atgcggctgt acacttcccc gcccgcgggc gccttcagga
     1441 cggggggcat tttggcctcc accgggccc
//
LOCUS       DASTJJ010000238         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1747317, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000238 DASTJJ010000000
VERSION     DASTJJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747317"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..>1137
                     /locus_tag="VFR02_02910"
     CDS             493..>1137
                     /locus_tag="VFR02_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HET9869435.1"
                     /translation="MSSSRKQVALSIGQEVPPVENYKSEPGPEIFGSLVFNDKAQKQF
                     LSKDVYKKLRATTQKGEPLDHSIADQVAQGMKEWALSHGATHYTHWFIPMTGLSAEKH
                     DSFMEPTPEGGAIAEFDGKTLIKGEPDASSFPSGGTRATFEARGYTAWDPTTPAFIQV
                     EVNGATLTIPTAFLSWTGEALDKKTPLLRSMEALSNQALRILRLFGNNEAKKVFT"
BASE COUNT          216 a          378 c          332 g          211 t
ORIGIN      
        1 ggaggggccc aaagggtctt cttcgcctgg ggcgtgggcg gccagtatct cttcgtggtc
       61 ccgggccggg ggttggtctg cgtggtcacc gggggcaact ataaggatgg tcccttgggc
      121 gcccggggcc tcgccctttt ccaagaccac gtgctgggcg ccttgcccgc gcggtgaaac
      181 ggtaaaggcc cggattcccc ttcttttcgt cccctcttcc cggtccggcg gcctacaaaa
      241 ttgtccgggc gttataaaag cgttaaaaaa acggaaagcc ccggtttttc ctgataaaaa
      301 tccggatggc tgcatagaat acgaatccgg ccgaacctcc acgcctttcc aacgcccgcc
      361 gagcccgtcc tttttggcga cggggatgtg tttttcccct gatcgggggc cgttcccgga
      421 acggcccggt ttttgctggt tcttggatgt ccgccgttgg gttgtccgaa aattcatcaa
      481 ggagaagaat ccatgtcgag ttcccgcaag caggtcgcct tgtccatcgg tcaggaagtg
      541 ccgccggtgg aaaattataa gtccgaaccg ggaccggaga tcttcggctc cctggtcttc
      601 aacgacaagg cccaaaaaca gttcctctcg aaggacgtct acaagaagct gcgggccacc
      661 acccagaagg gcgagccgct cgaccactcc atcgcggacc aggtcgccca gggcatgaag
      721 gaatgggccc tgtcccatgg cgccacccac tacacccact ggttcatccc catgacgggg
      781 ttgtccgccg agaaacacga ttccttcatg gaaccgaccc cggagggcgg cgccatcgcc
      841 gagttcgacg gcaagaccct catcaagggc gagccggacg cctcctcctt cccctcgggc
      901 ggcacccgcg ccaccttcga agcccgcggc tacaccgcct gggatccgac cacccccgcc
      961 ttcatccaag tggaggtcaa cggcgcgacc ttgaccatcc cgacggcgtt cctgagctgg
     1021 accggcgagg cgctcgacaa gaagacgccg ctgctgcgct cgatggaagc gctgtcgaac
     1081 caggccctgc gcatcctgcg cctgttcggc aacaacgaag ccaagaaggt cttcacc
//
LOCUS       DASTJJ010000239         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_280080, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000239 DASTJJ010000000
VERSION     DASTJJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280080"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFR02_02915"
     CDS             complement(<1..228)
                     /locus_tag="VFR02_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869436.1"
                     /translation="MKDVVIVDGGRTPIGNLGGALKDVTPQDMAATVMKELIKRNNLK
                     PSEIDEVILGCVGQFSDAPNVARVASLLAGIP"
     gene            complement(342..>1613)
                     /locus_tag="VFR02_02920"
     CDS             complement(342..>1613)
                     /locus_tag="VFR02_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9869437.1"
                     /translation="EVGEACGLKRVWVTRINDYFPIPLKWLYPLKAKKEKTWVDWPTG
                     PLFRSFHHEVEKAPATPQRRPLGMDETALLQYTGGTTGVSKGVIMTHRNMVANAYQCK
                     SWFPGLPEGKEVFMLAIPIFHSYGMTAGMTFAISMAASMVLVPKFDVVQVLKAVDKYK
                     VSIFPGVQAIYVAINNYPEVGKYDVHSIKACISGAGPLHQEVQKKFEALTGGKLVEGY
                     GLTEASPVTHCNPVYGKRKLGMIGLPFPDTDAKIMDVETGTQQMGVKETGELVIKGPQ
                     IMQGYWNHPEETKMVLRDGWLYTGDIGYMDEDGFFAIVDRKKDMVKVGGENVYPRDVE
                     EVLFQNEKVLDCVVAGVPDEKLVDKIKAYIVLKPGQAATAPEIIEFCKARMAKFKVPK
                     EVEFREALPKNMVGKMLRRLLVEEEKAKLKK"
BASE COUNT          275 a          522 c          468 g          348 t
ORIGIN      
        1 ggggatgccc gccaacaggg acgccacccg ggccacgttg ggcgcgtcgg agaactggcc
       61 cacgcagccc aggatgacct cgtcgatctc ggagggtttc aggttgttgc gcttgatgag
      121 ttccttcatg accgtggcgg ccatgtcctg cggcgtcacg tccttcaagg ccccgcccaa
      181 gttgccgatg ggcgtcctgc ccccatccac gatgaccacg tctttcatag aacacctctt
      241 tcaccacaag ccccctgcgg gggccacaaa gtccacaaag tagagcgaga tggtcctcaa
      301 aacttcgtgc acttcgtggc gccgcaggcg ctcatggtgg attatttttt cagttttgcc
      361 ttttcctctt cgaccaacag gcgcctcagc atcttgccca ccatgttctt ggggagggct
      421 tccctgaatt ccacttcctt gggcaccttg aacttggcca tgcgggcctt gcagaactcg
      481 atgatctcgg gcgcggtggc cgcttggccc ggcttcaaca cgatgtaggc cttgatcttg
      541 tccaccagct tctcgtcggg cacgcccgcc accacgcagt ccagcacctt ctcgttctgg
      601 aacaagacct cttccacgtc gcgcgggtag acgttctcgc cgcccacctt caccatgtcc
      661 ttcttgcggt ccacgatggc gaagaaaccg tcctcgtcca tgtagccgat gtcgcccgtg
      721 tagagccagc cgtcgcgcag caccatcttg gtctcttccg gatggttcca gtagccctgc
      781 atgatctggg gccccttgat gaccagctcc ccggtctcct tcacgcccat ctgctgggtg
      841 cccgtctcca cgtccatgat cttggcgtcg gtgtccggaa agggcaggcc gatcattccc
      901 agcttgcgct tgccgtagac cggattgcaa tgggtgaccg gggaggcctc ggtcaaacca
      961 taaccctcga ccagcttgcc cccggtcagg gcctcgaatt tcttctgcac ctcctggtgc
     1021 aggggcccgg cgcccgagat gcaggccttg atgctgtgga cgtcgtactt ccccacttca
     1081 ggatagttgt tgatggccac gtagatggcc tgaacgcccg ggaagatgct gaccttgtac
     1141 ttgtccacgg ccttcaagac ctgcaccacg tcgaacttgg gcaccagcac catggaagcc
     1201 gccatggaaa tggcgaaggt catgccggcc gtcatgccgt aggaatggaa gatggggatg
     1261 gccagcatga agacttcctt gccttccggc agccccggga accaggactt gcactggtag
     1321 gcgttggcca ccatgttgcg gtgggtcatg atgacgccct tggagacgcc cgtggtgccc
     1381 cccgtatatt gaagaagggc cgtttcatcc atccccaggg gcctgcgctg gggcgtggcc
     1441 ggggcctttt ccacctcgtg gtggaaggaa cggaacaggg gcccggtggg ccagtccacc
     1501 caggtctttt ccttcttggc cttgagggga tagagccact tgagggggat ggggaaataa
     1561 tcgttgatgc gggtcaccca gacccgtttc aggccgcagg cttcccccac ctc
//
LOCUS       DASTJJ010000240         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1136639, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000240 DASTJJ010000000
VERSION     DASTJJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136639"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..2234)
                     /locus_tag="VFR02_02925"
     CDS             complement(186..2234)
                     /locus_tag="VFR02_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET9869438.1"
                     /translation="MNVADRLSPVVKGFKIEDRPQQNDMARAVGETLAQGGVLLAEAG
                     TGVGKSFAYLLPALEHALSRGGPVVVSTHTLTLQEQLYRKDLPLVLKALEAEGLGPAQ
                     AVLLKGRGNYLSKRRLRLAAESHRQMELDGGSDFLKLQEWASISPEGTLSTAGFPVPA
                     DAWAEVKSDPYHCLGPRCETYETCFYHSVRRKAYQGHLILVNHALLLADLALKMDEQP
                     GVLPPYETLIVDEAHHLPALAAEHLATSVTFSECMGLVRRLLYADDKSKEGRKGLFAL
                     VPTAGAGEAVMDFKAGAERFFGLLGAGLAVGGQSRALKLPDPPPPLEMISDPLRRISN
                     LLELAESAASTEELSLEARGTREECRGLVQRLSYILDRAWDEDACYLVEPDAAALRRG
                     EIRAASVKAVPLDASGLLREHLLSSLRSVVFTSATLSVGRDFSYFRHALGLGEDLPLP
                     VATLQAGSPFNFKKQVTLFLPRSMPHPRREEEKYNRSVIDYVRASLKLSHGRAFVLFT
                     SFRMLRQVAEAIRPDLDQMGITLLVQGEDGWDRSKLLQVFREDIHSVLLGVNSFWEGV
                     DVPGEALSNLIITKLPFQVPEGPLVEARHARLREQGKEPFQVESLPEAVLRLKQGFGR
                     LIRSASDVGTVTILDPRLTSERWGKIFLGALPECETVFLAKPGTPEPARAPKPAKKRG
                     "
     gene            complement(2293..3072)
                     /locus_tag="VFR02_02930"
     CDS             complement(2293..3072)
                     /locus_tag="VFR02_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9869439.1"
                     /translation="MKPSLRAGWLAAVCSLLFLPAVFAQEPPPAAGEGGAAQYVLEDI
                     QGGVQVLETGGTQWEDGEEGQVLEAGDEIKVGPDSEATLTLGTDTSVHLDPGTDLKVE
                     QMTDNPQGGFLSRLGLAAGRILADVQKHLEESHSTFEVEAGGVVCGVRGTAFEVEADG
                     EQVQALTHEGEVDVKGAGQDNLVRAGSAFSFRRGRFVARRLLDRVETARFAKWRAFRA
                     HVFEKRRQRLEDIRRGLRRPWVRRALRRHHRQMIRERRQDR"
     gene            complement(3114..3533)
                     /locus_tag="VFR02_02935"
     CDS             complement(3114..3533)
                     /locus_tag="VFR02_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET9869440.1"
                     /translation="MIYCAFVGGFKDPRLAQLADDYARRLERLWPVTRVEIKENPKDI
                     LKYIEAKKGRADLVSLDAPGESLDSPGFARWVTHSPRDLHFFGWGASGPPAGMAGHFI
                     RSLSLSPMTFSHEMARVLLMEQLYRAGALLKGHPYPK"
     gene            complement(3530..>4619)
                     /locus_tag="VFR02_02940"
     CDS             complement(3530..>4619)
                     /locus_tag="VFR02_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869441.1"
                     /translation="MVTASHNPPEHNGFKFFPGGMPAGPEWLDGFYQAVRKGEARKGA
                     GVSEKKDFLPDYRNGLVNQAGSHFKGMRLVVDPGNGASVLTAAPVLQTLKCEIEWLNP
                     KLDGRFPGRGADSADPGGLEALAARVRKTKAQVGLAFDGDGDRLSLVDEKGRVIPNDL
                     LLCLFAQWFLPGVRRPKLVFDGRCSDWVERVALEMGCEPLLERSGHAYIFDRMRREGA
                     VLGGEASGHFFLPGGFPGDALFAGLTVLEILQEGQKPLSQYFERFPDRVTTHDLRVPL
                     EPAAVTEVYAKLKSKAEVLGAKVSTVDGVRAVFQEGWGIARRSVTEQVLSFRLEAPNR
                     RKLEELSGLWLQEFPEIQQLVLKSRLSA"
BASE COUNT          773 a         1640 c         1459 g          747 t
ORIGIN      
        1 ggaagacgtc tcagggagtt tcagtttggg cttcggcatg gattttattt tacaggcaaa
       61 cccgggggct tcaacggcca ttttcaccaa gcgcggggcc atccggggcg gaatcccggg
      121 ggaacggcaa gacgcccgcc acgacgtgac cggccttccc ttccaagccc ggcgcggccc
      181 gggggtcacc cccgcttttt ggccggcttt ggggcccggg cgggttcggg cgtgcccggt
      241 ttggccagga acaccgtctc acactcgggc agcgcgccca agaagatctt tccccaacgc
      301 tcgctggtca accggggatc caggatggtc accgtcccca cgtcgctggc gctgcggatg
      361 agccggccga aaccctgctt caaccgcagg accgcctccg gcaggctttc cacctggaag
      421 ggctccttgc cctgttcgcg caggcgcgcg tgccgggcct ccaccagggg accctcgggc
      481 acctggaagg gcaacttggt gatgatgagg ttcgacaggg cctcgccggg cacgtccacc
      541 ccttcccaga agctgttcac ccccaacaat accgaatgga tgtcctcccg gaagacctgc
      601 agcagcttgc tgcggtccca gccgtcctcg ccctggacca gcagggtgat gcccatctgg
      661 tccaggtcgg ggcggatggc ctccgccacc tggcgcagca tgcggaaact ggtgaaaagc
      721 acgaaggccc gcccatggga tagtttcaag gaggcccgca catagtcgat gacggagcgg
      781 ttgtactttt cctcctcccg gcggggatgg ggcatggacc ggggcaggaa caacgtcacc
      841 tgtttcttga agttgaaggg cgatccggcc tgcagggtgg cgaccggcag gggcaggtct
      901 tcccccaacc ccaaggcatg gcggaaataa gaaaaatccc gccccacgga caaggtggcg
      961 ctggtgaaga ccaccgacct caacgaggac aagaggtgct cccggagcag gcccgaggcg
     1021 tccagcggca cggccttgac cgaggccgcc cttatctcgc cccggcgcaa ggcggccgcg
     1081 tccggctcca ccaggtaaca ggcgtcctcg tcccaggccc ggtccaagat ataggaaaga
     1141 cgctgcacca gtccccggca ttcctcccgg gtgccccggg cctccaagga caattcctcg
     1201 gtggaagcgg cggattcggc caactccaac aggttcgaaa tgcggcgcag ggggtccgag
     1261 atcatctcca ggggcggggg cggatcgggc agcttcaaag cgcgggactg gccgcccacg
     1321 gcgagtcccg cgcccagcag gccgaaaaag cgctcggcac cggccttgaa gtccatcacc
     1381 gcctcccccg cgcccgccgt gggcaccagc gcgaagaggc ccttgcggcc ttccttcgac
     1441 ttgtcgtcgg cgtacaacag gcggcgcacc aggcccatgc attccgaaaa ggtcaccgag
     1501 gtggccaagt gctcggccgc cagggccggc aggtggtggg cctcgtccac gatgagcgtt
     1561 tcataggggg gcaagacgcc gggttgttcg tccatcttga gggccaggtc ggccagcaag
     1621 agggcgtggt tcaccaggat caggtggccc tggtaggcct tgcgccgcac cgaatgataa
     1681 aaacaggtct cgtaggtctc gcaccggggg cccaggcaat ggtaagggtc gctcttcacc
     1741 tcggcccagg cgtccgccgg gacggggaac ccggccgtcg acagggtccc ttcggggctg
     1801 atggacgccc attcctgcag cttcaggaag tccgatccgc cgtccagttc catttgccgg
     1861 tgggactcgg ccgccagccg caggcgccgt ttggaaaggt aattacccct gcccttcaag
     1921 agcacggcct gcgccgggcc caggccctcg gcctccaggg ccttgagcac caggggcagg
     1981 tccttgcggt agagctgttc ctgcagggtc agggtatggg tggacaccac caccgggccg
     2041 ccccggctca gggcgtgttc cagcgccggc agcaggtagg cgaaactttt ccccaccccg
     2101 gtgcccgcct ccgccagcag cacgccgccc tgggccaggg tctcccccac cgcgcgggcc
     2161 atgtcgttct gctggggccg gtcctcgatc ttgaaaccct tcaccaccgg tgaaaggcgg
     2221 tccgccacgt tcatgttcat tcctcatggg cgcccgaagg cgtccggttc tctcgtccca
     2281 gccttcatcg tctcagcggt cctggcgccg ctcgcggatc atctggcggt ggtgccggcg
     2341 caaggcccgg cggacccagg gacgccgcaa gccgcggcgg atgtcctcca accgctggcg
     2401 gcgtttttcg aacacatggg cccggaaggc ccgccatttg gcgaaccggg ccgtttccac
     2461 ccggtccagg agccgtcggg ccacgaaccg cccgcgccgg aacgagaagg cgcttcccgc
     2521 ccgcaccaag ttgtcctgcc ccgcgccctt cacgtccact tccccttcgt gggtcagggc
     2581 ctgcacttgc tccccgtcgg cctccacctc gaaggccgtc ccccggacgc cgcagaccac
     2641 cccgccggcc tccacctcga aggtggagtg gctctcctcc aagtgcttct gcacatccgc
     2701 caggatgcgg ccggcggcca ggcccaggcg gctcaggaac ccgccttgcg ggttgtccgt
     2761 catctgctcc accttcaagt cggtgccggg gtccaaatgg accgacgtgt ccgtccccag
     2821 ggtgagggtg gcctcgctgt ccggccccac cttgatctca tcccccgcct ccagcacttg
     2881 gccttcctcg ccgtcctccc actgggtccc gcccgtttcc agcacctgca cgccgccctg
     2941 gatgtcctcg aggacatatt gggccgcccc cccttccccc gccgcgggtg ggggttcctg
     3001 ggcgaagacc gcggggagga aaaggagcga acagaccgca gccaaccagc cggcgcgaag
     3061 ggacggtttc acggaggtac ctccaggatg ggatggggtc attttaccgg atttcacttc
     3121 ggataagggt ggcctttcaa gagcgccccc gcccggtaca actgctccat caggagcacc
     3181 cgggccattt catgggaaaa ggtcatcggc gacaggctca ggctccggat gaaatgcccg
     3241 gccatcccgg cgggcggccc ggaagcgccc cacccgaaaa aatgaaggtc ccgcgggcta
     3301 tgggtgaccc accgggcgaa gcccggcgaa tccaggcttt ccccaggggc gtccagcgac
     3361 accaggtccg cccggccttt tttcgcctcg atatatttca ggatgtcttt ggggttttcc
     3421 ttgatctcca cccgggtcac gggccaaagc cgctccaggc gccgggcata gtcgtcggcc
     3481 aattgggcga ggcgcgggtc cttgaatccc cccacgaagg cgcaatagat catgccgaca
     3541 ggcggctctt caggaccaac tgctggatct cggggaattc ctgcagccag aggccggaca
     3601 attcctccaa cttgcgccgg ttgggggctt ccagccggaa ggagagcacc tgttcggtca
     3661 ccgaccgccg ggcgatgccc cacccttcct gaaagacggc ccggacgccg tccaccgtgg
     3721 agaccttggc ccccaagacc tccgccttgc tcttgagctt ggcgtagacc tcggtgaccg
     3781 cggcgggttc cagcggtacc cgaaggtcgt gggtggtcac ccggtccggg aaccgctcaa
     3841 aatattggga gaggggtttc tgcccttcct ggaggatctc caacaccgtc agccccgcga
     3901 agagcgcgtc ccccgggaac ccgcccggca ggaaaaaatg cccgctggcc tccccgccca
     3961 acaccgcgcc ttcccggcgc atccggtcga agatataggc gtggcccgac ctctccaaaa
     4021 ggggttcgca ccccatttcc agggccaccc gttccaccca gtcggaacag cggccgtcaa
     4081 agaccagctt gggccgccgc acgccgggca ggaaccactg ggcgaacagg cacaggagca
     4141 ggtcgttggg gatgacccgg cccttctcat ccaccaggct gagccggtcc ccgtcgccgt
     4201 cgaaggccaa gcccacctgg gccttggtct tgcgcacccg ggccgccaag gcttccagtc
     4261 cgccggggtc cgcgctgtcc gccccccgtc ccgggaaacg gccgtccagt ttggggttga
     4321 gccactcgat ctcacacttg agggtctgca acaccggcgc cgccgtcaag acggacgcgc
     4381 cgttccccgg atccaccacc aaccgcatgc ccttgaaatg gctcccggct tggttcacca
     4441 acccgttgcg gtaatcgggg agaaaatcct tcttctcgct cacgcccgcc cccttgcggg
     4501 cctcgccctt ccggacggcc tgatagaaac cgtccaacca ctcgggaccc gcgggcatcc
     4561 cgcccgggaa gaacttgaag ccgttgtgct ccggcgggtt gtgggaggcg gtgaccatg
//
LOCUS       DASTJJ010000241         5410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_891905, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000241 DASTJJ010000000
VERSION     DASTJJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891905"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..3814)
                     /locus_tag="VFR02_02945"
     CDS             complement(125..3814)
                     /locus_tag="VFR02_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET9869442.1"
                     /translation="MKNIFLLLAALSGLAPATLWAQAYTWQNVVIMGGGFVAGVDYSP
                     VSQGLIYARTDVGGLYRWNNSAGQWVPLTDMYIGGQNFGGESIAPDPVNPNLVYAAVG
                     LSPSTPGTILSSINQGASWTANAIAVSMSGNGDGREAGERLAVDPNLNSKLYFGSRWQ
                     GLWVSTNSAASWSNVSSFPVKGDAGYGLSWVIFPPPTGGYGNPSGSASATIYVGVLAM
                     SSGNSNVYRSTNGGTSWTLVSGGPSNMITPHASLGTDGNLWIVYDSGGYGPGGITTGQ
                     IWKLNTSNLAWSNVTPAAPPSGSGGYGDVSVDAENAQHAVVSTLDWWSGPDKILSTVN
                     GGGSWVTIANANSGWNAGPFSAYNNNGAIYTTNCTSNNGGTGWAGNAKIDPFNSNNSI
                     YTSGGQGAGGGVWSSANINAATQPAGVTWTFTDLGIEETVPLFLNPSAAGGILFSCMG
                     DVGGMRHSNLTQSPSLGNYCNPNFSNTNMLDFAESNTNDVVRVGNSGTTTSDIAYSTN
                     NGQAWAPWGSAPPGYGTANQMESVAVAADGSRVVVSPFSGYGTAAYASSLGGAWTSCA
                     GLPSGASVASDRSSPSTFYAVAPSQWVFGGTVTVYVSTNGGASFSQVNTIPVNYNPNG
                     NGSLVVPRPVFGEPGEFWVITDNNLYRFTNAGANVTSIPNVTAPMEVGFGKAASGQTH
                     PAVFLVGTVNGTYGFYRCDDGVGTTWNLINDANHEYGSVGWVEGDETIFGRVYIGAGG
                     RGIIYGDTSAVTPTFTPTATRTATATPTRTPSPTVTATATRTPSLTPTWTLSATSTPT
                     ATLTATRTPSPTATWTLTASATATATRTGTPAATPTATPTWSPTGTPSPTLTASATPT
                     VTASPTPTFTATCTTTVLPSSTPTWTPTVTWTRTATGTPTPSATLTQTLSPTATTTVL
                     ASATPTPTSTSTSLPTITSTVTASWTPTRTATATPTATPSVTATSTPTGLPSATPTWT
                     ATATLTRTASPTPTVTFSPTFSPTPTWTSTPSATETPLPEPTAVIDFSAAPAMAVPNG
                     AVTFTIQMTVTGSAFSDYVLGLTLPTGVTFGAFTAGPAGTVVGGDIVWDIASLPPGTY
                     TYAVAAQVPGSASGVLTAQGGFGSAIGSSQAFSASVTVAPFTPTPTLVPVSYPVVYPN
                     PSTGPGPVNIRLPDFPGTADISVQVFTTAFRLVENFTAKNETAQSRVALPLTDEEGIP
                     LANGLYYVRVTTPNGTDVFKWLILR"
     gene            complement(4061..>5410)
                     /locus_tag="VFR02_02950"
     CDS             complement(4061..>5410)
                     /locus_tag="VFR02_02950"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HET9869443.1"
                     /translation="YSPGGWGYVGGTAYSSAGPISGSTDPTLYLTERAAGSFQYVFSQ
                     VPAGTYDLTLLFAETSHTAAGQRVFTVTDGNAAGTATLLSNFDIYAAAGGKNKAVDES
                     FTITQASTGPLTISFNASVDVATLQALSLVRIATPTSTPTLSPTVSPTATRTPTPTVS
                     RTPSLTPTVTLTPSPTPSWTASPTATQTPTLSPSPSPTVTFSPTMTLSATVTASRTAT
                     LSPTLTPTAVWTFTPTPTPSPSPTASPSATASPTGTATPSSSPTASTTPALTATGTAS
                     PSPTLTRTFSPTITPTPTPSPTRTPFPTQTLTPTVTALWTGTPSPTATRTATWSPTPT
                     ASSTPTATRTPPGSPTPTVAASPAATPVLYPNPVSGSGPARLRLPDYQGFADVPVKIY
                     TTAFRMVESFTAPRQAGGATVLVPLTDKSGAPLADGLYYVAVTTPDGRSILKMLLLR"
BASE COUNT          917 a         1535 c         2030 g          928 t
ORIGIN      
        1 caggaggcct caaagccctt ggctttgcgg cgatccaagg cgcgctgagc ctggtgggct
       61 cagcgcagga ggcctcaaag cccttggctt tgcggcgatc cacgacgcgc tgagcctggt
      121 gggctcagcg caggatgagc catttgaaga cgtccgtgcc gttgggcgtg gtgaccctta
      181 cgtaataaag cccgttggcc aaggggatcc cctcttcatc cgtcaggggc agcgccacac
      241 ggctttgcgc cgtctcgttc ttcgccgtga aattttccac caaccggaaa gccgtagtga
      301 agacctgcac cgaaatatcc gccgtccccg ggaaatccgg caagcggatg ttcaccgggc
      361 ccgggcccgt ggagggattc ggatagacca ccggatagga aaccggaacg agcgtggggg
      421 tgggcgtgaa gggcgccacc gtcaccgagg cggaaaaggc ctgggaactc ccgatcgccg
      481 aaccgaaccc gccttgggcc gtcagcacgc ccgaggccga accggggacc tgggccgcca
      541 ccgcgtaggt gtaggtgccc ggggggagcg acgcgatgtc ccacacgatg tcccccccca
      601 cgaccgttcc cgcgggcccc gccgtaaagg cgccaaaagt gacgccggtg ggcagggtca
      661 agcccaacac ataatccgaa aaagccgacc cggtcaccgt catttggatg gtgaaggtca
      721 cggccccatt gggcacggcc atcgccgggg cggcgctgaa gtcgatcacc gccgtgggtt
      781 ccggcagcgg cgtttcggtc gcggaaggcg tggaggtcca ggtcggggtc ggactgaagg
      841 tgggggaaaa ggtcaccgtc ggcgtggggg acgccgttcg ggtgagggtg gccgtcgcgg
      901 tccaggtcgg cgtggccgaa ggcaagccgg tcggggtgga ggtcgccgta acgctggggg
      961 tggcggtcgg cgtggcggtc gccgtccggg tgggggtcca actggcggtc acggtggagg
     1021 tgatggtcgg caaagatgtg gaggtggacg tcggggtcgg cgtggccgag gccagcaccg
     1081 tcgtggtggc ggtggggctc aaggtctggg tcagggtggc gctcggggtc ggggtcccgg
     1141 tggccgtccg ggtccaggtg acggtcggcg tccaggtggg ggtgctggaa ggcaggacgg
     1201 tcgtggtgca ggtggcggtg aaggtcgggg tcggggaggc ggtcaccgtc ggcgtggcgc
     1261 ttgcggtcaa ggtcgggctg ggcgtccccg tcggggacca ggtcggggtg gcggtgggcg
     1321 tcgcggcggg ggttcccgtc cgggtcgccg tcgccgtggc ggaagccgtc aaggtccagg
     1381 tcgccgtggg ggacggcgtc cgggtcgcgg tcagggtggc ggtgggcgtg gaggtcgccg
     1441 agagggtcca agtcggggtc aggctgggcg tacgggtggc ggtggccgtc accgtcgggg
     1501 acggggtgcg ggtgggcgtc gccgtggccg ttcgggtggc cgtgggggtg aaggtgggcg
     1561 tcacggcgct ggtgtcgccg taaataatgc cccgtccgcc ggcgccaata tagacgcgtc
     1621 caaagatcgt ctcatcccct tccacccatc ccacgctccc gtactcgtgg ttggcgtcat
     1681 tgatcaggtt ccaggtggtg ccgaccccgt cgtcacagcg ataaaacccg taggtcccat
     1741 tcacggtccc gaccagaaaa accgccgggt gggtctggcc ggaagcggcc ttgccgaatc
     1801 ccacctccat cggggcggtt acattgggga tcgaagtcac gttggcgccc gcgttggtga
     1861 accggtagag gttgttgtcg gtgatgaccc agaattcccc gggctctccg aaaacgggcc
     1921 ggggaacgac cagcgatcca ttgccgttgg ggttgtaatt caccggtatg gtgttcacct
     1981 gggagaaact ggctccccca ttggtgctca cgtaaaccgt cacggtgccg ccaaagaccc
     2041 actggctcgg ggcaacggca tagaaggtgg aggggctcga ccggtcggaa gccacagagg
     2101 ctccgctggg aaggcccgcg cagctggtcc aggcgccgcc cagggaagaa gcgtaggcgg
     2161 ccgtcccata accgctaaag ggcgacacga ccacccggga accgtccgcg gccacggcca
     2221 cggattccat ctggttcgcc gtgccgtaac ccgggggcgc gcttccccag ggcgcccagg
     2281 cttgcccgtt gttggtggaa taggcgatgt cggaagtggt cgtcccgctg ttgcctaccc
     2341 gcaccacgtc gttggtattg ctttcggcaa agtccaacat gttcgtgttg ctgaagttgg
     2401 ggttgcaata attgcccagg gagggcgact gggtcaagtt cgagtggcgc ataccgccca
     2461 cgtcgcccat gcaggaaaag aggatccccc ccgccgcgga aggattcaag aataggggca
     2521 cggtctcctc tattcccagg tcggtaaagg tccatgtgac gcccgcgggt tgggtcgcgg
     2581 cgttgatatt ggcgctgctc cagaccccgc cgccggcccc ttgccccccc gacgtgtaaa
     2641 tggagttgtt ggaattaaaa ggatcgatct tggcgtttcc cgcccaaccc gttcctccgt
     2701 tgttggaggt gcaattcgtg gtatagatgg cgccgttgtt gttgtaggcc gaaaaaggcc
     2761 ccgcgttcca accactgttg gcgttggcaa tggtcaccca cgaaccgccg ccgttgacgg
     2821 tcgagagtat tttgtccggc ccgctccacc aatcgagggt ggagacaacg gcatgctggg
     2881 cattttcggc gtccacggaa acgtcaccat agccgccgct gccgctgggg ggggcggcag
     2941 gggtcacgtt ggaccaggcg aggttcgagg tgttcagttt ccagatctgg ccggtggtaa
     3001 tgccgcccgg gccgtaaccg ccgctgtcgt aaacgatcca gagattccca tcggttccca
     3061 ggctggcgtg gggggtgatc atgttggaag gaccgcccga aactaaagtc cagcttgtgc
     3121 ccccgttggt tgaacggtag acgttgctgt tgcccgagct catcgccaag acacccacat
     3181 aaatggtggc ggaggcggag cctgagggat taccgtagcc accggtgggc gggggaaaga
     3241 tgacccagga aagcccatat ccggcgtccc ctttcactgg aaaagaggat acgttgctcc
     3301 aactggccgc cgagttggtg cttacccaaa gcccctgcca gcgggagccg aagtaaagtt
     3361 tggaattaag attggggtcg accgccaggc gttcgccggc ctcgcggccg tcaccattcc
     3421 ccgacatgga aacagcaatg gcgttggcgg tccaggaagc gccctgattt atggaactga
     3481 ggatggtgcc gggggtggat gggctcaagc ccaccgccgc gtagaccagg ttggggttca
     3541 ccgggtccgg cgcgatgctt tctcccccga agttctggcc gccgatgtac atgtcagtca
     3601 gagggaccca ttggccggcg gagttgttcc aacggtataa accgccgacg tccgtacggg
     3661 cgtagatcag tccctgcgaa acggggctat agtcaacccc ggcgacgaag ccccctccca
     3721 tgatgaccac gttctgccag gtgtaggcct gggcccaaag ggtcgcgggg gccagcccgg
     3781 agagggcggc caacaatagg aagatatttt tcaattgccg caaggccata gggtcgtcaa
     3841 accatcggtc ttttattttt gaaccgggga cactggggac ggttaaaagc ttatcgctgt
     3901 tttggtcctg gtgggcctga aatcgattta tatccgggat taagacggat ctgattgaac
     3961 cgattcaacc tgatcttggc gcaccccaag attagtccca ccaagacggc cccttattcc
     4021 atcaaaaaga gccaccgggg acggacaaaa agaaggggtt tcagcgcagc agaagcattt
     4081 ttaggatgga ccgtccatcc ggcgtggtca cggccacgta atagaggccg tcggccaagg
     4141 gggcgccgct tttatccgtc aagggcacca ggaccgtggc gccccccgct tgccgggggg
     4201 ccgtaaagct ttccaccatc cggaaagccg tggtgtagat cttcacgggc acatcggcaa
     4261 aaccctggta atcgggcagg cgcaggcgcg ccgggccgga cccggagacc ggattggggt
     4321 aaagcaccgg ggtggcggcc ggggaagccg ccacggtggg cgtgggggag ccaggcggcg
     4381 tccgggtggc ggttggggtg gaggaagccg tgggggtcgg gctccaggtc gcggtccggg
     4441 tcgccgtggg gctgggggtg ccggtccaaa gggccgtcac ggtcggcgtc agcgtctgcg
     4501 tcgggaaagg cgtccgggtg gggcttgggg tcggcgtggg cgtgatcgtc ggggaaaagg
     4561 tccgggtcaa agtgggtgag ggcgaagccg tccccgtggc ggtcaaggcg ggcgtcgtcg
     4621 aggccgtggg actggacgaa ggggtcgccg tgccggtggg actggccgtc gcggacggcg
     4681 acgccgtggg ggagggcgac ggggtcggcg tcggggtgaa ggtccaaacc gcggtcgggg
     4741 tcaaggtggg ggacagggtc gccgtgcggg acgccgtcac cgtcgccgac aaggtcatgg
     4801 tcgggctgaa ggtcacggtg ggtgacgggg acggggaaag cgtgggggtt tgggtggccg
     4861 tgggggaagc ggtccaggaa ggcgtggggc tgggcgtcag ggtcaccgtg ggcgtgaggc
     4921 tgggggtacg gctcacggtg ggcgtggggg tccgggtcgc cgtgggggac accgtgggcg
     4981 acagggtcgg ggtcgaggtg ggcgtggcga tgcgcaccaa ggagagcgcc tgcagggtgg
     5041 ccacgtccac cgaggcgttg aaggagatgg tcaagggccc ggtggaagcc tgggtgatgg
     5101 tgaagctttc gtcgaccgcc ttgttcttgc cccctgccgc cgcgtagatg tcgaagttgg
     5161 ataaaagcgt ggccgtgccc gcggcgttcc cgtcggtcac ggtgaagacc cgttgacccg
     5221 ccgccgtatg ggaggtctcg gcgaacaaca aggtcaggtc gtaggtgccc gccggcacct
     5281 ggctgaagac gtattggaag gatcccgccg cccgctcggt caggtagagg gtggggtccg
     5341 tcgagcccga aatggggccc gcgctgctgt aggccgtgcc gcccacgtag ccccaaccgc
     5401 ccgggctgta
//
LOCUS       DASTJJ010000242         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1381111, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000242 DASTJJ010000000
VERSION     DASTJJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381111"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFR02_02955"
     CDS             <1..920
                     /locus_tag="VFR02_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9869444.1"
                     /translation="GRFGLRNGAGRLLLDLADAYYSVLELGDTLKTDQDSRDLTQKIL
                     DVQKHWVAIGRAQKSDMLSTSAQLAQLDATLQSDRTQLTQEREELAFLAGVPPDVALE
                     VPDTGASLPAYGLEQALAKVDARPDVAQAKAQWDVAEAQVLAAHGQHLPTLVASGNYA
                     LAADGANSPREWTVGLQASIPVFEGGQLVAQERQADSKRNQAKLAWSQARRQALEDIR
                     KAYQGLQDSLQQTQAFQASQQASQEAYEAVLHDYNLNLQTPLALLQALNTLEAAKADY
                     AKTRYQTLYDQVWLGVALGDLPQVTGERP"
     gene            917..>2029
                     /locus_tag="VFR02_02960"
     CDS             917..>2029
                     /locus_tag="VFR02_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9869445.1"
                     /translation="MTFSDISIRNPVMAWMAMAAMILFGIICFTRMGISQLPDVNLPV
                     VNVSITLPNAAPEVVESDVADPVESAVMGVEGVQDVQTTCTQGQASISVFLNPNRDVD
                     AAVQDVQTLVFQAEKHLPTNIFPPVIRKMNPNASPILWMAVTADPPLTTKDLMLYTRD
                     HIQDRFTSLDGVSNVFLGGFRDREINIWADNQKLNARQLTVNDVINAIQHQHAEVPGG
                     SMETPDTQYDIRTMGEAYQVDEFARLPILARGGSPNYSATRIGDVATIEDGVASPVTR
                     LSRFDGTTAVGLGIVMQDGYNAVAVGDAVKARMAEVQKRLPPGYHITLNFDTTTAIRD
                     NVHELELTILLAVGLTSLVCFLFLGSLTSTLNVFLAI"
BASE COUNT          366 a          754 c          616 g          293 t
ORIGIN      
        1 cgggccgctt cggcctgcgc aacggcgccg gccgcctgct gctggacctg gcggacgcct
       61 attattcggt gctggagctg ggcgacaccc tgaagaccga ccaggattcc agggacctga
      121 cccaaaagat cctggacgtg cagaagcact gggtggccat cggccgcgcc cagaagagcg
      181 acatgctgag cacctccgcc caattggccc aactggacgc caccctgcag agcgaccgca
      241 cccaattgac ccaggagcgg gaggagttgg ccttcctggc gggcgtgccc ccggacgtgg
      301 cgctggaggt ccccgatacc ggcgcttccc tgccggccta cggcctggaa caggccctgg
      361 ccaaggtcga tgcccggccc gacgtggccc aggccaaggc ccagtgggac gtggcggaag
      421 cccaggtctt ggcggcccac ggccagcacc tgcccaccct ggtggcgagc ggcaactacg
      481 ccctggcggc cgacggggcc aattcgcccc gggaatggac cgtggggctc caagcctcga
      541 tcccggtctt cgagggcggc caactggtgg cccaggaacg gcaggccgac tccaagcgca
      601 accaggccaa gctggcctgg tcccaggccc gccgccaggc cctcgaggac atccgcaagg
      661 cctaccaggg cctgcaggat tccctccaac agacccaggc gttccaggcc tcccagcaag
      721 cctcccagga agcctatgag gccgtgctgc acgactacaa cctgaacctg cagacgcccc
      781 tggccctgct gcaggccctc aacaccctgg aagccgccaa agccgattac gccaagaccc
      841 gttaccagac cctctatgac caggtctggc tgggcgtggc cctgggcgac ctgccccaag
      901 tgaccgggga gcgcccgtga ccttctccga catctccatc cgcaacccgg tcatggcctg
      961 gatggccatg gcggctatga tcctgttcgg gatcatctgc ttcacccgca tgggcatttc
     1021 ccagctgccc gacgtgaacc tgcccgtggt gaacgtctcc atcacgctgc ccaacgcggc
     1081 ccccgaggtg gtggagtccg acgtggcgga cccggtggaa tcggcggtca tgggcgtgga
     1141 aggcgtgcag gacgtgcaga ccacctgcac ccagggccag gccagcatct cggtgttcct
     1201 caaccccaac cgcgacgtgg acgccgcggt gcaggacgtg cagaccctgg tcttccaggc
     1261 cgagaagcac ctgcccacca acatcttccc gccggtcatc cgcaagatga accccaacgc
     1321 ctcccccatc ctctggatgg cggtgaccgc cgacccgccc ttgaccacca aggacctgat
     1381 gctctatacc cgcgaccaca tccaggaccg cttcaccagc ctggacggcg tgtccaacgt
     1441 ctttttgggc ggcttccggg accgggagat caacatctgg gccgacaacc aaaagctcaa
     1501 cgcgcgccag ctgaccgtca acgacgtcat caacgccatc caacaccagc acgccgaggt
     1561 gccgggcggg tccatggaga cccccgacac ccagtacgac atccgcacca tgggcgaggc
     1621 ctaccaggtg gacgagttcg cccgcctgcc catcctggcg cgcggcggct cgcccaacta
     1681 cagcgccacc cgcatcggcg acgtggccac catcgaggac ggcgtggcca gcccggtcac
     1741 ccgcctgtcg cgcttcgacg gcaccacggc ggtgggcctg gggatcgtca tgcaggacgg
     1801 ctacaacgcc gtggcggtgg gcgacgcggt caaggcccgc atggccgagg tccaaaaacg
     1861 gctgccgccg ggctaccaca tcaccctcaa cttcgacacc accaccgcca tccgggacaa
     1921 cgtgcatgag ctggagctga ccatcctctt ggccgtggga ctgacctcgc tggtctgctt
     1981 tctgttcctg ggcagcctga cctcgaccct gaacgtcttt ttggccatc
//
LOCUS       DASTJJ010000243         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_524810, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000243 DASTJJ010000000
VERSION     DASTJJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524810"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="VFR02_02965"
     CDS             complement(<1..>1079)
                     /locus_tag="VFR02_02965"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9869446.1"
                     /translation="LHVFLPPLSLLEHFLELVGRVEAAASALHLSVVVEGYEPPADPR
                     LLNFRVTPDPGVLEVNVHPAADWPSLKTEMEVLYEEARKTGLSAVKFTVNGRSVGSGG
                     GSHLVLGASIPEQSPFFRQPRLLPAMISYWQRHPSLSYFFASQSVGPTSQAPRPDEAR
                     HDALYELENAMRRIPKEGALPFWRLDRLFRHLLVDVSGNTHRTEFCIDKLFDPSSTRG
                     RQGLLELRAFEMAPHPRMALLQALLVRALVAKLYTAPLPDQLTRWGTALNDRFMLPWY
                     LWQDLTEVLEDLSRDGLAFDPGWFQAHFNFRFPLCGKALIEGFELEVRTALEPWPVLG
                     EEAGPAVSRAIDASTERVQVSLKDAP"
BASE COUNT          193 a          349 c          389 g          148 t
ORIGIN      
        1 ggcgcgtcct tcagcgacac ctgcacccgc tcggtggaag cgtcgatggc ccggctcacg
       61 gcggggcccg cctcctcccc cagcacgggc cagggttcca gggcggtgcg cacctccagc
      121 tcgaacccct cgatgagcgc cttgccgcaa agcgggaagc ggaaattgaa atgggcctgg
      181 aaccagcccg ggtcgaaggc caggccgtcc cgcgacaggt cctccaacac ttccgtcaag
      241 tcctgccaca aataccaggg aagcatgaac cggtcgttca aggccgtgcc ccagcgcgtg
      301 agctggtccg gcaggggggc ggtatagagc ttggccacca gggcccgcac caggagggcc
      361 tgcagcaggg ccatgcgggg atggggggcc atctcgaagg cgcgcagttc caacaggccc
      421 tgccgccccc gggtggagga ggggtcgaaa agcttgtcga tgcagaactc ggtgcggtgg
      481 gtgttgccgc tcacatccac cagcaggtgg cggaagagac ggtccaggcg ccagaagggg
      541 agcgcgcctt ctttggggat gcggcgcatg gcgttctcca attcgtaaag cgcgtcgtgg
      601 cgggcctcgt cgggccgggg cgcctggctg gtggggccca cggactggga cgcgaagaaa
      661 taggacaggc tggggtgccg ctgccagtag gagatcatgg cgggcaagag ccggggttgg
      721 cggaagaagg ggctctgttc ggggatggag gcgcccaaca ccaggtggct cccgccgccc
      781 gagcccacgg accggccgtt gacggtgaac ttgaccgccg aaagcccggt cttccgggcc
      841 tcctcgtaaa gcacctccat ctcggtcttg agcgagggcc aatccgccgc gggatgcacg
      901 ttgacctcca gcacgcctgg gtccggcgtg acgcggaaat tgagcaggcg cgggtcggcg
      961 ggcggctcat agccctccac caccacgctc aagtggagcg cgctcgcggc cgcttccacc
     1021 cgtcccacca gctccaggaa atgctccaac agggaaagcg gcggcaggaa gacgtgcag
//
LOCUS       DASTJJ010000244         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_403221, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000244 DASTJJ010000000
VERSION     DASTJJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403221"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFR02_02970"
     CDS             complement(<1..443)
                     /locus_tag="VFR02_02970"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET9869447.1"
                     /translation="MTFRLFALACLAFATAVPGLAETDSTQPHVSVEMIGADSTLQPG
                     RPVTVGFHFKIDRGWHLYWQNPGDSGLAPEIIWDLPPGFTAGPVLWPTPQRLALPSLV
                     DYGYRNDLLLMVPIQVPADAHPGAVIDLSAMVRWLVCDEICIPGRV"
     gene            complement(558..>1043)
                     /locus_tag="VFR02_02975"
     CDS             complement(558..>1043)
                     /locus_tag="VFR02_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869448.1"
                     /translation="GQADDALSLLKKAQELDPLQPQVQSLLEKVRRQVSHPPDEGLTE
                     DSSVGADVEADFQHSPDHTTKKTKWTEGESLSSYFKASETVKTPPHKKNVHKKQTAPH
                     REQLAAEADQTYNLGLESYRAGNYAQAQQYWQQTLDLDPTHFRAKRDLARLKAEHPGL
                     P"
BASE COUNT          176 a          287 c          373 g          207 t
ORIGIN      
        1 acccggcccg ggatgcagat ctcgtcgcaa accaaccagc gcaccatggc cgagaggtcg
       61 atgaccgccc ccgggtgggc gtccgccggc acctggatgg ggaccatcag aagcaggtcg
      121 ttgcggtagc cgtagtccac cagcgagggc agggccaggc gctggggcgt gggccaaagc
      181 acgggccccg ccgtgaagcc ggggggcagg tcccagatga tctcgggggc caggcccgag
      241 tcgcccgggt tctgccagta gaggtgccag ccccggtcga tcttgaaatg gaaacccacg
      301 gtgaccggcc gcccgggctg cagggtggaa tcggcgccga tcatctccac gctcacgtgg
      361 ggttgggtgg aatcagtctc ggccaggccg gggacggcgg ttgcgaaggc caaacaggcc
      421 aaagcgaaaa ggcggaaggt catgcggtct ccagggatct cttggtaggt ccctactata
      481 gacgttcagg aagtggattt ggatggggaa agacggtggg ccagcaatgg cttgaaggtg
      541 aaacttgaca gaccgtctca gggcagcccg ggatgctcgg ccttcaaacg cgccaagtcc
      601 cgcttggccc ggaaatgggt ggggtccagg tccagggtct gttgccagta ttgctgcgct
      661 tgggcgtagt tcccggcgcg gtagctttcc aggcccaggt tgtaggtctg gtcggcttct
      721 gcggcgagtt gctcccggtg cggcgcggtt tgttttttat ggacgttctt tttgtggggc
      781 ggggttttga cggtttcgga ggccttgaaa taagaagaga gactttcccc ttcggtccat
      841 ttggtctttt tggtcgtgtg gtccggactg tgttgaaagt ccgcttccac gtcggcgccg
      901 acggacgaat cctccgtgag cccctcgtcc ggagggtggg aaacctggcg acggaccttt
      961 tctaacaggg attgtacctg cggctggagc ggatccagtt cctgggcctt tttcaagagc
     1021 gagagcgcgt cgtcggcctg gcc
//
LOCUS       DASTJJ010000245         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1137205, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000245 DASTJJ010000000
VERSION     DASTJJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137205"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VFR02_02980"
     CDS             complement(<1..1435)
                     /locus_tag="VFR02_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869449.1"
                     /translation="MFFSTRSPKGLGHRAALGGLVACHLAVWGGVFFKAGAVWSSPLG
                     DLDRHFMAWRIFAADQLRQGILPLWTPATLNGTPFLGGFESALLYPFNWLFTVLPPAP
                     AANLVMAAQVLLAGLFTYGWAFSRGLRPAACWLAAMVASLGGPLLLQVYAGHLSNLCA
                     MAWIPFLFWAFDEFTRRPSVPWGLGLALGSCLQGLAGHPQYVYETAILLGLYAVLSGA
                     FSPSRRGRTFLAVAAYAAGFLLSAAQWLPGLQALEGSARGGLWDPKTSGLFSLPPENL
                     LTLFLPDLFGPVGSNLYWGRWYLWDVSLFMGFTPLLLAAYALSRRGGFRGPDPWTALA
                     AFLLALGPATPLFQVLFHFLPGFHSLRGTCKFDFFITLFLGLLAGSGLDRLWKQGRCP
                     KGTAWGLGLTALAAAGLGLALRASAAQGPAGAWEKGWAGLPWLQKGLAYLDPVQRDLF
                     ARGAGARAAESIFLLALTAAVLAFAEAW"
     gene            1534..2406
                     /gene="folK"
                     /locus_tag="VFR02_02985"
     CDS             1534..2406
                     /gene="folK"
                     /locus_tag="VFR02_02985"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9869450.1"
                     /translation="MPDKDYITLQGLKIKCIIGIFDWERKRKQEVRLDLKFPCDVRRA
                     SRWDDIHHTLDYKRIAKTAIGFVSESRFQLVETLAEKLAELLLERFALEELWLSVQKP
                     GAIRGSRTVGVELHRTRESAGGGVVFSLGSNIRPAENLAFALRELDRRFGLTALSRVY
                     ETSPVKLKRQPAFWNLAAAVRTRETPGRLKAWLRGLEKKAGRSRGGDPNGPRTLDVDL
                     ILWNGKVLGPSLPHPDIPHKAFVLFPLAEIFPTERHPKLGRSYLELAADFRDKHQKIR
                     LLPPGVLAWGSDRA"
     gene            complement(2409..>2608)
                     /locus_tag="VFR02_02990"
     CDS             complement(2409..>2608)
                     /locus_tag="VFR02_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9869451.1"
                     /translation="RVNAVSPGVISFPAHYGPAKRRRLAAKAALKRGGDPQDIARAVL
                     FLAREPFLTGQVLRVDGGRFL"
BASE COUNT          533 a          874 c          853 g          348 t
ORIGIN      
        1 cccaggcctc ggcgaaagcc agcaccgcgg ccgttagggc caacaggaaa atgctttcgg
       61 cggcccgggc gccggcgccg cgggcgaaaa ggtcacgttg gaccgggtcc aaataggcca
      121 atcccttttg aagccagggc agtcccgccc accccttttc ccaggccccc gccggtccct
      181 gggccgccga ggcccgcagg gccagtccca agcccgccgc ggccaaggcc gtcagcccca
      241 gcccccaggc cgtgcctttc gggcaacggc cttgcttcca taaacggtcc agcccggacc
      301 ccgccaggag ccccaggaag agcgtgatga aaaaatcgaa cttgcaggtc ccccggaggc
      361 tgtggaatcc cggcaggaaa tggaacaaga cttggaaaag cggcgtggcg ggccccaagg
      421 ccaaaagaaa ggccgccaag gccgtccaag gatcggggcc ccggaaacct ccccgccggg
      481 aaagggcgta ggcggccaac agcagcggag tgaatcccat gaagaggctg acatcccaca
      541 ggtaccaacg cccccaatag aggttgctgc ccacgggccc gaagagatcc ggcaaaaaaa
      601 gcgtgagcag gttctccggc ggaagggaaa aaagtccgga agtcttgggg tcccataatc
      661 ccccgcgggc cgacccttcc agggcctgca gcccgggcag ccattgggcc gccgacagta
      721 aaaatccggc ggcataggcc gctacggcca ggaacgtccg gccgcggcgg gagggggaaa
      781 aggcccccga caggacggcg tagagcccca gcaggatcgc cgtctcatag acatattgag
      841 ggtgcccggc caacccctgc aggcaggatc ccagggccag gcccaggccc cagggcacgg
      901 aaggccgccg cgtgaattca tcgaaggccc agaacaaaaa ggggatccag gccatggcgc
      961 agaggttgga caggtgcccg gcgtaaacct gcagcagcag gggccctccc aaggaggcga
     1021 ccatcgccgc cagccaacag gccgccgggc gcaaaccccg ggaaaatgcc catccataag
     1081 tgaagagccc cgccaagagg acctgggccg ccatgaccag gttggccgcc ggagccggcg
     1141 gcaggaccgt gaacaaccag ttgaagggat aaagaagggc ggactcgaac ccgcccaaga
     1201 agggcgttcc gttcagggtg gcgggggtcc agagcggcag gatgccctgg cgcagttggt
     1261 cggcggcgaa gatccgccag gccatgaaat gccggtccag gtcgcccagc gggctggacc
     1321 aaacggcccc ggccttgaaa aaaacaccgc cccagaccgc caagtggcag gccaccaggc
     1381 cccccagggc cgcccggtgt cccagtccct tcggggaccg cgtggaaaag aacaagccgt
     1441 ccaccttcct gggccaaaac gggcttcaat tggacccggg tttctgttga aatggggcgg
     1501 cctatcaacc ccgcggggat gaggagaacc ggtttgcccg acaaggatta tatcaccctg
     1561 cagggcctca aaattaagtg catcatcggc atcttcgatt gggagcgcaa gcgcaagcag
     1621 gaggtgcggt tggacttgaa gttcccctgc gacgtgcgcc gggcctcccg gtgggacgac
     1681 atccaccaca ccctggacta taagcgcatc gccaagaccg ccatcggttt cgtttccgaa
     1741 agccgcttcc agttggtgga gaccttggcg gaaaaactgg ccgaactgct gttggaacgt
     1801 ttcgccctgg aggagctttg gctttccgtg cagaagcccg gggccatccg gggttcgcgc
     1861 acggtggggg tggaactgca ccggacccgg gaaagcgccg ggggcggggt ggtcttcagc
     1921 ctgggatcca atatccgccc ggcggagaat ttagctttcg ccctgcggga attggaccgg
     1981 cgtttcggcc tgaccgccct gtcccgcgtc tatgagacct ccccggtgaa gttaaagcgc
     2041 caacccgcct tttggaacct ggcggccgcg gtgcgcaccc gggaaacccc cgggcgcctc
     2101 aaggcctggc tgcgggggct ggagaaaaaa gcgggccggt cgcgcggcgg ggaccccaac
     2161 ggcccccgca ccctggacgt ggacctgatc ctttggaacg gcaaggtcct ggggccttcg
     2221 cttccccacc cggacatccc ccacaaggcc tttgtgctct ttcccctggc cgagatcttc
     2281 cccaccgaac ggcacccgaa gttgggccgc agctacctgg aactggcggc cgatttccgg
     2341 gacaagcacc agaagatccg gctgctgccg cccggggtcc ttgcttgggg atccgaccgg
     2401 gcctgaattc agaggaaccg gcccccgtcc acccggagga cttgaccggt caaaaagggc
     2461 tcccgggcca ggaacaacac cgcccgcgcg atgtcctgcg ggtccccccc tcttttcagg
     2521 gccgccttgg cggccagccg gcggcgcttg gccggcccat aatgggccgg gaaggaaatg
     2581 acccccgggg acaccgcgtt gaccctga
//
LOCUS       DASTJJ010000246         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1014947, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000246 DASTJJ010000000
VERSION     DASTJJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014947"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFR02_02995"
     CDS             <1..269
                     /locus_tag="VFR02_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET9869452.1"
                     /translation="ANVEETSVGRVKVGQPVRVTVDEGGRLTGRVEEITAAAESQFSI
                     IPMENPSGNFIKLVQRIPIRVALDDPSRLPLKAGESVEVRIRVR"
     gene            290..1870
                     /locus_tag="VFR02_03000"
     CDS             290..1870
                     /locus_tag="VFR02_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET9869453.1"
                     /translation="MGSRAPAAAPGRERPNYKWEVLWVVMLGTLMAALDQSIVNIALP
                     DMMSDFGCTTEDIEWVVTGYMLSFSTLMPLTSWLRDRVGYRNIYLASLVVFTLGSVLC
                     GLAWNLPSLVFFRVVQAIGGGAITPTSLAMISEVFPPEERGQALGYWGVGVIMGPAFG
                     PTLGGFLTHTVGWRSIFWVNLPIGILGTLLALSLLRRDAPHPDQRKPFDFFGFGFLSL
                     FLVSFLLAVSQGEEKGWSSAYITVCFILSALGLVGFLVVDSLVEHGIIDFKLFRYPMF
                     AAGSLVIGARSVALFGGMFLAPIFMQNVMGFDEVQSGLIMLPGALLIAVFMPISGRIS
                     DKIGPFYPTLAGLIGVVAFMALYRGLDADTTVWGLVYPTLIRGVGIGLLIAPVMAASL
                     NVIPRASAGMASAVMNLLQQVAGSIGIAVLGTFLDHRTHFHQMALAGRITELTPAQSQ
                     VVRDLADQARLLGLNHAQAHQVGLSALAQQVATAGAVQGFDDAFLFGAAIVALGILPA
                     FLLPKHKVGAAKAPAALE"
     gene            complement(2286..>2602)
                     /locus_tag="VFR02_03005"
     CDS             complement(2286..>2602)
                     /locus_tag="VFR02_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869454.1"
                     /translation="FSKSQDLAYAHLRQYISPLSMGYSQWAQSVHGMAFKFADTLGMA
                     TPDSLVAKEAFFRVKKQAFVLGFDHMCWLMAFLFVVTIIPIYYLKKPAGWGAPGGGGA
                     GH"
BASE COUNT          378 a          893 c          829 g          502 t
ORIGIN      
        1 cggccaacgt ggaggagacc agcgtgggcc gggtgaaggt gggccaaccc gtgcgcgtca
       61 ccgtggacga ggggggccgg ctcaccggcc gggtggagga gatcacggcc gccgccgaat
      121 cgcagttctc catcatcccc atggagaacc cctcgggcaa cttcatcaag ctggtccagc
      181 gcatccccat ccgggtggcc cttgacgacc cttcccgcct gcccctgaag gccggcgaat
      241 cggtcgaggt gcgcatccgg gtgcgctgac gccccccgcg gcgggcccgg tgggctcccg
      301 cgccccggcc gccgccccag ggcgggagcg gcccaactat aaatgggaag tgctctgggt
      361 ggtcatgctg ggcaccctca tggccgcgtt ggaccaaagc atcgtcaaca tcgccctgcc
      421 cgacatgatg tcggacttcg gctgcaccac cgaggacatc gaatgggtgg tgaccggcta
      481 catgttgagt ttttccaccc tcatgcccct cacgtcctgg ctgcgggacc gcgtgggcta
      541 ccgcaacatc tacctggcca gcctggtggt cttcaccctg gggtcggtgc tttgcggcct
      601 ggcctggaac ctgccctccc tggtcttttt ccgggtggtc caggccatcg ggggcggggc
      661 catcacgccc accagcctgg ccatgatctc ggaggtcttc ccccccgagg aacgggggca
      721 ggccctgggc tattggggcg tgggcgtcat catgggcccg gccttcggcc ccaccctggg
      781 cggcttcctc acccacacgg tgggctggcg gtccatcttc tgggtgaacc tgcccatcgg
      841 catcctgggg accctgttgg ccctgtccct gctgcgccgc gacgcgcccc atcccgacca
      901 gcgcaagccc ttcgactttt tcggcttcgg ctttttgtcc ctcttcctgg tgtcgttcct
      961 tttggcggtc tcgcaaggcg aggaaaaggg ctggtcgtcg gcctacatca cggtctgctt
     1021 catcctgtcg gccctggggc tcgtcgggtt cctggtggtg gacagcctgg tggagcacgg
     1081 catcatcgac ttcaagctgt tccgctaccc catgttcgcc gccgggtcgc tggtcatcgg
     1141 ggcccggtcc gtggccctct tcggcggcat gttcctggcg cccatcttca tgcagaacgt
     1201 catgggcttc gacgaggtcc aaagcgggct catcatgctg ccgggggcgc tcctcatcgc
     1261 cgtcttcatg cccatctcgg gcaggatcag cgacaagatc ggcccctttt accccaccct
     1321 ggcgggattg atcggcgtgg tggccttcat ggccctctac cggggcctgg acgccgacac
     1381 cacggtctgg gggctggtct accctaccct catccggggc gtgggcatcg gcctcttgat
     1441 cgcccccgtc atggcggcgt ccttgaacgt catcccccgg gccagcgcgg gcatggcctc
     1501 cgcggtcatg aacctgctgc agcaggtcgc cgggtccatc ggcatcgccg tgctgggcac
     1561 cttcttggac caccgcaccc atttccacca gatggccctg gcgggccgga tcaccgagct
     1621 cacaccggcc caatcccagg tggtgcggga cctggccgac caggcccggc tcttgggctt
     1681 gaaccacgcc caggcgcacc aagtgggcct gtcggccctg gcccaacagg tggccaccgc
     1741 cggggcggtc cagggcttcg acgacgcctt cctcttcggc gccgccatcg tggccctcgg
     1801 catcttgccg gccttcctcc tgcccaaaca caaggtgggc gccgcgaagg cgccggccgc
     1861 cctggaatga aaaaggcgga cccggtcggg tccgccttct gacgggacgg gggcggcctg
     1921 aggccgcccc ttctctctct tctccacgcg ccgagaaaac cctcatccgg gggatggact
     1981 cgggtttccg ggggagggaa cctttttaag gataccgggc aacggcaaca ccaggaaccg
     2041 gaccgaaatt catttcggcc gggcagaccc tttttcgaag gacctgttcc cattttggat
     2101 cttattggga acaggtcttt tcttgctcca gcgccctggc cgggcgcttt catcgccggg
     2161 agcccagccg aaattcattt cggctgggca gacccttttt cgaaggacct gttcccattt
     2221 tggatcttat tgggaacagg tcttttcttg ctccagcgcc ctggccgggc gctggagacg
     2281 tcgggtcaat gtccggcccc tcccccaccg ggggcgcccc agccggcggg tttcttcaaa
     2341 tagtagatgg ggatgatggt gaccacgaag aggaaggcca tcagccagca catgtggtcg
     2401 aagcccagca cgaaggcctg cttcttgacc cggaaaaaag cctccttggc caccaggctg
     2461 tccggggtgg ccatgcccag ggtgtcggcg aacttgaagg ccatgccgtg cacgctctgg
     2521 gcccattggc tgtagcccat gctcaagggg ctgatgtatt ggcgcaggtg cgcgtaggcc
     2581 aggtcctggg acttgctgaa ca
//
LOCUS       DASTJJ010000247         1376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_403705, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000247 DASTJJ010000000
VERSION     DASTJJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403705"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..278
                     /locus_tag="VFR02_03010"
     CDS             42..278
                     /locus_tag="VFR02_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869455.1"
                     /translation="MIALLLKHGVAMEISNRWKTPYGRLMEKGVKAGLKFSLGSDGHD
                     PAKTCVLDYPKRMMERHRLPADRLFDLSRPLEAA"
     gene            278..1216
                     /gene="era"
                     /locus_tag="VFR02_03015"
     CDS             278..1216
                     /gene="era"
                     /locus_tag="VFR02_03015"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9869456.1"
                     /translation="MENPDITPPSGDFVCGAVAVLGKPNVGKSTFLNAALGQKLAIVS
                     PRPQTTRGSMAGVYQDGDSQIVFYDTPGIHQPQNELSHFMLRQVEEVLEGADAALFLM
                     DARLGVTDEDRLVADWLKKSKGPAWVVMNKSDQVPLTRLGEVADRVRAELGDWPIRSM
                     SAKTGRGVKDLLAEIKRGLPKQPPLFPPEELTDKHLRDLAGELVREQCFLQLKEEIPY
                     GVAVVVEKYEEREPPAPILVQATIYAERDSQKGILIGAGGKQLKSIGSAARREIEALT
                     GRKVFLELWVKVLKNWRKDEAVLRRLGYQPPKKKRR"
     gene            1232..>1376
                     /locus_tag="VFR02_03020"
     CDS             1232..>1376
                     /locus_tag="VFR02_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9869457.1"
                     /translation="MHRIHPKTKLVCTIGPASRSDAMLEKLLLAGMNVARINFAHGSI
                     EQNG"
BASE COUNT          271 a          445 c          447 g          213 t
ORIGIN      
        1 gcgaccccga gacggccttc cccgaatggt aggaggacga attgatcgcc cttctgctca
       61 agcacggtgt ggccatggaa atttccaacc gctggaagac gccctatggg cggctgatgg
      121 aaaagggcgt gaaggcgggc ctgaagttct ccctgggatc cgacggccat gacccggcca
      181 agacctgcgt gctggattat cccaagagga tgatggaacg ccaccggctc cccgccgacc
      241 gcctcttcga cctatcccgt cccctggaag cggcctgatg gaaaatccgg atatcacgcc
      301 cccgtcgggg gactttgtct gcggggccgt ggcggtcctg ggcaagccca acgtgggcaa
      361 gtccaccttc ctgaacgccg ccctggggca gaagctggcc atcgtgtcgc cccggcccca
      421 gaccacccgg ggttccatgg cgggcgtcta ccaggacggc gacagccaga tcgtcttcta
      481 cgacaccccg ggcatccacc agccccagaa cgagctctcc catttcatgc tccggcaggt
      541 cgaggaagtg ctggaaggcg ccgacgcggc cctgttcctg atggacgccc gcctgggcgt
      601 caccgatgag gaccggttgg tggcggattg gctcaaaaaa tccaagggcc cggcctgggt
      661 ggtgatgaac aagtccgacc aggtgcccct gacccgcctg ggcgaggtgg ccgaccgggt
      721 ccgcgccgag ctgggggact ggcccatccg gtccatgtcg gccaagacgg gccggggcgt
      781 gaaggacctc ttggccgaga tcaagcgcgg cctgcccaaa cagccgcccc ttttcccgcc
      841 ggaggaactc accgacaagc acctgcggga cctggcgggg gagctggtgc gggaacagtg
      901 tttcctgcag ctcaaggaag agatccccta cggcgtggcc gtggtggtgg aaaaatacga
      961 ggaacgggaa ccgcccgcgc ccatcctggt ccaggccacc atctacgccg aacgggacag
     1021 ccaaaaggga atcctgatcg gggcgggcgg aaaacagctg aaatccatcg ggtcggcggc
     1081 ccggcgggag atcgaggccc tcacgggccg gaaagttttc ctggaattat gggtgaaagt
     1141 ccttaagaat tggcgcaagg acgaggcggt tttgaggcgg ttgggttacc agccgcccaa
     1201 gaaaaagaga cgctgagccc cggaggcttc catgcaccgt atccacccca aaaccaagct
     1261 ggtctgcacc atcggccccg cctcccgctc cgacgccatg ttggagaagc tcctgttggc
     1321 cggcatgaac gtggcccgca tcaatttcgc ccacggctcc atcgagcaga acggcg
//
LOCUS       DASTJJ010000248         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_892813, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000248 DASTJJ010000000
VERSION     DASTJJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892813"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFR02_03025"
     CDS             <1..354
                     /locus_tag="VFR02_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET9869458.1"
                     /translation="EEALAGMKGGAIVFALSNPDPEVMPEVARRHAAVVATGRSDFPN
                     QINNVLAFPGIFRGALDAGARRITPAMKLAAAAAIAGLAADAASAGYIVPSPLDPRVG
                     PAVAAAVAAAVAAAV"
     gene            complement(510..626)
                     /gene="rrf"
                     /locus_tag="VFR02_03030"
     rRNA            complement(510..626)
                     /gene="rrf"
                     /locus_tag="VFR02_03030"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(843..>1100)
                     /locus_tag="VFR02_03035"
     rRNA            complement(843..>1100)
                     /locus_tag="VFR02_03035"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          147 a          312 c          396 g          245 t
ORIGIN      
        1 gaggaggccc tggcggggat gaaggggggt gcgattgttt ttgcgttgtc gaacccggat
       61 ccggaggtca tgccggaggt ggcgcggcgg catgcggcgg tggtggcgac ggggcgtagt
      121 gattttccga accagatcaa caatgtgctg gcgtttccgg ggatcttccg tggggcgctg
      181 gatgccgggg cgcgccggat caccccggcg atgaagctgg cggccgcggc ggcgattgcg
      241 ggcctggcgg ccgatgccgc ctcggcgggg tacattgtcc cgagtccgct ggatccgcgc
      301 gtgggcccgg cggtcgcggc agcggtcgcg gcagcggtgg ccgccgccgt ctagcggcgg
      361 tgtggccggc ccccggcccc tggcccctgg cccctggccg gaccctggcc gggggccggg
      421 tgtcgggggc ggggtttgtc gtgtggttgt ggtggttaag tgtggggagg ccccaacacg
      481 ggtgtgttgg ggcctctacc cttggtgttg tccggcggtg tcctactctc ccacatcctc
      541 acgaatgcag taccatcggc gctgtgggtc ttagcttccg ggttcggaat gggaccgggc
      601 gtttccccca cgctatgacc accgtaactt ttcctccgca ccccttgccg tggcccgccc
      661 tggcgggggg tggtttgggg gggaatcctg gttggtgtcc tgttgatgga caacagtatt
      721 ttattataca cacacccctg cagggggtgg gtggtggttt ttcccgtggg caacaaggac
      781 ccggccgggt ttgttgttgg ggaaccatat agtgaacgcg agcaggtgtg gtgttggttg
      841 gtgttatcaa gttatcggcc tattagtacc ggtcagcttc acgagtcttt ggtcctcgct
      901 tccacgtccg gcctatcaac ccagtggtct gctgggggcc tcccacaccc taggggtgta
      961 tggaaatctc atcttgaagc gggcttcccg cttagatgct ttcagcggtt atcccatccg
     1021 aacgtagcca atcagcgatg cacttggcag tacaactgac acaccagagg ttcgtccgtc
     1081 ccggtcctct cgtactaagg
//
LOCUS       DASTJJ010000249         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1748268, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000249 DASTJJ010000000
VERSION     DASTJJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748268"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFR02_03040"
     CDS             complement(<1..797)
                     /locus_tag="VFR02_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HET9869459.1"
                     /translation="MTSTAPALALAIAGSEATGGAGAQADLKTFQELGVFGIVNLTCI
                     VSFDPRDNWNHRFVPVDQQVIADQLEATTAAYGAGSGATTVLETVKIGMLGSPATITT
                     VETALKGAGFKNVVLDPVLICKGQEPGHALDTDQALKAQILPLATFVTPNHFEAESLS
                     GMEIHNVGDLKAAARRIHGISGAAVLAKGGVRLSGADAVDVFFDGETLEVLSAPKVGD
                     VAVSGAGCSLAAAVTAELAKGATPLEAARSAKAFVTEGIRNRVVSGAP"
BASE COUNT          181 a          371 c          355 g          185 t
ORIGIN      
        1 ggggcaccgg aaaccacccg gttccggatg ccttctgtga caaacgcctt ggccgagcgg
       61 gccgcttcca acggcgtggc acccttggcc agctccgccg tgacggccgc ggcgagggag
      121 cacccggccc cggagacagc cacgtcgccc accttcgggg cggagaggac ctccagcgtt
      181 tcaccgtcaa agaaaacgtc gacggcgtcc gcgcctgaca gcctcacccc gcccttggcg
      241 agcacggcgg cgccgctgat cccgtggatc ctgcgcgccg ctgccttcag gtccccaacg
      301 ttgtggattt ccatcccgga gagtgattcc gcctcgaagt ggttgggcgt gacaaaggtg
      361 gccaggggca ggatctgcgc cttcagtgcc tggtcggtgt cgagggcgtg gccgggctcc
      421 tggcccttgc agatcagcac cgggtccagc accacgttct tgaagcccgc ccccttcaag
      481 gcggtttcca ccgtggtgat ggtggcgggg ctgcccagca tgccgatctt caccgtctcc
      541 agcaccgtgg ttgccccgga accggcaccg tacgctgcgg tggtggcttc cagctggtcc
      601 gcgatgacct gctggtccac cggcacgaaa cggtggttcc agttgtcccg gggatcaaag
      661 gagacgatgc aggtaaggtt cacaatgccg aagacgccca gctcctggaa cgtcttcagg
      721 tcggcctggg cgccggcgcc gccggtcgcc tcggagccgg caatggccag ggcaagggcg
      781 ggtgctgttg aagtcatgtc tccatcctgc catccggggc ggaagaaggc ccggcagcgg
      841 ccactacctc catgacttgc catgacgggc catgtgggtg cctccccgta acatcggcag
      901 ttaacagcac agcggaacca cttcaccgca agcaaggggc accggaatga aacgcaaggc
      961 accgtactcg cagcatgcac agaccatgct gcgcaccacc catgtcctgt ggggagcaat
     1021 actggacgca ctggcgttgt tcttccttgt cctcggcctg ggcggcagct ccgccgggtg
     1081 gcttgtccct gc
//
LOCUS       DASTJJ010000250         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_770482, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000250 DASTJJ010000000
VERSION     DASTJJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770482"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFR02_03045"
     CDS             complement(<1..233)
                     /locus_tag="VFR02_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9869460.1"
                     /translation="MIQPYQGRSPAFGKNVRVHDAAVVIGRVTLADDVSLWPGAVLRG
                     DMDGIQVGARTNIQDGAVFHTDTGHPAVIGEDCV"
     gene            complement(435..887)
                     /gene="bcp"
                     /locus_tag="VFR02_03050"
     CDS             complement(435..887)
                     /gene="bcp"
                     /locus_tag="VFR02_03050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET9869461.1"
                     /translation="MIKEGQKAPDFNLDDDQGKKVSLKDLKGKTVVLYFYPKDMTPGC
                     TQEACDFRDNWTAVKKKGAVVLGVSADPVKRHKSFKEKYKLPFPLLSDEGLKMLKAYG
                     VWKKKKFMGREFMGIVRTTLIIGPDGKIKKLWSPVSVKGHADDVLENL"
     gene            1264..1929
                     /locus_tag="VFR02_03055"
     CDS             1264..1929
                     /locus_tag="VFR02_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869462.1"
                     /translation="MNFYFSLYQLIETVERLNHDIQHLAGRALRISPRDARANRELLE
                     QCETKLGEVKHLLNNKTHISKITLRKAALLFQILEVSHRRVMNLAAATSAVGLEDDQS
                     VKALWQLLAMGQRHYQTLVKTLNDMTGIPNSLVLQPETGAPLADFAAALKKEWALQQE
                     TLKQLERAIEVFGEEQELAETLSAIRKELLEAKKRLKHLSPALAHAPEKQEAVPAPAA
                     VKN"
BASE COUNT          413 a          617 c          609 g          404 t
ORIGIN      
        1 acgcagtcct cgccgatgac ggcgggatgg cccgtgtcgg tgtggaacac cgcgccgtcc
       61 tggatgttgg tgcgggcgcc cacctggatc ccgtccatgt cgccgcgaag caccgccccg
      121 ggccagaggc tcacgtcgtc ggcgagggtg acccggccga tgaccaccgc cgcgtcatgc
      181 acgcgcacgt tcttgccgaa ggcgggggac cggccttgat agggttggat catggggtgg
      241 ttgccgttct atccgattta tttgtcattg cgaggaatgc cgccgaatag gcggcgttca
      301 tgacgaagca aacccagttt aaacaaagct ctggctttaa gaattcacgg cgaaagtcgt
      361 atcagaggcc atgaactggg gttgcttcgt cgctaaaacc aatgaacgac gctcctcgca
      421 atgacacaat aagcttacaa attctccagc acgtcatccg cgtggccctt cacgctcacc
      481 gggctccaga gtttcttgat cttcccgtcg gggccgatga tcagggtggt gcggacgatg
      541 cccatgaatt cgcggcccat gaacttcttt ttcttccaaa ccccataagc tttcagcatc
      601 ttgaggccct cgtccgagag cagggggaag ggcagcttgt acttttcctt gaagctcttg
      661 tgccgcttca ccgggtcggc gctcacgccc aagaccaccg cgcccttctt cttcacggcg
      721 gtccagttgt cccgaaagtc gcaggcttcc tgggtgcagc cgggggtcat gtccttggga
      781 taaaagtaga ggaccacggt ctttcccttc aggtccttca gggatacctt ttttccctgg
      841 tcatcgtcca aattgaagtc cggggctttt tggccttctt tgatcatgga agactccttc
      901 gggggaattg gcgcccatga tgggcccggc ttccggggaa gacaagcccc ttgggccccc
      961 ttccaaacca cgcgaaaacc catatttttc agggacaaaa gtgggttagg tgcgggtcaa
     1021 atctgtgtaa aagttgacaa gtattggaca caaatgacct tttaaagata cccgaccaat
     1081 aagtaggttt aagattaaac gcttgaaaat cgttaaaaat taacatgttt ttagtgtttt
     1141 tagtaacttc tggtttaatt cggagggtac aaggagtata gtctttccat tcaacgggtt
     1201 tggctttgcg ggtcctggaa ggccgatggg cgccgggcgg cggagcagaa agggagtcgg
     1261 gccatgaatt tttacttctc gctgtaccag ctgatcgaga ccgtggagcg cttgaaccat
     1321 gacatccaac atttagccgg gagggccctg cgcatcagcc cccgggacgc ccgcgccaac
     1381 cgcgaactgc tcgaacagtg cgagaccaag ctgggcgagg tcaagcacct gctcaacaac
     1441 aagacccaca tcagcaagat caccctgcgc aaggccgccc tgctcttcca gatcctggaa
     1501 gtgagccacc gccgcgtcat gaacctggcc gccgccacct cggcggtggg cctggaggac
     1561 gaccagtcgg tgaaggccct ctggcagctc ctggccatgg gccagcgcca ctaccagacc
     1621 ctggtcaaga ccctgaacga catgaccggc atccccaaca gcctggtgct gcagcccgag
     1681 accggcgccc ccctggccga cttcgccgcc gccctcaaga aggaatgggc cctgcagcag
     1741 gagaccctga agcagttgga acgcgccatc gaggtcttcg gcgaggagca ggaattggcc
     1801 gagaccctgt cggccatccg caaggagctg ctggaggcca agaagcgcct caagcacctc
     1861 tcgccggccc tggcccacgc ccccgaaaag caggaggccg tcccggcccc ggcggccgtc
     1921 aagaactgac cggacggacc ttcagcccat ggcttcccat ttgacccgcc gccccttggg
     1981 ggcggccctt tccgtgcggg catgagcccc cggcgtgggg gggggggggg gggggggggg
     2041 ggg
//
LOCUS       DASTJJ010000251         1473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1015349, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000251 DASTJJ010000000
VERSION     DASTJJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015349"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1473
                     /locus_tag="VFR02_03060"
     CDS             <1..>1473
                     /locus_tag="VFR02_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869463.1"
                     /translation="FLAIYPQPIARWVARWKPRLGKDLDDWMEKGMDLLGAWGRPRGP
                     VHWALVVGLVLWGILVAASWAHWTEAGSKALAARGLGAALLALALAGLWTRRWFFAAA
                     GALVLGFWGLLMVFGVLPLFQSHSPVLGLGFFTLIWAAAFLNLGFAAKLRPQGALSFT
                     FLMAGLFFLVTATLEIFVMKEYLGGDYMRNNSLFKFGINAWTLASVAAGVLLPLVWER
                     TGALLKSVRTESPAARKALYLAGFVVLSWMVHALLDEAVFSQGWTASYVLNLAFVLGI
                     LVWAYLEEWAPVWVLASVAVVLVPLLVLPLFSVSDYTSVLYVVQRLFGRVDSGFLFPT
                     AIVLIVMIAVDFLRERDPRRGLRSFRTGWGSLVLLLGAMALVYPLAGTWRKCHGFFSE
                     SRARAMGYHEAPTLNGLHYLAQVDPDDAAAIRFLNAHVPDQPCLIEAVGLGYNTWGSR
                     YSIFTGIPALMGWDGHVREWVGQEEDREISMRYEATETIF"
BASE COUNT          184 a          433 c          508 g          348 t
ORIGIN      
        1 gttcctggcg atctatccgc aaccgatcgc gcgttgggtc gcccgttgga aaccccgcct
       61 gggcaaggac ctggatgact ggatggagaa gggcatggac ctgttgggcg cctggggccg
      121 gccccggggg ccggtgcatt gggccctggt ggtcgggctg gttttgtggg ggatattggt
      181 ggcggcctct tgggcccatt ggaccgaggc aggctccaag gccctggcgg cccggggatt
      241 gggcgcggcc cttttggcct tggccctggc gggcctttgg acccgtcggt ggttctttgc
      301 cgcggcgggg gccttggtgt tgggtttttg gggtttgctg atggtctttg gggtcctgcc
      361 cctctttcaa tcccactcac cggtcctggg gctgggtttc ttcaccctga tctgggccgc
      421 ggccttcctg aacctggggt tcgcggccaa gttgcgcccc caaggcgcgc tctccttcac
      481 ttttttgatg gccgggctgt tcttcctggt gacggccacg ttggaaatat tcgtgatgaa
      541 ggaatacctg ggcggggact acatgcgcaa caactcgctc ttcaagttcg ggatcaacgc
      601 ttggaccctg gcctcggtgg cggccggggt gttgctgccc ctcgtgtggg aaaggaccgg
      661 ggcgctcctc aagtccgtcc ggaccgagag cccggccgcg cgcaaagcgc tttacctggc
      721 gggattcgtg gtcctttcct ggatggttca tgcgctcctc gacgaggcgg tcttttccca
      781 gggttggacg gcttcctatg tgttgaacct ggccttcgtc ctgggcatcc tggtctgggc
      841 ttatctggaa gaatgggcgc cggtttgggt cctggcctcg gtggcggtgg tgctggtccc
      901 tttgctggtc ctgccgctct tctccgtgag cgattacacg tccgtcctgt acgtggtcca
      961 acggcttttc ggcagggtgg attcgggatt tttgttcccc accgccatcg tcctgatcgt
     1021 gatgatcgcg gtcgatttcc tcagggaacg cgatccccgg cgcggcctgc ggtcgttccg
     1081 gacgggttgg ggttccttgg tgttgttgct gggggcgatg gcgttggtct atcccctggc
     1141 cggcacctgg cgcaaatgcc acggtttctt ctctgaaagc cgggcccggg ccatgggcta
     1201 ccacgaggcg ccgaccttga acgggctcca ctatctcgcc caagtggacc cggacgacgc
     1261 ggccgccatc cgattcctga atgcccatgt cccggaccaa ccttgcttga tcgaggcggt
     1321 cggattgggt tacaacacct ggggctcccg ctattcgatc ttcacgggca tcccggcctt
     1381 gatggggtgg gacggccatg tgcgcgaatg ggtgggccag gaggaggacc gggagatcag
     1441 catgcgttat gaggccaccg agaccatctt caa
//
LOCUS       DASTJJ010000252         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_525876, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000252 DASTJJ010000000
VERSION     DASTJJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525876"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..1088
                     /locus_tag="VFR02_03065"
     CDS             609..1088
                     /locus_tag="VFR02_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HET9869464.1"
                     /translation="MGFARLLAVGVIVVSAATGCATVAPTPPRPTPTAAGSGPTATHT
                     TSWTLSGTFVPQGAPTRGQVRITEKPGSLVLTLSDFSTGPGQDLYIDFNPGAMTRNAA
                     GDNVVEDPNTFQVVALKDITGTQSYDLSYLIPVWPQVRSVTIFSSKSREAFGTANLR"
     gene            1247..2065
                     /locus_tag="VFR02_03070"
     CDS             1247..2065
                     /locus_tag="VFR02_03070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9869465.1"
                     /translation="MPTGDAAHRHRELAESFGKDAASYDHARPRYPAALVDAVVERIR
                     GHTILDVGIGTGISAEPFRDRGLAVLGIDPDPQMARLARAKGFNVEVARFEDWPAAGR
                     TFDAIAAGQAWHWVDPTAGAAKAACLLRPGGVLALFWNAGRPPAELATGFATAFDALH
                     TGLPFNPWAALAGADPYGAIIDAAAAGLRTTGAFGAPERLSFKWQATATRDEWLGQAA
                     TSGGINRLPKDKRKALVNSMGAAIDAAGGNLTIDYTTVAAISERLPIRSNVAAE"
     gene            complement(2121..>2882)
                     /locus_tag="VFR02_03075"
     CDS             complement(2121..>2882)
                     /locus_tag="VFR02_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9869466.1"
                     /translation="LSGPGFSTIPTALSQGTLSTATLAADAKNWGNQLRWAGLKVDLA
                     PVLDTVTQAFAPSNAPIGYYEREYGYTPQAVSAKGNAFLAGIKAGYVMPTVKHFPGLG
                     RVTGNTDVTGNVHDTQTTINDPNLLPFQTAINNGARWVMVSSAYYDRIDARPGKIAPF
                     STVVMGTMLRTNAKFTGIIISDDLCNAAQLSPWSWAVRANNFINAGGTMILCANPNAV
                     PYMYAAVLQLAQQRPDFLAKVNAAVLTILTVKHGQ"
BASE COUNT          493 a          965 c          951 g          473 t
ORIGIN      
        1 ggccaccgag cccgccccgg agggcaccgc cgccgcgtac gccgcgcacc ccggacccac
       61 caaaccaccc gcagcagcca tcggggacgc cgcggccggc gccgaggtcg ccatcggggc
      121 gtgcgtggtt gccggggccg ccgtcgtccc cccgctgcac gcgctcaacc ccatggccgt
      181 gacggccaga aggcccatcc ccaaacccag gcccttacgg gtgcgaaact tcgtgcttgc
      241 attcatgatg attctccctg ccctggaaag ccatcggcct cccctttatg cttcccgcac
      301 cgccaccgaa aaaccccgga aacgccacgg actgtcaacc agtacttcgg accaccaccc
      361 ccaccggatt ggcggccggc ccggaaagtt ttttcactgc cgcgccagcc ctgccgtggg
      421 cgcgcggctt ccaggaatgc ggcgacacgg atttcgcttc gagggccaac tcccccaggt
      481 cctgatcggg caatggggct tgggccagga cggccgtcaa tagatggcct gccagggttc
      541 ccggcgggct agtatgcaat ggaaccacgt agtagcgggg aatcgtaaaa gcactggaaa
      601 tggggatcat gggatttgcg cgcctgcttg ccgtgggggt cattgtcgtt tcagccgcga
      661 caggatgcgc caccgtggca ccgaccccgc ccaggcccac acccacggct gccggctccg
      721 ggccgacagc gacgcataca acatcatgga cgctttcggg cacgttcgtc ccgcaagggg
      781 ccccgacgag agggcaggtg cggatcactg aaaagccggg cagcctggtc ctgacgttgt
      841 cggacttttc cacgggtccc gggcaggacc tgtacattga cttcaacccg ggagccatga
      901 cacgcaacgc ggccggggac aatgtcgtgg aggacccgaa cacgttccag gtggtggcac
      961 tgaaggacat tacgggaaca cagagctatg acttgagcta tttgatcccc gtgtggcccc
     1021 aggtccgcag cgtcaccatc ttcagcagta agtcgcgcga ggccttcggc acggcaaatc
     1081 tgcgctaagg gcaggaccgc gaatcgcacc tgcgtttcgg ggcggggaag cgtgcggcga
     1141 caccgctaaa gccatccgag gggagtgtgc gtcttgccgc cgtcctgtcc ccaatgggcg
     1201 catgcctgtt tagaatggcc gcatggtccc tccagcccca ccgatcatgc ccaccggaga
     1261 cgctgcgcat cggcaccgcg aactcgcaga gtcgtttggc aaggatgctg ccagctatga
     1321 ccacgcccgc ccacggtacc cggcagcact cgttgatgcc gtcgtcgaac ggatccgggg
     1381 gcacacgatc ctcgacgtgg ggatcgggac cggaatctcc gcagagccct tccgcgaccg
     1441 cggactcgcc gtgctcggga tcgaccccga cccgcagatg gccaggcttg cacgggccaa
     1501 gggatttaac gtagaggtcg ccagattcga agactggccg gccgccggac ggacgttcga
     1561 cgccatagcc gctggacagg cctggcattg ggtcgacccc accgcgggcg ccgcaaaggc
     1621 cgcttgcctc ctccggccag ggggcgtcct ggcgctcttc tggaacgctg gacgcccgcc
     1681 tgcagaactc gccacggggt tcgcgacggc tttcgacgct ctccacaccg ggttgccgtt
     1741 caatccctgg gccgctttgg ccggggccga cccgtatggg gcaatcatcg acgctgccgc
     1801 ggcgggcctt cgcaccactg gggcattcgg cgcgcccgaa cggctctcct tcaagtggca
     1861 ggcgaccgcc acccgcgacg agtggcttgg gcaggcggcc acctcgggcg gcatcaaccg
     1921 cctcccgaag gacaaacgga aggcgctcgt caacagcatg ggtgccgcca tcgacgccgc
     1981 aggcggcaat ctcaccatcg actacaccac cgttgcagcc atcagcgagc ggctgcccat
     2041 tcggagcaac gtggccgccg aataaaccag gcggaaccag ggccggcacg cactccacca
     2101 gttttttggc ggccgggcgg tcattgtccg tgcttgaccg tgaggatggt gagcaccgcg
     2161 gcgttgacct tggccaggaa gtcggggcgc tgctgggcca gctgcagcac cgcggcgtac
     2221 atgtagggga cggcgttggg gttggcacac aggatcatgg tgccgccggc gttgatgaag
     2281 ttgttggccc gcacggccca gctccagggc gaaagctgtg cggcgttgca gaggtcgtcg
     2341 gagatgatga tgcccgtgaa cttcgcgttg gtccgcagca tcgtgcccat gacgaccgtg
     2401 gagaacggtg cgatcttgcc gggccgggca tcaatcctgt cgtagtacgc actggagacc
     2461 atcacccacc tggccccgtt gttgatggcc gtctggaacg gcagcaggtt ggggtcgttg
     2521 atggtggttt gggtgtcgtg cacgttgccc gtcacatccg tgttgccggt gaccctgccc
     2581 agcccgggga agtgcttgac cgtgggcatc acgtagccgg ccttgatgcc ggccaggaac
     2641 gcgttgccct tcgcggagac ggcctgcggc gtgtacccgt attcacgttc gtagtacccg
     2701 atcggggcgt tggacggggc aaaggcctgc gtgacggtgt caaggacggg ggcgaggtcc
     2761 actttcaggc ccgcccagcg cagctggttt ccccagtttt tggcgtccgc cgccagggtg
     2821 gccgtggaca gcgtgccctg ggacagcgcc gtcggaatgg ttgaaaatcc cggcccgctg
     2881 ag
//
LOCUS       DASTJJ010000253         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1748789, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000253 DASTJJ010000000
VERSION     DASTJJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748789"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1373
                     /locus_tag="VFR02_03080"
     CDS             228..1373
                     /locus_tag="VFR02_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9869467.1"
                     /translation="MQTLKVGVVGAGMIGDHHARLWAKVPGVTVAGIVEVDQAKARAM
                     ADRLKIPAFGSLRELKRRTGAQVASLCVPSALHAKVGLEAVREGMHLFVGKPLDINIS
                     RAKELLRAARSKGLRLAVSSQKRWAPGSLKIRELVAAGALGRIFLVRAIVPWYRSQKY
                     YDQGAGRGTYRSDGGGALIIHAIHMVDLALHLTGKEPIWAGASSGTFTHRIEVEDTLV
                     GHLRMSDGTLGQVLAGTSLYPGFADRVEIYGEKGTVHWEGGDLSFAMFNGGKVSDYGT
                     FLTYGKIPFLKKFADGLQVGILGPLREPWKFVRARLKAVDWPLLGRSVSAFDESFLAQ
                     LRDAGEFFRTGKGSIVTGRSALRTLAVIEALYRSARTHREERVEPVR"
     gene            complement(1408..>2422)
                     /locus_tag="VFR02_03085"
     CDS             complement(1408..>2422)
                     /locus_tag="VFR02_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869468.1"
                     /translation="LRLLYKPDTVLTVEAAEELDPLYRSGSGGAAADFLPPVSAYRLI
                     DFSNPLAGSADGNGGKLSQDLDRLSASLHLGRLDIVLGRQVVAFGSARLVNPTDVLAP
                     FHFQTLDQENRSGVDAMRLRYAWGEFGELDGGYVAGKDARWDDSAAFLRLHFRPGGLD
                     SSVLAMAFQENFLAGLDLQGSLGGAGWWAEGAWVEPHLPGGGYGRLSTGADYYFTGDL
                     DASLEYHFNGAGKASPSDYLDLDPTAYGAGTVYLLGRHYFSPSLSWQADPLLSLQAQA
                     VMNLGDGSWWGLVHAEYNALENLYADLGLAQPIGAGPQGSLPRSEFGLYPAFQYADLR
                     LYF"
BASE COUNT          468 a          789 c          788 g          377 t
ORIGIN      
        1 taaaaaatcc cacaaaaccc atgaccgggc gtcgttttgg acgctcgccc gctcaaacga
       61 gatcggatcc ttgcaaccgc ttcaggttca agaccgtcca acaaaatggc ggcggcccac
      121 aagccacaag aagccatggc cggaaggcgt tggaagggcg tcccctttcc aatcttagag
      181 ggtggtccat ccgacccgcg agaaaacgac ccaacggaga agctatcgtg caaactctga
      241 aggttggagt ggtgggggcc gggatgatcg gggatcatca tgccaggctt tgggccaagg
      301 tgccgggcgt gacggtggcg gggatcgtgg aggtggacca ggccaaggcc cgggccatgg
      361 cggaccgcct caagatcccc gcgttcggtt ccctccggga actcaagcgc cggaccggcg
      421 cgcaggtggc cagcctttgc gtcccttcgg cgctccacgc gaaggtcggc ctggaggccg
      481 tccgggaagg catgcacctc ttcgtgggaa aacccctgga catcaacatt tcaagggcga
      541 aagaattgct ccgggccgcc cgaagcaagg gcctgcgttt ggccgtttcg agccaaaaac
      601 gctgggcccc cgggagcctt aagatccggg aactggtggc cgcgggggcc ctgggcagga
      661 tcttcctcgt ccgcgccatc gtaccctggt accgcagcca aaagtattat gaccagggcg
      721 ccgggcgggg gacctaccga tcggacgggg gcggggccct catcatccac gcgatccaca
      781 tggtggacct ggccctccac ctgacgggca aggaaccgat ctgggccggc gcgtcctccg
      841 ggaccttcac ccaccgcatc gaggtggagg acaccctggt aggccacctg cgcatgtcgg
      901 acggcaccct gggccaggtg ctggccggca cttccctgta ccccgggttc gccgaccggg
      961 tggaaattta cggagaaaag ggcaccgtcc attgggaggg aggggacctg tccttcgcca
     1021 tgttcaacgg cgggaaagta tccgattacg ggactttttt gacctacgga aagatcccct
     1081 tccttaaaaa attcgccgat gggctccaag tgggcatcct ggggcccctc cgggaaccct
     1141 ggaaattcgt ccgcgcgcgg ctcaaggcgg tcgattggcc cctgctgggg aggtccgtca
     1201 gcgcgttcga tgagagtttc ttggcccagt tgcgggacgc cggggaattt ttcaggaccg
     1261 gcaaaggatc catcgtcacc ggccgatcgg cgttgcggac cctggcggtc attgaggccc
     1321 tctatcggtc cgcccggacc catcgggaag aaagggtcga gcctgtccga tgacatccca
     1381 aggaccgcct ttcccgtcgc cctccgctca aaaataaaga cgcaggtccg cgtattggaa
     1441 ggcgggatag aggccgaatt cggaccgggg cagggatccc tggggccccg cgccgatggg
     1501 ttgggccaac cccaggtccg cgtagagatt ctccagggcg ttgtattcgg cgtgcaccaa
     1561 accccaccag gacccgtcac cgaggttcat caccgcctgg gcctgaagcg acagcagcgg
     1621 gtcggcctgc caggacaggc tgggggaaaa gtaatgtctt cccaataaat agaccgtccc
     1681 ggcgccgtag gccgtggggt caaggtccag gtagtcggaa ggggaggcct tcccggcccc
     1741 gttgaagtgg tattccagcg acgcgtccag gtcgccggtg aaatagtaat ccgcccccgt
     1801 ggaaaggcgg ccatagccgc cgccgggaag gtggggttcc acccaggccc cctcggccca
     1861 ccagccggcc ccgcccaggg acccctgaag gtccaacccc gccaggaaat tctcctggaa
     1921 ggccatcgcc aacaccgagc tgtccaagcc ccccggacgg aagtggagcc tgaggaaggc
     1981 cgcgctgtcg tcccatcggg cgtccttccc cgccacgtaa ccgccgtcca actcgccgaa
     2041 ttccccccag gcgtaacgca gccgcatggc gtccacgccg gaccggttct cctggtccag
     2101 ggtctggaaa tggaaggggg ccagcacgtc cgtgggattg accaaccgcg ccgagccgaa
     2161 ggcgaccacc tgacgcccca ggacgatgtc cagcctgccc aaatgcaggg aggcggaaag
     2221 ccggtcgagg tcctgcgaca gcttcccacc gttcccgtcc gccgaacccg ccagggggtt
     2281 cgaaaagtcg atcagccggt aggcggagac gggcggcagg aagtccgcgg cggccccgcc
     2341 ggaccccgaa cggtacaagg gatccagctc ctccgccgct tccaccgtga ggaccgtgtc
     2401 gggtttgtac aacagccgca gg
//
LOCUS       DASTJJ010000254         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1138157, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000254 DASTJJ010000000
VERSION     DASTJJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138157"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1075
                     /locus_tag="VFR02_03090"
     CDS             245..1075
                     /locus_tag="VFR02_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET9869469.1"
                     /translation="MTTIRLAHSPDADDAFMFYGLAKGKIDTEGLTYEHVLKDIQTLN
                     EWAREGRMEVTAISAHAYAYVKDKYAVLTHGGSIGKNYGPQVVAKAKVSLESLKGKRI
                     AVPGKLTSAYLALKLRFPDFEDVVVPFDEIMDEVAAGKVDAGLLIHEGQLTHAEKGLV
                     KLLDLGEWWYQEKKLPLPMGLNAIRRDLPEDVQRKASKHLKQSIQYSLTHRDEALDYA
                     LSFGRGLPRAVADQFVGMWVNERTVDMGEEGRTAIREFLRLGEERRLIPPVGQVEFVD
                     "
     gene            1081..1866
                     /locus_tag="VFR02_03095"
     CDS             1081..1866
                     /locus_tag="VFR02_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET9869470.1"
                     /translation="MQPLQVGAALIYRDGKYLITQRKAEDSFGGFWELPGGKREPGED
                     MEDCVRRELKEELDLDVRIQGFFRVVVYPYPHRLVKLNIYWCTLDPDAVPKAIECQAY
                     AWVDPKDLPSYSFPEADQELVRDLAQKPAGMEHFPRAGAYADEYLEFIRLFNRGFYFE
                     SHELLEELWHPAQGADRVHYQALIQLAVACRHLKHGNWAGALGLFAKARSKWSQLPAR
                     HLGLDLRALEAAMAPVFQALQDALGAGKTPGTLPPIPAIPLPA"
     gene            complement(2006..2914)
                     /gene="queG"
                     /locus_tag="VFR02_03100"
     CDS             complement(2006..2914)
                     /gene="queG"
                     /locus_tag="VFR02_03100"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9869471.1"
                     /translation="MTLAGLTQSLKARAAELGFELSGAAPARPPEGYPYFLDWLQKGY
                     AGEMGYLERRKEERGDPARVLPGARSIFCVALSYRPAEPHFPLLGRHPISCYAWGRDY
                     HEVLGEKLEALSDLLRELAPGARAKWYADTGPVLEKDHAAAAGLGWIGKNTLLLNKKF
                     GSFLFLGEILTDVELAYDEPTANLCRSCTACLEACPTGALEEAGVLNATRCISYLTLE
                     HRSPFPQAPELSGYLAGCDLCQSVCPYNQDAPVGREAGFQPRLEVLGLTREGAAAMGE
                     GEFKAFTQDSALERVKYPMWKRNAQT"
     gene            complement(2911..3447)
                     /locus_tag="VFR02_03105"
     CDS             complement(2911..3447)
                     /locus_tag="VFR02_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869472.1"
                     /translation="MPEYKILPPGGDLPGPAELLAGLQERGFSLEVNLSGPPERWEAL
                     RFYQDGPAEVECFLIRDEEAGALTASTPEGSPERAREILVNLADLILRSCGGTLEDPQ
                     TKRTFTAGDFREHQPLLKPRARAAAGWAWPAFAWAVTAASLAAWLGLPESLRAPALAV
                     AALSLLSAAGLTWSALEK"
     gene            complement(3686..4150)
                     /locus_tag="VFR02_03110"
     CDS             complement(3686..4150)
                     /locus_tag="VFR02_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9869473.1"
                     /translation="MKAIWLDTNESLSPEQLNAQGVTYERFPTDETAYKGPLEQVMKE
                     RGYVTMDQVKMWPDMPNFDALCQKFMGEHLHNDDEVRFVLAGSGVFEIRGEGDRWMKV
                     TVEPEDFILVPANRFHRFYLTDEKTIHCVRLFKDQSGWAPLYRKDLEAAGKN"
     gene            complement(4211..>4736)
                     /gene="mtnB"
                     /locus_tag="VFR02_03115"
     CDS             complement(4211..>4736)
                     /gene="mtnB"
                     /locus_tag="VFR02_03115"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HET9869474.1"
                     /translation="EDGRIYMAPSGVQKERLAPGDIFVLDGEGAITEEPGRGLKLSQC
                     SPLFMHAYKIRNAGAVLHSHSLNAVSVTLLFDKHFRIKNMEMEKGLEGFGAFDTLEVP
                     IIENTAYECDLADSLEEAIRNNPKSHAVLVRRHGVYVWGKDWKQAKTHAECYDYLFEA
                     ACRMKQLGIDPTRV"
BASE COUNT          813 a         1522 c         1575 g          826 t
ORIGIN      
        1 gcggccgcca cctgaacgtg cgggtcatcg cggtggccac cggcccctat ccccaagagg
       61 tgctgcgggc cgagggggcg gatttcgtct tccccgacct gaccgaccgg ccccgggtgc
      121 tggaagccat ccaggcttcc ctctaggcct tttcgccccc taaaaacttg gttgcaaaat
      181 gccgcttgag ccgggaagat acgggtccga gcggcctttt tccattcccg accgaggacc
      241 ttccatgacc accatccgat tggcccacag ccccgacgcc gacgacgcct tcatgttcta
      301 cggcctggcc aagggcaaga tcgacaccga gggcctgacc tacgagcacg tgctcaagga
      361 catccagacc ctcaacgaat gggcccggga gggccgcatg gaggtcacgg ccatctcggc
      421 ccacgcctac gcctacgtga aggacaagta cgccgtgctc acccacgggg gcagcatcgg
      481 caagaattac gggccccagg tggtggccaa ggccaaggtg tccctggaat cgctgaaggg
      541 caagcgcatc gcggtgccgg gcaagctcac cagcgcctac ctggccctga agctgcggtt
      601 ccccgatttc gaggacgtgg tggtgccctt cgacgagatc atggacgagg tggcggcggg
      661 gaaagtggac gcggggctgc tcatccacga gggccagctc acccacgccg agaagggcct
      721 ggtcaagctg ttggacctgg gcgaatggtg gtaccaggaa aagaagctgc ccctgcccat
      781 gggcctcaac gccatccgcc gcgacctgcc cgaggacgtg cagcgcaagg cctccaagca
      841 cctcaagcag tccatccaat actccctcac ccaccgggac gaggccctgg actacgccct
      901 gtccttcggc cggggcctgc cccgggccgt ggcggaccag ttcgtgggga tgtgggtcaa
      961 cgagcgcacg gtggacatgg gcgaggaggg ccgcacggcc atccgcgagt tcctcaggct
     1021 gggcgaggaa cgccggctca tcccgccggt gggccaagtc gagttcgtgg actaaggccc
     1081 atgcaacccc tgcaagtggg cgcggcgctc atctaccgcg acggcaagta cctcatcacc
     1141 cagcgcaagg ccgaggactc cttcgggggc ttctgggaac tgccgggggg caagcgggaa
     1201 cccggcgagg acatggagga ctgcgtccgg cgggaactga aggaggaatt ggacctggac
     1261 gtgcggatcc agggtttctt ccgagtggtg gtctacccct acccgcaccg gctggtgaag
     1321 ctcaacatct attggtgcac cctggacccc gacgccgtgc ccaaggccat cgaatgccag
     1381 gcctacgcct gggtggaccc caaggacctg ccttcctatt ccttccccga ggccgaccag
     1441 gaactggtgc gggacttggc ccaaaagccc gccggcatgg agcattttcc ccgggccggc
     1501 gcctacgccg acgagtactt ggagttcatc cggctcttca accggggttt ctatttcgag
     1561 agccatgaat tgttggagga actctggcac cccgcccagg gcgcggaccg ggtccattac
     1621 caggccctga tccaactggc cgtggcctgc cgccacttga agcacggcaa ttgggccggg
     1681 gccctggggc tcttcgccaa ggcccgttcc aaatggtccc aactgcccgc ccggcacctg
     1741 ggtctggacc tgcgggcgct ggaagccgcc atggcgccgg tcttccaggc gctgcaggac
     1801 gccttgggcg cgggcaagac ccccggaacc ctgcccccca tccccgccat ccccctgccg
     1861 gcttaaggcc ctggaaccac agatgcacac ggatgcacgc ggataaagcc ttgtgtcgaa
     1921 agtccgatcg aattttcagt cgttgttcag gatgaaattt tgatctgtga tcatccgtgc
     1981 gcatccgtgg ataaaaggcc ttgccttagg tctgcgcgtt ccttttccac atcgggtatt
     2041 tcacccgctc caaagcgctg tcctgcgtga aggccttgaa ctcaccctcc cccatggcgg
     2101 ccgcgccttc ccgcgtgagg cccagcactt ccaaccgggg ttggaatccc gcttcccggc
     2161 ccacgggcgc gtcctggttg taagggcaga cggactggca gaggtcgcag cccgccaggt
     2221 agccggaaag ctccggggcc tgggggaagg gcgagcggtg ttccaaggtg aggtagctga
     2281 tgcagcgggt ggcgttcaag acccccgctt cctccagcgc gcccgtgggg caggcttcca
     2341 ggcaggccgt gcaggaacgg cagaggttgg ccgtgggttc gtcataggca agttccacgt
     2401 cggtgaggat ctcccccaag aagaggaagg acccgaactt cttgttcagc agcagggtgt
     2461 tcttgccgat ccagcccagc ccggccgcgg ccgcgtggtc cttctccagg accgggccgg
     2521 tatccgcgta ccacttggcc cgcgcgccgg gggccagttc ccgaagcaag tccgagagcg
     2581 cctccagttt ttcccccagc acctcgtgat agtcccgacc ccaggcgtaa caggagatgg
     2641 gatgccggcc caggaggggg aagtggggtt cggcggggcg gtagctgagg gccacgcaaa
     2701 agatcgaccg ggcgcccggc agcacccggg ccgggtcgcc ccgctcttcc ttgcgccttt
     2761 ccaggtagcc catttccccg gcgtagccct tttggagcca gtccaggaaa taagggtagc
     2821 cttccggcgg ccgggccggc gccgcccccg aaagctcgaa acccagctcg gcggcccggg
     2881 ccttgaggct ttgggtaagg ccggcgaggg tcatttttcc agggcgctcc aggtgaggcc
     2941 ggccgcgctc agcagcgaca aggcggccac cgccagggcg ggcgcgcgca gggactcggg
     3001 caggcccagc caggcggcca ggctggccgc cgtcacggcc caggcgaagg cgggccaggc
     3061 ccagcccgcg gcggcgcggg cgcggggctt gagcagcggc tggtgttccc ggaagtcccc
     3121 ggcggtgaag gtgcgcttgg tttggggatc ttccagggtg ccgccgcagg aacgcaggat
     3181 caggtcggcg aggttcacca ggatctcgcg ggcccgttcg ggggaaccct cgggtgtgga
     3241 agcggtgagc gcccccgcct cctcgtcccg gatgaggaag cattccacct cggcggggcc
     3301 gtcctggtag aaacgcaggg cctcccaccg ttcgggcggg ccgctcaggt tcacctccag
     3361 ggaaaaaccg cgttcctgca ggccggccag cagctcggcg gggccgggca ggtcgccccc
     3421 tggggggagg atcttgtatt cgggcaaggc gggctcctgg gtcaaaataa gatggaattg
     3481 ttcacttcac caagcaaagc ttgtttttaa caatgatgaa cgcagatgga cgccgatgtt
     3541 ggatcgaaac agacgaaaga tcgggtttta tctgcgttca tcggcgtcca tcagtgcaaa
     3601 tgtggttttg ttcgctttgc caaagacata gttccgaaat aaaaaaggcg ggcttgaagc
     3661 ccgccctcgc gttgacggct ggggatcaat ttttcccggc ggcttccagg tccttgcggt
     3721 agagcggcgc ccagccggac tggtccttga acaggcgcac gcaatggatg gtcttctcat
     3781 cggtgaggta gaagcgatgg aagcggttgg cgggcaccag gatgaagtcc tcgggctcga
     3841 ccgtcacctt catccagcgg tcgccctcgc cgcggatctc gaagaccccg gacccggcca
     3901 gcacgaagcg cacctcgtcg tcgttgtgca ggtgctcgcc catgaacttc tggcagaggg
     3961 cgtcgaagtt gggcatgtcg ggccacatct tgacctggtc catggtcacg taacctcgtt
     4021 ccttcatcac ctgttccagc gggcccttgt aggcggtctc gtcggtgggg aagcgctcat
     4081 aggtcacgcc ctgggcgttg agctgctcgg gggaaaggga ctcgttggtg tccaaccaga
     4141 tggctttcat ggcgtctcct tgaactttga tcaagaccgg ttgcgtcccc cgggggggac
     4201 aggtgagggg tcagacccgg gtcgggtcga tccccagctg cttcatgcgg caggccgcct
     4261 cgaacaggta gtcgtagcat tcggcgtggg tcttggcctg cttccaatcc ttgccccaga
     4321 cgtagacgcc gtggcggcgc accagcacgg cgtgggactt ggggttgttg cggatggcct
     4381 cctccaacga gtcggccagg tcgcactcgt aggcggtgtt ctcgatgatg ggcacttcca
     4441 gggtgtcgaa ggcgccgaag ccctccaggc ccttttccat ctccatgttc ttgatgcgga
     4501 aatgcttgtc gaagagcagc gtcaccgaga ccgcgttgag cgagtggctg tgcagcacgg
     4561 cgccggcgtt gcggatcttg taggcgtgca taaagagggg ggaacattgg gagagtttca
     4621 gcccccgccc cggttcctcg gtgatggcgc cttccccgtc caggacgaag atgtccccgg
     4681 gggccaggcg ttccttctgg acgccgctgg gcgccatgta gatgcggccg tcttcc
//
LOCUS       DASTJJ010000255         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1382469, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000255 DASTJJ010000000
VERSION     DASTJJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382469"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="eno"
                     /locus_tag="VFR02_03120"
     CDS             <1..441
                     /gene="eno"
                     /locus_tag="VFR02_03120"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819785.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9869475.1"
                     /translation="IVTIEDGMAEDDWDGWKKLTDTLGDRVQLVGDDLFVTNVKRLSE
                     GISRHVANSILIKVNQIGTLTETLEAIEMAKRAGYTAIVSHRSGETEDTTIADIAVAT
                     NAGQIKTGSASRTDRIAKYNQLLRIEEELGASAQYLGKSVFYNL"
     gene            451..1371
                     /locus_tag="VFR02_03125"
     CDS             451..1371
                     /locus_tag="VFR02_03125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9869476.1"
                     /translation="MSQAALSGPRIYESVTELVGRTPLVEVRSHGVPGVKLYAKLESF
                     NPGGSVKDRPALRMIEQGIRSGKLTKDKVILDATSGNTGIAYAMIGAALGFKVLLAMP
                     ANVSDERKRILKSFNAEVRYSSPMEGSDGAIRLAREMLKEAPDKYYVPDQYNNPENPM
                     AHYLTTAEEILEAVGGEITHFAAGIGTSGTLMGTGKRLKEVKPSVQVVALEPAEPFHG
                     LEGLKHMASSIVPGIYDTHGYDFKITMDTEESYEWVQRLARREGLLVGLSSGGAMDGC
                     IKLAKRIQKGCIVTVFPDSGDKYLSTRVWE"
     gene            1374..1790
                     /locus_tag="VFR02_03130"
     CDS             1374..1790
                     /locus_tag="VFR02_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9869477.1"
                     /translation="MIRFEKQAEADMKGIGERGFPYEICGLMLGKSSPERLVTEVFEC
                     GNLNKLKPETRYDMDPKDYLKGETLARQKGLDVLGIFHSHPDHPDKASETDRQAAWPG
                     FSYVIMSIQKGKFASMRSWVLDEASQFQEEEISKTV"
     gene            1946..2359
                     /locus_tag="VFR02_03135"
     CDS             1946..2359
                     /locus_tag="VFR02_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9869478.1"
                     /translation="MKFYTEHLWFNTTKHREYINITGEVEKALKKSGIQEGMILVSAM
                     HITAGVYVNDAEDGLIADIDEWLEKLAPYKEDYRHHRTGETNGDSHLKSLLVHHEVIV
                     PVTAGKLDLGPWQQVYYAEFDGQRKKRLLIKVMGE"
     gene            2505..2801
                     /locus_tag="VFR02_03140"
     CDS             2505..2801
                     /locus_tag="VFR02_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9869479.1"
                     /translation="METSKPEFERYLLVCVNERPAGERVSCGGTFCGKQLAAALKDAV
                     KAAGQADKVRVSKTYCLDVCEEGANVLLYPDNIWFKQATLEDVPAILEKSGIKP"
BASE COUNT          643 a          873 c          922 g          519 t
ORIGIN      
        1 atcgtcacca tcgaagacgg catggccgag gacgactggg acgggtggaa gaagctcacc
       61 gacacgttgg gagaccgcgt gcagctggta ggcgacgacc tgttcgtcac caacgtgaaa
      121 cgcctctccg aaggcatcag ccgccatgtg gccaactcca tcctgatcaa ggtcaaccag
      181 atcggcaccc tgaccgagac cctggaggcc atcgagatgg ccaaacgggc cggctacacg
      241 gccatcgtgt cgcaccgctc gggcgagacc gaggacacca ccatcgcgga catcgcggtg
      301 gccaccaacg cgggccagat caagacgggc tcggcctccc gcacggaccg catcgccaag
      361 tacaaccagc tgctgcgcat cgaggaggag ctgggcgcgt cggcccagta cctgggcaag
      421 agcgtcttct acaacctgta agggggcgcg gtgagccagg cggccttgtc cggacccagg
      481 atctacgaga gcgtgaccga gctggtgggg cgcaccccgc tggtggaggt ccgctcgcac
      541 ggggtgccgg gggtcaagct ctacgccaag ctggagtcct tcaaccccgg cggctcggtc
      601 aaggaccggc ccgcgctgcg catgatcgag cagggcatcc gttcggggaa gctcacgaag
      661 gacaaggtca tcctggacgc cacctcgggc aacacgggca tcgcctacgc catgatcggc
      721 gcggccttgg ggttcaaggt gctcttggcc atgccggcca acgtcagcga cgagcgcaag
      781 cgcatcctga agtccttcaa cgcggaagtg cgttattcca gccccatgga gggctcggac
      841 ggcgccatcc gcctggcccg ggagatgttg aaggaagcgc cggacaagta ctatgtgccc
      901 gaccagtaca acaaccccga gaacccgatg gcgcattatc tcaccaccgc cgaggagatc
      961 ctggaggccg tgggcggcga gatcacccac ttcgccgcgg gcatcggcac cagcggcacc
     1021 ctgatgggca cgggcaagcg gctgaaggaa gtgaagccgt ccgtgcaggt ggtggccctg
     1081 gaaccggccg agcccttcca cggcctggag ggcctcaagc acatggcctc ctccatcgtg
     1141 ccgggcatct acgacaccca cggctacgat ttcaagatca ccatggacac cgaggaatcc
     1201 tacgagtggg tccagcgact ggcccggcgc gaggggctct tggtcgggct ttccagcggc
     1261 ggcgccatgg acggctgcat caagctggcg aagcggatcc aaaaaggctg catcgtgacg
     1321 gtcttccccg actcggggga taaatatctt tcgacccgcg tgtgggagtg accatgatcc
     1381 gtttcgagaa gcaggcggaa gccgacatga agggcatcgg ggaacggggg ttcccttatg
     1441 agatctgcgg cctgatgctg ggcaagtcct cgcccgagcg cctggtcacc gaggtcttcg
     1501 agtgcggcaa cttgaacaaa ctcaagcccg agacccgcta cgacatggac cccaaggact
     1561 atttgaaggg cgaaaccctg gcccgccaga agggcctgga cgtgctgggc atcttccatt
     1621 cccatcccga ccacccggac aaggcctccg agaccgaccg ccaagccgcc tggcccggct
     1681 tttcctacgt catcatgtcc atccagaagg gcaagttcgc ctccatgcgc tcctgggtgc
     1741 tggacgaggc ctcccaattc caagaagaag agatttccaa gactgtctga ccataaacga
     1801 ttttcaccac caagacacca aggcaccaag aaatgcgaaa tgatatttcg atttggttcc
     1861 cttggtgtct tggtgcctgc cccaacattg agtttttggt aacggggccg cctttgggcg
     1921 aggtggtgag gattggggta atgttttgaa gttttatacc gaacatcttt ggttcaacac
     1981 gaccaagcac cgggagtaca tcaacatcac gggcgaggtg gaaaaggcct tgaaaaagag
     2041 cggcatccag gagggcatga tcctggtgtc ggccatgcac atcaccgccg gggtctatgt
     2101 caacgacgcc gaggacggcc tcatcgccga catcgacgaa tggctggaga agctggcccc
     2161 ttataaagaa gactaccgcc accaccgcac cggcgagacc aacggggact cgcatttgaa
     2221 gagcctgctg gtgcatcatg aggtcatcgt gcccgtcacg gcggggaagc tggacctggg
     2281 cccctggcag caggtctatt acgccgaatt cgacggccaa cgcaagaaac gcctcctgat
     2341 caaggtgatg ggggaatagg caaaaacctt atttaccgca aagtcgccaa ggcgctaagt
     2401 gaagaaaggt cttaattctc aggttttgaa tcttgaacgt ggtttgaacg aagttcttgg
     2461 tgtcttggtg ccttggtggt gaagatggaa ttttggaggc gttcttggaa acgtctaaac
     2521 ccgaatttga gcgctatctg ctggtttgcg tgaacgaacg cccggcgggg gagcgagttt
     2581 cctgcggggg gaccttttgc ggcaaacaac tggccgccgc cctgaaggac gcggtcaagg
     2641 ccgcggggca agccgataag gtccgggtgt ccaagaccta ctgcctggat gtctgcgagg
     2701 aaggcgccaa cgtgctgctt tatcccgata atatctggtt caaacaggcc acgctggagg
     2761 atgtgcccgc catccttgag aaaagcggga tcaaaccgta agataaggcc gtcattgcga
     2821 ggcgtaagcc gaagcaatct cgggttgcgt cgttgtccag cccagtacag gctggacgaa
     2881 ctactataac tttgtgtcca ttggttttag ggactgaagc ccccggagcc tccacccagc
     2941 gagctgggtg gaggctc
//
LOCUS       DASTJJ010000256         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1627768, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000256 DASTJJ010000000
VERSION     DASTJJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627768"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFR02_03145"
     CDS             complement(<1..225)
                     /locus_tag="VFR02_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET9869480.1"
                     /translation="MAPLDTRTLPHEFPRRYVPAELDLGHWPSLEPLFGELEARALPS
                     AADLEKWLSDLLELEEVLDEEGSARYVRMTC"
     gene            297..1223
                     /locus_tag="VFR02_03150"
     CDS             297..1223
                     /locus_tag="VFR02_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9869481.1"
                     /translation="MTIYSYSRVNTYFTCPTQFEFRYVRKVPAPMPEGVELFLGSRFH
                     EAMEYLYGELPKRVPTVNEVMDFFKAHWDRKWRETLQKQQERGFATPLRIAQEGQTLE
                     DYFQKGQLYLENYYHKYQPFDQDATEGIEMKVLFDLDAEGRYKMQGFIDRLGRDAEGT
                     YWIHDYKTSARKLSEEDAKFEDQLALYQIGLSQNPKVGPRARVKLAWHFVAFDKDLVV
                     SERNAKETEWLKKKYVSKIQEIEKAKAFPAKPSRLCGWCEYLVLCGAGREYLERKGKK
                     VPEAPPPVAPSPAPGRPARGAKPSGTDQLSLF"
BASE COUNT          394 a          525 c          517 g          330 t
ORIGIN      
        1 gcaggtcatg cgcacgtagc gggccgagcc ttcctcgtcg aggacctcct ccagctccaa
       61 caggtccgac agccattttt ccaggtcggc ggccgacggc agcgcgcggg cctccagttc
      121 cccgaagagg ggctccaggg aaggccagtg gcccaggtcc agttcggcgg gaacgtagcg
      181 gcgggggaat tcgtggggga gcgtccgggt gtcgagaggg gccatacccc actttaccaa
      241 agcctgccgc caaaaaaaag ccgcgcgccc gcgcggggcc tggttataat ccgcccatga
      301 cgatctattc ctattcccgc gtcaacacct acttcacttg ccctacccag ttcgaattcc
      361 gttacgtccg caaggtcccg gcgcccatgc ctgagggcgt ggaacttttc ctggggtccc
      421 ggttccatga ggccatggaa tatctttacg gggaattgcc caagcgcgtg ccgaccgtca
      481 atgaggtgat ggatttcttc aaggcccatt gggaccggaa atggcgggaa accctccaaa
      541 aacagcagga acggggcttt gcgacccccc tgcgcatcgc ccaggaaggc cagaccctgg
      601 aggactattt tcaaaagggg cagctgtacc tggagaacta ttaccacaaa taccagcctt
      661 tcgaccagga cgccaccgag ggcatcgaga tgaaggtgtt gttcgacctg gacgcggaag
      721 gccgctacaa gatgcagggc ttcatcgacc ggttggggcg ggacgcggaa ggcacctatt
      781 ggatccacga ctataagacc agcgcccgca agctcagcga agaggacgcc aagttcgagg
      841 accagttggc cctctatcag atcgggcttt cccaaaaccc caaggtgggg ccccgggccc
      901 gcgtgaagct ggcttggcat tttgtggcct tcgacaagga cctggtggtc agcgagcgca
      961 acgccaagga gaccgagtgg ttgaagaaaa aatacgtctc caagatccag gagatcgaga
     1021 aggccaaggc cttccccgcc aagcccagcc ggctttgcgg ctggtgtgag tacctggtcc
     1081 tctgcggcgc cggccgggaa tacctggaaa ggaaggggaa aaaggtgccc gaggcgcccc
     1141 cacccgttgc gccttccccg gcgcccgggc ggcccgcccg gggggcgaaa ccttcgggga
     1201 cggatcaact ttctcttttt tgatgtgaca gaaaaacagc gaaaaaaacc tttggcgatt
     1261 attgatttcc ggggttttgg acggaaaaag aacgaaaatc agaaaatggg taaaaagtac
     1321 ccaattcaac cttcctttgt aatttaaaag cgaaccaagt tatgattccc ctggtttgac
     1381 ggttttttcc gaacccacac ccgctctttt cataccggat gagtgacaaa aagacagcga
     1441 aagaatcccc gacgtcggcc caatgggtcc cggggatata aacgttaaac ttctcccaaa
     1501 agcccgaaga gggggcgccc ctccccgggt tcccggaggc gtcgggcggt tcccgcggcg
     1561 tcgggtaggg tgaacgattc tggcagaagg gagaattcct tgtccgtcgt tttcggaaaa
     1621 gacaccttca tcgtttgccg caacagccag ggcgccgaga tccgggccac ttccctcagg
     1681 ctcacccgct acttggtcgt cttcgaggtc tacaacccct acagcatcct gcagttgtcc
     1741 gaagtgctcc tcgaattccg catctt
//
LOCUS       DASTJJ010000257         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1383522, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000257 DASTJJ010000000
VERSION     DASTJJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383522"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFR02_03155"
     CDS             complement(<1..285)
                     /locus_tag="VFR02_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9869482.1"
                     /translation="MAGDIAVPTPTNLIEVRGLTQDFPLPGGKTLRVLEKVDLGIHPR
                     EIVALLGPSGCGKSTLLRVLAGLIPPTRGEVFAQGLPLKGLNGGVSVVFQS"
     gene            complement(303..2030)
                     /locus_tag="VFR02_03160"
     CDS             complement(303..2030)
                     /locus_tag="VFR02_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9869483.1"
                     /translation="MIPLKKIWGEATSFLARYQKIWWIDLCLLLGFLGLVFGLSSVAG
                     EWTGRHRPAVVIDLSPAALPKYAFFSLCRGLLAFGLSLGFTLAYGYWAAKDRMAEKVL
                     IPLLDILQSIPVLGFMPGLVLALAALFPHSNVGLELAAVLMIFTGQVWNMAFSFYQSL
                     RSVPPALQEAAAVYGFGPLQKALKLEIPSAALGLIWNGMMSMAGGWFFLTVSESFQLG
                     GEDFRLPGLGSYMSVAAERGDHLAQLWGVTAMVAMIVALDQLLWRPLVVWGQKFRVEE
                     GGAEEAPRSWFLDFLRRSELLRLLRGLLAPKPAPPAASAAVPAPGRGGQGWLPVLSAL
                     CFLTLVGAMAFGAWKMAELFGRVTAAQWLLLAEEALATLGRVLLAVGLSALWTVPAGL
                     AIGLSPRLSRLVQPVVQVAASFPAPMLYAYVLLALGALGLSLNWGSVALMVLGAQWYL
                     LFNVVAGAMAIPSDLLEAARLYRLRGFALFRRVLLPGIFPYLVTGMVTAAGGTWNASI
                     VAEYVADRGHTLTAFGLGSQISRAAASADFPLLAAALVVMSLLVVAFNRLVWKPLYRL
                     AEEKYSLIK"
BASE COUNT          461 a          714 c          724 g          278 t
ORIGIN      
        1 actttggaac accacgctca cgcccccgtt gagacccttc aagggcaggc cctgggcgaa
       61 gacctccccc cgggtggggg ggatgagccc ggccaacacg cgaagcaagg tcgacttgcc
      121 gcagcccgag gggcccagca aggccacgat ctcgcgcggg tggatgccca ggtcgacctt
      181 ttccagcacc cgcagggtct ttcccccggg cagggggaag tcctgggtga ggccccgcac
      241 ttcgatgagg ttggttgggg tggggacggc gatgtcgccc gccatacgct ggtcctttca
      301 gcctatttga tcaaagaata cttttcctcg gccagccggt acagcggttt ccacaccaac
      361 cggttgaagg ccaccaccaa aagcgacatg accaccaggg cggcggccaa gagggggaag
      421 tcggccgagg cggccgcccg gctgatctgg gaacccagac cgaaggccgt cagggtgtgg
      481 ccccggtcgg cgacatattc ggccacgatg ctggcgttcc aggtgccgcc cgcggccgtc
      541 accatgcccg tgaccaggta gggaaaaatg cccggcaaaa gcacccggcg gaaaagggcg
      601 aagccccgca ggcggtagag gcgggccgcc tccaagaggt ccgaggggat ggccatggcc
      661 cccgccacca cgttgaacag caggtaccac tgggcgccca gcaccatcag ggccacgctg
      721 ccccagttga gggaaagccc caaggcgccc aaggccagca gcacgtaggc gtagagcatg
      781 ggcgcgggaa aggaggccgc cacctggacc accggttgga ccaggcggga aagccggggg
      841 gagaggccga tggccaaccc cgcgggcacg gtccaaagcg ccgagagccc cacagccaac
      901 aagacccggc ccagggtggc cagggcctcc tcggccaaga gcagccactg ggcggccgtg
      961 acccggccga aaagctccgc cattttccag gccccgaagg ccatggcgcc caccagggtg
     1021 aggaagcaga gggccgaaag gaccggcagc catccctggc cgccgcgccc cggggccggg
     1081 acagcggccg aggcggctgg cggggcgggt ttgggggcga gcagcccccg caaaagccgc
     1141 aacagctcgg aacggcgcag gaagtccagg aaccaggacc ggggggcctc ctcggcgccg
     1201 ccttcctcga cgcggaactt ctgtccccag accaccaggg gccgccaaag caactggtcc
     1261 agcgccacga tcatggccac catggccgtc acgccccaaa gctgggccag gtggtcaccc
     1321 cgctcggccg ccacgctcat gtaggacccc agcccgggca ggcggaagtc ctccccgccc
     1381 aactggaagg attcgctgac ggtcaggaag aaccagccgc ccgccatgct catcataccg
     1441 ttccaaatga gacccagggc cgccgaaggg atctccagct tcagggcctt ctgcaggggg
     1501 ccgaagccgt agaccgcggc ggcctcctgc agggcgggcg gcacggaccg aagcgactgg
     1561 taaaaactga aggccatgtt ccagacctgg ccggtgaaga tcatcagcac cgccgccagt
     1621 tccagcccga cgttgctgtg ggggaagagg gccgccaggg ccagcaccag gcccggcatg
     1681 aagcccaaca cggggatgct ctgcaggatg tccaacaggg ggatcaacac cttctcggcc
     1741 attcggtcct tggcggccca atagccgtag gccagggtga agcccagcga caggccgaag
     1801 gccaacaacc cccggcacag ggagaagaag gcgtatttgg gcagggccgc gggggacagg
     1861 tcgatgacca ccgcgggacg gtgccggccc gtccattcgc cggccacgga ggagaggccg
     1921 aagaccaggc ccaggaaccc caggagcagg cacaggtcga tccaccagat cttctggtaa
     1981 cgggccagga aggaggtggc ttccccccag atctttttga gtgggatcat ggcgcttccg
     2041 cgggcctcag gacccaattt ttttcggaac tatggctttt gcaaagcaag ccaaagagaa
     2101 cttgaaacaa tgataaaaac tgatgaaatc tgattttgaa aataaaaatc gggctttatc
     2161 agcttttatc agtgttc
//
LOCUS       DASTJJ010000258         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1750445, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000258 DASTJJ010000000
VERSION     DASTJJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750445"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFR02_03165"
     CDS             <1..536
                     /locus_tag="VFR02_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET9869484.1"
                     /translation="LAARFSLMERKKVTMVTADTYRIAATEQLKTYGRIMGIPVEVCG
                     SSEEIGDILNRHKNADIIFLDTAGRSPSNDDQLDELKRFILKSQADEIHLVISATTKY
                     FDMLKIIEKFGMAVPINRMIFTKLDETRHYGAFLNLMNNFQIPLSYCAVGQNVPDDLE
                     VPETRALSDRIVKALLS"
     gene            553..1458
                     /locus_tag="VFR02_03170"
     CDS             553..1458
                     /locus_tag="VFR02_03170"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET9869485.1"
                     /translation="MQDQAEKLRQLMGSLEPSPRTDRPALSGGAARVIAVTSGKGGVG
                     KSNISVNLALLMAQRGRKVLLVDADMGLANVDVLLGIIPRYTLVNVLNGQKKLAEIVT
                     EGPLGIRLVASGSGGVQELADLNEGQRNNFLQSLSDLQSDADVVLIDTGAGLHRNVLA
                     FVLAAQEVLIVTTPEPTALMDAYGMIKVAQREKKDPNLKVLVNMANNQLEADEAGKKL
                     VVLSKRFLNLDVQYLGYILRDLGVVKSVKEQKPLVLSNPLNPFAVHLGRVADHLLKPT
                     GREEDLNLIHFFKKVTRLFGGKDND"
     gene            1451..1870
                     /locus_tag="VFR02_03175"
     CDS             1451..1870
                     /locus_tag="VFR02_03175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9869486.1"
                     /translation="MTDNKKGQKRRFVRLNVVLPVKYRKYTGNPIFTSNFNVGRTIDL
                     SSGGLKLSVSRPIPAGSKLDMEIELDEKTASYVAGKVLGGEDKVIDGIGRRIEKITFV
                     DMDPEAQDLIMKFIFNNQRKQVRQGLKKPEVDHGKKR"
BASE COUNT          433 a          556 c          616 g          337 t
ORIGIN      
        1 agctggccgc gcgcttcagc ctgatggaac gtaaaaaagt gaccatggtg accgccgata
       61 cctaccgcat cgcggccacc gaacagctca agacctacgg gcgcatcatg ggcatcccgg
      121 tggaggtctg cggctcctcg gaggagatcg gggacatcct gaaccgccat aagaacgccg
      181 acatcatctt cctggacacc gcgggccgca gcccttccaa cgacgaccaa ttggacgagc
      241 ttaagcggtt catcctcaag agccaggccg acgagatcca tttggtgatc tcggccacca
      301 cgaaatactt cgacatgctc aagatcatcg aaaagttcgg gatggccgtg cccatcaacc
      361 ggatgatctt caccaaactg gatgagaccc gccattacgg ggccttcctc aacctgatga
      421 acaattttca gatcccgctt tcctattgcg cggtgggcca gaacgtgccg gacgacctgg
      481 aggtcccgga gacccgggcc ctgtcggacc gcatcgtgaa ggcattgctg agctgaaccc
      541 ctgggaggat acgtgcagga ccaagccgaa aagcttcgac aattgatggg cagccttgaa
      601 ccctcccccc ggacggaccg tcccgccttg agcggcgggg ccgcgcgggt gatcgcggtc
      661 accagcggca agggcggggt ggggaagtcc aacatctcgg tcaacttggc cctcctgatg
      721 gcccaacggg ggcgcaaggt gctgttggtg gacgccgaca tgggcttggc caacgtggac
      781 gtgttgttgg ggatcatccc ccgttacacc ctggtgaacg tgttgaacgg gcagaaaaaa
      841 ctcgccgaga tcgtcaccga gggcccgctg gggatccggc tggtcgcctc cgggtcgggg
      901 ggcgtgcagg aactggcgga cctgaacgag ggccagcgca ataacttcct gcagtccctc
      961 tcggacctgc agagcgacgc cgacgtcgta ttgatcgata cgggggcggg gctgcaccgc
     1021 aacgtgctgg ccttcgtgtt ggccgcccaa gaggtgttga tcgtgaccac gcccgagccc
     1081 acggccctca tggacgccta cgggatgatc aaggtcgccc agcgagaaaa gaaggacccg
     1141 aacctgaagg tcctcgtcaa catggccaac aaccaactgg aggcggacga ggcggggaag
     1201 aaactggtgg tgctttcgaa gcgtttcttg aacctggatg ttcagtacct ggggtacatc
     1261 ctgagggacc tgggggtggt caagagcgtg aaggaacaga agcccttggt gctctccaac
     1321 cccttgaatc ctttcgcggt gcacttggga cgcgtggccg atcatctgtt gaagcccacc
     1381 gggcgggaag aggatttgaa cttgatccat tttttcaaga aggtgacccg actcttcggc
     1441 ggtaaagaca atgactgaca acaaaaaagg ccagaaacgt cgttttgtcc ggttgaacgt
     1501 ggtgttgccc gtgaagtacc gcaaatacac ggggaacccc atcttcacca gcaatttcaa
     1561 cgtgggccgg accatcgacc tttccagcgg cgggctcaag ctgagcgtga gccggcccat
     1621 cccggcggga tccaagctgg acatggagat cgagttggac gagaagacgg cctcttatgt
     1681 ggcggggaag gtgctgggcg gcgaggacaa ggtcatcgac gggatcggcc gccggatcga
     1741 gaagatcacc ttcgtggaca tggatccgga ggcccaggac ctcatcatga agttcatttt
     1801 caacaaccaa cgcaagcagg tccggcaggg cctgaagaaa cccgaggtgg atcatggcaa
     1861 aaaaaggtaa gaacgcctac gaggtgacca aggaaaagcc cctgggttcc ctgggcctgc
     1921 tgatgacggg gggcgcggcg gg
//
LOCUS       DASTJJ010000259         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1384165, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000259 DASTJJ010000000
VERSION     DASTJJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384165"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1455
                     /locus_tag="VFR02_03180"
     CDS             220..1455
                     /locus_tag="VFR02_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HET9869487.1"
                     /translation="MKHQKNKHALAFIFITVLLDMLALGLIIPILPGLIVGLAGGDKV
                     RGALVVGLFGTVFALMQFVFSPVLGSLSDRFGRRPVILLSNLGMGLDYLVMALAPTLG
                     WLFVGRVLSGLTASSVGTAFAYIADSTTPKTRAAGFGVLGAAFSLGFVVGPGLGGWLG
                     GMGPRLPFWAAAGLSLVNFLYGFLVLPESLRASRRSAFQWKKANPVGSLQLLRSHAEL
                     SGLAWIYFLNSLAHGVFTSVFVLYVGYRYHWGPPLVGFCLMLVGLATGTVQGGLIRPV
                     VRRFGEKRALLAGLGFGLLGFLVFGWAPTGGLFNWGIPLSALWGLAGSPLQSLMSQRV
                     GRSIQGQLQGANGSLRAIAELIAPGLFTSIYAFFITPGRALQLPGSAFFLAAFLLVLA
                     GIGAWNVKTPGPGKTKEAR"
BASE COUNT          199 a          483 c          543 g          333 t
ORIGIN      
        1 caaacggtgg acccgaccta tgtggtgggg acccagttcc tcattcacta aggggaggac
       61 gtgaaggccc ggcccccagg ggccgggctt tttttttagg ggcctgggac cgcctgaaaa
      121 gggttaaaaa aaacgtccgg accctgtagg ctgggccggg cggagagagt tggaacgtcc
      181 taatccaagg tttcccccat cccatcgttc ggagacggat tgaaacacca aaagaacaaa
      241 cacgccctcg ccttcatctt catcaccgtg ctgctggaca tgttggccct ggggttgatc
      301 atccccatcc ttccgggcct gatcgtgggc ctggcggggg gcgacaaggt ccggggcgcc
      361 ctggtggtcg gccttttcgg gacggtcttc gccctgatgc agttcgtctt ctcgccggtc
      421 ctggggagcc tgtccgaccg tttcgggcga cgtccggtca tcctcttgtc caacctgggc
      481 atggggttgg attacctggt catggccctg gcccccaccc tgggctggct cttcgtgggg
      541 cgggtgctgt cgggcttgac cgcttccagc gtgggcacgg ccttcgccta tatcgccgat
      601 tccaccaccc ccaagacccg cgccgcgggg ttcggcgtcc tgggcgcggc cttcagcctg
      661 gggttcgtgg tgggcccggg cctgggcgga tggctggggg ggatgggccc gcgcctgccc
      721 ttttgggcgg cggcgggatt gagcctggtc aacttcctct acggcttcct cgtcctgccc
      781 gagtccctga gggcgtcccg gcgctcggcc ttccaatgga aaaaggcgaa cccggtgggg
      841 tcgctccaac tgctccgttc ccacgccgaa ttgtccgggt tggcctggat ctatttcctc
      901 aacagcctgg cgcacggggt cttcaccagc gtgttcgtgc tttacgtggg ctaccgctac
      961 cattggggcc cgcccctggt gggcttttgc ctgatgctgg tggggctggc cacggggacc
     1021 gtccagggcg ggctgatccg gcccgtggtg aggcgcttcg gggaaaagcg ggcgctgttg
     1081 gcggggttgg gcttcggcct gctggggttc ctggtgttcg gctgggcccc caccggcggc
     1141 ctcttcaatt ggggcatccc cttgagcgcg ctttggggct tggccgggtc gcccctgcag
     1201 agcctcatgt cccaacgggt cggccggtcc atccagggcc aattgcaggg ggccaacggt
     1261 tccctccggg ccatcgccga actgatcgcc cccggccttt tcacctccat ctacgccttc
     1321 ttcatcaccc ccggccgggc cctccaactg ccgggttcgg ccttttttct ggcggctttt
     1381 ttgctggtgt tggccgggat cggggcctgg aacgtcaaaa ccccgggtcc gggaaagacc
     1441 aaggaggcgc gatgagtcac tggatatggg tgatggtggc gggggtcttg gtgggatggc
     1501 tggcgggcct attggtcggg ggcaaggggt tgggcctctt gggcaacatg gtggtggg
//
LOCUS       DASTJJ010000260         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1628989, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000260 DASTJJ010000000
VERSION     DASTJJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628989"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFR02_03185"
     CDS             <1..192
                     /locus_tag="VFR02_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET9869488.1"
                     /translation="GLRPYFAVMAFILFRLFDIWKPYPIRRIENLPGGWGIMTDDLLA
                     GLYANFFLQVGALVVRVIA"
     gene            206..1480
                     /locus_tag="VFR02_03190"
     CDS             206..1480
                     /locus_tag="VFR02_03190"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9869489.1"
                     /translation="MNAEIVAIGTELLLGQSRDTNSSWLAGELARLGVDLFGFQAVGD
                     NEKRLEAALKGALDRAAVVLTTGGLGPTVDDVTRKVAGRIARRQLVFHEDIAKALEDR
                     FTRLRPGKPFPKVNLNQAFIPQGALVIPNPVGTAPGFIVKADKSHLICLPGVPAEMKA
                     MFEATVKPFLKSLAPGGALIKSRVYRTTGLFESALNERIADLFENSTNPTIGVLAHLE
                     GVDIRLTAKADTEAQADALLDGLSKALTLRLPTHLYGLDQDGLETIVGKILTTRSLTL
                     ATAESCTGGLIAHRVTLVPGSSRYFLRGAVVYSNEAKTDLFQVDPKLIEAKGAVSAEV
                     AEVLARQCRLRAGADLGLSTTGIAGPTGGSAEKPVGLVFIALADEQSVQVFRHQFSGD
                     RAEVKFRASQAALELVRRYCLGLPLRDGDEVR"
     gene            1477..>1984
                     /gene="thpR"
                     /locus_tag="VFR02_03195"
     CDS             1477..>1984
                     /gene="thpR"
                     /locus_tag="VFR02_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9869490.1"
                     /translation="MNTLRLFAALDLPAGIRESWKGILDGDALPQGVRWVRPGDLHVT
                     LRFFGELGADKEPGVGDLLAKAAARHRPFSISLRGLGAFPPAGKTRVVFLGVEEGRQA
                     LAALAQEIAKSAGLFGWGTPGEFQAHVTLGRVGNADSARVPDLLRAWAAGGFGPWKVD
                     AFSLYQSRL"
BASE COUNT          300 a          649 c          691 g          344 t
ORIGIN      
        1 ggcctgcggc cctacttcgc cgtgatggcc ttcatccttt tccgcctttt cgacatctgg
       61 aagccctatc ccatccgccg catcgagaac ctgccgggcg gttggggcat catgaccgac
      121 gacctgctgg cgggcctcta cgccaacttc ttcctgcagg tgggcgccct ggtggttcgg
      181 gtgatcgcct gatcccggag gccgcatgaa cgctgagatc gtcgccatcg gcaccgaact
      241 gctgttgggc caaagccggg acaccaactc atcctggttg gcgggggaat tggcccgcct
      301 gggcgtggac ctgttcggct tccaggccgt gggggacaat gaaaaacgcc tggaagcggc
      361 cctgaagggg gccctggacc gggccgcggt ggtcctgacc acgggggggt tgggacccac
      421 ggtggacgac gtgacccgca aggtggcggg gcgcatcgcc cggcgccaac tggtcttcca
      481 tgaggacatc gccaaggccc tggaggaccg gttcacccgc ttgcgcccgg gcaaaccctt
      541 tcccaaggtg aacctgaacc aggccttcat cccccagggg gccctggtca tccccaaccc
      601 ggtgggcacg gcgccggggt tcatcgtgaa ggccgacaag tcgcacctga tctgcctgcc
      661 cggcgtgccc gccgagatga aggccatgtt cgaggcgacg gtgaagcctt tcttgaagtc
      721 cctggcgccg gggggcgcgc tcatcaagtc ccgggtctac cgcaccaccg gattgttcga
      781 gtcggccctg aatgaacgga tcgccgacct gttcgagaat tccaccaacc cgaccatcgg
      841 cgttctcgcc cacctggagg gcgtggacat ccgtctgacc gccaaggccg acaccgaagc
      901 ccaggccgac gccttgctgg acggcctgag caaggccctc acgctccgcc ttcccaccca
      961 tctttacggc ctggatcagg acgggttgga gaccattgtg ggcaagatcc tcaccacccg
     1021 gtccctcacc ctggccaccg ccgagtcctg cacgggcggg ctgatcgccc atcgggtcac
     1081 cctggtgccc gggagttccc gttatttcct gcggggggcg gtggtctaca gcaacgaggc
     1141 caagacggac ctcttccagg tggatcccaa gttgatcgag gccaagggcg cggtcagcgc
     1201 cgaggtggcg gaagtcctgg cccgccaatg ccgtctccgg gcgggggcgg acctgggctt
     1261 gtccaccacg ggcatcgcgg gccccacggg cggctcggcg gaaaaaccgg tggggctggt
     1321 cttcatcgcg ctggccgacg aacaatcggt ccaggtgttc cgtcatcaat tttcggggga
     1381 tcgggccgag gtgaagttcc gggccagcca agcggccttg gagctggtcc gccgttactg
     1441 cctgggactg cccttgcgcg acggggacga ggtccggtga acaccctccg gttgttcgcg
     1501 gcgctcgacc tgccggccgg gatccgggaa agttggaaag gtattttgga cggggacgcc
     1561 cttccccaag gggtccgctg ggtgaggccc ggggacctgc atgtgaccct gcgttttttc
     1621 ggcgaattgg gcgccgacaa ggagcccggg gtgggggacc tgctggccaa ggccgcggcc
     1681 cggcacaggc ccttttccat ttccttgcgg gggctggggg cattcccccc ggcggggaag
     1741 acccgggtgg tcttcctggg ggtggaggag ggcaggcagg ccttggcggc tttggcgcaa
     1801 gagatcgcaa aaagcgccgg cttgttcggc tgggggactc ccggggagtt ccaggcccat
     1861 gtgaccctgg gccgggtggg aaacgccgac tccgcccggg tcccggatct cttgcgggcc
     1921 tgggcggcgg ggggcttcgg tccctggaag gtggacgcct tctcgcttta ccaaagccgc
     1981 ctgg
//
LOCUS       DASTJJ010000261         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_162232, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000261 DASTJJ010000000
VERSION     DASTJJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162232"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1003
                     /locus_tag="VFR02_03200"
     CDS             218..1003
                     /locus_tag="VFR02_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9869491.1"
                     /translation="MLFKIEQLKGYQLQSFDGKIGGVKEFYFDDHHWTVRYLVADTGR
                     WLTDRQVLISPYALAGVNGPEKTISVALTRKQIEESPSLDSDKPVSRQFEEEYHGFYG
                     WPMYWGGPYMWGAYPGLMRDREKQAEPPPARRAWDPHLCSSRSVTGHTVQASDGEIGH
                     VDDFILDDETWAVRYLVVDTGNWWPGKKVLVSTRWIESVGWDRSKVFVGLTRETIQQS
                     PEYDGESLVVRDYEAELHRHYNRRGYWEEELAAQKRSAEPRTP"
     gene            1032..>1289
                     /locus_tag="VFR02_03205"
     CDS             1032..>1289
                     /locus_tag="VFR02_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869492.1"
                     /translation="MKKTIVCVFLAGWIPMLALTGCANNGGGTGPSAHLYPFQKPQAM
                     VSLGSAASYGILAYSAITNSGPTTLCGSLGIAPLSSVSGGIV"
BASE COUNT          258 a          361 c          432 g          238 t
ORIGIN      
        1 cccatcaaaa taagagcgcg gctatcaatt tgagggcctg gccgccggat ggaagggcgg
       61 gtcggggaca ggagcggttt ttttgcccgt aagcgttgat ccgggacttc caggcgcggg
      121 gcatggtcct tgctttgcct atgtccgatc gcgttcggag ggtggtgccg cattttggga
      181 agcccgccaa gaccggctcg ggaagggtgg aaagtccatg ctgttcaaga tcgaacaatt
      241 gaagggctat caactgcaaa gtttcgacgg aaagatcggc ggggtgaagg aattctattt
      301 tgacgaccac cattggacgg tccgctacct ggtggccgac acgggccgtt ggttgacgga
      361 ccggcaggtg ctgatctcac cctacgccct ggccggcgtg aatgggccgg aaaagaccat
      421 ttcggtggcc ttgacccgaa agcagatcga ggagagccct tccttggaca gcgacaagcc
      481 ggtatcgagg caattcgagg aggaatacca cggcttttac ggttggccca tgtattgggg
      541 cggtccctat atgtggggcg cctacccggg cctgatgcgg gaccgggaaa aacaggcgga
      601 gcccccgccg gcccggcggg cttgggaccc ccacctgtgc agcagccgtt cggtgaccgg
      661 ccacaccgtc caagcctccg acggcgagat cggccatgtg gatgatttca tcctggatga
      721 cgagacctgg gccgtgcggt acctggtggt cgacacggga aattggtggc cgggcaaaaa
      781 ggtcctggtg tcgacccgat ggatcgaaag cgtgggctgg gaccggtcga aagtgttcgt
      841 gggcctgacc cgggagacca tccaacaatc ccccgaatat gacggggagt ccctggtggt
      901 ccgggactac gaggccgaac tccaccggca ctacaaccgc cggggttatt gggaggaaga
      961 actggccgcc caaaaacgtt ccgctgagcc gcgcaccccg tgacacgctt aaaatccaaa
     1021 ggagatgaaa agtgaaaaaa acgatcgttt gcgtcttttt ggccggatgg atcccgatgt
     1081 tggcgctgac gggatgcgcg aataacggcg gtgggacggg tccctcggcc catctttatc
     1141 cattccagaa gccccaggcg atggtgtcgc tcgggtcggc cgccagctac ggcatcctgg
     1201 cctattcggc gatcacgaac tccggcccga ccaccttgtg cgggagcctg ggcatcgcgc
     1261 ccttgagctc ggtgagcggc ggcatcgtg
//
LOCUS       DASTJJ010000262         1482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1384570, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000262 DASTJJ010000000
VERSION     DASTJJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384570"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1154)
                     /locus_tag="VFR02_03210"
     CDS             complement(30..1154)
                     /locus_tag="VFR02_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9869493.1"
                     /translation="MTTYPAQAYAADGRGGARDCRLSLGPSGLSGETGDGLSFQWAYR
                     DLRLHHTGEDGGFLRLKSAAPGAFDGSLITRDDAFREALAARLGEPHRSFLLNFKKQH
                     RGILAAKWRNLALAALGAAALFLGGYWALGHWAADFAAKHLPIPVETAWGEVQARSEM
                     AQGQVVEGGPAVAAVQTIVDRLAAAVPDNPGYHFQVHVLESPEVNAFALPGGQIFVLT
                     GLLGEAKDPEEVAGVLAHEMQHVLHRHVVRRTVEELGFRLWVALLFGHTGLAVLAQQA
                     GKVMDLSFSRSQESQADLDGARLLARADLPVAPLEDFFTRLAEQQGLMGAKAVALLED
                     HPASLDRVAALRALAQKIEPKHPRDFPGIDWKAVQASLKN"
     gene            complement(1183..>1482)
                     /locus_tag="VFR02_03215"
     CDS             complement(1183..>1482)
                     /locus_tag="VFR02_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869494.1"
                     /translation="GDWTADSAGMGYGGGALFFSLLLAGVAVLYYRTRVSRSALFWAA
                     FILTRPLGAVVGDFLDKPVAHGGLALSRYAASALLAAFIAVCILVFPHRAAKRSH"
BASE COUNT          258 a          517 c          505 g          202 t
ORIGIN      
        1 tctttgcggt gaaaaagccg ttggatcccc taatttttca gcgaagcctg gacggccttc
       61 caatcgatgc ccgggaaatc cctgggatgt ttgggttcga tcttttgggc cagtgcccgc
      121 agggccgcca cgcggtccag gctggccgga tggtcttcca agagggccac ggccttggcg
      181 cccatcaggc cctgctgttc ggccagccgg gtgaaaaaat cctccagggg cgccaccggc
      241 agatccgccc gggccagcag cctggccccg tccaggtcgg cctgggattc ctgcgagcgg
      301 ctgaaggaaa ggtccatcac cttgcccgcc tgttgggcca ggaccgccag gcccgtatgg
      361 ccgaagagca gggccaccca aagccggaag cccaattctt ccaccgtgcg gcgcaccacg
      421 tgccggtgca gcacatgctg catctcgtgg gccaggacgc ccgccacctc ctcaggatcc
      481 ttggcctccc ccaaaagccc ggtcagcacg aagatctggc ccccgggcag ggcgaaggcg
      541 ttcacttccg ggctttccag cacgtggacc tggaaatgat agcccgggtt gtcgggcacg
      601 gccgccgcca ggcggtccac gatggtctgg acggccgcca ccgccgggcc cccttccacg
      661 acctgtccct gcgccatttc ggaccgggcc tggacctcgc cccaggcggt ctccacgggg
      721 atcggcaggt gcttggcggc gaaatccgcc gcccaatggc ccaaggccca atagccgccc
      781 aggaagaggg ccgccgcccc caaggcggcc aaggccaggt tgcgccactt ggcggcgagg
      841 atgccgcggt gctgcttctt gaagttcaaa aggaaggaac ggtggggttc gcccaggcgc
      901 gcggccaggg cctcccggaa agcgtcgtcc cgcgtgatga ggcttccgtc gaaggccccg
      961 ggggccgcgc tcttgaggcg caggaacccg ccgtcctcgc ccgtgtggtg cagtcgcagg
     1021 tcccgatagg cccactggaa ggaaagcccg tccccggtct cgcccgaaag gcccgaggga
     1081 cccagcgaca gccggcaatc ccgggcgccg ccccgtccgt cggcggcata ggcttgggcg
     1141 ggataggtgg tcaccgggat cccctttccg aacgcccggg ggtcaatggg accgcttggc
     1201 cgcgcggtgc gggaagacga ggatgcagac cgcgatgaag gcggccaaga gggcggaggc
     1261 ggcgtagcgg ctcaaggcca ggcccccgtg ggccacgggc ttgtcgagga aatcccccac
     1321 cacggcgccc aagggccggg tcaggatgaa ggccgcccaa aagagggcgg accgggaaac
     1381 gcgggtccgg taataaagga ccgccacccc ggccaacagc aacgagaaga acagggcgcc
     1441 tccgccgtaa cccatgcccg ccgaatccgc ggtccagtcg cc
//
LOCUS       DASTJJ010000263         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1507042, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000263 DASTJJ010000000
VERSION     DASTJJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507042"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..2112)
                     /locus_tag="VFR02_03220"
     CDS             complement(301..2112)
                     /locus_tag="VFR02_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9869495.1"
                     /translation="MRYQPIENYGIIGDLHTAALVGMDGSIDFLCFPRFDSPPVFNAL
                     LDAPKGGRFQISPVLEGARQKQLYLSDTNILLTRFLSDDGVAEISDYMPVAEVGHAHQ
                     LVRRVKTVRGKVRFRMTCDPRFDYGRDPVPARAQGKSVVWEPKSGPVKSLRLQSAVPL
                     TLSEGKASAEFELEAGGKAHFVLGETDPARAPFPLEEKFLTENFKRTVDYWRAWVSRS
                     QYKGRWREMVNRSALTLKLLTSSADGSMVAAPTFGLPEEIGGVRNWDYRYTWVRDSSF
                     TLYGLMRLGYVEEARAYMRWIEARLAAPLPDGSLQIMYGLQGEKELPERELAGWDGYR
                     GSRPVRVGNAASGQLQLDIYGELMDSVYLFDRHGETLSHDLWADLVRVVDWVCANWER
                     PDDGVWEVRGGRQEFLYSRFMCWVAVDRAVRLSQKRSYPAPLTRWQETRDRIYRDIFE
                     HFWDKDLGAFVQHRGAKRVDASTLLMPLVKFISPADPRWLSTLKVIGKDLVSDSLVYR
                     YRHDGAEGDGLSGREGTFSMCTFWYVECLSRAGDLDRARFFFEKMLGYANHLGLYSEE
                     LGPKGEHLGNFPQAFTHLALISAAFDLDRRLTQAGKG"
     gene            complement(2233..>2564)
                     /locus_tag="VFR02_03225"
     CDS             complement(2233..>2564)
                     /locus_tag="VFR02_03225"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9869496.1"
                     /translation="CFAPNDGGFHPLTALGAGWGCFTLVSLVGLPPWLLLGWLVSRNA
                     SFEPAWTGAWTGASAFLLGTTTVQLHCPDNQWGHVLMAHLLPVALFTVAASWAGLYWF
                     SRWKKKA"
BASE COUNT          472 a          861 c          835 g          396 t
ORIGIN      
        1 ccggtcaatt tcgccctgac ctcgcgcgag ttcctgcccg aggggctcaa gaagaagctg
       61 gcgggccccc gcgcccgctg gtccgccatg cgctaccagc tctataaact gtaagccccc
      121 ctccgaaact ttcaaaagaa aagccctttc accgcaaaga cgcggggaaa atacgctcat
      181 ctttcggctt ttaacgacca cctcgacttt tcgtcccgac gacaaaggca aatatattta
      241 tcgcttatgg tcgacggcct tcctcaactt tgcgcattgg cgtctttgcg gtgaaaaatt
      301 tcatcccttc cccgcctggg tcagccggcg gtccaggtcg aaggccgcgc tgatcaaggc
      361 caggtgggtg aaggcctggg ggaaattgcc caggtgctcg cccttgggcc ccagttcctc
      421 cgaataaagc cccaggtggt tggcgtagcc cagcatcttc tcgaaaaaga accgggcccg
      481 gtccaggtcg ccggcgcgcg acaggcactc cacgtaccag aaggtgcaca tgctgaaggt
      541 gccctcccgc ccgctcaagc cgtcgccttc cgccccgtca tgccggtacc ggtagaccaa
      601 tgaatcgctc accaggtcct tcccgatcac cttcagggtc gagagccagc gggggtccgc
      661 cgggctgatg aacttgacca ggggcatcaa gagcgtggag gcgtccaccc gcttggcccc
      721 ccggtgctgg acgaaggccc ccaggtcctt gtcccagaaa tgctcgaaga tgtcgcggta
      781 gatgcggtcg cgcgtctcct gccagcgggt caggggcgcc gggtaggagc gtttttggga
      841 cagccgcacg gcccggtcca ccgccaccca gcacatgaaa cgggagtaaa ggaattcctg
      901 gcgcccgccg cgcacctccc agacgccgtc gtccgggcgc tcccaattgg cgcagaccca
      961 gtccaccacc cgcaccaggt ccgcccagag gtcgtgcgac agggtctcgc cgtgccggtc
     1021 gaagaggtag accgaatcca tcagttcccc gtagatgtcc agctgcagtt ggccggaggc
     1081 cgcgttcccc acccgcacgg gccgggaccc ccggtagccg tcccaaccgg ccagttcccg
     1141 ctcgggcagt tccttctcgc cctgcaggcc gtacatgatc tggagcgacc cgtcgggcag
     1201 gggcgcggcc aaccgggcct cgatccagcg catgtaggcc cgggcctcct ccacgtaacc
     1261 caaccgcatg agcccgtaga gggtgaagga ggagtcgcgg acccaggtgt agcggtagtc
     1321 ccagttgcgc acccccccga tctcttccgg caaaccgaag gtgggggccg ccaccatgga
     1381 gccgtcggcg ctggacgtga gcagtttgag cgtgagggcg gaccggttga ccatttcccg
     1441 ccagcggccc ttgtattggg accggctgac ccacgcgcgc cagtagtcga ccgttcgctt
     1501 gaagttctcc gtcaggaact tttcctccag cgggaagggc gcccgggccg ggtcggtctc
     1561 gcccagcacg aaatgggcct tgccgcccgc ctccaactcg aattcggccg aggctttgcc
     1621 ctcggacagg gtgaggggca cggcgctctg cagccgaagg gacttcaccg gacccgactt
     1681 gggttcccag accacggact tcccctgggc ccgcgccggg accgggtccc ggccatagtc
     1741 gaaccgggga tcgcaggtca tccggaagcg cactttcccc cgcacggtct tgacccgccg
     1801 caccagttgg tgggcgtggc ccacctcggc caccggcatg tagtccgaga tctcggccac
     1861 gccgtcgtcc gacaggaacc gggtcagcag gatgttggtg tccgaaaggt aaagctgctt
     1921 ctggcgggcg ccttccagca cgggcgagat ctggaaccgc ccccccttcg gggcgtccag
     1981 gagggcgttg aagaccgggg gcgagtcgaa ccgggggaaa cagaggaaat cgatggaacc
     2041 gtccatcccc accagggcgg cggtatggag gtcgccgatg atgccgtaat tctcgatggg
     2101 ttggtaacgc atggggctcc ttgggcggga aatggcctca ggagttagga cgccgccggg
     2161 gggcggcagg cgtcaccgcg agggcgaaaa agcgggggcc cggggacggg aaagaccccg
     2221 ggcccaaaag gatcaggcct tctttttcca gcgggagaac caataaagcc ccgcccagct
     2281 ggcggccacg gtgaagagcg ccaccggcag cagatgggcc atcagcacat ggccccattg
     2341 gttgtcgggg cagtgcagct gcaccgtggt ggtgcccaag aggaaggccg aagcgccggt
     2401 ccaggcgccg gtccaggccg gctcgaagga ggcgttgcgg gacaccagcc agcccagcag
     2461 cagccaaggc ggcaggccca ccagggagac caaggtgaaa cagccccagc ccgcgcccag
     2521 ggcggtcagg ggatggaacc cgccgtcgtt gggcgcgaaa caga
//
LOCUS       DASTJJ010000264         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1507064, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000264 DASTJJ010000000
VERSION     DASTJJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507064"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFR02_03230"
     CDS             <1..712
                     /locus_tag="VFR02_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9869497.1"
                     /translation="GLYAVVTTVNQAKADTTTFTAGQAHEGEKLFVANCATCHGLGAK
                     GTENGPSLAGVGAAAVSFQVGTGRMPMQMQGPQALQKPAQFNADQTNELAAYVATLGN
                     GPGIPEAQYLDGKGDATKGGELFRVNCAMCHNAAAAGGALTRGKFAPALAGVSNDHIY
                     EAMVTGPQNMPVFNDANITPEGKRDIITFLNTIEHQGSPGGAALGSLGPVSEGLFLWT
                     AVLGVVIGFTVWLTSRTS"
     gene            809..>1130
                     /locus_tag="VFR02_03235"
     CDS             809..>1130
                     /locus_tag="VFR02_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C reductase"
                     /protein_id="HET9869498.1"
                     /translation="MGNHSDGTPASSGAVAKAGHEEVEKFQDPGLPPHRLRLADTDPK
                     AAKRAERQVAILFGISVIGTLLFFFAYFGIRLDQTLTTLHLQNMFLGLGVGFAMLGIG
                     TGIVH"
BASE COUNT          219 a          386 c          340 g          185 t
ORIGIN      
        1 tgggctgtac gccgttgtca caacagtgaa ccaggccaag gctgacacca ccacgtttac
       61 cgccggccag gcccacgagg gcgagaagct gtttgtggcc aactgcgcca cctgccacgg
      121 cctcggtgcc aagggcacgg aaaacggccc ctccctggca ggcgtcggcg ctgcggcagt
      181 cagcttccag gtcggcacgg gccgcatgcc catgcagatg cagggcccgc aggccctcca
      241 gaagccggcc cagttcaacg ccgaccagac caacgaactc gcagcctacg tcgccaccct
      301 cggcaacggc ccgggcatcc ccgaagcgca gtaccttgac ggcaagggag acgccaccaa
      361 gggtggcgag ctgttccgcg tcaactgcgc catgtgccac aacgctgcag ctgccggcgg
      421 cgccctgacc cgcggcaagt ttgcaccggc cctggccggc gtgtccaacg accacattta
      481 cgaagcgatg gtcaccggtc cgcagaacat gccggtcttc aacgacgcaa acatcacccc
      541 ggaaggcaag cgggacatca tcacgttcct gaacaccatc gagcaccagg gctcccccgg
      601 tggagccgct ctcggctccc ttggcccggt ctccgaaggc ctgttcctgt ggacggccgt
      661 cctcggcgtg gtcatcggct tcacggtctg gctgacgtcc aggacttcct gaccggcctc
      721 cacaagattt tggctgcccc aggcagccgc gcgaaacata cattacgacc ccgggaaagt
      781 accgggataa gagaaggatg aggggattat gggcaaccat agtgacggca cgccggccag
      841 ttcgggcgcc gtagctaagg ctggtcacga agaggtggag aagttccagg acccgggact
      901 tccgccacat cgtttgcgac tggctgacac ggaccccaag gcagcaaagc gggccgagcg
      961 ccaggtggca atcctgtttg ggatttccgt catcggcacc ctgctcttct tctttgccta
     1021 ctttggcatt cgcctggacc agacactgac caccctgcac ctccagaaca tgttcctggg
     1081 cctgggcgtc ggtttcgcca tgctgggcat tggaacgggc atcgtgcatt
//
LOCUS       DASTJJ010000265         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_284257, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000265 DASTJJ010000000
VERSION     DASTJJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284257"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..390
                     /locus_tag="VFR02_03240"
     CDS             106..390
                     /locus_tag="VFR02_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869499.1"
                     /translation="MKAFRKISFCLALAGFSAFLTLSLAENFHHHGALESHDHCAMCA
                     WTQADAKAVPVTLPHLLAALVLVFVAPRVFHRAPAPRLIPLPGRSPPTLP"
     gene            430..1524
                     /locus_tag="VFR02_03245"
     CDS             430..1524
                     /locus_tag="VFR02_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869500.1"
                     /translation="MRIHRFLLLGTALVGLSAGGAQAQTRSIISNLANPAIGMSALFL
                     AQAAPDLSVPGTTGPAFQEAELSVISTVDPTWTLTGNFTFDAESGLAEEAFATTDSVP
                     NVLLKVGEIRAAFGKHGTLHTHAFPFIQAPIVMANTIGEEGFKDAGIEAAWMTPLPWY
                     CELTLGVYHPTAASADHNLNFNSASLDNLPWLLHLKDLVDLDDDTTLELGGSALSGMG
                     DDGLHHAVYGADLTVRNIPLRQSNQRGWILQGEYLRKVSFADGTYNQGSDGWYASFQY
                     RLAQEWWTGVRAEEAFHVTPDDLTQGITENPLSGHVQRASANVTWQGSEFSALRLEYS
                     LAKSADSQQTDNRVMLQFNYVIGFHPPHAY"
     gene            1567..>1952
                     /locus_tag="VFR02_03250"
     CDS             1567..>1952
                     /locus_tag="VFR02_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET9869501.1"
                     /translation="MKKVNILLSALLAWGLSLPAFADLKVATALSDLASVAQFVGGDH
                     VTAMSLCKGYEDPHFVPAKPSLMKAIQDAGVFVSVGLELDGGWLPLVIPGSRNPKIQP
                     GQSGFVDASEGIDVLDKPVGTVSRAEG"
BASE COUNT          332 a          689 c          584 g          347 t
ORIGIN      
        1 cggcgcgtcg cccattcatt ttatcccaaa tcgggccggg cgttgacaaa gttcgggacc
       61 gctcccaata atgtggacgt tcagcgatcc cgtgaaggac tttccatgaa ggcttttcga
      121 aaaatttcct tctgcctcgc cctggcgggc ttctcggcct tcctcaccct gtccttggcc
      181 gagaacttcc accaccacgg cgcgctggaa agccacgacc attgcgccat gtgcgcctgg
      241 acccaggccg atgccaaggc cgtcccggtc accctgcccc atctgctggc cgcgctggtc
      301 ctggtgttcg tcgccccgcg ggtcttccac cgcgccccgg cgccccgcct gatcccgctg
      361 cctggaaggt cccctcccac cctcccgtaa ttttttcgcg ccgtccgaaa aaacatacgg
      421 gaggaattca tgcgaattca tcgtttttta ttgttgggga ccgcccttgt ggggctgtcc
      481 gccggggggg cccaagccca gacccggtcg atcatttcca acctggccaa tcccgccatc
      541 gggatgagcg cccttttcct ggcccaggcc gcgcccgacc tgtcggtgcc cggcaccacc
      601 ggtccggcct tccaggaagc cgagctgagc gtcatttcca cggtggaccc cacctggacg
      661 ctcacgggca acttcacctt cgacgccgaa agcggattgg ccgaggaggc tttcgccacc
      721 acggacagcg tccccaacgt cctgttgaag gtcggcgaga tccgtgcggc cttcggcaag
      781 cacggcacgc tgcacaccca cgccttcccc ttcatccagg cgcccatcgt gatggccaac
      841 accatcggcg aggagggctt caaggacgcc ggcatcgagg cggcctggat gacgccgctg
      901 ccttggtact gcgagctgac cctgggggtc taccacccca cggcggcctc ggcggaccac
      961 aacctcaact tcaacagcgc ctccctggac aacctcccct ggctgctcca cctgaaggac
     1021 ctggtggacc tggacgacga caccaccctg gaactgggcg ggtcggcgct ctcgggcatg
     1081 ggggacgacg ggctgcacca cgcggtctac ggggcggacc tgacggtgcg caacatcccc
     1141 ctgcgccagt cgaaccagcg gggctggatc ctgcagggcg agtacctgcg caaggtctcc
     1201 ttcgcggacg ggacctataa ccaggggtcc gacggttggt acgcgtcctt ccaataccgg
     1261 ctggcccagg aatggtggac gggcgtgcgg gccgaggagg ccttccacgt cacgccggac
     1321 gacctgacgc agggcatcac cgagaacccg ctttccggcc atgtccaacg ggcctcggcc
     1381 aacgtcacct ggcaaggctc cgagttctcg gcccttcgcc tggaatacag cctggccaag
     1441 tcggcggact cacagcagac cgacaaccgg gtcatgttgc agttcaacta cgtgatcgga
     1501 ttccacccgc cgcacgccta ttaaaccgcg ccattgggac tttaaaacct gttcaaggag
     1561 atcgaaatga aaaaagtgaa tatcctgtta tccgcgttgt tggcctgggg gctttcgctc
     1621 ccggccttcg ccgacctgaa ggtggccacc gccctctccg acctggcctc ggtggcccag
     1681 ttcgtcgggg gggaccatgt caccgccatg agcctctgca agggttatga ggacccgcat
     1741 ttcgtgccgg ccaaacccag cctgatgaag gccatccagg acgccggcgt gttcgtgtcc
     1801 gtcgggctcg agctggacgg gggctggctg ccgctggtca tcccgggcag ccgcaacccc
     1861 aagatccagc ccgggcaatc cggcttcgtg gacgcctccg agggcatcga cgtgctggac
     1921 aagccggtcg gcacggtctc gcgcgccgag gg
//
LOCUS       DASTJJ010000266         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_528837, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000266 DASTJJ010000000
VERSION     DASTJJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528837"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFR02_03255"
     CDS             <1..468
                     /locus_tag="VFR02_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET9869502.1"
                     /translation="LHGGANEQVMLMLEKVGESSKAESFIRDALARKDRIMGFGHRVY
                     KNGDPRARHLMRLSEAMGKLKGDLKWYEMSRVIDQVVQKEKGLLPNVDFYSASTYHYL
                     GIPTDIFTPIFACSRITGWTAHVIEQLAHNRLIRPRAEYIGARDQHYVPIDKR"
     gene            complement(537..896)
                     /locus_tag="VFR02_03260"
     CDS             complement(537..896)
                     /locus_tag="VFR02_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9869503.1"
                     /translation="MKRPSSGIYANAGASLRRLRKEKGFTLDELSERAEISLSYLSHI
                     ERGTRQAPLTTLETLARILGINLYDLFQPLKPARPAARESRSAFDAKIAGLLPALSEG
                     QKKNLHGLMKEMRRKNR"
BASE COUNT          212 a          309 c          354 g          195 t
ORIGIN      
        1 ctgcacggcg gcgccaacga gcaggtgatg ctcatgctgg aaaaagtggg ggagagctcc
       61 aaggcggagt ccttcatccg cgacgccctg gcccgcaagg accgcatcat ggggttcggg
      121 caccgcgtct acaagaacgg cgacccgcgg gcgcgccatc tgatgcgcct gtccgaggcc
      181 atgggcaagc tcaagggcga cctgaagtgg tacgagatgt cccgggtcat cgaccaagtg
      241 gtccagaagg aaaagggcct gttgcccaac gtggatttct actcggcctc cacctaccac
      301 tatctgggca tccccacgga catcttcacg cccatcttcg cctgcagccg catcacgggc
      361 tggacggccc acgtcatcga gcagctggcc cacaaccggt tgatccggcc ccgggccgag
      421 tatatcggcg cccgggacca gcattacgtg cccatcgaca agcgctgacg aaggacgaaa
      481 acaaaaggcc gggcccccgg gggcccggcc ttttgttttt ggggaggacg ggggccttac
      541 cggttcttgc ggcgcatttc cttcatcaag ccgtggaggt tctttttctg cccctcggac
      601 aaggccggca acaggccggc gatcttggcg tcgaaggcgg accgggattc gcgggcggcc
      661 ggcctggcgg gcttcagggg ctggaaaagg tcgtagaggt tgatgcccag gatgcgggcc
      721 agggtctcca atgtggtcag gggcgcctgg cgggtgccgc gttcgatgtg gctcaggtag
      781 ctgagggaga tctcggcccg ttcggacagt tcgtccaggg tgaacccctt ttccttgcgt
      841 aggcggcgca gggacgcccc cgcgttggcg tagatgccgc tggaaggtcg tttcatggcc
      901 gtcactatgc caggattggg agggcgaaaa aagcgacaaa gggtcaaata acattttgac
      961 ttaaagaaaa atatgactta ggatttatgc actttccaag tcgggaggcc tggccgtgga
     1021 aatggactgg gaaaaggtca tccacaagca tttcaccaag cggggcttcc
//
LOCUS       DASTJJ010000267         1348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_39611, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000267 DASTJJ010000000
VERSION     DASTJJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39611"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFR02_03265"
     CDS             <1..664
                     /locus_tag="VFR02_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET9869504.1"
                     /translation="VFLGVLLSLGSGSAVANSVAGIILTYMRPFQVGDRVMIGDTMGD
                     VAEKNLLVTRIQTIKNEVVTIPNSMVLGNHMINYSSPATRQGLILHTAVTIGYDVPWR
                     KVHELLTAAALSTPDLLAEPKPFVLQTSLGDYSVAYELNAYTDRPHRMAGIYSDLHQA
                     IQDQFNQAGIEILSPQYAALRDGNRSTVPEAHRPGGYRAPGFRVENAGPGPGKNPPEP
                     GK"
     gene            743..818
                     /locus_tag="VFR02_03270"
     tRNA            743..818
                     /locus_tag="VFR02_03270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Ala,seq:ggc)
     gene            978..>1348
                     /locus_tag="VFR02_03275"
     CDS             978..>1348
                     /locus_tag="VFR02_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9869505.1"
                     /translation="MYLREDLEKLEEQVLAPYAQKAAQSKGRWHPEAEHVYRTAFQRD
                     RDRVIHSSAFRRLQYKTQVFVNHEGDYYRTRITHTLEVAQIARSIARTLGLNQDLTEA
                     VALAHDLGHTPFGHMGERVLN"
BASE COUNT          249 a          467 c          394 g          238 t
ORIGIN      
        1 ggtcttcctg ggcgtgctct tgtccctggg ttcgggctcc gcggtggcca acagcgtggc
       61 ggggatcatc ctcacctaca tgcgtccctt ccaagtgggg gaccgggtga tgatcgggga
      121 cacgatgggc gacgtggcgg agaagaacct gctggtcacc cgcatccaga ccatcaagaa
      181 cgaggtggtg accatcccca attcgatggt gctggggaac cacatgatca actacagctc
      241 gccggccacc cggcaggggc tcatcctgca cacggcggtc accatcggct acgacgtgcc
      301 ctggcgcaaa gtgcatgaac tgttgacggc ggccgccctt tccaccccgg acctgctggc
      361 cgaacccaag cccttcgtcc tgcagaccag cctgggggac tacagcgtgg cctacgagct
      421 gaacgcctac acggaccgcc cccaccgcat ggcgggcatc tattcggacc tccaccaggc
      481 catccaggac cagttcaacc aggccgggat cgagatcctg tcgccccaat acgcggccct
      541 ccgggacggc aaccgcagca cggtgccgga agcccaccgg cccggcggtt atcgggcgcc
      601 cggcttccgg gtcgagaacg ccggccccgg ccccgggaaa aacccccccg aaccggggaa
      661 gtgaccgggc cccctttggg cgattgtttg acttgttttt ttgccggttt tcgttagaat
      721 taccgtccgc tgtcagaccg gagggggcgt agctcagctg ggagagcact tccatggcat
      781 ggaaggggtc gtgggttcga tccccatcgc ctccaccatt ttcccgatcg accgtcgtcc
      841 cgcgacggat ccagggaaaa tgaaggtccc gattcctgat aataacggct cgattagact
      901 gcggcaccat tcggtttttc ccgccttatc tttccgaacc tctttatctt ttttttgaca
      961 accgttacca tcggcccatg tacctgcgtg aagacctgga aaaactcgag gaacaagtcc
     1021 tggcgcctta cgcccagaag gccgcccagt ccaagggccg ctggcacccg gaggccgagc
     1081 atgtgtaccg cacggccttc cagcgcgacc gggaccgggt catccattcc tccgccttcc
     1141 gccgcctcca gtacaagacc caggtcttcg tcaaccacga gggggactat taccgcaccc
     1201 gcatcaccca caccctggaa gtggcccaga tcgcccggtc catcgcccgc acgctgggct
     1261 tgaaccagga cctgaccgag gcggtggccc tggcccacga cctgggccac acgcccttcg
     1321 gccacatggg cgagcgggtg ctgaacga
//
LOCUS       DASTJJ010000268         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_650956, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000268 DASTJJ010000000
VERSION     DASTJJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650956"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFR02_03280"
     CDS             <1..480
                     /locus_tag="VFR02_03280"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET9869506.1"
                     /translation="LMIEAVELKLRHWKREDKFHGKFSGQGHYFGYEGRCADPSNFDA
                     DYCYSLGATAAYLLASGLTGYLSSVRNLLKPADQWKAGGVPLTMMMNLERRHGKNKPV
                     IQKALVDLKGRPFKAFAAKRAQWALEDDYTSPGPIQFWGPAAVADAPPETLRLERGK"
     gene            752..1258
                     /gene="rpsF"
                     /locus_tag="VFR02_03285"
     CDS             752..1258
                     /gene="rpsF"
                     /locus_tag="VFR02_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9869507.1"
                     /translation="MSNFLKYDVIYILDPNATTEDAAAVSAKVEQITADAKGTVLKKD
                     DWGKRRLAYLVQKHREGHYIFYHLSLPPDAVAEVQRNLRLMEKVIKFSIVQDTISHLK
                     AKVKPVRVKTSTGTEGSSSRPGYSRPTNPSTIRSASSHVTSSPAPAAAPAPAPAPAPS
                     AEAQNSPS"
     gene            1286..>1469
                     /gene="ssb"
                     /locus_tag="VFR02_03290"
     CDS             1286..>1469
                     /gene="ssb"
                     /locus_tag="VFR02_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9869508.1"
                     /translation="MPNFNKVFLMGNLTKDPELRYTAGGAPVANLRMAINRTYQSQSG
                     EKKEDVCFVTVVVWRKQ"
BASE COUNT          300 a          492 c          435 g          242 t
ORIGIN      
        1 ctgatgatcg aggcggtgga gctgaagctg cgccattgga agcgggagga caaattccac
       61 ggcaagttct cgggccaggg ccattatttc ggctacgagg gccgttgcgc ggacccttcc
      121 aacttcgacg ccgactactg ctattcgctg ggcgccacgg ccgcctatct gctggcctcg
      181 ggtctgaccg ggtacctgtc cagcgtgcgc aacctgctga agcccgccga ccaatggaag
      241 gccgggggcg tgcccctgac catgatgatg aacctggagc gccgccacgg caagaacaag
      301 ccggtcatcc agaaggcgct ggtggacctg aagggccgcc ccttcaaggc cttcgcggcc
      361 aagcgcgccc agtgggcgct ggaggacgac tacaccagcc ccggccccat ccaattctgg
      421 ggcccggccg cggtggccga cgcgccgccc gagacccttc gcctcgagcg ggggaagtaa
      481 agataaaggc tgattttcac caccaaggca ccaagccacc aagagctgaa aaaccgataa
      541 aatttgattt ttggggtcaa cttggtgcct tggtgtcttg gtggtgatgc gggtagttgt
      601 tttcatattg aatttgaagg ctttcccgac ctatactccc cccctgcttt gaagaagccc
      661 tcaaaagccc ctccaaagcc ctgtccctcc aagcaagacc ggggggacca tcccgctcca
      721 aggaacggga atccacaggg aggtcaattt catgtcgaat ttcttgaagt acgatgtcat
      781 ctacattttg gacccgaacg ccaccacgga agacgcggcg gccgtttcgg ccaaggtgga
      841 gcagatcacg gccgacgcca agggcacggt cctgaaaaag gacgactggg gcaagcgccg
      901 cctcgcctac ctggtgcaga agcaccggga aggccactat atcttctacc acctgtccct
      961 gccgcccgac gcggtggccg aggtgcagcg caacctgcgc ctgatggaga aggtcatcaa
     1021 gttctccatc gtccaggaca ccatcagcca cctcaaggcc aaggtgaagc cggtgcgggt
     1081 caagaccagc acgggcaccg aaggctcctc ctcgcgcccg ggctacagcc gcccgaccaa
     1141 cccctcgacg atccgctcgg cctcgtccca cgtgacgtcg tcgccggctc cggccgccgc
     1201 gcccgccccg gcccccgcgc cggcgccttc ggcggaagcc cagaactcac cttcctaacc
     1261 gccttcaacc gaaaggggag cgcgcatgcc caacttcaac aaggtcttcc tgatggggaa
     1321 cctcaccaag gatcccgagc tccgctacac cgcgggcggg gccccggtgg ccaatcttcg
     1381 gatggccatc aaccggacct accagtccca gtcgggcgag aagaaggaag acgtctgttt
     1441 cgtgaccgtc gtggtctggc gcaagcagg
//
LOCUS       DASTJJ010000269         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1751646, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000269 DASTJJ010000000
VERSION     DASTJJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751646"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFR02_03295"
     CDS             <1..660
                     /locus_tag="VFR02_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869509.1"
                     /translation="DKNRIVFEGYGTGPNQLYTINADGTGLAVVPNTIGAGAPAWSPD
                     GTDLVFVSGALSPAQLCAIHTDGTGLVQLTHETNGVSLEDFACSPDGSRIAYTTPGGT
                     KIMDANGGNVVDFPVTMQQMDWSPDGTSFVFCAPTGVSGGNTIDQVFRVNADGTGLVM
                     LTDTDSHGGACWSRDGSRIVVVDLWANGPPNMSLINPDGSGPVPIPGAYIYYCYQPCW
                     H"
     gene            complement(888..>1062)
                     /locus_tag="VFR02_03300"
     CDS             complement(888..>1062)
                     /locus_tag="VFR02_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9869510.1"
                     /translation="KIAVSAGSKEEADKIFQGLSAGGSVEMPITDSPWGTYFSMFRDK
                     YGIEWTVEFDPKK"
BASE COUNT          201 a          327 c          324 g          210 t
ORIGIN      
        1 gataaaaacc ggatcgtgtt cgagggatac ggcacgggcc ccaaccagct ctatacgatc
       61 aacgcggacg ggaccggatt ggcggtcgtc ccgaacacca tcggggcggg ggcgccggcc
      121 tggtccccgg acggcaccga cctcgtcttc gtttccgggg ccctgtcgcc ggcccagctt
      181 tgcgccatcc atacggacgg gaccggcctg gtccaattga cgcatgaaac caacggggtg
      241 tccctggagg acttcgcctg ttcgcccgac ggaagccgga tcgcctatac cacgcccggc
      301 gggacgaaga tcatggatgc caacgggggg aacgtggtgg atttcccggt gaccatgcag
      361 cagatggact ggtcgccgga cgggacgagt tttgttttct gtgcccccac gggcgtgtcg
      421 ggcggcaata ccatcgacca agtcttccgg gtgaacgccg atgggacggg cctggtgatg
      481 ttgaccgata cggacagcca cggcggggcc tgctggtcca gggatgggag ccgcatcgtg
      541 gtggtggacc tatgggcgaa cggaccgccc aatatgtccc tgatcaaccc ggacggttcc
      601 ggccccgtcc ccatccccgg ggcctacatc tattactgct accagccctg ctggcactga
      661 ggcggaagcg gccgacggaa tcaacgatca attttccaac gttttgcagg atcgcgtgct
      721 tggcaaatcc cacaaactaa tttccctctc cccttggagg gagagggtca gggtgagggg
      781 gaaacaataa tttttcaccc ggatatcagc cttccccccg gcttttggtc cttgggccaa
      841 cgggagcgtc gaaaaccatg tgtgggtttg ccaagggtgt tttccagcta tttcttagga
      901 tcgaactcca cggtccactc gatcccgtat ttgtcccgga acatggaaaa ataggtaccc
      961 cagggactat cagtgatggg catttccacg ctcccgcccg ccgaaagccc ctggaagatc
     1021 ttgtccgcct cttccttgct ccccgcgctg acggcgatct tg
//
LOCUS       DASTJJ010000270         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_284923, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000270 DASTJJ010000000
VERSION     DASTJJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284923"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..877)
                     /locus_tag="VFR02_03305"
     CDS             complement(20..877)
                     /locus_tag="VFR02_03305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9869511.1"
                     /translation="MSASTLVLAAGSLGDSLLTLPALQFLQTKGPVTLAGTSPYQKLG
                     AACLGVEKVIALDPLLDALHRKDPNTLPGTPGGLYVFFKDKDAGLAESLAAYPGWAVH
                     WPPQSFTDFLKQERWVGHYWLDLVGHPDPSPIPRLVLTEEMRTRGAGLCARLGTPRPF
                     VIHPGSGSPAKNAPLSFFRKAAEKTVQETTRAVLVLWGEAETGWLDDIRKAFEGIPRA
                     TLLPGPLSLIDLAALLSQAGGYLGNDSGVTQLASACGAKTFAVFNTTDPRLWGPQEAV
                     ILAAMGGNF"
     gene            892..>1131
                     /locus_tag="VFR02_03310"
     CDS             892..>1131
                     /locus_tag="VFR02_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869512.1"
                     /translation="MNEEEREGAWILSGVADQAKPDGKNENGLDVCHGFFIIGSEAAG
                     TRPIDRLFPKKSENWTKSPASPQAPGGLSLISLHFN"
BASE COUNT          217 a          324 c          384 g          206 t
ORIGIN      
        1 tgcggtgaag gggttggttt taaaagttcc cgcccatggc ggccaagatc accgcttcct
       61 gcgggcccca gagcctgggg tcggtggtgt tgaagaccgc gaaggttttg gcgccgcagg
      121 ccgaggccag ttgggtcacc ccgctgtcgt tgcccaggta tccgcccgct tgggacagca
      181 gcgccgccag gtcgatcagg ctgaggggcc cgggcaaaag ggtggcccgg gggatcccct
      241 cgaaggcctt gcggatgtcg tccagccagc cggtttccgc ttccccccac aagaccaaca
      301 ccgcacgggt ggtttcttgg accgtcttct cggccgcctt gcggaagaag gaaagcgggg
      361 cgttcttggc ggggctgccg ctgcccgggt ggatcacaaa gggccggggc gttcccagcc
      421 tggcgcaaag ccccgcgccc cgcgtccgca tttcttccgt caagaccagc cgggggatgg
      481 gcgaggggtc cggatggccc accaggtcca gccaataatg gccgacccag cgttcctgct
      541 tgaggaagtc cgtgaaggat tggggaggcc aatggaccgc ccatcccgga taagccgcca
      601 ggctttccgc gagtccggca tccttgtcct tgaaaaaaac ataaagcccg cccggcgtcc
      661 cgggcagggt gttggggtct ttgcggtgga gggcgtccaa caagggatca agggcgatga
      721 ccttttccac ccccaggcag gcggctccca gcttttggta aggggaagtg ccggccaggg
      781 tgaccgggcc tttggtttgc aggaattgca gggcaggcag ggtgagcagg ctgtcgccca
      841 ggctgccggc ggcaaggacc aaagtggaag cgctcatagg gtggggcctg ggtgaacgaa
      901 gaggaacggg aaggggcatg gatcttgtcc ggcgtagccg accaggcgaa gccggatggc
      961 aagaatgaaa atgggttgga tgtctgtcat ggatttttta ttataggcag tgaagccgca
     1021 ggcacccgcc caattgacag gctttttccc aaaaagtcag aaaattggac aaaatcaccc
     1081 gcttccccac aagcccccgg gggattatcg ttaatttcgc ttcattttaa c
//
LOCUS       DASTJJ010000271         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_407477, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000271 DASTJJ010000000
VERSION     DASTJJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407477"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1183
                     /locus_tag="VFR02_03315"
     CDS             143..1183
                     /locus_tag="VFR02_03315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9869513.1"
                     /translation="MTRPPRHILVSRTDKIGDLLLSLPVFQTLRAAFPKAKITALVSP
                     YAKEIVQGHPAVDSVELLHPGEGFLSLQSRFRKLAPDVFLALYPRPAVALAAWAARVP
                     LRVGTAFRWYGFTFNEKIRAHRSACERHEADYNLDLARVLGVERTADRILVPVPAADQ
                     AFADDLLKEKGVPKAARFVAVHPGHKGSALNWSPRRYAEVVTRLLVGGRRVVLTAGPD
                     ETALMARVADFLGPEAKRRKPVMLIGECGLKQLAGVYRRADVFLSGSTGTMHLAAAVG
                     TPTVALFCPIPATTPVRWGPWGNRATVLMPEGLTCPDCQRGFCRRHDPMDGIPVERVL
                     EALKPYLGRPRA"
     gene            1176..>1574
                     /locus_tag="VFR02_03320"
     CDS             1176..>1574
                     /locus_tag="VFR02_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9869514.1"
                     /translation="MRRGPLPAAVLAVLGLTLPGCSLLFVAKGETRYIPSSWKTPPAS
                     TSLAGEMEKNPPPQEAPASPAGKARHQVAPGDSLWKLAKHYYGKGAAYPKIASANGLD
                     AEKPIRVGTWLTIFMPGQAEPLPPPGTSTPG"
BASE COUNT          237 a          574 c          516 g          247 t
ORIGIN      
        1 acacccccga ttccatcccc gagaaggaca cggtggcctc ctacggcgga cgggtggccg
       61 tggtgggcga ccccaaggac cacgcctcca agaacctgat ccaggagatc gccgaaaagt
      121 acgggcccct ttccccgagc cgatgacccg acctccccga cacatcctgg tttcccggac
      181 cgacaagatc ggcgacctgc tgctttccct gccggtgttc cagaccctgc gggccgcctt
      241 ccccaaggcc aagatcaccg ccttggtcag cccctacgcc aaggagatcg tccaaggcca
      301 cccggcggtg gactcggtgg agctcctcca tccgggcgag ggcttcctgt ccctgcaaag
      361 ccgtttccgg aagctcgccc ccgatgtgtt cctggccctg tacccgcgcc cggccgtcgc
      421 cctggcggct tgggcggccc gcgtccccct gcgggtgggt acggctttcc ggtggtacgg
      481 tttcaccttc aatgaaaaga tcagggcgca ccgctcggcc tgcgagcggc atgaggccga
      541 ctacaacctg gacctggccc gggtcctggg cgtggaacgg acggcggaca ggatcctcgt
      601 ccccgtcccc gccgccgacc aggccttcgc cgacgacctg ctcaaggaaa agggcgtccc
      661 caaggccgcc cggttcgtgg ccgtgcatcc gggccacaag ggttcggcct tgaactggag
      721 cccccggcgt tacgccgagg tggtcacccg gctcttggtg gggggcaggc gggtggtctt
      781 gaccgcgggg cccgacgaga ccgcgctcat ggcccgggtg gcggatttcc tggggccgga
      841 ggccaagcgc cgcaaacccg tgatgttgat cggcgagtgc ggcttgaagc agctggcggg
      901 cgtctaccgg cgggctgacg tgttcctgtc gggctccacg ggcaccatgc acctggcggc
      961 ggcggtgggc acgcccaccg tggccctttt ctgccccatc ccggccacca cgccggtgcg
     1021 ttggggcccc tggggaaacc gcgccaccgt gttgatgccc gagggcctga cctgccccga
     1081 ttgccaaagg ggtttttgcc gccggcacga ccccatggac ggcatcccgg tggagagggt
     1141 cctggaggcc ctgaaaccct acctgggccg tccccgtgcg taggggcccg ctccccgcgg
     1201 cggttttggc cgtcctgggc ctgaccctgc ccggttgctc gctgctgttc gtggccaagg
     1261 gcgagacccg ttacatcccc tccagttgga agacaccgcc cgcttccacc tccctggcgg
     1321 gggaaatgga aaagaacccg ccgccccagg aagctcctgc gtccccggcg gggaaggccc
     1381 gccaccaggt ggccccgggg gacagcctct ggaaactggc caaacattat tatggaaagg
     1441 gcgcggctta tcccaagatc gcctcggcca acggcttgga cgccgaaaag cccatccggg
     1501 tgggcacctg gctgaccatc ttcatgccgg gccaggccga accgcttccc cccccgggaa
     1561 cctccacgcc gggg
//
LOCUS       DASTJJ010000272         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1630445, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000272 DASTJJ010000000
VERSION     DASTJJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630445"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /gene="murA"
                     /locus_tag="VFR02_03325"
     CDS             <1..917
                     /gene="murA"
                     /locus_tag="VFR02_03325"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9869515.1"
                     /translation="GCAIGARPIDMHLRGVEELGAKVTLAHGYVEAEGKLKGAVIHLA
                     GPHGPSVGATVNVLLAATAAEGTTVIESAATEPEVEDLVNFLKAMGADIQGGGTSRLV
                     IRGGKPLTGATYGIIPDRVEAGTFMTAAAITGGEVTLAQVAPPHLSDVTRLFRSVGVT
                     VEEGPDWMKVTGNGAYRPADVRVAPYPGFPTDMQAQVMALLAVVPGLSVITETIWENR
                     FMHVFELNRMGADIKIEGNNALVKGVKRLTGAPVMASDLRASAALILAGLVADGETLV
                     QRIYHLDRGYEQIEKKLSALGADVERIN"
     gene            1042..2397
                     /gene="hisD"
                     /locus_tag="VFR02_03330"
     CDS             1042..2397
                     /gene="hisD"
                     /locus_tag="VFR02_03330"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET9869516.1"
                     /translation="MRILNYSNPKDQKTVAKLMRREALVAIRGAGSRSALTRKVFGKA
                     LSPDQAVDQILKAVRDKGDAALFSYTRKLDGFAASKANVQVTPEEIRRALKGVPKLLL
                     DSLKTAITQIRAFHEKERPRDWVEKSRDVNWWQHWTALDQVGVYVPGGLAVYPSSVLM
                     NVIPARVAGVKRVVMATPVKKSGQVDPVILAAAHLAGVDKIFKIGGAQAVAALAYGTQ
                     TVPAVDKITGPGNLFVALAKRKVFGQVGIDGFAGPSEVLILSDGTVSPAWVAADILAQ
                     AEHDEMACCLLVTPSEKLAREVSKEVELQLELLSRRQVASDSLKSRGAVLLVKNRAQA
                     LEAANRFAAEHLEILAKDPNPWFQGIRHAGAVFVGPLSPVAFGDFTAGTNHILPTSGT
                     ARFASGLSVFDFMKRTNVLEVKEEGVRKLGRPTVFLAEQEGLTGHAQSVLFRMGSGAK
                     P"
     gene            2394..>2749
                     /locus_tag="VFR02_03335"
     CDS             2394..>2749
                     /locus_tag="VFR02_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9869517.1"
                     /translation="MKPLKDRFRPEVLRMTGYVPGEQPQDPRTLKLNTNENPYAPPAV
                     VLKAVRAAADARLRLYPEPTASTLRRRLAALYRWPLEGILVGNGSDEILSILFRACVG
                     KGDLVQYPDLTYSLYPV"
BASE COUNT          483 a          915 c          923 g          428 t
ORIGIN      
        1 ggggctgcgc catcggcgcc cggcccatcg acatgcacct gcggggcgtg gaggagttgg
       61 gcgccaaggt caccctggcc cacggttacg tggaggccga gggcaagctg aagggcgccg
      121 tcatccacct ggccggaccc cacgggccca gcgtgggcgc cacggtgaac gtgctcctgg
      181 ccgccacggc cgccgagggc accacggtca tcgaatcggc ggcgaccgaa cccgaggtgg
      241 aggacctggt gaacttcctg aaggccatgg gggccgacat ccagggcggc ggcacctcgc
      301 gcctcgtcat ccggggcggc aaacccctca cgggcgccac ctacggcatc atccccgacc
      361 gggtcgaggc gggcaccttc atgaccgccg ccgccatcac gggcggggaa gtgaccctgg
      421 cccaggtggc ccccccgcac ctgtcggacg tgacccgctt gttccgctcg gtgggggtga
      481 cggtcgagga agggccggac tggatgaagg tgacggggaa cggggcctat cgaccggcgg
      541 acgtgcgcgt ggcgccctac ccggggttcc ccaccgacat gcaggcccag gtcatggcgc
      601 ttttggccgt ggtgcccggg ctttcggtca tcacggagac catctgggaa aaccgcttca
      661 tgcacgtttt cgagttgaac cgcatgggcg cggacatcaa aatcgagggg aacaacgccc
      721 tggtcaaggg cgtgaagcgc ctgaccggcg cccccgtaat ggcgtcggac cttcgggcca
      781 gcgcggcgct catcctggcc ggcctggtgg ccgacggcga gaccctggtg cagcgcatct
      841 accacctgga ccgcggctac gagcagatcg aaaagaagct gtccgccctg ggcgcggacg
      901 tcgaaagaat aaattaaaaa acctgtttca ccaccaaggc accaagactc caaggaaggc
      961 aacaaaagat cggatccgaa gattgatttc ttggtgtcct tggtgccttg gtggtgaaat
     1021 gttgaaaaga atgaggtttt tgtgagaatt ctcaattatt cgaacccaaa agaccaaaag
     1081 accgtcgcta agctcatgcg ccgggaggcg ctcgtcgcca tccggggggc ggggtcgcgc
     1141 tcggccctga cccggaaggt cttcggcaag gccctcagcc ccgaccaggc ggtggaccag
     1201 atcctcaagg ccgtgcgcga caagggcgac gcggcgcttt tttcctacac ccgcaagctg
     1261 gacggcttcg ccgcctcgaa ggccaacgtc caggtgaccc ccgaggagat ccggcgggcc
     1321 ctgaagggcg tccccaagct cctgctggat tcattgaaga ccgccatcac ccagatccgg
     1381 gcctttcacg agaaggaaag gccccgggat tgggtggaaa aatccaggga cgtgaattgg
     1441 tggcagcatt ggaccgccct ggaccaggtg ggcgtctatg tgccgggggg cctggcggtc
     1501 tacccttcct ccgtgctgat gaacgtcatc ccggcccggg tggcgggggt gaaaagggtg
     1561 gtcatggcca cgccggtgaa aaagtcgggc caggtggatc cggtcatcct ggcggcggcc
     1621 catttggcgg gcgtggacaa gatcttcaag atcggcggcg cccaggccgt ggcggccctg
     1681 gcctacggca cccagaccgt cccggcggtg gacaagatca ccggcccggg caacctcttc
     1741 gtggccctgg ccaaacgcaa ggtgttcggg caggtgggca tcgacggctt cgccgggccc
     1801 agcgaggtgc tcatcctcag cgacggcacc gtgtcgcccg cctgggtggc ggcggacatc
     1861 ctggcccagg ccgagcatga cgagatggcc tgctgcctcc tggtgacccc ctcggaaaaa
     1921 ctggcccggg aagtctcgaa ggaagtggag ctccagctcg agcttttgtc gcggcgccag
     1981 gtggcgtccg attccctcaa gagccggggg gccgtcctgt tggtcaagaa ccgcgcccag
     2041 gccttggagg ccgcgaaccg cttcgccgcc gagcacctgg agatcctggc caaagacccc
     2101 aacccctggt tccagggcat ccggcacgcc ggcgcggtct tcgtggggcc cttgtccccc
     2161 gtggccttcg gcgacttcac ggccggcacc aaccacatcc tgcccaccag cggcacggcc
     2221 cgcttcgcct cggggctttc ggtgttcgac ttcatgaagc gcaccaacgt gctggaagtg
     2281 aaggaggagg gcgtgcgcaa gctgggccgg cccacggtct tcctggccga gcaggaaggc
     2341 ctgaccggcc acgcccagag cgtgctcttc cggatgggtt ccggggccaa accgtgaaac
     2401 cattgaagga tcggttccgc ccggaggtcc tccggatgac gggctatgtg cccggcgagc
     2461 agccccaaga cccccgcacc ctcaaactca acaccaacga gaacccctat gcgccgccgg
     2521 ccgtggtgtt gaaggccgtg cgcgccgccg cggacgcgcg cttgcgtctt taccccgaac
     2581 ccaccgcctc gaccctgcgc cgacgcctgg cggccctgta ccgctggccg ctggagggca
     2641 tcctggtggg caacgggtcc gacgagatat tgtccatcct gttccgggcc tgcgtgggca
     2701 agggcgacct ggtgcagtat cccgacctca cctacagcct ctatcccgt
//
LOCUS       DASTJJ010000273         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_651480, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000273 DASTJJ010000000
VERSION     DASTJJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651480"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1365)
                     /locus_tag="VFR02_03340"
     CDS             complement(118..1365)
                     /locus_tag="VFR02_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9869518.1"
                     /translation="MLFLALKQLLSRPRQTLLALLGITFGTAAFLILSGMMLGFREYF
                     IQRLINTAAHISITAEDPQLGEHSLDPAFFPGALVRWIVPPVNHDETPHLEHPQGWFD
                     RLEADPRVEAYAPQLGAQALVTRGKLSRTINLVGVEARRQARATDVASDMVEGNFMDL
                     DKGGFQVVIGQSLAQKLGLRLGDTLDLVNTRGEVRPAKVAGLFNTGIDQIDQTTAYAP
                     LHDVQQVAQTPGEVSQIIVKLKDITQARRVANEWAQGSREKVQSWDEANANFFTVFRM
                     QDMIRYILSGVVLMIAAFGIYNILNMIVLQKRGEIAILRSMGFEASDIVRLFLSQGLV
                     LGFLGGLLGLGVGTLVCLYVQSIRLSTGMGMSSVDHMLIAWHAYFYWMAFLLAFLSGL
                     LAGYLPARLASRMDPIDILRSEG"
     gene            complement(1365..2159)
                     /locus_tag="VFR02_03345"
     CDS             complement(1365..2159)
                     /locus_tag="VFR02_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9869519.1"
                     /translation="MSPRLPSGWKARAAWLAGGLAPLLLAAVWLSHGRAARGLHPRVG
                     PVVQAVYGIGTATARHTFDLKLGVADTLEKLYAREGDAVPKGARLVALADLPPVRAPF
                     AGVVTSLPFKEGETLFPQVPVLTLTDLKDPYIVVSLEQEGALRVRRGQPAVVSFETLR
                     DDRLAGTVSAIYPQDGQFLVNIEVPHMPEGALVGMTTDVAIRVEDRASALQVPLTLVS
                     QGQVRVERGGAERTVAVKLGAGDGTWAEVVEGDLQPSDLLLPPRNP"
     gene            complement(2156..>2714)
                     /locus_tag="VFR02_03350"
     CDS             complement(2156..>2714)
                     /locus_tag="VFR02_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9869520.1"
                     /translation="AAYAGSINPKERTLSPSTASPSLKNLSRIHRELGGLFHRHQEAL
                     LSGDLARARALFQDYEAALRSHTREEDEVLIPLYRERALPPPRGGSPEYFTGEHQKIN
                     EWLGRLRLRLSRLTGGAKGSRALLALLDDEAFFKRYLEHHSLREDRVFYPVLDRLLTP
                     RERTGLARLLSFTLEDQDLSEADPS"
BASE COUNT          445 a          890 c          967 g          412 t
ORIGIN      
        1 gaccggcccg tgatggccac gaactcgccg tccccgatct ccaggtccag gcccttgagc
       61 acgggggtgg ggggctgccc gaaggatttg acgatgccga ccgcttggag ccccatgtca
      121 gccctccgac ctgaggatgt cgatggggtc catgcggctg gccagccggg cgggcaggta
      181 gccggccagc agtccggaca ggaaggccag caggaaggcc atccaataga aataggcgtg
      241 ccaggcgatg agcatgtggt cgaccgagct catgcccatg ccggtggaaa gccggatgga
      301 ctgcacgtaa aggcagacca gggtgcccac ccccaggccc agcagcccgc ccaggaagcc
      361 cagcaccagg ccctgggaca ggaacagccg gacgatgtcc gaggcctcga accccatgga
      421 ccgcaggatg gcgatctccc cgcgcttttg cagcacgatc atgttgagga tgttgtagat
      481 gccgaaggcc gcgatcatca gcaccacgcc gctgaggatg tagcggatca tgtcctgcat
      541 gcggaagacg gtgaagaagt tcgcgttggc ttcgtcccag ctctggacct tctcgcggct
      601 cccctgggcc cactcattgg ccacccggcg ggcttgggtg atgtccttga gcttcacgat
      661 gatctgggag acctcgccgg gcgtttgggc gacctgctgg acgtcgtgca ggggcgcgta
      721 ggcggtggtc tggtcgatct ggtcgatccc ggtgttgaag aggcccgcca ccttggcggg
      781 ccgcacctcg ccccgggtgt tcaccaggtc gagggtgtcg cccaggcgaa ggccgagctt
      841 ttgggccagg gactggccga tcaccacctg gaagccgccc ttgtccaggt ccatgaagtt
      901 gccttccacc atgtccgatg ccacgtcggt cgcgcgggct tggcggcgcg cttccacgcc
      961 caccaggttg atggtgcggg acagcttgcc ccgggtgacc agggcctggg cgcccaactg
     1021 gggcgcgtag gcctccaccc ggggatcggc ctccagccgg tcgaaccaac cctggggatg
     1081 ttccaggtgg ggcgtctcgt catggttgac cggcggcacg atccagcgca ccaaggcgcc
     1141 cgggaagaaa gcgggatcca ggctgtgttc gcccaattgg gggtcctcgg cggtgatgct
     1201 gatgtgggcg gccgtgttga tcaaccgttg gatgaagtat tcccggaacc ccagcatcat
     1261 gcccgacagg atcaggaagg ccgccgtgcc gaaggtgatg cccaaaagcg ccaacagggt
     1321 ctggcggggc cgggacaaga gttgtttgag ggccaggaaa agcatcaggg gttccggggc
     1381 ggcagcagca ggtccgaggg ttggaggtcg ccttccacca cctcggccca ggtgccgtcc
     1441 ccggccccca gtttcacggc gaccgtccgt tcagcccccc cgcgctcgac ccggacctgt
     1501 ccctgggaca ccagcgtcag gggcacctgc agcgccgagg cgcggtcctc cacccggatg
     1561 gccacgtcgg tggtcatgcc caccagcgcc ccctcgggca tatggggcac ctcgatgttc
     1621 accaggaact gcccgtcctg ggggtagatg gcggagaccg tcccggccag gcggtcgtcc
     1681 cgcagggtct cgaagctcac caccgcgggt tgcccccggc gcacccgcaa ggcgccttcc
     1741 tgttccagcg acaccacgat gtaggggtcc ttcaggtccg tgagggtcag gacggggacc
     1801 tgggggaaca gggtttcccc ctccttgaag gggagcgagg tcaccacgcc cgcgaaaggc
     1861 gcgcgcaccg gcggcaggtc ggccagcgcc acgaggcgcg cgcccttggg cacggcgtcg
     1921 ccttcccgcg cgtaaagttt ttccaaggtg tcggccacgc ccaacttcag gtcgaaggtg
     1981 tgccgggccg tggcggtgcc gatgccatag acggcctgga ccaccggccc cacccggggg
     2041 tgaaggccgc gcgccgcccg gccatgggac agccaaaccg ccgccaagag caggggcgcc
     2101 agcccccccg ccaaccaagc ggcccgggcc ttccagccgg aagggaggcg cgggctcatg
     2161 acgggtccgc ctcggagagg tcctggtcct ccagggtgaa ggacagcagg cgggccaggc
     2221 ccgtgcgttc ccggggggtg agcagccggt ccaggacggg ataaaagacg cggtcttccc
     2281 gcaggctgtg gtgttccagg tagcgcttga agaaggcctc atcgtccagc agggcgagga
     2341 gggcgcggga ccccttggcg ccccccgtca ggcgggagag ccgcagccgc aaccggccca
     2401 gccattcgtt gatcttttgg tgctcgccgg tgaagtattc gggggatccg ccccggggcg
     2461 ggggcagggc cctttcccgg tacaggggga tcaggacctc atcctcctcc cgggtgtggg
     2521 aacgcagggc cgcctcgtag tcctggaaga gggcccgggc ccgggcgagg tcgcccgaca
     2581 gcagggcttc ctgatggcgg tggaagaggc cgcccaactc gcggtggatc cggctcaggt
     2641 tcttcaacga gggcgaagcg gtggaagggc tcaaggttct ctccttgggg ttgatggacc
     2701 cagcataggc cgcg
//
LOCUS       DASTJJ010000274         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_773710, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000274 DASTJJ010000000
VERSION     DASTJJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773710"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFR02_03355"
     CDS             complement(<1..1277)
                     /locus_tag="VFR02_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9869521.1"
                     /translation="MDYNLFSHLVVLAALLLVSMFFSAVETSLLSAPRGLIESNAERP
                     GLLGRAFREWSEHPNRILTTVLIGTNSVNVITTLLAAYTSIHLAEVHQWNPVITGTVT
                     SVFVTFVIIVFGEMIPKITARSLASRVVVWLILPIYLIDRLIRPFTTVLARVITFFIP
                     ALSDVSVTQVTEEDIKHMIEVGRQDGTIREDEQDMIHSVFKFSDTTVNQVMIPRTRMF
                     CVDVKTGLEPLLDTIIQKGYSRVPVYKGDVDNIVGVLHTRDLLSIWRNQGLIVVQDLL
                     RKPYFVPETMRVDRLLREFRRGKIHMAIVVDEYGGTSGLVTLEDLVEQIVGEIRDEHE
                     PEDEKSIVKQEDGSYHIEAGTPLDEINEVLGLHLTPKGEITTLSGYLTELNGRVPKKG
                     RVLDEYEATTTILESSEKKVIRVKLVKKDVPYPP"
     gene            complement(1284..1757)
                     /gene="ybeY"
                     /locus_tag="VFR02_03360"
     CDS             complement(1284..1757)
                     /gene="ybeY"
                     /locus_tag="VFR02_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9869522.1"
                     /translation="MRLLVRAGGVRLPVSAAAARRWAGRVLREAGGASLAGRCELSVV
                     FTGDAEVRRLNRRYRGKDKTTDVLAFPLREGKALKPGLPGAGALGDVVISVPQARRQA
                     RAAGKDAVEEMAMLLTHGILHLLGHDHATKRQEKRMFGLQDKILRKLVRRPSGRR"
     gene            complement(1754..>2322)
                     /locus_tag="VFR02_03365"
     CDS             complement(1754..>2322)
                     /locus_tag="VFR02_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9869523.1"
                     /translation="GKTYLAMAMAVSALREGLVERLVLTRPAVEAGEKLGFLPGDLQD
                     KVDPYLRPLYDALYDMVDYEKLQRLFQQRVVEVAPLAYMRGRTLSNAFIILDEAQNTS
                     PEQMKMFLTRTGTGSKVVVTGDATQVDLPRGLASGLIHAREVLQGVPGIGVVSFGPQD
                     VVRHKLVQSIVRAYDEHEASKLPKSPDA"
BASE COUNT          367 a          788 c          770 g          397 t
ORIGIN      
        1 ggcggataag gcacgtcctt cttgacgagc ttcacccgga tcaccttctt ctcgctggat
       61 tccaggatgg tggtcgtggc ctcgtattcg tccagcaccc ggcccttctt gggcacccgg
      121 ccgttgagct cggtcaggta gccgctgagc gtggtgatct cccccttggg ggtcaggtgc
      181 agccccagga cctcgttgat ctcgtccagg ggcgtgcccg cctcgatgtg gtaggagccg
      241 tcctcctgct tgacgatgga cttttcgtcc tcgggctcgt gctcgtcccg gatctcgccc
      301 acgatctgtt ccaccaggtc ctccagggtc accagccccg aggtgccgcc gtactcgtcc
      361 accacgatgg ccatgtggat cttgccccgg cggaactcgc gcaacaggcg gtccacccgc
      421 atggtctcgg gcacgaaata gggcttgcgc agcaggtcct gcaccacgat gaggccctgg
      481 ttgcgccaga tggaaagcag gtcccgcgtg tgcagcacgc ccacgatgtt gtccacgtcg
      541 cccttataga ccggcacccg ggaataaccc ttctggatga tggtgtccag cagcggctcc
      601 aggcccgtct tcacgtccac gcagaacatg cgggtgcgcg ggatcatgac ctggttcacc
      661 gtggtgtcgc tgaacttgaa caccgagtgg atcatgtcct gctcgtcctc ccggatggtg
      721 ccgtcctggc gccccacctc gatcatgtgc ttgatgtcct cctcggtgac ctgggtcacg
      781 ctgacgtcgg acagcgcggg gatgaagaag gtgatgaccc gggccaggac cgtggtgaag
      841 ggccggatga gccggtcgat gaggtagatg ggcaggatca gccacaccac cacccgggag
      901 gccaggctgc gggcggtgat cttggggatc atctcgccga agacgatgat gacgaaggtg
      961 acgaagaccg aggtgaccgt gccggtgatc acggggttcc actggtggac ctccgccagg
     1021 tggatggagg tgtaggccgc cagcagggtg gtgatcacgt tcaccgagtt ggtgccgatg
     1081 agcacggtcg tcaggatgcg gttggggtgc tcggaccatt cccggaaggc gcggcccagc
     1141 aggcccgggc gctcggcgtt ggattcgatc aggccccggg gggcggacag caggctggtc
     1201 tccaccgcgg aaaagaacat ggacaccagc aacagggcgg ccagcaccac caggtgggaa
     1261 aagaggttgt agtccatgtc cgttcaacgg cggcccgaag gccgccgcac cagctttctc
     1321 aagattttgt cctgcagccc gaacatgcgc ttctcctgcc gcttcgtggc gtggtcatgg
     1381 cccaagaggt gcaggatgcc gtgggtgagc agcatcgcca tctcctcgac ggcgtccttg
     1441 cccgcggccc tcgcctggcg ccgggcctgg ggcacgctga tcaccacgtc gcccagggcc
     1501 cccgcgcccg gcaggcccgg cttcaaggcc ttgccttccc gcaagggaaa agccagcacg
     1561 tccgtggtct tgtccttgcc ccgatagcgg cggttgagcc ggcgcacctc ggcgtcgccc
     1621 gtgaacacca ccgacagctc gcaacgcccc gccagggagg cgcccccggc ttcccgcagc
     1681 acccggccgg cccaacggcg ggcggcggcg gcgctcaccg gcaggcggac cccgccggcc
     1741 ctcaccagga gcctcacgcg tcggggcttt tcggcagttt ggaggcctcg tgctcgtcgt
     1801 aggcccgcac gatggactgc accagcttgt gccgcaccac gtcctgcggg ccgaaggaga
     1861 ccaccccgat gcccggcacg ccctggagca cttcccgcgc gtggatgagc ccggacgcca
     1921 ggccccgggg caggtccacc tgggtggcgt cgcccgtcac caccaccttg gacccggtgc
     1981 ccgtgcgggt gaggaacatc ttcatctgct ccgggctggt gttctgggcc tcgtccagga
     2041 tgatgaaggc gttggacagg gtgcggccgc gcatataggc caggggggcc acttccacca
     2101 cgcgctgctg gaacaggcgc tgcagcttct cgtaatccac catgtcgtag agcgcgtcat
     2161 acaaggggcg caggtaggga tccaccttgt cctgcaggtc gcccggcagg aaacccagct
     2221 tttcccccgc ctccaccgcc ggccgggtca ggaccaggcg ctccaccagc ccctcgcgca
     2281 gggccgagac cgccatggcc atggccaggt aggtcttgcc cg
//
LOCUS       DASTJJ010000275         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_773775, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000275 DASTJJ010000000
VERSION     DASTJJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773775"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="VFR02_03370"
     CDS             <1..>1112
                     /locus_tag="VFR02_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869524.1"
                     /translation="QAAPYFSAALQLDPNDLRALTGKANCESVLGQLDQALADYRRLQ
                     SIQPGDQPLADLIRQLEARTKKTPEAPPVSTALSSPPSNAFQQGLVLYNQKQFAAAVP
                     FLQGAVEENPKDPEAYQYLGLAQIQAGNLKEGDLALEEYNYLRPDPPTANYALQVRKR
                     LTPPDQMWVDARLSEAGMAPRAPGPVVQKPYGAYLVPAFGSLQLTGLETNAKSWQAFA
                     KQQQGFDSSFHFEGSVPTAALAVGLEPVLKLSKNFQVGIPFSFSPVGEARDEVSNTNK
                     VDDYVDSFNITELSLGLDLKYLIFCGGFEPFVSVAPFFAPIAIDYGAQATSGGTTQKV
                     TGNYEGDNVGAVFKLGADLHLDDTFVVTPFLGYVFSS"
BASE COUNT          205 a          391 c          335 g          181 t
ORIGIN      
        1 caggccgccc cctatttctc ggcggcgctc caactcgacc ccaacgacct gcgggccctg
       61 acgggcaagg ccaattgtga atctgtgctg gggcaattgg accaggcctt ggccgactac
      121 cggaggctgc agtccatcca accgggggac cagcccctgg ccgacctcat ccggcaattg
      181 gaggcacgga cgaaaaagac gcccgaggcg cccccggtgt cgacggccct ctcctcgccc
      241 ccgtccaacg ccttccaaca gggactggtc ctctacaacc aaaaacaatt cgccgccgcc
      301 gtccctttcc tgcagggggc cgttgaggag aaccccaagg atccggaggc ctaccaatac
      361 ctggggctgg cccagatcca agccgggaac ctgaaggaag gggacctggc cctggaggaa
      421 tacaactacc tgcgtccgga cccgccgacg gccaactacg cgctgcaagt gcgcaagcgc
      481 ctcacgcccc ccgaccagat gtgggtggac gcccgcctga gcgaagccgg catggcgccc
      541 cgggcccccg ggcccgtcgt gcaaaaaccc tacggtgcct acctggtccc ggccttcggg
      601 tccctccaac tgaccgggct ggagaccaac gccaagagct ggcaggcctt cgctaagcaa
      661 cagcagggtt tcgacagcag tttccatttc gagggctcgg tgcccacggc cgccctggcg
      721 gtggggctgg agccggtgct gaagctctcg aagaacttcc aggtggggat ccccttttcc
      781 ttctcgcccg tgggggaggc ccgggacgag gtcagcaaca cgaacaaggt cgacgactac
      841 gtcgattcat tcaacatcac cgagctttcc ctggggctgg acctgaaata cctgatcttc
      901 tgcgggggtt ttgagccctt cgtttcggtc gcccccttct tcgcccccat cgccatcgac
      961 tacggcgccc aggccaccag cggcgggacg acccaaaagg tcacgggcaa ttacgagggg
     1021 gacaacgtgg gggccgtctt caagctgggg gcggacctgc acctggacga caccttcgtg
     1081 gtgacgccct tcctgggcta cgtcttttcc tc
//
LOCUS       DASTJJ010000276         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_773905, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000276 DASTJJ010000000
VERSION     DASTJJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773905"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFR02_03375"
     CDS             complement(<1..521)
                     /locus_tag="VFR02_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9869525.1"
                     /translation="MVRGQKVVVVMPAFNAEKTLPKTYEEIPKDVVDQVILVDDASSD
                     HTPEVARTLGLTTIVHARNLGYGGNQKTCYRAALQAGADIVVMLHPDYQYTPKLVSAM
                     AYLLQSGTFDVVLGSRILGGGALSGGMPLYKYVSNRFLTLVQNLLMGAKLSEYHTGYR
                     AYTRQVLESIDWG"
     gene            complement(558..634)
                     /locus_tag="VFR02_03380"
     tRNA            complement(558..634)
                     /locus_tag="VFR02_03380"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Pro,seq:ggg)
     gene            complement(681..756)
                     /locus_tag="VFR02_03385"
     tRNA            complement(681..756)
                     /locus_tag="VFR02_03385"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(721..723),aa:Pro,seq:cgg)
     gene            complement(914..1351)
                     /locus_tag="VFR02_03390"
     CDS             complement(914..1351)
                     /locus_tag="VFR02_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9869526.1"
                     /translation="MANDEKLFYTIGEVAKLADVKPYVLRYWETEFPMLNPQKSVTGQ
                     RTYRKKDIQVALTIKRLLYEQKFTIAGAVKKLEELEKEGLDQIDLFAAKAGPEDGAPA
                     AERPSLGLEMSDGAEDGELEPDEKLMELERLIGECGEILEKYR"
     gene            complement(1393..1677)
                     /locus_tag="VFR02_03395"
     CDS             complement(1393..1677)
                     /locus_tag="VFR02_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9869527.1"
                     /translation="MTRREILKGLLALGLTESQGKKALDAFFGTLTRALERGESVSIN
                     GFGAWEWKVTRPRKARNPRTGQAVQLGSRKVLAFKPSRRLRERLNQLKKS"
     gene            complement(1674..>2353)
                     /locus_tag="VFR02_03400"
     CDS             complement(1674..>2353)
                     /locus_tag="VFR02_03400"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9869528.1"
                     /translation="MSQVLAECLPPGTRVGVLSGPSHAEEVSRKVPTTVVVASQDPDL
                     PLEIQNLFHTDYFRVYTSTDLVGVELGGALKNVIAIAAGILDGLGLGDNTKAALMTRG
                     LHEMSRLGTALGAKPETFAGLTGMGDLMVTCVSRHSRNRLLGEKIGRGAGLEAALAEM
                     TMVAEGVKTARSAVELAEGLGVEVPIMRQVHEVLYGGKGAPEAMAALLSRSAKPEREE
                     GAGGEGR"
BASE COUNT          413 a          793 c          693 g          454 t
ORIGIN      
        1 tccccccaat cgatggactc cagcacctgc cgggtatagg cccggtaacc cgtgtggtat
       61 tcggagagct tggcgcccat gagcaggttc tggaccaggg tgaggaaacg gttgctgacg
      121 tacttgtaga ggggcatgcc gcccgaaagg gccccaccgc ccaggatgcg ggaccccagg
      181 accacgtcga aggtccccga ctgcagcagg taggccatgg cggacaccag tttgggcgtg
      241 tactggtagt cggggtggag catgaccacg atgtcggcgc cggcctggag cgccgcgcgg
      301 taacaggtct tctggttgcc cccgtatccc aggttgcggg cgtgcacgat ggtggtcagg
      361 cccagggtcc gggcgacctc gggggtatgg tccgaactgg cgtcatccac caggatgacc
      421 tgatcgacca cgtccttggg gatctcttca taggttttcg gaagggtttt ttctgcgttg
      481 aaggcgggca tcacgacgac gactttttga ccgcggacca tggggatcct caaaccgggt
      541 ttgacgggaa aataaaatgg tcggggcggc aggatttgaa cctgcgacct tttgcacccc
      601 atgcaactgc gctaccgggc tgcgccacgc cccgacattt attattttcc agcgacttcc
      661 cgccatccga aatggcatca tggtcggggt gagaggattc gaacctccga ccccttggtc
      721 ccgaaccaag tgcgctagcc gctgcgctac accccgctca agataccatt tcgtggcagg
      781 aaggttccca accacccgtc attcgtctgg cgtcaaaacc gagctgctcc ccaaaaaggg
      841 aacgtcacta taaatgaaat aataggcgga ttcaacaaaa gaaaaaaggc cctcccccct
      901 gaaaaccggg ggtttagcgg tatttttcaa ggatttcgcc gcattccccg atgagccgct
      961 ccagttccat gagcttctcg tccggctcca actcgccgtc ctccgcccca tcggacatct
     1021 ccaagcccag ggacggccgc tcggccgcgg gcgccccatc ctccgggccg gccttcgcgg
     1081 cgaaaaggtc gatctggtcc aagccttctt tttccagttc ctccaatttt ttcaccgccc
     1141 ccgcgatcgt gaatttctgc tcatagagca ggcgcttgat cgtcaaagcc acctggatgt
     1201 cctttttccg gtaggtgcgc tgcccggtca cggacttctg ggggttcaac atggggaatt
     1261 cggtctccca atagcgcagc acatagggct tcacgtccgc caacttggcc acttcgccga
     1321 tggtgtaaaa aagtttctcg tcgttcgcca tgcgtctccc cgcttaatgg atcgccctat
     1381 tatccaacga aatcaggact tttttaactg atttaacctc tcccgcagcc gccggctcgg
     1441 tttgaaggcc aggaccttgc gggaccccaa ctgcaccgcc tgccccgtgc ggggattgcg
     1501 ggccttccgg ggacgggtca ccttccattc ccaggcgccg aagccgttga tggagaccga
     1561 ttccccccgc tcgagcgccc gggtcagggt cccgaaaaag gcgtccaggg ccttcttccc
     1621 ctgggactcg gtcagcccca aggccagcag gcccttcagg atctcccgcc gggtcatcgc
     1681 ccttccccgc ccgcgccttc ttcccgctcg ggcttggccg aacgggacaa aagggcggcc
     1741 atggcctccg gggccccctt gccgccgtac aggacctcgt gtacctgccg catgatcggg
     1801 acttccacgc ccaacccctc cgccaattcg accgccgacc gggccgtctt gaccccctcg
     1861 gccaccatgg tcatctcggc gagggcggct tccaggccgg ccccccggcc gatcttctcg
     1921 cccaggagcc ggttgcggct gtggcggctc acgcaggtca ccatcaggtc ccccatgccg
     1981 gtcaagcccg cgaaggtctc gggcttggcg cccaaggccg ttcccagccg gctcatctcg
     2041 tggagcccgc gggtcatcaa ggccgccttg gtgttgtccc ccaggcccag gccgtccagg
     2101 atgccggcgg cgatggcgat cacgttcttc agggcgccgc ccaattccac gcccaccagg
     2161 tcggtcgagg tatagacccg gaaataatcg gtgtggaaga ggttctggat ctccaagggc
     2221 aggtccgggt cctgggacgc caccaccacc gtggtgggga ccttgcggga cacttcctcg
     2281 gcgtggctgg gcccggacaa gacccccacc cgggtgcccg ggggcaggca ctcggccagc
     2341 acttggctca tgc
//
LOCUS       DASTJJ010000277         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_285371, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000277 DASTJJ010000000
VERSION     DASTJJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285371"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFR02_03405"
     CDS             complement(<1..587)
                     /locus_tag="VFR02_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9869529.1"
                     /translation="MLKVEHLTKMFGGLAAVNDVSVEFEAGKINAIIGPNGAGKSTFF
                     NLISGLHQPTSGRILLDGVDVTHMPSDKVARLGVGRTFQTTNLFEQASVLDNLIVGHR
                     LRTRSGLWDVLINSQRLQREEKAARDKAMEALEFVGLSHVAGEFIADITQEERKRVAF
                     ALALATDPKIVLLDEPAAGVNPEETQGFAELMRKMV"
     gene            complement(580..>1246)
                     /locus_tag="VFR02_03410"
     CDS             complement(580..>1246)
                     /locus_tag="VFR02_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9869530.1"
                     /translation="VGFIMYLLIDKWEELTHGPVGVRDIAAPYGFGLVDFTETTPFYY
                     LVLAFLVFTIWFMGRLSRSLLGRTFVAIRNGDELAQSLGINLMRNKVLAFVLSTTFAG
                     LAGALYAGMVRFIGPEDANILHTFDMITYLLVGGIGTLMGPLLGTVGIVWVTQSLQFM
                     EGYRMILFGPLLVVLVIFFPRGIAGQFLSWMHQKARTLVPAKKAKASKVETRESGEAK
                     THA"
BASE COUNT          230 a          401 c          395 g          220 t
ORIGIN      
        1 accatcttgc gcatcagctc ggcaaagccc tgggtttcct ccgggttgac cccggcggca
       61 ggctcgtcca gcagcacgat tttcggatcg gtggccaggg ccagggcgaa ggccacccgc
      121 ttgcgctctt cctgggtgat gtcggcaatg aactcgccgg ccacatggct gaggccgaca
      181 aactccagcg cctccatggc cttgtcccgg gcggctttct cctcccgttg caggcgttgg
      241 ctgttgatca gcacgtccca cagcccggag cgggtgcgca ggcggtggcc cacaatcagg
      301 ttgtccagca ccgaggcctg ctcgaacagg ttggtggtct ggaaggtacg gcccaccccc
      361 aggcgcgcca ctttgtccga cggcatgtgg gtgacgtcca ccccgtccag caggatgcgg
      421 ccggaggtgg gctggtgcag cccggaaatg aggttaaaga aggtgctttt gccggcgccg
      481 ttggggccga tgatggcgtt gatcttgccg gcttcgaact ccaccgacac gtcgttgacc
      541 gccgccaggc cgccgaacat cttggtgagg tgttccactt taagcatggg tcttggcctc
      601 cccggattcc cgtgtttcca ccttgctggc tttcgccttt ttagccggca ccagcgttct
      661 ggccttctgg tgcatccagc tcaggaactg cccggcgatg ccccggggga agaaaatcac
      721 cagcaccacc agcagcgggc cgaacaggat catccggtac ccctccatga actgcaggga
      781 ctgggtgacc cagacaatgc ccacggtgcc cagcaacgga cccatcaggg tgccgatgcc
      841 gcccaccagc aggtaggtga tcatgtcgaa ggtgtgcagg atgttggcgt cttcgggccc
      901 gatgaaccgc accatgcccg cgtacagagc gccggccaga ccggcaaagg tggtcgagag
      961 cacaaacgcc agcaccttgt tgcgcatcag gttgatgccc agggactggg ccagctcatc
     1021 gccgttgcgg atggccacga acgtgcggcc cagcagcgag cgtgacagcc ggcccatgaa
     1081 ccagatggtg aacaccagga aggccagcac caggtaataa aaaggcgtgg tctcggtgaa
     1141 gtccaccaat ccgaagccgt agggcgcggc gatgtcccgc acgcccaccg ggccgtgggt
     1201 cagctcttcc cacttgtcga tcagcaggta catgatgaag ccgacg
//
LOCUS       DASTJJ010000278         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_529854, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000278 DASTJJ010000000
VERSION     DASTJJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529854"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFR02_03415"
     CDS             <1..800
                     /locus_tag="VFR02_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9869531.1"
                     /translation="GESVAPGQSLGESYRQAVRALHLRRDRDKDLVFFAPGLVEKAEG
                     VAELDRLLADLRRRFESGAFSRLGEAVDAFLNQVLTLSFQSPEEIRWHLHYALREAGE
                     ALRNRSDLEEADFTRMARALSQAVEQAATRQEMVLAFQDGLEKLVGLSRNPGRTGDLY
                     SLEKVRRHLERNFQSPLNLARLARLAGVSPSTLSRRFKAAHGAGLETYLQDLRLQEAR
                     RLLSGGSLPLSQVARACGFKSPSYFVRFFTRKTRQSPGQYRKRAKPS"
     gene            894..1478
                     /locus_tag="VFR02_03420"
     CDS             894..1478
                     /locus_tag="VFR02_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9869532.1"
                     /translation="MGAPLDERKLVRRVLRGDERARAEFYRAYQRKLYGFCVYTLGAN
                     DPEIDDILQETFLAAFTKLPGFEFRSSLDTWLTQICIHKCYRHFRKRSRMVAQADEDL
                     EGLLAPRAREAAQDARRAEEKAGQRALVARAMPLLGEACRRILDLRSREGASYIEIGR
                     RLKVPLGTVMSRLARCTQTLRELVERLLREEGKP"
     gene            1475..2107
                     /locus_tag="VFR02_03425"
     CDS             1475..2107
                     /locus_tag="VFR02_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9869533.1"
                     /translation="MSLVDEFFKRDLNESEAQALDSLLEGSPEESARFAGLLEKEYFS
                     LGLPAPQMPSRLEVPLPGPSPLLQALAVGALLSAAAVTWWLWPAPKAALVAPRAALPA
                     ATVPHRLPALPAPPPAVPEEVTGPSPAGDRLNVVVDLDDPAPVEVDVLGAQGGPLRVL
                     YQGTLPAGRWSVKWDGRLADGSPAPAGDYRIQVLSGESRMTRTVSVAPAP"
     gene            2166..>2525
                     /locus_tag="VFR02_03430"
     CDS             2166..>2525
                     /locus_tag="VFR02_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HET9869534.1"
                     /translation="MRSHLFPRILLAAALFIPAPLFAAAPGCVGFASVDGDASQGMFL
                     AGGTVGGAGGTAVTVTTQAQFTAAATQTGPLTIVVQGLIQVSPLGQKIQVQSNKSILG
                     DGCGSGLSGGWIDLDGRS"
BASE COUNT          354 a          890 c          875 g          406 t
ORIGIN      
        1 tgggggagag cgtggccccg ggacagtccc tgggtgaatc ctaccggcag gcggtccggg
       61 ccctgcacct gcgccgggac cgggacaagg acctggtctt cttcgcgccc gggctggtgg
      121 aaaaggccga gggcgtggcc gagctggacc gcctgctcgc cgacctgcgc cggcgtttcg
      181 agtcgggcgc cttttcccgc ctgggcgagg cggtggacgc cttcttgaac caggtgctga
      241 ccctgtcctt ccaaagcccg gaggagatcc ggtggcacct gcattacgcc ctgcgggaag
      301 ccggggaggc cctccggaac cgctcggacc tggaagaagc cgacttcacc cgcatggccc
      361 gggccctcag ccaggcggtg gaacaggccg ccacgcgcca ggagatggtg ctggccttcc
      421 aggacggcct ggaaaagttg gtgggccttt cccgcaaccc cgggcgcacg ggcgacctct
      481 attcgctgga aaaggtccgg cggcacctgg agaggaattt ccaatccccc ctgaacctgg
      541 cgcggctggc ccgcctggcg ggcgtgtcgc cctccaccct cagccgccgc ttcaaggcgg
      601 cccatggcgc ggggctggaa acctatctgc aggacctgcg gctgcaggag gcccggcggc
      661 ttttgagcgg gggcagcctg cccctgtccc aggtggcccg ggcctgcggg ttcaagtcgc
      721 cttcctattt cgtccgcttc ttcacccgca agacccgcca atcccccggc caataccgaa
      781 agagggccaa accctcctaa gatcccccgc gcttggtggg gcggggttcc cggcctataa
      841 tccccgggtt cgacatttcc cggaatattc gggcccgagg ggggactaag tccatgggag
      901 cgcctttgga cgaacgcaag ctggtccgcc gggtgttgcg gggggatgag cgggcccggg
      961 ccgaatttta ccgggcctac caacggaagc tttacggttt ctgcgtctac accctggggg
     1021 ccaacgaccc ggagatcgac gacatcctcc aagagacctt cctggcggcc ttcaccaagc
     1081 tgcccgggtt cgagttccgg tccagcctgg acacctggct gacccagatc tgcatccaca
     1141 agtgctaccg gcatttccgc aagcgttccc ggatggtggc ccaggcggac gaggacctgg
     1201 agggcctgtt ggccccccgg gcccgggaag cggcgcagga tgcccggcgc gccgaggaaa
     1261 aggccggaca gcgcgccctg gtggcgcggg ccatgcccct gttgggggag gcctgccggc
     1321 ggatcctgga cctgcggtcc cgggaaggcg ccagttacat cgagatcggg cgccgcttga
     1381 aggtgcccct gggcacggtg atgtcccgcc tggcccgctg cacccagacc ctgcgggaac
     1441 tggtggaacg cctgttgagg gaagaaggga agccatgagc ctggtcgacg aatttttcaa
     1501 aagggacctg aacgaatccg aggcgcaagc cctcgactcg ctgctcgagg gctcgcccga
     1561 agaatccgcc cgcttcgccg ggttgttgga aaaggaatat ttttccctgg ggctgcccgc
     1621 gccccaaatg ccgtcccgcc tggaagtgcc gttgccggga ccttcccccc tgctccaggc
     1681 cctggcggtg ggcgccctgc tgtcggccgc cgccgtgacc tggtggctct ggccggcccc
     1741 caaggccgcg ctggtggcgc cccgggcggc ccttccggcc gccacggtcc cccatcgcct
     1801 tcccgccctt ccggcgccgc ccccggcggt gccggaggag gtcacgggcc cttcccccgc
     1861 gggcgatcgg ttgaacgtgg tggtggacct ggacgacccg gcccccgtcg aggtggacgt
     1921 gctgggggcg caaggcgggc ccctgcgggt cctttaccag ggcaccctgc ccgccggccg
     1981 ctggtccgtg aaatgggacg gccgcctggc cgacgggtcc ccggccccgg cgggggatta
     2041 ccgcatccaa gtgctctccg gggaaagccg gatgacccgc acggtgagcg tcgcccccgc
     2101 gccttgacct tccccggcgc gtgcggaggt cttccctgag tgctatgatt ttcggggagg
     2161 cacgcgtgcg ttcacacctt tttcctcgca tcctgctggc cgcggcgctt ttcatcccgg
     2221 cccccctgtt cgccgccgcc ccgggctgcg tgggtttcgc ctcggtggac ggcgacgcct
     2281 cccaggggat gttcctggcc ggcggcacgg tgggcggcgc gggcggcacg gccgtcaccg
     2341 tgaccaccca ggcccagttc accgccgcgg ccacccagac cggccccctg accatcgtgg
     2401 tgcagggcct gatccaggtc tcccccttgg gccaaaagat ccaggtgcag tccaacaagt
     2461 cgatcctggg ggacggttgc ggctcggggc tctcgggggg atggatcgac ctggacggca
     2521 ggtcc
//
LOCUS       DASTJJ010000279         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_530032, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000279 DASTJJ010000000
VERSION     DASTJJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530032"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFR02_03435"
     CDS             complement(<1..391)
                     /locus_tag="VFR02_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869535.1"
                     /translation="MIKVNLLPVGEKKKRQQLVFVALGVLFSLVVILVLGWIYMGRLQ
                     VERDLNDQIKRVDQESQGYSDKIAEIKDLEAKQASLDGFRKTLKAIADVQKTVLFAFD
                     QLAANLPGDVWITKITQGQKPDENKFVV"
     gene            complement(395..1474)
                     /gene="pilM"
                     /locus_tag="VFR02_03440"
     CDS             complement(395..1474)
                     /gene="pilM"
                     /locus_tag="VFR02_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9869536.1"
                     /translation="MVGLDLGSSTIKVVQLKQNAGKWHLAALGVADVPTEAQESKNPE
                     LQRAALLDTIKRVFKENKIKTKRVVTSLSGDAVIIRYVKLPYVTAEELRGNIAKEAEQ
                     YIPLNIDQVILDFQILGETQEEGQKKVDVLLIAAKVDVVEQHLSLLKSAGLTPTIIDV
                     DTFALSNAYEVNRLESGEETVALINIGASLSTINILEGGVTRFTRDIPVAGNDFTREI
                     QKEFNLKFSEAEELKKTHGSISMEEDDFNLSTVTHKDDRVLRMSDVMTPVLNKLLGEI
                     RRSFDYYETQSRKKTVERVVLLGGSARLKNVNRFLANKLGIPVEHFATFRNVEVEKGI
                     DPDFLGEKEFQLGVCLGLALRKAES"
BASE COUNT          349 a          528 c          466 g          383 t
ORIGIN      
        1 ccaccacgaa tttgttctcg tcgggctttt ggccctgggt gatcttggtg atccaaacat
       61 cgcccggcag gttggccgcc agttggtcaa aggcgaataa gaccgtcttc tggacatcgg
      121 cgatggcctt gagggttttc cggaagccgt ccaaactcgc ctgcttggct tccaggtcct
      181 tgatctcggc gatcttatcg ctgtaaccct gtgactcctg gtccacccgt ttgatctggt
      241 cgttcaggtc ccgctccacc tgaaggcggc ccatgtagat ccatcccaac accaggatca
      301 ccaccaagct gaagaggacc cccagcgcca caaagaccaa ttgctgccgt tttttctttt
      361 cgccgaccgg aaggaggttg actttgatca tggatcagga ctccgccttg cgcagggcca
      421 aacccaggca cacgcccaac tggaattcct tctcgcccaa gaaatcgggg tcgatgccct
      481 tttcaacctc gacattgcgg aaggtcgcga aatgctccac cgggatcccc agcttattgg
      541 ccaggaaccg gttcacgttc ttcaaccggg cgctcccgcc cagcagcacc acccgctcca
      601 cggtcttctt tcgggactgg gtctcgtagt agtcgaagga ccggcggatc tcgcccaaga
      661 gcttgttcag gaccggggtc atcacgtccg acatgcgaag gacccggtca tccttatggg
      721 tgacggtgga caagttgaaa tcgtcctctt ccatggagat ggacccatgg gtttttttca
      781 gttcttcggc ctcggagaac ttcaggttga attccttctg gatctcacgg gtaaagtcgt
      841 tcccggccac cgggatatca cgggtgaacc gggtcacgcc gccttccagg atgttgatgg
      901 tcgacaagga agccccgata ttgatgaggg cgacggtctc ttccccggat tccagccggt
      961 tgacctcata ggcgttgctc aacgcgaagg tgtccacgtc gatgatggtg ggcgtcaacc
     1021 ccgcggactt caggagcgaa aggtgctgtt ccaccacatc caccttggcc gcgatcagga
     1081 ggacgtcgac ctttttctgg ccctcttcct gggtctctcc caggatctga aagtccagga
     1141 tgacctggtc gatgttcaag ggaatgtact gttcggcttc tttcgcgata ttgccccgca
     1201 attcctcggc cgtgacgtaa ggcagcttga catagcggat gatcaccgcg tcgcccgaaa
     1261 gggaagtcac cacccgtttg gtcttgatct tgttctcttt gaagacgcgc ttgatggtgt
     1321 ccaacagggc ggcgcgctgc agttcggggt tcttgctttc ttgcgcctcc gtggggacgt
     1381 ccgccacccc caaggcggcc aaatgccatt tgccggcatt ttgcttgagt tggaccacct
     1441 tgatggtgga ggaacccaga tccaggccca ccacgttttt ccctagtaaa ccgaacattg
     1501 ctcgctcacc ttcgcccgaa tttcaaggat ttggaaagaa ccatgcccag aatgagtcca
     1561 acgctcccgg gaacatccaa aagaaaaaag cgctggcgga cagataaggc ccgaagggga
     1621 tgaatgactt ccgattcaac cggcccaccg ccatcaacgg aagggcgccc aaggccccca
     1681 ggctgaacgc caacccgatc atggcccaaa catggcttcc ccccag
//
LOCUS       DASTJJ010000280         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1508692, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000280 DASTJJ010000000
VERSION     DASTJJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508692"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFR02_03445"
     CDS             complement(<1..439)
                     /locus_tag="VFR02_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869537.1"
                     /translation="MVPVLCRECGHPLEEESRFCAVCGAPREPVERQTYEGMEAFEQG
                     EYARALTLFKAAAKKQPLGAFALRDVGHAAFHLQDDALALDYYERALQVAKGLLDVHF
                     NVGLLHMKRGHVNDAMFSFLEALQVMHPLVPGSYYLGLFHSAES"
     gene            complement(439..792)
                     /locus_tag="VFR02_03450"
     CDS             complement(439..792)
                     /locus_tag="VFR02_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869538.1"
                     /translation="MRKTMFKKEDWGLLTITALLSTFVFPPYFIHLMTMAALTEFAHL
                     WTTRLSREDREKFIEFEKEFRKGYKLEKAGRPKDAIRWYKQLEKRYAGLPQAGKLATL
                     QIRKLKGDARKGGRP"
     gene            complement(891..>2430)
                     /gene="pnp"
                     /locus_tag="VFR02_03455"
     CDS             complement(891..>2430)
                     /gene="pnp"
                     /locus_tag="VFR02_03455"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9869539.1"
                     /translation="DEESESVIFEAIKLAHKACQTLIDLQEELVKKAGKPKRALTLVK
                     PDEALVAAVKDFGLSKMKAAMAEKDKLKRQDGIAAVAKEAVEALKEKFPGKEKDIHGI
                     LHDIESDLVREAILDRQERQDGRKFDEFRPITAEVGILPRTHGSALFTRGQTQAMVVT
                     TMGTKEDQQILEELEGELKKRFMLHYNFPPYSVGEIKRLSGPGRREIGHGALAERALE
                     PLLPSFDEFPYTVQVTSDILESNGSSSMASVCGGSLSMMDAGIPLKSPCAGVALGLVS
                     NEDMSKYAILTDIQGLEDHFGDMDFKVAGTRKGITAIQMDIKIFGLPFEVVEKGLAQA
                     KQGRLTILDIMDKTLTGPREKMSQYAPRIVSIKVPTDKIRDIIGKGGATIKKIQEDYQ
                     VEVNVDDDGSVHIASANGLAIEKAKAFIDGLMAEAEEGKVYFGKVKKIMDFGAFVEIL
                     PGTDGLVHISQLDNRRVERVDDVLKEGDELLVKCIGIDPKTRKIKLSRKEALGLNIAD
                     YKKN"
BASE COUNT          379 a          770 c          782 g          499 t
ORIGIN      
        1 ggctctcggc cgagtggaac aggcccaggt aatagctgcc cggcaccagg gggtgcatga
       61 cctgcagggc ctcgaggaag gagaacatgg cgtcgttcac gtggccgcgc ttcatgtgca
      121 gcagccccac gttgaaatgc acgtccagca ggcccttggc gacctgcagg gcccgctcgt
      181 aatagtccag cgccagggcg tcgtcctgca ggtggaaggc cgcatggccc acgtcgcgca
      241 gcgcgaaggc gcccagcggc tgcttcttgg ccgccgcctt gaagagggtc agggcccggg
      301 catattcccc ctgctcgaag gcctccatac cctcataggt ctggcgttcc accggttccc
      361 ggggggcccc gcagaccgcg cagaaccggc tctcctcctc cagcgggtgc ccgcattcac
      421 ggcaaaggac cgggaccatc acgggcggcc ccctttgcgg gcgtcgccct tgagcttgcg
      481 gatctgcagc gtggccagct tgcccgcctg gggcagcccg gcgtagcgct tttccagctg
      541 cttgtaccag cggatggcgt ccttgggccg gcccgccttc tccaacttgt accccttgcg
      601 gaattccttc tcgaattcga tgaatttctc ccggtcctcc ctagaaaggc gggtggtcca
      661 gaggtgggcg aattcggtca gggccgccat ggtcatcagg tggatgaagt agggcgggaa
      721 gacgaaggtg gacaagaggg ccgtgatggt cagcaggccc cagtcctctt ttttgaacat
      781 ggtctttcgc acgggcccgg ccctccacgg cgtttaaagg cgtaaggaaa ttttacacct
      841 ataaaacaaa aaaagctccg gccttttggg ccggagcttt caggaactgc ttagttcttc
      901 ttgtaatccg cgatgttgag ccccaaggct tccttgcggg acagcttgat cttgcgggtc
      961 ttggggtcga tgccgatgca cttcaccagc agttcgtccc cttccttcaa gacgtcgtcc
     1021 acgcgctcca cccggcggtt gtccagctgg gagatgtgca ccaggccgtc ggtgcccggc
     1081 aggatctcca cgaaggcgcc gaagtccatg atcttcttca ccttgccgaa gtagaccttg
     1141 ccctcctcgg cctcggccat gagcccgtcg atgaaggcct tggccttctc gatggccaag
     1201 ccgttggcgg aggcgatgtg gaccgagccg tcgtcgtcca cgttgacctc gacctggtag
     1261 tcttcctgga tcttcttgat ggtggcgccg cccttgccga tgatgtcgcg gatcttgtcg
     1321 gtgggcacct tgatcgacac gatgcgcggc gcgtactgcg acatcttctc gcgcggcccc
     1381 gtgagggtct tgtccatgat gtccaggatg gtgaggcggc cctgcttggc ctgggccagg
     1441 cccttctcga ccacctcaaa gggcagcccg aagatcttga tgtccatctg gatggccgtg
     1501 atgcccttgc gggtgccggc caccttgaag tccatgtcac cgaagtggtc ctccaggccc
     1561 tggatgtcgg tcaggatggc gtacttggac atgtcctcgt tggacaccag gcccaaggcc
     1621 acgcccgcgc aagggctttt cagggggatg ccggcgtcca tcatggagag cgacccgccg
     1681 cagaccgagg ccatggacga ggaaccgttc gattccagga tgtcggaggt cacctgcacg
     1741 gtgtagggga actcgtcgaa ggagggcagg agcggctcca acgcgcgttc cgccagggcg
     1801 ccgtggccga tctcgcggcg gcccggaccc gacaggcgct tgatctcgcc caccgaatag
     1861 ggcgggaagt tgtagtgcag catgaagcgc ttcttcagct cgccttccag ctcttccagg
     1921 atctgctggt cttccttggt gcccatggtg gtgacgacca tggcctgggt ctggccgcgg
     1981 gtgaagagcg ccgacccatg ggtgcgtggc aggatgccca cttccgccgt gatggggcgg
     2041 aactcgtcga acttgcggcc gtcctggcgt tcctggcggt ccaagatcgc ctcgcgcacc
     2101 aggtcggact cgatgtcgtg caggatgccg tggatgtcct tttccttgcc cgggaacttc
     2161 tccttgaggg cctcgacggc ctccttggcg accgccgcga tgccgtcctg gcgcttgagc
     2221 ttgtccttct cggccatggc ggccttcatc ttggagaggc cgaagtcctt gacggccgcg
     2281 accagcgcct cgtcgggctt gaccagggtg agggcccgtt tgggcttgcc ggccttcttc
     2341 accagctctt cctgcaggtc gatgagggtc tggcaggcct tgtgggccag cttgatggcc
     2401 tcgaagatga ccgattcgga ttcctcatcc
//
LOCUS       DASTJJ010000281         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1019929, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000281 DASTJJ010000000
VERSION     DASTJJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019929"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..665
                     /locus_tag="VFR02_03460"
     CDS             75..665
                     /locus_tag="VFR02_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9869540.1"
                     /translation="MSRVCVFIDGSNLYFALKRNNKMTRVDYYQFSLALVGERRNLIR
                     TYYYNAVFDSSHFSEKAKSQQSFFDSLDRTPYLELRLGRIIQNREGHRMEKGVDVRMA
                     SDMVYYAARDFYDTAVVVTEDQDFAPAISLVKELGKHVELALFPDAQSREMVRATDKV
                     VNLDEVVNAHASLVFPPAQGAEKAQTPNDEEMAYGV"
     gene            complement(757..1731)
                     /locus_tag="VFR02_03465"
     CDS             complement(757..1731)
                     /locus_tag="VFR02_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9869541.1"
                     /translation="MIPPAPPPESRFSPSYAEPDDDPSQVEYIEMRVKTALHHTRLPY
                     SPKPFYYSLNLYRGCRHGCPYCFARYTHWFLGFQNSFDFQRKIQVKVNLLEVLDRELA
                     DPRRPKAWVTLGAATDPYQPAEKRYGLARGAFGLLAKHAWPAVFSTKSDLVLRDLDLL
                     KEVADRSNGAVAMTLTTLDPALQKFLEPHAVSIPRRLEALRRLKAAGIPCGIHLMPVI
                     PFVTDGDEALEAMVRTAKELDVDYFFHNVLRLRGAVVKGYYLSALKARDPELFQRTAR
                     LYGNRAYPDEAYRDRLRERLEGLKRKWGLRNRWKAQWKGEQLSFFDRA"
     gene            complement(1728..2207)
                     /locus_tag="VFR02_03470"
     CDS             complement(1728..2207)
                     /locus_tag="VFR02_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869542.1"
                     /translation="MSPLGTPLPVRETPAPYPAPAGRDLVYEVSLKTRAAAPWLSDLP
                     QACRLVQALTQGQLLDQYQIMDFLIWSEGFFLRVRLKAMPALADFLAFLREKTAGPGE
                     GAFAWEDELQWIRLISPEKLSESTRDFLANAEKLREGLKDRPHPSLFFFYRNRPSAQ"
     gene            complement(2246..2863)
                     /gene="lexA"
                     /locus_tag="VFR02_03475"
     CDS             complement(2246..2863)
                     /gene="lexA"
                     /locus_tag="VFR02_03475"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9869543.1"
                     /translation="MAPSEEPLTDTQRSVLKYVQDHLTQDHRPPTVREVMRHFKWNSP
                     QSARKHLLALEEKGYLEREDHTARGLRLAEDSSVGVSIPVLGRVAAGMPLLAEENRDG
                     TMVVDPSFSRGGRTLFALVVRGDSMINAHILPGDKLIVQQTTHASPGEIVVALVDGEA
                     TVKTFRKTGARVWLEPANPKYQPIIVSESQDFRIVGKVVGVFSPR"
     gene            2987..3694
                     /gene="radC"
                     /locus_tag="VFR02_03480"
     CDS             2987..3694
                     /gene="radC"
                     /locus_tag="VFR02_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET9869544.1"
                     /translation="MATLSLEKQAGSPLGPQGHRQRLRDKYARGGAPALQDYELLELL
                     LSFSIPQRDTKPYAKRLVAAFPNLKALLEADLASLRGPGGLPAQSALLIKALGDLYRL
                     AGRETFRRGTVLGSPGEAAAFFKDQLGSRPTEAFAAFFLDHRNHLLSFEVLQEGTVDQ
                     TAVYPREVLKRALDLKAGGLLVAHNHPGGSLQASEGDRQLTRQLSTAARALGITLHDH
                     LIVTAEGHFSFRQAGLL"
     gene            3709..4059
                     /locus_tag="VFR02_03485"
     CDS             3709..4059
                     /locus_tag="VFR02_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869545.1"
                     /translation="MEPTAKILEEVLPMLGAFRVEKGRWPLHAAELQAFAFSVRKPLD
                     LSWFHRLAFRPAGAERLAVDFTALPAEDFWAERGRVEVSFTLDDQEPQTLPLKARFTP
                     LGAEKVELRSFCPL"
     gene            complement(4161..4964)
                     /locus_tag="VFR02_03490"
     CDS             complement(4161..4964)
                     /locus_tag="VFR02_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET9869546.1"
                     /translation="MGLLSGKKAFIFGVANDRSIAWGIAKAFHAEGAELGFNFLGEAL
                     EKRVRPLAESVGAKLILPCDVSKDDQIEALYKEVEKAWGKFDILVHCVAFANKEELGG
                     HFYNTSRAGFHLAMDISAYSLTAITRPAVPLLNEGGSIIALTYYGSEKVIPNYNVMGV
                     AKAALEASVRYLAYDLGPQGIRVNAISAGPLKTLAASAVGGIKSMLKASEDMNPLKRN
                     ITQDEVGTTATYLASSWASGVTGEVLYVDAGANIVGMHFDEKKAEGAAS"
     gene            5126..>5336
                     /locus_tag="VFR02_03495"
     CDS             5126..>5336
                     /locus_tag="VFR02_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HET9869547.1"
                     /translation="MLKHIVMWRLKSQAEGASWEENAREMKRRLEALPALIPEILELE
                     VGLPPHAPEGQPQVVLVSAFRDEAAL"
BASE COUNT          946 a         1656 c         1739 g          995 t
ORIGIN      
        1 ggaagggtct tcggctgtaa tttaactgcc ttattcctaa aataaatccc gagaccgaaa
       61 aagaggaatg gcccatgtcc cgagtctgcg tcttcatcga tggtagcaac ctttacttcg
      121 ccctcaagcg caacaataag atgacccggg tggattatta ccaattctcc ctggccttgg
      181 tgggggagcg ccgcaacctg atccgcacct actattacaa cgcggtgttc gactcgtccc
      241 acttcagcga aaaggccaag agccagcagt ccttcttcga ctcgctggac cgcaccccct
      301 acctggaact gcgcctgggg cgcatcatcc agaaccggga agggcaccgc atggaaaagg
      361 gggtggacgt gcgcatggcc tccgacatgg tctattacgc ggcccgggat ttttacgaca
      421 ccgccgtggt ggtgaccgag gaccaggact tcgccccggc catttcgctg gtcaaggaac
      481 tgggcaagca cgtggaattg gcgctgttcc ccgacgccca aagccgggaa atggtgcggg
      541 ccaccgataa ggtggtgaac ctggacgagg tggtgaacgc ccacgcgtcc ctcgtctttc
      601 cgccggccca aggcgccgaa aaagcccaga cccccaacga cgaggaaatg gcgtacggcg
      661 tttaaggccg ggtcattccg gccctgatgg aatgaccggg acgcggaagg tcttccgccc
      721 cgcctcctgg ggcggctccc cggcgttttc ccgggcctag gcccggtcga agaaactcaa
      781 ctgctcccct ttccactgcg ccttccaacg gttccgcagg ccccatttcc gtttcaaccc
      841 ttccaggcgc tcgcgcagcc ggtcccggta agcttcatcc ggataggccc ggttgccgta
      901 gagccgggcg gtcctttgga agagctccgg gtcccgggcc ttcaaggcgg acaggtaata
      961 gcccttgacc accgcccccc gcagcctgag cacgttgtgg aaaaaataat ccacgtccag
     1021 ttccttggcg gtccgcacca tggcctccag ggcctcgtcg ccgtcggtga cgaaggggat
     1081 gacgggcatc aggtggatgc cgcaggggat gcccgcggcc ttcaggcgcc gcagggcctc
     1141 cagccggcgc gggatggaca ccgcgtgggg ctccaggaat ttttgcaagg cgggatccag
     1201 cgtggtcagg gtcatggcca cggccccgtt ggaccggtcc gccacctcct tcaacaggtc
     1261 caggtcccgc aggaccaggt ccgatttggt ggaaaagacc gcgggccagg cgtgcttggc
     1321 caaaagcccg aaggcgcccc gggccagccc gtatctcttt tcggcgggct ggtagggatc
     1381 ggtggcggcc cccagggtga cccaggcctt cggccttcgg gggtcggcca gttcccggtc
     1441 gagcacttcc aacaggttca ccttcacctg gatctttcgc tggaagtcga aggaattttg
     1501 gaagcccagg aaccaatggg tgtagcgggc gaagcagtag gggcaaccgt ggcggcagcc
     1561 ccggtagagg ttcaggctgt aatagaaggg tttggggctg taggggagcc gggtatggtg
     1621 cagggcggtc ttcacccgca tctcgatgta ttcgacctgg gacgggtcgt cgtccggttc
     1681 ggcgtaggaa ggcgagaacc ggctctccgg gggaggggcc ggggggatca ttgcgccgaa
     1741 gggcggttcc gatagaagaa gaaaagggag gggtggggac ggtctttcag cccttcccgc
     1801 agcttttcgg cgttggccag gaagtcgcgg gtggattcgg aaagtttttc cggcgagatg
     1861 aggcggatcc actggagctc gtcctcccag gcgaaggcgc cctcgcccgg gccggcggtc
     1921 ttttccctca agaaggccag gaagtcggcg agcgcgggca tggccttcag ccgcacccgc
     1981 aggaaaaatc cctcggacca gatgaggaaa tccatgatct ggtattggtc gagcagttgg
     2041 ccttgggtga gggcctgcac caggcggcag gcctggggca ggtcggacag ccagggcgcg
     2101 gccgcgcggg tcttgagcga gacctcatag accagatcgc gcccggcggg cgcgggatag
     2161 ggggccgggg tttcccggac cggaagcggc gttcctaaag ggctcatgtc caccttttca
     2221 gggacccttc cgggtccgtc gcgcctcagc gcgggctgaa gacccccacc acttttccca
     2281 cgatcctgaa atcctggctt tcggaaacga tgatgggttg gtacttgggg ttggcgggtt
     2341 ccagccagac ccgggcgccg gtcttccgga aggtcttcac ggtggcttcg ccgtccacca
     2401 aggccaccac gatctcgccc ggcgaggcat gggtcgtctg ctgcacgatc agcttgtcgc
     2461 ccggcaggat gtgggcgttg atcatcgaat cgccccggac caccagggcg aagagcgtgc
     2521 ggccgccccg ggaaaaggag gggtccacca ccatggtgcc gtcgcggttc tcctcggcca
     2581 aaagcggcat gcccgccgcc acccggccca agacggggat ggacacgccc accgaggaat
     2641 cctccgccag gcgcaggccg cgggccgtgt ggtcttcccg ttccaggtag cctttttcct
     2701 cgagcgccaa gaggtgcttg cgggcgcttt ggggtgagtt ccatttgaaa tgccgcatga
     2761 cctcccggac cgtggggggc cggtggtctt gggtcaaatg gtcctgcacg tatttcagca
     2821 ccgaccgttg ggtgtcggtc aagggttctt cggaaggcgc catggtcgtc tccttttttc
     2881 tccgcgcttg ggaggcccgg agattttcgc aaaatcgtct tgcaaagaat acgggcgtat
     2941 actagtatac gcccgtattc ctagtcaagc gaaaaaggaa cagcccgtgg ccaccctttc
     3001 ccttgaaaaa caagccggat ccccgctggg cccccaaggc caccgccaac gcctgaggga
     3061 caaatacgcc cggggcggcg ccccggccct gcaagattat gagctgctcg agcttttgct
     3121 ctctttttcc atcccccagc gggacaccaa gccctacgcc aagcgtttgg tggcggcctt
     3181 tccgaacctg aaggcgcttt tggaggcgga cctggcttcc ttaaggggtc cgggcggact
     3241 gcccgcccaa agcgcccttt tgatcaaggc cctgggcgac ctttaccggc tggcggggcg
     3301 ggaaaccttc cggcgcggga cggtcctggg cagtcccggc gaggcggcgg cttttttcaa
     3361 ggaccagttg ggcagccggc ccacggaggc cttcgcggcc ttcttcctgg accaccgcaa
     3421 ccacctgctc tccttcgagg tgcttcagga gggcacggtg gaccagaccg ccgtttatcc
     3481 ccgggaggtg ctcaagcggg cgctggacct caaggccggc gggctcttgg tggcgcacaa
     3541 ccatcccggc ggaagcctgc aggcttccga aggcgaccgg cagctgaccc gccagctttc
     3601 cacggcggcg cgggcgctgg ggatcaccct gcatgaccat ctgatcgtga ccgccgaggg
     3661 tcatttttct ttccggcaag ccggattact ttgatacagg aggacgacat ggaaccgacg
     3721 gcgaagatct tggaagaggt cctgcccatg ctgggggctt tccgggtgga aaagggccgc
     3781 tggcccctgc acgcggccga actgcaagcc ttcgcttttt cggtgcgcaa acccctcgac
     3841 ctttcctggt tccaccggct cgccttccgg cccgccggcg cggagcgcct ggcggtggat
     3901 ttcaccgccc tgcccgccga ggacttttgg gccgaacggg gccgggtgga agtttccttc
     3961 accctggacg accaggagcc ccagacgctg cccttgaagg cgcgcttcac gcccttgggc
     4021 gccgaaaagg tggagctgag gtccttttgc ccgctttgag gggaaggcgc caaaagccgg
     4081 aggggtgccg tcggggaccc cggaaacaaa aaagccgccc ttcccgggcg gcttttttgt
     4141 tttgagcggc ggggaacttt ttaagaagcg gccccttcgg ccttcttctc gtcgaagtgc
     4201 atcccgacga tgttggcgcc ggcgtccacg tacagcacct cgcccgtgac gcccgaagcc
     4261 caggaactgg ccaggtaggt ggcggtggtg cccacctcgt cctgggtgat gttgcgcttg
     4321 agggggttca tgtcctcgga ggccttgagc atgctcttga tgccgcccac ggcggaagcc
     4381 gccagggtct tgagcgggcc ggccgagatg gcgttcaccc ggatgccctg ggggcccagg
     4441 tcgtaggcca ggtaacgcac ggaggcttcc agggcggctt tggccacgcc catcacgttg
     4501 tagttgggaa tgaccttttc cgacccgtag taggtcagcg cgatgatgct gcccccttcg
     4561 ttcaagagcg gcacggcggg gcgggtgatg gcggtcaggc tgtaggccga gatgtccatg
     4621 gccaggtgga aaccggcgcg gctggtgttg tagaaatggc cgcccaattc ctctttattg
     4681 gcgaaggcca cgcaatggac caggatgtcg aacttgcccc aggccttctc cacttcctta
     4741 tataaggcct cgatctggtc gtccttggac acgtcgcagg gcaaaatgag cttggcgccc
     4801 acggactcgg ccaggggccg cacccgcttt tccagggcct cgcccaggaa attgaatccc
     4861 aattcggcgc cttcggcgtg gaaagccttg gcgatgcccc aggcgatgga gcggtcgttg
     4921 gcgaccccga agatgaaggc ctttttcccg gacaataaac ccatgcggat cctcctgatg
     4981 cgtctcgtct tggtggaaag cccgcatacc ttaacttttt ttaatgcggg tttaaagcca
     5041 aaaaagcccc ttcccccggg ggcctttgaa actatgggaa aataagcccg ctttgggtat
     5101 aaccgtcccc aaccggggag gcagcatgct caaacatatc gtcatgtgga ggctcaagtc
     5161 ccaagccgaa ggggcctctt gggaagagaa cgcccgggaa atgaaacggc gtctggaagc
     5221 cttgcccgcc cttatcccgg agatattgga actggaagtg ggcctgccgc cccatgcgcc
     5281 cgagggccaa ccccaggtgg tcctggtgtc ggctttccgg gacgaagccg cgctgg
//
LOCUS       DASTJJ010000282         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1142105, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000282 DASTJJ010000000
VERSION     DASTJJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142105"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /gene="fliN"
                     /locus_tag="VFR02_03500"
     CDS             <1..772
                     /gene="fliN"
                     /locus_tag="VFR02_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HET9869548.1"
                     /translation="LSGGVSGGGFLMTKEKDASIIADLLIGQDGTSAPEKMTELHVSA
                     FGEVVQQMGEAFAQAVQKGTGAKVSMQTGEMSAQEPAGVGAALAQLGPQVTVFSFGLY
                     SQDLLSGDVYFYLPESTAQKLGGGAVKPAATPAASVQPGAPPQATPTRSGGVVMQQVE
                     NEFSGPAKAGGNIALLMDVPLKLTVELGRTTKLVKEILALAPGSVVELDKLAGEAVDI
                     LVNEKLIAKGEVVVIDENFGVRITEIINPEERLSAVQS"
     gene            829..1485
                     /locus_tag="VFR02_03505"
     CDS             829..1485
                     /locus_tag="VFR02_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET9869549.1"
                     /translation="MRKAAPFLAVLLLAAWTALPARAVDADSPLFSQNAPASVSGQAS
                     PVSALALPEETPNPAPSFFSIVLRLFFALALTLGLIYLTVWGLKLIWEKQGWSNPVED
                     GKPLRVLGSMHLGPRKTIQVVEVGKRLLVLGVGHEEIHRLAEITDAEEIDQVRRSSLA
                     GFPHLLNRVVRRQEGGPADPGAENVFAESKQVLGGYLEKLKTMSKRKKDKDGGDSDEK
                     "
     gene            1475..2218
                     /gene="fliP"
                     /locus_tag="VFR02_03510"
     CDS             1475..2218
                     /gene="fliP"
                     /locus_tag="VFR02_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288423.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET9869550.1"
                     /translation="MKSKAWLFVLAAFLGTGAAQAADISFPTLQLGVQQAKTPQDVVM
                     SLQILLVLTVLSLAPAILIMMTSFTRIVIILSFLRQGLGTQQVPPNQVLVGLALFLTF
                     FTMHPTFDQIDKQAVTPYLQGKLSTPDAFQKAQVPLRQFMFKQTREKDLALFVALSKS
                     SRPRTPDDVSTWVLVPAFVISELKKAFEMGFVIFIPFLIIDMVTASVLMSMGMMMLPP
                     VLISLPFKILLFVMVDGWNLIVRSIALSF"
     gene            2248..2517
                     /gene="fliQ"
                     /locus_tag="VFR02_03515"
     CDS             2248..2517
                     /gene="fliQ"
                     /locus_tag="VFR02_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HET9869551.1"
                     /translation="MSVDFIADLANRALLVTLLVSAPILGLGLIVGVLVSIFQAVTQI
                     QEMTLSFIPKIIAIFVAILIFGKWMMLILLNFTSNLWINMPDYFK"
     gene            2525..3310
                     /gene="fliR"
                     /locus_tag="VFR02_03520"
     CDS             2525..3310
                     /gene="fliR"
                     /locus_tag="VFR02_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HET9869552.1"
                     /translation="MLLNPFDFSSRDVVAFILVLMRVAGLFLTSPIFSSNSIPAQVKA
                     AWTLVTAFILFPLVDYSSLDLPLLGLPFALAVVRELMVGFVVGMGAYLVFVGIQMAGQ
                     LIDIQMGFGLVNVVDPITSTQVSVMGQYYYLTAMVVFLAVDGHHLLLRGLVQTFDVIP
                     LGQAHFTPALTDKMNELFAQVFFIAFKVGAPVIGALFITNLALGIIARTIPQMNVFIV
                     GMPLNVMVGLLVVAMSMSFLVYILQDMFHGLYRDVAILIQTMR"
     gene            3318..>3583
                     /locus_tag="VFR02_03525"
     CDS             3318..>3583
                     /locus_tag="VFR02_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET9869553.1"
                     /translation="MADPAKTESATPKRRQEARERGQVVRSLEVNSVLVLIAALFTFR
                     YAGAYMMQRLADLWVFTYQNMSLGFGVENVYSYGLFYGGQIFLL"
BASE COUNT          603 a         1124 c         1109 g          747 t
ORIGIN      
        1 cctgtcgggg ggcgtgagcg gcggcgggtt cctgatgacc aaggaaaagg acgcttccat
       61 catcgcggac ctcttgatcg gccaagacgg caccagcgcg ccggagaaga tgaccgagct
      121 ccacgtgtcg gccttcggcg aggtggtcca gcagatgggg gaagccttcg cccaggccgt
      181 ccaaaagggc acgggggcca aggtgtccat gcagaccggg gaaatgtccg cccaggaacc
      241 ggcgggcgtg ggagccgccc tggcccaatt ggggccccag gtgaccgttt tttccttcgg
      301 cctctactcg caggacctcc tgtcggggga cgtttatttt tacctgcccg aatcgacggc
      361 ccaaaagctg ggcggtgggg cagtcaagcc ggcggccacg ccggccgctt cagttcaacc
      421 cggtgcgcct cctcaggcca ccccaactag gtcaggaggt gtggtgatgc aacaagtgga
      481 aaacgaattt tcgggtcccg ccaaggcggg ggggaacatc gccctgttga tggatgtccc
      541 tttgaagctg acggtcgagt tggggcgcac caccaagctg gtcaaggaga tcctggccct
      601 ggcgcccggt tccgtggtcg agctggacaa gctggcgggt gaagcggtgg acatcttggt
      661 caatgagaag ctcatcgcca agggggaggt cgtggtcatc gacgagaatt tcggcgtccg
      721 gatcaccgag atcatcaatc ccgaggaacg gttaagcgcc gttcaatcgt aagccgcccc
      781 gcccccacgc ccatggcttg cccgcccccc cgaccctgct tttggttgat gaggaaggcg
      841 gccccgttcc tggcggtcct gctgttggcg gcctggacgg ccttgcccgc ccgggcggtg
      901 gacgcggata gccccctgtt cagccagaac gccccggcct cggtctcggg ccaggcttcc
      961 ccggtctctg ccttggcctt gccggaagaa acccccaatc ccgcccccag ctttttttcg
     1021 atcgtcctgc ggttgttctt cgccctggcc ctgaccctgg gattgatcta cctgaccgtc
     1081 tggggcctca agctcatctg ggaaaagcaa ggatggagca atccagtgga ggatgggaag
     1141 cccctgcgcg tgctgggctc catgcacctg ggcccccgta agaccattca agtggtggag
     1201 gtggggaagc gcctgttggt gttgggggtg gggcacgagg agatccaccg gttggcggag
     1261 atcacggacg ccgaggagat cgaccaggtg cggcgttcca gcctggccgg gttcccccac
     1321 ttgttgaacc gggtggtgcg ccggcaggaa ggtggaccgg ccgatccggg cgcggagaac
     1381 gtcttcgccg agagcaagca ggtgctgggc ggttacctcg aaaaactgaa gaccatgtcg
     1441 aaacgcaaaa aggacaagga cggcggtgac tcggatgaaa agtaaggcat ggcttttcgt
     1501 tctcgcggcc ttcctcggca cgggggcggc ccaggcggcg gacatcagct tccccacgct
     1561 ccaattgggc gtccaacagg ccaagacgcc ccaggacgtg gtcatgagcc tgcagatcct
     1621 cttggtgctg acggtcctgt cgctggctcc ggccatcctg atcatgatga cgtccttcac
     1681 gcgcatcgtc atcatcctct cgttcctgcg ccagggcctg ggcacccagc aggtcccgcc
     1741 caaccaggtg ttggtgggtc tggccctgtt cttgaccttc ttcaccatgc atcccacctt
     1801 cgaccagatc gacaaacagg cggtcacgcc ctatctgcag ggaaagctga gcacgccgga
     1861 cgccttccag aaggcccagg tccctttgcg gcagttcatg ttcaagcaga cccgggaaaa
     1921 ggacctggcg ctcttcgtgg ccctgtccaa atccagccgt ccgcgaacgc ccgacgacgt
     1981 ttccacttgg gtgttggtgc cggccttcgt catcagcgag ctgaagaagg ccttcgagat
     2041 ggggttcgtc atcttcatcc ctttcctgat catcgacatg gtgacggcgt ccgtgctcat
     2101 gtccatgggc atgatgatgc tgccgcccgt gctgatctcg ctccccttta agatcctgct
     2161 tttcgtgatg gtggacggct ggaacctgat cgtccgctcc atcgccctga gcttttaacc
     2221 ccggccgccg gaccccaagg agcccccatg tccgttgatt tcatcgccga cctggccaac
     2281 cgggcgctcc tggtgacgct gctggtatcg gcgcccatcc tgggattggg cctcatcgtg
     2341 ggcgtgctgg tgtcgatctt ccaggcggtc acccagatcc aggaaatgac cctgtccttc
     2401 atcccgaaga tcatcgccat cttcgtggcc atcctcatct tcgggaaatg gatgatgctg
     2461 atcctgttga atttcacgtc caacctgtgg atcaacatgc ccgattactt caagtgaggc
     2521 cgctttgctc ctgaacccct ttgatttcag ctcccgggat gtggtggcct tcatcctggt
     2581 cttgatgcgg gtggcggggc tcttcctcac ctcgcccatc ttttccagca acagcatccc
     2641 ggcccaggtc aaggcggcct ggaccctggt gacggccttc atcctttttc ccctggtgga
     2701 ctacagttcg ctcgacctgc ccctgctggg gcttcccttc gccctggcgg tggtccggga
     2761 attgatggtg gggttcgtgg tggggatggg ggcctacctg gtgttcgtgg gcatccaaat
     2821 ggcgggccag ctcatcgaca tccagatggg tttcgggttg gtcaacgtgg tggaccccat
     2881 caccagcacc caggtgtcgg tcatgggcca gtactattac ctgacggcca tggtggtgtt
     2941 cctggcggtg gacggccacc acctgctgct acggggcctg gtgcagacct tcgacgtgat
     3001 ccccctggga caggcccact tcacgcccgc cttgaccgac aagatgaacg agctcttcgc
     3061 ccaggtgttc ttcatcgcct tcaaggtggg ggcgccggtc atcggggcgc ttttcatcac
     3121 caacctggcc ctgggcatca tcgcccgcac catcccccaa atgaacgtct tcatcgtggg
     3181 catgcccctg aacgtgatgg tgggcctgtt ggtggtggcc atgtcgatga gcttccttgt
     3241 gtacatcctc caggatatgt tccacggcct ctaccgggac gtggcgatcc tgatccaaac
     3301 catgcgctga gggcaccatg gccgacccgg caaaaaccga atccgcgacg cctaaacgcc
     3361 gccaggaagc ccgggaacgg ggccaggtgg tccgttcgtt ggaggtcaat tcggtcctgg
     3421 tgttgatcgc cgcgctcttc accttccgtt atgccggcgc ctacatgatg cagcgcctgg
     3481 ccgacctgtg ggtcttcacc taccagaaca tgtcgctggg tttcggggtg gagaacgtct
     3541 attcctacgg gcttttctac gggggacaga tattcctttt gat
//
LOCUS       DASTJJ010000283         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1142133, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000283 DASTJJ010000000
VERSION     DASTJJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142133"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFR02_03530"
     CDS             complement(<1..367)
                     /locus_tag="VFR02_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869554.1"
                     /translation="METMTDRFSKTAMGLLLVWMPLAYYVRSSEGFTLPKEFTGLLAL
                     GLLGGILFGSGARLLFRQPLPLTMGLFLVWMAGDAWAVAPVKSLAFAGMVHLVLAAGT
                     LWAVVLLCARGLSYARLLRY"
     gene            complement(367..>1594)
                     /locus_tag="VFR02_03535"
     CDS             complement(367..>1594)
                     /locus_tag="VFR02_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869555.1"
                     /translation="GGWTLAEPGYSRLLAEQHGFQAVSGWGWIALPWRLLMGSPDDYN
                     FAGPLALAAAPFLFFRRFRHPLLGFWARACVLYFVFGLAFTHILRFMVPVFVLFAALA
                     ACFFAGSGPRWSRGISWGAALAGGLCFFYLAAICAFYDGCAGIWWGKQTRAQYLSGPG
                     KITPYAPMARWVSENLPSDARLLVVGDARGLYYGRPFLTNSVFDEQVLAQAARGSKDA
                     FGIRERLRRLGVTHLAVNGLEGMRVGPDYGHYDLPPEAWARLDDFFKTWTQRVYDQGF
                     LQVYALRPEAQGPGPWEGEPMPLLFFTGPGRAVVEDDRRQDWGALEGDLRGALALYPG
                     CVFWHEQEALAAAGLGRGRDAEKLFVRAEAMGPLTPVGYLAWARNARSLGAAAESARV
                     MAKARRLYPALEDVQP"
BASE COUNT          325 a          563 c          522 g          184 t
ORIGIN      
        1 cgtaccgcag cagcctggcg taggaaaggc cccgcgcgca aagcaagacc accgcccaaa
       61 gcgttccggc ggccaagacc aggtgcacca tcccggcgaa ggccaggctt ttgacggggg
      121 ccaccgccca ggcgtccccc gccatccaaa ccaggaacag acccatggtc aagggcaggg
      181 gctggcgaaa caggagcctc gcgcccgacc cgaacaggat ccctcccaaa agccccaggg
      241 ccaggagacc ggtgaattcc ttgggcaggg tgaagccctc cgaggacctc acgtaatagg
      301 ccaggggcat ccagaccaaa agcaggccca tcgcggtctt ggaaaaacgg tcggtcatgg
      361 tttccatcag ggttggacgt cctcaagcgc cggatagagc cgcctggcct ttgccatcac
      421 gcgcgccgat tcggccgcgg cccccaggga ccgggcgttg cgggcccagg ccaggtagcc
      481 cacgggggtc agggggccca tggcctcggc ccggacaaaa agtttttcgg cgtcccgccc
      541 ccggcccagt cccgccgccg ccaaggcctc ttgttcatgc caaaagacgc aaccgggata
      601 aagcgccagg gccccccgca ggtccccttc cagggcgccc caatcttggc gccggtcgtc
      661 ctccaccacc gcgcggccgg ggccggtgaa gaaaagaagg ggcatgggtt ccccttccca
      721 agggcccggc ccctgggcct ccggccgcag ggcgtagacc tgcaggaaac cctggtcata
      781 gaccctttgg gtccaggtct tgaagaaatc atccaaccgc gcccaagcct ccgggggaag
      841 gtcgtaatgg ccgtagtccg gacccacccg catcccttcc aggccgttga ccgccaggtg
      901 ggtcaccccc aatcggcgca gccgctcccg gatgccgaag gcgtccttgg atccccgcgc
      961 ggcctgggcc aggacctgtt cgtcgaagac cgagttggtc aggaagggcc gtccgtaata
     1021 gagcccccgc gcgtccccca ccaccagcag gcgggcgtcg gacggcaggt tctccgagac
     1081 ccagcgggcc atgggggcgt aaggcgtgat cttgcccggc cccgacaggt attgggcccg
     1141 ggtctgcttg ccccaccaga tcccggcaca gccgtcgtaa aaggcgcaga tggcggccag
     1201 gtaaaaaaaa cagaggcccc ccgccagggc cgccccccag gaaatgcccc gggaccagcg
     1261 cgggccggag ccggcgaaga aacaggccgc cagcgcggcg aagaggacga agacggggac
     1321 catgaagcgg aggatgtggg tgaaggccag gccgaagaca aaataaagga cgcaggcgcg
     1381 ggcccagaaa cccagcaggg gatgccggaa ccgccggaag aacaggaaag gcgcggccgc
     1441 cagggccagg ggcccggcga aattatagtc atcgggggag cccatcagca gccgccaggg
     1501 cagggcgatc catccccacc cgctcaccgc ctggaaccca tgttgctcgg ccagcagccg
     1561 gctataaccc ggttcagcca gcgtccagcc cccg
//
LOCUS       DASTJJ010000284         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_530465, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000284 DASTJJ010000000
VERSION     DASTJJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530465"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VFR02_03540"
     CDS             complement(<1..1117)
                     /locus_tag="VFR02_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9869556.1"
                     /translation="MRILVIDDEKSMHVALVPFLRDGGHEVGSAFTGTEGRQKAAAFK
                     PDLVLLDLYLPDVTGLELLEGFRAGASPPAVIIMTAAAAVKTAVEAMKLGAEDYLQKP
                     LSLDEMELVLARFDKTQTLRREVAGLKDRLREEFAREFLILPDPAMQQVYGRIERVAA
                     QDKVTALLLGETGTGKEHAAKLLHALSARSAKPFVELHCGALPETLMESELFGYEAGA
                     FTGATHAKPGKFEQCNKGTILLDEIGEMPPLLQAKLLHVLQDQQFSRLGSRSVIKVDV
                     RILAATNINIPEAIASKRLREDLYYRLNAFTLQVPPLRERKEEIPILLKQFMSRMSER
                     YARPPLPLSQNLMQACLNYSWPGNLRELSNFVKRYLVL"
     gene            complement(1114..4071)
                     /locus_tag="VFR02_03545"
     CDS             complement(1114..4071)
                     /locus_tag="VFR02_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9869557.1"
                     /translation="MRPKKQEAPHFPGDELLRHLLENSLVILWAVDRDGILEFSAGKG
                     LEKLGGDPRERIGRSVFDLYRDQPAVLDNLRRALEGESFTDVVRLGGRSYEVKFVPRR
                     DAAGDPAGVLGIATDQTDRQDSAEQMDRREQYYRSLIENSHDVIYILDNEGQRVYESP
                     SVEKVLGYKPGERVGRSVYEYIHPEDHAGVRSVFEKLARAPGVAYRLELRARHKAGGW
                     RHVEVAITNLLEDPAVRGWVVNFWDITELKRATEIVEKQEQYYRALTEKTHELVLLLD
                     REAVIRYTTPSIQKILGYALADRIGKNFLELVHPEDLYRAKEIFTQTYLSTGRTMAAE
                     FRARHRDGSWRFLEASVTNLFNHPAVEGMILNARDVTERKKADMALRDRERYFRALTE
                     NAYDVVYVIDRDGKRFYESPSTERVLGYRPGDRDPTKTFEYLHPDDQEAGRAAFRRAL
                     EAPGVPVGVELRTRHKDGRWIDMEVVATNLVDVDSVRGVVVNCRDITERKRVEEEMRR
                     SEEKFRNLIENSRDVIAVVDREGRTFYVSPSIRPVMGYGPGEWIGRDFLELIHPEERE
                     AARRGFEQLKGAPGGALSAQVRARHQDGSWRLVDVSCTNLLEDPTVGGVVFNYRDVTE
                     RHAAEEALRKSEENFRSLIEKSPDAMIVHTEERIHYVNEALLWMLGYGTPAELVGRSP
                     LAVVHPDDREAVLARIRRLKPFEGYNPAQERRFTRKDGDFVDVEVVSFSILFEGRPMV
                     VAIARDLTDRKTTEQALLKYERLGAIGEMAAGLAHEIRNPLSGIQMSAEYLQKKAGLS
                     PEGVEQIRNILDQTGRLRQLVDDTLDHSRDKSARELQLVDVMSLARTSLRLAQVQYGP
                     NHNRFRVEWRVDWGRHFLRVNPYRVQQVLVNLILNAFQAMAEGGVLTLGCEKEEGWLR
                     LSVRDDGPGIPDKDLARLFEPFFTTKTSGSGLGLSVSRKIAESHGGKIRVERLKPRGT
                     VFTLELPAEGEAP"
     gene            4246..>4561
                     /locus_tag="VFR02_03550"
     CDS             4246..>4561
                     /locus_tag="VFR02_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9869558.1"
                     /translation="MRPIQVSASILSADFSDLLGSAQKAVEAGVDLLHMDVMDGHFVP
                     NISFGAPVIAGLKGKVKVPFDTHLMIENAEKYIPDFAKAGSSYILFHAEAVLDPAAVI
                     RQV"
BASE COUNT          719 a         1567 c         1447 g          828 t
ORIGIN      
        1 ctagcaccag gtagcgcttc acgaaattgc ttaactcgcg caagtttccc ggccaggagt
       61 agttcaggca cgcctgcatc agattttgtg aaagcggaag cggaggacgc gcgtaacgct
      121 ccgacatgcg cgacatgaac tgcttcagca ggatgggaat ttcttccttc cgctccctca
      181 atggcggcac ctgcagcgtg aatgcgttca ggcggtaata aaggtcttcg cgcagccgct
      241 tgctggcgat ggcttcagga atattgatgt tggtagcagc cagaattcgc acatctacct
      301 tgatgaccga gcggctgccc aatcgcgaga attgctggtc ttgaagcaca tgcagcagct
      361 tcgcctgcag cagcggcggc atttctccga tttcgtcgag cagtatcgtg cccttgttgc
      421 actgctcaaa ttttcccggc ttcgcgtgcg ttgctcccgt gaaggccccg gcctcgtagc
      481 cgaacagctc ggattccatg agggtctcgg gcagggcgcc gcaatgcagt tccacgaagg
      541 gcttggccga acgggcggag agcgcgtgca gcagcttggc cgcgtgttcc ttgcccgtgc
      601 cggtctcccc cagcaacagg gcggtcacct tgtcctgggc ggccacccgt tcgatccggc
      661 cgtagacctg ctgcatggcc ggatcgggca ggatgaggaa ctcccgggcg aactcctcgc
      721 gcaggcggtc cttgagcccc gccacctcgc ggcgcagggt ctgggtcttg tcgaagcgcg
      781 ccagcaccag ctccatctcg tccaggctca ggggcttctg caggtagtcc tccgccccca
      841 gcttcatggc ctccacggcg gtcttcacgg cggcggcggc cgtcatgatg atcaccgccg
      901 ggggggatgc cccggcgcgg aagccttcca gcagttccag gcccgtcacg tcgggcaggt
      961 acaggtccag cagcaccagg tcgggcttga aggccgccgc cttttggcgc ccctcggtgc
     1021 ccgtgaaggc cgatcccacc tcatggccgc cgtcccttaa gaaaggcacc agcgccacgt
     1081 gcatggactt ctcgtcgtcg atgaccagga tcctcatggg gcctcccctt cggcgggaag
     1141 ttccagcgtg aacaccgtgc cccggggctt gaggcgctcc acccggatct tgccaccgtg
     1201 ggactcggcg atcttccggc tcacggaaag ccccagcccc gacccgctgg tcttggtggt
     1261 gaagaagggc tcaaaaagcc tggccaggtc cttgtccggg atgcccggcc cgtcgtcccg
     1321 cacgctcagg cgcagccacc cctcctcctt ttcacaaccc aaggtcagca ccccgccctc
     1381 cgccatggct tggaaggcgt tgaggatgag gttcaccaac acctgctgga cgcggtaggg
     1441 gttcacccga aggaaatggc ggccccaatc cacccgccat tccacccgga accggttgtg
     1501 gttggggccg tactggacct gggccagccg cagcgaggtg cgggccaggg acatcacgtc
     1561 caccaactgc agttcgcgcg ccgacttgtc gcgggaatga tccagggtgt cgtccaccaa
     1621 ctggcgcagg cggcccgtct ggtccaggat gttgcggatc tgctccacgc cctcggggga
     1681 cagcccggcc ttcttttgca ggtattcggc ggacatctgg atgcccgaca gggggttgcg
     1741 gatctcgtgg gccaggcccg ccgccatctc gccgatggcc cccaggcgct cgtacttgag
     1801 cagggcctgt tcggtggtct tgcggtccgt caggtcgcgg gcgatggcca ccaccatggg
     1861 ccgcccctcg aacaggatgg aaaaactgac cacttccacg tccacgaaat ccccatcctt
     1921 gcgcgtgaag cgcctctcct gcgcggggtt gtagccttcg aagggcttga ggcggcggat
     1981 gcgggccagg acggcctccc ggtcgtcggg atggaccacc gccagggggg agcggcccac
     2041 cagttccgcg ggcgtcccat agcccagcat ccacagcagc gcctcgttga cgtaatggat
     2101 gcgctcctcg gtgtgcacga tcatggcgtc gggcgacttc tcgatgagcg agcggaaatt
     2161 ctcctcggat ttccgcaggg cctcctcggc ggcgtgccgc tcggtcacat cccggtaatt
     2221 gaagaccacg ccgcccaccg tggggtcctc caggaggttg gtgcagctca cgtccaccag
     2281 ccgccaggac ccatcctggt ggcgggcccg cacttgggcc gagagcgcgc ccccgggcgc
     2341 gcccttgagc tgctcgaatc cccgccgggc cgcctcccgt tcctcgggat ggatcaactc
     2401 gaggaaatcc cggccgatcc attccccggg tccgtagccc atgaccgggc ggatggaggg
     2461 gctcacgtag aaggtccggc cttcccggtc caccacggcg atgacgtccc gggaattctc
     2521 gatcaagttc cggaactttt cctcggaccg gcgcatttcc tcctccacgc gcttgcgctc
     2581 ggtgatgtcg cggcagttca ccaccacgcc ccgcaccgaa tccacgtcca ccaggttggt
     2641 ggccaccact tccatgtcga tccagcggcc gtccttatgc cgggtgcgca gttccacccc
     2701 cacgggcacc ccgggcgcct ccagggcccg ccggaaggcc gcccggcccg cttcctggtc
     2761 gtccggatgc aggtactcga aggtcttggt ggggtcccgg tcccccgggc ggtagcccag
     2821 cacgcgctcg gtggaggggc tttcgtagaa ccgcttcccg tcccggtcga tgacatagac
     2881 cacgtcgtag gcgttctcgg tcagcgcccg gaaataccgc tcgcggtccc gaagcgccat
     2941 gtcggccttc ttgcgttcgg tgacgtcgcg ggcgttgagg atcatgccct ccaccgccgg
     3001 gtggttgaac aggttggtga ccgaggcctc caggaaccgc caggacccgt cccggtgccg
     3061 ggcgcggaac tcggccgcca tggtgcgccc cgtggacagg taggtctggg tgaatatctc
     3121 cttggcccgg tagaggtcct cggggtggac cagttcgagg aaattcttgc cgatgcggtc
     3181 cgccagggcg tagcccagga tcttctggat cgagggcgtg gtgtagcgga tgacggcctc
     3241 ccggtccagg agcagcacca gctcatgggt cttttcggtc agggcccggt aatattgttc
     3301 ctgcttctcc acgatctcgg tggcgcgctt gagctccgtg atgtcccaaa agttgaccac
     3361 ccaaccccgc accgccgggt cctccagcag gttggtgatg gccacctcca cgtggcgcca
     3421 accgcccgcc ttgtggcggg cccgcagttc cagccggtag gccaccccgg gggcccgggc
     3481 cagcttctcg aagacgctgc gcacgccggc gtggtcctcc gggtggatgt actcgtagac
     3541 gctgcggccc acccgctcgc cgggcttgta gcccagcacc ttctccaccg aggggctctc
     3601 gtagacgcgc tgcccctcgt tgtccaggat gtagatgacg tcgtgggaat tctcgatgag
     3661 cgaccggtaa tactgctcgc ggcggtccat ctgttcggcc gaatcctgcc ggtccgtctg
     3721 gtcggtggcg atgcccagca cccccgccgg atcccccgcg gcgtcccggc gcggcacgaa
     3781 cttgacctcg taggaccggc cgcccagccg caccacgtcc gtgaaggatt caccttccag
     3841 ggcccggcgc aggttgtcca ggacggcggg ctggtcccgg taaaggtcga agaccgaccg
     3901 gccgatccgc tcccgcgggt ccccgcccaa cttttccagg cccttgcccg cggagaattc
     3961 gaggatgccg tcccggtcca cggcccacag gatcaccagg ctgttctcca gcaggtgccg
     4021 gagcagctcg tcgccgggaa aatggggggc ctcttgtttt ttcggtcgca tggcgcttct
     4081 tttaagataa ggtcggcgaa gggatggccg cgccccgggg ggcgcctagg atattggagc
     4141 tgattttgct agtatagccg caccgaccgg ggggcgccga gccttttgcg tccggcccgc
     4201 ccaatgcaac aaaaaagccc ctttccccgg gctccaagga aagccatgcg acccatccaa
     4261 gtgtccgcct ccatcctgtc ggccgacttc tcggacctgt tgggcagcgc ccaaaaggcc
     4321 gtcgaggccg gcgtggacct gctgcacatg gacgtcatgg acggccactt cgtgcccaac
     4381 atcagcttcg gggcgccggt catcgcgggg ctcaagggca aggtgaaggt gcccttcgac
     4441 acccacctga tgatcgagaa cgccgaaaag tacatccccg atttcgccaa ggcgggatcc
     4501 tcctatatcc tcttccacgc cgaggccgtg ctggacccgg cggcggtcat ccggcaggtc
     4561 c
//
LOCUS       DASTJJ010000285         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_652600, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000285 DASTJJ010000000
VERSION     DASTJJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652600"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..2375)
                     /locus_tag="VFR02_03555"
     CDS             complement(102..2375)
                     /locus_tag="VFR02_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HET9869559.1"
                     /translation="MMPQSAVLARLPGWVLGVLALSAASGLFAQTLTITSAPYNASTG
                     SADNAAAIQAAVNAVGSGGTVVVPAGTFLSGPVTLKSNMTFQLSAGATLRMTAMGTFP
                     VNTDFVYGSKLSNVAIDGSGVMDGQGQAWWDYFNGGGTDNRPPAMIELSGCTGVTVSG
                     ISVINSPKFHIQFLGNGVNILASGLTITAPWPSPNTDGIDLRGTNVNIVNCYISDGDD
                     VVQIGGSNACSGVTVSGCTFGTGHGLSIGSITSGGVSHVMVQDCVFNGTQYGLRLKSN
                     NTEGGVVSDVTYSNLTLSNILVNPILMYSYYPKVPSSPATDTGSTANSTTPYWRNIKF
                     QNVTASQAATGTANVGILWGLPQAPVSDVSFDACTLTGAKIMQVFNTQGVTFNCNCLI
                     NGKAPSNSAAVTTYNVSMAGPEDVVFAACGPTPTWTRTATPQPPTATPTRTPSPTATR
                     TKTPTPTSTFSRTPTLTATRTSTPPPPTATPTSTAPFTPTSTPSRTPTPTASPTPTAT
                     RTSTPQAFTPTPTSSPTATSSRTATPSLTPTLVLTATSTASATATASPTQTSTATASS
                     TKTLSPTPTPTPSGTPTGTPTATPVFTHTPTPTLTKTPTLSATPTATATASPTATSTP
                     TATPSPTASWTPTSTFTLPIPTGTPTPSVTQTPLPAGGQPPISRPIPYPNPAPGGVIH
                     VRFTLAAGPDDVRLRLFTTAFRKVKTQDLGPMSAGTQDIVLALTDDRGIPLANGLYYL
                     EVSDPQGTALGKLLVLR"
     gene            complement(2496..>3059)
                     /locus_tag="VFR02_03560"
     CDS             complement(2496..>3059)
                     /locus_tag="VFR02_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869560.1"
                     /translation="QAGLEGVLDGRWSLRVGYQVPLQDDQISGFAYFTAGAGLKFSSF
                     SLDYAYVPFGSLGTSHRVSLAYQFDLPQRTVPVTVIQTLPAPVTEAPVPGARDLDLEF
                     KIPAQDPALETGRQLEKEGKDAQAAQAYVEALKTDPQNNRLWGALGRLYYRLGKKDYA
                     VQCFEKELRLEPKNQPLRDWLDRYKTR"
BASE COUNT          464 a          884 c         1178 g          533 t
ORIGIN      
        1 gaccaccccc ggcgcaactg ggaccaagcc gctccaggcc tgcctctacc gcctgatcct
       61 ccttccccag gcccctttca cttggccggg gcccctttcg atcacctcaa taccagtaat
      121 ttaccgaggg cggtcccttg ggggtcggac acttccaggt aataaaggcc gttggccaag
      181 ggaatgcccc ggtcatcggt caaggcaagg acgatgtcct gggtgcccgc ggacatgggg
      241 cccaggtcct gggtcttcac tttccggaag gccgtggtga agagccgcaa acgcacgtca
      301 tccgggcccg ccgccagggt gaatcggacg tggatcacgc ctcccggcgc cgggttggga
      361 taggggatgg ggcgggaaat gggcggctgt cccccggccg ggaggggggt ctgcgtcacc
      421 gacggggtgg gggtcccggt ggggatcggc agggtgaagg tggaggtcgg cgtccaggaa
      481 gccgtgggcg acggggtcgc cgtgggcgtt gaagtcgcgg tagggcttgc agtcgctgtg
      541 gccgtggggg tggcggaaag ggtcggggtc ttcgtcaggg taggcgtggg cgtgtgggtg
      601 aagaccggcg tggccgtggg cgttccggtc ggggtgccgc tcggcgtggg ggtgggcgtg
      661 ggcgacaagg tcttcgttga tgaggcggtg gccgtggacg tctgggtggg ggacgcggtc
      721 gccgtggcgg aggcggtcga agtggcggtc aagaccaagg tcggcgtcaa ggaaggcgtc
      781 gcggtgcggg aagacgtggc cgtgggggaa gaagtcggag tgggggtaaa ggcctggggg
      841 gtggacgtgc gggtggcggt gggggtaggg ctggccgtcg gggtgggcgt cctcgaaggc
      901 gtggacgtcg gggtgaacgg cgcggtggaa gtcggggtcg ccgtgggtgg cggcggggtc
      961 gaggtgcggg tggcggtgag cgtcggggtg cggctgaagg tggaggtcgg cgtgggggtc
     1021 ttggtgcgcg tggccgtcgg ggagggcgtt cgcgtcggcg tcgccgtggg cggctggggc
     1081 gtggcggtcc gggtccaggt gggcgtgggg ccgcaggcgg cgaagaccac gtcctccggg
     1141 cccgccatgg atacgttata agtggtgacc gccgccgagt tggagggggc cttgccgttg
     1201 atgaggcagt tgcagttgaa ggtcacccct tgggtgttga agacctgcat gatcttggcg
     1261 ccggtcaagg tgcaggcgtc gaaggacacg tcggagaccg gggcctgcgg cagcccccaa
     1321 aggatgccca cgttggccgt gccggtggcg gcctggctgg ccgtcacgtt ctggaacttg
     1381 atgttccgcc aatagggcgt ggtgctgttg gccgtggacc ccgtgtcggt ggcggggctg
     1441 gagggcacct tggggtaata gctgtacatc aggatggggt tcaccaggat gttggacagg
     1501 gtcaggttcg agtaggtgac atccgacacc accccgccct cggtgttgtt ggacttgagc
     1561 cggaggccgt actgggtgcc gttgaacacg caatcctgca ccatcacgtg gctgaccccg
     1621 cccgaggtga tgctgccgat ggaaagcccg tggcccgtgc cgaaggtgca gcccgagacc
     1681 gtgacccccg agcaggcgtt ggagccgccg atctgcacca cgtcgtcgcc gtccgagata
     1741 tagcaattga cgatgttgac gttggtgccg cgcaggtcga tgccgtcggt gttgggcgaa
     1801 ggccagggcg ccgtgatggt gagccccgag gccaggatat tgaccccgtt gcccaggaat
     1861 tggatgtgga acttgggcga gttgatgacc gagatgcccg acaccgtcac gcccgtgcat
     1921 cccgaaagct cgatcatggc gggcgggcgg ttgtcggtcc cgcccccgtt gaaataatcc
     1981 caccaggcct ggccctggcc gtccatcacg cccgagccgt cgatggccac gttggagagc
     2041 ttggatccgt agacgaagtc ggtgttcacc gggaaggtgc ccatggccgt catgcgcagg
     2101 gtggcccccg ccgacaattg gaaggtcatg ttgctcttga gcgtgaccgg ccccgacagg
     2161 aaggtgccgg cgggcaccac caccgtgccg ccgctgccca ccgcgttgac ggccgcttgg
     2221 atggcggcgg cgttgtccgc gctgcccgtg gaggcgttgt agggcgccga ggtgatggtg
     2281 agggtctggg cgaaaaggcc ggaagccgcc gacagggcca ggacccctaa gacccacccc
     2341 gggaggcggg ccaaaacggc ggactgcggc atcatggacc tccaaggggc ccgcttgtca
     2401 aagcggagga tggtttcctt gacccttaga gaccccggag ggggacctta ttcccgccca
     2461 gccctttttt tcgggaaccc cgtttacccg ctcttttagc gggtcttgta ccggtccaac
     2521 cagtcccgaa gcggctggtt cttcggttcc aaccgcagtt ccttctcgaa acactggacc
     2581 gcgtaatcct ttttgcccag gcggtaatag agccggccca gggcgcccca caagcggttg
     2641 ttctgcgggt cggtcttcaa ggcctccaca taggcctggg ccgcctgggc gtctttcccc
     2701 tctttctcca actgccgccc ggtttccagc gccgggtcct gggcggggat cttgaactcc
     2761 aggtccaggt cccgcgcccc gggaacgggc gcttccgtca cgggcgccgg aagggtctgg
     2821 atgacggtca ccggcaccgt ccgctggggc aggtcgaatt gataggccag gctcacgcgg
     2881 tgcgaggtgc ccaaactgcc gaagggcaca taggcgtaat ccagggagaa ggacgagaac
     2941 ttcaggcccg ccccggcggt gaaataggcg aagccgctga tctggtcatc ctgcaagggc
     3001 acttgatagc ccacccggag ggaccaccgg ccgtccagca ccccttccag gcccgcctg
//
LOCUS       DASTJJ010000286        14630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_164231, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000286 DASTJJ010000000
VERSION     DASTJJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164231"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..474
                     /locus_tag="VFR02_03565"
     CDS             301..474
                     /locus_tag="VFR02_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869561.1"
                     /translation="MQSDGEFETTLAVLDKPETHLRIRSASGSESTPTPTDERGKHDA
                     RNVLGMGRSPEDR"
     gene            1337..1738
                     /locus_tag="VFR02_03570"
     CDS             1337..1738
                     /locus_tag="VFR02_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869562.1"
                     /translation="MGSDILAPVLGVPEGIDASGAINIRPHGIRHPLIVRRPVIRAAE
                     PSPALAMRLGIIDVFRAMLARDTVSFRGQVARMQAKVEIAHFIPPLFFTRVILIVFGF
                     ISNPPVTTLWSHQGPHDDPAGGTVPPAMDTS"
     gene            1868..3610
                     /locus_tag="VFR02_03575"
     CDS             1868..3610
                     /locus_tag="VFR02_03575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9869563.1"
                     /translation="MLVTEAGGRLALRFDYDAPAPHMPGLKLREEVRRRFPGIRWSRP
                     HGAWILPSIDNWDDILASYSPEVHPAARTLVEGIRSTFIPVKPTAAPPPDARFRMSPP
                     PFTKQVEAINFCLEQEAKGVMHNALLMDKGTGKTRTLVDLCCLLRSRGQLSRLILFCP
                     PGLQGNEAQEWGIHAWEREMGREAPEWAATILEGDGGRRLAAVARAAAAPPDTLQVLI
                     LNYEATRNALLVEALIAFAAGAPSMVACDESTCIKNPAAVQSKAVVRIGKVAARRTIM
                     TGSLIAEKPVDAFGQYLFLDDTVFGTSFVAFRARYCVMGGFESREVTGFRNMDGFRER
                     LWSRAFRCTKAECLDLPPKVGGIMNPAGPLVRSFRMPPAMAAAHRAMAERMLVEVEGR
                     RFEAPIVLTKMLRLQEITSGYVADGAAVRRLPGPNPKAELLRQELAESSDMKVIVWCR
                     EREEAYIIREAAKACGIDFFALGGATPDKHAEVEAFKAHPRRAVMAAQVRTGGMGFTI
                     TEATLAVYFSNPLSLTDREQSEDRCHRAGQSRSVTYVDLVAEGSIDVWVLALLRGKAA
                     ASADLMREELRRWK"
     gene            3627..3947
                     /locus_tag="VFR02_03580"
     CDS             3627..3947
                     /locus_tag="VFR02_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869564.1"
                     /translation="MPKVYVPHKPEKSLEYGKARAYGEIVVIFEGSVLTHNHGMVEKF
                     LKVALADFNPAEDYMVLSGSRFVIALAVALLLGRHGKVRILQYSSRNDSYEPYELDLG
                     IGSR"
     gene            4008..4424
                     /locus_tag="VFR02_03585"
     CDS             4008..4424
                     /locus_tag="VFR02_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869565.1"
                     /translation="MQGLRMLGQEWLGLWERIEKLKAEAKALEEQADELDRRMVEELQ
                     AAGERVVHLDGVGRMQLDAQLFVNVLAANKPEVLAWLKGDPEGTALVREDFSHSSLKA
                     FVKERIAAGKEIPSTVGWTPVTRIKFVPAKRQGSEA"
     gene            4484..5257
                     /locus_tag="VFR02_03590"
     CDS             4484..5257
                     /locus_tag="VFR02_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869566.1"
                     /translation="MPSQTAEQQSEKDPKDLAARPSGAIAAPAGRGLEQEVDPRDIIL
                     PRARLMQPTTPEVIEQSVPGARMGALMNSITKDILPMEFVPIVFFKNWGRFNPRKKDD
                     PHFDPAHEPGDTIWLSFDRDDPRVVKEAAWGPNGEKPAATAFLNFLAYFPGQPMPLIL
                     SFSKTSYKAGKQLLSLATMLGGDLFSHKYRITTETHKNDQGTYAVMKVDPAGAASPEE
                     QKAGEAWWAQYGKTITSVRMDDLKPEETAPTGAEEDIPF"
     gene            5319..8195
                     /locus_tag="VFR02_03595"
     CDS             5319..8195
                     /locus_tag="VFR02_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HET9869567.1"
                     /translation="MPKNDEWKNKLDYALYWSQRTDGQARGDEWTAHCPFHEDRSPSF
                     SAKLEAPGLWTCHAHCEGSGGGGSIVHFHSRLYRMSPHDAEEELRVLAGERRTVPAEE
                     VADRHRNLLGTTAVVDWLRLNKGITVETIRRFELGFDGERIWIPVKFRGHYVNVRKYD
                     WTRSDAKSKYIPYASGMNTARLFPWDNLRAEGPLYLFEGEFKAILANQLGIPGISPTG
                     GAGTWPNHFTEHARGRDVVICYDIDKEGREGAHMVAHAVMPVARSVKVVQLPITEPRN
                     GDFNDWILRGNGMDDFLDLVSRTLVFLAPKEDTWKPDIQEYRTTLSQSSNSRYQFRSV
                     DIPVTVSGKDLTPYEAPRKVTLKCGVDQGKKCSACGLRDGGLLTLDIPKTSRDILKLI
                     DCSDDDQRRVFSATARLVPCIAWTSRIEDFWPVEEIRVIPEVTFSDDDNQYVSRTIYS
                     VTEEPLKANRSYGMKGVSLPHPYTQYATVIVHEAIPSQASYEKFQMDADKAAGMAVLR
                     PAAAEGAEGRLRALTEKLGALATDLSLNVTKVTRREDILMATLLTYASVRQFSFGDEP
                     VRKGWVEACIAGDTRTAKSKTVEAVLRWLHLGEFATGENTSFAGLVGGLSQVGSRWHL
                     TWGKIPLNNGGLLVIDEAQGLSVEAIAAMSGVRSSGIAEVVKIQHERTEAQTRFIWIA
                     NPRSGRHLGTYNYGVQAVRELMGNVEDVARLDLAVTAASGEVDTTLINTLDKPKADHV
                     HTAEVMRNAILWAWSRRPNQVRFTDGAIRQVFDAANAMSRVYSSAIPLVEGGEMRIKL
                     AKLAVSAAVLSFSSEDDMTVLVREEHVLWVVGFLDLIYSKPSMGYRQFSEVQNRSRSM
                     PEEAKQRVIAEFLAFPRPGTLKETFLSVDIFQKKAFLDLVGYDTDLGKDLWTWLNRRR
                     LILTKSSGFVKTPIFTEILRRIPDGQEGGDKPDAATDTASLPAF"
     gene            8195..8638
                     /locus_tag="VFR02_03600"
     CDS             8195..8638
                     /locus_tag="VFR02_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HET9869568.1"
                     /translation="MADRLTPEQRSALMRRIKPAGTTLELWARAVVAAAWGPDLVFND
                     RSLPGSPDVAVHALKVAVFADGCFWHLCRRCVAAGRVRIPPHARWISKLEANRRRDRR
                     VRERLWRMGWRTVTLMEHEMGDAGIVEKLQGRLRGAKTNRGGQNV"
     gene            8673..9362
                     /locus_tag="VFR02_03605"
     CDS             8673..9362
                     /locus_tag="VFR02_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869569.1"
                     /translation="MKLHTDEDVIAAHERTGSMEKINWHDMVGHGGWRFCFGKPPEGS
                     VEIEGAAALPYASGLLDKMKQMAAHSDLNLRGLQSIPADLKLPGEVGGSLDLRGLQSI
                     PADLKLPGEVGGWLDLSGLQSIPADLKLPEKLGGSLYLRGLQSIPADLKLPGEVGGSL
                     DLSGLQSIPADLKLPGEVGGWLDLRGLQSIPADLKLPGEVGGWLDLSGLQSIPADLKS
                     QLNKKYAIIWP"
     gene            9359..11377
                     /locus_tag="VFR02_03610"
     CDS             9359..11377
                     /locus_tag="VFR02_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET9869570.1"
                     /translation="MIARFPARELPHRYARTEDEAVAMLEHLATRPDLVADYETTGLK
                     WHRADFRPFYVSFCDGENGWGVRLPADPDSRTHTALRRLMAMGDRRHIYHNAKFDLHA
                     GRRHRIEDLGEVHDTLLMSHLVDENRRHTLDSLGKDWLEGDVKKMSEAVDAWFAEHNM
                     GSPDKRRYDALPDELLEPYAVQDSVVAWWLWKGLEPLVRKEFAGIYEIERAALLELME
                     VESWGYKLDMDVLKRLGPSLKSSAEGTEERIRQAVKHETGKDLDEILEQPTNGHEPQG
                     SLDFGAYDTRGTGKRRFNLASQEDLARLFFSTLGIPREGLALTDNGVTSLGKYSLQKI
                     NHPVAKMIEEWRTAVKTAGSFVDNLQSLVDRDGVLHCDFQQVGTVTGRMSCQEPNLQN
                     LPKDGEVRLGFVPRPGCRLWFFDYSQIELRILAHYSQDPVMLEAFRNRRDLHRETAAV
                     LFNKGASEVTDKDRDIAKKINFTIVYGGGAGSIASQLNMGKDAAKAVLNQFFEKFPAV
                     KRFRDVATRKAEQRGWVMTHWGRKRHFPLRADLIRTGRKDKSGRRMVRWAPGHPSAGT
                     EIPEPKWYTAVNGIVSGTATGDLCKETLRRIGPRKGILRGTGAHIVSIVHDEYQVEIP
                     VEKEDTLVPAIKGAMEDYQFSVPISVDVAVSDGAWKDKHKVEVPVGAR"
     gene            11458..11787
                     /locus_tag="VFR02_03615"
     CDS             11458..11787
                     /locus_tag="VFR02_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869571.1"
                     /translation="MVNDFTSRDLYFTTEPESGSPTGDWEQSRALVRKGEEMLRNMPR
                     IGGTSWWGMGPQANRRWENWRRRVESVVERHRTLAEVDTCLGCGQYSEGGRIHNSRCR
                     QEARNPW"
     gene            11856..12212
                     /locus_tag="VFR02_03620"
     CDS             11856..12212
                     /locus_tag="VFR02_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869572.1"
                     /translation="MHGERFQENTVDLYCLKDGLFAALELKAARGLNAQKLARMATDL
                     QLADLIDVAAAGGRSALVGEDIATKVILFLDTYPAATRIQSVEALRLSLANDPTRART
                     FMSCAALVEHLFGGRR"
     gene            12209..13186
                     /locus_tag="VFR02_03625"
     CDS             12209..13186
                     /locus_tag="VFR02_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9869573.1"
                     /translation="MRCATYRNLMMVPPWCMWLSTLPNPRTRSNYEYDLRLFNHFLNE
                     RGIPGPHAAGPQDLARFMGWLLLPGTTPGGKQRDPMSARSIRRVQCSLSGFYAHMVAG
                     GMMDRDSNPTPILKTLRMRFHRQNPRPLPEVDRAALLRAMVWRTFEERRNAVAILLGF
                     HSGFRVSEVAAMRMSDMDLRDFTATQVGKGGKRRTVPLSRLLVRWLVGYMVARSKKPG
                     MHNSPWLFPSPMDVTRHISSCRLQAWLKRVARRAGIPHWDRVTVHQLRHSFCTQLAET
                     GGNAYHIMVLAGHNSIDTSQEYVNLAENSRGVREAHDRAFDRPAAAEAS"
     gene            13183..13455
                     /locus_tag="VFR02_03630"
     CDS             13183..13455
                     /locus_tag="VFR02_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9869574.1"
                     /translation="MTAHKAKLEDRRRNPLITDLRRRKGWSLQQLADAMGVSKTHACN
                     VELGNKELSPKLVGILLAKGHVDRATVEKMVQKYVRDEVFRLGKGK"
     gene            13889..14146
                     /locus_tag="VFR02_03635"
     CDS             13889..14146
                     /locus_tag="VFR02_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869575.1"
                     /translation="MRRRMSVEVPIGSVEKAVREAFDLGASGAEVRHVRLEARAVVVE
                     WEEPQSGGKTAAGIRRRLAALLRRAANVVDPGQPQGGKYGN"
     gene            14136..14483
                     /locus_tag="VFR02_03640"
     CDS             14136..14483
                     /locus_tag="VFR02_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869576.1"
                     /translation="MGTEHEKNADDQKIMRLVRQVRTFVQIAKQTVAEDGKWPTIDLT
                     PEALLDLDEVLTELTVRAGLDTAAAAQDTPQDQARICTGCYKGKMARVGALDGVILHQ
                     CPKCHMVVLMKGA"
BASE COUNT         2760 a         4752 c         4872 g         2246 t
ORIGIN      
        1 cggggagcgg gggatggggg cctgcggtcg actggcggcc gttggactcc aacgtgctgc
       61 tggccgccgg gccggagttc ccctggctgc ccgggcagcc ggtgggcatc accctcggcg
      121 tgcggttcga cctggggcta cagctgttcc cctaagaccg gctcttcacc agagacgggg
      181 gtcgaaccgt accccgaagg caagccatcc actggtggca ccggaccacg caaactcggg
      241 gccgacggac aggagtatgt tccagtccag gggccgccag tcgattgcgg gattccagcc
      301 atgcagtccg acggcgagtt cgagaccact ctggccgtcc tggacaaacc ggaaacccac
      361 ctgcgcatca ggtccgcctc cgggagcgag agtacgccaa ctcccaccga tgaacggggc
      421 aaacacgatg ccaggaacgt cctcggcatg ggccgaagtc ccgaggatcg atagtgcaac
      481 cgccgaaacc atgaatccaa acgttctcag tcttctcatg ccccacctcc ctttatgttt
      541 ttccccttcg caggggagag tctgatcttc ccttcgatcc agtcccgctg aagccggacg
      601 accaggccag tccagttgcc gccggaccgg gtggcgattc cggccgcctt gagctcggcc
      661 gcacgggcac gcatcgcatc gtgcacaacc gcgtcgaacc tatcgtcgta agtgtaggtc
      721 ctgcgtccca tcactccacc tcccgttgat ttttcccatc cccgtcctcc ggggacacca
      781 gcaccgcccc caccccaagc tgggatttgg gtatgtcggc agccagcatg ccccaccaga
      841 acgaaccttc cgtggcatcc aagggccacc tcccgaaatc caggtttccc gcaccgggcc
      901 cttgacatgg aagggcccct gggggatatt tagttcggcg ggcggaaggt ggaggaatcg
      961 aacctccgcg cccctgaaga cgctccggga tagaaaagtg tggtttttca tggtctgcct
     1021 tctgcccgat atagcccact ggctggatga ctgatagtgg attactggtt tttgcaccac
     1081 ctgacgtact ccggatcaca atatccgtat tccacggact ggagttgatc atacgaatcc
     1141 aattcatttt ggatttgttc ggacgcggtt ttcgcatcca cggagagcga cccgacgtgg
     1201 ttcctcccgt cccagttcac ggataatccg tcgaacaccc gtttcagctg cggcttcaac
     1261 tcggaaatga ttttgtttcc ggtttcgacc tttagagtca agcgcggtga aggggcctgc
     1321 attgcgtggg agctggatgg gctcggacat cctcgcgccg gtccttggcg taccggaggg
     1381 catcgatgcc tccggcgcga tcaacatccg cccacacggc atccgccatc cgctgatcgt
     1441 gcggcgcccc gtaattcggg ccgcagaacc atccccggcg ctcgccatgc ggctcggtat
     1501 aattgacgtg ttccgcgcca tgcttgcgcg cgatactgtc agctttcgcg gtcaggtggc
     1561 cagaatgcag gccaaagtgg aaatagctca ttttattcct ccattgtttt tcactcgggt
     1621 aattttgatc gtgttcggtt tcatttccaa tcctcctgta actaccttgt ggtctcatca
     1681 ggggcctcat gacgacccgg ccgggggaac ggttcccccg gccatggaca catcataggg
     1741 ctttagtgcc aaagtgtcaa ccccaacccg aaaataattt tccccgtctt tccttcccgg
     1801 ttgacaaacg ccgggttccc gtgtaccatc cgcccgttgc tcatgacatt ctgacattgg
     1861 acggaaaatg cttgtcaccg aagcaggggg gcgcctcgcc ctccggttcg actacgacgc
     1921 gcccgcgccc cacatgccgg gcctgaagct ccgcgaggag gtcaggcgcc ggttcccggg
     1981 catccgctgg tcccgccccc acggggcgtg gatcctcccc tcaatcgaca actgggacga
     2041 catcctcgcc tcctactccc cggaggtcca cccggccgcc cggaccctgg tcgaggggat
     2101 acgctcgacg ttcatccccg tcaaacccac cgccgcgccg ccccccgacg cccgcttccg
     2161 catgtcgccg ccccccttca ccaagcaggt cgaggccatc aacttctgcc tggaacagga
     2221 ggcgaagggc gtcatgcaca acgccctgct catggacaag gggaccggca agaccaggac
     2281 gctggtggac ctgtgctgcc tgctgcgctc gcgcgggcag ctctcccggc tcatcctgtt
     2341 ctgcccaccg gggctgcagg gcaacgaggc gcaggagtgg ggcatccacg cctgggaaag
     2401 ggagatggga cgggaggcgc ccgagtgggc cgccacgatc ctggaggggg acggggggag
     2461 gcgcctggcg gccgtggcca gggcggccgc cgcgcccccg gacaccctcc aggtcctaat
     2521 cctcaactac gaggccacgc gcaacgccct cctggtcgag gccctcatcg ccttcgccgc
     2581 cggggcgccc agcatggtcg cctgcgacga gtccacgtgc atcaagaacc cggcggcggt
     2641 ccagtccaag gcggtggtga ggatagggaa ggtggccgcc cggcgcacca tcatgacggg
     2701 ctcgctcatc gccgagaagc ccgtcgacgc cttcgggcag tacctgttcc tggacgacac
     2761 cgtcttcggc acgtccttcg tggccttccg ggcccggtac tgcgtcatgg gcggcttcga
     2821 gagccgggag gtcacgggct tccgcaacat ggacggcttc cgcgagaggc tctggagccg
     2881 ggccttccgc tgcaccaagg cggagtgcct ggacctgccg ccgaaggtcg gcggcatcat
     2941 gaacccggcg ggcccgctgg tgaggtcctt ccgaatgccg ccggcgatgg cggccgccca
     3001 cagggccatg gccgagcgga tgctggtgga ggtcgagggc aggcgtttcg aggcgccgat
     3061 cgtgctcacc aagatgctgc gcctgcagga gatcacctcg ggctacgtgg ccgacggcgc
     3121 ggcggtgcgg cgcctgccgg gcccgaaccc gaaggccgag ctgctcaggc aggagctggc
     3181 cgagtcctcc gacatgaaag tcatcgtctg gtgccgcgag agggaggagg cgtacatcat
     3241 ccgcgaggcc gccaaggcct gcgggatcga cttcttcgcc ctgggcggcg ccacgcccga
     3301 caagcacgcc gaggtggagg ccttcaaggc ccacccccgg cgggccgtca tggcggcgca
     3361 ggtgcgcacc ggcggcatgg gcttcacgat caccgaggcc acgcttgcgg tctacttctc
     3421 caacccgcta tcattgaccg accgcgagca gtccgaggac cggtgccacc gcgccgggca
     3481 gtcccgcagc gtcacctacg tggacctcgt cgccgagggg agcatcgacg tgtgggtgct
     3541 cgccctgctc cggggcaagg ccgccgcttc ggcggacctg atgcgcgagg agctgcggag
     3601 gtggaaatga aatgaaaggg ggaaccatgc cgaaagtata cgtcccgcac aagccggaga
     3661 agtcgctcga gtacggcaag gcccgggcct acggggagat cgtcgtcatc tttgagggga
     3721 gcgtgctcac gcacaaccac gggatggtgg agaagttcct caaggtcgcg ctggccgact
     3781 tcaacccggc ggaggactac atggtcctgt ccgggagccg cttcgtcatc gcactggccg
     3841 tggcgctcct gctggggcgc cacgggaagg tgagaatcct gcagtactcc agccgcaacg
     3901 actcctacga gccgtacgag ctggacctgg ggataggcag caggtagggc ccgaccgggc
     3961 aggaaagggg ggtggggaga atgggtagca cgacggagaa cccggccatg caggggctgc
     4021 gcatgctggg gcaggagtgg ctcggcctct gggagcggat cgagaagctg aaggcggagg
     4081 ccaaggcgct ggaggagcag gcggacgagc tcgaccggag gatggtcgag gagctgcagg
     4141 ccgccggcga gagggtcgtc cacctggacg gcgtggggcg catgcagctc gacgcccagc
     4201 tgttcgtgaa cgtcctggcc gccaacaagc cagaggtgct ggcgtggctc aagggggacc
     4261 ccgaggggac ggcgctggtc cgcgaggact tcagccactc cagcctcaag gcgttcgtga
     4321 aggagcggat cgcggcgggc aaggagatcc cgtccacggt cggctggacc ccggtgacgc
     4381 gcatcaagtt cgtgccggcg aagaggcagg gcagcgaagc ctagcaggga agcaccgcag
     4441 cacgtaacca atgaaattct gacatactga aaggagcagc accatgccta gccaaaccgc
     4501 agagcagcag tccgagaagg accccaagga cctggccgcc aggccgtccg gggcgatcgc
     4561 cgcccccgcg ggccgcggcc tcgagcagga ggtcgacccc cgggacatca tcctcccgcg
     4621 cgcgcgcctc atgcagccga cgacccccga ggtgatcgag cagtccgtcc cgggcgccag
     4681 gatgggcgcc ctgatgaact ccatcaccaa ggacatcctg cccatggagt tcgtccccat
     4741 cgtgttcttc aagaactggg gccgcttcaa cccccgcaag aaggacgacc cgcacttcga
     4801 cccggcgcac gagccgggcg acaccatctg gctgtccttc gaccgggacg acccgcgggt
     4861 ggtaaaggag gcggcctggg gccccaacgg cgagaagccg gcggccacgg ccttcctgaa
     4921 cttcctggcc tacttcccgg gccagcccat gccgctcatc ctcagcttct ccaagacctc
     4981 gtacaaggcg ggcaagcagc tgctctccct ggcgacgatg ctcggggggg acctgttctc
     5041 gcacaagtac cgcatcacca cggagaccca caagaacgac cagggcacct acgccgtgat
     5101 gaaggtcgac cccgcgggcg ccgcctcccc cgaggagcag aaggccggcg aggcctggtg
     5161 ggcgcagtac ggcaagacca tcacctccgt ccggatggac gacctcaagc ccgaggagac
     5221 cgccccgacc ggggccgagg aggacatacc cttctgaccg agccgtcaac ccaacgaaag
     5281 gaaccgcggg ggcatgggcc cctgcgggtt gacggaccat gcccaagaac gacgaatgga
     5341 aaaacaagct tgactacgcc ctctactggt cgcagcgcac cgacggacag gcgcgcggcg
     5401 acgagtggac ggcgcattgc ccgttccacg aggaccggtc cccgtccttc agcgccaagc
     5461 tggaggcccc gggcctgtgg acctgccacg cccattgcga gggcagcgga gggggcggca
     5521 gcatcgtcca cttccactcc cgcctgtacc gcatgtcgcc ccacgacgcc gaggaggagc
     5581 tcagggtcct ggccggcgag cgcaggaccg tgccggcgga ggaggtggcg gaccgccacc
     5641 gcaacctgct cgggaccacc gccgtggtgg actggctgcg gctcaacaag ggcatcaccg
     5701 ttgagacgat aaggaggttc gagctgggct tcgacgggga gcggatctgg atcccggtga
     5761 agttccgggg ccactacgtc aacgtgcgca agtacgactg gacccgcagc gacgccaagt
     5821 cgaagtacat cccctacgcc tccggcatga acacggccag gctgttcccc tgggacaacc
     5881 tcagggccga ggggccgctg tacctcttcg agggcgagtt caaggcgatc ctggccaacc
     5941 agctggggat cccggggatc tccccgacgg ggggcgccgg cacctggccg aaccacttca
     6001 cggagcacgc caggggccgc gacgtcgtca tctgctacga catcgacaag gagggccgcg
     6061 agggcgccca catggtggcc cacgccgtca tgcccgtggc caggtcggtc aaggtggtcc
     6121 agctgccgat cacggagccg cgcaacgggg acttcaacga ctggatcctc cggggcaacg
     6181 ggatggacga cttcctggac ctggtctccc gcacgctggt cttcctggcg cccaaggagg
     6241 acacctggaa gcccgacatc caggagtaca ggacgacgct ctcccagtcg tccaacagcc
     6301 gctaccagtt ccgcagcgtc gacatcccgg tgaccgtgtc cgggaaggac ctgaccccct
     6361 acgaggcccc gcgcaaggtg acgctcaagt gcggggtgga ccagggcaag aagtgcagcg
     6421 cctgcggcct gcgcgacggc ggcctgctga ccctcgacat ccccaagacc tcccgcgaca
     6481 tcctcaagct catcgactgc tccgacgacg accagcgcag ggtcttctcc gctacggcgc
     6541 gcctggtccc ctgcatcgcc tggacctccc ggatcgagga cttctggccg gtcgaggaga
     6601 tccgggtcat ccccgaggtg accttctccg acgacgacaa ccagtacgtc tcccgtacga
     6661 tctacagcgt caccgaggag cccctgaagg ccaaccgcag ctacggcatg aagggcgtct
     6721 cgctgccgca cccctacacc cagtatgcca cggtcatcgt ccacgaggcg atcccctcgc
     6781 aggcctccta cgagaagttc cagatggacg ccgataaggc ggccggcatg gccgtgctca
     6841 ggcccgcggc cgcggagggc gccgaggggc gcctgcgggc gctcaccgag aagctggggg
     6901 ccctggccac ggacctgtcg ctcaacgtga ccaaggtgac ccggcgcgag gacatcctca
     6961 tggccacgct cctcacctac gccagcgtgc ggcagttcag cttcggggac gagccggtcc
     7021 gcaagggctg ggtcgaggcc tgcatcgccg gcgacacgcg cacggcgaag tcgaagaccg
     7081 tggaagccgt cctgcggtgg ctgcacctgg gcgagttcgc gacgggggag aacaccagct
     7141 tcgccggcct ggtcggcggc ctcagccagg tgggcagccg ctggcacctg acgtggggga
     7201 agatccccct caacaacggg gggctgctcg tcatcgacga ggcccagggc ctgtccgtgg
     7261 aggcgatcgc cgccatgtcc ggggtgcgca gctccgggat cgccgaggtg gtgaagatcc
     7321 agcacgagcg caccgaggcc cagacgcgct tcatctggat cgccaacccc cgcagcggcc
     7381 gccacctggg gacctacaac tacggggtgc aggcggtccg ggagctcatg ggcaacgtcg
     7441 aggacgtcgc ccgcctggac ctggccgtga cggccgcgtc cggcgaggtc gacaccaccc
     7501 tcatcaacac cctggacaag ccgaaggccg accacgtgca cacggccgag gtcatgcgca
     7561 acgccatcct gtgggcatgg agccgacgcc ccaaccaggt gcggtttacc gacggggcca
     7621 tacgtcaggt cttcgacgcg gccaacgcca tgtccagggt ctactcgtcg gcgatcccgc
     7681 tggtggaggg aggcgagatg cgcatcaagc tggccaagct ggccgtctcc gcggcggtgc
     7741 tttccttctc cagcgaggac gacatgaccg tcctggtccg ggaggagcac gtgctgtggg
     7801 tggtcgggtt cctggacctc atctactcta agccgtcgat gggctaccgc cagttctccg
     7861 aggtgcagaa ccgcagtcgc agcatgcccg aggaggccaa gcagcgggtc atcgccgagt
     7921 tcctcgcctt cccgcggccc gggacactga aggagacgtt cctttcggtc gacatcttcc
     7981 agaagaaggc attcctggac ttggtgggct acgacaccga cctcgggaag gacctgtgga
     8041 cctggctcaa ccgccggcgg ctgatcctca ccaagtcgtc cggcttcgtc aagacgccca
     8101 tcttcacgga gatcctgcga aggatccccg atgggcagga gggaggggac aaaccggatg
     8161 ctgcgacgga tacggccagc ctgccggcct tctgatggcg gacaggctca caccggaaca
     8221 gcggtccgca ctgatgaggc gcatcaagcc cgcagggacg accctcgagc tgtgggcgag
     8281 ggcggtcgtg gccgcggcat ggggtccaga cctggtcttc aacgacagga gcctgccggg
     8341 cagccccgac gtggcggtgc acgcgctgaa ggtggccgtg ttcgcggacg gctgcttctg
     8401 gcacctttgc cggcggtgcg tggcggctgg acgtgtgcgg atcccgccac acgccaggtg
     8461 gatctccaag ctggaggcca acaggcgcag ggacaggagg gtgagggaaa gactgtggag
     8521 gatgggatgg aggacggtga cgctcatgga gcacgagatg ggcgacgccg gcatagtcga
     8581 gaagctgcag gggcgtctca ggggcgcaaa aacgaacagg ggagggcaga atgtgtgatt
     8641 ttccgagcgg atacaggttt ccagacggga cggtgaagct gcacaccgac gaggatgtga
     8701 tcgcggcgca cgagaggacg ggcagcatgg aaaagatcaa ctggcacgac atggtgggcc
     8761 acggggggtg gcgcttttgc ttcggaaaac ctcctgaagg atctgtcgag atcgaaggag
     8821 cggcggcgtt gccttacgca tccggactcc ttgacaagat gaagcagatg gcagcccatt
     8881 ccgacttgaa cctgagaggt ctccagtcga tcccggctga cctgaagctg ccgggggagg
     8941 ttggaggatc gctcgacctg agaggtctcc agtcgatccc ggctgacctg aagctgccgg
     9001 gggaggttgg aggatggctc gacctgagcg gtctccagtc gatcccggct gacctgaagc
     9061 tgccggagaa gctggggggt tcgctctacc tgagaggtct ccagtcgatc ccggctgacc
     9121 tgaagctgcc gggggaggtt ggaggatcgc tcgacctgag cggtctccag tcgatcccgg
     9181 ctgacctgaa gctgccgggg gaggttggag gatggctcga cctgagaggt ctccagtcga
     9241 tcccggctga cctgaagctg ccgggggagg ttggaggatg gctcgacctg agcggtctcc
     9301 agtcgatccc ggctgactta aagtcgcagc tcaacaagaa atacgccatc atctggccat
     9361 gatagcacga ttcccagcca gggagctacc ccacaggtac gcgcggacgg aggacgaggc
     9421 tgtggccatg ctcgagcacc tggccacgcg cccggacctg gtggccgact acgagaccac
     9481 gggcctgaaa tggcaccgcg ccgacttcag gccgttttac gtctccttct gcgacggaga
     9541 gaatggatgg ggcgtgcggc tgccggcgga tccggacagc cgcacccaca cggccctccg
     9601 ccgactgatg gccatggggg accgacgcca catctaccac aacgccaagt tcgacctaca
     9661 tgccggcagg cgccacagga tcgaggacct cggggaggtg cacgacaccc tcctgatgtc
     9721 ccacctggtt gacgagaacc ggcgacacac cctggactcg ttgggcaagg actggcttga
     9781 aggcgacgtc aagaagatgt cggaggccgt ggatgcatgg ttcgcggagc acaacatggg
     9841 cagcccggac aagcgcaggt acgacgccct tccggacgag ctgctggagc cctacgcggt
     9901 ccaggactcc gtggtggcct ggtggctctg gaagggactg gagccgctgg tccgcaagga
     9961 atttgccggc atctacgaga tcgagcgggc ggcgctgctt gagctcatgg aggtggaatc
    10021 ctggggctac aagctggaca tggacgtgct caagcgcctg ggccccagcc tcaagagctc
    10081 ggcggagggc acggaggagc gaatccgcca ggccgtgaag cacgagacgg gcaaggacct
    10141 cgacgagatc ctggagcaac ccaccaacgg gcatgaaccc caggggtcgc tggacttcgg
    10201 cgcctacgac accaggggga cggggaagag gaggttcaac ctggccagcc aggaggacct
    10261 ggccaggctg ttcttcagca cgctcgggat cccgcgggaa gggctcgcgc tcacagacaa
    10321 cggggtgacg agcctcggga agtacagcct ccagaagatc aaccacccgg tcgccaagat
    10381 gatcgaggag tggcgcacgg ccgtgaagac ggccggttcc ttcgtcgaca acctgcagag
    10441 cctcgtggac cgggacggtg tgctccactg cgatttccag caggtgggca ccgtcacggg
    10501 ccgcatgagc tgccaggagc ctaacctgca gaacctcccc aaggacgggg aggttcgcct
    10561 cgggttcgtg ccgcgacccg ggtgccggct gtggttcttc gactactccc agatcgagct
    10621 gcggatcctc gcccactaca gccaggatcc ggtcatgctc gaagccttcc ggaacaggcg
    10681 ggacctccac cgggagacgg cggccgtcct cttcaacaag ggcgcctcgg aggtcacgga
    10741 caaggatcgc gacatcgcca agaagatcaa cttcaccatc gtctacggcg gcggcgcagg
    10801 gtcgatcgcc agccagctga acatggggaa ggacgcggcc aaggccgtgc tgaaccagtt
    10861 cttcgagaag ttccccgcag tcaagaggtt ccgggacgtg gccaccagga aggcggaaca
    10921 gcggggatgg gtgatgaccc actgggggcg caagcggcac ttcccgctgc gggccgacct
    10981 gatccgtaca ggccggaaag acaagtcggg gcgcaggatg gtccgctggg cgccgggcca
    11041 tccctcggcc ggcaccgaga tcccggagcc caagtggtac acggccgtaa acgggatcgt
    11101 gtccgggacg gccacggggg acctgtgcaa ggagaccctc aggcgcatag gccccaggaa
    11161 agggatcctg agaggcacgg gcgcccatat cgtgtcgatc gtgcacgacg agtaccaggt
    11221 cgagatcccg gtcgagaagg aggacaccct cgtcccagcc atcaagggcg ccatggagga
    11281 ctaccagttc tccgtgccga tctccgtgga cgttgccgtg tcggacgggg cctggaaaga
    11341 caagcataag gtggaggtcc cggtgggcgc ccggtagtat tctttttggt tgacaaagtc
    11401 cgggggtcgt ggcacgatgt ccctgtcgct catgacattc tgagatggag gcgcctcatg
    11461 gttaacgatt tcacgtccag ggacctgtac ttcaccaccg agcccgagtc cggcagcccg
    11521 accggggatt gggaacagtc ccgcgccctg gtccgcaagg gcgaggagat gctgcggaac
    11581 atgcccagga tcgggggcac cagctggtgg ggcatgggcc cgcaggcgaa ccgccggtgg
    11641 gaaaactgga ggagacgtgt ggagtccgtc gtcgaacgtc accggaccct cgccgaggtg
    11701 gacacttgcc tgggttgcgg ccagtacagc gaaggcggac gcatccacaa cagccggtgc
    11761 cggcaggaag ccaggaaccc ctggtgacgc ccgaatctct attcaaggga cgtctacgga
    11821 agtccctgga tgacgcaggc gcctggtcca agaaaatgca cggtgaacgt ttccaggaga
    11881 acacggtcga cctctactgc ctgaaggacg gccttttcgc ggccctggaa ctcaaggccg
    11941 ctcgcggatt aaacgcacaa aaactggcaa ggatggccac cgacctgcag ttggcggacc
    12001 tcatagacgt ggcggccgcc gggggccgat ccgccctcgt gggcgaggac attgccacga
    12061 aggttatcct tttccttgac acctacccgg cggcgactag aatccagagc gtggaagcgc
    12121 tacgcctctc cctcgccaac gacccgacac gggcccgcac cttcatgtcc tgcgccgccc
    12181 ttgtggagca cctgttcggg ggacgccgat gaggtgcgcc acgtaccgca acctgatgat
    12241 ggtacccccc tggtgcatgt ggctctccac gctgcccaac ccgcgcacca ggtccaacta
    12301 cgagtatgac ctacggctgt tcaaccattt cctgaacgaa agggggatcc ccggcccgca
    12361 cgcggcgggg ccgcaggacc tggccaggtt catgggctgg ctgctcctgc cgggcacgac
    12421 ccccggcggg aagcagaggg accccatgtc agccaggagc atccgccgcg tccagtgcag
    12481 cctctccgga ttctacgccc acatggtggc cggcggaatg atggatcggg acagcaaccc
    12541 gacgccgatc ctgaagaccc tgcgcatgcg gttccaccgg caaaaccccc ggcccctgcc
    12601 ggaggtggac agggcggccc tcctcagggc catggtctgg cgcacattcg aggagcggag
    12661 aaacgccgtg gccatcctgc tgggcttcca ttcgggattc agggtgtccg aggtggccgc
    12721 catgcgtatg tccgacatgg acctgcggga cttcacggcc acgcaggtgg ggaagggagg
    12781 caagcgccgc acggtgcccc tcagccggct gctggtgcgg tggctggtgg ggtacatggt
    12841 ggcccggtcc aagaagcccg gcatgcacaa cagcccctgg ctgttcccct ccccgatgga
    12901 cgtgacccgg cacatttcat cctgtcgtct ccaggcatgg ctaaagcgtg tggcccgccg
    12961 ggcggggatc ccccactggg atagggtcac ggtgcaccag ctgcggcaca gtttttgcac
    13021 gcagctggcg gagaccgggg gcaacgcgta ccacataatg gtgctggcgg ggcacaattc
    13081 gattgacact tcccaggaat atgttaatct cgccgaaaat tcacgcggcg tgcgcgaggc
    13141 ccacgacagg gccttcgacc gccccgccgc ggcggaggcg tcatgacggc ccacaaggcg
    13201 aagctggagg acaggcggcg caacccattg atcaccgacc tgcgcaggcg caagggatgg
    13261 tcgctgcagc aactggcgga cgccatgggc gtctccaaga cccacgcctg caacgtggag
    13321 ctgggcaaca aggagctgag cccgaagctg gtggggatcc tgctggccaa gggtcatgtg
    13381 gacagggcaa ctgttgagaa aatggtgcag aagtacgtcc gtgacgaagt tttccgccta
    13441 gggaagggca aatgaccgtg gagacagccc ccatgccacc caaaattttt gccgggtaca
    13501 ttatggaatc tcctgatttc aacaatgtac tcggcaaaaa aaatgccgcc atgccccaca
    13561 tgaagcccac gtgtgcggag ctttatatat tgatgttcta taatccagta ttagggtgcg
    13621 gaagcccgga aaaatatcct gacgcacccc tgcccagccg aaaaccccat cccgagaatt
    13681 tagcccaacc gcatccagcc tcccccgcgt cccaggatat attccgggga tttttctgct
    13741 tttccgttaa accgccccaa acacaatcat ttccgctccc cgccgggaat cccgtacata
    13801 ttcccggcac ttccgaccca tccgcaaaaa actgcacaac ccgtccccgc ctccggggac
    13861 acgccacccc ctgcctcggg tgcgccgcat gaggcggcgc atgagcgtgg aggtcccgat
    13921 cggttccgtc gaaaaggcgg tccgtgaggc gttcgacctg ggtgcgtccg gcgcggaggt
    13981 gcgccacgtc cggctcgagg cgagggcggt ggtcgtggag tgggaagagc cgcagtcggg
    14041 cggaaaaaca gccgcgggga tccgcaggag gctcgcggcc ctgctgcgcc gcgccgccaa
    14101 cgtggtcgac ccggggcaac cccagggagg gaaatatggg aactgaacac gagaagaacg
    14161 cggacgacca gaagatcatg aggctcgtcc ggcaggtgcg gaccttcgtc cagatcgcca
    14221 agcagacggt tgcggaggac gggaagtggc cgacgatcga cctcacgccc gaggcgctgc
    14281 tggacctcga tgaggtcctc accgagctga cggtgcgggc ggggctcgac accgcggccg
    14341 ccgcgcagga caccccgcag gaccaggcca ggatctgcac cggctgctac aagggcaaga
    14401 tggccagggt gggcgccctc gacggcgtca tcctccacca gtgcccgaag tgccatatgg
    14461 ttgtgttgat gaagggggcc tgaccatggc gatccacagg gcggtcatcc tgcgcgcagg
    14521 cgagatgcgc gacaacgtcg tgctcccgga ggagacggtg aggggctgcg acggcgcgga
    14581 cgtggccgtg aagcgcgcgg acggcacgcc cgtccccggc gcgtcgggca
//
LOCUS       DASTJJ010000287         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_530774, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000287 DASTJJ010000000
VERSION     DASTJJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530774"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..328)
                     /locus_tag="VFR02_03645"
     CDS             complement(128..328)
                     /locus_tag="VFR02_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869577.1"
                     /translation="MFVKITGSDNCVHHINTRHITQVVESPGLWRVELSYSRGKDDLA
                     PQTIFVQNEDSKQALLAALAGS"
     gene            complement(381..1214)
                     /locus_tag="VFR02_03650"
     CDS             complement(381..1214)
                     /locus_tag="VFR02_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET9869578.1"
                     /translation="MRTKLLLCLAAAGWAGALWAEPSPDWTYARYLKAFEESGRTTHL
                     SQAQFDQVQARKLKAEDYLKQYLDSKFKKADPAILAGFARLPREYYQYNYQKGEDFSG
                     KAYEIPGRPYAIGYGSALSDYPGQAYMVQLAHPKPGDTALEIGTGSGYNASLLSTVVR
                     EAYSIEIIQPLGEAVAKIFKPLGIGNLHTRVGDGYYGWPEVKGGFDIIIVTCAAQYVP
                     PKLLEQLKPHGRMIIPIGQPFRGRQVLYVYTKDAWGKVHSRRDMGVFFIPMTGQMQKG
                     K"
     gene            complement(1211..3694)
                     /locus_tag="VFR02_03655"
     CDS             complement(1211..3694)
                     /locus_tag="VFR02_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869579.1"
                     /translation="MDPRRTLQAILHPELGERPFDWAPLFLVSAASLGFEVQLTRYFA
                     IASWSEYGYWVISIAMVGLAAGGVLLSLAKDHFRARADRVLFWIPFLLMPAAAFGFGA
                     ATWVPFNPLEFQNPDLWFGQLVNIWKYYAVLFPFFFLTGLYVGLSFLRFTDRIPSVYA
                     ADLMGAGAGSLLLMGLLYLVHPFHLLPFLLALPLGAALLGLRGLASDRRRLAWFAGAV
                     FLLSEAAAVGLNRADYCQYKAVYPPLHVAGNKILGQVLSPRGQYLLLDNFTERVDLDL
                     SNNYGLLKVDGPPPAYGLYLDGNRITQLPKALPVDLRYTYATLDVLPYFLRQHSRALL
                     VGTRGGFKVREALSHEGMILDALEPDPVLYRMVDRERLDLEQDLRTDIHLYNQTPLGF
                     LRGHPGLYDLVDISPDFLGQADANRFAFTQEALRRYYGCLSVRGILVIPASIREFTVY
                     ALKEEESAYEMLRSLGVPDPEKHLVLYRSAWSTRLLVFKTPPSDADLHLLKKFCFRRS
                     FDISYEAGRPIPRGQVWNELPPLPFEEAAGEPDETEDSLRDDSRKLLSKGHDAFLKGY
                     FFNLRPSTLDHPFFYATLRLSRLGEALSNITLIPREEIGTLVNLAVLVQSLALALGVL
                     SLPLLFRRRLGGRGAGWLALYFAALGLGFLFIEIVLIEKASYFLGDRATAFTLVLAGM
                     LLFSGGGSAWAGRLAADRARWGLTRALGGLGLWLLAALAGLAPLLDVSLSWPTPLKCL
                     LLLAVLAPAAFALGVPFALGLGRLGGRPEWVPWAWALNGAFSVVATPLAALLAVGWGY
                     GPLLWLSLGLYAAAGWAFGKIGGNLEAVS"
     gene            complement(3704..>3937)
                     /locus_tag="VFR02_03660"
     CDS             complement(3704..>3937)
                     /locus_tag="VFR02_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869580.1"
                     /translation="GHPGAWRPDYGRVQIPVLCLTSESYHSQGLWLKSRLRGMELETW
                     ASDGHALFADDPAAFNEKLRAFLGSIGFPGAPE"
BASE COUNT          760 a         1285 c         1328 g          564 t
ORIGIN      
        1 tcctcccgcc aaggccacgc cgccgccaag atgctgatcc tgcggtgacc gcccaataaa
       61 aaaggccggg ccccttgggc ccggcctttt ttattggcgc tgcgcttcag ctcatccggg
      121 ccctagctca cgaccccgcc agggcggcca gcaaagcctg cttggaatcc tcgttctgca
      181 cgaagatggt ctggggcgcc aggtcgtcct tgccccggga ataggagagc tccacccgcc
      241 acaggccggg ggattccacc acctgggtga tgtggcgcgt gttgatgtgg tgcacgcaat
      301 tatccgaacc cgtgatcttc acgaacatgg ggacctcctc ccggacggtt tccgtccatt
      361 gtacccccgg atccccggga tcacttcccc ttttgcatct gtcccgtcat ggggatgaag
      421 aaaacgccca tgtcgcggcg cgaatgcacc ttgccccagg cgtccttggt gtagacatag
      481 agcacctgcc ggccccggaa gggctggccg atggggatga tcatgcggcc gtggggcttg
      541 agctgctcca agagcttggg cggcacgtac tgggccgcgc aggtgacgat gatgatgtcg
      601 aagcccccct tcacctcggg ccaaccgtaa tagccgtccc ccacccgggt gtgcaggttc
      661 ccgatgccca ggggcttgaa gatcttggcg accgcctcgc ccaggggctg gatgatctcg
      721 atggaatagg cctcccggac cacggtggac agcaaggacg cgttgtagcc gctgcccgtg
      781 ccgatctcca gggccgtgtc gccgggcttg ggatgggcca attggaccat gtaggcctgg
      841 cccgggtagt cgctcagggc cgagccgtaa ccgatggcat agggccgtcc ggggatctca
      901 taggccttgc cggaaaaatc ctcgcccttc tggtagttgt actggtagta ctcgcggggc
      961 agccgggcga agcccgccag gatcgcgggg tcggccttct tgaacttgga atccaggtat
     1021 tgtttcaggt aatcctcggc cttgagcttg cgggcctgca cctggtcgaa ttgggcctgg
     1081 ctcaaatggg tggtgcggcc gctttcctcg aaggccttga ggtagcgcgc gtaggtccaa
     1141 tcgggcgagg gctcggccca cagggctccg gcccatccgg ccgccgccag gcacaacagc
     1201 aatttggtcc tcatgacacc gcctccaagt tcccgccgat ttttccgaaa gcccacccag
     1261 ccgccgcgta aagccccagg ctcaaccaca acagcgggcc gtagccccag ccgacggcca
     1321 agagggcggc cagcggcgtg gccaccaccg aaaaggcgcc gttcaaggcc caggcccaag
     1381 gcacccattc gggccggccc cccaggcggc ccaaccccag ggcgaagggc acccccaacg
     1441 cgaaggccgc gggcgccagg accgccagca agagcaggca ttttaagggc gtgggccagg
     1501 aaagggaaac atccaaaagc ggggccaacc cggccagcgc cgccagcagc cacagcccca
     1561 agccgcccag ggcccgggtc aggccccatc gcgcccggtc cgccgccagg cgcccggccc
     1621 aggcgctgcc cccgccggag aaaaggagca tccccgccag caccagcgtg aaggccgtgg
     1681 cccggtcccc gaggaaatag gaggccttct cgatgagcac gatctcgatg aagaggaagc
     1741 ccaggcccaa ggcggcgaaa taaagggcca gccatcccgc gccccgcccc cccaaccggc
     1801 ggcggaagag caggggcagc gacaacacgc ccagggccag cgccagggac tggaccagca
     1861 cggccaggtt gaccagggtg ccgatctctt cccgggggat gagggtgatg ttggacaggg
     1921 cctcccccag ccgggaaagg cgcagggtcg cgtaaaagaa ggggtggtcc agggtggaag
     1981 gcctcaggtt gaagaaataa cccttgagga aggcgtcgtg gcccttggac aggagcttgc
     2041 ggctgtcgtc gcgcagggaa tcctcggtct catcgggctc gcccgcggcc tcctcgaagg
     2101 gcaggggcgg cagctcgttc cagacctggc cccgggggat gggccgcccc gcctcatagg
     2161 aaatgtcgaa ggaccgtcgg aagcagaact tcttcagcag atggaggtcc gcgtccgaag
     2221 gcggcgtctt gaagaccaaa agccgggtgc tccaggccga gcggtacaac accaggtgtt
     2281 tttccggatc cggcacgccg agggagcgga gcatctcata agcgctttct tcctccttca
     2341 gggcgtagac cgtgaactcg cggatggagg cggggatcac caggatgccc cggacgctca
     2401 ggcacccgta gtagcgccgc agggcctcct gggtgaaggc gaagcggttg gcgtccgcct
     2461 ggcccaggaa atcgggggag atgtccacca ggtcgtagag ccccggatgc ccgcgcagga
     2521 accccagggg ggtctggttg tacaggtgga tgtcggtccg caggtcctgt tccaggtcca
     2581 ggcgttcccg gtccaccatg cggtaaagga ccggatcggg ttccaacgcg tccaggatca
     2641 tgccctcatg ggacaaggcc tcgcggacct tgaaaccacc ccgggtgccc accaacagcg
     2701 cgcgcgaatg ctggcgcagg aaataaggca gcacgtccag cgtggcgtag gtgtagcgca
     2761 ggtccaccgg cagggccttg ggcagctggg tgatgcggtt gccgtccagg tacaagccgt
     2821 aggcgggcgg cggcccgtcc accttgagca ggccgtagtt gttcgaaagg tccaggtcca
     2881 cccgttcggt gaaattgtcc aacagcaggt attgcccccg gggcgacaac acctggccca
     2941 ggatcttgtt gcccgccacg tgcagcgggg ggtagacggc cttgtattgg caatagtccg
     3001 cccggttgag ccccaccgca gcggcctcgc tgagcaggaa caccgcgccc gcgaaccagg
     3061 ccaggcgccg cctatccgag gcgagcccgc gcaggcccaa cagggccgcc cccaggggca
     3121 gggccaagag gaagggcagc aggtggaagg gatgcaccag gtacagcagg cccatgagca
     3181 acagcgaccc ggcgcccgcg cccatcaggt ccgccgcgta gaccgagggg atgcggtccg
     3241 tgaagcgcag gaaggacagg cccacgtaga ggcccgtgag gaaaaagaag gggaagagga
     3301 cggcgtaata cttccagatg ttcaccaact ggccgaacca aaggtccggg ttctggaatt
     3361 ccagcgggtt gaagggcacc caagtggccg cgccgaaccc gaaggccgcc gccggcatca
     3421 gcaggaaggg gatccaaaaa agcacccggt ccgcgcgcgc gcggaaatgg tccttggcca
     3481 ggctcaacaa caccccgccc gccgccaggc ccaccatggc gatggagatg acccagtaac
     3541 cgtactcgga ccaactggcg atggcgaaat aacgggtgag ctgcacctcg aaccccaggg
     3601 aggccgcgga gaccaggaac aggggcgccc agtcgaaggg cctctccccc aactcggggt
     3661 ggaggatggc ctggagcgta cggcgcgggt ccaaggaggt tccttactcg ggggcccccg
     3721 ggaagccgat gcttcccagg aaggcccgca gtttttcatt gaaggcggcg gggtcgtcgg
     3781 caaagagggc gtggccgtcc gacgcccagg tctccaactc catcccgcgc aggcgggact
     3841 tcagccagag gccctgggaa tgatagcttt ccgaggtcaa acaaagaacc ggaatctgga
     3901 cgcggccgta gtcgggccgc caggccccgg ggtggcc
//
LOCUS       DASTJJ010000288         1095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1020799, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000288 DASTJJ010000000
VERSION     DASTJJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020799"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..842)
                     /gene="csb2"
                     /locus_tag="VFR02_03665"
     CDS             complement(75..842)
                     /gene="csb2"
                     /locus_tag="VFR02_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HET9869581.1"
                     /translation="MEETNPITTARFKLYGKPLPRVEEALAVGEALRAAAMGRAKRLL
                     GPEAIPPELSGHRPERLDGHGHAFWLPEPDNKGEISHVLVHVPGGLGPESIRVLSALG
                     FMRQGEGEPLRLVLEGLGKAGLFASESGLTRESAVWRSVTPYLYPWHLKKPQTRTPEA
                     LRQAVLDQLRREWQGRGKEPSEIVDFKELPDLSFGGRRLRPLVFRRFRRKKGLVQPDR
                     LGRFMEVCFKAPVRGPLALGFACHFGLGLFEPVSGES"
BASE COUNT          230 a          377 c          295 g          193 t
ORIGIN      
        1 cccaacaggt ccgaaaaggc cacattttga agggctccct gggaaagccg ggccagtaat
       61 tttcgccggt tcattcaact ttccccggaa accggttcga acaaacccag cccgaaatgg
      121 caggcgaacc ccagggccaa aggaccccgc acgggggcct tgaaacacac ttccatgaac
      181 cggcccaggc gatccggctg gaccaggcct ttcttccggc ggaaccgccg gaaaaccaat
      241 ggccgcaacc ggcggccccc aaacgagagg tcgggcagtt ccttgaaatc cacgatctcg
      301 gacggttctt ttccccggcc ctgccactcc cggcgcaatt ggtccaaaac cgcctggcgc
      361 agggcctccg gcgtccgtgt ctggggtttc ttcaaatgcc agggataaag gtagggcgtc
      421 acgctccgcc agacggccga ctctcgggtc aatcctgatt cggacgcgaa cagtccggcc
      481 ttgcccagcc cctccaatac cagccggagc ggctccccct ccccctggcg catgaacccc
      541 agggccgaaa gcacccggat ggattccggt cccagccctc ctggaacgtg gaccaagaca
      601 tggctgattt cgcctttatt atccggttcg ggcagccaaa aagcgtgccc gtggccgtca
      661 agacgctccg gccgatggcc ggaaagttcc ggggggatgg cttcgggccc caacaaccgc
      721 ttggcgcgcc ccattgccgc cgcccgcagg gcttccccca cggccagcgc ctcctccacc
      781 cggggaagcg gtttgccgta gagcttgaac ctcgcggtgg tgattggatt ggtctcttcc
      841 atacgaaacc ttgggcttgg attcaccgcc atggtcttca ggacctcaag cggcctgcgg
      901 taggtcggag acgaactcca tcccgccaat cttaacgcac ccgtttccat tataaactcc
      961 tcaccggcgc cataattccc agccccagga aacggccatt acccaatatc aacgggcctg
     1021 aaacgttttc cgaaaaggaa atctcaacat gccacaggca ttcctttgga aaacgagtcc
     1081 cgggtgaaaa tgcct
//
LOCUS       DASTJJ010000289         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1632327, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000289 DASTJJ010000000
VERSION     DASTJJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632327"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VFR02_03670"
     CDS             <1..1074
                     /locus_tag="VFR02_03670"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9869582.1"
                     /translation="QMIELIQGLEKGGHAYAVPSTDLGRDVLYDSASFAAYGRLSGKD
                     LEQLLEGARVEKDERKKRPTDFVLWKASKPGEPQWDSPWGKGRPGWHIECSAMVRRHL
                     GDSIDIHGGGVDLIFPHHENEIAQCEALTGKPYVKYWLHNGFIEIAGQKMSKSLGNIW
                     KAKDALAQFKPPVLRYFFLSAHYRSGLNYDRPILEAAARGWEEFNLTFQRIEEALNRP
                     DEGVVGNLLELRLALDEVEGKFRDAMDDDFNTPKAMAALFELARLVRQTLSQPGASHP
                     GAKSLLGEAGNKLAEIGGLLGIVSRERASIPPEVEQWAQERTRLKVEKKFKEADELRA
                     RIQEKGFTIEDVPGGRFRLLPKV"
     gene            1124..2539
                     /locus_tag="VFR02_03675"
     CDS             1124..2539
                     /locus_tag="VFR02_03675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET9869583.1"
                     /translation="MSFVSFSFLFLFALSLGARAWIGPRKNEKAYLILLMAFSLVFYG
                     AFIPRYLWILFFTTGVDYWAGWRIHRAPGLREKRPWLVLSLTANLGLLAYFKYSGFAV
                     DSLRSLCASLGWGADWVPRLSPALPIGISFFTLQSMSYTLDLYRGKLEPVRGYWRFLL
                     FVCFYTHLVSGPIVRAKELLYQFDRRRRIRAAVWMQGAYLMILGFFLKIVVADHLAEY
                     VDRYWVRGGAPQMPASVPWALAFLFSCQIFADFFGYTNIALGAAYLLGFKLPRNFNNP
                     YLAGSFREFWTRWHITLSRWIRDYLYLPLGGGRGGAGRAAFALMITFLLAGLWHGAAL
                     TFLVWGAFHGLALLLERGLGFRNMEGRSPLLRAGWFLVVQLGILVSWVFFRASDIPQG
                     FRFLANMTGPSFAWGDLLRILPAFLFAAPVLVLHVRGFLAERGWFPAPGGREKAVWAA
                     AMLYAVLTLYGKNNAFIYFQF"
     gene            2565..>3245
                     /locus_tag="VFR02_03680"
     CDS             2565..>3245
                     /locus_tag="VFR02_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869584.1"
                     /translation="MAGRLALFAGAFLFLAALGLFLASHLKGDRVWRGRCFDLPEKGG
                     ILDEVDSDHFDGYDRLYYGIGPAVENARRADILLLGNSRVLFAFRAPAVRAFEQKSGL
                     KVFNFCFPANDGLVMAWEAIQRNDLRPSLVVVNENQFFDLGRSPYGTGTVEEGRWGAW
                     TRVWEDGMSWDARCWLHRFFPRFGFGDLYPAQPTVTVQSSRDGCLLYEDFSRGRSVYP
                     LHGTGAWDH"
BASE COUNT          489 a         1014 c         1067 g          675 t
ORIGIN      
        1 cagatgatcg aactcatcca gggcctggaa aagggcggcc atgcctacgc ggtcccctcc
       61 accgacctgg gccgggacgt tctttatgat tcggcctctt tcgccgccta cggccggctg
      121 tccggcaagg acctggaaca gctgttggaa ggcgcccggg tggaaaagga cgagcgcaag
      181 aagcgcccga cggatttcgt gctctggaag gcctccaaac ccggggaacc ccaatgggat
      241 tcgccctggg ggaaggggcg gccggggtgg cacatcgagt gttccgccat ggtgcgccgc
      301 cacctggggg actccatcga catccatggc ggcggggtgg acctcatttt cccccaccat
      361 gagaacgaga tcgcccagtg cgaggccctg accggcaagc cctacgtgaa atactggctg
      421 cacaacggtt tcatcgagat cgcgggccag aagatgagca aatcgctggg gaacatctgg
      481 aaggccaagg acgcgttggc ccagttcaag ccccccgtgc tgcgctactt cttcttgagc
      541 gcccattacc gcagcggcct caattatgac cgtcccatcc tggaggccgc ggcgcggggc
      601 tgggaagagt tcaacctgac cttccagcgc atcgaggaag ccttgaaccg cccggacgag
      661 ggggtggtgg ggaacctttt ggaactgcgc ctggccttgg acgaggtcga gggaaaattc
      721 cgcgatgcca tggacgacga cttcaacacg cccaaggcca tggcggcctt gttcgagttg
      781 gcccgcctgg tgcgccagac cctgagccag cctggggcct cccacccggg agccaagagc
      841 ctgctggggg aagcggggaa caagctggcc gagatcggcg gattgctcgg catcgtgtcc
      901 cgggaacggg ccagcatccc gcccgaggtg gagcagtggg cccaggagcg gacccggctg
      961 aaggttgaaa agaaattcaa agaggccgac gaactgcggg cccgcatcca ggaaaagggc
     1021 ttcaccatcg aggatgttcc aggcggccgg ttccggctgc tgcccaaggt atgacggaac
     1081 gacgggccct tcgggccggt cccgccgccg gaggatcccg gccatgagct tcgtttcctt
     1141 ttccttcctt ttcctcttcg ccctgagcct gggcgcgcgg gcctggatcg gtccccggaa
     1201 gaatgaaaag gcctacctga tcctgctgat ggccttcagc ctggtcttct atggcgcctt
     1261 catcccgcgg tacctctgga tattgttctt caccaccggc gtggactatt gggccgggtg
     1321 gcggatccat cgggcccccg gcctccggga aaaaaggccc tggttggtgc tttccctgac
     1381 ggccaatttg ggcctgctgg cctatttcaa gtattcaggg ttcgcggtgg attccctgag
     1441 gagcctctgc gcctccctcg ggtggggggc ggattgggtg ccccgactgt cgcccgccct
     1501 gccgatcggc atctccttct tcaccctcca aagcatgagc tacacgctgg acctttaccg
     1561 gggcaagttg gaacccgtcc ggggctattg gcggttcctg ctcttcgtgt gcttctatac
     1621 ccacttggtc tcgggcccca ttgtgcgggc caaggagctg ttgtaccagt tcgaccgtcg
     1681 gcgccggatc cgggccgcgg tctggatgca gggcgcctac ctgatgatcc tgggtttttt
     1741 cctgaagatc gtggtggcgg accacctggc cgaatacgtg gaccgttatt gggtccgggg
     1801 gggcgccccc cagatgccgg cttcggtgcc ttgggccctg gccttcctgt tctcctgcca
     1861 gatcttcgcg gacttcttcg gctataccaa catcgccctg ggcgcggcct atctattggg
     1921 tttcaagctc ccgcggaact tcaacaaccc ctacctggcg ggctccttcc gggaattctg
     1981 gacccgctgg cacatcacgc tgtcccgctg gatccgggat tatctctact tgcccctggg
     2041 cggcggccgg ggcggcgcgg gccgcgccgc cttcgcgctc atgatcacct tcctattggc
     2101 gggtctttgg catggggcgg ccctcacttt cctggtatgg ggcgcctttc acggcttggc
     2161 cttgttgctg gaaagagggc tgggtttccg gaacatggaa gggaggtccc cgctgttgcg
     2221 ggcgggatgg ttcctggtgg tgcagcttgg gatattggtc tcttgggtct tcttccgggc
     2281 ctccgacatt ccccaagggt tccgcttttt ggccaacatg acgggacctt ccttcgcctg
     2341 gggggacctg ctgcgcatcc tgccggcctt ccttttcgcc gccccggtcc tggtcctgca
     2401 tgtccgggga ttcttggccg aaaggggttg gttccccgcg ccggggggcc gggaaaaggc
     2461 ggtttgggcc gcggccatgt tgtatgccgt actgacgctt tatgggaaga acaatgcctt
     2521 catttatttc caattttaag ggccggtcgg cccgggaacg ccgtttggcg gggcggttgg
     2581 cccttttcgc gggggctttc ctgttccttg cggcgctggg cctgttcctg gcctcccacc
     2641 tgaaagggga ccgggtctgg cggggccggt gtttcgacct tcctgaaaaa ggcggcatcc
     2701 tggacgaggt cgacagcgac cactttgacg ggtatgaccg cctctattac gggatcggcc
     2761 cggccgtgga gaacgcccgc cgggcggaca tccttcttct ggggaattcc cgggtgcttt
     2821 tcgccttccg ggcgcccgcc gtccgggctt tcgaacagaa gagcgggctc aaggtcttca
     2881 acttttgctt ccccgccaat gacggcctgg tcatggcctg ggaggccatc cagcgcaacg
     2941 acttgaggcc ttccctggtg gtggtcaatg agaaccagtt cttcgatctg ggccgcagcc
     3001 cctatgggac cgggaccgtg gaggaggggc gatggggggc ctggacccgg gtctgggaag
     3061 atgggatgtc gtgggacgcg cggtgctggc tgcaccgctt tttcccccgg ttcggctttg
     3121 gggaccttta cccggcccag cccacggtca cggtccaatc ctcccgggac ggctgcctgc
     3181 tgtacgagga cttctcccgg ggccgttcgg tttatcccct ccacgggacc ggggcctggg
     3241 accat
//
LOCUS       DASTJJ010000290         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_775694, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000290 DASTJJ010000000
VERSION     DASTJJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775694"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFR02_03685"
     CDS             complement(<1..446)
                     /locus_tag="VFR02_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9869585.1"
                     /translation="MASRISIEIQGLYKKFGAVQALDGVFARFEPARLHGVIGPDGAG
                     KTTLLRHLMGLLKADRGKVRFRDRDRTLAFEALRPRMAYMPQQQSLYADLSVSEHLDF
                     FRDLYQIPAALYARRRDELLSITRLGPFTARPAGQLSGGMYKKLGL"
     gene            complement(455..1195)
                     /locus_tag="VFR02_03690"
     CDS             complement(455..1195)
                     /locus_tag="VFR02_03690"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9869586.1"
                     /translation="MKKKLIPAILLALLAAGGLLTWHFWKPAPFAYAGTVEATEVDVP
                     ARVASTIASLAVDEGRPVTQGQLLASLDGQDYRLAQRLADGDYTRSLALYQSGSIPAE
                     TFQHTQIQKQQADLKVQWCSILSPLTGVVLTKYHEPGEWVNPGTRLFTLADLSQVWCL
                     IFVPQPDLARLSYGMKLSAFLPELPGKAFGGEVTHINDQAEFTPKNVQTEKERTHLVY
                     AVKVTFPNPDGLLKPGMTLEMTLPPGPK"
     gene            complement(1192..1851)
                     /locus_tag="VFR02_03695"
     CDS             complement(1192..1851)
                     /locus_tag="VFR02_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9869587.1"
                     /translation="MARPSKNTDQRLIQAAQELLRDSSFSRLGLRQVAARAGVNPGMF
                     HYHFKTKDQFLRAVLQDGYEKFFKDFSLRVEGEEKPLDKLRQALFALARFARDNRRFG
                     LTLLREVLDGNREVRDFLWKNVPRHGKVMVQLIRRCQKEGSLARMPLPQAAAFLMPAV
                     AAAAFGVAGLESQAPSLLARGLFKGLGALVLSDAAIRKRVDMALHGLGADNNGPTRRR
                     A"
BASE COUNT          321 a          593 c          714 g          336 t
ORIGIN      
        1 atgaggccca gtttcttgta catgccgccc gacaattggc ccgcgggccg cgcggtgaag
       61 gggccgagcc gggtgatgga aagcagttcg tcccggcgcc gggcatagag cgcggcgggg
      121 atctggtaaa ggtcgcggaa aaagtccagg tgttcggaca ccgacaggtc ggcgtagagg
      181 ctttgttgct ggggcatgta ggccatgcgg ggccgcaggg cctcgaaggc cagggtccgg
      241 tcccggtccc ggaagcggac ctttccccgg tcggccttca gcaggcccat caggtggcgc
      301 agcagggtgg tcttgcccgc gccgtcgggg ccgatgacgc cgtgcaggcg cgcgggctcg
      361 aaacgcgcga agaccccgtc cagggcctgg acggccccga acttcttgta aagaccttgg
      421 atctcgatgg aaatccgcga cgccatggcg ggccttattt cgggccgggg ggcagggtca
      481 tctccagggt cataccgggc ttgaggagac cgtccgggtt ggggaaggtg acctttaccg
      541 cgtagaccag gtgggtcctt tccttctcgg tctgcacgtt cttgggcgtg aattcggcct
      601 ggtcgttgat gtgggtgact tcgcccccga aggccttgcc gggaagctcg ggcaggaagg
      661 cggagagctt catcccataa gacaggcgcg ccaggtcggg ttggggcacg aagatgaggc
      721 accagacttg gctgaggtcc gccagggtga aaaggcgggt gcccgggttc acccattcgc
      781 cgggctcatg gtatttggtc aacacgaccc cggtgagggg ggagaggatg gagcaccatt
      841 gcaccttgag gtcggcctgt tgtttttgga tctgcgtgtg ctggaaggtc tccgcgggga
      901 tggagccgct ttggtacagg gccaggctgc gggtgtaatc gccgtcggcc agccgctggg
      961 ccagccggta atcctggccg tccagggagg ccaacaactg cccctgggtc acgggccggc
     1021 cctcgtccac ggccagggaa gcgatggtgg aggcgacccg ggcggggaca tccacctcgg
     1081 tggcttccac cgtgcccgcg taggcgaagg gggcgggctt ccaaaaatgc caggtgagca
     1141 ggccgccggc cgccaagagg gccagcagga tggctgggat gagtttcttt ttcatgcgcg
     1201 tctccttgtc ggtccgttgt tatccgcccc cagcccatgg agggccatgt ccacccgctt
     1261 gcggatggcc gcgtcggaca ggaccagggc gcccagtccc ttgaaaagcc cccgggccaa
     1321 gagcgagggg gcctggcttt ccagccccgc cacgccgaaa gccgccgccg ccacggcggg
     1381 catcaggaag gccgccgcct gcgggagcgg catgcgcgcg aggctccctt ccttctggca
     1441 gcggcggatg agctggacca tcaccttccc gtgccggggc acgttcttcc agaggaaatc
     1501 ccggacctcc cggttcccgt ccaggacctc ccgcagcagg gtcaggccga aacgccggtt
     1561 gtcccgggcg aaacgggcca gcgcgaaaag ggcctggcgc agcttgtcca ggggcttttc
     1621 ctcgccctcc acccgcaggc tgaagtcctt gaagaacttc tcgtagccgt cctgcagcac
     1681 ggcccggagg aactggtcct tggtcttgaa atggtaatgg aacatgccgg gattgacccc
     1741 ggcccgggcg gccacctggc gcaggcccag gcggctgaag ctggaatccc gcaacagttc
     1801 ctgggccgcc tggatgaggc gctggtcggt gttcttcgag gggcgggcca tgccggcctc
     1861 cggatccgat aattagtcga ctgcctaata accaggataa cggggcgggt ggggaaagaa
     1921 aagggttatt ccaggcgcag gcgggggcgt tttttggggg cggg
//
LOCUS       DASTJJ010000291         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1632527, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000291 DASTJJ010000000
VERSION     DASTJJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632527"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /gene="glyS"
                     /locus_tag="VFR02_03700"
     CDS             complement(<1..1621)
                     /gene="glyS"
                     /locus_tag="VFR02_03700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET9869588.1"
                     /translation="MILKDAVLEIGCEELPSSYIRPAVDQMTAAVQAALKDHRLSPQQ
                     VTAYYTPRRLALFLKGLPEMQQETVKEVTGPLVSVAFDAHGLPTRAGEGFAKAQGVSA
                     ASLKRAATPKGEVVVAIKKTGGRLTSEILSEVFPEVIKKVRFPKVQRWGNGDFLFARP
                     IRWVCAVFSRSVVPFEVAGLFSSRETYGLRAKKLGPLDVIEAGDYLEVLRQGGVIADP
                     KERRQLAWDKIQEAAKALGGQDKVQVVEDPALLDDVANLVEAPEAILGSFDPHYLGLP
                     DQVIVTAMREHQKYFSLRDSGKVLLPYFIGVINGAPKDKQVVIRANEGVLKARLEDAK
                     FFVAEDRKVPMETWAEKLSGITWLEGLGTMADKSQRLSKLVKKVGELCGVPKEKTAVA
                     EEAALLCKADLATNIIREKEFNSLQGYMGGFYVLSAKEPVRYDKQAVGEAIQRHYWPR
                     FSGDQLPGTHESAVLSIADKMDSIAGCFKAGLIPSGSQDPLALRRQALGILQIILERK
                     YPVPLDSLVAAAQKQYGGKAKAGAAKDVVEFL"
     gene            complement(1618..>2450)
                     /locus_tag="VFR02_03705"
     CDS             complement(1618..>2450)
                     /locus_tag="VFR02_03705"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET9869589.1"
                     /translation="GCVIQQPYDLEKGAGTFNPATFFRVLGPEPYQVAYVDPCRRPKD
                     GRYGENPNRMQHYYQYQIILKPSPDNMVELYLESLERIGIRQDEHDIRFVEDDWESPT
                     LGASGLGWQVWMDGIEITQFTFFQTTGGLELKPVSGEITFGLERIAAVVQGVDSVWDI
                     RYGGKDITYRDIFHPSEKAFSAFNFEHANTKMLLRHFKDYEGEVRRLLELDLVEPAYD
                     YVVKASHTFNLLDARGAISVAERTHFIGRVRGMAKAVATAYVKEREGLGFPLLRKAQA
                     "
BASE COUNT          397 a          798 c          799 g          456 t
ORIGIN      
        1 tgaggaactc caccacgtcc ttggccgcgc cggccttggc cttgccgccg tattgttttt
       61 gggcggccgc caccagcgag tccagcggga ccgggtactt gcgttccagg atgatctgca
      121 ggatgcccag ggcttggcgg cgcagggcca aggggtcctg ggaacccgag gggatgagcc
      181 cggccttgaa gcagcccgcg atcgagtcca ttttatcggc gatgctcaga acggccgatt
      241 catgggtccc gggcaattgg tcgcccgaaa agcggggcca ataatgccgt tggatggcct
      301 cccccaccgc ctgcttgtcg tagcggacgg gctccttcgc ggacaggacg tagaacccgc
      361 ccatgtagcc ctgcaggctg ttgaactctt tttcccggat gatgttggtg gccaggtcgg
      421 ccttgcacag gagcgccgcc tcttccgcca cggcggtctt ttccttcggg acgccgcaaa
      481 gttccccgac cttcttgacc aacttggaaa gccgctggga cttgtccgcc atggtgccca
      541 gcccttccag ccaggtgatg ccggacagtt tttccgccca ggtctccatg ggcaccttcc
      601 ggtcctcggc cacgaagaac ttggcgtcct ccaggcgggc cttcaacacg ccctcattgg
      661 cccggatgac cacctgcttg tccttgggcg ccccgttgat gaccccgatg aaatagggca
      721 acaggacctt ccccgagtcc cgcagggaga aatacttctg gtgttcccgc atggcggtca
      781 cgatgacctg gtccggcagg ccgaggtaat gggggtcaaa ggagcccagg atggcctccg
      841 gggcctccac caagttggcc acgtcgtcca gcaaggcggg gtcctccacg acctgcacct
      901 tatcctggcc gcccagggcc ttggccgcct cctggatctt gtcccaggcc aattggcgcc
      961 tctctttcgg gtcggcgatg acgccgccct ggcgcagcac ttccaggtag tcgcccgcct
     1021 cgatcacgtc cagggggccc agtttcttgg cgcgcaggcc gtaggtttcc cggctgctga
     1081 aaagcccggc cacctcgaag gggaccaccg agcgggaaaa gaccgcgcag acccagcgga
     1141 tggggcgcgc gaagaggaaa tcgccgttgc cccagcgctg caccttgggg aaacggacct
     1201 tcttgatgac ctcggggaat acctcggaca ggatctcgga ggtcaaccgc cccccggtct
     1261 tcttgatggc caccaccact tcccccttgg gggtggcggc gcgcttgagc gaggccgccg
     1321 agacgccctg ggccttggcg aagccttccc cggcccgggt gggcaggccg tgggcgtcga
     1381 aggccaccga caccaggggg ccggtgactt ccttgaccgt ttcctgctgc atctcgggca
     1441 ggcccttgag gaacagcgcc aggcgccggg gcgtgtaata ggcggtcacc tgctgggggg
     1501 agagccggtg gtccttcagg gcggcctgga ccgccgccgt catctggtcg acggccgggc
     1561 ggatatagga ggaaggcagt tcctcgcagc cgatctcgag cacggcgtcc ttgaggatca
     1621 tgcctgggcc ttcctcagca aggggaagcc caacccctcg cgttccttca cgtaggccgt
     1681 ggccacggcc ttggccatgc cccgcacccg gccgatgaag tgggtgcgtt cggccactga
     1741 aatggccccc cgggcgtcca gcaggttgaa ggtatgggag gccttgacca cgtagtcata
     1801 ggcgggttcc accaggtcca gttccagcag gcgccggacc tcgccctcgt aatccttgaa
     1861 gtgcctgagc agcatcttgg tgttggcgtg ctcgaagttg aaggccgaaa aggccttttc
     1921 ggaggggtgg aagatgtccc ggtaggtgat gtccttgccg ccgtaacgga tgtcccatac
     1981 gctgtccacg ccctgcacca cggccgcgat gcgctccaag ccgaaggtga tctcgcccga
     2041 cacgggcttc agctccaggc cgcccgtggt ctggaagaag gtgaactggg tgatctcgat
     2101 gccgtccatc cagacctgcc agcccaggcc cgaggccccc agcgtgggcg attcccaatc
     2161 gtcctccacg aaacggatgt cgtgctcgtc ctggcggatc ccgatccgtt ccaggctttc
     2221 caggtaaagt tccaccatgt tgtccggcga gggcttgagg atgatctggt actggtagta
     2281 atgctgcatg cggttggggt tctcgccgta gcgcccgtcc ttggggcggc ggcaggggtc
     2341 cacgtaggcc acctggtagg gttcggggcc cagcacgcgg aaaaaggtgg ccggattgaa
     2401 ggtgcccgcg cccttttcca ggtcgtaggg ctgctggatg acacaaccct
//
LOCUS       DASTJJ010000292         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_531462, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000292 DASTJJ010000000
VERSION     DASTJJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531462"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFR02_03710"
     CDS             <1..997
                     /locus_tag="VFR02_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9869590.1"
                     /translation="VKCLAGYLLCGVSFLVFTSRYAQGHLPVSAIVWTMYLTAFAGGI
                     IRAFSSPTTFTLLSLVVPRNLYANATTWSSASWQSGAVLGPLAGGFLYAWSGATGVFA
                     TVILFHLMGLVSLVYIAPKPVFYEKKDQPALQRLSEGVRFVFRTKEILAALSLDMFAV
                     LFGGAVALLPIFADDILKVGAEGLGALRAAPAIGSCVTLLLLAYVPLEKKAGVKLLGA
                     VFCFGLSIICFGLSGLFWLSWTALFLSGVFDGVSVVIRNTILQLKTPDDMRGRVSAVH
                     TMFVGSSNEFGSFESGVTAKWMGTVPAVIFGGCMTLAVVTVTWLVSPRLRRLELR"
BASE COUNT          172 a          376 c          349 g          249 t
ORIGIN      
        1 ggtcaaatgc ctggcgggat acctgctgtg cggcgtctcc ttcctggtgt tcaccagccg
       61 ttatgcccag ggacatctcc cggtcagtgc catcgtctgg accatgtacc tgacggcctt
      121 tgccgggggc atcatccggg ctttctccag ccccaccacc tttaccctgc tctccctggt
      181 ggtgccccgc aacctgtacg ccaacgccac cacctggagc agcgcctcct ggcagtcggg
      241 cgcggtgctc ggaccgctgg cgggaggctt cctctatgcc tggagcggcg ccacgggcgt
      301 cttcgctacg gtgatcctct ttcacctgat gggtctggtc tccctggtgt atatcgcccc
      361 caagccggtc ttctacgaga aaaaggacca gccggccctg cagcgcctga gcgagggcgt
      421 gcgcttcgtc tttcggacca aggaaatcct ggccgccctg tcgctggata tgttcgccgt
      481 gttgttcggc ggggcggtgg cgctgcttcc catcttcgcc gacgacatcc tgaaggtagg
      541 cgccgaggga ctgggtgccc tgagggccgc cccggccatc gggtcctgcg tgacgctgct
      601 gctgctggca tacgtgcccc tggaaaagaa agccggcgtc aagctcctgg gggcggtatt
      661 ctgcttcggg ctgtccatca tctgcttcgg gctgtcggga ctgttctggc tttcctggac
      721 ggcgcttttt ctctccggtg ttttcgacgg ggtgagcgtg gtcatccgca ataccatcct
      781 gcagctcaag acgccggacg acatgcgggg ccgcgtctcg gcggtgcaca ccatgttcgt
      841 cgggtcgtcc aacgagttcg gctccttcga aagcggcgtc accgccaaat ggatgggcac
      901 cgttccggct gtcatcttcg ggggatgcat gaccctcgcc gtggtaaccg tcacctggct
      961 ggtctccccc aggctgcgca ggctggagct acgctagaca cctcgctcac ggccggcgta
     1021 tctcggagga ttttcgtaac ttgaagcgta ccgacccggg gtatcttccc cgggggagcc
     1081 tttacaccct ttaaactccg gatcatgaag gcatacgacg aaaagcacat caagaacatc
     1141 gccctt
//
LOCUS       DASTJJ010000293         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_531609, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000293 DASTJJ010000000
VERSION     DASTJJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531609"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..662)
                     /locus_tag="VFR02_03715"
     CDS             complement(144..662)
                     /locus_tag="VFR02_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9869591.1"
                     /translation="MRPQLPQLVPSEVCLECPVCCRFPEKQASLSPFFFPEERAQAEA
                     RGEGPGAFRRPGASKAELVECGHGFACTFFDPGDHTCRIYPVRPLDCSMYPAAVMWSQ
                     DRRQVLMGVDTKCPALGRPDVAARLDHYLDWIQRYLESADVSPSLLRHPEWITDFQED
                     VVESRPLSLGKA"
     gene            complement(730..912)
                     /locus_tag="VFR02_03720"
     CDS             complement(730..912)
                     /locus_tag="VFR02_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869592.1"
                     /translation="MPNEKQVVEPKLKSVGKITLGVGVGVLVVGYMFLATGSMSLAPV
                     LIIGGLITVGLGIYNL"
     gene            complement(1042..>1650)
                     /gene="serS"
                     /locus_tag="VFR02_03725"
     CDS             complement(1042..>1650)
                     /gene="serS"
                     /locus_tag="VFR02_03725"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9869593.1"
                     /translation="NLHRGETLPAGSLPKKYAAFTPCYRREAGSYGKDTKGLIRLHQF
                     HKVELFQFVEPEKSMEALESLTADAEDILKKLGLPYRVVALATGDKSFASTKTYDLEV
                     WLPSQGAYREISSCSNCTDFQARRGGIKFKREGGGKPELVHTLNGSGLAVGRTWLAIL
                     ENYQRADGSVTVPEVLRPYLGGLDVLTKHDPFTYTNETGLET"
BASE COUNT          327 a          510 c          519 g          294 t
ORIGIN      
        1 ccgggatttc cagccaccag ggcctgagga aaaagaaggc caacagggcc cccgccacga
       61 acgcggcggt gatgccccac caataacgaa cccaacgcat ggcccggggg ggccgtaaag
      121 cctgcaacgt cgctaccata agatcaagcc tttcccagcg acagcgggcg cgactccacg
      181 acgtcttcct gaaaatcggt gatccattcg ggatgccgca ggagggacgg ggacacgtcg
      241 gcgctttcca agtatcgctg tatccaatcg agatagtggt ccaaccgggc ggccacgtcg
      301 ggccgcccca gggcggggca tttggtgtcc acgcccatca acacttgacg gcggtcctgg
      361 ctccacatca ccgccgcggg gtacatcgaa caatccaggg gacggaccgg atagatccgg
      421 cacgtatggt ccccggggtc aaaaaacgta caagcgaacc catgcccgca ctccaccagt
      481 tcggctttgg aggcgccggg acggcgaaaa gcccccggcc cctcgccccg ggcttcggcc
      541 tgggcccgtt cctcgggaaa gaagaagggc gacagggagg cctgtttttc ggggaaccgg
      601 cagcagaccg gacactccag gcagacctcg ctcgggacca attggggcaa ttgcggacgc
      661 atggcttctt tataaaatag caaagtcgcg ggggatcggg ggggttattt gccgggaaag
      721 tgggggaggc tagaggttgt agatgcccag cccgacggtg atgaggccgc cgatgatgag
      781 caccggggcc aggctcatgg agcccgtggc caggaacata tagcccacca ccaggacgcc
      841 cacccccacg ccgagggtga tcttgccgac ggacttcagt ttgggttcaa ccacctgttt
      901 ttcgttgggc atgggttcct cgctgggtca ttttatcaaa atcggggaaa aaccccagga
      961 atatcggctt tttatcacat ccaaaactgg atattcacca ccaaggcacc aagttcacca
     1021 aggaaagcga agcattcgat cctaggtctc caagccagtt tcatttgtat aggtgaaagg
     1081 atcatgtttg gtcaggacgt ccaagccgcc tagatagggg cgcaagactt cgggcacggt
     1141 cacgctacca tcggcccgtt ggtagttctc caggatggcc agccaggtgc ggcccacggc
     1201 caggcccgag ccgttgaggg tatggaccag ttcgggcttg ccgcccccct cccgcttgaa
     1261 cttgatgccg ccccgccggg cctggaagtc ggtgcagttg ctgcagctgg agatctcccg
     1321 gtaggccccc tggctgggca gccagacctc caggtcgtag gtcttggtgg aggcgaagct
     1381 cttgtccccc gtggccaacg ccaccacccg gtagggcagg cccagtttct tcaggatgtc
     1441 ctcggcgtcc gccgtgagcg actccagggc ctccatgctc ttttcgggct ccacgaactg
     1501 gaacagctcc accttgtgga actggtgcag gcggatgagg cccttggtgt ccttgccgta
     1561 gctgcccgcc tcccggcggt agcagggcgt gaaggccgcg tattttttcg gcagggatcc
     1621 cgccggcagg gtctcgccgc ggtgcaggtt
//
LOCUS       DASTJJ010000294         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_653825, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000294 DASTJJ010000000
VERSION     DASTJJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653825"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFR02_03730"
     CDS             complement(<1..209)
                     /locus_tag="VFR02_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9869594.1"
                     /translation="MTPIEPTQAAGWLAGAQQFVSAAKLHPILVNFTAALVPFSVAAD
                     AAARWRKDETLRHAAWWALLAAALIT"
     gene            complement(336..497)
                     /locus_tag="VFR02_03735"
     CDS             complement(336..497)
                     /locus_tag="VFR02_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET9869595.1"
                     /translation="MLLLLVLVLACLWAVSGGYAKGGGRSALDILKERYARGEITKQE
                     FEDKKRDIA"
     gene            complement(924..1193)
                     /locus_tag="VFR02_03740"
     CDS             complement(924..1193)
                     /locus_tag="VFR02_03740"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9869596.1"
                     /translation="MKRILSWTVLGVWMSVGQAVLADNSMGNMTGMSTPAMSATPAMK
                     HKIKMKRTKKKTAKVAKVYACPMDGYTSNKPGKCPQCGMDLMEKQ"
     gene            complement(1255..>2312)
                     /locus_tag="VFR02_03745"
     CDS             complement(1255..>2312)
                     /locus_tag="VFR02_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9869597.1"
                     /translation="PGTRSAFAERVQGGYFVDITPRRDQLARFGMKLEDLNVIIESAI
                     GGDDVTETVEGRARYPVAVRLESEFRDNVEKLQRLPVMTPIGTRVQLAQLADITVTHG
                     PDMIRDENGFLAGYVYVDVTGRDIGGYVKAAKKAVGEQVQLPPGVSLLWSGQYEFMLA
                     AAQRLRIMVPLALVLVFILIYFNTRSITQTLMILLAIPFSLVGAVWLLWALGYNLSVA
                     VWVGMIALAGLDAETGVIMMLYLNLAYQKRRTEGRLKGLADLKEAVLEGAVQRVRPKL
                     MTVGTAWIGLVPILFSTGIGSDVMKRIAAPMVGGVLTSLVLVLLVYPAIFMLWKWNTE
                     LKPGEPKGFWKWVGKIG"
BASE COUNT          474 a          778 c          565 g          495 t
ORIGIN      
        1 gtgatgagcg cggcggccag gagggcccac cacgccgcgt ggcgcagggt ctcatccttc
       61 cgccaccggg cggccgcgtc cgcggcgacc gaaaaaggga ccaacgccgc cgtgaagttg
      121 accaggatgg ggtgcagctt cgccgccgaa acgaattgtt gcgcccccgc cagccatccg
      181 gccgcctgcg tcggttcaat gggtgtcatg ccatcccttt ctttaaaacg atggatgccc
      241 ttcttcagaa caccctagaa ccccattcat cccatttgca agcagggatc ggaaagtccc
      301 cccttccggc ccccttctgc gtgccggtct tgggctcaag caatgtcgcg ttttttatcc
      361 tcgaattcct gcttggtgat ctcgccgcgg gcataccttt ccttcaggat gtccaaggcc
      421 gaccgtcccc cgcctttggc atatccgccc gagacggccc aaagacaggc caggacgagg
      481 accaatagca gcagcatccc ccagcccatg ccgaaccctc cgactccaaa tccatatccc
      541 atggtctcca cccccttccg gccttcaagt ccagtctaaa gccgccgggc aggggccgtg
      601 atgacaccgg tcatagaccc ggcctacccg atcttccgga cgatccccca gaaccatccc
      661 cgaagctcca gcctatcccg aactgggaca tatccttgtt tcgagtccct ttcccaacgt
      721 tgccacatca atattctgaa acttattgcc cggcacatgg atggaaccgc gttgtttgcc
      781 acaagccacc tcttcgacaa gcatcaggac aaaacttagt ttttcgccat ttaccctctg
      841 gatttcatcc ccggggggac aaaaagccta tttaagatgg acccttcatt ccacccacca
      901 tccccgaaat gacgaatgct gttctattgt ttttccatta gatccatccc gcactgcggg
      961 catttccccg gtttgttgct ggtataacca tccatcggac aggcatagac cttggccacc
     1021 ttggctgttt ttttctttgt ccgcttcatc ttgatcttat gtttcattgc cggcgtggca
     1081 ctcatcgccg gcgttgacat tccggtcata ttgcccatgg aattgtccgc taaaacggcc
     1141 tgaccgacag acatccaaac tcccaatacc gtccaactta aaatcctttt catgacttgg
     1201 ctccttttat ttagaattat catcctcggc ccacgttgga gtggaaagcc gcccttaccc
     1261 tatcttccct acccatttcc aaaatccctt cggttctccc ggctttaatt cagtgttcca
     1321 tttccacagc atgaaaatgg cgggatagac caacagcacc agcaccagcg acgtcaggac
     1381 ccctcccacc atgggcgcgg cgatgcgttt catcacgtca cttccaatgc ccgtggaaaa
     1441 gaggatcggc accaaaccaa tccaggcggt gccgaccgtc atgagcttgg gccggacccg
     1501 ctgtaccgcg ccttccaaga ccgcctcctt caagtcggcc aggcccttca aacggccctc
     1561 cgttcttcgt ttttggtaag ccaggttcag gtacagcatc atgatgacac ccgtctccgc
     1621 gtccaacccc gccagggcga tcattcctac ccagaccgcc accgacaggt tgtagcccaa
     1681 ggcccacaag agccacaccg ccccaacgag ggaaaagggg atggccaaga ggatcatgag
     1741 ggtttgggtg atcgatctcg tgttgaagta gatcaaaatg aaaaccaaca ccagggccag
     1801 aggcaccatg atccggagcc tttgggcggc cgccagcatg aattcatatt ggccgctcca
     1861 aagaaggctc acccccggcg gaagctggac ctgttcgccc accgcctttt tggccgcctt
     1921 cacgtagccg ccgatgtccc ggcccgtgac gtccacgtag acgtacccgg ccaggaagcc
     1981 attctcgtcc cggatcatgt ccggtccgtg agtgaccgtg atgtcggcca attgggccag
     2041 ttgcacccgg gtcccgatcg gggtcatcac cggcaagcgt tggagttttt caacgttgtc
     2101 ccggaattcc gactccaagc ggaccgccac cgggtagcgg gcccggccct ccaccgtctc
     2161 ggtcacgtcg tcgccgccaa tggccgattc gatgatcaca ttcaggtctt ccaatttcat
     2221 cccaaagcgg gccaattggt cccggcgcgg ggtgatgtcc acgaaataac cgccctggac
     2281 ccgttccgcg aaggcgcttc gggtgccggg ga
//
LOCUS       DASTJJ010000295         1387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1265096, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000295 DASTJJ010000000
VERSION     DASTJJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265096"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFR02_03750"
     CDS             <1..927
                     /locus_tag="VFR02_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869598.1"
                     /translation="RFDDSAALQSNEDPVKQHLEDLGLVAVQYLSYGGGVLYDHALDT
                     DESKGAYQFFADRQSGAVTDIANAGHIYDLSETNTVEDVETLIVSYSTPLPLSAFQFL
                     SAGFSLKYHYGSAYSRTFLDGSFIAGTGGTPTYTRTTSTSGLGLSTDMGFLAKLTDSL
                     QLGVLMQNLTSAFNWTAQKQAYSLDSNGNESPSGPAQSVTISAPLPYAVKIGVLAEPP
                     GSNILLNAQVDFTQAKTHWRVGVERFYPANNLVVRLGTFNDEVSGDQIWTFGAGFETT
                     SFSLDGAFLTRSLPNLQDSIALGGALDAAIRF"
     gene            complement(1089..>1387)
                     /locus_tag="VFR02_03755"
     CDS             complement(1089..>1387)
                     /locus_tag="VFR02_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9869599.1"
                     /translation="MGALAGVAGVVLTARVGAATSDAGHMMELDAIAAAVIGGTSLMG
                     GRGSVWGALLGALVMASLDNGMGLMNTEPFWQPILKGAILIAAVGADMAGRRKG"
BASE COUNT          261 a          491 c          387 g          248 t
ORIGIN      
        1 cggttcgacg attcggccgc cctccaatcg aatgaggacc ccgtcaaaca gcacctggag
       61 gacctggggc tggtcgccgt ccaatacctt tcctacggcg gcggggtgct ctacgaccac
      121 gcgctggaca ccgacgaatc caagggcgct tatcagttct tcgccgaccg gcagtccggg
      181 gcggtcacgg acatcgccaa cgcggggcat atttacgacc tcagcgaaac gaacaccgtt
      241 gaggacgtgg agaccctgat cgtgagctac tccacccccc tgcccctgtc ggccttccaa
      301 ttcctttcgg ccgggttcag cctgaaatac cattatggga gcgcttacag ccggaccttc
      361 ctggacggtt ccttcatcgc cggcacgggc ggcaccccca cctacacccg gaccacttcc
      421 acctcgggcc tgggcctgag caccgacatg ggattcctgg ccaagctcac cgactcattg
      481 cagttggggg tcttgatgca gaacctgacc tcggccttca actggaccgc ccaaaagcag
      541 gcctacagtc tggactcgaa cggcaacgag agcccttcgg ggcccgcgca aagcgtgacc
      601 atctccgctc ccctgcccta tgccgtcaag atcggggtgc tggccgaacc gccggggtcg
      661 aacatcctat tgaacgccca ggtggacttc acccaggcca agacccattg gcgggtcgga
      721 gtggagcgtt tttaccccgc caacaacctg gtggtgcggc tggggacctt caatgacgag
      781 gtctcggggg accagatctg gaccttcggg gcgggattcg agaccacttc cttcagcctg
      841 gacggggcct tcctgacccg cagcctgccc aacctgcagg attccatcgc cctgggcggc
      901 gccctggacg cggcgatccg gttctagaag tcatttcaaa acctctttta ccgcagaggc
      961 gcaaagtgaa gacaagaggc gaaaagccgg ggaaataaac attatttttc ctgcgtctcc
     1021 acgtctctgc ggttaaaaat tttcgctttt agggttttga aacaggttct aggacctcac
     1081 cggccggcct aacccttccg ccgtcccgcc atgtcggcgc ctaccgccgc gatgaggatg
     1141 gcccccttca ggatcggctg ccagaagggt tcggtgttca tcaatcccat gccgttgtcc
     1201 agcgacgcca tgaccagcgc ccccaacaac gccccccaca cgctcccgcg cccccccatc
     1261 aaggacgtgc cgccgatgac cgccgccgcg atggcgtcca gttccatcat gtgcccggcg
     1321 tccgaggtgg cggcccccac ccgggccgtc agcaccacgc ccgccacccc ggcgagcgcg
     1381 cccatca
//
LOCUS       DASTJJ010000296         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_287761, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000296 DASTJJ010000000
VERSION     DASTJJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287761"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFR02_03760"
     CDS             <1..251
                     /locus_tag="VFR02_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869600.1"
                     /translation="VAIYGKFYNLDNSDYALLVHVMDTVQKGGHDRDILLDARMSPTP
                     TVTPTVTPTPGPTLWKKLQNAMNPKEEPTGTVTPNAGP"
     gene            256..1023
                     /locus_tag="VFR02_03765"
     CDS             256..1023
                     /locus_tag="VFR02_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9869601.1"
                     /translation="MAAVHSRILGDGPRRLVLLHAFPLSSAMWDGLRPPSGHHLVLPD
                     FPGFGTSPATSSPLSFTDLSQALQDHLTALPKAEKTLLGGISMGGYWALEYARLHQAH
                     LEGLLLISTRAGVDKPEGRQNRLKMAERAENEGMEGLVEALLPGLLGKSTFEKKPLVV
                     EKLRQWVKLAPPQAVALAQRTMAGRRDQTDLLPSIALRTLVLAGQEDALIPPTEAQSM
                     AAAIPGAGLRVLEGVGHLIPLETPGSFQAILNEFESL"
BASE COUNT          272 a          410 c          364 g          221 t
ORIGIN      
        1 gggtggcgat ctacggcaag ttctacaatc tggacaactc ggactacgcc ctcttggtgc
       61 acgtgatgga cacggtccaa aagggcggcc atgaccggga tatcctgctg gacgcccgga
      121 tgtcgcccac gcccaccgtg acccccacgg tcacacccac ccccgggccc acgctgtgga
      181 agaagctgca aaacgcgatg aatcccaagg aagaacccac cgggaccgtc actcccaacg
      241 cggggcccta gcggcatggc ggcggtccac tcccggatcc tgggggatgg cccccgccgc
      301 ctggtcctgc tccacgcctt tcccctgtcc agcgccatgt gggacggcct aaggccgccc
      361 tccgggcacc acctggtact gcccgacttt cccggcttcg ggacctctcc cgccacgtcg
      421 agccccttga gctttaccga cctatcccaa gccctccagg accacttgac ggccctcccc
      481 aaggccgaaa agacccttct tgggggcatc tccatgggtg gctattgggc cctggagtac
      541 gccaggctcc accaggccca cttggagggt ctgctgctca tttccacccg ggccggggtg
      601 gataagcccg agggccgcca gaaccggctg aaaatggccg aacgggccga aaacgagggc
      661 atggagggcc tggtcgaggc cctcctgcct ggcctgctgg ggaagagcac cttcgaaaaa
      721 aagccccttg tggtggaaaa actgcgccaa tgggtgaagc tggccccgcc ccaggccgtc
      781 gcccttgccc aaaggaccat ggccgggcgc cgggaccaga ccgatctctt gccctcgatc
      841 gccctgagga ccctggtgct ggccggccag gaagacgcct tgatcccgcc cacggaagcc
      901 cagtccatgg ccgccgccat cccgggcgcg gggttgcgcg tcctggaggg agtcggtcat
      961 ttgatcccgc tcgaaacacc cggaagcttt caagcgatac tgaatgagtt cgaaagcctt
     1021 taaaattgcg gagttcttca aaagggcgag cagaggtata gaaccatgcg cgtgaaatgc
     1081 atttgtcgaa tatgtggcag acattacgaa tatatctacg agaacaaagt atcaattggc
     1141 aatcacaaca agaaagtttg caattcttgt aaaactaacc accgccgttt tgcgctcaaa
     1201 gaccgaatga tagcttataa aggcggtagc tgtaatgtat gtggttacaa gaagtgcaac
     1261 cgcgcct
//
LOCUS       DASTJJ010000297         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1633202, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000297 DASTJJ010000000
VERSION     DASTJJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633202"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1120)
                     /gene="tgt"
                     /locus_tag="VFR02_03770"
     CDS             complement(<1..>1120)
                     /gene="tgt"
                     /locus_tag="VFR02_03770"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9869602.1"
                     /translation="STGREAGSRARAGRLSTDHGTMETPVFMPVGTAGAVKAVTFGQL
                     QADVRAPIILANTYHLYLRPGTAILEKAGGLHRFSGWNRSILTDSGGYQVFSLAAKRK
                     ITEEGVLFQSHIDGSRHMFSPEKVMDIQRTIGADIIMAFDECPPWPSEYRYAVDSMQL
                     THRWLDRCISRLRETGPLYGHSQALFPIVQGSTFTDLRRRSAEEVAARDCPGNAIGGL
                     SVGEPEEMMYGMCSAVCELLPAAKPRYLMGVGTPWNILECIALGVDMFDCVMPTRNGR
                     NGMLFTAEGVIHIRNLKWAEDFSPVDPATPCFASREYSRAYLRHLFVAGEILGMQLAS
                     LHNLCFYLNLVKQAREHILQGDFLQWKEGMVKNMRVRL"
BASE COUNT          198 a          356 c          339 g          227 t
ORIGIN      
        1 tataaccgca cccgcatatt tttcaccatt ccttctttcc actgcaggaa atcaccctgc
       61 aaaatatgct cccgcgcctg ttttaccaaa ttaaggtaaa aacaaaggtt gtgcaggctc
      121 gccagctgca tccccaggat ctcccctgcc acgaagaggt ggcggaggta ggcgcgggaa
      181 tattcccggc tggcgaaaca gggggtggcg ggatccacgg ggctgaaatc ctccgcccat
      241 ttcaagttcc tgatgtggat caccccttcg gcggtgaaaa gcatgccgtt gcgcccgttg
      301 cgggtgggca tcacgcagtc gaacatgtcg acccccaggg cgatgcactc caggatgttc
      361 cagggcgttc ccacccccat gaggtaacgg ggtttggcgg cgggcagcag ctcgcatacc
      421 gcgctgcaca tgccgtacat catctcctcg ggttctccca cactcagccc ccctatggca
      481 ttgccggggc agtccctggc ggccacttct tccgctgacc ggcgccgcag gtccgtgaat
      541 gtgcttccct gcacgatggg gaacagcgcc tggctgtggc cgtaaagggg cccggtctcc
      601 cgcagccgcg agatgcaccg gtccagccag cggtgggtca gctgcatgga atccacggca
      661 taccggtact cgctgggcca gggagggcat tcgtcgaagg ccatgatgat gtcggctccg
      721 atggttcgct ggatgtccat gaccttttcc gggctgaaca tatgcctgga tccgtcgata
      781 tgggactgga acagcacgcc ttcctcggtg atcttgcgtt tggcggcgag ggaaaacacc
      841 tggtagccgc cgctgtcggt caggatggaa cggttccagc cactgaagcg gtgcaggccg
      901 ccggctttct ccaggatagc ggtacccgga cgaaggtaca ggtggtaggt gttggccagg
      961 atgatggggg cccgcacatc cgcctgcagc tgcccgaagg taacggcctt tacggcgccg
     1021 gcggtgccca ccggcatgaa caccggcgtt tccattgtgc catggtccgt gctcagcctc
     1081 cctgcccttg ccctgctccc ggcttccctt cccgtacttt
//
LOCUS       DASTJJ010000298         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1388557, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000298 DASTJJ010000000
VERSION     DASTJJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388557"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFR02_03775"
     CDS             <1..319
                     /locus_tag="VFR02_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869603.1"
                     /translation="LDLQDWAFLGGGHAQYHFRFLAEAKVTFPGEAWQPYLLAGPGLS
                     LRTFYYSGDSAADLDLLAGAGAQVSLGGDSHLFVQAALNALPGASPAVLEFPLTAGLW
                     VGL"
     gene            631..2565
                     /locus_tag="VFR02_03780"
     CDS             631..2565
                     /locus_tag="VFR02_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019613669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9869604.1"
                     /translation="MSVTTELKHLSEKEKESLEVAEGARETEWTKPSFVAELFLGRLR
                     LDLINPYPLQSPEDKAVGDEFMTRLEAFLRDNLDPDEVDRTGILPQGVIDGLAKLGCW
                     GMKIPKEYGGLGLSQINYNRAVAMVASYCGSTAVWLSAHQSIGVPQPLKLFGNEAQKK
                     KYFPMFAKGAVSAFALTEPGVGSDPARMTVTAEPSADGKSWTINGTKLWCTNGLVADV
                     MVVMAKTPSKMVKGREKRQITAFIVEKGTPGIEYVHRCDFMGIRAIQNGVIKFNNVKV
                     PSENILWAEGQGLKLALITLNTGRLTLPAASAGMSKQALQIAKIWGKEREQWGAPIGK
                     HEPGASKIANMAASTFAQEAVTYYTSALVDRGGADIRLEAAMAKLFCSVASWKIVDDL
                     MQLRGGRGYETARSLRGRGEKGYAVERMMRDARINRIIEGTDEIQHLFIAREAMDKHM
                     SLAGPLLAPSSPLGEKFKALVHCALFYPFWYPRLWLPTGFWPRYSAYGSLAGHMRYVE
                     RTTRKLARTLFHQMVKFGPKLERRQLILGRLVEIGTELFAMTAACAKVVTVEKQAPGE
                     TGPRELADLFCRQAKLRIHAYFDGVACNADTAESKVAAKVLDRRFEWVEKGIMVDDGE
                     GFKGKFGPPNTSEERRRYET"
     gene            2565..>2869
                     /locus_tag="VFR02_03785"
     CDS             2565..>2869
                     /locus_tag="VFR02_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869605.1"
                     /translation="MRRALKAALWGAGGLSALVAGYLLLLCFPGPLFAYHAQVGALTL
                     YDDDPIAPGTAQQVLLDVQRRLDRCPLDAGRPAQSVFLCDREWQHRLLMLSVPKAGGV
                     "
BASE COUNT          506 a          960 c          947 g          456 t
ORIGIN      
        1 actggacctc caggactggg ccttcctggg cgggggccac gcccaatacc atttccgttt
       61 cctggccgag gccaaagtca ccttccccgg ggaggcctgg cagccctacc tgctggcggg
      121 cccgggcctg tcgctgcgca ccttctatta cagcggggac agcgcggcgg acctggacct
      181 cttggccggg gcgggggcgc aggtatccct cggcggcgac agccatcttt ttgtccaggc
      241 ggccttgaac gccctgccgg gcgcctcccc cgcggtgttg gagttccccc tgaccgccgg
      301 gctttgggtg ggcctgtaag cccttcttcc cgccggggcg ggggagtaaa atagcgggga
      361 gctgtgcgcc ggcagggcac atgttttatt gtgtcattgc gaggaacgga atttgtgacg
      421 aagcaatctc ggtggatggg gttgtccgcg cccgggtctg acggcgtgtg aaaggcccca
      481 aaccgggacc gcttcgccgc taaaaccggt gaatggcgct cctcgcggtg accaagatcc
      541 gtttagccga acggggatct gcgaagcact cagccccaaa ctagaagccg cagggtcgct
      601 cccattccat ccgtaagaca aggacccgtc atgagcgtca cgaccgaact caaacacctg
      661 agcgaaaaag agaaggaatc cctggaagtg gccgaagggg cccgggagac cgaatggacg
      721 aagcccagct tcgtggccga gctgttcctg ggccgcctgc ggctcgacct catcaacccc
      781 tacccgctgc aatcgccgga ggacaaggcg gtgggcgacg agttcatgac gcgcctggag
      841 gccttcctca gggacaacct ggacccggac gaggtggacc gcaccggcat cctgccccag
      901 ggcgtcatcg acggcctggc caagctgggc tgctggggca tgaagatccc caaggaatac
      961 ggcgggctgg gcctgtccca gatcaactac aaccgggccg tggccatggt ggcctcctat
     1021 tgcggctcga cggccgtgtg gctttccgcc caccagtcca tcggcgtgcc ccagccgctc
     1081 aagcttttcg gcaacgaggc ccagaagaag aaatatttcc cgatgttcgc caaaggcgcg
     1141 gtctcggcct tcgccctcac ggagccgggc gtcgggtcgg acccggcccg catgacggtg
     1201 accgctgaac cctcggccga cggcaagagc tggaccatca acggcaccaa gctctggtgc
     1261 accaacggcc tggtggccga cgtgatggtg gtgatggcca agacgccctc caagatggtg
     1321 aagggccggg aaaagcgcca gatcacggcc ttcatcgtgg aaaagggcac gccgggcatc
     1381 gagtacgtgc accgctgcga cttcatgggc atccgggcca tccagaacgg cgtcatcaag
     1441 ttcaacaacg tgaaggtgcc ctccgagaac atcctctggg ccgagggcca gggcctgaag
     1501 ctggccctca tcaccctgaa caccggccgc ttgaccctgc cggcggcctc cgcgggcatg
     1561 agcaagcagg cgctgcagat cgccaagatc tggggcaagg aacgcgagca gtggggcgcg
     1621 cccatcggca agcacgagcc cggcgcctcc aagatcgcca acatggccgc ctccaccttc
     1681 gcccaggagg ccgtcaccta ctacacctcg gcgctggtgg accggggcgg ggccgacatc
     1741 cggctggaag ccgccatggc caagctcttc tgcagcgtgg cttcctggaa gatcgtggac
     1801 gacctgatgc agctcagggg cgggcggggc tatgagaccg ccaggtccct gcgcggccgg
     1861 ggcgagaagg gctacgccgt ggagcgcatg atgcgcgacg cccgcatcaa ccgcatcatc
     1921 gaggggaccg acgagatcca gcacctcttc atcgcccgcg aggccatgga caagcacatg
     1981 agcctggcgg ggcccttgct cgcccccagc tcgccactgg gcgagaagtt caaggcgctc
     2041 gtccactgcg ccctctttta ccccttctgg tatccccggc tttggctgcc caccgggttc
     2101 tggccgcgtt attcggccta tggctcgctg gcggggcaca tgcgctacgt ggaacgcacc
     2161 acgcgcaaat tggcccgcac gctcttccac caaatggtca agttcgggcc caagctggag
     2221 cgccgccagc tcatcctggg gcgcttggtc gagatcggca ccgagctttt cgccatgacg
     2281 gccgcctgcg ccaaggtggt gacggtggaa aaacaggcgc cgggggagac gggcccccgg
     2341 gaactggcgg accttttctg ccgccaggcg aagctgcgca tccacgccta tttcgacggc
     2401 gtggcctgca acgccgacac ggccgaatcg aaggtggccg ccaaggtgtt ggaccgccgg
     2461 ttcgaatggg tggaaaaggg gatcatggtg gacgacggcg agggcttcaa gggaaagttc
     2521 ggcccgccca acaccagtga agaacgccgc cgctacgaga cctgatgcgc cgggcgctga
     2581 aggccgcgct atggggggcc gggggccttt cggcgctcgt ggccggctac ctgctgctgc
     2641 tttgtttccc cgggcccctc ttcgcctacc atgcccaagt gggggccctc accctctatg
     2701 acgacgaccc gatcgcgccc gggacggccc aacaggtcct cttggatgtc cagcggcgcc
     2761 tggaccgctg ccccctggac gcgggccgtc cggcccagtc cgtcttccta tgcgaccggg
     2821 aatggcagca ccgactcctc atgctcagtg tccccaaggc ggggggcgt
//
LOCUS       DASTJJ010000299         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_899291, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000299 DASTJJ010000000
VERSION     DASTJJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899291"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFR02_03790"
     CDS             <1..562
                     /locus_tag="VFR02_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869606.1"
                     /translation="GPVSAEAPQDVLVCHMITCTNIVNNYPNDSVNYFYLNKNNQVCF
                     YLYFLLKPESRIHTVTVECYNPAGIRIAKYDQEFRTAFTDRLLTVGSDTYQWFLTTLT
                     LGMDHMRPEYGQTGLPKDTGLYTFNTSVDGRLVGITFFYVKNAPIQPPSSIPTVAPSA
                     PGASSLPSSFPMSTPVSSQAMPHGIP"
     gene            571..1605
                     /gene="holA"
                     /locus_tag="VFR02_03795"
     CDS             571..1605
                     /gene="holA"
                     /locus_tag="VFR02_03795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9869607.1"
                     /translation="MYFDEFIQKLSQPSWPSLVLLYGDSEGVMAEGLTRLREAFRKRA
                     PGGTVDVREGGEGDLAGLLAEAQTASLFADRRLLVLRGAGKALGGRSEAAQARLADYA
                     RDPNPDATVVFAAEGLRKTSKIVGWFEKNGWAAQCSDIPDWKLGPWVRQQARALGLEL
                     GDEGAQALLQKTGTDIAYLNRALEHLALFIHPARKPSLQDVLDLPIPGAEAEIFPFLD
                     DWGMRRTEKALLGLRRMAPGAENGLVFMLYQRVRDLLGVAAGRAEGLDQSQLAQRLGI
                     HPFRVKNLWEQSSQYTAGELRGALRDLVRIQAGLVTGRVGKSGLSTLVEWWIVKWGKN
                     PLAAEAANGG"
     gene            complement(1606..1869)
                     /gene="rpsT"
                     /locus_tag="VFR02_03800"
     CDS             complement(1606..1869)
                     /gene="rpsT"
                     /locus_tag="VFR02_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9869608.1"
                     /translation="MPIIKSSIKDVRRIKRRTARNSAEKNRIRTAIKAVRAAKTQEEA
                     AAKLRLAMKALDKAASNGVLKRQTASRNIARLSSFVHKQFKSK"
BASE COUNT          342 a          674 c          695 g          341 t
ORIGIN      
        1 cggcccggtc tccgccgagg ccccgcagga cgtgctggtc tgccacatga tcacctgcac
       61 caacatcgtg aacaactacc ccaacgacag cgtgaactac ttctacctca acaagaacaa
      121 ccaggtctgt ttctaccttt acttcctcct caagcccgaa agccgcatcc acaccgtcac
      181 ggtggaatgc tacaacccgg cgggcatccg catcgccaaa tacgaccagg aattccggac
      241 ggccttcacg gaccgcctct tgaccgtggg gagcgacacc taccaatggt tcctgaccac
      301 cctgaccctg ggcatggacc acatgcgccc cgaatacggg cagaccggcc tgcccaagga
      361 caccgggctc tataccttca acacctcggt ggacggacgc ctggtgggca tcaccttctt
      421 ctacgtgaag aacgcgccca tccaaccgcc ctcctccatc ccgaccgtgg cgccctcggc
      481 cccgggggcc tcgtccctgc cctcgtcctt ccccatgagc acgcccgttt ccagccaagc
      541 catgccccac ggcatcccct gagcgcgccc ttgtactttg acgaattcat ccagaagttg
      601 tcccagccct cctggccgtc cttggtgctg ctctacggcg attcggaagg ggtcatggcc
      661 gaggggctga cccgcctgcg ggaggccttc cgcaaaaggg cccccggcgg cacggtggac
      721 gtccgggagg gcggggaagg ggacctggcc ggccttttgg ccgaggccca gaccgcctcg
      781 ctcttcgccg accgcaggct gctggtgctg cggggcgccg gaaaggcgtt gggcgggcgg
      841 tccgaggccg cccaggccag gctggcggac tacgcccgcg accccaaccc ggacgccacg
      901 gtggtcttcg ccgccgaggg cctgcgcaag acgtccaaga tcgtgggctg gttcgagaag
      961 aacggctggg cggcgcaatg ctcggacatc ccggattgga agctcgggcc ctgggtgcgc
     1021 cagcaggccc gcgccctggg gctggagctg ggcgacgagg gcgcccaagc gctgctgcaa
     1081 aagaccggca cggacatcgc ctacctgaac cgggccctgg agcacctggc gctcttcatc
     1141 catcccgcgc gcaaaccctc cttgcaggac gtgctggacc tgcccatccc gggcgcggag
     1201 gcggagatct tccccttcct ggacgattgg ggcatgcggc gcaccgaaaa ggcgttgctg
     1261 ggcctgcggc gcatggcgcc gggcgcggag aacggcctgg tgttcatgct ctaccagcgg
     1321 gtgcgggacc tcttgggcgt ggcggcggga agggccgagg ggctggacca gtcccaattg
     1381 gcccaacggc tggggatcca cccattccgg gtgaagaacc tgtgggaaca gtcgtcccaa
     1441 tacacggcgg gggaactgcg gggggcgctg cgggacttgg tgcggatcca ggccggcttg
     1501 gtgacgggcc gcgtggggaa aagcgggctt tccaccctgg tggaatggtg gatcgtcaag
     1561 tgggggaaaa acccgttggc cgccgaggcg gccaacggcg ggtgactact tggacttgaa
     1621 ctgcttgtgg acgaaggacg acaggcgggc gatgttgcgg gaagcggtct ggcgtttcag
     1681 caccccgttg gaggcggcct tgtccagggc cttcatggcc aagcgcagct tggcggcggc
     1741 ctcttcctgg gtcttggcgg cgcggacggc cttgatggcc gtgcggatgc ggttcttctc
     1801 ggcggaattg cgggccgtgc ggcgcttgat gcggcggacg tccttgatcg acgacttgat
     1861 gatcggcatg gaatccttcc ttaaaggctt ggtgatcagt ggacgggtat cctaaacgaa
     1921 ggccaggggg ctgtcaagaa tggtccaaat gaagcggccc ggggggaaac cggggaggtc
     1981 cctgggcggg catttccgcc tggtctcgct ggccacgtcc ttgagccggg tcacgggctt
     2041 cgcccgggac at
//
LOCUS       DASTJJ010000300         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_288206, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000300 DASTJJ010000000
VERSION     DASTJJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288206"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFR02_03805"
     CDS             complement(<1..696)
                     /locus_tag="VFR02_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9869609.1"
                     /translation="MKPYKVVCLGNVLVDVLVKPVDRLPKPGSLLPVDHIELAMGGCA
                     SNTAIVLARLGVPTDLWGKLGKDDFGGYALKQLGREGVGVSGIRVSKKVSTSATIVVV
                     HSDAQRSFLHSMAANDEIGPKDLKLSRLSRFDHLHVGGYFLFPALDGPRMAKVLEEAR
                     HRGLTTSLDTAWDLQGRWLKTLKPCLPHLDYFMPSEREVKMLLGHAQPAQAARTFQKM
                     GAKNVVVKLGEKGS"
     gene            complement(828..>1050)
                     /locus_tag="VFR02_03810"
     CDS             complement(828..>1050)
                     /locus_tag="VFR02_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869610.1"
                     /translation="GAVVDHTGAPSVSQLTKGQIQSFQQFDDVAGLLQQKKQELMDDM
                     KNRALQAVPQDVVPGQQDVQNALGQKLGF"
BASE COUNT          195 a          328 c          329 g          198 t
ORIGIN      
        1 agagcccttt tcccccagct tcaccaccac attcttggcg cccatctttt ggaaggtccg
       61 ggccgcctgg gcgggttggg cgtggcccag gagcatcttc acttcccgct cgctgggcat
      121 gaaatagtcc aaatggggca aacagggctt caaggtcttg agccagcggc cctgaaggtc
      181 ccaggccgtg tccagcgagg tggtgaggcc ccggtggcgg gcttcttcca agaccttggc
      241 catgcggggc ccgtccagcg cggggaaaag gaaatagccc cccacgtgca ggtggtcgaa
      301 ccgggaaagc cgggaaagct tcaggtcttt cgggccgatc tcgtcattgg cggccatgga
      361 atgcaggaag gaccgttggg cgtcggaatg caccaccacg atggtggccg aggtggagac
      421 cttcttggag acgcggatgc ccgaaacgcc gaccccctcg cgcccaagct gtttgagggc
      481 gtagccgccg aaatcgtcct tccccaattt cccccaaagg tcggtcggga cgcccaggcg
      541 ggccaggacg atggcggtgt tggaggcgca gccgcccatg gccaactcga tgtggtcgac
      601 gggcaaaagg ctcccgggct tgggcagccg gtccaccggc ttgaccagca cgtccaccag
      661 cacgttcccc aggcagacga ctttataagg tttcaaatcc aaccccggaa agatctttca
      721 ccacgaagta cacgaaggac acaaagtgaa gatcatcatc cgagcaagat cctcacttcg
      781 tgcactttgt ggcccccgcc agggggctcg tggtgaaaga tgttgattta gaatcccagc
      841 ttttggccga gggcgttctg gacgtcctgc tggcccggca ccacgtcctg gggcacggcc
      901 tggagggccc ggttcttcat gtcgtccatc agctcctgct ttttctgctg caacaggccc
      961 gccacgtcgt cgaattgttg gaagctttgg atctgaccct tggtgagctg gctcaccgag
     1021 ggcgcgcccg tgtggtccac caccgcgccg
//
LOCUS       DASTJJ010000301         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1022041, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000301 DASTJJ010000000
VERSION     DASTJJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022041"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFR02_03815"
     CDS             <1..481
                     /locus_tag="VFR02_03815"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET9869611.1"
                     /translation="FGQFGQRGVGYDVKTLDAKMRQILGLHKAWRIALVGAGNLGMAL
                     LQYGGFAKAGFRIEAAFDVDPSKVGWELEGVKIWPTPSLRQVIREKKIEIGVLAVPAS
                     QAQRAAEMLVDAGVRAILNFAPCLITAPKSVLVREVDLAFELEHLTYFLEKSARDEK"
     gene            549..1202
                     /locus_tag="VFR02_03820"
     CDS             549..1202
                     /locus_tag="VFR02_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869612.1"
                     /translation="MDAQPIAAPAPRKRELKPFQAFRKVYHVVSASLLPLAYLFDPLR
                     QGETALRHTLLLTLGLSFAAFFLLDWLRLNDSRFNSQFMKLFSGLIRRTEQNKFNGAT
                     FLCFAFFLVILLFPKNVAVTAMLFLSLGDTAAELGGLYFGRLRIFRRSAEGTLSFFLV
                     AFVIAWALLGDWRVAFLGAAAGALIELFSFEVDDNLTVPIGSACAVWLIFSLFQNAK"
     gene            1481..1636
                     /locus_tag="VFR02_03825"
     CDS             1481..1636
                     /locus_tag="VFR02_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9869613.1"
                     /translation="MGSECEEVKRQLNLYRLGRLTEAEKAQVESHLLVCPECGFLLTL
                     VPSLTQD"
BASE COUNT          381 a          671 c          603 g          456 t
ORIGIN      
        1 tttcggccag ttcggccagc ggggcgtggg ctacgacgtg aagaccctgg acgccaagat
       61 gcggcagatc ctgggcctgc acaaggcctg gcgcattgcc ctggtggggg cgggcaattt
      121 gggcatggcg ctgctgcagt acgggggatt cgccaaggcg gggttccgca tcgaggcggc
      181 cttcgacgtg gacccctcca aggtggggtg ggaactggaa ggggtcaaga tatggcccac
      241 gccgtccctg cggcaggtca tccgggaaaa gaagatcgag atcggcgtgc tggccgtgcc
      301 cgccagccag gcccagcggg ccgccgagat gctggtggac gcaggcgtca gggccatcct
      361 caatttcgcg ccctgcctca tcaccgcgcc caagtcggtc ctggtgcggg aggtggacct
      421 ggccttcgag ctggaacacc tgacctattt cctcgaaaaa tccgccaggg acgaaaagtg
      481 acttggggcc ccccggggcc cgtgctagga tggcggcgtt ttcccaagcc ttcccgaagg
      541 aaaccgacat ggacgcccaa cccatcgccg ccccggcccc gcgcaagagg gaattgaagc
      601 ccttccaggc cttccggaag gtctaccacg tggtctcggc ctccctgttg ccgctggcct
      661 atctcttcga cccgctgcgc cagggtgaga ccgccctgcg ccacaccctg ttgctcaccc
      721 tggggctttc cttcgccgcc ttcttccttt tggattggct caggctcaac gacagccgtt
      781 tcaactcgca attcatgaag cttttctcgg ggctcatccg ccgcacggag cagaacaagt
      841 tcaacggcgc caccttcctt tgtttcgcct tcttcctggt catcctgctc ttcccgaaaa
      901 atgtggccgt cacggccatg ctcttccttt ccctgggcga caccgccgcc gagctgggcg
      961 gcctttattt cggccggctc cggatcttcc gccgctccgc cgagggcacc ctgtccttct
     1021 tcctggtggc cttcgtcatc gcctgggccc tcctggggga ttggcgggtg gcctttttag
     1081 gggccgccgc gggcgccctc atcgagttgt tctccttcga agtcgacgac aacctcaccg
     1141 tgcccatcgg ttcggcctgc gccgtttggc tgatcttctc ccttttccag aacgccaagt
     1201 aacccatccg gggatggcgg aaaaggaacg tcttccgcca gaattcccac aaaaattccc
     1261 ctccgtccaa gttccccatc ccatcccgga cgcctaaaaa caggacctga aatcgggatt
     1321 tgttgggaaa tcggggattt tccggaaaac taaaaacgac taaaaacaac gggctttccg
     1381 ttttccccct tggcacggat gatgcacttc aagagaatca tcttaactcg cttgcaggcg
     1441 acccacccta cgacacaacc gtcggaaagc gaggcacggt atgggatccg aatgtgaaga
     1501 agtcaaacgc cagctgaacc tttaccggct gggacgattg accgaggccg aaaaggccca
     1561 ggtcgaatcc cacctgttgg tctgcccgga atgcggtttc ctgctgacgc tggtaccctc
     1621 cctgacgcag gactgatctt tgggaagtcc gcgcccccgg ggccatgaaa ggcccggggg
     1681 cgtttttttg gaatcatgca tttcgcaaag caaggacaat aggaacactg ataaagcctg
     1741 atttttattt tcaaagatca gatttcatca gtttttatca ttgtttcaag ttctctttgg
     1801 cttgctttgc aaaagccata gttccgtttt ttgccggcct gagcgcgctt tccttgaatt
     1861 gacttttgtc cggccgccgg gcatgattgg cgccacttga ggccgcttgc cagggttttt
     1921 tgtccccgag ggggatgaaa agcccttctt ggcgttgggc ccatcctgcc aaggacaaga
     1981 cccatggccg ccgacaaccc catttccccg gacctggact ggccccaagc cagggccgcc
     2041 ctgaagcttt tggaagcgcg cgggggccgg tccctcatcc acggtctgcc gggctcggcc
     2101 aagggttttt t
//
LOCUS       DASTJJ010000302         1189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_899668, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000302 DASTJJ010000000
VERSION     DASTJJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899668"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFR02_03830"
     CDS             complement(<1..417)
                     /locus_tag="VFR02_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HET9869614.1"
                     /translation="MTLSDDLKGTVRAPEFPPGDAWLNTPQPLTLKGLKGKIVLLDFW
                     TYCCINCMHVIPDLKRLEEKYKDSLVVIGVHSAKFTTEKGTDAIREAVLRYGVRHPVV
                     NDADFKIWGAYGVRAWPTFALIDPEGKVVGLTSGEGV"
     gene            758..>1189
                     /locus_tag="VFR02_03835"
     CDS             758..>1189
                     /locus_tag="VFR02_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9869615.1"
                     /translation="MAAKRTAPSPEPKAPLDIPRIVLERMAFLLIKNAFKLREMTEEA
                     LKPHGLIGKHWGILTTLKEKGSLTQHDIGRCVHMDRSTMVMMIDDLEKAGLVERRPNP
                     ADRRAHSIHVTERGKVLWPELNRLVGSAEKRFLAVLSAKEQK"
BASE COUNT          220 a          388 c          385 g          196 t
ORIGIN      
        1 gaccccctcg ccggaggtga gccccaccac cttgccttca gggtcgatga gggcgaaggt
       61 gggccaggcc cggacgccgt aggcgcccca gatcttgaag tcggcgtcgt tgaccaccgg
      121 atggcgcacc ccgtagcgca gcacggcctc ccggatggcg tccgtgccct tttcggtggt
      181 gaatttggcc gaatgcaccc cgatgaccac cagcgaatcc ttgtactttt cctccaggcg
      241 cttgaggtcg gggatgacgt gcatgcagtt gatgcagcag taggtccaga agtcgagcag
      301 cacgatcttg cccttcagcc ccttcagggt caggggctgg ggcgtgttga gccaggcgtc
      361 gccggggggg aactcggggg cgcgcacggt gcctttcagg tcgtccgaca gggtcatggt
      421 gctcaggtct ccgaacgcgg ggggttcttg ggcggccagg gggccggcgg ccgccagggc
      481 caggatggcc ccccaaaaca gccgcgttaa atcgttccgc atgcctggat tttccttccc
      541 aagggtatcc acaatgggcc ggcctttcaa cggccgcccc cacccttggt gacaaatgga
      601 tacagggccg aaaagcattc ccggcgcccc tgggaaagaa taccccctgc cgggggtgga
      661 agtttcccgg gggggcgaaa ataaatccgg cggccctatt gacagtcggc ctgatagtca
      721 gtaacactga atatcagcta tccccgagga gttgcccgtg gccgccaaac gcaccgcccc
      781 gtcccccgaa cccaaagccc ccctggacat cccccggatc gtcctggagc gcatggcctt
      841 cctgctcatc aagaacgcct tcaaactgcg cgagatgacc gaggaggccc tgaagcccca
      901 cggcctcatc ggcaagcatt ggggcatcct gaccaccctc aaggaaaagg ggtccctgac
      961 ccagcatgac atcggccgtt gcgtgcacat ggaccggtcc accatggtga tgatgatcga
     1021 cgacctggaa aaggcggggc tggtggagcg ccgccccaac ccggcggacc gccgggccca
     1081 ctcgatccac gtgaccgagc ggggcaaggt cctttggccc gagctgaacc gcctggtcgg
     1141 gtcggcggag aagcggttcc tggcggtgct ttccgccaag gagcagaag
//
LOCUS       DASTJJ010000303         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1633772, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000303 DASTJJ010000000
VERSION     DASTJJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633772"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..>1000
                     /locus_tag="VFR02_03840"
     CDS             9..>1000
                     /locus_tag="VFR02_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9869616.1"
                     /translation="MSKPFDQADQNAVTAMRMLAVDATSKANSGHPGMPIGMAPAAHV
                     LWSRHLKHNPADPNWPDRDRFILSAGHGSMLIYALLHLHGYGLSLDELKNFRQWGSKT
                     PGHPEYGHTVGVETTTGPLGQGIATAVGFAMAERILAARFNEPGAELVGHKTWVIASD
                     GDLMEGVSHESCSTAGHLKLANLKVIYDDNHISIEGDTKLAFTEDVLKRFESYGWHTL
                     RVTDANDLEALDKAMAEASAETGRPSIIAVRSHIGYGTPLQDSAKVHGSPLSAENVAK
                     TKEFYKWPQEPTFHIPEDAKKRCLESVEKGKKLEAEWNAKKEAYGKAHADKLKL"
BASE COUNT          203 a          370 c          298 g          129 t
ORIGIN      
        1 agtttttcat gtccaagcct ttcgaccaag ccgaccagaa cgccgtcacc gccatgcgca
       61 tgctggcggt ggacgccacc tccaaggcca actcgggcca tccgggcatg cccatcggca
      121 tggcccccgc cgcccacgtc ctttggtccc gccacctgaa gcacaacccg gccgacccga
      181 actggccgga ccgcgaccgc ttcatcctgt ccgcgggcca cgggtccatg ctcatctacg
      241 ccctgctgca cctgcacggc tacggcctgt ccctggacga actgaagaac ttccgccagt
      301 ggggcagcaa gacccccggc caccccgagt acggccacac cgtgggcgtg gagaccacca
      361 cgggccccct gggacagggc atcgccaccg ccgtgggctt cgccatggcg gaacgcatcc
      421 tggccgcccg cttcaacgag ccgggcgccg aactggtggg ccacaagacc tgggtcatcg
      481 cctccgacgg cgacctgatg gagggcgtgt cgcacgagtc ctgctccacc gccgggcacc
      541 tgaaactggc caacctgaag gtgatctacg acgacaacca catcagcatc gagggcgaca
      601 ccaagctggc cttcaccgag gacgtcctga agcgcttcga gtcctacggc tggcacacgc
      661 tgcgcgtcac cgacgccaac gacctggagg ccctggacaa ggccatggcg gaagcctcgg
      721 ccgagaccgg ccgcccctcg atcatcgcgg tgcgcagcca catcggctac ggcacgccgc
      781 tgcaggacag cgccaaggtg cacggctccc cgctgtccgc cgagaacgtg gccaagacca
      841 aggaattcta caagtggccg caggaaccca ccttccacat ccccgaggac gccaagaagc
      901 gctgcctgga atcggtggag aagggcaaga agctggaagc cgagtggaac gccaagaaag
      961 aggcctacgg caaggcccac gcggacaagc tcaagctctt
//
LOCUS       DASTJJ010000304         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1265827, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000304 DASTJJ010000000
VERSION     DASTJJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265827"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFR02_03845"
     CDS             <1..1023
                     /locus_tag="VFR02_03845"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9869617.1"
                     /translation="GKHIVTANKAVLSVHWDELIQAARERKVDIYFEAAVCGGIPVIQ
                     AINDGLAPNNITSIYGIVNGTTNFILQKMTEEKKSFKDALQMAQKRGLAEADPTLDIN
                     GMDAAHKLAILASLAFSKRVKVEDIYVEGIERITVQDIEFAKEEFGRVVKLFAIAEVE
                     GDELEVRVHPTLIPEDHLLAKVDGEYNGVLVTGDFVGTTMYYGLGAGAKPTASAVMSD
                     LIYVCRAVHDNVAGETSNNWFLSDNPQIKRLRKMGDIKSRYYLKFSVVDQAGVLSAIA
                     RILGEHQISIASVIQKEQKKGDKVPVVIVTYEALEKNVQEALKKINGLPFVREETLLI
                     RMDRGE"
     gene            1023..2084
                     /gene="thrC"
                     /locus_tag="VFR02_03850"
     CDS             1023..2084
                     /gene="thrC"
                     /locus_tag="VFR02_03850"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9869618.1"
                     /translation="MAYWKGLIEEYRPYLPVTEATPVVTLKEGDTPLLYARHLSEKLG
                     NGLKLYLKFEGLNPTGSFKDRGMTMAISKALEDKSRIVMCASTGNTSASAAAYAAAAG
                     LKCYVVLPHGAIALGKIAQTLIHGAEVIAIEGNFDQALTLVREITTAHPVTLVNSANP
                     YRIEGQKTAAFEIVDTLGDAPEYHAIPVGNAGNITAYWKGYKEYQRKGLAKQLPRMCG
                     FQAAGSAPIVEKRVVEDPKTIATAIKIGNPYSWKQAEAARDESRGLIESVTDEEILEA
                     YNLLASQEGIFCEPASAASFAGVLKKNKEKFFVDGQRRVVCTLTGHGLKDPDTAVKQK
                     AKHHVVKPQAGEILKLMGL"
BASE COUNT          439 a          703 c          682 g          310 t
ORIGIN      
        1 ggcaagcaca tcgtgaccgc caacaaggcg gtcttgtccg tgcattggga cgagctcatc
       61 caggccgccc gcgagcgcaa ggtggacatc tatttcgagg ccgcggtctg cggcggcatc
      121 ccggtgatcc aggccatcaa cgacgggctg gcgcccaaca acatcacctc catctacggc
      181 atcgtgaacg ggaccaccaa cttcatcctg cagaagatga ccgaggaaaa gaagagcttc
      241 aaggacgccc tgcagatggc ccagaaacgc ggcctggccg aggccgaccc cacgctggac
      301 atcaacggca tggacgccgc ccacaagctg gccatcctgg cctccctggc cttctccaaa
      361 cgggtcaagg tcgaggacat ctacgtggag ggcatcgagc gcatcaccgt gcaggacatc
      421 gagttcgcca aggaagagtt cggccgggtg gtcaagctct tcgccatcgc cgaggtggag
      481 ggcgacgagt tggaggtgcg ggtgcacccc acgctcatcc cggaggacca cctgttggcc
      541 aaggtggacg gcgaatacaa cggcgtgctg gtgaccgggg acttcgtggg caccaccatg
      601 tattacggcc tgggggcggg cgcgaagccc acggcctcgg cggtcatgag cgacctcatc
      661 tacgtctgcc gggccgtgca cgacaacgtg gcgggcgaga cctcgaacaa ctggttcctg
      721 agcgacaacc cccagatcaa gcgcctgcgc aagatggggg acatcaagtc ccgctattac
      781 ctcaagttca gcgtggtgga ccaggcgggg gtgctttcgg ccatcgcgcg catcctgggc
      841 gagcatcaga tctccatcgc ctcggtcatc cagaaagaac agaaaaaggg cgacaaggtg
      901 ccggtcgtca tcgtgaccta cgaggcgctg gagaagaacg tccaggaagc cctgaagaag
      961 atcaacggcc tgcccttcgt gcgcgaggaa acgctcctca tccgcatgga ccggggggaa
     1021 taatggccta ttggaagggc ctcatcgagg agtaccgccc ctacctgccg gtcaccgagg
     1081 ccacccccgt ggtcaccttg aaggaaggcg acacgccgct cctctacgcc cgccacctgt
     1141 cggaaaagct cggcaacggc ctgaagctgt acctgaagtt cgagggcctc aaccccacgg
     1201 gttccttcaa ggaccggggc atgaccatgg ccatctccaa ggccctggag gacaagagcc
     1261 ggatcgtcat gtgcgcctcc accggcaaca cctcggcttc cgccgccgcc tacgcggccg
     1321 cggcggggtt gaagtgctac gtggtgctgc cccacggggc catcgcgttg ggcaagatcg
     1381 cccagaccct catccacggg gccgaggtca tcgccatcga ggggaacttc gaccaggccc
     1441 tgaccctggt gcgggagatc accacggccc acccggtcac cctggtgaac tcggccaacc
     1501 cctaccgcat cgagggccag aagaccgccg ccttcgagat cgtggacacc ctgggcgacg
     1561 cgcccgaata ccacgccatc ccggtgggca acgcgggcaa catcacggcc tattggaagg
     1621 gctataagga ataccagcgc aagggcctgg ccaagcagct gccgcgcatg tgcggtttcc
     1681 aggcggcggg ctcggccccc atcgtggaaa aacgcgtggt ggaggacccc aagaccatcg
     1741 ccacggccat caagatcggc aacccctatt cctggaagca ggccgaggcg gcccgggacg
     1801 agtcgcgggg cctcatcgag tccgtgaccg acgaggagat cctggaggcc tacaacctgc
     1861 tggcctcgca ggagggcatc ttctgcgagc ccgcctcggc ggcgtccttc gccggcgtcc
     1921 tcaagaagaa caaggaaaaa ttcttcgtgg acggccagcg gcgggtggtc tgcacgctca
     1981 ccggccacgg cctgaaggac ccggacacgg cggtcaagca gaaggcgaag caccacgtgg
     2041 tcaagcccca ggcgggcgag atcctgaagc tgatggggct ctgacatggc cctcatcgtc
     2101 cagaagttcg ggggctcctc ggtggccgac gccg
//
LOCUS       DASTJJ010000305         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1389089, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000305 DASTJJ010000000
VERSION     DASTJJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389089"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFR02_03855"
     CDS             complement(<1..232)
                     /locus_tag="VFR02_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869619.1"
                     /translation="MKTSKRLMQMVRMMGKPAPPGKPGESRPLLSISETLAKVQPTVW
                     VVLYGYLMAALVSYLAHRFFLWCPPYLQEIVKN"
     gene            complement(292..>1169)
                     /locus_tag="VFR02_03860"
     CDS             complement(292..>1169)
                     /locus_tag="VFR02_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869620.1"
                     /translation="RFLEAGGNLIIGPSYLDREVQPDWLVRLTGGWREGDHAGQAVAP
                     APAGEKPQLQLLDEIDVANDASEAGHQCSTDGAVTLDRSKLPELPRLTKMLEDDGRVF
                     KGYYQFSAKTEPGLPHRVWVRVNNGFNTAGTALLVKTDGGWVQAGVRTKNSGTDRDFL
                     TFYFEVPAPLIGSDHTLFRLADKGRDPINAYHLWVFGLNAPDGRRSLAQALGFQVHQT
                     LGRVDRGLIPLGGRWEEPLQLAEDPSQAALLAQRIGKGWLIRSQLNLGDSQGLMTALL
                     HPGPDVGPSPTASAP"
BASE COUNT          203 a          399 c          375 g          192 t
ORIGIN      
        1 ggttcttgac gatctcctgc aggtagggcg ggcaccagag gaagaaccgg tgggccaggt
       61 aggagaccag ggccgccatc aggtagccgt acaggaccac ccaaaccgtg ggttggacct
      121 tggccagggt ctcggagatc gacaacaggg ggcgggactc ccccggtttg cccggcgggg
      181 ccggcttccc catcatccgg accatctgca tcaggcgttt cgacgttttc acgggccctc
      241 ccccacgcgc atcactttaa caaatccaac gacaggtttc aaaagccatt cttaaggagc
      301 ggacgccgtc ggcgacggcc ccacgtccgg ccccggatgg aggagggccg tcatcaaccc
      361 ctgggaatcc cccaggttca attggctgcg tatcaaccag cccttgccga tccgctgggc
      421 caaaagggcg gcctgggacg ggtcctcggc cagctggagg ggctcttccc accggccccc
      481 caaggggatc aatccccggt ccacccggcc caaggtctgg tggacctgga accccagggc
      541 ctgcgccaag gatcgacgtc cgtccggggc gttcaacccg aagacccaaa ggtggtaggc
      601 attgatgggg tccctcccct tgtcggccag gcggaacaag gtatggtccg aaccgatgag
      661 cggggcgggg acctcgaaat aaaaggtgag gaaatcccgg tccgttcccg aatttttcgt
      721 ccgcacgccc gcctgcaccc agccgccgtc cgttttcacc agcagggcgg tccccgccgt
      781 gttgaaaccg ttgttcaccc gcacccagac ccggtgcggc aggcccggtt cggtcttggc
      841 ggagaactga tagtagccct tgaagacccg gccgtcgtcc tccaacatct tggtcaaccg
      901 gggcaactcg ggcagtttgg accggtccaa cgtgacggcc ccgtcggtgg agcactggtg
      961 gcccgcctcg gaggcgtcgt tggccacgtc gatctcgtcc aacagttgga gttggggctt
     1021 ttcaccggcc ggggccgggg ccaccgcctg ccccgcgtgg tccccttcgc gccagccgcc
     1081 ggtcaggcgc accagccagt cgggctgcac ctcccggtcc agatacgagg ggccgatgat
     1141 caggttgccg ccggcttcca ggaaccggc
//
LOCUS       DASTJJ010000306         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1511449, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000306 DASTJJ010000000
VERSION     DASTJJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511449"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..>1201
                     /locus_tag="VFR02_03865"
     CDS             314..>1201
                     /locus_tag="VFR02_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869621.1"
                     /translation="MPGPGGTGTAAGTAAGTSVAGGPIRRLLSLAAALTAAAARATRD
                     AASNGTGGGAAGPAGPVAGVAGPGGPGAGAGAWEGSSVVAAPLGVELVALSDGGVLEW
                     ARSVEQLSRWVQALQVQAAGELDSRAREGRYLCDGQATGMDVLSDVLHLSRAEAGRRL
                     GLAGHFLPATDALTTVATEPDQPVLAGAFFAGTTSIEQALVASHYTADAAHLARAGRT
                     TAELARELEGTMTTHAQEQNPDFLRRLGTRAITHIDPDGDTPTTGELTARQGIRFHRA
                     YRGMIRISGYTTIPQYEKIM"
BASE COUNT          181 a          390 c          439 g          191 t
ORIGIN      
        1 gccgatcgcg gtcagggcca tgccgccgaa aacagcgtat ttgtaggcag gaggagcggt
       61 ttcccaaaag tcattaagca caccccaagt ctaatggtgc aaaatccggg ctccgtctcg
      121 ggaacggcca tgccggattc ggtccgctgc caacctggag catgacgcat ctttgagact
      181 ggtttgggcc gtttttgtgt ggtggggcct ccacagggaa tgtgtgttct agggttgtca
      241 acattcgtgg gtttcccgcg gatgtttgtc ggtggctggt tgtagcgtgg tgtgcatgga
      301 gacaacggca agggtgccgg gcccgggagg caccggcacg gcggcaggga cggcggcagg
      361 gacgtcggtg gccggcgggc ctatccgccg cctgctcagc ctggccgccg ccctgaccgc
      421 ggccgcggcc cgtgccacca gggacgcggc cagcaacggc accggcggcg gggctgctgg
      481 tcctgctggt cctgttgccg gggttgctgg tccgggtggt ccgggtgccg gggcgggggc
      541 gtgggagggg tcctcggtgg tggcggcgcc gttgggggtg gagttggttg cccttagtga
      601 tgggggtgtg ttggagtggg cgcgcagtgt cgagcagctt tcccggtggg tgcaggccct
      661 gcaggtccag gcggccgggg aactggactc ccgggcgagg gagggccgct acctgtgtga
      721 cgggcaggcc acgggcatgg acgtgctcag tgatgtgctc catctcagcc gtgccgaggc
      781 cgggcgccgg ctgggcctgg ccgggcattt cctgcccgcc acggacgcgc tcaccaccgt
      841 ggccacggag ccggaccagc cggtcctggc cggggcgttc tttgccggca cgaccagcat
      901 cgagcaggcc ctggtcgcct cgcactacac cgccgacgcc gcccacctcg cccgtgccgg
      961 gcgcaccacc gcggaactgg cccgggagct ggaaggcacc atgaccaccc acgcccagga
     1021 acagaacccg gacttcctgc gccggctggg cacccgcgcg atcacccaca tcgaccccga
     1081 cggggacacc cccaccaccg gggaactgac cgccaggcaa ggcatccgct tccaccgcgc
     1141 ctaccgcggg atgatcagga tcagcggcta caccaccatc ccccaatacg aaaaaatcat
     1201 g
//
LOCUS       DASTJJ010000307         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_655480, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000307 DASTJJ010000000
VERSION     DASTJJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655480"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFR02_03870"
     CDS             complement(<1..450)
                     /locus_tag="VFR02_03870"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET9869622.1"
                     /translation="MILPLVKIGDPLLRRRSPAPRPGSPMLAKIVRDMTATMRKADGV
                     GLAANQVGLPLHLFVMESRGNRRYPKAASFPLQCYLGARIVRRSAKKVKGWEGCLSIP
                     GYRGLVPRHEWLIFTALTPQGQRVTRTVRGFEARVVQHEVDHLNGFFY"
     gene            587..1987
                     /locus_tag="VFR02_03875"
     CDS             587..1987
                     /locus_tag="VFR02_03875"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET9869623.1"
                     /translation="MPSFVQFPKDFLWGAATSSFQIEGSPLADGAAESNWYRWTKTPD
                     RIADGSDADLACDHYRRYASDVELMQGLGLKGYRFSLSWPRLVPARGAFNEKALDFYK
                     RLLDELRAKGIAPNVTLFHWETPVWAEGGWENRETALAFEEYARLIFAKLGDRVRTWA
                     THNEPVVTSQLGYLWGYFPPGKVDKAAFGRVTHHLNLSHGLALRAFRDGGYAGEIGLV
                     NALAPVHAATDKPEDVRQADRIDVLQNRIFLDPVQGRGYPAFFHEYSGAPAFSAEDLK
                     VIAQPVDFLGLNHYFPMVGRYAPGANVFDNDFRFPDGTPINDLDWAVDPDGLYRILVR
                     LWKQDGFKKIYITENGIPTRDSLRTPEETLEDDMRVHYMGTYLAAAQRAIAEGVPLKG
                     YYAWSLMDNFEWCHGYDPRFGIVHVDFKTQKRTLKKSAKWYQKAVEAGGFDLEALPKK
                     PGYRLLGAGGKANRSF"
     gene            2153..>3905
                     /locus_tag="VFR02_03880"
     CDS             2153..>3905
                     /locus_tag="VFR02_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9869624.1"
                     /translation="MDINQFTLNAQKALQSAQTLASDAGHAQVDNEHLFLALLQQEES
                     VVAPLLKKIGADPLTLQKSLQKDLEGLPKVSGDNQVYLAPRLGKVLDQAHKEADKMKD
                     EYVSTEHLLLALVEAPHASGQSDDWGVRGLVKRFHLTRDKVLSALSEVRGSTRVTDQN
                     PEDKYQALTKYGKDLTDLARRGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVG
                     KTAIAEGLAGRIVSGDVPEGLKGKKVIALDLGGMVAGAKFRGEFEDRLKAFLKEVTDS
                     QGQIILFIDELHTLVGAGKADGAMDASNMLKPALARGELRAVGATTLDEYRKYIEKDP
                     ALERRFQQVLVGEPSVPDTISILRGLKERYEIHHGVRIQDSALVAAAVLSNRYITNRF
                     LPDKAIDLVDEAASKLRMEIDSMPVALDEVERKMRQLEIEKQALQKEKDQASKDRLEK
                     LEKELANLKESSSHLKTRWQSEKKVIQDVRKLKEQLDGLKTDEAKYERAGDLNKVAEL
                     RYGQAPKLQAQLKEAEAKLTDLQKSGAMLKEEVTDEGIAEVVAKWTGIPVSKMMQGET
                     EKLIHMEAKLKERVVGQD"
BASE COUNT          759 a         1279 c         1269 g          598 t
ORIGIN      
        1 gtaaaagaaa ccgttcaggt ggtcgacctc gtgctgcacc acgcgggcct cgaacccccg
       61 caccgtccgg gtgacccttt gcccctgggg cgtgagggcc gtgaagatga gccattcgtg
      121 gcgggggacc aggccccggt aacccgggat gctcaggcag ccttcccagc ccttcacctt
      181 tttggccgaa cggcggacga tccgcgcgcc caggtagcat tgcaggggga aggacgcggc
      241 cttggggtag cggcggttgc cgcggctttc catgacgaag aggtgcaggg gcaggcccac
      301 ctggttggcg gccaggccca cgccgtcggc cttgcgcatg gtggcggtca tgtcccggac
      361 gatcttggcc agcatggggc ttccggggcg gggcgcggga gagcgtcgcc gcaggagggg
      421 atcgccgatc ttgaccaagg gcaggatcac ggagggcctt tcagggcttt tgccgatgat
      481 tttaccccga gcccccgcgg gggcaaaggc ccaagggcga ataataggtg aaggggcccc
      541 ggctctcctg atacactacg cctcattttg acctaagcga ggggccatgc cttctttcgt
      601 ccaatttccc aaggatttcc tctggggcgc cgccaccagt tccttccaga tcgagggctc
      661 gcccctcgcc gacggggccg ccgagagcaa ctggtaccgc tggaccaaga cccccgacag
      721 gatcgcggac ggcagcgacg ccgacctggc ctgcgaccat taccgccgtt acgcctccga
      781 cgtggagctc atgcagggcc tggggttgaa gggctaccgc ttctccctct cctggccgcg
      841 cctggtcccc gcacgggggg ccttcaacga gaaagccctg gacttctaca aacgcctgct
      901 ggatgaactg cgggccaagg gcatcgcgcc caacgtgacc ctcttccatt gggaaacacc
      961 ggtctgggcc gagggcggct gggagaaccg ggagaccgcc ctggccttcg aggaatatgc
     1021 ccggctgatc ttcgccaagt tgggcgaccg ggtgcgcacc tgggccacgc acaacgagcc
     1081 cgtggtgacc tcccaactgg gctacctctg gggttacttc ccgccgggca aggtggacaa
     1141 ggccgctttc ggccgggtga cgcaccactt gaacctgtcc cacggcctgg ccttgcgggc
     1201 cttccgggac gggggttacg ccggggagat cggcctggtg aacgccctgg ctcccgtgca
     1261 cgcggccacc gacaagccgg aggacgtccg ccaggccgac cgcatcgacg tgctgcagaa
     1321 ccgcatcttc ctggacccgg tccagggccg gggctatccg gctttcttcc acgaatattc
     1381 gggcgccccc gccttttcgg cggaggacct gaaggtcatc gcccaaccgg tggacttcct
     1441 gggcttgaac cactatttcc ccatggtggg ccgttacgcc cccggcgcga acgtcttcga
     1501 caacgatttc cgtttcccgg acggcacccc catcaacgac ctggactggg cggtcgaccc
     1561 cgacggcctt taccgcatcc tggtgcggct ctggaaacag gacgggttca agaagatcta
     1621 catcaccgag aacggcatcc ccacccggga ttcgctgcgc acgcccgaag agaccctgga
     1681 agacgacatg cgggtgcatt acatgggcac ctacctggcc gccgcccaac gggccatcgc
     1741 ggagggcgtg cccctgaagg gctattacgc ctggtccttg atggacaatt tcgaatggtg
     1801 ccacggctat gacccccgct tcggcatcgt ccacgtggac ttcaagaccc aaaagcgcac
     1861 cctcaagaag tcggccaagt ggtaccaaaa ggccgtcgag gcggggggct tcgacctgga
     1921 agcccttccg aaaaagccgg gctaccggct tttgggcgcc gggggcaagg ccaaccgcag
     1981 cttctaggat ccggaccctc ccggtccggg cttttcccgc cttttgcggc cctttctatg
     2041 accccggtca ttgaaagagg gcccttgccg ccttattttg ggctgggacc gcttggccca
     2101 tcgggcccgg ccggtccaac ttgaattcaa caattaaaga ggttcacccg atatggacat
     2161 caatcaattc accctgaacg cccaaaaggc cctgcagtcg gcccaaacgc tggcctcgga
     2221 cgccggccac gcccaggtcg acaacgagca cctgttcctg gccctgctcc agcaggaaga
     2281 gagcgtggtg gcgcccctct taaagaagat cggcgccgac cccctgacgc tgcaaaagtc
     2341 cctccaaaag gacctggaag gcctgcccaa ggtttccggc gacaaccagg tctacctggc
     2401 gccccgcctg ggcaaggtct tggaccaggc ccacaaggaa gcggacaaga tgaaggacga
     2461 gtatgtctcc accgagcacc tgcttttggc cctggtggag gcgccccacg cctccggcca
     2521 gtccgacgat tggggcgtgc gggggctggt gaagagattc cacctgaccc gcgacaaggt
     2581 gctgtcggcc ttgagcgagg tgcgggggtc cacccgggtg accgaccaga atcccgagga
     2641 caagtaccag gccctcacca agtacggcaa ggacctcacg gacctggccc gtcggggcaa
     2701 gctggacccg gtcatcggcc gggacgagga gatccgccgg gtcatccagg tgctgtcgcg
     2761 ccgcaccaag aacaacccgg tcctcatcgg ggagcccggc gtgggcaaga ccgccatcgc
     2821 ggaaggtctg gcgggccgca tcgtgtcggg cgacgtgcct gaggggctca agggcaagaa
     2881 ggtgatcgcc ctggacctgg gcggcatggt ggccggggcc aagttccgcg gcgagttcga
     2941 ggaccgcttg aaggccttcc tgaaggaggt caccgacagc caggggcaga tcatcctctt
     3001 catcgacgaa ctgcacacgc tggtcggggc cggcaaggcc gacggggcca tggacgccag
     3061 caacatgctc aagcccgccc tggcgcgcgg cgagctgcgc gcggtgggcg ccaccacgct
     3121 ggacgaatac cgaaaataca tcgagaagga cccggccctg gaacgccgct tccagcaggt
     3181 gctggtgggg gaaccctcgg tgcccgacac catctccatc ctgcggggcc tgaaggaacg
     3241 ctacgagatc caccacgggg tgcgcatcca ggactcggcc ctggtggccg ccgccgtgct
     3301 ctccaaccgc tatatcacca accgcttcct gccggacaag gccatcgacc tggtggacga
     3361 ggccgcctcc aagctgcgca tggagatcga ctccatgccg gtggcgctcg acgaggtgga
     3421 gcgcaagatg cgccaattgg agatcgagaa gcaggccctg cagaaggaaa aggaccaggc
     3481 ctccaaggac cgtctggaaa agctcgagaa ggaactggcc aacctcaagg aaagctccag
     3541 ccacctgaag acccgctggc agtccgagaa gaaggtcatc caggacgtgc gcaagctcaa
     3601 ggagcaattg gacggcctca agaccgacga ggccaagtac gagcgcgcgg gcgacctgaa
     3661 caaggtggcc gagctgcgct acggccaggc gcccaagctg caggcccaat taaaggaagc
     3721 cgaggccaaa ttgacggacc tgcagaagtc cggcgccatg ctgaaggaag aggtcaccga
     3781 cgagggcatc gccgaggtgg tggccaaatg gacgggcatc cccgtatcca agatgatgca
     3841 gggcgagacc gagaagctga tccacatgga agccaagctc aaggaacgcg tggtggggca
     3901 ggacg
//
LOCUS       DASTJJ010000308         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1145539, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000308 DASTJJ010000000
VERSION     DASTJJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145539"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFR02_03885"
     CDS             <1..769
                     /locus_tag="VFR02_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869625.1"
                     /translation="VSRAHPNLVRKMFELEVPEIYEGVVKIESVAREAGARTKISVRS
                     MGDDRVDAVGACVGVKGIRVQNIVDEVRGEKVDIVRWDEDPHNYLSNALSPAKISRII
                     FHTETPSAEVIVPNDQLSLAIGKKGQNARLAARLTGIKVDIKNEEQYAQEERARMDAH
                     FLEISQDAPAGDPQAPTDEDRKVAPPDEINEGAVQGESTAVTVDKDDSGAPIIPPTPK
                     MEPSEVTAQEAGPETQPEAEPEAVPEAAPAQTTEGEG"
     gene            779..>1558
                     /locus_tag="VFR02_03890"
     CDS             779..>1558
                     /locus_tag="VFR02_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HET9869626.1"
                     /translation="MSKRLFEFAKELGLTSKEVLERTKKLGFNIPSQLTVVDEKVQAD
                     VRRDLGLVADLSASVPSSPPVPAAPAASKGPTPLKLPPPGTRLGAPAPSSRTITRPAP
                     QHPSRSSAPKPATAPRKPSGPPRPMPVKTGGHGLAAGRPQLKPTAAPKAEVEEKAPAV
                     DPVTGEPLAQPKVLTKIKVTEGITVAELAQKLNVKPGVLIKELMDLKILATINQKMDM
                     TVVETLAGAHDAEVEVVPLYGSEMFQDSPDDPKDLVPRPPVV"
BASE COUNT          310 a          560 c          507 g          181 t
ORIGIN      
        1 cgtgagccgc gcccatccca acctggtgcg caagatgttc gagctggaag tgcccgagat
       61 ctacgagggc gtggtcaaga tcgagagcgt ggcccgggaa gccggcgccc gcaccaagat
      121 ctcggtgcgc tccatgggcg acgaccgcgt ggacgccgtg ggcgcctgcg tgggcgtcaa
      181 gggcatccgg gtgcagaaca tcgtggacga ggtgcggggc gagaaggtgg acatcgtgcg
      241 ctgggacgag gatccgcaca actacctgtc caacgccctg tcgccggcca agatctcccg
      301 catcatcttc cacaccgaga ccccctcggc cgaggtgatc gtgcccaacg accagctgtc
      361 gctggccatc ggcaagaagg gccagaacgc ccgcctggcg gcgcgcctga ccggcatcaa
      421 ggtggacatc aagaacgagg aacagtacgc ccaggaagag cgcgcccgca tggacgccca
      481 tttcctggag atctcccagg acgcccccgc gggggacccg caggccccca ccgacgagga
      541 ccgcaaggtg gccccgccgg acgagatcaa cgagggcgcc gtgcagggcg aatcgaccgc
      601 cgtgacggtg gataaggacg acagcggggc gcccatcatc ccgcccacgc ccaagatgga
      661 gccgtccgaa gtgacggcgc aggaagcggg accagaaacg caaccagaag cggagccaga
      721 agccgtgccg gaagccgccc cggcccaaac gaccgaaggg gagggttaac gaccgcatat
      781 gagcaaacgc ctttttgaat tcgccaagga actgggactg accagcaagg aagtgctgga
      841 gcggaccaag aaattaggat tcaacattcc cagccagctc accgtggtgg atgagaaggt
      901 gcaggccgac gtgcgccggg acctggggtt ggtggccgac ctttcggcca gcgtgccgtc
      961 ctcgccgccc gtgccggcgg cccccgcggc ctccaagggc cccacgccgc tcaaactgcc
     1021 gcccccgggc acccgcctgg gcgcccccgc gccctcctcg cgcaccatca cccggcccgc
     1081 gccccagcat ccgtcccgtt cctcggcccc caaaccggcc acggcccccc gcaaaccctc
     1141 cggtcccccc cggcccatgc cggtcaagac cggcggccat gggttggccg ccggccgtcc
     1201 gcagttgaag cccacggcgg cccccaaggc cgaggtggag gagaaggccc cggccgtcga
     1261 cccggtcacg ggcgaacccc tggcccagcc caaggtgctc accaagatca aggtgaccga
     1321 gggcatcacg gtggccgaac tggcccagaa gctcaacgtc aagcccggcg tcctcatcaa
     1381 ggaactgatg gacctcaaga tcctggccac catcaaccaa aagatggaca tgacggtggt
     1441 ggagacgctc gccggcgccc acgacgccga agtggaagtg gtcccgctct acgggtccga
     1501 gatgttccag gacagccccg acgatcccaa ggacctggtg ccccgcccgc cggtggtc
//
LOCUS       DASTJJ010000309         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_900895, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000309 DASTJJ010000000
VERSION     DASTJJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900895"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFR02_03895"
     CDS             complement(<1..567)
                     /locus_tag="VFR02_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869627.1"
                     /translation="MGRPVKRSLLLKTARLLAGYGLGTLCWVLGFHCLEPVEGGGLRL
                     DGRRWLRHGLKLGAWVTALGLLGRAWPAYGNWIMNVPILTVTLAFLVKGLIFLYVRVF
                     WPSKLVELVPGVRHFLCPQCLRRETFRFTPVSFRFGFFVTYLCRYCFCLVDGWGRQIF
                     FPAPFSPAAVRPFWARLVPVSAAVALAGW"
     gene            complement(662..>1268)
                     /locus_tag="VFR02_03900"
     CDS             complement(662..>1268)
                     /locus_tag="VFR02_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869628.1"
                     /translation="KSLDRDQIESLLGGTALTAKDYGVGSARLSSQVLSPSYNQPGAT
                     GAGVTITLGSGGAFVWDGRGDDGRILSPGNYYLELKSVQPNTAAQQVTWDIVVLPGSG
                     GPPGQTLLAPNPIRLDQTQTARFQVHLTSPLVDNVVVRLYTLAGERMDLELVNDPGNP
                     SQVTWNLAGTNIASGMYLGVVELRQGRNLLGRQVLKIAILH"
BASE COUNT          255 a          422 c          422 g          169 t
ORIGIN      
        1 ccaacccgcc agcgccacgg ccgccgaaac ggggaccaac cgggcccaaa agggccggac
       61 ggcggccggg gaaaaggggg ccgggaaaaa gatctggcgc ccccagccat cgaccaggca
      121 gaagcaatag cggcaaaggt aggtcacgaa gaaaccgaag cggaaggaca ccggcgtgaa
      181 acggaaggtc tcccggcgca ggcattgggg acagaggaaa tgccgaacgc ccggcaccag
      241 ttccaccaat ttggagggcc agaagacccg gacatagagg aagatcaggc ccttcaccag
      301 gaaggccagg gtgacggtca ggatcgggac gttcatgatc cagttcccgt aggccggcca
      361 agcccgcccc aacaggccca aggccgtgac ccaggccccc agcttcaacc cgtgccggag
      421 ccatcggcgc ccatccagcc tcaatccccc tccttccacc ggctccaaac aatggaaccc
      481 caagacccag cagagggtgc ccaggccgta gccggccaag agtcgggccg tcttgagcaa
      541 aagggatctt ttgaccgggc ggcccatggg gctccttgcg ggaaaacagg ggaatgggtg
      601 cggaaaacgg gacattatcc caaaaaaggg gcaaagggcc aaccaaatcg cccgggccgg
      661 actagtgcag gatggcgatc ttgaggacct gccggcccag caggttgcgg ccctggcgca
      721 gttccactac gcccaggtac atgccgctgg cgatgttggt ccccgccagg ttccaggtca
      781 cctgcgacgg gttgcccggg tcgttcacca gttccaggtc catccgctcc ccggccaggg
      841 tataaaggcg caccaccacg ttgtccacca gcgggctggt gagatggacc tggaaccggg
      901 ccgtttgggt ctggtccagc cggatcgggt tgggcgccag gagggtttgg cccgggggcc
      961 cgcccgaccc gggcaggacc acgatgtccc acgtgacctg ctgggccgcc gtgttgggtt
     1021 ggaccgactt gagctcgagg taatagttcc ccggcgagag gatccgcccg tcgtcccccc
     1081 ggccgtccca cacgaaggcg ccgcccgatc ccagggtgat ggtcacgccc gcgcccgtcg
     1141 cgccgggctg gttgtaggag gggctgagca cctgcgagga gaggcgggcg ctccccacgc
     1201 cgtagtcctt ggccgtcaag gccgtcccgc ccaacaggga ttcgatctgg tccctgtcca
     1261 ggctcttc
//
LOCUS       DASTJJ010000310         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_289627, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000310 DASTJJ010000000
VERSION     DASTJJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289627"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1472
                     /locus_tag="VFR02_03905"
     CDS             <1..>1472
                     /locus_tag="VFR02_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9869629.1"
                     /translation="IPEALRLADNLDVAQKGSHDWAISSRGFDTDSANKLLVLMDGRS
                     VYTPLFSGVFWDVQDYLLEDVDRVEVISGPGGTLWGANAVNGVINITTKDARDTQGTY
                     WEASGGSRFQDSLAARYGGRADSGLYYRVYGKYFALGNEDLASGGEASDAWNHKQGGF
                     RMDGEAGEDHFTLQGDLYGTTANLSSGGTAESDGGNLLGRWTKTFAADDDMGLQAYYD
                     RTYLYDPVPASVSSGGATLAPAGTLTDNLTTLDLDFHHRFALTRAQELTWGLGVRYTH
                     DTVTDAPGLAFDPSTLDQGLFSAFAQDEIGLWKDLTLTAGTKLEHNDYTGFEWEPSAR
                     LKWKLDGGQMLWTAVSRAVRAPSRVDRDILLPTPNFSPIVDDLLVGGKDFRSETLVAY
                     ELGYRAQAAPDLAASASAFFNNYDDIRSTSLSPPNAYSLPFPLYYANNLAAQTWGLEL
                     TADWQAAPGWRLHGGYDLLQESVWVKPGQFDYNNALNETA"
BASE COUNT          252 a          499 c          476 g          245 t
ORIGIN      
        1 cgatcccgga ggccctgcgc ttggccgaca acctggatgt cgcccagaag ggttcccatg
       61 actgggccat cagttcccgg ggcttcgaca ccgattcggc gaacaagctg ttggtgctga
      121 tggacggccg cagcgtctat accccgctct tttccggcgt cttctgggac gtgcaggatt
      181 atttattgga ggacgtcgac cgcgtggagg tcatcagcgg gccgggaggc accctgtggg
      241 gcgccaacgc ggtcaacggg gtcatcaaca tcaccaccaa ggacgcccgc gacacccagg
      301 gcacctactg ggaggcctcc ggcggcagcc ggttccagga ttccctggcg gcgcgttatg
      361 ggggccgggc ggattcgggc ctttattacc gggtctacgg caagtatttc gccttgggca
      421 atgaggacct ggcttccggc ggcgaggcgt ccgacgcctg gaaccacaag cagggcggct
      481 tccggatgga cggggaagcg ggagaggacc atttcaccct gcagggcgac ctctacggca
      541 ccacggcgaa cctgtcctcg ggcggcacgg cggaatcgga cgggggcaac ctcttgggcc
      601 gctggaccaa gaccttcgcg gccgacgacg acatgggcct gcaggcctat tacgaccgca
      661 cctaccttta tgatccggta ccggcctccg tgagctcggg cggggccacc ctggcgccgg
      721 cggggacctt gacggacaac ctgaccacgc tcgacctcga cttccatcat cgattcgccc
      781 tcacccgggc ccaagagctg acctggggct tgggcgtccg ctacacccac gacaccgtca
      841 cggacgcgcc cggattggcc tttgacccct ccaccctgga ccagggcctg ttcagcgctt
      901 tcgcccagga cgagatcggc ctttggaagg acctgaccct caccgcaggc accaagctgg
      961 agcacaacga ctacaccggc ttcgagtggg agcccagcgc gcggttgaaa tggaaattgg
     1021 atggggggca gatgctctgg acggcggtgt cccgggccgt aagggcgccc tcccgggtgg
     1081 accgcgacat cctgttgccc acgccgaact tctcgcccat cgtggacgac ctgttggtgg
     1141 gggggaagga tttccggtcc gagaccctgg tggcctatga gctgggttat cgggcccagg
     1201 ccgcccccga cctggccgcc tcggcctcgg ccttcttcaa caactacgac gacatccgca
     1261 gcaccagctt gagcccgccc aacgcctatt cgctgccgtt ccccctctat tatgccaaca
     1321 acctggcggc ccagacctgg gggttggaac tgacggccga ctggcaggcg gcgccgggct
     1381 ggcgcctgca cgggggttac gacctcctgc aggaatcggt ctgggtcaag cccggccagt
     1441 tcgactacaa caacgccctc aacgagaccg cc
//
LOCUS       DASTJJ010000311         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_656127, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000311 DASTJJ010000000
VERSION     DASTJJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656127"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFR02_03910"
     CDS             <1..446
                     /locus_tag="VFR02_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9869630.1"
                     /translation="TLSTGQTFTWDGRNDAGQIVSPGQYYFEVKSTDGQGGQSEFTQS
                     VVVLHGGGDLEKTPVVVYPNPTNPSTYGNLVTFKDPSGSLNLSVKLYTLAGERVPLPA
                     LTAVGGGLYTLDISKLASGLYIADIELSDASGGRQRQITQLAVLR"
     gene            complement(510..1526)
                     /gene="bioB"
                     /locus_tag="VFR02_03915"
     CDS             complement(510..1526)
                     /gene="bioB"
                     /locus_tag="VFR02_03915"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET9869631.1"
                     /translation="MPLSGLTSTLWDELAQKAIEKVPPTPEEALSILKAPQEETLSLV
                     DAAYRVRRHFHGNKVRIHVLQNAKSGACPEDCGFCSQSSKYQTPASTYPMLDVEELVE
                     GARKAKSAGAWKYCMVTATRGPSNRDLDVICEATRRIKSEGGIKVCTSLGILTPEKAR
                     RLAEAGVDRFNHNLETSERHYAKIVSTHSYQDRVATTQYAREAGMEICSGGIIGLGEE
                     TEDILELCYALRRVGATSVPVNFLDPRPGTPLGGGTPVDPYYALRVLCFFRFVHPDVD
                     LRAAGGREITFRSLQPLVLYVVNSIFTNGYLTTGGQGENADHRMIQDMGMVPEVVGGE
                     FNAG"
     gene            2156..>3979
                     /locus_tag="VFR02_03920"
     CDS             2156..>3979
                     /locus_tag="VFR02_03920"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET9869632.1"
                     /translation="MLFAFFAAGSLWAAPTVTVSLSSTTVAPGGILGVTVVYCDTNGQ
                     DFLFGALESSANTTLQGCGVAGQHYLLDGNTGAGNPVTATQDDSSTFNGWGGQSFPFG
                     ATCPAASVTQVFGLTIPSTMTAGSYNLVIADQEYGIQCNNFGGATQKSVAFTIGTGPT
                     SMPTATPTVTRTPTITRTPTITMTPTITGTPTRTGTPTQTATITLTPVNTFTVTPTFT
                     ATPSCYSGDLYVNVGCSSCGALTFTSIQGAINVACSGATIILLNNDTESVSFGSSQNI
                     TLNIMADPNAAAGSITWNAGSSASGLFSAFNNDQSPLTISGLTLNQPNSTGSLFTWNN
                     MNNGKVNFTNDIMMSTGSTTSPLITSNSANQNEGFSFDQVSILGNATTPAGIYLPSGN
                     NGNSVYMQNTVVAGFTGGYGIELSGNSYATIYNSTIADNNIGFYQADVNDASTVVNTA
                     FANLTTDVSASKLGGFTYDAYVKTTLSGTGDFSITTAAFVSFGSDYHLATGSTLIDAG
                     TNDGITIDRDYNTRPQCVTWDVGAYEATNCLSPTPTPTRTSTFTPTYTPTLTPTRTPT
                     FTPTTTPTITKTFTPTVTLTQTFTPTYTPTITLTPTSTPTFT"
BASE COUNT          799 a         1271 c         1152 g          757 t
ORIGIN      
        1 tgaccctgtc cacggggcag accttcacct gggacgggcg caatgacgcg ggacagatcg
       61 tcagtccggg gcagtattat ttcgaggtca aatcgacgga tggacagggt gggcagtctg
      121 aattcaccca atcggtggtg gtgttgcacg gcggcggcga cctggagaag acccccgtgg
      181 tggtctaccc caaccccacc aacccctcga cctatgggaa cctggtcacc ttcaaggatc
      241 cctcggggtc gttgaacctc tcggtgaagc tctatacctt ggcgggggaa agggtccccc
      301 tgccggcctt gaccgccgtc ggcggagggc tctatacctt ggacatctcc aagctggcca
      361 gcggccttta tatcgcggac atcgaattat ccgacgcgtc gggcggccgt cagcgccaga
      421 tcacccagtt ggccgtcctg cgctgaaccg acatccccgg aaataaaaaa ggcccctctc
      481 ggggcctttt ttatttctcc tgatcggcgt tatccggcgt tgaactcgcc cccgaccacc
      541 tcgggcacca tgcccatatc ctggatcatg cggtggtcgg cgttctcccc ctgtcccccg
      601 gtggtcaggt agccgttggt gaagatggaa ttgaccacgt agagcaccaa gggttggagc
      661 gaccggaagg tgatctcccg tcccccggcg gcccggagat ccacgtcggg atggacgaac
      721 ctgaaaaagc aaagcacccg cagggcgtaa taggggtcca cgggggtgcc cccgcccagg
      781 ggcgtgcccg gccggggatc caggaaattg accggcaccg acgtggcgcc gacccgccgc
      841 aaggcgtagc aaagttccag gatgtcctcc gtttcctcgc ccaggccgat gatgcccccg
      901 gaacaaattt ccatgccggc ttcccgcgcg tattgggtgg tggccacccg gtcctgatag
      961 gaatgggtgg agacgatctt ggcgtagtgg cgctcggaag tttccaggtt gtggttgaag
     1021 cggtccacgc cggcttcggc caggcggcgg gccttctcag gggtcaggat ccctaaggac
     1081 gtgcagacct tgatgccgcc ctcggacttg atgcggcggg tggcctcaca aatgacgtcc
     1141 aggtcccggt tgctgggccc ccgggtcgcc gtcaccatgc agtatttcca ggccccggcg
     1201 gacttggcct tgcgggcgcc ttccaccaat tcttccacgt ccagcatggg gtaggtggag
     1261 gcgggggtct ggtatttgga ggattgggaa caaaagccgc agtcttccgg gcaggcgccg
     1321 gacttggcgt tctggagcac gtggatgcga accttgttgc cgtggaaatg ccggcgcacc
     1381 cgataagccg cgtccacgag ggaaagggtc tcttcctggg gggccttcag gatggagagg
     1441 gcttcctcag gggtgggcgg gaccttttcg atggcttttt gggccaactc gtcccacagg
     1501 gtggaggtga ggccggacag gggcattcag gatcctttac gggagggtgg ggcggcaggc
     1561 gggaagtata acaaagcgac cagggacttt ccacgccgtt gcctggagcg gtttaccacc
     1621 ggtcctgttc tcaagaaaac gcttggcaag gaccttggac gatcaaaaag atcacctcat
     1681 gctcgtgatc caagggcaag ctgggtaggg tgatggtcag gggagggttt ggatggtttc
     1741 aggttaaagt aattttggcg gcaagaggaa aaaagtatga attttaaagg gtttttgggc
     1801 caaaaagagg gtagttttcg gtcgtagaag tggtaaactt tgggttggaa atgacgttag
     1861 gtttaatgtg aaagcttcaa caacgaagcc cacatttcca aatttgccgg tttgccgacc
     1921 gtcaaaaaag atgcggaaat ttggaatggt attttgaacg ctcgccgctt gtctgcctat
     1981 ggatgcagtt agtctctcgt tccttttttc ggaaattttt tttttcgcgt tacatgaaaa
     2041 cgttatcccg gttaaaaact ttttaaccgg tccactgaat gaaagcgagc tcatggtcgg
     2101 atcgagtgtg gtgacaacgg aaaggggact tcgccggctt ttgaaagccg gatggatgct
     2161 ctttgccttt ttcgccgccg gctccctctg ggccgcgccg acggtgaccg tttcgctttc
     2221 cagcaccacg gtggcgccgg gcgggatctt gggcgtcacg gtggtctatt gcgataccaa
     2281 cggccaggac tttttgttcg gggctttgga aagctcggcc aacaccacgc tgcagggttg
     2341 cggggtggcg gggcagcatt acctgctcga cggtaacacg ggcgcgggaa atcccgtgac
     2401 cgccacccaa gacgattcaa gcactttcaa cggttgggga gggcagagct tccccttcgg
     2461 cgcgacctgc ccggccgcgt cggtcaccca ggtcttcggc ctgaccatcc cctccaccat
     2521 gaccgccggc agctataacc tggtgatcgc cgaccaggaa tacgggatcc aatgcaataa
     2581 cttcggcggc gcgacccaaa agtcggtggc tttcaccatc ggcacggggc cgaccagcat
     2641 gccgaccgcc acgccgaccg tcacccggac gcccaccatc acccgcacgc ccaccatcac
     2701 catgacgccg accatcaccg gcacccccac ccgcacgggg acgccgaccc agaccgcgac
     2761 catcaccttg accccggtca acaccttcac cgtcaccccg accttcacgg ccaccccttc
     2821 ctgctattcg ggcgaccttt acgtcaacgt gggatgttcg tcctgcgggg ccttgacctt
     2881 cacgtccatc cagggcgcca tcaatgtggc ctgctcgggg gcgaccatca tcctcctgaa
     2941 caacgacact gagtcggtgt cgttcggcag cagccagaac atcaccctca acatcatggc
     3001 cgaccccaac gcggcggcgg gttccatcac ctggaacgcc gggtcctccg ccagcggcct
     3061 tttcagcgcc ttcaacaacg accagagccc cttgacgatc tccggcctca ccctcaacca
     3121 acccaacagc acgggttcgt tgttcacctg gaacaacatg aacaacggca aggtgaattt
     3181 caccaacgac atcatgatga gcaccggcag caccacgagc cccctgatca cctccaacag
     3241 cgccaaccag aacgaggggt tctccttcga ccaagtctcc atcctgggca acgccaccac
     3301 gccggcgggc atctacctgc ccagcggcaa caacggcaat tcggtctata tgcagaacac
     3361 cgtggtggcg gggttcacgg ggggctatgg gatcgagctt tccggcaaca gctacgccac
     3421 catctacaac tccaccatcg cggacaacaa catcgggttc taccaggcgg acgtcaacga
     3481 cgccagcacg gtcgtcaaca ccgccttcgc caacctgacg acggatgtga gcgcctccaa
     3541 gctgggcggg ttcacctacg acgcctacgt caagaccacc ctttcgggaa cgggggactt
     3601 cagcattacg accgcggctt tcgtgagctt tgggtccgac taccacctgg ccacgggatc
     3661 cactttgatc gacgctggca cgaatgacgg gatcaccatc gaccgggact ataacacccg
     3721 gccccaatgc gtgacctggg atgtgggcgc ctacgaggcg accaactgcc tgagccctac
     3781 ccccacgccc acccgcacgt cgacgttcac tccgacctat acgcccaccc tgacgccgac
     3841 ccggaccccc acctttaccc cgaccacgac gccgacgatc acgaagacct ttacccccac
     3901 ggtcacgttg acccagacct tcaccccgac ctatacgccc acgatcaccc tgaccccgac
     3961 ctccacgccg accttcacc
//
LOCUS       DASTJJ010000312         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1512769, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000312 DASTJJ010000000
VERSION     DASTJJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512769"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFR02_03925"
     CDS             <1..494
                     /locus_tag="VFR02_03925"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9869633.1"
                     /translation="GLYGPLSQAARNPAKLLALFFKGRLPVLYAGQDHLDAVGLRWKT
                     QFNENAKQIALFNVVPEMNHNEILGYSLPEPLTRKMAVALLGHPGDHPQVRRRFDLLK
                     GMAAPKAAGVTQVGPKGRSRLAQMASLVYLGDFASVYLAYLKGVDPTPTGLIDELKRR
                     LAR"
     gene            604..>1744
                     /locus_tag="VFR02_03930"
     CDS             604..>1744
                     /locus_tag="VFR02_03930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9869634.1"
                     /translation="MESFQELGLPEFLGKSLQQMNFTQPTPVQAQAIPAALQGRDVVA
                     SAQTGTGKTAAFGIPLLTFLSKHPDQGALVMAPTRELAAQVLEVLRKLSGSHPLGGSV
                     LLIGGANMGGQINALLGKPRLIIGTPGRIIDHLNRGSLNLSKAGFLVLDEADRMLDMG
                     FEPQLEEIRKRLPTERQTLLFSATFPPDIQALGARYLKSPLRITVEGGGRPVEKINQM
                     ILRTTEPRKFEELAAELKERQGSVVVFARTQHRVERLLGKFKDAGFKATQVHGGRTQS
                     QRRQALEQFRQGSYRILVATDVAARGLDIHHIAHVVNYDLPRNPEDYIHRVGRTARAG
                     AQGDSLSLVTPEDRPLWESILRLIQVPRDSIQVVPSRFAAPGPEPS"
BASE COUNT          292 a          617 c          569 g          266 t
ORIGIN      
        1 ccggcctgta cggacctttg tcccaggcgg cccggaaccc cgccaagttg ctggccctct
       61 ttttcaaggg aaggttgccg gtcctctacg cggggcagga ccacctggac gcggtgggcc
      121 tgcggtggaa gacccagttc aacgagaacg ccaagcagat cgcccttttc aacgtggtcc
      181 ccgagatgaa ccacaacgag atcctgggct attccttgcc ggaaccgctg acccggaaaa
      241 tggccgtggc gttgttgggg catccggggg accaccccca ggtgcgccgc cgcttcgacc
      301 tgctgaaggg catggcggcc cccaaggccg cgggcgtcac ccaagtgggg cccaagggcc
      361 ggtcgcggtt ggcccagatg gcctccctgg tctacctggg ggatttcgcg agcgtctacc
      421 tggcctacct gaagggcgtg gatcccaccc cgaccggcct gatcgacgaa ttgaaacgca
      481 ggctggcccg gtaaagcccg ccataagcgc cgccggatag ggcgctgacg ccacccggcc
      541 ccggccgggg gtgaactttg gcggccgcaa ggcgcgccgc ctcccagaac caaaaggaat
      601 aaaatggaaa gctttcaaga gttgggactg cccgaatttt tggggaagtc cctgcagcag
      661 atgaatttca cccagcccac gcccgtgcag gcccaggcca tcccggcggc cctgcaaggg
      721 cgggacgtgg tggcctcggc ccagacgggc acgggcaaga ccgcggcctt cggcatcccc
      781 ctgttgacct tcctgtccaa gcaccccgac cagggcgcgc tggtcatggc ccccacccgg
      841 gagctggcgg cccaggtgct ggaggtgttg cgcaagcttt ccgggtcgca ccctttgggt
      901 gggtccgtgc tgctcatcgg cggcgccaac atgggcggcc agatcaacgc cctcttgggc
      961 aagccacggc tcatcatcgg cacgccgggg cgcatcatcg accacttgaa ccgcggttcg
     1021 ctcaacctgt ccaaggccgg cttcctggtg ctggacgagg ccgaccgcat gctggacatg
     1081 ggtttcgagc cccagttgga ggagatccgc aaacgcctgc ccaccgagcg ccagaccctg
     1141 cttttttccg ccaccttccc gcccgacatc caggccctgg gcgcccgcta cctgaagtcg
     1201 cccctgcgca tcacggtgga aggcggggga cggccggtgg agaagatcaa ccagatgatc
     1261 ctgcgcacca ccgagccgcg caagttcgag gaactggccg ccgagctcaa ggaacgccag
     1321 gggtcggtgg tggtcttcgc ccgcacccag caccgggtgg agcgcctgtt gggcaagttc
     1381 aaggacgccg gcttcaaggc cacccaggtg catgggggcc gcacccagtc ccagcggcgc
     1441 caggcgctgg agcagttccg ccagggcagc taccggatcc tggtggccac cgacgtggcc
     1501 gcccggggcc tggacatcca ccacatcgcc cacgtggtca attacgacct gccccgcaat
     1561 cccgaggact acatccaccg cgtgggccgc acggcccgcg ccggggccca gggggattcc
     1621 ctgtccctgg tcacccccga ggaccggccc ctgtgggaat ccatcctgcg cctgatccag
     1681 gtgccgaggg attcgatcca ggtggtgccg tcgcgcttcg cggcgcccgg ccccgagccg
     1741 tcct
//
LOCUS       DASTJJ010000313         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_44731, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000313 DASTJJ010000000
VERSION     DASTJJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44731"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..508)
                     /locus_tag="VFR02_03935"
     CDS             complement(101..508)
                     /locus_tag="VFR02_03935"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HET9869635.1"
                     /translation="MQELHLNLWVVLAAAVVKFLLGGLWYSAIFQKPWMKEMGFKPKD
                     MKKGMGARSMAIEFVGNLLMAFVLAHAIRYAGVHGPVDGLCVAFFNWLGFVAVVQIHS
                     VIFEMKSWRLFFINAGFQFTGMLLMGVILALWV"
     gene            complement(610..990)
                     /locus_tag="VFR02_03940"
     CDS             complement(610..990)
                     /locus_tag="VFR02_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869636.1"
                     /translation="MENEGYVPGVCNIGPQERVLRRAVGFLCVGFFTVLLLAFHFTHA
                     PYSLRLVLLLPAFIGSVGFIQDSMRFCVNFGFRGLYNLTRSAGVTMGVAQAEFRSQDR
                     EKAVEILCYSLAAALVATYLALLV"
     gene            complement(1404..2318)
                     /locus_tag="VFR02_03945"
     CDS             complement(1404..2318)
                     /locus_tag="VFR02_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9869637.1"
                     /translation="MGFLDKIEMGVGTWAWGDGAVWGFGKGYGAGDLKEVFDLNLAAG
                     VTFFDTAEVYGDGKSESYLGGFLKTVPDKAVHTATKFMPFPWRVSTSHLLKSLRASLK
                     RLDLPRVDLYQVHFPMPFRSIEAWMEPLAEAVKEGLAAEVGVSNYSRSQMLRARDALG
                     RRGVPLASNQVQLSLARRKNEFNGVLETCRAEGIRLIAFSPLGMGMLSGKYGPKNPPR
                     GPRRIFYFGQLGRVEKLVGLLREIGRAQGGKSPNQVALNWLIGKGALPIPGAKTAAQA
                     RENLGALGWRLSPDQMARLDEASRAFTL"
     gene            2537..>3202
                     /locus_tag="VFR02_03950"
     CDS             2537..>3202
                     /locus_tag="VFR02_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869638.1"
                     /translation="MLKYLSAYFMALFFCASCLQAQGLTGYSLLKTALTPDVSHPDRY
                     TYEWIAANHTPAAGSFRIIDHLSGGDELFSVTAQDGMTPDPAAQPILPGMGPAAVTLG
                     PFPIAAGSSVTFRVSGSRAPGSVPETFAILGQPAGASADSVGVSFGGDTPTPEGRTTV
                     TAAPNVSTGDSPIRFLFDLTSPAHVWLTLYTISGERVYDADEEAGAGTGALIWTLDNN
                     GRQK"
BASE COUNT          642 a         1016 c          965 g          579 t
ORIGIN      
        1 aagggcggaa atcgggtttt taaaggggcg gcggcccatg ggccgggcct agttcaagac
       61 ccaagaagcg gaagatcttc cgccgcagaa aggttggccg tcaaacccag agcgccagga
      121 tgacgcccat cagcagcatg ccggtgaact ggaagccggc gttgatgaag aagagccgcc
      181 aagacttcat ctcgaagatc acgctgtgga tctgcacgac cgccacgaaa cccagccagt
      241 tgaagaaggc cacgcagagg ccgtccaccg gcccgtgcac gcccgcgtaa cggatggcgt
      301 gggccaagac gaaggccatc aacaggttcc cgacgaactc gatggccatg gaccgggcgc
      361 ccatgccttt tttcatgtcc ttgggcttga agcccatttc cttcatccag ggcttttgga
      421 aaatggccga ataccacagc ccgcccagga ggaatttcac cacggcggcg gccaagacca
      481 cccacaggtt caaatgcagt tcttgcatga cggctccttc aacaaggatg acgacaaggg
      541 catcctaacc gggttggggg gcttgtcaat tctggctttg taaaatcggg gagggggccg
      601 gcgtccgggt cagaccaaaa gggccagata ggtggccacc agggcggcgg ccagcgaata
      661 acagaggatc tccacggctt tttcccggtc ttggctgcgg aactcggctt gcgccacgcc
      721 catggtgacc ccggcggacc gggtcaggtt gtagaggccc cggaagccga aattcacgca
      781 gaagcgcatg ctgtcctgga tgaagcccac gctgccgatg aaggcgggca acagcaggac
      841 caagcgcagc gagtagggcg cgtgggtgaa atggaaggcc aataggagga cggtgaaaaa
      901 acccacgcag agaaagccca cggcccggcg cagcacccgt tcttgggggc cgatgttgca
      961 gacgcctggg acataacctt cattttccat ggctttatcc taggaaggcc cccgcgcgcg
     1021 cgcaagggaa aagcggggta gcgtcccaat ttgaaagact tagaagtcat ttcaaaacct
     1081 cttttaccgc agagacgcag aggcgcaaag tgaagataag gggcgaaaaa ccggggaaat
     1141 aaacattatt tttcctgcgt ctccgcgtct ctgcggttga aaattttcgc atttagggtt
     1201 ttgaaacagg ttcttaattt tccccctatg ccgacatcct tcgaatatcc cagggaagtc
     1261 ggcataaggc ccttggcgca cccgaaccct cttcctcgga ggggggaggg aatggaaagc
     1321 cgacgctgaa gctctccctc tcccttgacg ggagagggcg gggtgagggt ggatcgaacc
     1381 gaggcattat cgaaaagcgg ggatcaaagg gtgaaggcgc ggctggcctc gtcgagccgg
     1441 gccatttggt ccggcgaaag gcgccatccc agggcgccca ggttctcccg ggcctgggcc
     1501 gcggtcttgg cgcccgggat gggaagcgcc cccttgccga tgagccagtt cagggccact
     1561 tggttggggc ttttgccgcc ctgggcccgc ccgatctccc gcagcagccc gacgagtttt
     1621 tccacccggc ccaattggcc gaaatagaag atccggcgcg ggccgcgggg cgggttcttc
     1681 gggccgtact tgcccgacag catgcccatc cccaaggggc tgaaggcgat gagccggatg
     1741 ccctcggcgc ggcaggtttc cagcaccccg ttgaactcgt tcttgcgccg cgccaggctc
     1801 agctgcacct ggttggaagc caagggcacg ccccggcgcc ccagggcgtc ccgggcccgc
     1861 agcatctggg agcggctgta attggagacc ccgacctcgg cggccaagcc ttccttcacg
     1921 gcctcggcca gcggctccat ccaggcctcg atggaccgga aaggcatggg gaaatgcacc
     1981 tggtagaggt ccacccgggg aaggtccagc cgtttcaggc tggcgcgcag cgatttcagc
     2041 aggtgggaag tggagaccct ccaagggaag ggcatgaact tggtggccgt atggaccgcc
     2101 ttgtccggga cggtctttaa aaagccgccc aggtagcttt cagatttgcc gtcgccgtag
     2161 acctcggcgg tgtcgaagaa ggtcaccccg gccgccaggt tcaggtcgaa aacctccttt
     2221 aagtcccccg cgccgtagcc cttgccgaag ccccagacgg ccccgtcccc ccaagcccag
     2281 gtgccgaccc ccatctcgat cttgtccaaa aagcccatgg ttcccctccg gaaagggtct
     2341 ccaaccaaag gccggagacg cgcgacataa aaacaacagt ttttatttta atgccatttt
     2401 tacactaaaa cgtttgtccg ttctgacacg ccttgttata ttccgcctaa caaattttcc
     2461 cggttgtttg cgctggatct ccgggacgca tcgtcgggcc ttgtgtcgaa tataaaagac
     2521 ggtccagggg caacccttgc tcaagtatct ttccgcctat tttatggcct tgttcttctg
     2581 cgcttcctgc ctgcaagccc aaggcttgac gggttattcc ctactgaaga ccgccctgac
     2641 gcccgacgtt tcccatcccg atcgatacac ctatgaatgg atcgcggcca accacacccc
     2701 ggccgccgga agtttccgca ttatcgacca tttgtccggg ggcgacgaac ttttttccgt
     2761 cacggcgcag gatgggatga cccctgaccc ggccgcccaa cccatccttc ccggcatggg
     2821 cccggccgcc gtgaccctcg ggcctttccc catcgccgcg gggtcttccg tcacgttccg
     2881 ggtctccggg tcccgcgcgc ccgggtcggt cccggagacc ttcgcgatcc tcggacaacc
     2941 ggccggggcc tccgccgatt cggtcggcgt ttccttcggc ggggataccc ccacgcccga
     3001 gggccggacc accgtgaccg ccgcgcccaa cgtctccacc ggggactcac cgatccgctt
     3061 cctcttcgac ctcacctcgc ccgcccacgt gtggctgacc ctttacacca tctcggggga
     3121 acgggtctat gacgccgacg aagaagccgg ggccgggacg ggggcgctca tttggaccct
     3181 ggacaacaac ggccgccaaa ag
//
LOCUS       DASTJJ010000314         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1635373, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000314 DASTJJ010000000
VERSION     DASTJJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635373"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..806
                     /gene="flgF"
                     /locus_tag="VFR02_03955"
     CDS             21..806
                     /gene="flgF"
                     /locus_tag="VFR02_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HET9869639.1"
                     /translation="MPSGLYIAASGMNATMTQQDVLANNLANVNTVGFKGNRTVDAAF
                     PTYLIARLNDQRLKTPDGDLDLRPTIGLMGGGVIPAATPVDYSQGSRVQTQNPLDFAL
                     TGPGFFTVMNPDGHLLYTRDGSFNLDADGRLVTKDGLPVMGHNGQIFIDGKNMVVDQE
                     GNITVDGKTVDQLAVVRVKDETQMVKVGHSLFKSSQATPVDKWPDNILVQQGFLEQSN
                     VDAIEEMVHMIDVTRSYEMNSRVVSTYDNIMGQAASEIGTLRV"
     gene            987..>1773
                     /gene="flgG"
                     /locus_tag="VFR02_03960"
     CDS             987..>1773
                     /gene="flgG"
                     /locus_tag="VFR02_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HET9869640.1"
                     /translation="MQSLYTAATGMEAQQMNIDTIANNLANVSTTGFKKQRVDFQDLL
                     YLNLKPAGTPTSSETKTPAPIEIGEGVKPVATDRIFTQGSVQNTNNPLDLMISGNNGF
                     FKVTLPNGDTAYTRDGTFHQDGDGNIVTSNGYYLDPQVQIPQDTSTITINPVGQILVS
                     EAGSTAQNQIGQLEISNFVNPGGLQSIGNNLFIATPASGEGIDGTPGSQGFGNITQGS
                     LEISNVDVVSEMVNLITAQRAYEMNAKAVSTADNMLATIAQLKQ"
BASE COUNT          398 a          563 c          533 g          279 t
ORIGIN      
        1 caagtaacgg aggaaccgac atgcccagtg gcctttacat cgcggcgtca gggatgaacg
       61 ccaccatgac ccaacaggac gtcctcgcca acaacctggc gaacgtcaac acggtggggt
      121 tcaagggcaa ccggaccgtg gacgcggctt ttcccaccta cctgatcgcc cggctcaatg
      181 accagcggct caagaccccg gacggggacc tggacctccg ccccaccatc ggactgatgg
      241 gagggggcgt catccccgcc gccaccccgg tggactacag ccagggctcg cgggtgcaga
      301 cccagaaccc cctggatttc gccttgaccg ggccgggctt ctttacggtc atgaacccgg
      361 acggccattt gctttacacc cgggacggca gcttcaacct ggatgccgac ggaaggttgg
      421 tgacgaagga cggcctgccg gtcatgggcc acaacggcca gatcttcatt gacggcaaga
      481 acatggtggt ggaccaagag ggcaacatca cggtggacgg aaagacggtc gaccaattgg
      541 ccgtggtccg ggtcaaggac gagacccaga tggtcaaggt ggggcattcg ctgttcaaga
      601 gctcccaggc cacgccggtg gacaagtggc ccgacaatat ccttgtgcaa caaggctttt
      661 tggaacaatc gaacgtggac gccatcgagg agatggtgca catgatcgac gtgacccggt
      721 cctacgaaat gaactcccgg gtggtctcga cctatgataa tatcatgggc caagcggctt
      781 ctgagatcgg caccctccgg gtctaacggg gcgccgtgtt tttgtggttg gagtggttgg
      841 ataggtaggg ttggtcgagc tggacaaaga cgaacggccg gactgcgaat gcggaggccg
      901 ttttaccgcc gaacgatcga ggcggtaaaa aaaaccgagc ccgtccgggc tgtgtccgga
      961 ggggcgcggg aatccgggag gcatttatgc agtcactgta cacggcggcc acgggcatgg
     1021 aagcccagca gatgaacatc gacaccatcg ccaacaatct ggccaacgtc agcaccacgg
     1081 ggttcaagaa acagcgggtc gatttccagg acctgcttta cctcaacctc aagcccgccg
     1141 gcaccccgac ttcctccgag accaagacgc cggcccccat cgagatcggc gagggcgtca
     1201 agcccgtggc caccgaccgc atcttcaccc agggaagcgt ccagaacacc aacaacccgc
     1261 tggacctgat gatctcgggc aacaacggct tcttcaaggt caccctgccc aacggcgaca
     1321 ccgcctatac ccgcgacggg accttccacc aggacggcga cgggaacatc gtcaccagca
     1381 acgggtacta cctggatccg caggtccaga tcccccagga caccagcacc atcaccatca
     1441 acccggtggg gcagatcctg gtgtcggaag cgggttccac ggcccagaac cagatcggcc
     1501 agttggagat cagcaacttc gtcaatccgg gcggcctgca atccatcggc aacaacctct
     1561 tcatcgccac cccggcttcc ggggagggca ttgacggcac cccgggaagc cagggtttcg
     1621 gcaacatcac ccagggttcg ttggagatct ccaacgtcga cgtcgtgtcc gagatggtga
     1681 acctgatcac ggcccagcgg gcctatgaga tgaacgccaa ggccgtgtcg acggccgaca
     1741 acatgctggc gaccatcgcc cagctcaagc agc
//
LOCUS       DASTJJ010000315         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_534555, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000315 DASTJJ010000000
VERSION     DASTJJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534555"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /locus_tag="VFR02_03965"
     CDS             <1..1892
                     /locus_tag="VFR02_03965"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9869641.1"
                     /translation="MLDQLQSVLTATLGKNSETRNRVLGDIKRFQAKENGDAEFAKAM
                     ADTGNVFLSMDAVFQTEQGSEGRSPDFADLKTTGVDLFGGGLSSFPKARSLIVSIPEL
                     QGKVEDSGLILYLPDLDGIYRYFPTVVGYQGHLLPNLSLQVSRYLLDDPNPVKLVNGR
                     YVEVAGRKLPVLDNGFAFINYCGPRGTIPSFSASDILYDRVPHDELKGTVALIGATAA
                     GLFDLRPTPFTKASPGVEINANILENIYSGNFIRLAPTLWKYLLILLMALVMWYLVPR
                     WTPTQGMVWFLLVFVAYIVVACLAFASGHVIINMTYPLVSLLLTYLILTTYKFRTEVK
                     HSRYMKQMFQSMVAPTVVEEILKLPEGIALGGEEKKLTVMFSDIRGFTTYSEKHGPSD
                     VVEILNEYLTQMTNMIFQTEGTLDKYIGDAIMAFWGAPTAQQDHAYRACSTALGMVDL
                     LENVLHPKWELEGKEKLEIGIGLNTGGMVVGFVGSESIKNYTLIGDAVNLGSRLEGTT
                     KEYKVSIIISETTYEEVKADMLCRELDLIQVKGKTKPIRIYELVDHRLKGAGEKELRV
                     RTFEAGLAHYRARRFDEASGEFKNCLQMDPKDGPAAIFLERCRILKQNPPPADWDGVF
                     IMKTK"
     gene            2020..2769
                     /locus_tag="VFR02_03970"
     CDS             2020..2769
                     /locus_tag="VFR02_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET9869642.1"
                     /translation="MTGIFPALFALFFFAVLSPASAAVSSQGTLISVSGKVEVRPQAG
                     GPGRSAKVGTLVGEGQRVVTGKDSGAILEFFDGSQVILKPGTDFWLSKLRKPSAKDKI
                     LHFKLLAGALLAQVTKLASASSSFEIEAGGVVCGVRGTQFSMEYDPAKDQVLLEVKEG
                     RVYARSRGWSQDFGAGERGRFHQGRWAGRPGGEGLPPSVPPPHLALDDMRSQFQRTVR
                     LGHDAVQTDPAVGGALNLHLVVGVPGQENLP"
     gene            2766..>3036
                     /locus_tag="VFR02_03975"
     CDS             2766..>3036
                     /locus_tag="VFR02_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869643.1"
                     /translation="MRGRAAFRACLLACLSTAPAGAGQLSLGTQADGWAANTLQPVRG
                     WEVMVPVSVDLDAAPGLGLYAQTAFAAAGYTDQTSLDLTAMTDSVV"
BASE COUNT          516 a         1036 c          953 g          531 t
ORIGIN      
        1 agatgctgga ccagctgcag tccgtgctga ccgccaccct gggcaagaat tcggagaccc
       61 ggaaccgggt gctgggggac atcaagcgtt tccaggccaa ggagaacggc gacgccgaat
      121 tcgccaaggc catggcggac accggcaacg tcttcttgtc gatggacgcg gtcttccaga
      181 ccgagcaggg gagcgagggc cgcagccccg acttcgccga cctcaagacc accggcgtgg
      241 acctcttcgg cggcggcctg tcctccttcc ccaaggcgcg ctccctcatc gtgtccatcc
      301 ccgagctgca gggcaaggtg gaggattcgg ggctcatcct ctacctgccc gacctggacg
      361 gcatctaccg ctatttcccc acggtggtgg gctaccaggg gcacctgctg cccaacctgt
      421 ccctgcaggt cagccgctac ctgttggacg accccaaccc cgtcaagctg gtgaacggcc
      481 gttacgtcga ggtggcgggc cgcaagctgc ccgtgctgga caacggcttc gccttcatca
      541 actattgcgg cccccgcggc accatcccca gcttctcggc cagcgacatc ctctatgacc
      601 gtgtgcccca cgacgaactg aaggggaccg tggcgctcat cggcgccacc gccgccgggc
      661 tcttcgacct gcgccccacc cccttcacca aggcctcgcc gggggtggag atcaacgcca
      721 acatcctgga gaacatctac agcggcaatt tcatccgcct ggcgcccacg ctctggaagt
      781 acctgctcat cctgctcatg gccctggtca tgtggtacct ggtgccccgt tggacgccca
      841 cccagggcat ggtgtggttc ctcctggtct tcgtggccta catcgtggtg gcctgcctgg
      901 ccttcgcctc gggccacgtg atcatcaaca tgacctatcc cctggtgtcg ctgctgctga
      961 cctacctgat cctgaccacc tataagttcc gcacggaggt caagcacagc cgctacatga
     1021 agcagatgtt ccagtccatg gtggccccca cggtggtgga ggagatcctg aagctgcccg
     1081 agggcatcgc gctgggcggc gaggaaaaga agctgaccgt catgttctcc gacatccggg
     1141 gcttcaccac ttattcggaa aagcacggcc cctcggacgt ggtcgagatc ctcaacgagt
     1201 acctgaccca gatgaccaac atgatcttcc agaccgaggg cacgctggac aagtacatcg
     1261 gcgacgccat catggccttc tggggcgcgc ccacggccca acaggaccac gcctaccggg
     1321 cctgcagcac ggccctgggg atggtggacc tgctggagaa cgtcctgcac cccaagtggg
     1381 aactggaagg caaggaaaag ctcgagatcg gcatcggcct caacacgggc ggcatggtgg
     1441 tggggttcgt ggggtccgag tccatcaaga actacacgct catcggcgac gcggtgaacc
     1501 tgggcagccg cctggaaggc accaccaagg aatacaaggt ctccatcatc atcagcgaga
     1561 cgacctacga ggaggtcaag gccgacatgc tctgccgcga actggacctc atccaggtca
     1621 agggcaagac caagcccatc cgcatctatg agctggtgga ccaccggctc aagggcgcgg
     1681 gcgagaagga gctgcgggtg cgcacctttg aggccggctt ggcccattac cgcgcgcgcc
     1741 gtttcgacga agcctccggc gagttcaaga actgcctgca gatggacccg aaggacggcc
     1801 cggcggccat cttcctggaa cgctgccgga tcctgaaaca gaacccgccg cccgcggatt
     1861 gggacggcgt cttcatcatg aagaccaagt agcttttccc ccgcgccctt gcctattccc
     1921 ttgggttaat tcttttccca attttcccgc tcccttggcc gccaatgttt ttcctttcag
     1981 gtaagatgac gtcattgagc cgccgtcaag cgaggcccca tgaccggaat ctttcccgcc
     2041 cttttcgccc tttttttctt cgccgtcctt tcccccgcct ccgccgcggt gtcctcccaa
     2101 ggcacgctga tctccgtctc gggcaaggtg gaggtgaggc cccaggccgg cggccccggc
     2161 cggtccgcca aggtggggac cctggtgggg gaaggccaac gggtcgtcac gggcaaggat
     2221 tcgggggcca tcctggagtt cttcgacggt tcccaggtca tcctcaagcc cggcacggat
     2281 ttttggctct ccaagctgcg caagccttcc gccaaggaca agatcctcca cttcaagctg
     2341 ctggccgggg cgctgttggc ccaggtcacc aagctggcct cggccagttc ttccttcgag
     2401 atcgaggcgg gcggggtggt ctgcggcgtc cggggcaccc aattttccat ggagtacgac
     2461 ccggccaagg accaggtcct tctggaggtg aaggaaggcc gggtctatgc gcggtcccgc
     2521 ggctggtccc aggatttcgg cgccggggaa cggggccgtt tccatcaggg ccgttgggcg
     2581 ggcaggcccg gcggggaagg gttgccgccg tccgtgccgc ccccgcacct ggccctggac
     2641 gacatgaggt cccagttcca gcggacggtg cgcctgggcc atgacgccgt gcagaccgac
     2701 ccggcggtgg gcggggcctt gaacctgcac ctggtggtcg gcgtgccggg ccaggagaac
     2761 ctgccatgag gggacgggcc gccttccgcg cctgcctctt ggcgtgcctt tcgaccgccc
     2821 cggcgggcgc cggccagttg agcctgggca cccaggcgga cggctgggcc gccaacaccc
     2881 tgcagcccgt ccggggctgg gaagtgatgg tgcccgtctc ggtggacctg gacgcggccc
     2941 cgggcctggg cctttacgcc cagacggcct tcgccgccgc cggatacacc gaccagacca
     3001 gcctggacct cacggccatg acggattcgg tggtgg
//
LOCUS       DASTJJ010000316         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_412525, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000316 DASTJJ010000000
VERSION     DASTJJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412525"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFR02_03980"
     CDS             <1..530
                     /locus_tag="VFR02_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869644.1"
                     /translation="DQFSPAAAPGTYQTGLLTNAAVSGTTGGNGLPVNVTGAPAAGST
                     VTIEVPTPTPTATPSPTRTPLPTPTPYGGDSFYISKNVFNPGTEPVSIHVVFQTSTVM
                     TDRNIPALRIYNSAGEFITKLSPLTDSSGRPVWDSWYRWDGTNLYHQPVASGLYIIYL
                     LEPYTAKEAKVLVLH"
     gene            657..1358
                     /locus_tag="VFR02_03985"
     CDS             657..1358
                     /locus_tag="VFR02_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869645.1"
                     /translation="MRALIPLSLALALLVPVASPAKRMPPPMLLPLDDGAYRFVVWND
                     FKKIKDNLYAGGHVVAYLKTTGEKRWEKYLYLVPINPKITRGDQAIYLKKMYLLKPNR
                     LVFQNERDEWFVLERRTGILLDGADFKKKSDDSSVYYGNDDRVEPILQGTYQVEASPS
                     VTYGHQTLKATDIPTGRVLWEKRVPVPRVGPQERSRILFLSLQGDRLRVSFEDGSSCL
                     MDATTGRILGTSRLK"
     gene            1522..3336
                     /locus_tag="VFR02_03990"
     CDS             1522..3336
                     /locus_tag="VFR02_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9869646.1"
                     /translation="MPVLAADWVDQVLPFSELVRILNFYLDLECWIISLSRPSPWMPI
                     RYHQRDPWPVELDDLFGRREARVLYNRTQIDRAVRGKKTVCAPFDGFHSFFIPILRGG
                     RPRGVIQCGPFLRREPTRRDLLEGWRAMTGGDVPRFDLDFSRYVRAVLETPVVEGPVF
                     RALRDLLLLHAQAASGEGGAAAMERACRTAERMKAEVFARHLPNRFWVEKAVKNNRLY
                     PPDWWVEKARADQWRREEQGLDREPSLVMAVMPDGEAQGSPDDLQTLLDHHRFQRRFF
                     EMAKNLPGTLAGPLEDYGMVFFTSPAPGANEVQAKLEILDRVDKLSQAARKELGIRIL
                     VGVGRCLGEERNLSRVYREAVLALEFCRPLSRPILFYEDLHHNPSLPKPPLFYEMSRK
                     LVEVYRTGAVRQVEPIRSRYIEQVLAHAAGRPETVRLHFLFVCGQVVDALTRKKPQQE
                     SELAGLLASLEKKLRETGDVQDLTAVFRDSLRRLLDLVLKPGTAPRSDRAAAARAFLD
                     KNFAEDLKLGEVARRHGFSTSVFAREFKRETGLGFSTYLKRVRVAHARRLLVSTQMPI
                     AQVSQDSGFNNLHYFFDVYKRETGQTPGESRSNNARSK"
BASE COUNT          648 a         1259 c         1120 g          659 t
ORIGIN      
        1 ccgaccagtt ctcgcccgcg gccgcgcccg gcacctacca gacgggtctc ttgaccaacg
       61 ccgcggtctc ggggaccacg ggcggcaatg ggttgccggt caacgtgacg ggggccccgg
      121 ccgcgggatc gacggtgacc atcgaggtcc ccacccccac gcccaccgcc acgcccagcc
      181 cgacgcgaac gcccctgccc acccccacgc cctacggggg cgattccttt tacatctcga
      241 agaacgtctt caacccgggc accgagccgg tgtccatcca cgtggtcttc cagaccagca
      301 cggtgatgac ggaccgcaac atcccggcgc tgcggatcta taattcggcg ggcgagttca
      361 tcaccaaact ctcgccgctc accgacagct cgggccggcc cgtgtgggat tcctggtacc
      421 ggtgggacgg gaccaacctg taccatcagc cggtggccag cggcctctac atcatctacc
      481 tgctggaacc ctacacggcc aaggaagcca aggtcctggt gctgcattag gcccggtcct
      541 tcggtttgag cgtgaagacc gacccgcgga aatcccccga ttcttggccc gaagttggtg
      601 aagcggggtc ccgccaccgc tagaatgggg catcccaacg ttggagcccc gaacgcatgc
      661 gagccctcat ccccctgtcg ctggccctgg ccctgctggt ccccgtcgct tcgcccgcca
      721 aacgcatgcc cccgcccatg ttgttgccgc tggatgacgg ggcctaccgg ttcgtggtct
      781 ggaacgactt caagaagatc aaggacaacc tctacgcggg cgggcacgtc gtggcctatc
      841 tcaagaccac cggcgagaag cggtgggaaa agtacctcta cctggtgccg atcaacccca
      901 agatcacccg gggggaccag gccatctacc tgaagaagat gtacctgctc aaacccaacc
      961 ggttggtgtt tcagaacgag cgggacgaat ggttcgtctt ggaacggcgc accgggatcc
     1021 tcttggacgg ggccgatttc aagaaaaaat cggacgattc ctcggtgtac tacggcaacg
     1081 acgaccgggt ggagcccatc ctgcagggca cctatcaggt ggaggcgtcc ccttcggtca
     1141 cctacggcca ccagaccctg aaggccaccg acatccccac gggccgggtg ctctgggaaa
     1201 aacgcgtgcc cgtgccccgg gtggggccgc aggaacgcag ccgcatcctg ttcctgtccc
     1261 tgcaggggga ccggttgcgg gtgagcttcg aggacggctc ctcctgcctg atggacgcca
     1321 ccacggggcg catcctggga accagccggc tcaagtagcg ccgaattatc ggtctttggg
     1381 aaaaagtttt ccccggtccc gtccgaaaaa acaaagcgcc gcttcccccg gtttgcacga
     1441 gaaagcgctt tggccccctc cgattcacct tgcatgttgg cttaaaaaag gtccaatggg
     1501 gaaaagtccc tggagggtcc catgccggtg ctggccgccg attgggtgga tcaagtcctg
     1561 cccttttccg aattggtccg catcctgaat ttttacctgg acctggaatg ttggatcatc
     1621 tccctcagcc gcccttcccc ctggatgccg atccgatacc accaacgcga tccctggccg
     1681 gtggagctgg acgacctttt cggccgccgg gaagcgcgcg tcctctataa ccgcacgcag
     1741 atcgaccgcg cggtccgggg taaaaagacc gtctgcgcgc ccttcgacgg attccacagt
     1801 tttttcatcc ccatcctgcg cgggggccgg ccccgggggg tgatccaatg cgggcccttc
     1861 ctcaggcggg agcccacccg gcgggacctg ctggaaggct ggcgcgccat gacgggcggc
     1921 gacgtgccgc gcttcgacct ggatttttcg cgctatgtgc gcgcggtctt ggagaccccg
     1981 gtggtggagg ggcccgtctt ccgcgccttg cgggaccttc tgttgctcca tgcccaagcg
     2041 gcatccggcg agggaggggc cgcggctatg gagcgggcct gccggaccgc cgaacgcatg
     2101 aaggccgagg tcttcgcccg gcatttaccg aaccgttttt gggtcgaaaa agccgtcaag
     2161 aacaaccgcc tctatccccc cgactggtgg gtcgaaaagg cccgcgcgga ccagtggcgc
     2221 cgggaggagc agggcctgga ccgggaacct tccctggtga tggccgtcat gcccgacggg
     2281 gaggcccagg gttcgccgga cgacctccag accctgttgg accaccaccg gttccagcgc
     2341 cgttttttcg agatggccaa aaacctgccc gggacgctgg cgggccccct ggaagattat
     2401 gggatggtct tcttcacctc ccccgcgccc ggggccaatg aggtccaggc caagctggag
     2461 atcctggacc gggtggacaa actgtcccag gccgcccgca aggaactggg catccgcatc
     2521 ctggtcggcg tgggacgatg cctgggcgag gagcgcaatt tatcccgggt ctaccgtgag
     2581 gcggtcctgg ccctggaatt ctgccggccc ctgtcccggc ccatcctttt ttacgaggat
     2641 ctccaccaca atcccagcct gcccaagccg cccctgttct atgagatgtc ccgcaagctg
     2701 gtggaggtct accgcacggg ggccgtgcgg caggtcgagc ccatccggag ccgctacatc
     2761 gagcaggtcc tggcccacgc ggcggggcgt cccgagacgg tgaggctcca cttcctgttc
     2821 gtctgcggcc aggtggtcga cgccttgacg cgcaagaagc cccagcagga aagcgagctc
     2881 gccggcctgt tggcgtcctt ggaaaaaaag ctccgggaga cgggggatgt gcaggacctg
     2941 acggccgttt tccgggattc gctccggcgc ctgttggacc tggtgctgaa gccggggacc
     3001 gcgccccgct ccgaccgggc cgccgccgcg cgagccttcc tggacaagaa tttcgccgag
     3061 gacctcaagc tgggggaggt ggcgcgccgg cacggttttt ccacgtcggt cttcgcgcgc
     3121 gaattcaagc gggaaacagg actgggcttc tcgacctacc tgaaacgggt ccgcgtggcc
     3181 cacgcccggc ggctgctggt ctccacccag atgcccatcg cccaggtcag ccaggattcg
     3241 gggttcaaca acctccacta ttttttcgac gtctacaaac gggagacggg acaaactccg
     3301 ggcgaatccc ggtccaataa cgcccggtca aaatagtctg gtttaatctt aaagctgccc
     3361 gtcctccaac acttcgcatc gttttccttt tgcccatcca tcctccgttt tttccgaacg
     3421 gattcaaccg aaatcctgaa ccggcgccct ccttccaaag ctaacctttt cccgtaatca
     3481 aaagatcaag accggcgccc aaactgcggg cccggtccgg ccgctgcaac ggcccaggaa
     3541 ccgcgaaaac gcgcatgact catatcgttt ttgccgcatc agccgcttcc gcccggcctt
     3601 ggggtttccc ccctgagggg gcacggaggg cctcccgcgc caccggcggg gtcctgtgga
     3661 ccctcctatg ctttttgttc ctcgcc
//
LOCUS       DASTJJ010000317         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1757544, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000317 DASTJJ010000000
VERSION     DASTJJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757544"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFR02_03995"
     CDS             <1..1005
                     /locus_tag="VFR02_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869647.1"
                     /translation="GSGALSIDSNTPVNYDPAVDSIYSVLEAVNANNSNVTALFNYNA
                     QQVLIFSNSPINMTDTNGTNGFMQGTQLNEILMSSIRMNNGFAPTDPQIDPATRMNTT
                     PANLGIAAPVPVPYTAGNSAAFRVTPSLTGSFTINGVQTDFTNQPPALNANGATPYTL
                     TQIANAISAGGNPITFPIGASFNIPSQQFILTLGPNSGTPPGSALSPIQIQDLTGNFS
                     VFTGLNASVAASNLASNLTSRVDADAQNADTLYAQANDALTQLNTAQAQVGTLSTGTG
                     TGPGNAGTPVAFMEEQAMQALVAYNASLEMLQIQNQMFADLLSVVAPPPPTGSSPFVL
                     "
BASE COUNT          219 a          394 c          280 g          161 t
ORIGIN      
        1 ggctcgggcg ccttgagcat cgattccaac accccggtca attacgaccc ggcggtggac
       61 agcatttaca gcgtgctgga agccgtcaac gccaacaaca gcaacgtgac ggccctgttc
      121 aactacaacg cccaacaggt gctgatcttc tccaactccc ccatcaacat gaccgacacc
      181 aacgggacca acgggttcat gcagggcacc cagctcaacg agatcctgat gagttccatc
      241 cgcatgaaca acgggttcgc ccccaccgac ccccagatcg acccggccac ccgcatgaac
      301 accacgccgg ccaacctggg catcgcggcc ccggtgcccg tgccctacac ggcgggcaat
      361 tcggcggcct tccgcgtgac cccgtccttg acgggttcct tcacgatcaa cggcgtccaa
      421 acggatttca ccaaccagcc cccggccttg aacgccaacg gggccacgcc ctataccctg
      481 acccagatcg ccaacgccat ttcggcgggc ggaaacccca tcaccttccc catcggcgcc
      541 agcttcaaca tccccagcca gcagttcatc ctcaccctgg ggcccaattc ggggacccca
      601 ccggggtccg ccttgagccc gatccagatc caggacctga cggggaactt ctcggtcttt
      661 acggggctca acgccagcgt ggcggcctcc aatctggcct ccaacctgac gtcccgggtg
      721 gacgccgacg cccagaacgc cgacaccctg tacgcgcagg ccaacgacgc cctgacccaa
      781 ttgaacaccg cccaggccca ggtggggacc ctttccaccg gtacggggac gggcccgggc
      841 aacgccggca ccccggtggc gttcatggaa gagcaggcca tgcaggcctt ggtcgcctac
      901 aacgcctcgt tggagatgct gcagatccag aaccagatgt tcgccgatct cttgagcgtg
      961 gtggccccgc ccccgcccac gggatcgtcg ccttttgtgc tgtaagaacg ggcaaggaat
     1021 gaaacggtga aaagcatcac ggaactcctg aact
//
LOCUS       DASTJJ010000318         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1757627, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000318 DASTJJ010000000
VERSION     DASTJJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757627"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1357
                     /locus_tag="VFR02_04000"
     CDS             <1..>1357
                     /locus_tag="VFR02_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HET9869648.1"
                     /translation="RRMMEFMIQYSLSLDELRRFEANYPGSPLACATLFQLGSRESQT
                     GDAPDAIQDLNSFLQQCPQDPSAPSAQLILQSLRDQQQGKTWRLAVLAPMTGRFKAFG
                     QSFVNGVNLAFDQAGNGHKMTLAAVQDTAGDPLRAVKLFQQMAQTGGVDAVLGPVVKS
                     EIDAVAPLANQQGIPLLAATYPVDGLSGIGPYVMGDSMTNEMQGRAMARFAVQKLGLK
                     RFGMLYPDDGYGQTLSRAFAQTVEALGATVTAQDAYTPNSTDFQKQIADLGGQNPESA
                     KENTRENQRRVGELDYDLGKDIQKFLFKAQQADPTSATVALVPLGEALANTNCPSIRQ
                     DVNDTLRTALQGAKGYTLRGADLVQQSLTRLPVEFRGTTLPVAADQWTDVATDLQSSV
                     IVTGRILGTNPPDDATLPTWNYSLELEAFQTDPKTGQVTKLAWDKLPLSVYKPPSFLP
                     A"
BASE COUNT          245 a          507 c          415 g          190 t
ORIGIN      
        1 gccgccgcat gatggagttc atgatccagt actcgctctc gctggacgaa ctcaggcgct
       61 tcgaggccaa ctatcccgga tcgcccctgg cctgcgccac cctgttccag ttgggcagcc
      121 gggaatccca aacgggcgac gccccggacg ccatccagga cctcaacagc ttcctccagc
      181 aatgccccca ggacccctcc gccccctcgg cccagttgat cctccagtcc ctccgggacc
      241 agcagcaggg caagacctgg cggctggccg tgttggcgcc catgaccggc cgcttcaagg
      301 cctttgggca atccttcgtc aacggggtca acctggcctt cgaccaggcg gggaacgggc
      361 acaagatgac cctggcggcg gtccaggaca cggcggggga cccgctgcgc gcggtcaaac
      421 tgttccaaca gatggcccag acgggcggcg tggacgccgt cctgggcccg gtggtcaagt
      481 ccgagatcga cgcggtggcg cccctggcca accaacaggg catccccctc ttggcggcca
      541 cctatccggt ggacggcctg tcgggcatcg gcccctacgt gatgggcgac agcatgacca
      601 acgagatgca ggggcgggcc atggcccgct tcgccgtcca gaagctgggc ctcaagcgct
      661 tcggcatgct ctatccggac gacggctacg gccagaccct gtcccgggcc ttcgcccaga
      721 ccgtggaggc cctgggggcc accgtgaccg cgcaggacgc ctacacgccc aattccaccg
      781 atttccagaa acagatcgcc gacctggggg ggcagaaccc cgagtccgcc aaggagaaca
      841 cccgcgagaa ccagcgccgg gtgggggaac tggattacga cctgggcaag gacatccaga
      901 aattcctctt caaggcccaa caggccgacc ccaccagcgc cactgtggcc ctggtgcccc
      961 tgggggaggc cttggccaac accaactgcc cctccatccg ccaggacgtc aacgacaccc
     1021 tgcggaccgc cctgcagggc gccaagggct ataccctgcg cggcgccgac ctggtgcagc
     1081 aatccctgac ccgcctgccc gtggagttcc gcggcaccac gctgccggtg gcggcggacc
     1141 aatggaccga cgtggccacg gacctgcaga gctccgtcat cgtgacgggc cgcatcctgg
     1201 ggaccaaccc gccggacgac gccaccctgc ccacctggaa ctactcgttg gaactggagg
     1261 ccttccagac cgaccccaag accggccagg tcaccaagct ggcctgggac aagctgccct
     1321 tgagcgttta taagccgccg tccttcttgc ccgccca
//
LOCUS       DASTJJ010000319         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1147196, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000319 DASTJJ010000000
VERSION     DASTJJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147196"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..>1011
                     /locus_tag="VFR02_04005"
     CDS             54..>1011
                     /locus_tag="VFR02_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET9869649.1"
                     /translation="MAIDTATRLSAVDKYFKITERGSNYSREIRGGFATFFAMSYIVV
                     LNPLILGGADSSGAVLGPARVAAMTALVAGLLTIIMGAWAKHPFALATGLGVNAFVAV
                     TVASHPGLTWPDMMGLVVLSGVTMFVLVLTGFRTAVFNAVPAGLKTAIVVGIGLFIAL
                     IGLVNAGFVRRIPDVAGTTVPVGLGFDGKLLGWPTLVFVVGLVLTIALVVRKVKGAIL
                     IGIIVSTVLANILQAVFNIGPSFDGKTANPEGWSLVVPHFSGLTPPDLGLFGQANLFG
                     SFANLGSLAALLLAFSILLSIFFDAMGTMVGLANEAGTVDKDG"
BASE COUNT          133 a          340 c          319 g          219 t
ORIGIN      
        1 gtattttctc gactggacta tccccctttt ctctttgccc cggagggcgc caaatggcaa
       61 tcgacacggc tacccgcctg tctgcggtag acaagtattt caagatcacc gagcgcggct
      121 cgaactattc gcgcgagatc cgcggtggct tcgcgacgtt cttcgccatg agctacatcg
      181 tggtcctcaa cccgctgatc ctgggcgggg cggattcctc cggcgcagtg ctgggcccgg
      241 cccgcgtggc ggccatgacc gcactggtgg ccgggctgct gaccatcatc atgggcgcct
      301 gggcgaagca cccgttcgcg ctggccaccg gcctgggcgt gaatgcgttc gtggccgtca
      361 ctgttgcctc ccatcccggc ctgacgtggc cggacatgat gggcctggtg gtgctctccg
      421 gcgtcaccat gttcgttctg gtcctcaccg gtttccggac cgccgtgttc aatgcggtgc
      481 cagccgggct gaagacggcc attgtggtgg ggatcgggct gttcattgcc ctgatcggcc
      541 tggtcaacgc cggctttgtg cgccgcatcc ccgacgtcgc cggcaccacc gtccccgtgg
      601 gtctgggctt cgacggcaag ctgctcggct ggccgaccct ggtctttgtg gtgggcctgg
      661 tgctgaccat cgcactggtg gtccgcaagg tcaagggcgc catcctgatc ggcatcatcg
      721 tttccaccgt ccttgccaac atcctgcagg ccgtcttcaa catcggtccc agcttcgacg
      781 gcaagactgc caaccccgag ggctggtccc tcgtggtgcc gcatttctcc gggctgaccc
      841 cgccggacct gggcctgttt gggcaggcca acctgtttgg ttcgttcgcc aacctgggct
      901 ccttggcggc cctgctgctg gcgttcagca ttctgctgag catcttcttc gacgccatgg
      961 gcaccatggt gggcctggcc aacgaggccg gcaccgtgga taaggacggc a
//
LOCUS       DASTJJ010000320         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_657459, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000320 DASTJJ010000000
VERSION     DASTJJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657459"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /locus_tag="VFR02_04010"
     CDS             complement(<1..2009)
                     /locus_tag="VFR02_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9869650.1"
                     /translation="MPFPVASRTPADYAGRMGSLLGWGLAALALILRLVQVQADWGRQ
                     QALFPAPVRGDFRAWVAEAPEVLYPREPGGHADGRAGEDYAPEVAEVRAACVLLSLDG
                     KPLAPVKARVTFTLSPRDPQPRLAYGGTVEFYGRVGPPSPALNPGQFDEARFLRDRGM
                     AYVGSADAGAWRPIPGAPRRGFFLARWAAGLKEGLEARVDALWPYPESALMEGVLLGD
                     RTGLPQDLVESFIVTGTVHILAVSGLITAFVAGALFLCFRALRAPRRAAAGLTLIGLV
                     FFVLVTGAHPPVLRAGIFAGLALTALLAGRRVWPGTLLLATAFLVALLNPFALEDFSF
                     QISFLATAGLMVMGPALLEKCSFLGKPLALVAASTLAAQLSVWALLLAAFHLLALYSV
                     PANLVVVPLVLFSVAGGLAALAGAFVHPLLGKLLAAGVFPALHLLMALAGWMARWPLA
                     QFPVASPPGAWLACFHAWLLWTFFAFWPRMRPEKPSADFLAKRAFFRRAKRAAVGGGV
                     FFLGRSAALGALDHWAQGPSLRVTFLAVGHGNAAVVRAPGGGLLVVDGGKETRGPDRY
                     LPLVSYLRALGARKVDAALDTHPDEDHVGGLVNLASAYPLGRAYEGEGATASTAVYRA
                     FHAELAARGDPLVPLEEGCALPEAAPAQALVLHPPRDFHPPEHAD"
     gene            complement(2049..2513)
                     /locus_tag="VFR02_04015"
     CDS             complement(2049..2513)
                     /locus_tag="VFR02_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869651.1"
                     /translation="MARRSLVLGAVLFLAGCGQPPPVGIDLLEAKKIADQTALDYFDA
                     NAKDLYPRLDARFHSQVHDPADLARVIRHIRFLYGKPVAYDYKAATRGQHEGPDGITP
                     YVDLWYVLRTDKYPKGDHYLKIEVVQAQGATFPDLGGFGVLDFPDGLPAYLQ"
     gene            complement(2516..>2992)
                     /locus_tag="VFR02_04020"
     CDS             complement(2516..>2992)
                     /locus_tag="VFR02_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HET9869652.1"
                     /translation="LMTEAKGPGRIAFSRDGVGHVFALHLEKGQSIDVREHQFLAATD
                     NVSYGFTRVKGVANMLLGGTGFFIDQFACESGKEGVLWLHGYGNVFEVDLKAGEQIDI
                     EPGGWIYKDQSVKMETLFQRLSTGLFGGAGQLFWNRFTGPGRVALQSMYLHMPTED"
BASE COUNT          515 a         1060 c         1054 g          363 t
ORIGIN      
        1 ttgtcggcgt gttcgggggg atggaaatcc cggggcgggt gcaggacgag cgcttgcgcg
       61 ggcgccgcct cgggcaaggc acacccctcc tccagcggga ccagcggatc cccccgggcc
      121 gcgagttcgg cgtggaaggc ccgatagacc gccgtggagg ccgtggcgcc ctccccctcg
      181 taggcccggc ccaggggata ggccgaggcc aggttcacca ggccgcccac gtggtcctcg
      241 tcgggatggg tgtccagcgc ggcgtcgacc ttccgcgccc ccagggcgcg caggtaggac
      301 acgaggggca ggtagcggtc cggcccgcgg gtctccttgc cgccgtccac caccagcagc
      361 ccgcccccgg gcgcgcgcac caccgccgcg ttgccgtggc ccacggccag gaaggtcacc
      421 cgcagcgaag gcccctgggc ccaatggtcc agggccccca gcgccgccga ccggcccagg
      481 aagaagaccc cgccccccac cgccgccctt ttcgcgcgcc ggaaaaaagc ccgcttggcc
      541 aggaagtccg cggaaggttt ttccggcctc atcctcggcc agaaggcgaa gaaggtccaa
      601 agcagccagg cgtggaaaca ggccagccag gcccccggcg gcgaagccac cgggaactgg
      661 gccaggggcc agcgggccat ccaaccggcg agcgccatca aaagatgcag ggcgggaaag
      721 acgcccgcgg ccaaaagttt ccccaacaag ggatggacga aggcccccgc cagggcggcc
      781 aacccccccg ccacgctgaa gagcaccagg ggcaccacca ccaggttggc gggcaccgaa
      841 tagagcgcca gcaggtggaa ggccgccagc aagagcgccc acaccgacaa ttgggcggcc
      901 agggtggagg cggcgaccag ggccaggggc ttccccaaga aggaacattt ctccaacagg
      961 gcgggcccca tcaccatgag ccccgcggtg gccaggaagg agatctggaa ggaaaagtcc
     1021 tccaacgcga aggggttcag gagggccacc aggaaggccg tcgccaagag cagggtgccg
     1081 ggccagaccc gccgcccggc cagcagcgcc gtgagcgcca gccccgcgaa gatgccggcc
     1141 ctcagcacgg gcggatgggc gccggtcacc agcacgaaaa agaccagccc gatcaaggtg
     1201 agcccggccg ccgcgcgccg gggcgcccgc agcgcccgga agcaaaggaa gagcgcgccc
     1261 gccacgaagg ccgtgatgag gcccgacacc gccaggatgt gcactgtgcc cgtgacgatg
     1321 aagctctcca ccaggtcctg gggcaggccc gtgcggtcgc ccaggagcac gccctccatc
     1381 agggcgctct ccggataggg ccagagggcg tcgacgcgcg cttccaaccc ttccttcagc
     1441 ccggcggccc aacgcgccag gaagaagccg cgccgggggg cgccggggat gggccgccaa
     1501 gcccccgcgt cggccgaccc cacgtacgcc atcccccggt cccgcaggaa gcgcgcctca
     1561 tcgaattggc cggggttgag agccggggag ggcgggccga cccggccgta gaattccacc
     1621 gtcccgccgt aggccaggcg cggctggggg tcccgcgggg aaagggtgaa ggtgacgcgc
     1681 gccttcacgg gcgccagggg cttgccgtcg agggaaagca gcacgcaggc cgcgcggact
     1741 tcggccacct ccggggcgta atcctccccc gcccggccgt ccgcatggcc gccgggttcc
     1801 ctgggataaa ggacctcggg cgcctccgcc acccaggcgc ggaaatcgcc ccggacgggc
     1861 gcgggaaaaa gggcctgctg gcgcccccag tccgcctgga cctggacgag gcgcaggatg
     1921 agcgccaagg ccgccaaccc ccacccgaga agggatccca tgcggccagc ataatccgcg
     1981 ggggtccggg acgccacggg gaaaggcacg gcaaggaccg gcctttattt ctgtctattt
     2041 tttgtctttt actggaggta ggccggcagc ccgtcgggga agtccagcac gccgaagccc
     2101 cccaggtccg ggaaggtggc gccctgggcc tggaccacct cgatcttcag gtagtggtcc
     2161 cccttgggat atttgtccgt gcgcaggacg taccagaggt ccacgtaggg cgtgatgccg
     2221 tcgggcccct cgtgctggcc ccgggtggcg gccttgtagt cgtaggccac cggcttgccg
     2281 tagaggaacc ggatgtggcg gatcacgcgg gccaggtccg ccgggtcgtg gacctggctg
     2341 tggaaccggg cgtccagccg gggatagagg tccttggcgt tggcgtcgaa ataatccagc
     2401 gccgtctggt cggcgatctt cttggcctcc agcaggtcga tcccgacggg cgggggctgc
     2461 ccgcaaccgg ccaggaacag gaccgccccc aggaccagcg accgccgcgc caacctcaat
     2521 cctcggtggg catgtgcagg tacatgctct gcagcgccac ccggcccggg cccgtgaagc
     2581 ggttccagaa gagctggccc gcaccgccga agaggccggt ggagagccgc tggaagaggg
     2641 tctccatctt gacgctctgg tccttgtaga tccagccgcc gggctcgatg tcgatctgtt
     2701 cgccggcctt caggtccacc tcgaacacgt tgccgtagcc gtgcagccac agcacgcctt
     2761 ccttgcccga ctcgcaggcg aactggtcga tgaagaagcc cgtgcccccc agcagcatgt
     2821 tggccacgcc cttgacccgg gtgaagccgt agctgacgtt gtcggtggcg gccaggaact
     2881 ggtgttcccg cacgtcgatg gactggccct tttccaggtg gagggcgaag acgtggccca
     2941 cgccgtcccg gctgaaggcg atgcggcccg ggcccttggc ctcggtcatc ag
//
LOCUS       DASTJJ010000321         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_291214, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000321 DASTJJ010000000
VERSION     DASTJJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291214"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..423)
                     /locus_tag="VFR02_04025"
                     /pseudo
     CDS             complement(1..423)
                     /locus_tag="VFR02_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(602..>3601)
                     /gene="rpoB"
                     /locus_tag="VFR02_04030"
     CDS             complement(602..>3601)
                     /gene="rpoB"
                     /locus_tag="VFR02_04030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9869653.1"
                     /translation="EVENCEVNEKKLQGKAAAADVANPETGEVVVEAGADFTGEVVAV
                     LKQLGVKQVKAVLSNPNDVSILSTLRKDTTRSIETALVEVYKKMRPGDPPTLDNAKSL
                     MENLFFNKKRYDLARVGRYKMNKKLGLKTALDQRTISKEDLVLIIRYLFDLCNGVGEK
                     DDIDHLGNRRVRTAGELLENKLRLGFTRMERAVRERMSIVDMDMNPMPSQLISTKPIT
                     AAVKEFFGTSQLSQFMDQTNPLAELTHKRRLSALGPGGLNRERAGFEVRDVHHTHYGR
                     VCPIETPEGPNIGLIASLSTYAQINEYGFVTTPLRRVEDGKVGRKIEYMPADDADKYI
                     IAQANTPLDDNNKFVASNNISARFKDDFPLVAPDEINYMDVSPRQLFSVATSLIPFLE
                     HDDANRALMGSNMQRQAVPLIKTEAPFVGTGMEYKAAYDSGVLVIAKRDGVVAGVSSD
                     EIIIFADEADETGSVDIYKLRKYKRSNQDTCINQRPIVRKGDKVKKGDVVADGPSTDQ
                     GELALGRNLLVAFMPWRGFNFEDAIIVSEKLVREDYFTSVHIEEFELEARDTKLGKEE
                     ITRDIPNVGDEALKDLDEDGIVRIGAEVMPGDILVGKVTPKGETELTPEEKLLRAIFG
                     EKAGDVRDASLRVPPGVEGKIIDVKVFKRKEKDEKMKGRDAAVLKAIDKEVTHKIRQL
                     EKENERHIGVLKAQYDEKAKDANKAEKSALQDKLEEETKRLNEVLANRVSDLEREKNN
                     EIEKVKKGDELPPGVIKLVKVYVAKKRKLSVGDKMAGRHGNKGVVSKIVPVEDMPYLP
                     NGRQVDIILNPLGVPSRMNVGQILETHLGWAAHEGGYHVASPVFDGAREEEIKRALKD
                     QGLPETGKIKLYDGMTGETFDQEVTVGYIYMLKLAHLVDDKIHARSIGPYSLITQQPL
                     GGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTVKSDDVQGRTKVYEAIVKGKNAPEP
                     GIPESFNVLVKELQGLCLDVKLMKNSN"
BASE COUNT          512 a         1152 c         1146 g          791 t
ORIGIN      
        1 gcccatcttg gccttcaggg acgcgccgta ttcctggcgc agcttctggt attcgtcctc
       61 gctcaagagc tggcgcactt ccaccggggc cttgcccggg tcggtgacca cgtaggattc
      121 gtagtagatc acccgttcca ggttgcgggt ggacatttcc agcaggtagc ccatgcggga
      181 cggcacgccc ttcaggtacc agatgtgcga caccaggcag gccagctcga tgtggcccat
      241 gcgctcacgg cgcaccttgg cctgggtgac ctccacgccg cagcggtcgc agatgacgtc
      301 cttgtacttg atgcgcttgt acttgccgca ggcgcattcc cagtccttgg tgggcccgaa
      361 gatcttctcg cagaacaagc cgttcttttc gggcttgaag gtgcggtagt tgatggtctc
      421 gggggaggcc aggccgatct tgatggcgtc gaaacgcacg gtctccttga tgagggtctc
      481 caaggtgtcg ttcttcacct tttcggccac caacaagggg gcctccggag ccgccggcaa
      541 ggccgcttcg ctgccgtttt cgggggtcgt cggtttcagg ttctgggtca tggtcccctc
      601 cttaattcga gttcttcatg agtttgacat ccaagcagag cccttgcagt tccttgacca
      661 ggacgttgaa ggattccggg atgcccggtt cgggggcgtt cttgcccttg acgatggctt
      721 cgtacacctt ggtgcggccc tgcacgtcgt cggacttcac cgtcagcagc tcctgcaggg
      781 tgtaggccgc gccgtaggct tccagcgccc agacttccat ttcgccgaag cgctggccgc
      841 cgaactgggc cttgccgccc aggggctgct gggtgatgag cgaatagggg ccgatggacc
      901 gggcgtggat cttgtcgtcc accaggtggg ccagcttgag catgtagatg tagcccacgg
      961 tgacctcctg gtcgaaggtc tcgcccgtca tgccgtcgta cagcttgatc ttgcccgtct
     1021 cgggcaggcc ctggtccttc aaggccctct tgatctcctc ctcgcgggcg ccgtcgaaca
     1081 ccggcgaagc cacgtggtag ccgccctcgt gggccgccca gcccaggtgg gtctccagga
     1141 tctggcccac gttcatgcgg gaaggcacgc ccagcgggtt caggatgatg tccacctgcc
     1201 ggccgttggg caggtagggc atgtcctcga ccggcacgat cttggacacc acgcccttgt
     1261 tgccgtggcg gcccgccatc ttgtcgccga ccgagagctt gcgcttcttg gccacgtaca
     1321 ccttgaccag cttgatcacg cccgggggca gctcatcgcc cttcttgacc ttctcgatct
     1381 cgttgttctt ctcgcgttcc aggtcgctga cccggttggc caacacctcg ttcaggcgct
     1441 tggtctcctc ttccagcttg tcctggaggg cggacttctc ggccttgttg gcgtccttgg
     1501 ccttttcgtc gtactgggcc ttcaggaccc cgatatgccg ctcgttctcc ttctccaact
     1561 ggcggatctt gtgggtgact tccttgtcga tggccttcag cacggccgcg tcgcggccct
     1621 tcatcttctc gtccttctcc ttgcgcttga agaccttcac gtcgatgatc ttgccctcca
     1681 cgccgggcgg caccctcaag gaggcgtccc gcacgtcgcc ggccttctcg ccgaagatgg
     1741 cccggagcag cttctcctcg ggcgtcagtt cggtctcacc cttgggggtc accttgccca
     1801 ccaggatgtc gcccggcatg acctcggcgc cgatgcgcac gatgccgtcc tcgtccaggt
     1861 ccttcagggc ctcgtcgccc acgttgggga tgtcccgggt gatctcttcc ttgcccagct
     1921 tggtgtcgcg ggcttccagc tcgaattcct cgatgtgcac cgaggtgaag tagtcctccc
     1981 gcaccagctt ctcggacacg atgatggcgt cctcgaagtt gaacccgcgc cagggcatga
     2041 aggccaccaa gaggttgcgg cccagggcca gctcgccctg gtcggtggag gggccgtcgg
     2101 ccaccacatc gcccttcttc accttgtcgc ccttgcggac gatcgggcgc tggttgatgc
     2161 aggtgtcctg gttggagcgc ttgtacttgc gcagtttata gatgtccacg gagcccgtct
     2221 cgtcggcctc gtcggcgaag atgatgatct cgtccgagga gacccccgcc accacgccgt
     2281 cgcgcttggc gatgaccagc acgcccgaat cgtaggcggc cttgtattcc atgccggtgc
     2341 ccacgaaggg ggcctcggtc ttgatcaagg gcacggcctg gcgctgcatg ttggagccca
     2401 tgagcgcgcg gttggcgtcg tcatgctcca ggaaggggat gagggacgtg gccacggaga
     2461 aaagctggcg cggcgacacg tccatatagt tgatctcgtc gggcgccacc agggggaaat
     2521 cgtccttgaa gcgcgccgag atgttgttgg aggccacgaa cttgttgttg tcgtccagcg
     2581 gcgtgttggc ctgggcgatg atgtacttgt ccgcgtcgtc ggcgggcatg tactcgatct
     2641 tgcggcccac cttgccgtcc tccacccggc gcaacggggt ggtcacgaag ccgtactcgt
     2701 tgatctgggc gtaggtcgac agcgaggcga tgaggccgat gttcggccct tccggcgtct
     2761 cgatggggca gacgcggcca tagtgggtat ggtgcacgtc gcgcacctcg aagccggcgc
     2821 gctcccggtt gaggccgccg ggaccgaggg ccgacaggcg gcgcttgtgg gtgagttcgg
     2881 ccaggggatt ggtctggtcc atgaactggg acagctggga ggtcccgaag aactccttca
     2941 cggcggccgt gatgggcttg gtggagatga gctggctggg catggggttc atgtccatgt
     3001 ccacgatgga catgcgctcg cggaccgcgc gttccatgcg ggtgaagccg aggcgcagct
     3061 tgttctcgag caattcgccc gcggtgcgca cccggcggtt gcccaggtgg tcgatgtcgt
     3121 ccttctcgcc caccccgttg cacaggtcga acaggtagcg gatgatcagc accaggtctt
     3181 ccttcgagat ggtgcgctgg tccaaggcgg tcttcaggcc cagctttttg ttcatcttat
     3241 aacggcccac ccgggccagg tcgtagcgct tcttgttgaa gaacaggttc tccatcaggc
     3301 tcttggcgtt gtccagcgtg ggcggatcgc ccggacgcat cttcttatag acctcgacca
     3361 acgcggtctc gatggaccgg gtggtgtcct tgcgcagggt gctcaggatg gacacgtcgt
     3421 tggggttgga aagcacggcc ttcacctgct tgacgcccaa ctgcttgagg accgccacca
     3481 cctcaccggt gaagtcggcc ccggcttcca ccaccacctc gccggtctcg gggttggcca
     3541 cgtcggcggc ggcggccttg ccctgcagct tcttctcgtt cacttcgcag ttctccacct
     3601 c
//
LOCUS       DASTJJ010000322         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_657911, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000322 DASTJJ010000000
VERSION     DASTJJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657911"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFR02_04035"
     CDS             complement(<1..430)
                     /locus_tag="VFR02_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9869654.1"
                     /translation="MKKTILAFCLALAPAVGAAPADLPRPQVAPWNGHKAAVSLTFDD
                     SDPSHLDVAIPELERRGLRGTFFLIANRTDRKAEWRKAYQAGQELGNHSLDHLHAAGL
                     APDEEKSQVVGAQTVLQNAFGVPFLTFAYPYTEITPGLLKW"
     gene            562..1620
                     /locus_tag="VFR02_04040"
     CDS             562..1620
                     /locus_tag="VFR02_04040"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869655.1"
                     /translation="MTPFEKAEHLRARSRFREAEKVYRQILRREKPGPAHRAEALLGL
                     ADVERIQGDFRASLSHYRAAEALLMGSDPDAAMDAKVGWALAARAMGRPKEALAVLQK
                     ALRFYRKDRDPAGLAFTHWALGGTLRIAGRMKEGLRELDQALALFKRLRDLDGVAYTC
                     CALGGLHRMLGGYAASGKYYREANRRMRALDDVFGTAYSYCGLGNVERMAGRFRSALP
                     YYCKAERLYGLIGDKVSYAYTLWSIGTAYKMLGRHYPEAHWAFYKADRLFQKTGDTRG
                     RTYALLGFAESEWLQGHQAKGDAYWKRAKKIADQSDFAWEALHVESLRGGKVRDLSKR
                     YRNAGSKFYPKTLPVNWP"
     gene            complement(1707..2393)
                     /locus_tag="VFR02_04045"
     CDS             complement(1707..2393)
                     /locus_tag="VFR02_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9869656.1"
                     /translation="MIFSEVEVQKHRCLVLDPPVVPPGAPALATLHGLGTNGEDLAAL
                     CQAFHLEGCRFVLPDAPLHLPGYPPGAYAWYDFQAPDRRQFVDSREYLFKVFERFTKD
                     PNLRPAPGKEKKPSPLVVMGFSQGGVMSMESGLNYRGKVAAIVSMSGYLPDPWETLTR
                     AEAPFDTPILLVHGTEDDVVPVEGSRKAHQALKEAGYRPVLKEFPMGHQITEASYKAV
                     REFLKDVLKI"
     gene            complement(2398..>3418)
                     /locus_tag="VFR02_04050"
     CDS             complement(2398..>3418)
                     /locus_tag="VFR02_04050"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET9869657.1"
                     /translation="SNTAFLMAGALLSNLLGTTGASLLLIRPFLHANRHRQDKTHLVV
                     FFIFIVSNGGAILPLGPPLYLGFLKQVPFFWNLNLVPAFGFWVLMLLVCFYFVDRRYC
                     ARDRGADQASPETLVFRLEGWKNAGWLLLIVGAILFSGYVVAPALKGPLGDEGAEDTS
                     KLFQMAVMGIIAWVSYQTTPAKIHEENQFHFWPIQELAILFFGIFGAMIPLLALLATQ
                     GQALPLNQPWQYFWAAGPISSILDNAPVYLNFCVLAASQQGIPGGRLDELAEQFPRFL
                     IAISCGASFMGALTYIGNGPNLLVKAVAEKAGVKMPSFGGYLAWSSAFLFPAFLIETL
                     VFFRS"
BASE COUNT          662 a         1080 c         1122 g          554 t
ORIGIN      
        1 cccacttcaa caatcccggc gtgatctcgg tatagggata agcgaaggtg aggaagggca
       61 ccccgaaggc gttctggagc accgtctggg ccccgaccac ctgggacttc tcctcgtccg
      121 gcgcgagccc cgcggcgtgg aggtggtcca ggctgtggtt gcccaactcc tggcccgctt
      181 ggtaggcctt gcgccactcg gccttgcggt ccgttcggtt ggcgatcaag aagaaggtcc
      241 cccgcagtcc gcgccgttcc agttcgggga tggccacatc caggtgggac gggtcgctgt
      301 cgtcgaaggt gagcgatacc gccgccttgt ggccgttcca gggggccacc tggggccggg
      361 gaaggtcggc gggggccgcc ccgaccgccg gggcgagggc caggcaaaag gccaggatgg
      421 tttttttcat gggaactcca ggttcatttt ccgggatccg cgcccggctc cgttaaaaac
      481 aacgccgggc atccggcttc gttataagac tacgccggac aagagggtat actatccccc
      541 actttcgatt caaggacccc catgacccca ttcgaaaagg ccgaacatct gcgcgcccgg
      601 tcccgcttcc gggaggctga aaaagtttac cgacaaatcc tgcgccggga aaaaccgggc
      661 cccgcccacc gggcggaggc cttgttgggg ctggcggacg tggagcgcat ccaaggcgat
      721 ttccgggcgt ccctttcgca ttatcgggcc gcggaggccc tcctgatggg ctcggacccc
      781 gacgccgcca tggacgccaa ggtcggctgg gccctggccg cccgggccat gggaaggccg
      841 aaagaagccc tggcggtgct gcaaaaagcc cttcgttttt accgcaagga ccgggacccc
      901 gcgggcctgg ctttcaccca ttgggccctg ggcgggacgt tgcgcatcgc cgggcgcatg
      961 aaggaaggcc tgcgggaact cgaccaagcc ctggcgctgt tcaaacgcct gagggatttg
     1021 gacggcgtcg cctacacctg ctgcgccctc ggcggcctgc accgcatgtt gggcggttat
     1081 gccgcctcgg gcaagtatta ccgggaagcc aaccgccgca tgcgggccct ggacgacgtc
     1141 ttcggcaccg cctattccta ctgcggcctg ggcaacgtcg agcgcatggc gggccgcttc
     1201 cgttccgccc ttccctacta ctgcaaggcc gagcgcctct acggcctcat cggcgacaag
     1261 gtcagctacg cctataccct ctggtccatc ggcacggcct acaagatgct gggccgccat
     1321 taccccgaag cccactgggc cttttacaag gcggaccgcc tcttccaaaa gaccggggac
     1381 acccgcggca ggacctatgc cctgctcggc ttcgccgaaa gcgaatggct gcagggacat
     1441 caagccaagg gcgacgccta ttggaaaagg gccaagaaga tcgccgacca aagcgacttc
     1501 gcctgggaag ccctgcacgt ggaatctctc aggggcggga aggtgaggga cctctcgaag
     1561 cgctaccgga atgccggatc gaagttctac cccaagacgc ttccggtcaa ctggccttag
     1621 agcttctaac aaaaatcaaa ataaaggcca ttttcaccgc cacgcccaag ggcgtgtgcg
     1681 gtgaaacagg tttttgttgg acgttcttag atcttcagga catccttcaa aaattcccgc
     1741 accgccttgt agctggcctc cgtgatctgg tggcccatgg ggaattcctt caggaccggg
     1801 cggtagccgg cttccttcag cgcttgatgg gccttgcggg aaccttccac cggcaccacg
     1861 tcgtcctcgg tgccgtgcac caacaggatg ggcgtatcaa aaggcgcctc ggcacgcgtg
     1921 agcgtttccc aggggtcggg caggtagccg ctcatggaaa cgatggcagc gaccttcccc
     1981 ctatagttca agcccgattc catcgacatc acgccgcctt gggaaaagcc catcaccacc
     2041 aggggggagg gcttcttctc tttcccgggc gcgggccgaa gattcgggtc cttcgtgaag
     2101 cgctcgaaga ccttgaagag gtattcccgg ctgtcgacga actggcgccg gtcgggcgcc
     2161 tggaaatcgt accaagcgta ggcgcccggc gggtaacccg gcaggtgcag gggcgcgtcg
     2221 ggcagtacga agcggcagcc ctccaaatgg aaggcctggc agagggcggc caggtcctcg
     2281 ccgttggtcc ccaggccgtg cagggtggcc agggccggcg cccccggcgg caccacgggg
     2341 ggatccagca ccaggcagcg gtgtttttgg acctcgacct cggagaagat catgggatca
     2401 ggaccggaag aagaccagcg tctcgatgag gaaggccggg aacaggaagg cggaggacca
     2461 ggccaggtag cccccgaagg aaggcatctt cacccccgcc ttttccgcca cggccttcac
     2521 cagcaggttc gggccgttgc cgatataggt gagcgccccc atgaaggaag ccccgcagga
     2581 gatggcgatg aggaaccggg ggaactgttc ggccagctca tccaggcgcc cacccgggat
     2641 cccctgctgg ctggcggcca gcacgcagaa gttcaggtag accggcgcgt tgtccaggat
     2701 gctgctgatg ggacccgccg cccaaaaata ctgccagggc tggttgaggg gcagggcctg
     2761 gccctgggtg gccaagaggg ccagcagggg gatcatggcg ccgaagatgc cgaagaagag
     2821 gatggccagt tcctggatgg gccaaaaatg gaactggttc tcctcgtgga tcttcgccgg
     2881 cgtggtctga taggagaccc aggcgatgat ccccatgacc gccatctgga aaagtttgga
     2941 ggtgtcttcc gccccctcgt cgcccaaggg gcccttcagg gcgggggcca ccacgtaacc
     3001 cgaaaagagg atggccccga cgatgagcaa aagccagccg gcgttcttcc agccttccag
     3061 gcggaagacc agggtctcgg gcgaggcctg gtccgcgccc cggtccctgg cgcagtagcg
     3121 ccggtccacg aagtaaaaac acaccaacag catcaagacc cagaagccga aggcgggcac
     3181 caagttcagg ttccaaaaga aaggcacctg cttgaggaag cccaggtaaa gcggcggccc
     3241 caggggcagg atggcgcccc cgttggagac gatgaagatg aagaacacca ccaggtgggt
     3301 cttgtcctgc cggtggcggt tggcgtgcag gaaggggcgg atgagcagca ggctcgcccc
     3361 cgtggtgccc aagaggttcg acaacagggc ccccgccatc aggaaggccg tgttggac
//
LOCUS       DASTJJ010000323         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1147773, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000323 DASTJJ010000000
VERSION     DASTJJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147773"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFR02_04055"
     CDS             complement(<1..1383)
                     /locus_tag="VFR02_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9869658.1"
                     /translation="MGEAGKTTYTCPMHPEVVRDRPGDCPKCGMHLEPLSVPAEGAPD
                     DGEWKDVNRRFWVGAVLTTPLIGWMTAELLAGHSRMSAVPPRWPSLGQALLSSLVVVG
                     CGWPLWVKAWRSLALRSPNMFTLIGLGVSAAYFYSLAAALAPGLFPPSFRMPDGSVPV
                     YFESSAAITVLVLLGQVLELRARSRTGAALQGLLGLAARSAHRVLPDGAEEEAALEKV
                     QAGDRLRVKPGEKVPVDGVVLEGRSSLDESMVTGEPLPVEKGPGDGVVGATVNGRGSF
                     LMRAEKVGEETLLSRIVSMVSQAKRSRAPVQKLADKVSGYFVPAVVGVACLAALAWGL
                     WGPDPKMANALLHAVAVLIIACPCALGLATPMSIMVAMGRGAAAGVLFKDAEAIEALR
                     RVDTLVLDKTGTLTEGKPRLASVVPARGMEGSEVLRLAASLERDSEHPLASAIVKEAE
                     GKGLVLEKVED"
     gene            complement(1388..1531)
                     /locus_tag="VFR02_04060"
     CDS             complement(1388..1531)
                     /locus_tag="VFR02_04060"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9869659.1"
                     /translation="MDREATMNEGEQEEKKGSEYTCPMHPEVKADAPGKCPKCGMLLQ
                     PRR"
BASE COUNT          257 a          593 c          467 g          235 t
ORIGIN      
        1 atcttccact ttttccagga ccaacccctt tccttcggcc tccttcacga tggcggaggc
       61 cagtgggtgc tcgctgtccc gctccaggga cgccgccaac cgcaggactt cggacccctc
      121 catcccccgg gccggcacca cggaggccaa ccggggtttc ccttcggtca gggtcccggt
      181 cttgtccaac accagcgtgt ccacgcggcg cagcgcctca atggcctcgg cgtccttgaa
      241 aagcaccccc gccgcggccc cccttcccat ggccaccatg atggacatcg gggtggccaa
      301 tcccagcgcg caggggcacg cgatgatgag caccgccacc gcgtgcagga gggcgttcgc
      361 catcttgggg tcgggccccc aaaggcccca agcgagggcc gccaaacacg ccacccccac
      421 cacggcgggg acgaaataac ccgacacctt gtcggccagc ttctggacgg gggcccggct
      481 ccttttggcc tgggacacca tggagacgat ccgggaaagc agggtctcct cccccacctt
      541 ctcggcccgc atcaggaagg acccgcggcc gttcaccgtg gcgcccacga ccccgtcccc
      601 gggacctttt tccacgggca agggttcgcc cgtgaccatg gactcatcca gggaactccg
      661 gccctccagg accacgccgt ccaccgggac cttttccccg ggcttgaccc tcaagcggtc
      721 ccccgcctgg accttctcca acgccgcctc ttcctcggcc ccatcgggaa ggacccggtg
      781 ggcggaccgg gcggcgaggc ccaaaagccc ctggagggcg gcgccggtcc ggctccgcgc
      841 cctcagttcc aggacctggc ccagcaggac cagcaccgtg atcgccgccg aggactcgaa
      901 ataaacgggc accgaaccgt ccggcatccg gaacgacggg gggaagagcc ccggcgccag
      961 ggcggcggcc aggctgtaaa agtaggcggc ggaaaccccc agcccgatca aggtgaacat
     1021 gttggggctg cgcaaggcca gggagcgcca ggccttgacc caaaggggcc atccacaccc
     1081 gaccacgacc agcgaggata aaagcgcctg gcccagggac ggccagcgcg gcgggaccgc
     1141 cgacatccgg gaatgccccg cgaggagttc ggcggtcatc cacccgatca ggggggtggt
     1201 caggacggcg cccacccaaa accgccggtt cacgtccttc cattccccgt cgtccggcgc
     1261 cccttccgcc ggaacggaaa ggggctccag gtgcatcccg catttggggc aatcccccgg
     1321 acggtcccgc accacttccg ggtgcatggg acaggtatag gtggtcttcc cggcctcccc
     1381 catggggcta ccgcctcggc tgcagcaaca tcccgcactt ggggcacttc cccggcgcgt
     1441 ccgcctttac ttccgggtgc atggggcagg tatattcgct ccccttcttt tcctcttgtt
     1501 ctccttcgtt catcgtggcc tcccggtcca tggactcgac ttcctcacat cc
//
LOCUS       DASTJJ010000324         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_536058, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000324 DASTJJ010000000
VERSION     DASTJJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536058"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFR02_04065"
     CDS             complement(<1..528)
                     /locus_tag="VFR02_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9869660.1"
                     /translation="MASTPRLNLVQKIIQAHLVSGKPLAGEEVGIKIDQTLTQDATGT
                     MAYLQFEAIGVPQVRTKLSVSYVDHNMLQTGFENADDHRYLQSVAAKHGVYFSRPGNG
                     ICHQVHLERFAVPGMTLLGSDSHTPTCGGLGELSIGAGGLDVAVAMGGGPFYMTMPKV
                     LRVELKGKLQPWVTAK"
     gene            704..925
                     /locus_tag="VFR02_04070"
     CDS             704..925
                     /locus_tag="VFR02_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET9869661.1"
                     /translation="MSQAVFNIDGMTCDHCVRAVTEALKGVAGVREAEVNLKDRLARV
                     TYEGGLETPAVLRAVEEAGYRASPAESRP"
     gene            922..>1366
                     /locus_tag="VFR02_04075"
     CDS             922..>1366
                     /locus_tag="VFR02_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET9869662.1"
                     /translation="MTAPAPAEAAAHPLILSVSGMTCASCAARIEKRLRQVPGVREAN
                     VNFAAQKAYVALEPGFTDPAPLEAAVAQAGYGAEVHHPRPRGVPREPSDLRWRVLVGA
                     AGAFPFLANHLYDLVGHAAIPLPVQFGLALPVYALTGWIFHRPALL"
BASE COUNT          221 a          443 c          476 g          226 t
ORIGIN      
        1 cttggcggtc acccagggct gcagcttgcc cttgagctcg acccgcaaca ccttcggcat
       61 ggtcatatag aaggggccgc cgcccatggc caccgccacg tccaggccgc ccgcgccgat
      121 ggaaagctcg cccaagccgc cgcaggtggg ggtgtgggaa tccgacccca aaagggtcat
      181 gcccggcacc gcgaagcgct ccaaatgcac ctggtggcag atgccgttgc cggggcggga
      241 aaagtagacc ccgtgcttgg cggcgacgga ttgcaggtac cggtggtcgt cggcgttctc
      301 aaagccggtc tgcagcatgt tgtggtccac gtaggacact gaaagtttgg tccggacctg
      361 gggcacgccg atggcctcga actggaggta ggccatggtg ccggtggcgt cctgggtgag
      421 ggtctggtcg atcttgatgc ccacttcctc gccggccagg ggcttgccgg acaccaggtg
      481 ggcctggatg atcttctgga ctaggttcaa acggggggtc gacgccatgg gaatagcctc
      541 catcggttaa tgttcggcgg gagcgttgtt ctttcaagaa tggaaaggcc tattatggaa
      601 ggggggtgaa ggcgaattca acccaaaaga ccccccggcc gtccttgcat ttaacggata
      661 aaattccgaa actgatgaaa tcccggggga aaaggaggac ggcatgtccc aagcggtctt
      721 caacatcgac ggcatgacct gcgaccattg cgtgcgcgcc gtgaccgagg ccttgaaggg
      781 ggtcgcgggc gtgcgggaag ccgaggtcaa cctgaaggac cgcctcgccc gggtgaccta
      841 tgaggggggc ctggagaccc cggccgtcct gcgggccgtg gaggaggcgg ggtaccgcgc
      901 ctcgcccgcc gaatcccggc cgtgacggcc cccgcgccgg cggaagcggc cgcccatccc
      961 ctgatcctgt cggtcagcgg catgacctgc gcctcgtgcg cggcccgcat cgagaagcgc
     1021 ttgcgccagg tgccgggggt gcgggaagcc aacgtgaact tcgccgccca gaaggcctat
     1081 gtggccctgg agccgggctt caccgacccg gcgccgctgg aagccgccgt ggcccaggcg
     1141 ggttatgggg cggaagtcca tcatccccgc ccccgcggtg tcccccggga accgtcggac
     1201 ctgcgttggc gcgtcctggt gggggccgcg ggcgccttcc ccttcctggc caaccacctc
     1261 tatgacctgg tggggcatgc cgccatcccg ctgcccgtgc agttcggcct ggccctgccc
     1321 gtctacgcgc tgaccgggtg gatcttccac cggccggcgc tgctgc
//
LOCUS       DASTJJ010000325         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_536067, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000325 DASTJJ010000000
VERSION     DASTJJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536067"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="lpxC"
                     /locus_tag="VFR02_04080"
     CDS             complement(<1..758)
                     /gene="lpxC"
                     /locus_tag="VFR02_04080"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET9869663.1"
                     /translation="MTARPAPVKQRTLKKAFQFEGKGLHTGALCRVTVSPAPAGSGIR
                     FFRQDLGAEIPLSPFGVTGTARGTTLTGEKDAKIHTIEHFLAAVRGFALDNLRVEMTG
                     EEMPILDGSAVPFCEAIRGAGWVEQEADADPLVVTEVLELSFGDVRLKAEPADRLELD
                     VTISFPFPGLEHQNLKFGLDAGSFERELAPARTFCFEKEVEALRQQGLIKGGGLDCAL
                     VMGEKGVLNGPLRFDDEFVRHKTLDLLGDLTLLNR"
     gene            complement(748..1791)
                     /gene="lpxD"
                     /locus_tag="VFR02_04085"
     CDS             complement(748..1791)
                     /gene="lpxD"
                     /locus_tag="VFR02_04085"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET9869664.1"
                     /translation="MGRAFTLKELADQLGGSFTGDPQLALRGIAPLSAAGEGDLTFVT
                     HPRYAPQAAATRASAVLCDKAMPGVDKPFLMVPNPYLTLAKAIALFHPAKAYAPGVRP
                     GAWVDPGAQVDPGAVVFDGVTVMAGARVGRGSVLFPGVFLGEGSVVGEDCLIHPNVTI
                     REGCRLGDRVILQPGVVVGADGFGFARDKDRYVKIPQVGNVEIGDDVELGANVCVDRA
                     VLGTTRIGEGTKLDNLIQVGHNVAIGRHGVIAAQTGIAGSTTVGDFTMMGGQVGLAGH
                     LQIGDNVTLATRTGVMEDIPDKGLYWGSPSAPMAEEMKNVAAYRQLPALMKRVRQLEK
                     ALEKLREDAGHDR"
     gene            complement(1795..>2016)
                     /locus_tag="VFR02_04090"
     CDS             complement(1795..>2016)
                     /locus_tag="VFR02_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET9869665.1"
                     /translation="DLIQQKQQIMAELKGAEDQMTDDILSQIKAIVVKVAKAKGYDAV
                     LDSEKTILVTDPVDLTDEVIKAFPSGGSN"
BASE COUNT          306 a          703 c          673 g          334 t
ORIGIN      
        1 cggttgagca gcgtcaggtc gcccaacagg tccaacgtct tgtggcggac gaactcgtcg
       61 tcgaagcgca ggggcccgtt caagaccccc ttctcgccca tcacgagggc gcaatccagg
      121 ccgccgccct tgatcaggcc ctgttggcgc agggcctcca cttccttctc gaagcaaaag
      181 gtgcgggcgg gggccagttc ccgctcgaag ctccccgcgt ccaggccgaa cttgaggttc
      241 tggtgctcca agccggggaa ggggaaggaa atggtcacgt ccagttccag ccggtcggcg
      301 ggctcggcct tgagccgcac gtccccgaag gaaagctcca ggacctcggt gaccaccagg
      361 gggtccgcgt cggcctcctg ctcgacccag ccggccccgc ggatggcctc gcaaaacggg
      421 accgcgctcc cgtccaggat gggcatctcc tcgccggtca tctccacgcg caggttgtcc
      481 agggcgaagc cccggaccgc ggccaggaag tgctcgatgg tgtggatctt ggcgtccttc
      541 tcgccggtca gggtggtgcc ccgggccgtg cccgtcacgc cgaagggcga cagggggatc
      601 tcggcgccca ggtcctggcg gaaaaaacgg atgccggacc cggcgggggc cggcgagacg
      661 gtcacccggc acagggcgcc cgtgtgcagg cccttgccct cgaattggaa cgccttcttc
      721 agggtgcgtt gtttgacggg ggcgggccta gcggtcatga ccggcgtcct cccgcagttt
      781 ctccagcgcc ttttccagct gccgcacgcg cttcatgagc gcgggcaact gccggtaggc
      841 cgcgacgttc ttcatttcct ccgccatggg cgccgagggc gaaccccaat agaggccctt
      901 gtccgggatg tcctccatca cgcccgtgcg ggtggccagg gtgacgttgt cgccgatctg
      961 aaggtggccc gccaggccca cctggccgcc catcatggtg aagtcgccca cggtggtcga
     1021 ccccgcgatg cccgtttggg ccgcgatgac gccgtgcctg ccgatggcca cgttgtggcc
     1081 gacctggatg aggttgtcca atttggtccc ttccccgatg cgggtggtgc ccagcacggc
     1141 gcggtccacg cagacgttgg cgcccagctc gacgtcgtcg ccgatctcca cgttccccac
     1201 ctgggggatc ttgacgtaac ggtccttgtc gcgggcgaag ccgaagccgt cggcgcccac
     1261 caccacgccg ggctgcagga tgacccggtc ccccagccgg cagccctccc ggatggtgac
     1321 gttgggatgg atcaggcaat cctcgcccac caccgacccc tcgcccagga acaccccggg
     1381 aaaaagcacg ctgccccggc ccacccgggc cccggccatg acggtcacgc cgtcaaagac
     1441 caccgccccc gggtccacct gggcccccgg gtccacccag gcccccggcc ggacgcccgg
     1501 cgcgtaggcc ttggccggat ggaagagggc gatggctttg gccagggtca ggtagggatt
     1561 gggcaccatc aggaagggct tgtccacccc cggcattgcc ttgtcgcaaa ggacggccga
     1621 ggcccgggtg gcggcggcct ggggcgcgta gcggggatgg gtgacgaagg tcaggtcccc
     1681 ttccccggcc gccgaaagcg gcgcgatgcc acgcaaagcc agttgggggt ccccggtgaa
     1741 ggagcccccc aattggtccg ccagttcttt cagcgtgaag gcacggccca tcggttaatt
     1801 cgaaccgccg gacgggaagg ccttgatgac ctcgtcggtc aggtccaccg gatcggtgac
     1861 caggatggtc ttctccgaat ccagcacggc gtcatagccc ttggccttgg ccaccttgac
     1921 cacgatggcc ttgatctggg agaggatgtc gtcggtcatc tggtcctccg cgcccttcag
     1981 ctccgccatg atctgctgtt tttgttggat caggtc
//
LOCUS       DASTJJ010000326         1489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_413894, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000326 DASTJJ010000000
VERSION     DASTJJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413894"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..488)
                     /locus_tag="VFR02_04095"
     CDS             complement(81..488)
                     /locus_tag="VFR02_04095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9869666.1"
                     /translation="MALSQHAYDAVIRESHLDSFGHVNNAAYMVLFEEARWEWITQNG
                     YGLPVIQRTRQGPVVLEATVKFKRELTLREKITITSEMTDYRGKIGHCAQKMVKADGT
                     VACEALFVVGLFDLTARKLVDPTPAWRRAIGLD"
     gene            601..>1489
                     /locus_tag="VFR02_04100"
     CDS             601..>1489
                     /locus_tag="VFR02_04100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET9869667.1"
                     /translation="MDRVIAHLDMNSFFVSCERRVDPSLNGKPVVVGGRKGERGVVAS
                     ASYEARRFGVKSGMPIVTAEKLCPRAVFVPGHHRLYGKYSRKVYVLLRRLAPVVEYAS
                     IDEFYLDFTGCEALYGKDLMALARRVRDAVLERSGLSCTVALASNKYVAKIAGKTVKP
                     DPSALGKVHQDTGVIVVPAGDEQKFLSALSIQRLHGAGEKTQPRLAEMRLRTIGDILA
                     LPLDKLKKAFGPSAGQWLYDAARGRDDSPVAPFHPAKSVGNETTFGADTDDLALARRT
                     LAWLAEKSCYRLRRLGQKAR"
BASE COUNT          235 a          493 c          520 g          241 t
ORIGIN      
        1 tgggaaaggg aaggggccca ggtgcgggtc ctggaccggg aggccgccct gacgggcctg
       61 gcccgggtct tttcgggggg ctagtccagc ccgatggccc ggcgccaggc gggcgtgggg
      121 tcgaccagtt tccgggccgt caggtcgaag aggcccacca cgaagagggc ctcgcaggcc
      181 accgtgccgt ccgccttcac catcttctgg gcgcaatggc cgatcttgcc ccggtagtcc
      241 gtcatctcgg acgtgatcgt gatcttttcc cgcagcgtca attcccgctt gaacttcacg
      301 gtggcttcca gcaccaccgg cccctggcgg gtccgctgga tcacgggaag accgtagccg
      361 ttctgggtga tccattccca ccgggcctcc tcgaacagca ccatgtaggc cgcgttgttc
      421 acatggccga aactgtccag atgggattcc cggatgacgg cgtcataggc gtgttgggac
      481 agggccatag ggctccttgg cagcggggtt ccgacgccca aagggtaggg ggctgggcgg
      541 gggcgcgtca agcccgccgg gcttgcgcgg ggagggccgg atggtatact ggcgtatacc
      601 atggaccgcg tcatcgccca cctggacatg aattccttct tcgtttcctg cgaaaggcgg
      661 gtggacccct ccttgaacgg gaagcccgtg gtggtgggcg gccgcaaggg ggagcggggc
      721 gtggtggcct cggcctccta cgaggccagg cggttcggcg tgaagtccgg catgcccatc
      781 gtgaccgccg aaaaactctg cccccgggcg gtcttcgtgc cgggccacca ccggctctac
      841 ggaaaatatt cccgcaaggt ctacgtgctc ctgaggcggc tggcccccgt ggtggaatac
      901 gcctccatcg acgagttcta cctcgatttc acgggctgcg aggccctcta cgggaaggac
      961 ctcatggccc tggcgcgccg ggtgcgcgac gccgtgctgg agcgctcggg cctttcctgc
     1021 acggtggcgc tggcctccaa caagtacgtg gccaagatcg cgggcaagac ggtcaagccg
     1081 gacccttcgg ccctggggaa ggtccaccag gacacgggcg tcatcgtggt gccggccggc
     1141 gacgagcaga aattcctgtc ggcgctttcc atccaacgcc tgcacggggc gggggaaaag
     1201 acccagcccc ggctggccga gatgcggctg cgcaccatcg gggacatcct ggccctcccg
     1261 ctggacaagc tgaaaaaggc cttcggcccc tcggcgggcc aatggcttta cgacgcggcc
     1321 cggggccggg acgattcgcc ggtggcgccc ttccatcccg ccaagagcgt ggggaacgag
     1381 accaccttcg gggccgacac cgacgacctg gcgctggcgc gccgcaccct ggcctggctc
     1441 gcggaaaaaa gctgctaccg cctgcggcgg ctgggccaaa aggcccgca
//
LOCUS       DASTJJ010000327         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_536219, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000327 DASTJJ010000000
VERSION     DASTJJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536219"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFR02_04105"
     CDS             <1..528
                     /locus_tag="VFR02_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9869668.1"
                     /translation="VQCGSDAILKRMSRKYSVAHYLERARKLQAAVPDIALTTDLIVG
                     FPGETEADFQGTLRLLEEVRYDLVFSFKYSPRTGTPAARMLDPVEEPVKDERLARLNA
                     LAWKHASEKHAARVGKVEEVLVDGPADRTPGASYGKTRQNKTLVITNGVFPTGSLVRV
                     KVEGSKIAALYGTAV"
     gene            688..1860
                     /gene="bamD"
                     /locus_tag="VFR02_04110"
     CDS             688..1860
                     /gene="bamD"
                     /locus_tag="VFR02_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9869669.1"
                     /translation="MKKTILLLTILAAAPALAQTPTPAGESPQALFDKAKQLFDAKQY
                     QEARDSLGRLVAKYPTESFVPQARILLADLEEDFSSATARFQELAAEYEGSPEGAEAQ
                     KDLADRYYLADKYAEAGEAYRDYLAQYPKSPDLAEIHYWLGCAYLAQDQSDPAIEQFK
                     KVADGDAKAPWVSKSRVGLGNAYFKKGQYDQAERQYLRILQSDPGYDELNSVYDKLGQ
                     TFEMEKKDHQAYAAYQSLLDRYPNALEAADAKARMEALARVHPDYAEEAEGDTPTPTP
                     TPVGAEPPPEPTGTPAAEAVPTLTPTVVPPARPKPFHVQVGVFSQKAYADKAEEAVRR
                     AGAPVFVVEAQGNASPYPYYKVRSGDYASRAEAEKAAKKLGGLLKQQVIVVEDQGD"
     gene            2092..>2373
                     /locus_tag="VFR02_04115"
     CDS             2092..>2373
                     /locus_tag="VFR02_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9869670.1"
                     /translation="MDDLTPMMRQYLEIKRAHKDEMLFFRLGDFYEMFFEDAKTAARV
                     LNLTLTARQGGNGQKVPMAGIPFHAADNYIARLVKSGHSIAICEQTEEAG"
BASE COUNT          471 a          802 c          743 g          357 t
ORIGIN      
        1 gtgcaatgcg ggtccgacgc catcctcaag cgcatgagcc gcaaatacag cgtggcccat
       61 tacctggaac gggcccgcaa gctccaggcc gccgtccccg acatcgccct gaccacggac
      121 ctcatcgtgg gcttccccgg cgagaccgag gcggacttcc aggggacgct gcgcctgctg
      181 gaggaggtcc gttacgacct ggtcttcagc ttcaagtact cgccccgcac cggcaccccg
      241 gccgcccgca tgctggaccc ggtggaggag ccggtgaagg acgagcgcct ggcccgcctg
      301 aacgccctgg cctggaagca cgcctccgaa aagcacgccg cccgggtggg gaaggtggag
      361 gaggtgctgg tggacggccc cgcggaccgc accccgggcg ccagctacgg caagacccgg
      421 cagaacaaga ccctggtgat caccaacggg gtcttcccga cgggcagcct ggtgcgggtg
      481 aaggtggaag gcagcaagat cgccgccctc tacggcacgg cggtgtaaat ataaaagtac
      541 ggcgcacgca gagacgccaa ggacggcaaa aacaacgtta tgattttgct ccaaatttaa
      601 aaatccttaa ctttgcgtct tggcgtctcc gcgttggctt ttttctttgg tttaactttg
      661 gttcaagttt ttttacaaaa ggcgaacatg aaaaagacca tcctgctgct gacgatcctc
      721 gcggccgcgc cggcgctggc ccagaccccc acgcccgccg gggaaagccc ccaggccctc
      781 ttcgacaagg ccaagcagct ctttgacgcc aagcaatacc aggaagcccg tgattcgctg
      841 ggccggctgg tggccaagta ccccacggaa agcttcgtgc cccaggcccg catcctgctg
      901 gcggacctgg aagaggactt ttcctcggcc acggcccggt tccaggaact ggccgccgag
      961 tacgaggggt ccccggaggg cgcggaggcc cagaaggacc tggcggaccg ttattacctg
     1021 gcggacaaat acgccgaggc gggcgaggcc taccgggatt acctggccca atatcccaag
     1081 agcccggacc tggcggagat ccactattgg ctgggctgcg cctacctggc ccaggaccag
     1141 tcggacccgg ccatcgagca gttcaagaag gtggccgacg gcgacgcgaa ggccccctgg
     1201 gtctccaaga gccgggtggg gttgggcaac gcctatttca agaagggcca gtacgaccag
     1261 gccgagcggc agtacctgcg catcctgcag tcggacccgg gctatgacga gctgaactcg
     1321 gtctatgaca agctgggcca gaccttcgag atggaaaaga aggaccacca ggcctacgcg
     1381 gcctaccaat cgctgctgga ccgctacccc aacgcgctgg aagccgccga cgccaaggcc
     1441 cgcatggagg ccctggcccg cgtgcacccg gactacgccg aggaggccga gggcgacacc
     1501 cccacgccca cccccacccc cgtgggcgcc gaacccccgc ccgagcccac cgggaccccg
     1561 gccgccgaag ccgtccccac gctgaccccc acggtcgtcc cgccggcccg gcccaaaccc
     1621 ttccacgtgc aggtgggggt cttcagtcag aaggcctacg ccgacaaggc cgaggaggcg
     1681 gtgcggcgcg cgggcgcccc cgtcttcgtg gtggaggccc aggggaatgc cagcccctat
     1741 ccctactaca aggtgcgctc gggcgactac gcctcccgcg ccgaggccga gaaggccgcc
     1801 aaaaagctgg gcggcctcct gaagcagcag gtcatcgtgg tcgaggacca gggcgactga
     1861 aaaggcggac tggatccacc gccacaccca agggtggccc cgttacccaa acatcaatgt
     1921 tggggcaggc gccaagacac caagggaggc ggaagctgaa gccggccttc ccggtttcac
     1981 agactctttt tccttcatgg atcatcaata tttcacttgg tgtcttggtg ccttggtggt
     2041 gaaaatggtc ctttcttttg atccaatctt tcacggaagg ctgagggatc catggacgac
     2101 ctgacgccca tgatgcgcca gtacctggag atcaagcgcg cccataagga cgagatgctg
     2161 ttcttccgcc tgggcgactt ctacgagatg ttcttcgagg acgccaagac cgccgcccgg
     2221 gtgctgaacc tcaccctgac cgcccgccag ggcggcaacg gccagaaggt gcccatggcg
     2281 ggcatcccct tccacgcggc ggacaactac atcgcccggc tggtcaagag cggccattcc
     2341 atcgccatct gcgagcagac cgaggaggcg ggg
//
LOCUS       DASTJJ010000328         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_414002, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000328 DASTJJ010000000
VERSION     DASTJJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414002"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFR02_04120"
     CDS             <1..432
                     /locus_tag="VFR02_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9869671.1"
                     /translation="RDPDQSEIARAARIKLEKVHKLQRLAQRSLSLEVPVSDDPNAKS
                     MGDFIEDKGVESPAQSTFRRLRSEKIHRLIHSLNDKEQQVLAMRFGFEAGSPRTLEQT
                     GKCLGVTRERIRQIEEKALQKIRSLMRASLEEYRDLLHEFA"
     gene            530..1042
                     /locus_tag="VFR02_04125"
     CDS             530..1042
                     /locus_tag="VFR02_04125"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9869672.1"
                     /translation="MDLKRFIRDVPDFPKPGILFKDITPLLRAPEAFAEVIRLFADRY
                     RTSGLDAVVAVESRGFILGGALARELKVGFVPVRKPGKLPWKAIQESYALEYGTDKLE
                     IHEDALAGGGKALILDDVLATGGTAAATAELVRKAGGSVHEAAFLIDLTFLKGREKLK
                     NTPVYSIIQY"
     gene            1062..1138
                     /locus_tag="VFR02_04130"
     tRNA            1062..1138
                     /locus_tag="VFR02_04130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1096..1098,aa:Arg,seq:cct)
     gene            1178..1876
                     /locus_tag="VFR02_04135"
     CDS             1178..1876
                     /locus_tag="VFR02_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869673.1"
                     /translation="MTTWNPNQDPIHAGPTNAWTKLLAKAKAHQEALAVGGIIVIILA
                     LFVPYYLNGRARSEKDAAGVLNLGQYYLRANVDPKSGPFKSLGEKYQQCLQTFQRIVS
                     DYPGTSSSRVARYYLAECQFSLGDYAKAFSSFDQASLDLKGSPLADQALVGRVVSLMG
                     ESQWSQAQEAALAFLSDHPGSFLAPEMKLDLSDIDLKLKQKEESRKVLKELAEASPDT
                     DWGREAARRLKEQS"
BASE COUNT          376 a          623 c          587 g          299 t
ORIGIN      
        1 cgggatccgg accagtccga gatcgcccgc gcggcccgca tcaagctgga gaaggtccac
       61 aagctgcagc ggctggccca acgcagcctg tcgctggagg tcccggtgag cgacgacccc
      121 aacgccaaat ccatgggcga cttcatcgag gacaagggcg tcgaaagccc cgcccagtcc
      181 accttccgcc gcctgcgctc ggagaaaatc caccgcctga tccattcgct caacgacaag
      241 gaacagcagg tcctggccat gcggttcggc ttcgaggccg ggtcgccccg caccttggag
      301 cagacgggga aatgcctggg cgtgacccgg gaacgcatcc gccagatcga ggaaaaggcc
      361 ctgcagaaga tccgctccct catgcgcgcc agcctggaag aataccggga cctgctgcac
      421 gaattcgcct gaccccccgc cgatccccca aaaatcgcct ggaattcgtc cccgtttgtt
      481 gtaccctgga gcccgccgag ccgccccatc cccatccccg aggccgccca tggacctgaa
      541 gcgcttcatc cgggacgttc ctgatttccc caagccgggc atcctgttca aggacatcac
      601 gcccctgttg cgggcgccgg aggccttcgc ggaggtcatc cggctcttcg cggaccgcta
      661 ccggacttcg ggcctggacg ccgtggtggc cgtggaatcg cgcggcttca tcctgggcgg
      721 ggccttggcg cgcgagttga aggtgggctt cgtgccggtg cgcaagcccg gcaaactgcc
      781 ctggaaggcc atccaggaaa gctacgccct ggagtacggg acggacaagc tggagatcca
      841 cgaggacgcg ctggccggcg gcgggaaggc cttgatcctg gacgacgtgc tggccaccgg
      901 ggggacggcg gccgccaccg ccgaattggt gcgcaaggcc gggggcagcg tgcacgaggc
      961 ggccttcctg atcgacctga ccttcctcaa ggggcgcgaa aaattgaaaa acacccccgt
     1021 ctattctata atccagtact aattttcaag gtcattcccg ggccctcgta gctcagttgg
     1081 atagagcgac cctttcctaa agggtaggtc acgcgttcga tccgcgtcga gggtaccatt
     1141 acgtaagaac ggtcgaatcc ttcatcaaag gcggaacatg acaacctgga accccaacca
     1201 agacccgatc catgccggtc cgacgaacgc ttggaccaag ctgctggcca aggccaaggc
     1261 ccaccaagag gcgttggcgg tgggcggcat catcgtcatc atcctggcgc ttttcgtgcc
     1321 ctattacctc aacggccggg cccggtcgga gaaggacgcc gcgggcgtcc tgaacctggg
     1381 gcaatattac ctgcgcgcca acgtggaccc caagagcggg cccttcaaga gcctggggga
     1441 aaagtaccaa cagtgcctgc agaccttcca acgcatcgtg agcgattacc cggggacctc
     1501 gtccagccgc gtggcccgtt attacctggc cgagtgtcag ttcagcctgg gggattacgc
     1561 caaggccttc agcagtttcg accaggcctc gctggacctg aagggaagcc ccctggcgga
     1621 ccaggccctg gtgggccgcg tggtcagcct gatgggggaa tcccagtgga gccaagccca
     1681 agaggcggcc ctggccttcc tgagcgacca tccgggcagc ttcctggcgc ccgagatgaa
     1741 gctggacctg tcggacatcg accttaaatt gaagcagaag gaagaatcac ggaaggtctt
     1801 gaaggaactg gcggaagcct ctcccgatac ggattggggc cgcgaggcgg cccgccgcct
     1861 caaggaacag tcctaaaggc ccgcc
//
LOCUS       DASTJJ010000329         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_414055, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000329 DASTJJ010000000
VERSION     DASTJJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414055"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VFR02_04140"
     CDS             <1..1355
                     /locus_tag="VFR02_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869674.1"
                     /translation="APLFHRPGNRVTLTRNQKILLALLGAYLFRLGFGLSMPFWPDDQ
                     KQTYLIGLKCYTTGTWPYYGPDVNGSETAYTSQLPGALEGLVIALPLKLLPIPEAPFI
                     LLNLMSLFAIGLLTWYFSKKVPLLSPGFIFGSLAVLPWDLQESTSIINPSWCLFGSAL
                     FFLGFFEALPGFKMGLVRPALCNALMGFALFWVMQFHMSWMYLAALMGVSLFLQARER
                     PAALGPALGCMALGALPSFAFMAPTLLKYGIPKGHNGSGFESAFNFGNFIQFFTILAR
                     YLSLACYELPRFLGLTTKLRFEFIRESVFTFLPGMFLWVLGLVQAVVLFVMGFRKDHP
                     LKDWPVVRNVTFVSFLIIWASFWFTVKWPLAHIYYTCFPLVFLYSLYGWSRFAGSKGW
                     RLFAKVYLASAFIFQIAYAVRAGGHPELTLYHDRGKVVQAIQQKDYRVMGERRENSYY
                     "
     gene            1485..3098
                     /locus_tag="VFR02_04145"
     CDS             1485..3098
                     /locus_tag="VFR02_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HET9869675.1"
                     /translation="MGACGPKGPGTAFRPVRLLALALLLAGPAGAGDVHFLILPSQTI
                     GPISPYIYGLNDQDPAGLNVTLRRLGGNRLTGYNWTNNASNAGSDWHQTSDDWLCADH
                     AGDGDSGKPGALAFHFVEQDQAEGLASLLTLPMAGYVAADKDGEVTQAQTAPSPRWAR
                     ELPRKGAPFTLDPDPASKTVYDDEFVNFLKSRFGPAGQGGPVFYDLDNEPALWPSTHP
                     RLHPAKPTYREMVERTQALASAVLDVDPSAVLFGGVMYGWQEMRTLQDAPDSAPFDQT
                     YGTYTDYYLAQMREAEKRTGRRLVQVLDLHWYPEAQGAGKRITQGDTSPDSVEARLQA
                     PRSLWDPGYVEKSWITRDSTQGAPIALIPWLQAKIDRWYPGTKLAFSEYDYGAGDHVS
                     GGLAQADVLGIFGKYGVFEASYWGDLKAYNRAAFDLYRDYDGHDSTFGGEAVSAGTEN
                     VVETSVYAAVDPASPGRLWIVALNKDPQRTLHGVFEVRGQTPYARYQAYGFNARSPAV
                     KPVRSGSLDQGRFDLDLPPLSATIFVCAD"
     gene            complement(3111..>3440)
                     /locus_tag="VFR02_04150"
     CDS             complement(3111..>3440)
                     /locus_tag="VFR02_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869676.1"
                     /translation="VAEVAPGSYGTRVFDSARYGAGVRAKGSPYAAFTQQVESLARAE
                     AAKARPPREVAELILRVVQGRALKPVYLAGPDAKALNFLKWLLPDGIFEWLFSRFFPW
                     SRPPKRR"
BASE COUNT          547 a         1260 c         1032 g          601 t
ORIGIN      
        1 cggcgcccct attccaccgt ccggggaacc gcgtgaccct cacccgaaac caaaagatcc
       61 tgctggccct gctgggggcc tacctcttcc gcctgggctt cggcctgtcc atgcccttct
      121 ggcccgacga ccaaaaacag acctacctga tcggcctcaa gtgctacacc accggcacct
      181 ggccctatta cggcccggac gtcaacggca gcgagaccgc ctataccagc cagttgcccg
      241 gggccctgga aggcctggtc atcgccctgc ccctcaagct gctgcccatc cccgaggcgc
      301 ccttcatcct gttgaacctg atgtcgctct tcgccatcgg cctcttgacc tggtattttt
      361 cgaagaaggt ccccctgctg tccccgggct tcatcttcgg gtccctggcg gtgctgccct
      421 gggacctgca ggaatccacc agcatcatca acccctcctg gtgcctcttc ggcagcgccc
      481 tctttttcct gggctttttc gaggccctgc cgggcttcaa gatggggctg gtccggcccg
      541 cgctctgcaa cgccctcatg ggtttcgccc ttttctgggt catgcagttc cacatgtcct
      601 ggatgtacct ggcggccctg atgggcgtct cgctcttcct gcaggcccgc gaaaggcccg
      661 ccgccctggg cccggcgctc ggttgcatgg ccttgggcgc gctgccttcc ttcgccttca
      721 tggcccccac actgctcaag tacggcatcc ccaagggcca caacggctcg ggtttcgagt
      781 cggccttcaa cttcggcaac ttcatccagt tcttcaccat cctggcccgc tacctgagcc
      841 tggcctgcta tgaactgccc cggttcctgg ggctcaccac caagctccgg ttcgagttca
      901 tccgggagtc ggtcttcacc ttcctgcccg ggatgttcct ctgggtcctg ggcctggtcc
      961 aggccgtcgt cctcttcgtc atgggcttcc ggaaggacca tcccctgaag gactggcccg
     1021 tcgtgcgcaa cgtgaccttc gtgagcttcc tcatcatttg ggcgagcttc tggttcaccg
     1081 tcaagtggcc cctggcccac atctattaca cctgcttccc gctggtcttc ctttattcgc
     1141 tctacggctg gagccgtttc gccggctcca agggttggcg cctcttcgcc aaggtctacc
     1201 tggcctcggc cttcatcttc cagatcgcct acgccgtgcg ggcgggcggc caccccgagc
     1261 tgaccctcta ccacgaccgg ggcaaggtcg tgcaggccat ccagcagaag gattaccgcg
     1321 tcatgggcga acgccgggaa aacagctatt actaggaatt ttttcaccgc agggacgcag
     1381 aggcgcaaag atagggccat ggtcaaggat cgaggaaaat gaacacgttt ttctccgcgt
     1441 ccccgtgccg ccccaacctt ccattgttga aaaaggggcc tcccttgggc gcctgcggtc
     1501 caaaaggccc tggaacggct tttaggccgg tccggctcct ggccttggcc ctcctgctgg
     1561 ccggccccgc gggggccggg gacgtccatt tcctcatcct gccttcccaa accatcgggc
     1621 ccatcagccc ctacatctac ggcctgaacg accaggaccc ggcggggctc aacgtgacgc
     1681 tgcggcgcct gggcggcaac cggctcaccg gctacaactg gaccaacaac gcctccaacg
     1741 cgggcagcga ttggcaccag acgagcgacg actggctctg cgccgaccac gcgggcgacg
     1801 gcgacagcgg caagcccggg gccctggcct tccacttcgt tgaacaggac caagccgagg
     1861 gcctggcctc gctcctcacc ctgcccatgg cgggctacgt ggcggccgac aaggacggcg
     1921 aggtgacgca ggcccagacc gcgcccagcc cccgctgggc ccgggagctg ccccgcaagg
     1981 gcgccccctt cacgctcgat cccgaccccg cctccaagac cgtctacgac gatgagttcg
     2041 tgaacttcct gaaaagccgc ttcggccccg cgggccaagg cggcccggtc ttttacgacc
     2101 tggacaacga gccggccctc tggccttcca cccatccccg cctgcacccg gccaagccca
     2161 cctaccggga aatggtggaa cgcacccagg ccctggcctc ggccgtgctg gacgtggatc
     2221 cctcggcggt gctcttcggc ggggtcatgt acggctggca ggagatgcgg accctgcagg
     2281 acgcccccga cagcgcgccg ttcgaccaga cctacggcac ctacacggac tattacctgg
     2341 cccagatgcg ggaagcggaa aaaaggacgg gccggcgcct ggtccaggtg ctggacctgc
     2401 actggtaccc ggaagcccag ggcgccggca agcgcatcac ccagggcgac acctcgcccg
     2461 actcggtgga ggcccgcctg caggcgcccc gcagcctctg ggacccgggc tacgtggaaa
     2521 aaagctggat cacccgggat tccacccagg gcgcgcccat cgccctcatc ccctggctgc
     2581 aggccaagat cgaccgctgg tatccgggca ccaagctcgc cttcagcgaa tacgactacg
     2641 gcgcggggga ccacgtgtcg ggcgggctgg cccaggcgga cgtgctgggc attttcggca
     2701 agtacggcgt ctttgaggct tcctactggg gggacctgaa ggcctacaac cgggcggcct
     2761 tcgacctgta ccgcgattac gacggacacg actccacctt cggcggcgag gccgtctcgg
     2821 cgggcacgga gaacgtggtg gagacctcgg tctacgcggc ggtggatccg gcttccccgg
     2881 gccgcctctg gatcgtggcc ttgaacaagg accctcaacg gacgctgcac ggggtctttg
     2941 aggtgcgggg ccaaacccct tatgcccgct accaagccta cggtttcaac gcccgatccc
     3001 cggcggtcaa gccggtccgg tcggggtcgt tggaccaggg ccgcttcgac ctggacctgc
     3061 cgccgctctc cgccacgatc ttcgtctgcg cggattgagg cccgggcggc tcaccgccgc
     3121 ttgggcggac ggctccaggg aaagaacctc gaaaagagcc actcgaagat cccgtcgggc
     3181 agcaaccact tgaggaaatt cagcgccttc gcgtccggcc cggccaagta gaccggcttc
     3241 aaggcccggc cctggaccac ccggaggatg agttcggcca cttcccgggg cggccgcgcc
     3301 ttggcggcct cggcccgcgc cagggactcc acctgctgcg tgaaggcggc gtagggcgat
     3361 cccttggccc gaacccccgc gccgtaacgg gccgaatcga agacccgcgt gccgtaggaa
     3421 cccggggcca cctcggccac
//
LOCUS       DASTJJ010000330         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_170517, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000330 DASTJJ010000000
VERSION     DASTJJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170517"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /gene="carB"
                     /locus_tag="VFR02_04155"
     CDS             <1..546
                     /gene="carB"
                     /locus_tag="VFR02_04155"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889527.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HET9869677.1"
                     /translation="VFPFNRFPGVDTVLGPEMKSTGEVMGIDTDFGMAFAKSQMGANS
                     ALPMAGTVFVSVKNKDKRSVIFIAKRLSDMGFKLVATAGTAQALTSNGMEVTVVRKVH
                     EGRPNVVDLIKNGGIQLIMNTPAGKGPRSDDFQIRRTAVVYGVPCITTLSACAAAANG
                     IEALKKKEIRVKPLQDHHAGV"
     gene            665..979
                     /locus_tag="VFR02_04160"
     CDS             665..979
                     /locus_tag="VFR02_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869678.1"
                     /translation="MKKTQAVLAALLALSFASVSLAEDHPMSPKHRLVRQHARIEAGV
                     KNGSVTPAEHKKLAKEGRHINRERRRDLKKDGGKLTPADKAKLERQENVRSAQIAKDK
                     HN"
BASE COUNT          226 a          365 c          333 g          181 t
ORIGIN      
        1 gtcttcccct tcaaccgttt cccgggcgtc gacaccgtgc tggggcccga gatgaagtcc
       61 accggcgagg tcatgggcat cgacaccgat ttcggaatgg ccttcgccaa gagccagatg
      121 ggcgccaatt cggccctgcc catggccggc acggtcttcg tgtcggtcaa gaacaaggac
      181 aagcgctcgg tcatcttcat cgccaagcgc ctttcggaca tgggcttcaa gctggtggcc
      241 accgccggca cggcccaggc cctcaccagc aacggcatgg aagtgaccgt ggtgcgcaag
      301 gtgcacgagg ggcggcccaa cgtggtggac ctcatcaaga acgggggcat ccagctcatc
      361 atgaacacgc ccgcgggcaa ggggccccgg tcggacgatt tccagatccg ccgcacggcg
      421 gtggtctacg gcgtgccctg catcaccacg ctgtcggcct gcgcggcggc ggccaacggc
      481 atcgaggcct tgaagaagaa ggagatccgc gtgaagcccc tgcaggacca ccacgccggg
      541 gtttgaggga ggccgccgga tttccttcgg tttttccgct ctttttttgg attgaaactt
      601 tggggccggg acgcgcgtct tatcctcgaa gctgttgaga acgatccacg caaaaggagc
      661 agccatgaaa aagacccaag ccgttctagc cgccctgttg gccctgtcct tcgcctcggt
      721 ctccctggcc gaggaccatc ccatgagccc caaacaccgt ttggtgcgcc aacacgcccg
      781 catcgaggcg ggtgtgaaga acggcagcgt cacgccggcg gaacataaga agctggccaa
      841 ggaaggccgc cacatcaacc gggaacgccg ccgggacctc aaaaaagacg ggggcaagtt
      901 gacgccggcc gataaagcca agttggagcg ccaggagaac gtccgctcgg cccagatcgc
      961 caaggacaag cacaactaag cccgcccggt cttccatccc gcctccgggt ccccgggggc
     1021 gggatttttt atttgccccc aaacctgata aactaccccc ctcatggaac accgtctcca
     1081 cctctactac ggctatggca agggc
//
LOCUS       DASTJJ010000331         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1759189, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000331 DASTJJ010000000
VERSION     DASTJJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759189"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..833
                     /gene="leuD"
                     /locus_tag="VFR02_04165"
     CDS             231..833
                     /gene="leuD"
                     /locus_tag="VFR02_04165"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9869679.1"
                     /translation="MSKIDQIEGRGVPVEGNDIDTDRIIPARYLKEITFTNMGKYPFY
                     DERFDAEGKPKGHPFDLPQFAGARLLFVNQNFGCGSSREHAPQSLMRWGVRAIVGESF
                     AEIFAGNCTMLGIPTVTVTQAQAQKLQALVKENPQAHFTLDLAKKTLLADGKEHLKFE
                     IHESKRNALVNGTWDSTGLLLANAGKTAAVAAKLPYVGGF"
BASE COUNT          202 a          333 c          327 g          160 t
ORIGIN      
        1 agggctcgaa ggacggccgc acgctgttga tgagccccgc catggtggcg gccgccgccg
       61 tcacgggcca agtgaccgac gtgcgggaac tgctgaagag cgtttgaacc accatgccgc
      121 tccgcggcac acgaaggaca cgaagttaag accgaaattt tttgatccta cactttgtga
      181 cctttgtggc cctgaagggg ctcgtggtaa aaaatcctga ggagactgtc atgagcaaga
      241 tcgatcagat cgagggccgc ggggtgcccg tggagggcaa cgacatcgac acggaccgca
      301 tcatcccggc ccgttacctg aaggagatca ccttcaccaa catgggcaaa taccccttct
      361 acgacgagcg cttcgacgcg gaaggcaagc ccaagggcca tcccttcgac ctgccccagt
      421 tcgccggggc ccggctgctc ttcgtgaacc agaacttcgg ctgcgggtcc tcgcgcgagc
      481 acgcgcccca gtcgctcatg cgctggggcg tgcgggccat cgtgggtgag tccttcgcgg
      541 agatcttcgc gggcaactgc accatgctgg gcatccccac ggtcaccgtc acccaggccc
      601 aggcgcaaaa actgcaggcc ctggtgaagg agaaccccca ggcccacttc accctggacc
      661 tggcaaagaa gacgctcctg gccgacggga aggaacactt gaaattcgag atccacgagt
      721 ccaagcgcaa cgccctggtg aacggcacct gggattccac gggcctgctc ctggccaacg
      781 cgggcaagac cgccgcggtg gcggccaagc tgccctatgt gggcgggttt taggcccggg
      841 aagttccgtg gccctttgag cccggccccc tcatgagcga actggtctgc ttggagtgtg
      901 ggaaggaaat ggaatacgac tgggccccct gtccccattg cggctggaag gcgcccggcc
      961 cctgggaaga gccggatgag gaggacgagg cgcccgcccc ccgggccgtc ctggccaagc
     1021 cc
//
LOCUS       DASTJJ010000332         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_292455, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000332 DASTJJ010000000
VERSION     DASTJJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292455"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFR02_04170"
     CDS             <1..860
                     /locus_tag="VFR02_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869680.1"
                     /translation="MPIRPKFLAGLLLLAAAPTQAAVKDQPVPYQAAVLEDQGLVHEL
                     QRPTYAFRVELVDDGQGPIPESSRGGKPFVAAVAGERYSVRLTNPLPVRVAVNLTVDG
                     LNSISGKPCGPADGKKWLIEPYGSILIPGWQVSGGEARRFFFTPKHRSYAQWRGDRTG
                     RDLAANCGVIGAAYFWSQADLDRYGEEHPLDRTTRRPLSCASSHACGLGAPACAPRAP
                     EQAMDKKDADEPAGTGMGERESHPTEEVAFDYDRGMYSPAQAVVIYYDFAKPQFPQAF
                     LDGGFAPQP"
     gene            925..>1390
                     /locus_tag="VFR02_04175"
     CDS             925..>1390
                     /locus_tag="VFR02_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869681.1"
                     /translation="MDGKGPQGQWLDAFLPAWRLRQADRIAVAAPPDKAWAVVRAIDL
                     YRFGFVRSLFGLRTWPDRLGGRGGALPKSMTLDDIAAGPAPGFRILREEPGREIVLGA
                     IGQVWQARIPFREVDAEDWAAFQEPGYVKVAWNLSAGPGPQGGTILGVEVRVG"
BASE COUNT          211 a          501 c          470 g          208 t
ORIGIN      
        1 ccatgcctat ccgaccgaag ttcctggcgg gcctgttgtt gttggccgcc gccccaaccc
       61 aagccgccgt gaaggaccaa ccggtcccct atcaagccgc cgtcctggag gaccagggcc
      121 tggtccatga gctgcagcgg cccacctatg cctttcgggt ggaactggtc gacgacggcc
      181 agggcccgat ccccgaatcc tcgcggggcg gcaagccctt cgtggccgcc gtggcgggcg
      241 aacgctattc ggtgcggctc accaaccccc tgccggtgcg ggtggcggtc aacctgacgg
      301 tggacgggct caactccatc tcgggaaaac cctgcgggcc cgccgacggg aaaaagtggc
      361 tgatcgaacc ctatgggtcc atcctcatcc ccggttggca ggtgagcggc ggcgaggccc
      421 ggcgcttttt cttcacgccc aagcaccgtt cctacgccca atggcgcggc gaccgcaccg
      481 ggcgggacct ggccgccaat tgcggcgtca tcggcgcggc ctatttctgg agccaggccg
      541 acctggaccg ctacggggaa gaacaccccc tcgaccgcac cacccggcgc cccctttcct
      601 gtgcgagctc ccacgcctgc gggctcggcg cccccgcctg cgcgccccgg gccccggaac
      661 aggccatgga caagaaggac gccgacgaac cggccggcac gggcatgggt gaacgggaat
      721 cccaccccac cgaggaagtg gcctttgatt acgaccgggg catgtattcc cccgcccagg
      781 cggtggtcat ttactacgac ttcgccaagc cccagttccc ccaggccttt ctggacgggg
      841 gcttcgcccc ccagccctga ggcctccggc cgctctatgc gcccgctcat acaacggccg
      901 atccggcggc gcgaccatgg aggcatggat ggaaaaggac ctcaaggaca atggctggac
      961 gccttcctgc ccgcttggcg cctgcgccaa gcggaccgga tcgccgtggc cgcgccgccc
     1021 gacaaggcct gggcggtggt ccgggccatc gacctctacc gtttcggttt cgtgcggtcc
     1081 ctcttcgggc ttcggacctg gccggaccgc ctgggcggcc ggggcggcgc cttgcccaaa
     1141 agcatgacgc tcgacgacat cgcggccggg ccggcgccgg gcttccggat cctccgggaa
     1201 gaaccgggac gggagatcgt gctgggcgcc atcggccaag tttggcaagc ccggatccct
     1261 ttccgggagg tggacgcgga ggattgggcc gccttccagg agccgggcta tgtgaaggtg
     1321 gcctggaacc tcagcgcggg gcccggcccc caaggcggca cgatcctcgg ggtggaggtg
     1381 cgggtgggcg
//
LOCUS       DASTJJ010000333         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_903870, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000333 DASTJJ010000000
VERSION     DASTJJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903870"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /gene="trpA"
                     /locus_tag="VFR02_04180"
     CDS             complement(<1..675)
                     /gene="trpA"
                     /locus_tag="VFR02_04180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9869682.1"
                     /translation="MSQALRLLFQEARRAGRALFLPYVCVGYPSYAASLKTAEAALKA
                     GAAGLELGVPFSDPIADGPTLQMATQCALDHGAHVAQVFRLIRDLRRRGFGQPLLVMT
                     YLNPVEQMGWGTFARRIARAGGDGAIVPDLPLERFADGGAALGAFGLSLIPFLAPTSS
                     PDRVRKVEALQAPFLYYVSLTGVTGARRSLAPGLLAALRELRKKLRTPVVVGFGISNA
                     RQAAQVG"
     gene            complement(672..1886)
                     /gene="trpB"
                     /locus_tag="VFR02_04185"
     CDS             complement(672..1886)
                     /gene="trpB"
                     /locus_tag="VFR02_04185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9869683.1"
                     /translation="MSPKQPHHLPDAAGYFGPYGGQYVPETLMVALRELSDAYQAARR
                     DPGFQAELRRYLREYSGRPTPLTYAENLTRHLKGARIFLKREDLNHTGSHKINNCLGQ
                     ILLAKRMGKKRVIAETGAGQHGVATATVCALFGLQCEVFMGEVDVERQALNVFRMRLL
                     GAKVHAVKSGTRTLKDAMSEALRDWVTRVKTTYYLIGSAAGPHPYPEMVRDFQKVLGE
                     EARVQIKRATGGLPDALVACVNGGSNAIGLFYPFLKDKKVKLYGVEAAGDGVNSGRTA
                     ATMAKGRPGILHGSHSYVMQTKEGQIAETHSISAGLDYPGVGPEHSYLRDQGRASYVA
                     VTDAQALEAFQALARHEGILAALESSHAVHYAMRLAPRMPKKASLIVNLSGRGDKDVN
                     TVLQRLGDKGRP"
     gene            complement(1977..>2266)
                     /locus_tag="VFR02_04190"
     CDS             complement(1977..>2266)
                     /locus_tag="VFR02_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869684.1"
                     /translation="QDVEKAVLLYKDILLKLPEALEALINLRDLYQRHNQADLAVECS
                     LRLGDLYNQQDYVDKAEAEYRNALALAPDHAEAREKLEKLKNEASGFPTAS"
BASE COUNT          355 a          750 c          790 g          371 t
ORIGIN      
        1 ccccacctgg gccgcctggc gggcgttgga aatgccgaag cccaccacca ccggcgtgcg
       61 cagctttttc ctgagttcac gcagcgcggc caaaagaccc ggcgccaggg accggcgcgc
      121 gccggtcacc ccggtgaggg aaacataata aaggaagggc gcctgcagcg cctccacctt
      181 ccggacccgg tccgggctgg acgtgggggc caggaagggg atgagcgaga gcccgaaagc
      241 gcccaacgcg gcgcccccgt cggcgaaacg ctccaacggc aggtccggca cgatggcccc
      301 gtcccctccc gcccgggcga tccgccgggc gaaggtcccc caccccatct gttccaccgg
      361 gttcaggtag gtcatgacca acaggggctg cccgaacccc cggcggcgca ggtcccggat
      421 gagccggaag acctgggcca catgggcccc atggtccagg gcgcattggg tcgccatctg
      481 cagggtgggg ccgtcggcaa tggggtcgga aaagggcaca cccagctcca atccggcggc
      541 cccggccttc aacgccgcct cggcggtctt gagcgaggcc gcgtagcttg gataacccac
      601 acagacgtag ggaaggaaca aggcccggcc cgcgcggcgg gcttcctgga aaagaaggcg
      661 cagggcttgg ctcacggacg ccccttgtcc ccgagccgtt gcaggaccgt gttcacgtcc
      721 ttgtcgcccc ggcccgacag gttcacgatc agggaggctt tcttgggcat gcgcggggcc
      781 aaccgcatgg cgtaatggac ggcgtgggag ctttccaagg cggccaaaat gccctcatgc
      841 cgggccaggg cctggaaggc ctccagcgcc tgggcgtcgg tcaccgccac gtaggaagcg
      901 cggccctggt cgcgcaggta gctgtgctcc ggccccaccc cggggtagtc cagcccggcg
      961 gagatggaat gggtctcggc gatctggcct tccttcgtct gcatgacgta gctgtggctg
     1021 ccgtgcagga tcccggggcg ccccttggcc atggtggccg ccgtccggcc gctgttcacc
     1081 ccgtcgcccg ccgcctccac gccgtagagc ttcaccttct tgtccttcaa gaagggataa
     1141 aaaaggccga tggcgttgga gccgccgttc acgcaggcca ccaaggcgtc gggcagtccg
     1201 ccggtggcgc gcttgatctg gacccgcgct tcctcgccca ggaccttctg gaaatcccgg
     1261 accatctccg gataggggtg ggggcccgcg gcggaaccga tgagatagta ggtggtcttg
     1321 acccgggtca cccagtcgcg cagggcctcg ctcatggcgt ccttcagggt gcgggtgccc
     1381 gacttgaccg cgtgcacctt ggcgcccagc agcctcatgc ggaagacgtt cagggcctgg
     1441 cgttccacgt ccacctcgcc catgaagacc tcgcactgga gcccgaagag cgcgcagacc
     1501 gtggcggtcg ccacgccgtg ctggccggcg ccggtctcgg cgatgacgcg tttcttgccc
     1561 atgcgcttgg ccagcaggat ctggcccagg cagttgttga tcttgtgcga gccggtgtgg
     1621 ttcaggtcct cacgcttgag gaatatccgc gcgcccttca ggtggcgcgt gaggttctcg
     1681 gcgtaggtga gcggggtggg gcggccgctg tattcccgca ggtaacggcg cagttccgcc
     1741 tggaagccgg ggtcccggcg cgcggcctgg taggcgtcgg acagttcccg cagggccacc
     1801 atcagggtct cggggacgta ctggccgcca taggggccga aatagccggc cgcgtcggga
     1861 agatggtggg gttgttttgg ggacaaacgg atcctccagg gaccggcgaa ggtcccgata
     1921 gggtaaccgc agcccctttg aaaagcaaaa aaattccggc cccgacgggg cccgggtcag
     1981 gaggcggtgg ggaacccgct ggcttcgttc ttgagttttt ccagcttctc ccgggcctcc
     2041 gcgtggtcgg gcgccagggc cagggcgttg cggtactcgg cctccgcctt gtcgacataa
     2101 tcctgttggt tgtaaaggtc gccgaggcgc aggctgcact ccaccgccag gtccgcctgg
     2161 ttgtggcgtt ggtagaggtc ccgcaggttg atcagggcct ccagggcctc gggaagcttc
     2221 aagaggatgt ccttgtagag caggaccgcc ttttccacgt cctggg
//
LOCUS       DASTJJ010000334         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_780142, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000334 DASTJJ010000000
VERSION     DASTJJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780142"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFR02_04195"
     CDS             <1..692
                     /locus_tag="VFR02_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869685.1"
                     /translation="PQNMDDLEKGIPGVHFLRYSAYKDPMNKSDGSWRFIYVNAAGAL
                     VGSVRYATLQQMAILDLNHGQWPAPAGSDSGQDAGSGQTGQGQPGASNSRAGGQQGGM
                     PTGPALAAMGQLKPTGAVDGPVIGGFLTGVASKVDHASLKVYGGGTRYNQWEFIWNPI
                     EDQMRALQQAQSQMGGTGIMGGGLGGTQPGAGGMNLNFHLNGSPGGTPVGGSGHAGGP
                     PASRPMPPPMH"
BASE COUNT          196 a          360 c          308 g          187 t
ORIGIN      
        1 acccccagaa catggacgac ctcgaaaagg gcattccggg cgtgcatttc ctgcgctatt
       61 cggcttacaa ggacccgatg aacaagtccg acggctcctg gcgctttatc tacgtcaacg
      121 ccgccggggc gctggtcggc agcgtgcgct acgccaccct ccagcagatg gccatcctcg
      181 acctgaacca cggccaatgg cccgcccccg ccggctccga ctcagggcag gacgccggct
      241 cgggccagac cggccagggc cagccgggtg cttccaactc gcgcgcaggc ggccaacagg
      301 gcgggatgcc caccgggcca gccctcgcgg ccatgggcca gcttaagccc accggcgcgg
      361 tggatggccc cgtgatcggc ggcttcctca ccggcgtcgc cagcaaggtg gaccacgcct
      421 cgctcaaggt ctacggcggc ggtacgcgct ataaccaatg ggaattcatc tggaatccca
      481 tcgaggacca aatgcgcgcc ctccagcagg cgcagagtca aatgggcggc acaggcatta
      541 tgggaggcgg cctaggcggc acgcagcctg gcgcgggcgg catgaacctc aatttccatc
      601 tcaacgggtc gcctggcggc acacccgtcg gcggctccgg ccacgccgga ggcccgcccg
      661 cgtctcggcc aatgccgccc ccgatgcatt agctgcgctc ctaagtggct ggcttcaggc
      721 cctccacgcg acacctaata cctgtacttt cgtacagtta cacgttacaa gaccactgcc
      781 tccctcccgg attgacccct ttcgccccct gccttaacgt tgttccaacg caagatttcg
      841 ggtaaacgtg ggcggttaga aggcgcgcac tggggtctca taagtgcctt cgcgtgatcc
      901 cggggtacaa atcctttaaa gagaaaaatg gaggacggta atgaagaaat tgatgtggtt
      961 ggccctcgct atatcgctct ttaccctgcc ttcgttcgcg caggaagtgc cgtccatgag
     1021 cctttcgggc ggctattcct atttccgcat c
//
LOCUS       DASTJJ010000335         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_780252, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000335 DASTJJ010000000
VERSION     DASTJJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780252"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFR02_04200"
     CDS             <1..1345
                     /locus_tag="VFR02_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9869686.1"
                     /translation="VPTNMAAVKKAYEAVDALPDEMHEQFPYHLEPKKNTPKRLLIAG
                     AQATALGKLKAGCRYQTYYPISPATDESNYLESHPEYGIAVVQCEDEIAAIDMALGAA
                     TTGVRASTATSGPGFSLMTEGLGWGGMNEVPIVVFNYQRGGPSTGLPTRHEQGDLLFS
                     VFGGHGEYPRLVMAPATIDDHFHLAFEAFNYADKFQTPVVILNDKFLTHSIQTIPPFD
                     EGNLVVDRGRLATPQELESAIADGKGFPRFKPDPKTLVSPRPYPGQEGSPYWMTGDEH
                     DDYGHITESPETRKKMHEKRMGKYKLMLEQIPEDRQYLLEGEADADVTLVCWGTTSGV
                     LRDVAGALKVKGIKANILTYRMLNPFPIATEKILKKAKKLILVESNLGTQLGALIRMR
                     TGIFIPSQIVKYTGRPMSLNEVVDSVEKFHKKKVQAEVDPLTGLSVEKVVLTYGL"
     gene            1335..>1945
                     /locus_tag="VFR02_04205"
     CDS             1335..>1945
                     /locus_tag="VFR02_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9869687.1"
                     /translation="MVSKLQAPLTVAAYKSDVHNDWCPGCGDFGILQAVQQSCAELSM
                     PPWETMVFSGIGCSGKVFNYMATHGIHTLHGRVLPFAVGAKLANPDLEVVAVGGDGDG
                     YGIGAGHFVHAGRRNVDMAYIVMTNEVYGLTKGQASPTMGRGQQTKSLPLPNTNDAVQ
                     PLALALGAGYTWIGRSYAFDGKHLKEMIKRAIEHKGMALLDVL"
BASE COUNT          401 a          603 c          606 g          335 t
ORIGIN      
        1 ggtccccacc aacatggcgg cggtcaagaa agcgtatgag gcggtggacg ccttgcccga
       61 cgagatgcac gaacagttcc cctaccacct ggaaccgaag aagaacacgc ccaagcgtct
      121 tttgatcgcc ggggcccagg cgacggcctt ggggaagctc aaggcgggtt gccgctacca
      181 aacatattac ccgatcagcc ccgccacgga cgagagcaac tacctggaaa gccatccgga
      241 atacggcatc gcggtggtcc aatgcgagga cgagatcgcg gccatcgaca tggccttggg
      301 ggcggccacg accggcgtgc gcgcgtccac ggccacctca ggcccgggtt tcagcttgat
      361 gaccgaaggc ttgggatggg gaggcatgaa cgaggtgccg atcgtggtgt tcaattacca
      421 acggggcggc ccttccacgg gtctgcctac ccggcatgag cagggggacc tgcttttttc
      481 cgtctttggg ggccacggcg aatatccccg tctggtgatg gcgccggcca ccattgatga
      541 ccacttccac ttggcctttg aggccttcaa ctacgccgac aagttccaaa ccccagtggt
      601 cattttgaac gacaagttcc tgacccacag catccagacc atcccgccct ttgacgaggg
      661 caatttggtc gtcgatcgag gccgcttggc cacccctcag gaattggaat cggccatcgc
      721 ggacggcaag ggttttccgc gtttcaagcc cgatcccaag acgctcgtct cgccccggcc
      781 ctatcccggc caggagggaa gcccctattg gatgacgggg gatgagcacg acgattacgg
      841 ccacatcacg gaaagccccg agacccggaa gaaaatgcac gagaaacgca tgggcaaata
      901 caagctcatg ctggagcaga tccccgagga ccggcaatac ctgctggaag gggaggctga
      961 cgccgacgtg accctggttt gttgggggac cacgtcgggc gtgctgcggg acgtggccgg
     1021 ggccctgaag gtcaagggca tcaaggccaa tatcctgacc taccgcatgc tgaatccttt
     1081 ccccatcgcc accgagaaga tcttgaagaa ggccaagaag ctcatcctgg tggaaagcaa
     1141 cctggggacc caactggggg cgctcatccg catgcgtacg gggatcttca tcccctcaca
     1201 gatcgtcaag tacacgggac gccccatgtc gctcaatgag gtcgtggatt cggtggagaa
     1261 gttccacaag aaaaaggtcc aggccgaggt ggatccatta acgggtttgt ccgtggaaaa
     1321 ggtggtgttg acctatggtc tctaaacttc aagcgccctt gaccgtcgcg gcctataaga
     1381 gcgacgtcca caacgactgg tgccccggat gcggcgattt cggcatcctg caggcggtcc
     1441 agcagtcctg cgcggagctg tccatgccgc cctgggagac catggtcttc tccggcatcg
     1501 gttgctcggg caaggtcttc aactacatgg ccacccatgg gatccatacc ctgcacgggc
     1561 gcgtcctgcc cttcgcggtg ggggccaaac tggccaatcc ggacctggag gtcgtggccg
     1621 tgggcggcga cggcgacggg tatggcatcg gggcgggcca tttcgtgcac gccggccggc
     1681 gcaacgtgga catggcctat atcgtgatga ccaacgaggt ctatgggctc accaagggcc
     1741 aggcgtcccc cacgatgggc cgcgggcagc agaccaagtc cctgcccttg cccaacacca
     1801 acgacgccgt ccagcccctg gccctggcct tgggggcggg ctacacctgg atcggacgca
     1861 gttacgcttt cgacggcaag cacttgaagg agatgatcaa gcgggcgatc gagcacaagg
     1921 gcatggccct gctggacgtc ctcca
//
LOCUS       DASTJJ010000336         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1393549, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000336 DASTJJ010000000
VERSION     DASTJJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393549"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFR02_04210"
     CDS             <1..266
                     /locus_tag="VFR02_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9869688.1"
                     /translation="EGREFGNVPVTIEMKLEVWDSPNSAGVAIDAIRCCKLALNAGVA
                     GALEGPSSYFMKTPPLQYSDSEAKEFVEQFIAQYGKAGAKVKA"
     gene            328..1149
                     /locus_tag="VFR02_04215"
     CDS             328..1149
                     /locus_tag="VFR02_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET9869689.1"
                     /translation="MNRIFLLACLCPAVPACAWGPVGHETVAIIAQDHLTPKALRAVR
                     DLLGPGQSLASVANWADEIVDERPETAPWHHIDLPVRKDLTEADIPDYCPKGDCVVGQ
                     IERDLAVLGNPADPRPQKAEALKFLVHLVGDLHQPLHCANDDDRGGNQKHLSLHPDFS
                     RHPHWMSLHAYWDHLLEEKTRDVPRALATRLEARVTPQDAVRWQRGSVEDWAFESYRI
                     AQEDIDAAFPPGPMRPWDQVPVPRAYRDGAMRWLVDRQLEKAGLRLAWLLNRTFP"
     gene            1146..>1777
                     /locus_tag="VFR02_04220"
     CDS             1146..>1777
                     /locus_tag="VFR02_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9869690.1"
                     /translation="MKPPTLRADTAIEKATVYTGASAKPLKEATVLITGETLAYVGPH
                     AKRPDYQAKETIDGRGMTLLPGLIDLHVHALINEATLASFLANGVTAIRDLASDPFHA
                     LEWKAREQTGLIASPRIFTSGPVLTCPHGYPENVWGPQLALKLTGRYQAEEKVRQLAG
                     LGMDLIKLGLEHELGPCLSQTEVEAAVDAAHSLGKRVTAHITDARDFESCV"
BASE COUNT          295 a          636 c          583 g          263 t
ORIGIN      
        1 tggagggccg cgagttcggc aacgtgcccg tcaccatcga gatgaagctg gaggtctggg
       61 attcgcccaa cagcgccggg gtggccatcg acgccatccg ctgctgcaag ctggccttga
      121 acgccggggt ggccggcgcc ctggagggcc cttcctctta tttcatgaag acgccccccc
      181 tgcagtattc ggactcggag gccaaggaat tcgtggaaca gttcatcgcc cagtacggca
      241 aggcgggggc gaaggtcaag gcctgatccc cgaccccatt gcttcccggg cccgggggtt
      301 ccctgggccc ttttcatttg aggcgccatg aaccgcatct tccttcttgc ctgcctttgc
      361 cccgcggtcc cggcttgcgc ctggggcccg gtgggccatg agaccgtggc catcatcgcc
      421 caggaccatt tgaccccgaa ggccctgcgg gccgtgcggg acctattggg cccgggccaa
      481 agcctggcct cggtggccaa ttgggcggat gagatcgtgg acgaacggcc cgagaccgcc
      541 ccctggcacc atatcgacct tccggtgcgc aaggacctca cggaggccga catcccggat
      601 tattgcccga agggggactg cgtggtgggc cagatcgagc gggacctggc cgtcctgggg
      661 aacccggcgg atccgcgccc ccaaaaggcc gaggccctga aattcctcgt ccatctggtg
      721 ggcgacctgc accagcccct gcattgcgcc aacgacgacg accggggcgg caaccaaaaa
      781 cacctgtccc tgcacccgga cttcagccgg cacccccatt ggatgagcct gcacgcctat
      841 tgggaccacc tgctggagga aaagacccgg gacgtgcccc gggccctggc cacccggctg
      901 gaagcccggg tcaccccgca ggacgcggtc cgctggcagc gggggagcgt ggaggactgg
      961 gccttcgaga gctaccgcat cgcccaggag gacatcgacg cggcgttccc gccgggcccc
     1021 atgcggccct gggaccaggt gcccgtgccg cgggcctacc gcgacggcgc catgcggtgg
     1081 ctggtggacc gccaactgga gaaggcgggc ttgcggctgg cctggctcct gaaccggacc
     1141 ttcccatgaa gccccccacc ctgcgggcgg acaccgccat cgagaaggcc acggtctaca
     1201 cgggcgcttc cgccaagccc ttgaaggaag ccacggtcct catcacgggc gagaccctgg
     1261 cctacgtggg cccccacgcc aagcggcccg actaccaggc gaaggaaacg atcgacggcc
     1321 ggggcatgac cctgctgccg ggcctcatcg acctgcacgt gcacgcgctc atcaacgagg
     1381 ccaccctggc cagtttcctg gccaacggcg tgacggccat ccgggacctg gcctccgacc
     1441 ccttccacgc gctcgaatgg aaggcccgcg agcagaccgg gctcatcgcc tcgccccgca
     1501 tcttcacctc ggggccggtg ctcacctgcc cccacggtta tccggagaac gtctgggggc
     1561 cccaactggc gctcaagctc accggccggt accaggccga ggagaaggtg cgccaactgg
     1621 ccgggctggg catggacctc atcaagctgg ggctggagca tgagttgggc ccctgtcttt
     1681 cccagaccga ggtggaggcc gcggtggacg ccgcccattc cctgggaaaa cgcgtgaccg
     1741 cccacatcac ggacgcgcgc gatttcgagt cctgcgt
//
LOCUS       DASTJJ010000337         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1759726, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000337 DASTJJ010000000
VERSION     DASTJJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759726"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFR02_04225"
     CDS             complement(<1..1013)
                     /locus_tag="VFR02_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9869691.1"
                     /translation="MEAYNFTDIERRWKAAWKKDRSFACDLSRADNKYYTLVMFPYPS
                     GDFLHAGHGRNYIIGDAVYRYFRALGRNVLNPMGFDAFGLPAENTAIKKGIHPEDWTL
                     KNIERMKGQFDDWGMGYDWDKEVVSCLPDYYRWTQWIFLKLHEKGLAYRKKANVNWCP
                     TDMTVLANEQVVDGRCERCDSLVEQKDLEQWFLKITDYADRLLEDLGKLENWPERVKT
                     MQRNWIGRSEGAEVEFQVEGSGETLTVFTTRPDTLFGATYMVLAPEHPLVEKLTAGTP
                     KEQAVRDFASAARKMSSLDRLGGAGEKLGLDTGRKAVNPVNGKAVPIFIANYVLMGYG
                     TGA"
     gene            complement(1087..>1423)
                     /locus_tag="VFR02_04230"
     CDS             complement(1087..>1423)
                     /locus_tag="VFR02_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869692.1"
                     /translation="LFGWTIDPPASLAYWFLGGMLFFTSGACLLGGSRSTVWVVPAVH
                     LFFCADWLRGEGTWQALLQTAVEGSPRLAEALAAWEKAPGPGFPELFCLVSLFQLGVV
                     AFSRRHWTH"
BASE COUNT          234 a          464 c          472 g          253 t
ORIGIN      
        1 atggcgcccg tgccgtatcc catgagcacg tagttggcga tgaagatggg gacggccttc
       61 ccgttgaccg ggttgacggc cttgcgcccg gtgtccaggc ccagtttttc cccggccccg
      121 cccaggcggt ccagcgagct catcttgcgg gcggccgacg cgaagtcccg gaccgcctgt
      181 tccttcgggg tgccggcggt gagcttttcc accaggggat gctcgggggc cagcaccata
      241 taggtggccc cgaagagggt atcgggccgg gtggtgaaga ccgtcagggt ctccccgctc
      301 ccctccacct ggaactccac ctcggcgccc tcgctgcggc cgatccagtt gcgctgcatg
      361 gtcttgaccc gctcgggcca gttttccagc ttgcccaggt cctccagcag ccggtcggcg
      421 tagtcggtga tcttgaggaa ccactgttcc aggtccttct gttccaccag cgagtcgcag
      481 cgctcgcagc ggccgtccac cacctgttcg ttggccagga ccgtcatgtc ggtcgggcac
      541 cagttcacgt tggccttctt gcggtaggcc aggcccttct cgtggagctt caggaagatc
      601 cattgggtcc agcggtagta gtcgggcagg caggacacca cttccttgtc ccaatcgtag
      661 cccatgcccc agtcgtcgaa ctggcccttc atgcgctcga tgttcttgag ggtccagtcc
      721 tcgggatgga tgcccttctt gatcgcggtg ttctccgcgg gcaggccgaa ggcgtcgaag
      781 cccatggggt tgagcacgtt gcggcccagg gcccggaaat agcggtacac ggcgtcgccg
      841 atgatgtagt tgcggccgtg gccggcgtgc aggaagtcgc ccgagggata ggggaacatg
      901 accagggtgt aatacttgtt gtcggcgcgg gaaaggtcgc aggcgaagct gcggtccttc
      961 ttccacgcgg ccttccagcg ccgttcgatg tcggtgaaat tgtaggcttc cattccctgt
     1021 ccttttcccg gaccggccgg tccgggccaa aattatagac gattcaaggc aaaagccaaa
     1081 gccgggctag tgcgtccaat ggcggcgcga gaaggccacc acgcccagtt ggaagaggct
     1141 gaccaggcag aagagctcgg ggaaacccgg ccccggcgcc ttttcccagg cggccaaggc
     1201 ctccgccagc cggggcgacc cttccacggc ggtctgcagc agggcctgcc aggtcccctc
     1261 gccgcgcagc cagtcggcgc agaagaacag gtgcacggcc ggcaccaccc agaccgtgct
     1321 gcgggaaccg cccaacaggc aggccccgct ggtgaagaag agcatgccgc ccaggaacca
     1381 gtaggccagg gaggccggcg ggtcgatggt ccagccgaag agc
//
LOCUS       DASTJJ010000338         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1026831, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000338 DASTJJ010000000
VERSION     DASTJJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026831"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1526
                     /locus_tag="VFR02_04235"
     CDS             <1..>1526
                     /locus_tag="VFR02_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HET9869693.1"
                     /translation="AFSNLFIRTEFLPELSALTAGRRPRSPSEGPAYMAQVVSHEGPV
                     LGDIEYETDRAQFIGRGGSLARPRAVQGGRPLANRVGDVLDPILSLRVRVRLAPGASA
                     KVCFTTLASDSRMEMLALAEKYHNASAFERASGLVWTQAAAALYHLGIDPEEAQLFQR
                     LANRILFSDPLLRPTGEILRKNRLDRTGLWSRGISGDLPILLVRIDDLEDPTLIRQLL
                     RAHDYWRLKRLAVDIVIVNEKAASYTQELQTALEGMVRGSSDPARGGPDPSPGKFFVL
                     RGDLLDPREKELLAAVARAILSNRSGTLAEQVLRVRRSGGFAPTQGRTLAEMEAARLR
                     DRTEPPLAVPPLEFFNGVGGFADGGREYAVVLGKGRRTPAPWINVVANPGFGFQVSES
                     GAGYTWAVNSRENQITPWSNDPVLDPPGEAFFLGDLDTGALWSPTASPIRLEGASYLA
                     RHGAGYSVFENGSNGIHSTLTQFVDPEAPVKISRLVLENTSGRARRLSVTGYVEWVLG
                     "
BASE COUNT          227 a          537 c          506 g          256 t
ORIGIN      
        1 cgccttctcg aaccttttca tccgaaccga attcctcccc gaactgtccg ccttgacggc
       61 cggccggcgt ccccggtccc cctccgaggg gcccgcctac atggcccaag tggtctccca
      121 cgaagggccc gtcctgggag acattgaata cgagaccgat cgggcccaat tcatcggccg
      181 ggggggatcc ttggccaggc cgcgggcggt ccaggggggc cgccccctcg ccaaccgggt
      241 cggggacgtg ctggatccca tcctgagcct ccgggtcagg gtccgcctcg cccccggggc
      301 gtcggccaag gtttgtttca ccaccctggc gtccgattcg aggatggaaa tgttggccct
      361 ggccgaaaaa taccataatg cgtccgcttt cgagcgcgcg tcgggcctgg tctggaccca
      421 agcggccgcc gccctctacc acctgggcat cgaccccgag gaggcccaac tgttccaacg
      481 gttggccaac cgcatccttt tttccgaccc gctgctgcgg ccgacgggcg agatcctgcg
      541 aaagaaccgg ctggaccgga ccggcctctg gtcccggggc atctcggggg acctgcccat
      601 cctgttggtc aggatcgacg acctggagga cccgaccctc atccgccagt tgctccgggc
      661 ccatgattat tggcgcttga agcgcctggc ggtggacatc gtgatcgtga acgaaaaggc
      721 ggcctcctac acgcaggagc tccagaccgc cttggagggg atggtacgtg gaagttcgga
      781 ccccgcgcgg ggcggtccgg acccgtcccc cgggaaattt ttcgtcctgc ggggcgacct
      841 gttggacccc cgggaaaagg aactcttggc ggccgtcgcc cgggccatcc tcagcaaccg
      901 gtcgggcacg ctggccgagc aggtcctgcg ggtccgtcga tccggcggct tcgccccgac
      961 ccagggccgg acgctcgcgg agatggaggc cgcccggctg cgggaccgga ccgagccccc
     1021 gctggccgtg cctcccctcg aatttttcaa cggtgtgggc gggttcgcgg acggggggcg
     1081 ggaatacgcg gtggttttgg ggaaaggccg gcggacaccg gccccctgga tcaacgtggt
     1141 ggccaatccc ggtttcggtt tccaggtctc cgagtcgggc gccggctaca cctgggcggt
     1201 caacagccgg gaaaaccaga tcaccccctg gtccaacgac cccgtcctcg accctcccgg
     1261 ggaggccttc ttcctgggcg acctggacac gggcgccctt tggtccccca cggcgtcgcc
     1321 catccggttg gagggggcct cctacctggc gcggcacggc gccggttaca gcgttttcga
     1381 gaacggatcc aacggcatcc acagcaccct gacccaattc gtggacccgg aggcgcccgt
     1441 caagatctcc cgcctggtcc ttgagaacac ctcgggccgg gcgcgccgcc tgtcggtgac
     1501 gggttacgtg gaatgggtcc tgggtt
//
LOCUS       DASTJJ010000339         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_293034, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000339 DASTJJ010000000
VERSION     DASTJJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293034"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1430
                     /locus_tag="VFR02_04240"
     CDS             <1..>1430
                     /locus_tag="VFR02_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9869694.1"
                     /translation="GNARVEVFSNTGQYESQWAGFSSPSGVALDGAGNVYVSDDGNET
                     VDVFTPQGAPLRSWDTVYGVVGAGAIAVDPASGDVYIADDFGEVGLFTNTGSFLGLAQ
                     GPEGGFASLKGIAVGSGVWAAADPGQGMVDLFQACSRTPLPTPTLTPTETRSPTETPT
                     PTLSPTITLSPTETLSPTVTPTPQPYVSCPIQGMLAADDPQGLALDPSGDLYVADYSA
                     GLIRVFDPQGDQSATLGGPSLLTFPFAVAADGTGKVYVTELGNDQIDVLDSQGGLLRQ
                     WSLTAGTDSQPAYPLGVAVDAAGATVYVSDAANQEIKYYDAQGNLLGAWGGDGTFNDL
                     SGIALDGMGRVYAADGYTGLVHVFDGLGHPIAQWNATGLSAAYFIAVDPSGDRVYLTD
                     GYGSMGVFGTDGTVIGATVPDPLVPGARGVAVGVGVVYVANNQVGEVDVLGPCMTSPA
                     PTPTPMVNPGLVCAQAASWSVNFVGGL"
BASE COUNT          218 a          461 c          484 g          267 t
ORIGIN      
        1 gggaacgcgc gggtggaggt cttcagcaac acgggccaat atgaatccca atgggcaggg
       61 ttcagcagtc cttcgggtgt ggccctggat ggggcgggga atgtctatgt ctcggacgac
      121 ggcaacgaga ccgtggacgt cttcacaccc cagggcgctc cgcttcggtc ctgggacacc
      181 gtctacggcg tggtcggcgc cggtgccatc gcggtcgacc cggcgtcggg ggacgtctac
      241 atcgcggatg atttcggcga agtggggctc ttcaccaata ccggttcctt cctgggcctg
      301 gcccagggtc cggaaggggg cttcgcttcc ctgaagggta ttgccgtggg atccggggtt
      361 tgggcagcgg ccgacccggg acaagggatg gtcgatctgt tccaagcctg ttcccggacg
      421 cccctgccga ccccgacctt gaccccgacg gaaaccaggt cccccaccga gacccccacg
      481 cccacccttt caccgaccat aaccctctcg cccacggaaa ccctctcccc gacggtgacc
      541 cccaccccgc agccctatgt ttcctgcccc atccagggaa tgctggcggc cgacgacccg
      601 caagggctgg ccctggatcc ttccggcgat ctctacgtcg ccgactactc ggccgggttg
      661 atccgggtct tcgaccccca gggcgaccag tccgcgaccc tgggcggacc ttcgctcttg
      721 acctttcctt tcgcggtggc ggcggatggg accggtaagg tctatgtcac agaactgggg
      781 aacgaccaga tcgatgtcct ggacagccag ggcggactgt tgcgccaatg gagtttgacc
      841 gccggcaccg acagccagcc agcctatcct ttgggtgtgg ccgtcgacgc cgcaggggcc
      901 acggtctatg tttcggacgc cgcgaaccaa gagatcaagt attacgacgc ccaagggaat
      961 ttactgggcg cctggggcgg ggacgggacc ttcaacgacc tgtcgggcat cgccctggat
     1021 gggatgggcc gggtctacgc ggcggacggt tatacggggt tggtccatgt gttcgacggg
     1081 ctgggccatc cgatcgccca atggaacgcg accggcttgt ccgccgccta tttcatcgcc
     1141 gttgatcctt cgggtgaccg ggtctatttg acggatgggt atgggtccat gggggtcttc
     1201 gggaccgacg ggacggtgat cggggccacg gtgccggatc ccctggttcc gggcgcccgg
     1261 ggggtggccg tcggcgtggg agtggtctac gtggccaaca accaggtcgg tgaagtggac
     1321 gtcttggggc cctgcatgac gtcgcccgcg cccactccca cgccgatggt gaaccccggc
     1381 ttggtctgcg cccaggcggc ttcctggtcc gtgaatttcg tgggtgggct
//
LOCUS       DASTJJ010000340         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_780611, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000340 DASTJJ010000000
VERSION     DASTJJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780611"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1840)
                     /locus_tag="VFR02_04245"
     CDS             complement(47..1840)
                     /locus_tag="VFR02_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9869695.1"
                     /translation="MHFPTLLLLGFIAGGTILIGLPVGRLKGFHPTLRASLSMFAAGI
                     LVFLLVEILGEACGQTAAALAGPPAPALALTLLLMAGFLFGFVGLVGIEQKLIRSGDP
                     LDPGNLTLMIAVGIGLHNLSEGLAIGQAYAQGLSGLTLSLILGFALHNATEGFGIVGP
                     MVRAGQMVSWGRLLLLALIGGGPTFVGTLLGSLWTSVYLSVLVLAMAGGALLYVLKEL
                     LHGARRESAQALIMGVLVLGFTVGWATEVVADRSQGGGAVEADGDRISPSALGGPSLS
                     AADAAAQDQVSNDILYERAMVPKVLPDGTKEYDLTASEFTWQVFPGARVRAWGYNHQV
                     PGPLLRWKVGDKVKMVVRNELPQETTVHWHGLAVPNAMDGVPDITQKPIPPGGEFTYA
                     FTVTAQMAGTHLYHTHVNDDFQMDKGLHGVLIVDPAKNAPHPDVDALYEMASFKVGGS
                     EGENVFTLDGKACPEAPVLRVSKDQRVRIRMVNSSAEESHVMHLHGYTFWVVALDGNP
                     LAHPYAANTVNLGPSQTADVEFKADNPGLWMFHCHILDHLVNPSPAGDGSATQIPDMG
                     GLMTYVEVVSWPHPQAIYRAAGSVSGNGTCR"
     gene            complement(2093..>2290)
                     /locus_tag="VFR02_04250"
     CDS             complement(2093..>2290)
                     /locus_tag="VFR02_04250"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET9869696.1"
                     /translation="LRSMCPCAQCVDELTGRRTITLEAVDPKVRLVGVHPVGRYALNF
                     QWSDGHGTGLYTFKNLLQLGE"
BASE COUNT          389 a          715 c          825 g          361 t
ORIGIN      
        1 gcgtgaaagg cgggcccttt tcgccgggat agtgtccggc cgggccctag cggcaggtgc
       61 cgttgcctga gaccgacccg gcggcccggt agatcgcttg gggatggggc caggacacca
      121 cttccacgta ggtcatgagc ccccccatgt cggggatctg ggtggccgaa ccgtcgcccg
      181 cggggctggg gttgaccagg tggtccagga tgtggcaatg gaacatccac aaccccgggt
      241 tgtcggcctt gaactccacg tcggcggtct gggaagggcc caggttgacc gtgttggccg
      301 cgtaaggatg ggcgaggggg ttgccgtcca gggccaccac ccagaaggtg tagccgtgca
      361 ggtgcatgac gtggctttcc tcggcgctgg aattgaccat gcggatgcgc acccgctggt
      421 ccttgctgac ccgaaggacc ggcgcctcgg ggcaggcctt gccgtccagg gtgaagacat
      481 tttccccctc cgaaccgccg accttgaagg aggccatctc gtagagggcg tcgacgtcgg
      541 ggtggggcgc gttcttcgcc gggtccacga tcaggacccc gtgcaggccc ttgtccatct
      601 ggaagtcgtc attgacgtgg gtgtggtaga ggtgggtgcc cgccatctgg gcggtgaccg
      661 tgaaggcgta ggtgaactcg ccgcccgggg ggatgggctt ctgggtgatg tcgggcacgc
      721 cgtccatggc gttgggcacg gccaggccgt gccagtgcac ggtggtctcc tggggcaatt
      781 cgttcctcac caccatcttg accttgtcgc ccaccttcca ccgcagcagg gggccgggca
      841 cctggtggtt gtagccccag gcgcgcaccc gcgccccggg gaagacctgc caggtgaact
      901 cggaggcggt caggtcgtat tccttcgtgc cgtcggggag gaccttgggg accatggccc
      961 tctcatagag gatgtcgttg gagacctggt cctgggccgc cgcgtccgcc gcggacagcg
     1021 agggcccgcc caaggcggag ggggaaatac ggtcgccgtc ggcttccacg gcgccgcccc
     1081 cctgggaacg gtcggccacc acttcggtgg cccagcccac ggtgaagccc aacaccaaca
     1141 cgcccatgat gagcgcctgg gccgattccc gccgggcgcc gtgcagcagt tccttcagga
     1201 cgtacaacag ggcgcccccc gccatggcca gcaccaatac ggacaggtag accgaggtcc
     1261 aaagcgaccc caggagcgtg cccacaaagg tcgggccgcc gccgatgagg gccaggagca
     1321 ggagacggcc ccaggacacc atttgtccgg cgcgcaccat ggggcccacg atgccgaaac
     1381 cctcggtggc gttgtgcagg gcgaagccca agatgaggct cagggtcagg ccgctgaggc
     1441 cctgggcgta ggcctggccg atggcgagcc cttcgctcaa attgtgcagc ccgatgccca
     1501 cggcgatcat cagggtgagg ttccccgggt ccagggggtc gcccgagcgg atgagctttt
     1561 gctcgatgcc caccagcccc acgaaaccga acagaaagcc cgccatgagc aggagggtca
     1621 aggccagggc gggggccggg gggcccgcca gggcggccgc ggtctggccg caggcctcgc
     1681 ccaggatctc caccaacagg aagaccagga tgcccgccgc gaacatgctg agggacgccc
     1741 gcaaggtcgg gtggaagcct ttcaggcgcc cgacgggcaa cccgatcagg atggtgccgc
     1801 cggcgatgaa gccgaggagg agcagggtgg ggaaatgcat ggtggccttt ccaccgggtc
     1861 ggggggccgc ccggtgacgt ctggagttct tttattcccg cgccaagccg ggcccccacc
     1921 ttacggcttg gggcgcgggg cttgtatgac ggggatcagg tgcgtgccgc gaggaccgat
     1981 cgaggatatg atattgaaaa ccattatcaa tttccagggc aaaaagaggt ctgggacaag
     2041 gaaggcggca aaagcgtcgg gtctgggcct gggccaggcc ggggggaacg ggttattcgc
     2101 ccaattgaag caggttcttg aaggtataga ggcccgtgcc gtggccgtcg gaccattgga
     2161 aattcagggc gtagcggccc acggggtgga cgcccaccag ccgcactttc gggtccacgg
     2221 cttccagggt gatggtgcgc cttccggtca actcgtccac gcattgggcg cagggacaca
     2281 tgctcctcag
//
LOCUS       DASTJJ010000341         1327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_904778, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000341 DASTJJ010000000
VERSION     DASTJJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904778"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR02_04255"
     CDS             <1..258
                     /locus_tag="VFR02_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9869697.1"
                     /translation="KERDVDCAFKLIPRRVLKKVKLSAGGAMISTELLARAHKAGFTF
                     VEVGVTHLPRRAGTQTGANLKVILRAFKELFKLYGRINRQA"
     gene            complement(242..>1327)
                     /locus_tag="VFR02_04260"
     CDS             complement(242..>1327)
                     /locus_tag="VFR02_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9869698.1"
                     /translation="LARIVENSRDLITVSGPDGKTTYLNEAGRRMLGIDPGEDLGAIR
                     PQDFFFEEDLPRLRELDAQEGAASRERGYWEGEIRLRNRKTQRPVPVHFSVVYIKDPN
                     TGKPLGSASIGRDITQRKKVDELARSNRELEQFAYVASHDLQEPLRMVASFVQLLAME
                     YKGKLDATADQYIQEAVGGVKRMQALILDLLAYSRLDSGGRPSQKVDCAEALRQALSD
                     LEIGLRESGATVRPGELPEVTGDFHQIVQLFQNLVANALKFRSEKPPEIQVEARREGG
                     EWLFCVRDNGIGLDPRYAEQIFEIFKRLHTRREYPGTGIGLAICKKVVARHGGRIWVE
                     SRPGEGAAFYWTLPAAGEAGGGVMPGG"
BASE COUNT          201 a          480 c          420 g          226 t
ORIGIN      
        1 aaggagcggg acgtggactg cgccttcaag ctcatccccc gccgggtgct caagaaggtc
       61 aaattgagcg cgggcggggc catgatctcc accgagctgt tggcgcgcgc ccacaaggcg
      121 ggcttcacct tcgtggaggt gggggtgacc cacctgcccc gccgggccgg cacccagacc
      181 ggcgccaacc tcaaggtgat cctgcgggcc ttcaaggagc tgttcaagct ctacggcagg
      241 atcaaccgcc aggcatgacc ccgccccccg cttcccccgc ggccggcaag gtccaataga
      301 aggccgcgcc ctccccgggc cgggattcca cccagatgcg cccgccgtgc cgggccacca
      361 ccttcttgca gatggccagg ccgatccccg tgccggggta ttcgcgccgg gtgtgcaggc
      421 gcttgaagat ctcgaagatc tgctcggcgt accggggatc caggccgatg ccgttgtccc
      481 gcacgcaaaa gagccattcc ccgccctccc ggcgggcctc cacttggatc tcggggggct
      541 tctccgacct gaacttcaac gcgttggcga ccaggttctg gaacagctgg acgatctggt
      601 ggaaatcgcc cgtcacctcc ggaagctccc ccggccggac cgtcgccccg ctctcccgaa
      661 ggccgatctc caggtccgag agggcctggc gcagcgcctc ggcgcaatcc actttctggg
      721 agggccggcc cccgctgtcc aaccgggaat aggccaggag gtccaggatc agggcctgca
      781 tgcgcttcac cccgcccacg gcctcctgga tatattggtc ggcggtcgcg tccagtttgc
      841 ccttgtactc catcgccagc agttgcacga agctggcgac catgcgcagg ggttcctgca
      901 ggtcgtggga cgccacataa gcgaactgct ccagttcccg gttggagcgc gccaattcgt
      961 ccaccttctt ccgctgggtg atgtcccggc cgatggaggc gctccccagc ggcttccccg
     1021 tgttggggtc cttgatgtag accacgctga aatgcacggg gacgggccgc tgggtcttgc
     1081 ggttcctcag gcggatctcc ccctcccaat aacccctttc ccgggaagcc gcgccctcct
     1141 gggcgtccag ctcccgcagg cgggggaggt cctcctcgaa aaagaaatcc tggggccgga
     1201 tggcccccag gtcctccccc gggtcgatcc ccagcatgcg gcggcccgcc tcgttcaggt
     1261 aggtggtctt gccgtccggg ccgctcaccg tgatcaggtc gcggctgttc tccacgatgc
     1321 gggccag
//
LOCUS       DASTJJ010000342         1224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_537643, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000342 DASTJJ010000000
VERSION     DASTJJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537643"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFR02_04265"
     CDS             <1..568
                     /locus_tag="VFR02_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869699.1"
                     /translation="CTHLLFIDADIGFEPDQAFRLLDFGADLAAAAYPTKKVDWDKAR
                     VLAQAGVKDLQSSALSYVVEFSDPRKIETRDGFAKVKYAGTGFLMVRREALLKMEGRY
                     PDLKFTGQHQAEDPYRGSAHRYALFNCFVEPETGIYLSEDYSFCRRWTDMGGEIWVDL
                     KSRLTHVGPMSFSGDLATQIQVPPPTHP"
     gene            725..>1224
                     /locus_tag="VFR02_04270"
     CDS             725..>1224
                     /locus_tag="VFR02_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869700.1"
                     /translation="MLTRNLKNLSAGLLGFLIVQTGATLAWKAYAACATAGRDGSPSL
                     SGLINTYYPPAAVTTLSSGATVIALGSASGSTVPISVGDLVLVIQVQDASISVTQTAN
                     YGAGTGTGSGATAFASTGLYEYATAASTVPTGGGSLTLTAGLSNTYHEAAASAASGQK
                     TFEVIRV"
BASE COUNT          215 a          412 c          368 g          229 t
ORIGIN      
        1 gtgcacgcac ttgcttttca tcgacgcgga catcggtttc gagcccgacc aggccttccg
       61 gctgctcgac ttcggcgccg acctggcggc cgcggcctat cccaccaaga aggtggattg
      121 ggacaaggcc cgggtcctgg cccaggccgg ggtgaaggac ctgcaatcct ccgctttgtc
      181 ctatgtggtg gaattcagcg atccccgcaa gatcgagacc cgggacggct tcgccaaggt
      241 gaaatacgcg ggaacggggt tcctgatggt gcgccgggag gcgctgctga aaatggaagg
      301 gcgctatccc gacctgaagt tcacgggcca gcaccaggcc gaggacccct accggggcag
      361 cgcccaccgt tacgccctgt tcaactgctt cgtggaaccg gagaccggca tttacctgag
      421 cgaggattac agtttttgtc gccgctggac ggacatgggc ggcgagatct gggtggacct
      481 gaagagccgc ctgacccacg tggggcccat gagcttctcc ggggatctgg ccacccaaat
      541 ccaggtgcca ccccccaccc atccctgatt ttggcttttt tattgggttt tgcccctcat
      601 ttttcactcg ccaggggcaa agtcctggct tattttaggt aaacgaaaaa cctaatggta
      661 agtggacgga atggccccgt ccgccgaccc tttgaagatc ccttcatcta tttaggaaga
      721 agacttgttg acgcggaacc tcaaaaacct aagcgccggc ctccttggtt tcctgatcgt
      781 gcagacgggg gccaccctgg cctggaaggc ctacgcggcc tgtgccaccg ccgggcggga
      841 cggctccccc tcgctttccg ggctcatcaa tacctattac cccccggccg ccgtcaccac
      901 cttgtcgtcg ggcgccacgg tgatcgccct gggcagcgcc tccggttcca ccgtgcccat
      961 ctcggtgggc gacctggtgc tcgtcattca agtgcaggac gcctccatca gcgtgaccca
     1021 aacggccaac tacggggccg ggacggggac cggttcgggc gccaccgcct tcgccagcac
     1081 gggcctctat gaatacgcca ccgccgccag cacggtgccc acgggcgggg gatccctgac
     1141 cctcacggcc ggcctgtcga acacctacca cgaggcggcg gcttccgccg cctcgggcca
     1201 gaagaccttt gaggtcatcc gcgt
//
LOCUS       DASTJJ010000343         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1027427, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000343 DASTJJ010000000
VERSION     DASTJJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027427"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFR02_04275"
     CDS             <1..1056
                     /locus_tag="VFR02_04275"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9869701.1"
                     /translation="LTQPGTTIACGDSHTSTHGAFGTLAFGIGTSQVRDVLATQCLAM
                     DTLKVRRVNVNGTLKPGVTAKDVTLYIIQKLGVKGGIGYAYEYGGDVFDAFTMEERMT
                     VCNMAIEGGARIGYVNPDQTTFDYLKGKPYAPTGEKWDKALAYWRSVASQRDAVYDDV
                     VDFDAADIEPIVTWGITPDQSVGVGAKLPEVGRFSPDEAKTVELAYRHMDLKPGAPIQ
                     GTAINVAFIGSCTNGRISDLRAAAKVAKGRKVAKGVRAFVVPGSQHVKAQAEQEGLDK
                     VFIEAGFEWRSAGCSMCLAMNPDKLKGREISASSSNRNFIGRQGSKDGRTLLMSPAMV
                     AAAAVTGQVTDVRELLK"
     gene            1154..>1331
                     /locus_tag="VFR02_04280"
     CDS             1154..>1331
                     /locus_tag="VFR02_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopropylmalate isomerase"
                     /protein_id="HET9869702.1"
                     /translation="MSKIDQIEGRGVPVEGNDIETDRIIPARYLKEITFTNMGKYPFY
                     DERFDAEGKPKGHPF"
BASE COUNT          270 a          433 c          428 g          200 t
ORIGIN      
        1 ctcacccagc ccggcacgac catcgcctgc ggcgattccc acacttccac ccatggggcc
       61 ttcgggaccc tggccttcgg catcggcacc agccaggtgc gcgacgtgct ggccacccag
      121 tgcttggcca tggacaccct caaggtgcgc cgcgtcaacg tgaacggcac cctgaagccc
      181 ggcgtcaccg ccaaggatgt gaccctctat atcatccaaa aactgggggt caagggcggc
      241 atcggctacg cctacgagta cggcggggac gtcttcgacg ccttcaccat ggaggagcgc
      301 atgacggtgt gcaacatggc catcgagggc ggcgcccgca tcggttatgt gaaccccgac
      361 cagaccacct tcgattacct gaagggcaag ccctacgcgc cgacggggga aaagtgggac
      421 aaggccctgg cctattggag gtcggtggcc tcgcagcggg acgcggtcta tgacgacgtg
      481 gtcgatttcg acgcggccga catcgaaccc atcgtgacct ggggcatcac gcccgaccag
      541 tcggtgggcg tgggggccaa gctgcccgaa gtcgggcgct tttcccccga cgaggccaag
      601 accgtggagc tggcctaccg gcatatggac ctcaaacccg gcgcgcccat ccagggcacg
      661 gccatcaacg tggccttcat cggcagctgc accaacggcc gcatctcgga cctgcgcgcc
      721 gccgcgaagg tcgcgaaggg ccggaaggtg gccaagggcg tgcgggcctt cgtggtgccg
      781 ggatcccaac acgtgaaggc ccaggccgag caggaagggc tggacaaggt cttcatcgag
      841 gcgggcttcg agtggcgcag cgcgggttgt tccatgtgcc tggccatgaa cccggacaag
      901 ctcaaggggc gggagatctc ggcctcctcc tccaaccgca acttcatcgg ccgccagggc
      961 tcgaaggacg gccgcacgct gttgatgagc cccgccatgg tggcggccgc cgccgtcacg
     1021 ggccaagtga ccgacgtgcg ggaactgctg aaataggaca tgggcctaaa agccgcaaaa
     1081 ccgcgaagtt aaatccatcc gcaaaaagaa gtccttggcg ccttggcgtc tttgcggtga
     1141 aagaggtttc gacatgagca agatcgatca gatcgaggga cgcggggtgc ccgtggaggg
     1201 caacgacatc gaaacggacc gcatcatccc ggcccgttac ctgaaggaga tcaccttcac
     1261 caatatgggc aaatacccct tctacgacga gcgcttcgac gcggaaggca agcccaaggg
     1321 ccatcccttc g
//
LOCUS       DASTJJ010000344         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_415538, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000344 DASTJJ010000000
VERSION     DASTJJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415538"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFR02_04285"
     CDS             <1..200
                     /locus_tag="VFR02_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869703.1"
                     /translation="PAEDCTTGEVLRALGRAKASAEVREAAEKCLRACDRVLYAEGAL
                     SPKESLRGWAQALLPRAAKRP"
     gene            249..>1218
                     /locus_tag="VFR02_04290"
     CDS             249..>1218
                     /locus_tag="VFR02_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9869704.1"
                     /translation="MAGSNEELSQEGARLSEQFQKAREEVGKVIVGQDGLLDRLMLGL
                     LARGHLLLEGLPGLAKTLAVKTLSEVLGVSFSRLQFTPDLLPADLLGTRIYNPAKGDF
                     KTQKGPVFANLVLADEINRAPAKVQSALLEAMQEHQVTIGPDTFPLEGPFFVMATQNP
                     IEHEGTYPLPEAQVDRFLMKVLVGYPTPAEEKQIITRMAGGESPQVREVLSASAILKA
                     QKAVDGVLVSETLKDYIVSLVGATRAPKDYRLDSLAPMIEYGASPRASLGLSAAAKAR
                     AFLDGRGYATPEDVKALAGDLLRHRLILTYEAEAQNVKADSLIDQIL"
BASE COUNT          212 a          381 c          435 g          190 t
ORIGIN      
        1 tcccggcgga ggactgcacc accggggagg tgttgcgggc cttgggccgg gcgaaggctt
       61 ccgccgaagt gcgggaagcg gcggagaaat gcctgcgggc ctgcgaccgg gtgttgtacg
      121 cggaaggcgc cctgagcccg aaggagagcc tgcggggctg ggcccaggcc ttgctgccgc
      181 gggcggcgaa gcgtccgtga agcgccgggg gcggtggtcg ggcagggata tcattcaagg
      241 agacggcgat ggcgggatcc aatgaagagt tgagccagga aggcgcgcgg ctgagcgaac
      301 agttccagaa ggcccgggag gaagtgggga aggtcatcgt gggccaggac ggcctcttgg
      361 accggttgat gctgggcctg ctggcccggg gccaccttct tttggaaggg ctgcccggcc
      421 tggccaagac cctggcggtc aagacgctgt cggaagtgct gggggtttcc ttcagccggc
      481 tgcagttcac gccggacctg ctgccggcgg accttttggg cacccgcatc tacaaccccg
      541 ccaaggggga cttcaagacc caaaagggcc ccgtcttcgc caacctggta ttggcggacg
      601 agatcaaccg ggcccccgcc aaggtgcagt cggcgctgtt ggaggccatg caggagcacc
      661 aggtcaccat cggcccggat accttccccc tggaggggcc tttcttcgtg atggccaccc
      721 agaaccccat cgagcacgag gggacctacc ccctgcccga ggcccaggtg gaccggttcc
      781 tgatgaaggt gttggtggga tatcccacgc ccgccgagga aaagcagatc atcacccgca
      841 tggcgggcgg ggaatccccg caggtgcggg aggtcctttc ggcctcggcc atcctcaagg
      901 cgcaaaaggc cgtggacggc gtgttggtca gtgagaccct gaaggactac atcgtttccc
      961 tggtgggcgc cacccgcgcg cccaaggact acaggcttga ttcgttggcg ccgatgatcg
     1021 aatatggggc cagtccccgg gcgtccctgg ggctttcggc ggccgccaag gcccgcgcat
     1081 tcctggacgg ccgcggttac gccaccccgg aggacgtgaa ggccctggcc ggcgacctgt
     1141 tgcgccaccg cctgatcctg acctacgagg cggaagccca gaacgtgaaa gccgattcct
     1201 tgatcgacca aatcctca
//
LOCUS       DASTJJ010000345         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_905055, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000345 DASTJJ010000000
VERSION     DASTJJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905055"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..651
                     /locus_tag="VFR02_04295"
     CDS             22..651
                     /locus_tag="VFR02_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET9869705.1"
                     /translation="MKPHRLILFDLDGTLTRGVSTTRFLFRRAGDEAFFRGLESRWMR
                     DEVGHLALARQVTERMAGWRVRDLERSLERIPKMRGIGHTVRTLKARGFYVALATLGY
                     ELSAAYFQKRYGFDGAWGTTLQVRRGVITGRGMRIFQEHQKAALLKRLARRLRVPLSG
                     AVAVGDSRSDREVFKVAGRRVALNQDGFLKGLADVDLRGDDLRRILGVL"
     gene            648..1544
                     /locus_tag="VFR02_04300"
     CDS             648..1544
                     /locus_tag="VFR02_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9869706.1"
                     /translation="MRLRQNGLLLMLLSDLFFAFVPITVKWVHALGCSAVEVTFFRFT
                     FGVVGALALAAAGFQTLRVVKPGPLFWRGFFGGLAVFFYFISLDYTTAGRATLWNYTY
                     SIWANVFSIFLLKRPAPRHFAWMLALGALGVFLVLGVGPGSAGPGDLAGLLSGASAGA
                     SVVAIKEARRTDNSLSIFMSFSLFGSLFAGAFLAAAAVPGAPPLVQALGGWGTVAGRG
                     LGLLALLGLLGMAAQLFLTQALGHLSLSMGTLLSLLVPVGAALFGLLLLGEPLTPHFL
                     AGTFLVLAACAWLGFSERPDPA"
     gene            1627..>1890
                     /locus_tag="VFR02_04305"
     CDS             1627..>1890
                     /locus_tag="VFR02_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869707.1"
                     /translation="MTGKTGWVEKRQHERVVATIKVSYRVIESKQAQAILAQDSYRST
                     TADQLPGLSQVSPLYEAVTKDVSLGGLSILSEQPMPAGTVLEVG"
BASE COUNT          237 a          629 c          668 g          356 t
ORIGIN      
        1 tgggatcaag aaaggcccgt cgtgaaaccc caccgcctca tcctctttga cctggacggc
       61 accctcaccc ggggcgtttc caccacccgg ttcctcttcc gccgcgcggg ggacgaggcc
      121 tttttccggg gcctggaaag ccggtggatg cgggatgagg tgggccacct ggccctggcc
      181 cgccaggtca cggagcgcat ggcgggctgg cgcgtgcggg acctggaaag gtccctggag
      241 cgcatcccca agatgcgggg catcgggcac accgtgcgga ccctgaaggc ccggggcttt
      301 tacgtggccc tggccaccct gggctacgaa ttgtcggcgg cctatttcca aaagcgctac
      361 ggtttcgacg gggcctgggg caccaccctg caggtgcggc ggggcgtcat cacggggcgg
      421 ggcatgcgca tcttccagga acaccaaaag gccgcgctgc tcaagcgcct cgcccggcga
      481 ctccgggtcc ccctgtcggg cgcggtggcg gtgggcgaca gccgctcgga ccgggaggtc
      541 ttcaaggtgg cggggcgtcg cgtggccctc aaccaggacg ggttcctcaa gggtctggcg
      601 gacgtggacc taaggggcga cgacctgcgc cggatcctgg gggtcctatg aggctgcgcc
      661 agaacgggct gctgctcatg cttttgagcg acctcttctt cgccttcgtg cccatcacgg
      721 tcaagtgggt ccacgccctg gggtgctcgg cggtggaggt caccttcttc cgcttcacct
      781 tcggggtcgt gggggccctg gcgctggcgg cggcgggatt ccaaaccttg agggtggtga
      841 agccgggccc gctcttctgg cggggtttct tcggcggcct ggcggtcttc ttctatttca
      901 tcagcctgga ctacaccacg gccggccgcg ccaccctctg gaactacacc tattcgatct
      961 gggccaacgt cttttccatc ttcctcctca agcgccccgc gccccggcat ttcgcctgga
     1021 tgctggccct gggcgccttg ggggttttcc tcgtgttggg ggtgggcccg gggtcggcgg
     1081 ggccggggga cctggcgggg ctcttgtcgg gcgcctccgc gggcgcgtcg gtggtggcca
     1141 tcaaggaggc ccgccgcacg gacaattcgc tgagcatctt catgagcttc agccttttcg
     1201 ggtcgctctt cgcgggggcc ttcctggcgg ccgcggccgt gcccggagcg ccgcccctgg
     1261 tccaggcctt ggggggctgg ggcaccgtgg cgggccgggg cctgggcctt ttggcccttc
     1321 tggggctgct ggggatggcc gcccagcttt tcctcaccca ggccttgggg cacttgagcc
     1381 tgtccatggg gacgctcttg tcgctcttgg tgcccgtggg cgcggcgctc ttcgggcttt
     1441 tgctcctggg ggagcccctc acgccgcatt ttttggcggg caccttcctg gtgctcgccg
     1501 cctgcgcctg gctggggttc agcgagaggc ccgaccccgc ttaggcccgg gcaattcccc
     1561 cgggcccccc gggccctttt gtggtaaaaa gggtgggaac agatattccc cgggcgaggt
     1621 ggttcgatga cgggaaagac cggttgggtg gaaaaacgcc agcatgagcg ggtggtggcc
     1681 accatcaagg tgagctaccg ggtgatcgaa tccaagcagg cccaggccat cctggcccag
     1741 gattcctacc gcagcaccac ggcggaccag ctgccgggcc tgtcccaggt ctcccccctc
     1801 tacgaggccg tcaccaagga cgtttccctg ggcgggctct ccatcctcag cgaacaaccc
     1861 atgcccgccg ggaccgtgtt ggaggtgggg
//
LOCUS       DASTJJ010000346         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_415705, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000346 DASTJJ010000000
VERSION     DASTJJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415705"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VFR02_04310"
     CDS             <1..919
                     /locus_tag="VFR02_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HET9869708.1"
                     /translation="VPVTATATPTWTPTPLPPTATPTPAGLGSLSVYFMPGVGTDNTN
                     SPHPQIEVVNTGTGPLSLNNVEVRYWFNCDCTGQSVQSWVDWAGLLPGGSSVTGDVRT
                     SVQPTGLGGQTNYVSYQFTGNLVLQPGQRIEIQSRFNKSDWSNMVQDNDWSFTTGSGF
                     TASTHITAYLNGSLVWGQEPASPSPALQAVNLVAFPNVSNGSGVNLSVNLPGTTAGAS
                     AKAVTIGSQPIDPTAVITFRAYTLDGQLVWTKTVAAAELGSSGRRGLFWNEKNMASQA
                     LSSGLYIVTATVTAQGQTSTASTKVAIVK"
     gene            1199..>2315
                     /locus_tag="VFR02_04315"
     CDS             1199..>2315
                     /locus_tag="VFR02_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9869709.1"
                     /translation="MRITKTTTYILFVTALLGGFSQAQAGTATIDSTTTYQYIRGFGA
                     SSAWHESTFSTQLATWFWDSTGMTGNNPNGIGMSMLRCHIPYNNSTSATGGTGISDPG
                     ETAVMKQAIDLGVTQVWVTEWTPPNAYKTNGAPYGSGDPVATTNNTFSGAGTTPANGS
                     DTGYANYLVNYIQYVNAQLNSRGVKVLAVSPQNEPDWNPTYESAIWTAGKFDVFVGSL
                     YSALQAASLNTKIMIPESFADNKSLAATAMDDAAIAPEVAVIGNHLYGLNGATPYSLA
                     GAGFTHLTNQENWETEMSDVSGAANDTSITSGLQIASWVQQCIVGASMNAYHAWWLYP
                     TGNTNEALIGTDNLSTKKLWCLGNWSRFVRPGYYRMGA"
BASE COUNT          465 a          791 c          691 g          368 t
ORIGIN      
        1 ggtccccgtc acggcgaccg caacgcccac ctggaccccc acgcccttgc cccccacggc
       61 cacgcccacg ccggcgggcc tgggcagcct gagcgtttat ttcatgcccg gggtcgggac
      121 cgacaacacc aacagccccc acccgcagat cgaggtggtg aacacgggaa cgggcccctt
      181 gagcctgaac aacgtggagg tgcgctactg gttcaactgc gactgcacgg gccaaagcgt
      241 gcagagctgg gtggattggg cggggctcct gccgggcgga agctcggtca cgggcgacgt
      301 gcgcacctcg gtgcagccca ccggcctggg cggccagacc aactacgtga gctaccagtt
      361 cacggggaac ctggtgctgc agccgggcca gcgcatcgag atccaatcgc ggttcaacaa
      421 gagcgactgg agcaacatgg tccaggacaa cgactggagc ttcacgacgg gctcgggctt
      481 cacggcttcc acccatatca cggcctactt gaacgggtcg ctggtctggg gacaggagcc
      541 cgcgtcgccc tcgccggccc tgcaagcggt caacctggtg gccttcccga acgtttccaa
      601 cgggtcgggg gtcaacctgt cggtcaacct gcccggcacc acggcggggg ccagcgcgaa
      661 ggccgtcacg atcgggtccc agccgatcga cccgaccgcg gtcatcacct tccgggccta
      721 tactctcgac ggccaattgg tctggaccaa gaccgtggcc gccgccgagc tgggaagctc
      781 gggacgccgg ggcttgttct ggaacgaaaa gaacatggcc agccaggccc tgtccagcgg
      841 cctctatatc gtaacggcca ccgtgacggc gcagggacag accagcacgg cgagcaccaa
      901 ggtcgccatc gtgaagtagg gaggggccga ggtccgtccc tgttccccgg gggtttattc
      961 ccccggggag catttttcct tggatgaggg ttcaaaaccg ggtcctgcgg ttaaacgaga
     1021 aagcgctcca ttggaccggt ccaaatttgg ccatgaaata aaaggcatgt tgtttgacgt
     1081 tttaaggcta tagagtgtga agggtgtcgc ccgggcgggt ccgggctgtg ggatactttg
     1141 aaaaccgatg ggccgacgtt tgcggctttt ccaacttaaa tcgtttgaag acgaaaagat
     1201 gcggatcacg aaaaccacta cttatatcct atttgtgacg gccctcctgg gtggattctc
     1261 ccaagcccag gcggggacgg ccaccattga ttccaccacc acctatcaat atatccgtgg
     1321 cttcggggcc tccagcgcct ggcatgagag caccttctcc acccagttgg ccacttggtt
     1381 ctgggattcc accggcatga ccggcaacaa ccccaacggc atcggcatgt ccatgttgcg
     1441 ctgccacatc ccctacaaca actccaccag cgccaccggg ggcacgggca tcagcgaccc
     1501 cggcgaaacg gcggtcatga aacaggccat cgacctgggg gtcacccagg tctgggtgac
     1561 cgaatggacg ccgcccaacg cctataagac caacggcgcc ccctacggca gcggcgaccc
     1621 cgtggccacg accaacaaca ccttcagcgg ggcagggacc accccggcga acggcagcga
     1681 cacgggctac gccaactacc tggtcaacta catccaatac gtcaacgccc agttgaacag
     1741 ccggggcgtc aaggtgctgg ccgtctcgcc ccagaacgag ccggactgga accccaccta
     1801 tgagtcggcc atctggaccg ccggcaagtt cgacgtcttc gtcggatccc tttattccgc
     1861 cctccaagcg gcctccctca acaccaagat catgatcccg gagagcttcg cggacaacaa
     1921 gtcgctggcc gccaccgcca tggacgacgc cgccatcgcc cccgaggtcg ccgtcatcgg
     1981 caaccacctc tacggcctga acggggccac gccctattcg ctggctggcg ccggcttcac
     2041 ccacctgacc aaccaggaga actgggagac cgagatgtcg gacgtcagcg gcgcggccaa
     2101 cgacacgtcc atcacctcgg gcctccagat cgcctcctgg gtgcagcagt gcatcgtggg
     2161 cgccagcatg aacgcctatc acgcctggtg gctctacccc acggggaaca ccaacgaggc
     2221 cctgatcggc acggacaacc tgtccaccaa gaaactctgg tgcctgggca actggagccg
     2281 cttcgtgcgg cccggctatt accgcatggg cgcca
//
LOCUS       DASTJJ010000347         1229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_660526, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000347 DASTJJ010000000
VERSION     DASTJJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660526"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFR02_04320"
     CDS             complement(<1..700)
                     /locus_tag="VFR02_04320"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9869710.1"
                     /translation="MIISVLLFFFTFFFYLRTHCATFNINDSGETIMVCDLLTVGHSP
                     GYPLHALWGRVFCLFPMGQPMFRVTLCSMFTGAVAVVVLYWTLKLVLREHMFSGGKLI
                     DNDPSGVRMSHWMYEWPALFGSLCFAFSYQHWFQVGGCKGGIYALNTFFSLMMLLLVF
                     KMREQGWFIRGFYLMGYFYSLGLAHHWPSSGVLLPCLFWLLLNSQKRVPVPDFLKLAI
                     DPVAIFSVLAVFTAC"
     gene            complement(862..>1229)
                     /gene="tpx"
                     /locus_tag="VFR02_04325"
     CDS             complement(862..>1229)
                     /gene="tpx"
                     /locus_tag="VFR02_04325"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET9869711.1"
                     /translation="TLILSVAPSLDTQVCAIQTRRFNKEIANLPAGVEVLTITADLPF
                     AQGRFCGAEGITLTTLSDHRDMAFGDAYGTHIKELRLEARSIFVIGKDGLVKYVEIVK
                     EVTDQPDYDKALAAAKANA"
BASE COUNT          284 a          346 c          396 g          203 t
ORIGIN      
        1 agcaggccgt gaagaccgcc agcacgctga agatggccac cgggtcgatg gccagcttga
       61 ggaagtcggg cacgggcacc cgtttctggc tgttcaagag cagccagaac aggcagggca
      121 agagcacgcc cgaactgggc cagtggtggg ccaaccccag gctgtagaaa tagcccatca
      181 ggtagaagcc gcggatgaac caaccctgct cgcgcatctt gaagaccaac aacagcatca
      241 tcagggagaa gaaggtgttg agggcgtaga tgccgccctt acagcccccc acctggaacc
      301 aatgctggta ggaaaaggcg aagcagaggc tgccgaaaag ggccggccac tcgtacatcc
      361 aatggctcat gcgcacgccc gaggggtcgt tgtcgatgag cttgcccccg ctgaacatgt
      421 gctcgcgcag caccagcttc agggtccaat aaagcaccac cacggccacc gcccccgtga
      481 acatcgaaca gagcgtgacg cggaacatgg gctggcccat gggaaaaaga cagaagaccc
      541 ggccccagag ggcgtgcagg gggtagccgg gggaatggcc cacggtcagg aggtcgcaga
      601 ccatgatggt ctcgcccgaa tcgttgatgt tgaaggtggc gcaatgggtg cgcaggtaga
      661 agaagaaggt gaagaaaaag aggaggaccg aaatgatcat ggggcgcgcg tgccgccttg
      721 aaggattttc aaaaaaacca cactaattta ccgccaagac gccaagttca ccaaggtaag
      781 ttcgggaaaa gaatccccaa aaaccccgct tcccttggtg tcttggtgcc ttcgtggtga
      841 aaatcagcct tttaccgagt tttaggcgtt cgctttcgcc gccgccaaag ccttatcgta
      901 atcgggctgg tcggtgactt ccttcacgat ctcgacatac ttgaccaggc cgtccttgcc
      961 gatcacgaag atcgaccggg cttccaggcg cagttccttg atgtgggtgc cgtaggcgtc
     1021 cccgaaggcc atgtcccggt ggtcggacag ggtggtcagg gtgatgccct cggcgccgca
     1081 aaagcggccc tgggcgaagg gcaggtcggc cgtgatggtc agcacttcga ccccggccgg
     1141 caggttggcg atctccttgt tgaaacggcg ggtctggatg gcgcagacct gggtgtccag
     1201 cgagggcgcc acggacagga tgagggtct
//
LOCUS       DASTJJ010000348         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1394559, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000348 DASTJJ010000000
VERSION     DASTJJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394559"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1648
                     /locus_tag="VFR02_04330"
     CDS             <1..>1648
                     /locus_tag="VFR02_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9869712.1"
                     /translation="QMSFSGSSTSWQDPSTWGSLTFAGQTVYVNNSPKMAMPGAVVAE
                     MDPLGGKKNASTEGDLIWGFNGGDLGGFTGKVSAETVTLTEGNGAMRVDTDGSQGWNQ
                     GLAVCAAVPLADQWEKFKAITMDIYFPAGSLAKAGYGEVYLVTQAPANNWNQIKMSVH
                     EGWNHIRQDVDGSQFKGGLTKVFLVFNSGGPIAGSVVIDNIRGIEKGAAAKLTGKVVD
                     ASGRPVAGAIVAIAKQELTPGADGSFQADLPADDYTAEVFAPGFKSDKADVKVAAGQP
                     NQWAVTLQADSYKVKPAVADIFFDKKIRTINPHYLFGMNIAAWYDPKWMTDPTGLERT
                     EAISSYFRVPGGAYGNIFNWRTGAVYANDGTTVQWTPDFNWPKMAEFVQSVPNSEILL
                     IANIMTGTPQDALDWIADIKARGIKLKYVELGNEPDYAADLAYQGQTQYWTVIDNYCK
                     HYLEFAKAIKGKYPDLKLMGPCPAQVENRERKEGEPWLAAADSPWWVEKFLEECGPYV
                     DVVSVHSYPYWSNDSDGNLLSKVEWWKQWIPKIRAAIQKNI"
BASE COUNT          335 a          536 c          533 g          244 t
ORIGIN      
        1 cagatgtcct tctcgggcag ttccaccagt tggcaggacc cctccacctg gggaagcctg
       61 accttcgccg gacagacggt ctacgtcaac aactccccca agatggccat gccgggggcc
      121 gtggtggccg aaatggaccc cctggggggc aagaagaacg cctccacgga gggcgacctg
      181 atctggggct tcaacggcgg cgacctgggc ggcttcacgg gcaaggtgag cgccgagacc
      241 gtgaccctga ccgagggcaa cggggccatg cgggtggaca ccgacggttc gcagggttgg
      301 aaccaggggc tggcggtctg cgccgccgtg ccgctggccg accaatggga aaagttcaag
      361 gccatcacca tggacatcta tttccccgcg ggcagcctgg ccaaggccgg ttacggcgag
      421 gtgtacctgg tgacccaggc cccggccaac aattggaacc agatcaagat gagcgtgcat
      481 gagggatgga accacatccg ccaggacgtg gacggcagcc agttcaaggg cgggctcacc
      541 aaggtcttcc tggtcttcaa ttcgggcggc cccatcgcgg gctccgtggt catcgacaac
      601 atccggggca tcgagaaggg cgcggcggcc aagctgaccg gcaaggtggt ggacgcctcc
      661 ggccgtccgg tggcgggcgc catcgtggcc atcgccaaac aggaactgac gcccggggcg
      721 gacggctcct tccaggccga ccttccggcc gacgactata ccgccgaggt cttcgcgccc
      781 ggtttcaagt ccgacaaggc ggacgtgaag gtggcggcgg gacaacccaa ccagtgggcc
      841 gtgaccttgc aggccgattc ctacaaggtg aagccggccg tggcggacat cttcttcgac
      901 aagaagatcc gcaccatcaa cccccattac ctgttcggca tgaacatcgc ggcctggtac
      961 gaccccaagt ggatgaccga tcccaccggg ttggaaagga ccgaggccat cagctcctac
     1021 ttccgcgtcc cgggcggcgc ctacggcaac atcttcaact ggcgcacggg cgcggtctac
     1081 gccaatgacg gcaccaccgt gcagtggacc ccggacttca actggcccaa gatggccgag
     1141 ttcgtgcagt ccgtccccaa ttcggagatc ctgctcatcg ccaacatcat gacgggcacg
     1201 ccccaggacg ccttggactg gatcgccgac atcaaggccc ggggcatcaa gctcaagtac
     1261 gtggagctgg gcaacgagcc cgactacgcg gccgacctgg cctaccaggg ccagacccag
     1321 tactggaccg tcatcgacaa ctactgcaag cactacctgg agttcgccaa ggccatcaag
     1381 ggcaagtatc cggacctgaa gctgatgggc ccatgcccgg cccaggtgga gaaccgcgag
     1441 cgcaaggaag gcgagccctg gctggccgcc gccgattcgc cctggtgggt ggagaagttc
     1501 ctggaggagt gcggccccta cgtggacgtg gtgtccgtgc acagctatcc ctactggagc
     1561 aacgacagcg acggcaacct gctgtccaag gtggagtggt ggaagcagtg gatccccaag
     1621 atccgcgcgg ccatccaaaa gaacatcc
//
LOCUS       DASTJJ010000349         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_660784, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000349 DASTJJ010000000
VERSION     DASTJJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660784"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..721
                     /locus_tag="VFR02_04335"
     CDS             101..721
                     /locus_tag="VFR02_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869713.1"
                     /translation="MAEETRAPKKKSGEPRSLWRLLFVEALAAAGFYWMTRHAVPPGW
                     KGGTFRLNALFFLAAYPLLFIYRWVLPGGFFGVLLGPAALVLGLALGVSTYLWMEYGF
                     LWAGLPYAGILRAHWAVLGALFVPLYLMFFRNLMGISRLYYTMEECLALCLWVGLGGG
                     GGFLLGKLLDDRLQDAPFFQHHRFLIWLFLIWLGTVAALLLARKRK"
     gene            724..1203
                     /locus_tag="VFR02_04340"
     CDS             724..1203
                     /locus_tag="VFR02_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HET9869714.1"
                     /translation="MARVFPGRYTAQIEGPFVVFLIGMRVNHFWKVWKWLPVALSMPP
                     LLKALREDPAKGWLHGEAFIGNRGPLLVQYWRSFEDLERFARSPEEPHLEAWQAFNRR
                     VGVRGDVGVWHETYRVEAGAYECIYNNMPVFGLAQAARHLPADAAYETARRRLARKE"
     gene            1266..1460
                     /locus_tag="VFR02_04345"
     CDS             1266..1460
                     /locus_tag="VFR02_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869715.1"
                     /translation="MLNPEQVQLASTAVRGPRAALSNFFLYSAENAMVRQALDRGRRN
                     SRGNSAGASPDGPRDPGSQP"
     gene            1457..>2160
                     /locus_tag="VFR02_04350"
     CDS             1457..>2160
                     /locus_tag="VFR02_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869716.1"
                     /translation="MRGRRQPGRPRAERPGVAELRGSLVLYAAAAVLAFAPGLFTGRS
                     LFEGDLINLNVPYWDFLRGCLSRGQWPLWCPFLFAGQPFLADPNTMMWYPPAYLFLLP
                     SWVWGMGLFGAFHLFMVLVGAHLWLRSAGFSPFARRVGALTFGLSGFFWLEAVHPQVV
                     AGYAWLPWWGLALDRLAQTGERRWAFAAGASFALMFLSGHFQVPLGAIYGGGAYFCFR
                     SWAAARAGGGVPVDWK"
BASE COUNT          262 a          702 c          736 g          460 t
ORIGIN      
        1 tcccctttcg ccaagcccaa gggccgctga attcccgagc gcccgcgtcc cggctgggat
       61 accatggggc ggtcgtcgat cccacaggcg aggtccccct ttggccgaag aaaccagagc
      121 cccgaagaaa aagtccggcg aaccccggtc cctttggagg ctgcttttcg tggaagcctt
      181 ggcggcggcc ggtttctact ggatgacccg ccacgccgtg cctcccggct ggaagggagg
      241 gaccttccgg ctcaacgccc tctttttcct ggcggcctat cccttgctct tcatttaccg
      301 ctgggtcctg cccggcgggt ttttcggcgt gctcctgggt ccggcggccc tggtcctggg
      361 gttggccttg ggcgtttcca cctacctttg gatggaatac gggttccttt gggcggggct
      421 cccctatgcc ggcatcctgc gcgcccactg ggcggtcttg ggggcgctct tcgtgccgct
      481 ttacctgatg ttcttccgga acctgatggg catcagccgc ctctactaca ccatggagga
      541 atgcctggcc ctttgcctct gggtggggct gggcggcggg ggcgggttcc tgctggggaa
      601 gcttttggac gaccgtctgc aggacgcgcc cttcttccag caccaccgct tcctgatctg
      661 gcttttcctg atctggttgg gcacggtggc ggccctgctc ctcgcccgga aacggaagtg
      721 accatggccc gcgtcttccc cggccgctat acggcccaga tcgagggacc tttcgtggtc
      781 ttcctcatcg gtatgcgggt caatcatttt tggaaggtct ggaagtggct gcccgtggcc
      841 ctgtccatgc cccctttatt gaaggcgttg cgggaggatc cggccaaggg ctggctgcat
      901 ggggaggcct tcatcgggaa ccggggcccc ttgttggtgc agtattggcg ttcgttcgag
      961 gacctggaac ggttcgcccg gtcgcccgag gaaccccacc tggaggcctg gcaggccttc
     1021 aaccgccggg tgggggtgcg gggggacgta ggcgtctggc acgagaccta ccgggtggag
     1081 gcgggggctt acgaatgcat ctacaacaac atgccggtct tcggcctggc ccaagccgcc
     1141 cggcaccttc cggcggacgc ggcctatgaa acggcgcgcc ggcgcctggc ccgcaaggaa
     1201 tagacccggg gggaaggcgt cattgtatga tgaggccgtc gtgaaccgcg ccggcgaggg
     1261 aacccatgct gaaccctgaa caagtccaac tggcctcgac cgccgtgcgc ggaccgcgtg
     1321 cggccttgtc caacttcttc ctttattccg ccgagaacgc catggtccgg caggccctgg
     1381 atcgggggcg tcggaattcg cggggaaatt cggccggcgc ctccccggac gggccccggg
     1441 atccggggtc ccagccatga ggggacgccg tcagccgggc cgtccccggg ccgaacgccc
     1501 gggcgttgcg gagctccggg ggagcctggt cctttacgcc gccgccgcgg tcctggcctt
     1561 cgctcccggg ctcttcacgg gccggtccct gttcgagggg gacctgatca acctcaacgt
     1621 cccctattgg gattttctgc gcggttgtct ttcgcgcggg caatggcccc tttggtgccc
     1681 cttccttttc gcgggccaac ccttcctggc ggaccccaac accatgatgt ggtatccccc
     1741 cgcctatctt ttcttgctgc cgtcctgggt ttggggtatg gggcttttcg gggccttcca
     1801 tctctttatg gtcctggtgg gcgcccatct ttggctgaga agcgccggct tttcgccctt
     1861 cgcgcgccgg gtgggggcgc tgaccttcgg gctctcgggt tttttctggc tggaggccgt
     1921 ccatccccag gtggtggcgg gctacgcttg gctgccctgg tggggcctgg cgttggaccg
     1981 cttggcccag accggggaaa ggcgttgggc cttcgcggcc ggggcgtcct tcgccttgat
     2041 gttcttgtcc gggcatttcc aggtccccct gggggcgatc tacgggggcg gcgcctattt
     2101 ctgcttccgg tcgtgggccg cagcccgggc cggaggggga gtgcccgtgg attggaagag
//
LOCUS       DASTJJ010000350         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1517251, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000350 DASTJJ010000000
VERSION     DASTJJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517251"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFR02_04355"
     CDS             <1..707
                     /locus_tag="VFR02_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9869717.1"
                     /translation="MVSQVKRRVVTYGLTAGVQVQARKVKYEKAGSVFEVALEGERLG
                     AIRLGVPGRFNIQNALAAVSVALELKVPFKAIAAGLAKFHGAQRRFEVKGTYKGAVVV
                     DDYAHHPTAVAETLTAAKRFAGNKKLVVVFQPHLYSRTQLLRDKFGKAFADADEVLVT
                     AVYPSREKPIPGVTGKTLVEAIRENGHPSVQSFEDRTALMEYLKAHLTDKHVLLTMGA
                     GDVWKLGQDLVQKHKG"
     gene            856..1803
                     /gene="murB"
                     /locus_tag="VFR02_04360"
     CDS             856..1803
                     /gene="murB"
                     /locus_tag="VFR02_04360"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET9869718.1"
                     /translation="MDLAVLKGLVQGRVELGYPMAKATSYHLGGPAGALVEPRDLEDL
                     KALMGWLAGTKTPYLVLGGGSNLLFADEGFGGVVIRLGRGFQGVAVEGNLVRVGAATQ
                     LVTVLKRTREAGLGGVEFFAGIPGTLGGAVAGNAGAKKAWIGTTVESLTLVAPGGAVK
                     ELGKSDYTYGYRQSSLKMSGNVLVEAVLKLKKEPKADIERKVREYFKVRRGKQPRVEP
                     NAGSVFKNPEGNFAGRLTESLGLKGFKVGGAAVSRVHANFIVNDGTATAHDVVAVMRE
                     VQKRVLAEYGIRLEPEILPLGDWDWGGIKDVWWNMERKF"
     gene            1888..2850
                     /locus_tag="VFR02_04365"
     CDS             1888..2850
                     /locus_tag="VFR02_04365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9869719.1"
                     /translation="MEPKAGSKKLRVGVLMGGWSTERAVSLVSGGMVAEGLRAAGFTP
                     VPLDLRAADRRERKLAARLKAARLDAAFIALHGGWGENGGAQELLGRLRIPYTGSGPL
                     ACGLAMQKACAKLVFEANRIPTAPWQALERKADNGRRGREVSLAPPLVVKPADGGSAI
                     GVSILRDRRGLDAALALAFKTSRWVLVEKFIAGMEVTAGVLEGKALPLIEIVPKNDFY
                     DYDAKYTPGRSTHILPARLSARTAKKVRALALAAGKALGCRDFYRVDVIVPKKGDPQV
                     LEVNTVPGMTATSLFPEAAAKAGTPFPKLLKRLVALALGRKGRD"
     gene            2850..3878
                     /locus_tag="VFR02_04370"
     CDS             2850..3878
                     /locus_tag="VFR02_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9869720.1"
                     /translation="MYYRGSRVSLSESQPRFYKKPEPRTWRERAREKPREKMFRLSLR
                     LVREAKERKPEREERVVHGFYRPNRLTTAPVRETARTGGAAEEKPFRLPAGMGRAAGV
                     LLKAGLVVGLVGALAWSKVRTSDLLQQMSGLTLARISVVGTHFLTEDQVAQAVRAPLG
                     ENMFKLDLAAVSAQVGSLSWVKRVFVERRLPKTLLISVVERRPAALLDEGSLYAVDGE
                     GRILAPSPALSGVDLPVVSGLSFPPEAVGTTEEAGSLKPALDFLAFLRKQDGSLAQDV
                     SEVNLSQPGCLQVTFLDGVTARFNPGVTDEELRHMALVLGDLNEKGKKAASLDFRYKG
                     EVLVRARQ"
     gene            3972..>4445
                     /gene="ftsA"
                     /locus_tag="VFR02_04375"
     CDS             3972..>4445
                     /gene="ftsA"
                     /locus_tag="VFR02_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9869721.1"
                     /translation="MAKEIKQDVVVGLDLGTTKVCTIIGELDDEGQVHIIGVGTTPST
                     GLKKGSVVNIEQTIQSIKKSVQDAERMAGVEVRSVFAGVAGGHIKGLNSRGVIAVSRR
                     DKEITEDDRERVIEAAQAIAIPLDREIIHVIPQEYIVDDQDGIKNPVGMSGVRLEA"
BASE COUNT          803 a         1252 c         1708 g          682 t
ORIGIN      
        1 ccatggtgtc ccaggtcaag cgccgggtgg tgacctacgg gctcacggcg ggcgtccagg
       61 tgcaggcccg caaggtgaaa tatgagaagg ccggatccgt ctttgaggtg gcgctggagg
      121 gggaacgcct gggggccatc cgcctggggg tgccgggccg cttcaacatc cagaacgccc
      181 tggcggcggt gtcggtggcg ctggagctca aggtgccctt caaggccatc gcggcggggc
      241 tcgccaagtt ccacggcgcc cagcgccggt tcgaggtgaa gggcacctat aagggcgccg
      301 tggtggtcga cgactacgcc caccatccca cggcggtggc cgagacgctc acggccgcca
      361 agcgtttcgc ggggaacaag aagctggtgg tggtcttcca gccccacctc tattcccgca
      421 cccaactgct gcgcgacaag ttcggcaagg cctttgccga cgccgacgag gtgctggtga
      481 cggccgtgta tccttcccgg gaaaaaccca tcccgggcgt gacgggcaag accctggtgg
      541 aggccatccg ggagaacggg cacccgtcgg tgcagtcctt cgaggaccgg acggccctga
      601 tggaatacct gaaggcgcac ctgaccgaca agcacgtgct cctgaccatg ggcgcggggg
      661 acgtgtggaa gctgggccaa gatctagtgc aaaagcacaa gggctgaaag atatgaccac
      721 acacacccaa gggtggcccc attccacgga cttgatgtgg gggcaggcac caaggcacca
      781 agggaggcta aagaaaaaaa ttgttccttc cgttcttggt gtcttggtgc cttggtggtg
      841 atggaggttt ccgcggtgga tcttgcggtc ctgaagggcc tggtccaagg ccgggtggaa
      901 ttgggatatc ccatggccaa ggccaccagc taccacctgg gaggcccggc gggggccctg
      961 gtggaaccca gggacctgga ggacctgaag gccctgatgg gttggttggc ggggacgaag
     1021 acgccctacc tggtgttggg gggcggcagc aacctgctct tcgccgatga ggggttcggc
     1081 ggcgtggtga tccgcctggg acgggggttc cagggcgtgg cggtggaagg gaacttggtc
     1141 cgcgtggggg ccgccaccca attggtgacg gtgctcaagc gcacccggga agcgggtctg
     1201 ggaggggtgg agttcttcgc cggcatcccg ggcaccctgg gcggggccgt ggcgggcaac
     1261 gcgggggcca agaaggcctg gatcgggacg acggtcgagt ccctgaccct ggtggcgccg
     1321 ggcggggccg tgaaggagct ggggaagtcg gactatacct acgggtaccg ccagtcgtcg
     1381 ttgaagatga gcgggaacgt gctggtggag gcggtcttga agctgaagaa ggagcccaag
     1441 gccgacatcg agcggaaggt gagggagtac ttcaaggtgc ggcggggcaa gcagccccgg
     1501 gtggagccca acgcggggag cgtgttcaag aaccccgagg ggaacttcgc cggaaggctc
     1561 accgagtcgc tggggctgaa gggcttcaag gtggggggcg cggcggtgag ccgggtgcac
     1621 gccaacttca tcgtgaacga cggcacggcc accgcccacg acgtggtggc ggtgatgcgg
     1681 gaagtgcaga aaagggtcct ggccgaatac gggatccggt tggaacccga gatcctgccc
     1741 ctgggggact gggactgggg ggggatcaag gatgtttggt ggaacatgga acggaagttt
     1801 tgagttttta gtcctgagtg ttgagtgttc gagcttgaaa ccaagagctc aaaaccgggt
     1861 ggggaacgga taggtacaag gaaagcaatg gaaccgaaag ccgggtcgaa aaaactgagg
     1921 gtgggcgtgc tcatgggcgg gtggagcacc gaacgggccg tatccctggt gtcgggcggg
     1981 atggtggccg agggcctgcg cgcggccggg ttcacgcccg tcccgctgga cctgcgcgcg
     2041 gcggaccgcc gggagcggaa actggcggcg cggctgaagg ccgcccgcct ggacgcggcc
     2101 ttcatcgccc tgcacggcgg ctggggcgag aacggcggcg cccaagagct gctgggccgc
     2161 ctgcggatcc cctatacggg gtccgggccc ctggcctgcg gcctggccat gcagaaggcc
     2221 tgcgccaagc tggtcttcga ggccaaccgg atccccacgg cgccctggca ggcgctggaa
     2281 cgcaaggccg acaacggccg gcgggggcgc gaagtgtccc tggcgccgcc cttggtggtg
     2341 aagccggcgg acgggggatc ggccatcggc gtgagcatcc tgcgggaccg ccggggattg
     2401 gacgccgccc tggccctggc cttcaagacc agccgctggg tgctggtgga aaagttcatc
     2461 gccggcatgg aagtgaccgc cggggtgctg gagggcaagg ccctgccgct catcgagatc
     2521 gtgcccaaga acgactttta cgactatgac gccaaataca cgccgggccg gtcgacccac
     2581 atccttccgg cccggctttc ggcgcggacg gcgaagaagg tgcgggcctt ggccctggcg
     2641 gcggggaagg ccttgggttg ccgggacttt taccgggtgg atgtgatcgt gccgaaaaag
     2701 ggggacccgc aggtcctgga agtgaacacc gtgcccggca tgaccgccac cagcctgttc
     2761 ccggaggcgg cggccaaggc ggggacgcct ttcccgaagc tcttgaagcg cctggtggcg
     2821 ctggccctgg gccggaaagg ccgggactga tgtattaccg ggggtcgcgg gtctccctga
     2881 gcgaaagcca gccccgtttt tataagaagc ccgagccccg cacctggcgg gaaagggccc
     2941 gggagaagcc gcgggaaaag atgttccgcc tgtccttgcg gctggtgcgg gaggcgaagg
     3001 aaaggaagcc ggaacgggaa gagcgggtgg tccacggttt ttaccgcccc aaccgcctga
     3061 ccacggcgcc ggtccgcgaa acggcccgga cggggggggc ggcggaggaa aaacccttcc
     3121 gcctgccggc ggggatgggc cgggcggcgg gagtcctctt gaaggcgggg ttggtggtcg
     3181 ggctggtggg cgccttggcc tggtcgaagg tgaggacctc ggacctgctg caacaaatgt
     3241 cgggcctgac gctggcccgg atcagcgtgg tcgggacgca tttcctgacg gaagaccagg
     3301 tggcgcaggc cgtgcgcgcg cccctggggg agaacatgtt caaactggac ctggcggccg
     3361 tctcggccca ggtggggagc ctgagctggg tgaagcgtgt gttcgtggag cggcggttgc
     3421 ccaagacgct cctgatctcg gtggtggagc gccggcccgc ggccctgttg gacgagggca
     3481 gcctctacgc ggtggatggg gaaggccgca tcctggcgcc ctccccggcg ctgtcggggg
     3541 tggacctgcc ggtggtgtcg gggctgtcct tcccgccgga ggcggtgggg acgaccgagg
     3601 aggccggaag cttgaagccg gccctggatt ttttggcctt cctgcgcaag caggacgggt
     3661 ccttggccca ggacgtgtcg gaagtgaacc tgtcccagcc cgggtgcctg caggtcacct
     3721 tcctggacgg ggtgacggcc cggttcaacc cgggggtgac ggacgaggaa ttgcgccaca
     3781 tggcgctggt gctgggggac ttgaacgaga aggggaaaaa ggcggccagc ttggacttcc
     3841 gctataaggg cgaggtcctg gtcagggcga ggcagtgaaa gttattaatt atgagttttg
     3901 agttttaagt tgtggaacta agaactcaaa actaaaaact caaaactgaa gtcgaggaag
     3961 gggtggacga gatggcaaaa gagatcaagc aggatgtggt ggtggggctg gacctgggga
     4021 cgaccaaggt ctgcaccatc atcggcgaac tggacgacga ggggcaggtc cacatcatcg
     4081 gggtgggcac cacgccctcc acggggctca agaagggctc ggtggtgaac atcgaacaga
     4141 ccatccagtc gatcaagaag tccgtgcagg acgccgagcg gatggccggg gtggaggtgc
     4201 ggtcggtctt cgcgggcgtg gccgggggcc acatcaaggg gctcaacagc cggggcgtca
     4261 tcgcggtgtc gcgccgggac aaggagatca ccgaggacga ccgggagcgg gtcatcgagg
     4321 ccgcccaggc catcgccatc cccctggacc gggagatcat ccacgtcatc ccgcaggaat
     4381 acatcgtgga cgaccaggac ggcatcaaga accccgtggg catgtcgggg gtgcgcctgg
     4441 aggcc
//
LOCUS       DASTJJ010000351         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_781952, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000351 DASTJJ010000000
VERSION     DASTJJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781952"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..222)
                     /locus_tag="VFR02_04380"
     CDS             complement(43..222)
                     /locus_tag="VFR02_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869722.1"
                     /translation="MTKKMLVLILGCLLPAAGAWAKDDALARAASVPLGERAAFYLQS
                     YLDGLNILEGAKGKA"
     gene            417..749
                     /locus_tag="VFR02_04385"
     CDS             417..749
                     /locus_tag="VFR02_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869723.1"
                     /translation="MPFNQLESYRELDRELARLKTHFEYFQNAEPGDLVDWAVYFGKW
                     AERAQDLELEWAEPYARLAGMVVKGLTGSRPPREAFDLLIRGMRGAQMDIQRQVERAL
                     RQRSLVGV"
     gene            complement(746..>1009)
                     /locus_tag="VFR02_04390"
     CDS             complement(746..>1009)
                     /locus_tag="VFR02_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HET9869724.1"
                     /translation="APNNGGWQWTASTGCDPQPYFRVFNPRLQSERFDPEGFYIREHV
                     PELAGLDAKAIHAPEGLLRPAGYPAPIVDHAERKERALALYKV"
BASE COUNT          165 a          325 c          344 g          175 t
ORIGIN      
        1 gcagcgcccg cccggattgt agaaaaggga agcccgtagg ggttaggcct tgcccttggc
       61 cccctccaaa atgttcagcc catccaggta gctttgcagg tagaaagccg cccgctcccc
      121 caagggcacc gaagcggcgc gggcgagggc gtcgtccttg gcccaggccc ccgcggcggg
      181 gagcaggcat cccaggatca agaccagcat ttttttcgtc atggcgccct cccggcggtg
      241 gatgtcccga gattctaatc cccccgggcg ccgggccgtg tcttttttac ccggcactgg
      301 gtaaaagggt ccggccgcgc gcctttcgcc gtccccaaac cctttgaaaa ccccgccgcc
      361 gcccggcatg gtccttgctt ttcccatggg tgctgaaaga ccacaaggag gcgcgcatgc
      421 cgttcaacca attggaatcc taccgggaac tggaccggga gctggcgcgg ttgaagaccc
      481 atttcgagta tttccagaac gccgaacccg gggacttggt ggattgggcg gtctatttcg
      541 ggaaatgggc cgaacgggcc caggacctgg agctcgaatg ggccgagccc tatgcccgcc
      601 tggccggcat ggtggtgaag ggcctgaccg ggtcccgccc gccccgggag gccttcgacc
      661 tcttgatccg gggcatgcgg ggcgcccaga tggacatcca acggcaggtc gagcgggccc
      721 tgcgccaacg gagcctggtg ggcgtctaaa ccttgtagag cgccagggcc ctttccttgc
      781 gttcggcgtg gtccacgatg ggggccgggt agcccgccgg gcgcagcagc ccctcggggg
      841 cgtggatggc cttggcgtcc agacccgcca attcgggcac atgctctctt atatagaagc
      901 cctcggggtc gaaccgctcg ctctgcagcc gggggttgaa gacgcggaaa taaggctggg
      961 ggtcgcagcc ggtggaggcg gtccattgcc agccgccgtt gttgggggc
//
LOCUS       DASTJJ010000352         1483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_782024, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000352 DASTJJ010000000
VERSION     DASTJJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782024"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VFR02_04395"
     CDS             complement(<1..1350)
                     /locus_tag="VFR02_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869725.1"
                     /translation="MPFLLLLVLGFCALAAGCTGSGAVNPLTPQQATVADTASASSSD
                     VIYWTSFPVTFANSDSLNGVDLVSSKSGWACGNNGLILRYDGQTWSKVKLGLAQSENF
                     MAVGFADDGNGWIVGTHGAILGYKGGSWSLAPSPTQQNLYGLTVLPDRTAWAVGANGT
                     LLRYNGVSWSVVDLGKLDPPVTDDLNDVNLSDSNNGWAVGNNGVILRYDGQSWKPFAA
                     PPTTERLNSVSTLNNLQAWIVGAYGTILRFNGTTWSSLGTAFSGFDLYQVFMRGESDG
                     WAVGQNGTILYYDGSRWISHPKPDSKPSLNALSFSGDTGFAMGQAGAIYQFHAKGESP
                     QFSFLFKGELSKPTAPDQAVTLTYTVLNQTLKTSPEVSLELDLPKGFKPLVEGTPTPG
                     PSVSPTPAASPTSTPQPSLTPGVPLPGVAGRTLASTASIALTSGWKLDGDKVRWDLGP
                     "
BASE COUNT          250 a          448 c          551 g          234 t
ORIGIN      
        1 cgggcccagg tcccaccgga ccttgtcccc atccagtttc caacccgagg tcagggcgat
       61 ggaggcggtg gaagccaggg tcctcccggc caccccgggc agggggaccc cgggggtcaa
      121 ggacggttgg ggcgtggagg tcggggaagc ggccggggtg ggcgagacgg aaggcccggg
      181 cgtgggggtc ccctccacca agggcttgaa gcccttgggc aggtccagct ccaaggacac
      241 ttcgggcgag gtcttgagcg tctggttgag gaccgtgtag gtgagcgtga ccgcttggtc
      301 gggggccgtc ggcttggaca gttccccctt gaacaggaag gagaactggg gcgactcccc
      361 cttggcgtgg aactggtaga tggccccggc ctggcccatg gcgaagcccg tgtcccccga
      421 gaaggacaag gcgttcaggg aaggtttgct gtcgggcttg ggatgggaga tccagcggga
      481 cccgtcgtaa taaaggatgg tgccgttctg ccccacggcc caaccgtcgc tctcgccccg
      541 catgaagacc tggtagaggt cgaaacccga gaaggccgtg cccaggctgg accaggtggt
      601 gccgttgaag cgcaggatgg tgccgtaggc ccccacgatc caggcctgca ggttgttgag
      661 ggtcgagacc gaattgagcc gttcggtggt gggcggggcc gcgaagggtt tccaggattg
      721 gccgtcgtag cgcaggatga cgccgttgtt gcccaccgcc caaccgttgt tggagtccga
      781 caggttgacg tcgttcaggt cgtccgtcac gggcgggtcc agcttgccca ggtccaccac
      841 gctccaggaa accccgttgt agcgcagcag ggtgccgttg gcgcccacgg cccaggccgt
      901 gcggtcgggc agcacggtga ggccgtagag gttctgctgg gtgggcgagg gcgccaggct
      961 ccaggaaccg cccttatagc ccaggatggc gccgtgggtg cccacgatcc agccgttgcc
     1021 gtcgtccgcg aaacccacgg ccatgaagtt ctcgctctgg gccaatccca gcttcacctt
     1081 ggaccaggtc tgcccgtcat aacgcaggat caggccgttg ttgccgcagg cccagcccga
     1141 cttggaggag accaggtcca ccccgttgag gctgtccgaa ttggcgaagg tcaccgggaa
     1201 ggaggtccaa tagatcacgt ccgacgagga agcgctggcg gtgtccgcca cggtggcctg
     1261 ctggggggtg agggggttga ccgccccgct gcccgtgcag ccggcggcga gggcgcaaaa
     1321 gcccaggacc aagaggagta aaaaaggcat ggacctatcc taccggggtt tggaaaggcg
     1381 cgcaaggctc ggggccgggg cgtccccctt gggacgctga agcgggcgtc cgggttcccc
     1441 gggcacaaaa aaagccgccc tccggttccg gagggcggct tgg
//
LOCUS       DASTJJ010000353         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_49617, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000353 DASTJJ010000000
VERSION     DASTJJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49617"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFR02_04400"
     CDS             <1..376
                     /locus_tag="VFR02_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HET9869726.1"
                     /translation="EAMLEQIQASDQWNYYQAKGIKAAVLGSKIEILKGERRAPSKED
                     LEKMEQYKADQEKISGQAKEYERSSEERLGHHESLAFSVTFFQVAIAVGAISALARRR
                     AFWLVSLGFGAVGLVFLVLGLF"
     gene            427..831
                     /locus_tag="VFR02_04405"
     CDS             427..831
                     /locus_tag="VFR02_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9869727.1"
                     /translation="MESLSKLNAEAYGALRSVAGFLFLFHGAQKLFGLFLDPSLMPHF
                     GTQMWFGGVIELVGGVLIMVGLCTRWAAFLASGEMAVAYFQFHWKFVFTSAFFPLVNH
                     GEMAVLYCFVFLFIATQGPGKWSLDGMLKKKK"
     gene            complement(895..1173)
                     /locus_tag="VFR02_04410"
     CDS             complement(895..1173)
                     /locus_tag="VFR02_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9869728.1"
                     /translation="MTKSEMLSALAEKTGFKKKDVQTLMLGLNDLIYKTLKKEKQIKL
                     DGLGVFQLKDRKARMGRNPATGETIKIAAKRVVKFRVIKALKDEVLGK"
     gene            complement(1290..>2918)
                     /locus_tag="VFR02_04415"
     CDS             complement(1290..>2918)
                     /locus_tag="VFR02_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HET9869729.1"
                     /translation="GPVLAAQFGFCPGFLWLLAGVCLAGAVQDFMVLAASVRRKGKSL
                     AEIARHEISPLAGLVGSLAILIIVIVALAGLGITVVNALKDSPWGVFSIGMTIPIALL
                     TGLWMSRLRPGRIAEASAVGVTLVFLAVIFGNLVPGSPLDPYLRLSREGVIVALALYA
                     FVASVLPIWALLTPRDYLSTYMKLGTIGALVAGILWVHPDLQMPAFTSYVHGGGPIIH
                     GKLFPFMFITIACGAISGFHALVASGTTPKMLDKEGDARVIGYGAMLMEGLVGIVALL
                     ASCSLFPADYFAINVPADKFAALGMTPVNLQALDQAVGEDVAGRPGGAVSLAVGFAQI
                     FSGLPGLKGLMAYFYHFMIMFEALFILTTIDAGTRIGRFLVQEYGGRLWPRFADNKWL
                     PGTLLSTALVVGAWTYLLSTGTVGTIWPMFGAANQLLASVALAVATSAIINTGRAKYA
                     WVTLVPMLFVAATTLSACALIVEANYQLSQAPGQAVTAWANLVMTLVIMGSAVVVLVE
                     SFRRWWMILVEKKHPKALQKPVHPLPELPDYGCC"
BASE COUNT          555 a          895 c          959 g          509 t
ORIGIN      
        1 cgaggcgatg ttggaacaga tccaggcctc ggaccaatgg aactattacc aggccaaggg
       61 catcaaggcg gctgtgctgg gatcgaagat cgagatccta aagggcgaac gccgtgcgcc
      121 gtccaaggaa gacttggaga aaatggaaca atacaaggcc gaccaggaaa agatcagcgg
      181 ccaggcgaag gaatatgagc gctcctccga agagcgcctg ggacaccacg agagcttggc
      241 tttttccgtc actttcttcc aggtggccat cgctgtgggc gccatctcgg ccctggcccg
      301 ccgacgggcc ttttggctgg tcagtttggg tttcggggcg gtggggttgg tctttttggt
      361 cctggggttg ttctagccga ggggatacaa tggggccgtc cgttatcttg tcggtgggag
      421 gtggcgatgg aatctctttc gaaactcaac gcggaagcct acggggccct gcggtccgtg
      481 gcggggttct tgttcctgtt ccatggggcc cagaaactgt tcggcctttt cctggacccc
      541 agcctgatgc cccatttcgg cacccagatg tggttcgggg gcgtcattga gctggtgggg
      601 ggcgtcctca tcatggtggg gctctgcacc cgttgggcgg ccttcctggc cagcggcgag
      661 atggcggtgg cttatttcca gttccactgg aagttcgtct tcaccagcgc ctttttcccc
      721 ttggtgaacc acggggagat ggccgtcctc tactgcttcg tgttcttgtt catcgccacc
      781 cagggcccgg gcaagtggtc gctggacggg atgttgaaga agaaaaaatg agccgatcgg
      841 cccaataaaa aaggcccgcc cccggagggg gcgggccttt tgtcttttac cggatcactt
      901 gcccagcact tcgtccttca gggccttgat cacgcggaac ttcaccacgc gcttggcggc
      961 gatcttgatg gtctcgccgg tggccgggtt gcggcccatg cgggccttgc ggtccttcaa
     1021 ctggaacacg cccaagccgt cgagtttgat ctgcttttcc ttcttcaggg tcttgtagat
     1081 caggtcgttc aagcccagca tcagggtctg cacatccttc ttcttgaagc ccgtcttctc
     1141 cgccaaagcc gacagcattt cgcttttggt cacgagtgcg cctcccaggg attaaaaact
     1201 caaacgccgg tatttatcca aaagtccggg gtttttacaa ggaatttcgg gggccgaagc
     1261 ccccttttta taccgacggc tcccgttccc tagcagcagc cgtaatcggg cagctcgggc
     1321 aggggatgga cgggcttctg gagggccttg ggatgcttct tttcgaccag gatcatccac
     1381 cagcgccgga aggattccac caggacgacc accgcgctcc ccatgatgac gagggtcatg
     1441 accaaattgg cccaggccgt gaccgcctga ccgggcgcct gggacagctg atagttggcc
     1501 tccacgatca gggcgcaggc gctcaaggtg gtcgccgcca cgaagagcat gggcaccagg
     1561 gtcacccagg cgtattttgc ccgacccgtg ttgatgatgg cgctggtggc caccgccagg
     1621 gccacgctgg ccaagagctg gttggccgcg ccgaacatgg gccagatggt gcccaccgtg
     1681 ccggtggaca acaggtaggt ccaagcgccc accaccaggg cggtggaaag cagggtgccg
     1741 ggcagccact tgttgtcggc gaagcgcggc cacaggcggc cgccgtattc ctgcaccaga
     1801 aaacggccga tgcgcgtgcc cgcgtcgatg gtggtgagga tgaacagggc ctcgaacatg
     1861 atcatgaaat ggtagaagta ggccatcagg cccttcaggc cgggcagccc tgagaagatc
     1921 tgggcgaagc ccacggccaa cgacaccgcg ccgcccgggc gcccggccac gtcctcgccc
     1981 acggcctggt cgagcgcctg caggttcacc ggcgtcatcc ccaaggccgc gaacttgtcg
     2041 gcgggcacgt tgatggcgaa ataatcggcg gggaacagcg agcaggaggc cagcagggcc
     2101 acgatgccca ccaagccctc catcagcatg gcgccgtaac cgatgacccg ggcgtcgccc
     2161 tccttatcca gcatcttggg cgtggtgccc gaggccacca gggcatggaa gcccgagatg
     2221 gccccacagg cgatggtgat gaacatgaag gggaagagct tgccgtggat gatgggcccg
     2281 ccgccgtgga cgtaggaagt gaaggcgggc atctgcaggt cgggatgcac ccagaggatg
     2341 cccgccacca gcgcgccgat ggtccccagt ttcatgtagg tgctcaggta gtcccggggc
     2401 gtcaagagcg cccagatggg cagcacggac gccacgaagg cgtagagggc cagcgccacg
     2461 atgacgccct cgcgcgacag gcgcaggtaa gggtccagcg gcgaaccggg gaccaggttc
     2521 ccgaagatga cggcgaggaa gaccagggtc acgcccaccg cgctggcctc ggcgatgcgc
     2581 ccggggcgca ggcgcgacat ccaaaggccg gtcaagagcg cgatggggat ggtcatgccg
     2641 atcgaaaaga cgccccaggg gctgtccttg agcgcgttca ccaccgtgat gcccagaccc
     2701 gccagggcca cgatcacgat gatgaggatg gccagcgacc ccaccaggcc cgccaggggg
     2761 ctgatctcgt gccgggcgat ctcggccaac gacttgccct tgcgcctcac cgaagccgcc
     2821 aacaccatga aatcctgcac ggcgccggcc aggcagaccc cggccagcag ccagaggaag
     2881 cccgggcaaa aaccgaactg ggcggccagg accgggcc
//
LOCUS       DASTJJ010000354         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_906121, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000354 DASTJJ010000000
VERSION     DASTJJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906121"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFR02_04420"
     CDS             complement(<1..254)
                     /locus_tag="VFR02_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869730.1"
                     /translation="MLFATALLYFLLGDRRDAWVILAAVLPVLLVDVLLEARSKRALM
                     RLEQAAAPKARVMRDGRETLVPEAGLVRDDLLLLREGDRVP"
     gene            complement(408..713)
                     /locus_tag="VFR02_04425"
     CDS             complement(408..713)
                     /locus_tag="VFR02_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869731.1"
                     /translation="METFWDKLKWRVGKWIWAPPGEMPVTDPKDTRWVAPPALLPEGR
                     LPGRARRGRGDRRPFQPPIRPIPERGGALKREPHPRAEHKAPMRFKGKGTKTARRPA"
     gene            877..1149
                     /locus_tag="VFR02_04430"
     CDS             877..1149
                     /locus_tag="VFR02_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869732.1"
                     /translation="MRLETGRPAWVENANADNELTIDLGRYLRYGLEALPAVEDLLTE
                     LRPCQVLHLTSPREPVMLYEILRRKGYENFNEARNGVWHSYFRKCV"
     gene            1163..1333
                     /locus_tag="VFR02_04435"
     CDS             1163..1333
                     /locus_tag="VFR02_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869733.1"
                     /translation="MKKRMKGKRKARPPKAPARRRPADAGARQAPEVRRVLGQGEDEQ
                     ILENVFERDRED"
     gene            complement(1350..>1653)
                     /locus_tag="VFR02_04440"
     CDS             complement(1350..>1653)
                     /locus_tag="VFR02_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9869734.1"
                     /translation="ELRNVVRQGVLLAVGPALGPAQVGKLLGRSKTPRESQGPLEIPL
                     LPGQSLKQIAETAVEQAEKQAIASVLKSTGGNKSQAAKILKTDYKTLHVKVKKYGL"
BASE COUNT          276 a          581 c          551 g          245 t
ORIGIN      
        1 gggacccggt ccccctcccg cagcaacaaa aggtcgtcgc gcaccagtcc ggcctcgggc
       61 acgagcgttt ccctcccgtc ccgcatcacc cgcgccttgg gcgcggcggc ctgttccaac
      121 cgcatcaaag cccgcttgga ccgggcctcc agcagcacat ccaccaacaa cacgggaagg
      181 accgcggcca ggatcaccca agcgtcgcgc cggtcgccca ggaggaaata aagcagcgcc
      241 gtggcgaaaa gcatcagggc catcggatcc gccagcgccg ccaaacgccc gatccgatcc
      301 cgcatccggg tcggcgggga aaagacgttc gggccttccg ccgccagccg acgggccgcc
      361 tcccgggatg aaagcccccc tccacgcgct tccatgggcg ggccgcctca ggccgggcgg
      421 cgggccgtct tggtcccctt gcctttgaac cgcatggggg ccttgtgctc cgcccgggga
      481 tggggctccc gcttgagcgc cccgccccgc tccgggatgg ggcggatcgg gggctggaaa
      541 ggccgccgat ccccccggcc gcggcgggcc cttccgggca accggccctc gggcaacagg
      601 gcgggcggcg ccacccaacg ggtatccttc gggtccgtga cgggcatttc ccccgggggg
      661 gcccagatcc atttgcccac ccgccatttc aacttgtccc aaaaggtctc cataagcgcc
      721 tccctccgtt ggatctccat ggacccagcc taacccggac ccgggcgggc ccctatgagc
      781 cccgtcatac ggggatggcg cgcggggctt tatccttgga tcaggagacc catccatcgc
      841 cgcccccaag ggggacccgt cgtgaaggag gatgccatga ggctggaaac cggaagaccc
      901 gcctgggtgg agaacgccaa cgcggacaat gagttgacca tcgacttggg ccgctacctg
      961 cgctacggcc tggaggccct gcccgccgtg gaggacctgc tcaccgagct gcggccctgc
     1021 caggtgctgc acctgaccag tccgcgggag cccgtcatgc tctatgagat cctgcggcgc
     1081 aagggctacg agaatttcaa cgaggcccgc aacggggtct ggcactctta tttccgtaaa
     1141 tgcgtttgaa ccggagggga ccatgaaaaa gaggatgaag ggaaaaagga aggcgcgccc
     1201 gccgaaagcc ccggcgcggc gccgtccggc ggacgcaggg gcccggcagg cccctgaagt
     1261 gcggcgcgtc ctgggccagg gcgaggacga gcagatcctg gagaacgtct tcgagcgcga
     1321 ccgggaggat tgacccgcgc ggggccggcc taaaggccgt atttcttgac cttgacgtgg
     1381 agcgtcttgt agtcggtctt gaggatcttg gcggcttggc tcttgttgcc gccggtggac
     1441 ttcaacaccg aggcaatggc ctgcttttcg gcctgctcca cggcggtctc ggcgatctgc
     1501 ttgagggact gcccgggcaa cagggggatc tccaagggcc cctgggactc gcggggcgtc
     1561 ttggaccgtc ccaatagttt ccccacctgc gccggcccca gggcggggcc gacggcgagc
     1621 agcacgccct ggcgcaccac gttgcgtaat tcc
//
LOCUS       DASTJJ010000355         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_172753, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000355 DASTJJ010000000
VERSION     DASTJJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172753"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFR02_04445"
     CDS             <1..888
                     /locus_tag="VFR02_04445"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9869735.1"
                     /translation="VAYVLGGSTNIVLHLIAIAKAIGVKLTVDDFDRIGAKVPHLADL
                     KPSGRYVAADLDRIGGIPAVMKMLLAAKMLHGDCLTVTGKTVAENLRGAAPLSKNQPI
                     LHPLEKPLYPTAPMVILKGNLAPEGAVAKISGLKSVVHTGPARVFDGEEAAFAAIQKR
                     GIKAGDVVVIRYEGPKGGPGMREMLAITAALVGQGLGESVGLITDGRFSGGTHGLVVG
                     HIAPEAQVGGPIGLIKNGDKITIDAKKKKLELHLPAAELKKRRAKWKPRKNAYPKGVL
                     GKYVHLVSSASLGAVTDEF"
     gene            complement(1043..>1728)
                     /locus_tag="VFR02_04450"
     CDS             complement(1043..>1728)
                     /locus_tag="VFR02_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869736.1"
                     /translation="AAPAPVATPEPSDPSAKFDKFAFTLNGGFWAPNTTKQFNDDLEA
                     GTGDSSLDYLPGWFKFGMGFNWLTNGFGLKWNIQGSLQPNNYTTDWYYGGYYAGTTEQ
                     DTYILYFGSELEADICFDGVINKDNVTSFYIPLIVGVWSVDWNYQDSGGNALDYTNTT
                     TDFGTGIGVRGFDSSKFLWDFQLVYRWSARGNYLQDAYGDQISYVNGGSIDANVSGLD
                     LNCTIGFML"
BASE COUNT          320 a          552 c          566 g          290 t
ORIGIN      
        1 gtggcctacg tgctgggggg gtccaccaac atcgtgctgc acctcatcgc catcgccaag
       61 gccatcgggg tcaaactcac ggtggacgac ttcgaccgga tcggcgccaa ggtgccccac
      121 ctggcggacc tgaaaccctc cggccgttac gtggcggcgg acctggaccg catcgggggc
      181 atcccggcgg tcatgaagat gctgctggcc gccaagatgc tgcacggcga ctgcctgacc
      241 gtgacgggca aaacggtggc cgagaacctg aggggggcgg cccccctgtc caagaaccag
      301 cccatcctcc acccgctgga aaagcccctc tatcccacgg cgcccatggt catcctcaag
      361 ggcaacctgg cccccgaggg ggcggtcgcc aagatctcgg ggctcaagtc cgtggtgcac
      421 acgggcccgg cgcgggtctt tgacggcgag gaagcggctt tcgcggccat ccaaaagcgc
      481 gggatcaagg ccggggacgt ggtggtcatc cgctacgagg gccccaaagg ggggcccggc
      541 atgcgggaaa tgctggccat cacggcggcc ctggtgggcc agggcttggg cgagagcgtg
      601 gggctcatca ccgacggccg tttctcgggc ggcacccacg gcctggtggt ggggcacatc
      661 gcccccgagg cccaggtggg cggccccatc ggcctcatca agaacgggga caagatcacc
      721 atcgacgcca agaaaaagaa gttggaactg cacctccccg cggccgagct caagaaacgg
      781 cgcgccaagt ggaagccccg caagaacgcc tatcccaagg gggtcttggg caagtacgtc
      841 cacctggtct cctcggcgag cctgggcgcc gtgaccgacg aattctaaga tcgggccgat
      901 ggtgcttttc caagtccgcc ggccccatga cgccgatact gggaaccgcc gccccgaagg
      961 ggcggcctga ccggcgggat gcgggcaata aaaaaggccg tcccctttcc aagggacggc
     1021 ctttttcttt tgatcccccg tcttagagca tgaacccgat cgtgcagttg aggtccaacc
     1081 cactgacgtt ggcgtcgatg gaaccgccgt tcacgtagga gatctggtcg ccgtaggcgt
     1141 cctgcaggta attcccgcgc gcggaccagc ggtagaccag ttggaagtcc cagaggaact
     1201 tgctcgaatc gaaaccccgg acgccgatgc ccgtgccgaa gtccgtggtg gtgttggtgt
     1261 aatccagcgc gttcccgcct gaatcctggt agttccaatc caccgaccag acgcccacga
     1321 tgagcgggat atagaaggac gtgacgttgt ccttgttgat cacgccgtcg aagcagatgt
     1381 cggcctcgag ctcgctcccg aagtacagga tgtaggtgtc ttgttccgtg gtccccgcgt
     1441 aataaccccc gtagtaccaa tcggtggtgt agttgttggg ttgcagcgaa ccctggatgt
     1501 tccacttcag cccgaaacca ttggtgagcc agttgaagcc catgccgaac ttgaaccatc
     1561 cgggcaggta gtccagggac gaatcccccg tgcccgcctc caggtcgtcg ttgaactgct
     1621 tggtggtgtt gggggcccag aacccgccgt tcagcgtgaa ggcgaacttg tcgaacttgg
     1681 cgctggggtc gctcggctcc ggcgtggcca ccggcgcggg ggccgccg
//
LOCUS       DASTJJ010000356         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1150992, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000356 DASTJJ010000000
VERSION     DASTJJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150992"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFR02_04455"
     CDS             <1..202
                     /locus_tag="VFR02_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET9869737.1"
                     /translation="YFFILTTHTILAGIVPVLAILTLTRALKGQFDRHKKIARVTYPI
                     WLYVSVTGVVVYWMLFHLYAPA"
     gene            206..592
                     /locus_tag="VFR02_04460"
     CDS             206..592
                     /locus_tag="VFR02_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9869738.1"
                     /translation="MPPLRESGLILFASDYAASVAFYRDRVGLRVRFEKENLTCFQFG
                     GSYLLVEPRYGEEAVPAPNGNSPVWRLNVDSIDEVLAALRARGLAVEKMEFEWGTTVS
                     LRDPDGYMVEWCQWPESLYRDHPPLA"
     gene            606..1040
                     /locus_tag="VFR02_04465"
     CDS             606..1040
                     /locus_tag="VFR02_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9869739.1"
                     /translation="MANQSDPQAEQWVKEAATVAVVGMQDEAHSDRAAFRIPQMLQER
                     GLKLYPVNPKIQSSLGEKALPDLKSLPVKVDILDVFRRSEAVPELVEEILALPEDRRP
                     RGVWLQSGITHPDAEARLEAAGLKVVSDACLGVFAARVRPRA"
BASE COUNT          184 a          391 c          382 g          209 t
ORIGIN      
        1 gtatttcttc atcctgacca cccataccat cctggcgggc atcgtgccgg tcttggccat
       61 cctgaccctg acccgggcct tgaaggggca gttcgaccgc cacaagaaga tcgcccgcgt
      121 cacctacccc atctggctgt acgtgtcggt cacgggcgtg gtggtctatt ggatgctctt
      181 ccacctctac gccccggcct gagccttgcc gcccctccgg gagtcgggcc tcatcctttt
      241 cgcctcggat tacgccgcca gcgtggcctt ttaccgggac cgggtgggcc tgcgggtgcg
      301 ttttgagaag gaaaacctca cttgtttcca gttcgggggt tcttatcttt tagtggaacc
      361 ccgctacggc gaggaagcgg tgcccgcccc caacggcaac tcgccggtgt ggcggctgaa
      421 cgtggattcc atcgacgagg tcctcgccgc gctgcgcgcc cggggcctgg cggtggaaaa
      481 gatggagttc gagtggggga ccaccgtgag cttgcgggat cccgacggtt atatggtgga
      541 atggtgccag tggcccgagt ccctttaccg ggaccatccg cccctggcct gaaagcgagg
      601 gccccatggc caatcaaagc gatccccagg cggaacagtg ggtgaaggaa gccgccacgg
      661 tggcggtggt gggcatgcag gacgaggccc attcggaccg ggcggccttc cgcatccccc
      721 agatgctcca ggaacggggc ctgaaactgt atcccgtgaa ccccaagatc cagtcctccc
      781 tgggcgaaaa ggccctgccc gacctgaaga gcctgcccgt gaaggtggac atcctggacg
      841 tcttccgccg cagcgaggcc gtccccgagc tggtggagga gatcctggcc ctgccggagg
      901 accggcggcc ccgcggggtc tggctgcaaa gcggcatcac gcatcccgac gccgaggccc
      961 ggctggaagc ggccgggttg aaggtggtgt ccgacgcctg cctgggcgtc ttcgcggccc
     1021 gcgtgaggcc cagggcctga gggcggataa gcttgcgttc cacccggccg tcccttaccg
     1081 taaaggccat ccccggtccc gaggcccctg cccgatgaaa aaaacgatcc tggccctttg
     1141 gtggctgtcc ctggccgcgc ccctcg
//
LOCUS       DASTJJ010000357         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_906643, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000357 DASTJJ010000000
VERSION     DASTJJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906643"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1321
                     /gene="prfA"
                     /locus_tag="VFR02_04470"
     CDS             257..1321
                     /gene="prfA"
                     /locus_tag="VFR02_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9869740.1"
                     /translation="MLEPKFQAAEKRFEELSNRLSHPASIPNSAELQKLTIEHADLEP
                     LVRKIGEWRQWAREREEARAMLSGADPEMKALAQAEVPELDKKIEAAEKELKVMLLPK
                     DPDGAKNAILEIRAGTGGDEAALFVADLLRMYTRYLDRVGLTAELIDSSPTGIGGFKE
                     VIFEVRGKGAYGRLRFESGTHRVQRVPQTEAQGRIHTSAVTVAVLPEVDEVEVEIKPA
                     DLRIDTFCSSGAGGQSVNTTYSAVRITHLPTGLVVQCQDERSQLKNKAKAMKVLYARL
                     KEKADQEANAKTAQTRKNQVGSGDRSEKIRTYNYPQNRVTDHRIGLTLYQLDRVMDGA
                     LDEFVEALALAAQEEQLQNQ"
     gene            1439..2323
                     /gene="prmC"
                     /locus_tag="VFR02_04475"
     CDS             1439..2323
                     /gene="prmC"
                     /locus_tag="VFR02_04475"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9869741.1"
                     /translation="MAEKKSGEVWTPLKIIQWAVPFLAQKGVESPRFDAECLVAAALG
                     LDRLKVYLQFDRPLDPAELAVIRDYLSRRARREPVAYILGTREFYGKPFQVTPEVLIP
                     RPETEHLVEGALSFLKEWAPEEPQALDLGVGSGCIAVSLALNSPARVIGVDLSEGALR
                     VAEGNAQALGALVEWRHGSWFSALREGDPAQFHLIVSNPPYIAEPERGELAPDVLEYE
                     PQTALFSGISGLEAYAAIEPGLWDRLLPGGRAYLELHGTRLEPIRAVFGGRPWETEVI
                     PDLQGLPRVLVLKKSRNS"
     gene            2441..>3084
                     /locus_tag="VFR02_04480"
     CDS             2441..>3084
                     /locus_tag="VFR02_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9869742.1"
                     /translation="MKKVKFGRRERIDFSRIPSIMDMPDLIEFQRKSYDAFLQKDTPM
                     EQRTDLGLHSVFTSIFPIWDFNEVSSLEFVSYTLLEPKYNVRECQERGMTYSVPMKVT
                     LRLVIREEDPETNTKKVKDIREQEVFFCDLPMMTERGTFMINGAERVIVSQLHRSPGV
                     TFDEDDGKLSVSGKKLYMTSVIPYHGSWLEFEFDVNGVLYARIDRKRKFVATTLL"
BASE COUNT          623 a          963 c          968 g          530 t
ORIGIN      
        1 ggagaagttc cagaagaaga gcgccaccgc cgccaacgtg caggccgagg tcaaggcccg
       61 cgaggaagcc aaggccgtgg ccgccgccgc caagaaggcc aagcgccagg ccgcgccgcc
      121 cccggcctcc ccgtcgatgg acgcgccgtc gcaaaactag cttttttccg aacaaccgaa
      181 aaccccagga cgcgccgccg cggcctgggg tttttttcgt tctccctttt cccagcctcc
      241 cccataggaa accgccatgc tggaaccaaa atttcaagcc gccgagaagc ggttcgagga
      301 actgtcgaac cgcttgagcc atccggccag catccccaac tcggccgagc tgcagaagct
      361 caccatcgag cacgccgacc tggaacccct ggtgcgcaag atcggcgaat ggcgccaatg
      421 ggcccgggag cgggaggagg cccgggccat gctttcgggc gccgaccccg agatgaaggc
      481 gctggcccaa gccgaggtcc ccgagttgga caagaagatc gaggccgccg agaaggaact
      541 caaggtgatg ctgctgccca aggacccgga cggcgccaag aacgccatcc tggagatacg
      601 cgcggggacc ggcggggacg aggccgccct gttcgtggcc gacctgctgc gcatgtacac
      661 ccgctacctg gaccgggtgg ggctgacggc ggagctcatc gactccagcc cgacgggcat
      721 cggcgggttc aaggaggtca tcttcgaggt gcgggggaag ggcgcctacg gccggttgcg
      781 tttcgagagc ggcacccacc gggtccaacg ggtgccccag accgaggccc aggggcgcat
      841 ccatacctcg gcggtcacgg tggccgtgct gcccgaggtg gacgaggtgg aggtggagat
      901 caaacccgcg gacctgcgca tcgacacctt ctgctcctcg ggcgcggggg ggcaaagcgt
      961 gaacaccact tattcagcgg tgcgcatcac ccacctgccc accgggttgg tggtgcagtg
     1021 ccaggacgag cggtcccagc tcaagaacaa ggccaaggcc atgaaggtgc tctacgcccg
     1081 gctcaaggaa aaagccgacc aggaagccaa cgccaagacc gcccagacgc gaaagaacca
     1141 agtgggcagc ggggaccgca gcgagaagat ccggacctac aattacccgc agaaccgggt
     1201 gacggaccac cgcatcggct tgaccctcta ccagttggac cgggtcatgg acggcgccct
     1261 ggacgagttc gtggaagccc tggccctcgc cgcccaggaa gaacaactgc agaaccaatg
     1321 atcagaccat tttcaccgca aagacgcaaa ggcgccaagg aaggcgggaa gaaaataaaa
     1381 ggaccttgtt ttttccttct tcgcgttttg gcgtctttgc ggtaaagagg tggttcggat
     1441 ggcggaaaag aaaagcggtg aggtgtggac cccgctcaag atcatccagt gggccgtgcc
     1501 cttcctcgcc caaaaggggg tggagagccc ccgattcgac gccgaatgcc tggtggcggc
     1561 cgccttgggg ttggaccggc tcaaggtcta cctgcagttc gaccgtcccc tggacccggc
     1621 cgaattggcc gtcatccggg attacctgtc ccggcgggcc cggcgggagc ccgtggccta
     1681 tatcctgggc acccgcgaat tctatggcaa acccttccag gtgaccccgg aggtcttgat
     1741 cccccggcct gaaaccgagc acttggtgga aggggccctt tctttcctga aggaatgggc
     1801 tcccgaggag ccccaggcct tggacctggg ggtgggaagc ggctgcatcg cggtttccct
     1861 ggccttgaac agccccgccc gggtcattgg ggtggacctg tcggaggggg ccttgcgggt
     1921 ggccgagggc aatgcccagg ctttgggggc cctggtggaa tggcgccatg ggtcgtggtt
     1981 ttcagccctc cgggaggggg atccggccca gttccatctg atcgtgagca atccccctta
     2041 tattgcggag cctgaaaggg gggaattggc ccccgatgtg ctggaatatg agccgcaaac
     2101 ggccctcttt tcagggataa gcggcttgga ggcctatgca gcgatcgaac ccgggctctg
     2161 ggatcggctt ttaccggggg ggagggccta cctggaactg cacgggaccc gcctggagcc
     2221 cattcgggcg gtctttgggg gtaggccttg ggagacggaa gtgatccccg acctgcaggg
     2281 cctgccgaga gtgcttgttt tgaaaaagtc aaggaattct tgactttccc gtgaactgct
     2341 cactataatc ccacctctta cgccctaaac gagaaaaatc ccattttctc gttttttttg
     2401 tctcgtgggc ttggcgccat cttgtctgga ggagtccctg gtgaaaaaag tgaaattcgg
     2461 ccgccgtgaa cgcattgatt tcagccgcat cccgtccatc atggacatgc ccgacctgat
     2521 cgaattccaa cgcaaatcct atgacgcctt cctccaaaaa gacaccccga tggaacagcg
     2581 caccgacctg ggcctgcact cggtcttcac cagcatcttc ccgatctggg acttcaacga
     2641 agtttcttcc ttagagttcg tctcctacac gttgctggaa cccaagtaca acgtgcgcga
     2701 atgccaggaa cggggcatga cctattcggt gcccatgaag gtgaccctgc gcctggtcat
     2761 ccgcgaggaa gaccccgaga ccaacaccaa gaaggtgaag gacatccgcg agcaggaggt
     2821 cttcttctgc gacctgccca tgatgaccga gcgcggcacc ttcatgatca acggcgccga
     2881 gcgcgtgatc gtgtcccagc tgcaccgttc ccccggcgtc acctttgacg aggatgacgg
     2941 caaactgtcc gtctccggca agaagcttta catgaccagc gtgatcccct accacgggtc
     3001 ctggctggag ttcgagttcg acgtgaacgg cgtgttgtac gcccgcatcg accgcaagcg
     3061 caagttcgtg gccaccacgc tgct
//
LOCUS       DASTJJ010000358         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1762182, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000358 DASTJJ010000000
VERSION     DASTJJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762182"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1035
                     /locus_tag="VFR02_04485"
     CDS             <1..>1035
                     /locus_tag="VFR02_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9869743.1"
                     /translation="AALGPTVSEVGVHLPAIFLVGFIPMLLVALGYRELNNAMPDTGT
                     SFTWATRAFGPWLGWMGGWGLIAATIIVLSNLAAVAVDFFYLMLAQIFRNPSLADLTR
                     VLPLNIATTLVFIAGACWISYRGMETTKAFQYILVAFQLLVLGWFAIAAFAHVANGTA
                     FDATAISADWFNPFAVGSFSAFAAGVSLSIFIFWGWDVTLTMNEETKNPEKTPGRAAT
                     VTVVVIVVIYMVVSLATLSFAGIGTTGLGAGNPANQGSIFAVLAGPVMGPFAILMSLA
                     ILSSSAASLQSTFVSPARTLLSMGHYRALPKSFGRISPAHKSPGYATVAAAVAAAGFY
                     VITRSLSENAL"
BASE COUNT          134 a          376 c          317 g          208 t
ORIGIN      
        1 cgccgccctg ggccccacgg tgtccgaggt gggcgtgcac ctgccggcca tcttcctggt
       61 cggcttcatc cccatgctgc tcgtggcctt gggctaccgc gaactgaaca atgccatgcc
      121 cgacaccggc acctccttta cctgggcgac gcgcgccttc gggccgtggc tgggctggat
      181 gggcggctgg ggcctgattg cggccacgat cattgtcctg tccaacctgg cggccgtggc
      241 ggtggacttc ttctacctga tgctcgcgca aattttccgg aacccgtcgc tggcggacct
      301 gacccgcgtg ctgcccctga acattgccac cacgttggtg ttcattgccg gggcctgctg
      361 gatttcctac cgcggcatgg agaccaccaa ggcgttccag tacatcctgg tggcgttcca
      421 gctgctggtg ctgggctggt ttgccattgc ggccttcgcc cacgtcgcca acggcacggc
      481 ctttgacgca acggccatct cggcggactg gttcaacccc tttgccgtgg gctcgttctc
      541 cgcgttcgcg gccggcgtct cgctgtccat cttcattttc tggggctggg acgtcacctt
      601 gaccatgaac gaggaaacga agaacccgga aaagaccccg ggccgcgcgg ccaccgtcac
      661 ggtggtggtc atcgtggtga tctacatggt ggtttccctg gccaccctgt ccttcgccgg
      721 cattggcacc accggcctgg gcgccggcaa cccggcaaac cagggcagca tcttcgccgt
      781 gctggccggc ccggtcatgg gaccgttcgc catcctgatg tcgctggcca tcctcagctc
      841 gtccgcggcg tcgctgcagt ccaccttcgt gtccccggcc aggacgctgc tgtccatggg
      901 ccactaccgg gccctgccca agagcttcgg gcggatcagc cccgcccaca agtccccggg
      961 ctacgccacc gtcgcggccg ccgtcgccgc cgcgggcttc tatgtcatca cccggtcgct
     1021 gtccgagaac gccct
//
LOCUS       DASTJJ010000359         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_295890, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000359 DASTJJ010000000
VERSION     DASTJJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295890"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFR02_04490"
     CDS             complement(<1..870)
                     /locus_tag="VFR02_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9869744.1"
                     /translation="MIRKSLLALALGFACSAASAATVEDWGAIAPGKDVGGFADSNGS
                     KCDVAQAAGPQGGQKSVQISATLVQGGYAGIWHNLAAPADLSKSANLVFQAKSTMPGE
                     LQLDIQDAHNVSYTVNFQVPSASWTQVTIPISSFKLNPYYQPPEAVKGSPEDLSQVGK
                     IGFGTHIVGTAVVNIGPITTEGTGSPAAAAAAPAPIVATGAPMVIQDFSTDDPKAGGT
                     FADSNGSSLAYSVKANSNKPGAKFLVINFNEVAGGYCGMFYHTGNNWDGQDWSGGKAV
                     QMMVYSKVPVVLGF"
     gene            complement(903..1085)
                     /locus_tag="VFR02_04495"
     CDS             complement(903..1085)
                     /locus_tag="VFR02_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869745.1"
                     /translation="MKNFSLPRLGLLLWFANFSFFCVVDNYLIGGRHSIFTFLVYSAV
                     CVAVELLVFGWLEDVL"
     gene            complement(1106..1714)
                     /gene="ruvA"
                     /locus_tag="VFR02_04500"
     CDS             complement(1106..1714)
                     /gene="ruvA"
                     /locus_tag="VFR02_04500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9869746.1"
                     /translation="MISFLQGILASKSPERVEVDVNGVGYELAVSQKTYQSLPAPGQT
                     LLLKAYLHVREDVMQLYGFAGDAEKQAFLLLISVSGVGPKAALGILSSFTPEQLFRAV
                     AQGVVPALRTAPGVGQKTAQHLVLELREKVTKLSGAGVFSGEEPVGAGAQNLGDAIQA
                     MLALGYSAAEARRAVLTAAQSLGRDPGVEDILKLSLKTVAGS"
     gene            complement(1858..2421)
                     /locus_tag="VFR02_04505"
     CDS             complement(1858..2421)
                     /locus_tag="VFR02_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869747.1"
                     /translation="MKCKNCRNKLFENARYCPECGYPVDEKFNVTPEDFLKDTVNKGP
                     TDKLSPDSGKPMLALEHQGHDYLWDEESMGVWIDKSILEGIDPEAAQVVFENLSAEDQ
                     KYFNQAKEHIKLKASGMKSPLTDNELDEHHYKALVIDEDFESKGIWLDQNVISFWYAA
                     TQNKEAPALTQVEELLHILVHLKFLED"
     gene            complement(2460..>2873)
                     /gene="ruvC"
                     /locus_tag="VFR02_04510"
     CDS             complement(2460..>2873)
                     /gene="ruvC"
                     /locus_tag="VFR02_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9869748.1"
                     /translation="TLTTPAGEAVEKRLVRLFEGLRGLVEAHRPQVLALEILFFSKNV
                     TTGIAVGQARGVAVLVAGLAGIPVAEYRPAEVKQAVTGYGLADKQQVQKMVKLLLGLT
                     ETPKPDDTADALAVALTHAQGGGGRWPAVGKDDRK"
BASE COUNT          518 a          969 c          853 g          533 t
ORIGIN      
        1 gaagcccagg accaccggca ccttggaata gaccatcatc tggacggcct tgccgccgct
       61 ccagtcctgg ccgtcccagt tgttgcccgt gtggtagaac atgccgcaat agccgcccgc
      121 cacctcgttg aaattgatga ccaggaactt ggcgccgggc ttgttcgagt tggccttcac
      181 cgaataggcc aggctggaac cgttggagtc ggcgaaagtg ccgcccgcct tggggtcgtc
      241 ggtggagaaa tcctggatga ccatcggggc gccggtggcg acgatcggcg ccggggcggc
      301 cgcggccgcc gcgggcgaac ccgtgccctc ggtggtgatg ggcccgatgt tgaccaccgc
      361 cgtgcccacg atatgggtgc cgaaaccgat cttgcccacc tggctcaggt cctccgggct
      421 gcccttgacg gcttccgggg gctggtaata ggggttcaac ttgaaggagg agatcgggat
      481 ggtgacctgg gtccaggaag cggaaggcac ctggaaattg accgtgtagc tcacgttgtg
      541 cgcgtcctgg atgtccaact gcagctcacc cggcatggtg gacttggcct ggaagaccag
      601 gttggccgac ttcgacaggt ccgccggcgc ggccaggttg tgccagatgc ccgcgtagcc
      661 gccctgcacc agggtggcgc tgatctgcac cgacttctgg ccgccttggg gtcccgcagc
      721 ctgggccacg tcgcacttgg agccattgga atcggcgaag ccccccacgt ccttgcccgg
      781 cgcgatggcg ccccagtcct ccaccgtggc ggcggaagcg gcggaacaag cgaatcccaa
      841 ggccaaagcc aacaacgatt tgcggatcat gcgaccctcc tggtcaggat aacaaccgac
      901 ggctacagca cgtcctccaa ccacccgaaa acgagcaatt cgacggctac gcagaccgcc
      961 gagtagacca ggaaggtgaa gatggaatgg cgccccccga tcaagtaatt gtccacgacg
     1021 caaaaaaagg agaaattcgc gaaccaaagc aaaagaccca ggcggggcag actgaaattt
     1081 ttcatccttc actcctcggc cgggctcagg acccggccac tgttttcaag ctcaacttca
     1141 ggatatcctc gacccccggg tcccggccca gggactgggc cgccgtcaac accgcccggc
     1201 gtgcctcggc ggccgaatag cccagggcca gcatggcctg gatggcgtcc cccaggttct
     1261 gggcgcccgc gccgacgggc tcctcaccgg aaaatacgcc ggccccggac agtttggtta
     1321 ccttctcccg cagttccagc accaggtgct gggccgtctt ctgccccacg ccgggggccg
     1381 tccgcagggc cgggacgacg ccctgggcca cggcccggaa aagctgctcg ggcgtgaagg
     1441 acgacaggat gcccaaggcc gccttggggc ccacccccga gaccgagatc aacagcagga
     1501 aggcctgttt ttccgcgtcc cccgcgaaac cgtagagctg catcacgtct tcgcgcacat
     1561 gcaggtaggc cttcagcagc aaggtctgcc ccggcgccgg aagcgactgg taggtcttct
     1621 gggacaccgc cagttcatac cccacgccgt tgacgtccac ctccacccgc tcgggcgatt
     1681 tggaggccag gataccctgt aaaaaggaaa tcaaatcacg aacccctttt tcaccaccaa
     1741 gacaccaagt tcaccaagaa agacccggca caaaatttca gggtcccatc cgaccttggt
     1801 gcctttgtgc cttggtggtg aggatacgcc tcggtcttta accccgttct atccggctca
     1861 atcttccagg aacttcaggt gcaccaggat gtggagcagc tcctccacct gggtcaaggc
     1921 cggcgcttcc ttgttctgcg tggccgcgta ccagaaggaa atgacgttct ggtccagcca
     1981 gatgcccttg gactcgaaat cctcgtcgat gaccagggcc ttgtaatgat gctcgtccag
     2041 ctcattgtcc gtcaaggggc tcttcatgcc cgaggccttg agcttgatgt gctccttggc
     2101 ctggttgaag tatttctggt cctccgcgga gaggttctcg aagaccactt gggcggcctc
     2161 ggggtcgatg ccctccagga tgctcttgtc gatccagacg cccatgctct cctcgtccca
     2221 aaggtagtca tggccctggt gctccaaggc cagcatgggc ttgccgctgt ccgggctcag
     2281 cttgtccgtg ggccccttgt tgaccgtgtc cttcaggaaa tcctccgggg tgacgttgaa
     2341 cttctcatcc accggatagc cgcactcggg gcaataacgg gcgttctcga aaagcttgtt
     2401 gcggcaattc ttgcatttca tcggtctctc ctcgcctctc tatgaatccg gatgcagcgt
     2461 catttccggt cgtccttccc aactgccggc caccggccgc cgcccccttg ggcgtgcgtc
     2521 agggccaccg ccaaagcgtc cgccgtatcg tcgggcttgg gcgtttcggt caaccccagg
     2581 aggagcttca ccattttttg cacctgctgc ttgtccgcca gtccgtaacc cgtcaccgcc
     2641 tgcttcacct cggcggggcg gtactccgcc accgggatgc ccgccaaccc cgccaccagg
     2701 accgccacgc cccgggcctg ccccaccgcg atgccggtgg tcacgttctt ggagaagaaa
     2761 aggatctcca gggccagcac ctgcggccgg tgggcctcca ccaggccccg caatccctcg
     2821 aacaggcgca ccagcctttt ttccaccgct tcccccgcgg gggtcgtgag ggt
//
LOCUS       DASTJJ010000360         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1762626, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000360 DASTJJ010000000
VERSION     DASTJJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762626"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFR02_04515"
     CDS             <1..852
                     /locus_tag="VFR02_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9869749.1"
                     /translation="LLGPNGAGKTTTMRVLTTSLPPSAGKVTVGGFDVVEASHQVRRR
                     IGYLPEVPPIYHEMTLMAYLRFVADLKEIDKSKKADRIGEVLEMLDLTDMSKRLIGHL
                     SLGYRQRVGLAQALLHDPEVLVLDEPTRGLDPKQILEIRKLIKSLSGRKTIILSTHIL
                     PEVSSTCDNVVIIDHGKVVAADSVEGLSQRMAGGKTLELEVKGLAAEVEKALAKSEGV
                     AGVRALGRETRGLSRFEVTTDGSTEAREGLFQCVVRNKWVLYQLNPVGMSLEDVFVKL
                     TTKEGAA"
     gene            849..>1288
                     /locus_tag="VFR02_04520"
     CDS             849..>1288
                     /locus_tag="VFR02_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9869750.1"
                     /translation="MSWTKVWALAKKEIGYYFASPVGYFILAAFFFATGLFFSLIVLG
                     TRQASMVPVFQNTVILLLFLAPGITMRLWSEEEKNGTAELLRTSPLSLWEIVLGKYLG
                     VSAFFGVMLASTLVYLLVLVADGNPDAGPVLTGYLGYILVALAF"
BASE COUNT          215 a          420 c          415 g          238 t
ORIGIN      
        1 ctgctgggcc ccaacggcgc cggcaagacc accaccatgc gggtcctgac cacgtccctg
       61 ccgccgtccg ccggcaaggt gaccgtcggg ggtttcgacg tggtggaagc ctcccatcag
      121 gtccgccgcc gcatcggtta cctccccgaa gtgcccccca tctatcatga gatgaccttg
      181 atggcctacc tgcggttcgt ggccgacctc aaggagatcg acaagtcgaa aaaggccgac
      241 cgcatcggcg aagtgttgga gatgctcgac ctcaccgaca tgtccaagcg cctgatcggc
      301 cacctgtcgc tgggttaccg ccagcgcgtg ggcttggccc aggccctgct gcacgacccc
      361 gaagtgctgg tgctggacga acccacccgc gggctcgacc ccaagcagat cctggagatc
      421 cgcaagctga tcaagtcgct cagcggccga aagaccatca tcctgtccac ccatatcctg
      481 cccgaggtct cctcgacctg cgacaacgtg gtcatcatcg accatggcaa ggtcgtggcg
      541 gccgattcgg tggagggcct gtcccagcgc atggccgggg gcaagaccct ggagctggaa
      601 gtgaagggcc tcgccgccga ggtggaaaag gccctggcca agagcgaggg cgtggccggg
      661 gtccgggccc tgggccggga gacccggggg ctcagccgtt tcgaggtgac caccgacggc
      721 tccacggagg cccgggaagg cctgttccag tgcgtggtcc ggaacaagtg ggtgttgtac
      781 cagctgaatc cggtcggcat gagcctggag gacgtcttcg tgaaactcac caccaaggag
      841 ggcgcggcat gagctggacc aaggtctggg ccctggccaa aaaagagatc ggctattact
      901 tcgcgtcccc cgtgggttat ttcatcctgg cggccttctt cttcgccacc gggctttttt
      961 tctccctgat cgtcctgggg acccggcagg ccagcatggt gccggtcttc cagaacaccg
     1021 tgatcctgct gctgttcctg gcgcccggca tcaccatgcg cctctggtcc gaagaggaaa
     1081 agaacggcac ggccgagttg ttgcgtacct cgcccttgag cctgtgggag atcgtgttgg
     1141 gcaagtacct gggcgtcagc gcctttttcg gcgtcatgct ggcctccacg ctcgtctact
     1201 tgctggtgct ggtggccgac ggcaacccgg acgcggggcc ggtgctgacc ggctacctgg
     1261 gctatatcct ggtggccctg gctttctt
//
LOCUS       DASTJJ010000361         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1396687, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000361 DASTJJ010000000
VERSION     DASTJJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396687"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFR02_04525"
     CDS             complement(<1..738)
                     /locus_tag="VFR02_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9869751.1"
                     /translation="MTPDPRTGKLIFSQVQNLLKLLQSGKDFSPEMLVLKSILEETRE
                     GVLIAGGDGRFLYMNAVMRRLHGNHELSDLDPARWAETYGLYKADGSSLYDPQDLPLA
                     RALRGETVEDQRLFVRNEWTPGTLLSARGGPLRDEKGEVKGGLVFLNDITREDAERVR
                     AQRLADLVDFSPISIIETTSEARIVSWNAGAERVYGYKAEEIIGKFYSRLVPLDKLPE
                     VQKQVLRLLKGELVLEHETVRVRKDGES"
     gene            complement(865..1596)
                     /locus_tag="VFR02_04530"
     CDS             complement(865..1596)
                     /locus_tag="VFR02_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET9869752.1"
                     /translation="MSKIQMKVYRGNAEGGQLKDYEVEVDEGMVVLDAIHRLQATQAG
                     DLACRWNCKAGKCGSCSAEINGKPRLMCMTRLNDLPPGQPVTVAPMKTFPLMKDLATD
                     VSWNYEVNKTIPPFTPREGADWHWEQEDVDRVQEFRKCIECFLCQNTCHVLRDHDKKE
                     AFAGPRFFARIASLEMHPIDGVDRTGLLKDKHGLGYCNITKCCTEVCPESIHITDNAI
                     IPLKERVVDKYYDPIKMLWRKVTGK"
BASE COUNT          267 a          520 c          536 g          331 t
ORIGIN      
        1 gctttcgccg tccttgcgca cgcggacggt ctcatgttcc agcaccaatt cgcccttcaa
       61 gagccgcagg acctgttttt gcacttccgg caatttatcc agggggacga gacggctgta
      121 gaacttcccg atgatctcct cggccttgta gccgtagacc cgctccgcgc ccgcgttcca
      181 gctcacaatg cgcgcctcgg acgtggtctc aatgatggag atgggggaaa aatccaccag
      241 gtcggccaga cgctgggccc ttacccgctc ggcgtcctcg cgggtaatgt cgttgaggaa
      301 gaccaggccg cccttgacct cgcccttttc gtcccgcagg gggccgcccc gggcgctcag
      361 gagcgtgccc ggggtccatt cattgcgcac gaagaggcgc tggtcctcca cggtctcgcc
      421 gcgcagggcc cgggccaggg gaaggtcttg gggatcgtaa agggaggacc catcggcctt
      481 gtagaggccg taggtctcag cccagcgggc cgggtccagg tcggaaagct catggttgcc
      541 atggagccgg cgcatcacgg cgttcatgta gaggaaccgg ccgtcgcccc cggcgatgag
      601 gacgccttcc cgggtctctt ccaagatgga cttgagcacc agcatctcgg gggaaaagtc
      661 cttgccgctt tggaggagtt tcaggaggtt ctggacctgg gaaaagatca gcttgccggt
      721 gcggggatcg ggtgtcacgc aaaccgcctt ggacggatgg agtgtccaag aatggtagca
      781 caggcggggc gggggcgggt tacgccatgg ccccggccgc cttcgcaaat gtgcttgttt
      841 tttggccgct taaccctaaa gcggtcactt ccccgtcacc ttgcgccaga gcatcttgat
      901 ggggtcgtaa tatttgtcca cgacccgttc cttcaagggg atgatggcgt tgtccgtgat
      961 gtggatggac tcggggcaga cctcggtgca gcacttggtg atgttgcagt agcccaggcc
     1021 gtgcttgtcc ttcaaaaggc ccgtgcggtc caccccgtcg atggggtgca tctccagcga
     1081 ggcgatgcgg gcgaagaagc ggggcccggc gaaggcctcc ttcttgtcgt ggtcgcgcag
     1141 cacgtggcag gtgttctggc agaggaagca ctcgatgcac ttgcggaatt cctgtacccg
     1201 gtccacgtcc tcctgctccc agtgccagtc cgcgccctcc cggggcgtga agggcgggat
     1261 ggtcttattg acctcgtagt tccaggacac gtcggtggcc aggtccttca tcagggggaa
     1321 ggtcttcatg ggcgccacgg tgacgggctg gcccggcggc aggtcgttca ggcgggtcat
     1381 gcacatgagc cgcggcttgc cgttgatctc ggccgagcag gaaccgcact tgcccgcctt
     1441 gcagttccaa cggcaggcca ggtcgccggc ctgggtggcc tgcaggcggt ggatggcgtc
     1501 cagcaccacc atcccctcgt ccacctcgac ctcatagtcc ttcaattggc cgccctcggc
     1561 gttgccgcgg tagaccttca tttggatctt cgacatggcc ttctccttga tgaaccgacc
     1621 tgcatccccc cgcttgaagc cccgacccga cacc
//
LOCUS       DASTJJ010000362         1796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1396714, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000362 DASTJJ010000000
VERSION     DASTJJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396714"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..169
                     /locus_tag="VFR02_04535"
     tRNA            97..169
                     /locus_tag="VFR02_04535"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Thr,seq:cgt)
     gene            174..248
                     /locus_tag="VFR02_04540"
     tRNA            174..248
                     /locus_tag="VFR02_04540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Leu,seq:gag)
     gene            253..327
                     /locus_tag="VFR02_04545"
     tRNA            253..327
                     /locus_tag="VFR02_04545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Thr,seq:tgt)
     gene            415..488
                     /locus_tag="VFR02_04550"
     tRNA            415..488
                     /locus_tag="VFR02_04550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:449..451,aa:Thr,seq:ggt)
     gene            574..646
                     /locus_tag="VFR02_04555"
     tRNA            574..646
                     /locus_tag="VFR02_04555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:607..609,aa:Gly,seq:gcc)
     gene            774..848
                     /locus_tag="VFR02_04560"
     tRNA            774..848
                     /locus_tag="VFR02_04560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:809..811,aa:Leu,seq:cag)
     gene            981..1071
                     /locus_tag="VFR02_04565"
     tRNA            981..1071
                     /locus_tag="VFR02_04565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1016..1018,aa:Ser,seq:gct)
     gene            1155..1239
                     /locus_tag="VFR02_04570"
     tRNA            1155..1239
                     /locus_tag="VFR02_04570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1189..1191,aa:Ser,seq:gga)
     gene            1244..1337
                     /locus_tag="VFR02_04575"
     tRNA            1244..1337
                     /locus_tag="VFR02_04575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1283..1285,aa:Ser,seq:cga)
     gene            1491..1682
                     /locus_tag="VFR02_04580"
     CDS             1491..1682
                     /locus_tag="VFR02_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869753.1"
                     /translation="MDVVQVDLRSGATRLTCWVEPRVRPGDRITLKNAGEPERLWDVL
                     RVGERRPAGSINRGWNNNI"
     gene            1704..1777
                     /locus_tag="VFR02_04585"
     tRNA            1704..1777
                     /locus_tag="VFR02_04585"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Ile,seq:gat)
BASE COUNT          282 a          584 c          644 g          286 t
ORIGIN      
        1 atcctgcctc cccaggtcat ccctggggag tagctcagtg gcagagcgcc ggcacccccg
       61 ggccgtattg gggtgcacca ccggcccggg ggccgtgccc gagtagctca gtcggcagag
      121 cgctcccctc gtaaggaaga ggtcgtcggt tcgatcccgg cctcgggctc tgcgtcccgg
      181 tagaccaacc ggcaggagtc gccaccctga gacggtggaa tgtctgggtt cgaatcccag
      241 tcgggacacg atgccctcgt acctcaaatg gcgagaggga cccccctgta aggggtctac
      301 atgcaggttc aagtcctgtc gagggctctc gatggctcgt ggggtaatcg gcagcccgcg
      361 gggtcttggc ccccgtagtc caggttcgat ccctggggag ccagcgcagt gcacgcccgg
      421 atagctcagt cggtcagagc gcgtccttgg taaggacgag gtcggcggtt cgaagccgcc
      481 ttcgggctct gggtccgcga ggacctgttc ccgcccctcc gggggcggat gtgcggtgta
      541 agtcctggcc tgttcgctgg gccgggcatc catgtggctg tggcgcagca ggtagcgcgg
      601 caccttgcca aggtgcaggc cgcgggttcg atccccgtca gccactcaat ccaggcagac
      661 cggccgtccg cggcggtacg ggcagtccta ccggaggacg ctgcctggct cttcacccga
      721 tcccccgtga cgggtcctgg ccgtgaggcg gagagcaagg cccgggggag caagtcccgg
      781 tagaccaatc ggcaggagtc acacggctca gacccgtgga tgtgtgggtt cgaatcccac
      841 ctgggacacg gcgctctcat cgagcgcaag ggcggcatgg ccaaagcctg gtaccgggcc
      901 tccgcgccgc accgaccctg acgcctgcgg ctggcgtcca tcgcgcgagg acgctcgcga
      961 gggctgaaac gccgcgacca ggaggggtgg catgagtggc tgatggcgct gccctgctaa
     1021 ggcagtaggg gccggaaggt tccctcgtgg gttcgaatcc cacctcctcc gcaatgcccg
     1081 gcttctggtc cacgtacccg ggcagtccga gcgctcgggc agcagggccg agacctgcct
     1141 aacgtgggcc gcctggaggg ttcgcctagc ggactatggc gctgcaccgg aaatgcagtt
     1201 ggggtaacgc cctcgggggt tcgaatccct caccctccgc cctggggagg taagccgacg
     1261 gcgggcctac cggccgcggt ctcgaaaacc gttggggggc gcgagtcccc gtgtgggttc
     1321 gaaccccacc ctctccgctt aaccaggaag gatgtcggcc atggacagct ttccggtagg
     1381 cagctcgatg aacccgaccc ctgactgcgg cggcccccgc aagccgccgg ctgccaccga
     1441 gctgtggtgg tgtaaccggc acggggagtt ccacgaggag ggagctggcg atggacgtgg
     1501 tccaggtgga cctgagatcg ggcgcgacga ggctcacctg ctgggttgag cccagggtcc
     1561 ggcctggcga ccggatcacc ttgaagaacg cgggcgagcc cgagcggctc tgggatgtcc
     1621 tgcgggtcgg cgagcgccgc ccggccggga gcatcaaccg cggctggaac aacaacatct
     1681 gatccccggc ccggccgggg cgagggcctg tagctcagat ggttagagcg cccggctgat
     1741 aaccgggagg tcggtggttc aattccacct gggcctacga gcgcgttcct gggcga
//
LOCUS       DASTJJ010000363         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_662818, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000363 DASTJJ010000000
VERSION     DASTJJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662818"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..>2039)
                     /locus_tag="VFR02_04590"
     CDS             complement(24..>2039)
                     /locus_tag="VFR02_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9869754.1"
                     /translation="KRSGGGAEGPDRLFFAGTRVLTGQGLGQVSRTGPRSRLGAIARL
                     ASQGLESPSPLQEKIAALTRRLVLAAVLLVAALFLVSLATGAGPAAAFLSAVSLGMAA
                     IPEEFSLVLAVFLSLGAFRLSRRGVLVKRLAAVETLGSTTVICLDKTGTLTEGRFTLG
                     PPVPLAPGVDGRGLLEAALWACEASPADPMEQAILEAARRSGTDPDRLQGGWRLVHDY
                     DLDPVGKRMAHVWRRAGGEDVRVAAKGSLEGILAVCELAPGERERAESENARLAGGGG
                     RVLAVAGREGGDCTGDRSRDERGLRLYGLLAFRDPLRPDVPGAVRACQGAGVKLKLVT
                     GDHPLTARAVADAAGIAEGSGGPWTGTDLAQWGPEETARRVGSGAVFARVQPEQKVAI
                     VEALRGAGEVVAMTGDGVNDAPALRRAHIGVAMGRRGTQAARDAADLVLLEDRFPALV
                     EAVREGRRVFDNIRSSFLYLIAFKAPVFILAFLCPLFGFPLLLLPVHLVWFEMIVHPV
                     SALVFEGQEAAPGILSRPPRPARDPLLPPAQVIRALLSGLFLALLVLAIYLKHLDQGV
                     LYARSTALATQVLGSLLLLWAGRAEEGRGWRALFPAQVRFWAVVGAAALSLPLIFQVR
                     GMAALFQVQPIQFHDWALAMGLALLAVGWRFSGLFFPVGPGKGPGKP"
BASE COUNT          348 a          779 c          677 g          235 t
ORIGIN      
        1 gcaatcttga tgccaagatg ggatcacggt ttccctggac ctttccccgg ccctacgggg
       61 aagaaaaggc cgctgaaacg ccacccaacg gcgagcaggg ccagccccat cgccaaggcc
      121 caatcgtgga actggatggg ctgcacctgg aaaagggccg ccatcccccg cacctggaag
      181 atcaagggca ggctcaaggc ggcggccccc accaccgccc aaaaacggac ctgggcgggg
      241 aaaagcgccc gccaaccgcg tccttcctcg gccctacccg cccacaggag caacaggctg
      301 cccagcacct gggtggccaa ggccgtgctg cgggcgtaca gcaccccctg gtccaggtgt
      361 ttgaggtaga tggccaggac caacagggcc aggaaaagac cggacaggag ggcccggatc
      421 acctgcgcgg ggggcagcag gggatcccgc gccggcctcg gcggccggga caggatgccg
      481 ggcgcggcct cctgtccctc gaacaccagg gcggagaccg gatgcacgat catctcgaac
      541 cagaccaggt gcacgggcag caacagcagg gggaacccga aaagggggca aaggaaggcc
      601 aggatgaaca cgggggcctt gaaggcgatc aggtaaagga aggacgagcg gatgttgtcg
      661 aagacccgtc ggccttcccg cacggcttcc accagcgccg ggaaccggtc ctccagcagc
      721 accaggtcgg cggcgtcccg ggcggcctgg gtgccccggc gtcccatggc cacgccgatg
      781 tgggcccggc gcaaggcggg cgcgtcgttg acgccgtcgc cggtcatggc cacgacctcc
      841 cccgcgccgc gcagcgcctc cacgatggcg accttctgtt cgggttggac ccgggcgaaa
      901 accgcccccg accccacccg gcgggccgtt tcttccgggc cccattgggc caggtccgtt
      961 cccgtccagg ggcccccgga cccctcagcg atgccggccg cgtcggccac ggcccgcgcc
     1021 gtcagcgggt ggtcgcccgt gaccagcttg agcttcaccc ccgccccttg gcaggcccgc
     1081 accgctcccg gaacatccgg ccgcaggggg tcccggaagg ccaggagacc gtaaaggcgc
     1141 aaaccccttt catcccggga tcggtcgccc gtgcaatcgc ccccttcccg gcccgccacc
     1201 gccaagaccc ggccgccgcc gcccgccagg cgggcgttct cggactccgc cctttccctt
     1261 tcccccgggg ccagttcgca gaccgccagg atgccttcca aggacccctt ggccgccacg
     1321 cggacgtcct cccccccggc ccggcgccag acatgggcca tgcgtttccc caccgggtcg
     1381 agatcatagt catggaccaa ccgccatccc ccttgcaggc ggtccggatc cgtccccgac
     1441 cgccgggccg cttccaggat ggcttgttcc atcggatcgg cgggcgaggc ctcgcaagcc
     1501 cagagggccg cttccaacaa cccccgccca tcgaccccgg gggccagggg gaccgggggt
     1561 cccagcgtga agcggccctc ggtcagggtg ccggtcttgt ccaggcagat caccgtggtg
     1621 gaccccaggg tctccacggc cgccaaccgt ttcaccagca cgccccggcg ggacaggcgg
     1681 aaggccccca ggctgaggaa gacggccaac accaacgaga attcctccgg aatggccgcc
     1741 atgcccaggc tcaccgccga aaggaaggcg gccgcgggtc cggcgccggt ggccagggag
     1801 accaggaaca gggccgccac caacaggacc gcggccagga ccaggcggcg ggtcagcgcg
     1861 gcgatctttt cctggagcgg gctgggggac tccagcccct gggacgccaa gcgggcgatc
     1921 gcccccagac ggctgcgggg ccccgtgcgg gacacttggc ccaacccctg gcccgtcagg
     1981 accctggtcc ccgcgaaaaa gaggcggtcc ggcccctccg cccccccgcc ggaccgctt
//
LOCUS       DASTJJ010000364         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1152305, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000364 DASTJJ010000000
VERSION     DASTJJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152305"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VFR02_04595"
     CDS             <1..1096
                     /locus_tag="VFR02_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869755.1"
                     /translation="TTQALALYQDYLKEYPASTLTPRVYYLTARDYAQAADYSNAIAW
                     YEKLSTQFPQSPEALQALLDEAGLYRDKLKNTAKAGEVSQRAVSQYFANAQVKDDIQS
                     LVEAQYQSATAFFLKKDFKTANQLASSIFAAYPVLFVNPDARAKVEALADRTRRALAL
                     AQMDSTAVSLRSEEPFNPSYAADFAANSPAAEGKLVSPAGDEVVALKRVGRHDYLYLA
                     KYPPQNGKYVFKIIPHSSGANLPSWSPDGASLVYLRAVGEGRRLERVDLKQGEPKTLF
                     HSEDDSLGLYPVFHPSGTKIAFVYGGNVWVVNGDGTDKTLLKTNEKLDYTAHLSWSFD
                     GTLLRCREDGKKGKSVDEVLVLDAVGPFNP"
     gene            1113..1472
                     /locus_tag="VFR02_04600"
     CDS             1113..1472
                     /locus_tag="VFR02_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869756.1"
                     /translation="MKKFLLILPLLLAPAFARAQDATVSALQDAVKANPNSYDAHFNL
                     GVAFFNGRQYDLAAPEFKRAMDLKGGDSQAREMYESSLGIAAYLKQDFSNAIPHLKAV
                     LAVNPKNPNANLLLGNA"
BASE COUNT          294 a          501 c          460 g          251 t
ORIGIN      
        1 gaccacccag gccctggcgc tctaccagga ctacctgaag gaatacccgg cgtccaccct
       61 gaccccccgg gtctattacc tgacggcgcg ggactacgcc caagcggcgg attattccaa
      121 cgccatcgcc tggtatgaga agctgtccac ccagttcccc cagagcccgg aggccttgca
      181 ggccctcctg gacgaggccg gattgtaccg ggacaaactg aagaacaccg ccaaggccgg
      241 cgaggtttcc caacgcgcgg tgtcgcagta tttcgccaat gcccaggtca aggacgacat
      301 ccaatccctg gtggaggcgc aataccagag cgcgacggct ttcttcctga aaaaggattt
      361 caagaccgcc aaccaactgg cctcctccat cttcgcggcc tatccggtgt tgttcgtcaa
      421 tccggacgcg cgggccaagg tcgaggccct ggccgaccgc acccggcggg ccctggccct
      481 ggcccaaatg gattccacgg cggtctccct gcgcagcgag gagcccttca acccgagcta
      541 cgcggccgac ttcgccgcca acagccccgc ggccgagggc aagttggtct cccccgcggg
      601 ggatgaggtg gtggccttga agcgcgtggg ccggcacgat tacctctatc tggccaagta
      661 cccgccccag aacgggaaat acgtgttcaa gatcatcccc cacagctcgg gggcgaacct
      721 gcccagctgg tccccggacg gggccagcct ggtgtacctg cgggcggtgg gggaggggcg
      781 gcggttggaa cgggtggacc tgaagcaggg ggaacccaag accctcttcc attccgagga
      841 cgacagcctg gggctttatc ccgtcttcca tccctcgggc accaagatcg cctttgttta
      901 cggcggcaat gtgtgggtgg tcaacgggga cggcacggac aaaactttat tgaaaaccaa
      961 tgaaaagctt gactatactg cccacctctc ctggtccttt gacggcaccc tcttgcgttg
     1021 ccgggaggac gggaaaaagg ggaagtcggt ggatgaggtg ctggtgctgg acgcggtggg
     1081 gcctttcaat ccttgaacga ccggtgggca cgatgaagaa gttcctcctg atccttcctt
     1141 tgctcctggc gccggccttc gcccgcgccc aggacgcgac ggtctcggcc ttgcaggacg
     1201 ccgtcaaggc gaaccccaat tcctacgacg cccatttcaa cctgggcgtg gccttcttca
     1261 acggccgcca gtacgacctg gccgcccccg agttcaagcg ggccatggac ctcaagggcg
     1321 gcgacagcca ggcccgggaa atgtacgaga gcagcctggg catcgcggcc tacctgaaac
     1381 aggacttttc caacgccatc ccgcacctga aggccgtatt ggcggtcaac cccaagaacc
     1441 ccaacgccaa cctcctgttg ggcaacgcct aggtgcaggc gggcgacgag aaggacgcgg
     1501 aagcct
//
LOCUS       DASTJJ010000365         1095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_784093, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000365 DASTJJ010000000
VERSION     DASTJJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784093"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..397)
                     /locus_tag="VFR02_04605"
     CDS             complement(95..397)
                     /locus_tag="VFR02_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869757.1"
                     /translation="MPITEEEKQYIIEEETLRFETKKKLMQEEFGRWGGWGMGRMGWP
                     GHGCHRHFWGFGFLKVLVLILAVVGLAHLLHGRPCGWGPYYYGAPAPQSAPPGAKS"
     gene            552..>1095
                     /locus_tag="VFR02_04610"
     CDS             552..>1095
                     /locus_tag="VFR02_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9869758.1"
                     /translation="MTPKKRILVIDDDVKLGELLKGYLAGFDMAVTAAVRPEEGLAAL
                     NRLNPSLVILDVMLPGRDGFSVCREIRKGSRVPIIMLTARGDVTDRVVGLELGADDYL
                     PKPFEPRELVARIESVLRRLAPAPSGAGLLKSDALTLDLGRRQAFLKGKDLGLTPTEF
                     ETLAFFMKNPGTVLNRDQIMG"
BASE COUNT          177 a          390 c          343 g          185 t
ORIGIN      
        1 cggggcacaa aaaaaccccg gctgtagggg accggggttt tcttgaagac ccgggggccc
       61 gaaagcgccc ccggaacggc gtatcgccgg ggcctcagct cttggcgccg ggcggggccg
      121 actggggcgc cggagcccca taataatagg gcccccaacc acagggacga ccgtggagca
      181 gatgcgccaa ccccaccacc gccaggatga gcaccagcac cttcaggaat ccaaaacccc
      241 aaaagtgccg gtggcagccg tggcccggcc aacccatccg gcccatgccc caaccgcccc
      301 agcggccgaa ttcctcctgc atgagcttct tcttggtctc gaagcgcagg gtctcctcct
      361 cgatgatgta ctgcttttcc tcttcggtga tcggcatgtg cgtctcctcg cttgttttgc
      421 gccgggggtt tcgcttcccc acggccccac cctagggccc gcggggcggg aatgcttttc
      481 gcccgggttg cgctttgtca cagtttgtaa cgccccctgg ggccgtcggg ccggcgtgtt
      541 aaaatgccgc catgaccccg aagaaacgga tcctcgtcat cgacgacgac gtcaagctgg
      601 gtgagctttt gaagggctac ctggcgggct tcgacatggc cgtgaccgcc gccgtccgcc
      661 ccgaggaggg cctggccgcc ctgaaccggc tgaaccccag cctggtcatc ctggacgtca
      721 tgctgcccgg ccgggacggt ttctcggtgt gccgggagat ccgcaagggc tcccgggtgc
      781 ccatcatcat gctcaccgcc cggggcgacg tgaccgaccg ggtggtgggg ctggaactgg
      841 gcgccgacga ctacctgccc aagcccttcg agccccggga attggtggct cgcattgaaa
      901 gcgtgctgcg ccgcctggcc cccgccccct cgggcgcggg gcttttgaag tccgacgccc
      961 tgaccctcga cctgggccgc cgccaggcct tcctcaaggg caaggacctg ggcttgaccc
     1021 ccaccgaatt cgagaccctg gccttcttca tgaagaaccc cggcaccgtg ctcaaccgcg
     1081 accagatcat ggggt
//
LOCUS       DASTJJ010000366         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1763579, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000366 DASTJJ010000000
VERSION     DASTJJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763579"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1639)
                     /locus_tag="VFR02_04615"
     CDS             complement(257..1639)
                     /locus_tag="VFR02_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HET9869759.1"
                     /translation="MLLTHRTTHGKGTSEMNNRHRRPPLSVTTGAKNVAAHAGARLLM
                     DLADDVGLSGALSVALAPTKARRRGHDRGQVLVDLAVGLADGATAITDVARTFGPQPE
                     LFGPIASTATTWRMLGAIDDAALERIATARAQARREAWAAGADPRFYVIDIDATLIES
                     HSEKDQAAPNYKHSFGFHPLLAYLDATGEPLAAILRPGNAGSGTGADHVEILDAALAQ
                     LPVDPRQKEMIVRADSAGCSHEFLEAASARGVFFFVGHRLDPAMVRAIGKVPESAWSC
                     AISADGTEDADWAAVAELSDYLDLSGWPEGTRALVRREEAHPGAQLSFTDVDGHRYQV
                     FLTDHDSFDLAFLEGVYRGRGRAECAIRDSKDTGLANLPSSDFGINQAWVTAVLIAGD
                     LMAWTKANLLRGELAKATPRRLRYALFHAAGQLVRSGRRTTLRIAQGWAWADELVAAW
                     GRLPSWRLTR"
BASE COUNT          287 a          574 c          640 g          279 t
ORIGIN      
        1 tgctcgtggt ccggaggtgg gaacgggccg gacggctctc cggacgtcgg gccttggctc
       61 ctaaccccaa cctttcgcta agccgaagcc gcgtacatct gacgcattga ttgaagcccc
      121 accgggcacc gacagtcgcg gaaccgatcg ttggagtctc tcgatcgtga tgtggaaacc
      181 gctcaggaga accggcgcga ggttgggacg cgctcgacca gtcccttctt ggtggtgccg
      241 gcgaagtcgg ctgacttcag cgggtgagac gccagctggg cagccggccc caggccgcca
      301 ccagctcatc ggcccaggcc cagccctggg cgatgcgcaa ggtggttctc cgaccggagc
      361 gcaccaactg gccggcggcg tggaagaggg catagcgcag ccggcgggga gtggccttgg
      421 ccagttctcc tcggagcaag ttggccttgg tccaggccat gaggtccccg gcgatgagca
      481 cggcggtgac ccacgcctgg ttgatgccga agtcgctcga gggcaggttg gccagcccgg
      541 tgtccttgga gtcccggatg gcgcactcgg cccggcctcg accccgatag accccctcca
      601 ggaaggcgag gtcaaatgaa tcgtgatcgg tgaggaagac ctggtagcgg tgtccgtcca
      661 catcggtgaa ggacagctgg gcccctgggt gggcctcctc tcggcgcacc agggcccggg
      721 tgccttccgg ccagcccgac aggtccaggt agtcggaaag ctcggccacg gcggcccagt
      781 cggcgtcctc ggtcccatcg gctgagatag cgcaggacca ggctgattcg ggcacctttc
      841 cgatggctct gaccatggcc gggtccaggc gatggcccac gaagaagaac accccgcggg
      901 ccgaggccgc ctccaggaac tcatgcgagc aaccggcgct gtcggcccgc acgatcatct
      961 ccttctgtcg cggatccacg ggcagctggg ccagggcggc gtcgaggatc tcgacgtgat
     1021 cggcgccggt gcccgaaccg gcattacccg gtcgcaggat cgccgccagg ggctctccgg
     1081 tggcgtccag gtaggccagc agaggatgaa agccgaagct gtgcttgtag ttcggcgccg
     1141 cctggtcctt ctccgaatgg gactcgatca gggtggcgtc gatgtcgatg acgtagaagc
     1201 ggggatcggc ccccgctgcc caggcctccc ggcgggcctg ggcccgggcg gtggcgatgc
     1261 gttccaaggc ggcgtcgtcg attgccccca acatcctcca ggtggtggcg gtggaggcga
     1321 tgggcccgaa cagctccggc tggggaccga aggtgcgggc cacgtcggtg atggccgtcg
     1381 ccccgtcggc cagccccacg gccagatcga ccagaacctg accacggtca tggccccgac
     1441 gccgcgcctt ggtgggggcc agggccactg agagcgcgcc gctcaggccc acgtcgtcgg
     1501 ccaggtccat gaggagacgg gcgccggcat gggcagcgac gttcttggct ccggtggtca
     1561 ccgacagagg aggccggcgg tgcctattgt tcatctcgga agtgcccttt ccgtgggtgg
     1621 tgcggtgcgt cagcaacacc agtttcacct gcgggaaggg cgttttcgcg catggtcggc
     1681 cgaatcaggc cggagttact gcaatatcag ggctaatcga acggcggatc gagcccggca
     1741 tcggcggacg actggtggag gactacggga ccgagcagcg
//
LOCUS       DASTJJ010000367         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_784308, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000367 DASTJJ010000000
VERSION     DASTJJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784308"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="metK"
                     /locus_tag="VFR02_04620"
     CDS             <1..816
                     /gene="metK"
                     /locus_tag="VFR02_04620"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET9869760.1"
                     /translation="AGDQGMMFGYASNETPELMPAPISLAHKLTRQLTVARRSGKLPY
                     LRPDGKSQVTVEYEAPGKPARIDAVVISTQHAESVSNDELHAGILKHVIQAVLPDKWL
                     DEHTKYHINPTGRFVIGGPMGDTGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSA
                     AYAARWVAKNIVAAGLADQCEVQISYAIGVVQPTSVLVNTFGTGKLPEEQITQVVRQV
                     FDLTPRGIAKSLNLRRPIYQKTAAYGHFGRKDKDFTWERTDKAAALRKAAKLK"
     gene            910..>1827
                     /locus_tag="VFR02_04625"
     CDS             910..>1827
                     /locus_tag="VFR02_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9869761.1"
                     /translation="MKHHVKDLKLAPKGKDRIEWAERSMPVLRLIKERFSKEKPLKGV
                     KMAACLHVTTETANLLITLKAGGADVYACASNPLSTQDDVAASLVKHYGIPTFSIKGE
                     DRKTYYRHLNAVLDIRPNITMDDGADLVSMLHSERSSQASEILGSTEETTTGVIRLKA
                     MEKDGVLRFPVIAINDAKTKHFFDNRYGTGQSTMDGIIRATNLLIAGSVVVVPGYGWC
                     GRGVAMRAKGLGARVIVTEVDPLKALEAVMDGYELMPIAQAAPIGDIFVTLTGDIHVI
                     RKEHFAKMKDGAVVANSGHFNVELDLEGLK"
BASE COUNT          373 a          600 c          573 g          281 t
ORIGIN      
        1 gccggcgacc agggcatgat gttcggctac gcttcgaacg aaaccccgga actgatgccg
       61 gcgcccatct cgctggctca caaactgacc cggcagttga ccgtggcgcg caggagcgga
      121 aaactcccct atctgcggcc cgacggcaaa agccaggtga cggtggagta cgaggcgccc
      181 ggcaagccag cgcgcattga tgcggtggtc atctcaacgc agcacgcgga aagcgtgagc
      241 aacgacgaac tgcacgcggg cattctgaag catgtgattc aggctgtatt gccggataag
      301 tggctggacg agcacaccaa gtatcacatc aaccccacgg ggcggttcgt gattggtggg
      361 ccgatgggcg acacggggct caccggccgc aagatcatcg tggataccta cggcggctat
      421 ggccgccacg gcggcggcgc cttctcgggc aaggacccct ccaaggtgga ccgttcggcg
      481 gcctacgcgg cccgctgggt ggccaagaac atcgtggcgg ccgggttggc cgaccagtgc
      541 gaggtgcaga tctcctacgc catcggcgtg gtgcagccca ccagcgtgct ggtcaacacc
      601 ttcggcacgg gcaaactgcc cgaggaacag atcacccaag tggtgcgcca ggtgttcgac
      661 ctgacgcccc ggggcatcgc caagtcgctc aacctgcgcc ggcccatcta ccagaagacc
      721 gccgcctacg gccatttcgg ccgcaaggac aaggacttca cctgggagag gaccgacaag
      781 gccgccgcct tgcgcaaagc cgccaaactc aagtagggct ttttgccgcg ggatccggga
      841 tcccgcctga ctctgtatca ctccgtgtaa ctctgcggtt caatttcgca tcgggagaca
      901 aataagaaaa tgaaacacca cgtgaaggat ctgaagctgg cccccaaggg caaggaccgc
      961 attgaatggg ccgagcgcag catgccggtc ctgcgcctca tcaaggagag gttctcgaag
     1021 gaaaagccgt tgaagggcgt caagatggcg gcctgcctgc atgtcaccac cgagacggcc
     1081 aacctgctca tcaccctcaa ggccgggggc gcggacgtct acgcctgcgc ttccaacccg
     1141 ctgtccaccc aggacgacgt ggcggcctcg ttggtgaagc actacggcat ccccaccttt
     1201 tccatcaagg gcgaggaccg caagacctac taccggcacc tgaacgcggt cctggacatc
     1261 cggcccaaca tcaccatgga cgacggggcc gacctggtgt ccatgctgca ttcggaacgg
     1321 tcctcccagg cctccgagat cctgggatcc accgaggaga ccaccacggg cgtcatccgc
     1381 ctgaaggcca tggagaagga cggggtgctg cgcttcccgg tcatcgccat caacgacgcc
     1441 aagaccaagc atttcttcga caaccgctac ggcacgggcc agtccaccat ggacggcatc
     1501 atccgcgcca ccaacctgct catcgcgggt tccgtggtgg tggtgccggg ctacggctgg
     1561 tgcggccgcg gcgtggccat gcgggccaag ggcctgggcg cccgggtcat cgtgaccgag
     1621 gtcgaccccc tgaaggccct ggaagcggtc atggacggct atgaattgat gcccatcgcc
     1681 caggcggccc ccatcgggga catcttcgtg accctgaccg gcgacatcca cgtcatccgc
     1741 aaggaacatt tcgccaagat gaaggacggc gccgtggtgg ccaactcggg ccacttcaac
     1801 gtggagctgg acctggaggg cctcaag
//
LOCUS       DASTJJ010000368         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_52070, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000368 DASTJJ010000000
VERSION     DASTJJ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52070"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFR02_04630"
     CDS             complement(<1..375)
                     /locus_tag="VFR02_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET9869762.1"
                     /translation="MPTPPPPKEIKVHYRKRTALWMTAGVVFALWVFCLFNTSLFQQF
                     ESKTIDFRFGVRGARAPQAPVRIIAIDEKSLKEVGQWPWPRAVHAQLVRQLKADGVKC
                     VFYDVFFPEPERTQQQMLDQLQS"
     gene            complement(442..1242)
                     /locus_tag="VFR02_04635"
     CDS             complement(442..1242)
                     /locus_tag="VFR02_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9869763.1"
                     /translation="MALGLGRYMRLTGLFMKNCLVREMQFKANFLVRLCTEALWLVMQ
                     FIYIGVLYGQTGQIVGWTKWDMVVLVGTNHVITQLFEALFYDNCTKLIDQIRQGDLDF
                     NLIKPINTQFLVSLRYTDYASILNSSLGVAVILYGLHRMGGAVTLSGALLFAGLVVNG
                     ILIYYAILFTLSVWTFWIGRSNNLLELYWQFGQFSRYPGDIYPWLLRRLLMTVIPMLV
                     VSNFPAAVLVHKLAGGVALYGFALGLGFLAFTVWIWHRGLKRYRSASS"
BASE COUNT          315 a          445 c          483 g          267 t
ORIGIN      
        1 ggactgcagt tggtccagca tctgctgctg ggtgcgttcg ggttcgggga aaaagacgtc
       61 gtagaagacg cacttgacgc cgtcggcctt caactggcgc accagttggg cgtggacggc
      121 ccggggccag ggccattggc ccacttcctt caggctcttc tcgtcgatgg cgatgatgcg
      181 cacgggggct tggggcgccc gcgcgccgcg cacgccgaag cggaagtcga tggtcttgct
      241 ctcgaactgt tggaagaggc tggtgttgaa caggcagaag acccacagcg cgaacaccac
      301 gcccgcggtc atccagagcg cggtgcgctt gcggtaatgg accttgatct ctttgggcgg
      361 cgggggggtc ggcatggccg tcattttagg gggctttggc caaaatgcaa ataaagaact
      421 taaccaggaa aggccggcgg cctaggagga ggcgctccgg tagcgcttga ggcctcggtg
      481 ccagatccac accgtgaagg cgaggaagcc caggcccagg gcgaacccgt agagcgccac
      541 cccgcccgcc aacttatgca ccagcacggc cgccgggaag ttggacacca ccagcatggg
      601 gatgacggtc atcagcaggc ggcgcagcag ccaggggtag atgtcgcccg ggtagcggga
      661 gaactggccg aactgccagt agagttccag caggttgttg gagcggccga tccagaaggt
      721 ccagaccgac agggtgaaga ggatggcgta gtagatcagg atgccgttca ccaccaaccc
      781 ggcgaagagc agcgcgcccg aaagcgtgac ggccccgccc atgcggtgca ggccgtagag
      841 gatgaccgcc acccccaggc tcgagttgag gatggaagcg tagtcggtgt agcgcaggga
      901 caccaggaac tgggtgttga tgggcttgat caggttgaag tccaggtcgc cctggcggat
      961 ctggtcgatc aacttggtgc agttgtcgta gaagagcgcc tcgaagagct gggtgatgac
     1021 gtggttggtg cccaccagca ccaccatgtc ccacttggtc caacccacga tctggccggt
     1081 ctggccgtag agcacgccga tgtagatgaa ctgcatgacc agccacaggg cctccgtgca
     1141 gagccgcacc aggaagttgg ccttgaactg catctcgcgc accaggcagt tcttcatgaa
     1201 caggcccgtc aaccgcatgt aacgccccaa tcccaaagcc atgtccccgc ctttcgatcc
     1261 accccggggc ggatccatcc gactcaaact atagtctcca acaggaaact gttccaccac
     1321 cacgcccaag ggcggcccca ttaccaataa ataaatgttg gggcaggcac caagttcacc
     1381 aagaaaggcg atcaaattct tccgggtttt cctacttttc agcctttgca gtccttggcg
     1441 cccttgatgt cttccccacc attgaatttt tggagcgggg ccgccctccg gcgtgtgtgg
     1501 tgaaataggt
//
LOCUS       DASTJJ010000369         1120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1152793, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000369 DASTJJ010000000
VERSION     DASTJJ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152793"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFR02_04640"
     CDS             complement(<1..982)
                     /locus_tag="VFR02_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9869764.1"
                     /translation="MTPTGTQSNPEAAAAPSPFYELLQQIPLQCHETEKLMQSEILSV
                     QIDPLRQLVWMKLQVPAPVSPDCYAGLAAGLKATLPGVSKVILDVHYPAVPLADYLAL
                     HAADLVAALIEEGDIAEGWFAHCRLELSGGALVLQVPHELARQQMERKLVPKFLSEMA
                     RARCGADTAVKVEVVESLRPEITPEMLAPPPEKPKEAAKLTEPVPLLGRPIAAEPQSM
                     AVTDEQNNFVAEGEVILYEAFTTRAGRHLVKMTLTDYVDSLEVSFFHENDGKFKGGIK
                     ERDWVRVRGDLRFNKYAGDDLTLTARDIMKIPPPESRMDRAAEKRVELHCH"
BASE COUNT          185 a          341 c          394 g          200 t
ORIGIN      
        1 tgtggcagtg cagctccacg cgcttctcgg cggcccggtc catccggctc tcgggcgggg
       61 ggatcttcat gatgtcccgg gcggtcaggg tcaggtcgtc gcccgcgtac ttgttgaagc
      121 gcaggtcgcc ccgcacccgc acccaatccc gttccttgat cccgcccttg aacttgccgt
      181 cgttctcgtg gaagaagctc acttccaggg aatccacgta gtcggtcagg gtcatcttga
      241 ccaagtggcg gccggcgcgg gtggtgaagg cctcatagag gatgacctcg ccctcggcca
      301 cgaagttgtt ctgctcgtcg gtcaccgcca tggactgggg ctcggcggcg atggggcggc
      361 ccagcagtgg gaccggctcg gtgagcttgg cggcttcctt gggcttctcg gggggcgggg
      421 ccagcatctc gggggtgatc tcgggccgca gcgattccac cacctccacc ttcacggccg
      481 tgtccgcgcc gcaacgggcg cgcgccatct cggagaggaa cttggggacc agcttgcgct
      541 ccatctgttg ccgggccagc tcgtggggca cctgcagcac cagggcgccg cccgaaagct
      601 ccagccggca atgggcgaac cagccctcgg cgatgtcgcc ttcctcgatg agggccgcca
      661 ccaggtcggc ggcgtggagc gccaggtaat cggccagggg cacggcgggg taatgcacgt
      721 ccaggatgac cttggacacg cccggcaggg tggccttgag cccggcggcc aggcccgcgt
      781 agcagtcggg cgagaccggg gcgggcacct gcagcttcat ccagaccaac tggcgcaggg
      841 ggtcgatctg cacgctcagg atctcggatt gcatgagttt ttcggtctcg tggcactgca
      901 gggggatctg ctggagcagc tcgtaaaagg gcgaaggggc ggcggcggct tcggggttgg
      961 actgtgtgcc ggtcggcgtc atacggcctc atccgcggga atttgggagg gttggaggtt
     1021 gtatccgaac tatacaacct tgaggggaaa aatcaagaat tttgggacgg ttcgacgggc
     1081 aaaaccaaaa ataaatcccg acgcctacgg atcgaaataa
//
LOCUS       DASTJJ010000370         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_908506, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000370 DASTJJ010000000
VERSION     DASTJJ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908506"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1161
                     /locus_tag="VFR02_04645"
     CDS             187..1161
                     /locus_tag="VFR02_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET9869765.1"
                     /translation="MHSIVTGGAGFVGSHLCDHLLAKGHRVTCVDNLITGRKENIAHL
                     RRHPGFRFIRRDVTRGLPRISRDMAKAFPRFLPRADYVFHLASPASPVGYMKNSIETI
                     LTNSMGSLRLLEYCRKRKAKFLFASTSEVYGNPAVHPQAETYWGNVNSFGPRSCYDES
                     KRLGEALVYEYIHKYGVDARIIRIFNTYGPRLNENDGRVVSNLISQALRGQALTLYGD
                     GSQTRSFCYVADLVAGIWAAISKEDTRGQIFNLGNPQETTIKDFAKVVLELCGAPGAK
                     LVHEALPQDDPVRRKPDISKAKKVLGWEPHISLKEGLRQTIDYYRGRI"
BASE COUNT          229 a          464 c          354 g          223 t
ORIGIN      
        1 gcccaccgcc cgccgcgccc ccgggccctt ggatatgccc gtgcccacgc ccaccccggc
       61 ccaggcggac ccctacagcg gcgggggcta tcccatccgt tgacgcccgg catggcgccc
      121 cgggacaccc tgtgttagac tccctttccg gatatggcct ctcccattcg actccaacga
      181 gggtttatgc attccatcgt gaccggcggg gcgggatttg tcgggtccca cctctgtgac
      241 cacctgctgg ccaagggcca ccgggtgacc tgcgtggaca atttgatcac cggccgcaag
      301 gagaacatcg cccacctgcg gcggcatccc ggcttccggt tcatccgccg ggacgtgacc
      361 cgggggctcc cgcgcatctc gcgggacatg gccaaggcct tcccccgttt cctgccccgg
      421 gccgattacg tcttccacct ggcgtccccc gcctccccgg tgggttatat gaagaactcc
      481 atcgagacca tcctgaccaa ctccatgggc agcctccggc tcttggaata ttgccgcaag
      541 cgcaaggcca aattcctctt cgcctccacg tcggaggtct acggcaaccc cgcggtccat
      601 ccccaggcgg agacctactg gggcaacgtg aactccttcg gtccccggtc ctgctacgac
      661 gagagcaagc gcctgggcga ggccctggtc tacgaataca tccacaagta cggcgtcgac
      721 gcccggatca tccgcatctt caacacctac ggtccccgcc tgaacgagaa cgacggccgg
      781 gtggtgtcca acctgatctc ccaggccctg cgcggccagg ccctgaccct ttacggcgac
      841 ggctcccaga cccgcagctt ctgttacgtg gccgacctgg tggccggcat ctgggccgcc
      901 atctcgaagg aggacacccg ggggcagatc ttcaacctgg gcaaccccca ggaaacgacc
      961 atcaaggact tcgccaaggt ggtcctggaa ctctgcggcg cccccggcgc caagctggtc
     1021 cacgaggccc tgccccagga cgatccggtg cggcgcaaac ccgacatttc caaggccaaa
     1081 aaagtcctcg gctgggaacc ccatatttcc ttaaaagagg gcctaaggca gacaattgat
     1141 tattaccggg gtagaatatg agccttgggc gtctgtaccc tttgaccgaa cgcccgcccc
     1201 gcgttcccca tcggcccgtg aaatcgtttt aaagaggtgc tatgaaagcc gcgatccttt
     1261 acccgccgtt
//
LOCUS       DASTJJ010000371         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1642264, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000371 DASTJJ010000000
VERSION     DASTJJ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642264"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..700
                     /locus_tag="VFR02_04650"
     CDS             119..700
                     /locus_tag="VFR02_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869766.1"
                     /translation="MKACYLGVLFALWLAAAPLFAAATAPVKAKNLAPPAIDPVVQRQ
                     PILAKWTTLEGMRYYYDGVLLDNGKGLGLAQVLYPLNDGQATGLLRKSEDDESLGLGA
                     VLGGSLLMGAGLLTSDYDQANRQVHVPVGSAIALIVGIVADFAGGLWLEESRTAKFAA
                     VERYNQVVRGEAGPSVENWKAIPAADLSVAFAF"
     gene            789..>1283
                     /locus_tag="VFR02_04655"
     CDS             789..>1283
                     /locus_tag="VFR02_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869767.1"
                     /translation="MRRILALLSLFFALATGAGWAQTATYTPTLTSTPTRTVTSTPTL
                     TPSSCPTTTPIGTPTQTPTATRIYTPTLTATPTVTGGAVTYTSTVTPTPTVTGGTPAW
                     TRTPTYTPTVTDTRTPTPGSPTVTPTWTLSPTLTPTPAPGQPMTYSVNDETTVAQAMG
                     CDTQT"
BASE COUNT          222 a          470 c          373 g          218 t
ORIGIN      
        1 ctgcgggttg gacccggcag ccccccgaaa gttcccgcgc ccggggattt tgatgggccc
       61 catggtcgaa agcccctaaa atgcccggga tccactattt caaaaaagga gatccaatat
      121 gaaagcatgc tatttaggcg tgttattcgc gttgtggctg gcggcggccc cccttttcgc
      181 ggcggctacg gccccggtca aggccaagaa tttggcgccc ccggcgatcg acccggtggt
      241 ccagcgccag cccatcctgg ccaagtggac caccctggaa ggcatgcgct attactatga
      301 cggggtcctt ttggacaacg gcaaggggct gggcctggcc caggtcctct atcccctgaa
      361 cgacgggcag gccaccggcc tgttgcgcaa gtccgaggat gatgagtccc tcggcttggg
      421 cgcggtcctg ggcggaagcc tgttgatggg cgcgggcctg ttgacctcgg attacgacca
      481 agccaaccgg caagtgcatg tgcccgtggg ctcggccatc gccttgatcg tcggcatcgt
      541 ggccgacttc gccggggggc tttggctgga agaatcccga actgccaagt tcgccgccgt
      601 ggagcgctac aaccaggtgg tccgcgggga agcgggacct tcggtggaga actggaaggc
      661 catcccggcc gcggacctgt cggtggcctt cgcgttttga cccaggacgg ctgatttttt
      721 ggaataaaaa ggcccgggtt cccactggta aggaagccgg gtttttttat tccatcctgg
      781 ggtacgccat gcgccgcatt ttggcccttt tatccttatt tttcgccctt gcgacggggg
      841 cgggctgggc ccaaaccgcc acctataccc ccaccctgac ctcgaccccg acccggaccg
      901 tcacctccac gcccaccctg accccctcgt cctgtcccac gaccacgccg atcgggacgc
      961 cgacccaaac cccgaccgcg acccggatct atacgcccac cctgaccgcc acccccacgg
     1021 tgacgggcgg ggccgtgacc tatacctcca ccgtgacgcc cacccccacg gtgaccggcg
     1081 ggaccccggc ctggacccgc accccgacct atacgccgac cgtgaccgac acccggaccc
     1141 ccacgcccgg gtcccccacc gtcaccccca cttggacctt gtcccccacc ctgacgccca
     1201 ccccggcgcc gggccaaccc atgacctact ccgtcaacga cgaaacgacc gtggcccagg
     1261 cgatgggctg cgacacccaa acg
//
LOCUS       DASTJJ010000372         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1031449, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000372 DASTJJ010000000
VERSION     DASTJJ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031449"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFR02_04660"
     CDS             complement(<1..407)
                     /locus_tag="VFR02_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869768.1"
                     /translation="MRPFLAAAALAAFLLCSAVPLSSGRDTLAARQFETAKRCFERQL
                     YFAAQRWAHLASLNAPRAAGPWVLTGHCFYLRAQDAMAAYCYARARALDPDLEHLPLF
                     TAKDHPAAAGPRPALSGAALRGLQRKIGQMIMV"
     gene            577..1035
                     /locus_tag="VFR02_04665"
     CDS             577..1035
                     /locus_tag="VFR02_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET9869769.1"
                     /translation="MLLAAGLFSGCAAIRQSMDRGNLFGEAPGSFNAAPGAVPAGAPL
                     DDAWKSQMEGVASWYGPEFNGRLTASGEVYDMYAYTAAHRTLPLGTVVRVTDEENGKT
                     VEVRVNDRGPFKKDRIIDLSKSACQALDMMGNGTARVKLDVIRWPEKAGG"
     gene            1038..1865
                     /gene="pyrF"
                     /locus_tag="VFR02_04670"
     CDS             1038..1865
                     /gene="pyrF"
                     /locus_tag="VFR02_04670"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9869770.1"
                     /translation="MAPLEPFSKRLQAAVSRTGGCLCVGLDVDLAKMPKDIPADGPGA
                     LRFCRAIIEATRPYAAAYKANLAFFESLGTRGFEVLELLRRSIPPDALFIADAKRGDI
                     GNTSQAYAQALYGNLRSDSVTLSPYMGKDSVEPFLKYPGTCSFVLCLTSNPSAADFQL
                     QALEGGEKLYERVARTAPGWAKGQSGEVGLVVGATKAEYVKSLRVLAPEAAFLVPGVG
                     AQGGDLKAVLEAGRAKDGAGILVNASRSILYASAGPDYAEAAAAEAAKMVGQMKAYF"
     gene            1897..>2170
                     /locus_tag="VFR02_04675"
     CDS             1897..>2170
                     /locus_tag="VFR02_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9869771.1"
                     /translation="MTNDEVLKHFEETHALLKGHFRLSSGRHSDRYLQCALVLQYPWI
                     AEKLCEALAEKVRPLEPQAVVSPALGGLVVGQELARALKVRAIFTER"
BASE COUNT          369 a          703 c          757 g          341 t
ORIGIN      
        1 ctcaccatga tcatctggcc gatcttgcgc tgcagcccgc gcagggccgc gcccgagagg
       61 gccgggcggg ggccggcggc ggccgggtgg tccttggccg tgaacagggg caggtgctcc
      121 aggtccgggt ccagggcccg ggcccgcgcg tagcaatagg ccgccatggc gtcctgggcg
      181 cgcaggtaga aacaatggcc ggtcagcacc caaggccccg ccgcccgcgg cgcgttgagg
      241 gaggccaaat gggcccaacg ttgggcggcg aagtaaagct ggcgctcgaa gcagcgcttg
      301 gcggtctcga actgccgggc ggccaatgtg tcccggccgc tggaaagcgg gacggcggag
      361 cagagcagga aagcggcgag ggcggccgcg gcgaggaagg ggcgcatggg tcgattttaa
      421 caaagaaggg ccccggcggg gagcggacag catgaactat cctgttgatt cgggtggggg
      481 acgccaatta tcctgaatcc cgcacccact ccctgacaaa ggaaaccagc catccgcctc
      541 caccgccccc tttcgagcct gacgcgcccc ctcctgttgc tgttggcggc gggcctgttc
      601 tcgggatgcg cggccatccg ccaatccatg gaccggggca atttgttcgg ggaggccccg
      661 ggatccttca acgccgcgcc gggggcggtg ccggccggcg cccccttgga cgacgcctgg
      721 aaaagccaga tggagggggt agcctcctgg tacgggcccg agttcaacgg ccgcctgacg
      781 gccagcgggg aagtgtacga catgtacgcc tatacggcgg cccaccgcac cctgcccctg
      841 ggcacggtgg tgcgggtcac cgatgaggag aacggcaaga ccgtggaggt ccgggtgaac
      901 gaccggggcc ccttcaagaa ggatcggatc atcgaccttt ccaagtcggc ctgccaggcg
      961 ctggacatga tgggcaacgg gaccgcccgg gtgaagctgg acgtgatccg gtggcccgaa
     1021 aaggctggcg gttgaggatg gcccccctgg aacccttttc caaacggctc caagccgccg
     1081 tctcccgcac cgggggctgc ctttgcgtgg gattggacgt ggacctggcc aaaatgccca
     1141 aggatatccc ggcggacggc ccgggtgccc tgcgcttttg ccgcgccatc atcgaggcca
     1201 cccggcccta cgccgccgcc tacaaggcga acctggcctt cttcgagtcc ctggggaccc
     1261 gtgggttcga ggtgctggag ctcttgcgcc ggtccatccc cccggatgcg ctcttcatcg
     1321 ccgacgccaa gcggggcgac atcggcaaca cctcccaggc ctacgcccag gccctttatg
     1381 ggaacctccg gtccgattcg gtgacccttt ccccctatat ggggaaggat tcggtggaac
     1441 ctttcctgaa atatcccggc acctgttcct tcgtgctctg cctgacctcc aacccttcgg
     1501 cggcggactt ccaattgcag gcgctcgagg ggggcgaaaa actgtacgag cgggtggccc
     1561 ggaccgcgcc cggttgggcc aagggccaaa gcggggaggt ggggttggtg gtgggggcca
     1621 cgaaggctga atatgtgaaa tccctgcggg ttttggcgcc cgaggccgcc ttcctggtgc
     1681 ccggcgtggg ggcccagggc ggcgacctga aggcggtcct ggaagcgggg cgggccaagg
     1741 atggggcggg gatcctggtg aatgcctcgc ggtccatcct ctacgcttcc gccgggccgg
     1801 actatgccga agccgcggcg gccgaagccg ccaaaatggt cggtcagatg aaggcatatt
     1861 tttaacgtaa agagatcggc tttcccggag acccagatga ccaacgacga agtcctgaaa
     1921 catttcgaag agacccacgc cctcctgaag ggccacttcc gcctttcctc gggccgccac
     1981 agcgaccgct accttcaatg cgccctggtc ctgcaatatc cctggatcgc cgagaagctc
     2041 tgcgaggcct tggccgagaa ggtgaggccc ctggaacccc aagccgtggt gtccccggcg
     2101 ctgggggggc tcgtggtggg ccaggaactg gcccgggcct tgaaggtgcg ggccatcttc
     2161 accgaacgca
//
LOCUS       DASTJJ010000373         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_909168, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000373 DASTJJ010000000
VERSION     DASTJJ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909168"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /gene="cas1"
                     /locus_tag="VFR02_04680"
     CDS             complement(<1..1530)
                     /gene="cas1"
                     /locus_tag="VFR02_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HET9869772.1"
                     /translation="MEVEGIFAPSADTEKGKAVHKRVDRPSLEADGDDENESAEPDKP
                     KSVRSLALTSQSLGLTATLDLAEITGDKAVPIEYRKGRPRHSPTNAEEEDPDADEPSL
                     PVAEPWPTDCVQVGLQSLLLEEAGYRVEEAVLYYAEEKRRLTIGVDQELKNQALETLQ
                     AAKTCAKGPRPLPLVNDPKCPGCSLVTICLPDEVRFERGGGGDEAGPRRLWPPRDDGI
                     QVVAQTEGSRLGVSGFSLKVTGKKGEKLKEVPLANVESLALLGHVQVTSQALRVLADR
                     GIPVAFLSAAGRLTAMVDPLDSVSARIRRSQVLRCEDPAAVLELSRALVAAKIANQRT
                     LLMRNHAGFPKEEAAEMVESLEKAGKAATLEELRGHEGQAAAVYFRNFAGMLKTPLAG
                     EFQENGRQRRPPPDPVNACLSFGYAMLAHECTAALRLARLEPSIGCFHVSRPGRPALS
                     LDLMEPFRPLIADSVAVSAFNRGELGEGHFLRTSAGCALTDSGRKAFFNAYGRRMAAD
                     VT"
BASE COUNT          254 a          527 c          490 g          278 t
ORIGIN      
        1 ggtcacgtcc gccgccatcc gccgaccgta agcgttgaag aaggccttcc tgccggaatc
       61 cgtcagggcg catcccgcgc tggtgcgtag aaaatgcccc tcccccagtt cgccccggtt
      121 gaaggccgaa accgccacgg aatcggcgat gagcggccgg aaaggttcca tcaggtccag
      181 cgaaagggcc gggcgtcccg ggcgtgaaac gtggaaacag ccgatggaag gctccaaccg
      241 cgccagccgc agggccgccg tgcattcatg cgccagcatg gcgtagccga aggagaggca
      301 ggcgttcacc gggtccggcg gcggccgccg ctgacgcccg ttttcctgga attcccccgc
      361 caggggcgtt ttcaacatgc ccgcgaagtt gcggaaatag accgccgcgg cttggccttc
      421 gtggccccgc aactcctcca aagtcgcggc ctttcccgcc ttttccagcg attccaccat
      481 ctccgccgcc tcttccttcg ggaatccggc gtggttgcgc atcaataaag tgcgctggtt
      541 ggcgatcttg gcggccacca gggcacggga taattccagg accgccgccg gatcctcgca
      601 ccgaaggacc tgcgagcgcc ggatgcgggc gctcaccgaa tccaaggggt ccaccatggc
      661 cgtcaagcgg cccgccgcgg acaggaaagc caccggaatg ccgcggtccg ccagcacccg
      721 cagggcctgg ctggtgacct ggacatggcc caatagggcg aggctttcca cgttggccag
      781 cgggacctcc ttgagtttct cccccttctt gccggtgacc ttgagggaaa acccgctgac
      841 gcccaggcgg ctgccctcgg tctgggccac cacctggatg ccgtcgtccc gtggcggcca
      901 aaggcgccgg ggcccggctt cgtccccccc gccgccccgt tcaaagcgga cctcatccgg
      961 caggcagatg gtgacgagcg agcaaccagg gcatttcggg tcattgacca ggggaagcgg
     1021 gcggggcccc ttggcgcatg ttttggccgc ctggagggtt tcaagcgcct ggtttttcaa
     1081 ctcctggtcc acgccgatgg tcaagcggcg cttttcctcg gcgtaataaa ggacggcctc
     1141 ctcgacccgg taacccgcct cctccagcag gagggactgg aggcccacct ggacgcagtc
     1201 cgtgggccag ggctccgcca cgggaagcga gggttcatcg gcgtcggggt cttcttcctc
     1261 ggcgttcgtt ggggaatgcc tcggtcgtcc cttgcggtat tcaatgggca ctgccttgtc
     1321 gcccgtgatc tccgcgaggt ccaacgtcgc cgtcagtccg aggctttggc tggtcagggc
     1381 caggctacgg acggatttcg gcttatccgg ttccgccgac tcattttcgt cgtccccatc
     1441 ggcctcgagg ctgggccggt ccacccgttt atggacggct ttcccctttt cagtatccgc
     1501 gctgggggca aaaatacctt ccacttccat gaagtaaaac aaacgagga
//
LOCUS       DASTJJ010000374         1141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_909555, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000374 DASTJJ010000000
VERSION     DASTJJ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909555"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFR02_04685"
     CDS             <1..515
                     /locus_tag="VFR02_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869773.1"
                     /translation="GASYAAIKVPKNSVGTKQLKANAVTGAKVKNGSLTTGDFGQKLP
                     AGPVGEEGPPGPPGPPGAPGVASVATRWGPEDSISGDTPEIAVAACAPGELVTGGGID
                     TFGLSAKHEYVLMDSRPAVKEEVVEFEQNFVRHPAPADGAQATEWLIRVREEGPNTTK
                     YRPYVQCASP"
     gene            complement(753..>1141)
                     /locus_tag="VFR02_04690"
     CDS             complement(753..>1141)
                     /locus_tag="VFR02_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869774.1"
                     /translation="VARKGTVQALGRTVLTTRGGHTLYSLSVERHGRFICKGSCLLTW
                     HPLLIARGVKPKGPVKLRTIRRPEGRRQVTFRGRPLYSFSGDLKKGEANGEGFKDVGT
                     WHAAAVGPIGETQPQPPPESPYPYPY"
BASE COUNT          208 a          357 c          395 g          181 t
ORIGIN      
        1 gaggagcctc ctacgcggcg atcaaggtcc cgaagaacag cgttggtaca aaacagctca
       61 aggccaatgc cgtaacggga gccaaggtca agaatggctc gttgacgacc ggagacttcg
      121 ggcagaaact tcccgccggc cccgtgggag aggaagggcc gcctgggccg cctggacccc
      181 ccggagcgcc gggtgtggct agcgtcgcca cgcgttgggg tcctgaagac tccatcagcg
      241 gtgacacccc cgagatcgcc gtcgccgcct gtgcgccggg ggagcttgtc acggggggcg
      301 ggatcgacac gtttggcctg agtgcaaagc atgaatacgt cctcatggat agccggcccg
      361 ccgtaaaaga agaagtcgtg gaattcgaac aaaatttcgt ccgccaccct gcgccagcag
      421 atggagcgca ggccaccgaa tggttgatcc gcgtccgaga agaaggacct aacacaacga
      481 aataccgtcc ctacgttcaa tgcgcttcac cctgaacgcg gccgcctcta gcctgaagag
      541 ggggtcgctt agagctggca atcggccgac ctcgaaagcg gatgggaaaa cgtgccaggg
      601 gcgtttgaga gcgtcggctt ctgcaaggac cagacgggag tcgtccacct caagggagtg
      661 gccaacctcg gtccggccgg agctacccag tcagctctgg gacgaccatc gggctcgacg
      721 ggatcagctt ccgcgccgag tcctgaggtc gctcagtagg ggtacgggta cggcgattcg
      781 ggtgggggtt ggggctgcgt ttcgccgatc ggacccacgg cggccgcgtg ccaggtgccg
      841 acgtccttga agccctcgcc gttcgcttcg cccttcttca ggtctccgga gaagctgtag
      901 agggggcggc ccctgaaggt gacctgcctg cgtccctccg ggcggcggat ggtgcgcagc
      961 ttgaccgggc ccttgggctt cacgcccctc gcgatcagga gcgggtgcca ggtgaggagg
     1021 cacgagccct tgcagatgaa gcggccgtgg cgctcgacgc tgaggctgta gagcgtgtgc
     1081 cccccgcggg tggtcagcac ggtccgcccc agggcctgga cggtcccctt ccgggccacc
     1141 c
//
LOCUS       DASTJJ010000375         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1643237, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000375 DASTJJ010000000
VERSION     DASTJJ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643237"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFR02_04695"
     CDS             complement(<1..1064)
                     /locus_tag="VFR02_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9869775.1"
                     /translation="MLELQALQVLSTVNCGWLWMIPAAPLAGALANGVLSWRRPAHPH
                     RLASGIALTAAGLSFFLAFLAFLTFKGLPDNAVLAQGLLDWMEAPHLRFDAGLQLDDL
                     SLVMVLFITWVAGWIHLYAAGSPAPGGGGARYLVALNLMLFFLLVLVMADGLFLMFLG
                     WEGVGLCAVYLIGAGSTEGPGAQAAQKALAIHRLSDGAFLAGLLLVTILAGTGDFNSL
                     QELRQDFRGAAGLVCACFLVAALGKAAQFPLSTWLPGSFVGPVPGLALLYAVSLGATG
                     VYLVARLHFLFVLAPWITTAMALAGAVTALGAAFLSASQTQLKKILSYWTLSQVGLMF
                     LALGVSAYTAGLFHLLSFGF"
     gene            complement(1278..>1814)
                     /locus_tag="VFR02_04700"
     CDS             complement(1278..>1814)
                     /locus_tag="VFR02_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HET9869776.1"
                     /translation="GLALGAGRASAQTFDLGVEAGANFSSFIGQDVSSVGGLNASRMG
                     FVGGGFLALNFGPNFALRPEVLFEQKGAAVSGTSTSTELDYIEVPVLLKFGLGTPVIN
                     PNILLGPSFAWSTVSKDSNGDFSNINSSDVGLVGGVEIDVDKFLVSGRYELGLDNVQK
                     NVNVQNGTFTLLVGYSFL"
BASE COUNT          395 a          596 c          589 g          234 t
ORIGIN      
        1 aagaacccga aggacagcag gtggaacaag cccgccgtat aggcgctcac gcccagggcc
       61 aggaacatga gccccacctg ggacagcgtc caataggaaa ggatcttctt gagctgggtt
      121 tgggaagccg acaggaacgc cgcgcccaag gccgtcaccg ccccggcgag ggccatggcg
      181 gtggtgatcc agggggccaa cacgaagagg aaatgcagcc gggccaccag gtaaacgccc
      241 gtggcgccca gggacacggc gtagagcagg gccaaccccg gcacggggcc cacgaaagaa
      301 cccggcagcc aggtcgacaa ggggaactgg gcggctttcc ccagcgcggc caccaggaaa
      361 caggcgcaga ccaggccggc ggcgccccgg aagtcctggc gcagttcctg gagcgaattg
      421 aaatcacccg tgcccgccag gatggtcacc agcagcagcc ccgccaggaa agcgccgtcg
      481 ctcaagcggt ggatggcgag cgccttttgg gccgcctggg cgcccgggcc ttcggtggaa
      541 cccgccccga tgaggtacac cgcgcaaagc cccacgcctt cccaacccag gaacatgagg
      601 aacaacccgt cggccatgac caacaccagc aggaagaaca acatcaggtt caaggccacc
      661 aggtagcgcg cgccgcctcc cccgggggcc ggcgaccccg ccgcgtagag gtggatccag
      721 cccgcgaccc aggtgatgaa gagcaccatc accagggaga ggtcgtccaa ttggagcccg
      781 gcgtcgaagc gcaggtgggg ggcctccatc caatccaaca ggccctgggc cagcacggcg
      841 ttgtccggca ggcctttgaa ggtgaggaag gccaggaagg ccaaaaagaa ggaaaggcca
      901 gcggccgtca aggcgatgcc ggaggccaag cggtgcgggt gggcgggacg gcgccaggac
      961 aggaccccgt tggccaatgc ccccgccaat ggcgcggccg ggatcatcca aagccagccg
     1021 caattgacgg tggaaagcac ctgcagggct tgtagttcga gcatcccgtc tcccgaaggt
     1081 ggattggccc catcatccca caatccccgg gggccttaac agggcgccga aaattatttt
     1141 tcagcgcccc actaacggcc gtcctcccgg gggaaagtcc atccgaacgg acttttttca
     1201 acgtcatttc acgcaataaa aaagcccgat ggcgcgtgag gcgcgccgtc gggccctgcc
     1261 ggtggtcgaa ggaccggtca gaggaaggag tagcccacca gcagggtgaa ggtgccgttc
     1321 tggacgttca cgttcttttg gacgttgtcc aggcccagtt cataccgccc gctgaccagg
     1381 aacttgtcca cgtcgatctc cacgccgccc accaacccca cgtcggagga attgatgttg
     1441 ctgaaatcgc cgttggagtc cttggaaacc gtgctccagg cgaacgaggg ccccagcagg
     1501 atgttggggt tgatcacggg cgtacccagg ccgaacttga gcagcacggg cacctcgata
     1561 tagtccagct cggtggaggt gctggtgccc gaaacggcgg cccccttctg ctcaaaaagc
     1621 acctcggggc gcagggcgaa gttggggccg aaattgagcg ccaggaaccc gccgcccacg
     1681 aaccccatcc gggaggcgtt caggccgccc accgaactca cgtcctgacc gatgaagctg
     1741 gagaaattgg ccccagcctc cactcccagg tcgaaggtct gggccgaagc gcggcccgct
     1801 cccagggcca aacc
//
LOCUS       DASTJJ010000376         1457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1398726, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000376 DASTJJ010000000
VERSION     DASTJJ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398726"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="argH"
                     /locus_tag="VFR02_04705"
     CDS             complement(<1..1260)
                     /gene="argH"
                     /locus_tag="VFR02_04705"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET9869777.1"
                     /translation="MKKTWGGRFQKPTDPRVEAFTESISFDHRLGAFDLAGSRAHVRT
                     LTGAGLLTAAEGRKLLAGLAQVEKKLKAGKLPLDRSLEDIHTAVERALTQLVGPLGGK
                     LHTGRSRNDQIATDLRLYLRHATAGAVTAGLGLVDTLASLALREKATLMPGYTHLQRA
                     MPVTLGHHLAAYGFMVSRDLDRFLAAWDRADASPLGSGALAGTNHAFDRAKTAKHLGL
                     KAALPNSLDAVSDRDALLDFLAAAAVTQMHLSRLSEDLVLWASSEFNFIRMDDAFATG
                     SSLMPQKKNPDVVELIRGKSGRVFADLLGLLTVLKGLPLAYNRDLQEDKHFLFDAADT
                     VTDSLALMAAFLGSLRFNRDVMRRAAESDFGILATDLADLLVKQGVPFRQTHETVGRI
                     VQYCLAQGKGLRDLSDAELRGFSHRFPL"
BASE COUNT          236 a          462 c          512 g          247 t
ORIGIN      
        1 caaggggaag cggtgggaga aaccccgcag ctcggcgtcg gacaggtccc gcaggccctt
       61 gccctgggcg aggcaatatt ggacgatccg gcccacggtc tcatgggtct gccggaaggg
      121 cacgccctgc ttgaccaaca ggtcggccag gtcggtggcc aggatgccga aatcgctctc
      181 ggcggcccgg cgcatcacgt cccggttgaa gcgcaggctg cccaggaagg ccgccatgag
      241 cgcgagcgaa tccgtcacgg tgtcggcggc gtcgaagagg aaatgcttgt cctcctgcag
      301 gtcgcggtta taggcgaggg gcaggccctt gagcacggtc agcagcccca gcaggtcggc
      361 gaagacccgg ccggacttgc cccggatcag ttccaccacg tcggggttct tcttttgggg
      421 catcaggctg gagccggtgg cgaaggcgtc gtccatgcgg atgaagttga actcggagct
      481 ggcccaaagc accaggtcct ccgacaggcg ggacaggtgc atctgggtca cggcggcggc
      541 ggccaggaag tccagcaggg cgtcccggtc cgacaccgcg tccaggctgt tgggcagggc
      601 cgccttcagc cccaggtgct tggcggtctt ggcccggtcg aaggcgtggt tggtcccggc
      661 cagggcgccg gaccccaggg gcgaggcgtc ggcccggtcc caggcggcga ggaaccggtc
      721 caggtcccgg gagaccatga agccgtaggc cgccaggtgg tggcccaggg tgaccggcat
      781 ggcccgttgc aggtgggtgt agcccggcat cagggtggcc ttttcgcgca gcgccagcga
      841 ggccagggtg tccaccagcc ccaacccggc cgtgacggcc ccggcggtgg cgtgccgcag
      901 gtagagccgc aggtcggtgg cgatctggtc gttgcgcgag cggcccgtgt gcagcttgcc
      961 gcccaggggg cccaccagct gggtcagggc ccgctccacg gccgtatgga tgtcctccag
     1021 gctgcggtcc agcggcagct tgccggcctt gagcttcttt tcgacctggg ccagccccgc
     1081 gaggagcttg cggccctcgg cggcggtcaa gagcccggcg ccggtcaagg tgcggacatg
     1141 ggcccggctc cccgccaggt cgaaggcgcc caaccggtgg tcgaaggaga tcgactcggt
     1201 gaaggcctcc acccgcgggt ccgtgggttt ctggaaccgt ccgccccatg tttttttcat
     1261 gagcaactcc gatttcgatt ctaaaagctc ttatcgaaaa tcaaaacaat tttcaccacc
     1321 aaggcaccaa ggtcaccaag aaagacgatc taatttatcc gaagcttcca actctttatc
     1381 ttcgcagtcc ttggtgcact tggtgtctgc cccatcattg aatttttgga gcggggccgc
     1441 ccttgggcgt gtgtggt
//
LOCUS       DASTJJ010000377         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1275799, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000377 DASTJJ010000000
VERSION     DASTJJ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275799"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFR02_04710"
     CDS             complement(<1..1260)
                     /locus_tag="VFR02_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869778.1"
                     /translation="MINSFFGINTTLNGLLLQQAEQNVLASNIAKAGSYVDANGYVMT
                     TDQRLNVTQGSPFLFNTLNGPSALGTGPVLQSITRMRNSYLDRQIQLQSQTVGKEQAL
                     AATLAQIQTILNGSATTTLGYAITQLGSAFSGLATASAPVAAAASQLRADTAAGAPAA
                     TLLADQAALTAAEADPSIAAAQQAALNAGVNFATLANTQYRQLQDLQTTMNSQIGQDV
                     TDVNNLLQQINQINKEILGSAGANVNDLLDARDYDITLLSRLANVQATYASNGTATVT
                     LGGLALVNAAGAAILSTNVSNPHNPGLADITIQSPQGTYTFTDPTGLITSGKLGGDLL
                     ARDGVVQGYLEQVDQIAFSVSQLANLIYSSGYAPGSATAAGTTGINFFVGGAIQTPNT
                     RNIAQTPNPANTPVVNGSLQVGASAADI"
     gene            complement(1271..1765)
                     /locus_tag="VFR02_04715"
     CDS             complement(1271..1765)
                     /locus_tag="VFR02_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HET9869779.1"
                     /translation="MTAPLFRELAEVMAKETECYRRLSALAGEQRDILVSSKIEALPD
                     NVRSGEKQVFQLGPLAGRRNELLAEIGKTLGVKKMDLAQAIQAAPGEVSEVFRESALL
                     LAKTARELEALNQGNDKLLRNALSYVNFTLKAVRTAGRPSAARAGAEKPQTQAAPAFV
                     NRTV"
BASE COUNT          281 a          559 c          653 g          362 t
ORIGIN      
        1 gatgtcggcc gccgaggcgc ccacctgcag gctgccgttg accaccggcg tgttggccgg
       61 attgggggtc tgggcgatgt tgcgggtatt gggcgtctgg atggcgcccc ccacgaagaa
      121 gttgatcccg gtggtgccgg ccgctgtggc gctgcccggg gcgtagcccg aactgtaaat
      181 gaggttggcc aattggctca ccgagaaggc gatctggtcc acctgttcca agtatccctg
      241 gaccaccccg tcccgcgcca acaggtcgcc ccccagcttg cccgaggtga tgaggccggt
      301 cggatcggtg aaggtatagg tgccttgggg ggactggatg gtgatgtccg ccaggcccgg
      361 attatggggg ttgctcacgt tggtggaaag gatggccgcc cccgccgcat tgaccaaggc
      421 caggcctccc aaggtgaccg tggcggtccc gttggaggcg taggtggcct ggacattggc
      481 caaccgggac aggagggtga tgtcgtaatc gcgcgcgtcg agcaggtcgt tcacattggc
      541 ccccgccgaa cccaggatct ccttattgat ctggttgatc tgctggagca ggttgttgac
      601 gtcggtcacg tcctgtccga tctgggaatt catggtggtc tgcaggtcct gcaactgccg
      661 gtactgggtg ttggccaggg tggcgaaatt gaccccggcg ttcaaagcgg cttgttgggc
      721 cgccgcgatg gaagggtccg cctcggcggc ggtcaaggcc gcttggtcgg ccaagagggt
      781 ggccgcgggg gcgccggccg ccgtgtccgc ccgcaactgg gaagccgcgg ccgccaccgg
      841 ggccgaagcc gtggccagcc ccgaaaaagc gctccccaac tgggtgatgg cgtagcccag
      901 ggtggtggtg gcgctgccgt tgaggatggt ttggatctgg gccagggtgg cggccagggc
      961 ctgttccttg cccaccgtct ggctttgcag ttggatctgg cggtccaggt agctgttgcg
     1021 catccgggtg atggactgca gcaccgggcc cgtcccgaga gccgaggggc cgttgagggt
     1081 gttgaacagg aagggcgatc cctgggtgac gttgagccgc tgatcggtgg tcatgacgta
     1141 gccgttggcg tccacgtagg agccggcctt ggcgatgttg gaggccagga cgttctgttc
     1201 ggcctgctgg agcagcaggc cgttgagggt ggtgttgatc ccgaaaaagc tgttgatcat
     1261 gcgttcctcg ttataccgtt cggttgacga aggccggcgc ggcctgggtc tgcggcttct
     1321 cggcgcccgc ccgggccgcc gagggccgcc ccgcggtgcg gacggccttc aaggtgaaat
     1381 tcacgtagga caaggcgttg cgcaaaagtt tgtcgttgcc ctggttcaac gcttccaact
     1441 cccgcgcggt cttggccagg agcagggcgg actcccggaa aacctccgac acttcccccg
     1501 gcgccgcttg gatggcttgg gccaggtcca tcttcttcac gcccagcgtc ttgccgatct
     1561 ccgccagcaa ctcgttgcgg cggcccgcca gcgggcccaa ttggaacacc tgcttctcgc
     1621 ccgaccggac gttgtcgggc aaggcctcga tcttcgagga caccaaaata tcccgttgtt
     1681 ccccggccaa ggccgacagc cggcggtaac attccgtttc cttggccatc acctcggcca
     1741 gttcccggaa aaggggcgcg gtcatcacag ccccgggttg atgatctgcg aatccagcaa
     1801 ctggctggcc aagctctggt ggtcgatctt gaaatccggg gacttcacct gctgc
//
LOCUS       DASTJJ010000378         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1153935, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000378 DASTJJ010000000
VERSION     DASTJJ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153935"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..782)
                     /locus_tag="VFR02_04720"
     CDS             complement(12..782)
                     /locus_tag="VFR02_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET9869780.1"
                     /translation="MTEWLLLPALLGLVSLDVSAVGQFMVSRPIVTGPLVGFLLGQPA
                     LGLEMGALIEIIWIGDLPVGAHLPIDIMMLAGVSTAFACELAGQGHFPPEAVMTYAIG
                     ICIPLAALSTEAEMLLRRFHVRWVHFAQKLASEGHDRLFGWLNWIVLGELFVKGFLTA
                     LVSLFVAHLTGRLFLILPPKVLQGLYYAYWLLLALGCSAVIDLMVERQTVAYLILSIA
                     AFMTLAIFLHLPGVLLVGIALLSGFVLSLFYMRRGEAR"
     gene            complement(779..>1080)
                     /locus_tag="VFR02_04725"
     CDS             complement(779..>1080)
                     /locus_tag="VFR02_04725"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HET9869781.1"
                     /translation="AYYVKGRELRERALNSMGREEGVTEAGMTDGWLKLQIDFKSPRP
                     VDFFRYPVETISQSEGGFERVYQGSCLLFGQEVVLGPHQAFETELTAQWREAAGS"
BASE COUNT          232 a          371 c          336 g          141 t
ORIGIN      
        1 gccgtcgggg ctcaccgcgc ctccccccgc ctcatataga acaggctcaa cacgaaaccc
       61 gacaggagcg cgatccccac caagagcacc ccgggcaggt gcaggaagat ggccaaggtc
      121 atgaaggccg cgatggacag gatcaggtac gccacggtct ggcgttccac catcaggtcg
      181 atgaccgccg agcaacccag ggccaggagc agccaatagg cgtagtaaag cccctgcagc
      241 accttgggcg gcaggatgag gaacaaccgg cccgtcaaat gggccacgaa gaggctgacc
      301 agggccgtca agaacccctt cacgaagagc tcgcccagga cgatccaatt gagccacccg
      361 aagaggcggt catgcccctc ggaggccagt ttctgcgcga aatggaccca gcgcacgtgg
      421 aaccggcgca gcagcatttc ggcctccgtg ctgagcgccg ccagggggat gcagatcccg
      481 atggcgtagg tcatgaccgc ctcagggggg aaatgcccct ggcccgccag ctcgcaggcg
      541 aaggccgtgg acaccccggc gagcatcatg atgtcgatgg gcaggtgggc ccccacggga
      601 aggtcgccga tccagatgat ctcgatgagc gcgcccatct ccaaccctaa ggcgggctgg
      661 cccaacagga aacccaccag gggcccggtc acgatggggc gggacaccat gaattggccg
      721 accgccgaga catccaggct caccaaaccc aacagcgcgg gaagcaacag ccactcggtc
      781 atgatccggc cgcctcccgc cactgggccg tcaattcggt ctcgaaggcc tggtgggggc
      841 ccaggaccac ttcctgcccg aacaacaggc aggaaccctg gtagacccgc tcgaacccgc
      901 cctcggactg ggaaatggtc tccaccggat agcggaaaaa atccaccggg cgggggctct
      961 tgaagtcgat ctggagcttg agccacccgt cggtcatgcc cgcttcggtg acgccctcct
     1021 cccggcccat gctgttgagg gcccgttccc gcaactcccg gcccttgacg taataggcgc
//
LOCUS       DASTJJ010000379         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_53527, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000379 DASTJJ010000000
VERSION     DASTJJ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53527"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFR02_04730"
     CDS             <1..869
                     /locus_tag="VFR02_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9869782.1"
                     /translation="ARQSLLPQLVPPGTFTNALTWNSSIFQVATVAGPALGGLLLLGL
                     GYTRLCLLEALFEMVFFALALAVRTSPRAFRREPVSLRSLLSGFRFVRDNRLVLATIT
                     MDLFVVVLGGATALLPAFSSDILRCGAVGYGFLQAAMPAGAFGMALLLAHRPPMRRAG
                     RSLLWAVAGFGLATVVFGLSRSYWLSFLALAVAGALDNVSVIVRSTLVQVLTPDAMRG
                     RVTAVSYIFIHSSNELGGFESGATAALLGPVGSVVLGGLGSVLVVLAVARIWPEVARF
                     GALEKAGRKGRS"
     gene            complement(873..1319)
                     /locus_tag="VFR02_04735"
     CDS             complement(873..1319)
                     /locus_tag="VFR02_04735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET9869783.1"
                     /translation="MTPFSLRASRAGDRRALALVYQAAFSGEGPWTLRAAEARVAQVL
                     KSKQPAWTACVYGQPVGFLFLSLREGHSGPYGEVTELAVHPYFQGQGIGRKLVRVART
                     SRWGRKLKTLYGLVYHGPAEGFLRQNGFKPSRRAVVMSLHGSRGKK"
     gene            complement(1352..>1805)
                     /locus_tag="VFR02_04740"
     CDS             complement(1352..>1805)
                     /locus_tag="VFR02_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869784.1"
                     /translation="TPFDFYDFPLSHSLVMSLVWAAGFGLVYFILRGDKRSALVLAAL
                     VVSHWFLDVIVHRPDLPLTPLARHFWGLGVWNSEVGTLLAEYGLLAAGAWIYLKGTKS
                     KDPMGNWVFYGLVFTLAAFYLISFLAPPPSNPEMVAWGGIIVIWGFIA"
BASE COUNT          281 a          621 c          597 g          306 t
ORIGIN      
        1 cggcccggca gtcgctgctg ccccagctcg tccccccggg gaccttcacc aacgccctca
       61 cttggaacag cagcattttc caagtggcca ccgtggcggg acccgcgctg ggggggctgt
      121 tgcttttggg cctgggctat acccggctct gccttttgga ggcgctcttc gagatggtct
      181 tcttcgccct ggccctggcc gtgcggacct ccccccgggc cttccgccgg gaaccggtca
      241 gcctgcggtc cctcctgagc ggcttccgtt tcgtgcgcga caaccgcctg gtcctggcca
      301 ccatcaccat ggaccttttc gtggtcgtcc tggggggcgc cacggcgctc ttgcccgcct
      361 tctcgtccga catcctgcgt tgcggcgccg tgggttacgg tttcctgcag gccgccatgc
      421 cggcgggcgc cttcgggatg gccctgctgc tggcccaccg cccccccatg cggcgggcgg
      481 ggcgttcctt gctttgggcg gtggcgggat tcgggttggc cacggtggtc ttcggcctct
      541 cgcggtccta ctggctctcc ttcttggcgc tggccgtcgc cggcgccctg gacaatgtca
      601 gcgtgatcgt gcggtccacc ctggtgcagg tgctcacacc cgacgccatg cgggggcggg
      661 tcaccgccgt cagctatatc ttcatccact cctccaacga gctggggggg ttcgagtccg
      721 gcgccacggc cgccctgctg ggcccggtgg gatcggtggt gctggggggc ctggggagcg
      781 tcctggtggt gttggcggtg gccaggatct ggcccgaagt ggcccgtttc ggcgcgttgg
      841 agaaggctgg ccgcaagggc cggtcctgag ctttactttt ttccccggct tccatgaagg
      901 gacatgacga ccgcgcggcg ggagggtttg aacccgtttt ggcgcaggaa cccctcggcc
      961 gggccgtgat agaccaagcc atagagggtc ttcaacttgc gtccccagcg ggacgtccgg
     1021 gccacccgca ccaacttgcg gccgatgccc tggccttgaa aataaggatg cacggccagt
     1081 tcggtcactt ccccataggg ccccgagtgc ccttcgcgca gcgacagaaa aagaaaaccc
     1141 accggctggc cgtaaacgca ggccgtccag gcgggttgtt tggacttcaa cacctgggcc
     1201 acccgtgcct cggcggcccg caaggtccag ggcccctcgc ccgagaaggc cgcctgatag
     1261 accaaggcca gggcccggcg gtcgccggcc cgggaagccc gcagggagaa gggcgtcaaa
     1321 ccgctttccg catccgatcg agccaccaac ctcaggcgat gaagccccag atgacgatga
     1381 tgccgcccca ggccaccatc tcggggttgg agggcggcgg cgccaggaag ctgatcaggt
     1441 agaaggccgc caaggtgaag accagcccat agaacaccca gttccccatc gggtccttgg
     1501 acttggtgcc cttcaggtag atccaagccc cggcggccag caggccatat tcggccaaga
     1561 gggtccccac ctcggaattc cacacgccca ggccccagaa atgccgggcc aagggggtca
     1621 ggggcaggtc cggccggtgc acgatgacgt ccaggaacca atggctcacc accagggccg
     1681 ccagcaccag ggcggaacgc ttgtcccccc ggaggatgaa atagaccagg ccgaaccccg
     1741 cggcccagac cagcgacatc accaaactgt ggctgagcgg gaaatcataa aagtcgaagg
     1801 gcgtg
//
LOCUS       DASTJJ010000380         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_53583, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000380 DASTJJ010000000
VERSION     DASTJJ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53583"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFR02_04745"
     CDS             complement(<1..306)
                     /locus_tag="VFR02_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9869785.1"
                     /translation="MGKRIGVVMGDIPGGWVAEAVGLGSDVAVEEGVAVGVPVIVGLG
                     VGERVGVGVAVRVGEDEGVGLAVGVWVGKGVAVGVLVAEGEGLALGVAVAVGVPVRVG
                     "
     gene            326..559
                     /locus_tag="VFR02_04750"
     CDS             326..559
                     /locus_tag="VFR02_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869786.1"
                     /translation="MTVDFDLRDASAWVRVEVFTTAFRKVDEVDLSNLPAGPQRVTLP
                     LTDRHGAALANGLYYVLVLNSQGRAIGKLLVAR"
     gene            874..>1261
                     /locus_tag="VFR02_04755"
     CDS             874..>1261
                     /locus_tag="VFR02_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869787.1"
                     /translation="MKTEFRLLAGLILWGSAPALWAGTATIDATVTDQYIRGFGACSA
                     WHESAFSSQLATWFWDSTGMTGNNPNGIGLSLLRCHIPETNSSTGNPSDPGEVAVMKQ
                     AAGLGVTQIWLAEWTPPTAYKTAGATY"
BASE COUNT          221 a          492 c          346 g          202 t
ORIGIN      
        1 cccgacccga acaggcaccc ccaccgctac ggccacgccc agcgcgagcc cctccccttc
       61 ggccactagg acccctaccg ccacgccttt cccgacccaa accccgaccg cgagccccac
      121 gccttcgtct tctccgaccc ggacggcgac ccccacgccc acccgttcgc cgacgccaag
      181 ccccacgatg accggcacgc cgacggccac gccttcctcg accgccacgt ccgaacccag
      241 cccgacggct tcggccaccc agccgcccgg gatgtcgccc atcaccacgc cgatcctttt
      301 ccccaacccg gccacgggaa gccaagtgac ggtggacttc gacctgcggg acgcttccgc
      361 ctgggtgcgg gtggaggtct tcacgacggc cttccgcaag gtggatgagg tggacctctc
      421 caacctgccc gccgggcccc agcgcgtgac cctgcccctg accgaccgtc acggggcggc
      481 gctggccaac ggcctttact acgtgctggt gctcaattcc cagggccgcg ccatcgggaa
      541 actattggtc gccaggtaaa agacggcggg agatccgatc cttccggatc ccccgccgcg
      601 cgtcttttgt tccacccgcc tcaaggtaaa cgcattttcc ttcctggaag catcccttcc
      661 gccccggccc aagttgaacg accaagccct ttgttgaacc ggtccgttcc cgaacggcga
      721 aatagaaggc aacttatttg acgtttaagg gtaaccgctc ttcgccgccc gcggcgcggc
      781 agggcccgat ccatgccata gtccccccac ggggaggacc gtcttgtcgt cctccgtccg
      841 ccaccaaaat cgtttgaaga cgaaagaggg accatgaaga ccgaattccg tttgctggct
      901 ggtttgatcc tgtggggttc cgcccccgcc ctgtgggccg gcacggccac catcgacgcc
      961 acggtcaccg accagtacat ccggggcttc ggggcctgca gcgcctggca cgaaagcgcc
     1021 ttttccagcc aattggccac ttggttctgg gattccaccg gcatgacggg caacaacccc
     1081 aacggcatcg gcctttccct gctgcgctgc catatccccg agaccaattc atccacgggc
     1141 aacccctcgg acccggggga agtggccgtc atgaagcagg ccgcgggcct gggggtcacc
     1201 cagatctggc tggccgaatg gacgccgccg accgcttata aaaccgccgg ggccacctac
     1261 g
//
LOCUS       DASTJJ010000381         1322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_665098, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000381 DASTJJ010000000
VERSION     DASTJJ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665098"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..914)
                     /locus_tag="VFR02_04760"
     CDS             complement(111..914)
                     /locus_tag="VFR02_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET9869788.1"
                     /translation="MSGAGSLAALGFLAIGLGLSALAMLALWALQLRTKNAAVMDAGW
                     ALGVAALAALDAWQGWGWGPRRALALGLAGAWGLRLGLYLLATRVWGRPEAGHYRRLR
                     LEWKKKAKSNFLVFFQFQAFLTAFFSLPFLLASLNPRPGFSALEIAAVLLWVLAFTGV
                     WLADRQLHRFKQDRKNRGKTCRAGLWGHSRHPNYFFEWLLWCANALFALASPFGWLAL
                     LCPALLLYFLFKMTGIPATEAQALRTKRDYRAYQRATSAFVPWFKKRKA"
     gene            complement(911..1246)
                     /locus_tag="VFR02_04765"
     CDS             complement(911..1246)
                     /locus_tag="VFR02_04765"
                     /inference="COORDINATES: protein motif:HMM:NF018991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HET9869789.1"
                     /translation="MTQTLKVFYAAVLLAMVFMTAWASLKGGNVLPATAVLWGEPWGR
                     ATLVDAYFAFTTIFLWVCSVEKKASTRVLWFALIMTLGNLAVASFMLRRLSRLKPGGD
                     LRAFLTEAL"
BASE COUNT          282 a          417 c          460 g          163 t
ORIGIN      
        1 cttcgatcgc ctcaggcaag tgaaaacctc cgcaaaggga tgaaagcggg ggacaaatta
       61 agggggaagg gggatcgatg tcaagcgggc gccgggcgcc cgccgcggga tcaggccttg
      121 cgcttcttga accagggcac gaaggcgctg gtggcccgct gataggcccg gtaatcccgt
      181 ttggtgcgca gggcctgggc ctcggtggcc gggatgcccg tcatcttgaa gaggaaataa
      241 aggagcaacg ccgggcagag aagcgccaac cagccgaaag gcgaggccag cgcgaaaagc
      301 gcgttggcgc accaaagcag ccactcgaag aaatagttgg ggtggcggga atggccccaa
      361 agcccggcgc ggcaggtctt accccggttc ttgcggtcct gtttgaaccg gtgcagctgc
      421 cggtcggcca gccatacccc ggtgaaggcc aggacccaga ggaggaccgc cgcgatctcc
      481 aaggcggaaa aaccgggcct ggggttgagc gaggccaaaa ggaagggcag cgagaagaag
      541 gccgtcagga aggcctggaa ctggaaaaag accaggaagt tcgacttcgc ctttttcttc
      601 cattccagcc gcagccgccg gtaatggccc gcctcgggcc gcccccacac gcgggtggcg
      661 agcaggtaca ggcccagccg caggccccag gcgccggcca gccccagggc caaggccctg
      721 cgcggccccc agccccaacc ctgccaggcg tccaaggcgg ccagggccgc gacccccaag
      781 gcccagcccg cgtccatcac ggcggcgttc ttggtgcgca actgcagggc ccaaagggcc
      841 agcatggcca gggccgacag ccccaggccg atggccagga accccaaggc cgccaaggat
      901 ccggcgccgc tcatagggcc tccgtcagga aggcgcgcag gtccccgccg ggcttgaggc
      961 gggaaagcct ccgcaacatg aaggaggcga cggccaggtt gcccagggtc atgatgagcg
     1021 cgaaccagag gacgcgggtg gaggcctttt tttccaccga gcagacccaa aggaagatgg
     1081 tggtgaaggc gaaataggcg tccacgagcg tcgcccggcc ccagggttcc ccccagagga
     1141 cggcggtggc gggcaggacg ttgccgccct tcaacgaagc ccaagcggtc atgaagacca
     1201 tggccaacag cacggcggcg taaaagacct tcaaggtttg ggtcatggat cctccgggat
     1261 gcggataaaa aaagggaggc ggggcttcgc ccacctccct tttggaacgg atcggccgcc
     1321 tt
//
LOCUS       DASTJJ010000382         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1032456, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000382 DASTJJ010000000
VERSION     DASTJJ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032456"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /gene="uvrC"
                     /locus_tag="VFR02_04770"
     CDS             <1..1502
                     /gene="uvrC"
                     /locus_tag="VFR02_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9869790.1"
                     /translation="DDKSYPYLKLTKEPFPRVFISRRPTADGSKYYGPYPNLKLREIL
                     KLVYRYFNIRDCDLEIDGKAERACLSYQIKQCPAPCIGAVDQAGYAAVVERVKWFLEG
                     KHEPLMERLRAEMLRASDRQEFEEAAKLRDLLGSIEQMQNGYAVITAEKLDLDVVALT
                     AGLGKALASVLQVRQGKVVDHVKLVLENELDQPLPEVLPLFLRQYYAPGVFVPDEVLV
                     ARDFPLDEETARWAAAFPDKPVAVKNAQGGWRADLMEIAERNVLSALKEDLQQTEVLK
                     ELQGLLRLKAVPRNIACFDISTLQGSFTVGSAVFFRDGVPEKGRYRKFRIKELEGQDD
                     FRSHQEMMRRYLALVEREGNPLPDLLLIDGGKGQLSAVKAVLDQALGGGYGLASLAKR
                     EEEVFLPGASRPVDFTGHLKARYLLQRVRDESHRFAVGYHRIIRDRSAVTSILRGVKG
                     IGPTRLRALLNSFESVEKVKAASLEDLQAVPHFSKELANKLYQHFHTVS"
     gene            1661..>2273
                     /locus_tag="VFR02_04775"
     CDS             1661..>2273
                     /locus_tag="VFR02_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869791.1"
                     /translation="MANFTFRGSILAELVSRTLFVGIIILFVYGGFSYYISKETFDGE
                     MGERLVDIARLTAGQLKGEWVPFLDPGVGVYRSFQRTLADIREQSQAKDIFVLDRDGR
                     VLVDAAQDYQFRETDWLKDLDPEPFLKARRGLSSASILFPEKDGSIYKIGYSPVRDPS
                     GAVVAVVGVEASAKFMDGLNQFARILFIFGLVCLALMGGLLYAF"
BASE COUNT          387 a          733 c          755 g          398 t
ORIGIN      
        1 gggacgacaa gagctacccc tacctgaagc tcaccaagga gccctttccc cgggtcttca
       61 tttcccgccg gcccacggcg gacgggtcca agtattacgg cccctacccc aacctgaagc
      121 tgcgggagat cctcaagctg gtctaccgct atttcaacat ccgggactgc gacctggaga
      181 tcgacggcaa ggccgagcgg gcctgcctga gctaccagat caagcaatgc ccggcgccct
      241 gcatcggggc cgtggaccag gcgggctatg ccgccgtggt ggagcgggtc aagtggttcc
      301 tggaagggaa gcatgagccc ctgatggagc gcctgcgcgc cgagatgctg cgggcctcgg
      361 accggcagga gttcgaggag gcggccaagt tgcgcgacct gctggggtcc atcgagcaga
      421 tgcagaacgg ctacgcggtc atcaccgcgg agaagctgga cctggacgtg gtggccctga
      481 ccgccggcct gggcaaggcg ctggcgtccg tgctgcaggt gcggcagggc aaggtggtgg
      541 accacgtcaa gctggtgctg gagaacgagc tggaccagcc cctgcccgag gtgctgcccc
      601 ttttcctgcg ccagtactac gcgcccgggg tcttcgtgcc cgacgaggtg ctggtggccc
      661 gcgacttccc gctggacgag gagaccgccc gatgggccgc cgccttcccg gacaagcccg
      721 tggcggtcaa gaacgcccaa gggggctggc gcgccgacct gatggagatc gccgagcgca
      781 acgtgctgtc ggccctgaag gaggacctgc agcagaccga ggtgctgaag gagctgcagg
      841 ggctgcttcg cctcaaggcc gtgccccgca acatcgcctg cttcgacatc tccaccctgc
      901 agggcagctt cacggtgggc tcggcggtct ttttccgaga cggcgtgccc gagaaggggc
      961 ggtaccgcaa gttccgcatc aaggaactgg aaggccagga cgatttccgc tcgcaccagg
     1021 agatgatgcg ccgttacctg gcgctggtgg agcgggaagg gaaccccttg cccgacctgc
     1081 tgctcatcga cgggggcaag ggccagctct cggccgtgaa ggccgtgctg gaccaggccc
     1141 tggggggcgg gtacggcctg gcgtccctgg ccaagcggga agaggaggtc ttcctgcccg
     1201 gggcatcccg gccggtggac ttcacgggcc acctgaaggc ccgctacctg ctgcagcggg
     1261 tccgggacga gtcccaccgt ttcgccgtgg gctaccaccg catcatccgg gaccgcagcg
     1321 cggtcacctc gatcctgcgg ggcgtgaagg gcatcggccc cacccggctt cgggccctgt
     1381 tgaacagctt cgagtcggtg gaaaaggtca aggcggccag tttggaggac ctgcaggccg
     1441 taccccactt ttccaaggaa ttggccaata agctttacca gcatttccac acggtttcct
     1501 gacccccggg ttttgatgac gcttcggcgg ccctcccgct ttttttaaca tccctagtca
     1561 aaaattttac aaagttcccg agcggtgtta tgattcaatc atcgcgtcag tttcatgaca
     1621 tcgtcctctc cgcgcggcgg agagtgaaca ggcgggcagg atggccaact tcacattccg
     1681 gggcagcatc ctggccgaat tggtgagccg cacgctcttc gtgggcatca tcatcctctt
     1741 cgtgtacggc ggtttttcct actacatctc gaaagagacc ttcgatgggg agatgggcga
     1801 gcgcctggtg gacatcgccc ggctcaccgc cgggcaactc aagggcgaat gggtgccctt
     1861 cctggatcca ggggtcgggg tctaccggtc cttccagcgg accctggcgg atatccggga
     1921 gcagagccag gccaaggaca tcttcgtgct ggaccgggac ggccgggtcc tggtggacgc
     1981 cgcccaggac taccagttcc gggaaaccga ctggctcaag gacctggacc ccgaaccctt
     2041 cctcaaggcc cggcggggcc tttccagcgc ctccatcctc ttcccggaga aggacggcag
     2101 catctataag atcggctaca gcccggtgcg ggacccctcg ggcgccgtgg tggccgtcgt
     2161 gggcgtggaa gccagcgcca agttcatgga cgggctcaac cagttcgccc gcatcctctt
     2221 catcttcggc ctggtctgcc tggcgctcat gggcgggttg ctttacgcct tcg
//
LOCUS       DASTJJ010000383         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_910287, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000383 DASTJJ010000000
VERSION     DASTJJ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910287"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="bshB1"
                     /locus_tag="VFR02_04780"
     CDS             <1..657
                     /gene="bshB1"
                     /locus_tag="VFR02_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HET9869792.1"
                     /translation="AHPDDVEIAMGGTLLALKARGAKVAVCHLTDGEPTPFGTHALRL
                     KEAEKAARVLGLDDYRILPLKNRSLQDTLPARKKVAEAIRLYRPRILVAPYWVDAHPD
                     HWNASQLVEAARFWAKLVKTDMKGEPWYPPKLYYHLCSHLRPNVQPSFIVDTSAHHAK
                     KIQAVRCYESQFLKKPGSRALEHIDVVNRYFGAMIGVTTGEPFFSKENLGVSDPRLFL
                     "
     gene            787..>1513
                     /gene="recA"
                     /locus_tag="VFR02_04785"
     CDS             787..>1513
                     /gene="recA"
                     /locus_tag="VFR02_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9869793.1"
                     /translation="MAASEKQKALDMAILQIEKDHGKGAIMRLGEAKVQDVKAISTGA
                     MSLDIATGIGGVPRGRIIEIYGPESSGKTTLTLHIVAEAQRAGGTAVFIDAEHAMDPV
                     YAAKLGVNVNELLISQPDSAEEALQIADTLIRSGAIDVLVVDSVAALAPRAELEGEMG
                     DAHVGLQARLMSQALRKLTALLSKSHTSAIFINQIREKIGVMFGNPETTPGGRALKFY
                     SSMRLDIRRIAAIKDGDQVVGSRT"
BASE COUNT          298 a          518 c          450 g          247 t
ORIGIN      
        1 gcccacccgg atgacgtgga gatcgccatg ggaggcaccc tcctggccct caaagcccgg
       61 ggcgccaagg tggcggtctg ccacctgacc gacggggagc ccaccccctt tgggacccac
      121 gccctgcggc tcaaggaagc cgaaaaggcc gcccgcgtcc tgggcttgga cgattaccgg
      181 atcctacccc ttaagaaccg ctcgctccaa gacaccctgc cggcccgaaa gaaagtggcc
      241 gaggccatcc ggctttaccg gccccgcatc ctggtggccc cctattgggt ggacgcccat
      301 ccggaccatt ggaacgcctc ccaactcgtc gaggcggcgc gcttttgggc caagctggtc
      361 aagaccgaca tgaagggcga gccctggtat ccgcccaagc tttattacca cctctgctcg
      421 cacctgcggc ccaacgtgca accttccttc atcgtggaca cctcggccca tcacgcgaaa
      481 aagatccagg ccgtgcggtg ctacgaaagc cagttcctca agaagcccgg cagccgggcc
      541 ctggagcaca tcgacgtggt gaaccgttat tttggcgcca tgatcggcgt gacgaccggc
      601 gagcctttct tcagcaagga aaacctgggg gtctcagacc cccgcctgtt cctttaaaaa
      661 taccccaaaa aaacacttgc aaccggtggt atacgcctgt attctttttt ctcaacgggg
      721 ccccaggggg tgccgttggg gggcggaggc ccgaaaggtt ccgccattaa ttccaaggag
      781 agtctcatgg cggcgagcga gaaacaaaaa gcgttggata tggcgatcct gcagatcgaa
      841 aaagaccacg gcaagggcgc catcatgcgc ctgggcgagg ccaaggtgca ggacgtgaag
      901 gccatctcca cgggggccat gtccctggac atcgccaccg gcatcggcgg ggtgccccgg
      961 ggccgcatca tcgagatcta cgggcccgaa tcctcgggta agaccaccct gaccctgcat
     1021 atcgtggccg aggcccaacg ggccggcggc acggccgtct tcatcgacgc cgaacacgcc
     1081 atggacccgg tctacgccgc caaactgggc gtgaacgtca atgaactgct catttcccag
     1141 cccgattcgg ccgaagaggc cctccagatc gccgacaccc tcatccgttc cggcgccatc
     1201 gacgtgctgg tggtcgactc ggtggcggcg ctggcccccc gggccgagct ggaaggggag
     1261 atgggcgacg cccacgtggg gctccaggcc cgtttgatga gccaggccct gcgcaaattg
     1321 acggccctgc tttccaagtc ccacacctcc gccatcttca tcaaccagat ccgcgagaag
     1381 atcggggtca tgttcggcaa cccggagacc acgccgggcg gccgggcgct caagttctat
     1441 tcctccatgc gcctggacat ccgccgtatc gccgccatca aggatggcga ccaggttgtc
     1501 ggctcgcgca cca
//
LOCUS       DASTJJ010000384         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1521819, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000384 DASTJJ010000000
VERSION     DASTJJ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521819"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFR02_04790"
     CDS             complement(<1..881)
                     /locus_tag="VFR02_04790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9869794.1"
                     /translation="MTKTDPKPRPRVAILRGPFLNSFEMQTYELLKGEFDITAFTPHQ
                     TYFDTSGIDLPQESLWCPIAGKTPFEREKRKWQALRDRLTGRTHSFCGLRDRLEGFDL
                     YHIMDQYFCFSYEAALAKRRHGGKLVVTQYENIPFLNDHKAMERQIKRSVREQADLFL
                     AMSGGAREVLLQEGVPEPKIEPFSGAVDTKRFCPGARDRALARELGLTGASFTLLYVG
                     RLAKSKGVFTLLKAAEVLAREDAGFRLLLVGKDEEGVGDWVAQRGLERCVRLAGFVAY
                     PQMPRYYRLADAFVLPSL"
     gene            complement(878..>1150)
                     /locus_tag="VFR02_04795"
     CDS             complement(878..>1150)
                     /locus_tag="VFR02_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9869795.1"
                     /translation="CDLFVGNDSGLTHLASGRGARVVVLFGSTTPALGFAPWGPHTVV
                     EVTGLPCRPCGVHGRSSCPEGHFKCMLELTVDAVETAAKRSLRRAR"
BASE COUNT          195 a          400 c          374 g          181 t
ORIGIN      
        1 aggctgggca gcacgaaggc gtcggccaag cggtaatagc ggggcatctg gggataggcc
       61 acgaaccccg ccaggcgcac acaccgctcc aacccccgct gggccaccca atcgcccacg
      121 ccctcctcgt ccttgccgac caacaggagc cggaaccccg cgtcctcccg ggccaggacc
      181 tccgccgcct tcaagagcgt gaagaccccc ttgctcttgg ccaggcgccc cacgtaaagc
      241 agcgtgaagg aagcccccgt caggcccagt tcccgcgcca gggcccggtc ccgggccccg
      301 gggcaaaagc gcttggtgtc caccgcgccg ctgaagggtt cgatcttcgg ctccggcacg
      361 ccttcctgca acaggacctc ccgggcgccc cccgacatgg ccaggaagag gtccgcctgt
      421 tcccgcaccg accgtttgat ctgccgctcc atggccttgt ggtcgttcag gaaggggata
      481 ttctcatatt gggtcaccac cagcttgccc ccgtggcggc gtttggccag ggccgcctca
      541 tagctgaaac agaaatactg gtccatgatg tggtagaggt cgaacccttc caggcggtcc
      601 cggaggccgc agaaggaatg ggtccggccc gtgaggcggt cccggagggc ctgccatttg
      661 cgtttttcgc gctcgaaagg ggtcttgccc gcgatggggc accagaggct ttcctgcggc
      721 aggtcgatcc ccgaggtgtc gaagtaggtc tggtggggcg tgaaggcggt gatgtcgaat
      781 tcccccttca gcagctcata ggtctgcatc tcgaaggaat tcaggaaagg tccccgcagg
      841 atggccaccc gggggcgggg tttcgggtcg gtcttcgtca ccgggccctc cggagggacc
      901 gtttggcggc ggtctccacc gcgtccaccg tcaattccag catgcatttg aaatgcccct
      961 cggggcagga cgagcgtccg tgcacgccgc aaggacggca gggcagtccc gtcacctcca
     1021 ccactgtgtg gggcccccag ggcgcgaaac ccagcgccgg cgtggtggac ccgaacagca
     1081 ccaccacccg ggcgccccgg cccgaggcca ggtgggtgag ccccgaatcg ttgcccacga
     1141 agaggtcgca
//
LOCUS       DASTJJ010000385         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1276321, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000385 DASTJJ010000000
VERSION     DASTJJ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276321"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1310)
                     /locus_tag="VFR02_04800"
     CDS             complement(156..1310)
                     /locus_tag="VFR02_04800"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9869796.1"
                     /translation="MKKRIKRPKLLKTVPPTAYAHFGLSLDPFTTLSLQAHNLDYFIG
                     REALADRLASALFSLGNVGLAGEPGVGKSSLMQALLSRVPSAFHPILIGVPLDDARYF
                     LAELLREILVRVPKAPGLNLKEVGRRMESEALSKNTLLALVKSIAAKSPKPLLVFVDD
                     LEKIKGDRVQHLTRSERTLQLLEELKPLLETPRMAFAVSLQEEFHAKVAAVVKEGAEP
                     TVLGLFKNIVLVERFSGEEMRGLLARRLERAGYAGGLGGFLEPEALTLALALSGGNPR
                     RVLFLLSEGMYRGFRRGGSRVEFQDLFEAVNEHLKLDLVCKKLLYFLAKSGRATAANP
                     DLQAFMGLDTVSIARRLEVLTKNRLAEMVDVADGSRVFALPGSIPRPEAS"
     gene            complement(1335..1724)
                     /locus_tag="VFR02_04805"
     CDS             complement(1335..1724)
                     /locus_tag="VFR02_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9869797.1"
                     /translation="MVAEVLLDTSVWIDFFRKPEGPTGEQIIKHLKKGTVSTCGVVVF
                     EVLQGAADPAEFAFLEENLKGLHFLEADAASYFEAGRTSYGLRKRGVTLPLSDILIAT
                     LAISNRQVLWTKDQHFKKIKALHSHFL"
     gene            complement(1714..1920)
                     /locus_tag="VFR02_04810"
     CDS             complement(1714..1920)
                     /locus_tag="VFR02_04810"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HET9869798.1"
                     /translation="MRTTLELSNHLIQELMAVEKWDSKTQAIEAAIEDFVRRRRLDNL
                     ISARGRLKVKDVSAELRKLETHGR"
     gene            complement(2085..>2594)
                     /locus_tag="VFR02_04815"
     CDS             complement(2085..>2594)
                     /locus_tag="VFR02_04815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HET9869799.1"
                     /translation="YVGYEFETKGFDVSFKGIIDGFEDLGRDLICKREKQTVIVQCKM
                     WAHYKTVYEKHIFQFFGTVFQYKDEHPYEHVEAQFFTTTKLSELARKFSKALGITLRE
                     GHEFEKNYPCIKCNVSEVTGEKIYHLPFDQQYDKVKIRNPGEFYCRKVEEAEDSGFRR
                     AFRYRGKQD"
BASE COUNT          546 a          788 c          733 g          527 t
ORIGIN      
        1 ccggccccgg gtcattttcc ccttccacca gtaaaggctg cgcacccccg gcaaaaggtt
       61 ggcgcaggcc gccagcttcc ggccgacggc caggcgggcc agacggtccc ccgaggcgcg
      121 gtcggggacg gtgaccaaga ccaccagggc ggcgctcacg aggcctccgg acgcgggatc
      181 gacccgggca gggcgaagac ccgggaaccg tcggccacgt ccaccatctc ggccaggcgg
      241 ttcttggtga gcacctccag gcgccgggcg atggagacgg tgtccaggcc catgaaggcc
      301 tgcaggtcgg ggttggccgc cgtggcccgg cccgacttgg ccaggaaata gaggagcttc
      361 ttgcagacca ggtcgagctt caggtgctcg ttgacggcct cgaaaaggtc ctggaattcc
      421 acccggcttc ccccgcggcg gaagccccgg tacatccctt ccgagagcag gaagaggacc
      481 cgccggggat tgccgcccga aagggcgagc gccagggtca gggcctcggg ttccaagaag
      541 ccccccaggc cgcccgcgta tcccgcccgc tccaggcgcc gggccaggag cccccgcatc
      601 tcctccccgc tgaagcgctc gaccagcacg atgttcttga acaatcccag cacggtgggc
      661 tcggcgcctt ccttcaccac ggcggccacc ttggcgtgga actcctcctg cagggagacg
      721 gcgaaggcca tgcggggcgt ttccagcagc ggcttgagct cctccaaaag ctgcagcgtg
      781 cgctcggagc gggtcaggtg ctgcacccgg tcgcccttga tcttctccag gtcgtccacg
      841 aagaccagca ggggtttggg cgacttggcc gcgatggact tgaccagggc gagcagggtg
      901 ttcttcgaaa gggcctcgga ttccatccgt cggcccactt ccttcaggtt gagccccggc
      961 gccttgggca cccgcaccaa gatctcgcgc aggagctcgg ccaggaaata acgggcgtcg
     1021 tccaagggca cgccgatcag gatgggatga aaggcggacg gcacgcggga aagcagggcc
     1081 tgcatcaggc tgctcttgcc cacgcccggt tccccggcca gccccacgtt gcctagggaa
     1141 aagagcgcgg aggccaaccg gtcggccagc gcctcccggc cgatgaaata gtccaggttg
     1201 tgcgcctgga gggacagggt ggtgaagggg tccagggaaa gcccgaagtg ggcgtaggcc
     1261 gtcgggggga cggtcttgag gagtttcggc cttttaatcc tctttttcat tgtcgtccta
     1321 tgcccggatc gggttcataa aaaatgggaa tggagcgctt taatcttctt gaaatgctgg
     1381 tccttggtcc aaagaacctg gcggttggag atggccagcg tcgcgataag gatgtcgctc
     1441 aagggcaggg tcacaccacg cttgcgcagg ccgtaggaag tccgtccggc ctcgaagtag
     1501 gaggccgcgt cggcttccaa gaaatgaagg cccttcaggt tctcctccaa gaaagcgaat
     1561 tcggccgggt ccgccgcgcc ctgcaggacc tcgaatacca cgaccccgca agtggagact
     1621 gtcccctttt tcaaatgctt gatgatctgc tcgccggtcg ggccctccgg cttccggaag
     1681 aagtcgatcc aaaccgacgt gtcgaggagg acctcagcga ccatgggtct ccagtttcct
     1741 caactcagcc gagacgtcct tcaccttcaa gcggccccgg gcggaaataa ggttgtccag
     1801 ccgccgccgg cgcacgaagt cctcaatggc cgcctcaatg gcctgggtct tggaatccca
     1861 cttctccacg gccatcagct cttggatcaa gtggttggaa agttctaacg tcgtccgcac
     1921 ggccggcccc cagaatccat attaactata tgcatctatt ttatgcatct aaactaccga
     1981 cgtcaaggcg tggatgaaag cgctacccct ttgtttaaac cacctacttg gagggaacag
     2041 gccagcgcga gggggcatca aaaagaccaa actcgtcatt catgtcagtc ttgcttccca
     2101 cggtatctaa aggccctcct aaacccagaa tcttcggcct cttcaacttt tcgacagtaa
     2161 aactcaccag gatttcttat tttgaccttg tcatattgct gatcaaaggg caaatgataa
     2221 atcttttctc ccgtcacttc cgaaacattg catttgatac aaggatagtt tttttcaaat
     2281 tcatgccctt ccctcaatgt tattcccaat gctttggaaa atttcctggc gagctcggaa
     2341 agcttagttg ttgtaaaaaa ttgagcctca acatgctcgt aaggatgctc atctttatat
     2401 tgaaaaacgg ttccaaagaa ctgaaaaatg tgcttttcat aaacagtttt gtaatgcgcc
     2461 cacattttgc attgaacaat tacggtttgt ttttctcttt tgcatattaa atcacggccc
     2521 agatcttcaa aaccgtcaat gattccttta aatgaaacat caaatccctt cgtctcaaac
     2581 tcatatccga cata
//
LOCUS       DASTJJ010000386         1211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_53901, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000386 DASTJJ010000000
VERSION     DASTJJ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53901"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFR02_04820"
     CDS             complement(<1..618)
                     /locus_tag="VFR02_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET9869800.1"
                     /translation="MRSMTGFGRGSAQVKSVQVVAEVKTVNHKYHDISLKIPASYQHL
                     EPDLREMILASVTRGKVDLFLKDLSPLRAKTVEVNEELLSEYLKAAHQATKKLKLSGT
                     LSVESLLRLPDVLVVTEAERPEADQKKAAAQALEEALKSLDKMRQAEGGRLAKDMLER
                     AKEIAQVVQQVRTRHKASVAEKIKAFRDKVAEYLPEPLVDQARLST"
     gene            complement(725..>1211)
                     /locus_tag="VFR02_04825"
     CDS             complement(725..>1211)
                     /locus_tag="VFR02_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869801.1"
                     /translation="PTPSPTDTPTSTVTLSPTATHSRTPTATVTDTRTPTPSKTPTAT
                     PTVTLTPTITDTPGPTHTPTVTPTPNVMLTLDSNYFSPPAPLGMDVRVGKPGGVKIVV
                     YNILGERVRTILDANLPAGLTHLTWSGDNDRGDLVGNALYLILIQTPDGRMVRKVIVL
                     R"
BASE COUNT          154 a          390 c          432 g          235 t
ORIGIN      
        1 ggtggaaagg cgggcctggt ccaccagcgg ctcgggcagg tattcggcca ccttgtcccg
       61 gaaggccttg atcttttcgg ccacggaggc cttgtggcgg gtccgcacct gctggaccac
      121 ctgggcgatc tccttggccc gctccagcat gtccttggcc aaccttcccc cttcggcctg
      181 gcgcatcttg tccagcgact tgagcgcctc ttccagggcc tgggcggccg cctttttctg
      241 gtcggcttcc gggcgctcgg cctcggtcac caccaggacg tcgggaaggc gcagcaggct
      301 ctccaccgac agggtcccgg aaagcttcag ttttttggtg gcttggtgcg cggccttcag
      361 gtactcggaa agcagttcct cgttgacctc gacggtcttg gcccgcaggg gcgagaggtc
      421 cttgaggaag aggtccacct tgccccgggt gaccgaggcc aggatcattt cccgcaggtc
      481 gggctccagg tgctggtagg acgcggggat cttgaggctg atgtcgtggt acttgtggtt
      541 caccgtcttg acttccgcca ccacctggac ggatttcact tgggccgagc ctcggccgaa
      601 accggtcatg cttcgcatgg tcgccacctg ggtctgggtt gcgggccccc gggaaaaccc
      661 cgggacgtcc cgtcaagggg acccatgata gcacgggcgg gcggttccgc cacccggccc
      721 ggccctagcg cagcacgatc accttgcgga ccatccggcc gtcgggggtc tggatcagga
      781 tcaggtacag ggcgttcccc accaggtcgc cccggtcgtt gtcccccgac caggtcaggt
      841 gggtcagccc cgccggcagg ttcgcgtcca ggatggtccg caccctttcc cccaggatgt
      901 tgtagaccac gatcttcacc ccgcccggct tgcccacccg cacgtccatc cccaggggcg
      961 cgggcggcga gaagtagttc gaatccaacg tcagcatcac gttgggcgtc ggggtcacgg
     1021 tgggcgtgtg cgtgggaccc ggcgtgtccg tgatggtggg cgtcagggtc accgtcgggg
     1081 tggcggtggg cgtcttgctg ggcgtcgggg tgcgggtgtc ggtcaccgtg gcggtggggg
     1141 tgcggctatg ggtcgccgtc gggctcaagg tcaccgtgga ggtcggcgtg tccgtcggcg
     1201 acggggtcgg g
//
LOCUS       DASTJJ010000387         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1399594, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000387 DASTJJ010000000
VERSION     DASTJJ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399594"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..708)
                     /locus_tag="VFR02_04830"
     CDS             complement(82..708)
                     /locus_tag="VFR02_04830"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HET9869802.1"
                     /translation="MSNPQHEPTMEEILASIRKIISEDQPDDAAGSPPEPAPADPVPF
                     EPAPQPVVQDDILELTQEVDAPAPEPQPEPMPEQISGPEPEHDVVFQAPEPAPAGDIF
                     SDTTRKAVDDVFASIPQEDEPPAAPAPVAAPLAPVDAATVETVFERAVRESFEPVLKS
                     YLADNSPAIVERMKPLIRVWMDEHFPALLEGAVRAEVERVVKARGAKR"
     gene            complement(889..>1236)
                     /locus_tag="VFR02_04835"
     CDS             complement(889..>1236)
                     /locus_tag="VFR02_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET9869803.1"
                     /translation="SVVALEQDADLVRVASEALERMQGHIAVTQGALIEGFKPDGPYD
                     VIFVNGAIEQRPETLLSQLAEGGRLVAVVKDGAQGRAHLFLKENGVIGRRPDFDADVP
                     LLAGFKKAMGFIF"
BASE COUNT          206 a          399 c          402 g          229 t
ORIGIN      
        1 gtcgagcatg gccgggaaat cgtctttggg ggaagacggg ctttccggct ttttttctgt
       61 caggtcaagc cctgcgcctg tttagcgttt tgcgccgcgc gccttgacca cccgctcgac
      121 ctcggcgcgg acggcccctt ccagcaaggc ggggaaatgc tcgtccatcc atacgcggat
      181 caacggcttc atgcgctcga cgatggcggg ggagttgtcg gccagatagc ttttcagcac
      241 cggctcgaag ctttcgcgca ccgcccgctc gaacacggtt tccaccgtgg cggcatcgac
      301 cggtgccagg ggggcggcca caggcgcggg cgccgccggg ggttcgtctt cctgggggat
      361 ggaggcaaaa acatcgtcca ccgccttgcg ggtggtgtcc gagaagatgt cgccggcggg
      421 ggccggttcg ggggcctgga acaccacatc atgttcgggt tccggccctg atatttgttc
      481 aggcattggt tccggctgcg gttcgggggc gggcgcgtcc acttcctggg tcagttccag
      541 gatgtcgtcc tggaccaccg gctggggcgc cggttcaaac ggaacaggat cggcgggcgc
      601 gggttcgggc ggggaccccg ccgcatcgtc cggctggtct tccgagatga tcttgcggat
      661 ggaggcgaga atctcctcca tcgtcggttc gtgctgcgga ttggacatcg aaagctccac
      721 ttgcggccag gacaggcgcc aaaaccctgg agaaagtaca acgaccctag gacagtgcca
      781 acgtgatggc aaagattagg ttaaccaagc ccttgggccc cccaaatccc cgtttttttc
      841 tcgctcacgc caggcgcgct tcgcacgccg ggcgccgcgg gggcgccgtc aaaaaatgaa
      901 gcccatcgcc tttttgaagc ccgccagcag cgggacatcg gcatcaaaat caggccgccg
      961 accgatcacg ccattttcct tcaggaacag atgggcgcgg ccctgggcgc cgtccttgac
     1021 caccgccacc aggcgcccgc cctccgccag ctgggacaga agggtttcgg ggcgctgttc
     1081 gatggcgccg ttgacgaaga tcacgtcata ggggccatcg ggcttgaaac cctcgatcag
     1141 cgccccttga gtcacggcga tgtggccttg catccgctcc agcgcctcgc tggcgacgcg
     1201 caccagatcg gcatcctgtt ccagcgccac caccga
//
LOCUS       DASTJJ010000388         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_665898, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000388 DASTJJ010000000
VERSION     DASTJJ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665898"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFR02_04840"
     CDS             complement(<1..827)
                     /locus_tag="VFR02_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9869804.1"
                     /translation="MRSEKKEVGSGRGFFRSLAQWVVDHRLPVILGVLAATGFLMSRI
                     GTLKLDADPDLWVPQNDPVIKTTNEIEDTFGGKYAVVVGVTPKQGDIYQPGVLAKVKE
                     MQAKIELMPNVIRANVYSLAARKTKVIHSGPEGMVVRQMMEQVPQTPAQMERLKKDFA
                     TMPAFHKLLVSEDASAVAIVADFKEDKATHMFSPILAQLKAMSAEETKDGTVDVALGG
                     FPLIGAWSEKYMMQMPIYFGLALLVIMGIQFWSFRSFQGMLLPLLTGLFSVFWALGF"
     gene            1048..>1588
                     /locus_tag="VFR02_04845"
     CDS             1048..>1588
                     /locus_tag="VFR02_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9869805.1"
                     /translation="MGKPHSSNPRGRPKAFSEEEALDAAMKVFSDKGYEGASLFDLTR
                     AMGINRTSLYATFGNKESLFRKALDQYTQGMRAFLDGCLKAPTALEGMTQLLTQGVEM
                     FTDPEGPGACLIVRGPLTGTESNKGIQEYFTKKRSMLAQALQERFQKAKEAGELSPGV
                     VPLDLARFYSVVMEGIALQA"
BASE COUNT          312 a          496 c          464 g          316 t
ORIGIN      
        1 atgaagccca gggcccagaa gacgctgaag agccccgtca ggagcggcag caacatgccc
       61 tggaagctgc ggaaggacca gaactggatg cccatgatga ccaacagcgc caggccgaaa
      121 tagatgggca tctgcatcat gtacttctcg gaccaggcgc cgatgagcgg gaagccgccc
      181 agggcgacgt ccaccgtgcc gtccttcgtc tcctcggccg acatggcctt caactgggcc
      241 aggatcgggg agaacatgtg ggtggccttg tcctccttga aatcggccac gatggccacc
      301 gcggaggcgt cctccgacac cagcagcttg tggaaggccg gcatggtggc gaagtccttc
      361 ttcaaccgct ccatctgggc cggggtctgg ggcacctgtt ccatcatctg gcgcaccacc
      421 atgccctcgg ggccgctgtg gatgaccttg gtcttgcggg cggccaggct gtagacgttg
      481 gcgcggatga cgttgggcat cagctcgatc ttggcctgca tctccttgac cttggccaag
      541 accccgggct ggtagatgtc cccctgcttg ggggtcacgc ccaccaccac ggcgtacttg
      601 ccgccgaagg tgtcctcgat ctcgttggtg gtcttgatga ccgggtcatt ctgcgggacc
      661 cagaggtccg ggtccgcgtc cagcttcaag gtcccgatgc ggctcatgag gaaccccgtc
      721 gcggccagga cccccaggat gaccggcaac cggtgatcga ccacccactg ggccagggac
      781 ctgaagaacc ccctgcccga ccccacttcc tttttttccg atctcacgcc agcctcccac
      841 gctataattt cttacctttc ggtacataaa taagccaaaa aacaccattc aggaaagccc
      901 ggagaaggta tgattccccg gtcagcttcg tccaatcgtg tatcttgaaa tatctgtacc
      961 gcttggtacg aatgtagtgg gtggaaaacc gagagtcaag accttattgt atctttcggt
     1021 acataatttt ggtccacagg tgctttgatg ggtaaaccac attccagcaa tccccggggc
     1081 cgccccaagg ccttttcgga ggaagaggcc ctggacgcgg ccatgaaggt cttttcggac
     1141 aagggttatg agggcgcttc cctcttcgac ctgacccggg ccatgggcat caaccgcacc
     1201 agcctctatg ccacctttgg gaacaaggaa tccctcttcc gcaaggccct ggaccaatat
     1261 acccagggca tgcgggcctt tttggacggg tgtttgaagg ccccgaccgc tttggaaggc
     1321 atgacccagc ttttgaccca gggggtcgag atgttcaccg atcccgaagg tcccggcgcc
     1381 tgcctgatcg tgcgggggcc cttgaccggg accgagtcga acaagggcat ccaggaatat
     1441 ttcaccaaga agcgttccat gctggcccaa gccctgcagg aacgcttcca aaaagccaag
     1501 gaggcggggg agctttcccc cggcgttgtt cccttggacc tcgcccggtt ctactcggtg
     1561 gtcatggaag gcatcgccct gcaggcct
//
LOCUS       DASTJJ010000389         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1155004, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000389 DASTJJ010000000
VERSION     DASTJJ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155004"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VFR02_04850"
     CDS             <1..1535
                     /locus_tag="VFR02_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869806.1"
                     /translation="VSVTLGDFDEAYTPLTLWNRDNLDLKYMPEMIAREDDTLKYESF
                     LNHEPEWPFRGARAGTALMWPDSQLLDRFSVSAMTDFVSNGFNDTSGQVFYFGGFSNF
                     IFGANADLKSRRWYLGGPSLQLAMDAYAVILAQPLGTNAPGSIYVQFNPDTWAHHYRI
                     TSLKPSLDFGLGDDASFGGTWEGAEAVYQDDERDNNKVISDYAILAGPYFRFGRSKIS
                     FNYLHVGPYYYSPLAQTRQDDLVPGSGADNAVTPDIFTPPLRSQFFLSAVPRAGDIFS
                     FYDRTQDNTFPYGLSTPNREGGGLDLDVKTLEKDALKIKGSVYFVQEISDNLVVNAAG
                     TGVTTIDGNPAAPTPQRSFAYVNLGPSIDLGPLLQMSTDLVVGVNARYERTDSSLGTL
                     TSTWILGGAQVGILPWWEFAVSYGDQEISGQEAGYGGSTLARYAYLFDNTDLGQYQVF
                     NINGGNQSFRLSSVFKISRNSTLALDWAQTGGNAVPYVGATPTGNSVNNQYAEMTYEI
                     QF"
     gene            1519..2337
                     /locus_tag="VFR02_04855"
     CDS             1519..2337
                     /locus_tag="VFR02_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869807.1"
                     /translation="MRSNSKGAWAAFALASSFLVLAGAGCQNALPGFGAYQATGTTPN
                     TNRVDQFDSGSVSINPKLFENSNPPNYVLRSPGAVTLVNNFASYGSFHPVGSDPNVGL
                     DPGGYVSANCFRMSGAVTDTLDNTFPALDLEVPLENYNFYDANHFTGVKFYMKVANDD
                     TAFHRVFAVPVAQTVPSGTNNGTCPAGPKRCYDHFAADYSGGTGGNWILSSYNFSDLT
                     QLQSGYAPQPYATLTGGNGFNLKQTLWLQWEEGNNNKKPGEAVVDLYVDDIEFF"
     gene            2417..>2897
                     /locus_tag="VFR02_04860"
     CDS             2417..>2897
                     /locus_tag="VFR02_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869808.1"
                     /translation="MKRTIRTTGWGLGLLALACLPQGCTQSVPGLGAVLAPPLPNTVV
                     SYFNYASTKVNPSLLGQLKGFFQDGTYGYTTDSGIQLEAPLFSAGLPDPSGSPYALHL
                     SGTYTDYGNAAYPAFELDCYPVANASYASGRMYDVSSFTGIKFSWNYPSDDNSQQRFF
                     "
BASE COUNT          535 a         1000 c          860 g          502 t
ORIGIN      
        1 gggtgtcggt caccctgggc gatttcgacg aggcctacac gcccctgacc ctctggaacc
       61 gggacaacct ggacctcaag tacatgcccg agatgatcgc ccgggaagac gacacgctga
      121 aatatgaaag cttcctcaat cacgagcccg agtggccctt ccggggcgcc cgggccggca
      181 cggccttgat gtggcccgac tcccagttgc tggaccgttt cagcgtcagc gccatgaccg
      241 acttcgtgag caacggcttc aacgacacct cgggccaggt cttttacttc ggcggcttct
      301 ccaacttcat cttcggcgcc aacgccgacc tgaagtcgcg ccgctggtac ctgggcgggc
      361 cttcgctcca gttggccatg gacgcctacg cggtcatcct ggcccagccg ctcggcacca
      421 acgcgccggg ttccatctac gtgcagttca atcccgatac ctgggcccac cattaccgga
      481 tcaccagcct gaagccctcg ttggatttcg gcctgggcga tgacgcctcc ttcggcggga
      541 cctgggaagg cgcggaggcc gtctaccagg acgatgagcg ggacaacaac aaggtcatca
      601 gcgactacgc catcctggcc gggccctact tccgtttcgg ccgctccaag atcagcttca
      661 attatctgca cgtcggcccc tattactatt cgcccctggc ccagacccgg caggacgacc
      721 tggtgcccgg gtcgggcgcg gacaacgccg tcacgccgga catcttcacg ccgccgctca
      781 ggagccagtt cttcctttcg gccgtgcccc gggcggggga tatcttcagt ttctacgacc
      841 gcacccagga caataccttc ccctatggcc tctccacccc caaccgggaa ggcggcggcc
      901 tggacttgga cgtgaaaacg ctcgaaaagg acgccctcaa gatcaagggc tcggtctact
      961 tcgtccagga gatcagtgat aacctggtgg tgaacgccgc cggcacgggg gtgacgacca
     1021 tcgacggcaa cccggccgcc cccacgcccc agcgcagctt cgcctacgtg aacctggggc
     1081 cctcgatcga cctggggccc ttgctgcaga tgtccaccga cctggtggtg ggggtgaacg
     1141 cccgttatga gcgcaccgac agctccctgg gcaccctgac cagcacctgg atcctgggcg
     1201 gggcgcaggt gggcatcctc ccctggtggg aatttgcggt atcctatggg gaccaggaga
     1261 tcagcgggca ggaggcgggt tacggcggca gcaccctggc ccggtacgcc tatctgttcg
     1321 acaacacgga cctgggccag taccaggtct tcaacatcaa cggcggcaac cagagtttcc
     1381 ggctgtccag cgtgttcaag atcagccgca actcgaccct ggccctggat tgggcccaga
     1441 ccggcgggaa cgcggtccct tacgtgggcg ccacgcccac gggcaacagc gtgaacaacc
     1501 agtacgcgga gatgacctat gagatccaat tctaaaggcg cttgggcggc cttcgcgctg
     1561 gcctcgtcct tcctggtgct ggcgggggcg ggctgccaga acgccttgcc gggtttcggc
     1621 gcctatcagg ccacgggcac cacgcccaac accaaccggg tggaccaatt cgattcagga
     1681 tcggtctcca tcaaccccaa gcttttcgag aacagcaatc cgcccaacta cgtcctgcgc
     1741 agccccgggg cggtgaccct ggtcaacaac ttcgcctcct acggctcctt ccatccggtc
     1801 ggcagcgacc ccaatgtggg gctggatccg ggcggctacg tcagcgcgaa ttgcttccgc
     1861 atgtcggggg cggtgacgga cacgctggac aacaccttcc cggcgctgga cctggaagtg
     1921 cccctggaga actataattt ttacgacgcc aaccatttca cgggcgtcaa gttctacatg
     1981 aaggtggcca acgacgacac ggccttccac cgggtcttcg cggtcccggt ggcccagacc
     2041 gtcccctcgg gcaccaacaa cggcacctgc ccggcgggcc ccaagcgctg ctacgaccac
     2101 ttcgcggccg actatagcgg gggcacgggc ggcaactgga tcctgtccag ctacaacttc
     2161 agcgacctga cccagctgca gtcgggctac gcgccccaac cttacgccac cctcaccggg
     2221 gggaacggct tcaacctcaa acagacgctc tggctgcaat gggaagaggg caacaacaac
     2281 aagaagccgg gcgaagccgt ggtcgacctt tacgtcgacg acatcgagtt cttctgaccc
     2341 ccagcggggg ggcccggccg gggcgcggga atggtggtac actttttagg tcgatggaca
     2401 gggcggagga caagggatga aacgaacgat ccgaacgacg ggatggggct tgggcctctt
     2461 ggccctggct tgcttgcccc agggttgcac ccagagcgtg cctgggctcg gggccgtgct
     2521 ggcgccgccc ctgcccaaca cggtggtctc ttatttcaac tacgcgtcga ccaaagtgaa
     2581 ccccagcctc ttgggccaat tgaagggctt ctttcaggac ggcacttacg gctataccac
     2641 cgacagcggg atccagctgg aagcgcccct cttcagcgcc ggcctccccg accccagcgg
     2701 cagcccctat gccctgcacc tgtccggcac ctatacggac tacgggaacg ccgcctaccc
     2761 ggcctttgag ctggactgtt acccggtggc caacgcctcc tacgccagcg gccggatgta
     2821 cgacgtctcc tccttcacgg gcatcaagtt cagctggaac tatccttcgg acgacaactc
     2881 gcagcagcgg ttcttct
//
LOCUS       DASTJJ010000390         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1155510, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000390 DASTJJ010000000
VERSION     DASTJJ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155510"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..955)
                     /locus_tag="VFR02_04865"
     CDS             complement(35..955)
                     /locus_tag="VFR02_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9869809.1"
                     /translation="MSEVLIVGSVAYDGLKTPYGSTPRTLGGAATYSSLASSLYAKTH
                     IVGVVGEDFRAQDLALLKSKKIGLDGLARAKGKTFFWKGFYKKEDMNVAYTLKTELNV
                     FGDFDPKIPEKDRKIPFLFLANIHPALQYKVTQQMRRPKFTLLDTMNLWINIARKDLL
                     KVMGKVDLMVLNDQEVRLLTGEAHLKKAAHAVLKLGPKRVIVKKGEHGAMLIGPEGTF
                     LAPAMPLDRVVDPTGAGDTFAGGFIGYLARRGQVTLESLKQAIVVGSLTASFTVQELG
                     VRSVASLTLAKVRARAKEFYRFASLPTVKL"
BASE COUNT          178 a          329 c          317 g          211 t
ORIGIN      
        1 ccctccgcgc ttgggttctt gatccttggt tggcctataa ttttacggta ggaaggctcg
       61 cgaatcgata gaattctttg gcccgcgccc gcaccttggc cagggtgagg gaggccacgc
      121 tgcgcacgcc cagctcctgc acggtgaagg aagccgtcag gctgcccacc acgatggcct
      181 gcttgaggct ctccagggtg acctggccgc gccgggccag gtagccgatg aagccgcccg
      241 cgaaggtgtc gccggccccg gtcgggtcca ccacccggtc cagcggcatg gcgggcgcca
      301 ggaaggtgcc ttccgggccg atcagcatgg cgccatgctc ccctttcttg acaatgaccc
      361 gcttgggccc cagcttcagc acggcatggg cggccttctt caggtgcgct tccccggtca
      421 ggagcctgac ctcctggtcg ttgagcacca tcaggtccac cttccccatg accttcaaaa
      481 gatccttgcg ggcgatgttg atccagaggt tcatggtgtc caacagggtg aatttcgggc
      541 ggcgcatctg ctgggtgacc ttgtactgca gcgccgggtg gatgttggcc aggaacagga
      601 aggggatctt gcggtccttt tccgggatct tggggtcgaa gtccccgaag acgttcaatt
      661 cggtcttcag ggtataggcc acgttcatgt cttccttctt atagaagccc ttccagaaga
      721 aggtcttgcc cttggcccgg gccaggccgt ccaggccgat cttcttggac ttgagcagcg
      781 ccaggtcctg agcgcggaag tcctccccca ccacacccac gatgtgggtc ttggcataga
      841 gcgaggaagc caggctcgaa taggtggccg ccccgcccag ggtgcgcggc gtggagccgt
      901 agggcgtctt caagccgtca taagccaccg acccgacaat gagcacttcg gacatttcga
      961 acacctttcc gatggatgga tttcggcccc gggatccccg gacccgcttc aacaacgacg
     1021 agtatacact tttcc
//
LOCUS       DASTJJ010000391         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1401053, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000391 DASTJJ010000000
VERSION     DASTJJ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401053"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFR02_04870"
     CDS             complement(<1..910)
                     /locus_tag="VFR02_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869810.1"
                     /translation="MSLTPSDPRLKAVIALHQSGRLAEAADGLLKLIGTHPENPGLWA
                     HLGSLRLQQGMAGEAREAFARAAALQPAFAPAHAGLGQALEKLGRRVEALVSYDRALA
                     LRPEAWIQFNRGNLLRDLGSLDEALESYGEALRLDPGLEEALTNRGALLLAARRKGEA
                     LEDFERALSLRPDRPEGLNNRGNALRALGRPLEALADYDRALSLRPDHADSWNNRGIA
                     LQELGRLPEALEAYDRAIALDPAFAFAYWNKSQAKLLTGDFEEGWRLFEWRWKSATKN
                     EARDLGRPPWLGDEALEGKTLLVWAEQ"
     gene            complement(997..1575)
                     /locus_tag="VFR02_04875"
     CDS             complement(997..1575)
                     /locus_tag="VFR02_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9869811.1"
                     /translation="MHKTRLEAFSDGVVAILITILVLDLKVPQGADLDSLRPVLPVFL
                     AYVLSFVYLGIYWNNHHHMLHAVGRVDGRVLWANLHLLFWLSLVPFVTAWMGENRFEP
                     VPTAAYGVVLLMAAFAYFILQSSLIRLEGKDSQLAQAVGRDGKAYFSLACYVAAILLA
                     FLAEWISWILFVVVALLWLVPDRRIESRFGGS"
     gene            1690..>1887
                     /locus_tag="VFR02_04880"
     CDS             1690..>1887
                     /locus_tag="VFR02_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET9869812.1"
                     /translation="MKPRVPGSTANDPRIYWAKERTFLAWLRTGLALMGFGFVVARFG
                     LFLRMVRLNEPLPAAAQPGWSM"
BASE COUNT          325 a          670 c          611 g          281 t
ORIGIN      
        1 cctgctcggc ccagaccaat agggtcttgc cttccaacgc ctcatcgccc aaccagggcg
       61 gccggcccag gtcccgggcc tcgttcttcg tcgcgctctt ccagcgccac tcaaaaagcc
      121 gccagccttc ctcaaagtcc cccgtcagca gcttggcttg ggacttgttc cagtaggcga
      181 aggcgaaggc cgggtccagg gcgatggccc ggtcataggc ttccagggcc tcgggcaggc
      241 ggcccaattc ctgcagggcg atgccccggt tgttccaact gtccgcgtgg tcgggccgca
      301 gcgacagggc ccggtcgtaa tcggccaggg cttccagggg ccggcccaag gcccgcaggg
      361 cgttgccccg gttgttgagg ccctccgggc ggtcgggccg aagggacagg gcgcgctcga
      421 aatcctccag ggcctccccc ttgcggcgcg ccgccaacaa gagggcgccg cggttggtca
      481 gggcctcctc caaccccggg tccagccgca gggcctcccc gtaactttcc agcgcctcgt
      541 ccaggcttcc caggtcccgg agcaggttcc cccggttgaa ctggatccag gcctcgggcc
      601 tcagggccag cgcccggtca tagctcacca gggcctccac ccggcgcccc agtttttcca
      661 gggcctggcc caggccggcg tgcgcggggg cgaaggcggg ttgcagcgcg gccgcccggg
      721 cgaaggcttc ccgggcctcg cccgccatgc cttgttggag ccgcaaggac cccaagtggg
      781 cccacaggcc cgggttttcc gggtgggtcc cgatcagctt caggaggccg tcggcggcct
      841 cggccaagcg gccggactgg tgcagggcga tcacggcctt cagccgcggg tcggatgggg
      901 tcaaggacaa gggtcacctg ccaggatcgg aaatcctgcc ccggccatcg gcaaaggagc
      961 cccgcccggc gtcccccaag atccctcacg gcgcattcag gaacccccga accgcgactc
     1021 gatccgccgg tcgggcacca accagaggag cgccaccacc acgaaaagga tccaggagat
     1081 ccattcggcc aagaaggcca ggaggatggc cgccacgtaa caggccaggc tgaaataggc
     1141 cttcccgtcc cggcccaccg cctgggccaa ttgggaatcc ttcccctcca accggatgag
     1201 gctggattgg aggatgaaat aagcgaaggc ggccatgagc agcaccaccc cataggcggc
     1261 cgtggggacc ggctcgaagc ggttctcccc catccaggcc gtcacgaaag gcaccaggga
     1321 aagccagaac aagaggtgca ggttggccca aagcacccgg ccgtccaccc ggccgacggc
     1381 atgcagcatg tggtggtggt tgttccaata aatgcccagg tagacgaagc tcagcacata
     1441 agccaggaaa acgggaagga ccggccgaag cgagtccagg tccgccccct ggggcacctt
     1501 gaggtccaag accaggatgg tgatgaggat ggcgacgacg ccgtcgctga aggcttccag
     1561 gcgggtcttg tgcatggggc tcgctcccgg ggattggcag gcgccccatc atcccagatg
     1621 gggcgcgggg gctcaagacg gctccgaaaa atccttttcc tttcgcccct ccccggccac
     1681 aatagggcca tgaaaccccg cgtccccggc tccaccgcca acgatccccg tatctactgg
     1741 gccaaggaac gcaccttttt ggcctggctg cgcaccggct tggccctcat gggtttcggc
     1801 ttcgtggtgg cgcgcttcgg cctcttcctg cgcatggtcc gcctcaatga acccctgccg
     1861 gccgcggccc aacccggatg gtccatg
//
LOCUS       DASTJJ010000392         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_301175, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000392 DASTJJ010000000
VERSION     DASTJJ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301175"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFR02_04885"
     CDS             <1..1159
                     /locus_tag="VFR02_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9869813.1"
                     /translation="AQRFSESVIRGMSRVAAAHQAVNLAQGFPDFPADERIKRAAQEA
                     IGRDINQYAVTWGAKRLRDAIVAKEKRFRGRELDPETQVTVCCGATEGMMAAFLAVID
                     PGDEAIIFQPFYENYGPDTILSGATPRYVTLYPPASDQEDWTFQEEELAAAFNDKTKV
                     IVINTPHNPTGKIFRKKELEFIAALCQKWDVLAVTDEIYEHILYDGETHACLASLPGM
                     ADRTITVNGASKTFSVTGWRVGYVLAPEKLTTAIRKVHDFLTVGAAAPLQEAIASAMA
                     LPDDFYRHLADEYQGKRDYLLKGLREAGFPAFKTWGAYYLIAQIPEAGFGGDYEYAVH
                     LAKTAGVATVPVSSFYLDRPSTGMIRFCFAKKVETLAKAVEKLKAYRGVKV"
     gene            1313..>1586
                     /locus_tag="VFR02_04890"
     CDS             1313..>1586
                     /locus_tag="VFR02_04890"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9869814.1"
                     /translation="MTFYQSIESIFVAAPPARAYQALTDWKARAAWRPGLSIVWDGPD
                     QAAVGQVVHFRVAGFPPSFFSYRVTGLEPGRRIFMEYQAPPLRGRAA"
BASE COUNT          282 a          547 c          480 g          277 t
ORIGIN      
        1 ggcccaacgt ttttccgagt cggtcatccg gggcatgtcc cgggtggccg ccgcccacca
       61 ggcggtcaac ctggcccagg gtttccccga ttttccagcg gatgagcgca tcaagcgggc
      121 cgcccaggag gccatcggcc gggacatcaa ccagtacgcg gtcacctggg gcgccaagcg
      181 cctgcgggac gccatcgtgg ccaaggaaaa gcgcttccgg ggccgggagc tggaccccga
      241 gacccaggtc acggtctgct gcggcgccac cgagggcatg atggccgcct tcctggccgt
      301 catcgacccc ggcgacgagg ccatcatctt ccagcccttc tacgagaact acggcccgga
      361 caccatcctg tcgggcgcca cgccccgcta cgtgaccctc tatccgcccg cttccgacca
      421 ggaggactgg accttccagg aagaggagct ggccgcggcc ttcaacgaca agaccaaggt
      481 catcgtcatc aatacgcccc acaaccccac gggcaagatc ttccggaaaa aggaattgga
      541 gttcatcgcc gccctctgcc agaagtggga cgtactggcc gtcaccgacg agatctacga
      601 gcacatcctc tacgacgggg aaacgcacgc ctgcctggcc tccctgcccg gcatggcgga
      661 ccgcacgatc acggtgaacg gggcctccaa gaccttctcg gtgacgggct ggcgggtggg
      721 ttacgtgttg gcgcccgaga aactcaccac ggccatccgc aaggtgcacg acttcctgac
      781 ggtgggcgcc gccgcgcccc tgcaggaggc catcgcctcg gccatggccc tgcccgacga
      841 tttctaccgc cacctggccg acgagtacca gggcaagcgc gactacctgt tgaagggcct
      901 gcgggaagcc gggttccccg ccttcaagac ctggggcgcc tattacctga tcgcccagat
      961 ccccgaggcg ggcttcggcg gcgactacga gtacgccgtg cacctggcca agacggcggg
     1021 cgtggccacc gtaccggtct catccttcta cctggaccgg ccctccaccg gcatgatccg
     1081 cttctgtttc gccaagaagg tggagaccct ggccaaggcc gtggaaaagc tcaaggccta
     1141 ccggggtgtg aaggtctgac ggcttgaacc acggatgaac agggatggac acagatattt
     1201 tgaacggcaa ttccaacccc actcatccag gggtcggagc atcacaaggt cggtcttttt
     1261 ttctcatctg tgttcatctg tgtgcatctg tggttccaag ggtttttaaa ccatgacttt
     1321 ttatcaatcc atcgaatcca ttttcgtcgc ggcgccgccc gcgcgggcct accaagccct
     1381 tacggactgg aaagcccgcg ccgcttggcg cccggggctc tccatcgtct gggacgggcc
     1441 cgatcaggcc gccgtggggc aggtcgtcca tttccgcgtg gccgggttcc caccctcttt
     1501 tttctcctac cgcgtcaccg gattggaacc gggccgcagg atcttcatgg aataccaggc
     1561 tcccccgctg cggggccggg cggcgg
//
LOCUS       DASTJJ010000393         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1767341, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000393 DASTJJ010000000
VERSION     DASTJJ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767341"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR02_04895"
     CDS             <1..258
                     /locus_tag="VFR02_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9869815.1"
                     /translation="YFGFEKVPLDFIHGADAAANAKILTDVLEGKPGPYRDAVVMNAA
                     VGFYVAGKCADIAGGRPLAEEAIDSKRALGALKKLETLSHG"
     gene            283..1068
                     /gene="trpC"
                     /locus_tag="VFR02_04900"
     CDS             283..1068
                     /gene="trpC"
                     /locus_tag="VFR02_04900"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9869816.1"
                     /translation="MSILQTIGATKRDEVARLKRERGLDSLRQGAASQAPPRDFFGAV
                     ARPGKLSLIGELKKASPSKGVLKADFDVRQLAPLYAKAGARALSVLTDVQYFQGDLSY
                     LKQAQAASGLPVLRKDFLIDEHQVYEAREAGADAILLIVAMLSGGQLKELQQLAGELG
                     MTVLVEVHDEAELDAALKASVKLLGINNRNLNDFKVDLQTSLRLMKGLPAGLPVVSES
                     GIFTREDARSLKEQGASAILVGEAFMTAPDLPAAVRALLPEGD"
     gene            1070..1693
                     /locus_tag="VFR02_04905"
     CDS             1070..1693
                     /locus_tag="VFR02_04905"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9869817.1"
                     /translation="MVRVKICGVTRPEDVRLIAREGGDAIGFQMSQGPRKISPERAKG
                     LVRSVPPFVTPVGVFVDEPLSRVKALVKYCGFQAVQLHGGEDEAYCAALSLPVIKAVR
                     MKDARSARAYGDFRVAAFLLDHYNKDKRGGTGGSFDWKWAPRAVAQLPAPVLLAGGLT
                     PENVARAIQTAGPFGVDVASGVESRPGLKDPRKVSRFIRRAKKAVRG"
     gene            1708..>1943
                     /locus_tag="VFR02_04910"
     CDS             1708..>1943
                     /locus_tag="VFR02_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869818.1"
                     /translation="MADQKSLIIKLAHIYYHTGSWDKAIVEYQKIIALDPHDFNVRLT
                     LGELFVKKGDLDRAYREFEAAAAGFLSEKNTKKAL"
BASE COUNT          350 a          612 c          667 g          314 t
ORIGIN      
        1 tactttgggt ttgaaaaagt gccgctggac ttcatccatg gagccgacgc ggcggccaac
       61 gccaagatcc tgacggacgt cctggaaggc aagccggggc cctaccggga cgcggtggtc
      121 atgaacgcgg cggtggggtt ctatgtggcg gggaagtgcg ccgacatcgc cggcggacgg
      181 cccctggccg aggaagccat cgattcgaag cgggccctgg gcgcgctgaa aaaactggag
      241 accctgtccc acggctgagg ccggggcgcg gaaagcgaga ccatgagcat ccttcaaacc
      301 atcggggcca ccaaacgcga cgaggtcgcc cgcctgaaac gggaaagggg cctggattcc
      361 ctgcgccaag gcgcggcgtc ccaggcgccc ccgcgggatt ttttcggggc ggtggcgcgg
      421 ccggggaaac tttccctcat tggggaactc aagaaggcca gcccctccaa gggggtcctc
      481 aaggccgatt ttgacgtgcg gcaactcgcc cccctctacg cgaaggcggg ggcgcgggcc
      541 ctctcggtat tgaccgatgt ccaatatttc cagggggacc tttcctacct gaaacaggcc
      601 caagcggcct ccggccttcc ggtgctgcgc aaggatttcc tcatcgacga gcaccaggtc
      661 tatgaggccc gggaggcggg ggcggacgcc atcctgctca tcgtggccat gctgtcgggc
      721 ggccaactga aggaactgca gcaattggcg ggggagctgg gcatgacggt gctggtggag
      781 gtgcacgacg aggcggaatt ggacgccgcc ttgaaggctt ccgtgaagct gttggggatc
      841 aacaaccgca acctcaatga tttcaaggtc gaccttcaga cctccctgcg cctgatgaag
      901 ggcctcccgg cggggctgcc cgtggtgagc gagagcggca tcttcacccg ggaggacgcc
      961 cggtccctga aggagcaggg cgcctcggcc atcctggtgg gcgaggcctt catgaccgcg
     1021 ccggaccttc cggcggcggt ccgggccctc ctgcccgagg gggactgaca tggtgcgggt
     1081 caagatctgc ggggtgaccc gccccgagga cgtgcgcctc atcgcacggg agggcgggga
     1141 cgccatcggt ttccagatgt cccagggccc gcggaagatc agccccgaac gcgccaaggg
     1201 gctggtgcgg tcggtgccgc cctttgtgac gccggtgggc gtcttcgtgg acgaacccct
     1261 gtcccgcgta aaagcgttgg ttaaatattg cggtttccag gccgtccaac tgcatggcgg
     1321 ggaggacgag gcctattgcg cggctttatc cctgcccgtg atcaaggccg tgcggatgaa
     1381 ggacgcccgg tcggcccggg cctacgggga cttccgcgtt gcggcctttt tgttggacca
     1441 ctataataag gacaaaaggg gtgggaccgg cggatccttc gactggaagt gggccccccg
     1501 ggccgtcgcc cagctcccgg cccccgtgct gctcgccggc ggactgacgc cggagaacgt
     1561 ggcgcgcgcc atccagaccg ccgggccttt tggagtggac gtggccagcg gggtggaaag
     1621 ccggcccggc ctgaaagacc cccgaaaagt ctcccgcttc atccgccgcg ccaaaaaagc
     1681 cgtccgaggc tgaaaggacc ggtcctcttg gccgaccaga aatccctcat catcaagctc
     1741 gcccatatct actaccacac cggcagttgg gataaggcca ttgtggagta ccagaagatc
     1801 atcgccttgg acccccacga cttcaacgtg cggttgacct tgggggagct cttcgtgaag
     1861 aagggcgacc tggaccgggc ctaccgggaa ttcgaggccg ccgccgccgg gttcctgtcc
     1921 gagaagaaca ccaagaaggc cct
//
LOCUS       DASTJJ010000394         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_178613, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000394 DASTJJ010000000
VERSION     DASTJJ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178613"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..660
                     /locus_tag="VFR02_04915"
     CDS             202..660
                     /locus_tag="VFR02_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014949823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9869819.1"
                     /translation="MTPQSQLIPILMADDDADDKMLAAKALKENRVANPLVTVSDGEE
                     LLQYLHRSGPYRDAARPCFILLDLNMPRLDGREALKIIKGDPELRKIPVVILTTSRAE
                     EDIVRGYNLGANSYITKPVTFEGLVGVMSTLKTYWLEIVELPDGDPLANP"
     gene            635..>2210
                     /locus_tag="VFR02_04920"
     CDS             635..>2210
                     /locus_tag="VFR02_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9869820.1"
                     /translation="MATPSQTPKPLRLLMVDDDREDFESVRHLLAGLKEPGYVLEWAP
                     DYEAGLARVREGGLDLILVDYLLGPRSGLDFLRDASLLPDGPPLLVLAGQGSPELNRE
                     AIARGAADYLLKGGLTADQLHRSIRYALERAKVMRELRQRGEWFRALIENALDGVSIV
                     EKDGTTSYLSPAIEPMLGYTPQERAGRNSFELIHPEDAPRVAQALADLFEGPGRQQRV
                     EFRFKHKDGSWRFLEASAMSHDFQSPSLRGVVVHYRDISDRKSAEEVLRRSEGNLRAI
                     FNNSSQFICLMDKDANLVAFNEKASLNSVFLGGKPFEPGRNLMDYVPAPHHDMVRGRL
                     GEALAGRSLRLESQFTDPTGETHWYEVAYNPVQDEKGEVTGVCLMGSTIDERKRAEKA
                     LRESEERFRRVFENSPVGICLVGMDSKIQVANTRFCAIVGYPARELEGKSFIDITYPP
                     DIASDVQQYQQLVSGRIDSYEMEKRYIRKDRALVWANLTVTLVRDEAGRPLCGLGMAE
                     DVTERKKNEGALSELAA"
BASE COUNT          406 a          730 c          753 g          321 t
ORIGIN      
        1 ctggccatct gccgcaaggt ggtggagcgg cacggggggc gcatccgggc cgagggcggg
       61 gagggggagg gcgcggcctt ccacatcgac ctgcccctgc gccacgccga aggggaaccc
      121 caggaggccc gggcctgagg cccgggggca tcgttcgcac ccggcgcccg cgccggcaac
      181 tcaagatcga aaggaaacgc catgacgccc caatcccagc tcatccccat cctgatggcc
      241 gacgatgacg ccgacgacaa gatgttggcc gccaaggccc tgaaggagaa ccgggtggcc
      301 aaccccctgg tgaccgtgtc cgacggcgag gagctgttgc agtacctgca ccgatccggg
      361 ccctaccggg acgcggccag gccttgcttc atcctgttgg acctcaacat gccccggctg
      421 gacggacggg aagccctcaa gatcatcaag ggcgacccgg agcttcgcaa gatccccgtg
      481 gtcatcctga ccacgtcccg ggccgaggag gacatcgtcc ggggctacaa cctgggggcc
      541 aactcctaca tcaccaaacc ggtgaccttc gaggggttgg tgggggtgat gagcaccttg
      601 aagacctatt ggctggagat cgtggagctg cccgatggcg accccctcgc aaacccctaa
      661 gcccctgcgg ctgctcatgg tcgacgacga ccgggaggac tttgagtccg tccggcacct
      721 gctcgccgga ttgaaggagc cgggctatgt cctggaatgg gcccccgact acgaggcggg
      781 cttggcccgg gtccgcgagg gcggactgga cctgatcctg gtggattacc tgctggggcc
      841 ccggtcgggg ctggatttcc tgcgggacgc gtcgctcttg cccgacgggc cgccgctcct
      901 ggtgttggcc ggacagggca gcccggaact caaccgggag gccatcgccc ggggggccgc
      961 cgattacctg ctgaaggggg ggctgaccgc ggaccaactg caccgctcca tccgctacgc
     1021 cctggaacgc gccaaggtca tgcgggaact gcggcaaagg ggcgaatggt tccgggcgct
     1081 catcgagaac gccctggacg gggtcagcat cgtggaaaag gacggcacca cctcctacct
     1141 gagcccggcc atcgagccca tgctgggcta taccccccag gaacgcgcgg gccgcaacag
     1201 cttcgagctc atccatcccg aggacgcccc ccgggtagcc caggccctgg ccgacctctt
     1261 cgaggggccg ggccgccagc aacgggtgga attccgcttc aagcacaagg acgggtcctg
     1321 gcgcttcctg gaggcctcgg ccatgagcca cgattttcag agcccctcgc tgcggggggt
     1381 ggtggtgcat taccgggaca tctcggaccg caagtcggcc gaggaggtcc tgcgccggtc
     1441 cgagggcaac ctgcgggcca tcttcaacaa cagctcccag ttcatctgcc tgatggacaa
     1501 ggacgccaac ctggtggcct tcaacgagaa ggccagcctc aactccgtct tcttgggcgg
     1561 caaacccttc gaaccgggcc gcaacctgat ggactatgtg cccgcgccgc accacgacat
     1621 ggtgcgggga cggctggggg aggccctggc gggccgctcc ctgcgcctgg aaagccagtt
     1681 caccgacccg acgggggaga cccattggta tgaggtcgcc tacaacccgg tccaggacga
     1741 aaagggggaa gtcacgggcg tctgcctgat gggatcgacc atcgacgaac gcaagcgcgc
     1801 cgaaaaggcc ctccgggaaa gcgaggaacg gttccgccgg gtcttcgaga acagcccggt
     1861 gggcatctgc ctggtgggga tggactccaa gatccaggtg gccaacaccc ggttctgcgc
     1921 catcgtgggg tatccggccc gggagctgga aggcaagtcc ttcatcgaca tcacctatcc
     1981 gccggacatc gcgtcggacg tccagcaata ccagcagctg gtgtcgggcc gcatcgacag
     2041 ttatgagatg gaaaaacgct acatccgcaa ggaccgggcc ctggtctggg ccaacctgac
     2101 cgtgacgctg gtgcgcgacg aggccggccg gccgttgtgc ggcctgggca tggccgagga
     2161 cgtgaccgaa cgcaagaaga acgagggggc gctgtccgaa ttggccgcca
//
LOCUS       DASTJJ010000395         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_55963, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000395 DASTJJ010000000
VERSION     DASTJJ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55963"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1235
                     /locus_tag="VFR02_04925"
     CDS             <1..>1235
                     /locus_tag="VFR02_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869821.1"
                     /translation="HVVNEGSAPATALGLRFKVVPAWNVQARFHADGGDLLVTQPPTK
                     AKRRTYWRLAAFDPRGASAGGKALETELPALPPGGEAWTAYFLGSSDSPEEGARLAAR
                     LRSQGAAPLLDDTRDYYQKWFAQGAEFSGDPKVADLFEIQSLIFKCEESHSGGFSPLV
                     GYSYTWIRDNNGPIRWFLKTGHVAEAKGAIDFFHGVAASMGSLPNSIRVDYPLTYRLK
                     DLSGIHVEHAETPNWIVLQHWWYYLATGDLDTIRQRWAYLKRCLTGQVQEDGKFFFHR
                     DETYLWCLESRCFDHESYPNYDLSTYAFAVDSSFDFVAAADRLAALGGRLGWKVGDLQ
                     QMSDAARRTAEQTYWNAKDRYWAPAQSLLGPLYNAPYANILLNPFWCGYARNDLDPAG
                     ETPEAETEAKQALTAGYQW"
BASE COUNT          203 a          435 c          403 g          194 t
ORIGIN      
        1 gcacgtggtc aacgagggga gcgcccccgc gacggccttg gggctgcggt tcaaggtggt
       61 cccggcctgg aacgtgcagg cgcgcttcca cgccgacggc ggcgacctgc tcgtcaccca
      121 accgcccacc aaggccaaac ggcgcaccta ttggcgcctg gcggctttcg acccccgggg
      181 cgcctcggcc gggggcaagg cgctggagac cgaattgccg gccctgccgc ccgggggaga
      241 ggcctggacg gcctatttcc tcggctcgtc ggacagcccg gaggaggggg cccgcttggc
      301 cgcccggttg cgctcccagg gcgcggcgcc cctcttggac gacacgcggg actactacca
      361 gaaatggttc gcccaagggg ccgaattctc gggcgacccc aaggtcgccg acctcttcga
      421 gatccagtcc ctcatcttca aatgcgagga gtcccactcg ggcggcttct cgcccctggt
      481 gggttattcc tacacctgga tccgcgacaa caacgggccc atccggtggt tcctgaagac
      541 ggggcacgtg gccgaggcca agggcgccat cgacttcttc cacggcgtgg cggccagcat
      601 ggggtcgctg cccaacagca tccgggtgga ttatcccctg acctaccgtt tgaaggacct
      661 ttcgggcatc cacgtggagc acgccgagac gcccaattgg atcgtcctgc agcactggtg
      721 gtattacctg gccacggggg acctggacac catccggcag cgatgggcct acctcaagcg
      781 ctgcctgacc ggccaggtgc aggaggacgg caaattcttc ttccaccggg acgagaccta
      841 tctctggtgc ctggagtcgc gctgcttcga ccacgagtcc tatcccaact acgacctgtc
      901 cacctacgcc ttcgcggtgg attccagttt cgacttcgtg gccgcggccg accggctggc
      961 ggccctgggc ggccgcctgg gctggaaggt gggggacctc cagcagatgt ccgacgcggc
     1021 ccggcggacc gccgaacaga cctattggaa cgccaaggac cgatactggg ccccggccca
     1081 atcgttgctg ggccccctgt acaacgcgcc ctacgccaac atcctgctca accccttttg
     1141 gtgcggctac gcccgcaacg atctggaccc ggcgggggag acccccgagg cggagaccga
     1201 ggccaagcag gccctgacgg cgggatacca atggc
//
LOCUS       DASTJJ010000396         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_423130, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000396 DASTJJ010000000
VERSION     DASTJJ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423130"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VFR02_04930"
     CDS             <1..132
                     /locus_tag="VFR02_04930"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309309.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9869822.1"
                     /translation="EFVLENQANRPVYNSVLRIDSEHLIERVGKQVRSMFSWLKDKK"
     gene            282..929
                     /locus_tag="VFR02_04935"
     CDS             282..929
                     /locus_tag="VFR02_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET9869823.1"
                     /translation="MVVPTAVVAAVFFVLAAYTRQWPWAVPGAAAAVLALLMLNFFRD
                     FERRPVAEPGPRAVLSPADGKVVVLKKAVEKFYMKKPMLHVAIFMNPLNNHVQRAPFD
                     GKVVKKAYHAGEFLAAYDDKADLVNEQAHLVLELDKKKGMKAKHRVVLKQIAGLLCRR
                     VKADAKPGQRVVRGERFGRILLGSRVDLFLPVGFKPSVKKGDQVRAGLTVLGELP"
     gene            926..>1270
                     /locus_tag="VFR02_04940"
     CDS             926..>1270
                     /locus_tag="VFR02_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9869824.1"
                     /translation="MSPARPKASGKSPANSRLRKSIYLLPNILTAANMVFGILSITYS
                     IQDSLTLMAADNNSILPFVLPAKLILVSIFLDFMDGRVARATGTTSRFGMEFDSLSDL
                     ISFGMAPAILIYL"
BASE COUNT          232 a          413 c          391 g          234 t
ORIGIN      
        1 gagttcgtgc tggagaacca ggccaaccgc ccggtgtaca actcggtgct gcggatcgat
       61 tccgagcacc tgatcgagcg cgtgggcaag caagtgcgca gcatgttcag ctggctgaag
      121 gacaagaagt aaaatttttc accaccaagg caccaagttc accaagaaag gctttagatc
      181 ctaagctgaa gacccttccg ttcttggtgc cttagtgtct tggtggtgaa tacgccttgg
      241 agtttattat gcggctgccg ttcatgaagg aaacctgggg gatggtcgtt cccaccgccg
      301 tcgtggcggc ggttttcttc gtcctggcgg cctacacccg gcagtggccc tgggcggtgc
      361 cgggggccgc ggcggccgtc ctggccctgc tgatgctgaa ctttttccgc gatttcgagc
      421 gcagacccgt ggccgagccg ggcccccggg ccgtcctcag cccggcggac ggcaaggtcg
      481 tggtgctgaa gaaggccgtg gagaagttct atatgaagaa acccatgctg cacgtggcca
      541 tcttcatgaa ccccctgaac aaccacgtcc agcgggcgcc cttcgacggc aaggtggtga
      601 agaaggccta ccacgccggc gagttcctgg ccgcctatga cgacaaggcg gacctggtca
      661 acgagcaggc ccacctggtc ttggaactgg acaaaaagaa gggcatgaag gccaagcacc
      721 gcgtggtcct gaagcagatc gcggggcttc tgtgccgccg ggtcaaggcc gacgccaagc
      781 cgggccagcg ggtcgtccgg ggcgagcgct tcggccgcat cctgctgggc tcgcgggtgg
      841 acctgttcct gccggtgggc ttcaaaccct cggtcaagaa gggcgaccag gtacgggccg
      901 gcctgaccgt cctgggggaa ctgccatgag ccccgcccgc cccaaggcgt ccgggaagtc
      961 cccggccaac tcgcgcctgc gcaaatccat ctacctgctg cccaacatcc tgacggccgc
     1021 caacatggtc ttcggcatcc tgtccatcac ctattcgatc caggactcgc tgacgctcat
     1081 ggccgccgac aacaattcca tcctgccctt cgtgctgccc gccaagctca tcctggtgtc
     1141 catcttcctg gacttcatgg acggccgggt ggcccgcgcc acgggcacca ccagcaggtt
     1201 cggcatggaa ttcgacagcc tgtccgacct gatcagcttc ggcatggcgc cggccatcct
     1261 catctacctg
//
LOCUS       DASTJJ010000397         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1524458, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000397 DASTJJ010000000
VERSION     DASTJJ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524458"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..960
                     /locus_tag="VFR02_04945"
     CDS             163..960
                     /locus_tag="VFR02_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869825.1"
                     /translation="MAAIFMVLSTTLITTGVKLVSNAAREAKGVQTNVAEADNAARAG
                     LEDALDYFIRNNGQMHAYTQSVQPGGTGVFQSGISYVDQPFSPAYNTNPKQSDTIDQT
                     GSIGLVSEYGLDTQVLPSANLIARYEVRKQGPGAYDPLAVHDISGQRAVSEVNGDGAA
                     WSVVSTGYVYKRLDHTYAGTYQQGVCPQWNTDYNQSPNQLVTKVTVSTEFRQVSLNLP
                     TVDATTAVGAVYCDAVTQVHMSNSSTKISGAVTAGTNFAVLGMTHDH"
     gene            950..2143
                     /locus_tag="VFR02_04950"
     CDS             950..2143
                     /locus_tag="VFR02_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869826.1"
                     /translation="MTTKKRSYIIPLFALVFAFLTTGMAPNPSRSAPSITSYSVHSSL
                     FCTDYSTTMAVTVNLSVGGGFSWNYPVVAEVALGFANQNNINGSVAWASGAPLGTTYT
                     TGTNCAGLSTDVSVTFTSASSKATVVNIKTPPNTGIVYNYVIMACRLSSAPTCGSFND
                     SDSVLIPCGAATPTFTPTLSPTVTLTPTITPTRTPTPTSTWTPTRTFTPLICPTPIGA
                     TSSTRYGGCTCFQNQSTLLSDTQVFGMSLANLQAVADYSGTEASPASILSPWKLTYYS
                     GSVTFDTGATGPLASFVAQGLLVVDGNLDMEPGCCSFDGVIFCTGNVTIKDGDTVLGA
                     IILGTPYYNGGGSTRSVLTISGSGGNYGQIIYSPVFVNEVQQKVAVYKEDFSQRKTYQ
                     TVPNW"
     gene            2150..>2870
                     /locus_tag="VFR02_04955"
     CDS             2150..>2870
                     /locus_tag="VFR02_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9869827.1"
                     /translation="MRSLKRLGQRGVSLVELVIAVAIMATMASSVILARGLMAKQTVR
                     TDDRAFATQKAIQMFGELKSLVSGSETGVQILDNYSDGTYYNTVLTTDKNVDTSPPGS
                     GNAANVLSGNRKSNGNWRFLRQISVVPLVNDTSARLVSIKVWLYAGDQNPGQPGELLS
                     EVSGIMRTSADLNNPTQVLDVYFLAINNIQGWWSQVPNLASNIKQAINDVELTSNKTL
                     QIRPHYITRTSYGQDLQYLPFI"
BASE COUNT          587 a          981 c          782 g          520 t
ORIGIN      
        1 aaaacttcca ttttcgattc tgagggcggc aaaatgtcga gacgttccaa tccttcgatc
       61 aaacctagat ccagtccggg cccgtccggg tcccggtccg gaattccgcg ccgttccccc
      121 gcccccttga agcagccggg acaaagggga aatgccctgg tcatggcggc catcttcatg
      181 gtgctttcca ccacgctcat caccacgggc gtcaagctgg tgtccaacgc ggcccgggaa
      241 gccaaaggcg tccagaccaa cgtggccgaa gccgacaacg cggcccgcgc cggcctggag
      301 gacgccctcg attatttcat ccgcaacaac ggccagatgc acgcctatac ccagagcgtc
      361 caaccgggcg gcacgggggt cttccaatcc gggatcagct acgtggacca acccttcagc
      421 cccgcctaca acaccaatcc caaacagtcc gacaccatcg accagaccgg cagcatcgga
      481 ctggtcagcg aatacggcct ggacacccaa gtgctgccca gcgccaattt gatcgcccgt
      541 tacgaggtcc gcaaacaggg gccgggggct tatgaccccc tggcggtgca cgacatctcg
      601 gggcaacgcg ccgtgagcga agtgaacggc gatggggcgg cctggagcgt ggtcagtacg
      661 ggttacgtct ataagcgctt ggaccatacc tacgcgggca cttaccagca aggcgtctgc
      721 ccccagtgga acacggacta taaccaatcc cccaatcaac ttgtcaccaa ggtgaccgtc
      781 tccaccgagt tccgccaagt gagcctcaac ctgcccaccg tggacgccac caccgccgtg
      841 ggggccgtct attgtgatgc cgtcacccag gtccatatgt ccaacagctc caccaagatc
      901 tcgggtgccg tcaccgcggg cacgaatttc gccgtcctgg ggatgaccca tgaccactaa
      961 aaaacgaagc tacatcatcc ccctattcgc gctggtcttc gccttcctga ccaccggtat
     1021 ggcccccaac cccagccgca gcgccccgag catcacgagc tattcggtcc attcctcgct
     1081 cttctgcacc gactactcga ccacgatggc ggtcaccgtg aatctctccg tcggcggcgg
     1141 cttctcgtgg aactatccgg tggtcgccga ggtggctttg ggtttcgcca accaaaacaa
     1201 catcaacgga tccgtggctt gggccagcgg ggcacccctg gggaccactt acaccaccgg
     1261 gacgaactgc gcggggttga gcaccgacgt cagtgtgacc ttcacttcgg cttccagcaa
     1321 ggccacggtc gtcaacatca agacgccccc caataccggg atcgtctata attacgtcat
     1381 catggcctgc cgcttgagct cggcgcctac ctgcggcagt ttcaacgact cagattcggt
     1441 gttgatccct tgcggcgcag ccacgccgac tttcacgccc acgctcagtc cgaccgttac
     1501 gttgaccccc acgatcacgc cgacccgcac ccccacgccc acatccacct ggacgccgac
     1561 ccggaccttt acgcccctca tttgtcccac gcccatcggc gccaccagca gcacccgtta
     1621 tggggggtgc acctgtttcc aaaaccagag cacgttgctt tcggataccc aggttttcgg
     1681 catgagcctg gccaacctcc aggcggtggc ggactatagc ggaaccgagg ccagcccggc
     1741 cagcatccta tcgccttgga agctcacgta ttattcggga agcgtgacct ttgacaccgg
     1801 cgccacgggg cccctcgcct cgtttgttgc tcagggactt ttggtggtcg acggcaactt
     1861 ggacatggag ccgggttgct gctcctttga cggtgtcatt ttctgcacgg ggaatgtcac
     1921 catcaaggat ggggacacgg tgctgggggc cattattctt ggcaccccct attacaatgg
     1981 cggaggcagc acccgcagcg tcctgaccat ttcggggtcg gggggcaact acggccagat
     2041 catctattcg cccgttttcg tgaatgaagt ccaacagaaa gtggcggtgt ataaggagga
     2101 cttctcccag cggaagacct accaaaccgt ccccaactgg taggccgcca tgcgatccct
     2161 caaacggctc ggccagcgcg gcgtcagctt ggtggaactg gtcatcgccg tggccatcat
     2221 ggccaccatg gcttcctcgg tcatcctggc ccggggtttg atggccaagc agacggtccg
     2281 caccgatgac cgggccttcg ccacccagaa ggccatccag atgttcggtg agctcaagtc
     2341 cctggtcagc ggttcggaaa cgggcgtgca gatcctggac aactactcgg acggcaccta
     2401 ctacaacacg gtcctcacca ccgacaagaa cgtcgacacc agcccgccgg gaagcggcaa
     2461 cgccgccaat gtcttgagcg gcaaccgaaa gtccaacggc aactggcgct tcctgcggca
     2521 gatctcggtg gttcccctgg tgaacgacac ctcggcccgg ttggtctcca tcaaggtctg
     2581 gctttatgcc ggggaccaga accccggaca gccgggggag cttttgagcg aggtcagcgg
     2641 catcatgcgc accagcgcgg acctgaacaa ccccacccag gtgctggacg tctatttcct
     2701 ggccatcaac aacatccagg gatggtggtc ccaggtgccg aacctggcca gcaacatcaa
     2761 gcaggccatc aatgacgtgg aattgacatc gaacaagacc ctgcagatcc ggccccatta
     2821 catcacccgg acctcttatg gccaggacct tcagtacctg cccttcatca
//
LOCUS       DASTJJ010000398         1444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_667681, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000398 DASTJJ010000000
VERSION     DASTJJ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667681"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFR02_04960"
     CDS             <1..208
                     /locus_tag="VFR02_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869828.1"
                     /translation="TGDAKRMGKSAGSDRERGKMTYPAAVGLDASKREVAKLTREALA
                     LLKPFGRKAEPLAELARHMAERTV"
     gene            249..665
                     /locus_tag="VFR02_04965"
     CDS             249..665
                     /locus_tag="VFR02_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET9869829.1"
                     /translation="MLLVMMVAWFLAQTWKMLNYYHKNHKINFRLLVGTGGMPSSHSA
                     FVCSLAASLGFEQGWDSSLFYLGLGVALIVMTDAAGVRRAAGQQAQILNEMMDDLYKS
                     RPIPPTKLKELLGHTPTQVVVGAFLGIAVAVLFYTL"
     gene            850..>1444
                     /gene="thiL"
                     /locus_tag="VFR02_04970"
     CDS             850..>1444
                     /gene="thiL"
                     /locus_tag="VFR02_04970"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET9869830.1"
                     /translation="MTDKKQTIGSLGEFGFIEKIRDQVRSKSPSVVLGIGDDAAILKP
                     SPGHEMVFTTDMLVEGRHFDLKTITPWQLGAKSMAVNVSDCAAMGAKPTAAVVSLGVP
                     GHFEVASLEAFYDGLKGWGAQFGVSVVGGDTVASDALVVNVALIGEVETGKALRRSGA
                     KAGDAIFVTGTLGDSAAGLEALLHPSPKGKESEPLLVK"
BASE COUNT          270 a          429 c          469 g          276 t
ORIGIN      
        1 gaccggtgac gccaagcgga tgggaaagtc cgccggaagc gaccgcgaaa ggggtaaaat
       61 gacctatccc gccgccgtgg ggttggacgc gtcgaaaagg gaagtggcga aattgacccg
      121 ggaagccctg gcgctcttga agcccttcgg ccggaaggcc gaacccctgg ccgagctggc
      181 ccgccacatg gccgaaagga cggtctgagt tggagccctt tttccaccac caccaccacc
      241 cctccaaagt gctccttgtc atgatggttg cgtggttttt ggcccagacc tggaagatgt
      301 tgaattacta ccataagaat cataaaatca actttcgcct tctggtgggc acggggggga
      361 tgcccagctc gcattccgcc ttcgtctgct ccctggcggc ttccttgggg ttcgagcagg
      421 ggtgggattc gtccctcttc tacctcggcc tgggggtggc cctgatcgtg atgaccgacg
      481 ccgcgggcgt gaggcgcgcc gccggccagc aggcccagat cctcaacgag atgatggacg
      541 acctttacaa atcccgtccc atccccccga ccaagctcaa ggaactgctg gggcatacgc
      601 cgacccaggt ggtggtgggg gccttcttgg ggatcgccgt ggccgttttg ttttatacgc
      661 tttaggataa gccgctttca ccacgagccc ctgcggggcc acgaagtgca cgaagaaaaa
      721 cttgatgccc gaaaacttca tgatcttttc gatcggatcc aaggattttt ttacttagtg
      781 gacttagtga cccccgcagg gggtttgtgg tgaaatgttt cctgttttta aatccttaaa
      841 agggaaaaaa tgacggataa aaagcagacc atcggatcgc tgggggaatt cgggttcatc
      901 gaaaagatcc gcgaccaggt gcggtccaag agcccctcgg tggtgctggg catcggcgac
      961 gacgccgcca tcctcaagcc ctcgccgggc catgagatgg tcttcaccac cgacatgctg
     1021 gtggagggcc gccacttcga cctcaagacc atcacgccct ggcagttggg cgccaagagc
     1081 atggccgtca acgtcagcga ctgcgcggcc atgggggcca agcccaccgc ggcggtggtg
     1141 tccttgggcg tgcccgggca tttcgaggtg gcgtccctgg aggccttcta cgacgggctc
     1201 aagggctggg gcgcccagtt cggggtctcc gtggtggggg gcgacaccgt ggcttccgac
     1261 gcgctggtgg tgaacgtggc cctgatcggc gaggtggaaa ccggcaaggc gctgcggcgc
     1321 tccggcgcca aggcgggcga cgccatcttc gtgaccggca ccctgggtga ttcggcggcg
     1381 ggcctggagg ccctgctgca cccgtccccc aaggggaagg aatccgagcc cctgttggtg
     1441 aagc
//
LOCUS       DASTJJ010000399         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_913209, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000399 DASTJJ010000000
VERSION     DASTJJ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913209"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFR02_04975"
     CDS             complement(<1..383)
                     /locus_tag="VFR02_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9869831.1"
                     /translation="MYLKKLEIHGFKSFADKTELAFEPGITAVVGPNGCGKTNVSDSV
                     RWVLGEQSAKGLRASEMTDVIFNGTDSRKPASLAEVTLTLSNEDKALALEYSEISITR
                     RVYRSGEGEYLINKVPARLKDVREL"
     gene            complement(551..>1330)
                     /locus_tag="VFR02_04980"
     CDS             complement(551..>1330)
                     /locus_tag="VFR02_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9869832.1"
                     /translation="PHQAWLSACLADAEKVFKRYGGRLAAIHLEPLIEGAGGMLTHPW
                     GYLKGLRRLCDRYDVLMILDEVATGFGRTGTMFACEQEGVLPDILCLAKNLTGGYLPL
                     AATMTTERVYRAFLGKFESGRTFYHGHTFTGNQLACAAALANLSLMERPAFLRGVQEK
                     CALLEELLAPLEPHPHVGDIRLRGLMGGIELVKDKRTGEGYPYARKMGYRVCALAKKQ
                     GVLLRPLGNVIVLMPPLGISKADLTRLVRVIALSIDRATRA"
BASE COUNT          249 a          415 c          419 g          247 t
ORIGIN      
        1 aagagctcgc gcacgtcctt caggcgggcc ggcaccttgt tgatgaggta ctcgccctcg
       61 cccgagcggt agacccggcg cgtgatggag atctcgctgt actccagggc caaggccttg
      121 tcctcgttgc tgagggtcag ggtgacctcg gccaggctgg cgggcttgcg gctgtccgtg
      181 ccgttgaaga tgacgtcggt catttcggag gcgcgcaggc ctttggccga ctgttccccc
      241 agcacccagc gcacgctgtc ggacacgttg gtcttgccgc agccgttggg gcccaccacg
      301 gcggtgatgc cgggctcaaa ggccagttcg gtcttgtccg cgaaggattt aaagccgtgg
      361 atctcgagtt tcttgaggta caagtggact ccgggtcggt ttggaatgga tagcctggcc
      421 gggcgaggag gaggagtggg aaaggtgagg atggcgccag aacttgggaa aatctagcat
      481 ccccaccggt agatgtcaaa atctttctaa accaccaaat atagggggaa tcagcctgga
      541 tcccccgctt ttaggcccgg gtcgcccggt cgatggaaag ggcgatcacc cgcaccaacc
      601 gggtgagatc ggccttcgaa atacccaaag gcggcatgag cacgatcacg ttccccaggg
      661 gcctcaggag cactccctgc ttcttggcca gggcacagac ccggtagccc atcttccggg
      721 cgtagggata gccttccccc gtccgcttat ccttcaccaa ttcaatgccg cccatcaagc
      781 ccctcaggcg gatgtcgccc acgtggggat gcggttccaa gggcgccaac agttcttcca
      841 gaagggcgca tttttcctgg acgccccgca ggaaggccgg cctttccatc aaggaaaggt
      901 tcgccagggc cgccgcgcag gccaattggt tccccgtaaa ggtatggccg tggtaaaagg
      961 tccggcccga ttcgaatttt cccaggaagg cccggtagac cctctcggtg gtcatcgtcg
     1021 ccgcaagcgg caggtagccc ccggtcaggt tcttggccag gcagaggatg tccggcaaga
     1081 cgccttcctg ttcgcaggcg aacatcgtgc ccgtgcgtcc gaagcccgtg gccacctcgt
     1141 ccaggatcat caggacgtcg taacggtcgc aaagccgccg aaggcccttc aaataacccc
     1201 aagggtgcgt cagcatgccg cccgcccctt cgatgagggg ctccagatgg atggccgcca
     1261 atcgcccccc atatcgcttg aagacctttt cggcgtcggc cagacaggcc gaaagccagg
     1321 cctggtgggg
//
LOCUS       DASTJJ010000400         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1768026, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000400 DASTJJ010000000
VERSION     DASTJJ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768026"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1178)
                     /locus_tag="VFR02_04985"
     CDS             complement(<1..>1178)
                     /locus_tag="VFR02_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869833.1"
                     /translation="GSTANPPSQAPASTAGGAQSSEGQTTPGGSAVQATFLDQIDIRF
                     SETIRGKASADQVQQSMAALASAFQQMVGFLLSNPGATADQVQGQIVAATQGTVSPDA
                     AKALVAAVQGFLQSLPPSQKALLSDESTRKNLFNQLFQGLGQAVAGAQPAALGGSTTE
                     TFDFQMAFGTQSVTSAGAGTQGSQFFEAGLSMAGFNDQNLRTSFTNSVLDGGPLTGAS
                     LADLSQQENVLLTNLASQSLASSTTATPGQTNGMGRALADLLGRAGAGRVDLASFTQA
                     QPTTSADQLNRVAPVDFNRVFALLTDSLRQASAIPPVTPSDTLQAPSPAMLPAFGTLP
                     PSAPETSILAAPAVVTLQNPTPQTLSTADPTDRILAVRQFFEQLSVSFNSVTAASTPA
                     "
BASE COUNT          191 a          349 c          448 g          190 t
ORIGIN      
        1 tgggcggggg tgctggccgc cgtcactgaa ttgaaagaga ccgaaagttg ctcgaagaac
       61 tggcgcacgg ccaggatccg gtccgtcgga tcggcggtcg acagggtttg gggcgtcgga
      121 ttctggaggg tcaccaccgc cggggccgcc aggatgctgg tttccggggc ggaggggggc
      181 aaggtcccga aagccgggag catggccggg gaaggcgcct gcagggtatc cgagggcgtc
      241 accggcggga tcgccgaggc ttgccgcagg ctgtcggtca acagggcgaa gacccggttg
      301 aaatccaccg gcgcgacgcg gttcaactgg tccgcggaag tcgttggctg ggcctgggtg
      361 aaggaagcca ggtcgacccg tcccgccccc gcccgtccca agaggtcggc caaggcccgg
      421 cccatcccgt tggtttgtcc cggcgtggcg gtggtcgaag aggcgaggga ttgggaagcc
      481 aaattggtca aaaggacgtt ttcctgctgg ctcaggtcgg ccaaggaggc gcccgtcaag
      541 ggcccgccgt ccaggaccga attcgtgaag gacgttcgca ggttctggtc gttgaacccg
      601 gccatggaaa ggcccgcctc gaagaactgg gacccttggg tccccgcgcc ggcggaagtc
      661 accgattggg tcccgaaggc catctggaaa tcgaaggtct cggtggtgga tccgcccaag
      721 gccgccggct gggcgcccgc caccgcttgg cccaaccctt ggaaaagctg gttgaagagg
      781 ttcttgcggg tggattcgtc ggacagcagg gctttttggc tgggcggcag ggattggagg
      841 aagccttgga cggcggccac cagggccttg gccgcgtccg ggctgaccgt gccttgggtg
      901 gcggcgacga tctggccctg cacttggtcg gccgtggcgc ccggattgga caacaggaaa
      961 ccgaccattt gctggaacgc cgaagccagg gcggccatgg actgttggac ctggtccgcg
     1021 ctcgccttgc cccggatcgt ctcggaaaaa cggatgtcga tctgatccag gaaagtggcc
     1081 tggacggcgc tcccccccgg cgtggtttga ccctcggaag actgggcccc gccggccgtg
     1141 gaggccgggg cctgcgacgg cgggttggcc gtggagcc
//
LOCUS       DASTJJ010000401         1491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_667762, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000401 DASTJJ010000000
VERSION     DASTJJ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667762"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1491)
                     /locus_tag="VFR02_04990"
     CDS             complement(<1..>1491)
                     /locus_tag="VFR02_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869834.1"
                     /translation="RAAFLAGLTFALIACAGNFQLMTSALYGGLAFLAYRMFHWSSVE
                     KTLPWRDRWSPRRLLPVLLLGLWGAAPVALQLVPTYQFSQLSTRNQPDYDQLSGTFSM
                     RPSSTYEFLFPDMGLPPGKSAEASIQDIDPPNTDNMSLGDFGYLGVWMPFLAFLAFKR
                     KDRRFLYFLGGMSLLSLLTAWGRYFPLHRILCAVLPGIALSRAPFRFLDVYVLFMVIL
                     SAYGFQALERRFSGGTEEGKPWALGGGAYALLLLAVALFQADRNWREMLGLALGAAGL
                     FGWGFFPGWQRLGRLLFQAALALPLLLSGWGDFGTGPASNYDFDRNAPIFTELQKAPE
                     GSRFFMDMQVPYPVLEDGRDLYFPMPQDEAMNYRVRLVNGYDSINIRRTRDLQNANLG
                     LPVYAALWDVRGLIFGQDRGESDQFNHQVIGTAHYYGLKTPVSHLLAPSRLQVLPDDQ
                     CLAAMRAPGFHPQDQAYLAAPLPADEAAELTGKPAKLSYEWLRDDND"
BASE COUNT          282 a          441 c          571 g          197 t
ORIGIN      
        1 gtcattgtcg tcccggagcc attcatagga aagcttggcg ggcttgcccg tcagctcggc
       61 ggcttcgtcg gccggcaggg gcgcggccag gtaggcttgg tcctgggggt ggaaaccggg
      121 cgcccgcatg gcggccaggc attggtcgtc gggcagcacc tgcagccggg aaggggccag
      181 caggtgggag acgggggtct tgaggccgta atagtgcgcc gtgccgatga cctgatggtt
      241 gaattggtcc gattcccccc ggtcctgccc gaagatgagc ccccgcacgt cccaaagcgc
      301 cgcgtagacc ggcaggccca aattggcgtt ctgcaggtcc cgggtgcggc ggatgttgat
      361 cgagtcgtac ccgttcacca gccgcacccg gtagttcatg gcctcgtcct gcggcatggg
      421 gaaatagagg tcccggccgt cctccaggac gggatagggg acctgcatgt ccatgaagaa
      481 gcgggacccc tcgggggcct tttgcagttc ggtgaagatg ggcgcgttgc ggtcgaagtc
      541 gtagttggag gcgggcccgg tgccgaagtc cccccaaccg ctcagcagca gcggcagggc
      601 gagggcggcc tggaacagca ggcggcccag gcgctgccaa cccgggaaaa agccccagcc
      661 gaagagcccg gcggcgccca gggccaggcc aagcatctcc cgccaattgc ggtcggcctg
      721 gaagagggcg acggccaaga gcagcagggc gtaggccccg ccgcccaagg cccagggttt
      781 gccttcctcc gtgccgccgg aaaagcggcg ctccagggcc tggaagccgt aggccgacag
      841 gatgaccatg aacaggacgt agacatccag gaaacggaag ggggcgcggc tcagggcgat
      901 gcccggcagc accgcgcaga ggatccgatg cagcgggaaa tagcggcccc aagcggtcaa
      961 gagcgacagc agggacatcc ccccgaggaa ataaaggaac cggcggtcct tgcgcttgaa
     1021 ggccaggaag gccaggaagg gcatccagac gcccaggtag ccgaagtcgc ccagggacat
     1081 gttgtccgtg ttgggcgggt cgatgtcctg gatggaggcc tcggcggact tgcccggggg
     1141 caggcccatg tcggggaaca ggaactcgta ggtgctggag ggccgcatgg agaaggtgcc
     1201 ggacagctgg tcgtagtcgg gttggttgcg ggtggagagc tgggagaact gataggtggg
     1261 gaccaactgc agcgccaccg gggccgcgcc ccagagcccc aacagcagca cgggcaggag
     1321 ccgccggggg gaccagcggt cgcgccaggg cagggtcttt tccaccgagg accagtggaa
     1381 catccggtag gccaggaagg ccaggccgcc gtaaagggcg ctggtcatga gctggaaatt
     1441 gcccgcgcag gcgatgaggg cgaaggtgag gccggccagg aaggcggcgc g
//
LOCUS       DASTJJ010000402         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_56446, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000402 DASTJJ010000000
VERSION     DASTJJ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56446"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFR02_04995"
     CDS             <1..766
                     /locus_tag="VFR02_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9869835.1"
                     /translation="QMGRYVHGIHPYSTQHKTEVKEELLQQILYGRTADAVVVVTIRP
                     SEKIVAEYAERKIPMILLENAIPGAHSIRVDNEQGGFLATDYLIRQGRRRIGLINGIT
                     QPPPGVDLVPSAQERLQGYRRALETHGFKFDDGRVVSFGNYLFEEGRQGLQTLLERDP
                     KLDAVFCAGGDIMAMGVMAEAQAQGLKVPQNLALVGYDDMMASSLLKPALTTVRQPIV
                     RMGVEAFETAVDAIDGKLKEDRNTVILPELIVRESA"
     gene            963..>3656
                     /locus_tag="VFR02_05000"
     CDS             963..>3656
                     /locus_tag="VFR02_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9869836.1"
                     /translation="MKTFKSNLLPLFLAALAFLLGVPMAQAQNVNSGGGTYTFPHNAT
                     YPHGFMATGLPSTSASNAVSTTYRDWYGHAVTSGGVTRSGFPTCSSCLRVYRNTNSND
                     TVSEGIGYGMLIAVMMDDKTLFDGLYKYAYQWMPDSSNNMVMDWDLNSGGGIISGNGA
                     TDADEDMAMAFLIASKIWPSAPAVSLTNSQQTSVSKTYQQTYEDMVSAIWTQETKGGA
                     GVYSGDAYQYPYYTSYMTPAWYDCWNSQDTYGRNWAGLSNWVYNTYFKGLSTNSGDNK
                     GFFPDTATQSLTNNTSTMGFDASRYPLRIGIDYLWNGTAGANTYAGTFATNVMNAVNS
                     SGFNTQVGDSWSVPGGTPGGSFCDSLQIGPLAVAAMMTNQANATSAYNQLTARYGSPG
                     CNGGTENFQYFQDTLCMLSELVCTGNFPNIVCGTPPCGSQTCTPTPSPTPLACYMVSD
                     DVDGTEQNHTNGYWFSYWYASVNNTPVVTPVLAGVSNTNYILQANGPSGMPYADAVTG
                     FCAINGGTTLVTYGGSPNQLETVYAGYALGTELKPGYQNLNTMTNISFWVKATTAPAT
                     LQVSFYNPAIDVPAGGNANQYKNVSPIIIAAANTWQYVSIPSGIANIAPSTYAPALGS
                     GGTTYTYSMAMSQVTSLQWQEVAPAAGVTYGFSVGQVCIAGTSWVTPTPLATATPVIT
                     ILPTDTPTLSPTVTRTPTKTVTPTATITATLTYTATPTATTTKTMTPTATATFTPTSA
                     LTPTPTGTPTATASPSPTGTPTASPTATQSPTASNTPTITATRTASSTPTATATATLT
                     ATVTLTPTVSDTPTITSTPTQTTTVFPSSTPTSTPSATATSTFTATPPFSPTPTSTST
                     PAPSATATPTSTSTSTVTLTATRTPTAAFTSTSTSTVTPTQT"
BASE COUNT          740 a         1343 c          955 g          618 t
ORIGIN      
        1 ccagatgggc cgctatgtcc acggcatcca cccctattcc acccagcaca agaccgaggt
       61 gaaggaggag ctgctccagc agatcctcta tgggcgcacg gccgacgcgg tggtggtggt
      121 gaccatccgg cccagcgaaa agatcgtggc cgaatacgcc gagcgcaaga tccccatgat
      181 ccttttggag aacgccatcc cgggcgccca cagcatccgg gtggacaacg agcagggcgg
      241 gttcctggcc accgattacc tcatccgcca ggggcgccgg cgcatcgggc tcatcaacgg
      301 catcacccag ccgccgcccg gagtggacct ggtcccctcg gcgcaggagc ggctgcaggg
      361 ttaccggcgg gccttggaga cccacggatt caagttcgac gacggccggg tggtttcctt
      421 cggcaattac ctctttgagg aaggccggca gggccttcaa accctcctcg agcgggatcc
      481 caagctggac gcggtctttt gcgcaggcgg cgacatcatg gccatgggcg taatggccga
      541 ggcccaagcc cagggcctga aggtgcccca aaacctggcc ctggtgggct atgacgacat
      601 gatggcctcc agcctgctca aacccgccct caccaccgtg cgccagccca ttgtgcgcat
      661 gggcgtggaa gcctttgaaa ccgccgtgga cgccatcgat ggtaagttaa aagaagaccg
      721 caataccgtg atcctgcccg agttgatcgt gcgggaatcg gcttagaaga gctgttttta
      781 acgaaaaaac gtccgctttt tgaggcggcc ggacttttcc ccgcaaaaaa cctttaattt
      841 tgaccctggc cctggcgaaa acaaaaggct gcggtatact tgcaaagttc tcctttacaa
      901 gaaccatcgg gatggatagt ctttgactcc cgttgctaaa aaagaccttt ggataaaatc
      961 gtttgaagac cttcaaatcc aatttattgc ccttgttcct ggccgccctg gctttcctgt
     1021 tgggtgtccc gatggctcag gcccaaaacg tcaactcggg cggcgggacc tacaccttcc
     1081 cgcataacgc cacctatccc catggcttca tggccaccgg gcttcccagc acctccgcca
     1141 gcaacgccgt ttccaccact tatcgggatt ggtatggcca tgccgtcacc agcggcgggg
     1201 tcacccgcag tggcttcccc acctgttcca gctgcttgag ggtctaccgc aacaccaact
     1261 cgaacgatac ggtgtccgag ggtatcggtt acggcatgct catcgcggtc atgatggacg
     1321 acaagacctt gttcgacggc ctctataaat acgcctatca atggatgccg gactccagca
     1381 acaacatggt gatggattgg gacctcaatt ctggcggcgg catcatcagc ggcaatggcg
     1441 ccacggacgc cgatgaggac atggcaatgg cctttttgat cgccagcaag atctggccgt
     1501 ccgcccccgc ggtcagcctg accaacagcc agcagacttc cgtaagcaaa acgtaccaac
     1561 aaacctatga ggacatggtg tccgccatct ggacgcaaga aacgaaaggc ggggcggggg
     1621 tctattcagg ggatgcctac caatatccct actacaccag ctatatgacc ccggcttggt
     1681 atgattgctg gaattcacag gacacttacg gccgcaactg ggcgggcctg tcgaactggg
     1741 tttataacac ctatttcaag gggctctcga ccaattcggg cgacaacaag ggctttttcc
     1801 ctgacacggc cacccagtcc ctgaccaaca atacctccac catgggtttt gacgcttctc
     1861 gctatccgct tcgcattggc atcgactacc tctggaacgg cacggcaggc gccaacacct
     1921 acgccggcac cttcgccacg aacgtcatga acgcggtcaa ctccagcgga ttcaacaccc
     1981 aagtggggga tagctggtcc gtgcccggcg gcacgcccgg cggttccttc tgcgattcgc
     2041 tccagatcgg gcccttggcc gtggccgcca tgatgaccaa ccaagccaac gccacctccg
     2101 cctacaacca gttgaccgcc cgctatggtt cccccggctg caatggcggg acggagaatt
     2161 tccaatattt ccaggacaca ctgtgcatgc tcagcgaact ggtctgcacg ggcaattttc
     2221 ccaacatcgt gtgcggcaca ccgccttgcg gcagccagac ctgtacgccg accccctcgc
     2281 ccacgcccct ggcctgctac atggtctcgg atgatgtgga cggcaccgag cagaaccaca
     2341 ccaacggcta ctggttttcc tattggtacg ccagcgtgaa caatacgccg gtggtcaccc
     2401 ccgtcttggc cggggtctcc aacaccaact acatcctcca ggccaacggc cccagcggca
     2461 tgccctatgc cgacgcggtc accgggttct gcgcgatcaa cggcgggacc accctggtca
     2521 cctacggcgg ttcccccaac caattggaga cggtctacgc gggttacgcc ctgggcaccg
     2581 agctcaagcc cggttaccag aacctgaaca ccatgaccaa catcagtttc tgggtgaagg
     2641 ccaccaccgc cccggccacc ctgcaggtca gtttctataa cccggccatc gacgtgcccg
     2701 cgggcggcaa tgccaaccag tacaagaacg tctcccccat cattatcgcc gcggccaaca
     2761 cctggcagta tgtctccatc ccctcgggca tcgccaacat cgcgcccagc acctacgccc
     2821 ccgcgcttgg gtccggtggg acgacttata cctattccat ggctatgagc caggtgacgt
     2881 cgctgcagtg gcaggaagtg gcgcccgccg ccggggttac ctacggattt tccgtgggcc
     2941 aggtctgcat cgccggcacc agttgggtga cgcccacgcc cctggccacg gccacgcctg
     3001 tgatcaccat cctgcccacc gacacgccca ccctgtcgcc caccgtgacc cggacgccca
     3061 ccaagacggt cacacccacg gccaccatca ccgccacctt gacctacacc gccacgccca
     3121 cggccaccac caccaagacc atgacgccca ccgccacggc caccttcacg ccgacctcgg
     3181 ccctcacgcc cacgccgacg ggcacgccca cggccacggc ttcgcccagc ccgaccggga
     3241 ccccgaccgc cagccccacg gcgacccaga gccccacggc ctccaacacg cccacgatca
     3301 cggccacccg cacggcttcc tcgacgccga cggccaccgc caccgcgacc ctgacggcca
     3361 ccgtgacctt gaccccgacg gtgtcggaca cgcccacgat cacctcgacc cccacgcaga
     3421 ccacgacggt attcccctcg tccacgccca cgtccacccc gagcgccacg gcgacctcca
     3481 cctttaccgc cacgccgccc ttctcgccca cgccgacctc gacctccacc ccggccccga
     3541 gcgccacggc gacgccgacc tcgacctcca cctccaccgt gaccctgacc gcgacccgca
     3601 cgcccacggc cgccttcacc tcgacgtcca cgagcaccgt caccccgacc cagacc
//
LOCUS       DASTJJ010000403         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_56448, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000403 DASTJJ010000000
VERSION     DASTJJ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56448"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..131
                     /locus_tag="VFR02_05005"
     tRNA            57..131
                     /locus_tag="VFR02_05005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89..91,aa:Gly,seq:gcc)
     gene            176..250
                     /locus_tag="VFR02_05010"
     tRNA            176..250
                     /locus_tag="VFR02_05010"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Cys,seq:gca)
     gene            312..1850
                     /locus_tag="VFR02_05015"
     CDS             312..1850
                     /locus_tag="VFR02_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HET9869837.1"
                     /translation="MNPSWTPDDLAQMRDLGISPGEVERQISLFKDPPPFARLARPAA
                     LGDGIRSISGTEKEALLERYGAARDAGRLSKFVPASGAATRMFKTLMGLEAEGATRRA
                     DLEKKAEAGDQEALSALLLMDALPQVAFYADLKKAVAAGGGDLDALLRSGDFGPVWRC
                     LLSEEGLGYGSRSKGLIQFHCHRDGPRTALEEHLWEGLRTVKDARGDCRLHFTVSPEH
                     RAEYEGRVKRLAEAIGAREGARFLVDFSEQGKSYQTLAVDLDDRPFRDADGRLLFRPA
                     GHGALLPNLAGLGADIVFIKNIDNVVVEEMLGPTLEWKKLLAGYLLEVQEGVHGHLAR
                     LEAGVPGAAAEAAEFIRRTLGAVPPASEEGVRKFLDRPLRVCGVVPNTGEPGGGPFWV
                     RDAEGKESLQIVEGAQVDPDSAEQRAIFRRSTHFNPVDLVCALRGPGGRPYALDPFVD
                     PSAVFISRKSHQGRDLKALELPGLWNGSMAGWITVLLEVPASTFNPVKTVFDLLKPAH
                     GGRS"
     gene            1847..>2126
                     /locus_tag="VFR02_05020"
     CDS             1847..>2126
                     /locus_tag="VFR02_05020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9869838.1"
                     /translation="MSAPRGRSILVTGGAGFIGSNFTRYLLGRGGAARVTVLDKFTYA
                     GHPATLRDLARDPRFHLVKGDIADAPLVRRVMRAAKPDWVVNFAAESHV"
BASE COUNT          315 a          718 c          740 g          353 t
ORIGIN      
        1 gcgcgccgtg gttgcccgtg accggtgcgt ttgtgtagag ttaggggtca ggcagggcgg
       61 gaataactca gtggtagagt gcgaccttgc caaggtcgaa gtcgcgggtt cgaatcccgt
      121 ttcccgctcc aatcttcact cgccgccgcg acccctcccg cccggtatcc gattcggcgc
      181 cgtcgccaag tggtaaggca gaggtctgca aaaccgcgat tcggcggttc aaatccgcct
      241 gccgcctcca ttttccgttc accgcttttc catccctttt ttcccacccc caacgccttc
      301 ccggtcgggt catgaacccg tcctggaccc ccgatgacct ggcccaaatg cgcgacttgg
      361 ggatctcccc cggggaagtc gagcgccaga tctccctttt caaggatccg ccccccttcg
      421 cccgtttggc gcggcccgcg gcgttggggg acggcatccg gtccatctcc ggaactgaaa
      481 aggaagcctt gctggaacgc tacggggcgg cccgggacgc cggacggctt tccaagttcg
      541 tgcccgcctc cggcgccgcc acccgcatgt tcaagaccct gatgggcctg gaggccgagg
      601 gggccacccg gcgggcggac ctggaaaaaa aagccgaggc gggggaccag gaagccttga
      661 gcgccttgtt gttgatggac gccctgcccc aggtggcctt ctacgccgac ctcaagaagg
      721 ccgtggccgc cgggggcggg gacctggacg ccctgctgcg ttcgggcgac ttcgggcctg
      781 tctggcgttg cctcctgtcc gaggaagggt tgggctacgg cagccggtcc aaggggctca
      841 tccagttcca ttgccaccgg gacggccccc gcacggccct ggaagaacac ctttgggagg
      901 gcctgcgcac cgtgaaggac gcgaggggcg actgccggct gcatttcacc gtgtcgcccg
      961 aacaccgggc ggagtatgag ggccgggtga agcgcctggc ggaggccatc ggggcgcggg
     1021 aaggcgcgcg gttcctggtg gatttctccg agcagggcaa gtcctaccag accctggcgg
     1081 tggacctgga cgaccggccc ttccgggacg ccgacggccg cctcttgttc cgcccggctg
     1141 ggcatggcgc gctcctgccc aacctggccg ggttgggcgc ggacatcgtc ttcatcaaga
     1201 acatcgacaa cgtggtggtg gaggagatgc tgggccccac gctggaatgg aagaaactgc
     1261 tggccgggta cctgctggag gtccaggagg gcgtccatgg ccacctggcc cggctggaag
     1321 cgggggtccc gggcgccgcc gcggaggccg ccgaattcat ccgccggacc ctgggcgcgg
     1381 tcccgccggc ttccgaggag ggcgtccgga aattcctgga ccgtccctta cgggtctgcg
     1441 gcgtggtgcc caacaccggg gaacccgggg gcggcccctt ttgggtgcgg gacgccgagg
     1501 ggaaggaatc cctccagatc gtggaaggcg cccaggtgga ccccgattcg gccgagcagc
     1561 gcgccatctt ccgccgctcc acccatttca accccgtgga cctggtctgc gccctgcggg
     1621 ggcccggcgg ccggccctac gcgctggatc ccttcgtgga cccctcggcg gtcttcatca
     1681 gccgaaaatc ccaccaggga cgggacctga aggcgctgga actgcccggg ctttggaacg
     1741 ggtccatggc gggttggatc acggtgctcc tggaagtgcc cgcctcgacc ttcaacccgg
     1801 tcaagacggt cttcgacctc ttgaagccgg cccatggggg acggtcttga gcgcgccgcg
     1861 cggcaggtcc atcctggtca cgggcggggc ggggttcatc ggctccaact tcacccggta
     1921 cctattgggc cgtggcgggg cggcgcgcgt gacagtgctg gacaagttca cctacgccgg
     1981 gcacccggcc accctgcggg acttggcgcg ggacccccgg ttccatctgg tgaagggcga
     2041 catcgccgac gccccgctgg tacggcgcgt gatgcgggcg gccaagcccg attgggtggt
     2101 gaacttcgcc gccgaaagcc acgtgg
//
LOCUS       DASTJJ010000404         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1157377, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000404 DASTJJ010000000
VERSION     DASTJJ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157377"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFR02_05025"
     CDS             <1..777
                     /locus_tag="VFR02_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9869839.1"
                     /translation="MVKHAYSLKKPRQLKDAVDFLCEGFHLVEEALASGLNLRYVFAT
                     AEAEKTGAGQELKAECHRQKVRWVPVHPKIIAYVSETAAPQGLLAVGEKPKPRPPQEG
                     SDLVLCLDQVQDAGNVGTLLRSAEAFGAGGVFLTEGCCDLFNPKVVRSAMGALFRLPF
                     QQGMTWEAVLEWSKPNGFSSLALTSHGADPLLEAALPAKTALWVGSEGAGLPEALMAA
                     CDARVCIPMTGRVESLNAAVAGSLALFWVRHGRAGRPPVS"
     gene            849..>1270
                     /gene="pheS"
                     /locus_tag="VFR02_05030"
     CDS             849..>1270
                     /gene="pheS"
                     /locus_tag="VFR02_05030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014909.4"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9869840.1"
                     /translation="MVERIQEILKAAQAAAAQATTLKEVEEARVKFLGKSGELTNLLK
                     ELGKVPAAEKPQMGKALNEAKNAVVALLDARKEAFEEQEFQKADPAWDPTLPGRARPL
                     GRLHPLTKVQKEICSIFKEFGFDQVEGPDVETDHYNFLA"
BASE COUNT          214 a          408 c          438 g          210 t
ORIGIN      
        1 atggtgaagc acgcctattc gctgaaaaag ccccgtcaac tcaaggacgc ggtggatttc
       61 ctctgtgagg gtttccacct ggtggaggag gccctggctt cgggcctgaa cctgcgttat
      121 gtcttcgcca ccgccgaggc cgagaagacc ggggcgggac aggaactcaa ggccgagtgc
      181 caccgtcaaa aggtgcggtg ggtgcccgtc catcccaaga tcatcgccta cgtctccgag
      241 accgccgccc cccaggggct cttggcggtg ggggaaaagc ccaagcccag gcccccccag
      301 gaggggtcgg acctcgtcct ctgcctggac caggtccagg acgcgggcaa cgtgggcacg
      361 ttgttgcggt ccgccgaggc cttcggggcg ggcggggtct ttttgaccga aggctgctgc
      421 gacctgttca accccaaggt ggtgcgttcg gccatggggg cccttttccg cctgcccttc
      481 cagcagggga tgacctggga agcggtcctg gaatggtcga agcccaacgg cttcagttcc
      541 ctggcgctga ccagccacgg cgcggacccg ctcctggagg cggccctgcc ggccaagacc
      601 gccctatggg tggggtccga aggggccggc ctgccggagg ccctgatggc cgcctgcgac
      661 gcccgggtgt gcatccccat gacgggccgg gtggagtcat tgaacgcggc ggtcgcgggc
      721 agtttggcgc ttttttgggt gcggcatggg cgggccgggc gtccgccggt ttcttgacgg
      781 cccacttttt gggatgttaa actcgatttc ttgcgcttgg tttcatcagt cctagtccgg
      841 gagaacccgt ggtcgaacgc atccaagaga tcctcaaggc cgcccaggcc gccgcggccc
      901 aggccacgac cctcaaagag gtcgaggagg cccgggtgaa gttcctcggc aagagcgggg
      961 aactgaccaa cctgttgaag gaactgggca aggtgcccgc cgccgagaag ccccagatgg
     1021 gcaaggcctt gaacgaggcc aagaacgccg tcgtggccct gttggacgcg cgcaaggagg
     1081 ctttcgagga acaggagttc cagaaggccg accccgcctg ggacccgacc ctgccgggcc
     1141 gcgcccggcc cctggggcgc ctgcaccccc tcacgaaggt gcagaaggag atctgctcca
     1201 tcttcaagga gttcggcttc gaccaggtcg agggcccgga cgtggagacc gaccactaca
     1261 acttcctggc
//
LOCUS       DASTJJ010000405         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_56873, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000405 DASTJJ010000000
VERSION     DASTJJ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56873"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFR02_05035"
     CDS             complement(<1..292)
                     /locus_tag="VFR02_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET9869841.1"
                     /translation="MRDRLYLLLASVFGIGYLPWMPGTWTSLAAIPLWWPLLDKPWIL
                     LAALTALFFLGVYLTGEVERIDGKKDPHYASIDELLGMGVALLFLPSPDPGLR"
     gene            complement(301..948)
                     /gene="pgsA"
                     /locus_tag="VFR02_05040"
     CDS             complement(301..948)
                     /gene="pgsA"
                     /locus_tag="VFR02_05040"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9869842.1"
                     /translation="MPTPPRKWNAANLVTVSRVLMVPFFLVLFFRPEAWAKWAAGLVF
                     AWGAYSDYLDGYLARKYKLTTSFGMLLDPLADKVLFLSVMVAFVQQQLAPAWMVIIIA
                     AREFLITGLRQVAQGKNVIIAASRAGKHKTVSQIVAISVILAAVCVRATVEATQGQWD
                     QVLSRWGGWGDALEDFVVYGPYWLMFYATVMSIVSGIDYVVRNRHVLGGDARPPA"
     gene            complement(966..>1328)
                     /locus_tag="VFR02_05045"
     CDS             complement(966..>1328)
                     /locus_tag="VFR02_05045"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9869843.1"
                     /translation="LRQTQMDYVGFFAYSQEENTPAGAMEGQVEEAVKRERLARAYRM
                     QEEIAEKSHRRWVGEKLRAVVESVDGKGLVYGRTKYQAPEVDGVVLVEGLSRPKPGQW
                     LDLEITHTLQLDLVGRAV"
BASE COUNT          246 a          452 c          423 g          207 t
ORIGIN      
        1 gccgcaggcc cgggtccggg ctgggcagga aaagcagcgc cacccccatg cccaacagct
       61 cgtcgatgga ggcgtagtgg gggtccttct tgccgtcgat cctctccacc tcccctgtca
      121 ggtacacgcc caggaaaaag agggccgtca gcgccgccag caggatccag ggcttgtcca
      181 acaggggcca ccaaagcggg atcgccgcca gcgaggtcca ggtgcccggc atccaaggca
      241 gataaccgat gccgaagacc gaggccagca gtaaataaag gcggtcgcgc atgatccctc
      301 ctaggccggg ggccgggcgt cgccgcccag cacgtgccgg ttgcgtacca cgtagtcaat
      361 gcccgagacg atggacatca cggtggcgta gaacatcagc caataaggcc catagaccac
      421 gaaatcctcc agggcgtcgc cccagccccc ccatcgggac aggacctggt cccattggcc
      481 ctgggtggct tccaccgtgg cacgcacgca cacggccgcc aagatgaccg agatggccac
      541 gatctgggac acggtcttgt gtttgcccgc ccggctggcg gcgatgatga cgttcttgcc
      601 ctgggccacc tgccgcaggc cggtgatgag gaattcccgg gccgcgatga tgatgaccat
      661 ccaggccggc gccagttgct gctgcacgaa ggccaccatc accgagagga aaagcacctt
      721 gtccgccagg gggtccagca acatgccgaa gctggtggtg agcttgtatt tgcgggccag
      781 gtagccgtcc aggtagtcgc tgtaggcgcc ccaggcgaag accaatccgg ccgcccactt
      841 ggcccaggcc tccggccgga agaaaagcac caggaagaaa ggcaccatga gcacgcgcga
      901 aacggtcacc aggttggcgg cgttccattt cctcgggggg gtgggcatgg cggcctccgg
      961 acccgtcaaa cggcccggcc gacgaggtcg agttggaggg tgtgggtgat ctcaaggtcg
     1021 agccactgcc ccggcttggg ccgggagagc ccctccacca gcaccacgcc gtccacctcc
     1081 ggggcctggt atttcgtgcg gccatagacc aatcctttgc cgtcgacgga ctcgaccacg
     1141 gcccgcagtt tctcgcccac ccaccgccgg tgtgactttt cggcgatctc ttcctgcatc
     1201 cggtaggccc gggccaagcg ctcccgcttg acggcctcct ccacctggcc ttccatggct
     1261 cccgcggggg tgttctcctc ctgggaatag gcgaagaaac ccacgtagtc catctgggtt
     1321 tggcgcag
//
LOCUS       DASTJJ010000406         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_668389, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000406 DASTJJ010000000
VERSION     DASTJJ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668389"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1745)
                     /locus_tag="VFR02_05050"
     CDS             complement(138..1745)
                     /locus_tag="VFR02_05050"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9869844.1"
                     /translation="MAPILGTPTPRFLSAEPKVTLVNSFTKPFDNAVATARTCYSSKG
                     IVTAEQVAGEGLDEAKQAERRQRRDDLARSIYKAGHHTTLQHAHFQFALENVSRQFIW
                     SFLHSHPFYNSEQVSQRYVTVAPDQVARPPLAGPAAALYDETVAFQTKAYQDFSEKLK
                     GLVGSEYVKRFKGIDLKDKKGATAVQKKSQEVARYILPVATFAYLYHTVSGLTLLRYW
                     RLCQTFDAPLEQRLVVEKMVQAVLEQAPEYQAILEQPMDFESTPEAQIYAGFRATAAT
                     HKAFRDEFDASLGGLTSKLVDHSANGEALLASAVREVFGLPASALSDDEAIALALDPA
                     KNKYLGETLNVSTHSKLSRALFHPHYTFRKKITHAGDSQDQRHRMTPASRPLLHALTL
                     DEPDYLVPVLVRQDPALAREFAGIMDRTWEAIGKLRKLGVDDEYAAYLLPNAVSVRFT
                     ESADLLNLHHKHAMRLCYNAQEEIWAASVDEARQVRQAQPRIGKWLLPPCGLRSRAGT
                     LPKCPEGERFCGVVVWKKDLNDYQRVI"
     gene            1926..>2211
                     /locus_tag="VFR02_05055"
     CDS             1926..>2211
                     /locus_tag="VFR02_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869845.1"
                     /translation="MPTRTRRPKPPRPAGFFSTDTPVLALLFLAVVAVYLSTLCPIFM
                     DDDSAETITAGVTLGLQHPPCYPLDALLNRLAWFLPLGGPGFRAGVGSAFW"
BASE COUNT          319 a          713 c          770 g          409 t
ORIGIN      
        1 ccccctggcg ggggccacga agggcacaaa gttttaagca acccttaaaa aatgatcccc
       61 gtaaatgatc cgttgatgcc gtacttcgtg aactttgtgg cccccgtcag ggggctcgtg
      121 gtggaacagg ttttgcctta gatgacccgc tggtagtcgt tcaggtcctt tttccagacc
      181 accacgccgc aaaagcgctc gccctcgggg catttgggga gcgtcccggc ccggctccga
      241 agcccacagg ggggcaggag ccacttgccg atgcgcggct gcgcttggcg cacctggcgc
      301 gcctcgtcca ccgaggccgc ccagatctct tcctgggcgt tgtagcaaag ccgcatggcg
      361 tgcttgtggt gcaggttgag caggtccgcc gactcggtga agcgcaccga gaccgcgttg
      421 ggcaggagat aggccgcgta ctcgtcgtcc acccccagct tgcgcagctt gccgatggct
      481 tcccaggtgc ggtccatgat ccccgcgaat tccctcgcca aggccgggtc ctgccgcacc
      541 aggaccggca ccaggtagtc gggctcgtcc agcgtgagcg cgtgcaacag gggccgggag
      601 gccggggtca tgcggtggcg ctggtcctgc gagtcgcccg cgtgggtgat cttcttgcgg
      661 aaggtgtagt gggggtggaa gagggcgcgg gagagcttgc tgtgggtgga cacgttcagg
      721 gtctcgccca ggtatttgtt cttggccgga tccagggcca gggcgatggc ctcgtcgtcg
      781 gacaaggccg atgcgggcag gccgaagacc tcccgcaccg ccgaggccaa gagcgcctcg
      841 ccgttggccg aatggtccac cagcttggac gtgagcccgc ccagggaggc gtcgaactcg
      901 tcccggaagg ccttgtgggt ggccgcggtg gcgcggaagc ccgcgtagat ctgggcctcg
      961 ggggtggact cgaagtccat gggttgttcc aggatggcct ggtactcggg cgcctgctcc
     1021 aacacggcct gcaccatctt ctccaccacc aggcgctgtt ccagcggcgc gtcgaaggtc
     1081 tggcagagcc gccagtagcg cagcagcgtc aaccccgata ccgtgtggta gaggtaggcg
     1141 aaggtggcca ccggcaggat gtagcgggcc acttcctggg atttcttctg cacggcggtg
     1201 gcgcccttct tgtccttcag gtcgatgccc ttgaagcgct tgacgtactc gctgcccacc
     1261 aggcccttga gcttctcgga gaaatcctgg taggccttgg tctggaaggc cacggtttca
     1321 tcatagagcg cggcggcggg ccccgcgagc ggcggccggg ccacctggtc gggggccacc
     1381 gtcacgtagc gttgggacac ctgctcggag ttgtagaagg gatggctgtg caggaagctc
     1441 cagatgaact gccgcgacac gttctccagc gcgaactgga aatgggcgtg ctgcagggtg
     1501 gtgtggtggc cggccttgta gatggaccgg gccaggtcgt cgcggcgctg gcggcgctcg
     1561 gcctgcttgg cctcgtccag cccctcgccc gccacctgtt cggcggtcac gatgcccttg
     1621 ctcgaatagc aggtgcgggc cgtggccacc gcgttgtcga agggcttggt gaagctgttg
     1681 accagggtca ccttgggttc ggcggaaagg aaccgggggg tcggggttcc caggatgggg
     1741 gccatgcggg tttccttaac gatgggatgg gcggtggaac cggcgaaggg gtgcgttgcg
     1801 cccccattct atccgagcgc gccgggccgc cggaattatt ttttccgcgc gggtgaattg
     1861 gccctggccc ccgggcccgg ccccggggat aatgggcgca tcccgacgcc caaggggaac
     1921 ccggtttgcc cacccgaacc cgccgcccga aaccgccccg cccggcgggc tttttttcca
     1981 ccgacacccc ggtcctggcc ctcctcttcc tggccgtcgt cgccgtctac ctgtcgaccc
     2041 tctgccccat cttcatggac gacgactcgg ccgagaccat caccgcgggc gtgaccctgg
     2101 ggctgcaaca cccgccctgc tatcccttgg acgccctgtt gaaccgcctg gcctggttcc
     2161 tgcccttggg cggcccgggt ttccgcgccg gcgtggggtc cgccttttgg g
//
LOCUS       DASTJJ010000407         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_789418, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000407 DASTJJ010000000
VERSION     DASTJJ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789418"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..399)
                     /gene="rplV"
                     /locus_tag="VFR02_05060"
     CDS             complement(49..399)
                     /gene="rplV"
                     /locus_tag="VFR02_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET9869846.1"
                     /translation="MQAQAISRFTRLTPRKARQVVELIRRKPVAEALNILAFTKKRAA
                     GEIAKIVKAAVSNAGQNSRVDLDKLRIKTIQVDGGPMLLNAKRFIPRAMGRASAIHRR
                     TSHIKIVVTDETKG"
     gene            complement(413..700)
                     /gene="rpsS"
                     /locus_tag="VFR02_05065"
     CDS             complement(413..700)
                     /gene="rpsS"
                     /locus_tag="VFR02_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9869847.1"
                     /translation="MARSLKKGPYVVEKLLKLVQKMNRANDKKVVKTYARRSTVHPEM
                     VGHTLAIHNGKKFIPVFITENMVGHKLGEFAPTRTFKSHGAVKTEKAMQLK"
     gene            complement(715..1539)
                     /gene="rplB"
                     /locus_tag="VFR02_05070"
     CDS             complement(715..1539)
                     /gene="rplB"
                     /locus_tag="VFR02_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9869848.1"
                     /translation="MPIKNYKPTSPGRRSMTAITGENLTKKAPEKSLMEPLRKTGGRN
                     MYGRITSRHRGAGHKRQYRLIDFTRTKTGMAGKVVSLEYDPNRSSRIALIQYLDGEKK
                     YILAPEGLKVGDQVISGQGADVKPGHAMKLKDIPFGSMIHNIELRPGKGGQMVRSAGS
                     AAQLMAREGEYATVKLPSGEMRKVHIECTASIGQVGNLDHFNVSIGKAGRMRWMGERP
                     QSRGVVTRPGDGHPMGGGEGKSSGGRHPTTPWGKPTKGYKTRQNKMTDKYIVKRRK"
     gene            complement(1542..1838)
                     /gene="rplW"
                     /locus_tag="VFR02_05075"
     CDS             complement(1542..1838)
                     /gene="rplW"
                     /locus_tag="VFR02_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9869849.1"
                     /translation="MKKLQEVIVRPLITERSTALKEQGKYSFEVHPDATKQEIRNAVE
                     SIFKKEKVEVVKVNTINVPGKIRRIGRNVSKPFRWKKAIVTVKPGQKIEFFEGA"
     gene            complement(1835..2470)
                     /gene="rplD"
                     /locus_tag="VFR02_05080"
     CDS             complement(1835..2470)
                     /gene="rplD"
                     /locus_tag="VFR02_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9869850.1"
                     /translation="MPKANLYNSTGKSTGTIDLPENVFGLKPRSHHMWRGVTTYLSNQ
                     RLGTVSTKTKGDVSGGGKKPFKQKGTGNARQGSSRAVQFVGGGIAHGPKPRDYSLELP
                     KKMRRQALAESLSAKALAGQVLVVDAIQFDAPKTKNATGLLKGLGVETKKCLVVLNGA
                     DANVQKSFRNLPKAKVLSRENLNIFEVVNHEVVVMTRQALETIGAAQEKSK"
     gene            complement(2476..>2605)
                     /gene="rplC"
                     /locus_tag="VFR02_05085"
     CDS             complement(2476..>2605)
                     /gene="rplC"
                     /locus_tag="VFR02_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197190.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9869851.1"
                     /translation="LRVVKVDAENNLLFVKGAVPGADGQIVTVRPSVKKQASKSKG"
BASE COUNT          361 a          822 c          811 g          611 t
ORIGIN      
        1 tgtcccaaaa agagcctccg aggcttgcct caaaatttga aagactgctt aacctttggt
       61 ttcgtcggtc accacgatct tgatgtggct ggtccggcgg tggatcgcgc tggcccgtcc
      121 catggcccgc gggatgaaac gtttggcgtt caacagcatg ggaccgccgt ccacctggat
      181 ggtcttgatg cgcaacttgt ccaggtccac ccgggagttc tgcccggcgt tggacacggc
      241 cgccttgacg atcttggcga tctcgccggc ggcgcgcttc ttggtgaagg ccaggatgtt
      301 caaggcctcc gccaccggct tcctgcggat cagctccacc acctggcgcg ccttccgggg
      361 cgtcaagcgg gtgaatcggg atatggcttg tgcctgcatg ggtcgacctt ccttatttga
      421 gctgcatggc tttttcggtc ttcaccgcgc catgcgactt gaacgtccgc gtcggggcga
      481 attccccgag tttgtggccc accatgttct ccgtgatgaa caccgggatg aacttcttcc
      541 cgttgtggat ggccaaggta tggcccacca tctcgggatg caccgtcgac cgccgggcgt
      601 aggtcttcac gaccttcttg tcgttggcgc ggttcatctt ctgcaccagc ttcaacagtt
      661 tctccaccac ataggggcct tttttcaagg agcgtgccat tggggttccc tttcctattt
      721 acggcgtttg acgatgtact tgtcggtcat cttgttctgg cgggtcttgt agcccttggt
      781 gggcttgccc caaggcgtgg tcggatgacg gccgcccgag ctcttgcctt cgccgccgcc
      841 catggggtgg ccgtcgcccg gccgggtcac gacgccgcgg gactggggcc gttcgcccat
      901 ccagcgcatg cgtcccgcct tgccgatgct cacgttgaaa tggtccaggt tgcccacctg
      961 gccgatggag gccgtgcact cgatgtgcac cttgcgcatc tcaccggagg gcagcttgac
     1021 cgtggcgtac tcgccctcgc gggccatcag ctgggccgcc gaaccggccg agcgcaccat
     1081 ctggccgccc ttgccggggc gcagctcgat gttgtggatc atggaaccga aggggatgtc
     1141 cttgagcttc atggcgtgtc cgggcttcac gtcggcgcct tggcccgaaa tgacctggtc
     1201 gcccaccttc aggccctcgg gggccaggat gtactttttc tcgccgtcca agtactggat
     1261 gagcgcgatg cgggagctgc ggttggggtc gtattccaac gacaccacct tgcccgccat
     1321 tccggtcttg gtgcgggtga agtcgatcaa gcggtactgg cgcttgtggc cggcgccgcg
     1381 gtggcggctg gtgatgcggc cgtacatgtt gcgcccgccc gtcttgcgca ggggctccat
     1441 cagcgacttc tcgggggcct tcttcgtcag gttctcgccc gtgatggccg tcatggaacg
     1501 gcggcccggc gaggtgggtt tatagttctt gatcggcatg gtcaggcccc ttcaaagaat
     1561 tcgatttttt ggccgggctt cacggtcaca atggccttct tccagcggaa gggtttggac
     1621 acgttgcgcc cgatgcggcg gatcttcccc ggcacattga tggtgttcac cttcacgact
     1681 tcgacctttt ccttcttgaa gatgctctcc accgcgttgc ggatctcctg cttggtggcg
     1741 tccggatgga cctcgaagga atacttgccc tgctccttga gcgccgtgct gcgttcggtg
     1801 atcagggggc ggacgatgac ttcttgcagt ttcttcattt gctcttctcc tgggccgcgc
     1861 cgatggtctc caaggcttgg cgggtcatga ccaccacctc atggttgacg acctcgaaga
     1921 tgttcaggtt ctcccgggac agcaccttgg ccttgggcag gttgcggaag ctcttctgca
     1981 cgttggcgtc ggccccgttc aggaccacca ggcatttctt ggtctccacg cccaggccct
     2041 tcaggaggcc ggtggcgttc ttggtcttgg gggcgtcgaa ttggatggcg tccaccacca
     2101 gcacctggcc cgccagggcc ttggccgaca gggattccgc cagcgcctgg cggcgcatct
     2161 tcttgggcag ctccagggaa taatcccggg gcttggggcc gtgggcgatg ccgcccccga
     2221 cgaattggac cgcccggctg gagccctggc gggcgttccc cgtgcccttt tgcttgaagg
     2281 gcttcttgcc gccgcccgac acgtcaccct tggtcttggt gctcaccgtg cccaaccgtt
     2341 ggttggacag gtaggtcgtc acgccgcgcc acatgtggtg ggaacggggc ttcaacccga
     2401 agacgttctc cggcaggtcg atggtcccgg tggacttgcc cgtcgaattg tacaagtttg
     2461 ctttcggcat tttggtcacc ctttgctttt cgaggcttgc ttcttcacgc tgggccgcac
     2521 ggtcacgatc tgaccgtcgg cgccgggcac ggcacccttc acgaacaaca ggttgttctc
     2581 cgcgtccacc ttcaccaccc gcagg
//
LOCUS       DASTJJ010000408         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_789431, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000408 DASTJJ010000000
VERSION     DASTJJ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789431"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFR02_05090"
     CDS             complement(<1..372)
                     /locus_tag="VFR02_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET9869852.1"
                     /translation="MARPRLFLLDGAAVAYRSYYAFIKNPRTTTKGLNTSAVFGFLTT
                     LLMILEKQKPEYFAVVFDTSEPTFRHKMYKEYKANRQEMPEDLGQSFPYIHQMVKALG
                     LPLLAKPGYEADDIIGTLVAKA"
     gene            complement(673..960)
                     /locus_tag="VFR02_05095"
     CDS             complement(673..960)
                     /locus_tag="VFR02_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869853.1"
                     /translation="MPQTVPEIIAKKIEAEAKRLAPLAVIRIDFSNYKNDGVDIYSYA
                     PRKVSDMLRGRLKGVLDAELRKATGIRARLLMEDIENMTEEAKKKYGIPVA"
     gene            complement(1011..1883)
                     /locus_tag="VFR02_05100"
     CDS             complement(1011..1883)
                     /locus_tag="VFR02_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9869854.1"
                     /translation="MTKKALGRGLEALIPKKVAASLEEQKKAGATPIPLARIKPNPYQ
                     PRKTFRAEDLQELVASVKEKGVLQPIVVRRRADGWEIIAGERRFRAAQKLGLLTIPAV
                     EVTASDVESLEIALIENLQRADLSPLEEADGYQRLLDEFQMTQEQVALKVGKDRSSVA
                     NALRLLRLAPEVKALVASGRLSAGHARVVAGLNSPQEQKKLAERILGQGMTVRDAERW
                     LQGQKGPVKKSKALAHGKDPNVAKLEEDLRRWLGTKVTIKTQGRHKGTLCLDYYSLDD
                     LDRILSLFRKAGLR"
     gene            complement(1876..>2518)
                     /locus_tag="VFR02_05105"
     CDS             complement(1876..>2518)
                     /locus_tag="VFR02_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9869855.1"
                     /translation="PQGNATSGVGLDKTELSPNIYQVLMGEADIQQAIQPTELPKLFC
                     LPSNSGLIGAEVELVGLENREGRLKAALDTIRSQFDYFLIDCPPSLGFLTLNALMAAQ
                     SVLVPVQSEYYALEGLSSLMETVQMVRENLNPGLQIEGALLTMYDSRTRLASQVSEEV
                     KKFFGEKVYNTVIPRNVQLSESPSFGKPIILYDIRSSGAQAYLNLTKEMLSHD"
BASE COUNT          444 a          751 c          791 g          532 t
ORIGIN      
        1 ggccttggcg accagggtgc cgatgatgtc gtcggcctcg taaccgggtt tggccaaaag
       61 cggcagcccc agggccttga ccatctggtg gatgtagggg aaggactggc ccaggtcctc
      121 gggcatttcc tggcggttgg ccttgtactc cttgtacatc ttgtggcgga aggtgggttc
      181 ggaggtgtcg aacaccacgg cgaaatactc gggtttctgc ttttccagga tcatcagcag
      241 ggtggtcagg aagccgaaga ccgccgaggt gttgaggccc ttcgtggtgg tgcgggggtt
      301 cttgatgaag gcgtaatagg aacggtaggc caccgccgct ccgtccagca ggaacaacct
      361 gggtctggcc atgggatccc ccgtcatgtc tcaaagatgc gaaaatacag ccgaatcgtc
      421 cacttcacaa agtaccgacg accggaacat tgatgacggc cgatagggcc tgatgcttat
      481 tttcaaatat caggcttcat caatccttat cattgttccc aaagcctggt tttgtttgtt
      541 tatttaaaaa aacatagttc cgaaaatgaa aagcccctcc ccgaagatcg ggaagaggcc
      601 ttgagtttaa ccgaaaggcg ggcccttttc ggcaaccaaa aacgttttaa gcccgcggcg
      661 aaagaaaagc ggtcaggcga ccgggatgcc gtacttcttc ttggcttctt ccgtcatgtt
      721 ctcgatgtct tccatcaaca ggcgggcgcg gatcccggtg gccttgcgca gttcggcgtc
      781 cagcacgccc ttcaggcggc cccgcagcat gtcggagacc ttccgggggg cgtagctgta
      841 gatgtccacc ccgtcattct tgtagttgga gaaatcgatc cggatcacgg caaggggggc
      901 caaccgcttg gcttccgctt cgatcttctt ggcgatgatt tcaggaacgg tttgaggcac
      961 aggcgactcc ttacagattg ataccgggct ttttgcccag gtttttcatt ttatcgcaag
     1021 cccgccttgc ggaaaaggct taaaattcgg tccaggtcgt cgagggaata ataatccaag
     1081 caaagggtcc ccttgtggcg gccctgggtc ttgatggtca ccttggtgcc gagccagcgc
     1141 cgcaggtcct cctccagctt ggccacgttg ggatccttgc cgtgggccag ggccttcgac
     1201 ttcttgaccg ggcccttctg cccctgcagc caacgctcgg cgtcgcgcac ggtcatgccc
     1261 tggcccaaga tgcgctcggc cagttttttc tgctcctggg gcgagttcag ccccgccacc
     1321 acgcgggcat ggccggccga aagcctgccc gaggccacga gcgccttcac ctcgggggcc
     1381 agccgcaaca gccgcagggc gttggccacc gacgagcggt ccttgcccac cttcagggcg
     1441 acctgttcct gggtcatctg gaactcgtcc aggagccgct ggtagccgtc ggcctcttcc
     1501 aagggggaca ggtcggcgcg ctgcaggttc tcgatgagcg cgatctccag cgactccacg
     1561 tcggaagccg tcacttccac cgccgggatg gtcaagagcc ccagcttctg ggccgcccgg
     1621 aagcggcgtt cccccgcgat gatctcccag ccatccgccc ggcggcgcac cacgatgggc
     1681 tgcagcacgc ccttttcctt gaccgaggcc accagttcct gcaggtcctc ggcgcggaag
     1741 gtcttgcggg gctggtaggg gttgggcttg atccgggcca gcgggatcgg cgtggccccg
     1801 gcttttttct gttcttccag ggaggcggcg actttcttgg ggatgagggc ttccagcccc
     1861 cggccgagcg ctttcttagt catggctgag catctccttc gtcaggttca aataggcctg
     1921 tgccccgctg gagcggatgt cgtagaggat gatgggcttc ccgaaggacg gggactcgga
     1981 aagctggacg ttgcgcggaa tgaccgtatt gtacactttt tcgccgaaga atttcttcac
     2041 ttcctcggac acctggctgg ccagccgggt ccgggaatcg tacatggtca aaagggcccc
     2101 ctcgatctga aggcccgggt tgaggttttc ccgcaccatc tggacggtct ccatgagcga
     2161 gctcaggccc tccaaggcgt agtactcgct ctgcaccggc accagcacgg attgggccgc
     2221 catgagcgcg ttcagggtca ggaagcccag cgagggcggg cagtcgatga ggaaatagtc
     2281 gaattgggac cggatggtgt ccagggcggc cttgaggcgc ccctcgcggt tctccagccc
     2341 caccagttcc acctcggcgc cgatgagccc gctgttggaa ggaaggcaga agagcttggg
     2401 cagttcggtg ggctggatgg cctgctggat gtcggcctcg cccatcagca cctggtagat
     2461 gttgggggaa agctcggtct tgtccaaccc caccccgctg gtggcgttgc cctggggg
//
LOCUS       DASTJJ010000409         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_914022, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000409 DASTJJ010000000
VERSION     DASTJJ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914022"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFR02_05110"
     CDS             complement(<1..1109)
                     /locus_tag="VFR02_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869856.1"
                     /translation="MNQEPAVTAPRAWPAWLLALPMAWAVLVFLHYFRSLGRALPALP
                     ELFTAAGPGPGIAVRLGVWWGSAWILLCSAVLVGLVLAWGIRLRRWLGLQAGDPWMGL
                     ALAYGLGILGFDLLWLGAGLAHLWWRPLMGALLALGFAVALGDALRYWRAGDFSWRLP
                     REHRWLTATALLYLLLVLGLGLAPETFYDSMVYHLAIPSDWLMRHGILDCPTNFFSNY
                     PYGAELYFLNGLFLQGTEAAKCLHAAAFFFCALAAGGWARETAGSGAGWLAFGMVLTL
                     PLMALNASTTQVEGVLAFFIVLLLYALWKAFGPAPAGGAWALAAGLLAGGALSVKYTA
                     AVALGAGLLSLGRGIFRPGAMRAWAALAGGAVLLLG"
     gene            complement(1106..>1958)
                     /locus_tag="VFR02_05115"
     CDS             complement(1106..>1958)
                     /locus_tag="VFR02_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869857.1"
                     /translation="ALLLLALGLCLSHMLRFVMPAFPLLFMAWAAWAWERGGVWPRFL
                     AGAGLASALLCLPSLLAVSARYFDGAGVWTGAETRERYLQRMVQDPYEDLALWTAAHL
                     PPDARILLVGDARGLYWRRDFLANSVFDEPALARIARQSAGPAQMAERVRRSGITAAA
                     VNLPEGVRTAEDYQSYGEMTGADWKRLDRYLALYWTPLVSDGRSGVWAVRDVPRTGGA
                     TAVDPFSFFTPRAYDLSRALAGGDIPRAIGDARAQAALFPGGTFWDAQLASLEKKTDH
                     PSGAATP"
BASE COUNT          388 a          713 c          665 g          192 t
ORIGIN      
        1 cccagcaaca ggaccgctcc cccggccagc gcggcccagg ccctcatcgc gcccggacgg
       61 aagatccccc ggcccagcga caacaggccc gcccccaggg ccaccgcggc cgtgtatttg
      121 accgagagcg cccccccggc caagagcccg gcggcgaggg cccaggcccc gcccgccggg
      181 gccggaccga aggccttcca aagggcgtag agcagcagca cgatgaaaaa ggccagcacg
      241 ccctccacct gggtggtgga ggcgttcagc gccatgagcg gcagggtcag caccatcccg
      301 aaggccagcc aaccggcgcc ggaccccgcg gtctcccggg cccaaccccc ggccgccagg
      361 gcgcagaaga aaaaggcggc ggcatggagg cacttcgccg cctccgtgcc ctgcaggaag
      421 aggccgttga ggaaataaag ctccgcgcca taggggtaat tcgagaagaa attggtgggg
      481 caatccagga tgccgtggcg catcagccag tccgagggga tggccaagtg gtagaccatg
      541 gaatcgtaga aggtctcggg cgccagcccc aggcccagca ccaagaggag ataaaggagg
      601 gcggtggcgg tcagccagcg gtgttcccgg ggaagacgcc atgaaaagtc cccggccctc
      661 caataccgca gggcgtcgcc cagggccacg gcaaacccca aggccaaaag cgcccccatc
      721 aaggggcgcc accagagatg ggccaatccg gcgcccagcc acagcaggtc gaagcccagg
      781 atgcccagcc cataggccag ggcgagcccc atccagggat cccccgcctg gaggcccaac
      841 cagcggcgca accggatgcc ccaagcgagc accagcccca ccaggacggc ggagcaaagg
      901 aggatccagg ccgatcccca ccaaacaccc agacggaccg cgatcccggg cccgggaccc
      961 gccgcggtga aaagttccgg cagcgccggc agggcccgcc ccaaggaacg gaagtagtgc
     1021 aggaagacca ggaccgccca ggccatgggg agcgccaaaa gccaggcggg ccaggcgcgg
     1081 ggggcggtga cggcgggttc ctggttcatg gggtcgcggc tcccgaaggg tggtcggtct
     1141 ttttttccag cgaggcgagt tgggcgtccc aaaaggttcc cccggggaaa agggcggctt
     1201 gggcccgggc gtccccgatc gcccggggga tgtcgcctcc cgccaaggcc cggctcagat
     1261 cataggcccg gggcgtgaaa aaggaaaagg gatccaccgc cgtcgccccc ccggtccggg
     1321 ggacgtcacg caccgcccag acgccgctgc gcccgtccga gaccaggggc gtccaataga
     1381 gggccaggta gcggtcgagc cttttccaat ccgcccccgt catttcgccg tagctttgat
     1441 agtcctccgc cgtgcgcacg ccctcgggaa ggttgaccgc cgcggcggtg atccccgacc
     1501 gccggacccg ctcggccatc tgggcggggc cggcgctctg ccgggcgatc cgggccagcg
     1561 ccggctcgtc gaacaccgag ttcgccagga aatcgcggcg ccagtagagc ccccgcgcgt
     1621 cccccaccaa caggatcctc gcgtcgggcg gcaggtgggc ggcggtccaa agcgccaggt
     1681 cctcgtaggg gtcctgcacc atgcgctgca ggtaccgctc ccgggtctcg gcgcccgtcc
     1741 aaacccccgc cccgtcgaaa taacgcgcgc tgaccgccag gagggacggc aggcacaaga
     1801 gggccgaggc caggcccgct cccgccaaaa accggggcca taccccgccc cgttcccacg
     1861 cccaagccgc ccaggccatg aagagcaggg ggaaggccgg catcacgaaa cgcagcatgt
     1921 gcgacaggca aagacccaac gccaacagga ggagcgcg
//
LOCUS       DASTJJ010000410         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_302814, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000410 DASTJJ010000000
VERSION     DASTJJ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302814"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..>1035
                     /locus_tag="VFR02_05120"
     CDS             210..>1035
                     /locus_tag="VFR02_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9869858.1"
                     /translation="MTIAWMVGLPVAGALLIFLSPQNKGGWRLALAVSLADLFICLRY
                     FLAFDPFNGGFQFEERLDLGAWLGFHDSLGLDGVSLGFALLTAFLMPLCVAGDLENRR
                     GRRKTYYACLLLLESAALAAFTATDLFLFFLAWEAVLVCLFYLFRYFGSSRRAPAAFQ
                     FVFFQTVGSLLLLGSFLAVVRQAHTFDYGTLLDQPLPEDLQFWCFLALTAAFLIQVPL
                     PPFHSWMSDLYAEAPLGGAFVLGGIFSSLGVYGLVRFCVPLFPTAAKAFGPWLLLLG"
BASE COUNT           97 a          337 c          352 g          249 t
ORIGIN      
        1 ggcatgccgg cgcctcttgg acgagatcct gttggggggg gtggtgcggt tggtggggcg
       61 gggcggggcg ggctggacgc gggccctgaa gcggggccac ctgggctggg cccaatttta
      121 tttcctctat atcctggcgg gcgcggtcct gctggtcttt ttcgcctttc actgagcggg
      181 accatggaac tttcttcggg ggtgcggcct tgacgatcgc ctggatggtg ggcctgccgg
      241 tggcgggggc cctgctcatt ttcctctccc cccagaacaa gggaggctgg cgcctggccc
      301 tggccgtctc gctggccgat cttttcatct gcctgcgcta tttcctggcc ttcgacccct
      361 tcaacggcgg gttccagttc gaggagcggc tggacctggg ggcctggctg gggttccacg
      421 attccctggg attggacggg gtgtcgctgg gttttgccct gttgacggcc ttcctcatgc
      481 cgctgtgcgt ggcgggcgac ctggagaacc ggcgcgggcg ccgcaagacc tactatgcct
      541 gcctgttgct gctggaatcg gcggcgctgg cggccttcac ggccaccgac ctgttcctgt
      601 tcttcctggc ctgggaggcc gtgctggtgt gcctcttcta cctgttccgt tatttcggtt
      661 cgtcccggag ggcccccgcc gccttccagt tcgtgttctt ccagacggtg gggtccctgc
      721 tgttgctcgg ttcgttcctg gccgtggtgc ggcaagccca caccttcgat tacggcacac
      781 tcttggacca gcccctgccg gaggacctgc agttctggtg cttcctggcc ctgacggccg
      841 ccttcctgat ccaggtcccc ctgccgccct tccacagttg gatgtcggac ctctacgccg
      901 aagcgcccct ggggggcgcc ttcgtcctag gggggatctt ctcctccctg ggggtctacg
      961 ggttggtacg cttctgcgtg ccgctctttc ccaccgcggc gaaggccttc ggaccctggc
     1021 tgctgctgtt gggcc
//
LOCUS       DASTJJ010000411         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1402516, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000411 DASTJJ010000000
VERSION     DASTJJ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402516"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VFR02_05125"
     CDS             <1..969
                     /locus_tag="VFR02_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9869859.1"
                     /translation="MGKDVDLEMAGADVELDRTVIEEMGEPLVHLLRNAVDHGIELPA
                     DREKEGKEPYGVIRLEAKRERSFVVISVADDGRGVDPDVIKAKAITKGLITREEAQRL
                     TDEEAVRLIALPGFSTMDAASEISGRGVGVDVAKTKVESMGGSFRIVSRKGQGTSFFL
                     RFPLTLAIIKALLIRCGDERFAVPVVGIVETLDITPADKKTIQQQEALLLRGEVIPLY
                     HLTELLEMPGAGISRPVETVLVAEVGESRVGILVDEVLGQQEVAIKPLDRVLKGIRGF
                     AGATILGTGRIALILDMSSLIEDLKEKRNQAEHLLRLKKGIHAAQS"
     gene            953..>1131
                     /locus_tag="VFR02_05130"
     CDS             953..>1131
                     /locus_tag="VFR02_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HET9869860.1"
                     /translation="MLLNLDELTPHQIDALKEVGNIGAGHGATALSQLLGKRINITVP
                     NASILPLSELPRLVG"
BASE COUNT          215 a          354 c          391 g          171 t
ORIGIN      
        1 atggggaagg acgtggacct ggagatggcg ggcgcggacg tggagctgga ccgcacggtc
       61 atcgaggaga tgggcgaacc cttggtgcac ctgctgcgca acgcggtgga ccacggcatc
      121 gaactgcccg cggaccggga aaaggaaggc aaggaaccct acggcgtcat ccggctggag
      181 gccaagcggg aaaggtcctt cgtggtgatc tcggtggccg acgacgggcg gggcgtggac
      241 ccggacgtca tcaaggccaa ggccatcacc aagggcctga tcacccggga agaggcccag
      301 cgcctcaccg atgaggaggc cgtgcggctc atcgccctgc cgggtttttc caccatggac
      361 gccgccagcg agatctcggg ccggggcgtg ggggtggacg tggccaagac caaggtggaa
      421 tccatgggcg gcagcttccg catcgtcagc cgcaagggcc agggcaccag tttcttcctc
      481 cgcttccccc tgaccctggc catcatcaag gccctgctca tccgttgcgg cgacgaacgc
      541 ttcgccgtgc ccgtggtggg catcgtggag accctggaca tcacgcccgc ggacaagaag
      601 accatccaac aacaggaagc attgttgttg aggggtgagg tcatcccgct ctatcatctg
      661 accgagctgc tggagatgcc gggggccggc atttcccgcc cggtggagac cgtgctggtg
      721 gccgaggtgg gggagtcccg ggtgggcatc ctggtggacg aggtgttggg ccagcaggaa
      781 gtggccatca agcccctgga ccgggtgctg aagggcatcc gggggttcgc cggcgccacc
      841 atcctgggaa cgggcaggat cgccctcatc ctggacatgt cgagcctgat cgaggacctg
      901 aaggaaaaac gcaaccaagc cgaacacctg ctgcgcctga agaaagggat ccatgctgct
      961 caatcttgac gagctgacgc cgcaccagat cgacgccctc aaggaggtcg gcaacatcgg
     1021 ggccggccac ggggccaccg ccttgtccca gttgctgggc aagcgcatca acatcaccgt
     1081 gcccaacgcc tccatcctgc cgctctccga actgccccgg ttggtgggcg a
//
LOCUS       DASTJJ010000412         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1402555, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000412 DASTJJ010000000
VERSION     DASTJJ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402555"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..>1073
                     /locus_tag="VFR02_05135"
     CDS             81..>1073
                     /locus_tag="VFR02_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HET9869861.1"
                     /translation="MRNYLKWGSRMLMAAVMLLSGMTASRAADDTVKIGILHSLSGTM
                     AISETSLRDAVLMAVGEINAAGGVLGKKIVPVVVDPASNWDLFAEKAKELITQDKVKV
                     TFGCWTSVSRKSVLPVFEANDALLFYPVQYEGEEQSLNVFYTGAVPNQQLIPAAEYLM
                     SPEGGSKKKFFLLGTDYVFPRTANKVLKAFLLAKGVPETNIMEVYTPFHYQDYQTIVS
                     QIKDFAKGGGACVLSTVNGDSNVPFYKEFANQGLTAADCPIMAFSVAEDELRAMDVPP
                     LVGHYACWNYFQSLKTPENAKFVKDFKKYCADNNLPGGSSRVTDDPIEAAYFGVY"
BASE COUNT          220 a          332 c          341 g          180 t
ORIGIN      
        1 gtcgtcgggg accagcacca gcatgatcca gctggggacc caactgatct attaacccaa
       61 acgagcaaag gagaagaacg atgcgcaatt acctgaagtg gggaagtcgg atgttgatgg
      121 cggcggtgat gctgctgtcc gggatgacgg cgtcccgggc ggccgacgat acggtcaaga
      181 tcgggatcct gcattccttg agcggcacca tggccatctc ggaaacgtcc ctccgggacg
      241 cggtcctgat ggcggtgggc gagatcaacg ccgccggcgg ggtgctgggc aagaagatcg
      301 tgcccgtggt ggtggacccg gccagcaact gggacctgtt cgccgaaaag gccaaggagc
      361 tcatcaccca ggacaaggtc aaggtgacct tcgggtgctg gacctcggtc agccgcaagt
      421 cggtgctgcc ggtgttcgag gccaacgacg ccctgctctt ctaccccgtg cagtacgagg
      481 gcgaggaaca gtcgttgaac gtcttctaca cgggcgcggt gcccaaccaa cagctcatcc
      541 cggccgccga atacctgatg agcccggagg gcgggtcgaa gaagaagttc ttcctgctgg
      601 gcacggacta cgtcttcccg cgcacggcca acaaggtgct gaaggccttc ctgttggcca
      661 agggcgtccc cgagaccaac atcatggagg tctacacgcc cttccattac caggactacc
      721 agaccatcgt ctcgcagatc aaggacttcg ccaagggcgg cggggcctgc gtgctttcca
      781 ccgtcaacgg ggacagcaac gtgcccttct acaaggagtt cgccaaccag ggcctgacgg
      841 cggccgattg ccccatcatg gccttctcgg tggccgagga tgagctgcgg gccatggacg
      901 tgccccccct ggtgggccac tacgcctgct ggaactactt ccagagcctg aagacccccg
      961 agaacgccaa gttcgtgaag gacttcaaga aatactgcgc ggacaacaac ctgccgggcg
     1021 gctccagccg cgtgacggac gaccccatcg aggcggctta tttcggggtc tat
//
LOCUS       DASTJJ010000413         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1647439, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000413 DASTJJ010000000
VERSION     DASTJJ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647439"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFR02_05140"
     CDS             complement(<1..436)
                     /locus_tag="VFR02_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869862.1"
                     /translation="MTTRGKKNNNIRALKFQSKPRAASALNLHKGLWSLYHASDDLHL
                     SKPLPALLKTLLKGLKAGTGLGKAAIFLYEESADLLAGTAAVGLGEAKVHALRVTVQR
                     EEVADLMKLHQKGPDAKGSSPIQNYWAQTFQAELGLKSARLLP"
     gene            669..>1093
                     /locus_tag="VFR02_05145"
     CDS             669..>1093
                     /locus_tag="VFR02_05145"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9869863.1"
                     /translation="MTKTLLITGGAGYIGSHAVREAPRFGFKPVVFDNLSAGHAWAVK
                     GAVLVKGDLADRPGILAAFRKFRPAAVMHFASHISVGESVADPQKYYRDNLTNAMNLF
                     SVMLERKVSYFILSSTAATYGDPVRVPMPETHPQVPVNP"
BASE COUNT          200 a          318 c          355 g          220 t
ORIGIN      
        1 gggggagcag ccgggccgac ttgaggccca gttcggcctg gaaggtctgg gcccagtagt
       61 tttggatggg gctggaaccc ttggcatcgg gccccttttg gtgcagcttc atcaggtcgg
      121 ccacttcctc gcgctgcacg gtgacccgca aggcatggac cttggcttcg cccaacccca
      181 cggcggcggt gccggccaac aggtcggcgg attcctcata aaggaagatg gcggccttgc
      241 ccaggccggt cccggccttc aggcccttca aaagggtctt gaggagggcg ggaagcggct
      301 tggacaggtg gaggtcgtcg ctggcgtggt agagggacca gagtccctta tggaggttca
      361 aggccgaggc ggcccggggt ttggattgga atttcaaagc ccggatgtta ttgttctttt
      421 tgccgcgcgt ggtcatggag aaggaccctg ttcttcaaaa caggatttag cgtggagggg
      481 tgtggacggt caattatggg ccagcccccg aggcgcgggt tagccaaaaa ttgagcgtta
      541 tcggaaaatg ttttcccgcc ggaagaagag cgctcaaaat gattttagga agttgttgtg
      601 gaaacacggc atagttggtc ttggcggatc gtgaaaccaa acggcttttg gccggacccg
      661 aggacaccat gacgaagacc ctgctcatca ccggcggcgc cggctacatc ggaagccacg
      721 ccgtgcgcga agcgccccgt ttcggcttca agcccgtggt gttcgacaat ttgtccgccg
      781 gccacgcttg ggccgtgaag ggcgcggtgt tggtgaaggg cgacctggcg gaccggccgg
      841 ggatcctggc ggccttccgc aaattccggc ccgcggccgt catgcatttc gcttcccaca
      901 tctcggtggg tgaatcggtg gccgaccccc aaaagtacta ccgcgacaac ctcaccaacg
      961 ccatgaacct tttctcggtc atgttggaac gcaaggtctc ttacttcatc ctgtcctcca
     1021 cggccgccac ttacggcgac ccggtgcggg tgcccatgcc cgagacccat ccccaggtcc
     1081 cggtgaaccc tta
//
LOCUS       DASTJJ010000414         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1647559, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000414 DASTJJ010000000
VERSION     DASTJJ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647559"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..922)
                     /locus_tag="VFR02_05150"
     CDS             complement(5..922)
                     /locus_tag="VFR02_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869864.1"
                     /translation="MPAPAAPATWSDLYGRAWKVCCDLWPLWLARFFYLILNYGAFLL
                     CLVFTFWPLLSKVLGMVRGGESLGQGDYQALVADFFSNFRDAGFLLPVLAIFFFYLAW
                     WTVISAWFAGGLYARLNAWVKRGEAFSWTDFVKEGFYHLVPMILLQGLLGTVALLGVG
                     AYLIAMVLGGFVLGLAHVPGWAALVALLPAGLVFFAILIFWDCFAMVSQSCLVETDRV
                     FEAFEKGCRACLADGFDLARMVGVLITVFTIVFVAALLFFKLLELIPLVGLFFFLSDV
                     VLRLFVALVSDVYFPALAVVGLHEKEADA"
     gene            complement(932..1432)
                     /locus_tag="VFR02_05155"
     CDS             complement(932..1432)
                     /locus_tag="VFR02_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET9869865.1"
                     /translation="MADTYSFDVVSEVDLQEVDNAVNQAMKEISQRYDFKGSISEVKL
                     NKTAKELEIVADNESRLNAVTDILKGKFIKRNLSPKALDFQKAEAAFSGTLNQKVKVI
                     SGLPMDAAKDIVKRVKDSKLKVQASIVDEKVRISGKSKDDLQAAMQLLRGASDLKVAL
                     QFNNFR"
     gene            complement(1613..>2040)
                     /locus_tag="VFR02_05160"
     CDS             complement(1613..>2040)
                     /locus_tag="VFR02_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HET9869866.1"
                     /translation="IQPRLGPMMRAMHAPPAATAPAATAVPAAPVTAAERPATAAAPP
                     LVAPLPGVKDKYQHLILKGLQQSWVLVTMDDGQSSSEIDLAPGETKSYKALRNFKLRI
                     GNAGGVDASYNGKPLGILGVSGQVVELSLPGGSPVTPKS"
BASE COUNT          426 a          667 c          623 g          324 t
ORIGIN      
        1 agggtcaagc gtccgcttcc ttttcatgca ggcccacgac cgccaaggct ggaaaataca
       61 cgtcgctgac cagtgcgacg aaaagccgca ggacgacgtc cgacaggaag aagaacaacc
      121 ccaccagggg gatcaattcc aacagtttga aaaaaagaag ggcggccacg aagacgatgg
      181 tgaagaccgt gatcagcacc cccaccatgc gcgccaggtc gaatccatcc gccaagcagg
      241 cccggcagcc cttttcgaag gcttcgaaca cccggtcggt ctccaccagg caggactggc
      301 tgaccatggc gaaacaatcc caaaagatca ggatcgcgaa aaagaccagg ccggctggca
      361 ggagcgccac cagggccgcc caacccggaa cgtgggccaa ccccagtaca aaaccgccca
      421 agaccatggc gatcaggtag gccccgaccc ccaagagcgc caccgtgccc aacaggccct
      481 gcaacaggat catgggcacc aggtggtaaa aaccttcctt tacgaagtcc gtccagctga
      541 aggcttcccc ccgcttgacc caagcgttca accgcgcgta gaggcccccg gcgaaccagg
      601 cggagatcac cgtccaccaa gccagataga aaaagaagat ggccagcaca ggcaggagga
      661 atcccgcgtc ccggaagttg gagaagaaat ccgcgaccag cgcctgatag tccccttggc
      721 ccaggctttc cccgccccgg accatcccca gcaccttgga caacaacggc cagaaggtga
      781 acaccaaaca caagaggaag gcgccgtaat taaggatcag gtaaaagaac cgggccaacc
      841 agaggggcca aaggtcgcaa caaaccttcc aggcgcggcc atagaggtcg gaccaggtgg
      901 cgggggcggc gggggcgggc atgggggcct ttcagcggaa attattgaac tgcagggcca
      961 ccttcaggtc ggaggcgccc cgcaacagct gcatcgcggc ctgcaggtca tccttggact
     1021 tgcccgagat ccggaccttc tcgtccacga tggaggcctg caccttcaac ttggaatcct
     1081 tgacgcgctt gacgatgtcc ttggcggcgt ccatgggcag gccgctgatg accttgacct
     1141 tctggttcag ggtcccgctg aaggcggcct cggccttctg gaaatccagg gccttgggcg
     1201 agaggttgcg cttgatgaac ttgcccttca ggatgtcggt cacggcgttg aggcgcgact
     1261 cgttgtcggc cacgatctcc agttccttgg cggtcttgtt cagcttgacc tcgctgatgc
     1321 tgcccttgaa gtcgtagcgc tgggagatct ccttcatggc ctggttgacc gcgttgtcga
     1381 cctcctgcag gtcgacttcc gagaccacgt cgaatgagta ggtgtccgcc atgccgccgc
     1441 cctttcgaag aatttatgtc gggacctttt caccgcaaag acgcagaggc gcaaaatcgg
     1501 aacaacgctc ccggcagggc aaagatccct ccttcccggc cgggacacca agtcccaagg
     1561 gcggcatctt ttgccaaaac cgcggccgcc ccccgtccaa gagggcttcc cgtcaggact
     1621 tgggggtcac gggggacccg cccggaaggg acagctccac cacctggccg ctcaccccca
     1681 ggatgcccag cggcttcccg ttgtaggagg cgtccacgcc cccggcgttc ccgatgcgca
     1741 acttgaagtt cctcagcgcc ttgtagctct tggtctcccc gggcgccagg tcgatctccg
     1801 agctgctctg gccgtcatcc atggtcacca ggacccacga ttgttgcagg cccttcaaga
     1861 tgaggtgttg gtacttgtcc ttcacgccgg gcaagggcgc caccaggggg ggcgcggcgg
     1921 cggtcgcggg gcgttcggcg gccgtcacgg gcgcggcagg gacggccgtg gccgcggggg
     1981 cggtcgccgc cgggggcgcg tgcatggccc tcatcatcgg gcccaatcgt ggctggatcc
//
LOCUS       DASTJJ010000415         1861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1769664, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000415 DASTJJ010000000
VERSION     DASTJJ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769664"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VFR02_05165"
     CDS             complement(<1..85)
                     /locus_tag="VFR02_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9869867.1"
                     /translation="MRIAVIGAGVAGLTAAYRLAQKDHEVDV"
     gene            complement(82..1095)
                     /locus_tag="VFR02_05170"
     CDS             complement(82..1095)
                     /locus_tag="VFR02_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9869868.1"
                     /translation="MLKQKRTWLGIFFIVFTFYFLFRNLNFARLLDNLGHFHIFWILP
                     ALAVYLLGYVLRGYRWVILMSPIKRCSFSSLFPTLIIGFMMNNLLPARLGEIYRAYHN
                     GTKEGVSRSASLATILLERLFDGLTMIVALWAALSFARLPIKVQSMPAGIQHAIQWSP
                     WVFGAAFVFCFALLIFKKTAVATMNYFISHAPAQYHEVLTKIGHTFIDGLQVLQNAKE
                     SIGVMVLSLLAWGCEFTSYYFIAIGFSLAPSPLNLWSAALLMAVANLGILIPNAPGGI
                     GLFEFIGVALLLPYGIPKETAVGYMFLLHFLLLIPITLMGLYFFSREGLRFKDIGKKE
                     MAP"
     gene            1251..>1861
                     /locus_tag="VFR02_05175"
     CDS             1251..>1861
                     /locus_tag="VFR02_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869869.1"
                     /translation="MKRRLAVLLGMLAAGWGGAAVAQTPAPVAPPAFNTFKDAYAAGN
                     EDLRARKFTQAAADYGAAENLAFSDKGKSEAANAQGWAYLQAKDWDNAKGSLSRAVTE
                     DGNNKTALKNLGAACYTRYEYGMAGLDELKTAIDNLKASGDSPELLDRAQADLAREQS
                     YAQVTPGPEPVLKGQSFKALLDLGDQAQAQGRFDLALKAFKQA"
BASE COUNT          407 a          551 c          622 g          281 t
ORIGIN      
        1 aaacgtcgac ctcatggtcc ttctgggcca ggcggtaggc cgccgtcagc cccgccaccc
       61 ccgcgccgat gaccgcgatc ctcatggtgc catttccttt ttaccgatgt ccttgaagcg
      121 caacccttcc cgggagaaga aataaagccc catcagggtg atggggatga gcagcaggaa
      181 atgcagcagg aacatgtagc ccaccgccgt ctccttgggg atgccgtagg gcaggagcaa
      241 cgccacgccg atgaactcga agagcccgat gccccccggc gcgttgggga tgaggatgcc
      301 caggttggcc acggccatca ggagcgccgc cgaccagagg ttgagggggg agggcgccag
      361 ggaaaaaccg atggcgatga agtaatagga ggtgaactcg cagccccagg ccagcagcga
      421 aaggaccatc accccgatgg attccttggc gttctggagg acctgcaacc cgtcgatgaa
      481 ggtgtggccg atcttggtca ggacctcgtg gtattgggcg ggcgcgtgcg agatgaaata
      541 gttcatggtg gccacggcgg tctttttgaa gatcaagagg gcgaagcaaa agacgaaggc
      601 ggcgccgaag acccagggcg accattggat ggcgtgctgg atgcccgccg gcatggactg
      661 caccttgatc ggcaggcggg cgaaggaaag ggcggcccag agggccacga tcatggtgag
      721 cccgtcgaag aggcgctcca acaggatggt ggccagggag gcgctgcggc tgaccccttc
      781 cttggtgccg ttgtggtagg cccggtagat ctcgcccagc cgggcgggga ggaggttgtt
      841 catcatgaac ccgatgatga gggtggggaa aaggctcgaa aaactgcacc ttttgatggg
      901 ggacatcagg atgacccagc ggtagccccg cagcacatag cccaagaggt acacggccaa
      961 ggcggggagg atccagaaga tgtggaaatg ccccagattg tccagcaggc gggcgaagtt
     1021 gaggttccgg aaaaggaaat aaaaggtgaa gacgatgaag aaaatcccga gccaagttct
     1081 cttttgcttg agcatttcgg tcctttgcct atggagggct tattttgcat gaaaagcgcg
     1141 ttttttgtta atcaaaaaag ccccggcccg ttaatttcct gggcaggccc ggcccggccc
     1201 cgtaaaatga aacgcaatgg aagcacatta gaagccgcat ggaggtcgcc atgaagcgcc
     1261 gattggccgt cctgttggga atgctggccg ccggatgggg cggggccgcg gtggcccaaa
     1321 cccccgcgcc ggtcgcgccc cccgccttca acacgttcaa ggatgcttac gccgccggca
     1381 atgaggacct gcgcgcccgc aagttcaccc aggccgcggc cgattacggc gcggccgaga
     1441 acctggcctt cagcgacaag ggcaagagcg aggccgccaa cgcccagggt tgggcctacc
     1501 tgcaggccaa ggattgggac aacgccaagg ggtccctgtc ccgcgcggtg accgaggacg
     1561 gcaacaacaa gactgccctc aagaacctgg gcgccgcctg ctacacgcgc tacgagtacg
     1621 gcatggcggg cctggacgag ctgaagaccg ccatcgacaa cctgaaggcc agcggcgaca
     1681 gccccgagct gctggaccgc gcccaggccg acctggcccg ggaacagtcc tacgcccaag
     1741 tcacgccggg cccggagccc gtcctgaagg gccaaagctt caaggcgctc ctggacctgg
     1801 gcgaccaggc ccaggcccaa ggccggttcg acctggcgct caaggccttc aagcaggccg
     1861 a
//
LOCUS       DASTJJ010000416         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1526669, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000416 DASTJJ010000000
VERSION     DASTJJ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526669"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..>2038
                     /gene="dxs"
                     /locus_tag="VFR02_05180"
     CDS             244..>2038
                     /gene="dxs"
                     /locus_tag="VFR02_05180"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET9869870.1"
                     /translation="MYEHLDKIQSPADLRKLPVEALPKVAEELRDFLVTSVSETGGHL
                     GASLGAVEMTLAMHYVYDTPNDRIVWDVGHQAYGHKALTGRRHLFPTLRQYKGLCGFP
                     KRNESEYDTFAVGHAGTALSAACGMALARDLQKKDFHVLAVVGDAAISNGMAMEAMNN
                     IGDHPNMNLTVILNDNEMSISPSVGAMTSYLNKILTGKTFNQTKRNIEGLIEKVPNLG
                     PQMLKMAHHAEEALKGFVTPGTWFEELGFRYYGPLDGHDLPNLIEILKRVKQLKGPVM
                     LHVVTKKGKGYAPAEENPEKFHGVSVFDKQTGKSVAAAGPSAPTYTKVFGDAMIELAA
                     KDPKLVAITAAMPEGTGLKAFGQRFPAQFIDVGIAEEHAVCMAAGLACEGVKPVVAIY
                     STFLQRAFDQIIHDVCLQKLPVVFALDRGGLVGDDGETHQGVYDLSYLRCVPNMVVMA
                     PSDEAELRDMLHTAVQHDGPIALRYPRGNGEGVPMEAGFKTLAIGKGRLLESGNDYAL
                     LAVGRMVGVAKSARAQLKKEGLEGAVADMRFVKPLDEDLLLSLASRVKVLLTLEENVA
                     AGGFGAAVLECLQRNGVTDCAVKLIGIPDQFI"
BASE COUNT          373 a          672 c          667 g          326 t
ORIGIN      
        1 ccaggtggtg gtgggggcct tcttggggat cgccgtggcc gttttgtttt atacgcttta
       61 ccataagccg ctttcaccac gagcccctgc ggggccacga agggcacgaa gttcaggaaa
      121 agccttttga tgggcgggaa cggcgcagtg aggtcggtgt ttgataaaac agagtccttg
      181 gcttggtgga cttcgtgatc cccgcagggg atttgtggtg aaataggttt ttaaggggcc
      241 tgaatgtacg agcatcttga taagatccaa agcccggcgg acctgcgcaa gctgcccgtg
      301 gaggccctgc ccaaggtggc cgaggaactg cgggacttcc tggtgacctc cgtctccgag
      361 accggcgggc acttgggcgc cagcctgggc gccgtcgaga tgaccctggc catgcattac
      421 gtctacgaca cccccaacga ccgcatcgtc tgggacgtgg ggcaccaggc ctacggccac
      481 aaggccctca ccggccgccg gcaccttttc ccgaccctgc gccagtacaa gggcctctgc
      541 ggttttccca agcgcaacga gagcgaatac gacaccttcg cggtgggcca cgcgggcacg
      601 gcgctttcgg cggcctgcgg catggccctg gcccgcgacc tgcagaagaa ggacttccac
      661 gtgctggccg tggtgggcga cgccgccatc tccaacggca tggccatgga ggccatgaac
      721 aacatcggcg accaccccaa catgaacctg acggtcatcc tgaacgacaa cgagatgtcc
      781 atctcgccct ccgtgggcgc catgaccagc tacctgaaca agatcctcac gggcaagacc
      841 ttcaaccaga ccaagcgcaa catcgaaggg ctcatcgaga aggtccccaa cctggggccc
      901 cagatgctca agatggccca ccacgccgag gaggccctga agggcttcgt cacgcccggc
      961 acctggttcg aggaactggg tttccgctac tacggcccgt tggacggcca cgacctgccc
     1021 aacctcatcg agatcctcaa gcgggtcaag cagctcaagg gcccggtcat gctgcacgtg
     1081 gtcaccaaga aggggaaggg ctacgccccc gccgaggaga accccgagaa gttccacggc
     1141 gtgagcgtgt tcgacaagca gacgggcaag agcgtggccg ccgccggccc ctcggccccc
     1201 acctacacca aggtcttcgg cgacgccatg atcgaattgg cggccaagga ccccaagctg
     1261 gtggccatca cggccgccat gcccgagggc acgggcctga aggccttcgg ccagagattc
     1321 cccgcgcagt tcatcgacgt gggcatcgcc gaggagcacg cggtctgcat ggccgccggg
     1381 ctggcctgcg agggcgtgaa gcccgtggtg gccatctatt ccaccttcct gcagcgggcc
     1441 ttcgaccaga tcatccacga cgtctgcctg cagaaactgc ccgtggtctt cgccttggac
     1501 cggggcggcc tggtgggcga cgacggcgag acccaccagg gcgtctatga cctgtcctac
     1561 ctgcgctgcg tgcccaacat ggtggtgatg gccccctccg acgaggccga actgcgcgac
     1621 atgctccaca ccgccgtcca gcacgacggg cccatcgccc tgcgctaccc ccgcggcaac
     1681 ggggaaggcg tgccgatgga ggccggcttc aagacgctgg cgatcggcaa gggcaggctc
     1741 ttggagtccg ggaacgacta cgccctgctg gccgtgggcc gcatggtggg cgtggccaag
     1801 tccgcgcggg cccagttgaa gaaggagggc ctggagggcg cggtggccga catgcgcttc
     1861 gtgaagcccc tggacgagga cctgctgctt tccctcgcct cgcgggtgaa ggtgctgctg
     1921 accctggagg agaacgtggc ggcgggcggt ttcggcgcgg ccgtgctgga atgcctgcag
     1981 cgcaacggcg tcaccgactg cgcggtcaag ctgatcggca tcccggacca gttcatcg
//
LOCUS       DASTJJ010000417         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_425655, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000417 DASTJJ010000000
VERSION     DASTJJ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425655"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFR02_05185"
     CDS             complement(<1..285)
                     /locus_tag="VFR02_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869871.1"
                     /translation="MRILFFSCFCLLSCGSLWAQTAVLTLDRNVFHPTAGSVVTMTFR
                     ADYSGRGRLVVYNSAGESIRTLLAAGGQVDADHTYRLTWDGKNQAGDDVAP"
     gene            complement(290..>2672)
                     /locus_tag="VFR02_05190"
     CDS             complement(290..>2672)
                     /locus_tag="VFR02_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869872.1"
                     /translation="LSTKPPRPGGRERLIVVPFLASLAFLAACLWGMGAPSFLPVWSD
                     PSAASWSMDWMWKAWASKAAACLEAGGAFLGLAGWAQSFRSFLFGRTLWEEQGALAWG
                     MSLACFSLAVFGLGVNHLLEGPLVALLLAAGIPAGLRWTSGTGWSDWRRWQKALLAAA
                     LLPWLFEFLSPPIIWDAVLDHFRFAREVARLHQVPLHWVNHTGDIPKGAELIWAGFWS
                     MGGEPLARASGFLVPVGVLLLGRSLARSLKLPEWPLFLLVAACPYWLALMGWGYDEGL
                     LAFYELSSCLALVAAFRGPGIRPAAWAAAFFFLGAALGVKYTALFAWVACGSLVLAYG
                     WGQRKIAWDWKWTLFLLAPCLPWLLRNALANGNPFYPMAAAWWGGPSGYDPGLESALW
                     ADTGSPAGLSFLGAARTWLLDFWTAGNQVGAPLAPLILMGVPFWWQLRRDREARALMI
                     FSLVFLVAWTFFCTSLRHAAGAVLAMTFLSGLAWARVLTPSSRKVRWLFAAGLLVSLA
                     SVWAAQSRVTRPYGYALGFQDPMDRLKKNYSMDFDTFSAYEAVEKNSGPRDKTMAFGV
                     FQTYPLRRGAFVDFFWKKPIFLQWASGCRTAGQLAEKLRREGVTTFLYQRAEAAYMSR
                     KEKNFGLSGMPVQAYERFWARYTEPLAVFENSSLYGIRASPLPQDRPLIDLPGVEEPW
                     MADILRADQKGDRSGAYLAAGSLVRAHPEVGEGWERLACAAGALGKWEETVRAGNRAE
                     DLGAESLDLCRTMAEAYDRLGRPKARDRWAQKAQARARWLEGTRRGSTAFERE"
BASE COUNT          608 a          944 c          764 g          356 t
ORIGIN      
        1 gggcgccaca tcatcccccg cctggttttt gccgtcccag gtcaagcggt aggtatgatc
       61 ggcgtcgacc tggcctcccg cggccaggag ggtgcggatc gattctcccg ctgaattgta
      121 gaccaccagc cgtcccctgc ccgaatagtc ggcccggaag gtcatggtga ccacgctccc
      181 ggcggtcgga tggaaaacgt tgcggtccaa ggtcaagacc gcggtctggg cccaaaggga
      241 cccgcaggac agcaggcaaa aacaagaaaa gaacaagatc ctcattcggt cactcccttt
      301 caaaagccgt ggatccccgc cgcgttccct ccaaccaacg ggcccgggcc tgcgcctttt
      361 gggcccaccg gtccctggct ttcggccgac ccaaccgatc ataagcctcg gccattgtcc
      421 ggcataggtc cagggactcc gcccccaggt cctccgcgcg gttccccgcc cggaccgtct
      481 cttcccattt ccccaaggcc ccggcggcgc aggccaggcg ttcccaacct tcccccactt
      541 ccggatgggc ccggaccagg gatcccgccg cgagataggc tcctgaccga tcgccttttt
      601 gatcggcccg gaggatgtcc gccatccagg gctcctccac gccgggcagg tcgatcaagg
      661 ggcggtcctg cggcaagggc gaggccctga tgccgtacag gctggaattc tcgaagaccg
      721 ccaggggttc ggtgtaccgg gcccaaaaac gctcatacgc ctggaccggc atcccgctca
      781 agccgaagtt tttttccttc cgcgacatat aggccgcttc cgcccgctgg taaaggaagg
      841 tggtcacccc ctcccggcgg agcttttcgg ccaattgccc cgcggtccgg catccggacg
      901 cccattggag gaagatgggt ttcttccaga aaaaatccac gaaggcgccg cggcgcaagg
      961 gatatgtctg gaagaccccg aaggccatgg tcttgtcccg ggggcccgag ttcttttcca
     1021 cggcctcata ggccgaaaag gtgtcgaaat ccatgctgta gttctttttc aaacggtcca
     1081 tcggatcctg gaaacccaaa gcgtacccat agggccgggt cacccgggac tgcgccgccc
     1141 aaaccgaggc caaggacacc aacaacccgg ccgcgaaaag ccaacgcacc ttccgggaag
     1201 acggggtcaa gacccgggcc caagccagtc ccgacaggaa ggtcatggcc aggacggccc
     1261 cggcggcatg ccgcaagctg gtgcaaaaaa aggtccaggc cacgaggaag acaagggaga
     1321 agatcatcaa ggcccgggct tcccggtccc gcctgagttg ccaccaaaaa gggacgccca
     1381 tcaagatcag gggcgccagc ggcgccccca cttggttccc ggcggtccaa aaatccaaga
     1441 gccaggtccg ggccgccccc agaaaactca acccagccgg ggatccggtg tccgcccaaa
     1501 gggcggattc caaacccggg tcatacccgg agggcccacc ccaccaagcg gcggccatgg
     1561 gataaaaagg attcccgttg gccaaagcgt tgcgcaacaa ccaaggcagg caaggcgcca
     1621 aaaggaacag ggtccatttc caatcccagg cgatctttcg ttgtccccac ccatacgcaa
     1681 gcaccagcga accacaagcc acccaagcaa aaagggccgt gtatttcacg cccaaggccg
     1741 cccccaggaa aaagaaggcc gccgcccatg ccgccggacg aatgccgggt ccccgaaaag
     1801 cggccaccaa agccaggcag gaggacagtt cgtaaaaggc cagcaacccc tcgtcatatc
     1861 cccatcccat caaggccaac caataggggc aagccgccac caaaaggaac aaaggccatt
     1921 cgggcagctt caacgaccgg gcgagggacc gccccaggag caagacgccg accggcacca
     1981 aaaagcccga ggcccgcgcc aacggctccc cgcccatgct ccaaaaacca gcccagatca
     2041 attccgcccc cttcggaatg tcccccgtgt ggttcaccca atgcaggggg acctgatgga
     2101 gccgggcgac ctctcgggcg aaacggaaat ggtccaatac ggcgtcccaa atgatcgggg
     2161 gggagaggaa ctcaaagagc caggggagca acgccgccgc cagcagggcc ttttgccagc
     2221 gccgccagtc cgaccacccg gtcccggacg tccaccgcag gcccgccggg atccctgccg
     2281 ccagcaaaag cgccaccagc ggcccttcca ataaatggtt cacccccaac ccgaaaacgg
     2341 ccagcgaaaa acaggccagg gacatccccc aagccagggc gccctgttcc tcccaaagcg
     2401 ttcttccgaa aagaaaggac cggaaacttt gcgcccaacc cgccagcccc aggaaagccc
     2461 cgccggcttc caggcaggcc gcagctttgg atgcccaagc cttccacatc caatccatcg
     2521 accaggaggc cgccgagggg tcggaccaga cgggcaggaa ggacggcgcc cccatgcccc
     2581 aaagacaggc cgccaggaac gccagggacg ccaaaaaagg gaccacgatc agcctttcac
     2641 ggccaccagg ccggggcggt ttggttgaca at
//
LOCUS       DASTJJ010000418         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_670220, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000418 DASTJJ010000000
VERSION     DASTJJ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670220"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFR02_05195"
     CDS             <1..410
                     /locus_tag="VFR02_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9869873.1"
                     /translation="PVRLLNDADMQGLAVISGKGLELVVTLGTGVGTALFRNGALMPH
                     LELAQHPIHKDKTYNEYVGDKVLKQKGKKKWNRRVERVLGTLQTLLQPDRIYIGGGNG
                     VHIDFKLPRHTRIVPNQAGILGGIALWKEGALG"
     gene            403..>1047
                     /locus_tag="VFR02_05200"
     CDS             403..>1047
                     /locus_tag="VFR02_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869874.1"
                     /translation="MARTAYYLLGAVLFTTAFLAFRAWELGRGQAPVVPGGSPLALHY
                     DFHFAPPFFHYDLDAGRIAALKADKDGVEDHRILGLTVADFDLGSQYQFEEEHSWMAS
                     DYTFWLKELTVRFGYKSIDVYVARRYPKTSCAFQITLSHENDHVNIHRNLYLKYADIL
                     RRTLADGGTLPDRAHPVTALSQDEGKAELEKRVAAVLDPVFQDFQKELAEQEGEL"
BASE COUNT          201 a          329 c          336 g          181 t
ORIGIN      
        1 aaccggtccg gctgttgaac gacgccgaca tgcagggcct ggcggtcatt tcgggcaagg
       61 gtttggaact ggtggtgacc ctggggaccg gggtggggac cgcccttttc cgcaatggcg
      121 cgctcatgcc ccacctggag ctggcccagc atcccatcca taaggacaag acctacaacg
      181 agtatgtggg ggacaaggtc ctcaaacaga agggcaaaaa gaaatggaac cgccgggtgg
      241 aacgggtcct ggggacgctc cagaccctcc tccagcccga ccggatctac atcgggggag
      301 ggaacggcgt ccatatcgac ttcaagctgc cgcgccacac gcggatcgtt cccaaccagg
      361 ccggcatcct gggcggcatc gcgctttgga aggagggggc ccttggctag gaccgcctat
      421 tacctcttgg gcgccgtcct cttcacgacg gcctttttgg ccttccgcgc ctgggaactg
      481 gggcggggac aggccccggt agtcccgggc ggttcgccct tggcgctgca ttacgatttc
      541 catttcgccc cgccgttctt ccactatgac ctggacgcgg ggcggatcgc cgccctcaag
      601 gccgacaagg atggggtcga ggaccaccgc atcctgggcc tgacggtggc cgatttcgac
      661 ctggggtccc agtaccagtt cgaggaggaa cattcctgga tggcctccga ctatacgttc
      721 tggctgaagg aattgacggt ccggttcgga tacaaatcga tcgacgtgta tgtggcgcgc
      781 cgttacccca agaccagttg cgccttccag atcaccctca gccatgagaa cgaccacgtg
      841 aacatccacc ggaacctcta cctcaaatac gcggacatcc tgcgccggac cctggcggac
      901 ggagggaccc taccggaccg ggcccatccg gtgaccgccc tttcgcagga cgaggggaag
      961 gccgagctcg agaaacgggt ggcggccgtc ctggacccgg tcttccagga cttccagaag
     1021 gagttggccg aacaggaggg ggaattg
//
LOCUS       DASTJJ010000419         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_791384, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000419 DASTJJ010000000
VERSION     DASTJJ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791384"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..561
                     /gene="rpsG"
                     /locus_tag="VFR02_05205"
     CDS             91..561
                     /gene="rpsG"
                     /locus_tag="VFR02_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9869875.1"
                     /translation="MPRRKVAAKRDILGDSRYNNKLVTKFINCMMYNGQKMVATRSFY
                     DALDLLGEKHGEGALNVFKTAIDNIKPVLEVKSRRVGGATYQVPVEIRPERRTSLAIR
                     WVLIAARARKEKTIAEKLAAELADAFKNQGTAIKKKEDTHKMAEANQAFAHYRW"
     gene            575..2656
                     /gene="fusA"
                     /locus_tag="VFR02_05210"
     CDS             575..2656
                     /gene="fusA"
                     /locus_tag="VFR02_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9869876.1"
                     /translation="MSTRQVPLAKTRNIGIMAHIDAGKTTLTERILFYTGRTHKIGEV
                     HDGAATMDWMPQEQERGITITSAATTCFWKTNRINIIDTPGHVDFTIEVERSLRVLDG
                     AIGVFGAVEGVEPQSETVWRQATNYGVPRIAFVNKMDRVGADFFAAIDQMRGRLGANA
                     HALTLPVGAEDKFDGIIHLVEMKRYRWLDDSLGAKFEIEEIPADLKETADKYRKELVE
                     AVAEFDDDVMQKYLDGKDITEEELKSCIRKGTIANKFNPVLCGSAFKNKGVQPLLDAV
                     LNYLPSPLDIPSVKGHVVDGDEELTRETSDDAPFSALAFKIMTDPHVGKLVYFRVYSG
                     TLSVGSYVYNSTKGNRERVGRLLQMHANKREEIKEVHAGDIAATVGIKTITTGDTLCV
                     EDNPIILERMVFPEPVISVAIEPKTKADQEKMGLALGKLAEEDPSFRVKSDEETGQTI
                     ISGMGELHLEIIVDRMMREFNVQANVGKPQVAYRETIRKAVEAEGKYIKQTGGRGNYG
                     HCYIKMEPLEAGKGIEFVDAIKGGSIPREFIPAVQKGIMDAKDNGVLAGYPVVDFKVT
                     LFDGSYHDVDSNEMAFKIAASMAFKDAMKSADPVILEPVMGVEVVTPDDFMGPVMGDL
                     QSKRGKIEGMELRGTAQIIRSKVPLSEMFGYSTNLRSMTQGRATYSMQFAHYEETPRT
                     VQQEIIAKVKG"
     gene            2704..>2844
                     /locus_tag="VFR02_05215"
     CDS             2704..>2844
                     /locus_tag="VFR02_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9869877.1"
                     /translation="MSKEKFVRNKPHVNIGTIGHVDHGKTTLTAAITTVLSKKGFAQA
                     KGY"
BASE COUNT          646 a          897 c          875 g          426 t
ORIGIN      
        1 gcaagcaggg ccgctccaag tacggcatga agcgcccgaa aaaggaagcc gtcaagaagt
       61 aaccgttcac tgagaacaag gagagactga atgcctcgtc gcaaagtagc cgccaagcgg
      121 gacatcctcg gggattcccg ctacaacaac aaactggtca cgaagttcat caactgcatg
      181 atgtacaacg gccagaagat ggtcgccacc cgcagcttct acgacgccct ggaccttttg
      241 ggcgaaaagc acggggaagg cgccctcaat gttttcaaaa cggccattga taacatcaag
      301 cccgtgctgg aagtgaagtc ccgccgggtc ggcggcgcca cctaccaggt gccggtggag
      361 atccgccccg agcgccgtac gtcgttggcc atccgctggg tgctcatcgc cgcccgcgcg
      421 cgtaaggaaa agaccatcgc cgagaagctg gccgccgagc tggccgacgc cttcaagaac
      481 cagggcacgg ccatcaagaa gaaggaagac acccataaga tggcggaagc caaccaggcc
      541 ttcgcccact accgctggta atccaggaga tcccgtgagc acccgacaag tccccctcgc
      601 caagacccga aacatcggca tcatggccca tatcgacgcc ggcaagacca ccttgaccga
      661 gcgcatcctg ttctacacgg gccgcaccca taagatcggg gaagtgcacg acggcgccgc
      721 caccatggac tggatgccgc aagagcagga acgcggcatc accatcacct cggccgccac
      781 cacttgtttc tggaagacca accgcatcaa catcatcgac accccgggcc acgtggactt
      841 caccatcgag gtcgagcgct ccctgcgcgt gctggacggc gccatcggcg tcttcggcgc
      901 ggtggagggc gtggagcccc agtccgagac ggtctggcgc caggccacca actacggcgt
      961 gccccgcatc gccttcgtga acaagatgga ccgcgtgggc gccgacttct tcgccgccat
     1021 cgaccagatg cgtggccggc tcggggccaa tgcccacgcc ctgaccctgc cggtgggggc
     1081 cgaggacaag ttcgacggca tcatccactt ggtcgagatg aagcgctacc gctggctgga
     1141 cgacagcctg ggcgccaagt tcgagatcga ggagatcccg gccgacctga aggaaacggc
     1201 cgacaagtac cgcaaggaac tggtcgaggc cgtggccgag ttcgacgacg acgtcatgca
     1261 gaagtacctg gacggcaagg acatcaccga ggaagagctc aaatcctgca tccgcaaggg
     1321 gaccatcgcc aacaagttca acccggtgct ttgcggttcg gccttcaaga acaagggcgt
     1381 ccagcccctg ttggacgcgg tgttgaacta cctgccttcc cccttggaca tcccctcggt
     1441 gaaggggcat gtggtggacg gcgatgagga actcacccgg gagaccagcg atgacgcgcc
     1501 cttttccgcg ctggccttca agatcatgac cgatccccac gtgggcaagc tggtctactt
     1561 ccgcgtctat tcgggcaccc tttcggtcgg ctcctatgtc tacaactcga ccaaggggaa
     1621 ccgggaacgg gtcggccgcc tgctccagat gcacgccaac aagcgcgaag agatcaagga
     1681 agtgcacgcg ggcgacatcg cggccacggt gggcatcaag accatcacca cgggcgacac
     1741 gctctgcgtc gaggacaacc cgatcatcct ggaacggatg gtgttccccg agcccgtcat
     1801 ttcggtggcc attgaaccca agaccaaggc cgaccaggag aagatgggcc tggcgctcgg
     1861 caagctggcc gaagaggacc cctccttccg cgtcaagagc gacgaggaaa cgggccagac
     1921 catcatctcc gggatgggcg agctccacct ggagatcatc gtcgaccgca tgatgcggga
     1981 attcaacgtg caggccaacg tgggcaagcc ccaggtggcc taccgcgaga cgatccgcaa
     2041 ggccgtcgag gccgaaggca aatacatcaa gcagaccggc ggccgcggta attacggcca
     2101 ctgctacatc aagatggaac ccctcgaggc cggcaagggc atcgagttcg tggacgccat
     2161 caagggcggt tcgatccccc gggagttcat cccggcggtc caaaagggga tcatggacgc
     2221 caaggacaac ggggtcctgg ccggttaccc ggtggtggac ttcaaggtca ccctcttcga
     2281 cggttcctat cacgacgtcg actcgaacga aatggccttc aagatcgcgg cctccatggc
     2341 cttcaaggac gccatgaaga gcgccgatcc ggtcatcctg gaacccgtga tgggcgtgga
     2401 agtggtcacg ccggacgatt tcatgggccc ggtgatgggc gacctgcagt ccaaacgcgg
     2461 caagatcgag ggcatggagc tgcgaggcac cgcccagatc atccgctcca aggtgccgtt
     2521 gtccgagatg ttcggttatt cgaccaacct gcggtccatg acccagggcc gggccaccta
     2581 cagcatgcag ttcgcgcatt acgaggagac accgcggacg gtccaacagg agatcatcgc
     2641 gaaggtgaag ggctgatcca aggctgatca tccggccccg aggggccaag aggaggcgtg
     2701 tcaatgtcta aagagaaatt cgtgcggaac aagccccatg tgaacatcgg gacgatcggg
     2761 cacgtggacc acgggaagac gaccttgacg gcggcgatca cgacggtgct gtcgaagaag
     2821 ggcttcgcgc aggcgaaggg atac
//
LOCUS       DASTJJ010000420         1187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1404275, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000420 DASTJJ010000000
VERSION     DASTJJ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404275"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFR02_05220"
     CDS             complement(<1..835)
                     /locus_tag="VFR02_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9869878.1"
                     /translation="MFLQPTFWFYLFCQFQIFIFLVNRLGIRPHRQVKEFLWVLFFLT
                     AQGGATLALAGTWDFSAAAVAALPIPAKAWLALTFLWTAWLWWDHAWWLAVRKRPVDF
                     KPLRRGFPNRPSRWRAPFQALKGTFFENQIYDLEVVEYEVGLPGWPKAWDGLSLVQVS
                     DLHIGKFVHPTYLKFVVDQAKRLKPDLFALTGDFISFPHHLPLIGRLFKGLKAPLGVF
                     AVLGNHDHWSDAPGLVKVLEKDGVRVLVNEAVLFKRKGRTLALLGADDKWTGDKDTRP
                     LL"
     gene            complement(844..>1187)
                     /locus_tag="VFR02_05225"
     CDS             complement(844..>1187)
                     /locus_tag="VFR02_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9869879.1"
                     /translation="EADGETFETIGFKVMKRLKAKSVVLTRSEHGMTLFEAGLPPRTI
                     PTVAREVYDVSGAGDTVIATLTLALAAGATLLQAASLANYAAGIEVEKLGVATVSADE
                     LTQRLLEDLPR"
BASE COUNT          227 a          377 c          400 g          183 t
ORIGIN      
        1 cgagcaaggg acgcgtgtcc ttgtcccccg tccatttgtc gtcggcgccc agcagggcca
       61 gcgtcctgcc cttgcgtttg aagaggactg cctcattcac caggacccgc accccgtcct
      121 tctcgagcac cttcaccaag cccggcgcgt cggaccaatg gtcatggttc cccagcacgg
      181 cgaagacgcc caagggcgcc ttcaacccct tgaacaacct gccgatgagc ggcaagtggt
      241 ggggaaagga gatgaaatcg ccggtcaggg cgaagaggtc gggtttcaac cgcttggctt
      301 ggtccacgac gaattttaaa taggtgggat ggacgaactt accgatgtgg aggtccgaca
      361 cctggaccag ggacaggccg tcccaggcct tgggccaacc cggaagcccc acctcatact
      421 cgaccacttc caggtcatag atctggttct cgaaaaaggt ccctttcaag gcctggaagg
      481 gcgcccgcca gcgggaaggc cggttgggga aaccgcggcg cagcggcttg aagtcgacgg
      541 gccttttgcg gaccgccagc caccaggcgt ggtcccacca gagccacgcg gtccaaagga
      601 aggtgagggc cagccaggcc ttggcgggga tgggcagggc ggccacggcg gcggcggaaa
      661 agtcccaggt cccggccagg gccagcgtgg cgcccccctg ggccgtgagg aagaagagga
      721 cccagaggaa ttccttcacc tgccggtggg gccggatgcc caaccggttc accaggaaga
      781 tgaagatctg gaattggcaa aagaggtaga accagaacgt cggctggaga aacatggcgc
      841 cgctcagcgg ggcaggtctt ccaaaagccg ctgggtgagc tcgtcggcgg acaccgtggc
      901 cacgcccagt ttttccacct cgatgcccgc cgcgtagttg gccaacgagg ccgcctgcag
      961 cagcgtggcc ccggcggcca gcgccagggt gagggtggcg atgaccgtgt cgcccgcgcc
     1021 ggacacgtca tagacttccc gggccaccgt ggggatggtg cggggcggca ggcccgcctc
     1081 gaacagcgtc atgccgtgct cgctgcgggt gaggaccacc gatttggcct tgaggcgctt
     1141 catcaccttg aagccgatgg tctcaaaggt ctcgccgtcg gcctccg
//
LOCUS       DASTJJ010000421         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1527455, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000421 DASTJJ010000000
VERSION     DASTJJ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527455"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VFR02_05230"
     CDS             complement(<1..1500)
                     /locus_tag="VFR02_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9869880.1"
                     /translation="MIFPDGVERLVDFRLDPWPRWTYRLPDGTQIEQELFVRKGAPLT
                     ALRWTFKGPASKVTLSVRPLMSGRDYHSLHRENSFFKFSPVTHENQVEWKPYPGVPGI
                     VSYFNGAYFHEPQWFKNFAYTREKERGLDYTEDLASPGVFRFDLDRSEAVLVLTTSDQ
                     GGNLLDPVPANYYHKIAQEERARREAFPTRLHRAADDYLVRRDYGKTIMAGYPWFMDW
                     GRDAFIAIRGLCLAAGRLEEASFILLAWADKISQGLIPNRFPDQGEMPEYNSVDASLW
                     YVLAVADLLERRGDGMPQGNRIKLLDTVEAVLSHYAKGTRYGIQMDSDFLLQCGEPGT
                     QLTWMDAKANGVPVTPRVGKPVEVQALWLNALFALRERFPQWHDPAQEGLRNFQAKFW
                     NEDLGGLYDVVDVNHQKGRLDASLRPNQVLAVGGLPLCLLPPDKARRVVDQAESVLWT
                     PMGLRSLSPRDPAYQSTYKGNSVVRDTAYHQGTVWPWLAGPFVEAWVRVR"
BASE COUNT          271 a          501 c          493 g          252 t
ORIGIN      
        1 ccgcacgcgc acccaggctt ccacgaaggg gcccgccagc cagggccaga ccgttccctg
       61 gtgataggcc gtgtcccgga ccaccgaatt tcccttataa gtgctctgat aggccgggtc
      121 ccggggcgaa agggacctca atcccatggg ggtccaaagc accgactccg cctgatccac
      181 cacgcgcctg gccttgtccg gcggcaatag gcacaggggc agccccccca cggccagcac
      241 ctggttgggg cgcagggagg cgtccaggcg ccctttctgg tggttcacgt ccaccacatc
      301 gtaaaggccc cccaggtcct cattccagaa cttggcctgg aagttcctca ggccttcctg
      361 cgccgggtcg tgccattggg ggaacctttc ccgcaaggcg aaaagggcgt tgagccagag
      421 ggcctgcacc tccacgggtt ttcccacccg gggggtcacg ggcaccccgt tggccttggc
      481 gtccatccag gtcagctggg tgccgggttc cccgcactgc aacaggaaat ccgagtccat
      541 ctggatgccg tagcgggtgc ccttggcgta gtgggacagg acggcttcca ccgtgtccaa
      601 cagcttgatg cggttgccct ggggcatgcc gtcgccgcgc cgttccaaca ggtcggccac
      661 cgccaggacg taccagagcg aggcatccac cgaattgtac tcgggcattt cgccctggtc
      721 ggggaaccgg ttcgggatca ggccctggga gatcttgtcc gcccaggcca ggaggatgaa
      781 ggaagcctct tccaggcgcc cggccgccag gcacagcccc cggatggcga tgaaggcgtc
      841 ccgaccccag tccatgaacc agggataccc ggccatgatg gtcttgccgt aatcacgccg
      901 gaccaggtag tcgtccgccg cgcggtgcaa ccgcgtggga aaggcctccc gccgggcccg
      961 ctcctcttgg gcgatcttat ggtagtagtt ggcggggacc ggatccaaca ggttgccgcc
     1021 ctggtccgaa gtggtcaaca ccaggacggc ctcggaccgg tccagatcga agcggaagac
     1081 gcccggggag gccaagtcct cggtatagtc caatccccgc tccttctccc gggtgtaggc
     1141 gaagttcttg aaccactggg gctcgtgaaa ataggccccg ttgaaatagg acacgatgcc
     1201 gggcacgccc ggatagggct tccattccac ctggttctcg tgggtcacgg gggagaactt
     1261 gaagaagctg ttctcccggt ggagcgaatg gtaatcccgg cccgacatca ggggacgcac
     1321 ggaaagcgtc accttggatg ccgggccctt gaacgtccac cgcagggccg tgaggggggc
     1381 ccccttgcgc acgaagagct cctgctcgat ctgggtgccg tcgggaaggc ggtaggtcca
     1441 acgcggccag gggtccaacc ggaaatccac cagccgttcc acgccgtcgg ggaagatcac
     1501 atccgggtca tagcggt
//
LOCUS       DASTJJ010000422         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_548260, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000422 DASTJJ010000000
VERSION     DASTJJ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548260"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFR02_05235"
     CDS             <1..1227
                     /locus_tag="VFR02_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869881.1"
                     /translation="HLYGLVGELSNDSVKYHGLMAFLAPGFTVQADGDTLHTEGQNNL
                     SNNVYTNDRTRELFRTALDFGPFTLGAQVQPTQSNLVFKSHQFNLGNLVISGTGTIDT
                     LGGTSGLLINLAGAGHADQPHLRIGGVISTQLNPSQETDNLPVSNTLSSNNFIVTQTI
                     TDQNVLAWGPEIYFDVPNAFELALVGRFSNFDAFFEQNSTNTSSIGDIASFKAQSGNV
                     AVGIAVFKASSPLGAGLQLKTGGFFSIETADTSGFTPSGGANGGVSVTSWTVQLGTGF
                     EVPGDYTLGVQGYLQGVNGNEDNSGGVTSTNDFLDYKVQVGGERWLSKNWAMRLGVVY
                     EDGNNRGTAPENTFYFPIDPGMEVATTTITAGLGYQDPHLKMDLGLSAGQPSVADNPS
                     AYGDIYSVQLNAAVLF"
     gene            complement(1234..>1574)
                     /locus_tag="VFR02_05240"
     CDS             complement(1234..>1574)
                     /locus_tag="VFR02_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869882.1"
                     /translation="QQAASTNPQIMVGIYGNAVTFHPTTLLANSGIISYAMGVNTVNF
                     SPRVYLSPGIYWLALSPTALLPNIYFLTTGGTTYGYAGSTTALPSDLSLSASTGSIAA
                     AYQAQLDYCQ"
BASE COUNT          349 a          495 c          424 g          306 t
ORIGIN      
        1 cacctctatg ggctcgtggg cgaattgagc aacgactcgg tcaagtacca cggcttgatg
       61 gccttcctgg ccccgggctt caccgtccaa gccgacggcg acaccctgca caccgaaggc
      121 caaaacaatc tctccaacaa cgtttacacc aacgaccgga cccgtgaact tttccgcacc
      181 gctttggact ttggcccctt caccttgggc gcccaggtcc aaccgacgca gtccaacttg
      241 gttttcaaat cccatcagtt caatttaggc aatttggtga tctcagggac cggaaccatt
      301 gacacactgg gggggacatc cggccttttg atcaacctgg ccggggctgg acatgccgat
      361 caaccccact tgcgcatcgg cggggtcatt tccacccagc tcaaccccag ccaagaaacc
      421 gacaacctgc cggtcagcaa caccctgtcc tcgaacaact tcatcgtcac ccaaaccatc
      481 accgaccaga atgtcctggc ctggggaccg gaaatttact ttgatgtgcc caatgccttt
      541 gaactggccc tggtcggccg cttctccaat ttcgacgcct tcttcgaaca gaattccacc
      601 aacaccagtt ccatcggcga catcgccagt ttcaaagccc aatcgggcaa tgtggccgtg
      661 gggatagccg tcttcaaggc ttccagcccc ctaggagccg gcctccagct taagaccggc
      721 ggctttttct cgatcgaaac cgccgatacg tcgggcttca cgccttccgg gggggccaac
      781 ggcggggtga gcgtaacctc ctggacggtc caactgggga ccggctttga ggtccccggc
      841 gactataccc tgggcgtcca gggctatctg cagggggtca acggcaacga ggacaattcg
      901 ggcggggtga cctccaccaa cgatttcctc gattataagg tccaggtggg cggcgagcgt
      961 tggctttcca agaactgggc catgcgcctg ggggtggtct atgaggacgg caacaaccgg
     1021 ggcacggccc cggaaaacac cttctacttc cccatcgacc cgggcatgga agtggccacc
     1081 accaccatca ccgcggggtt gggttatcag gatccccacc tgaagatgga cctgggtcta
     1141 tccgccgggc agccctcggt ggcggacaac ccaagcgcct acggcgacat ctattcggtc
     1201 cagcttaacg cggccgttct cttttaaatc ccctcactga caataatcca actgagcttg
     1261 ataagcggcc gcaatagaac ctgttgatgc gctcaacgac aggtcagaag gaagggctgt
     1321 cgtgctcccg gcgtagccat aagtcgttcc cccggtcgtc agaaaataaa tgttcggcag
     1381 aagcgccgtt gggctcaggg ccaaccaata aattcccgga gaaagataaa cgcggggcga
     1441 aaagttgacc gtgttcacac ccatcgcgta agaaatgatc ccgctattgg ccaacaacgt
     1501 ggtcggatgg aaggtgacgg cattgccgta aatacccacc atgatctggg gattggtgga
     1561 agccgcctgt tgga
//
LOCUS       DASTJJ010000423         1385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1771608, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000423 DASTJJ010000000
VERSION     DASTJJ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771608"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFR02_05245"
     CDS             complement(<1..574)
                     /locus_tag="VFR02_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9869883.1"
                     /translation="MEKRKMKVLLAEDHPGGSPFWAKLLSEIKGTEIEFIHLQRLAEV
                     DQHIGREAPDVILLDLSLPGGAGLGTFLSVYGMAPEVPIIVITAADNPKLAVQTALEG
                     AQDCLVKGGLNGYLLIRAMRYAIGRQKHLKQLNEFLVIDELTGLYNRRGFLTLANQQL
                     KMSDRTGLPLLLVFADVDGLKAVNDSLGHHR"
     gene            complement(596..>1385)
                     /locus_tag="VFR02_05250"
     CDS             complement(596..>1385)
                     /locus_tag="VFR02_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HET9869884.1"
                     /translation="QEPIFLDTGLWLLIQPRTLSVGTMRTDLKRASTLHTVLEMTADP
                     VILFGPKPVTHPVEIPPLKKFQPGPGTFRATTNTRISYAEANAYFKDPRLHLIGMVFP
                     GTGDRKLTLEGVRLYGSGGQIIAEVKLHYNPPIINFTGKPARLTVYLRGTPRYLPKKR
                     MFDLPDLDYDIKSNDLLVEIADWIFKSDFKDQLRKIAVLPVGKKLDLIKEKVDRALNR
                     PLGPFARLRTQVTSFRVLDGFADNEGIEVRVAIQGNAALEVIWN"
BASE COUNT          260 a          426 c          424 g          275 t
ORIGIN      
        1 cccgatggtg ccccaaggaa tcattgacgg ccttcaggcc gtccacgtcc gcgaagacca
       61 gcagcagggg caacccggtc cggtcggaca ttttcagctg ttggttggcc agggtcaaga
      121 agccccgccg gttgtaaagg cccgtcaact cgtcgatcac caaaaattcg ttcagctgtt
      181 tcaggtgttt ttggcgccca atggcgtagc gcatggcgcg gatcagcagg taaccgttca
      241 ggccgccctt gaccaggcaa tcctgggcgc cctccagggc cgtttggacg gccaatttcg
      301 ggttatccgc cgccgtgatg acgatgatgg gcacctcggg cgccatgcca tagaccgaca
      361 ggaaggttcc caacccggcg ccgcccggca gggacaggtc cagcaggatg acgtcgggcg
      421 cctcccggcc gatatgttgg tcgacctcgg ccaaccgctg gagatggatg aactcgatct
      481 ccgtcccctt tatctcggac agcaacttcg cccaaaaggg ggagccgccc ggatggtctt
      541 ccgccaaaag gaccttcatt tttcgttttt ccaagggagc ccccccgccg gccggtcagt
      601 tccagatcac ctcaagggcc gcgttcccct ggatggcgac gcgcacctcg atgccctcgt
      661 tgtccgcgaa accgtccagg acccggaaag aggtcacttg ggtccggagc ctggcgaacg
      721 ggcccaaggg gcggttcagg gcccgatcga ccttttcctt gatcagatcc aattttttcc
      781 cgaccgggag caccgcgatc ttgcgcaatt ggtccttgaa atccgatttg aagatccaat
      841 cggcgatctc caccaagaga tcgttggatt tgatgtcgta gtccaggtcc ggcaggtcga
      901 acatccgttt tttcgggaga taacgcggcg tgcccctcaa ataaacggtc aagcgggccg
      961 gtttgccggt gaaattgatg atgggggggt tgtagtggag tttcacctcg gcgatgatct
     1021 gcccccccga tccgtataac cggacaccct ccagggtcaa tttccggtcg cccgtcccgg
     1081 gaaagaccat gccgatcaaa tgaagcctcg ggtccttgaa ataagcgttg gcttccgcgt
     1141 agctgatccg ggtgttggtc gtcgcccgga aggtgccggg ccccggctgg aacttcttca
     1201 gcggcgggat ctccaccgga tgggtcaccg gcttggggcc gaaaaggatg accggatcgg
     1261 ccgtcatttc caggaccgta tgcagcgtgg aagcccgctt caagtccgtg cgcatggtcc
     1321 ccacgctcag ggtccggggc tggatcagca gccacaggcc ggtatcgagg aagatgggct
     1381 cctgc
//
LOCUS       DASTJJ010000424         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_916945, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000424 DASTJJ010000000
VERSION     DASTJJ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916945"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VFR02_05255"
     CDS             <1..1337
                     /locus_tag="VFR02_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869885.1"
                     /translation="ETSQAQASSAAQYYAAGNQLYAAHNFAQAASYYSAATKVDPNYA
                     PAYQGLGNCYYALGRRQDSLAFYQRALALNPSNAQLSQFIQNLQSQLGASAPAAVATP
                     TAMQQGLALMKQRQYADSLGFFQQAIQDTPGDYQPYYYAGYASAMLRDNKNAALYFGL
                     ANQKHPNASIQNMAYRYKSALSADDQQWVSDQLGKSAQGQMVAGGSSKFTPNFGFHIN
                     GGAYNILSDPSLIEDGAKASGSVSLSGQTPSWSPMIGFQPYYQLSPNFEVNLLIAYLP
                     AGTLSYNWYDTAQGGTDDYQYTYNISMVNLGLGFRANFGSSSAVPYIEAGGEISPVSL
                     SFTKVPLNGTPSTSGFTDNFSGMAFGGHATLGVDFKLSKELLLGPYVGYRYLNVSSFT
                     NSSNEKLEVDANSGDVTTESQKAANGKAAGSYAPLTLDLSGIAAGVNLSLVF"
     gene            1414..>1831
                     /locus_tag="VFR02_05260"
     CDS             1414..>1831
                     /locus_tag="VFR02_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869886.1"
                     /translation="MTFRTIPWLFLALAGTLPAFAAGPALESQLGFYGDHEGFDGPFH
                     PAQDFLGAWVKTRLAAATGDSTGFEAGLYARDGDPGQTRVLPWLRFEYHTPTTRLLMG
                     SLESADRHGFLAPLEDPTLSFTRPVEYGLQWLEKDEG"
BASE COUNT          325 a          689 c          515 g          302 t
ORIGIN      
        1 ctgaaacgtc gcaggcccaa gcctcctccg cggcccaata ttacgccgcc ggcaaccagc
       61 tctacgcggc ccacaatttt gcccaggcgg cttcctacta cagcgcggcc accaaggtgg
      121 accccaacta cgccccggcc taccagggac tgggcaactg ctattacgcc ttgggccgcc
      181 gccaggattc cctggccttt taccagcggg cactggcctt gaacccctcc aacgcccagt
      241 tgtcccagtt catccagaac ctccaatccc aattgggcgc ctccgcgccc gccgcggtgg
      301 ccacccccac ggccatgcag cagggcctgg ccctcatgaa gcaacgccag tacgccgact
      361 cgctgggctt cttccagcag gccatccagg acacgccggg cgactaccag ccctattact
      421 acgcgggtta cgcctccgcc atgctgcgcg acaacaagaa cgccgcgctc tatttcggcc
      481 tggccaacca gaagcacccc aacgcgtcca tccagaacat ggcctaccgg tacaagtcgg
      541 ccctctcggc ggacgaccag caatgggtga gcgaccagtt gggcaagtcc gcccaagggc
      601 agatggtggc gggcggctcc tccaagttca cgccgaactt cggcttccac atcaacggcg
      661 gggcctacaa catcctgtcc gacccctcct tgatcgagga cggcgccaag gcctcgggct
      721 cggtgtccct gagcggccag accccctcct ggagccccat gatcggcttc cagccctatt
      781 accagctcag ccccaacttc gaggtcaacc tgctgatcgc ctacctgccg gccggcaccc
      841 tgtcctacaa ctggtatgac acggcccagg gcgggacgga cgactaccaa tacacctaca
      901 acatcagcat ggtgaacctg gggctgggtt tccgggccaa cttcggcagc tcgagcgccg
      961 tgccctatat cgaggccggc ggcgagatat cgccggtcag cctttccttc accaaggtgc
     1021 ccctgaacgg gaccccctcc accagcggct tcaccgacaa cttctcgggg atggccttcg
     1081 gcggccacgc caccctgggc gtggacttca agttgtccaa ggaactgctg ctgggcccct
     1141 acgtgggcta ccgctacctg aacgtcagca gcttcaccaa ttcatccaac gaaaagctgg
     1201 aagtggacgc caacagcggc gacgtcacca ccgaatccca gaaggccgcc aacggcaagg
     1261 ccgcgggttc ctacgcgccc ttgacgctcg acttgagcgg catcgccgcc ggcgtgaacc
     1321 tgtccctggt gttctgaccg tccccctccg ggggaaaagc tgcctgaacg ggggcgcctt
     1381 cgcccccgtt ttttattggc cccaaggaac cgcatgacct tccgcaccat cccctggctg
     1441 ttcctcgccc tggccgggac ccttcccgcc ttcgcggccg gccccgccct ggaaagccaa
     1501 ctggggttct atggcgacca tgagggcttc gacggcccct tccatccggc ccaggatttc
     1561 ctgggggcct gggtgaagac ccgcctggcc gcggccacgg gggattccac cggtttcgag
     1621 gcgggccttt acgcgcggga cggggacccg ggacagacgc gtgtcctgcc ctggttgcgg
     1681 ttcgagtacc acacccccac cacccggctc ctcatgggga gcctggaaag cgccgaccgc
     1741 cacggcttcc tcgcccccct ggaggatccg accctctcct tcacccggcc ggtcgagtac
     1801 ggcctgcagt ggctggaaaa ggacgagggt t
//
LOCUS       DASTJJ010000425         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_671705, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000425 DASTJJ010000000
VERSION     DASTJJ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671705"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFR02_05265"
     CDS             complement(<1..763)
                     /locus_tag="VFR02_05265"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HET9869887.1"
                     /translation="MAEKITVRLGDRSYPILIRSGSLGQAGARIREVSRAGQALILST
                     PPVYARYGGRLRRSLAGSFGRVSHCLVPDGEAHKNEKTLFSVFRRMQASGLHRDGCVV
                     ALGGGVVGDLAGLAASLYMRGTDFVQVPTTLLAQVDASMGGKTAIDFGGIKNLIGSFY
                     QPKMVLVDPDVLRTLDDRQLRTGLAEIIKYGVIRDADLFDRLEKGLSRFFAREPEFLA
                     LAIRRSAAIKADIISRDEREGGKRMWLNYGHTLGHA"
     gene            complement(793..1335)
                     /locus_tag="VFR02_05270"
     CDS             complement(793..1335)
                     /locus_tag="VFR02_05270"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9869888.1"
                     /translation="MSAKSNVSLIGFMGSGKTSSGQELARLLKFDFLDSDQLIQAESG
                     LTVREIFERYGEEGFRDRERAQIGRIQSLRRTVWATGGGAWLDPVLRESLQANSCTVW
                     LKVSGAQVWKRVSAHLAQRPLLAGAADPLAEIERRLAERRALYALADYTVETDHKTPR
                     EVAGEILGLIKRDGRLEPLD"
     gene            complement(1338..>1764)
                     /locus_tag="VFR02_05275"
     CDS             complement(1338..>1764)
                     /locus_tag="VFR02_05275"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9869889.1"
                     /translation="MSMQAVKGVSIGDGFGNAKAFGSQVHDEIGYRKGKGFFHWTNHA
                     GGLEGGMTNGEPVVVRVAKKPISTLKRPLRSVNIETKEVVKAAYERSDVCALPAASVI
                     GESICAWVFADALLEKLGGDFMGEVLDRYRLYLKHVRAY"
BASE COUNT          314 a          609 c          535 g          306 t
ORIGIN      
        1 acgcgtgccc cagggtatgg ccgtaattga gccacatgcg cttcccaccc tcgcgttcgt
       61 cccgggagat gatgtcggcc ttgatggcgg cggaccggcg gatggccaag gccaggaact
      121 ccggctcccg ggcgaaaaag cgggacagtc ccttttccaa gcgatcgaac aggtcggcgt
      181 cccggatgac gccatatttg atgatctcgg ccaagccggt ccgcagttgg cggtcgtcca
      241 atgtccgcaa aacatccgga tccaccagga ccatcttggg ttgataaaag gacccgatca
      301 agttcttgat cccgccgaaa tcgatggcgg tcttgccgcc catgctggcg tccacctggg
      361 ccagcagggt ggtcggcact tggacgaaat cggtgccccg catatacaac gaagctgcca
      421 gacccgccag gtcgcccacc acgccccccc ccagggccac cacgcaaccg tcccggtgga
      481 gccctgaggc ctgcatgcgg cggaagaccg aaaaaagggt cttttcattc ttgtgggcct
      541 ccccgtccgg gaccaggcaa tgggacaccc ggccgaaaga gccggccaag ctccggcgca
      601 gccggccgcc ataacgggcg tagaccggcg gggtggatag gatgagggcc tgcccggcgc
      661 gcgacacttc ccggatccgc gctcccgcct gtcccagcga tccggaacgg atgaggatcg
      721 ggtagcttcg gtcgcccaat cggacggtga tcttttcagc catgcgctct ctccgatggg
      781 tgggaagacg gctcaatcca ggggttccag ccgcccatcc cgcttgatca gccccagtat
      841 ctcccccgcg acttcccgcg gggtcttatg gtccgtctcc accgtgtaat ccgccaaggc
      901 gtaaagggcc cggcgttccg ccaggcggcg ctcgatctcc gccagggggt ccgccgcccc
      961 ggccaaaagc ggccgctggg ccaggtgggc cgagacccgt ttccaaacct gggcgccgga
     1021 gaccttcagc caaaccgtgc agctgttggc ctggaggctc tcccggagga ccgggtccaa
     1081 ccaagcgccg cccccggtcg cccacacggt ccgccgcagg gactggatcc ggccgatctg
     1141 ggccctttcc cggtcgcgga aaccctcttc cccgtagcgt tcgaagatct cccgtacggt
     1201 caggccggac tccgcctgga tgagctggtc cgaatccagg aaatcgaact tgagcagccg
     1261 ggccagttcc tggcccgagg aggttttccc cgaccccatg aacccgatga gcgagacgtt
     1321 gcttttcgcg gacatgccta gtaagcgcgg acgtgcttca ggtaaagccg gtatcggtcc
     1381 aggacctcac ccatgaaatc cccccccaat ttttccagga gggcgtcggc gaagacccag
     1441 gcgcagatgg attcaccgat caccgaggcc gccggcaggg cgcaaacgtc ggaccgttcg
     1501 taggccgcct tgaccacttc cttggtctcg atgttcaccg accgaagggg ccgtttcaag
     1561 gtggagatgg gttttttggc cacgcgcacc accaccggtt cgccgttggt catccctcct
     1621 tccaacccgc ccgcgtggtt ggtccaatgg aagaagccct tgcctttccg gtagccgatc
     1681 tcgtcgtgca cttgggaccc gaaagccttg gcgttcccga aaccgtcccc gatggagacc
     1741 cccttcacgg cctgcatgct catg
//
LOCUS       DASTJJ010000426         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1650587, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000426 DASTJJ010000000
VERSION     DASTJJ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650587"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFR02_05280"
     CDS             <1..736
                     /locus_tag="VFR02_05280"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HET9869890.1"
                     /translation="LVDEAEIAPKRKAAAKAPRAAKPAAKAPARAKKTASVPKPAASK
                     ARKAVRPKSPAKAARSKAPAKGSWKPAKYGETQVVAFIRDPNCIFVYWEIAAQKLEQV
                     QKELKGEYAKSRLVLRMFRSREDGQADLIYEIEVSPEDMNRYLPVHEPGRGYYVEVAR
                     KTASGKVVVLARSNALSTPTAGFSPLVDRAWTPAPALEEYLTEELEVGPDQLPAPGAL
                     GGVSSAEAQRRRRKFLDTFSSFGSPN"
     gene            809..>1252
                     /locus_tag="VFR02_05285"
     CDS             809..>1252
                     /locus_tag="VFR02_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HET9869891.1"
                     /translation="MSSPPGYLALVLHFHLPFVRHPEQENFLEEDWYYEALYETYLPL
                     LDRFERLAAQGTPFRLTVSLSPTLLALWADPLLRERVVRHGERLLALADREGERVRGQ
                     ADLEKVVAMYRGRLEAYHGAFTGRWAGNPLNGFKRFRDLGHLEIIT"
BASE COUNT          210 a          446 c          402 g          194 t
ORIGIN      
        1 gctggtcgac gaagccgaga tcgcccccaa acgcaaggcc gccgccaagg cgccccgggc
       61 cgccaagccc gccgccaaag cccccgccag ggcgaaaaag accgcttccg ttcccaagcc
      121 cgccgcctcc aaggcccgca aggccgtccg tcccaagtcc cccgccaagg ccgcccggtc
      181 caaggcgccg gccaaaggct cctggaagcc cgccaagtac ggcgagaccc aggtggtggc
      241 cttcatccgc gaccccaact gcatcttcgt ctattgggag atcgccgccc agaaactgga
      301 acaggtccag aaagaattga agggcgagta cgccaagagc cgcctggtgc tgcgcatgtt
      361 ccgcagccgg gaagatggcc aggcggacct catctatgag atcgaggtga gcccggagga
      421 catgaaccgt tacctgcccg tgcacgagcc gggccgtgga tattacgtgg aagtggcccg
      481 caagaccgcc tcgggcaagg tggtggtgct ggcccgttcc aacgccttga gcacgcccac
      541 ggcgggtttc tcgcccctgg tggaccgggc ctggaccccg gcgccggcgc tggaggagta
      601 cctgacggag gaactggaag tgggcccgga ccaattgccc gcgcccggcg ccctgggggg
      661 cgtctcttcc gccgaggccc aacgccgccg ccgcaaattc ctggacacct tctcgagctt
      721 cggcagcccg aattaagccg ggccgtcttg attttgcccc ggttcctttg tttacttggg
      781 gcgccatcct gactgaaaag ggtccgcctt gtcctcgccc cccggttacc tagcgctggt
      841 cctgcatttc cacctgccct tcgtgcgcca tcccgaacag gaaaatttcc tcgaggaaga
      901 ctggtattac gaggccctct acgagaccta cctgccgctg ttggaccgtt ttgagcggct
      961 ggcggcccag gggacgccct tccgcctgac ggtgtccctg tcgcccaccc tgctggccct
     1021 ctgggccgac cccctgctgc gcgagcgggt cgtccggcac ggcgaacgac tgctggccct
     1081 ggcggaccgc gaaggggaac gggtgcgcgg ccaggcggac ctggagaagg tggtggccat
     1141 gtaccggggg cgcctggagg cctaccacgg cgccttcacg ggccgttggg cgggcaaccc
     1201 gctcaacggg ttcaagcgct tccgcgacct gggccacctg gagatcatca cc
//
LOCUS       DASTJJ010000427         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_917632, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000427 DASTJJ010000000
VERSION     DASTJJ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917632"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="mrdA"
                     /locus_tag="VFR02_05290"
     CDS             <1..1181
                     /gene="mrdA"
                     /locus_tag="VFR02_05290"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9869892.1"
                     /translation="VQRRTLAYKNPVQGQTLMLSLDYKVQKLAEDLMTDNIGAVIVTK
                     PRTGEVLALVSKPNCDPNLFVSGISFKDWNRLIKDKTHPLENRAIQGQYPPGSIFKLI
                     TTMAALEDHVIDYDRVFLCRGIFWYKTWPYRCWRTSGHGWINLQRAIIESCDIYFYQL
                     GLLVKIDQISKVARMMGLGAKTGIDLDSEVTGLVPTPQWKESTQHQPWFPGNTIQMSI
                     GQGYLLTTPLQMLDMTSCIANNGTIYRPHLLYRVVDQKTGRAIYEQQPQVLTQAKFDP
                     DHLRFLQDTMERVISDSRGTGKKARIPGVRVAGKTGTSENPHGDNHAWFTAYAPADDP
                     QVAVIVLVENGGEGGIVSAPIAQRLMQAALGMEVTPWATPTVGPTPAEKPTPIMGVAP
                     "
     gene            1178..>1661
                     /locus_tag="VFR02_05295"
     CDS             1178..>1661
                     /locus_tag="VFR02_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9869893.1"
                     /translation="MKNYLKGLDWPLVIEVLLLTVIGLFVVYSATSRNLDISQLWKRQ
                     LVGFGIALLAMWLFSRIDYRFWIEASYIFYWLSIFSLVVVFFWGGSTNGAQRWIRLGI
                     LSFQPSELAKFSLILFLARYIGSRSPELFDLRRLLFLMVALGVPLLLILKQPDLGSAL
                     L"
BASE COUNT          295 a          572 c          500 g          294 t
ORIGIN      
        1 gcgtccagcg ccgcaccctg gcctataaga acccggtcca gggccagacc ctgatgctgt
       61 ccctggacta caaggtgcag aaactggccg aggacctgat gaccgacaac atcggggcgg
      121 tcatcgtgac caagccccgc acgggcgagg tgctggccct ggtgagcaag cccaactgcg
      181 accccaatct tttcgtcagc ggcatttcgt tcaaggactg gaaccgcttg atcaaggaca
      241 agacccatcc gctggagaac cgcgccatcc agggccagta cccgccgggc tccatcttca
      301 agctcatcac caccatggcg gccctggagg accacgtcat cgactacgac cgggtcttcc
      361 tctgccgcgg catcttctgg tacaagacct ggccctaccg ctgctggcgc acctcgggcc
      421 acggctggat caacctgcag cgcgccatca tcgagtcctg cgacatttat ttctaccaac
      481 tgggcctgct ggtcaagatc gaccagatct ccaaggtggc ccgcatgatg ggcctgggcg
      541 ccaagacggg catcgacctg gactcggagg tgaccggcct ggtgcccacc ccccagtgga
      601 aggaaagcac ccagcaccag ccctggttcc cgggcaacac catccagatg agcatcggcc
      661 agggctacct cttgaccacg cccctgcaga tgttggacat gacctcctgc atcgccaaca
      721 acggcaccat ctacagaccg cacctgctct accgcgtggt ggaccagaag acgggccggg
      781 ccatttatga gcagcagccc caggtgctca cccaggccaa gttcgacccc gaccacctga
      841 ggttcctgca ggacaccatg gaacgggtca tcagcgacag ccggggcacg ggcaagaagg
      901 cccgcatccc gggcgtgcgg gtggcgggca agacgggcac ctccgagaac ccgcacgggg
      961 acaaccacgc ctggttcacg gcctacgccc cggcggacga cccccaggtg gcggtcatcg
     1021 tgctggtgga gaacggcggg gaaggcggca tcgtgtccgc gcctatcgcc caacggttga
     1081 tgcaggccgc cctggggatg gaggtcacgc cctgggccac gcccaccgtg gggcccacgc
     1141 cggcggaaaa accgacgccg atcatggggg tggcgccttg aagaactacc tgaagggcct
     1201 ggactggccg ctggtcatcg aggtgctgct cttgaccgtc atcgggttgt tcgtggtcta
     1261 ttcggccacg tcgcgcaacc tcgacatctc ccagctgtgg aaacgccagc tggtcgggtt
     1321 cggcatcgcg ctgctggcca tgtggctctt ttccaggatc gactaccggt tctggatcga
     1381 ggcctcctac atcttctatt ggctgtcgat cttttccctg gtggtggtct tcttctgggg
     1441 cggttccacc aacggggccc agcgctggat ccgcctgggg atcctgtcct tccagccctc
     1501 cgaactggcc aagttctcgc tgatcctctt cctggcccgc tatatcggga gccgcagccc
     1561 cgaattgttc gacctgcggc gcctgctgtt cctgatggtg gccctggggg tgccgctcct
     1621 gctcatcctc aagcaacccg acctgggctc ggccctgttg c
//
LOCUS       DASTJJ010000428         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1405802, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000428 DASTJJ010000000
VERSION     DASTJJ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405802"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="pdxA"
                     /locus_tag="VFR02_05300"
     CDS             complement(<1..873)
                     /gene="pdxA"
                     /locus_tag="VFR02_05300"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET9869894.1"
                     /translation="MTQGDPAGIGGEVALKALRRPPGGASYLLVGGFDQLRTTAQKLK
                     VRLPLVRVREPQAVPGRVAVWDLGGPARVQWGKVSAANGAAAVRYIEEAARAALAGKV
                     QALVTAPINKEAIHRAGSKFPGHTEMLARISKTKKFAMMMVGGPFKITLQSIHLPLKD
                     AVGQVTKSRVLEKIAITQQALTRWFRVKRPRLAVAGLNPHAGENGAFGREELDVLKPA
                     VAAARAKGWDVTGPHPPDTLFYWAAQGRYDAVLCMYHDQGLIPFKLAAFDSGVNLTLG
                     LPFVRTSPDHGTAFD"
     gene            complement(1046..>2068)
                     /locus_tag="VFR02_05305"
     CDS             complement(1046..>2068)
                     /locus_tag="VFR02_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9869895.1"
                     /translation="IVGIALALKNRGENVRFISKDINVRIKADALGLIAEDFETNKIN
                     IEELYSGWTTITVKDAEVDEFYKTGKLALDAYLGAQSLYENQFLLLKSDIHEGKTALA
                     VYKESLKQAQPLWFKDPEAWGIKALNREQHFALNLLLNDRISMVTLVGSAGTGKTLLA
                     LAAGLLKSLDEKKYRRVLVSRPIVPMGKDIGYLPGTKDEKLMNWMQPIFDNLEFILDK
                     GHLQSDSEDVDDKVQYLLDSHKLELEALTYIRGRSIPKQFMIIDEAQNLTPHEIKTIV
                     SRAGVGTKIIMTGDPYQIDNPYLDSSSNGIVYLAERFKGQEIFGHITLSKSERSPLAA
                     LAAKTL"
BASE COUNT          330 a          637 c          677 g          424 t
ORIGIN      
        1 gtcgaaggcc gtgccgtggt cgggcgaggt gcgcacgaag ggcagcccca gggtcaggtt
       61 gaccccgctg tcaaaggcgg ccagcttgaa ggggatgagc ccctggtcat ggtacatgca
      121 gaggacggcg tcgtatcttc cctgggcggc ccaataaaaa agcgtgtcgg ggggatgggg
      181 ccccgtcacg tcccagccct tggcgcgggc cgcggccacg gctggcttca gcacgtccag
      241 ttcttcccgg ccgaaggcgc cgttctcccc cgcgtggggg ttcaacccgg ccacggccag
      301 gcggggcctt ttcacccgga accagcgggt cagggcctgt tgggtgatcg cgatcttttc
      361 caggacccgg gattttgtca cttggcccac ggcgtccttg agcggcaggt ggatggactg
      421 gagcgtgatc ttgaaggggc cgcccaccat catcatggcg aattttttcg ttttggagat
      481 gcgggccagc atctcggtat ggcccgggaa cttggaaccc gcccggtgga tggcttcctt
      541 attgatcggg gccgtgacga gggcctggac cttccccgcc agggcggccc gggcggcttc
      601 ctcgatatag cgcaccgccg ccgcgccgtt ggccgcggag accttccccc attggacccg
      661 ggcgggcccg cccaggtccc acaccgcgac ccggccgggg acggcttggg gctcccgcac
      721 ccggaccagg ggcaaacgga ccttgagttt ttgggcggtc gtccgcagtt ggtcaaatcc
      781 ccccaccagc aggtaggagg cgccccccgg gggacgacgc aaggccttca acgccacttc
      841 cccgccgatc ccggcgggat cgccttgcgt cacggcgatc aacggagggt cggttttgaa
      901 ttctgagttc ttggttctta gtgaaaacga acggtctgcc tgtttgatcg atcccaatgc
      961 cttgatttag cctcgcttac gggatttccg cgccggcgga aacatccaga actaagaact
     1021 caaaacctaa aactcatcac cggtttcaaa gggttttcgc ggccagggcg gccaggggcg
     1081 agcgctcgct cttggacagc gtgatgtggc cgaagatctc ctggcccttg aagcgttccg
     1141 ccaggtacac gatgccgttg gaggaggagt ccaggtaggg gttgtcgatc tggtaggggt
     1201 cgccggtcat gatgatcttg gtccccaccc ccgcccggga cacgatggtc ttgatctcgt
     1261 ggggggtcag gttctgggcc tcgtcgatga tcatgaactg cttggggatg gaccggcccc
     1321 ggatgtaggt gagggcctcc agctccagct tgtgggaatc cagcaggtac tgcaccttgt
     1381 cgtccacgtc ctcggagtcg ctctgcaggt ggcccttgtc caggatgaat tccaggttgt
     1441 cgaaaatggg ctgcatccag ttcatcagct tctcgtcctt ggtgcccggc aggtagccga
     1501 tgtccttgcc catgggcacg atgggccggg acaccagcac ccggcggtat tttttctcgt
     1561 ccaggctctt caggagcccg gcggccaggg ccagcagggt cttccccgtg cccgccgacc
     1621 ccaccagggt caccatggag atgcggtcgt tcagcagcag gttcagggcg aaatgctgtt
     1681 cgcggttgag cgccttgatg ccccaggcct ccgggtcctt gaaccaaagg ggttgggcct
     1741 gcttcaggct ttccttgtag accgccaagg cggtcttgcc ctcatggatg tcgctcttca
     1801 agagcaggaa ctggttctca tagagggact gggcgcccag gtaggcgtcc agggccagct
     1861 tgcccgtctt gtagaattcg tccacctcgg cgtccttcac cgtgatggtg gtccagcccg
     1921 agtaaagctc ttcgatgttg atcttgttgg tctcgaagtc ctcggcgatg agccccaggg
     1981 cgtccgcctt gatgcgcacg ttgatgtcct tggagatgaa ccgcacgttc tcgccgcggt
     2041 tcttcagggc cagggcgatc cccacgat
//
LOCUS       DASTJJ010000429         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_917787, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000429 DASTJJ010000000
VERSION     DASTJJ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917787"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFR02_05310"
     CDS             <1..419
                     /locus_tag="VFR02_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9869896.1"
                     /translation="LLAVRENEIAAEAMGVDTTAYKVKAFTASAAVAGVAGGLFAHYL
                     CYLNPSSFGFMKSVEIVIMVVLGGMGSVSGAVLAAFALTLLPEALRPLQAVTHVDLRM
                     VIYSLLLILIMILRPQGLFGRREIWQMVKYKRRKRE"
     gene            416..>1088
                     /locus_tag="VFR02_05315"
     CDS             416..>1088
                     /locus_tag="VFR02_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9869897.1"
                     /translation="MTPVLDVQNVTLAFGGLVAVDDLSLSLPEGGLVGLIGPNGAGKT
                     TVFNILSGIYRPQGGDVRVFGKPVLGLKPHQVTHAGLVRTFQNIRLFKELTVETNIQV
                     AFHHSRPYGIASALWGSRRFRYFEKAFAEETERLMEALELTHRRGALAGALPYGDQKK
                     LEIARALATGARILLLDEPAAGMNPQESQWLMEAIRRIRADFKLSLLLIEHDMRFVMG
                     LCERIH"
BASE COUNT          168 a          371 c          360 g          189 t
ORIGIN      
        1 ccctgctggc cgtgcgggag aacgagatcg ccgccgaggc catgggggtg gacaccacgg
       61 cctacaaggt gaaggccttc acggcctcgg cggccgtggc cggcgtggcg ggcgggctct
      121 tcgcccacta tctctgttac ctgaacccca gctccttcgg cttcatgaag tcggtggaga
      181 tcgtcatcat ggtggtgctg ggcggcatgg gctcggtgtc gggggccgtg ttggcggcct
      241 tcgccctgac gctcctgccc gaggccctgc ggccgctcca ggccgtcacc cacgtggacc
      301 tgcgcatggt gatctattcg ctcctgctca tcctcatcat gatcctcagg ccccagggcc
      361 tgttcgggcg ccgggaaatt tggcagatgg tgaaatataa gcgaaggaaa cgggaatgac
      421 gcccgtgctg gacgtgcaga acgtgacgct ggccttcggc gggctggtgg cggtggacga
      481 cctctcgctc tcgctccccg agggcgggct ggtgggcctc atcgggccca acggggcggg
      541 caagaccacg gtcttcaaca tcctgtccgg catctaccgg ccccagggag gcgacgtgcg
      601 ggtcttcggc aagccggtcc tgggcctgaa gccccaccag gtcacccacg cggggctggt
      661 gcgcaccttc cagaacatcc ggctcttcaa ggaactcacc gtcgagacca acatccaggt
      721 ggccttccac cattcccggc cctacggcat cgcctcggcc ctctggggtt cccggcgctt
      781 ccggtatttc gagaaggcct tcgccgagga gacggagcgg ttgatggagg ccctggaact
      841 gacccaccgg cgcggtgccc tggccggggc gctcccctac ggcgaccaga aaaagctgga
      901 gattgcccgg gccctggcga ccggcgcgcg catcctgctc ttggacgaac ccgccgccgg
      961 catgaacccc caggaatccc agtggctgat ggaggccatc cgccgcatcc gcgccgattt
     1021 caagctctcc ctcctgctca tcgagcacga catgcggttc gtgatgggcc tgtgcgaacg
     1081 gatccacg
//
LOCUS       DASTJJ010000430         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_672064, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000430 DASTJJ010000000
VERSION     DASTJJ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672064"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..797
                     /gene="bluB"
                     /locus_tag="VFR02_05320"
     CDS             129..797
                     /gene="bluB"
                     /locus_tag="VFR02_05320"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HET9869898.1"
                     /translation="MENHHAFSDAERSAVYRAIRERRAVRRFTKTPVPPEVLARLVQA
                     ALQAPSVGYSQPWNLILVSDPAVRLKVREAFGRANAEAQKLFQDERAEKYSALKLEGI
                     LDSALNICVTCDRNRFGPVVLGRTCQPDMDLYSAVCAVQNLWLAARCEGVGVGWVSIL
                     KPEELSAILGLPEGVVPVAYLCVGHTEAFAPEPELKTAQWLPEIPKSELVFYERWGGG
                     KPTP"
     gene            794..>1091
                     /locus_tag="VFR02_05325"
     CDS             794..>1091
                     /locus_tag="VFR02_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET9869899.1"
                     /translation="MKFPDIRWLALGWALAAPVWAGSYPQRVISMSPHFTEILYDIGA
                     QDQLVGVTDFCKYPPAARQKEKVGGFYNPSFEKIVALKPDLVLMVPFDPRSISGL"
BASE COUNT          187 a          378 c          342 g          184 t
ORIGIN      
        1 aaggcggacg gaggttcccg agccaggaga ccggccttga gcgacctgga tcagcttctt
       61 cgggaatggg aagcgccgtt tcgcccgccc ccgccgaagc gccagacctg acgcctcggg
      121 accgggccat ggaaaaccat cacgcctttt cggacgccga acgctcggcg gtctaccgcg
      181 ccatccgcga acgccgggcc gtacgccgct tcacgaaaac ccccgtcccg ccggaggtgc
      241 tggcccgcct ggtgcaggcc gccctccagg ccccctcggt gggatacagc caaccctgga
      301 acctcatcct ggtgtccgac ccggccgtgc gcctgaaggt ccgcgaggcc ttcgggcgcg
      361 ccaacgcgga ggcccaaaaa cttttccagg acgaacgggc cgaaaagtac tcggccctca
      421 agctggaagg catcctggat tcggccctca acatctgcgt gacctgcgac cgcaaccgat
      481 tcggcccggt ggtgttgggc cgcacctgcc agcccgacat ggacctctat agcgcggtgt
      541 gcgccgtgca gaacctctgg ctggccgccc gctgcgaggg cgtgggggtg ggttgggtga
      601 gcatcctcaa gcccgaggaa ttgtccgcca tcctgggcct gcccgagggc gtggtgccgg
      661 tggcctatct ctgcgtgggc cacacggagg ccttcgcgcc cgaacccgaa ttgaagaccg
      721 cccaatggct tcccgagatc cccaagtccg aactggtctt ttatgagcgt tgggggggcg
      781 ggaaaccgac cccatgaaat tcccggatat ccgatggctg gccctcggat gggccctggc
      841 cgcgccggtc tgggcgggaa gctaccccca acgggtcatt tccatgtccc cccacttcac
      901 cgagatcctc tatgacatcg gggcccagga ccaactggtg ggcgtgaccg atttttgcaa
      961 gtatcccccc gcggcccgcc aaaaggagaa ggtgggcggt ttctacaatc ccagtttcga
     1021 gaagatcgtg gccctgaagc ccgacctggt cctcatggtg cccttcgacc cccgttccat
     1081 ctcgggattg a
//
LOCUS       DASTJJ010000431         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1405910, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000431 DASTJJ010000000
VERSION     DASTJJ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405910"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1043)
                     /gene="bshC"
                     /locus_tag="VFR02_05330"
     CDS             complement(<1..>1043)
                     /gene="bshC"
                     /locus_tag="VFR02_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HET9869900.1"
                     /translation="YMGSEDADLEELGTVHIEGVTYRWQTAQQGAVGRMKPEGLAVLI
                     DAVTRPLGVGPFAREITALLKQAYLEHPDIQSATLFLVNALFGRYGLVVVNADTPGFK
                     RAILPVLTEELTGQTSHGIVSQTIGRLSSHYHPQATPREINLFYLEDQLRERILREGD
                     LWRVLNTSRTFTGPELEQELQEHPERFSPNVILRGLLQETILPDIAFIGGGGELAYWL
                     ELQDLFARYGVPYPVLMLRHSVLWVDKKSAQRLDKTGLAPAELFGDTEAVIAGFVKKH
                     TRHELVLKQEYEETEKLYAGLEEKASAIDLTLKASVAAERKRALCSIGKLEHKFLRAE
                     KKTFAWQAELIRS"
BASE COUNT          189 a          326 c          317 g          211 t
ORIGIN      
        1 gcttctgatc agttctgcct gccaggcgaa tgtctttttc tccgcccgca ggaatttatg
       61 ctccagcttg ccaatggagc aaagggcccg tttcctttcg gcggcaaccg aagctttcag
      121 ggtgagatcg atggccgacg ccttctcttc caggccggcg tagagcttct cggtctcttc
      181 gtattcctgc ttgagcacca gctcatgacg ggtgtgcttt tttacaaagc ccgcgatcac
      241 cgcctcggtg tccccgaaga gctccgcggg ggccaggccg gtcttgtcca ggcgctgggc
      301 cgatttttta tccacccaca gcaccgaatg acggagcatg agcaccgggt aaggcacccc
      361 atagcgggcg aacaggtcct ggagctccag ccagtaggcc agctctcccc ctccccctat
      421 aaacgctata tccggcagga tcgtctcttg cagcagcccc cggaggatga cattggggct
      481 gaaccgctcg ggatgctcct gcagctcctg ctccagctcg gggccggtga aggttcggga
      541 ggtgttgagc acccgccaaa gatcgccctc cctgaggata cgctcccgca actgatcttc
      601 caggtagaag aggttgatct cccggggggt cgcctgggga tgataatgcg aggacaaccg
      661 ccctatggtc tgtgagacaa taccgtggga ggtctggccg gtcagctctt cggtcaaaac
      721 cgggaggatg gcgcgtttga atcccggggt gtcggcgttg acgaccacca ggccgtagcg
      781 cccgaaaagg gcgttgacca gaaacagggt ggccgactgg atatccggat gctccagata
      841 ggcctgcttc agcaaggcgg tgatctcgcg ggcgaatgga ccgacgccga gcggacgggt
      901 aacggcgtcg atgaggacag ccagcccttc gggcttcatc cgccccacag cgccctgctg
      961 tgccgtctgc cagcggtagg tcaccccttc gatatgcacg gtgcccagct cctccaggtc
     1021 ggcgtcttca cttcccatgt aat
//
LOCUS       DASTJJ010000432         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1405925, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000432 DASTJJ010000000
VERSION     DASTJJ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405925"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFR02_05335"
     CDS             <1..202
                     /locus_tag="VFR02_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET9869901.1"
                     /translation="VDLGVKELRLLTNNPKKIVGLEGYGLKVVEQVPIEIAPNEVNAK
                     YLDTKRVKMGHVLKLTQEPPAK"
     gene            209..679
                     /gene="ribH"
                     /locus_tag="VFR02_05340"
     CDS             209..679
                     /gene="ribH"
                     /locus_tag="VFR02_05340"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009655927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9869902.1"
                     /translation="MPPKTIEGKPEGKGRRVAIVASRFNDLIGRRLVAGAVETLTFHG
                     AAEKDIEIAWVPGAWELPVAAARMLKTGRFDAAIALGTVIQGETTHHEYIAREVSASL
                     TRLTEATGLPVSFGVLTTENLDQALARAGESSNNKGAEAALHLLETLSVLERIK"
     gene            745..1146
                     /gene="nusB"
                     /locus_tag="VFR02_05345"
     CDS             745..1146
                     /gene="nusB"
                     /locus_tag="VFR02_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9869903.1"
                     /translation="MGLKRRTRELALQMLFQMDLGTVRLKDLEENFLPQASAPAGAKV
                     MALQMVRNTWDKLADIDQNLRALAENWDLARMAAVDRNILRLAAFEILYDAEVPRSVA
                     INEAIEIVKRFSTDESSKFVNGILDKLQRKN"
     gene            1178..1528
                     /locus_tag="VFR02_05350"
     CDS             1178..1528
                     /locus_tag="VFR02_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869904.1"
                     /translation="MGIDIPPDILAFIDGFIDAFITWDLLIYFARKGGEPTTLGQISQ
                     ILGRPEKEVAVPLKRLAEAGLVSAQKRVDGEALYRLSDSSKDFTAFKSFAAFNDQQEN
                     RLKILSYLLQKKVR"
BASE COUNT          315 a          488 c          516 g          260 t
ORIGIN      
        1 ggtggacctg ggcgtgaagg agctgcggct cttgaccaac aaccccaaga agatcgtggg
       61 gctggaaggc tacgggctga aggtggtgga acaggttccc atcgagatcg cgcccaatga
      121 agtgaacgcc aaatacctgg acaccaagcg ggtcaagatg ggccacgtgc tcaaactgac
      181 ccaggaaccg cccgcgaaat gagggaccat gccgcctaag accatcgagg ggaagccgga
      241 aggcaaggga cgccgggtgg ccatcgtggc cagccgcttc aacgacctca tcggccgccg
      301 gctggtggcg ggggccgtgg agaccctgac cttccacggg gccgccgaga aggacatcga
      361 gatcgcctgg gtgccgggcg cctgggagct gccggtggcg gccgcccgga tgctcaagac
      421 cggccgcttc gacgcggcca tcgccctggg gacggtcatc cagggcgaga ccacccacca
      481 tgagtacatc gcccgggaag tctcggcgtc cctcacccgt ctgaccgagg ccaccgggtt
      541 accggtttcc ttcggggttt tgaccaccga gaacctggac caggccctgg cccgggcggg
      601 ggagtcctcc aataataagg gcgcagaagc cgccctccac ctgctggaaa cgttatcggt
      661 gctggaacgg ataaaatagc tttgtgtttg cggggaagtt gtgtagtttg ttggctcttc
      721 ggctggacct gaacggggga aagcatggga ctcaaacgac gcacccgcga actggcgctc
      781 cagatgttgt tccagatgga cctgggcacg gtgcggttga aggacctgga ggagaatttc
      841 ctgccccagg ccagcgcccc cgcgggcgcc aaggtgatgg cgctgcagat ggtgcgcaac
      901 acttgggaca agctggcgga catcgaccag aacctgcggg ccttggccga gaactgggac
      961 ctggcgcgca tggcggcggt ggaccgcaac atcctgcgcc tggcggcctt cgagatcctt
     1021 tacgacgccg aggtgccccg gtcggtggcc atcaacgagg ccatcgagat cgtgaagcgt
     1081 ttcagcacgg acgagtccag caagttcgtc aacggcatcc tggacaagct gcagcggaag
     1141 aattgaattt atcggccagt gggtctatgg agcggctgtg ggaatagata taccgccgga
     1201 catcttagcc ttcatcgacg ggttcattga tgccttcatc acatgggacc tgctgatcta
     1261 tttcgcccgc aagggcggcg aacccaccac cctgggccag atctcccaga tcctgggccg
     1321 ccccgagaag gaagtggccg tccccctgaa gcgcctggcc gaggcgggcc tggtgtcggc
     1381 ccaaaagcgg gtggacggcg aggccctcta ccggctcagc gactccagca aggacttcac
     1441 ggccttcaag tccttcgcgg ccttcaacga ccagcaggag aaccgcctca agatcctcag
     1501 ctacctcctc caaaagaagg tccggtaacc cctttcgggg cccttggccc cgctgtcggg
     1561 ccttagtaca atgcctccg
//
LOCUS       DASTJJ010000433         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1161269, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000433 DASTJJ010000000
VERSION     DASTJJ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161269"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFR02_05355"
     CDS             complement(<1..449)
                     /locus_tag="VFR02_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HET9869905.1"
                     /translation="MTARPDTKRAYFTPALFAFLKKLKKNNNRPWFKAHQEDYERLVR
                     NPLLDFIGELGPSLRGVSPHLFVDNSPQGGSMFRIYRDTRFSKDKTPYKTHVAAWFPL
                     SRRKDVHTPGFYIHLAPGEVFVGGGIWHPDAPTLGVIRDYLARHPRE"
     gene            complement(446..646)
                     /locus_tag="VFR02_05360"
     CDS             complement(446..646)
                     /locus_tag="VFR02_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869906.1"
                     /translation="MSGFEFEPNDEVFHLKNHLIGLVLNVKEAEYPPGKRYVRVQIKP
                     DQPDQIWPVEEVGLWIRNKRFL"
     gene            complement(886..1536)
                     /locus_tag="VFR02_05365"
     CDS             complement(886..1536)
                     /locus_tag="VFR02_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9869907.1"
                     /translation="MKKTVSLLAVAGFLAASQAGAEEYGFDKAHTHIGFSVKHILSQV
                     PGEFKDYDGSFSFDPKDPSKDKIDVTIQADSISTDNEMRDHHLQSPDFFDVAKYPTIT
                     FKSRKVTAGTGDAYQVVGDLTMHGVTKPVTLSVTYNGSDKMMGADVVGFTATTTLDRR
                     DFGLTWGQDKLTAAGNLMVANDVAITLDVAGMDKDSLAKMAAMAKQKPAPAATPAK"
BASE COUNT          300 a          501 c          554 g          371 t
ORIGIN      
        1 cattcgcgcg ggtgccgggc caggtagtcg cggatgacgc ccagggtggg cgcgtccggg
       61 tgccagatgc cgccgcccac gaagacctcc ccgggcgcca ggtggatgta gaagcccggc
      121 gtgtgcacgt ccttccgccg ggacaggggg aaccaggccg ccacgtgggt cttgtagggc
      181 gtcttgtcct tggaaaagcg cgtgtcccgg tagatgcgga acatggaccc gccctggggg
      241 ctgttgtcca cgaagaggtg ggggctgacc ccgcgcaggg aagggcccag ctcgccgatg
      301 aagtccaaga gggggttgcg caccaggcgc tcatagtcct cctggtgggc cttgaaccag
      361 gggcggttgt tgttcttctt gagcttcttg aggaaggcga agagcgcggg cgtgaaatag
      421 gcccgcttcg tgtcggggcg ggccgtcata ggaaccgctt gttgcggatc cagaggccca
      481 cttcctccac gggccagatc tggtccggct ggtcgggctt gatctgcacc cgcacgtacc
      541 gcttgccggg cgggtattcg gcctccttca cgttcagcac caacccgatc aggtggttct
      601 tcaggtggaa gacctcgtcg ttgggttcga actcgaaacc gctcatggcc gcctcctgga
      661 cccgggatgc cccattttac cccaaaacaa aacggaatca ttcacctcgc caagtaaaga
      721 aaattggaac attgatagag gccgataagg cctgattttg atttccaaac gtcaggtttc
      781 atcagctttt atcattgttc aaggtttctt ttgcttgttt gtaaaagaca tggccccaaa
      841 acaaaaaaag gccggggcga aagccccagc cctcgggaaa gccttttact tcgcgggggt
      901 ggcggccggg gccggttttt gcttggccat ggcggccatc ttggccaggg agtccttgtc
      961 catgcccgcc acgtccaggg tgatggccac gtcgttggcc accatcaggt tgcccgcggc
     1021 ggtcagcttg tcctggcccc aggtcaagcc gaagtcccgg cggtccaggg tcgtggtggc
     1081 cgtgaaaccc accacgtcgg cgcccatcat cttgtcgctg ccgttgtagg tgaccgagag
     1141 ggtcacgggc ttggtcacgc cgtgcatggt caggtcaccc accacctggt aggcgtcccc
     1201 ggtcccggcg gtgaccttcc ggctcttgaa ggtgatcgtg gggtacttgg ccacgtcgaa
     1261 gaaatccggg gactgcaggt ggtggtcccg catctcattg tccgtgctga tgctgtcggc
     1321 ctggatggtc acgtcgatct tgtccttcga ggggtccttg gggtcgaagc tgaaggagcc
     1381 gtcgtagtcc ttgaactcgc ccgggacctg gctgaggatg tgcttcaccg agaagccgat
     1441 gtgggtatgg gccttgtcga acccgtattc ctccgcgccc gcctgggagg cggcgaggaa
     1501 tccggccacg gccagcagcg aaacggtctt tttcattttt gagtcctttc ttgatttaca
     1561 tccggttttg gatttatata gaccaagctc ttaccaaaag aaagtaggta ccattcggta
     1621 accgctctcc gcccggtggg gcttcccctt ttggacgccg cttggggcgg agaggggctg
     1681 gaaccgttcc ttacttagga gaaagaccat gatccgccat cgtccc
//
LOCUS       DASTJJ010000434         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1772880, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000434 DASTJJ010000000
VERSION     DASTJJ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772880"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..740)
                     /locus_tag="VFR02_05370"
     CDS             complement(300..740)
                     /locus_tag="VFR02_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9869908.1"
                     /translation="MADKAAEAAVVDETTARWVRAERAFTAVRLEGKSLRAVAEDLGV
                     SHETVWRDVRGYEQYLAAIQSQRDPKEMRAMVEALVLEGLQGCRDIIKANPTKPLIQV
                     GAWNTFASLLSHLRAVTGADVPKDRKPDNPGVVRVRWGGDDEAE"
     gene            complement(831..1043)
                     /locus_tag="VFR02_05375"
     CDS             complement(831..1043)
                     /locus_tag="VFR02_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869909.1"
                     /translation="MSDDTFTRRLDYYAALPRTAPLACRRLAFRAVCQALADTAEGLA
                     EAPATGRTNRDQARRVVRKARLMARL"
BASE COUNT          244 a          583 c          573 g          330 t
ORIGIN      
        1 agagcacctg gcttcggacc agggggttgt gggttcgact cctgccgagg gcgcctttac
       61 cgcatagaat caaggaaact ggaccagtca cgcggcccgg aatcccggag tagaccccct
      121 gtctactgcc gcccaggata gcggcgtggg ccgggccagt caagcgccgc gtcaagggcc
      181 gccccctggt ccccgcgccg ggctgtccgg ggcattccgg gccaggcatc cccccagggc
      241 gaacggacgg tgtgggcgat gctgtccggt atttccgggg ttccccggcc cctgctggtc
      301 tactcggctt cgtcatcccc gccccaccgg acccggacca cgcccggatt gtccggcttg
      361 cggtccttcg gcacgtcggc cccggtcacg gcccgcaggt gggacagcag gctggcgaac
      421 gtgttccagg cgccgacttg gattagcggc ttggtggggt tggccttgat gatgtcgcgg
      481 cagccctgca acccttcaag gaccagggct tccaccatcg cccgcatttc cttcgggtcg
      541 cgctgactct ggatggcggc caggtattgt tcgtagcccc gcacgtcgcg ccagaccgtt
      601 tcatgggaca cgcccaggtc ttcggccacg gcgcgcaggg acttcccttc caggcgcacg
      661 gccgtgaacg ccctctcggc ccggacccac ctggccgtcg tctcgtctac caccgcggcc
      721 tcggccgcct tgtctgccat gctagacccc cgccagtaac gcccggacgt gacagcccag
      781 gacgtgattc tcgtgattct acggcccaaa aaggcccggt tcgccctgcg ttacagccgc
      841 gccataagcc gggccttgcg gaccactcgc cgcgcctggt cccggttcgt gcgacccgtc
      901 gccggggctt cggccagccc ttcggccgtg tccgcgaggg cctgacagac ggcgcggaac
      961 gccagccttc ggcacgccag cggcgcggtc cggggcaggg ccgcgtagta gtccagtcgc
     1021 cgggtgaagg tgtcgtctga catggtgtct gtcctttcta gaatcgcggc ggggccgcga
     1081 ttgttggccc ggcccctggt tcgggacgcg ggattcgtcg tgactcggga cgaagattta
     1141 tgagaccaca gcatgagggc cgggggccgg gaatcctggc ccttgtggtc tctcgcgatg
     1201 cggtcgcgag tcacgaatca cgacgcctgg gccgggtgta gttcgtgacc cgcgactatc
     1261 gagaccaaca gaccacccag catgtagggg ccgggtttcc tgggccgggc tggtggccct
     1321 agtggtcttc gctgtcttct ggtctcgatt cgtcacgatg tcccggtctc gcgagtcgcg
     1381 accctagacc gctggatgaa gaccgcaggg gccgggttgc agggtggtct tcctgtctgt
     1441 ctcgacatcg cgacggtcgc gaatcgtgac cggccttctt gcggtcccga cagtacgtcc
     1501 cggtctcgat tcgtcgcgac tcgggggcca ggttcgcggt cgcgacctgg ggccggtctc
     1561 tagcacgggt acgcgagtga agtaataaga cggcgcaata ctggcgtcac gactgggttt
     1621 tcccgatgca gaacccggca aacttcggta ttttgcagcc ttttacccga actggggtag
     1681 gcattttgca gtacccttcc cgccccggcc gcgggttttt gcagtcctga
//
LOCUS       DASTJJ010000435         1271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1651360, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000435 DASTJJ010000000
VERSION     DASTJJ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651360"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1271)
                     /locus_tag="VFR02_05380"
     CDS             complement(<1..>1271)
                     /locus_tag="VFR02_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9869910.1"
                     /translation="RVDARGEVETLKDTINQMISNLRGTTQKNAEQDWLKTNLTRFTR
                     MLQGQRDLPTVAKAVLSELSQVVSAQHGAFYLASSKEPGETRLRLLSSFAYKPRPDLP
                     TEFALGEGLVGECAQEKARLLLGDVPDNYIRITSGLGEAAPVNIVVLPVLFEGQIKGV
                     LELASFAPFNTTHLAFLEQLMESIGIVINTLEASLRTETLLKHLQSQQEELQKTNQAL
                     EEKADQLALTSKYKSEFLSNMSHELRTPLNSMLILSQQLAEDSKSLTPKQVEYARTIH
                     ASGGDLLVLINDILDLSKVESGTMTLDMDVVPFSEMRDHMERLFRHVAEGKGLNFSVE
                     VDPRLGEDVHTDSKRLEQVLRNLLSNAFKFTEKGQVSLKVAPASGGWSPDHPVLGQAE
                     AALAFSVTDTGIGIPEDKQKIIFEAFQQADAG"
BASE COUNT          193 a          403 c          427 g          248 t
ORIGIN      
        1 ccggcgtctg cctgctggaa ggcctcgaag atgatcttct gcttgtcctc ggggatgccg
       61 atgcccgtgt cggtcaccga aaaggccagc gcggcctcgg cttggcccag caccggatgg
      121 tccgggctcc agcccccgga agccggggcg accttgaggg acacctggcc cttttcggtg
      181 aacttgaagg cgttggacaa caggttgcgc aatacctgct ccaggcgctt ggagtcggtg
      241 tggacgtcct cgcccaggcg ggggtccacc tccaccgaga aattgaggcc tttgccctcg
      301 gccacgtggc ggaacaggcg ctccatgtgg tcccgcatct cgctgaaggg caccacgtcc
      361 atgtccagcg tcatggtgcc cgactccacc ttggacaggt ccaggatgtc gttgatgagc
      421 accaacaggt ccccgccgga ggcgtggatg gtccgggcgt attccacctg cttgggcgtg
      481 aggctcttgg agtcctcggc caattgctgg gacaggatca acatgctgtt caggggcgtg
      541 cgcagctcgt gggacatgtt ggaaaggaac tcggacttgt acttggacgt gagggccagc
      601 tggtcggcct tctcctccag cgcctggttg gtcttttgca gctcctcctg ctggctctgc
      661 aggtgtttga gcagcgtctc ggtgcgcagg ctggcctcca gggtgttgat gacgatgccg
      721 atgctctcca tcaactgctc caggaaggcc aggtgggtgg tgttgaaggg cgcgaaggac
      781 gccagctcca gcacgccctt gatctgcccc tcgaacagca cgggcagcac cacgatgttg
      841 accggggcgg cctcccccag cccggaggtg atccggatat agttgtccgg cacgtcgccc
      901 agcagcagcc gggccttttc ctgggcgcat tcgcccacca ggccctcgcc cagcgcgaac
      961 tcggtgggca ggtccgggcg cggcttatag gcgaagctgg acaagagcct caggcgggtc
     1021 tcccccggct ccttggagga ggccaggtag aaggccccgt gctgggccga caccacctgg
     1081 ctcaactcgg acaggaccgc cttggccacc gtgggcaggt cgcgctgccc ctgcagcatg
     1141 cgggtgaaac gcgtcaggtt ggtcttgagc cagtcctgct cggcgttctt ctgggtggtg
     1201 ccccgcaggt tggagatcat ctggttgatg gtgtccttga gggtctccac ttcgccccgg
     1261 gcgtccaccc g
//
LOCUS       DASTJJ010000436         1462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_550077, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000436 DASTJJ010000000
VERSION     DASTJJ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550077"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFR02_05385"
     CDS             <1..738
                     /locus_tag="VFR02_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET9869911.1"
                     /translation="RQLAAADYLESDLEGYGTFKLAPKAWAVLKEGEKVFLREDPPAA
                     KGERGRRKAKSPIGAIRGDEGDPSLFQSLRALRSRLAKEQDLPPYVVFHDSTLREMAA
                     RRPATLAQFAQIPGVGEAKLKRYGPAFLEVLQAGKPPVPEAKAPKDARASAEETLDLF
                     RQGLDVPAIAARRGLTPGTVWEHLSGLIQEERLPAGEVVGLPDREMEKLRQALVEAQG
                     KLKPVFEAFGGKYSYDILKCVRSGIRG"
     gene            772..1188
                     /locus_tag="VFR02_05390"
     CDS             772..1188
                     /locus_tag="VFR02_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9869912.1"
                     /translation="MKQAKAGQGPSASQLISKRIADLGGWRGKTLARVRRLIQKADPA
                     VVEEWKWRGTPVWSHDGILCTGESYQKVVKLTFMKGASLKDPARLFNAGLEGNARRAI
                     DIHEGEELDAAAFQALIRQAVALNGSAPKKPAKKKA"
     gene            1267..>1462
                     /locus_tag="VFR02_05395"
     CDS             1267..>1462
                     /locus_tag="VFR02_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9869913.1"
                     /translation="MDPLTDVLTAISHPSRRAILSRLAQKGPARFLDIAEPFDTALNS
                     VTKHLKLLERAGLIERRKQGR"
BASE COUNT          288 a          511 c          455 g          208 t
ORIGIN      
        1 cgccaattgg ccgccgccga ctacctggaa agcgacctgg aaggctacgg caccttcaag
       61 ctggccccca aggcctgggc cgtgctcaag gaaggcgaaa aggtgttttt gcgcgaagac
      121 cccccggccg ccaagggcga acgcggccgc cgaaaggcca agtccccaat aggagctatc
      181 cggggggacg aaggcgatcc ttccctcttc caatccctgc gggccctgcg ctcccgcctg
      241 gccaaggagc aggacctgcc gccctacgtg gtcttccacg acagcacgct gcgcgaaatg
      301 gccgcccggc ggcccgccac cctggcccaa ttcgcccaga tccccggcgt gggcgaggcc
      361 aagctgaagc gctatggtcc cgccttcttg gaggtgttac aggccggaaa gccccccgtc
      421 cccgaagcca aggcgcccaa agacgcccgc gccagcgccg aggagaccct ggaccttttc
      481 cgccaaggcc tggacgtacc ggccatcgcc gcccgccggg gcctcacccc cggcaccgtc
      541 tgggagcact tgagcgggtt gatccaagaa gaacgcctgc cggccggcga ggtcgtcggc
      601 ctgccggacc gggagatgga aaagctcagg caggccctgg tggaagccca ggggaaattg
      661 aagccggtct ttgaggcctt tggggggaaa tattcctacg atatcctcaa atgcgtgagg
      721 agcgggatcc gcggttaacg cccgtgaggg cgccaagaaa ggaacgggac catgaaacaa
      781 gcgaaggcgg gccaaggccc atcggcttca cagctcatct cgaagaggat cgccgacctg
      841 ggcggttggc gcggcaagac cctggcgcgc gtgcgccgac tcatccaaaa ggccgacccg
      901 gccgtcgtcg aggaatggaa atggcggggc accccggtct ggtcgcacga cggcatcctc
      961 tgcaccggcg agtcctacca gaaggtcgtg aagctcacct tcatgaaggg cgcttccctg
     1021 aaggacccgg cccgtctctt caatgccggc ctggagggaa acgcccgccg cgccatcgac
     1081 attcacgagg gcgaggaact ggatgccgcc gccttccagg ccctcatccg ccaggccgtc
     1141 gccctcaacg gctccgcccc aaagaagccc gccaagaaaa aagcttaacg cgcccccctt
     1201 tcgggggccc caaaaccctt gaacaaaggg cttgcttttt attacacctt atggtgtaac
     1261 attggcatgg atccactgac cgacgtcttg accgccatct cccacccctc gcggcgcgcc
     1321 atcctcagcc ggctcgccca aaaggggccg gcccggttcc tggacatcgc cgagcccttc
     1381 gatacggcgc tcaactcggt gaccaagcac ctgaagctgc tggagcgggc ggggctcatc
     1441 gagcgccgca agcagggccg gg
//
LOCUS       DASTJJ010000437         1372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1284005, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000437 DASTJJ010000000
VERSION     DASTJJ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284005"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..>1372)
                     /locus_tag="VFR02_05400"
     CDS             complement(158..>1372)
                     /locus_tag="VFR02_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9869914.1"
                     /translation="DYYHALLAGNYIGMHAAVMYRREIFSRYLYDETLRACEDYDLYL
                     RMARDHPVFAHGEKLAAYRRHGSNMSGNLWLMKRQADRVLKKAADRDGRPEIRVLCRR
                     GRKNWRTFYAGQAFQRLKFGNRRPGQLPARDRLLALTSPEMLGPIAGRGLRKILRLFL
                     QILFLLREQAKRLTRDSLRYRIPRPGHVSAAHLRRTTPLSTRFGHDRGGPVDRWYIEN
                     FLAENAPAIRGRVLESGGNACTLAYGGDRVTGSDVLHVDPAHPGATFSCGLGRADAIP
                     SGIFDCIILTQALHLIYDLQAALDHCHRILKPGGTLLMTVPGISQLDHGEWKDSWYWS
                     FTPAALRKMLAPRFGAAPVIRSHGNVLAATAFLYGMGAGELSEKEKRETDPHYPLVVT
                     AAAIKPLVPCVP"
BASE COUNT          252 a          415 c          440 g          265 t
ORIGIN      
        1 cgcgcatgcc gctgcgcaag gacactttcc accgcgtcag ccaggtaccc ggcgtgattg
       61 taggtggtga tgatcaccga caccaggggc gcggccgggt tcgtttcccc tgccgggctg
      121 cgctgcaggt tcctttctcg cttttcggct gagggtatca tgggacacat gggacaaggg
      181 gtttaatggc tgcggccgtg acgacaagcg ggtaatgggg atcagtctcc cttttttctt
      241 tttctgacaa ttccccggca cccatgccgt agaggaaagc ggtggcggca agtacgtttc
      301 catgggagcg gatcaccggg gccgccccga aacggggtgc cagcattttg cgtaaggccg
      361 caggggtaaa ggaccagtac cagctgtctt tccattcgcc atgatccagc tgggaaattc
      421 cgggaaccgt catcagcagg gttccgccag gcttgagtat cctgtggcaa tggtccaggg
      481 ctgcctggag gtcatagatg aggtgcaggg cctgggtcag gatgatgcag tcgaagatgc
      541 ctgaagggat ggcatcggcc cggcccaggc cgcagctgaa ggtggcaccc gggtgggcgg
      601 gatcgacatg cagcacatcg ctgcctgtga ccctgtcccc gccataggcg agggtacagg
      661 cattaccgcc gctctcgagc acccgccccc tgatggccgg cgcgttttcg gccaggaaat
      721 tttcgatata ccagcggtcg accggcccac cgcggtcatg gccgaaacgg gtgcttaaag
      781 gagtcgtgcg cctcaggtgg gcagcgctca cgtgacccgg ccggggaatc cggtaacgca
      841 ggctgtcacg ggtgagccgc ttggcctgtt cccgcagcag gaacaggatc tgcaaaaaga
      901 ggcgcaggat cttccgcaac cctcttcccg ctatgggccc gagcatttcg ggggaggtca
      961 aagccagcag ccggtcccgg gcaggaagct gccccgggcg ccggttcccg aacttgagcc
     1021 gctggaaggc ctggccagca tagaaggtcc tccagttctt tcttcccctg cggcagagga
     1081 cgcggatttc gggacggccg tcccggtccg ccgccttctt cagcacccgg tccgcctgcc
     1141 ttttcatcag ccaaaggtta ccggacatgt tcgagccgtg ccggcggtag gctgccagct
     1201 tctctccgtg ggcaaataca ggatggtccc gcgccatccg caggtacagg tcataatcct
     1261 cgcaggcacg cagggtttcg tcatacaggt agcggctgaa aatctcccgc cggtacatca
     1321 ctgctgcatg catcccaatg tagttgcctg caagcagggc atgataatag tc
//
LOCUS       DASTJJ010000438         1358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_61109, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000438 DASTJJ010000000
VERSION     DASTJJ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61109"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFR02_05405"
     CDS             <1..452
                     /locus_tag="VFR02_05405"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9869915.1"
                     /translation="MFVGEAPGADEDAQGLPFVGKAGQLLTKIIGSTQVFRRPDVFIA
                     NVLKCRPPGNRTPLPEEVDQCRPYLEEQIKTIQPKLIMALGASAAQALLKTKDAVGKL
                     RGRWHDYGGIPLRVTYHPAALLRFEGYKKDVWEDMKEFTAKYLEIPE"
     gene            571..>1358
                     /locus_tag="VFR02_05410"
     CDS             571..>1358
                     /locus_tag="VFR02_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869916.1"
                     /translation="MKFGKLSTFFPALALFGLGLAPNLSAQCLVSDPFNGSLLSAWTT
                     LGIGGATGTTAVGAALTITSQDTGVAISGTTDSFTYLAQSVAGDPEITLEIDSMPASV
                     GGRAGLMLRASGTGNGNIYFFVGVNTTAGGASPSGNYFIQDRATTGGTSTALTTGGTW
                     TSAAPAYVRVTKIGTGFNGFYSSDGSSWTRIGGFTNIGGFPGGAYNAGIAMSSTVNAP
                     PGGSVAARNFNVLAGFCTPTSTPTATPTPTVTLTPTITLTSTKTP"
BASE COUNT          253 a          443 c          407 g          255 t
ORIGIN      
        1 tgatgttcgt gggcgaggcg cctggggccg atgaggacgc ccagggcctg cctttcgtgg
       61 gtaaggcggg gcagttgttg accaagatca tcggatccac ccaggtcttc cgccgcccgg
      121 acgtcttcat cgccaacgtg ctcaaatgcc gcccgccggg caaccgcacg cccttgcccg
      181 aggaagtgga tcaatgccgg ccctatttag aggagcagat caagaccatc cagcccaagt
      241 tgatcatggc cctgggggcc agcgcggccc aggccctgct gaagaccaag gacgcggtcg
      301 gaaagctgcg cggccggtgg catgactacg gcggcattcc cttgcgggtg acctaccatc
      361 cggcggccct gctgcgtttt gagggctaca agaaggacgt gtgggaagat atgaaggaat
      421 tcaccgccaa atacctcgaa atccctgaat aatcggcgaa agccctccgt aaagattctg
      481 ttaaaatgcc caatatccgg gggaatccgg gaaagcgcat gttaaactcg ggcatctttg
      541 tatgccaatc tcccgggcgg gtgcgctgaa atgaaattcg gcaaattatc gacctttttc
      601 cccgccttgg cccttttcgg gctggggctt gcccccaacc tttcggccca atgcctggtt
      661 tccgacccat tcaacgggtc ccttttgtcc gcttggacga ccctggggat cgggggcgcg
      721 accggaacca cggcggtggg cgcggccctg accattacca gccaggatac gggtgtcgcc
      781 atctccggga ccacggattc cttcacctac ctcgcccaat ctgtcgccgg ggaccccgaa
      841 atcaccttgg agatcgattc catgccggcc tccgtcggcg gccgggccgg cttgatgctg
      901 cgtgcctcgg ggaccgggaa cggcaacatc tactttttcg tgggtgtgaa caccaccgcc
      961 gggggggcct cgccttccgg gaactatttc atccaggatc gggcgaccac aggggggact
     1021 tcgaccgccc tgacgacggg ggggacctgg acctccgccg cgccggccta tgtccgggtg
     1081 acgaagatcg ggacgggctt caatgggttc tattcctccg acgggtcttc ctggactcga
     1141 attggcggct ttaccaacat aggaggattc cctggggggg cctataacgc agggattgcc
     1201 atgtcctcga ccgtgaacgc cccacccggg ggaagcgtgg cggccaggaa tttcaacgtt
     1261 ctggccgggt tctgcacccc cacctccacc ccgaccgcaa cccccacccc cactgtcacc
     1321 ttgaccccca ccatcacact gacgtccacg aagacgcc
//
LOCUS       DASTJJ010000439         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1040151, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000439 DASTJJ010000000
VERSION     DASTJJ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040151"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFR02_05415"
     CDS             <1..371
                     /locus_tag="VFR02_05415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9869917.1"
                     /translation="CLLLLSVGAAPGAPAGAEDRVAAQIAEVLIKSLRKVDVICRVSK
                     DQYAIILPDTATNTCGIIAKRIFKFFKQILGANPSVYINLGASAYPENSTEPKGLHDK
                     ATELLGQARQAGPNKAVLSD"
     gene            590..1687
                     /gene="dprA"
                     /locus_tag="VFR02_05420"
     CDS             590..1687
                     /gene="dprA"
                     /locus_tag="VFR02_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9869918.1"
                     /translation="MDERQTLLALHLTGVVGPRRLSSLREAFPRLSDAFGAGEDRLAY
                     LPHWTTSVARKVLALGDPLAAGERELEKARQVGAELLVAGDGDFPRVLTTLIDPPFVL
                     WRKGTTLPEDERALAVIGCRRPTPYGRELAGRLAQDLARSGYTVVSGLARGIDSLAHQ
                     GALRAPQGRTLAFLGSGLNHLYPPENRKLAEQVAERGALFSEYPLDAKPLALHFPQRN
                     RLISGASRGVLVVEANKDSGSFITVDHALDQGKPIFAVPGPVNHPQSEGTHSLIQQGA
                     RLVVGVGDIFDELSDRRARRAFQSPAPAPSAEVTPEEKILLESMGEEPLHLDALSRAV
                     GVPSRRLNEILLVLEMKGLVAQAPGHCYFKL"
BASE COUNT          272 a          589 c          557 g          279 t
ORIGIN      
        1 tctgcctgct cctgctcagc gtgggggcgg ccccgggcgc gccggcgggg gccgaggacc
       61 gggtggccgc ccagatcgcc gaggtgctca tcaagagcct gcgcaaggtc gacgtcatct
      121 gccgggtgtc caaggaccag tacgccatca tcctgcccga cacggccacc aatacctgcg
      181 gcatcatcgc caagcggatc ttcaagttct tcaagcagat cctcggcgcc aacccgtcgg
      241 tctacatcaa cctgggggcg tcggcctatc cggagaactc caccgaaccc aagggcctgc
      301 acgacaaggc cacggagctc ctgggccaag cccgccaagc cggccccaac aaggccgtcc
      361 tctcggactg acgtccaacc cccgaaccag tccaccgcaa agacgcaaag gcgctagaaa
      421 agccgaagga atgttcggtc gaatgatcaa aaggtttttc gccttgaaac cgtccgcttt
      481 tcatcggacc ggtccgttct tggcgccttg gcgtctttgc ggtgaagccg gtttttcttt
      541 ttctctggtt tttatgattg cccactaaaa gtcgttgtat actctcgcca tggacgaacg
      601 gcaaaccctt ttggccctgc acctgacggg cgtggtgggc ccccggcgcc tgtcctccct
      661 gcgggaggcc ttcccgcggc tctcggacgc cttcggggcg ggggaggacc gcctggccta
      721 cctgccccac tggaccacct cggtggcccg caaggtcctg gccctggggg accccttggc
      781 ggcgggggag cgggaactgg agaaagcccg gcaagtgggg gccgaattgc tggtggccgg
      841 ggacggggat ttcccccggg tcctcacgac cttgatcgac ccgcccttcg tgctgtggcg
      901 caaggggacc acgctgccgg aggatgaaag ggccctggcg gtgatcggtt gccgccggcc
      961 caccccctat ggccgggaac tggcggggcg cctggcccag gacctggccc ggtcgggcta
     1021 tacggtggtc tcgggcctgg cgcggggcat cgattccctg gcccaccagg gggcgctgcg
     1081 ggccccccag ggccgcaccc tggcgttcct gggcagcggg ctcaaccacc tctatccgcc
     1141 cgagaaccgc aagttggccg aacaggtggc cgagcggggc gccctcttca gcgagtatcc
     1201 gctggacgcc aaacccctgg ccctgcattt tccccaacgc aaccggctca tcagcggcgc
     1261 ctcccggggc gtcctggtgg tggaggccaa caaggacagc ggttccttca tcacggtgga
     1321 ccacgccctg gaccagggca agcccatctt cgcggtcccc ggcccggtga accatcccca
     1381 gtccgagggc acgcactcgc tcatccagca gggcgcgcgc ttggtggtgg gggtggggga
     1441 catcttcgac gaactgagcg accggcgggc ccggcgcgcc ttccagtccc ccgcgccggc
     1501 gccttccgcc gaggtgaccc ccgaggaaaa aatattgttg gagagcatgg gcgaggaacc
     1561 cctgcacctg gacgccttga gccgggcggt gggcgttccg tcccggcgcc tcaacgagat
     1621 cctgctggtg ttggaaatga agggactggt ggcgcaggcg cccggccact gctacttcaa
     1681 gctctaggcc gcgcgcc
//
LOCUS       DASTJJ010000440         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_794029, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000440 DASTJJ010000000
VERSION     DASTJJ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794029"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFR02_05425"
     CDS             <1..742
                     /locus_tag="VFR02_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9869919.1"
                     /translation="LSLGRRLSRNWSIFGTYDYEYDSIYNVQPPFNDPNNPQYVPSTS
                     GATSSFTPRVVYDSRDNYFDPHTGWKHQLSVELAGGPLGANYNYLKIVEDTSHFMPLP
                     LDFTLGEHFRVGLAQAYPFLGYYSDLPIYDKFFAGGTDTVRGFNERTVGPLNGGNALF
                     VNNLELKHTLFGPLRGVVFLDGGNCWTDYISVDGKLQWGAGLGLRLTIPGTVMAIRLD
                     YGWPVATDLPASSAPPGGVLHFNLGDIF"
     gene            882..>1156
                     /locus_tag="VFR02_05430"
     CDS             882..>1156
                     /locus_tag="VFR02_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9869920.1"
                     /translation="MGVARGVFVGLASFFVSHPRLRILLTNDDGFDAPGLRALIGAFQ
                     GLGDLAVAAPDEQASASGHSISLHKPIKAKADPGLGVSPAFRISSTPA"
BASE COUNT          194 a          397 c          348 g          217 t
ORIGIN      
        1 cttgagcctg ggacgccgct tgagccgcaa ttggagcatc ttcggcacct atgactacga
       61 gtacgacagc atctataacg tccagccgcc cttcaacgac cccaacaacc cgcagtacgt
      121 gccgtccacc agcggcgcca ccagcagctt caccccgcgg gtggtctacg acagccggga
      181 caactatttc gacccccaca cgggttggaa gcaccagttg tccgtcgagc tggccggcgg
      241 ccccctgggc gccaattaca actacctgaa gatcgtggag gacaccagcc acttcatgcc
      301 gctgccgttg gacttcaccc tgggcgagca tttccgggtc gggttggccc aggcctatcc
      361 cttcctgggc tattactcgg acctgcccat ctacgacaag ttcttcgcgg gtggcacgga
      421 caccgtgcgc ggtttcaacg agcgcaccgt aggccccttg aacggcggga acgccctttt
      481 cgtgaacaac ctggagctca agcacacgct gttcggaccc ctgcgcggcg tggtcttctt
      541 ggacgggggc aactgctgga ccgactacat cagcgtggac ggcaagctgc aatggggggc
      601 cggactcggg cttcgcttga ccatcccggg cacggtcatg gccatccggc tggactacgg
      661 ctggcccgtc gccacggacc tgccggcctc ctcggccccg ccgggcggcg tgctgcactt
      721 caacctgggc gatatctttt aaggcgatct aaggcgcacg caaagacgct aagccgcaaa
      781 gaacgcataa gcttggggtt gtaaacctcg ggtggtttgt cgtgtcgttc ttggcggctt
      841 agcgtccgcc ccaacacggt gatcgggtaa cggggccgcc cgtgggcgtc gcgcgaggtg
      901 tttttgttgg tttggcctcc tttttcgtct ctcatccccg cctgaggatc ctgctcacca
      961 acgacgacgg tttcgacgcc ccgggcctgc gggcgctgat cggggccttc cagggcctgg
     1021 gcgacttggc cgtggcggcc cctgacgagc aggccagcgc cagcggccat tccatctcgc
     1081 tccacaaacc catcaaagcc aaggccgatc cgggactggg ggtctcgccg gccttccgca
     1141 tctccagcac cccggc
//
LOCUS       DASTJJ010000441         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1406658, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000441 DASTJJ010000000
VERSION     DASTJJ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406658"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..412)
                     /locus_tag="VFR02_05435"
     CDS             complement(86..412)
                     /locus_tag="VFR02_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9869921.1"
                     /translation="MSDIMNVTVEELPGDAVILDVREDYEWEAGHIDGALHIPLEQLP
                     ERLEGLDPDEDVNVICRTGGRSFRATTWLTENGYSAFNVVGGMGAWFEAGRPMTSENG
                     ETPRVL"
     gene            516..>1013
                     /locus_tag="VFR02_05440"
     CDS             516..>1013
                     /locus_tag="VFR02_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HET9869922.1"
                     /translation="MSLNKSPAPAAEAPLDHRDKSFLGQPKMLLNLFSVELWERFSFY
                     GMQGILAYYMYYSVTKGGLGIDEGTALGIVGAYGGGVYLSTILGAWVADRLMGSERVL
                     FYSALIIMAGHLSLALLPGAAGLATGLILVGVGSGGLKANATSLVGTLYAKDDDRRDA
                     GFSIFY"
BASE COUNT          178 a          323 c          312 g          200 t
ORIGIN      
        1 aaaggtgtag ggcaggcggg aggagccggt cgctgtgttc atcgggacat tctgttcatg
       61 gcttgcctgt cagtttcggc agatgtcaaa ggacgcgggg cgtttcgccg ttttcactgg
      121 tcatgggtcg cccagcctca aaccaggccc ccatgccgcc caccacgttg aaggcactgt
      181 agccgttttc ggtcagccag gtggtggccc tgaaggaccg cccgccggtg cggcagatga
      241 cgttcacgtc ctcatcggga tcaaggccct ccaggcgctc gggaagctgc tccaggggga
      301 tgtgcagggc accgtcaatg tgcccggcct cccactcata gtcttcgcgg acatccaaaa
      361 tcactgcgtc gccggggagt tcctccacgg tgacgttcat gatgtctgac atggtcgtta
      421 ctcctgaacc tagggggtgc aatgcgttta agcttaatgg tccccgacac gccgcaatgt
      481 gacgggagca cgtcacaaat gcaaaaattg ggcaagtgag cttaaacaag agcccggcac
      541 ccgctgccga ggcacccctg gaccaccgcg acaaatcgtt ccttggacag cccaaaatgc
      601 tgctcaacct cttcagcgtg gagctgtggg aacggttttc cttttacggc atgcagggca
      661 tcctcgccta ctacatgtac tactccgtca cgaagggcgg actgggcatt gacgagggca
      721 cggcgctggg cattgtgggc gcctacggcg gcggcgtcta cctctccacg atcctcgggg
      781 cttgggtcgc cgaccgcctc atgggatccg aacgcgtcct gttctacagc gccctcatca
      841 tcatggccgg gcacctgtcc ctggccctgc tgcccggcgc cgcggggctg gccacgggcc
      901 tgatcctcgt tggcgtgggc tccggcgggc tgaaggcaaa cgccacctcg ctggtgggga
      961 ccctgtacgc caaggatgac gaccgccgcg acgccggctt ctccatcttc tac
//
LOCUS       DASTJJ010000442         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1161808, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000442 DASTJJ010000000
VERSION     DASTJJ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161808"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFR02_05445"
     CDS             complement(<1..794)
                     /locus_tag="VFR02_05445"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9869923.1"
                     /translation="MSQQHPGAPAGAPVCPRHPERVSYVSCQRCGRPTCPDCQRSAAV
                     GIQCVDCVRETAKAMPVRRTIFGGAVRTGRPVVTLTMMAICVVAYVLDLVLPNSFVFQ
                     TFAYAPFLTESEPWRMLTSAFLHSTNIMHIAFNMYALWILGQALEPAFGRARFLAIYV
                     ISALAGSVGVLLMTPVDTTVVGASGAIFGLFGALFVVQRKRGGDLRSIVVLLVINAAL
                     GFFIPGIAWQAHLGGLIAGALCAAVVSYAPARNRSLIQWGGMAAVVL"
     gene            complement(906..1448)
                     /locus_tag="VFR02_05450"
     CDS             complement(906..1448)
                     /locus_tag="VFR02_05450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9869924.1"
                     /translation="MTATPTAKATISTSLGDIVVNLFGNHAPKTVKNFVGLATGEIEW
                     THPATNEKSNAPLYNGTIFHRIIKDFMIQGGDPLGRGVGGPGYQFDDEIHPELNFNEP
                     YMLAMANAGVQGGRGTNGSQFFITTIPTGWLQGKHTIFGEVVDAESKAVVDAIEGVAT
                     GPGDKPREDVVINSITVENV"
     gene            1560..>1867
                     /locus_tag="VFR02_05455"
     CDS             1560..>1867
                     /locus_tag="VFR02_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869925.1"
                     /translation="MKTDDTWRKNVKTVDRLTQGLEGSLNAGVDSARDWAGPRVESAK
                     EWTAPRVEAAWGWAAPRIEKGFENASPKVQGGIRKAARYTADGVATVTPRIQEGLEKL
                     A"
BASE COUNT          357 a          571 c          642 g          297 t
ORIGIN      
        1 agcacaacgg cggccatgcc gccccattgg atgagcgaac ggttcctggc cggtgcgtag
       61 gaaacgacgg cggcgcacag ggccccggcg atgaggccgc ccagatgggc ctgccaggcg
      121 atgccgggga tgaaaaaccc cagggccgcg ttgatgacca gcaggacaac gatggaccgc
      181 aggtccccgc cccgcttccg ctgcaccaca aacaaggcac caaacaggcc aaaaatggca
      241 ccggaggcac ccaccacggt ggtgtccacg ggagtcatca acaacacgcc gacggaacct
      301 gccagcgcgc tgatgacgta gatggccagg aaacgtgccc gcccaaacgc cggctccagc
      361 gcctggccca aaatccacag cgcatacatg ttgaaggcga tgtgcatgat gttggtggag
      421 tgcaggaacg ccgacgtgag catacgccag ggctcgctct ccgtcaggaa cggtgcgtag
      481 gcgaaggtct ggaacacgaa gctgttgggc aggaccaggt ccagcacata ggccacaacg
      541 cagatggcca tcatggtcag cgtcaccacg gggcgtccgg tccgcacggc cccgccaaag
      601 atcgtccggc ggaccggcat ggccttggcc gtttcccgta cgcagtccac gcactggatg
      661 ccgacggcgg cactgcgctg gcagtcgggg caggtgggcc gcccgcaccg ttggcagctc
      721 acataggaga cccgctcggg gtgccggggg cacactggtg caccggcagg ggcgcccggg
      781 tgctgctggc tcatggctgg atccttatac gcgggaagaa aaatctgtgg ccgccggcgc
      841 agcggccggg aggggcccaa cgggtcctcc cagcccaacc gccggcggcc ggatgatgcg
      901 ggtgcctaga cgttttccac cgtgatggag ttgatgacaa cgtcttcgcg cggcttgtcg
      961 ccggggcccg ttgccacgcc ttcgatggcg tcgaccacgg ccttggactc ggcgtcgacc
     1021 acttcaccga agatggtgtg cttgccctgg agccagccgg tgggaatggt ggtgatgaag
     1081 aactgtgaac cgttggtgcc gcggccgccc tggacgccgg cattggccat cgccagcatg
     1141 taaggctcgt tgaagttcag ctccgggtgg atttcgtcgt cgaactggta gccgggtccg
     1201 cccacgccac ggcccagggg atcgccgccc tggatcatga agtccttgat gatgcggtgg
     1261 aagatggtcc cgttgtacaa gggggcgttc gacttttcgt tggtggcggg gtgcgtccac
     1321 tcgatttccc ccgtggccag gccaacgaag ttcttgacag tcttgggggc gtggtttccg
     1381 aagaggttca ccacgatgtc gcccaggctg gtgctgatag ttgcttttgc ggttggggta
     1441 gcagtcatgg cgtcattctg ccatgcgaaa ctgtcaatta tccccatgca ccgatgcatt
     1501 ccgcgccggg cgaactgtcc ggggatagca cgcggggtct ctccgacgta ggctaacaaa
     1561 tgaagacgga cgacacctgg aggaaaaacg tgaagacagt ggaccgcttg acccagggcc
     1621 tggaaggctc gctgaacgcc ggagtggaca gtgcccgtga ctgggccggc ccgcgggtgg
     1681 aaagtgccaa ggagtggacc gccccccgtg ttgaggccgc gtggggatgg gcggcacccc
     1741 gcatcgaaaa aggctttgag aacgcctctc cgaaggtgca gggcggcatc cgcaaggcag
     1801 cccgttacac ggccgacggc gtggccaccg tgacgcccag gatccaggaa ggcctggaga
     1861 agctggc
//
LOCUS       DASTJJ010000443         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1773510, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000443 DASTJJ010000000
VERSION     DASTJJ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773510"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFR02_05460"
     CDS             complement(<1..949)
                     /locus_tag="VFR02_05460"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HET9869926.1"
                     /translation="MLAGLFEDEVGLAFRKGLKGLEKGAAAGRAARYYRQLWDGLAQR
                     QEFNRHAAVGDGLQDHWLERLLDQPNAFHRKAELADPAKIGPALREAYAAELDLFLGV
                     LRADWAKAFRAAAPGLPPLSGFKPLEPLEASPAARARRDLKEALLRSGRRGADLAPEV
                     AGHFRRNGLGLFGRYRAFRWDHASQSLRGVGAVDPIELSNLVGYDEQRRPLLDNVRAF
                     VAGRPANNALLYGERGTGKSSTVKALLNAHQEEGLRLVEVLSAHLMDFPAILSHLRGR
                     PEKFILFVDDLSFEENETAYKGLKAVLEGTLEAPPKNVIL"
BASE COUNT          147 a          353 c          358 g          149 t
ORIGIN      
        1 tgaggatgac gttcttgggc ggcgcctcca gggtgccttc cagcacggcc ttcaggccct
       61 tgtaggcggt ctcgttctcc tcgaaggaca ggtcgtccac gaagaggatg aacttctcgg
      121 ggcgcccccg caggtgggac aggatggccg ggaaatccat caagtgggcc gacaacacct
      181 ccaccagccg caggccctct tcttggtggg cgttcaagag cgccttcacg gtggaggact
      241 tgcccgtgcc ccgctcaccg tagagcagcg cgttgttggc gggccggccg gcgacgaagg
      301 cgcgcacgtt gtcgaggagc ggccggcgct gttcgtcgta gcccaccagg ttcgagagct
      361 cgatggggtc caccgccccc acgccccgga gggactggga cgcgtggtcc caacggaagg
      421 cccggtaacg cccgaaaagg cccaggccgt tccggcggaa atgacccgcg acctcgggcg
      481 ccaagtcggc cccgcgccgg cccgaacgca gcagggcttc cttgaggtcc cggcgcgccc
      541 gggccgccgg ggaggcctcc agcggctcca ggggcttgaa ccccgaaagc gggggcagcc
      601 cgggggccgc cgcccggaag gcctttgccc aatcggcgcg gagcacgccc aggaaaaggt
      661 ccagctcggc ggcgtaggcc tcccgcaggg cggggccgat cttggcgggg tcggccaatt
      721 cggccttgcg gtggaaggcg ttgggctggt ccaggagcct ctccagccaa tggtcctgca
      781 ggccgtcgcc cacggcggcg tggcggttga actcctgccg ctgggccagc ccgtcccaga
      841 gctggcggta atagcgggcc gcccggccgg ccgccgcgcc cttttccagg cccttgagcc
      901 ccttgcggaa ggccaagccc acctcatcct cgaacaggcc cgccaacatg ccgaaggcct
      961 tcatccccgg ccaggcgggg gccgttgttt ttttcggggc ggctttt
//
LOCUS       DASTJJ010000444         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_672948, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000444 DASTJJ010000000
VERSION     DASTJJ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672948"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFR02_05465"
     CDS             <1..616
                     /locus_tag="VFR02_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869927.1"
                     /translation="VLLQPSDPRADLVRKEFKDATDTLVKSNKEGTPTVLRIMKIPAA
                     DIPAGPGKLFGFERVGPGAWIREYFDSAYLLGAGVLDYVEKVDSPYGPVPSAQGGHML
                     RILGEVSVPAKTTWRLSVTTANYAILKLDGRRVLDLKPNGRVVSRVRTLTLGPGDHAV
                     DYLVYLRVGNEIPEIKIEGPGGSVRRLGDPPPPAAPSAFQLGRP"
     gene            638..>1113
                     /locus_tag="VFR02_05470"
     CDS             638..>1113
                     /locus_tag="VFR02_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9869928.1"
                     /translation="MSADRGKKKPVLSIIVPVYNEEKTVALILQTVHRLPLKGVRKDI
                     VVVDDGSADGTAAVLRKIRIPGVRVLSHGKNRGKGAAIRTAIPHTRGDFVIIQDADLE
                     YDPNDYAKLLEPLLQGRADVVYGSRFRGTHRAFMFWHSIGNKFLTLVTNLLYDTILT"
BASE COUNT          214 a          337 c          366 g          196 t
ORIGIN      
        1 ggtcctcctc caaccttccg acccgcgggc cgacctggtc cgcaaggaat tcaaggacgc
       61 cacggacacg ctggtcaaaa gtaacaagga aggaaccccg acggttttaa ggatcatgaa
      121 gatccccgcg gcggatatcc ccgcgggacc cggaaagttg ttcggatttg agcgggtggg
      181 accgggcgcc tggatccggg aatattttga ttccgcttat ttgctgggcg cgggggtcct
      241 ggactacgtg gaaaaggtgg attcgcccta tgggcccgtg ccgtcggccc aggggggaca
      301 catgttacgg atattgggcg aggtttcagt ccccgccaag accacctggc gcctgtccgt
      361 gaccacggcc aattacgcca tcctgaagct ggacgggcgc cgggtgctcg atctgaaacc
      421 gaacggacgg gtcgtcagcc gggtccggac cctgaccttg gggccggggg accatgcggt
      481 ggactacttg gtttatttgc gggtggggaa cgagatcccc gagatcaaga tcgaagggcc
      541 gggagggtcg gtccgccggc tgggggatcc gccgccgccc gccgctccct cggcgttcca
      601 gctgggccgg ccgtaaagca agaaccagga gggagtcatg tccgccgacc gcgggaaaaa
      661 aaaaccggtc ctgtcgatca ttgttccggt atacaacgag gaaaagaccg tcgccttgat
      721 cctccaaacc gttcaccgct tgcccctcaa gggggtccgc aaggacatcg tggtggtgga
      781 tgacgggtcg gcggacggca cggcggcggt cctgcgcaag atccggatcc cgggcgtgag
      841 ggtcttgtcc catgggaaga accggggcaa gggcgccgcg atccgtaccg ccatccccca
      901 tacccggggt gacttcgtga tcattcagga cgccgacctg gaatacgacc cgaacgatta
      961 cgcgaaactg ctggaacccc tgctgcaggg ccgggccgat gtggtctacg gttcccggtt
     1021 ccggggcacg caccgggcgt tcatgttctg gcactccatc ggcaacaaat tcctgacgtt
     1081 ggtcacgaac ctgctctatg acaccatcct gac
//
LOCUS       DASTJJ010000445         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_550443, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000445 DASTJJ010000000
VERSION     DASTJJ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550443"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VFR02_05475"
     CDS             complement(<1..1362)
                     /locus_tag="VFR02_05475"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET9869929.1"
                     /translation="MPTPAYGLHWFRRDLRLAGNPALRWSWKEHRGRVLGVFCFDAKF
                     LARSDFSHDRFGFFLKTLGELRKELRAAGGDLLVLDQGPREGFTRLWKALRQKGVEAP
                     ATLSFNRDYEPFARERDAAMTEMLTGWGARVHTERDHLILEPEELFKQAPGDFYQVYT
                     PFARKWSSLFRSPRFRERVTGQKAALKAALAGPKKEKLFHLTWDGLFGGGCPLTDHFD
                     RVAQANAKKLSVPLPPAGTAAALARLSAFKKKLSFYHKDRDIPSRDATSQMSLYFKNG
                     SLTPAMALAHLGLEDEAFAAPGGPPRYLMEVVWREFYYHILWHCPRVEKEAFLERYKD
                     LPWENRKDLFEAWKEGKTGYPLVDAGMRQLRKTGWMHNRVRMVVASFLTKDLLIDWRW
                     GEKYFMEKLLDGDLAPNNGGWQWAASTGCDPQPYFRVFNPRLQSERFDPEGLYIREHV
                     PELA"
     gene            1483..>2114
                     /locus_tag="VFR02_05480"
     CDS             1483..>2114
                     /locus_tag="VFR02_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869930.1"
                     /translation="MARTRRQKKRDFQRWKDAFREPSLSVLLILQVFFIFLVRPLVSL
                     DKIPGFVLDWFLFAIAFISVFVVSSGGWTRVATLVAFGLLVTTTEWDKLISPGPMVHF
                     LSMAVACLFTLTVTVIIGGSVFGVGRVTQHHIQGAIVVYLNIALIFVTIYAGLADLIP
                     HFFSHLSDDPQAQFDQLLYFSLGSLTTATLGDISPLNPMARSLANLESVTG"
BASE COUNT          348 a          679 c          675 g          412 t
ORIGIN      
        1 cgccaattcg ggcacatgct cccttatata gaggccctcg gggtcgaacc gctcgctctg
       61 cagccgggga ttgaagaccc ggaaataggg ctgggggtcg cagccggtgg aggcggccca
      121 ttgccagccg ccgttgttgg gggccaggtc cccgtccagc agcttttcca tgaaatactt
      181 ctcgccccag cgccaatcga tgagcaggtc cttcgttaaa aaggacgcca ccaccatccg
      241 cacccggttg tgcatccagc cggtcttccg caattgccgc atgcccgcgt ccaccagggg
      301 atagccggtc ttcccttcct tccaggcctc gaaaaggtcc ttgcggttct cccatggaag
      361 gtccttgtag cgttccagga aagcttcctt ctcgacccgg gggcagtgcc agaggatgtg
      421 gtaatagaat tcacgccaga ccacttccat caggtagcgg ggcggcccgc cgggggccgc
      481 gaaggcctcg tcctcaagcc ccaagtgggc cagggccatg gccggggtca gcgatccgtt
      541 cttgaaatag aggctcattt gggaggtggc gtcccgggag gggatgtccc ggtccttgtg
      601 ataaaaagac agtttcttct tgaaggcgga aagccgcgcc agggccgccg ccgtgccggc
      661 cgggggcagg ggcacggaaa gcttcttggc gttcgcctgg gccacccggt cgaaatgatc
      721 cgtcaggggg cagccgccgc cgaagagccc gtcccaggtg aggtggaaga gtttttcctt
      781 tttgggtccg gccagggcgg ccttgagcgc tgccttttgg cccgtgaccc tttcccggaa
      841 acggggggac cgaaagagcg aggaccattt ccgggcgaag ggggtgtaga cctggtagaa
      901 atccccgggg gcctgcttga agagctcctc cggttccagg atcaggtggt cccgttcggt
      961 gtgcacccgg gccccccagc cggtgagcat ttcggtcatg gccgcgtccc tttcccgcgc
     1021 gaagggctcg tagtcgcggt tgaaggaaag ggtggcgggg gcctcgacgc ccttttgccg
     1081 cagggccttc cagagccggg tgaagccttc ccggggcccc tggtccagga ccaacaggtc
     1141 gcccccggcg gcgcgcagct ccttcctcaa ttccccgagg gtctttagga aaaagccgaa
     1201 acggtcgtgg gaaaagtcgc ttcgggcgag gaacttggcg tcaaaacaga agacgcccaa
     1261 gacccggccc cggtgttcct tccagctcca gcgcagggcg gggttgccgg ccaagcgcag
     1321 gtcccggcgg aaccagtgga ggccataggc gggggtcggc atgggtcggc tccttggggg
     1381 gtggcggccg ccccatctta gccattgggc gccccggcgt ccaatatccc cccggggagg
     1441 cttctgctaa aatccatcca ggtattttta aggaaggcgg acatggcccg aacgcggcgt
     1501 caaaagaaaa gggacttcca acgctggaaa gacgcgttcc gggaacccag cctgagcgtg
     1561 ctgctgatct tgcaggtgtt cttcatcttc ctggtgcgcc ccctggtcag cctggataag
     1621 atccccggtt tcgtcctgga ctggttcctt ttcgccatcg ccttcatttc ggtcttcgtc
     1681 gtttccagcg gcggctggac ccgggtggcc accctggtgg ccttcggtct gctggtgacc
     1741 accaccgaat gggacaagct catttccccg gggcccatgg tccatttcct ttccatggcg
     1801 gtggcctgcc ttttcaccct gacggtgacg gtcatcatcg gggggtcggt gttcggcgtg
     1861 ggccgggtga cccagcacca tatccagggc gccatcgtgg tctacctgaa catcgccctc
     1921 atcttcgtga ccatttacgc cgggctggcg gacctgatcc cccacttctt ttcccacctt
     1981 tcggacgacc cccaggccca gttcgaccag ctgctgtatt tcagcctggg gtccctgacc
     2041 accgccaccc tgggggacat ctccccgctg aaccccatgg cccgcagcct ggccaacctg
     2101 gaatcggtga ccgg
//
LOCUS       DASTJJ010000446         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_61629, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000446 DASTJJ010000000
VERSION     DASTJJ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61629"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFR02_05485"
     CDS             <1..914
                     /locus_tag="VFR02_05485"
                     /inference="COORDINATES: protein motif:HMM:NF020497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869931.1"
                     /translation="ALKQAIEKAAGDSKPKEAPKAAEPAPAKDTSATSSITLGELYLK
                     QGHFDKAIEVFQELLAKDPQNLMIRQKLSDVMDKQQKEAALGGGASSKLKKNEFTQPP
                     DQKEDVITEEVKADPKRQAKPKKEDDSKFTSEDILQVMRRGGKDDAVVEEKKAPAPPP
                     AAAKDEKKPEAKPEPAPAPSGPALKPEQVDKFKDILAELGTIDGIQRCFLIGKDGINV
                     VSIGETSNNADLGKQAVAIFQSTHRSVSQLNQGKLQQVLVTADSGNILLVALDQLVLI
                     VLANDKINLGLLRITLDSVLKKMGKVI"
BASE COUNT          282 a          315 c          315 g          176 t
ORIGIN      
        1 ccgcgctcaa acaggccatc gaaaaggccg ccggcgattc caaacccaag gaagccccca
       61 aggcggccga acccgccccc gccaaggata catccgccac cagttcgatc accctggggg
      121 agctttacct gaaacagggt catttcgaca aggccatcga ggtcttccag gagctcctgg
      181 cgaaggaccc gcagaacctg atgatccgcc aaaagctttc cgacgtgatg gacaaacaac
      241 agaaggaagc ggccctgggc ggcggggcct cctccaagct gaaaaagaac gaattcaccc
      301 agcctccgga ccagaaggaa gacgtcatca cggaggaggt caaggccgat ccgaagcgcc
      361 aggccaagcc caaaaaagag gacgattcca aattcaccag tgaggacatc ctgcaggtga
      421 tgcgccgggg cggcaaggac gacgccgtgg tggaggagaa gaaggcacct gccccccccc
      481 cggcggccgc gaaggacgaa aagaaacccg aggcgaagcc cgagcccgcc ccggcgccgt
      541 ccgggccggc gctgaaaccg gagcaggtcg acaaatttaa ggacattttg gccgaattgg
      601 ggacgatcga cggcatccaa cggtgttttt tgatcggcaa ggacggaata aacgttgtct
      661 ccatcggcga aacaagtaat aatgcggacc taggcaagca agccgtcgcc atttttcaaa
      721 gcacccatcg ttccgttagc cagctcaacc agggaaagct ccagcaggta ttggtgaccg
      781 ccgattcggg gaacatcctt ttggtcgcct tggatcagct tgtcctgatc gtactggcca
      841 acgacaagat caacttgggc ctgttgagga tcaccttgga ctccgttttg aaaaaaatgg
      901 ggaaagtcat ttaggccggc ttttgaaggg ccgacttcgg ggaacgcacc agcatggatt
      961 tcaagaaaat attggaagag accctctcca acgtggaagg ctccatcggg gccgggttgg
     1021 tcggcatgga cgggatcgtg atcgaccaga tctcccataa cacttccttc gacatcaacg
     1081 tggtcgga
//
LOCUS       DASTJJ010000447         1227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_184628, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000447 DASTJJ010000000
VERSION     DASTJJ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184628"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..994
                     /locus_tag="VFR02_05490"
     CDS             95..994
                     /locus_tag="VFR02_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9869932.1"
                     /translation="MPVKTEWTTDTFGHTYQGRSLYRGRTPFQSMEIFENGTFGTLLF
                     LDGKIQISRGDENRYHQYLVSAPLLAHRDPKSICVIGGGDCFALEEAVKFRSLKRILM
                     VEIDKGVVDFCRKHYPEIEKVLKDGRVDLVYEDARKRLETTSETFDVLIIDLTEPHGP
                     SKMLYTREFYRTCQKKLNPGGIIAIHTDNYYLFPESFATIYKTFHSVFPNILTARVDM
                     PCFGMGWTYRMASAKPISLKMMEKNIQKMKRQGTTFDHFTPSLYLVKPTNEELKVLKT
                     RGRVSTDRRPFDKFEKMQGYVTK"
     gene            1041..>1227
                     /locus_tag="VFR02_05495"
     CDS             1041..>1227
                     /locus_tag="VFR02_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869933.1"
                     /translation="MKILTKSEGLDWLRTNGLPTDGSELRFFHKSYIAYEFHNPPLTT
                     PQIMLADGLIQEILQLEG"
BASE COUNT          273 a          386 c          324 g          244 t
ORIGIN      
        1 cccggaattc acaagcccgc ccgggccttt tgtgtataat cccccaaaat tcagcccccg
       61 ccccggcggc gcggcacttt ttcaggagct tcccatgccc gtcaagaccg aatggaccac
      121 cgacaccttc ggacatacct atcaaggccg gtccctctac cggggccgga cgcccttcca
      181 gtccatggaa attttcgaga acgggacctt cgggaccctg ctcttcctgg acggcaagat
      241 ccagatcagc cggggcgacg agaaccgcta ccaccagtac ctggtgtcgg cgccgctcct
      301 ggcccaccgg gaccccaaga gcatctgcgt gatcgggggc ggggactgtt tcgcgctgga
      361 ggaggcggtc aagttcaggt cgctcaagcg catcctgatg gtggagatcg acaagggcgt
      421 ggtggatttc tgccgcaagc attacccgga gatcgagaag gtcttgaagg acggacgcgt
      481 ggacctggtc tacgaggacg cccgcaagcg gctggagacc acgtccgaga ccttcgacgt
      541 gctcatcatc gacctcaccg agccccacgg gccttccaag atgctctaca cccgggagtt
      601 ctaccggacc tgccagaaga agctgaaccc gggcggcatc atcgccatcc acacggacaa
      661 ctactacctg ttccccgagt ccttcgccac catctacaag accttccact cggtcttccc
      721 caacatcctc accgcccggg tggacatgcc ctgcttcggc atgggttgga cctaccgcat
      781 ggcctcggcc aagcccatct cattgaagat gatggaaaag aacatccaaa agatgaagcg
      841 ccaagggacc accttcgacc acttcacccc gtcgctctac ctggtgaagc ccaccaacga
      901 ggaactcaag gtcctcaaga ccaggggccg ggtctccacc gaccggcggc ccttcgacaa
      961 gttcgagaag atgcagggct acgtcacgaa ataggttctt caaggactat ttcttctttg
     1021 ggttctggag ctcatctacc atgaaaatat tgaccaagtc cgaaggttta gattggttgc
     1081 ggacaaatgg tttgccgaca gatgggtccg aacttcggtt ttttcacaaa tcttatattg
     1141 cgtatgagtt tcacaacccc ccgttgacca ctcctcaaat tatgctggcg gatgggctta
     1201 tacaggaaat tcttcagttg gaaggga
//
LOCUS       DASTJJ010000448         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_918924, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000448 DASTJJ010000000
VERSION     DASTJJ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918924"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFR02_05500"
     CDS             <1..670
                     /locus_tag="VFR02_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9869934.1"
                     /translation="EYLAYLEIMRSLNLEIAGDFLVMAATLMHVKSKMLLPIEPEAEE
                     EVEDPRAELMRRLLEYKKFKDAAEQMQEMEKTRTRLFGRLVPEDMKQVGEEEHLEEVT
                     LFGLLAAFKDVLIHSQEDPTAELVRPEITVTQKINDVMDLLQTHKKVNFKPMLEACRT
                     KIEKIICLLALLELMKLKLAKAFQNKIFDDIVIYLVQSDAPGEAPRDEAPSDAPAPDP
                     ALEP"
     gene            689..>1357
                     /gene="scpB"
                     /locus_tag="VFR02_05505"
     CDS             689..>1357
                     /gene="scpB"
                     /locus_tag="VFR02_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9869935.1"
                     /translation="MSKPNLNQILESLLFVSEQPLSPKRAAEILDDHPMEDIEWAFLA
                     LQEEINAMGRSFQVVQVAEGFQLVTRPEFHSWAKELYKVISTTRLSKASLEALAIIAY
                     KQPVTRAEVEAIRGVEVSNLVQSLLQKKMIRILGRAETAGRPLLYGTTHEFLIHFGLK
                     DLSDLPKVSEIQELAGDRPSPGFLKEMEGELRRRETKLGLSAAPLEGGEPAPPEGGET
                     PAPEN"
BASE COUNT          280 a          442 c          435 g          200 t
ORIGIN      
        1 ggaatacctg gcctacctgg agatcatgcg gtccctcaac ctggagatcg cgggcgattt
       61 cctggtgatg gcggccaccc tcatgcacgt caagagcaag atgctgctgc ccatcgagcc
      121 cgaggccgag gaagaggtgg aggatccccg ggccgagctc atgcgccgcc tgctggaata
      181 caagaaattc aaggacgccg ccgagcagat gcaggagatg gaaaagaccc gcacccgcct
      241 gttcgggcgc ctggtgcccg aggacatgaa gcaggtgggc gaggaggagc acctggagga
      301 ggtcaccctc ttcgggctcc tggcggcctt caaggacgtg ctgatccatt cgcaggagga
      361 ccccacggcc gaactggtgc gccccgagat cacggtgacc cagaagatca acgacgtcat
      421 ggacctgctg cagacccaca agaaggtgaa cttcaagccc atgctggagg cctgccggac
      481 caagatcgag aagatcatct gcctgctggc cctgctggaa ctcatgaagc tcaagctggc
      541 caaggccttc cagaacaaga tctttgacga tatcgtgatc tatctggtac agtccgatgc
      601 cccgggcgag gcgccgcggg acgaagcgcc gtccgacgcg ccggcgcccg acccggcgct
      661 ggaaccctag acccatacag gaaagcccat gtccaaaccc aatctgaacc agatcctgga
      721 gagcctgctg ttcgtcagcg agcaacccct gtcgcccaag cgcgccgccg agatattgga
      781 cgaccacccc atggaggaca tcgagtgggc cttcctggcc ctccaggagg agatcaacgc
      841 catggggcgc agcttccagg tggtgcaggt ggccgagggc ttccaactgg tcacccggcc
      901 ggagttccac agctgggcca aggaactcta caaggtcatc tccaccaccc ggctttccaa
      961 ggcctccctg gaggccctgg ccatcatcgc ctacaagcag cccgtcaccc gggccgaggt
     1021 ggaggccatc cggggtgtgg aggtcagcaa cctggtgcag tcgctgctcc agaagaagat
     1081 gatccgcatc ctgggccggg ccgagaccgc cgggcggccg ctgctctacg gcaccaccca
     1141 tgagttcctg atccatttcg gcctgaagga cctgtcggac ctgcccaagg tgtccgagat
     1201 ccaggaactg gccggcgacc ggccgtcccc gggcttcttg aaggagatgg agggcgaatt
     1261 gaggcgtcgg gagaccaaac tgggcctgtc ggccgcgccc ctggagggcg gggaaccggc
     1321 ccccccggaa ggcggggaga cccccgcgcc cgagaac
//
LOCUS       DASTJJ010000449         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_919097, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000449 DASTJJ010000000
VERSION     DASTJJ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919097"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFR02_05510"
     CDS             <1..361
                     /locus_tag="VFR02_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869936.1"
                     /translation="FRALGREVALSQAPHRAAGMAHCFSAPSKCELTLGGKKVCGGAQ
                     AREGGVFLQQGVILLSVSREWGRAYPTGELQAMTGLNDGPGAPVTASQVEEAVTAAFD
                     GAAMRLTAPGISGRLEP"
     gene            358..>1055
                     /locus_tag="VFR02_05515"
     CDS             358..>1055
                     /locus_tag="VFR02_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9869937.1"
                     /translation="MKKFDALVVGGGPGGYTAASLLGRRGLSVALFEKDKLGGSCLQV
                     GCIPTKLLQTAAVRYFQLAREGRSWGLEADQVRLNFPVLVERVQKTVSVLERGVQALL
                     DAAKVQVVKGEAQVLGPGRVRCGGEEYEGRALFLALGSRPRALPGFPLADDVVTSDGL
                     LRRPRLPRSLLIVGAGVIGLEFACLFAQLGVKVRVGDVLPGILPNMEEDLAQALLNSV
                     RKLGVEVELGLPEV"
BASE COUNT          145 a          334 c          399 g          177 t
ORIGIN      
        1 cttccgggcc ttggggcggg aggtggcatt gtcccaggcg ccccaccggg ccgccggcat
       61 ggcccattgt ttttccgcgc cctccaaatg cgagctgacc ctgggcggca agaaggtctg
      121 cggcggggcg caggcccggg aaggcggcgt ttttttgcaa cagggggtca tcctgctttc
      181 cgtttcccgc gagtggggcc gggcttaccc gacgggggag ctccaggcca tgacgggctt
      241 gaacgacggc ccgggcgcgc cggtcacggc ttcccaagtg gaggaggccg tcacggcggc
      301 cttcgacgga gcggccatgc gcttgaccgc gcccggaatt tccgggaggc tggaaccatg
      361 aaaaaattcg acgcgttggt ggtcggcggc gggccgggcg gctacaccgc cgcgtcgctc
      421 ctggggcgcc ggggcttgtc ggtggcgctc tttgagaagg acaagctcgg cggctcctgc
      481 ctgcaggtgg gctgcatccc caccaaactg ctgcaaacgg cggcggtgcg ctacttccaa
      541 ctcgcgcgcg agggccgttc ctggggcctg gaagccgacc aggtgcgctt gaattttccc
      601 gtgctggtgg aaagggtcca aaagaccgtc tcggtgctgg agcggggcgt gcaggcgctc
      661 ttggacgcgg ccaaggtcca agtggtgaag ggggaggccc aagtcctcgg ccccggccgg
      721 gtgcggtgcg gcggggaaga atacgaaggc cgcgcgcttt tcctggccct ggggtcccgg
      781 ccccgcgccc tgcccgggtt ccccctggcc gacgacgtgg tcaccagcga cgggctgctc
      841 cgccgccccc gactgccccg gtcgctcctg atcgtgggcg cgggcgtgat cgggctggaa
      901 ttcgcctgtc tcttcgcaca attgggcgtg aaggtgcggg tgggggacgt gctgccgggc
      961 atcctgccga acatggaaga ggacctggcc caggcgctct tgaattcggt gcgcaagctc
     1021 ggcgtcgagg tggaactggg cctgcccgaa gtgaa
//
LOCUS       DASTJJ010000450         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_184999, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000450 DASTJJ010000000
VERSION     DASTJJ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184999"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="proS"
                     /locus_tag="VFR02_05520"
     CDS             complement(<1..1053)
                     /gene="proS"
                     /locus_tag="VFR02_05520"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9869938.1"
                     /translation="MRVSQALIPTVKETPQEAEIPSHKLMLRAGLMRKVASGVYEYLP
                     LGQRVILKVMAIIREEMEAAGAQEVLMPILTPAELWQETGRWQKYGKELMRVKDRHER
                     DFALGPTHEEVITDLVRRELKSYKQLPLNLFQIATKFRDEVRPRFGVMRSREFLMKDG
                     YSFDVNDEACDKTYWKMYEAYKRIFKRCGLLSSPVEADSGLIGGSFTHEFMVLADTGE
                     AAIALCGACGYAANMEKAVGKPSAHTAPAAGGAFQKVPTPGKHTVADVGALLKVPAQQ
                     FIKTLIFIADGKPVAVLVRGDHEVNEVKVQNFLGAGEMGLASAEKVVEVTKAPVGFAG
                     PVGLSGLRVLADAELKG"
     gene            complement(1246..>1681)
                     /locus_tag="VFR02_05525"
     CDS             complement(1246..>1681)
                     /locus_tag="VFR02_05525"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET9869939.1"
                     /translation="IGAVLSDGIGDTIRVSLTADPVEEVKVATHLLRSMGFRKEGVDI
                     VACPTCGRCHVDLEKMVDQVETATLTMKTPMRVAVMGCVVNGLGEGAEADLGICSEKG
                     HGVIIEKGEIKVRLKEEELVPHLIGEIKKRSAEMEKAAQKTS"
BASE COUNT          273 a          556 c          533 g          319 t
ORIGIN      
        1 gcccttcagt tcggcgtcgg ccagtacgcg caggcccgac agccccacgg gcccggcgaa
       61 ccccacgggc gccttggtca cctccaccac cttctcggcg gacgccaggc ccatctcgcc
      121 cgcgcccagg aagttctgca ccttgacctc gttgacctcg tggtcgcccc gcaccagcac
      181 cgccacgggc ttgccgtcgg cgatgaaaat gagggtcttg atgaactgtt gggcggggac
      241 cttaaggagc gcccccacgt cggccacggt gtgtttgccg ggggtgggga ccttctggaa
      301 cgccccgccc gccgcggggg cggtgtgggc cgagggcttg ccgacggcct tctccatgtt
      361 ggcggcgtag ccgcaggccc cgcagagcgc gatggcggcc tcgccggtgt cggccaggac
      421 catgaactcg tgggtgaagc tgccgccgat caggcccgaa tcggcctcca ccggggagct
      481 caggagcccg caacgcttga agatgcgctt gtaggcctcg tacatcttcc aataggtctt
      541 gtcgcaggcc tcgtcgttga cgtcgaagga atagccgtcc ttcatgagga actcgcggct
      601 gcgcatgacg ccgaagcggg gcctcacctc gtcgcggaac ttggtggcga tctggaagag
      661 gttcagcggc agctgcttgt agctcttgag ctcccggcgc accaggtcgg tgatgacctc
      721 ctcgtgggtg gggcccagcg cgaagtcccg ctcgtggcgg tccttcaccc gcatgagttc
      781 cttgccgtat ttctgccaac ggccggtctc ctgccagagt tcggcggggg tgaggatggg
      841 catgaggact tcctgggccc cggcggcctc catctcctcg cggatgatgg ccatgacctt
      901 gaggatgacc cgctgcccca ggggcaggta ctcatagacg cccgaggcga ccttccgcat
      961 cagcccggcg cgcagcatga gcttgtggga cgggatctcg gcctcttggg gcgtttcttt
     1021 gaccgtgggg atgagggctt gggacactcg cataaaagtc cttaatgatc aagattgaaa
     1081 ggcaaaacct gttccaccgc agagacgcgg agccgcggtc cacatacgga gaagatcatt
     1141 tgccttactg cgtctccgcg cctctgcggt gaaaaggatc tccaaataaa agaggcgggt
     1201 ggaaacccgc ctcaggaacg acactataac gccaaacccg cttctttacg aagtcttttg
     1261 ggcggccttt tccatctctg cgctgcgttt cttgatctcg ccgatcaggt ggggcaccag
     1321 ttcctcttcc ttcaaccgca ccttgatctc gcccttctcg atgatgacgc cgtggccctt
     1381 ctccgagcag atgcccaggt cggcctccgc gccctcaccc aggccgttga ccacgcagcc
     1441 catgacggcc acccgcatgg gggtcttcat ggtgagggtg gcggtctcca cctggtccac
     1501 catcttttcc aggtccacgt ggcagcgccc gcaggtgggg caggccacga tgtccacgcc
     1561 ttccttgcgg aagcccatgg accgaagcag gtgggtggcc accttcactt cctccaccgg
     1621 gtcggccgtg agggagacgc ggatggtgtc cccgatgccg tcggaaagca cggccccgat
     1681 g
//
LOCUS       DASTJJ010000451         1723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1407684, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000451 DASTJJ010000000
VERSION     DASTJJ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407684"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFR02_05530"
     CDS             complement(<1..558)
                     /locus_tag="VFR02_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9869940.1"
                     /translation="MPLLTTDRGRFFCQEAGPGPGPALFLCHGLTSNHRAWERVEGPL
                     AGAGFHLFAFDMRGHGASDRPGSGYAPEDHARDLEACARALGLGRVQAVGHSTGGRNA
                     LVFAALFPALVAGLAVVDQTLSADAESWRKYQKRYGEYPVPFADEGALDAFLREKFPD
                     DARRFDYYKSQFWARPDGRWDFNFSI"
     gene            complement(566..886)
                     /locus_tag="VFR02_05535"
     CDS             complement(566..886)
                     /locus_tag="VFR02_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869941.1"
                     /translation="MATMKFPDKAAQAKLRKQVAQKLAQGRSKIQELEKKIRSGEAGR
                     EITAELKKAKAKLEKLKAQYKVQEKKAMDYVQKNPKKAVLAAAAVGLLAGAVLAALKS
                     SKKK"
     gene            complement(918..1250)
                     /locus_tag="VFR02_05540"
     CDS             complement(918..1250)
                     /locus_tag="VFR02_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869942.1"
                     /translation="MAKAKKTEENVGSGVLDWAAKAFQAWQKGLKPALALFAEKLEER
                     AERLERRVMGLLVAYLSFFAGVFFLALGSFFLLIDLAGLPRWAVFSGGGLLFLATSVV
                     YLKASRGR"
     gene            1380..>1723
                     /locus_tag="VFR02_05545"
     CDS             1380..>1723
                     /locus_tag="VFR02_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9869943.1"
                     /translation="MSTPAAHDPYAAFRHANYRFYASGNFISVLGQQMLRAAVGYEVY
                     QRTHSVFHLGLIGLAVWAPILLCMLPGGLAADRYSRKAIVLACQLAYAGASFGLALAA
                     AWAAPLWTLYALL"
BASE COUNT          287 a          610 c          500 g          326 t
ORIGIN      
        1 gatggaaaaa ttgaaatccc agcggccgtc gggccgggcc cagaactggc ttttgtaata
       61 gtcgaaccgg cgcgcgtcat ccgggaattt ttccctcagg aaggcgtcca gcgccccttc
      121 atcggcgaag ggcaccggat attcgccgta gcgtttttgg tatttgcgcc agctttcggc
      181 gtcggccgaa agggtctggt cgacgaccgc caggccggcc acaagcgccg gaaagagggc
      241 ggcgaagacc agggcgttgc gccccccggt ggaatggcct acggcctgga cccgcccgag
      301 ccccaaggcg cgggcgcagg cctccaggtc ccgggcatgg tcctcggggg cgtatcccga
      361 accgggccga tcggaggcgc cgtgtccccg catgtcgaag gcgaaaagat ggaaccccgc
      421 ccccgccaag ggcccttcca cccgctccca ggcgcggtgg ttggacgtga ggccgtggca
      481 caaaaaaagc gcggggcccg gcccgggccc cgcctcttgg cagaagaacc gtccccggtc
      541 cgtcgtcaat aaaggcaagg acgcctcatt ttttcttcga gcttttgagc gcggccagca
      601 cggcgcccgc caacaacccc acggccgccg cggccagcac ggcctttttg gggttcttct
      661 ggacatagtc catggccttc ttctcctgga ccttgtattg ggccttgagc ttttccagct
      721 tggctttggc ctttttgagc tcggccgtga tctcccggcc ggcctcgccg ctgcggatct
      781 tcttctccaa ctcctggatc ttggaacggc cctgggccag cttctgggcc acttgcttcc
      841 tcaacttcgc ctgcgccgcc ttgtccggaa atttcatcgt ggccacatcg ggcctccttg
      901 ttcgagttaa aagtgagtta tcggccgcgg gaagccttca ggtaaacgac ggatgtggcg
      961 aggaaaagca gcccgccgcc ggaaaagacc gcccagcgcg gcaatcccgc caggtcgatc
     1021 aacaggaaga acgatcccaa ggccaggaag aagacccccg caaagaacga aagataggcc
     1081 accaacaggc ccatcacacg gcgttccaag cgttccgccc gctcctccaa cttctcggcg
     1141 aaaagcgcca gggcgggctt caggcccttt tgccaggcct ggaaggcctt ggcggcccaa
     1201 tccagcaccc ccgagcctac gttctcctcc gtctttttcg ccttggccat cgcgcacctc
     1261 ccggttttcc ccatcctagc ccagggggcc ggaaaaagcc ccccctcccg tctttccctt
     1321 tgagcgcatt atcgtttaca atcccgcctc atgttatgac gtaggtcatg gagagaacga
     1381 tgtccacccc cgccgcccac gacccctacg cggccttccg ccacgccaat taccgctttt
     1441 acgcgtcggg caatttcatc agcgtgctgg gccagcagat gctccgcgcg gccgtcggct
     1501 acgaggtcta ccagcgcacc cattcggtct tccacctggg cctcattgga ctggccgttt
     1561 gggcccccat cctgttgtgc atgctccccg gcggcctggc ggccgaccgt tattcccgca
     1621 aggccatcgt gctcgcctgc cagttggctt acgccggcgc ctccttcgga ttggccctgg
     1681 cggcggcctg ggcggcgccc ctctggaccc tctacgcgct cct
//
LOCUS       DASTJJ010000452         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_794927, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000452 DASTJJ010000000
VERSION     DASTJJ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794927"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="bioF"
                     /locus_tag="VFR02_05550"
     CDS             <1..1093
                     /gene="bioF"
                     /locus_tag="VFR02_05550"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET9869944.1"
                     /translation="RLRLVDPLGGTAARVNGRDCVLFCTNNYLGLADHPRVLGWAHQA
                     LDRYGAGSQASRLVSGNFPIHELLEEKTALFKGTEACLAFPTGYMANLAAVSALVGEE
                     DAVLCDRLNHASLIDACRASRAKLLVYEHLNLDDLKGCLDRAQGYRRRLIVTDSLFSM
                     DGDLAPLPAIMELAGTYEALVLIDDAHGTGVLGVGGRGASHRFPLKTPPTAVVGTYSK
                     ALGSVGGFVCGDKVLADYLLNRARTFIYTTGLPPSVCGAALGALEVLEEEPDRVEKLH
                     RNAAKVRAGLQGLGFRVPPGESPILPVVVGENEKALAMADRLLEEGLLVVAIRPPTVP
                     KGTARLRLSVSSEHTEEQISRLLTAFQKL"
     gene            1126..1806
                     /gene="bioD"
                     /locus_tag="VFR02_05555"
     CDS             1126..1806
                     /gene="bioD"
                     /locus_tag="VFR02_05555"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET9869945.1"
                     /translation="MAGTDTGVGKSLISAGLARLWVNQGHSVGVLKPVASGSAKASPD
                     RSGGLISEDGKLLQKAAKLPNSAYASIVPVHYRQPLAPYVAAWKEGAVPLAKVEKAYR
                     EARAKYDRLIVEGIGGVLVPITKDFLAVDWQVKWELPTLVVARAGLGTINHTLLTLEA
                     LRRRKVKVLGVVVNGYKGKTIAERTNVRALRKLAGVRVYGPVGSNPKYRTNLDLLARD
                     LERSGLGR"
     gene            1958..>2360
                     /locus_tag="VFR02_05560"
     CDS             1958..>2360
                     /locus_tag="VFR02_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9869946.1"
                     /translation="MDHRKLAAKDKKYLWHPFTQQLEWESDPDITIVSRGKGTFLYDA
                     KGKKYLDGVSSLWCNVHGHRVPAIDAALKRQIGKIAHSTLLGASNEPAILLAEKLVKL
                     APRGLNRVFYSDSGSEANEIAAKMAFQFWKQT"
BASE COUNT          438 a          732 c          774 g          416 t
ORIGIN      
        1 ccgtctgcgc ctggtggatc ccctgggcgg cacggccgcc cgggtcaacg gccgggactg
       61 cgtgctgttc tgcaccaaca actacctggg gctcgccgac cacccccggg ttctgggctg
      121 ggcccaccag gccctggacc gctacggcgc cggttcccag gcttcccgcc tggtgtcggg
      181 caattttccc atccatgaac tgctcgagga aaagaccgcc cttttcaagg gcacggaggc
      241 ctgcctggcc ttccccacgg gctatatggc caacctggcg gccgtctcgg ccctggtggg
      301 ggaagaggac gcggtcctgt gcgaccggct caaccacgcc agcctcatcg acgcctgccg
      361 ggcttcccgg gccaaattgc tggtctatga gcacctcaac ctggacgacc tgaaggggtg
      421 cctggatcgg gcccagggtt accggcggcg cctcatcgtg accgactcgc tcttcagcat
      481 ggacggcgac ctggcgcccc tgcccgcgat catggaattg gccgggacct acgaagccct
      541 ggtgctcatc gacgacgccc atggcacggg ggtcttgggt gtgggggggc ggggggcgtc
      601 ccaccgattt cccctaaaaa cgccgccgac ggcggtggtg ggcacctaca gcaaggcgct
      661 tggcagcgtg ggcggtttcg tgtgcgggga caaggtcctg gccgattacc tgctcaaccg
      721 ggcccgcacc ttcatttata cgaccgggct tccgccctcg gtctgcggcg ccgccctggg
      781 ggcgctggag gtgctggaag aggaaccgga ccgggtggaa aagctccacc gcaacgccgc
      841 caaggtgcgg gcgggcctgc aggggctggg tttccgggtg cccccgggcg aaagccccat
      901 cctgcccgtg gtggtggggg agaacgagaa ggccctggcc atggcggacc ggcttttgga
      961 ggagggcctg ctggtcgtgg ccatccgccc gcccaccgtg cccaagggca cggcccgact
     1021 gcgtttgtcg gtctccagcg aacacaccga agagcagatc agccgactcc tgacggcctt
     1081 ccaaaaacta tgagcccggc gcgttccccg atccgcacac tttttgtggc cggcaccgac
     1141 acgggcgtgg gcaagtcgct catttcggcg ggactggccc ggctttgggt gaaccagggc
     1201 cattcggtgg gcgtgttgaa acccgtcgca tccggctccg cgaaggcttc gccggacagg
     1261 tccggtgggc ttatcagtga ggacggtaag ctgctccaaa aggccgccaa gctcccgaat
     1321 tcggcttatg cttccattgt ccccgtccat taccggcagc ccctggcccc ctatgtggcg
     1381 gcctggaagg aaggcgcggt gccactggcc aaggtcgaaa aggcctatcg ggaagcccgg
     1441 gcgaaatatg accgtttgat cgtggaaggc atcggcgggg tgttggtccc cattacgaaa
     1501 gacttcttgg cggtggattg gcaggtcaag tgggaactgc ccactctggt ggtggcccgg
     1561 gccgggttgg ggacgatcaa ccatacgctc ctgaccctgg aggccctgcg ccgccgcaag
     1621 gtgaaggtcc tgggcgtggt ggtgaacggt tacaagggta agaccatcgc cgagaggacc
     1681 aacgtgcggg ccctccggaa gctggcgggc gtgagggtct atgggccggt tgggtccaac
     1741 cccaagtacc ggaccaacct tgacctttta gcacgtgatt tggaaagatc gggccttggg
     1801 cgttaaagcg cgaaagaatt taaaggcaat tttcaccacc aaggcaccaa gacaccaaga
     1861 acggcggaac aaagaggatc ttgggatttc cccgaatgct tcgcctttct tggtgacctt
     1921 ggtgccttgt ggtcaaacga aggtgtttaa ggaaataatg gatcaccgaa aactcgccgc
     1981 gaaagacaaa aaataccttt ggcatccctt tacccagcag ttggaatggg aaagcgaccc
     2041 ggacatcacc atcgtgagcc gggggaaggg caccttcctc tatgacgcca aggggaaaaa
     2101 atacctggac ggcgtttcgt cgctctggtg caacgtgcac gggcaccggg tgcccgcgat
     2161 cgacgccgcg ctgaaaagac agatcgggaa gatcgcccat tcgaccctgt tgggcgcctc
     2221 caacgagccc gccatcctgc tggccgagaa actggtgaaa ctggcccccc ggggcctgaa
     2281 ccgggtcttt tattccgatt cgggttccga ggccaacgag atcgcggcca agatggcctt
     2341 ccaattctgg aaacaaacga
//
LOCUS       DASTJJ010000453         1491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_674090, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000453 DASTJJ010000000
VERSION     DASTJJ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674090"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFR02_05565"
     CDS             <1..554
                     /locus_tag="VFR02_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9869947.1"
                     /translation="KAKAGDARGAAAALGSTAGLGLVFLAVKAAGWAAEVHAGKLPST
                     NLFFGCYYALTGFEGLHVLIGVGVLLVLAWRAARGDFHARYYSPVLVTGLYWGLLAVV
                     WALILLFLYVSGGAMIRRILFFTALGWEPVWACPYCSTNLGQGCDGFGRGITLSIFLF
                     LGVVGLLAGGLVYQILRQEKDRG"
     gene            complement(559..1323)
                     /locus_tag="VFR02_05570"
     CDS             complement(559..1323)
                     /locus_tag="VFR02_05570"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HET9869948.1"
                     /translation="MNPTPWLIGFLALALPLGAQPVSLSTRAGGPAPVVLELFTSQGC
                     SSCPPAEALLGTWGLEQFTSGKLLPLAFHVDYWNDLGWVDPFSSADATGRQRDYARAL
                     GRDSLYTPQAVLGGQVDAVGSDAKALRERLAALSTRRAVTGLEISAARQGKSLKMTVE
                     TQGTGAAGGDWVLCLAVFENGLVTKVPRGENRGRDLEGAFVVRSFQKAYPAPASGVFS
                     VDWDPAWDPARTGVAAFLQDRRSLLIDSAAAVFPLR"
BASE COUNT          225 a          464 c          546 g          256 t
ORIGIN      
        1 ggaaggccaa ggcgggcgat gcccgggggg cggcggcggc cctggggtcc acggcgggtc
       61 tggggctcgt cttcctggcg gtgaaggccg ccggatgggc cgcggaagtc cacgcgggga
      121 aactgccctc caccaacctc tttttcggat gctattacgc cctgacgggg ttcgaggggc
      181 tgcacgtgct catcggggtg ggggtgctgc tggtcctggc ctggcgggcg gcccgggggg
      241 acttccacgc gcgctactac agtccggtgt tggtgacggg cctctattgg gggttgttgg
      301 cggtggtgtg ggccctcatc ctgttgttct tgtatgtttc agggggggcg atgatccgga
      361 ggatcctgtt cttcacggcg ttgggttggg agccggtttg ggcctgcccc tattgctcca
      421 ccaacttggg ccaaggctgc gacggcttcg ggcggggcat caccctttcc atcttccttt
      481 tcctgggcgt ggtgggcctg ctggccgggg gattggtcta ccagatcctc aggcaggaaa
      541 aggaccgggg ctgaaccctc agcgcagggg aaagaccgcc gcggccgagt cgatgagcag
      601 gctgcggcgg tcctgcagga aggccgccac gccggtccgg gccgggtccc aagccgggtc
      661 ccagtccacc gagaagaccc ccgaggcggg cgccggatag gccttttgga aggaccggac
      721 cacgaaggcc ccctccaggt cccggccgcg gttctccccg cggggcacct tcgtcaccaa
      781 gccgttctcg aacaccgcca ggcacaacac ccaatcgccc cccgccgcgc cggtcccctg
      841 ggtttccacg gtcattttca ggctttttcc ctggcgcgcg gcggatattt caaggcccgt
      901 gacggccctg cgggtcgaca gtgcggccag cctttcgcgc agcgccttgg cgtcgctgcc
      961 caccgcgtcc acctgtcccc ccaggacggc ctggggcgtg tagagcgaat cccggcccag
     1021 ggcccgcgcg tagtcccgct ggcgcccggt ggcgtcggcc gaggaaaagg ggtccaccca
     1081 tcccaggtcg ttccaatagt ccacatggaa ggcgaggggc agcagtttcc cggaggtgaa
     1141 ctgctcaagg ccccaggtgc cgagcagggc ttcagcgggg gggcaactgg aacagccttg
     1201 ggacgtgaaa agctccagca ccacgggggc cgggccgccg gcgcgggtgg aaagggagac
     1261 aggttgggcg cccagcggca gggccagggc caaaaaaccg atcagccagg gggttgggtt
     1321 catgcccttc tatacggggg aaaacgggaa aaagtgaccg ggcccggtca gacgtagatc
     1381 ttctgggccc cgctgaagag ggtcttgatg gcctcgctga tcttttggtt cacctgttcc
     1441 ttgcgcgggt cgtcgggcgc ggtgaaaggg gagtcgaaaa gagggaggac c
//
LOCUS       DASTJJ010000454         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_920407, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000454 DASTJJ010000000
VERSION     DASTJJ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920407"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..85
                     /locus_tag="VFR02_05575"
     tRNA            3..85
                     /locus_tag="VFR02_05575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Leu,seq:gag)
     gene            complement(162..>1005)
                     /locus_tag="VFR02_05580"
     CDS             complement(162..>1005)
                     /locus_tag="VFR02_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869949.1"
                     /translation="YNPTQKKYEKVYTEEGGQIQWIKVASDAPSKRNFLFLDRLDDSG
                     RQTLSAYACLGNGVQPVLQAQAPQVYAQFDGGAKGLSVLCSDKSMPQDAKDADHVFKW
                     NEAKSLFAETAAAPPAGWTGSSVAGTAVTTASASAAPAASPVPTASASAPPTAVVPTP
                     TVLAAAPAPAPPAPSGEWWGSPLDAPAASAKLDDEMVPAAVKSGQMAALGKNANAFFQ
                     KAKGSGLSTSAISQMRAGYYASVASTLSGMGRGKDAAYYLDLALKLDPSNPKALGLKA
                     KLKP"
BASE COUNT          168 a          316 c          340 g          181 t
ORIGIN      
        1 aggcccgagt ggtggaattg gcagacacgt acgcttgagg tgcgtatggg cgaccgtgcg
       61 ggttcgagtc ccgcctcggg caccacctga tcgaaaagac catggatcac gcgatccatg
      121 gtctttttgt tttatggccg acgttcccga acccgcgccg cttagggttt cagtttggct
      181 ttcaatccga gggccttggg gttggacggg tccagcttca gggccaggtc caggtaatag
      241 gccgcgtcct tcccccgccc catgccggaa agcgtcgagg ccaccgacgc gtaataaccg
      301 gcacgcatct gcgaaatggc ggaagtcgaa agtcccgatc ccttggcctt ttggaaaaag
      361 gcgttggcgt tcttgcccaa agccgccatc tgaccggact tgacggccgc cgggaccatc
      421 tcgtcatcca atttggccga cgccgcgggg gcatccaagg gggaacccca ccattcgccc
      481 gaaggggccg gcggtgccgg ggcaggcgcg gccgcgagca ccgtgggcgt gggaacgacc
      541 gccgtcgggg gcgcggaagc cgaggccgtc gggaccggcg aggccgcggg ggccgcggaa
      601 gccgaggccg tggtcacggc cgtcccggcc acggagctcc cggtccatcc ggcaggaggc
      661 gcggcggccg tctcggcgaa caacgacttg gcttcattcc acttgaacac gtgatcggcg
      721 tccttcgcgt cctgcggcat cgacttgtcc gagcaaagca ccgacagccc cttggccccg
      781 ccgtcgaatt gggcgtagac ctggggggcc tgggcctgca ggacgggctg gaccccgttg
      841 cccaaacagg cgtaggcact caacgtctgc cggcccgaat cgtccaaccg gtcgaggaat
      901 aagaagttcc gcttggatgg ggcgtccgag gcgaccttga tccattggat ctggccgcct
      961 tcttccgtat agaccttctc gtattttttc tgggtggggt tgtag
//
LOCUS       DASTJJ010000455         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1408669, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000455 DASTJJ010000000
VERSION     DASTJJ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408669"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFR02_05585"
     CDS             complement(<1..540)
                     /locus_tag="VFR02_05585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9869950.1"
                     /translation="MNTPFPSITVPGSPVNAKDHVEPGTGLYLVLAFLCALAGSVFLI
                     AVSYGVLLVAALFYPLFLGYLNKKAMALLNGSGVRVSESQFPEIHRALVAFKERLGIT
                     RDVAVYIVEANVLNAASIKYGRRDVVTITDDLIQGCLASGNPQALSFVLAHELGHIAL
                     RHTGVFRRWMTRFLKKLSRL"
     gene            complement(647..1267)
                     /locus_tag="VFR02_05590"
     CDS             complement(647..1267)
                     /locus_tag="VFR02_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9869951.1"
                     /translation="MPHLPIQAIFLDVGGVLLTNGWDRAARRAAAEKFGLDAADLEER
                     HHLTYDTYEVGKISLETYLDRVIFHVKRDFSREDFRSYMYAQTQALPGMIDYFTRLKQ
                     AHGLRVGVISNEGRELSEYRIQHFHLGELADFFVMSAFVHFRKPDTDIFKLALDIAQV
                     PARQCLYFDDRPLFVEVARRLGLHAVHHRGLASTQKAVEALGLTLP"
     gene            complement(1337..>1541)
                     /locus_tag="VFR02_05595"
     CDS             complement(1337..>1541)
                     /locus_tag="VFR02_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET9869952.1"
                     /translation="LSRRIGEHFQALVTGASDKGTWVRLLKPPVEGKLVQGARGLDVG
                     DALRVKLIEVDVERGWIDFVRTS"
BASE COUNT          290 a          472 c          534 g          245 t
ORIGIN      
        1 cagccggctg agcttcttca ggaagcgggt catccaacgg cggaaaaccc cggtgtgccg
       61 cagggcgatg tggcccaact cgtgggccag cacgaaggac agggcctggg ggttgcccga
      121 ggccaggcag ccctggatca ggtcgtcggt gatcgtgacc acgtcgcgcc gcccgtactt
      181 gatggaggcg gcgttcagga cgttggcctc cacgatgtag accgccacgt cccgggtgat
      241 gcccaggcgt tccttgaagg ccaccagggc ccggtggatc tcggggaact ggctctcgct
      301 gacgcgcacc ccggacccgt tgaggagcgc catggccttc ttgttcaggt agcccaggaa
      361 gaggggatag aagagggccg ccaccagcag gacgccgtag gagacggcga tgaggaagac
      421 cgaacccgcc agggcgcaga ggaaggccag gaccaggtaa aggcccgtgc cgggctcgac
      481 gtggtctttg gcgttgaccg ggctgccggg gacggtgatg gacggaaagg gtgtgttcat
      541 ccgttggact ccttgcggcg agatgggggc ctgaaaagaa aaaacaaccc gacaatcata
      601 gcctggacca aggacaaggc caaggatttt gggcgcggcc gcccctttaa ggcagggtga
      661 gccccaaggc ctccacggcc ttttgggtgg aggccaggcc ccggtggtgg acggcgtgga
      721 ggcccaaccg gcgggccacc tccacgaaca ggggccggtc gtcgaaataa aggcattggc
      781 gggcggggac ctgggcgatg tccagggcca gcttgaagat gtccgtgtcg ggcttgcgga
      841 aatggacgaa ggccgacatg acgaagaagt ccgccaactc ccccaggtgg aaatgctgga
      901 tgcggtactc ggaaagttcc cgcccttcgt tgctgatgac ccccaccctg aggccatggg
      961 cctgcttgag ccgggtgaag tagtcgatca tgccgggcag ggcctgggtc tgggcgtaca
     1021 tataggaacg gaaatcctcc cggctgaaat cccgcttcac atggaagatg acgcggtcca
     1081 ggtaggtctc caagctgatc ttgcccacct cgtaggtgtc gtaggtgagg tggtgccgct
     1141 cctccaggtc ggcggcgtcc aggccgaact tttccgccgc ggcccgccgg gccgcccggt
     1201 cccagccgtt ggtgagcagc acgcccccca cgtcgaggaa aatggcttgg atgggcaagt
     1261 ggggcatgga acgtcctttc gaatgtcgtt caacggatgg ccgcgccggc gcgccggacg
     1321 cggggaccac atggtttcag gaagtcctca cgaaatcaat ccagccccgt tccacgtcca
     1381 cttcgatgag cttgacgcgc agggcgtcgc ccacgtccag cccccgggcc ccctgcacca
     1441 gcttgccttc cacgggcggt ttcaacaacc gcacccaggt gcccttgtcc gaagcgccgg
     1501 tgaccagggc ctggaaatgc tccccgatcc ggcgggacag g
//
LOCUS       DASTJJ010000456         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_796113, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000456 DASTJJ010000000
VERSION     DASTJJ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796113"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFR02_05600"
     CDS             <1..327
                     /locus_tag="VFR02_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869953.1"
                     /translation="GQVVVHWTYDSSVVQFLTYHRVPLTPIDHPELPFDFQAQRDVVL
                     IFEVGEWSDYLPEMHRLYPGAEWSTLSTPWKQPFLWVVRIPKDELDKARGSRKPGVVL
                     PTGFPS"
     gene            378..1061
                     /locus_tag="VFR02_05605"
     CDS             378..1061
                     /locus_tag="VFR02_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9869954.1"
                     /translation="MDDLNEAVNYNRWIYEKVAPHLGKRVLEVGCGTGNITGFLARGR
                     QVLAVDIHQGYLDAAKKRWGRRPGLRFHRFDLERGLSAFKSFKPDTIISVNVLEHIDK
                     DRAFLKACYGLLPPGGRVLIFVPAFQWLYGSMDLSYGHHRRYTRRSLNERLEEAGFRV
                     SFCRYLNLLGVFGWFLNGRILRRKIIPKGQMLLYDRILKYVAPVEEYLPRPLGLSLLS
                     VAVKPGPRG"
     gene            1090..2595
                     /gene="lysS"
                     /locus_tag="VFR02_05610"
     CDS             1090..2595
                     /gene="lysS"
                     /locus_tag="VFR02_05610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9869955.1"
                     /translation="MSELNEFIKVRLDKLKALREKGIDPYPTRFDPTHHSIDILEKYK
                     DLQASEHREADKVAVAGRLVTIRGMGKSSFAHLLDAKGKVQIYVKLDGVGAEAYEVFK
                     MLDLGDIVGVKGFPFKTKTGEVSVHAESFVLLSKSLHPLPEKWHGLTDVEARYRHRYV
                     DLIVNEDVRKTFEARSKVVAAIRRYMDGQKFLEVETPMMHPIPGGAAAKPFVTHHNAL
                     DMDLYMRVAPELYLKRLLVGGLERVYEINRNFRNEGISIKHNPEFTMLEAYQAYGDHE
                     TVMTLTENLLAEAAQAAGRSLKIKVGEVELDFTPPFKRLPLLQALKEIGGVDYDKTDV
                     EELKRRVKGTPAQVKHIDSLSKAELAYLLFGELVEGKLIQPTFITEFPVEVSPLAKAK
                     ADDPALTERFELFVLGREIANGFSELNDPVDQRRRFEKQVALRKAGDEEGMFLDEDYI
                     LALSYGMPPAGGVGIGIDRLLMLLTDSPSIRDVILFPLLRPRGDEEKMVFE"
     gene            2735..3988
                     /locus_tag="VFR02_05615"
     CDS             2735..3988
                     /locus_tag="VFR02_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET9869956.1"
                     /translation="MNLPFEIFLGLRYLRSKRKRSAISVLTWLSVIGVAIGVMALNVV
                     LSVMNGYDAELKKDIVGINPHIVVSGYHNQPLADAGHFTRVISKMDHVVSAGPYVEGQ
                     ALARSRERAVGIMVWGVDPDHPQALADLDKYLYEAKATDLKPPSTEGSGRDARILLGS
                     VLAQRLQVGVGDDVLLFLPSMQMTPLGMTPQSMKFQVVGVFTTGMYDYDSDFAYVALP
                     MAQKMYALGDNVTGVAVKLDNVDLADRLGREIRTAFGGDYFVQDWLQVNRNLFTAIHM
                     EKIVMGIILSLIVLVAALNIVNPLTMMVIEKTKEIGILKAMGATDKSVNTIFLFEGML
                     IGVMGILVGLAAGFVVCEIIALVPIHIPGGGMVYYIDRLPVKVDPFVSYGVVPLVAVV
                     LCFLATLYPARQAAKLEPVDAIRYE"
     gene            3997..4677
                     /locus_tag="VFR02_05620"
     CDS             3997..4677
                     /locus_tag="VFR02_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9869957.1"
                     /translation="MEIIRLEDVTKSYHLDGEEIRALRRVNFSLDAGDITVILGPSGS
                     GKSTLLTLLGGLGKPSEGKVWCRGKSLYDLSNPELALWRNKVFGFVFQFHYLMPELTA
                     LENVMLAGRISRKTPEECLSRASKLLGHVGLSSRVGHKPGELSGGEQQRVAVCRALMN
                     EPEIILADEPTGNLDLKTAEGIHDVLWELCQKEGRTLIVVTHYEPLAKRADHILSLNG
                     GQVVKKKN"
BASE COUNT          869 a         1475 c         1496 g          868 t
ORIGIN      
        1 ggacaggtgg tcgtgcattg gacctatgat tccagcgtgg tccagttcct cacctaccac
       61 cgcgtcccct tgacgcccat cgaccatccc gagctgccct tcgatttcca ggcccaaagg
      121 gacgtcgtgt tgatcttcga ggtgggggag tggtccgact atctcccgga gatgcaccgt
      181 ctttatccgg gggccgaatg gtcgaccctg tccacgccct ggaaacaacc cttcctctgg
      241 gtggtgcgca tcccgaagga tgaattggac aaggcccggg ggagccggaa gccgggggtc
      301 gtgcttccca cgggtttccc ttcgtgaagg cccgttccgc acgcggggcg aggaagtggg
      361 acggccagcg ggagaccttg gacgacctga atgaggcggt caactacaac cgctggatct
      421 acgagaaggt cgcgccccac ctgggcaagc gggtcttgga agtgggctgc ggtacgggca
      481 acatcacggg tttcctggcc cgggggcgcc aggtgctggc cgtcgacatc caccagggtt
      541 acttggacgc ggccaagaag cgttggggac gccggccggg cctgcggttc caccggttcg
      601 acctggaaag gggcttgtcg gccttcaagt ccttcaagcc cgacaccatc atcagcgtga
      661 acgtgctgga acacatcgac aaggaccgcg ctttcctgaa ggcctgttat ggcctcctgc
      721 cgcccggggg acgggtgctg atcttcgtgc cggcgttcca atggctctat ggatcgatgg
      781 acctgagtta cggccaccac cgccgctaca cccgccgcag cctgaacgag cgcctggaag
      841 aggcgggttt ccgggtgtcc ttctgccgtt atttgaacct gttgggggtc tttgggtggt
      901 tcctgaacgg ccgtattttg cggcgcaaga tcattcccaa gggtcagatg cttctttatg
      961 atcgtatcct caagtacgtc gcgccggtgg aggaatatct gccgcggccc ctcgggcttt
     1021 ccctcttatc cgtggccgtc aagccgggcc cccggggctg agacggccag tccagaaaag
     1081 gttggtgcca tgtccgaatt gaacgaattc atcaaggtgc ggttggacaa gctcaaggcc
     1141 ctgcgcgaaa aggggatcga tccctatccc acgcggttcg accccacgca tcattccatc
     1201 gacatcctgg aaaagtacaa ggacctgcag gcctccgagc accgggaggc cgacaaggtc
     1261 gccgtcgccg ggcgcctggt caccatccgg ggcatgggga agagctcctt cgcccacctg
     1321 ttggacgcca agggcaaggt ccagatctac gtcaagctgg acggcgtggg cgcggaggcc
     1381 tacgaggtct tcaagatgct ggacctgggc gacatcgtgg gcgtgaaggg tttccccttc
     1441 aagaccaaga ccggggaggt gtcggtccac gccgagtcct ttgtccttct ctccaaatcc
     1501 ctgcaccccc tgcccgagaa gtggcacggc ctcaccgacg tggaggcccg ttaccgccac
     1561 cgttacgtgg acctgatcgt caacgaggac gtgcgcaaga ccttcgaggc ccgctcgaag
     1621 gtggtggcgg ccatccgccg ttacatggac ggccagaagt tcctggaggt ggagacgccc
     1681 atgatgcacc ccatccccgg gggcgcggcg gccaagccct tcgtgacgca ccacaacgcg
     1741 ctggacatgg acctctacat gcgggtggcg cccgagcttt acttgaagcg cctcttggtg
     1801 ggcgggctcg agcgggtcta cgagatcaac cgcaacttcc gcaatgaggg aatcagcatc
     1861 aagcacaacc ccgaattcac catgctggag gcctaccagg cctacggcga ccatgagacg
     1921 gtcatgacct tgaccgagaa cctcttggcc gaggccgcgc aggccgcggg ccggtcgctc
     1981 aagataaagg tgggggaggt ggagctggac ttcacccctc ccttcaaacg cctgcccctg
     2041 ctgcaggccc tgaaggagat cggcggggtg gattacgaca agaccgacgt ggaagaactc
     2101 aagcgccggg tgaagggcac gcccgcccag gtcaagcaca tcgactcctt gagcaaggcc
     2161 gaactggcct acctgctctt cggggaactg gtggaaggca agctgatcca acccaccttc
     2221 atcaccgaat tcccggtgga ggtctcgccg ctggccaagg ccaaggccga cgaccccgcg
     2281 ctcacggagc gttttgagct ctttgtgctg gggcgggaga tcgccaacgg tttttccgaa
     2341 ttgaacgatc ccgtcgacca gcgacgccgc ttcgagaaac aggtggcgct gcgcaaggcg
     2401 ggggacgaag agggcatgtt cctggacgag gactatatcc tggccctgtc ctacggcatg
     2461 ccgcccgccg ggggcgttgg catcggcatc gaccggctct tgatgctatt gaccgacagc
     2521 ccctccatcc gcgacgtcat cctgttcccg ttgcttcgcc cccgcggcga tgaggaaaag
     2581 atggtttttg agtgatccgt gaaagcccat tttcaccacc aaggcaccaa gacactaagg
     2641 actgcgatga cgaagacccg ggtggtcgat ctgcttttct tggtgtcttg gtgccttggt
     2701 ggtgatgatc tgcgttggat gaattgaggt tttcttgaac cttccttttg aaatattcct
     2761 gggccttcgc tacctgcgct ccaagcgcaa gcgctcggcg atctccgtcc tcacctggct
     2821 ctcggtcatc ggggtggcca ttggggtgat ggcgctcaac gtggtgctgt ccgtcatgaa
     2881 cggttacgac gccgagctca aaaaggacat cgtgggcatc aacccccaca tcgtggtgtc
     2941 gggctaccac aaccaacccc tggccgacgc gggccacttc acccgggtca tctccaagat
     3001 ggaccacgtg gtctccgccg ggccctacgt ggaaggccag gccctggccc ggtcccgcga
     3061 gcgggcggtg ggcatcatgg tctggggcgt ggaccccgac catccccagg ccctggcgga
     3121 cctcgacaag tacctttatg aggccaaggc caccgacctc aagccccctt ccaccgaggg
     3181 cagcggccgg gacgcccgca tcctgttggg cagcgtgctg gcccagcgcc tgcaggtggg
     3241 cgtgggggac gacgtgctgc tctttttgcc cagcatgcag atgacgcccc tggggatgac
     3301 gccccagagc atgaagttcc aggtggtggg cgtcttcacc acgggcatgt acgactatga
     3361 ctcggacttc gcctacgtcg ccctgcccat ggcccagaag atgtacgccc tgggcgacaa
     3421 cgtgaccggg gtggcggtga agctggacaa cgtggacctg gccgaccggc tgggccggga
     3481 gatccgcacg gccttcgggg gcgattattt cgtgcaggac tggctgcagg tgaaccgcaa
     3541 cctcttcacg gccatccaca tggaaaagat cgtgatgggc atcatcctgt ccctgatcgt
     3601 gctggtggcg gccctcaaca tcgtgaaccc gctcaccatg atggtcatcg agaagaccaa
     3661 ggagatcggc atcctgaagg ccatgggcgc caccgacaag agcgtgaaca ccatcttcct
     3721 cttcgagggc atgctgatcg gcgtcatggg catcctggtg gggctggcgg cggggttcgt
     3781 ggtctgtgag atcatcgcgc tcgtgcccat ccacatcccg ggagggggca tggtctatta
     3841 catcgaccgc ctgccggtga aggtggaccc cttcgtcagc tacggggtgg tgcccctggt
     3901 ggcggtggtg ctgtgcttcc tggccaccct ttatcccgcg cgacaggcgg ccaagctgga
     3961 gcccgtcgac gccatccgct acgagtaagg ggagccatgg agatcatccg cctcgaggac
     4021 gtgaccaaga gctaccacct ggacggggag gagatccgcg ccctgcgccg ggtcaatttc
     4081 tcgctggacg cgggggacat cacggtcatc ctgggcccct cgggctcggg caagagcacg
     4141 cttttgacgc tcctgggcgg cctgggcaag ccctcggagg gcaaggtctg gtgccggggc
     4201 aagtcgctct acgacctgtc caaccccgaa ctggcgcttt ggcgcaacaa ggtcttcggt
     4261 ttcgtctttc agttccatta cctgatgccc gagctgaccg ccctggagaa cgtcatgctg
     4321 gccgggcgca tttcccgcaa aaccccggaa gaatgcctct cccgcgcgtc caaactcctg
     4381 gggcacgtgg ggctgtcctc ccgggtgggc cataagcccg gggagctttc cggcggggaa
     4441 cagcagcgcg tggcggtctg ccgggcgctc atgaacgaac ccgagatcat cctggccgac
     4501 gagcccacgg gcaatctgga cctgaagacc gccgagggca tccacgacgt gctctgggag
     4561 ttgtgccaga aggaaggccg caccctcatc gtggtgacgc actacgaacc cctggccaaa
     4621 cgcgccgacc acatcctgag cctcaacggc gggcaagtgg tgaagaaaaa gaattaaagc
     4681 atttaccacg aagttcacaa agttcacg
//
LOCUS       DASTJJ010000457         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_63826, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000457 DASTJJ010000000
VERSION     DASTJJ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63826"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFR02_05625"
     CDS             complement(<1..359)
                     /locus_tag="VFR02_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9869958.1"
                     /translation="MRRRDRRNNVLMLYGAITGFGIFLGLVFPLLDKVQTPSPLWFYV
                     ICVSCGICAAALILLIVQKIVLKPLEEFLNAMEKVTKGDLSVSIHGHHTGDFEALAQN
                     FNMMIEYLRSSVVQLKK"
     gene            complement(356..1351)
                     /gene="ruvB"
                     /locus_tag="VFR02_05630"
     CDS             complement(356..1351)
                     /gene="ruvB"
                     /locus_tag="VFR02_05630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9869959.1"
                     /translation="MPEKDREEDENPLRPQTLRDYVGQSALKDSLSIALEAAQKRQEA
                     LEHLLLAGPPGLGKTTLASIIAREMGVNLRPTSGPVIERPGDLAALLTNLQPHDLLFI
                     DEIHRLPRAVEEILYPAMEDFQLDILIGKGPTARSIRLQLPPFTLVGATTRAGTVSAP
                     LRDRFGLHFRLEFYTQEELVEIVKRSAQVLKVALAGEGAVEIARRSRGTPRIANRLLK
                     RVRDFAEVRRSGKVDAGVADAALRLLEVDALGLDRMDRRLLSCVIEKFGGGPVGLETL
                     AIAVGEESETLEDVYEPFLVQLGFLQRTPNGRKATAAAYAHLGLTPPSGPQGSLL"
BASE COUNT          237 a          448 c          482 g          240 t
ORIGIN      
        1 cttttcttga gctggacgac cgaggatcgc aggtactcga tcatcatgtt gaaattctgg
       61 gccagggcct caaaatcgcc cgtgtggtgt ccgtggatgg aaacgctcag gtcgcccttg
      121 gtgacctttt ccatggcgtt caggaactcc tccaggggtt tgaggacgat cttctggacg
      181 atgagcagga tgagcgcggc cgcgcagatg ccgcaggaaa cgcaaatgac gtagaaccag
      241 agcgggctgg gggtctggac cttgtccagc agggggaaca ccaggcccaa aaagataccg
      301 aagcccgtga tggccccata gagcatcagg acgttgttgc gccggtcccg ccgcctcaca
      361 agaggctccc ttgggggccc gagggcgggg tcaaacccag gtgggcgtag gccgccgccg
      421 tggccttgcg gccgttgggc gtgcgctgca ggaaccccaa ttggaccagg aagggctcgt
      481 aaacgtcctc cagggtctcg gattcctcgc ccaccgcgat ggccagggtc tccaaaccca
      541 ccggcccccc cccgaatttt tcgatgacgc aggaaagcag gcggcggtcc attcggtcca
      601 gccccagggc gtccacttcc agcagccgca gggccgcgtc cgccaccccg gcgtccacct
      661 tgccgctgcg ccggacctcg gcgaaatccc gcacccgctt gagcagccgg ttggcgatgc
      721 ggggcgtgcc ccgggaacgc ctggcgatct ccaccgcgcc ttccccggcc agggccacct
      781 tcaggacctg ggccgatcgc ttgacgatct ccaccaattc ctcctgggtg tagaactcca
      841 gacggaaatg caggccgaaa cggtcgcgca agggcgcgct cacggtcccg gcgcgggtgg
      901 tggcccccac cagggtgaag ggcggcagtt gcaggcggat ggagcgggcc gtggggccct
      961 tgccgatgag gatgtccaat tggaaatcct ccatggccgg atagaggatc tcctcgacgg
     1021 cccgggggag ccggtggatc tcgtcgatga acaacaggtc gtggggttgc aggttggtca
     1081 ggagggcggc caggtccccc ggccgctcga tgaccgggcc cgaagtcggc ctcaggttca
     1141 cgcccatctc ccgcgcgatg atcgaggcca aggtggtctt ccccagcccc ggaggcccgg
     1201 ccaggagcag gtgttccagg gcctcctggc gtttttgggc cgcctccagg gcgatggaaa
     1261 gggaatcctt cagggcgctt tggcccacgt aatcccgcag ggtctggggc cggagggggt
     1321 tttcgtcctc ttcccggtcc ttctcgggca tcaggacgcg cgtttcaggg ggcagctcgg
     1381 gggaaggcgt ctcgggaagt ttcagtt
//
LOCUS       DASTJJ010000458         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1654437, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000458 DASTJJ010000000
VERSION     DASTJJ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654437"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFR02_05635"
     CDS             complement(<1..1185)
                     /locus_tag="VFR02_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869960.1"
                     /translation="MKQNAAQKLNRLFLALGLGLAAPAWADHGPLSSLARDGWVNGSV
                     EDLVAAGLAPDPGKPAAQMTNLEVAELTAQAARRVLAQADPSLLPPPLPGDAAEMGGP
                     GLPAPLSAEASAPSQSLEALAQEFRGELTAMGVDLDQLEDRLADQADRNRVFSTLQQD
                     YLKRTGTEVGGSSRGFMNNYRGIGNNALYPPMGYNAAMFLEMDLKSVPVPDLLFDARF
                     RFWRSIGMYYQDPVQPSYQLRWISLTSYNDFGNFTGGDFYKSYTPLTLWNYDVPVYTF
                     VEPTSFHRTRLDTEELVYMDQAPNWHFRGFQADTGKDWDKDPVLSGFKYQLMGGLINL
                     PGTTGGSAVFGSYYLGSQGSLSLFDHDLTVTGTGLMLYDDKGSANVPYLVNVPSTYPR
                     EYQ"
     gene            complement(1220..>1567)
                     /locus_tag="VFR02_05640"
     CDS             complement(1220..>1567)
                     /locus_tag="VFR02_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869961.1"
                     /translation="QIPGGGTQADNPNLPLSGAIPNLFEQRVFSGALMVQVVKDIDLV
                     GDMGVETWKSDYTYPRVDYQTNEYGLGVAYDIPWCGGKLECRYKHIDFHDVDVPNNDY
                     SGDQFFSKVKFMF"
BASE COUNT          254 a          483 c          547 g          283 t
ORIGIN      
        1 ctggtactcg cgggggtagg tggaaggcac gttcaccagg tagggcacat tggcgctgcc
       61 cttgtcgtcg tagagcatca agcccgtgcc ggtgacggtc aggtcgtggt cgaagaggga
      121 caggcttccc tggctgccca ggtagtagct gccgaagacc gccgaaccgc ccgtggtgcc
      181 cggcaggttg atgaggcccc ccatgagctg gtacttgaag ccgctcagga ccgggtcctt
      241 gtcccagtcc ttgcccgtgt cggcctggaa gccccggaaa tgccaattgg gcgcctggtc
      301 catgtagacc agttcctcgg tgtccaggcg cgtgcggtgg aaggacgtgg gttccacgaa
      361 ggtatagacc ggcacgtcgt agttccagag ggtgaggggc gtgtagctct tatagaagtc
      421 gcccccggtg aagttgccga agtcgttgta gctggtcagg gagatccagc gcaattgata
      481 ggaaggctgg accgggtcct ggtaatacat gccgatggag cgccagaaac ggaagcgcgc
      541 gtcgaacaag aggtcgggca ccggcacgct cttgaggtcc atctccagga acatggccgc
      601 gttgtagccc atggggggat agagggcgtt gttgccgatg ccccggtagt tgttcatgaa
      661 gccgcgcgac gagcccccca cctcggtgcc ggtgcgcttc aggtaatcct gctgcagggt
      721 ggagaagacg cggttgcggt cggcttggtc cgccaagcgg tcctccagtt ggtccaggtc
      781 cacgcccatg gcggtgagtt cgccccggaa ctcctgggcc agcgcttcca ggctctggct
      841 gggcgccgag gcctcggcgg aaagcggcgc cggcaggccc ggtccgccca tttcggcggc
      901 gtcccccggg agcggcggcg gcaacaagct ggggtcggcc tgggccagga cgcggcgcgc
      961 ggcttgggcg gtcagctcgg cgacctccag gttggtcatc tgggccgcgg gcttgcccgg
     1021 gtccggggca aggcctgccg ccaccaggtc ctccacgctg ccgttcaccc aaccgtcgcg
     1081 cgccagggac gacagggggc cgtggtccgc ccaagcgggc gcggccaagc ccagccccag
     1141 ggccaggaag aggcggttca gtttttgagc ggcgttctgt ttcatccaaa cctcggcttc
     1201 aagttggccc ggcgggcccc tagaacatga acttcacttt gctgaagaac tggtcgcccg
     1261 aatagtcgtt gttgggcacg tcgacgtcgt ggaaatcgat gtgcttgtag cggcattcca
     1321 gcttgccccc gcaccagggg atgtcgtagg ccacgcccag gccgtattca ttggtctgat
     1381 agtccacccg gggataggta tagtccgact tccaggtctc cacgcccatg tcccccacca
     1441 ggtcgatgtc cttcaccacc tgcaccatca aggccccgct gaagacccgc tgctcgaaga
     1501 ggttggggat ggcgccggag agcggcaggt tcgggttgtc ggcctgcgtg ccgccgcccg
     1561 ggatctg
//
LOCUS       DASTJJ010000459         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_187327, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000459 DASTJJ010000000
VERSION     DASTJJ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187327"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFR02_05645"
     CDS             <1..435
                     /locus_tag="VFR02_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HET9869962.1"
                     /translation="TVISGDPAAVTRAGEGCKARGAKRVIGLKVSGAFHSPLMAPAVP
                     ALREALGAAGFQDPRFPVIANASATAVATAGTAVTLLAEQLTAPVRWVECQREAARLA
                     PGAPYVELGPGTVLSGLLKKIVPGAVSVPLSSADDVEKFLAA"
     gene            476..>1161
                     /locus_tag="VFR02_05650"
     CDS             476..>1161
                     /locus_tag="VFR02_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HET9869963.1"
                     /translation="MTSPVDLSGKTAFVTGSTRGIGRAVAVILHEAGAKVAILGRDAG
                     RAQAVAAEIGEGARGFACDVGDPGSIQAAVEGVTRELGPVDILVNNAGITRDNLLLRM
                     GEDEWDQVLDANLKGAFRTVKAVLKGMMKRGGGRIINITSIVGLTGNKGQANYAASKA
                     GLIGFTKSVAKEYASRGILANCVAPGFIETDMTAALPDDARASLLQDIALGRLGRPED
                     VAGAVLFLAS"
BASE COUNT          177 a          379 c          421 g          184 t
ORIGIN      
        1 acggtcatct ccggtgatcc tgctgcggtg acccgggcag gcgaagggtg caaggcccgg
       61 ggggccaaac gggtcatcgg cctcaaggtg agcggcgcct tccactcccc cttgatggcg
      121 cccgccgtgc ccgcgctacg cgaggcgctg ggtgccgcag ggttccagga tccccgcttt
      181 ccggtcatcg ccaatgccag tgccacggcg gtggccactg ccgggaccgc ggtgaccctg
      241 ttggccgagc agctgacggc cccggtacgc tgggtcgagt gccagcgtga ggccgcccgc
      301 ctcgcgccag gggcgccgta tgtcgagctt ggcccgggca ccgtgctctc cggattgctc
      361 aagaagatcg tcccgggtgc cgtcagcgtt ccgctcagca gtgctgacga cgtggaaaag
      421 ttcctcgccg cctgagcggc caccgatcga ccgtccactg tccaggccga gcctcatgac
      481 cagccctgtc gatctttccg gcaagaccgc cttcgtgacc ggcagtaccc gcggcatcgg
      541 gcgtgcggtg gcggtcatcc tccacgaggc cggcgccaag gtggccatcc tgggccgtga
      601 cgcgggccgg gcccaggccg tggccgccga gatcggtgag ggggcgcggg gctttgcgtg
      661 tgacgtgggc gatcccgggt cgatccaggc cgcggtggag ggggtgacca gggagctggg
      721 tccggtggac atcctggtca acaatgccgg gatcacgcgg gacaacctcc tgctccggat
      781 gggcgaggac gagtgggatc aggtcctgga cgccaacctg aagggcgcgt tccgtaccgt
      841 gaaggcggtc ctcaagggga tgatgaagcg gggcgggggg cggatcatca acatcaccag
      901 catcgtcggg ctcaccggca acaaggggca agccaactac gccgccagca aggccgggct
      961 catcggcttc accaagtcgg tggccaagga gtacgccagc cgcggaatcc tggccaattg
     1021 cgtggccccg gggttcatcg agaccgatat gaccgccgcc ttgcccgatg acgcgagggc
     1081 ctcattatta caggacattg ctttgggccg cctggggcgg cctgaggatg tcgccggggc
     1141 ggtgctgttt ctggcctccg a
//
LOCUS       DASTJJ010000460         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_796823, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000460 DASTJJ010000000
VERSION     DASTJJ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796823"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1024
                     /locus_tag="VFR02_05655"
     CDS             134..1024
                     /locus_tag="VFR02_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9869964.1"
                     /translation="MTKREKRNLALGLAFTGPWIVGFSAFTLYPALASFAYSLCDYSV
                     LTKPVFIGLGNYRDLFTDQVYWKSVFNTFFYALLYLPLGTLFSLGLALLLNTGVRGQA
                     LYRTLFFLPSVIPMISMAILWLWMFNPHYGLVNHALALVGLPGPQWLQDPHWIKPAFA
                     LMGVWTVGNAVVIYLAALQDVPRSLYEAAELDGAQWHHKIRHVTLPMISPAIFFNVVM
                     GLIATLQVFAVPYVMTFPVPGQPARSALFYTMYLYESAFRYLRMGYACAMAVVMFLII
                     LGLTWLFMRLSKRHVHYRGS"
     gene            1027..>1558
                     /locus_tag="VFR02_05660"
     CDS             1027..>1558
                     /locus_tag="VFR02_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9869965.1"
                     /translation="MAPASRRRREAWGRACAHLLLLAGAVFFAAPLLWMLSTSLKSVG
                     QAMAQPPQWIPHPVQWGNYLDAVRYIDFFRYTANSLTVCLLSVAGTLLSSSLAAYGFS
                     RIEWKGRDAFFLLTLSTMMIPFPTLMVPLYGVFRELHWIGTLKPLWVPACFGSAFSIF
                     LLRQFFLGIPQEITEAA"
BASE COUNT          214 a          577 c          438 g          329 t
ORIGIN      
        1 tctggtacct gcaaaaaacg cccgcccagg ccctgggcga gatgcagggc cgcatgcagc
       61 ggctttggac ccgggacctg cgccgcttga agcggcgggg cctgggcccg gacgacgcca
      121 cggagggaca gccatgacca aacgggaaaa acgcaacctg gccttgggat tggccttcac
      181 gggcccttgg atcgtgggct tttcggcctt caccctttat ccggccctgg cgtccttcgc
      241 ctacagcctg tgcgattact cggtgctgac caagcccgtc ttcatcgggc tgggcaacta
      301 tagggacctc ttcaccgacc aggtctattg gaaatcggtc ttcaatactt tcttctacgc
      361 cctgctgtac ctgcccctgg gcaccctctt ttcgctgggc ctggccctgt tgctcaacac
      421 gggcgtccgg ggccaagcgc tgtatcggac cctcttcttc ctgccttccg tcatccccat
      481 gatctccatg gccatcctct ggctctggat gttcaacccc cattacgggc tggtgaacca
      541 cgccctggcc ctggtgggcc tgcccggccc ccagtggctg caggaccccc attggatcaa
      601 gccggccttc gccctgatgg gggtctggac ggtgggcaac gccgtggtca tctacctggc
      661 cgccctgcag gacgtccccc ggtcgctcta tgaggccgcc gagctggacg gcgcccaatg
      721 gcaccacaag atccggcacg tgacgctgcc catgatctcc ccggccatct tcttcaacgt
      781 ggtcatgggg ctcatcgcca ccctgcaggt cttcgccgtg ccctacgtca tgaccttccc
      841 cgtgcccggc caaccggccc ggtcggcgct cttctacacc atgtacctct acgagagcgc
      901 cttccgctac ctgcgcatgg gttacgcctg cgccatggcg gtggtgatgt tcctcatcat
      961 cctggggctg acctggctct tcatgcgcct gtccaagcgg cacgtgcatt accgggggtc
     1021 ctgaccatgg cccccgccag ccggcgccga cgggaagcct gggggcgcgc ctgcgcccac
     1081 ctgttgctct tggccggggc ggtctttttc gccgcccccc tgctctggat gctctccacc
     1141 tcgctcaagt ccgtgggtca ggccatggcc cagccgcccc aatggatccc ccatcccgtc
     1201 caatggggca actacctgga cgccgtgcgc tacatcgact ttttccgcta cacggccaat
     1261 tcgctcaccg tctgcctgct gtcggtggcc ggaaccctct tgtcttcctc gctggcggcc
     1321 tacggttttt cccgcatcga atggaagggg cgggacgcct tcttcctgct caccctgtcc
     1381 accatgatga tccccttccc caccctcatg gtgccgctct acggcgtctt ccgggaactg
     1441 cactggatcg ggaccctgaa gcccctctgg gtgcccgcct gcttcgggtc ggccttcagc
     1501 atcttcctgc tgcgccagtt cttcctgggc atcccccagg agatcaccga ggccgcgc
//
LOCUS       DASTJJ010000461         1479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1043534, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000461 DASTJJ010000000
VERSION     DASTJJ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043534"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..847
                     /gene="rpsB"
                     /locus_tag="VFR02_05665"
                     /pseudo
     CDS             179..847
                     /gene="rpsB"
                     /locus_tag="VFR02_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            1280..>1479
                     /gene="tsf"
                     /locus_tag="VFR02_05670"
     CDS             1280..>1479
                     /gene="tsf"
                     /locus_tag="VFR02_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107181.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET9869966.1"
                     /translation="MEVTAKMVSDLREKTGAGMMDCKKALTETSGDFDKAVAYLRERG
                     AAQVAKRSGRAASQGVIGTYVHG"
BASE COUNT          318 a          469 c          478 g          214 t
ORIGIN      
        1 ccggttttat gctataacac cgcacccgcg gggcttgtcc cgcgcgggga ggcagggacc
       61 cagtcccgcc tatttcgcac gcttgtcctg gccctccagt gtcccgcccg tcgggatggc
      121 gagggtcccc gcctcgggga acggagcaag cggtggtcca acttaaagga gctttcccat
      181 gatgtccgtc tcgatgaagc agttgctgga agccggggtc catttcgggc accaaacccg
      241 gcggtggaac cccaagatgg ccgcctacat tttcggcgaa cgcaacggca tccacatcat
      301 cgacctgcag aagaccgtca agaaactcaa ggaagcctac cagtacgtca aggacatgtc
      361 ggcggcgggc gagaccatcg tcttcgtcgg caccaagaaa caggcccagg acgccatcaa
      421 ggaagaggcc gaacgctgcg gcatgttcta cgtcaactac cgttggctgg gcggcatgct
      481 gaccaacttc tccaccatca aaaagtccat cagctacctg aaggaactgg aaagcatctc
      541 ggacctggag atggccaagc gctccaagaa ggaacaggcc atcctgacca aggacaagga
      601 gaagctgcag cggtccctgg gcggcatcaa gaacctgggc aagctgcccg gcgccatctt
      661 cgtcatcgac accaagaaag agcacatcgc ggtgctggaa gcccgcaagc tgggcatcaa
      721 ggtcatcgcc gtggtggaca ccaactgcga tcccgagctc atcgacgtgg tggtgccggg
      781 caacgacgac gccatccggg cggtcaagct catgacctcc gtcatggcca gcgccatcct
      841 ggaaggccgg tccaaggagc tggagggccg cgaggtctcc gccgagggcg ctgcgggcct
      901 cagcgaggtg ggcgaggagc tgttggccgc cgcccggcag gaagcgagca tccccgcgga
      961 gggcgagacc gtcgccgagg aagccgagga gaagccggag gccgagaaga aggaagcggc
     1021 cgaactgctg gtctccgacg tcagccactt ggccggcgac atcaccaccg tcgccgcctt
     1081 ggccgacgaa ctggccaacc ggaaagtgga acgcatccgc accaagcgca cccgcgacga
     1141 ataatcaagg acctccttca ccgcgaagac gcaaaggcgc gaagagaggc ttcccggtcc
     1201 ggtccagcat ccctttgcgt ctctgcgcct ttgcggtgga ccgggtttcg accttcaaaa
     1261 tttcaggagg ctgttcgaca tggaagtgac cgccaagatg gtgagcgacc tgcgcgagaa
     1321 gaccggcgcg ggcatgatgg actgcaagaa ggccctgacc gagacgtcgg gcgacttcga
     1381 caaggccgtg gcctacctgc gcgagcgcgg ggcggcccag gtggccaagc gttcgggccg
     1441 ggccgcctcc cagggcgtga tcggcaccta cgtgcacgg
//
LOCUS       DASTJJ010000462         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1776470, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000462 DASTJJ010000000
VERSION     DASTJJ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776470"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFR02_05675"
     CDS             <1..519
                     /locus_tag="VFR02_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869967.1"
                     /translation="GLEADLPNLSTKTPLGEVPLILGPTGLYARWEPFHFGGWNSSTA
                     GALSFKAGGAYAEANSVFSRIASSWTLAVLGGYRLGPWFVQGNYGYNGGFDGRAGEWQ
                     AHTDLGLGLGSDWYVQTELDLDLREGPTGGASWTLLPQVAFQPGEWLFEFGESFNGSP
                     AGYTEILAARTF"
     gene            complement(527..>1249)
                     /locus_tag="VFR02_05680"
     CDS             complement(527..>1249)
                     /locus_tag="VFR02_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869968.1"
                     /translation="AAKEAYGLISNLPEGGYVKLGKFLVPYGLGLSDDNSLVRLPLEA
                     AFSFDGPTPDGVEVGLAPSPLFLSAALVNGASNPGSGSVAGYDEKAFSARGGLNFPEW
                     TLGGSVYGENLDLDNGRLRYGTYGWVRMGPAVFLGELDAGWDALGTGLPLPPAGWTDY
                     LATHASVEFDMGGSVYLRLTDEWLDDSRRLNPHDGLRNMVSLRCYPVGNLKLQVDLAR
                     MDPVLAVNDPDYSLVADAFVFY"
BASE COUNT          195 a          414 c          440 g          200 t
ORIGIN      
        1 gggctggagg cggacctccc caatttgtcc accaagacgc ccctggggga agtgccgctc
       61 atcctggggc ccaccggcct ctacgcgcgt tgggaacctt ttcatttcgg cggatggaac
      121 agctcgaccg cgggcgccct gtccttcaag gcgggcggcg cctacgcgga ggccaacagc
      181 gtcttcagcc ggatcgccag cagttggacc ctggcggtcc tggggggcta ccggctgggc
      241 ccctggttcg tccaggggaa ttacggctac aacggcgggt tcgacgggcg cgcgggcgag
      301 tggcaggccc acacggacct gggcctgggt ttggggtcgg attggtatgt ccagaccgaa
      361 ctggacctgg acctccggga aggcccgacg ggcggggcat cctggacgct ccttccccag
      421 gtcgccttcc agccgggcga gtggctgttc gagttcgggg agtcgttcaa cgggagcccg
      481 gcgggttaca cggagatcct ggcggcccgg accttttgac gttccctcaa taaaagacga
      541 aggcatccgc gaccaggctg tagtcggggt cgttgacggc caggacgggg tccatgcggg
      601 ctaggtcgac ctgcagcttg aggttgccca ccgggtagca ccgcaggctg accatgttgc
      661 gcaggccgtc gtgcgggttg aggcgccggg aatcgtccag ccattcgtcg gtcagccgca
      721 ggtagacgga gccgcccatg tcgaattcca cgctggcgtg ggtggccagg tagtcggtcc
      781 atccggccgg gggaagcggc aggcccgtgc ccagggcgtc ccaccccgcg tccagttcgc
      841 ccaggaagac cgcgggcccc atccggaccc acccataggt cccgtagcgc agcctcccat
      901 tgtccaggtc caggttctcc ccgtagaccg acccgcccag ggtccattcg gggaaattca
      961 ggccgccgcg ggccgagaag gccttctcgt cgtatccggc caccgacccg gacccggggt
     1021 tggacgcccc gttgaccagg gccgcgctga ggaacagggg ggacggcgcc aggcccacct
     1081 ccacgccgtc gggcgtgggg ccgtcgaaac tgaaggccgc ttccagggga agacgcacca
     1141 agctgttgtc gtcgctcaat cccaggccgt aggggaccag gaatttgccg agcttcacat
     1201 agcccccctc gggcaggttg gagatcagcc cgtaggcttc cttggcggc
//
LOCUS       DASTJJ010000463         1416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1776591, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000463 DASTJJ010000000
VERSION     DASTJJ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776591"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1416
                     /locus_tag="VFR02_05685"
     CDS             <1..>1416
                     /locus_tag="VFR02_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9869969.1"
                     /translation="IARALTNQPRLILADEPTGNLDSKSSEEIIGFLGRMNESGITVV
                     LVTHEPDIAAHARRVITMKDGKVLSDERSHRSKVPAPRAVKAPELKKPAVTHASVNLR
                     QAGEYFHQALRALLANKVRSALSVLGILIGVAAVIAMLAVGTGAQKSLEQQLSSLGTN
                     LLTLMPGSMRVGGVQEGVGGTSRITLADAQALAAVPMVKRVAPESNGHVQVTYADKNW
                     NTSLTGTTPNYAEMKASEPVYGRFLTDDDVRRSNRVALLGLTVIKNLFGDNASPVGEW
                     IKIDRVSFQVIGVLPPKGSNGFQDRDDVIVVPVTTAMRRLFGKQYVNSVDIEADSAED
                     TGPVQDAVVNYMVKLKHIPDLPGSPASDAFHVRNMADIQATLSASTQIMTILLSSIAA
                     ISLLVGGIGIMNIMLVSVTERTREIGLRKAIGARRVDILSQFLIASVAVSLLGGFIGI
                     LLGWGVTLVISLAAGWATVVTP"
BASE COUNT          242 a          509 c          447 g          218 t
ORIGIN      
        1 atcgcccggg ccctcaccaa ccagccccgg ctcatcctgg ccgacgaacc cacgggcaac
       61 ctggattcca aaagctccga agagatcatc ggcttcctgg gccggatgaa cgagagcggc
      121 atcacggtgg tattggtgac ccatgagccc gacatcgcgg cccatgcccg gcgcgtcatc
      181 accatgaagg acggcaaggt gctttccgac gagaggagcc accggtccaa ggtccccgcg
      241 ccccgggccg tcaaggcgcc cgagttgaaa aagcccgcgg tcacccacgc ctccgtgaac
      301 ctgcgccagg cgggcgagta tttccaccag gccctgcgcg cccttttggc caacaaggtc
      361 cggtccgccc tgtccgtgct gggcatcctc atcggggtgg cggccgtcat cgccatgctg
      421 gcggtgggca cgggcgccca gaagtcgctg gagcaacagc tctcctcgct gggcaccaac
      481 ctgctcaccc tcatgcccgg gtccatgcgg gtgggcggcg tccaggaagg cgtgggcggc
      541 accagccgca tcaccctggc cgacgcccag gccctggccg ccgtgcccat ggtcaagcgg
      601 gtggcccccg agtccaacgg ccacgtgcag gtgacctacg ccgacaagaa ctggaacacc
      661 tcgctgacgg gcaccacgcc caattacgcc gaaatgaagg cttccgagcc cgtctatggg
      721 cgtttcctca ccgacgacga cgtgaggcgc agcaaccggg tggcgctgct ggggctcacc
      781 gtcatcaaga acctcttcgg ggacaacgcc agcccggtgg gcgaatggat caagatcgac
      841 cgggtcagtt tccaggtcat cggcgtgctg ccccccaagg gctccaacgg cttccaggac
      901 cgggacgacg tcatcgtggt gcccgtcacc accgccatgc gccggctctt cggcaagcag
      961 tacgtcaact cggtggacat cgaggcggac agcgccgagg acacgggccc cgtgcaggac
     1021 gccgtggtga actacatggt caagctcaag cacatccccg acctgcccgg cagccccgcc
     1081 tcggacgcct tccacgtgcg caacatggcc gacatccagg ccaccctcag cgcctccacc
     1141 cagatcatga ccatcctctt gtcctccatc gcggccattt cgctgctggt gggcggcatc
     1201 ggcatcatga acatcatgct ggtgtcggtc accgagcgca cccgggagat cgggctccgg
     1261 aaggccatcg gggcccgccg ggtggacatc ctgtcccagt tcctcatcgc gtcggtggcg
     1321 gtcagcctgc tgggcgggtt catcggcatc ctgctgggct ggggggtgac cctggtcatt
     1381 tccctggcgg cgggctgggc caccgtggtg acgccc
//
LOCUS       DASTJJ010000464         1310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_64571, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000464 DASTJJ010000000
VERSION     DASTJJ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64571"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..>1310
                     /gene="dnaB"
                     /locus_tag="VFR02_05690"
     CDS             204..>1310
                     /gene="dnaB"
                     /locus_tag="VFR02_05690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9869970.1"
                     /translation="MPPQDLEAEQSVLGAMMLSQDAIRTVIPICRAEDFYREAHRKIY
                     QAVVALDDRKEPVDVITLTNELKRTNDFEMVGGLSALSSLIDRVPTAANAEYYAQIVR
                     EKSAARRLITAATQVVADAYSESQPIKELLDQAEHRIFEATELRDTKDFSSIKDLIGR
                     SYEYVNDLAQHSVHITGVPTGFYDLDELTAGFQPSEMIVVGARPSMGKTSLALNIAQH
                     ACMGTNSLKKKYAVGFFSVEMTAKQLALRLLCSVARIDLKKARSGHLSQAEVGMLVNV
                     TGQFYDTKLFIDDTSSLSVLELKAKARRLKRQHGIDLLMIDYLQLIQAGIRTDNREQE
                     IAYISRQLKALAKDLDIPVIVLSQLSRPQKGQEEK"
BASE COUNT          265 a          464 c          376 g          205 t
ORIGIN      
        1 tgaccgtcga ggtccaggcc ctgtagataa ccggtggatg gtttttgtgg ataagtccgg
       61 cgcccgcccg ggcccgccaa gaaaaattcc gcgacccgtc cacccgcggt taaaataagc
      121 ccgtcgacca tccgccctcc ggagccccca tggaagtcct tcgtaaaacc tcaccctcct
      181 cacccgtctc atccgaggtg cgcgtgccgc cccaggacct ggaggccgag cagtccgtgc
      241 tgggcgccat gatgctgtcg caggacgcca tccggaccgt catccccatc tgccgggccg
      301 aggatttcta ccgggaggcc caccgcaaga tctaccaggc cgtcgtggcc ctggacgacc
      361 gcaaggaacc ggtggacgtc atcacgctca ccaacgagct caagcgcacc aacgacttcg
      421 agatggtggg ggggctgtcc gccctgtcct cgctgatcga ccgggtgccc accgccgcca
      481 acgccgaata ttacgcccag atcgtgcggg aaaagtcggc ggcgcgccgc ctcatcacgg
      541 cggccaccca ggtggtggcc gacgcctact cggaaagcca gcccatcaag gaactgctgg
      601 accaggccga gcaccgcatc ttcgaggcca ccgaactccg cgacaccaag gacttttcca
      661 gcatcaagga cctgatcggt cgaagctacg agtacgtcaa cgacctggcc caacacagcg
      721 tgcacatcac gggcgtgccc acgggcttct acgacctgga cgagctcacc gccgggttcc
      781 agccctccga gatgatcgtg gtgggcgccc ggccctccat gggcaagacc agcctggccc
      841 tcaacatcgc ccaacatgcc tgcatgggca ccaacagcct gaagaagaag tacgcggtgg
      901 gtttcttctc ggtggaaatg accgccaaac agctggccct gcgcctcttg tgctcggtgg
      961 cccgcatcga cctcaagaag gcccgctcgg gccacctgag ccaggccgag gtgggcatgc
     1021 tggtgaacgt cacgggccag ttctacgaca ccaagctctt catcgacgac acctcgtccc
     1081 tgtccgtgct ggagctcaag gccaaggcca ggcgcctcaa gcgccagcac ggcatcgacc
     1141 ttttgatgat cgactacctg cagctcatcc aggcgggcat ccgcacggac aaccgggaac
     1201 aggagatcgc ctatatttcc cgccagctga aggccctggc caaggacctg gacatcccgg
     1261 tcatcgtgct ttcccagctt tcccgccccc agaaggggca ggaagaaaag
//
LOCUS       DASTJJ010000465         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_310430, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000465 DASTJJ010000000
VERSION     DASTJJ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310430"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..865)
                     /locus_tag="VFR02_05695"
     CDS             complement(47..865)
                     /locus_tag="VFR02_05695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9869971.1"
                     /translation="MRSELDFFRAYVVSNPIAHSLWRCFECARFAREKLASPVLDVGC
                     GDGFFTRTVFRRRLDWGVDPNDREVHRARRGGNYKRVLRAGVAALPLEDASVRTVISN
                     CVLEHVPELDRGLSEIARVLKPGGRLLLTVPSEYYNRDSFYQRLFQGLGFNQAAQWYN
                     RSLNRVFKHYHVHGWRAWERKLHAKGLVLEKAEYIMPRKAFRLYDQLLMLAVPGKILR
                     ALTGRWVLWPRPWLPGILAPLVRGFLTEQDDKGIVYFIVARKMAKKRGGRTPSA"
     gene            complement(888..>1201)
                     /locus_tag="VFR02_05700"
     CDS             complement(888..>1201)
                     /locus_tag="VFR02_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET9869972.1"
                     /translation="ISKFANSFLKWLLRIEQQDCSGGYKCYRVSLLRSIGLDRIFSKG
                     YAFQVEILYRALRSGARVEEIPIIFVNRHEGESKLTLAELAAFARTVFQLRWMSWRGR
                     L"
BASE COUNT          257 a          401 c          324 g          219 t
ORIGIN      
        1 ttttccaggc ttttcaatta tcccatcggg accatgatct ccgtggtcaa gcgctagggg
       61 tccttccacc ccgctttttg gccattttgc gcgccacgat gaaatagacg atccccttat
      121 cgtcctgttc cgtcaaaaaa ccacgcacca agggcgctaa gatccctggc agccatggcc
      181 tgggccacag gacccaccgg ccggtcaggg cccgcaagat cttgcccggc acggccaaca
      241 tcagaagctg gtcgtaaagc cgaaaagcct tccggggcat gatgtattcc gctttttcca
      301 gcaccaatcc cttcgcgtgc aatttccttt cccatgcccg ccacccgtga acgtggtaat
      361 gtttgaagac ccggttgagg gaccggttgt accactgcgc cgcctggttg aaccccaggc
      421 cctggaacag gcgttgataa aaggaatccc ggttgtaata ttcgctgggg acggtcaaaa
      481 gcagccgtcc cccgggcttg agcacccggg cgatctcgga caatccccgg tccaattccg
      541 ggacatgttc caaaacgcaa ttgctgatga ccgtccggac gctggcatcc tccaaaggca
      601 aggcggccac accggcccgc aggacacgct tgtagttccc tccccgccgg gcccgatgga
      661 cttcccggtc gttcggatcc accccccaat ccaggcggcg ccggaagacc gtccgggtga
      721 agaagccgtc cccgcacccc acgtccaaga ccggggacgc caatttttcc cgggcgaaac
      781 gggcgcactc gaaacaacgc caaagcgagt gggcgatggg attggagacc acgtaggccc
      841 tgaaaaaatc cagttcggat ctcatggcgc gtccgccggg cccagggtca aagccggccc
      901 cgccagctca tccaccgcag ttggaagacc gtccgggcaa aggcggccaa ttcggccaac
      961 gtcagcttgc tttccccctc gtgccggttc acgaaaatga tcgggatctc ttccacccgg
     1021 gccccggacc gaagcgcccg gtagaggatc tccacctgga aagcgtaccc cttggaaaat
     1081 atccgatcca acccgatgga ccgcaacaag gacacccggt agcatttgta gccccccgaa
     1141 cagtcctgct gttcgatccg gagcaaccac ttgaggaaac tgttggcgaa tttgctgatg
     1201 a
//
LOCUS       DASTJJ010000466         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1044145, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000466 DASTJJ010000000
VERSION     DASTJJ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044145"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFR02_05705"
     CDS             <1..511
                     /locus_tag="VFR02_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869973.1"
                     /translation="HYDKALAFLQVARDKDPNDADPYVKMGMIHASRGQHEEALKMYR
                     KVMELDPQDASIHNVMGYIYDKQGEMERALEEYNKARELNPEDALILDNLGNLYYKLG
                     RFEKAQEEFQELIRLNPQHVDALSKLGNIYLKMGDKPKAVAHWEKALELDASNEILKN
                     NLKIVRGGV"
     gene            511..1143
                     /locus_tag="VFR02_05710"
     CDS             511..1143
                     /locus_tag="VFR02_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9869974.1"
                     /translation="MAMRVQEKSLGELLRQAGKITSRQIQVALAEQKKTREPIGKVLV
                     RLGFVQEQDILQVMEGMLVLTFRVENEFFGVETYRVREVLKHSPLLPAALGGPYWAGA
                     YSLRGNLLPVLSFRRVLGKEELDGGWFVVLERKGYLFLLWVDQVLEVRRFKVDQIEPV
                     PAYLYGKNTEIYYCLGKVKEDLYSVLNPDVLVDEQNLKTALTEARHALPT"
     gene            1151..>2615
                     /locus_tag="VFR02_05715"
     CDS             1151..>2615
                     /locus_tag="VFR02_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9869975.1"
                     /translation="MIREMAHGVDQGDASTHNNLGVFYYSKGMYAESIKEFKTALEVE
                     PENIQALENLKAVNRHTGLYDRSIEAFKKSVQMFPMDAEAHYNLANAYRYTGQYEPAI
                     AEYTKTLELNEAHPFARNYLGVAQKCLGHLDEAVAEFKKAIEKNPYFADLHNNLGEIY
                     YKKAMVNEAMIEFKKAISLNPDFAIAHYNLSFVFGDKNMLEDAEREFKKAVDLNPNFG
                     QGAQALLAIERPKSLEEKVAEQAAARPRDEAPQIDTYLSLAAAYRGKGLLDEAIREYK
                     RAIEAAPVEDRLYRNLGEVYLQKGLLEDALMTHQKAVQLGPDHIENYMQLGVALRELG
                     RSEEAARQFEKAIELVPHSSMAHFELGVTFFKMGESERATREVRQALEIDPDNAEAHY
                     YLGVYYYKVNLFDEAIREINQAIELNVSIASSAEALTYLGLALADKGRFEEAVIEYKK
                     ALEFDPRNPIIHNSLGVAYKNAGQAKEAVQHYEQAIAY"
BASE COUNT          575 a          787 c          837 g          416 t
ORIGIN      
        1 ccattatgac aaggccttgg ccttcctgca ggtggcccgg gacaaggacc cgaacgacgc
       61 cgacccctac gtgaagatgg gcatgatcca cgccagccgg ggccagcacg aggaagccct
      121 gaagatgtac cgcaaggtca tggagttgga cccccaggac gccagcatcc acaacgtgat
      181 gggctatatc tacgacaagc agggcgagat ggagcgggcg ctggaggaat acaacaaggc
      241 ccgcgaattg aaccccgagg acgccttgat cctggacaac ctgggcaacc tctattacaa
      301 gctgggacgc ttcgagaagg cgcaggagga gttccaggaa ctgatccggt tgaacccgca
      361 gcacgtggac gcgctttcca aactggggaa catctacctg aagatgggcg acaagcccaa
      421 ggcggtggcc cattgggaaa aggcgttgga attggacgcc tccaatgaga tcctcaagaa
      481 caacctgaag atcgtccggg ggggcgtcta atggccatgc gggtccagga aaaatcgctc
      541 ggagagctcc tgcggcaggc gggcaagatc acgtcccgcc agatccaggt ggccctggcc
      601 gagcagaaaa agacccggga acccatcggc aaggtgttgg tgcgcctggg tttcgtgcag
      661 gagcaggaca tcctgcaggt gatggaaggc atgttggtgt tgaccttccg cgtcgagaat
      721 gagttcttcg gcgtggagac ctaccgggtc cgggaggtgc tgaagcattc gcccctcctg
      781 ccggcggccc tgggcgggcc ctattgggcg ggcgcctatt cgctgcgcgg gaaccttttg
      841 cccgtcctgt ccttccggcg ggtgctgggc aaggaggagc tggacggggg ctggttcgtg
      901 gtgctggaac gcaagggtta cctgttcctc ctctgggtgg accaggtgtt ggaggtgcgc
      961 cgtttcaagg tggaccagat cgaacccgtg ccggcctacc tttacgggaa aaacacggag
     1021 atctattatt gcctgggcaa ggtgaaggag gacctctatt cggtcctcaa tcccgacgtc
     1081 ctggtggacg aacagaacct gaagaccgcg ctgaccgagg cccgacatgc cctcccaacc
     1141 tgagacccgg atgatccgtg agatggccca cggcgtggac caaggcgacg cctcgaccca
     1201 caacaacctg ggcgtcttct attattccaa gggcatgtac gccgagtcca tcaaggagtt
     1261 caagaccgcg ctggaagtgg agccggagaa catccaagcc ctggagaacc tcaaggcggt
     1321 caaccgccat acgggcctgt acgaccgttc cattgaggcc ttcaagaaat cggtccagat
     1381 gttccccatg gacgccgagg cgcattacaa cctggccaac gcctaccgct acacgggcca
     1441 gtacgagccg gccatcgccg agtacaccaa gacgctggaa ctgaacgagg cccatccctt
     1501 cgcccgcaac tacctggggg tcgcccagaa gtgcttgggc cacctggacg aggcggtggc
     1561 cgagttcaag aaagccatcg aaaagaaccc ctacttcgcc gacctgcaca acaacctggg
     1621 ggagatctac tacaagaagg ccatggtcaa cgaggccatg atcgagttca agaaggccat
     1681 ctcgctcaat cccgatttcg ccatcgccca ctacaacctg agcttcgtgt tcggcgacaa
     1741 gaacatgttg gaggacgccg agcgggagtt caagaaggcc gtggacctga accccaattt
     1801 cgggcagggg gcccaggccc tgttggccat cgagcgcccc aaaagcctgg aggagaaagt
     1861 ggccgaacag gcggccgccc gtccgcggga cgaggccccc cagatcgaca cctacctgag
     1921 cctggccgcg gcctaccggg gcaagggcct gctggacgag gccatccggg agtacaagcg
     1981 cgccatcgag gcggccccgg tcgaggaccg gctctaccgg aacctgggcg aggtgtacct
     2041 gcagaagggc ctgttggaag acgccttgat gacccaccag aaggccgtgc agttggggcc
     2101 ggaccatatc gagaactaca tgcagttggg cgtggcgctg agggaattgg gccggtccga
     2161 ggaggccgcc cggcagttcg agaaggccat cgagctggtg ccccattcca gcatggcgca
     2221 tttcgagttg ggcgtgacct tcttcaagat gggggagagc gagagggcca cccgggaggt
     2281 ccggcaggcc ctcgagatcg acccggacaa cgccgaggcc cactattacc tgggcgtcta
     2341 ttattacaag gtcaacctgt tcgatgaggc catccgggag atcaaccagg ccatcgagct
     2401 caacgtgtcc atcgcttcca gcgccgaggc cctgacctac ctgggcttgg cgctggccga
     2461 caagggccgc ttcgaggagg cggtcatcga gtacaagaag gccctggagt tcgacccgcg
     2521 caaccccatc atccacaaca gcctgggggt ggcctacaag aacgcgggcc aggccaagga
     2581 ggccgtccag cattacgagc aggccatcgc ctaca
//
LOCUS       DASTJJ010000467         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1655794, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000467 DASTJJ010000000
VERSION     DASTJJ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655794"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2123
                     /locus_tag="VFR02_05720"
     CDS             <1..>2123
                     /locus_tag="VFR02_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HET9869976.1"
                     /translation="PLLSVPNHWKTWAYAALLLLTVFTRFDHLGAKPHHHDESMHAFY
                     SFQLFQDGDYEYNPMMHGPFQFHGNAFMYYLFGVSDATSRYLAATFGVLTVVLAMMLG
                     PFIGRTGAFLATLLIVASPSFMYFDRFCREDAYMAGATFAMVVFLFRYLRSRKPLDFW
                     LAAVGFTVAFCTKESIFLTLAVVGTYLFIRLLPWFDVLVAGGLTTIGLLTQLIIPAGD
                     PMAAPAFLLFAGSAFLYTLFKLYVVWTEHRRDHSGGDLWDAIDGLGWDNLKFEWLGLG
                     AWWLVTIAVLKVSALQSIGNAWPVSLFLMLSYLGLIFRFGWLWLRNVSPALTGALTLS
                     MAIFTLLFTTFFTIGADQPDFASRIHYLLHGIYNGAFGGLEYWHGQQGVHRGDEPWYY
                     YLLQLPANEMVSFFFSAVALAYYGLFNRKPMPLFLGYWYVGSLALFSWAGEKMPWLIL
                     HPLLPSLLLTAYLLGDLWDSKPLRHTARAARAAAFLLFGVLLSYSLHSAVLLSFYHEA
                     NPVEPLVYVQSGEDCKRVVDWIDKISYGETGGKDIGLTIEDKCSWPFAWFLRDYHKRG
                     HPPVITSADNPIILTATESDAQVYPILSKAGYVNRKYKLRVWWVPSWFKKGYPSTDMN
                     MDLFRDWFFRNFIPLGSPRTDMVDWTDLKNWILYRQVWSDMGSYNMRMWVRPDLAQKY
                     GFTQTDRSDVPTDYPMAEPTPTTHH"
BASE COUNT          333 a          741 c          578 g          471 t
ORIGIN      
        1 gccgctcctt tccgtgccca accattggaa gacctgggcc tatgcggcct tgctgttgct
       61 gacggtcttc acccgcttcg accacttggg cgccaaaccg caccaccacg acgaaagcat
      121 gcatgccttc tattcgttcc agctgttcca ggacggcgac tacgagtaca accccatgat
      181 gcacggtccc ttccagttcc acggcaacgc cttcatgtac tacctgttcg gggtttcgga
      241 cgccacctct cggtacctgg ccgccacgtt cggcgtgctg accgtggtct tggccatgat
      301 gctgggaccc ttcatcggcc gcacgggcgc cttcctggcc accctgctga tcgtcgcctc
      361 cccgagtttc atgtatttcg accgtttctg ccgggaagac gcctacatgg ccggggccac
      421 cttcgccatg gtggttttcc tcttccggta cctgcggtcg cgcaaaccct tggacttttg
      481 gttggccgcc gtgggcttca ccgtggcctt ctgcaccaaa gaatcgatct tcctgaccct
      541 cgccgtggtg gggacctacc tcttcatccg gctgcttccc tggttcgacg tactggtggc
      601 gggcggcctc accaccatcg gccttctcac ccaactgatc atcccggcgg gggatcccat
      661 ggccgcgccc gctttcctgc tgttcgccgg gtccgccttc ctttacaccc ttttcaagct
      721 ttatgtggtc tggacggaac accgccggga ccattccggc ggcgacctct gggacgccat
      781 tgacgggctc ggctgggaca acctgaagtt cgagtggctg gggctggggg cctggtggct
      841 cgtgaccatc gccgtgttga aggtctccgc ccttcaatcc atcgggaacg cctggccggt
      901 cagcctcttc ctgatgctca gctacctggg cctcattttc cgcttcggat ggctctggct
      961 gcgcaacgtc tcccccgccc tgaccggcgc gctgaccctg tcgatggcca tcttcaccct
     1021 gctcttcacc accttcttca ccatcggcgc cgaccagccg gatttcgcct cccgcatcca
     1081 ttacctgctc cacggcatct acaacggggc ctttggcggc ctggagtact ggcacggcca
     1141 acaaggcgtc caccgcgggg atgagccctg gtattactac ctgctccagt tgcccgccaa
     1201 cgagatggtc tccttctttt tctcggcggt ggccctggcc tactacggtc tcttcaaccg
     1261 gaaacccatg ccgctcttcc tgggttattg gtacgtcggc tccttggcgc ttttctcctg
     1321 ggccggggaa aagatgcctt ggctgatcct gcatcccctc ctgccttccc tgctgctgac
     1381 cgcttacctg ttgggggacc tttgggattc caagcccctg cgccacaccg cgcgggctgc
     1441 ccgggccgcc gccttcctgc tgttcggggt gctcttgagc tactcgctcc actcggcggt
     1501 cctgttgagc ttctaccacg aggccaaccc ggtggaaccc ctggtctatg tgcagtccgg
     1561 cgaggactgc aaacgggtgg tcgattggat cgacaagatc tcctacgggg agaccggggg
     1621 caaggacatc ggcctgacca tcgaggacaa atgttcctgg cccttcgcct ggttcctccg
     1681 ggattaccat aagcgcggcc atcccccggt catcacctcg gcggacaacc ccatcatcct
     1741 gaccgccacc gaatccgacg cccaggtcta tcccatcctg agcaaggccg gttacgtgaa
     1801 ccgcaagtat aaactgaggg tctggtgggt gccgtcctgg ttcaaaaagg gctatccctc
     1861 cacggacatg aacatggacc tgttccggga ttggttcttc cgcaatttca tcccgctggg
     1921 ctcgccccgg accgacatgg tggactggac cgacctgaag aactggatcc tctaccggca
     1981 ggtctggtcc gacatgggca gctataacat gcggatgtgg gtaaggccgg acctggccca
     2041 aaaatatggg ttcacccaaa cggaccgatc ggatgttccc accgactatc cgatggccga
     2101 accgaccccc accacccacc acc
//
LOCUS       DASTJJ010000468         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1410382, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000468 DASTJJ010000000
VERSION     DASTJJ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410382"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFR02_05725"
     CDS             <1..396
                     /locus_tag="VFR02_05725"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869977.1"
                     /translation="PVDAANEDDLIKKADKSLYQAKSKGRNQVVAFEPMIKIPISYKP
                     SREVSKVALVGSFNNWDKEADYMHRQSDGSYQFVISLNPGVYHYKFVLNGVEWIPDPA
                     NPIREEDSLGGENSVLRVSGEASYYPTGA"
     gene            487..>2976
                     /gene="ileS"
                     /locus_tag="VFR02_05730"
     CDS             487..>2976
                     /gene="ileS"
                     /locus_tag="VFR02_05730"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9869978.1"
                     /translation="MAEYKSTLNITQTAFPMKANLPQTEPQRLQLWQDWGFHGRRPGR
                     EGAPQYLLHDGPPFANGDIHMGHALNKVLKDIIVKYRSLRGFEAPYVPGWDCHGMPIE
                     HKVMEKLGSKAKTMGKVEIRKECRAYAQKFLERQKEEFKRLGILGDFDHPYRTMDPSY
                     ELSLVKALTRLLGEGYVSRRLRPIHWCPVCRTALAEAEVEYADHESLAVTVAFPAKEL
                     PAGVLPQTPKDKLSFLIWTTTPWTLPANLGISVHPDFDYVSVKVGEAYYLVAKALLGP
                     VAKACGWPEGGYAVVEEVRGAKLDRAMARHPFLDQDSLVMVGAHVTADAGTGLVHTAP
                     GHGREDYEIGLKYQLPVYSPVNEAGRYDQRVKEYEGQTVWEANPKIVEKLKGLGRLLS
                     VETIKHSYPHCWRSKNPIIFRATAQWFIELDHRDLRQKCLSEIEKVQWIPAWGKDRIR
                     NMVEGRTDWCISRQRSWGVPITAVYCGACGQVVGDPKTLAATVALVEKEGIDGWFGHG
                     PEAVLPAGSICACGSKDFIKEEDILDVWFESGSSHFAVLDEREELRESPGRPSRFVLY
                     LEGSDQHRGWFQHALWTGVALKGRAPFSGVLTHGWVLDGEGKAMHKSAGNVIDPLEMV
                     KKYGADVLRLWVSSENYTEDVGISEEMLNRMSEAYRRIRNTFRFILGNLFDFDPVNNS
                     VADDQLLDIDAWAKMAANGNLHLINGYYEEYQFTKIFHQIDNFFAVELSSKYFDILKD
                     RLYTSEKNSKNRRSAQTVLYKILKDYVVAIAPILSFTAEEIWQSMPESYRSEKSVFLS
                     SFPDFEAEIAELCVSRRVNAVLYLDEWARIFY"
BASE COUNT          619 a          882 c          904 g          571 t
ORIGIN      
        1 ccggtggacg ccgccaacga ggacgacctc atcaagaagg ccgacaagag cctgtaccag
       61 gccaagtcca agggccgcaa ccaggtggtg gccttcgagc ccatgatcaa gatccccatt
      121 tcctacaagc cttcccggga agtctccaag gtggcgctgg tgggcagctt caacaattgg
      181 gacaaggaag cggactacat gcaccgccag tccgacggtt cctaccagtt cgtcatctcg
      241 ctcaacccgg gggtctacca ctacaagttc gtgctcaacg gcgtggagtg gatcccggac
      301 ccggccaacc cgatccgcga ggaggactcc ctgggcggcg agaacagcgt gttgagggtc
      361 tcgggcgagg cgtcctacta tcccacgggg gcctgacggg cttttatggc ttgcagccct
      421 ttttaagatg ctttagactt tcgtctcttg ccttctggaa ccagaaagac gcgcaccggg
      481 aaccccatgg ccgagtacaa atccaccctc aacatcaccc agaccgcctt ccccatgaag
      541 gccaacctgc cccagaccga gccccaacgg ctccaactgt ggcaggactg gggtttccac
      601 ggacgccgcc cgggccggga aggcgccccc cagtacctgc tgcacgacgg cccgcccttc
      661 gccaacggcg acatccacat ggggcacgcc ctcaacaagg tcctcaagga catcatcgtc
      721 aagtaccggt cgttgcgggg tttcgaggcc ccctacgtgc cgggctggga ctgccatggc
      781 atgcccatcg agcataaggt catggaaaag ctgggctcca aggccaagac catgggcaag
      841 gtggagatcc gcaaggaatg ccgggcctac gcccagaagt tcctggaacg ccaaaaggaa
      901 gaattcaaac gcctgggcat cctgggcgat ttcgaccatc cctaccggac catggaccct
      961 tcttatgaac tgtccctggt caaggccttg accaggctgc tgggcgaggg ctacgtgagc
     1021 cggcggcttc ggcccatcca ttggtgcccg gtctgccgca ccgccctggc cgaggccgag
     1081 gtggaatacg ccgaccatga gtccctggcc gtgacggtgg ccttcccggc gaaggaactg
     1141 ccggccgggg tgctgcccca gacccccaag gacaagcttt ccttcctcat ctggaccacc
     1201 accccctgga cgctcccggc caacctgggc atttcggtgc acccggattt cgactatgtc
     1261 tccgtaaagg tgggggaggc ctattacctg gtggccaagg ccctgttggg gccggtggcc
     1321 aaggcctgcg gctggcccga gggcggctac gcggtggtgg aggaggtgcg gggggcgaag
     1381 ttggaccggg ccatggcccg gcatcccttc ttggaccagg attcgctggt gatggtgggc
     1441 gcccatgtca ccgccgacgc cggcaccggc ctggtgcata ccgcccccgg ccacggccgg
     1501 gaggactacg agatcggcct caagtaccaa ttgccggtct attcacccgt caatgaggcg
     1561 ggccgttacg accagcgggt gaaggaatac gagggtcaga ccgtctggga agcgaacccc
     1621 aagatcgtgg aaaagctcaa ggggctgggt cggctcctgt ccgtggagac gatcaagcac
     1681 agctaccccc attgctggcg ctccaagaac cccatcatct tccgggccac cgcccagtgg
     1741 ttcatcgaac tggaccaccg cgacctgcgg caaaaatgcc tgtccgagat cgagaaggtc
     1801 cagtggatcc cagcctgggg caaggaccgc atccgcaaca tggtggaggg gcggaccgac
     1861 tggtgcattt cccgccagcg ctcctggggc gtgcccatca cggcggtcta ttgcggggcc
     1921 tgcggccaag tggtgggcga ccccaagacc ctggcggcca ccgtggccct ggtggaaaaa
     1981 gaggggatcg acggttggtt cggccatggg cccgaggcgg tcctgccggc ggggtccatc
     2041 tgcgcctgcg gctcgaagga cttcatcaag gaagaggaca tcctggacgt ctggttcgag
     2101 tccggcagct cccatttcgc ggtcctggac gagcgggaag agctgcggga aagccccggc
     2161 cgtccctccc gcttcgtgct ttacctggaa ggcagcgacc agcaccgcgg ctggttccag
     2221 catgccctct ggaccggggt ggccctgaag ggccgggcgc ccttcagcgg ggtgctcacc
     2281 cacggctggg tgctggacgg cgagggcaag gccatgcaca agagcgcggg caacgtcatc
     2341 gatcccctgg agatggtgaa aaagtacggg gcggacgtgc tgcggctttg ggtgtcctcg
     2401 gagaactaca ccgaagatgt gggcatcagc gaggagatgc tcaaccgcat gtcggaggcc
     2461 tatcggcgca tccgcaacac cttccgcttc atcctgggca acctattcga cttcgacccg
     2521 gtgaataata gtgttgcgga tgatcagttg ttagatattg acgcttgggc caaaatggcg
     2581 gcaaatggaa atttgcactt aatcaatgga tattatgaag agtatcaatt tactaaaatt
     2641 tttcatcaga ttgataattt ttttgccgtt gaattgagtt ctaaatattt cgacattctt
     2701 aaggaccgtc tttatacgtc ggaaaaaaat tcgaagaaca gaaggtctgc gcaaacagtt
     2761 ttgtacaaaa tattaaaaga ttatgttgtt gctattgctc ctattctttc gttcactgcg
     2821 gaagaaattt ggcagtcaat gcccgaaagc tatcggtcgg aaaaaagtgt ttttttatca
     2881 tcgtttcccg attttgaagc ggaaatagcc gaactatgcg tttcgagaag agttaatgcc
     2941 gtcttgtatt tggatgagtg ggcaagaatt ttctat
//
LOCUS       DASTJJ010000469         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_797719, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000469 DASTJJ010000000
VERSION     DASTJJ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797719"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="sppA"
                     /locus_tag="VFR02_05735"
     CDS             complement(<1..690)
                     /gene="sppA"
                     /locus_tag="VFR02_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9869979.1"
                     /translation="MKPTPPPSTPPASGSRTFAYLLAGLGFFFLLTVAVVAVSVTHLV
                     GHLDGGSGGSSEITLIEINGPIDDGDDVVRRIHRFRKGDSKALLLRLNSPGGAVAPSQ
                     EIYDEVLKARKAKKIVVASMSSLAASGAYYIASAADRIVADPGTLTASIGVIAEFPDA
                     SSLLDKVGVKFETIKSGKFKDTGSFSRAMTPAERAYLQGTIDDVFGQFVSSVLDARRD
                     VFQKKLAPSLGL"
     gene            complement(741..1187)
                     /locus_tag="VFR02_05740"
     CDS             complement(741..1187)
                     /locus_tag="VFR02_05740"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9869980.1"
                     /translation="MNNIRIRVAGIFVKDGKILLVRHEKNGKSYWLLPGGGAEYGESM
                     EEALQREFREEVGLDIQVGSMVLAHDSIPPDRHRQVLNLYFLVSSSGGELKVTPDAVL
                     RDAAFHPLADFAGLTVHPDVKGPILEGLATDWKAGLRYLGNQWKEA"
     gene            complement(1239..1688)
                     /locus_tag="VFR02_05745"
     CDS             complement(1239..1688)
                     /locus_tag="VFR02_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9869981.1"
                     /translation="MATKSKLLRMDNVGIVVESFDEVIPFFTELGLILEGRAAIEGEW
                     AGRVTGLGSQRVEIAMMATPDGHSRIELSRFLAPPIVADHRNAPVNALGYLRAMFTVS
                     DVDETLARLRKYGAQLVGEVVQYEDTYRLCYIRGPEGLLIGLAEQLK"
     gene            complement(1741..2085)
                     /locus_tag="VFR02_05750"
     CDS             complement(1741..2085)
                     /locus_tag="VFR02_05750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET9869982.1"
                     /translation="MKIKKFNVYLADLSPRFGTETGKTRPVVILQTNLLNDAGHPSTV
                     VCPITTQVVRGSDILRVHLSKTEVSLKRDSDILVDQIRAIDNRRFVRHLGELTQAHKQ
                     KLADNLKVLLFE"
     gene            complement(2082..>2299)
                     /locus_tag="VFR02_05755"
     CDS             complement(2082..>2299)
                     /locus_tag="VFR02_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869983.1"
                     /translation="IKGDVFEDVEAITKQLKIPRNAYINEALDFYNEFNRRKSLRKKL
                     RHESLLTRKSSMEVLSELEKLEENLQE"
BASE COUNT          431 a          666 c          707 g          495 t
ORIGIN      
        1 gaggcccagg ctgggggcca gcttcttttg gaagacgtcc cggcgggcgt ccaacacgga
       61 ggaaacgaac tggccgaaaa cgtcgtcgat cgtcccctgc aggtaggccc gctcggcggg
      121 cgtcatggcg cggctgaagg acccggtgtc cttgaacttg ccgctcttga tggtctcgaa
      181 cttgacgccc accttgtcca gcagggacga ggcgtcgggg aactcggcga tgacgccgat
      241 ggaggccgtc agggtgcccg ggtcggccac gatgcggtcg gcggcggagg cgatgtagta
      301 ggcccccgag gccgccaggc tggacatgct ggccaccacg atcttcttgg ccttccgggc
      361 cttcaggacc tcatcgtaga tctcttggga gggcgcgacg gccccgccgg ggctgttcaa
      421 gcgcagcaac agggccttgg agtcgccctt gcggaagcgg tggatgcggc gcaccacgtc
      481 gtccccgtcg tcgatggggc cgttgatctc gatgagcgtg atctcgctgg aaccgcccga
      541 tcccccgtcc aggtggccca ccaagtgggt cacgctgacg gccaccacgg ccacggtcaa
      601 taggaagaaa aagcccaatc cggccagcag ataggcgaag gttcgggacc cggaagcggg
      661 cggagtgctg ggcggggggg tgggtttcat ggaaaaggca tgttatcaga caagcggggg
      721 tgatgcaaga aacccttgtt tcaagcctct ttccactggt ttcccagata gcgcagcccg
      781 gccttccagt ccgtggccag gccctccagg atcgggccct tgacgtcggg atgtacggtg
      841 agccccgcga agtccgccaa gggatggaaa gcggcgtccc gcagcacggc gtcgggggtg
      901 accttcaatt ccccaccgga ggaagagaca agaaagtaga ggttcagcac ctgccggtgc
      961 cggtcgggcg ggatggaatc gtgcgccaag accatcgagc cgacttggat gtccaggccc
     1021 acttcctcgc ggaactcacg ttgaagggcc tcctccatcg attcaccgta ctcagccccc
     1081 ccgccgggca agagccagta gctcttgccg ttcttctcat ggcgcaccag gaggatcttg
     1141 ccgtccttga cgaagatccc cgccacgcgg atccggatgt tgttcatgag gcctcccccc
     1201 tggacaaggt ggaattcatt tttttcttct tttttcgatc atttgagctg ttcagccagt
     1261 ccgatgagaa gtccttcggg accgcgtatg tagcaaagcc gatacgtgtc ttcgtactga
     1321 acgacttcgc cgacgagttg ggcgccgtac ttgcggagcc tggcgagcgt ctcgtcgaca
     1381 tcgctcaccg tgaacatggc gcgtaggtag ccgagggcgt tcaccggagc gttccgatga
     1441 tcggcaacga tgggcggggc gagaaaccgc gagagctcga tccggctgtg gccgtcgggg
     1501 gtggccatca tggcgatctc cacgcgctgg gagcccagcc cggtgacacg cccggcccat
     1561 tctccctcga tcgcggcccg cccttcgaga atcaggccta gttcggtgaa aaaagggatg
     1621 acctcatcga acgattccac cacgatcccg acgttgtcca tccttagcaa cttgcttttg
     1681 gtggccatgt tttccggtct ccgtttctct catctgctcc gggtccctgt cgtggacaag
     1741 ctattcgaac aataggacct ttaggttatc ggccaacttt tgtttatggg cctgggtcaa
     1801 ctcaccgaga tggcggacaa accgccgatt gtcgatggcc cggatttggt caaccaggat
     1861 atccgagtcc ctcttgaggg aaacttccgt ttttgagagg tggacccgaa ggatgtcgga
     1921 gccacgaacg acttgagtgg tgattgggca aaccacggtg ctcggatgac ctgcatcgtt
     1981 cagcaaattc gtttggagga tcaccaccgg cctggttttt ccagtttcgg tcccaaacct
     2041 cgggctcaag tcggcaagat agacgttgaa tttcttgatc ttcattcctg gaggttttct
     2101 tccaattttt ccaattccga caggacttcc atggagcttt tccgggtcag taaggattca
     2161 tggcggagtt ttttcctgag gcttttacgc cgattaaact cgttgtaaaa gtccaaagcc
     2221 tcgttgatgt aggcgtttcg gggaattttg agttgtttcg tgatggcttc gacatcctcg
     2281 aagacatcac ctttgatct
//
LOCUS       DASTJJ010000470         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_797788, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000470 DASTJJ010000000
VERSION     DASTJJ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797788"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFR02_05760"
     CDS             complement(<1..392)
                     /locus_tag="VFR02_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869984.1"
                     /translation="MGRPAKRPDRFFPALPWAFAAAAWLPLAAQAPALVHRLACPQQV
                     QQEEAVNVEFGRVLLRGERLYPDIRVGGPYLHCSYPPLFPFLESLLMRLTPDPWAPGR
                     SLAFAGFLACGLMLGGAVWRRWRDPAWA"
     gene            complement(395..1678)
                     /gene="cysN"
                     /locus_tag="VFR02_05765"
     CDS             complement(395..1678)
                     /gene="cysN"
                     /locus_tag="VFR02_05765"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HET9869985.1"
                     /translation="MEVFLEAERAKDLLRFSTAGSVDDGKSTLIGRLLYDSKGVFEDQ
                     LDAIRRTTEKKGGEDIDFALITDGLSAEREQGITIDVAYRYFVTAKRKFIIADTPGHE
                     QYTRNMVTGASTADLAIILIDARNGVLIQSKRHGYIAKLLGIPHLVVAVNKMDLVDFS
                     QEVFERIKKDYTDFFSQLVAENPAAPSLQNLEFIPLSALKGDNVTVKSPAMPWYGGKS
                     LLQYLETVEIARDRNLDDFRFPVQLVLRPNLDYRGFAGKVVSGVAKKGQKVVTLPSGA
                     VSTLKAIDTFDGERDEAWPPACVTLRLADEIDISRGDMLALPGNEPKCEKQFEANLCW
                     MVKEPMALKKKYVLKLATGEVKAMVSAIRWVRDMRTLDKVEAQTLALNDIARVVVQTL
                     KPICFDPYARNRATGSFILIDELTNNTVAAGMITE"
BASE COUNT          286 a          545 c          579 g          302 t
ORIGIN      
        1 aaggcccagg ccggatcgcg ccaacggcgc cagaccgccc cgcccagcat caggccgcag
       61 gccaggaaac cggcgaaggc gagcgagcgc ccgggcgccc aaggatcggg cgtgagacgc
      121 atgaggaggg actccaaaaa aggaaaaagc ggcggatagg aacaatgcag gtagggcccg
      181 cccacgcgga tgtccggata gagcctctcc ccgcgcagga gcacgcggcc gaactccacg
      241 ttgacggctt cttcttgttg gacctgttgg ggacaggcca ggcggtggac aagggccggg
      301 gcctgggccg ccagcggcag ccaggcggcg gccgcgaagg cccacggcag cgccggaaaa
      361 aatcggtcgg ggcgcttcgc ggggcggccc accgctattc cgtgatcatc ccggcggcga
      421 ccgtgttgtt ggtgagctca tcgatgagga tgaagctgcc cgtggcccgg ttccgggcgt
      481 aggggtcgaa acagatgggc ttcagggtct gcaccaccac ccgggcgatg tcgttgaggg
      541 ccagggtctg ggcctccacc ttgtccaggg tcctcatgtc ccgcacccac cggatggccg
      601 acaccatggc cttcacttcc cccgtggcca gcttcaggac gtatttcttc ttcaaggcca
      661 tgggctcctt caccatccag cagaggttgg cctcgaactg cttctcgcac ttgggctcgt
      721 tgcccggcag ggccagcatg tcgccccgcg aaatgtcgat ctcgtcggcc aggcgcaggg
      781 tcacgcaggc gggcggccag gcctcgtccc gttccccgtc gaaggtgtcg atggccttga
      841 gggtcgagac cgcccccgag ggcagggtca ccaccttctg gcccttcttg gccacgcccg
      901 ataccacctt gcccgcgaaa ccgcggtagt ccaggttggg ccgcagcacc aactgcacgg
      961 ggaagcggaa atcgtccagg ttgcggtccc gggcgatctc cacggtctcc aggtattgca
     1021 gcaggctctt gcccccgtac cagggcatgg ccgggctctt cacggtgacg ttgtccccct
     1081 tcagggccga cagggggatg aactccaggt tctgcagcga gggcgcggcc gggttctcgg
     1141 ccaccagttg ggagaagaag tccgtgtagt ccttcttgat cctctcgaag acctcctggc
     1201 tgaagtccac caggtccatc ttgttgaccg ccaccaccag gtgggggatg cccagcagct
     1261 tggcgatgta gccgtggcgt ttggactgga tcagcacccc gttgcgcgcg tcgatgagga
     1321 tgatggccag gtccgccgtg gaggcgcccg tcaccatgtt gcgggtgtac tgctcgtggc
     1381 ccggggtgtc ggcgatgatg aacttgcgct tggccgtgac gaagtagcgg taggccacgt
     1441 cgatggtgat gccctgctcg cgctcggcgg aaagcccgtc ggtgatcagc gcgaagtcga
     1501 tgtcctcgcc gcccttcttc tcggtggtgc ggcggatggc gtccaattgg tcctcaaaga
     1561 cgcccttgga atcgtagagc aggcgcccga tgagggtgga cttaccgtcg tccacgcttc
     1621 cggcggtcga gaagcgaagc aggtccttgg cccgctcggc ttcgagaaat acctcaattt
     1681 caaacgcagc tttttcttcg agggtctggg tg
//
LOCUS       DASTJJ010000471         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1656068, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000471 DASTJJ010000000
VERSION     DASTJJ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656068"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFR02_05770"
     CDS             complement(<1..349)
                     /locus_tag="VFR02_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET9869986.1"
                     /translation="MSKTTPAPAAPANNDKRTKILRGLLAVGLLAAAYFGYQTFRYEG
                     TDDAYVEAHTVMLAPRIGGVVEKVLVKENEKVKAGQVLAELDAQDYATAYDQAAADAE
                     AVRADLAAAAQDYQ"
     gene            complement(342..>1625)
                     /locus_tag="VFR02_05775"
     CDS             complement(342..>1625)
                     /locus_tag="VFR02_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9869987.1"
                     /translation="VGGEVPGGLTLDEAQQEAVTASPAYGQAQAEEREASWGQVEAFS
                     DGFLPHVSVTGRHLFDNRYPFLNIQFAGPPTLQFPSISPDTSLSLDASFDLFDGFQNL
                     HKLDAANNSHQAAKIQADWAQFQLGEQVRLDFYKAIAAQELADMADQNVKTLQDHLRI
                     VNEQMQNGQATKYDSLRVKVQLDEAQSDQISADDKVVLTREELARVMGLKMDDRPLSG
                     TLPVLDPQALLKQVADDAYLGRPDLRAADYQARAAADQSAASQASFLIPKVTLIGEYQ
                     WYDSPDYLNGVVTHTDSFRDDYYIGAAASWNLFDGASALARAAEAGEKAQAARERFRA
                     AEVQAPYDFDLWKRKLVSSAALYRAKLTDVDEAKESARLATVGFQAGTRTTTDVLDAE
                     LEEYRAAEGLVQAQIDSLEALVNLELTIGKRLDHE"
BASE COUNT          220 a          549 c          550 g          306 t
ORIGIN      
        1 gctggtaatc ctgcgcggcc gccgccaggt cggcccgcac ggcttccgcg tcggccgccg
       61 cctggtcgta ggcggtggcg tagtcctggg cgtccaactc ggccagcacc tggcccgcct
      121 tcaccttttc gttctccttc accagcacct tttccaccac cccgccgatg cggggcgcca
      181 gcatgacggt gtgggcctcc acgtaggcgt cgtccgtgcc ctcgtagcgg aaggtctggt
      241 agccgaaata ggccgccgcc agcaggccca ccgccagcag gccgcgcagg atcttggtgc
      301 gtttgtcgtt gttcgccggg gccgcggggg ccggggtcgt cttactcatg gtcaagcctc
      361 tttccgatgg tcaactcgag gttcaccagc gcctcgaggg agtcgatctg ggcctggacc
      421 aggccctcgg ccgcgcggta ttcctccaac tcggcgtcca acacgtcggt cgtggtgcgg
      481 gtccccgcct ggaaccccac ggtggccagt cgcgcgcttt ccttggcctc gtccacgtcc
      541 gtcagcttgg cccggtaaag ggccgcgctg gaaaccagct tccgcttcca caagtcgaag
      601 tcatagggcg cctggacctc ggcggcccgg aaacgctccc gggcggcttg ggccttttcg
      661 ccggcctcgg cggcgcgggc cagggccgag gccccgtcga agaggttcca gctggcggcg
      721 gccccgatgt aatagtcgtc ccggaagctg tccgtatggg tcacgacgcc gttcaggtaa
      781 tccggcgagt cgtaccattg atactcgccg atgagcgtca ccttggggat gaggaacgag
      841 gcctggctgg ccgcgctttg gtcggccgcc gcgcgggctt ggtagtccgc ggccctcagg
      901 tccggccggc ccaggtaggc gtcgtcggcc acctgtttca acagggcctg ggggtccagc
      961 acggggagcg tgccgctcaa gggacggtcg tccatcttca accccatgac ccgcgccagc
     1021 tcctcccggg tcaacaccac cttgtcgtcg gccgagatct ggtcggactg ggcctcgtcc
     1081 agctgcacct tgacccgcag ggagtcgtac ttggtggcct ggccgttctg catttgttcg
     1141 ttcacgatgc gcaggtggtc ctgcagggtc ttcacgttct ggtcggccat gtcggccagt
     1201 tcctgcgcgg cgatggcctt gtagaagtcc agccgcacct gttccccgag ctggaactgg
     1261 gcccagtcgg cctggatctt ggccgcctgg tggctgttgt tggccgcgtc cagcttatgc
     1321 aggttctgga acccgtcgaa aaggtcgaag ctggcgtcca aactcaagga cgtgtccggg
     1381 gaaatgctgg ggaactgcag ggtgggaggc ccggcgaatt ggatgttcaa gaaggggtag
     1441 cggttgtcga acaggtgccg gcccgtgacg ctcacgtggg gcaggaagcc gtccgagaag
     1501 gcctccacct gcccccaaga agcttcccgt tcctcggcct gggcttggcc gtaggccggg
     1561 gaggccgtca cggcctcctg ctgggcctcg tccagcgtga ggccgccggg cacttccccg
     1621 cccac
//
LOCUS       DASTJJ010000472         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_311132, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000472 DASTJJ010000000
VERSION     DASTJJ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311132"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFR02_05780"
     CDS             <1..697
                     /locus_tag="VFR02_05780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9869988.1"
                     /translation="DYRRQILSGKATFADLAKKYSQDPGSSAQGGELGWVTRGTLVKE
                     FEDQIFDHLKKGQISEPFKTQFGYHLVQLEDYEHSYTSTFDQVRSKVLDQYKSDKAQD
                     EVTALARQLAIKLKNKESLAKAAGELSLKSSVTPWFTGSGGIPGIAHSSAVTPDLAEL
                     YVGDWKGPLPIGGSRYYFQITQAKERPLSGKALEKASAEAVRRLGEHKQDLWVRSFLD
                     DQRKKLKVKTYLN"
     gene            complement(690..1262)
                     /locus_tag="VFR02_05785"
     CDS             complement(690..1262)
                     /locus_tag="VFR02_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HET9869989.1"
                     /translation="MPRLVLASTSPRRRRILAFLGIPFQAAAPLGVIEERRRGESPRA
                     LAVRLALEKALSLRARYPERWILGADTLVVLGDRVFGKPKGPAQLRRTLSLLSGRRHQ
                     VLTGMVLLSPKGGRYVGVGESRVTFRALDQGEMTAYARTREPYDKAGGYAIQGTAAGW
                     VRRLEGDYFNVMGLPANLLLETLAASGWIS"
     gene            complement(1273..1527)
                     /locus_tag="VFR02_05790"
     CDS             complement(1273..1527)
                     /locus_tag="VFR02_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HET9869990.1"
                     /translation="MAAKNAGMLILFALLGAVLGGYVGELLGLLTGPGFLHDLFTRSF
                     DVGLDTLVLDLKVVVLTFGLKIYINLCSILGMIAGLYYAR"
     gene            complement(1531..>1751)
                     /locus_tag="VFR02_05795"
     CDS             complement(1531..>1751)
                     /locus_tag="VFR02_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HET9869991.1"
                     /translation="FQRTQKAAPRYEIVREWGPDHNRSFEAACVMAGKPLGRGTGKSK
                     KEAEQLAAQAALQALGVPVPGAATGQEA"
BASE COUNT          329 a          608 c          550 g          264 t
ORIGIN      
        1 ggattaccgc cggcagatcc tctccggcaa ggccaccttc gccgacctgg ccaagaaata
       61 ttcccaggac ccgggctcca gcgcccaagg cggcgagttg ggctgggtga cccggggcac
      121 cctggtgaag gaattcgagg accagatctt cgaccacctg aagaagggcc agatctccga
      181 gcccttcaag acccagttcg gctaccacct ggtgcagttg gaggactacg agcattccta
      241 caccagcacc ttcgaccagg tgcgttccaa ggtgttggac cagtacaagt cggacaaggc
      301 ccaggacgag gtgaccgccc tggcgcgtca attggccatc aagctgaaga acaaggaatc
      361 cctggccaag gccgcgggtg aactgtccct gaagtcctcc gtcacgccct ggttcacggg
      421 ctcgggcggc atcccgggga tcgcccattc cagcgccgtc acgcccgacc tggccgaact
      481 ctatgtgggc gattggaagg gccccctgcc catcggcggc agccgttatt acttccagat
      541 cacccaggcc aaggaacggc ccttgtccgg caaggccttg gaaaaggcca gcgcggaagc
      601 ggtgcggcgg cttggggaac ataaacagga cctgtgggtc cgcagcttcc tcgacgacca
      661 gcgcaagaag ctgaaggtga agacctacct caactgatcc aaccggaggc ggccagggtc
      721 tccagcagca ggttggcggg caggcccatc acgttgaaat agtccccctc cagccgccgg
      781 acccagcccg ccgcggtccc ctggatggcg tagccgcccg ccttgtcgta gggctcccgg
      841 gtgcgcgcgt aggccgtcat ttcgccctga tccaaggccc ggaaggtcac ccggctttcc
      901 cccaccccga cgtacctccc ccccttcggg gacaacaaca ccatccccgt caagacctgg
      961 tgccgccttc cgctcaaaag cgagagggtc cgccgcagtt gcgccgggcc tttgggtttc
     1021 ccgaaaaccc ggtcccccaa caccaccaac gtgtccgcgc ccaggatcca gcgttcggga
     1081 tagcgggcgc gcagggaaag ggccttctcc agggccaacc ggaccgccaa ggcccggggg
     1141 gattcccccc tccggcgttc ctcgatcacg cccaagggcg cggcggcttg gaaggggatg
     1201 cctaaaaagg cgaggatccg gcggcggcgg ggcgaggtgg acgccaggac caggcggggc
     1261 atcccggccg ccttaacggg cgtaataaag gccggcgatc atccccagga tggaacagag
     1321 gttgatgtag atcttcaggc cgaaggtcag caccaccacc ttgaggtcca gcaccagggt
     1381 gtccaggccc acgtcgaaac tgcgcgtgaa caggtcgtga aggaatcccg gccccgtgag
     1441 caatcccaac agctcgccca catagccccc caacaccgcc cccaggaggg cgaagaggat
     1501 gagcatcccg gcgttcttgg cggccatggg tcaggcctct tggcccgtgg cggcgccggg
     1561 caccggcacc cccagggcct ggagcgccgc ctgggcggcc aattgctcgg cttccttctt
     1621 gctcttgccg gtcccccgtc ccaaaggctt gcccgccatc acgcaggcgg cctcgaaact
     1681 gcggttgtgg tccggccccc attcgcgcac gatctcgtag cggggggcgg ccttctgggt
     1741 gcgctggaaa t
//
LOCUS       DASTJJ010000473         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_311143, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000473 DASTJJ010000000
VERSION     DASTJJ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311143"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VFR02_05800"
     CDS             complement(<1..1385)
                     /locus_tag="VFR02_05800"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET9869992.1"
                     /translation="MKFKMPSIFSAASFLLDGVAVALAFLAANWIRFRSGWFPVHSVT
                     AYHHFRSFVFLLVCLYWVVFKYFGLYRKRRGISGVDELSKIIKASLVAGFLVGTAVFL
                     THFTGFSRLVAALMAPGVVLLAWAERLALRRVQVFLRRRGVGVTRVLVVGTGETARVL
                     LQRLRANPGLGYRVVGVVAETGSGRMVEGYKVLGRLEQFSRILRQGRPEEVIFALPAS
                     AHAKLIPMLVTLQDTWVRYKIVSDLFGIITNPLASDVLFDMPVFEMKEAPLSAWRCRA
                     LKRSFDFALSLLGILLLAIPLGALALGVKLSSPGPVFFRQKRVGRDGKIFHMLKFRSM
                     RPGSETMAFTAKDDPRVTPFGKFIRKTSLDELPQLFNVLRGEMSLVGPRPEVPGLVEK
                     FEKEIPRYFERHQVKSGITGWAQVNGLRGNTSLSERVKYDIYYIENWSLLFDVKIILR
                     TVLDILEHQHA"
BASE COUNT          343 a          518 c          483 g          236 t
ORIGIN      
        1 taggcatgct ggtgctccag gatgtccaag accgtcctca ggatgatctt cacgtcgaag
       61 agcaggctcc agttctcgat gtaatagatg tcgtacttca cccgctcgga aagcgaggtg
      121 ttcccccgca ggccgttcac ctgggcccag cccgtgatgc ccgatttcac ctggtggcgc
      181 tcgaaataac gggggatctc cttctcgaat ttctcgacca agccgggcac ctcgggccgg
      241 gggcccacca ggctcatctc gccccgaagc acgttgaaga gctggggcag ttcgtccagg
      301 ctggtcttgc ggatgaactt tccgaaaggc gtcacccggg ggtcgtcctt ggccgtgaag
      361 gccatcgtct cgctgccggg gcgcatggaa cggaacttga gcatgtggaa tatcttccca
      421 tcccggccca ccctcttctg gcggaaaaag acggggccgg gcgaggagag cttgacgccc
      481 agggccagcg cccccagggg gatcgccagc aggaggatcc ccagcaacga cagggcgaaa
      541 tcgaacgacc gcttcaaggc gcggcagcgc caggcgctca agggggcttc cttcatctcg
      601 aagaccggca tgtcgaacaa cacgtcgctg gccagcgggt tggtgatgat gccgaagagg
      661 tccgacacga tcttgtagcg cacccaagtg tcctgcaggg tcaccagcat ggggatgagc
      721 ttggcgtggg cggaggccgg cagggcgaag atgacctcct cgggccgccc ctgccggagg
      781 atgcgggaga attgttccaa cctccccagg actttatagc cctcgaccat ccggcccgat
      841 ccggtttccg ccaccacccc gaccacccgg tagcccagtc ccgggttcgc ccgcaggcgt
      901 tgaagcagga cccgggcggt ctcccccgtc cccacgacca acacccgggt caccccgacg
      961 ccccggcgcc gcaagaagac ctggacccgc cgcaacgcca aacgttccgc ccaagccaac
     1021 agcaccaccc cgggggccat caaggccgcc accaaccgcg aaaaaccggt gaagtgggtc
     1081 aggaagacgg cggtgcccac caagaacccc gccacgaggg aggccttgat gatcttggaa
     1141 agctcgtcca cgcccgaaat gccccggcgt ttgcggtaaa gaccgaaata cttgaagacg
     1201 acccaataaa ggcataccag caggaagacg aaggaacgaa aatgatggta ggcggtgacc
     1261 gaatggaccg ggaaccagcc cgaacggaac cggatccagt tggcggccag gaaggccagg
     1321 gcgaccgcca cgccgtccaa caggaaactg gcggccgaga agatggatgg catcttgaat
     1381 ttcataagcc ccttgcgcgg accgtcttcc ggaaccgggc ggtcattata acccagctcc
     1441 cgtgggtgga ataccttttg gagggcgcgg gcccatgata aaatccaaaa accgcggccc
     1501 cgaacaggcc ggccatcccg aaaggcaggc ggcatgcacc ccaacttctt cattatcggc
     1561 gcgccgaaat gcggcacctc
//
LOCUS       DASTJJ010000474         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1410821, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000474 DASTJJ010000000
VERSION     DASTJJ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410821"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VFR02_05805"
     CDS             <1..1493
                     /locus_tag="VFR02_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HET9869993.1"
                     /translation="NPLLNKFFQIPLVRFLASVKLALPMLLILAALLAAGTIVESLYT
                     ASLAKRFVYDTWWFTAFLALLGLNVLLSALSRFPWKKHQIGFVITHLGILCVLAGSFL
                     TQRWGTDGQIAVAEGGQGSIFQEDKPILTCQVGSRTVRRIPAGFPNLEPRPSFPFSYR
                     IGDDARLTVDRFYLNARRLVGAKPAGPGEKGSAAVRLKLESSFVQEADWLFLGRQGQD
                     RVDLGPASVFFLSEGDWKSKSDREKNGLPPNALVLIERPQGDWLMKTRHRGAWAPAKA
                     LALGQSLPTGWMDMKATVTQALSRAVPDVSYAPVPLAAQEPQPALRFTARSGGQVAES
                     WVGYEDQVTFTLNSKPWRVTYGPRQAPLPFSLALQKFHLGFDPGTQNPASYVSDVEAQ
                     DLATGEKFPAVIRMNQPLHYRGYTVYQASYFPMEDGRYTSVFSVGRDPGLVLKYAGAL
                     VMVLGIIFMFWFKNPAWKKREKDEEKPLETPGDGGPAAGAPGGPRG"
     gene            1420..3066
                     /gene="ccsA"
                     /locus_tag="VFR02_05810"
     CDS             1420..3066
                     /gene="ccsA"
                     /locus_tag="VFR02_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9869994.1"
                     /translation="MRKNPWKHLAMAALLLAPLAARAAETFDFSPLAALPLQAGGRVK
                     PLETFARESVRAITGRESFQGRPALALLFDWIAKPDDWGSKDILLVGDLELRSKLGLP
                     AGQERVSPDLLLKDEGFLTFAKAAYAKQGEKAALDPAEKESLALFQRLRRFEDIESGD
                     ALALIPVPDSTRWISVGELRRAYEKAPKKMPPVAQALLLRFAQVLQAYAEGSPSDLTA
                     ACLQWKALLEPVQAGPQVQPWRLGLEVAYDRFKPFRWAWILHLAAALCLALALLPGAR
                     RLFPVGAGFFGAGVLVSALGFTARCLIAGRPPVSNMYESIIWVTFGMTVFALVFSLVL
                     KNRSFLLAGSILAVIGFVLADNVPSVLDPSIQPLEPVLRSNFWLTIHVLTITLSYAAF
                     LLSLGIGHLGLWTLWKTPGDKDRLKLRAQWTYRVVQVGVVLLAAGTILGGVWASYSWG
                     RFWGWDPKETWALIALLGYVAILHGKVAGWLKDRGFLLGVVCAFLGVLMAWYGVNFVL
                     GVGLHSYGFSKGGLPYVCTYVALELGWVAAVAVKSRGKTV"
     gene            3063..3722
                     /locus_tag="VFR02_05815"
     CDS             3063..3722
                     /locus_tag="VFR02_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9869995.1"
                     /translation="MKLVLTGATGTAGSKALEAALADKRVKQVTVLTRRMLATRHAKL
                     KVVLMKDFSDYSKVKKDLKGQDACLWCLGISQNAVPEDEYIRITYDYALAAAKALKAA
                     NKKFTFCFLSGDGAATSEKSPMLFGRVKGKTENALGKVGFTRLYHFRPGYIHPTKDQP
                     KKLVYERLFEPLTPLLHRFVPSMIISSADLGKAMVQAACAGAPKTILDNLDILKLAKK
                     N"
BASE COUNT          580 a         1203 c         1236 g          704 t
ORIGIN      
        1 gaaacccgct tttgaacaaa ttcttccaga tccccctcgt gcgcttcctg gcgtccgtca
       61 agttggccct gcccatgttg ctcatcttgg cggcgctcct ggcggcgggg accatcgtgg
      121 aatcgctcta tacggcttcc ctcgccaagc gtttcgtcta tgacacctgg tggttcacgg
      181 ctttcctggc gctcttgggc ttgaacgtgc tcttgtcggc cctgtcgcgg ttcccctgga
      241 aaaaacacca gatcggcttc gtcatcaccc acctgggcat cctctgcgtg ctggcggggt
      301 ccttcctcac ccagcgctgg gggaccgacg ggcagatcgc cgtggccgag ggcggccaag
      361 gctccatttt ccaggaggac aagcccatcc tgacctgcca ggtgggatcc cggacggtgc
      421 ggcgcatccc ggcgggattc cccaacctgg agccccggcc ttccttcccc ttctcctacc
      481 ggatcgggga tgacgcccgg ctgacggtgg accgttttta cctgaacgcc cggcgcctgg
      541 tgggggccaa gcccgcgggc cccggggaaa agggctctgc ggcggtgcgg ttgaagctcg
      601 aaagttcctt cgtgcaggag gcggattggc ttttcctggg ccgccagggc caggaccggg
      661 tggacctggg cccggcctcg gtcttctttc tgtccgaggg ggattggaag tccaagagcg
      721 accgggaaaa gaacgggctt ccccccaacg cgctggtgtt gatcgagcgt ccccaggggg
      781 actggctgat gaagacccgc caccgggggg cctgggcccc ggcaaaggcc ctggccttgg
      841 gccagtccct tcccacgggc tggatggaca tgaaggccac cgtcacccag gccctgtccc
      901 gcgccgtgcc cgacgtttcc tacgcgcccg tccccctggc cgcgcaggaa ccccagccgg
      961 cgctgcgttt caccgcgcgg tcgggcggcc aggtggccga gtcctgggtg gggtacgagg
     1021 accaggtgac ctttaccctc aacagcaagc cctggcgggt gacctacggc ccccggcagg
     1081 cgccgctgcc tttttccctg gcgctccaga agttccactt ggggttcgac cccggcacgc
     1141 agaacccggc ttcctacgtc agcgacgtgg aggcccagga cctggcgacg ggggaaaaat
     1201 tccccgcggt gatccgcatg aaccaacccc tgcattaccg gggctacacg gtttaccagg
     1261 ctagttattt ccccatggag gatggaagat atacgtccgt cttctcggtg gggcgggacc
     1321 cgggcctggt cttgaagtac gccggggcgc tggtgatggt gttgggcatc atcttcatgt
     1381 tttggttcaa gaatccggct tggaaaaaga gggagaagga tgaggaaaaa cccctggaaa
     1441 cacctggcga tggcggccct gctgctggcg cccctggcgg cccgcgcggc tgagaccttc
     1501 gacttttcgc ccctggcggc cctgcccctg caggccggcg ggcgggtgaa acccctggag
     1561 accttcgcgc gcgaaagcgt ccgcgccatc acgggccggg agtccttcca gggccgtccg
     1621 gccctggcgc tgctctttga ttggatcgcc aagcccgacg attgggggtc caaggacatc
     1681 ctgttggtgg gcgacctgga actgcgctcc aaactgggcc tgccggcggg ccaggagcgc
     1741 gtctcgcccg atctcctgct caaggatgag ggcttcctca ccttcgccaa ggcggcctac
     1801 gccaagcagg gggagaaggc cgcgctggac ccggccgaaa aggaaagcct ggcgctgttc
     1861 cagcgcctgc ggcgcttcga ggacatcgag tccggcgacg cgctggccct gatccccgtg
     1921 cccgattcca cgcgttggat ctcggtgggc gaactgcgcc gggcctacga gaaggcgccg
     1981 aaaaagatgc cgcccgtggc ccaggccctg ctgctgcgct tcgcccaggt gctgcaggcc
     2041 tacgccgaag ggagcccctc ggacctcacg gcggcctgcc tgcaatggaa ggccctgctc
     2101 gaacccgtcc aggccggccc ccaggtgcag ccctggcggt tggggttgga agtggcctat
     2161 gaccgattca agcccttccg ctgggcctgg atcctgcacc tggccgccgc cctgtgcctg
     2221 gccttggccc tgttgccggg ggcccgacgg ctctttccgg tgggcgcggg ctttttcggg
     2281 gcgggggtgc tggtgagcgc gctgggcttc acggcccgct gtctcatcgc cgggcggccg
     2341 ccggtgtcca acatgtacga atccatcatc tgggtcacct tcggcatgac ggtcttcgcc
     2401 ctggtctttt cgctggtcct caagaaccgg tccttcctct tggcgggttc catcctggcg
     2461 gtgatcggtt tcgtgctggc cgacaacgtg ccttcggtgc tggatccctc catccagccc
     2521 ctggagccgg tgctgcgcag caacttctgg ctgaccatcc acgtgctcac catcacgctc
     2581 agctacgcgg ccttcctgtt gtccttgggc atcggccatc tgggactatg gaccctttgg
     2641 aaaacccccg gggacaagga ccggctcaag ctccgggccc aatggaccta ccgggtggtg
     2701 caggtgggcg tggtgttgct ggcggccggc accatcctgg gcggggtctg ggccagttat
     2761 tcctggggcc ggttctgggg ttgggacccc aaggagacct gggccctgat cgccctcttg
     2821 ggctatgtgg ccatcctgca cggcaaggtg gcgggttggc tgaaggacag gggcttcctg
     2881 ttgggcgtgg tctgcgcctt cctgggcgtg ctcatggcct ggtacggggt caatttcgtg
     2941 ctgggcgtgg ggctgcacag ctacggcttc tcgaagggcg ggttgcccta cgtctgcacc
     3001 tatgtggcgc ttgaactggg atgggtcgcg gccgtggcgg tgaagtcgcg gggaaagacg
     3061 gtatgaagct ggtcctgacc ggggcgacgg gcaccgccgg gtccaaggcc ctggaagcgg
     3121 ccctggcgga caagcgggtg aagcaggtga ccgtgctcac gcgccggatg ctggcgacca
     3181 ggcacgccaa actgaaagtg gtcctgatga aggacttttc cgattattcc aaggtgaaga
     3241 aggacctgaa gggccaggac gcctgcctct ggtgcctggg gatctcgcag aacgctgtgc
     3301 cggaggatga gtacatccgc atcacctacg attacgccct ggcggcggcc aaggccctga
     3361 aggcggcgaa caagaagttc accttttgtt tcttgagcgg ggatggggcg gccaccagcg
     3421 aaaaaagccc catgcttttt ggccgcgtca aggggaagac cgagaacgcc ttgggcaagg
     3481 taggcttcac ccggctttac catttccgcc cgggctacat ccatcccacg aaggaccaac
     3541 ccaagaagct ggtctatgag cgcctgttcg agcccctcac gcccctcctg caccgtttcg
     3601 tccccagcat gatcatctcc tcggcggact tgggcaaggc catggtccaa gcggcctgcg
     3661 ccggggcgcc gaagaccatc ttggataatt tggatatcct aaaattggcg aaaaagaatt
     3721 aac
//
LOCUS       DASTJJ010000475         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1657196, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000475 DASTJJ010000000
VERSION     DASTJJ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657196"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3234)
                     /locus_tag="VFR02_05820"
     CDS             complement(<1..>3234)
                     /locus_tag="VFR02_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869996.1"
                     /translation="GFIPHATPTPVFTLTPTGTSTPTVTSTVTATPTETPTNNGGFTS
                     TWTASPTETETPQLQDTDTPTETPSPTPTASGDAYGSWQEAVDVAGFPGKGGLRSLWF
                     NNRLWALGGYYSNGSIFQLDNSVWSSPDGVQWAQESPKASFPGRADFGAVVFDPSTGS
                     NNSGRMWVLGGLGLTDSLNDVWSSTDGVHWLQVAATTPWSPRWGLTATVFQGQIWVIG
                     GVDEGNNALSDAWHSPDGVNWTQAPAPPFAARGGHQTLVYGGQLWVLGGQGTGARSLN
                     DVWSSPDGTTWTEQTSAAEWPAREFFCAQVLDGQMVVASGETNDGRFLADLGDVFHSG
                     DGIHWTRSTYEAPFGVRSQAACASDGSRIWILGGETPASSKNLLSDVWYSNGPGIPSP
                     TPTPTPTPDELGQWTRVNPDAGFIPRDNPALASFNGRLWMAGGTNDFIGPVGDVWSSA
                     DGVNWSLAAQESIFEEQDDPILLSLNGKLWALNMVDWSTNSQAVYSSPDGINWTETTQ
                     AAPYGAPQEAYSFGGKLWIVDYTQNLQAGTSRVYSSADGANWAMVGTTPGGGRQGAAY
                     TVFNGKMWIFGGTNPQNNTTYYEVLNSTDGATWNEVSNQTPMTILRDYDVVATSDRIY
                     LIRKDGGDQWSTPDGIHWTQVAGPPAFAPRSNFSTTAYNQRFWILGGGDTNGDQSSDI
                     WTAPGNDSGLSTWTPTPSPTSTWTPTPTGTISLTPQPTATPTPSLTWTPVPTPTIPQV
                     SGRDWAPSLRPNPVFSKRKYEASAWFKDQLWVIGGDSGSGPLADTWSSPDGSNWGAVT
                     TQAPWGAEEGQAAVSFQGQLWVMGGNNGQSDINGIWNTLDGQTWTMGSPVPWTARSFF
                     PAVVFNGQLWVLGGSGKIDVWSSADGSTWIRQPDGPFTGNGGQAVVFNGRIWFIGAGQ
                     AGAPGAIWSTSDGISWDEPVTVEPFESRTGFTATVADGRIWVLGGKAASSGLKSDVWY
                     SCDGVNWHEATAQAAFSPRQGLLALPFQPDPGTNSDALWVLGGLDYSNQSNGNAWYTP
                     LLGHDQPCYAPTPTPTPTPLSGAYSVWNLGTGQAAFAPRADQGAAVFD"
BASE COUNT          571 a         1010 c         1067 g          586 t
ORIGIN      
        1 gtcgaacacg gcggccccct ggtcggcccg gggagcgaag gcggcttggc ctgttcccag
       61 gttccacacg ctataagccc cggacagggg ggtgggggtc ggggtggggg tcggggcgta
      121 acagggctgg tcgtggccca ggagcggcgt ataccaggcg ttcccgttcg attggttgga
      181 gtaatccaac ccgcccagca cccacaaggc gtccgaattc gttcccgggt cgggttggaa
      241 aggaagggcc aagaggccct gccgggggct gaaagccgcc tgggcggtcg cttcatgcca
      301 gttcacgcca tcgcatgaat accaaacatc gctcttcaac ccggaggaag cggctttccc
      361 gcccaggacc cagatccgcc cgtccgcaac cgttgccgtg aatcccgtac gcgattcgaa
      421 gggttccacc gtgaccggtt catcccagct gatgccatcg gaggtggacc agatggcccc
      481 cggcgcgccc gcctgccccg ccccgatgaa ccagatccgg ccattgaaaa cgacggcttg
      541 gccgccattg ccggtgaagg gtccgtcggg ctggcggatc caggtcgatc catccgcgga
      601 ggaccagaca tcgatcttcc ccgatccgcc caacacccag agttgcccgt tgaagacgac
      661 cgccgggaaa aagctacgtg cggtccaggg caccggggac cccatggtcc aagtctgtcc
      721 gtccaacgta ttccaaatac cgttgatgtc cgattggccg ttgttgccgc ccatgaccca
      781 aagctggccc tggaagctga cggcggcctg cccctcttcc gcgccccagg gtgcttgcgt
      841 cgtgaccgcc ccccaattcg acccgtcagg cgaggaccag gtatccgcca atgggccgga
      901 accggaatcc ccgccgatca cccagagttg gtccttgaac caggcggagg cctcatattt
      961 ccttttactg aagaccggat tgggcctcaa ggagggtgcc cagtcccggc cggaaacctg
     1021 cgggatcgtc ggggtgggga cgggcgtcca ggtcaagctt ggggtcgggg tagctgtggg
     1081 ctgcggcgtc aagctgatgg tcccggtggg ggttggggtc caggtggaag tggggcttgg
     1141 cgtcggggtc caggtggaaa ggcccgaatc attcccggga gcggtccaaa tatcgctcga
     1201 ttggtccccg ttggtgtccc caccacccaa gatccagaat cgctggttgt aagccgtcgt
     1261 ggaaaaattc gatcgtgggg cgaaggccgg cggcccggcc acctgtgtcc aatggatgcc
     1321 gtcgggggtg ctccactggt ccccgccatc cttgcggatc aggtagatgc ggtccgacgt
     1381 ggccacgacg tcataatccc tcaagatggt catgggcgtc tggttggaga cctcgttcca
     1441 agtggcgccg tcggtggaat tcaggacctc gtaataggtc gtgttgttct gggggttggt
     1501 gcctccgaag atccacatct ttccgttgaa gaccgtataa gcggcgcctt ggcggccccc
     1561 gcccggggtc gtccccacca tggcccaatt cgccccatcg gcggaggagt agaccctgct
     1621 ggtgccggct tgcaggttct gcgtatagtc cacgatccag agtttcccgc cgaacgaata
     1681 ggcttcctgg ggggcgccgt agggggcggc ttgggtggtt tcggtccaat tgatcccatc
     1741 cggcgaactg tagaccgcct gggaattcgt actccaatcc accatgttca gggcccagag
     1801 cttgccgttc aaggacagga ggatggggtc atcctgttcc tcgaaaatgc tttcctgggc
     1861 ggccagggac cagttgacgc cgtcagcgga ggaccagaca tcccccaccg gaccgatgaa
     1921 atcgttggtc cctcccgcca tccagagccg tccgttgaag gaagccaacg ccgggttgtc
     1981 cctcgggatg aaccccgcat cgggattcac ccgcgtccac tggcccaact catccggggt
     2041 cggggtggga gtgggggtgg gcgaaggtat ccctggacca ttggaatacc agacatccga
     2101 aagaaggttc ttggacgacg ccggggtttc cccgccgagg atccagatcc ggctcccgtc
     2161 ggatgcgcag gccgcctgag aacgcacccc gaaaggcgcc tcgtaggtcg aacgggtcca
     2221 gtggatcccg tcccccgaat ggaacacgtc gccgaggtcg gccaggaacc gtccgtcatt
     2281 cgtttccccg gaagcgacca ccatctgacc gtccaagacc tgggcacaga aaaattcccg
     2341 tgccggccat tcggcggccg aggtctgctc ggtccaggtc gtgccgtccg gggagctcca
     2401 aacatcgttc aaggaccggg cgccggttcc ctgtcccccg agcacccaca attggccccc
     2461 atacacgagg gtttggtgtc caccccttgc cgcaaagggg ggcgcggggg cctgggtcca
     2521 gttcaccccg tcgggggaat gccaggcgtc cgataaggca ttgttcccct catcaacccc
     2581 tccgatcacc cagatctggc cctggaacac ggtggccgtc aagccccagc ggggcgacca
     2641 gggagtggtg gcagccacct gaagccagtg gaccccgtcc gtcgagctcc atacgtcgtt
     2701 caggctgtcc gtcagcccca acccccccag gacccacatg cggccggagt tattggaacc
     2761 ggtggaaggg tcaaagacca cagcgccgaa atcggcccgg cccgggaaag acgctttggg
     2821 tgattcctgg gcccattgga cgccgtcagg ggaactccaa acgctgttgt ccagttggaa
     2881 tattgaaccg ttggaataat atccccccag cgcccagagt cggttgttga accagaggga
     2941 acggagcccc cctttgcctg ggaatcccgc cacatcgacc gcttcctgcc aactgccgta
     3001 ggcatccccg ctggcggtcg gggtggggct gggggtttcg gtcggggtat ccgtatcctg
     3061 tagttgtgga gtttcggttt cggtcgggga agccgtccag gtggaagtga acccgccgtt
     3121 gttggtgggg gtttcggtgg gcgtggcggt gaccgtcgag gtcacggtcg gggtcgaggt
     3181 cccagtgggg gtcaaggtga agacaggcgt gggggtggca tgggggatga aacc
//
LOCUS       DASTJJ010000476         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1411890, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000476 DASTJJ010000000
VERSION     DASTJJ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411890"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFR02_05825"
     CDS             complement(<1..293)
                     /locus_tag="VFR02_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869997.1"
                     /translation="MRARDLRQDLKQLKAEIGGNTRTHKEFGCSSTPAPTPPGSVEMT
                     RKAGVGAGILNNQVAAGAGLEGVRVKTKDEHRGKPPVVSLTGRVGFSTPFPES"
     gene            complement(290..976)
                     /locus_tag="VFR02_05830"
     CDS             complement(290..976)
                     /locus_tag="VFR02_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9869998.1"
                     /translation="MGGRSDTYYYLNRPALAYLSKTARILVSTEIRSAHINTFEREHD
                     KRVLAMRIQIENEGGLEGLTWLSDYEMRCGLKMAWKKALETGRGWDLAGAKLHRVHQR
                     TPDGFFTGRLAGKDYAFVLEYEHTPYNRDKMTALVLNLTRDFPTALRLIVSRDKDHAV
                     RLAHGLEGFLKGDPQALPLWGFTFFEKVAALPFCRVPWVTLKGGYLPFVKDPILTTPN
                     PKDGQEEATA"
     gene            1037..2199
                     /locus_tag="VFR02_05835"
     CDS             join(1037..1301,1301..2199)
                     /locus_tag="VFR02_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611339.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET9869999.1"
                     /translation="MARKGHSAEQIISILRQVEVEQSKGRSVEEACREAQVTAHTYYR
                     WRKEYGGLKVDQAKYLKELEKENARLKRLVADLTLDKQMLQEVAKGKLLSPAKLRRAA
                     QMLIDMFRVSQRRACRVLAQPRNTHRYIPQIRLDEPPLTARMIQLACMYGRYGYRRIT
                     AMLNWEGWRVNHKRIERLWREAGLKVPQKQPKRRRLWFNDGSCMRLRAQKPNHVWSYD
                     FVAERTTDTRAFRILTVIDEFTHECLALPVARKFRAHDIQGILSELFLERGIPDFIRS
                     DNGPEFVAKELKDWLPKLGVKTAYIEPGSPWENGYNESFNGKLRFDLLDGEIFDTLWE
                     ARVILERWRREYNTRRPHSSLRQLPPAPEAWILEPKTKPMPWLGSTNPVYGVT"
     gene            2262..2639
                     /locus_tag="VFR02_05840"
     CDS             2262..2639
                     /locus_tag="VFR02_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870000.1"
                     /translation="MITNKLPSTADSTLTAANDLSNAGGVKSAWDASVALGLSGALNP
                     IKYAPGTLEEMMSGTSRRLIIVEVVINGISHFVVVTGEVYDSAKKMCRFTVADPGYNV
                     QYLDQYDDVIHNKSISTIRGFLP"
     gene            2636..3502
                     /locus_tag="VFR02_05845"
     CDS             2636..3502
                     /locus_tag="VFR02_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870001.1"
                     /translation="MRTKALTRAGYFVLFFASAGFSEADIPVGYVWSQNLVDIYGDVP
                     PALIFIQDKNGSRTGGNPDLPVDAYGRQGGTLDGLDEIPLSSVEQVNIGSDDPADQGQ
                     PQPTTGWTIHLLDGGSQTYRVNLVGLKTGMSEITVTQYIAPPAAHRKFQSMIDALVSQ
                     GVTTQLDLTIDAQTGSISSTRVASSKTLFQDVQTACQMGLITSNRACDFLTRQARLID
                     QALDKDRDEEARAQIRVFLHALGYSRPFGCRDEDHHGEVKQPALGILQEDAKALLDQV
                     GKPDRHFGEKRR"
     gene            complement(3571..4191)
                     /locus_tag="VFR02_05850"
     CDS             complement(3571..4191)
                     /locus_tag="VFR02_05850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9870002.1"
                     /translation="MKKIKCLALAALVAGWGTFAQAAAVGKAAPDFQAVDTQGKAVSL
                     KALKGHYVVLEWHNQDCPFVKSQYKGKMQKLQGKWTARGVTWISVISSAPGKEGSVDA
                     AKANADVKETSSQVSATVLDPTGKIGRAYGAKSTPHMFVIDPKGTLIYDGAIDNDPLD
                     DAVSDKTQDGGAYVNYVDQALTQSLAGEKVSTPTTAPYGCGVKYAH"
     gene            complement(4221..>4428)
                     /locus_tag="VFR02_05855"
     CDS             complement(4221..>4428)
                     /locus_tag="VFR02_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870003.1"
                     /translation="GALFFPLHPNQIENAAPQEFRYSSHVVHLDLKRSDQLTGDVQTL
                     EGLVLLKSKAGSKAYQIAVPLGPR"
BASE COUNT          920 a         1222 c         1364 g          922 t
ORIGIN      
        1 atactttcgg ggaaaggtgt ggaaaacccc acccgtcccg tcagggacac cacggggggt
       61 tttccacggt gttcgtcttt cgtcttaact cttactcctt cgagtcctgc acccgcagcc
      121 acttggttgt tcaagatgcc agcccccact cccgctttcc tggtcatctc tactgatcca
      181 gggggagtgg gggctggcgt cgaagaacat ccaaactcct tgtgggtgcg ggtgtttcca
      241 ccgatttcgg ctttaagctg ttttaagtcc tgccttaagt ccctagccct catgccgtgg
      301 cctcttcctg cccatccttc gggtttgggg tcgtcaggat ggggtccttc acaaagggaa
      361 ggtatccgcc cttcagggtg acccaaggca cccggcagaa gggaagagcg gccaccttct
      421 caaagaaggt gaagccccaa aggggcaaag cctgggggtc gcccttgagg aagccttcca
      481 agccatgggc caggcggacg gcgtggtctt tgtcccggct gacgatgagc ctcaaggcgg
      541 tggggaagtc ccgcgtcagg ttcaggacca gggcggtcat cttgtcccgg ttgtaggggg
      601 tatgttcgta ttcaaggacg aaggcgtaat ccttgcccgc cagcctcccg gtgaagaacc
      661 cgtcaggggt cctttggtgg acccggtgga gcttggcccc ggccaggtcc cagccccttc
      721 ccgtctccaa ggccttcttc caggccatct tgaggccgca ccgcatttcg tagtccgaga
      781 gccaggtcag cccttccagg cctccttcgt tctcgatttg gatacgcatg gccaggaccc
      841 gcttgtcgtg ctcccgctca aaggtgttga tatgggcgga acggatctca gtggaaacca
      901 atatccgggc cgtcttggat aggtaggcca gggccgggcg gttgaggtag tagtaggtgt
      961 ctgacctgcc ccccaaaacc tggtccagaa ataaagtcac agttggcttt agaatgggcc
     1021 tacaaagggg gccaatatgg cacgcaaggg acatagcgct gagcagatca tttccatctt
     1081 gagacaggtg gaggtggagc agtcgaaggg aaggtcggtc gaggaagcct gtcgggaagc
     1141 ccaggtcacc gcccacacct actaccgttg gcgcaaggaa tatgggggcc tgaaggtcga
     1201 ccaggccaag tacctcaaag aactggaaaa agagaacgcc aggctcaaac gcctggtggc
     1261 cgacctcacc ctggacaaac aaatgctcca ggaggtggcc aagggaaact tctaagcccg
     1321 gccaagctgc gccgggcggc ccaaatgctc atcgatatgt ttcgagtgtc ccagagaagg
     1381 gcctgccggg tgctggccca accacggaac acccaccgct acatacccca gatcaggcta
     1441 gacgagccgc ctttgacggc ccggatgatc caactggcct gtatgtatgg ccgctatggg
     1501 taccggcgga tcaccgcgat gctcaactgg gaaggctggc gggtgaatca caagcgtatt
     1561 gaacgcctgt ggagggaagc ggggctgaaa gtcccgcaaa aacagccaaa aaggcgaagg
     1621 ctgtggttca acgacgggtc ctgcatgagg cttcgggccc agaagcccaa ccatgtgtgg
     1681 agctacgact tcgtggcgga gaggaccacc gataccaggg ccttcaggat actcacggtg
     1741 atcgacgagt tcacccatga gtgcctggcc ctgccggtgg cccggaagtt ccgggcccac
     1801 gacatccagg ggatcctgtc ggagttgttc ctggaaaggg gtataccgga cttcatccgg
     1861 tcggacaacg ggcctgagtt tgtagctaaa gaactgaagg actggctgcc gaagttgggg
     1921 gtgaagacgg cctacatcga gccggggagc ccctgggaga acggctacaa cgagagcttc
     1981 aacgggaagc tccggttcga tttgctggat ggggagattt tcgataccct ctgggaggcg
     2041 agggtgatac tggagagatg gcggagggag tataacacca ggaggccgca cagctccctg
     2101 agacaacttc cgccggcgcc ggaagcttgg attttggagc ctaagaccaa gcctatgccg
     2161 tggctgggat caacaaaccc ggtctatggt gtgacttaat aagtggtgca aacaacgggg
     2221 gcaggtcagc caaaattggt tgttatgtga ccagtatggc tatgatcacg aacaagttgc
     2281 cttctacggc cgattctact ttgaccgccg caaatgattt gagcaatgcg ggtggggtca
     2341 aatcggcttg ggatgcgtcg gttgccttag gactttctgg ggctttaaac ccgatcaaat
     2401 atgcccctgg cacacttgaa gaaatgatgt ccggcacgtc ccgaaggctc atcattgtcg
     2461 aagtcgtgat caatggcatt tcccactttg tcgttgtgac gggagaggtt tatgattccg
     2521 cgaaaaaaat gtgtcgattc actgttgcgg atcccggcta taacgttcag tatttggatc
     2581 aatatgacga tgtaatccac aataaatcga tatcaacgat aagggggttc ttgccatgag
     2641 aacaaaagca cttacccggg ccggctattt cgtgcttttc tttgcctctg cgggattttc
     2701 cgaagctgat attcccgttg gatatgtttg gagtcaaaat ttggtcgaca tctatggtga
     2761 tgttccgcca gctttgatct ttattcagga taaaaatgga agccggacgg gcggaaatcc
     2821 cgacttgccc gttgatgcct atgggcgcca aggcggaact ttggatggat tggatgagat
     2881 ccccttgtcc agtgttgagc aggtaaatat cgggagcgac gatccggcgg accaggggca
     2941 accgcaaccg acaacgggat ggaccattca tttgttggat ggcggttcgc aaacgtaccg
     3001 cgtcaattta gtcggtttaa aaacggggat gagcgaaata accgtcacac aatatattgc
     3061 cccgccggcg gctcatcgga aattccaatc gatgatcgat gccttggtat cgcagggtgt
     3121 cacaacccaa cttgatctga ccatcgacgc gcaaaccggg tctatctcca gcacaagggt
     3181 tgcatcgagc aaaacccttt ttcaagacgt ccaaaccgcc tgccagatgg ggctcatcac
     3241 gtccaaccgg gcctgcgact tcctgacccg gcaggccagg ctcatcgatc aggcgttgga
     3301 caaggaccgg gatgaagaag cgagggccca gatcagggtt tttctccacg ccctgggcta
     3361 ttcccggccc ttcgggtgtc gggacgagga ccaccacggc gaggtcaagc agccggcctt
     3421 aggcatcctg caggaggacg cgaaggccct gttggaccag gtggggaagc ccgaccgtca
     3481 ctttggggag aagcgtcggt aaagatccct ggaagtccct gaaataaaaa aagcccccgg
     3541 gttttcccgg gggctttttg gtgcaagggc tcagtgggcg tatttcaccc cgcaaccgta
     3601 gggcgcggtg gtgggggtgg acaccttttc ccccgccagg gactgggtca aggcctggtc
     3661 cacgtaattg acgtaggcgc cgccgtcctg ggtcttgtcc gagaccgcgt cgtccagcgg
     3721 gtcgttgtcg atggcgccgt cataaatgag cgtgcccttg gggtcgatga cgaacatgtg
     3781 gggcgtggac ttggccccgt aggcgcggcc gatcttgccc gtcgggtcca ggacggttgc
     3841 ggaaacttgg gaactggtct ccttcacatc ggcgttcgcc ttcgcggcgt cgaccgaccc
     3901 ctctttcccc ggtgccgagg agatgacgga gatccaggtg acgcccctgg ccgtccactt
     3961 gccctgcagc ttctgcatct tgcccttgta ctggctcttg acgaaggggc agtcctggtt
     4021 gtgccattcc agcaccacgt aatgcccctt cagggccttc aggctgacgg ccttgccttg
     4081 ggtgtcgacg gcctggaagt cgggggcggc tttccccacg gccgccgctt gggcgaaagt
     4141 cccccagccg gccaccagcg cggccagggc caggcacttg atctttttca tgttcccctc
     4201 ctttgaaagt gtgacgggcg tcaacgcggg cccaagggca cggcgatctg gtaggccttg
     4261 gaccctgcct tggatttcag caggaccaac ccttccagcg tttggacgtc ccccgtgagc
     4321 tggtcggacc gtttgaggtc caagtggacc acgtgggacg agtagcggaa ttcctgcggc
     4381 gcggcgttct cgatctggtt gggatgcagg gggaaaaaga gggccccc
//
LOCUS       DASTJJ010000477         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1534907, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000477 DASTJJ010000000
VERSION     DASTJJ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534907"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFR02_05860"
     CDS             <1..1087
                     /locus_tag="VFR02_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9870004.1"
                     /translation="NRLEEAGTAPLEDLFARVSAVVAARLEPYADTSLAPFAREVVAL
                     DHADLDPVARRLPPLQAFPKGDSRLLPGQFAGLFDLRRQQWRKVRFIADVNQNEKVTA
                     REIIAALPEECLILCDLGFFGFAWFDDLTARKQFYVSRLRQKTSYTPIHTFYQDADLF
                     DGIVWLGAYRADRAQHAVRLVRVRHRGRWHTYLTNVLDPTLLAVREVLTLYARRGDFE
                     LAIDLVKQHLGLHLLWSAKDVVIQQQLWAVLLISQILQALRLEVAGRAGVDPFEVSLE
                     LLARWFPRLVAEGKDPIATFVTYGRQGGFIRPSSRLKIDAPTIPKENLCPLPPDLVLE
                     RVPHHSQRKCGKRPEKRVRKQPNKQAN"
BASE COUNT          176 a          394 c          313 g          225 t
ORIGIN      
        1 caatcggttg gaggaggccg gcaccgcccc tctggaagac ctctttgccc gggtctcggc
       61 cgtggtggcc gcgcgccttg agccctacgc cgacacctcc ctggccccct tcgcccgcga
      121 agttgtcgcg ttggatcacg ccgacctgga cccggtggcg cgccgcctgc ctcccttgca
      181 ggcttttccc aagggggatt cccgcctgct cccgggccaa ttcgctggcc tcttcgacct
      241 gcgccgccaa cagtggcgca aggttcgttt cattgccgac gtgaaccaaa acgagaaggt
      301 cactgcccgg gagatcatcg ccgccttgcc cgaagagtgc ctgatcctct gcgatttggg
      361 cttcttcggc tttgcgtggt ttgacgatct caccgcccgc aagcagttct acgtctcccg
      421 cctgcggcag aagaccagct acacgcccat ccatactttc taccaggatg cggacctgtt
      481 cgatggcatt gtttggttgg gggcctaccg ggcggatcga gcccaacacg ccgtccgctt
      541 ggttcgggtc cgccaccgcg gccgctggca cacctacctc accaatgtcc tggatccgac
      601 cctcctcgcg gtgcgtgagg ttctcaccct ctacgcacgc cgcggggact tcgagttggc
      661 catcgatttg gtcaagcagc acctgggttt gcacctgctc tggtcggcca aggacgtcgt
      721 gatccaacaa caactctggg cggttctcct catcagccag atcctccaag ccctccgctt
      781 ggaagtcgcc gggcgggccg gtgtcgatcc ctttgaggtg tccctggagt tgctcgcccg
      841 ctggttcccg cgcctggttg ccgagggcaa ggatcccatt gccaccttcg tcacctacgg
      901 acggcagggg ggcttcatcc gcccctcctc ccgcctcaag atcgacgccc cgaccatccc
      961 caaagagaat ctgtgtccat tgccgcccga cctggtcctg gagcgcgttc cgcaccattc
     1021 ccagcgcaag tgtggcaagc gacccgagaa acgcgtccgt aaacaaccga ataagcaggc
     1081 caattagttg gagtcaatgg cctccgtt
//
LOCUS       DASTJJ010000478         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1534922, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000478 DASTJJ010000000
VERSION     DASTJJ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534922"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFR02_05865"
     CDS             complement(<1..567)
                     /locus_tag="VFR02_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870005.1"
                     /translation="MDALQQATPHVKKNIFLIRMVLALALGCLAFFYLQANPGQKPMV
                     LGYVSFLGLSFLPFGWWEETRFRAVRFQYLIFAMDLAFLLGALYLFERFDTGLLIMMF
                     LTLFISALGQSPGRSLVVALAVASLYGYLLYVQHPDFNYLDPGFPLSCALIFVVALHS
                     GYLAYRTVQEEKDMLALARRIDLLTEKVR"
     gene            632..1546
                     /locus_tag="VFR02_05870"
     CDS             632..1546
                     /locus_tag="VFR02_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9870006.1"
                     /translation="MKSPRSAYPPSVWAALLSVYVVWGSTYLAIRFAVGAIPPFLMGG
                     TRFLAAGAILYAVLRAAGMPRPRAAHWRSTALIGFLLIAVANGGVCWAEQKVPSGVSC
                     LLVATVPIWMVLLDWLWKGNGRPGPRLWAGLLLGFGGTAWLGLSGGEVSGGDPARVLG
                     LAAFPLAWAVGSLYARSAPLPASPLMATALEMLWGGVFQLLAGLVTGEFSQMGPIPAL
                     AAWSWVYLTLVGSLVGFTSYIWVLQRSNPALASSYAFVNPVIAVLLGWVFAGEPLTLP
                     VAGATALIVAGVVLITRANPRRARRRVG"
     gene            1965..>2362
                     /locus_tag="VFR02_05875"
     CDS             1965..>2362
                     /locus_tag="VFR02_05875"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9870007.1"
                     /translation="MIQAENKVTPLVVPSATTKTAKAMWRKFTRDEQIDWGGSIAFFG
                     VHVIGVFAIFTGISWAAVAMCFLMYYVRMFAITGVYHRYFSHRTYKTSRLFQFMLAFL
                     GCSCGQQGPLWWAAHHRHHHKYSDTEEDIHS"
BASE COUNT          394 a          749 c          745 g          474 t
ORIGIN      
        1 ccgcaccttc tcggtcaaaa ggtcgatgcg ccgggccagg gccagcatgt ccttttcctc
       61 ctggacggtc cggtaggcca ggtagcccga atgcagggcc accacgaaga tgagcgcgca
      121 ggacaagggg aaacccgggt ccaggtagtt gaagtccgga tgctggacgt aaagcaggta
      181 accgtaaagc gacgccacgg ccagggccac caccaggctt cggcccggcg actggcccag
      241 ggccgagatg aaaagcgtga ggaacatcat gatgagcagg ccggtgtcaa agcgctcaaa
      301 gaggtaaagg gcccccagca ggaaggccag gtccatggcg aagatcaggt actggaacct
      361 gacggcccgg aaccgggtct cttcccacca cccgaagggc aggaaggaca accccaagaa
      421 ggacacatac cccaagacca tgggtttttg gcccgggttg gcctgcaggt agaaaaaggc
      481 caagcatccc agcgccaggg cgagcaccat acggatgagg aagatgtttt ttttgacgtg
      541 gggcgtagcc tgctgaaggg cgtccatgtt agcctcggtc cgtccggatt tgtcgcgagc
      601 ctcatcccat catatgacaa gggtgccgaa cgtgaaaagc cccaggtccg cttacccccc
      661 cagcgtctgg gcggcgctcc tctcggtcta cgtggtctgg ggctccacct acctggccat
      721 ccgtttcgcg gtgggggcca tccccccctt cctcatgggc ggcacccgct ttttggccgc
      781 cggggccatc ctctacgccg tcctccgggc ggcagggatg ccgcgccccc gggcggccca
      841 ttggcgctcc accgccctca tcggtttttt gctcatcgcg gtggccaacg gcggcgtctg
      901 ctgggccgag caaaaggtcc cctcgggggt ctcctgcctg ttggtggcca ccgttcccat
      961 ctggatggtg ttgttggact ggctctggaa aggcaacggc cgtccgggtc cgcggctttg
     1021 ggccgggctc ctcttgggtt tcggcggtac ggcttggctg gggctttcgg gcggggaggt
     1081 gtcgggcggc gacccggcgc gggtgttggg actggcggct ttccccctgg cctgggccgt
     1141 gggttcgctc tacgcgcgct cggcgccgct ccccgcctca cccctgatgg ccaccgcctt
     1201 ggagatgctc tggggcgggg tcttccaatt gctggccggc ctcgtgaccg gggaattttc
     1261 ccagatgggg cccatcccgg ccttggccgc ttggtcctgg gtctacctga ccctggtggg
     1321 gtcgctggtc gggttcactt cctatatctg ggtattgcaa aggtccaacc cggccttggc
     1381 ctccagttat gccttcgtca atccggtcat cgcggtcctg ctgggatggg tcttcgcggg
     1441 ggaacccctg accctgccgg tggcgggggc cacggccctc attgtggcgg gtgtggtgct
     1501 catcacccgg gccaaccccc gccgggcccg ccgtcgcgtg ggatgagctt ccgcgccgga
     1561 tcgggttttc ccggatagag ccccattttt tcccactttt tgcccaggtt tagcgttcgg
     1621 atttttgtac ccgatgaaag tgggttgcgg taatatgaaa gaggacgctc cccaagggtt
     1681 tttggggggc tctttgtcgg tcccaaggcc tgaattaaac caaaattcaa ggtttttgtc
     1741 cctccgggga gggggctgac ttgtgctcgg agaagtccta ccttaacggg gatggggccg
     1801 ggccgtttag aaccaagaaa tccaaccgct cgggtccgcc ggtcaggggc ccggggaaat
     1861 actcacccta ttgtcggccg ggtccgccgc gcatcctgaa catgttgccg caggccgagt
     1921 cccgccccaa taacaacaaa tcacttgaac gaggtgtcgt accaatgatc caagctgaaa
     1981 acaaagtcac tcccctggtg gtgccgtcgg cgacgacgaa gaccgccaag gccatgtggc
     2041 gcaaattcac ccgcgatgag cagatcgact ggggcggcag catcgccttc ttcggcgttc
     2101 acgtcatcgg cgttttcgcc atcttcaccg gcatcagttg ggccgccgtc gccatgtgtt
     2161 tcctcatgta ctacgtgcgc atgttcgcca tcacgggcgt gtaccaccgt tacttttccc
     2221 accgcaccta taagaccagc cgcctgttcc agttcatgct ggccttttta ggctgctcct
     2281 gcggccagca ggggcccctg tggtgggccg cccatcaccg ccatcaccac aagtattccg
     2341 acaccgagga ggacatccac tc
//
LOCUS       DASTJJ010000479         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_190265, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000479 DASTJJ010000000
VERSION     DASTJJ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190265"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFR02_05880"
     CDS             complement(<1..644)
                     /locus_tag="VFR02_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9870008.1"
                     /translation="MKIGIIGSGVVGQTLGKKLVELGHDVVLGTRDPKKLDEKKNMAG
                     SLNEWLAAVKHQAKVATFKEAAAHGELLISCTHGQISVEALQQAEAGTVGAKTLIDTA
                     NELDFSKGFPMALASEARSVSEAIQKAFPNLKVVKSLNTIAAPIMVAPGMLKGGDHTI
                     FVAGDDAGAKAQVTDLLKSFGWTDILDLGGLPAAKGPEMYMGLWVRLYGSLKNG"
     gene            complement(730..1038)
                     /locus_tag="VFR02_05885"
     CDS             complement(730..1038)
                     /locus_tag="VFR02_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870009.1"
                     /translation="MAFQKVAAAFAKDPAVGRKRVFASENVLTVKGKIFAMISSKGEF
                     VVKLPRERVEGLVQARKGKRFEPGPGRVMKEWLAVKAPPRAWPALAQEAYDHVKKGQS
                     "
     gene            complement(1120..1722)
                     /locus_tag="VFR02_05890"
     CDS             complement(1120..1722)
                     /locus_tag="VFR02_05890"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HET9870010.1"
                     /translation="MNPVLRMDRGFWNLAAADLAPLAGYFLWHWLPVDLVFLFWVETL
                     FLFLMALAAALFGPKPPNFTNILGGTLFAGWFLLIFLMFVCALDPRMDDMEKRYPFLV
                     LPRLFWQDNLWPQVSLLAGSYLYQAVTALLRPEPGDLVLRDLLAPAERLVILFLSIFL
                     GLLTLRLFPDYGGVFLMLLALKGSLDIGQYWRRPPAKTLE"
     gene            complement(1719..2597)
                     /locus_tag="VFR02_05895"
     CDS             complement(1719..2597)
                     /locus_tag="VFR02_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9870011.1"
                     /translation="METHQLRYFLAVSQTGRFTAAAQACHVSQPSLSIQVAKLEGELG
                     GALFERGRKGARLTARGEAFLPRARAILDQMEAAQEDAAALSGLRLGKVALGCMPTTG
                     AHLLPPILTAFKKAHPTIQVQLKEESSPDLAQGLEQGEIELAILDEAGLRPSLGHQAV
                     LTEELLLALPPKHPLAGKKALALKQVAPDPFILMKTGHGFRQITLELYRKEGLEPRVV
                     FESGGIETVQALVAAGLGISLVPRMVARFPGVAYAALSSPKAQRTLSLAWRQKAPFSP
                     AAQALRRVIFSVLGGR"
     gene            2695..>3045
                     /locus_tag="VFR02_05900"
     CDS             2695..>3045
                     /locus_tag="VFR02_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9870012.1"
                     /translation="MAKGTLYNKVWDAHTVDKLPTGQTQLFIGLHLIHEVTSPQAFDM
                     IAEKGLKVAYPARTFATVDHIVPTQDQARPFADGEAEEMMGAIEKNVRQFGVKFFDFK
                     SGDQGIVHIVGPEQG"
BASE COUNT          566 a          999 c          977 g          503 t
ORIGIN      
        1 atcccgttct tgagggatcc atagagccgc acccacaggc ccatgtacat ctcggggccc
       61 ttggcggcgg gaaggccgcc caggtccagg atgtcggtcc aaccgaagga cttgagcagg
      121 tccgtcacct gggccttggc acccgcgtcg tcgcccgcca cgaagatcgt gtggtccccg
      181 cctttgagca tgcccggggc caccatgatg ggcgcggcga tggtgttgag gctcttgacc
      241 accttcaggt tggggaaggc cttctggatg gcctccgaca cgctgcgcgc ctcgctggcc
      301 agggccatgg ggaacccttt gctgaagtcc agctcgttgg cggtgtcgat cagggtcttg
      361 gcccccaccg tcccggcctc ggcctgctgc agggcctcca ccgagatctg gccgtgggtg
      421 cagctgatga ggagctcgcc gtgggcggcg gcttccttga aagtcgcgac cttcgcctgg
      481 tgcttcacgg cggccagcca ctcgttgagc gaccccgcca tgttcttttt ctcatccagc
      541 tttttggggt cccgggtgcc cagcaccacg tcatggccca gttccaccag ctttttcccc
      601 agggtctgcc ccaccacgcc cgaaccgatg atgccgatct tcatgacggc ctcccgcctt
      661 ggaatgaaaa cggctcaatc ctaggcaggc ccgggaccgg attgcaaggg aaggcctggg
      721 cgccgaaggt cagctttgcc cttttttcac gtggtcgtag gcttcctggg ccagggccgg
      781 ccaggcccgg ggcggggcct tgaccgccag ccattccttc atgacccggc cggggcccgg
      841 ttcgaaccgt ttccccttcc gcgcttggac cagcccctcc acccgttccc gcggcagctt
      901 caccacgaac tcgcccttgg aggagatcat ggcgaagatc ttccccttca cggtcagcac
      961 gttctcggag gcgaagaccc gttttcggcc caccgccgga tccttcgcga aggcggccgc
     1021 caccttttga aaggccattt ccgcggaagg ctttggtttt ttatcctggg tcatggggcc
     1081 attttaacgg cgcgcggccc aaaagacatc cggccccgct cattccaagg tcttggccgg
     1141 gggccggcgc caatactggc cgatatcgag ggagcccttg agggccaaaa gcatgagaaa
     1201 gaccccgcca tagtcgggga acaggcgcag ggtgaggagg cccaggaaga tggacaggaa
     1261 aaggatgacc agccgttcgg cgggcgccaa gaggtcccgc aggaccaggt ccccgggctc
     1321 gggccgcagc agggccgtca ccgcctggta aaggtaggaa ccggccaaga gggacacctg
     1381 gggccagagg ttgtcctgcc aaaagagccg ggggagcacc aggaagggat agcgtttttc
     1441 catgtcatcc atccgcgggt ccagggcgca gacgaacatc aggaagatga gcaggaacca
     1501 acccgcgaaa agggtgccgc ccaggatgtt ggtgaagttc gggggtttgg ggccgaagag
     1561 ggccgctgcc agggccatga ggaaaagaaa aagggtctcg acccaaaaaa ggaacaccag
     1621 gtccaccggg agccaatgcc agaggaaata gcccgccagg ggcgccaggt ccgccgccgc
     1681 caagttccaa aaacccctgt ccatccgcaa aaccgggttc atcgcccccc caggaccgag
     1741 aaaatgaccc gacgcaaggc ctgggccgcc ggggagaagg gggccttttg ccgccaggcc
     1801 aacgacaggg tgcgctgggc cttcggcgag gacagggccg cgtaggcgac cccggggaac
     1861 cgcgccacca tgcggggcac caggctgatg cccagccccg ccgccaccag ggcctgcacg
     1921 gtctcgatgc cgccgctctc gaagaccacc cggggttcca ggccctcctt acggtaaagc
     1981 tcgagcgtga tctgccggaa cccgtggccc gtcttcatca ggatgaaggg gtccggggcc
     2041 acctgcttga gcgccagggc cttcttcccc gccaagggat gcttgggcgg aagggccagc
     2101 agcagttcct cggtcaagac ggcctggtgg cccagggaag gcctcaggcc cgcctcgtcc
     2161 aaaatggcca gctcgatctc gccctgctcc aggccctggg ccaggtcggg cgaggattct
     2221 tccttcagct gcacctggat ggtcgggtgg gcctttttga aggccgtcaa aatgggggga
     2281 agcaggtggg cgcccgtggt gggcatgcag cccaaggcca ccttgcccag gcgcagcccc
     2341 gaaagcgccg cagcgtcctc ctgggcggcc tccatctggt ccaggatcgc gcgggcccgg
     2401 ggcaggaaag cctccccccg ggcggtcagg cgcgcgccct tgcggccccg ctcgaacagg
     2461 gccccgccca attccccctc caacttggcg acttggatgc tcaaggaagg ttgggacacg
     2521 tggcaggctt gggcggccgc cgtaaagcgc cccgtctggg ataccgccag gaaatagcgc
     2581 agttgatggg tctccatatc atagttataa actatgttta atatacaaac tatatctttt
     2641 acaaataccc attccggccc taggctcttc ccatcacccg cttgggagga cgttatggca
     2701 aagggaacgt tgtacaacaa ggtctgggac gcccacacgg tggacaagct gcccacgggc
     2761 cagacccagc ttttcatcgg gttgcacctc atccacgagg tcaccagccc ccaggccttc
     2821 gacatgatcg ccgagaaggg gctgaaagtg gcctacccgg cccgcacctt cgccacggtc
     2881 gaccacatcg tgcctaccca ggaccaggcc cggcccttcg ccgacggcga ggccgaggaa
     2941 atgatggggg ccatcgagaa gaacgtgcgc caattcgggg tgaagttctt cgacttcaag
     3001 tcgggcgacc agggcatcgt gcacatcgtg gggcccgaac agggg
//
LOCUS       DASTJJ010000480         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_555913, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000480 DASTJJ010000000
VERSION     DASTJJ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555913"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFR02_05905"
     CDS             <1..719
                     /locus_tag="VFR02_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9870013.1"
                     /translation="ITERKQVDDMKRDLISVVSHQLKTPVAEINGYVENLLEGLAGPL
                     SPKQREYLADMREIGMENYRLISDLLSLSKLERGIITVDLQPVPLDQLVELSIRDYTS
                     MIQRKGLDLVLDQAPGGLQVLADRDKTVETLRNIINNALKCTDKGGITIATGAENGYG
                     WIKVTDTGIGMDRSVLKKLFTKDRVLGAEASRSGAGLGLYIAKSFMELQKGAISVESQ
                     AGKGSSFCLKIPRTEKEPVS"
     gene            716..>1006
                     /locus_tag="VFR02_05910"
     CDS             716..>1006
                     /locus_tag="VFR02_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870014.1"
                     /translation="MSKGTILIVEDQQGFRRIYRDVLENDGYQVLEAEDGEAGWEAAR
                     DKKPSLILLDLGLPKIDGFEVLKRIRADEGTKAIPVIIFSVLGEQKDVNRGLE"
BASE COUNT          224 a          302 c          326 g          154 t
ORIGIN      
        1 acatcaccga gcgcaagcaa gtggacgaca tgaagcggga cctgatctcg gtggtgtcgc
       61 accagctcaa gacccccgtg gccgagatca acggctacgt ggagaacctg ctggaggggc
      121 tggccggacc cctgtccccc aaacagcggg agtacctggc cgacatgcgg gagatcggca
      181 tggagaacta ccggctgatc tcggacctcc tgagcctgtc caagttggaa cgcggcatca
      241 tcacggtcga cctccagccc gtccccctgg accagctggt ggaactgtcc atccgggatt
      301 acacctccat gatccagcgc aagggcctgg acctggtgct cgaccaggcc ccggggggcc
      361 tccaggtgct ggcggaccgg gacaagacgg ttgaaacgct gcgcaacatc atcaacaatg
      421 ccttgaaatg caccgataaa ggcggcatca ccatcgccac cggggccgag aacggctacg
      481 gatggatcaa ggtgaccgac acgggcatcg ggatggaccg atccgtgctc aagaagctct
      541 tcaccaagga ccgggtgctg ggcgcggaag cctcccggtc cggggcgggg ttgggcctct
      601 acatcgccaa gagcttcatg gaacttcaaa agggcgccat ttcggtggag tcccaggcag
      661 gcaagggcag ttcattctgc cttaaaatcc caaggacgga aaaggagccg gtgtcatgag
      721 caaagggacc atcttgatcg tggaggacca gcagggcttc cggcgcatct accgggacgt
      781 gttggagaac gacggctacc aggtcctgga agccgaggac ggcgaggccg gctgggaggc
      841 cgcccgcgac aaaaaaccca gcctcatcct gttggacctg gggctgccca agatcgacgg
      901 cttcgaggtg ctgaaacgca tccgggccga cgaggggacc aaggccattc cggtcatcat
      961 cttttccgta ctgggcgagc aaaaggacgt caatcggggc ctggaa
//
LOCUS       DASTJJ010000481         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_434500, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000481 DASTJJ010000000
VERSION     DASTJJ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434500"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFR02_05915"
     CDS             complement(<1..540)
                     /locus_tag="VFR02_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9870015.1"
                     /translation="MIEIEHLTKRFGPILAVTDLTLVVRPGEVLGFLGPNGAGKSTTM
                     KMVTGYLAPDAGTVRICGHDIVSDTLAAQKRLGYLPEGAPAYGEMTPREFLHFIAKVR
                     ELSGSDAKTAIQHAVQGAELQSVLDQPIETLSKGFRRRVGLAQAILHDPEVLVLDEPT
                     DGLDPNQKHAVRGLIRGMAK"
     gene            629..1003
                     /locus_tag="VFR02_05920"
     CDS             629..1003
                     /locus_tag="VFR02_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870016.1"
                     /translation="MVNLKILRRATAVGVMFELLLVVAAHYRPWLTRHFLLFGCMLIA
                     GVAGLLYARDLARGFGPGAMGGAATGALCGVVAYEASNLFGDRPDIYIPLGVLVLTLT
                     GAVGGLFGQLDAVFKAFIRSQK"
BASE COUNT          150 a          324 c          346 g          220 t
ORIGIN      
        1 cttggccatg ccgcggatca ggcctcgcac cgcgtgcttc tggttgggat cgagtccgtc
       61 ggtcggctcg tccaggacca ggacctcggg atcgtggaga atggcctggg ccagccccac
      121 ccggcgccgg aaccctttgg acaaggtctc gatgggctgg tcgagcacgc tttggagctc
      181 cgccccctgg acggcgtgct gaatggccgt cttggcgtcg gatccgctca attcccgcac
      241 cttggcgata aaatggagga attcgcgcgg ggtcatctcg ccgtaggccg gcgccccttc
      301 gggcaggtag cccagccttt tttgcgccgc gagcgtgtct gagacgatat cgtggccgca
      361 gatgcgcacc gtgccggcat cgggggccag atagcccgtc accatcttca tggtggtgga
      421 tttgcccgct ccgttgggcc ccaggaaacc caaaacctcc cccggcctta ccactaaagt
      481 aaggtcggtc accgccagga tgggtccgaa gcgcttggtg aggtgctcga tttcgatcat
      541 agtggcggga accgggtttt tctccgttta gagaaatcgc caggcagccg caagcggacc
      601 tctcgtcacg gtccttaagg agtgtcgcgt ggtcaatctc aagattttgc gccgcgccac
      661 tgccgttggc gtgatgttcg agcttcttct ggtggtggcg gcgcattacc ggccctggct
      721 cacgcgccat ttcctgttgt tcggctgcat gctgatcgcg ggcgtggcag gcctgctcta
      781 tgcccgcgac ctggcgcgcg gctttggccc gggcgccatg ggcggggcgg cgaccggggc
      841 cttgtgcggg gtggtggcct atgaagcctc gaacctgttc ggcgaccggc ccgacatcta
      901 tatccctttg ggggtgctgg tcctcaccct gaccggcgcg gtgggcgggc ttttcggcca
      961 gctcgacgcc gtgttcaagg ccttcatccg ctcacagaaa taattccggg atgaagtgtc
     1021 ggttggcgcg attcgcgacc
//
LOCUS       DASTJJ010000482         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_190844, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000482 DASTJJ010000000
VERSION     DASTJJ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190844"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..>1586)
                     /locus_tag="VFR02_05925"
     CDS             complement(305..>1586)
                     /locus_tag="VFR02_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9870017.1"
                     /translation="LCPPGPLWADVQILTGVPSTYMFPAEGFTPLDLHRGTQTLLSLM
                     LPGTTFDDPQGMACALLKRDHDPQAPLNDVVVTVLGVNSGAGEVLYNVGLKDLKRYGS
                     PGGGVGQFRGPTGAALNSDGSAAIADTGNDRVVLLHDDGLRLRWVGTVPGTFHAPQGV
                     AYDTAGDLFIADTGADRVLVRPPHGAVRVLPLQGLDHPTALAVIDSKADWTFYKTGPL
                     ADRLAVIDRMGARLSTYTLAGAPLAQFTSDQAPDGPMRLWGCAFDYYGNLVATDTAAG
                     CLRKFGRNLEYLATFGSPGVGDYQFDHPRGICLNTQFGQMFVANRQSADYYWVGADAR
                     DLAFQRTPQGFRFPFFLTERALVTAKVLDGQGAPVKDLGAGMDLEEGAQELDWDPDPS
                     LPAGTYTLRLTVMATYSTREREAKRLELSVPYSK"
BASE COUNT          262 a          506 c          547 g          271 t
ORIGIN      
        1 cctcgatgtg gtaggtcacc cccccggcgg tctcattttg gcggctggta tggagaattt
       61 taggtacggc catggccccc cctcgatgcg gattttcgac ggcttccagt ttaccccctc
      121 cccgggggac gcgggtttcg aacttggaaa ttattcggga tcctgcactc cgcaaagcaa
      181 aggccattgg aacattgata aaaccgataa agcccgattt ttattctcaa acatcggatt
      241 tcatcgatcc ttatcattgt tccaaggctt ctttgcctgc tttgtaaaag acatggttgc
      301 gaaattattt actgtaaggc acggacaatt cgagccgctt ggcttcccgt tcccgggtcg
      361 aataggtggc catgacggtc aagcgcaggg tgtaggtgcc cgcgggcagc gaggggtctg
      421 gatcccagtc cagttcctgg gccccctcct ccaggtccat gcccgctccc aggtccttca
      481 cgggggcgcc ttggccgtcg agcaccttgg cggtgaccag ggcccgctcg gtgaggaaaa
      541 aggggaaccg gaacccctgg ggcgtgcgct ggaaggccag gtcccgggcg tccgcgccca
      601 cccagtagta gtccgcgctc tgccggttgg ccacgaacat ctggccgaac tgggtgttca
      661 ggcagatgcc ccggggatgg tcgaactgat aatcgccgac gcccggggaa ccgaaggtgg
      721 ccaggtattc caggttgcgg ccgaacttgc gcagacagcc ggcggcggtg tccgtggcca
      781 ccaggttgcc gtaataatcg aaggcgcaac cccacaaccg catggggccg tcgggggcct
      841 ggtcggaggt gaattgggcc agcggcgcgc ccgccagggt gtaggtggaa agccgggcgc
      901 ccatccggtc gatcaccgcc aaccggtcgg ccagcgggcc cgtcttgtag aaggtccagt
      961 cggccttcga gtcgatgacc gccagggcgg tgggatggtc gagcccctgg aggggcagga
     1021 cccggaccgc gccgtggggg ggccgcacca gcacgcggtc ggcgcccgtg tcggcgatga
     1081 aaaggtcgcc cgccgtgtcg taggccacgc cctggggcgc gtggaaggtc ccgggcacgg
     1141 tgcccaccca gcgcaagcgc aggccgtcgt cgtgcagcag caccacccgg tcgttgcccg
     1201 tgtcggcgat ggccgccgaa ccgtcgctgt tcagcgccgc ccccgtgggg ccgcggaact
     1261 ggcccacgcc cccgcccggg gaaccgtatc gcttcaggtc cttgagcccc acgttgtaga
     1321 gcacctcgcc ggcccccgag ttgaccccca gcacggtcac caccacgtcg ttcaggggcg
     1381 cctgggggtc gtggtcccgc ttcagcagcg cgcaggccat tccctggggg tcgtcgaagg
     1441 tggtgccggg cagcatgagc gacaacaggg tctgggtgcc ccggtgcagg tcgagcggcg
     1501 tgaagccctc cgcggggaac atgtaggtgg aggggacgcc ggtcaggatc tgcacgtcgg
     1561 cccacaaggg gccgggcggg cacagg
//
LOCUS       DASTJJ010000483         1436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1779983, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000483 DASTJJ010000000
VERSION     DASTJJ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779983"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /gene="trxA"
                     /locus_tag="VFR02_05930"
     CDS             complement(<1..263)
                     /gene="trxA"
                     /locus_tag="VFR02_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9870018.1"
                     /translation="MEAKNLFEATDGTFEKEVLQTKGPVLVDFWAEWCGPCKMLGPTL
                     HTISNEYAGKVKVVKMDVDSNPETPGKYHIHGIPTVILFKDGQP"
     gene            complement(280..465)
                     /locus_tag="VFR02_05935"
     CDS             complement(280..465)
                     /locus_tag="VFR02_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870019.1"
                     /translation="MNLLNPENQTQEAAEERALRELKDLVRSTDRRLDQLAVQTGEKE
                     PPAFFAAYEEAVSIVLN"
     gene            600..899
                     /locus_tag="VFR02_05940"
     CDS             600..899
                     /locus_tag="VFR02_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009199556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9870020.1"
                     /translation="MRKTHSADLQKTPLPKVFQALSDPARLGILRTLLEKGEAPCSEC
                     DLPVSKSTLSHHYKVLRQVGLITTRSEGTRQVNCLDRWAIERRFPGLLEWIDRME"
     gene            972..>1436
                     /locus_tag="VFR02_05945"
     CDS             972..>1436
                     /locus_tag="VFR02_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9870021.1"
                     /translation="MNLKFPAFLLSLLALAGSARAQSDPSDFKINVVEKVLQNGMTVL
                     VVERPGVPVVSFAYLQPVGAQDAPKGKTGLPHMLEHMMFKGTQTIGTKSYAKEKPILE
                     AMDKVAEDENAEKAKPDPDPDKLKALAAKMRELQQAEHQVDVNNELSTLYTLH"
BASE COUNT          284 a          474 c          401 g          277 t
ORIGIN      
        1 ggctgcccgt ccttgaacag gatgaccgtg gggatgccgt ggatgtggta cttgccgggc
       61 gtctccgggt tgctgtccac gtccatcttc acgaccttca ccttgcccgc gtactcgttg
      121 gagatggtgt gcagggtcgg ccccagcatc ttacagggcc cgcaccactc ggcccaaaaa
      181 tccaccagca cgggcccttt ggtctggagc acttccttct caaaggtgcc gtcggtggcc
      241 tcaaacaggt tcttcgcttc catggaatgc ctcctttagt cagttcaaaa cgatggacac
      301 agcctcttca taggcggcga aaaaggccgg gggttctttt tcccccgtct ggacggccag
      361 ttggtcgagc cgccggtcgg tcgaacggac caggtccttc agctcccgca gggccctttc
      421 ctcggccgct tcttgggttt gattttccgg gttcagcaga ttcatggcac cctcctcccg
      481 ccctgtccaa cgccttcatc cttgccgttg aatggttcga tgatattcga acaataggac
      541 tggcccccgg cgccgtcaag tcctatactc caggaaaggc tgaaaccaag gggaaaacca
      601 tgcgaaaaac ccattccgcc gacctgcaaa agaccccgct cccgaaggtc ttccaggccc
      661 tcagcgaccc cgcccggctg gggatcttaa ggaccctcct ggaaaagggg gaggccccct
      721 gcagcgagtg cgacctgccg gtgagcaagt ccaccctttc ccaccactat aaggtcctgc
      781 gccaggtcgg gctcatcacg acccgcagcg aggggacccg acaggtcaat tgtctggacc
      841 ggtgggccat tgagcgccgt ttccccggac tattggagtg gatcgatcga atggaatgac
      901 ccggcctttt tcctgtcctt ttgcccgacc cctggttaaa ctttccctcg tctaaaaaac
      961 cgggagtcac catgaacctc aaatttccgg cgttcctctt gtccttgctg gccctggccg
     1021 gttcggcccg ggcccaatcg gatccctcgg acttcaagat caacgtggtg gagaaggtcc
     1081 ttcagaacgg catgaccgtc ctggtggtgg aacgcccggg cgtgccggtg gtctccttcg
     1141 cctacctgca gcccgtgggg gcccaggacg cgcccaaggg caagaccggc ctgccccaca
     1201 tgctggaaca catgatgttc aagggcaccc agaccatcgg gaccaaatcc tacgccaagg
     1261 aaaaacccat cctggaggcg atggacaagg tggcggagga tgagaacgcc gaaaaggcca
     1321 agcccgaccc cgatccggac aagctcaagg ccctggcggc caagatgcgg gaactgcagc
     1381 aggccgagca ccaggtggac gtcaacaacg agctctccac cctctacacc ctccac
//
LOCUS       DASTJJ010000484         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1536056, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000484 DASTJJ010000000
VERSION     DASTJJ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536056"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFR02_05950"
     CDS             <1..293
                     /locus_tag="VFR02_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9870022.1"
                     /translation="MELRRGFSVGKGERLFAVEDVITTGGSVKEAIGVVRGLGADVVG
                     VGSLIDRSGGADKAFDVPMKALVTLAVESWEEKDCPLCRQGLPVEKPGSRAK"
     gene            290..559
                     /locus_tag="VFR02_05955"
     CDS             290..559
                     /locus_tag="VFR02_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870023.1"
                     /translation="MRPGEKEAPAVRPSPAWGPYGRSLLAAWAFVGASMVVAFFVCGR
                     VWDDVTSGVMEFLVVVLGGGFTLMTAVDYFYEKYNGPGARGDRKP"
     gene            556..1512
                     /locus_tag="VFR02_05960"
     CDS             556..1512
                     /locus_tag="VFR02_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870024.1"
                     /translation="MKSFAAKACAFLLVFCTASGSFPVQWLYAQTQGDAASADEAQEL
                     RHLAQTGYLGAKTLGPEGSSLTQDQVLDALLAIQEGLKAVDLKGLAPGDPRYQLEDLQ
                     DLLQMVEDHAGPLRDRKVSAWLYENRLKKMIAALTPAAPAPTGTPTAVPPSPTPTPVP
                     GPGWDDYNALQGKLKDLNQRLADLQAKYDQKLQAEDSASSLLQSHDQERQDELKLVKN
                     LMDTYESNLQKVNQRMDQVAEKADRKSLSDTELEQELSIMHKDLRDNTQDLTVLKQQV
                     AQLDAPQQAPKDPLDDFLSSKWLAGGALVVGLAALTVALTHK"
BASE COUNT          292 a          496 c          578 g          270 t
ORIGIN      
        1 ccatggagct gcgccggggt ttttccgtgg gcaagggcga gcgccttttc gccgtggagg
       61 acgtcattac cacggggggg tcggtgaagg aggccatcgg ggtggtgcgg ggattggggg
      121 ccgacgtggt gggcgtgggg agcctgatcg accgcagcgg cggcgcggac aaggccttcg
      181 acgtgcccat gaaggccctg gtgaccctgg cggtggaaag ctgggaagaa aaggactgcc
      241 ccctctgccg gcaagggctg ccggtggaaa aacccggatc gagggccaag tgaggcccgg
      301 ggaaaaagag gccccggccg tccggccgtc gcccgcctgg gggccctatg ggcgttccct
      361 gttggccgcc tgggccttcg tgggggcttc gatggtcgtg gcctttttcg tgtgcgggcg
      421 ggtctgggac gacgtgactt ccggggtgat ggaattcctg gtggtggtat tgggcggcgg
      481 gtttacactg atgacggcgg tcgactactt ctacgaaaaa tacaacgggc ccggggcccg
      541 gggggaccgg aaaccatgaa atccttcgcg gccaaggcct gcgccttcct gttggtcttt
      601 tgcacggcgt cgggatcctt cccggtccaa tggctctatg cccaaaccca gggcgacgcg
      661 gcgtccgccg acgaagcgca ggaactccgg cacctggccc aaaccggcta tttgggggcc
      721 aagaccctgg gcccggaggg gtcgtccctg acccaggacc aggtgctgga cgcgctcctg
      781 gccatccagg aagggttgaa ggcggtggac ttgaagggcc tggcgccggg cgatccccgg
      841 taccagttgg aggacctgca ggacctgctt cagatggtgg aggaccacgc gggccccttg
      901 cgggaccgca aggtgtcggc ctggctttac gagaaccggt tgaagaagat gatcgcggcc
      961 ttgacgcccg ccgcgcccgc ccccacgggc accccgaccg cggtcccgcc ttcccccacg
     1021 cccaccccgg tgcccgggcc cggctgggac gactacaacg ccctgcaggg caagttgaag
     1081 gacctgaacc aacgtttggc ggacctgcag gccaagtacg accagaagct ccaggccgag
     1141 gattcggcgt ccagcctgct ccagtcccac gaccaagagc ggcaggatga gctgaagctg
     1201 gtgaagaacc tgatggacac ctatgagtcc aacctgcaga aggtcaacca gcggatggac
     1261 caggtggccg aaaaggccga ccggaagtcc ttgagcgaca ccgaattgga gcaggaactc
     1321 agcatcatgc acaaggacct gcgggacaac acccaggacc tcacggtgct gaagcagcag
     1381 gtcgcccagt tggacgcgcc ccaacaagcg cccaaggacc cgttggacga tttcctgtcc
     1441 tccaaatggt tggccggcgg cgccctggtg gtggggttgg cggccttgac ggtggctttg
     1501 acccacaaat aaaggaggca tcatgttcat cgggcatttc gcggtggctt tggcgggtaa
     1561 aaaggcggct cccaaggcgt ccctggggac gttgttcctg gcggcccagt tcctggacgt
     1621 cctttggccc atcttc
//
LOCUS       DASTJJ010000485         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1413522, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000485 DASTJJ010000000
VERSION     DASTJJ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413522"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1072)
                     /locus_tag="VFR02_05965"
     CDS             complement(<1..>1072)
                     /locus_tag="VFR02_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870025.1"
                     /translation="MLAALACLLLFELLTRWVRSPAAWLLSLFWVVGAVSYPGALSAK
                     TGLYQLAAGLLVALVLALAQARVEAAGFLFGLGLACHWMTFLALAPGLLLFAWDRRPW
                     EFKRLATSLAFMALGLSLYLYLPLRAAQGPLLDWGHPVSWRDFWFDFTRAQYGGGEGA
                     SWGSALAQGWALLKDDLWEWPGFLLAAFAGVWASWRRDRKEARGLAAAWLFWSAALGF
                     FLHLSADRFYLLKGYALAGEALVLVFVGFALRELPPVSRRFWVPALALFWAVLAVLRF
                     ATARQTDYTYAYDYTLNSFQEIPKGGLYFARGDSLVFPAWYFQWVEGRRPDLAVVGVD
                     GLPMGWVRRTLARTHPDLHVPES"
BASE COUNT          242 a          377 c          345 g          108 t
ORIGIN      
        1 gctctcgggc acgtgcaggt cggggtgggt tcgggccagc gtgcgccgca cccatcccat
       61 gggcaatccg tccaccccca ccaccgccag gtcgggcctc ctgccttcca cccactggaa
      121 ataccaggcc ggaaagacca ggctgtcgcc ccgggcgaaa tagaggccgc ccttggggat
      181 ctcctggaag ctgttgagcg tgtagtcgta ggcgtaggtg tagtcggtct gccgggccgt
      241 ggcgaagcgc aagaccgcca acaccgccca gaaaagcgcc aacgccggca cccaaaaacg
      301 ccgggagacc ggcgggagct ccctcaaggc gaaaccgacg aagaccagca ccagggcctc
      361 ccccgccaag gcgtagccct tcaatagata gaaacggtcg gcggaaagat gcaggaaaaa
      421 acccaaagcc gcggaccaaa agagccaggc ggcggccaag ccccgcgctt ccttccggtc
      481 ccgccgccag gaggcccaga ccccggcgaa agccgccaaa aggaacccgg gccattccca
      541 aagatcgtcc ttcaacaacg cccagccttg cgccagggcg cttccccagc ttgcgccttc
      601 cccacccccg tattgggccc gggtgaagtc gaaccaaaaa tccctccaag agaccggatg
      661 gccccaatcc agcaggggcc cttgcgcggc ccgcaggggt agatagaggt aaagcgagag
      721 gcccagggcc atgaaggcca gggatgtggc cagccgtttg aactcccagg gccgccggtc
      781 ccaggcgaaa agaagcaggc cgggcgccag ggccaaaaag gtcatccagt ggcaagccag
      841 gcccaagccg aagaggaacc cggccgcctc gacccgggcc tgcgccaggg ccaggaccaa
      901 ggccaccaac agccccgccg ccagttggta caggcccgtc ttggcgctga gggccccggg
      961 gtaggagacg gcccccacca cccagaagag ggacaggagc caggccgcgg gggaacggac
     1021 ccagcgggtg agcagctcaa aaagcagcag gcaggccagg gcggccagca tc
//
LOCUS       DASTJJ010000486         1446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_314043, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000486 DASTJJ010000000
VERSION     DASTJJ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314043"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFR02_05970"
     CDS             complement(<1..1193)
                     /locus_tag="VFR02_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9870026.1"
                     /translation="MATATTTAAPAAPAKAGGLSREGVMLILTAGIASLLEIVDTSIV
                     NVAIPTMMGNLGATLDDIGWVVTGYIISNAVVLPISGWLGLRFGRRSYYTTCIVLFVL
                     ASVACGLAPNLETLIAFRVLQGAAGGALLPTSQALIQEAFPGRSGFGSALYGMVVIIG
                     PTIGPPLGGFLTDHFGWRMIFNINVPFGILATFMSLAYVKDHAVEAEHAAAHHKARTA
                     PVDLVGLALLIVGIGCLQFVLERGQADDWFASAHILVMALVAGVSLPGFIWWELTTEH
                     PILDLRLYKNPALLNGTLLMGAVGFMLYSLIFFVPIFASNILGLDSGQTGNLFVPAAL
                     LAGFMMPVIGSQFSKYDPRAFMFLGLLAIEGCLLFLAHFTPQSTYQDLVDSQLIRGLG
                     MPFLF"
BASE COUNT          301 a          434 c          484 g          227 t
ORIGIN      
        1 atgaagagga agggcatgcc caggccccgg atgagctggg aatcgaccag gtcctggtag
       61 gtggactggg gcgtgaagtg ggccaggaac aacaggcagc cttcgatggc caacaggccc
      121 aggaacatga aggccctcgg gtcgtacttg gagaactggc ttccgatgac cggcatcatg
      181 aagccggcca ggagcgccgc cggcacgaag aggttccccg tctggcccga gtccagcccc
      241 aggatgttgc tggcgaaaat gggcacgaaa aagatcaggc tgtagagcat gaagcccacc
      301 gcgcccatga ggagcgtgcc gttcaaaagc gccgggttct tgtagagccg gaggtccagg
      361 atggggtgct cggtggtcag ttcccaccag atgaagccgg gcagggagac gcccgccacc
      421 agggccatga ccaggatgtg ggcgctggcg aaccagtcgt cggcctggcc ccgttccagc
      481 acgaactgca ggcaaccgat gcccacgatc aagagcgcca gccccaccag gtccacgggg
      541 gccgtgcggg ccttgtggtg ggcggcggcg tgttcggctt ccaccgcgtg gtccttcaca
      601 taggccaggg acatgaaggt ggccaggatg ccgaagggca cgttgatgtt gaagatcatg
      661 cgccagccga agtggtcggt caggaacccc ccgagcgggg ggccgatggt gggcccgatg
      721 atgaccacca tgccatagag ggcgctgccg aaaccggacc ggcccgggaa ggcctcctgg
      781 atgagcgcct gggacgtggg caaaagcgcg ccgcccgccg cgccctgcag cacccggaag
      841 gcgatcaggg tctccaggtt gggcgccagg ccgcaggcca ccgaggccag cacgaagagc
      901 acgatgcagg tcgtgtagta gctgcggcgg ccgaaccgaa gccccagcca gcccgagatg
      961 ggcagcacca cggcgttgga gatgatgtag ccggtcacca cccagccgat gtcgtccagg
     1021 gtcgccccca ggttgcccat catggtgggg atggccacgt tcacgatgga cgtgtccacg
     1081 atctccagca aggacgcgat gcccgccgtg aggatgagca tcaccccctc gcgggaaagc
     1141 cccccggcct tcgcgggggc ggccggggcc gccgtggtgg tcgctgtcgc cataaaaagc
     1201 tccttgagaa cttctaacca agacctgttt caccacacac acccaagggc ggccccgttc
     1261 caaaattcaa ggatggggca aacaccaagt tcaccaagaa aggcaataga atttattcgg
     1321 attgtctgtc tttgcagtcc ttggtgccct tggtgccttg gtggtgaaaa ttgctttcat
     1381 tttgatcttt gttagaaact tttaactcga ctcaacaaag gggccggccc ggggtcaatg
     1441 cacctg
//
LOCUS       DASTJJ010000487         1338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_679897, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000487 DASTJJ010000000
VERSION     DASTJJ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679897"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFR02_05975"
     CDS             <1..194
                     /locus_tag="VFR02_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870027.1"
                     /translation="YFRIFDLEMVAKPRLYRYSPAAGDRPRPRPCVPKLGTGASVDKK
                     KEPDKRRSLRWIFQGKNEK"
     gene            313..627
                     /locus_tag="VFR02_05980"
     CDS             313..627
                     /locus_tag="VFR02_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9870028.1"
                     /translation="MPYVISEKCLGEVYAQCVDVCPVNCIHPGDYKGEKFMVIDPEEC
                     TECTSCYSACPIGAIVEGPDKDPEYGKINAELAPSFKNNPAVATRPANDPPKKPGNKL
                     VN"
     gene            complement(726..>1338)
                     /locus_tag="VFR02_05985"
     CDS             complement(726..>1338)
                     /locus_tag="VFR02_05985"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET9870029.1"
                     /translation="IEGPGHVPMQLIKENMDKQLAECHEAPFYTLGPLTTDIAPGYDH
                     ITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKNDVRDGVIAYKIAAHAADLAKGHPAA
                     QRWDNALSKARFEFRWEDQFNLALDPERAREFHDETLPADGAKVAHFCSMCGPKFCSM
                     KITQDVRDYANSHGLTEEKALEAGMKQMSDNFVGTGSEIYQKV"
BASE COUNT          259 a          410 c          404 g          265 t
ORIGIN      
        1 actactttcg catttttgat ctggaaatgg tagccaaacc ccggctttac cgttattccc
       61 cggcggccgg cgaccgaccg cgtccacgcc cctgcgtccc aaaattagga acgggagcgt
      121 ccgtcgacaa gaagaaggaa ccggacaagc ggcggtcctt gcggtggatt tttcagggga
      181 aaaacgaaaa atgagttgcg caataaaccc cggctgccta aactaacgtt gttttccagg
      241 ttttaggcga aaaaaccatt tccgccccgg ccttggcgcc ggacggaagc cactttctat
      301 aaggaggccg tcatgccgta cgtcatttct gaaaagtgct tgggtgaggt ttacgcgcaa
      361 tgcgtggacg tctgcccggt gaattgcatc catcccgggg actacaaggg cgagaagttc
      421 atggtcatcg accccgagga atgcacggaa tgcaccagct gctactcggc ctgccccatc
      481 ggcgccatcg tggaaggtcc ggacaaggat cccgagtacg gcaagatcaa cgccgaactg
      541 gccccgtcct tcaagaacaa cccggctgtg gcgacccgcc cggccaacga ccccccgaag
      601 aagcccggca acaagctggt caactaaggc ccagcctccg gtttttcgcc tagcctgata
      661 gaagacaaaa agcccttccc ccttgagggg gaagggcttt ttatttgcca gggcccgcgg
      721 ccgttttaga ccttctggta gatctccgac cccgtgccca cgaaattatc ggacatctgt
      781 ttcatgccgg cttccaaggc cttctcttcg gtcaacccgt gggagttggc gtagtcccgc
      841 acgtcctggg tgatcttcat gctgcagaat ttcggcccgc acatggagca gaaatgggcc
      901 accttggcgc cgtccgcggg cagggtctcg tcgtggaact cgcgggcccg ctccgggtcc
      961 agcgccaggt tgaactggtc ctcccagcgg aactcgaacc gggccttgga cagcgcgttg
     1021 tcccaacgct gggccgccgg gtggcccttg gccaggtccg ccgcatgggc cgcgatctta
     1081 taggcgatga ccccgtcgcg cacgtcgttc ttgttgggca gccccaggtg ttccttgggc
     1141 gtgacgtagc agagcatggc ggtgccgaac cagccgatca tggccgcgcc gatggcgctg
     1201 gtgatgtggt cgtaaccggg ggcgatatcg gtggtcaggg ggcccaaggt gtagaaaggc
     1261 gcctcatggc actcggcgag ctgcttgtcc atgttctcct tgatgagctg catgggcacg
     1321 tggcccggac cctcgatc
//
LOCUS       DASTJJ010000488         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_192291, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000488 DASTJJ010000000
VERSION     DASTJJ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192291"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="lptC"
                     /locus_tag="VFR02_05990"
     CDS             <1..511
                     /gene="lptC"
                     /locus_tag="VFR02_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET9870030.1"
                     /translation="AGKKAGLGEPGQGPASPDATIDKFHLISTALGMKHWELYATQAK
                     LYQNLKEADTQDIYAEYYKNNKMVSNLTADKAVINTETNATVVEGHVELITENGSKLQ
                     TEKLAWDPDTDLLHTNEPVHVYKGSDDITAVGLTADTELDHIQFTKDVHTQVRDTHEI
                     ADFNRPKPF"
     gene            474..1415
                     /locus_tag="VFR02_05995"
     CDS             474..1415
                     /locus_tag="VFR02_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET9870031.1"
                     /translation="MRSPTSTDPNPFNARALAVLGWLTAGAAWAGPSAPTAPLAPGAT
                     PAPAAQALDTGKRGEVSSRKPVDISSDQLSFDRLGGQTLFTGDVKVLHDQLTLTSDQL
                     ETTEGNRQASATGNVRVDDLKSGLHLTCGNLDYADLMDRITAHDHPQLTTVDGNGVPV
                     TLRARQLELDSDVKRVTAHQDVEIFRDDSRSEAQLATFDSKTDQLVLEENPRIFMPQG
                     VIGGRRITTSLSGDHRIVVEGMAEAEFYVQPKPVPTKPPGAGSSTPLGITPGAAPTPG
                     SASPAVRPAAPPTPTAPGGIRPPVPVPPPGAPLPGAS"
     gene            1494..>1791
                     /locus_tag="VFR02_06000"
     CDS             1494..>1791
                     /locus_tag="VFR02_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9870032.1"
                     /translation="MRILRTRELVKTYNKKKVVDHISIEVKQGEVVGLLGPNGAGKTT
                     TFYMTTGFIPPDRGQVYFEEDGKLQDITHTPMYKRARLGIGYLPQEASIFRKLTV"
BASE COUNT          354 a          676 c          528 g          233 t
ORIGIN      
        1 cgcgggcaag aaggcgggcc tgggcgagcc cggccagggg ccggcctcgc cggacgccac
       61 catcgacaag ttccacctca tctccaccgc cctcgggatg aagcactggg aactctacgc
      121 cacccaggcc aagctctacc agaacctcaa ggaagcggac acccaggaca tctacgccga
      181 gtactacaag aacaacaaga tggtctccaa cctgaccgcg gacaaggccg tcatcaacac
      241 cgagaccaac gccaccgtgg tggagggcca cgtggagctc atcacggaga acgggtccaa
      301 attgcagacc gagaagctgg cctgggaccc ggacaccgac ctgctccaca ccaacgagcc
      361 ggtccacgtg tacaagggct ccgacgacat cacggccgtc ggcctcacgg ccgacaccga
      421 gctcgaccac atccagttca ccaaggacgt ccatacccag gtgagggaca cccatgagat
      481 cgccgacttc aaccgaccca aaccctttta acgcccgcgc cctggccgtc ctggggtggc
      541 tgacggcggg cgcggcctgg gccggcccct cggcccccac ggccccgctg gcgccggggg
      601 ccacccccgc cccggccgcc caggccctgg acacgggcaa acgcggcgag gtctcgtccc
      661 gcaagcccgt ggatatttcc tccgatcagc tgagctttga ccggctgggg ggccagaccc
      721 tcttcaccgg cgacgtgaag gtgctgcatg accagttgac cctcaccagc gaccaattgg
      781 agaccaccga ggggaaccga caggcttcgg ccaccgggaa cgtccgggtg gacgacctga
      841 agtccggcct gcacctgacc tgcgggaacc tggactatgc cgacctcatg gaccgcatca
      901 cggcccatga ccacccccaa ctgaccacgg tggacggcaa cggcgtcccg gtgaccctgc
      961 gggcccgcca gttggaactg gactccgacg tcaagcgcgt gaccgcccac caggacgtgg
     1021 aaatattccg ggacgacagc cggtccgagg cccaattggc caccttcgat tccaagaccg
     1081 accaactggt cctggaggaa aatccccgca tcttcatgcc ccagggggtc atcgggggac
     1141 ggcgcatcac cacgtccttg tccggcgacc accgcatcgt ggtggagggg atggcggagg
     1201 ccgaattcta cgtccagccc aagcccgtgc ccaccaaacc gcccggcgcg ggaagttcca
     1261 ctcccttggg catcaccccc ggcgccgcgc ccaccccggg atccgcgtcg ccggccgtcc
     1321 ggcccgccgc gccccctacc cccacggccc ccggcgggat ccggcccccc gttcccgtcc
     1381 ctccgcccgg agcgccgctc cccggggcgt cctgaccggc aaaaagtcgt ttgaaggggc
     1441 ccggccctcc gctatgctgg gtcggcgtcc caacccctgg caacgaggcc ccgatgcgga
     1501 tcctgcgcac ccgtgagctg gtcaaaacct acaacaagaa aaaggtcgtc gaccacatct
     1561 ccatcgaggt caaacaaggg gaggtggtgg gattgctggg ccccaacggc gccggcaaga
     1621 cgaccacctt ctacatgacc accggcttca tcccgcccga tcggggccag gtctatttcg
     1681 aggaggacgg aaaactccag gacatcaccc atacgcccat gtacaagcgg gcgcgcctgg
     1741 ggatcggcta cctgccccag gaagcctcca tcttccgcaa gctgaccgtc g
//
LOCUS       DASTJJ010000489         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1659751, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000489 DASTJJ010000000
VERSION     DASTJJ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659751"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFR02_06005"
     CDS             complement(<1..311)
                     /locus_tag="VFR02_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET9870033.1"
                     /translation="MAQLKKIFIAVTGASGTIYARRFFQHLPYDDWEVHACVSSSARI
                     VASHEGGLNLPPHVREWDEKDLTAPVASGSNRFEAMVVVPCSTATLGKLAHGIADNLI
                     TR"
     gene            complement(299..814)
                     /locus_tag="VFR02_06010"
     CDS             complement(299..814)
                     /locus_tag="VFR02_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870034.1"
                     /translation="MEPIMSGPEGKEFHSRVSWWAIPIYCLFYVFPLVFLMLGVTHHG
                     FRFFILSFISGSALVWMLLYIERPVTGISCSGTSVVFTDPLFLKTKMSWSEIRKVQII
                     YEGDWLGIVRVFGPNPPFRIPFKILTRTFPKKEMMEMLEWLKNNASQAEIKNVDLLKK
                     DVSRDSYRWPN"
     gene            complement(819..1499)
                     /gene="ubiE"
                     /locus_tag="VFR02_06015"
     CDS             complement(819..1499)
                     /gene="ubiE"
                     /locus_tag="VFR02_06015"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET9870035.1"
                     /translation="MSQAIQQMFSRIAPTYDQANHALSFNQDIEWRKDSVQRAAGDGF
                     SPRQVLDLCAGTGDFALAAKAQWPGAQVTLADFSKPMLALAKGKGGGFNYLQADALKL
                     PFQDFSFDALLCGFGVRNLDSAEAGVREMARVLKPGGKAVVLEFFKPTGFLSKLAYGF
                     YVSTILPLRGGAISKDKAAYDYLQKSAKGFLALNEFKALLEKNGFKDVQVQAYTMGVA
                     ACLVAVRK"
     gene            complement(1608..1955)
                     /locus_tag="VFR02_06020"
     CDS             complement(1608..1955)
                     /locus_tag="VFR02_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9870036.1"
                     /translation="MAFTKEQVLKELKSDGVVILNVLPAGDYAKLHIKHSRNLPWGDG
                     QEAFARGAEAFGRDKFFITYCAGPGCSAGPEAARFLKEKGFRAEDYPGGMEEWHENGL
                     PVEGSLASASLSV"
     gene            complement(2090..3538)
                     /locus_tag="VFR02_06025"
     CDS             complement(2090..3538)
                     /locus_tag="VFR02_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HET9870037.1"
                     /translation="MAYESLSAFVAFLEKKGELARIPEELDPVLEIPALVDRVSKSPG
                     GGKALLFERPKGSAYPLLINAFGSKQRMAWALGVEDLEQHAAAIRDMLDLKPPAGLVE
                     KLKFLPKLAEIAKFPPVEASKGACQEVVEKDPDLLSLPVMQCWPQDGGRFITLPLVYT
                     QDPETGVRNIGMYRMQVHDSKTTGMHWQVHHGGAAHHRKAEKMGKRLEVAVALGGDPA
                     LTYAATAPLPEGVDELIFAGFLRKKPVELVKCKTVDLWVHADADFVLEGYVEPGERRR
                     EGPFGDHTGYYSLADDYPVFHVTCVTRRKAPVYPTTLVGKPPMEDGWLGKATERLFLP
                     LLQKLLPEITDLDMPVAGIFHNLAIVSIDKQYPQQARKVMYALWGLGQMMLNKVLVVV
                     DKDTDVHDYNQVLWRVGNCMDAKRDTVIVEGPLDVLDHASPQWGWGGKLGIDATRKWK
                     EEGFGREWPDELTMDPKVDEKVFPILKKHGLL"
BASE COUNT          704 a         1144 c         1125 g          697 t
ORIGIN      
        1 cgggtgatga ggttgtccgc gatgccgtgg gcgagcttgc ccagggtggc cgtcgagcag
       61 ggcaccacca ccatggcctc gaaacgattg gacccgctgg ccacgggggc cgtcaggtcc
      121 ttctcgtccc attcccgcac gtgcggcggc aggttcaatc ccccctcgtg cgaggccacg
      181 atgcgggcgg aggaggagac gcaggcgtgc acttcccaat cgtcataggg caggtgctgg
      241 aagaaccgcc gggcgtagat cgtgcccgag gcgccggtca cggcgatgaa tatctttttc
      301 aattgggcca ccggtaagaa tccctcgaaa cgtccttttt caacaggtca acgttcttta
      361 tttcggcttg ggacgcattg tttttcaacc actccagcat ttccatcatt tctttcttgg
      421 gaaaagtcct cgtcaatatt ttaaacggga tccgaaaggg gggattcggg ccgaaaaccc
      481 gcacgatccc cagccaatcc ccctcataaa tgatctggac ctttctgatc tcgctccatg
      541 acattttcgt cttcaaaaac aagggatcgg taaaaaccac acttgtccca gaacaagaga
      601 tcccagtcac cggcctttct atgtaaagca acatccaaac gagcgccgat cccgaaatga
      661 aggacaggat aaaaaaccga aacccgtggt gcgtcacccc caacatcaaa aaaaccaacg
      721 ggaaaacata gaacagacag taaatgggga tcgcccacca agagacccgg ctgtggaatt
      781 ctttcccttc gggcccactc atgatcggtt ccacttcatt acttcctcac ggccaccaag
      841 caggcggcca cgcccatcgt gtaggcctgg acctggacat ccttgaagcc gttcttctca
      901 agcaaagcct tgaactcatt caaggccagg aatcccttgg cggacttctg caggtagtcg
      961 taggcggcct tgtccttgga gatggcgccg ccccgcaggg gtaggatcgt ggagacgtaa
     1021 aagccgtagg ccagtttgga caggaagccc gtgggcttga agaactcgag gaccacggcc
     1081 ttgccgccgg gcttgagcac ccgggccatc tcccgcacgc ccgcctcggc tgagtccagg
     1141 ttccgaacgc cgaagccgca aagcagggcg tcaaaggaga aatcctggaa aggaagcttc
     1201 agggcgtcgg cctgcaggta gttgaaaccg ccgcccttgc ccttggccag ggccagcatg
     1261 ggcttggaaa agtcggccag cgtgacctgg gcgccgggcc attgggcctt ggccgccagg
     1321 gcgaaatcgc ccgtgcccgc gcaaaggtcc agcacctgcc ggggcgaaaa gccgtccccc
     1381 gccgcccgtt ggaccgaatc cttgcgccac tcgatgtctt gattgaagga cagggcgtgg
     1441 ttggcttggt cgtaggtggg ggcgattcgg gagaacatct gctggatggc ttggctcatg
     1501 ggtttcctta gggttgggat ggaagccgag cacttttata accaatttcc cggcaaataa
     1561 aaagcccgct tcccccgggg gaaacgggcc tgaaaacggc ggtccggcta gaccgaaagg
     1621 gacgccgagg ccaagctgcc ctccacgggc aggccgttct catgccattc ctccatgccg
     1681 cccgggtagt cctcggcccg gaagcccttt tccttgagga accgggccgc ctcggggccg
     1741 gcggaacaac cggggcccgc gcaataggtg atgaaaaact tgtcccggcc gaaggcttcc
     1801 gccccccggg cgaaggcttc ttggccgtcc ccccagggga ggttccggga atgtttgatg
     1861 tggagtttgg cgtagtcccc cgcgggcagc acattcagga tgaccacccc gtccgacttc
     1921 agctccttca agacctgctc tttcgtgaag gccatgacgc cctcctttca tcccttgatc
     1981 ctgtttagac acctcaagct aggaaaaggg actgaaggaa gcgcgaaagg aaggaaaaat
     2041 ccaggttaca tttgtgaacg ccgtccggac ggtcggtcgt tctttgtttt taaagaaggc
     2101 cgtgcttctt gaggatgggg aaaacctttt catcgacctt gggatccatg gtgagctcgt
     2161 cgggccattc gcggccgaag ccctcttcct tccatttgcg ggtggcgtcg atgcccagct
     2221 tcccgcccca gccccattgg ggcgaggcgt ggtccagcac gtccaggggg ccctccacga
     2281 tgaccgtatc gcgcttggcg tccatgcagt tgcccacccg ccacagcacc tggttgtagt
     2341 cgtgtacgtc cgtgtccttg tcgaccacca ccaggacctt gttgagcatc atctggccca
     2401 ggccccagag ggcgtacatg accttgcggg cctgctgggg atattgcttg tcgatggaga
     2461 cgatggccag gttgtggaaa atgcccgcca ccggcatgtc taggtccgtg atctcgggca
     2521 gcagtttctg cagcaggggc agaaacaggc gttcggtggc cttgcccagc cagccgtcct
     2581 ccatgggggg cttgcccacc agggtggtgg gataaaccgg cgccttgcgg cgcgtgacgc
     2641 aggtgacgtg gaaaaccggg tagtcgtccg ccaggctgta gtagcccgtg tggtcgccga
     2701 agggcccttc ccggcggcgt tcgccgggtt ccacgtagcc ttccaacacg aagtcggcgt
     2761 cggcgtgcac ccacaggtcc accgtcttgc acttcaccag ctccaccggc ttcttgcgca
     2821 ggaatccggc gaagatcagc tcatccacgc cctcgggaag cggcgcggtg gccgcatagg
     2881 tgagggccgg atcgccgccc aaggccaccg cgacctccag gcgcttgccc atcttctcgg
     2941 ccttgcggtg gtgggccgcc ccgccgtggt gcacctgcca gtgcatgccc gtggtcttgg
     3001 agtcgtggac ctgcatgcgg tacatgccga tgttgcgcac ccccgtctcg gggtcctggg
     3061 tgtagaccag gggcagggtg atgaagcggc cgccgtcctg cggccagcat tgcatcacgg
     3121 gaaggctcaa gaggtccggg tccttctcca ccacttcctg gcaggcgccc ttggaagcct
     3181 ccaccggcgg gaacttggcg atctcggcca gtttgggcag gaacttgagt ttttccacca
     3241 gccccgcagg gggcttcaaa tccagcatgt cccggatggc ggcggcgtgt tgttccaggt
     3301 cctccacgcc cagggcccag gccatgcgtt gcttggaacc gaaggcgttg atgagcaggg
     3361 gataggcgct ccccttgggg cgctcgaaga gcagcgcctt gcccccgccg ggcgatttgg
     3421 agacgcggtc caccagggcg gggatctcca gcaccgggtc cagttcctcg gggatgcggg
     3481 ccagttcccc cttcttctcg aggaatgcca cgaaggcgct cagggactca taggccattt
     3541 aacgttcctt gggcgggggc gcgaaggtcc ccgcgattac cagcccgccg cccagggcgg
     3601 cggaaaccga gaaccacagc ccgggttgaa gccacaggtt catcgccgac tcataaagga
     3661 agaaccagcc
//
LOCUS       DASTJJ010000490         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_314658, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000490 DASTJJ010000000
VERSION     DASTJJ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314658"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFR02_06030"
     CDS             complement(<1..187)
                     /locus_tag="VFR02_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870038.1"
                     /translation="MAQAQTFKQQVVNTASEAVDAGLFWRGNLAKAVRRRDFRGTVWG
                     VAALAFFLFLYVWQHMQV"
     gene            complement(187..>1018)
                     /gene="rsmH"
                     /locus_tag="VFR02_06035"
     CDS             complement(187..>1018)
                     /gene="rsmH"
                     /locus_tag="VFR02_06035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9870039.1"
                     /translation="AAEILKRLPRGRYVGLDQDADALQHAGRALSGDPRVTLHQANFA
                     DLLDVLTTLGIPKVDSVLMDIGVSSLQLDTAARGFSFQQSGPLDMRMDASAPLSAADV
                     VNRYPEEKLAQILREYGEEPKARALARAIVKARAEKPFQTTDELAALAKRVLYRPGPQ
                     KHPATRLFQALRMEVNGELKALERGLEAARQALKPGGRLAVITFHSLEDRLVKQFMKR
                     ESVDCLCPPDLPLCRCGHRASLEILTPKPVTASQAELESNPRARSAKLRVARRLGEAA
                     "
BASE COUNT          166 a          326 c          361 g          165 t
ORIGIN      
        1 ccacctgcat gtgctgccag acatagagga aaaggaaaaa ggcgagcgcc gccacgcccc
       61 agaccgtgcc ccggaaatcc ctacggcgca cggccttggc caggttgccg cgccagaaaa
      121 gcccggcgtc cacggcctca ctggcggtgt tcaccacctg ctgcttgaag gtctgggctt
      181 gggccatcag gcggcctctc ccagcctgcg ggccacccgc aacttggcgc tgcgcgcccg
      241 ggggttggac tccaactcgg cctgcgaggc cgtgacgggc ttgggcgtga ggatctccag
      301 ggaggcccgg tggccgcaac ggcagagggg caggtcgggc gggcagaggc aatccacgct
      361 ctcgcgcttc atgaactgct tgaccaagcg gtcttccagc gaatggaagg tgatgacggc
      421 cagccggccg ccgggcttga gcgcctgacg ggcggcctcc agcccccgct cgagcgcctt
      481 caattccccg ttcacttcca tgcgcagggc ctggaaaagc cgggtggccg ggtgcttctg
      541 ggggccgggg cggtagagca cgcgcttggc caaggcggcc agttcatcgg tggtctggaa
      601 gggtttttcc gcgcgggcct tcacgatggc ccgggccagg gcgcgggcct tgggctcctc
      661 gccgtattcc cggaggatct gggcgagctt ttcctcggga tagcggttga ccacgtccgc
      721 cgccgaaagg ggcgcggagg cgtccatgcg catgtcgagg gggcccgatt gctggaagga
      781 aaaaccgcgg gccgcggtgt ccaattggag cgaagatacg ccgatgtcca tcaacacgct
      841 gtccaccttt gggatcccaa gggttgtcag cacatccagg aggtcggcga agttagcctg
      901 atggagcgtg acccgggggt cgccgctcag tgctcggccg gcgtgttgaa gggcatcggc
      961 atcctggtcc aaccccacgt aacggccgcg gggcaggcgt ttcaaaatct ccgctgcg
//
LOCUS       DASTJJ010000491         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1414457, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000491 DASTJJ010000000
VERSION     DASTJJ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414457"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFR02_06040"
     CDS             <1..330
                     /locus_tag="VFR02_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870040.1"
                     /translation="AKFWLDRDTALQPACKFGWGGNGALECDLLWHNYRWIPSQGDEW
                     PVYAGFGAALDTAGPWVGGRGVVGVAYLLEDLPGDFFVQLVPTYWFGGAPAAFQLYGE
                     TGLRLFL"
     gene            369..>1017
                     /locus_tag="VFR02_06045"
     CDS             369..>1017
                     /locus_tag="VFR02_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET9870041.1"
                     /translation="MVSSKSRSKPDLMMYAASEHDSNLYYAVKFSVPDPFPYFRIGGK
                     DIILMSDLELGRAKKQAKVREVLSYSDWQLKVRAQGVERPSVADVAAAFLKAHRVKAV
                     TVPDNFNLGLADALRRRGIGVESKPDPFFEERVLKDAEEVRLITRSLRHTEKAIHRAE
                     DLLRRSRIVGTRIFHRGTLVTSELLKKVINVSMMEDNCVGSHTIVAGGDQGCDPHQ"
BASE COUNT          179 a          312 c          333 g          193 t
ORIGIN      
        1 gccaagttct ggctcgaccg ggacacggcc ctccaaccgg cctgtaagtt cggttggggc
       61 gggaatgggg ccctggaatg cgaccttctt tggcataatt accgctggat cccgtcccaa
      121 ggggatgagt ggccggtcta cgccgggttc ggcgccgcct tggacaccgc cggtccctgg
      181 gtggggggcc ggggcgtggt gggggtggcc tacctgttgg aagatttacc gggtgatttt
      241 ttcgtccaac ttgtgccaac ctattggttc ggcggggcgc cggccgcttt ccagctctat
      301 ggggaaacag gcctgcgcct ctttctttga gcggcccgcg ggggcccgtc caataacgag
      361 ggggtcccat ggtttccagc aaaagccggt ccaagcccga cttgatgatg tacgcggcca
      421 gcgagcatga ttcgaacctc tactatgccg tgaagttctc cgtgcccgac cctttcccct
      481 atttccgcat cggcgggaag gacatcatcc tgatgagcga cctggaactg ggacgcgcca
      541 agaagcaggc caaggtccgg gaggtgcttt cctactcgga ttggcagctc aaggtccgcg
      601 cccagggcgt ggaaaggccc tcggtggccg acgtggccgc ggccttcctc aaagcccacc
      661 gggtgaaggc cgtcaccgtg ccggataatt tcaacttggg cctggccgat gccttgcgcc
      721 gaaggggcat cggggtggag agcaaacccg accccttttt cgaggaacgg gtcctcaagg
      781 acgccgaaga agtgcggctc atcacccggt ccctgcgcca taccgaaaaa gccatccatc
      841 gggccgagga ccttctgcgg cggtcgagga tcgtgggcac gcgtatcttc caccggggaa
      901 ccctggtcac ttccgagctt ttgaagaagg tgatcaacgt ctccatgatg gaggacaact
      961 gcgtcggcag ccacaccatc gtggccggcg gcgatcaggg ctgcgacccg caccagg
//
LOCUS       DASTJJ010000492         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_436464, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000492 DASTJJ010000000
VERSION     DASTJJ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436464"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1282
                     /gene="hemW"
                     /locus_tag="VFR02_06050"
     CDS             173..1282
                     /gene="hemW"
                     /locus_tag="VFR02_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET9870042.1"
                     /translation="MNAYLHVPFCASICAYCDFTSFAGRESKTGLYVRALAREIEGSD
                     LAGPLGTVYFGGGTPSLLSPDELGTVLGALRGKAGMAPGAEVSLEANPETASLEKFRA
                     YRELGVNRLSFGAQASQNPVLRRLGRGHDWARVERAFNQAREAGFDNLNLDLMIGLPG
                     QTRGDFEATLRQAAALSPDHLSVYALQVEEGTPLSRQVAEGLALPSEDEVADEYAFAQ
                     GFLAERGLDQYEVSNFAREGKACRHNWNVWRGEDYWGFGLSAVGTVRGVRHSHDEDLE
                     AYLRTADAGTPRVTRETLTPAERHFERLMLGLRTREGVGRDDLEGYARAQGLAAGERF
                     RQFQDRGWLEEAEGRFRVTSRGYFVLNGLLEALVA"
     gene            1378..2418
                     /gene="hrcA"
                     /locus_tag="VFR02_06055"
     CDS             1378..2418
                     /gene="hrcA"
                     /locus_tag="VFR02_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET9870043.1"
                     /translation="MAETAPNRNETVLSTVIHRYVATAKPVASFSLSGQIRVSPATIR
                     NVFAALEDRGYLTHPHTSAGRVPTDEGYRFYVDRLMRARELNLREKRRIEEEYGRAKD
                     EVEALMRHTAKILSAMTRLGGLAVFHPEDGAQLDHFKLVSLDPRKVMVILVLTNGLVE
                     EELVRLESPLPAGELARVTHLLNGKLKGLSLGVLRRELLSDAERLRDRQLGLVQAALK
                     TIDDALDLEGESIQLEGLSQLMEQPEFKTLENLERLVRLVEERRALRPLLDRQWSRPG
                     LAVEIGEDSGLGAPGKNLSFMHLPYRLKGRVAGLLGVWGPTRMDYGRVAGLMGQVARS
                     LEEALNTRGGWS"
     gene            2418..3014
                     /gene="grpE"
                     /locus_tag="VFR02_06060"
     CDS             2418..3014
                     /gene="grpE"
                     /locus_tag="VFR02_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9870044.1"
                     /translation="MPDMNQNAGEPEIEILDSQAIPYDDAGNASDLPVEEPVPPGAAA
                     AAGEAKEWKKKHDEVKEQLLWTAAEFDNYKKRALKEKDEFLKFAQAGLLKEFLVVADN
                     LERAVASTAGSDGTLGTLKQGVELTLRQFQAVMEKYGVTRIKAKGEVFDPRAHEVMLQ
                     EETDQAPEDTVLEELQAGYRLHDRVLRPALVKIAKKKA"
     gene            3123..>3398
                     /locus_tag="VFR02_06065"
     CDS             3123..>3398
                     /locus_tag="VFR02_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9870045.1"
                     /translation="MATNKVLGIDLGTTNSCMAIMEGGEPVVIANAEGARTTPSVVGF
                     SKSGERLVGRVAKNQAITNPTNTVFSVKRFMGRKLHDPEVEVDKKLVP"
BASE COUNT          608 a         1097 c         1200 g          493 t
ORIGIN      
        1 actcccgcta ttggggccag ctggacgaga ggctcatcaa gggccaggcc tgggtcatct
       61 attggcacag cgtgaacttc catcccgacc tgagccggat gttccaccgg gtgctgaccg
      121 agtcgcccga acccgcgccc acgcccgtgc cggtcccgag gaaaaagaag ccgtgaacgc
      181 ctacctgcac gtccccttct gcgcgtccat ctgcgcctat tgcgatttca cgtccttcgc
      241 ggggcgggaa tccaagacgg gcttgtacgt ccgggccctg gcgcgggaga tcgaagggtc
      301 cgacctggcg gggccgctgg gcacggtcta tttcggcggc ggcacgcctt cgctgctctc
      361 gcccgatgag ttggggacgg tcctgggcgc cctgcgcggc aaggcgggga tggcgccggg
      421 ggccgaggtg tcgttggaag ccaatccgga gaccgcctcc ctggaaaaat tccgggccta
      481 ccgggaactg ggcgtgaacc gcctcagctt cggggcacag gcctcccaaa acccggtgct
      541 gcgccggctg ggccgggggc acgactgggc gcgggtggag cgggccttca accaggcccg
      601 ggaagcgggc ttcgacaacc tgaacctgga cctcatgatc ggcctgccgg gccagacgcg
      661 gggggatttc gaggcgaccc tgcgccaagc cgccgcgctt tcgcccgacc acctgtcggt
      721 ctacgccctg caggtggagg agggcacgcc cctttcccgg caggtggcgg aaggcctggc
      781 cctgccctcc gaggacgagg tggccgacga atacgccttc gcccagggtt tcctggcgga
      841 gcgggggctg gaccaatatg aggtgtccaa cttcgcccgg gaagggaagg cctgccggca
      901 caactggaac gtgtggcggg gggaggacta ctggggattc ggcctttcgg ccgtgggcac
      961 ggtgcggggc gtccggcaca gccacgacga ggacctggaa gcctacctgc gcaccgccga
     1021 cgccgggacg ccccgcgtca cgcgggagac cctcacgccc gccgagcggc atttcgagcg
     1081 gctgatgctg ggcctgcgca cccgggaggg cgtgggccgg gacgacctgg aaggctacgc
     1141 ccgggcccag ggcctggccg ccggggaaag gttccgccag ttccaggacc ggggatggct
     1201 ggaggaggcc gaggggcgtt tccgagtgac ctcccggggc tatttcgtgc tcaacgggct
     1261 cttggaagcc ctggtggcct gagccttccc gcgccgggaa ggcaagttga ctttcaagac
     1321 cctcatccta tgatgaggat gcatccgaag cgcagacccc gtccaaaggc ttgaaaagtg
     1381 gctgaaaccg cgcccaaccg aaacgagacc gtcctgtcga ccgtgatcca ccggtacgtg
     1441 gccacggcca agccggtggc ttccttttcc ctgtccggcc agatccgggt ctccccggcc
     1501 accatccgca acgtcttcgc ggccctggag gaccggggct acctgaccca tccccacacc
     1561 tcggcgggac gggtgcccac cgacgagggc taccgttttt acgtggaccg gctcatgcgg
     1621 gcccgggagc tgaacctgcg ggaaaagcgc cgcatcgagg aagaatacgg ccgggccaag
     1681 gacgaggtgg aggcgctcat gcgccacacg gccaagatcc tgtcggccat gacccggctg
     1741 ggggggctgg cggtgttcca ccccgaggac ggcgcccaac tggaccactt caagctggtg
     1801 tccctggacc cgcgcaaggt catggtcatc ctggtgctca ccaacggcct ggtcgaggaa
     1861 gagctggtcc ggctggaaag ccccctgccc gccggggaac tggcccgggt gacgcacctg
     1921 ctcaacggca agctcaaggg cctgtccctg ggcgtcctgc gccgcgagct gctctccgac
     1981 gccgagcgcc tgcgggaccg ccaactgggc ctggtgcagg cggcgctgaa gaccatcgac
     2041 gacgccctgg acctggaggg ggaaagcatc caactggagg gcctttccca gcttatggaa
     2101 cagcccgagt tcaagaccct ggagaacctg gagcgcctgg tgcgcctggt ggaggaacgc
     2161 cgggccctgc ggcccctgct ggaccgccag tggagccgcc ccgggctggc ggtggagatc
     2221 ggcgaggatt cggggttggg cgcgccgggc aagaatcttt ccttcatgca cctgccctac
     2281 cggctgaagg gccgggtggc gggcctgctg ggggtctggg gcccgacccg gatggattac
     2341 ggccgggtgg cggggctcat gggccaggtg gcccggtccc tggaagaagc cttgaacaca
     2401 cgaggaggat ggtcctgatg ccggacatga accaaaacgc gggcgagccc gagatcgaga
     2461 tcctggacag ccaggccatc ccctatgacg acgcgggcaa cgcctccgac ctgcccgtgg
     2521 aggaacccgt gccccccggg gccgcggccg ccgccggcga ggccaaggaa tggaagaaga
     2581 agcacgacga ggtgaaggag cagctgctct ggaccgccgc cgagttcgat aattacaaga
     2641 agcgggcctt gaaggagaag gacgaattcc ttaaattcgc ccaagccggg ctcctcaagg
     2701 agttcctggt ggtggccgac aacctggaaa gggcggtggc ctccaccgcc gggtccgacg
     2761 gcaccctggg caccctcaag cagggcgtgg agctgacgct gcgccagttc caggccgtga
     2821 tggaaaagta cggcgtcacc cgcatcaagg ccaagggcga ggtcttcgac ccccgggccc
     2881 acgaggtgat gctgcaggaa gagacggacc aagcccccga ggacacggtc ctggaggaac
     2941 tccaggcggg ctaccgcctg cacgaccggg tgttgcgccc ggccttggtg aagatcgcca
     3001 agaagaaggc ttgaaacccc gcggggcttc atgacggggg acatacaagc gggcggaaca
     3061 agggtttaac atcatcccca ggggatcccg ggggcatttt taggaaatga aggagaacgc
     3121 atatggcgac gaacaaggtg ttgggcatcg atctgggcac caccaattcc tgcatggcga
     3181 tcatggaggg cggcgagccc gtggtgatcg ccaacgcgga gggcgcccgc accaccccct
     3241 cggtggtggg cttttccaag agcggcgagc ggctggtggg ccgggtggcc aagaaccagg
     3301 ccatcaccaa ccccaccaac acggtctttt cggtgaagcg tttcatgggc cgcaagctgc
     3361 acgaccccga ggtggaggtg gacaagaaac tggtcccc
//
LOCUS       DASTJJ010000493         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1660093, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000493 DASTJJ010000000
VERSION     DASTJJ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660093"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFR02_06070"
     CDS             <1..223
                     /locus_tag="VFR02_06070"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET9870046.1"
                     /translation="VYGNRGLLRLYQLHAQKSALTGQIAQLQAQITDYQSEYKRFNQD
                     PSAIEKRAREELNLVKPGEIIYRFEAPGR"
     gene            401..488
                     /locus_tag="VFR02_06075"
     tRNA            401..488
                     /locus_tag="VFR02_06075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:435..437,aa:Leu,seq:taa)
     gene            527..603
                     /locus_tag="VFR02_06080"
     tRNA            527..603
                     /locus_tag="VFR02_06080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:561..563,aa:Met,seq:cat)
     gene            633..866
                     /locus_tag="VFR02_06085"
     CDS             633..866
                     /locus_tag="VFR02_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HET9870047.1"
                     /translation="METVHLRLETHGRRGKAVTVLQGFTRRPEELEALARTLKAKCGS
                     GGTVKGSMIEIQGDVRARVRDLLLAEGFHVKGV"
     gene            868..1668
                     /locus_tag="VFR02_06090"
     CDS             868..1668
                     /locus_tag="VFR02_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870048.1"
                     /translation="MRVSASVSPWLPWLEPFLPSLVLLGGSASELYHSSLLGRPIPPL
                     LIKEKVFGLEKAGKASGALHEHLVKSGFQKRNERPADAAGPVPAYFRGDLGTLRFVFP
                     QTRAGQTASSGGVAAMPEKGLELLLENPHAVEVKYLDRTYDVRVPQIGRFVLANGLKV
                     RAPKKAGPAEVYQAAMRLILVLDLLAGSEDFEDEALDDLLEIRPPALVRELRDNLKDN
                     GPGSILWESAQRLYLNLYPGAKAVELATWHWKFQKRLSKNITDARSAE"
     gene            1679..>1964
                     /locus_tag="VFR02_06095"
     CDS             1679..>1964
                     /locus_tag="VFR02_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9870049.1"
                     /translation="MPKMKKKRTGRPLALVTGGASSLGGAISRTLAQAGYDLVLHYGR
                     SQGAARRLRAELEAAGGRVTLLQADLGRPAALARLLRPRARDFARLSLLVN"
BASE COUNT          362 a          643 c          606 g          353 t
ORIGIN      
        1 cgtttacggg aaccggggat tgctgaggct ctaccaactg cacgcccaaa agagcgccct
       61 cacgggacag atcgcccaac tccaggccca gatcacggac tatcagagcg agtataaacg
      121 tttcaaccaa gacccctccg ccatcgaaaa aagggcccgg gaagaattga acttggtaaa
      181 accgggcgaa atcatctacc gcttcgaggc cccaggccgc tgacatccct tcctttccac
      241 tgattgacgc ttgacttccg gacagggggt cattatcctg ccccttcttg gtagggccat
      301 ccggttcgtc ccacgattcg ctcttctggt ccgtggcacc ccttcgagct gataaggtca
      361 tccaggtttc tcggggtatt catcccggga acgggtgagc gccgagatgg cggaattggc
      421 agacgcaagg gacttaaaat ccctcgggcc taaagcccat cccggttcga tcccgggtct
      481 cggcaccaat tttaagtcgt gagttccgca gtggcagtga aacagacgcg gggtggagca
      541 gcctggtagc tcgttgggct cataacccaa aggtcgctgg ttcaaatcca gcccccgcaa
      601 ccattttttc cccttccgga agccttcccc ctttggaaac cgtccacctt cgtttggaaa
      661 cccatggccg tcgcggcaaa gccgtcaccg tcctccaggg attcacccgt cgtcccgaag
      721 aactggaagc cttggcccgc acgctcaagg cgaaatgtgg ttccggaggc accgtgaaag
      781 gaagtatgat tgagatccaa ggcgacgtca gggccagggt ccgcgacctg ctgctcgccg
      841 aaggattcca cgtaaagggc gtctgacttg cgcgtgtccg cttccgtctc cccgtggctg
      901 ccttggctgg agcctttcct gccttccttg gtgcttttgg gaggcagcgc ctccgaactc
      961 taccacagct cgcttttggg ccgtcccatc cccccgctgc tcatcaaaga aaaggtcttc
     1021 ggcctggaaa aggccggcaa ggcctccggc gcgctccacg agcacctggt gaagtccggc
     1081 ttccaaaaac gcaatgagcg gcccgcggac gccgccggcc ccgtgccggc ctatttccgg
     1141 ggcgacctgg ggacgctgcg ttttgtgttt ccccagaccc gcgccggcca gacggcttcc
     1201 tcgggcggcg tggcggccat gcccgaaaag gggttggagc ttttgctgga aaatccccac
     1261 gcggtggaag tgaagtacct ggaccgcacc tacgacgtgc gggtgcccca gatcggccgg
     1321 ttcgtcctgg ccaacgggtt gaaggtgcgg gcgcccaaga aggccgggcc cgcggaggtc
     1381 tatcaggcgg ccatgcggct catcctggtc ctggacctcc tggcgggcag cgaggatttc
     1441 gaggatgagg cccttgacga cctgttggag atccgccccc ccgccctggt gcgggaactg
     1501 cgggacaacc tcaaggacaa cggcccgggg tccatcctgt gggaatcggc ccagcgcctc
     1561 tacctgaacc tgtatcccgg cgccaaggcg gtggaactgg ccacttggca ctggaaattc
     1621 cagaagcgct tgtccaagaa tatcaccgac gcccgatcgg cggagtgaag ggggggccat
     1681 gcccaaaatg aaaaagaaaa ggaccggccg gcccctggcc ctggtcacgg gcggcgccag
     1741 cagtttgggc ggcgccatca gccggaccct ggcccaagcg ggttacgacc tggtcctcca
     1801 ttatggccgg tcccaagggg ccgcgcgcag gctccgggcc gaactggagg ccgccggcgg
     1861 ccgggtgacc ctgctccaag cggacctggg ccgtccggcg gcgctggccc gtctcctgcg
     1921 gccccgggcc cgggacttcg cgcgcttgag cctgctggtg aaca
//
LOCUS       DASTJJ010000494         1971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1414668, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000494 DASTJJ010000000
VERSION     DASTJJ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414668"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="ilvA"
                     /locus_tag="VFR02_06100"
     CDS             <1..1047
                     /gene="ilvA"
                     /locus_tag="VFR02_06100"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HET9870050.1"
                     /translation="LEILRQHPGPLDAVFVAVGGGGLIAGLAAYIKQLRPEVKVIGVE
                     PEDSDAMGRSLRAGRRVTLSHVGLFADGVAVKRVGRETFRLCRQWVDGMLTVDTDDIC
                     AAIKDVFEDTRSILEPAGALALAGLKQYAEKHRLRGRTLAAVACGANMNFDRLRFVAE
                     RAELGEHREALLAVTIPERPGSFRAFCALLGRHNVTEFNYRYADPRQARVFVGLQVKG
                     RRETAALKARLRQAGLPALDLSLNEMAKLHVRHLVGGRAPGLTNERLFRFEFPERPGA
                     LRAFLTRMGAGWNISLFHYRNHGADYGRVLAGLQVPPGEGAKFKAFLAGLGYAWVEET
                     ANPAGTLFLGSPGQ"
     gene            complement(1100..1438)
                     /locus_tag="VFR02_06105"
     CDS             complement(1100..1438)
                     /locus_tag="VFR02_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9870051.1"
                     /translation="MKKIEAIIRPSALKTVLDGLNKVGYPGVTVVPVQGHGKQKGITE
                     SYRGQTVEGLLPKLMVIAVVEDGQVEEVIETITKAAKTGEIGDGKIFVIDIANAFRIR
                     TGEKGNKAIS"
     gene            complement(1478..>1971)
                     /locus_tag="VFR02_06110"
     CDS             complement(1478..>1971)
                     /locus_tag="VFR02_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET9870052.1"
                     /translation="AIMWNIVELLHKGKATTLGAASGAVAGLVAITPACGYVDLVGSL
                     WVGGVVSIVCYLAIQVKIKLGYDDTLDTFGVHGIGGTWGAIATGIFAVKAINGSSGWL
                     DGNPAQMLIQLKTVGFTAIFSLFGTAVLYFLVEKTIGLKASVKEEELGLDLSQHGEEG
                     YIL"
BASE COUNT          321 a          673 c          622 g          355 t
ORIGIN      
        1 ctggagatcc tccgccagca ccccggcccc ctggacgcgg tcttcgtggc cgtgggcggg
       61 ggcgggctca tcgcgggcct ggccgcctac atcaaacagc tgcggcccga ggtcaaggtc
      121 atcggcgtgg aacccgagga ctcggacgcc atgggccgct cgctgcgggc cggacgccgc
      181 gtcacccttt cccacgtggg ccttttcgcc gacggcgtgg cggtgaagcg ggtcggccgg
      241 gagaccttcc ggctctgccg ccagtgggtg gacggcatgc tgaccgtgga tacggacgac
      301 atctgcgcgg ccatcaagga cgtcttcgag gacacccggt ccatcctgga acccgcgggc
      361 gccctggccc tggccgggtt gaaacaatac gccgaaaagc accgcctgcg gggacgcacc
      421 ctggcggcgg tggcctgcgg cgccaacatg aatttcgacc ggctgcgttt cgtggccgag
      481 cgggccgaac tgggcgagca tcgggaggcg cttttggccg tcaccatccc ggaaaggccc
      541 ggcagcttcc gggccttctg cgccctcttg ggccggcaca acgtgaccga attcaactac
      601 cgctacgccg acccccggca ggcccgggtc ttcgtggggc tccaggtcaa gggccgccgg
      661 gagaccgccg cgttgaaggc ccggctccgc caagccggac tgcccgccct ggacctgagc
      721 ctcaacgaga tggccaagct ccatgtgcgc cacctggtgg gcggccgggc gccgggcctg
      781 acgaacgagc gccttttccg gttcgagttc cccgagcgcc ccggcgccct ccgggccttc
      841 ctcacccgca tgggcgcggg ctggaacatc agcctgttcc actaccgcaa ccatggcgcg
      901 gactacgggc gggttttggc gggcctccag gtgccgcccg gcgagggggc caagttcaag
      961 gctttcctgg ccggcttggg ctacgcctgg gtggaggaga cggccaaccc ggccggcacc
     1021 ctcttcctgg gaagtccggg acaataaaaa aggggagggg gctttcgccc cctccccttc
     1081 ctctctccga cagggccgat taactgatgg ccttgttgcc tttttcgccg gtacggatgc
     1141 ggaaggcgtt cgcgatgtcg atgacgaaga tcttcccgtc gccgatctcg ccggtcttgg
     1201 cggccttggt gatcgtctcg atcacctctt ccacctgacc gtcctccacc accgcgatca
     1261 ccatcaactt gggcagcagc ccttccacgg tctgcccgcg gtaggactcg gtgatgccct
     1321 tctgcttgcc atgcccctgc accgggacca ccgtgacgcc ggggtatccc actttgttca
     1381 atccatccag cacggtcttc aaagcgctgg gacggataat cgcttcaatt tttttcattt
     1441 cgttccctcc atcatgggtg agtgaccaac gctttgttta gagaatataa ccttcttcac
     1501 catgctggga caagtcgagg cccagttcct cttctttcac ggaagccttc agaccgatgg
     1561 tcttttcgac caggaagtac aggactgcgg ttccgaacaa cgagaagatc gcggtgaagc
     1621 ctacggtctt cagctggatg agcatctggg cggggttgcc gtccagccag cccgagctgc
     1681 cgttgatggc cttgacggcg aagatgccgg tggcgatggc accccaggtg ccgccgatgc
     1741 cgtgcacgcc gaaggtgtcc agcgtgtcgt catagcccag cttgatcttc acctggatgg
     1801 ccaggtagca gacgatggag accacgccgc ccacccaaag cgaacccacc aagtcgacat
     1861 aaccgcaagc cggggtgatg gccaccagac cggccaccgc gcccgaggcc gcgcccaggg
     1921 tcgtcgcctt gcccttgtgc agcaattcga cgatgttcca catgatggcg c
//
LOCUS       DASTJJ010000495         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1782617, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000495 DASTJJ010000000
VERSION     DASTJJ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782617"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFR02_06115"
     CDS             <1..268
                     /locus_tag="VFR02_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870053.1"
                     /translation="EDNKARRIATITACNDGSNSSWRARLGNGKPGEETYRNKDQTPK
                     KIGQLMQNQHAGRKIQAFCFGSLFHGYAVQNENAADQCGDMLSI"
     gene            complement(348..1253)
                     /locus_tag="VFR02_06120"
     CDS             complement(348..1253)
                     /locus_tag="VFR02_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9870054.1"
                     /translation="MNSRKFLFLLAVGALGLGWGCKNNTTTAPSADHPLDFVVGGDIG
                     PGKNEFSAPMGLCVDGSDNLYVADYGNSRIKQYDSNGACQAVWGGPATVATVAAALNH
                     PWGLALDSAGNILEVDHDTEQIQEFTTAGALVAQYGSFNVATWNAAGPGGVNMVSDDT
                     GVHLFNGSGANGILATPGGSAFGHYALDPSGLAYDAKGDIYVGDLGNHRIDVYDSTGT
                     PVSIWGAGRFSSIAGMALDRSSNLLVADAGSRLIFKFDKFGNFVESWMPDTVAQPMDV
                     AVDSTGAIWVSFQDVSQAFLAKFAH"
     gene            complement(1423..>2073)
                     /locus_tag="VFR02_06125"
     CDS             complement(1423..>2073)
                     /locus_tag="VFR02_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HET9870055.1"
                     /translation="VELINGVASEVKADVYLPPATDGAGNATLPANSPLPTIGANITQ
                     IQMNFGTAGILGQGKRDGLFSDAEGTFQIVNGVNTYVPSDTATVKSQDGYADGTLQSL
                     YFDKTGTIQGVFSNGKTFALGQVVLTSVTNQEGLQSVGNGYYSQTASSGPSYTGLAGT
                     ANLGTVQGSALESSNVDLTVELSNMIIAQRGYESNARVISVVNSTLQTLTQLGQGA"
BASE COUNT          433 a          637 c          592 g          411 t
ORIGIN      
        1 cgaggacaat aaggcccggc gaatcgccac catcaccgct tgcaatgacg gaagtaatag
       61 tagttggaga gcaaggttgg gaaatggcaa gccgggggaa gaaacatacc gcaacaagga
      121 ccaaacacca aaaaaaatcg gccagttgat gcaaaaccaa cacgcgggcc ggaaaattca
      181 agcgttctgc ttcggctctc tttttcatgg atacgccgtt caaaatgaaa atgccgccga
      241 tcaatgtggc gacatgctat caatctaaaa aatgacggtc cactccaaag taaaaagcga
      301 ggaaaattaa atcgttattc ggtttaacga tttttaaatt ttaacagtta atgtgcaaat
      361 ttagccagaa aagcctggct tacgtcttgg aagctcaccc aaatcgcccc cgtgctgtcg
      421 acggcaacgt ccatgggttg agccaccgtg tccggcatcc aactttccac gaagttcccg
      481 aacttgtcga acttgaagat cagccgggat ccggcgtccg ccaccaaaag gttggaagac
      541 cggtcaaggg ccatccccgc gatcgatgaa aaacggcccg caccccagat ggaaaccggc
      601 gtcccggtgc tatcgtacac gtcgatccgg tggttgccga ggtcccccac atagatgtcc
      661 cccttcgcgt cataggccag gccggatggg tccaaggcgt aatggccgaa agcgctgcct
      721 cccggggtcg ccaggatgcc gttcgcgcca gaaccgttga acaaatggac ccccgtgtcg
      781 tccgagacca tgttgacgcc gcccgggccc gcggcgttcc aggtcgccac gttgaaactt
      841 ccgtattgcg cgaccagggc ccccgccgtc gtgaactcct ggatctgttc ggtgtcatgg
      901 tcgacttcca ggatgttgcc cgccgaatca agggccaatc cccagggatg gttgagggcg
      961 gcggccaccg tggccaccgt ggcgggccca ccccagaccg cttggcaggc accgttcgag
     1021 tcgtattgct tgatccggct gttgccgtaa tcggccacgt agaggttgtc cgagccgtcc
     1081 acgcaaagcc ccatgggggc gctgaattcg ttcttccctg gcccgatatc gccgcccacc
     1141 acaaaatcca gcggatggtc cgcgctgggg gcggtcgtgg tgttgttctt gcaaccccaa
     1201 cccaggccga gggccccgac cgccaatagg aataaaaatt tccgagagtt cattttccac
     1261 ccaagtcacc gccggccaag ccggggttca ttgacaaaag cccaaccgat cccaccttca
     1321 cattttaccg cgtccgaaaa aaagaaaggc gacgggtttt atccggcgtc gcctttccat
     1381 cctagcgggg cttgcgcccc ctaactttta ttcaagctgc gatcaagccc cttggcccag
     1441 ttgcgtcaag gtctgcaggg tggagttgac gaccgagatg acccgggcgt tcgactcgta
     1501 gccccgctgg gcgatgatca tgttggacag ttccaccgtc aggtcgacgt tggagctttc
     1561 caacgcgctt ccctggacgg tacccaggtt ggccgtgccg gccaatcccg tgtaggacgg
     1621 tcccgagctg gcggtctggc tgtagtagcc gttccccacg ctctgcaggc cctcctggtt
     1681 ggtgaccgac gtcaagacca cttggcccag cgcgaaggtc ttgccgttgg agaacacgcc
     1741 ctggatcgtg cccgtcttgt cgaagtacag gctctgcagc gtgccgtcgg cgtagccgtc
     1801 ctgcgacttg accgtggcgg tgtcgctggg cacgtaggtg ttgaccccgt tcacgatctg
     1861 gaaggtgcct tccgcgtcgc tgaacaggcc gtcccgcttg ccctggccca agatgccggc
     1921 ggtgccgaag ttcatctgga tctgggtgat gttggccccg atggtcggca ggggcgaatt
     1981 cgccggcaaa gtcgcgttac cggccccgtc cgtcgcgggc ggaagataaa catccgcctt
     2041 gacctcgctg gcaaccccgt tgatcagttc cac
//
LOCUS       DASTJJ010000496         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1049558, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000496 DASTJJ010000000
VERSION     DASTJJ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049558"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFR02_06130"
     CDS             <1..771
                     /locus_tag="VFR02_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870056.1"
                     /translation="LAVGSADLSIGRLWPSGLVFAAPFLTAALVGAAGVLFRKPRPWL
                     LGSVYLGWVWVETSAAGFTPASYQAWLPPLVLGAAWGAWEWGAGKRPWAAVLPAVLLI
                     LCALGEEDPYYRLWASQWSGLKSGVDAAGTYALARDLKDMLGPGETFFEWGDETQLYY
                     LTRQTPPSGILECTPAITGPLAGRLGDRLAADLKRAKPDLFLVNRAETREVGPSDPVA
                     ELLRREYRPLGAKPAPGDILFFVRVGSPLDRRYGGTRP"
     gene            complement(768..1889)
                     /locus_tag="VFR02_06135"
     CDS             complement(768..1889)
                     /locus_tag="VFR02_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870057.1"
                     /translation="MTRAARLLLLTAALGSLAPAARAERIMDSVDALTSALSATQTQL
                     ERGDFKNLLANAQKAAQLAQDRFGPLHPSVAPYFDNLGVVEETLAHYPEAETHFEWSL
                     ALREKAGGPDDPALAPALVSLAFLDGQLGRFEEAQVDIQRALALIEAAPDKPGLVGAL
                     QLAGDLQYQAGHPDLAAGFWTRALAERPDPGVRVALLARLGSAEGSQGQLSQARASFQ
                     EALRTAQRSFAPDDIRIADRLKDLGDLDLRSGRGDEGKELLEKALAMDLRFIGPEDTY
                     ANLPYHQRGADAYYSLGQWSQADALYRRILSIKTGVYGPAHPELALTLEDLARTAEKL
                     GDRPGAKKDLADALAILTRYFPDSHPLVVDLKARQAALR"
     gene            complement(1886..>3113)
                     /locus_tag="VFR02_06140"
     CDS             complement(1886..>3113)
                     /locus_tag="VFR02_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870058.1"
                     /translation="TMRLPWITPSLTLLLWSSTLPLWADYGSTFIANSLLSSVIQASD
                     QKMAFRFTSRQDANVIGLSIYCTAAQDPPAYLVSLQEDKDGAPSGVPLGNSSVVPLAG
                     RWITLPVDNIPLAQGKVYDLVIEHNVTRGGFHPVGVIGPKNQAAFGYTDRPNRMDPQD
                     GKADPRLNVLSYEGGKWKALDRQPLFALHEAGDQLQGDPYDDPGSLPIHGNGTPSDPS
                     DDVLGGEALHPHCAFMAKAIRARVRRQGRPDAPLNYRVYTIDYRRHQSSLAFEGEALA
                     PGRASDAFQWITIPLRSADHPQGFPDECRYIVFQTDAGRAVPQAPGCEDCYVLAEAGN
                     SGGLPDACDLTFDGGPHLSREAWSHDGGATWVDAFERDADIQLLGPDCSSMITNSPPQ
                     THPLPTPPSLYRFLRP"
BASE COUNT          465 a          930 c         1181 g          537 t
ORIGIN      
        1 ctggccgtgg gaagcgcgga tctttccatc ggccggcttt ggccttcggg cctggttttc
       61 gccgcgcctt tcctgacggc ggcgctggtg ggcgcggccg gggttctttt ccgcaaaccc
      121 cggccttggc tgctcgggtc ggtctatttg ggttgggttt gggtggaaac ctcggcggcg
      181 ggattcacac ccgcgtccta ccaagcctgg cttccgccct tggtgttggg cgcggcctgg
      241 ggcgcttggg aatggggggc ggggaagcgg ccctgggccg cggtcctgcc ggcggtcctg
      301 ttgatcctct gcgctctcgg cgaagaagac ccctattacc ggctttgggc gtcgcaatgg
      361 tccggtctga agtcgggcgt cgatgccgcc gggacctacg ccttggcgcg cgacctgaag
      421 gatatgttgg gaccaggtga gacctttttc gagtgggggg atgagaccca attgtattac
      481 ctgacccggc agaccccgcc cagcgggatc ctggaatgca cgccggccat cacggggccc
      541 ctggccgggc ggttggggga ccggttggcg gccgacctca agcgggccaa gccggatctt
      601 tttttagtga accgcgcgga gacccgggag gtggggcctt cggaccccgt ggcggagctg
      661 ctgcgccggg aataccggcc gttgggcgca aagcccgccc cgggggacat cctgttcttc
      721 gtacgggtgg gaagcccctt ggaccgaagg tacgggggaa cgcggcctta gcgcagggcc
      781 gcctgccggg ccttcaggtc gacgaccaag gggtggctgt cggggaaata acgggtcagg
      841 atggcgagcg cgtccgccag gtccttcttg gcccccggcc ggtcgccgag tttttcggcg
      901 gtgcgcgcca ggtcctccaa ggtcagggcc agttcagggt gggcggggcc gtagacgccg
      961 gtcttgatgg acaggatccg gcggtagagg gcgtcggcct gggaccattg gcccagggaa
     1021 taataggcgt cggcgccccg ttggtggtag ggcaggttgg cgtaggtgtc ctcgggaccg
     1081 atgaagcgca ggtccatggc cagggctttt tccaggagtt cttttccctc gtccccgcgg
     1141 cccgaacgca ggtccaggtc ccccaggtcc ttcaagcgat ccgcgatccg gatatcgtcc
     1201 ggggcgaaac tacgttgggc ggtccgcagg gcttcctgga aggacgcccg ggcctgggac
     1261 agttggcctt gggatccttc cgcggaaccg agccgggcca agagggcaac ccggacgccg
     1321 ggatccggcc gctccgccag ggcccttgtc caaaacccgg cggccaggtc cgggtggccg
     1381 gcctggtatt ggaggtcccc cgccaattgc agcgccccga ccaggccggg tttgtccggg
     1441 gccgcctcga tcagcgccaa ggcgcgttgg atgtcgacct gggcttcctc gaagcgcccc
     1501 aattggccgt cgaggaaggc caaggacacc agggcgggcg ccaaggcggg atcgtccggc
     1561 ccgccggctt tttcccgcag cgccaaggac cactcgaagt gggtctcggc ttcggggtaa
     1621 tgggcgagcg tttcctccac cacccccagg ttgtcgaaat agggcgccac cgaaggatgc
     1681 agggggccga aacggtcctg cgccagttgg gcggcctttt gcgcgttggc gagcaggttc
     1741 ttgaagtcgc cccgttccaa ctgggtctgg gtggccgata gggcggaggt gagcgcgtcc
     1801 acgctgtcca tgatccgctc ggcccgggcc gcgggggcca gggacccgag ggccgccgtc
     1861 aagagcagga ggcgcgcggc gcgggtcatg gccggaggaa ccggtagagg gaaggcggcg
     1921 tggggagcgg atgggtctgg gggggcgagt tcgtgatcat ggaagaacaa tcgggcccca
     1981 gcagttggat gtcggcgtcc cgctcaaagg cgtccaccca ggtggcgccg ccgtcgtggg
     2041 accaggcttc ccgggacaag tggggcccgc cgtcaaaggt caggtcgcag gcgtcgggca
     2101 ggccgcccga attccccgcc tcggccagca catagcagtc ctcgcagccg ggcgcctgcg
     2161 ggacggcccg gcccgcgtcg gtctggaaga cgatgtagcg gcactcgtcg ggaaatccct
     2221 ggggatggtc cgcggaccga agggggatgg tgatccattg aaaggcgtcg gaggcccggc
     2281 cgggggcgag ggcctcccct tcgaaggcca ggctggattg atggcggcgg tagtcgatgg
     2341 tgtagacccg gtagttcaac ggcgcgtcgg ggcggccttg tcggcggacg cgggcgcgga
     2401 tggccttggc catgaaggcg caatgggggt ggagcgcctc gccgcccagg acatcgtccg
     2461 aggggtccga cggcgtgccg ttgccgtgga tgggaaggga tccgggatcg tcgtagggat
     2521 ccccctgcag ctggtcaccg gcctcatgca gggcgaagag cggttgtcgg tccaaggcct
     2581 tccattttcc cccctcgtac gagagcacgt tcagccttgg atcggcctta ccgtcctgcg
     2641 gatccatccg gttgggccga tccgtgtaac cgaaggcggc ttggttcttc ggcccgatga
     2701 cccccacggg gtggaagcca ccgcgggtca cgttgtgttc gatcacgagg tcgtacacct
     2761 ttccctgggc cagggggatg ttgtccaccg gcagggtgat ccagcgccct gcgaggggga
     2821 ccacactgga gtttcccagg gggacgccgg agggggcccc gtccttatct tcctgcaggc
     2881 tgaccaggta ggcgggtggg tcttgggccg cggtacaata gatggaaagg ccgatgacat
     2941 tggcgtcctg ccggctggtg aagcggaaag ccatcttctg gtccgaggct tggatcacgc
     3001 tggataacag ggaattggcg atgaaagtgg agccatagtc ggcccaaagg gggagggtcg
     3061 atgaccaaag gaggagggtc aggctagggg tgatccatgg gaggcgcatg gtt
//
LOCUS       DASTJJ010000497         1153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_194310, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000497 DASTJJ010000000
VERSION     DASTJJ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194310"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..>1153
                     /locus_tag="VFR02_06145"
     CDS             143..>1153
                     /locus_tag="VFR02_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET9870059.1"
                     /translation="MKKNDPKPKASPKADAAADYHKGMRVEDFVLSFQNNLKYRLIKE
                     PATATDYDRYLSLAYGVRDRLVENWVATQDAYRSKKVKRVYYLSLEFLIGRTLGNSIV
                     NLKVDDTVSRAMEELGYNLEELREVEIDAGLGNGGLGRLAACFIDSLATLEIPAYGYG
                     IRYDYGIFRQKIVHGYQVEEPDEWLAFEHPWEKARPEYCYRVKFGGHVVQRTNRSGKM
                     VFDWVETEDVLAVAYDMPIPGYGNRTVNNLRLWSAKATEEFELDFFNQGDYFAATSKK
                     TATESISKVLYPNDNSPEGRELRLKQQYFFVTASIQDILRRFKREHDDLKRLPEAAAI
                     QLN"
BASE COUNT          230 a          388 c          343 g          192 t
ORIGIN      
        1 gccctgtcct tggcggtggc tttgatccgg tcccttctgc cgtcttcttc cgcaacccct
       61 cccgctttcc aagcaccgcg cccgcgccct tcggtccccg aaaaaggcgt cttggacgtg
      121 gaatacgaga taaaggacga ccatgaaaaa gaacgaccct aagcccaagg cttcgcccaa
      181 ggccgacgcc gcggcggact accacaaggg catgcgcgtg gaggatttcg tcctttcctt
      241 ccagaacaac ctcaagtacc gcctcatcaa ggaaccggcc accgccaccg actacgaccg
      301 ctacttgagc ctggcctacg gcgtgcggga ccgcctggtg gagaactggg tggccaccca
      361 ggacgcctac cggtccaaga aggtcaagcg ggtctattac ctgtccctgg agttcctcat
      421 cgggcgcacc ctgggcaact cgatcgtcaa cctgaaggtg gacgacacgg tgagccgcgc
      481 catggaggag ctgggctaca acctggagga actgcgcgag gtggagatcg acgcggggct
      541 gggcaacggc ggcctgggcc gcctggcggc ctgcttcatc gactcgctgg ccaccctgga
      601 gatcccggcc tacggctacg gcatccggta cgattacggc atcttccgcc agaagatcgt
      661 gcacggctac caggtggagg agcccgacga gtggctggcc ttcgagcatc cctgggaaaa
      721 ggcccgtccc gagtactgct accgggtgaa gttcggcggc cacgtggtgc agcgcaccaa
      781 ccgctccggc aagatggtct tcgattgggt ggagaccgag gacgtgctgg cggtggctta
      841 cgacatgccc atcccgggtt acggcaaccg cacggtcaac aacctgcgcc tttggtcggc
      901 caaggccacc gaggaattcg aactggactt cttcaaccag ggggattatt tcgccgccac
      961 ctccaagaag acggccaccg agtccatctc caaggtgctt tatcccaacg acaacagccc
     1021 cgaggggcgg gaactgcgcc tgaaacagca gtatttcttc gtgacggcct ccatccagga
     1081 catcctgcgc cgcttcaagc gcgagcacga cgacctcaag cgcctgcccg aggccgccgc
     1141 catccagctc aac
//
LOCUS       DASTJJ010000498         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1416139, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000498 DASTJJ010000000
VERSION     DASTJJ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416139"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VFR02_06150"
     CDS             complement(<1..1486)
                     /locus_tag="VFR02_06150"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9870060.1"
                     /translation="MAARRLDAPPWKIALGLGAVLFLLDACLVPSFVGWYDTGELLAA
                     TAALGISHPSGQALYHLLGKCFLAFPFGAPPWRLGLLSAAAGALASALFFLLACRLGE
                     RLAPHPDRPLSPTLRLWLAVLTLAWSLSLPWWTYAVTQLVYSLHLALILGALWIAASR
                     HPRKWQGAALVLGAAVVFRPTQFFGLACLGALYFHETRRRRTGKLRRLAVVASFFLLG
                     WSTALYLPLRSALHPAIAFAALDHPAALLRHLLALKFSPSLGSASPGAMAAILHQMAR
                     RLWEDLTPLGCLLSAWGIFLAWRLRSRLPGFFWAGAAWALLESLLVVSVPYPTFESHQ
                     FLYPWAFLGLTAALSLAWLSHLEVRLDGRGLHRVLAWKKVLPLLLGFWALAQLSRIGV
                     PWDRLHERGAQDFARDVLDSLGRSALYVPSEENQYFPVVGYQLAFGVRPDVEILEPGK
                     DSHDAVGEKLRAALLAGRPVYLNRPVEGLAPGWRFKPEGPLLQIT"
     gene            complement(1488..>2841)
                     /locus_tag="VFR02_06155"
     CDS             complement(1488..>2841)
                     /locus_tag="VFR02_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9870061.1"
                     /translation="AWGGTRSRGAFLALAVCLVLYLFTHYAAVEARLRRFNARQWAVF
                     AGLALFMLACSAFMIDRLLEGEKIDPRAGLRPTLWASAFHMACDQPFFGTGPGTFETV
                     YPYYRSPDLWNTTTPFAHNELLQAAAECGWPACALLLAFTAALFWNSWRLARVNPPFK
                     ALAPPRRAAETALLMLTYVFLQNLVDFTFHAWSILLPLTAWMAFALRDERVGGVEVHL
                     QLTKTARLASAWAVFALAFWVLGIGAWRDAAARLDYYAARGLQAQGSLDQAGKELERS
                     LSLRPNLVEPWSLLGAMAMSRAVTAGDPAVKSAYFRLAETDFEQAVKVSPRSVTPREN
                     LVKLMEVQGRLDQALDLQEDLTGDLPGLPTNYLEQGEILLAQGRPAAVLPLVQKAINL
                     DHYFLEAYFLKAKALEALGRPAQARQVYRDVEAKLRSAGLQDKIPLVENEVRRLKDRP
                     "
BASE COUNT          526 a          958 c         1032 g          325 t
ORIGIN      
        1 gggtgatctg aagcaacggc ccctcgggtt tgaaccgcca gccgggcgcc agcccctcga
       61 ccggacggtt caaatagacg ggacggcccg ccagcagcgc ggcgcgcagt ttttcgccca
      121 ccgcgtcatg gctgtccttc cccggttcca ggatctccac gtcgggcctc acgccgaacg
      181 ccagctggta tcccaccacg ggaaaatatt ggttctcctc ggaaggcaca tagagggccg
      241 accgtcccag gctgtccagc acgtcgcggg cgaaatcctg cgcgccgcgt tcgtgcaggc
      301 ggtcccaggg cacgccgatc cggctgagtt gggccaatgc ccagaacccc aacagcaggg
      361 gcaggacctt cttccaggcc agcacccggt gcaggccgcg gccgtccagg cgcacttcca
      421 gatggctgag ccaggccagg gacagggcgg ccgtcagccc caggaaggcc cagggataga
      481 ggaactggtg cgattcgaag gtggggtaag gcacgctgac caccaacagc gattccaaca
      541 gggcccaggc cgcccccgcc cagaaaaagc ccggcagtcg ggaccgcagg cgccaggcca
      601 ggaagatccc ccaggccgac agcaggcagc ccaggggcgt gaggtcctcc caaaggcgcc
      661 gggccatctg gtggaggatg gccgccatgg ccccgggcga ggccgacccc agggacgggg
      721 agaatttcaa cgccagcagg tggcgcagca gcgccgccgg atggtcgagg gccgcgaagg
      781 cgatggccgg gtgcagcgcc gagcgcaggg gcaggtaaag ggccgtgctc caacccaaga
      841 ggaaaaagga ggcgacgacc gccaggcggc gcagcttccc ggtccgccgg cgccgcgtct
      901 cgtggaagta aagcgccccc aggcaggcca ggccgaagaa ctgggtggga cggaagacca
      961 ccgcggcgcc caggaccagc gcggccccct gccacttgcg cggatggcgg gaagcggcga
     1021 tccacaaggc ccccaggatg agggccaggt gcaggctgta gaccaattgg gtgacggcgt
     1081 aggtccacca gggcaggctc agggaccagg ccagggtcag gaccgccagc cacaggcgca
     1141 gggtgggcga caggggccgg tccggatggg gggccaggcg ctcccccagg cggcaggcca
     1201 ggaggaagaa caaggcggaa gccagggccc ccgccgccgc ggacaaaagg cccaaccgcc
     1261 agggcggggc gccgaaaggg aacgccagga aacacttgcc cagcaggtgg tagagcgcct
     1321 ggcccgaggg atgggagatc cccagcgcgg cggtggcggc caacagttcc cccgtgtcgt
     1381 accagcccac gaaggacggc accaagcagg cgtccaggag gaaaaggacg gcgcccagcc
     1441 ccaaggcgat cttccagggc ggggcgtcca ggcggcgggc cgccaactta aggccggtcc
     1501 ttaagacggc gcacctcgtt ctccaccagg gggatcttgt cctgcaggcc cgccgaccgc
     1561 agcttggcct ccacgtcccg gtagacctgc cgggcctggg cggggcggcc cagcgcctcc
     1621 agggccttgg ccttgaggaa ataggcctcc agaaaatagt ggtccaggtt gatcgccttc
     1681 tgcaccaaag gcagcaccgc ggcgggcctg ccttgggcca acaggatctc gccctgctcc
     1741 aggtagttgg tgggcaggcc cggcaggtcg ccggtcaggt cctcctgcag gtccagggcc
     1801 tggtcgagcc ttccctgcac ctccatgagc ttgaccaggt tctcccgggg cgtgacggac
     1861 ctaggcgaca ccttgaccgc ctgctcgaaa tcggtctccg ccagccggaa gtaggcggac
     1921 ttgaccgccg gatccccggc ggtgacggcc cgggacatgg ccatggcccc caacagcgac
     1981 cagggttcca ccaggttggg ccgcagggac aggctgcgct ccagttcttt cccggcctga
     2041 tccagcgagc cctgcgcctg cagcccccgc gccgcgtaat agtccaggcg cgcggcggcg
     2101 tcccgccagg ccccgatgcc cagcacccag aaggccaggg cgaagaccgc ccaggccgac
     2161 gccaaccggg cggtcttggt gagctgcagg tggacctcca cgccgcccac ccgctcgtcg
     2221 cgcagggcga aggccatcca agcggtgagc ggcagcagga tggaccaggc gtggaaggtg
     2281 aaatccacca ggttctgcag gaagacgtag gtcaacatga gcagggccgt ttcggccgcc
     2341 cggcggggcg gggccagggc cttgaagggc gggttcaccc gcgccagccg ccagctgttc
     2401 cagaacaggg ccgccgtgaa ggccagcagc agggcgcagg cgggccagcc gcattcggcg
     2461 gcggcctgca ggagctcgtt gtgggcgaag ggggtggtgg tgttccagag gtcgggggaa
     2521 cggtaatagg gatagaccgt ctcaaaggtg ccagggcccg tcccgaagaa gggttggtcg
     2581 caggccatgt ggaaggcgct ggcccaaagg gtggggcgca ggccggcgcg cgggtcgatc
     2641 ttttccccct ccagcagccg gtcgatcatg aaggccgagc aggccagcat gaagagcgcc
     2701 aggccggcga agaccgccca ctgacgggcg ttgaaccggc gcaggcgggc ctccacggcg
     2761 gcatagtggg tgaagaggta cagcaccagg cagaccgcca gcgccaggaa ggcgccccgg
     2821 ctgcgggtgc ccccccaggc c
//
LOCUS       DASTJJ010000499         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1661926, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000499 DASTJJ010000000
VERSION     DASTJJ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661926"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFR02_06160"
     CDS             complement(<1..760)
                     /locus_tag="VFR02_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870062.1"
                     /translation="MALLALDVLYVRSLFYFEIQDTDINCYAGIAHGLLQGRALYSDL
                     WDFKPPAVFWTYALAEKICGYGLSEVFFLNILFNGLALAGAYACGYGLSRSRWTGFLA
                     ACFWALVSPQVPLGANEPNTELFINVLQLFALLFWAGWKEGGGRRIFPLTAGLCLGAA
                     ALYKQVPVITACCMALAPLLAAKGTAARRAAAGEAGRLAAGFLAPWALTCAYYWAGGR
                     LGLFLQATFADNLDQVSLADNLWSGWMPKNLWPGF"
     gene            complement(838..>1139)
                     /locus_tag="VFR02_06165"
     CDS             complement(838..>1139)
                     /locus_tag="VFR02_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HET9870063.1"
                     /translation="RYARPKREVLEAVHRVLAGLRGWRIEEYRENQGRVHAVHFGPFP
                     LPFQDVNFYVIQGLDGNSKLEATSQSRGGGGDWGQNRRNLRRFLDRLDALLTPLA"
BASE COUNT          225 a          388 c          379 g          147 t
ORIGIN      
        1 aaaagccggg ccacaggttt ttcggcatcc aacccgacca caggttgtcc gccagggaaa
       61 cctggtccag gttgtcggcg aaggtcgcct gcaggaacaa ccccagccgg cctcccgccc
      121 agtaataggc gcaggtcaag gcccagggcg ccaggaaacc agcggccagg cgtcccgctt
      181 cccccgcggc cgcgcgccgg gcagccgtcc ccttcgcggc cagcaacggc gccagggcca
      241 tgcagcaagc ggtgatgacc gggacctgct tgtaaagggc cgccgcgccc aggcaaagtc
      301 ccgccgtcag ggggaatatc ctccggcccc ccccttcctt ccaaccggcc caaaagagga
      361 gggcgaaaag ctggaggacg ttgatgaaca actcggtgtt gggctcgttg gcccccaggg
      421 ggacctgggg cgagaccagg gcccagaagc aggccgccag gaacccggtc caccgggaac
      481 gggacaagcc gtaaccgcag gcataggcgc ccgccagggc caggccgttg aagaggatgt
      541 tcaggaagaa aacttccgag aggccgtaac cgcatatctt ttccgcgagg gcgtaggtcc
      601 agaaaaccgc cggcggcttg aaatcccaca ggtccgaata gagcgcgcgg ccctgcaaca
      661 ggccatgggc gatccccgcg tagcaattga tgtccgtgtc ttggatctcg aaataaaaga
      721 gggaccggac gtagaggaca tccaacgcca gcagtgccaa gcccaaaccc aaccacccac
      781 gcgggccccg gtccggatcg cgcgcggggc ccgtcatttt gcggggacgg ggcaaggtca
      841 ggccaggggc gtcagcaggg cgtccaggcg gtccaggaag cgccgcaggt tgcgccggtt
      901 ctggccccaa tcgcccccgc cgccgcgcga ctgcgaggtg gcttccaact tcgagttgcc
      961 gtccaggccc tggatcacgt agaagttcac gtcctggaag ggcaggggga aggggccgaa
     1021 atggaccgcg tggacgcgcc cctggttctc ccggtattcc tcaatgcgcc agcccctgag
     1081 gcccgccagc acccggtgga cggcctccag cacctcccgc ttggggcgcg cgtagcggc
//
LOCUS       DASTJJ010000500         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1171794, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000500 DASTJJ010000000
VERSION     DASTJJ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171794"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFR02_06170"
     CDS             <1..747
                     /locus_tag="VFR02_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9870064.1"
                     /translation="TSIQQITRDNRQRAIQFTAGTGPGVSQQEAVQEALAICKSVLPP
                     GYSAQLTGTAQANKDSLKQLGFVMLLGIIVAYMVLGSQFNSFVHPVVILLALPFSITG
                     ALFSLWLTGQSLSTYSMIGMILLLGLVKKNSIMLVDFTNQRRKLDGMNVTEALLAACP
                     VRLRPILMTSAATIAGAIPAAVAVGPGAELRQGMSIAIIGGMFFSTLLTLLVVPCAYS
                     LMSHLESSRPHDFEWDEAGNLTTGRKARKR"
     gene            complement(846..>1084)
                     /locus_tag="VFR02_06175"
     CDS             complement(846..>1084)
                     /locus_tag="VFR02_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870065.1"
                     /translation="LQLKSGGLQNRMPAQSHEATKKARKREIYFMDLSNFSFFCRSWR
                     TWRLGGEQTFVTGLIFPGFTPGGEVSLPGPKGHY"
BASE COUNT          215 a          357 c          305 g          207 t
ORIGIN      
        1 acctccatcc agcagatcac ccgcgacaac cgccagcggg ccatccagtt caccgcgggc
       61 acgggcccgg gcgtgtccca gcaggaggcg gtccaggagg ccctggccat ctgcaagagc
      121 gtgctgcccc cgggctacag cgcccaattg accggcacgg cccaggccaa caaggactcg
      181 ctgaaacaac tgggcttcgt catgctgctg gggatcatcg tggcctacat ggtgctgggc
      241 agccaattca acagcttcgt gcaccccgtg gtcatcctgc tggcgctccc cttcagcatc
      301 acgggcgcgc tcttctcgct gtggctgacc gggcaatccc tgagcaccta cagcatgatc
      361 ggcatgatcc tgctgttggg gctggtgaag aagaactcca tcatgctggt ggacttcacc
      421 aaccagcgcc gcaagctgga cggcatgaac gtgaccgagg cgctgttggc cgcctgcccg
      481 gtgcgcctgc ggcccatctt gatgacctcc gcggccacca tcgccggcgc catcccggcg
      541 gcggtggcgg tgggtccggg cgccgaactg cgccagggca tgtcgatcgc catcatcggg
      601 gggatgttct tctcgaccct tttgaccctc ttagtggtcc cctgcgccta cagcctcatg
      661 tcccacctcg aaagctcccg cccccacgat ttcgagtggg acgaagcggg caacttgacc
      721 actgggcgga aagcccgaaa acgttagttg gtttgagcgt caggcgaatc ccaaccccat
      781 cgcttcgcgt tgaaaggctc ctcggaacgc cttccaccaa ggcgatcctt tccacgcctt
      841 ggtggtcaat aatgaccttt cggcccaggg agggaaacct caccaccggg cgtaaagcca
      901 ggaaaaataa gacctgtcac aaaagtctgt tcaccgccaa gacgccaagt acgccaagaa
      961 cgacaaaaga atgaaaagtt cgaaagatcc ataaaataga tctccctttt tcgggctttc
     1021 tttgtggctt cgtggctttg tgcgggcatt cggttttgca gccccccact cttcagttga
     1081 agcc
//
LOCUS       DASTJJ010000501         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_71536, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000501 DASTJJ010000000
VERSION     DASTJJ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71536"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..516
                     /gene="msrB"
                     /locus_tag="VFR02_06180"
     CDS             10..516
                     /gene="msrB"
                     /locus_tag="VFR02_06180"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9870066.1"
                     /translation="MIILCLAGGAAFPAAAGPGPAVTKPGGPYMGDKIVKTDAEWKKE
                     LTPEQYDVLRHQGTERAFTGPYWDNHQKGLYVCAGCGLALFTSATKFDSGTGWPSFTQ
                     PVYTQNVETREDDSHFMKRVEVVCPRCGGHLGHVFDDGPAPTGLRYCINSAALKFEPA
                     DPDKNAKK"
     gene            594..>1831
                     /locus_tag="VFR02_06185"
     CDS             594..>1831
                     /locus_tag="VFR02_06185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9870067.1"
                     /translation="MRDWAKPATTLFLALGLGGACFCVDLTHLAVNHTYDGMVFASLI
                     EQRHPSAWDLFHPHHLIYNWLGRQFFLFGHTHGADWDGLGALQCFDLLTGTAGVLLVF
                     HLLVRVTGDRGTAFLSALGLSFTYSYWYFSTSPGVRIFATVTPLLAWYAFTYVKDRGV
                     GWGAVLGLAHAFAVLGHQTNLLLLPAFLGGIWCVREKTFREKIGISLLYLAVLTAAVL
                     GAYGFVGRFLCERTTYSAWVWWIMSYMHVKEWGGHLGPAGFERGKFAMVFAFLAGAYP
                     TKPLGDYLTFGFAKGIFQNALLFVLGALLLRARAFWARYPQTLWIGFFWLMAFVPFFV
                     WWEPWNIEFWVSSTVPCWILMGTVVSDLSLKFTNPVLRFANRLLFLFLWGCLIGLLFL
                     YNFEGRGVHATPYANKTLMSA"
BASE COUNT          263 a          611 c          571 g          386 t
ORIGIN      
        1 cgaaagacct tgatcatttt atgcttggcc gggggcgcgg cctttcccgc cgccgccggt
       61 cccgggccgg ccgtcaccaa accaggaggg ccttatatgg gggacaagat cgtcaagacc
      121 gacgccgagt ggaaaaaaga actgacgccc gagcaatatg acgtgctgcg ccaccagggc
      181 accgagcggg ccttcacggg cccctattgg gacaaccacc aaaagggcct ctatgtctgc
      241 gcgggctgcg ggctggcgct cttcacctcc gccaccaagt tcgattcggg gacgggctgg
      301 cccagcttca cccagccggt ctatacccag aacgtggaga cccgggaaga cgattcccat
      361 ttcatgaagc gcgtggaggt ggtctgcccc cgctgcgggg ggcacctggg ccacgtgttc
      421 gacgacggcc ccgcccccac ggggttgcgc tactgcatca actccgccgc gctgaagttc
      481 gagcccgccg acccggacaa gaacgccaag aagtaggcgc gcgcctcggg gtgacaagac
      541 cgcctttttt gctatgatgg ccccgcttct cactcgggat ccgaaggggt gtcatgcggg
      601 attgggcgaa accggccacg accttgttcc tggccttggg tctggggggc gcctgcttct
      661 gcgtcgacct cacccacctg gcggtcaacc acacctacga cgggatggtc ttcgcctccc
      721 tcattgagca gcggcacccc tccgcgtggg acctgttcca tccccatcac ctgatctaca
      781 actggctggg ccggcagttc ttcctcttcg gccacaccca tggcgccgat tgggacggcc
      841 tgggggcgct ccagtgtttc gacctgttga ccgggacggc cggggtgttg ctggtcttcc
      901 acctgttggt ccgggtgacg ggggaccggg gcacggcctt cctgtccgcc ttgggcctct
      961 ccttcaccta tagttactgg tatttctcca cctcgccggg ggtgcgcatc ttcgccaccg
     1021 tcacccccct gctggcctgg tacgccttca cctacgtgaa ggaccggggc gtcggttggg
     1081 gcgccgtgct gggcctggcc catgccttcg ccgtgctggg ccaccagacc aacctgctgc
     1141 tgctgccggc cttcctgggc ggcatctggt gcgtgcgcga aaagaccttc cgggaaaaga
     1201 tcgggatcag cctgttgtac ctggcggtcc tgaccgcggc cgtgttgggg gcctacgggt
     1261 tcgtggggcg gttcctttgc gagcgcacca cctattcggc ctgggtctgg tggatcatgt
     1321 cctacatgca cgtgaaggaa tggggcggcc acctgggccc ggcgggtttt gagcggggca
     1381 agttcgccat ggtcttcgcc ttcctggcgg gggcctatcc caccaagccg ctgggggact
     1441 acctgacctt cggcttcgcc aagggcatct tccagaacgc cctcctgttc gtgctgggcg
     1501 ccctattgct gcgcgcccgg gccttttggg cccgctatcc gcagacactc tggatcggct
     1561 ttttctggct gatggccttc gtgcctttct tcgtctggtg ggaaccctgg aacatcgagt
     1621 tttgggtctc ctcgaccgtg ccctgttgga tcctgatggg cacggtggtc tccgacctat
     1681 ccttgaaatt caccaatccg gtcttgcggt tcgccaaccg gttgcttttc cttttcctct
     1741 ggggctgcct gatcggcctg ctgttccttt acaacttcga gggccggggc gtccacgcga
     1801 ccccctacgc caacaagacc ctgatgtcgg c
//
LOCUS       DASTJJ010000502         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_928673, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000502 DASTJJ010000000
VERSION     DASTJJ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928673"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VFR02_06190"
     CDS             complement(<1..1321)
                     /locus_tag="VFR02_06190"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET9870068.1"
                     /translation="MPRRRPAAWPFLLFCLSALAAPAAFSQVLTVGDSPQPLVMDGHL
                     KDWPASRMITLDDPGQVVEGGFFWKGPDQFGARVFLTYDAEYLYLAVLVTREGPPVNS
                     NKPSALWNGDCLELFLSTDPGFASQGRLTRADYHVGISPGTDCQDPQAWCFNLEEAIP
                     GSQVTAKISKKGYLMEAALPLDFFRGLQIAPGGTCGFDLALDEGGPVGGNRLARLDYL
                     GHRETWLHPAGWAPLKWTGATQVTIPPPDESDENAILVRDGTRGAGFLGKKSFTGRVV
                     DGQGRPLAGAEVSLWPADAVTVTDGSGSFALGPVKAYRKTLLYARHDGYFTSLAAPQA
                     AGRPVTLCLSSMPVTAPGPQHARSPLFFGAGFSLDAAGRIPADLPVSLVRGLGLGVLY
                     LRGGCLAGLDDEAQKGALVRFQDYARALGAEPVVEVPLGPDAASRAVS"
BASE COUNT          271 a          500 c          453 g          201 t
ORIGIN      
        1 aggaaaccgc ccgggacgcc gcgtcggggc ccaaggggac ttccaccacg ggctcggcgc
       61 caagggcccg ggcgtaatcc tggaaccgca ccaaggcccc cttttgggct tcgtcatcca
      121 accccgccag gcacccgccc cgcaggtaca aaacccccaa ccccaaaccg cgcaccaggc
      181 tgacgggcag gtcggcgggg atccgcccgg cggcatccag cgaaaacccc gccccgaaga
      241 acagcgggct gcgggcatgc tggggaccgg gcgccgtcac gggcattgag gacaaacaca
      301 gggtgaccgg ccgtccggcg gcctgggggg ccgccaggct ggtgaaataa ccgtcgtggc
      361 gcgcgtacaa caaggtcttc cggtaggcct tgaccgggcc caaggcgaag ctcccggacc
      421 cgtccgtgac cgtcacggca tccgcgggcc aaaggctcac ctcggcgccc gccaaaggac
      481 ggccttggcc gtccaccacc cgccccgtga agctcttctt gcccaggaac ccggccccgc
      541 gggtcccgtc gcggaccaag atcgcgttct catcgctttc atcgggcggg gggatcgtca
      601 cctgggtggc cccggtccat ttcaagggcg cccatccggc cggatgcagc caagtttcgc
      661 ggtgtcccaa ataatccagc cgggccagcc ggttcccccc caccggcccc ccttcgtcca
      721 aggccaagtc gaatccacag gtcccgcccg gggcgatctg caggccgcgg aagaaatcca
      781 gaggaagggc ggcttccatc aggtagccct tcttcgagat cttggcggtg acttgggagc
      841 ccgggatggc ctcttccagg ttgaaacacc aggcctgggg atcttggcag tccgtccccg
      901 gggaaatgcc cacatgataa tccgcccggg tgagccgccc ttgggaggcg aaacccgggt
      961 cggtggagag gaatagttcc aggcaatccc cgttccaaag ggccgaaggc ttgttcgaat
     1021 tgaccggggg gccctcccgc gtcaccagga cggccagata aagatattcg gcgtcatagg
     1081 tgagaaagac ccgggcgccg aattgatccg ggcccttcca aaagaaccca ccttccacca
     1141 cctggccggg atcgtccagg gtgatcatcc ggctggcggg ccaatccttc aaatggccgt
     1201 ccatgaccaa gggttggggg ctgtcgccga ccgtcaggac ttgggaaaat gcggccgggg
     1261 cggccaaggc cgaaaggcaa aagagcagga acggccatgc cgcgggccgg cgtctcggca
     1321 cgatagctcc ccccttcgcc aaggtttggg accggtggcg acccaggcct acagcaccga
     1381 ctccaacagg gtctccccca tcaaccactt atccacgcct tgggc
//
LOCUS       DASTJJ010000503         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_803848, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000503 DASTJJ010000000
VERSION     DASTJJ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803848"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFR02_06195"
     CDS             complement(<1..742)
                     /locus_tag="VFR02_06195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9870069.1"
                     /translation="MRHPLLRLLKGAAKAVGGHGLGRVRPIRWAYDRLYQALKPQWVR
                     VQGHRMFLDEKDTLELATREVYEPLETRLLLEHLAPGQVFVDIGANIGYYTLLAARRV
                     GPEGRVFAFEPDPDNFRLLQKNVAVNGYANVILEPKALSDGEGEADLFLNPLNRGDHR
                     LYDSGDGRVHVRVPRTALGSYFKAVQNRPHFIKMDIQGAEARALAGMRDLVAGLPRVL
                     LVSEFGPEGLRRSGADPAGYLRDLASLGF"
     gene            complement(742..>2425)
                     /locus_tag="VFR02_06200"
     CDS             complement(742..>2425)
                     /locus_tag="VFR02_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870070.1"
                     /translation="LTFAMLFLGGSFQVSIGAFYAGAAYLALRAFQNRGAFVRTAGRG
                     GFWLSFAAGAAPLAAQLVPTAEFVLRSARHGWTGDYWKYNALPSLEPHSLPHLLFPGL
                     DLPPGQLMDHVLQNNPVFLSNELYLGVLAPLLVALAFRQPRQAVHWFFLALGATALGL
                     CLGGHFFLHRWVVDHVPGFAGLRSPFRFSYLWTFSFATLAAWGWQTLESLGPSPKLPR
                     RWIVLFALYAAFLSALALPHPRWTDLELGGLGLASLGLVLVFRRRRAWGLACLETALL
                     LPLLLRGWQGYEPAPIVNYDFKRNSAALIRLVAPQMPGRVCINPLQIPYPIRAHGLDY
                     VSYYPENVFAALKLKDLGGYNPLSLQAVDDLRYLPPDKTFKLMAVRGFLVGMDVGPLA
                     GFQRSGRDHFYFYRSLFSGDLVFCPRRWEVLADGGARLRAMAQPAFDPYQEGVLASPL
                     PAEQAARLTGKPADLRYALERDGAEDWTYRVQLRRDGLVVFSQPAYPGWRARVDGTAA
                     PLLTADHALQALFLPAGTHRVEFTYDPWWKPWLPLPWLACLLAGLWLYRRKP"
BASE COUNT          469 a          756 c          891 g          309 t
ORIGIN      
        1 ggaaacccag ggaggccagg tcccgcaggt acccggcggg gtcggcgccg ctgcggcgca
       61 ggccctcggg gccgaattcg ctcaccagca gcacccgggg caggcccgcc acgaggtccc
      121 gcatccccgc caaggcccgg gcctcggcgc cttggatgtc cattttaatg aaatggggcc
      181 ggttttgcac cgctttgaaa taggacccca gcgcggtccg ggggacccgg acgtggacgc
      241 ggccgtcccc cgagtcgtag agccggtggt cgccccggtt gagggggttc aggaaaaggt
      301 cggcttcgcc ctcgccgtcc gagagggcct tgggttccag gatgacgttg gcgtagccgt
      361 tcaccgccac gttcttttgg agcaggcgga aattgtccgg gtcgggctcg aaggcgaaga
      421 cccggccttc cgggcccacc cgccgggccg ccagcagggt gtagtagccg atgttggccc
      481 cgatgtcgac gaagacctgg ccgggcgcca ggtgttccaa caagaggcgc gtctccaggg
      541 gctcatagac ctcccgggtg gccagttcca gggtgtcctt ctcatccagg aacatccggt
      601 ggccctggac ccggacccat tggggtttga gggcctggta gaggcggtca taggcccagc
      661 ggatgggccg gacgcggccc aagccgtggc cgccgacggc cttggcggcc cccttcaaga
      721 gccgtaaaag cgggtggcgc atcagggttt ccgccggtaa agccaaaggc ccgccagcag
      781 gcaggccagc cagggaaggg gcagccaggg tttccaccag ggatcgtagg tgaactccac
      841 gcggtgggtc ccggcgggca ggaagagggc ctgcagggcg tggtcggcgg tgaggagggg
      901 ggcggctgtg ccgtccaccc gggcccgcca gcccggatag gccggttggg aaaagaccac
      961 caggccgtcg cggcgcagtt ggacccggta ggtccaatcc tccgccccat cccgctcgag
     1021 ggcatagcgc aggtcggcgg gcttgcccgt gaggcgggcg gcctgctcgg ccggcaaggg
     1081 cgaggcgagg acgccttcct ggtagggatc aaaggcgggt tgggccatgg ccctcaagcg
     1141 ggcccccccg tcggccagca cttcccaacg ccggggacaa aagaccaggt cgcccgagaa
     1201 gaggctgcga tagaaataaa aatggtcccg acccgatcgt tggaacccgg ccaggggccc
     1261 cacatccatg ccgaccagga aaccccggac ggccatgagc ttgaaggtct tgtcgggggg
     1321 caggtaccgc aggtcgtcga cggcctgcag gctcaagggg ttgtagccgc ccaggtcctt
     1381 cagcttgagg gccgcgaaaa cgttctccgg gtaataagag acatagtcca ggccgtgggc
     1441 ccggatggga taggggatct gaagcgggtt gatgcagacc cggccgggca tctggggggc
     1501 gaccaaccgg atgagcgcgg ccgagttgcg cttgaagtcg tagttgacga tgggggccgg
     1561 ttcatagccc tgccagccgc gcaacaagag gggaagcaac agggcggttt ccaggcaggc
     1621 cagtccccag gcccggcggc ggcggaagac caagaccagc ccgaggctgg ccaaccccaa
     1681 cccgcccaat tccaagtcgg tccatcgggg atggggcaag gccaaggccg acaagaaggc
     1741 ggcgtaaagc gcgaaaagga cgatccaacg cctggggagc ttgggggacg ggcccagcga
     1801 ctccagggtt tgccagcccc aggccgccag ggtggcgaaa ctgaaggtcc aaagatagga
     1861 aaaacggaac ggggaccgca acccggcgaa accagggaca tggtccacca cccagcgatg
     1921 caggaaaaag tgcccgccca ggcacaggcc cagggccgtc gcgcccaagg ccagaaaaaa
     1981 ccagtggacc gcctgacggg gctggcggaa tgccagggcc accaggaggg gggccaggac
     2041 gcccaggtag agttcgttgc tcaggaagac ggggttgttc tggaggacat ggtccatcag
     2101 ttggcccggc gggaggtcca ggcccgggaa gagcaggtgc gggagggaat ggggctccag
     2161 ggagggcagg gcgttgtatt tccagtaatc accggtccaa ccgtgccggg ccgaacgcag
     2221 gacgaattcg gccgtcggga ccagttgggc ggccaggggc gccgccccgg cggcgaacga
     2281 aagccagaac cctccccggc ccgcggtccg gacgaaggcg ccgcggtttt ggaacgcccg
     2341 caacgccaga taggcggccc ccgcgtagaa ggccccgatg gacacctgga aactcccccc
     2401 caggaaaagc atggcgaaag tcaag
//
LOCUS       DASTJJ010000504         1310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1783517, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000504 DASTJJ010000000
VERSION     DASTJJ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783517"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFR02_06205"
     CDS             <1..1197
                     /locus_tag="VFR02_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET9870071.1"
                     /translation="RRGLVHFEGWATVPQYETWMAGIGSAANPRQHTDLNTDPGTSAG
                     PGADGVRTDGETPGTGANPASGNDGSLESSRDGDDVMPGQMLLDGLLAPSEEPGVQDS
                     RDSQDSQDSRGNRDGFGGEGGENPGGAATLWPPAGQGPAPDWAQEPGSATGSATNADY
                     ETAAPWPHLVNGIQVPEPGSDEELPELDPIDPACTNPVVADRRTRAQKLLDGLIDCIK
                     LAARTGKLPMNGGLKPQLFISTNETELQRHTKDGRPGGIAFLPYSGPQPLTLLEEELC
                     DADITTMILGNGDNILNVGRTQRFFTDTQRKILIARDKGCCFPHCRRTAMTTDAHHII
                     PWSQGGGTDISNGALLCTVHHHAVHQGLWSIELINGTPWFTPCYKLDPTQTKLRNHYH
                     HGLPGT"
BASE COUNT          300 a          540 c          336 g          134 t
ORIGIN      
        1 cgccgcggcc tggtccactt cgaaggctgg gccaccgtgc cccaatacga gacctggatg
       61 gccggcatcg gatccgcagc caacccccgc caacacaccg acctcaacac cgaccccggc
      121 accagcgccg gtcccggcgc cgatggagtc aggacggacg gcgagacacc gggcaccggc
      181 gcgaaccccg catccggcaa cgacggcagc ctcgaatcca gccgggacgg cgatgatgtg
      241 atgcccgggc aaatgctcct ggacggcctc ctcgccccca gcgaagaacc tggagtccag
      301 gacagccggg acagtcagga cagccaggac agccggggca accgggacgg attcggcggg
      361 gaaggcgggg aaaacccggg cggcgcagcc accctgtggc ccccggcagg ccagggcccg
      421 gccccggact gggcccagga acccggcagt gcaaccggca gtgcaaccaa cgcggactat
      481 gaaaccgcgg cgccgtggcc acacctggtc aacggcatcc aggtgcccga gcccggctcc
      541 gatgaggaac ttcccgaact ggacccgatc gaccccgcct gcaccaaccc cgtcgtggct
      601 gataggcgca cccgcgccca aaaactcctc gacggcctga tcgactgcat caaactcgcc
      661 gcccgcaccg gcaaactccc catgaacggc ggactcaaac cccaactgtt catctccacc
      721 aacgaaaccg aactccaacg ccacaccaag gacggccgcc ccggaggcat cgccttcctc
      781 ccctacagcg gaccccaacc cctgaccctc ctcgaggaag aactctgcga cgcagacatc
      841 accaccatga tcctgggcaa cggagacaac atcctcaacg tcggccgcac ccaacgcttc
      901 ttcaccgaca cccaacgcaa aatcctcatc gcccgcgaca aaggctgctg cttcccccac
      961 tgccgacgaa ccgccatgac caccgacgcc caccacataa tcccatggtc ccaaggcggc
     1021 ggaaccgaca tctcaaacgg cgccctgctc tgcacagtcc accaccacgc cgtccaccaa
     1081 ggcctgtgga gcatcgaact catcaacggg accccctggt tcacaccctg ctacaaactc
     1141 gaccccaccc aaaccaaact ccgaaaccac taccaccacg gcctccccgg cacctgaccg
     1201 accacccgat cattgaaccc gcccccgctg ataaggcccg ccgaaaccaa gcccacgaca
     1261 agcccgaccg acgccggaca ccagaccgac ccgcggcgtc cagccgagtc
//
LOCUS       DASTJJ010000505         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1662519, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000505 DASTJJ010000000
VERSION     DASTJJ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662519"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..2103
                     /gene="asnB"
                     /locus_tag="VFR02_06210"
     CDS             187..2103
                     /gene="asnB"
                     /locus_tag="VFR02_06210"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9870072.1"
                     /translation="MCGIAGFTQFKAGLAYQSRDLDAMVAAVKHRGPDDTGTYFNRFT
                     GLGMSRLAIVDLAGGAQPVCNERGTVWVVFNGEIYNHQELRGPLEAAGHRFRSRSDTE
                     VLVHAYEEYGDAFVTRLRGMFAFALWDETFQKLLLARDHLGVKPLYYTTAQGSLLFAS
                     EPKALLEAPGVEAKADHRQILSLMTLQYVPSPDTLLKGVRKLPPGHTLTCQSGKVTLK
                     PYWRLPLPTEPMPEKVTAAMEREWVERLRYQFFLSVKEQLMADVPLGAFLSGGLDSSF
                     VVASMTHQTRQRVRTYSVGFDNQKDFNELKHAQRVSAYLKSQHREIMVDARMLQDLIP
                     RLVKYQDDPVLDPAVLPTFVVSLFARQEVKAVLTGEGADEMFGGYRRYAYDRLSGGVR
                     SLPGWLKDRLLPALTGRLGARTGQALDALRKEDLLKRHLAWSRLCREGTLESLAGEGL
                     RYEMEHNRPEEVFERLYEEAQPYGLDPLNLTLYLDLHTWLPDDLLNKVDRMSMAASLE
                     ARVPYLDHRLVEFAFSLPSSMKLKGGVGKYLLKRAAQKYLPREIIHRRKQGFGVPLGP
                     WFRKELKPLLVDNLTSERFKRRGLFDLGTTNRLLAEHLEGREDHHLLLYGMLMVELWH
                     QRFIDRSLQDPPGS"
     gene            2325..2882
                     /gene="efp"
                     /locus_tag="VFR02_06215"
     CDS             2325..2882
                     /gene="efp"
                     /locus_tag="VFR02_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9870073.1"
                     /translation="MISVNDLRAGNLCEFEGVLYRVTSYQHVKPGKGGGFVRLKLKNV
                     ELGTVTDKTLDSWEKVPEPPVEEKEMQYLYKQGDKFVFMDTETYEQLELTEEEVGEGA
                     QWLKEETSIEILFYKERPISVKLPITVNLKVVSSEPGIKGDRVSGASKPAVLESGATL
                     QVPLFIKEGDVLKIDTRTGEYIERV"
BASE COUNT          572 a          906 c          921 g          566 t
ORIGIN      
        1 tgttgttgaa aaaccaccgt tgggccgaca tcggccgccg gttggaaagg atccttctgg
       61 aggcggcctc tcgtccggcg gacggtaagg ccaagccctg agggcggatc cgtcatttgt
      121 cgtgggccgt cctggggccc ctgtggtgaa atagggttgc cttgatcctc gttcaaaagg
      181 tccatcatgt gcggcatcgc gggtttcacc caatttaagg caggcctggc ctatcaaagc
      241 cgggacctgg acgccatggt cgcggccgtc aaacaccggg gacccgacga caccggcacc
      301 tacttcaacc ggttcacggg gttgggcatg agccgcctgg ccatcgtgga cctggcgggc
      361 ggggcccagc cggtctgcaa cgagcggggg accgtctggg tggtcttcaa cggggagatc
      421 tacaaccacc aggaattgcg cggtccgttg gaggcggcgg gccatcggtt ccgcagccgc
      481 tcggacaccg aggtgttggt gcacgcctac gaggaatacg gcgacgcctt cgtcacccgc
      541 ctgcggggca tgttcgcctt cgccctttgg gacgagacct tccaaaaact gctgttggcc
      601 cgggaccacc tgggggtgaa gcccctctat tacaccacgg cccaggggtc gttgcttttc
      661 gcctccgagc ccaaggcctt gttggaggcg ccgggcgtgg aggcgaaggc cgaccaccgc
      721 cagatcctgt ccctgatgac gctgcagtac gtcccctcgc ccgacacgct gctcaagggc
      781 gtgcgcaaac ttcccccggg ccacacgctc acctgccaga gcggcaaggt gaccctcaaa
      841 ccctattggc gcttgcccct gcccaccgaa cccatgcccg aaaaggtgac cgccgcgatg
      901 gaacgcgaat gggtggaacg gttgcgctat cagttcttcc tgagcgtgaa ggaacagctg
      961 atggccgacg tgcccctggg ggcttttctg tccggcgggt tggactcttc cttcgtggtg
     1021 gcctccatga cccaccagac ccggcaacgg gtgcggacct attcggtggg gttcgacaac
     1081 cagaaggact tcaacgagct caagcacgcc cagcgggtgt cggcctacct gaaaagccag
     1141 caccgggaga tcatggtgga cgcccgtatg ttgcaggacc tgatcccccg gctggtcaag
     1201 taccaggatg atccggtgct ggacccggcc gtcctgccca ccttcgtggt gtcgcttttc
     1261 gcccggcagg aggtcaaagc ggtcctcacc ggcgagggcg ccgacgaaat gttcgggggc
     1321 taccgccgtt acgcctacga ccgcctgtcg ggcggggtgc ggtccctgcc cgggtggctc
     1381 aaggaccggt tgctgcccgc gctcacgggg cggttggggg cgcgcacggg ccaggccctg
     1441 gacgcgctgc gcaaggagga cctgttgaag cggcacttgg cctggtcccg attgtgccgg
     1501 gagggcaccc tggagtccct ggcgggcgag ggtctccggt acgaaatgga acacaaccgg
     1561 ccggaggagg tcttcgagcg cctttacgag gaagcccagc cctacggcct ggatcccctc
     1621 aacctgaccc tgtatctgga cctgcacacc tggcttcccg acgacctgct caacaaggtg
     1681 gaccgcatga gcatggccgc ctccctggag gcccgggtgc cctacctgga ccaccgcttg
     1741 gtcgaattcg ccttctcctt gccttcctcc atgaagctca agggaggggt ggggaagtac
     1801 ctgctcaaac gcgcggccca aaagtacctg ccccgggaga tcatccaccg ccgcaagcaa
     1861 ggcttcgggg tccccctggg cccctggttc cgcaaggaac tgaaacccct gttggtggac
     1921 aacctgacct ccgaacgctt caaacgcagg ggcctttttg acctggggac cacgaaccgg
     1981 ttgctggccg agcacctgga aggccgggaa gaccatcacc tccttcttta tgggatgttg
     2041 atggtggaac tttggcacca gcggttcatc gaccgctccc tacaggatcc gcccgggtct
     2101 tgaccctccc tttgcccctg tctttcattt gatcttttcc ggcatttgaa aaaacccttt
     2161 atttccaggc gtttttaaag accctccctg gccccgtccg cccatgaatt gacggatggg
     2221 tgtctatcga gtaaaatgcc cctccttttt gtggtttttg agttttgaca taaaacttga
     2281 caatttattg gccttttttc gccatttcct aaggagcttc ttgaatgatc agcgtgaacg
     2341 accttcgggc cgggaatttg tgtgagttcg agggggtcct ctaccgggtg acgtcctacc
     2401 agcacgtgaa accgggcaag ggcggcggct tcgttcgctt gaaactcaag aacgtggagt
     2461 tggggacggt caccgacaag accctggaca gctgggaaaa ggtccccgag cccccggtgg
     2521 aagaaaagga aatgcagtac ctctataaac aaggcgataa gttcgttttc atggataccg
     2581 agacctatga acagttggag ttgaccgagg aagaggtggg ggagggggcc caatggctga
     2641 aagaggagac ctccatcgag atcctctttt ataaggaacg gccgatcagc gtgaagctgc
     2701 ccatcacggt caatttgaag gtggtctctt ccgagccggg catcaagggc gaccgggtct
     2761 cgggcgccag caagccggcc gtcctggaaa gcggggcgac ccttcaggtg cccctgttca
     2821 ttaaggaagg cgacgtgctg aaaatcgaca cccgtaccgg cgagtatatc gagcgggttt
     2881 aaccccatcc gaagcgcgag cctaaaggag gcaccatgac gacccctgag aagaacgaga
     2941 agcccgaaaa gaaaaagaaa gcccc
//
LOCUS       DASTJJ010000506         1157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1662552, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000506 DASTJJ010000000
VERSION     DASTJJ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662552"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1157
                     /locus_tag="VFR02_06220"
     CDS             <1..>1157
                     /locus_tag="VFR02_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9870074.1"
                     /translation="LGPFGGLAPNQALLALQGFTAVTSVSSLALAAAILERNLAEGNL
                     HKARRAEQALEDSEKYYRALIENSLDVVTLLDAQGTVLYESPSILPVLGYGTRELVGT
                     DVFRLIHPEDAPGVREKFKALLTEPAETQSARFRFRHKDGSWRDLESFGRNALGEAAV
                     GGIIVNSRDITERQAARLFLWESEERFRQLSESTREGVVISREGRILATNSVFARMFG
                     YDPEEVRGKSLADLLGGRDPALPEAGAGRAIEFNGVRKDGSTFPAEAAGKPVSFEGRE
                     ARVDCVTDLTRQKNDEKELREQAGLMRSIVDNMGEGLLVCNEKGKTLFVNPAAQAILG
                     GQVGDPLPGNEGPKNLVSLGEEGPPLSPGEFPITRALAGESSDNVELFIRN"
BASE COUNT          193 a          369 c          422 g          173 t
ORIGIN      
        1 cctggggccc ttcggcggcc tggcgccgaa ccaggccctt ttggcgctgc agggtttcac
       61 cgccgtcacc tcggtctcct ccttggcctt ggcggcggcc atcttggagc ggaacctggc
      121 ggaggggaac ctgcataagg cccggcgggc cgagcaggcg ctggaggaca gcgaaaaata
      181 ttaccgggcc ctgatcgaga actcgctgga tgtggtgacc ctgctggacg cgcaggggac
      241 ggtcctctat gagagcccca gcatcctgcc cgtgctgggt tatgggacgc gggaattggt
      301 ggggacggac gtcttccgcc tgatccaccc cgaggacgcg cccggggtgc gggaaaaatt
      361 caaggccctg ctgacggaac ccgccgagac ccagtcggcc cggttccggt tccggcacaa
      421 ggacgggtct tggcgggacc tggaatcctt cggccgcaac gccttggggg aggcggccgt
      481 cgggggcatc atcgtcaatt cgcgggacat caccgagcgc caggccgccc gccttttcct
      541 gtgggaaagc gaggaacgct tccggcaatt gtccgaatcc acgcgcgagg gggtggtcat
      601 cagccgggaa ggccgcatcc tggcgaccaa ctcggtcttc gcccggatgt tcggctacga
      661 cccggaagag gtccgcggca agtccctggc ggacctgctg ggggggcggg acccggccct
      721 ccccgaggcc ggggccgggc gggccattga gttcaacggc gtgcgcaagg acgggtcgac
      781 cttcccggcg gaagccgccg gcaaacccgt ttccttcgag gggcgcgagg cccgggtgga
      841 ttgcgtgacg gacctgacgc gccaaaagaa cgacgaaaag gaactgcgcg agcaggcggg
      901 cctgatgcgg tcgatcgtgg acaacatggg ggaaggcctg ttggtctgca atgagaaggg
      961 caagaccctc ttcgtgaacc ccgccgccca ggccatcctg ggcgggcagg tcggggaccc
     1021 ccttccgggg aacgaggggc ccaagaacct ggtcagcctg ggcgaggagg ggccgccgct
     1081 gtcgcccggg gaattcccca tcacgcgggc cctggcgggg gagtcctccg acaacgtgga
     1141 gcttttcatc cgcaacc
//
LOCUS       DASTJJ010000507         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_317426, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000507 DASTJJ010000000
VERSION     DASTJJ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317426"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..>1057
                     /locus_tag="VFR02_06225"
     CDS             130..>1057
                     /locus_tag="VFR02_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9870075.1"
                     /translation="MPLRAMNRDQEWLFPPSMDDLVGEDHPARFVAAFVDALGGDTLR
                     EMGIVAEGDALGAPSYPPRLLLSIWLYGFLRGVRSSRKLEVACREHLPFLWLSGFTHP
                     DHNTLWRFYQAHRQSMRTLLKRTVRAAVRVGLVDLAVQAVDGTKVMGNVAKERTLDQE
                     GLSKLLERTEAAIADLEAQNTTGGDSPPPSLPPTLCQKRELRTQVQEALKQIQAEEGR
                     ERVNLTDPEAVLLKGRAGYLTGYNAQAMVSPLQEEQAGGSGLLISAAEITTDPDDHRQ
                     LLPMIHEARANLGTDPGLTLADGGSHSGENLQA"
BASE COUNT          202 a          313 c          365 g          177 t
ORIGIN      
        1 atttcattct gactccctgc ccaaccattc gtaggggcgg gcctccgtga gcgtgtctca
       61 aaagggaggg acttccggtc ggaaggggga gcggctatac tggaggtgaa gaagcgaggg
      121 aggagagaga tgcctctacg cgcgatgaac cgcgaccagg agtggctgtt tcccccatca
      181 atggatgatt tggtgggtga ggatcatccg gcccgcttcg tggcggcgtt tgtggatgcc
      241 ctgggagggg acaccctgcg ggagatgggc atcgtggcgg agggcgacgc gttgggagcg
      301 ccgtcgtacc caccgcggct gctcctcagc atctggctct acggcttcct gcgtggggtg
      361 cgctcctcgc gcaagctcga agttgcctgc cgcgaacacc tccccttcct gtggttgagc
      421 gggttcactc atcccgacca caacacgtta tggcgcttct accaggccca tcgccaatcg
      481 atgcggacgc tactcaaacg gacggtccgc gcggcggtgc gggtggggtt ggtcgatttg
      541 gcggtgcagg cagtggatgg cacgaaagtg atgggcaatg tcgccaagga gcgaaccctg
      601 gaccaagaag gcttaagcaa actcctggaa cggaccgagg cggcgatcgc cgacttggag
      661 gcccagaaca cgaccggggg cgattcccct ccaccctcct tgcctcccac cttgtgccag
      721 aagcgggagt tgcgcaccca ggttcaggaa gcgttgaagc agatccaggc ggaggaggga
      781 cgcgaacggg tgaacctgac cgatccggag gcggtgttgc tgaaaggacg ggcggggtac
      841 ctaacgggct acaatgccca ggcgatggtc tcaccgctcc aagaagagca ggcaggaggg
      901 agcgggttgc tgatcagcgc cgctgagatc accaccgatc ccgacgacca ccgccagctc
      961 ctcccgatga tccacgaggc ccgagcgaac ctggggacgg atcctggcct caccctggcc
     1021 gacgggggct ctcattcggg ggagaacctc caggcgt
//
LOCUS       DASTJJ010000508         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_72170, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000508 DASTJJ010000000
VERSION     DASTJJ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72170"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFR02_06230"
     CDS             <1..252
                     /locus_tag="VFR02_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870076.1"
                     /translation="STNVQVTQVGDYIFRIFKRLHTREEFPGAGLGLAVCRKSVDIHG
                     GRIWCESEPGKGSSFYFSIPAKVRDPAVPQAVEAGPGPE"
     gene            352..1491
                     /locus_tag="VFR02_06235"
     CDS             352..1491
                     /locus_tag="VFR02_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9870077.1"
                     /translation="MQRSFLLMSALCLLAAGSLVSCGPKVDPNEIVIGEYGSLTGSDA
                     TFGQSNHEGVMMAVDEINAAGGVQGKTLRDISLDDEGKPEEAALAVTKLITEDHAQVV
                     IGEVASSRSLAAAPICQQHQVPMISPSSTNVQVTQVGDYIFRMCFIDPFQGQVMADFA
                     RGKLKARTAAIFRDQKSDYSEGLADAFVKRFKAKGGVIVTDQSYVAGDIDFKSQLTTI
                     RGKKPDVIFVPGYYTEVGLIAQQARELGIRVPLLGGDGWDSSKLFEIGGAALNGCYFS
                     NHYSSQSTDPRVMDFVKKYQDRYHHTPDALATLAYDATGYLAAALKTAKGFTGPQIRD
                     ALASLKQYDGVTGVIRLDADRNPIKPAVVLKIEDGKASYVTTVKP"
BASE COUNT          270 a          535 c          467 g          242 t
ORIGIN      
        1 tccaccaacg tgcaggtcac ccaggtgggc gactacatct tccgcatctt caagcgcctg
       61 catacccgcg aggaattccc gggggcgggc ctggggctgg ccgtctgccg caagagcgtg
      121 gacatccacg gcggccgcat ctggtgcgaa tccgagcccg gcaagggctc ctctttttat
      181 ttttccatcc ccgccaaggt ccgggacccg gcggtccccc aggcggtgga ggcgggcccc
      241 ggcccggaat gaccgccccg ccggtgcggc gtccttgaca gcccccccgg gggcttttta
      301 tcctacctgc accccacgcc gaccgtggga ccatccccca cgaggtgacc gatgcaacgt
      361 tccttcttat tgatgagcgc cctgtgcctg ttggcggcgg gcagcctggt ttcctgcggc
      421 cccaaggtcg accccaacga gatcgtcatc ggcgaatacg gctccctgac cgggtccgac
      481 gccaccttcg gccagtccaa ccatgagggc gtgatgatgg ccgtggacga gatcaacgcg
      541 gccggcggcg tgcagggcaa gaccctgcgg gacatcagcc tggacgacga gggcaagccc
      601 gaggaggcgg ccctggccgt caccaagctc atcaccgagg accacgccca ggtcgtcatc
      661 ggcgaggtgg ccagttcccg gtcgttggcg gcggcgccca tctgccagca gcaccaggtc
      721 cccatgatct cgccctcctc caccaacgtg caggtcaccc aggtgggcga ctacatcttc
      781 cgcatgtgct tcatcgaccc cttccagggc caggtcatgg ccgatttcgc ccgggggaag
      841 ttgaaggcca ggacggcggc catcttccgc gaccagaaga gcgactacag cgagggcctg
      901 gcggacgcct tcgtcaagcg cttcaaggcc aagggcgggg tgatcgtgac cgaccagagc
      961 tacgtggcgg gcgacatcga cttcaaatcg cagctcacca ccatccgggg caagaagccc
     1021 gacgtcatct tcgtgcccgg ctattacacc gaggtggggc tcatcgccca gcaggcccgc
     1081 gaactgggga tccgggtgcc cctcttgggg ggcgacggtt gggactcgtc caagcttttt
     1141 gagatcggcg gcgcggcgct gaacggctgc tacttctcca accattattc ctcgcagtcc
     1201 accgaccccc gggtgatgga cttcgtgaaa aaatatcagg accgttacca ccacacgccc
     1261 gatgccctgg ccacgctggc ctacgacgcc accgggtacc tggccgccgc cttgaagacc
     1321 gccaaaggct tcaccggccc ccagatccgc gacgccttgg cgtcgctgaa acaatatgac
     1381 ggcgtcaccg gcgtcatccg gctggatgcc gaccgcaacc ccatcaagcc cgccgtggtg
     1441 ctgaagatcg aggacggcaa ggcctcctac gtgaccacgg tgaagcccta aagggcctcc
     1501 cgggccccga aaag
//
LOCUS       DASTJJ010000509         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_560394, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000509 DASTJJ010000000
VERSION     DASTJJ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560394"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFR02_06240"
     CDS             <1..222
                     /locus_tag="VFR02_06240"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181015.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9870078.1"
                     /translation="EGIFAETAGGVTVACLKKLAASGALDPDAVTVAYITGTGLKTQE
                     AVADNLRSVPVIQPNLDAFEKTLLEPAGR"
     gene            305..586
                     /locus_tag="VFR02_06245"
     CDS             305..586
                     /locus_tag="VFR02_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9870079.1"
                     /translation="MAVKVLIPTPLRNLTQNEAEVSADGATIQEIIDDLGRKYAGLKD
                     RLCDEKGKIRRFVNLYLNEEDIRFLQGEATAVKAGDQVSIVPAIAGGRN"
     gene            630..875
                     /locus_tag="VFR02_06250"
     CDS             630..875
                     /locus_tag="VFR02_06250"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HET9870080.1"
                     /translation="MGLMKKLIELDYPTGLVTEPVLSLLIRKHDILLNIRRASITKGF
                     GYVQAEISGEESEVKKALADLTAQGIDVNPIEKDVVE"
     gene            881..1546
                     /locus_tag="VFR02_06255"
     CDS             881..1546
                     /locus_tag="VFR02_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870081.1"
                     /translation="MEPSDWQERFSRQTILASVGEAGQRKWARSSLGVLGGGTALESA
                     LTALISSGLTRLRLGVPGTFDLPAWQHRFPEADLARLDPAADGKHLDGFLVLTEDEAE
                     RRRWSRSLRAQGRPAFFGWTAGSGTALFFAPHASSDCPCFECFEVLNPKTFQGGTAET
                     RRLLGALAAAEVLQWLVKGETPLANQVWITSLEGGLSVRHPVEPSAKCPAGLLARGAK
                     VTP"
BASE COUNT          269 a          539 c          524 g          249 t
ORIGIN      
        1 gagggcatct tcgccgagac cgccggcggg gtgaccgtgg cctgcctgaa gaagctggcc
       61 gcctcgggcg cgttggaccc ggacgccgtc acggtggcct acatcacggg cacgggcctc
      121 aagacccagg aggcggtggc cgacaacctg cgttcggtgc cggtgatcca gcccaacctg
      181 gacgccttcg aaaagaccct gttggagccg gcgggccgct gaaatttggg gggattttcc
      241 actggaaaat cccttcgggc ttcgtccata atccagtgac tcaagaccgc ccaggaggca
      301 gttcatggcc gtgaaagtgc tcattcccac ccccttgcgc aacctgaccc agaacgaggc
      361 ggaagtctcg gccgacgggg ccaccatcca ggagatcatc gacgacctgg gccgcaagta
      421 cgccgggctg aaggaccgcc tttgcgacga gaagggcaag atccgccgct tcgtgaacct
      481 gtacctcaac gaggaggaca tccgtttcct gcagggcgag gccacggccg tgaaggccgg
      541 ggaccaggtg tccatcgtac ccgccatcgc gggcggccgg aactgaccct ccctcagggc
      601 gcggcgatcc atctttggag gggtgcccca tgggcctcat gaaaaaactc atcgaattgg
      661 attatcccac cggcctggtc accgagccgg tcctgtccct gttgatccgc aagcacgaca
      721 tcctgctcaa catccgccgg gccagcatca ccaagggctt cggttacgtc caggccgaga
      781 tctcgggcga ggagtccgag gtcaagaagg ccctggccga cctgaccgcc cagggcatcg
      841 acgtcaaccc catcgaaaaa gacgtcgtcg agtaatccgg ttggaacctt ccgactggca
      901 ggagcggttc agccgccaaa cgatcctggc gtccgtgggg gaagcggggc agcgcaaatg
      961 ggcccggtcc tccctgggcg tcctgggcgg ggggacggcg ctggaatccg ccttgacggc
     1021 cctgatcagt tcgggcctga cccgcttgcg cctgggggtc cccgggacct tcgatctccc
     1081 cgcctggcag caccgtttcc ccgaagccga cctggcccgc ctggaccccg ccgccgacgg
     1141 caagcacctg gacggcttcc tggtcctgac cgaggacgag gccgaacggc ggcggtggag
     1201 ccgttcgctg cgcgcccagg gccgcccggc ctttttcggc tggaccgcgg gcagcgggac
     1261 cgccctcttt ttcgcccccc acgcctcctc ggattgcccc tgtttcgagt gtttcgaggt
     1321 cctgaacccg aagacgttcc aaggggggac ggcggagacc cgccgcctgt tgggcgcgtt
     1381 ggcggcggcg gaggtcctgc agtggctcgt gaagggggaa acgcccttgg ccaaccaggt
     1441 ctggatcacg tcgttggagg gtgggctgtc ggtccgccat ccggtggagc cgtccgccaa
     1501 atgcccggcg ggcctgctgg cccgcggcgc gaaggtgacc ccatgagagg accccaacca
     1561 tgagcgaccc ccaaccgctc c
//
LOCUS       DASTJJ010000510         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_804729, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000510 DASTJJ010000000
VERSION     DASTJJ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804729"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VFR02_06260"
     CDS             <1..1300
                     /locus_tag="VFR02_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870082.1"
                     /translation="YETVRLRKDGRPLAVAVNLCPIRNPQGGVTGISVIYRDLTRERE
                     AQAALKDQEEQLRLAQKMDAIGRLAGGVAHDFNNLLSVIGGNAEFLLSSLPAEDPQRE
                     ELTEIQKAVKRGADLTRQLLLFGQKQVAQPASLQLNDVMGEMHKMFRRLIDAHVEILM
                     EPEPGLWRIQADPLQLQQVLLNLALNARDAMPRGGRLVLRTRNVGSSDPELKDNPDLR
                     AGGYVKLQVTDTGVGMTPEVQRRVFEPFFTTKADKGTGLGLSTVYGIVRQADGHIRLE
                     SHPGLGTTFSIFFPAQHEPPAPERSEQIPLLPLGDETILVAEDEEPVRKIVMRILEKQ
                     GYRLLEADQGEAALRKAEGTDGPIHLLLTDTVMPKMNGKELAQKLKKLRPKVKVLFMS
                     GYPQEVLARQGTLEPGIHLLQKPFTAEELARKVRQILDGR"
     gene            complement(1297..1779)
                     /locus_tag="VFR02_06265"
     CDS             complement(1297..1779)
                     /locus_tag="VFR02_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870083.1"
                     /translation="MKTMREEGQSCLSRFPAPEDLVLLFERFMAYWCGERVKLAGLGL
                     GAPGPGSPEFMVRWRGAVPGTLSLRFDPGFYPWLVRRRDFKHMNLYTEDALLQEITAL
                     YGIYLIQYFSMADLADAGPLLPRRAPPGQLPARGPDSQIGIRVEECPLEIRFWAGDGP
                     "
     gene            complement(1924..>2377)
                     /locus_tag="VFR02_06270"
     CDS             complement(1924..>2377)
                     /locus_tag="VFR02_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9870084.1"
                     /translation="ADLYYRLNVIPFTLPPLRSRPDDIAQLANFFLQESCQAFDKKLS
                     LLSDEVLQVLKNYPWPGNVRELKHVIERLVVTAKGKSLAVKDLPKEVREAKAGPMTTR
                     GSLPKAEAEKQNIQRALLETNGNKSKAADRLGITRKTLFNKMKRYNLK"
BASE COUNT          438 a          772 c          787 g          380 t
ORIGIN      
        1 ctacgagacg gtccgcctgc gcaaggacgg gcggcccctg gccgtggcgg tgaacctctg
       61 ccccatccgg aacccgcagg gcggggtgac gggcatctcg gtcatttacc gcgacctgac
      121 gcgcgagcgg gaggcccagg ccgccctgaa ggaccaggag gaacagctgc ggctggccca
      181 aaagatggac gccatcggcc ggttggccgg gggcgtggcc cacgacttca acaacctctt
      241 gagcgtcatc ggcggcaacg ccgaattcct cttgtccagc cttcccgccg aggaccccca
      301 gcgggaggaa ctgaccgaga tccagaaggc ggtgaagcgg ggcgcggacc tgacccgcca
      361 actgctgctc ttcggccaga agcaggtggc ccaacccgcc tcgttgcagt tgaacgacgt
      421 catgggcgag atgcacaaga tgttccggcg cctcatcgac gcccacgtgg agatcctgat
      481 ggagcccgaa ccgggcctgt ggcgcatcca ggccgacccc ctccaactgc agcaggtcct
      541 cctcaacctg gccttgaacg cccgggacgc catgccccgg ggcgggcggc tggtcttgcg
      601 cacccgcaac gtcgggtcct cggaccccga gctgaaggac aacccggacc tgcgggcggg
      661 cggctacgtg aaactgcagg tgaccgacac cggggtgggc atgacccccg aggtgcagcg
      721 gcgggtcttc gagccctttt tcaccaccaa ggccgacaag ggcacgggcc tgggcctttc
      781 cacggtctat ggcatcgtgc ggcaggccga cggccacatc cgcctggaaa gccatccggg
      841 cttaggcacc accttctcga tcttttttcc ggcccagcat gagcccccgg ccccggaaag
      901 gtccgagcag atccccctgc ttcccctggg ggacgagacc atcctggtgg cggaggacga
      961 ggagccggtg cgcaagatcg tcatgcgcat cctggaaaag caggggtacc ggctgttgga
     1021 ggccgaccag ggggaggcgg ccttacggaa ggccgagggg acggacgggc ccatccacct
     1081 actcttgacc gatacggtca tgcccaagat gaacggcaag gaattggccc agaagctcaa
     1141 gaagctcagg cccaaggtca aggtgctctt catgtccggc tatccccagg aagtattggc
     1201 ccgccagggc accctggaac ccggcatcca cctgctgcaa aaacccttca ccgccgagga
     1261 actggcccgg aaggtgcgcc agatcctgga cggccgttaa ggcccgtcgc ccgcccagaa
     1321 ccggatctcc agggggcatt cctccacccg gatgccgatc tgggaatcgg ggccgcgggc
     1381 cggcagctgc ccgggcggcg cgcggcgggg cagcaggggc cccgcgtcgg caaggtcggc
     1441 catggaaaag tactggatca ggtagatgcc gtagagcgcc gtgatctcct gcagcaaggc
     1501 gtcctcggtg taaaggttca tgtgcttgaa gtcccgccgg cgcaccagcc aggggtagaa
     1561 gccggggtcg aaacgcaggc tcaaggtccc cgggaccgcc ccccgccacc gcaccatgaa
     1621 ttccggcgat ccgggcccgg gggcccccag gcccaagccc gccagcttca cccgttcccc
     1681 gcaccaatag gccatgaagc gctcgaaaag gagcaccaag tcctccgggg cggggaaccg
     1741 gctcaaacag ctttggcctt cttcccgcat ggtcttcatg gcgccctcct tgcctgttcc
     1801 ctcaaaaggg aaggaagcaa gaccggtgcc agggacggga ggggggaaat gcgggaaaaa
     1861 tgaaaagggg cgccgcgggc gggcggatac tttattccca gcgggaatct tttgcccggc
     1921 gccttatttc aggttgtagc gtttcatctt gttgaagagg gtcttgcggg tgatgcccag
     1981 gcggtcggcg gccttggatt tgttgccgtt ggtctccagc aaggcccgtt ggatgttctg
     2041 cttttcggct tccgccttgg gcagggaacc ccgggtggtc atgggcccgg ccttggcctc
     2101 gcggacctcc ttgggaaggt ccttcacggc caaggatttg cccttggcgg tcaccaccag
     2161 ccgctcgatg acgtgcttca attcccgcac gttccccggc caggggtagt tcttcagcac
     2221 ctgcaggacc tcatcggaca gcaggctcaa tttcttgtcg aaggcctggc aggattcctg
     2281 caggaagaaa ttagccaatt gggcgatgtc gtcgggccgg gacctgaggg gcggcagggt
     2341 gaaagggata acgttcaacc ggtagtaaag gtccgcc
//
LOCUS       DASTJJ010000511         1118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_439126, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000511 DASTJJ010000000
VERSION     DASTJJ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439126"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..132)
                     /locus_tag="VFR02_06275"
     tRNA            complement(58..132)
                     /locus_tag="VFR02_06275"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Gly,seq:gcc)
     gene            complement(243..>1118)
                     /locus_tag="VFR02_06280"
     CDS             complement(243..>1118)
                     /locus_tag="VFR02_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9870085.1"
                     /translation="SGMDPGGLPAPERLFRGFMSYEQFYGLREQAFSNAPDSRFYFES
                     SQHSEAMVRMLHAVDTMKGLTVVVGDIGAGKTLLARKALEKLEQDHQYEATLLVIVHT
                     EITAEWMLKKIAVQLGVENPGETKGQIISQLYERLMKIYEEGRKAVVLIDEANMLQKK
                     EIFEEFRGLLNLEVPGRKLISIVLLGLPELEQYLALDPPLFQRIAVKFFVKSLTREST
                     ENYIQHRLSVAGCAKPVFSQEAFQKIFDYTRGIPRLINTACDNAMLEGYLLKKETIDG
                     DIIDQVAKDLGLKKE"
BASE COUNT          214 a          325 c          341 g          238 t
ORIGIN      
        1 cggaagccga gatgggaaaa ttgggggaaa cgggaagccc taccgatggt gaaaaaatgg
       61 agcgggaaac gggattcgaa cccgcgactt caaccttggc aaggttgcac tctaccactg
      121 agttattccc gcgttttcca cgaacagaac ttccaaagcg gagcggatta taccggcggg
      181 cggggtccac gtcaattggg gaaatcaggg ctggacgcgg cgccgaaagc ctccccaagg
      241 gcctattcct tctttaatcc gaggtccttg gcgacttggt cgatgatgtc cccgtcgatg
      301 gtttcctttt tgagcaggta gccttccagc atggcgttgt cgcaggccgt gttgatgagc
      361 cgggggatgc cccgggtgta gtcgaagatc ttctggaagg cctcctggct gaagaccggc
      421 ttggcgcagc ccgccaccga caggcggtgc tggatgtagt tctcggtgct ttcccgggtg
      481 agcgacttca cgaagaactt gacggcgatg cgctggaaca gcgggggatc cagggccaag
      541 tactgctcca attcgggaag ccccaacagc acgatggaga tcaatttgcg gcccggcacc
      601 tccaggttca acaggccccg gaactcctcg aagatctcct tcttttggag catgttggcc
      661 tcgtcgatca gcaccacggc cttgcgccct tcctcgtaga tcttcatcaa ccgctcgtag
      721 agctgggaga tgatctggcc cttggtctca ccggggttct ccacccccaa ttggaccgcg
      781 atcttcttga gcatccattc ggcggtgatc tcggtgtgca cgatgaccaa cagggtggcc
      841 tcgtattggt ggtcctgttc gagcttttcg agggccttgc gggccagcaa tgtcttcccc
      901 gcgccgatgt cccccaccac cacggtcaac cccttcatgg tgtccacggc gtggagcatg
      961 cgcaccatgg cttccgagtg ctggctgctt tcgaagtaga agcggctgtc gggcgcgttg
     1021 gaaaaagcct gttcccgcag accgtagaat tgttcgtagc tcataaaccc tcggaaaagg
     1081 cgttcggggg cggggagccc gccgggatcc atcccgct
//
LOCUS       DASTJJ010000512         1902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_683449, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000512 DASTJJ010000000
VERSION     DASTJJ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683449"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFR02_06285"
     CDS             complement(<1..300)
                     /locus_tag="VFR02_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HET9870086.1"
                     /translation="MRPRYAAPLLLLSFLAASRVLAQDPGTTGADILTTPVGVRPAAM
                     GGTYAAFGDDVYVIGYNPAGLAGVTKYSLGMDHRDGLAGVQTEALSLAIPTRDYGN"
     gene            complement(350..1819)
                     /locus_tag="VFR02_06290"
     CDS             complement(350..1819)
                     /locus_tag="VFR02_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870087.1"
                     /translation="MSLVWIFLCPAARAQFASVPNDGFLTPGGSDDRGAVAEGARSFS
                     LEEGGWNSLQGLLASQVGWAGTGLRAIAPGLLFEGVAYPLDDGRILGWAPAWGRVELL
                     DFPAQAWWGPSAAAGAVQWRAPAFTDDPRAGFTAGGGTGGFGEVEGDYSTTGLGLSAG
                     YRRAPGSPFTAGPLDAGGLQASARIPGTGDWGLGGSFLAVGDGAGDDWIVGLGDLRWT
                     DGNFQRLRLRPYAQSARSDGGEVREAGADLDYDLDLAGIAEANLAGGVSRREGPGART
                     AGFLQDSELLDALGDLTLDGAFRLDWTGDGSALFSTVLGAQMAVGDMLLRADEATAQD
                     PADPGRPVEQAELGLRPRGDDKSFDLAYRHLSTSQAVLNGVALGAGRDLDFLEGPVLG
                     QASVGVQLQYLVDTAGTGRADFGAWADGFWFGALRTRVLARRTGDGPLFGSARVSCRL
                     DPRSEVFLEGQNLGGLPQAWPEPDWRPGLDILGGVHLDL"
BASE COUNT          326 a          725 c          638 g          213 t
ORIGIN      
        1 attgccgtag tcccgggtgg ggatggccag gctcagggcc tcggtctgca cccccgccaa
       61 gccgtcccgg tggtccatcc ccaggctgta tttggtcacc ccggccaggc cggcggggtt
      121 gtagccgatg acatagacgt cgtcgccgaa ggccgcgtag gtgccgccca tggcggccgg
      181 acggacgccc acgggcgtcg tcaggatgtc cgcccccgtg gtgcccgggt cctgggcgag
      241 aacgcgggac gcggccagga aggacaacag caggaggggc gcggcatagc ggggacgcat
      301 gggtcaaaac tatcacgcgg cccccggggg gtcaagaagg gcccggggcc taaaggtcca
      361 ggtggacgcc gcccaggata tccagccccg gccgccaatc cggctcgggc caggcttggg
      421 ggaggccgcc caggttctgg ccttccagga agacctcgct gcgcgggtcc aggcggcagg
      481 aaacccgggc cgacccgaaa agcggcccgt ccccggtccg gcgcgccagc acccgggtcc
      541 gcagggcgcc gaaccaaaag ccgtcggccc aggcgccgaa gtcggcccgc ccggtccccg
      601 ccgtatcgac cagatactgc agctggaccc cgaccgaggc ttgccccagc acgggcccct
      661 ccaggaagtc caggtcccgt ccggccccca gcgccacccc gttgagcacc gcctgggaag
      721 tggacaggtg ccggtaggcc aggtcgaagc ttttgtcatc cccccgcggc cgcaggccca
      781 gttcagcctg ttccaccggg cggcccgggt ccgccggatc ctgggccgtg gcctcgtccg
      841 cgcgcagcag catgtccccc accgccatct gggcccccaa gaccgttgaa aaaagggccg
      901 acccgtcccc cgtccaatcc agccggaagg cgccgtccag ggtcaggtcc cccagggcgt
      961 ccaacagctc actgtcctgc aggaaacccg ccgtgcgggc gcccggtccc tcgcggcggc
     1021 tgacgccgcc cgccaagttc gcctcggcga tgcccgccag gtccaggtca tagtccaggt
     1081 ccgctcccgc ttcccgcacc tccccgccgt ccgaccgcgc gctctgcgcg tagggcctga
     1141 ggcgcaggcg ttggaaattg ccgtcggtcc accgcaggtc ccccaggccg acgatccagt
     1201 cgtcccccgc gccgtcgccc accgccagga acgacccgcc caggccccag tcccccgttc
     1261 cggggatccg cgcggaagct tgaaggcccc cggcgtccag ggggcccgcg gtgaagggcg
     1321 atccgggcgc gcggcggtaa ccggccgaaa gcccgaggcc ggtcgtcgag tagtccccct
     1381 ccacctcccc gaagccgccc gtgcctccac ccgccgtgaa gccggcccgc ggatcgtcgg
     1441 tgaaggcggg ggcccgccac tggaccgcgc ccgcggccgc cgagggaccc caccaggcct
     1501 gggcgggaaa gtccaaaagt tccacccggc cccaggccgg ggcccaaccc aggatgcgcc
     1561 catcgtccag cggataagcc acgccctcaa agagcagtcc cggcgcgatc gcgcgcaatc
     1621 cggtcccggc ccatcccacc tgggacgcca aaagaccctg cagcgagttc caaccgccct
     1681 cctcgaggct gaaggaccgc gccccctcgg ccaccgcgcc ccggtcatcg gacccgccgg
     1741 gggtcaggaa accgtcattg ggaacggagg cgaattgggc ccgggccgcc gggcaaagga
     1801 agatccaaac cagggacaaa aaaaaaggaa agggaccgga acggacggga aaggggcttc
     1861 ggggaaaaag gaagatccgt aggacaacac cttagaaaaa tg
//
LOCUS       DASTJJ010000513         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1540088, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000513 DASTJJ010000000
VERSION     DASTJJ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540088"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFR02_06295"
     CDS             <1..423
                     /locus_tag="VFR02_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870088.1"
                     /translation="TGPGIGPRIGKKYLKGIGMRKNSGLAAVLSFFICGLGQIYNGEI
                     GKSLIMFFIYGLGAWATFQLSLLWVLPCVGFWIYGIIDAYSTAEEITDSVIRFEARQR
                     QDAEKSSMLSTASSQVSQGHSRRRVNPKIANQNFKPID"
     gene            597..1451
                     /locus_tag="VFR02_06300"
     CDS             597..1451
                     /locus_tag="VFR02_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870089.1"
                     /translation="MRKALVGLGLLVLLGQGTAAQAAGNNAMAGPSASMTTTDMALGG
                     GSGLDLKLYSGVDYSFLGDFVNGTKAFAQYLTDSGGEATAWTGNWGWVSGLDLGLSLD
                     PSDRISVGFESVLTRSQNISGTDGSAGVGGSDTPQMFNATLNYGLSIPEGKGTRTFFT
                     VGAGYYHAFLDYNLGVAAQDTYGTFTGDTVGGTLGVGQEWSVGGGFDLSFSAKGRYAV
                     FDKLTATSFNDANNPTPYALAMISIPGEPDILLPQATSNIDANSTMRYAKLDYCGVDA
                     TVSMSYHF"
     gene            1662..1738
                     /locus_tag="VFR02_06305"
     tRNA            1662..1738
                     /locus_tag="VFR02_06305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1696..1698,aa:His,seq:gtg)
     gene            1832..1908
                     /locus_tag="VFR02_06310"
     tRNA            1832..1908
                     /locus_tag="VFR02_06310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1866..1868,aa:Arg,seq:ccg)
     gene            1926..2001
                     /locus_tag="VFR02_06315"
     tRNA            1926..2001
                     /locus_tag="VFR02_06315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1959..1961,aa:Lys,seq:ctt)
     gene            2155..>2940
                     /locus_tag="VFR02_06320"
     CDS             2155..>2940
                     /locus_tag="VFR02_06320"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET9870090.1"
                     /translation="MRYGYFDVPNKEYVIERPDTPVPWMNYLHNDEYCALISNNGGGY
                     SFHLSARDKRILRYRLNNVPMDRPGRYIYIRDEKTKDFWSATWQPVLKDLSEYKTETR
                     HGFGYTRVTSSYKGIESDTLYVVPVDDNLELWHIALKNQTSTTKTLTLTSYAELCLFF
                     AQNDMINFQYTYNIAKAYEKNNIIHHTTMIESAGHYAFFSADKKFASYDCDREAFMGL
                     YHSEANPQAVLAGKCSGSTADGGNPVAATTLSVTLKPGETKTVT"
BASE COUNT          613 a          892 c          822 g          613 t
ORIGIN      
        1 acgggtcccg gaattggtcc aagaattgga aaaaaatatc taaagggaat agggatgaga
       61 aaaaattcag ggttggctgc agttctgtct ttttttatat gcgggcttgg ccagatatac
      121 aatggagaaa tcggcaagag cctgattatg tttttcattt atgggctcgg ggcttgggcg
      181 acatttcaac tttccttgct ttgggttttg ccctgtgtcg gcttttggat atacggaatt
      241 attgatgcct attcgacggc ggaagaaatc actgatagtg tcatccgatt tgaagcgcgc
      301 cagagacaag atgcggaaaa gagctcaatg ttatcaacag catcgagcca ggtttcgcaa
      361 ggccattcaa gacgcagggt caaccccaaa attgcgaacc agaattttaa gccgattgat
      421 tgaggacttc caggcgcacc attttttcca ccatcgaacg ttccagtgtt tcgttttcct
      481 tcgcgctatt cccggccccg gcggccatac ccccttttgg tgtagtgtcg cttccggaaa
      541 gatgatgcta cattccgccc accttggact ttggtccatc atgaggaggg gggcatatgc
      601 ggaaggcact ggtggggttg ggattgttgg tgttgctggg acaaggaacg gcggcccagg
      661 cggccgggaa caatgccatg gcggggccgt cggcctcgat gaccacgacc gacatggcgc
      721 tgggaggcgg atcgggcctg gacctgaaac tctattcggg cgtggactat tctttcctgg
      781 gcgacttcgt caacggcacc aaggcgttcg cccaatacct cacggacagc ggcggagaag
      841 ccacggcttg gaccgggaac tggggttggg tgtcgggtct ggaccttggc ctctccctgg
      901 acccttccga ccgtatctcg gtggggtttg agagcgtctt gacccgcagc cagaacatca
      961 gcgggaccga tgggagcgcc ggggtcggcg gttcggacac cccccagatg ttcaacgcga
     1021 ccctgaatta cggcctttcc atcccggaag gaaaagggac ccggaccttc ttcactgtcg
     1081 gcgcgggcta ctaccatgcc ttcctggact ataatctggg ggtggccgcg caggacacct
     1141 acgggacctt tacgggcgac acggtgggcg gcaccctggg ggtgggccag gaatggtccg
     1201 tcggcggcgg gttcgacttg tccttttccg ccaagggccg ctatgcggtc ttcgacaaac
     1261 tgaccgccac gtccttcaat gacgccaaca acccgacgcc ttacgccctg gccatgatca
     1321 gcatccccgg ggaaccggac atcctgctcc cccaagccac gtccaacatc gatgccaact
     1381 ccacgatgcg gtacgccaaa ctggactact gcggcgtgga cgccacggtc tcgatgtcct
     1441 accacttctg accctaaggc cgcttccaac cggtcgaaaa gaaggggacc tccctacagg
     1501 tccccttctt ttttgccgtc cgctttggcc gaaaaggccc atccgaggcc cttttccccg
     1561 aaatttgacc tttacatttg tgacccccac ttttaaactc tctttcccga atcggccctt
     1621 cgggccttct cgggcgtttc ccaagccaaa ccccggacat ggtgggagta gctcaattgg
     1681 ttagagcacc agattgtgga tctgggggtt gcgggttcaa gtcccgtctc tcaccccatc
     1741 tatgttggac ggggcgcagc caagggaccg tgaacgtcgg gaagtcggaa gggtagggaa
     1801 tggccccgga cgtccggagc cggtaatcga tgcgcctgta gctcagttgg atagagcgac
     1861 agattccgga tctgtaggtc agaggttcga gccctctcag gcgtaccatt ttttgttttt
     1921 ttgacgggtg attagctcag ttggtagagc agctgactct taatcagcgg gtcgcaggtt
     1981 cgagccctgc atcacccacc attttgcacc catccctcgc gggaagggtc ggggggaacg
     2041 ggagtttccc cggttgtttt cggcatccac gcggtccgcg tgaattaaat cggttttatg
     2101 gtgtcagggt ccagtgcgtc ggtgagggtt accactacta aaaagaggaa gaccatgcgc
     2161 tacggttact tcgacgtccc gaataaggaa tacgtgattg agcggcccga cacgcccgtg
     2221 ccctggatga actacctcca caacgacgag tactgcgccc tcatttccaa caacggcggc
     2281 ggctacagct tccacctctc cgcccgcgac aagcgcatcc tgcgctaccg cctgaacaac
     2341 gtgcccatgg accgcccggg ccgctacatc tacatccgcg acgagaagac caaggatttc
     2401 tggtccgcca cctggcagcc cgtcctgaag gacctatccg agtacaagac cgagacccgc
     2461 cacgggttcg gctacacccg ggtcacgtcc agctacaagg gcatcgagtc cgacaccctc
     2521 tacgtggtgc cggtggacga caacctggaa ctgtggcaca tcgccctcaa gaaccagacc
     2581 tccacgacca agaccctcac cctgacctcc tacgccgaac tgtgcctgtt cttcgcccag
     2641 aacgacatga tcaacttcca gtacacctac aacatcgcca aggcctacga gaagaacaac
     2701 atcatccacc acaccaccat gatcgagagc gccggccatt acgccttctt ctcggccgac
     2761 aagaagttcg cctcctatga ctgcgaccgc gaggccttca tgggcctcta ccactcggaa
     2821 gccaaccccc aggccgtcct ggcgggcaag tgctcgggct cgaccgccga cggcggcaac
     2881 ccggtcgcgg ccacgaccct ttcggtcacc ctcaagcccg gcgagaccaa gacggtcacc
//
LOCUS       DASTJJ010000514         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1417509, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000514 DASTJJ010000000
VERSION     DASTJJ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417509"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..854
                     /locus_tag="VFR02_06325"
     CDS             285..854
                     /locus_tag="VFR02_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9870091.1"
                     /translation="MAFGAATLFLIIFFGSVLLLFFYGVAVYNRLVKYRVEVDNAWSQ
                     IDVQLKRRHDLIPNLVETVKGVMNFEKDTLTRVMQARAAAMGATNPADRVKAENDLSG
                     GLGRLMAVWENYPDLKSNKNAADLQEELTSTENKIAYSRGYYNDIVANFNALIQQFPS
                     NLVAAPFGFQPREFLKAAETDREVPAVKL"
BASE COUNT          175 a          312 c          313 g          217 t
ORIGIN      
        1 tcttgtgggt ctttttgggg gcggcggtct tcgccttgga tgggggggct tttttcttcg
       61 cggccggctt tttcttggat tgggccatgc tctgcctcct gtttaggtcc gggcggggag
      121 ggggaaggat ggacccggaa aaggatggaa gaataacgaa gcctcccgga cctgtcaaga
      181 attttgaggt ggcaggcgcc cccgggacgg cccttttttg ggggcgtccg gggcgcctgg
      241 ttggtagaat ggcccgacgt cattaaagcc gaccaggatc ccccatggcc tttggagccg
      301 ccaccctgtt cctcatcatc tttttcggtt cggtccttct tctcttcttt tacggcgtcg
      361 cggtctacaa ccgcttggtg aaataccggg tggaagtgga caacgcctgg agccagatcg
      421 acgtgcagtt gaagcgccgg catgacctca tcccgaacct ggtggagacc gtcaagggcg
      481 tgatgaattt cgaaaaggac accctgaccc gggtcatgca ggcccgggcc gcggccatgg
      541 gcgccaccaa cccggcggac cgggtgaagg ctgagaacga cctttcgggc ggcctgggcc
      601 gcttgatggc ggtctgggaa aattacccgg acctgaaatc caacaagaac gccgcggacc
      661 tgcaggagga actgacctcc accgagaaca agatcgccta ttcccgcggc tactacaacg
      721 acatcgtggc caatttcaac gccctcatcc aacagttccc ttccaacctg gtggccgccc
      781 ccttcgggtt ccaaccccgg gaattcctga aggccgccga aacggacagg gaagtccccg
      841 ccgtcaagct gtagaccgtg ttggaatccc ttccggcctc cacggtcttc gaggcgcggg
      901 aacggaaccg gagggccacg gccttcctgt tggcctcctt ggtcctgctt tacgccctct
      961 tcctcaacct gatggtcctg gcggtctgtt tttgggcctg ccggtccggc gttcccc
//
LOCUS       DASTJJ010000515         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1540150, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000515 DASTJJ010000000
VERSION     DASTJJ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540150"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1150
                     /locus_tag="VFR02_06330"
     CDS             <1..>1150
                     /locus_tag="VFR02_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HET9870092.1"
                     /translation="YHDHLKWHVLETDHFELYYYDPCEKLAQTAARDAETAFLKTSRV
                     FNYAPQPQDKIPLFLYATPQQFQETNITQEYLDEGVGGFTEVFKNRIAIPMDGSYFEL
                     NKVITHELTHAFQYDLIYGEGWRSINLFKSVLVPTWMMEGMAEWNARHLDGGGEMVLR
                     DAVLNDQVLSLGLLHSFGHFHQVYTAYKESQSLLDYVTQLYGPDKVPEMMKRMADNQS
                     PGTVIKALLGVSEDQLYDNWHFYMKTRAWARIQGMPAPERYGDSLEEGVGKSAVSPDG
                     RWVAALKRDQLDLIDVPTKNKSGLKGDRFNTRGTGLAWSPDGRFLAYVVDREGENRLV
                     VMRLSDHQTREFPFPRFSMLFSPAWSPDQRYLVFTGYDDESTDLYRFEI"
BASE COUNT          229 a          393 c          331 g          197 t
ORIGIN      
        1 ctaccacgac cacctgaaat ggcacgtgct ggagaccgac catttcgagc tctactatta
       61 cgacccctgt gaaaaactgg cccaaacggc cgcccgggac gccgaaaccg ccttcctcaa
      121 gaccagccgg gtcttcaact acgcgcccca gccccaggac aagatccccc ttttcctcta
      181 cgccacgccc cagcagttcc aggagaccaa catcacccag gaatacctgg acgagggcgt
      241 ggggggcttc accgaggtct tcaagaaccg catcgccatc cccatggacg gttcctattt
      301 cgagctcaac aaggtcatca cccacgagct gacgcacgcc ttccaatacg acctcatcta
      361 cggcgagggc tggcggtcca tcaacctctt caagtccgtg ctggtgccca cctggatgat
      421 ggagggcatg gccgaatgga acgcgcggca cctggacggc ggcggcgaga tggtgctgcg
      481 ggacgccgtg ctcaacgacc aggtcctgtc cctgggcctg ctccattcct tcggccattt
      541 ccaccaggtc tacacggcct ataaggaatc ccagagcctg ctggactacg tgacccagct
      601 ttacggcccc gacaaggtgc ccgagatgat gaaacgcatg gccgacaacc agtccccggg
      661 tacggtcatc aaggcgctct tgggcgtcag cgaggaccag ctttacgaca actggcattt
      721 ctacatgaag acccgggcct gggcccgcat ccagggcatg cccgcccccg aacgatacgg
      781 ggactccctg gaggaggggg tggggaagag cgcggtctcg cccgacggcc ggtgggtggc
      841 cgccctcaag cgggaccagt tggacctgat cgacgtgccg accaagaaca agagcggcct
      901 caagggcgac cgcttcaaca cccggggcac gggactggcc tggtcgcccg acggccgttt
      961 cctggcctat gtggtggacc gggaagggga gaaccggttg gtggtgatgc gcctttccga
     1021 ccaccagacc cgggaattcc cctttccccg attttccatg ctcttttcac cggcctggtc
     1081 gcccgaccag cgttacctgg tcttcacggg ctatgacgac gagtccaccg acctctaccg
     1141 cttcgagatc
//
LOCUS       DASTJJ010000516         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1417583, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000516 DASTJJ010000000
VERSION     DASTJJ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417583"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFR02_06335"
     CDS             complement(<1..448)
                     /locus_tag="VFR02_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870093.1"
                     /translation="MESFYDYKKFYVLYVDDEEKSLKYFRQTFGDKFPILTAPSAEEG
                     LRILKENQDKTAVLMTDQRMPGEKGIQFLEKARQLNPRILRILVTAYSDLETAIEAVN
                     TGAIYKYVTKPWDIPQLENTLRRAVEFFIVQSERDQLLREKMSVLHN"
     gene            complement(481..>1116)
                     /locus_tag="VFR02_06340"
     CDS             complement(481..>1116)
                     /locus_tag="VFR02_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870094.1"
                     /translation="LNYAAQALYVLKTKKDQLPESERADYAEVLADIEDGVQRVKHIV
                     TDLRTFTHPHMGGGRDLVEVKDVVETALRFLSHELKDRVEVTLALPEGLTMQADRNKM
                     VQVFVNLVQNAGDALRDKAPGGEGPRLWIEGREEKGRTLLRVRDNGPGIEPGVIDKIF
                     DPFFTTKDVGQGMGLGLSLCFRIVQEQEGRLSVSSEWGKGAEFTVDLPARA"
BASE COUNT          177 a          371 c          327 g          241 t
ORIGIN      
        1 tgttgtgcag cacggacatc ttttcccgca gcagctggtc gcgctcgctc tgcacgatga
       61 agaattccac ggcgcggcgc agcgtgttct ccagttgggg gatgtcccag ggcttggtga
      121 cgtatttata gatggccccg gtgttgacgg cctcgatggc ggtctccagg tccgaatagg
      181 cggtgaccag gatgcgcagg atgcgggggt tcagctgccg ggccttttcc aggaactgga
      241 tgcccttctc ccccggcatg cgctggtcgg tcatgagcac ggcggtcttg tcctggttct
      301 ccttcaggat ccgcaggccc tcctccgcgc tgggggcggt caggatgggg aacttgtccc
      361 cgaaggtctg ccggaagtac ttcagggact tctcttcgtc gtccacataa agcacgtaaa
      421 attttttgta gtcgtagaac gattccataa atgctctcct tgatggacct gccgacccga
      481 tcaggccctc gcgggcagat cgaccgtgaa ttcagctccc ttgccccatt cactgctcac
      541 cgacaaccgg ccttcctgct cctgcacgat gcggaagcaa aggctcagcc ccagccccat
      601 cccctggccc acgtccttgg tcgtgaaaaa ggggtcgaag atcttgtcga tcacgcccgg
      661 ctcgatgccc gggccgttgt cccgcacccg cagcagcgtc cggcccttct cttcccggcc
      721 ttcgatccaa agccgggggc cttccccgcc gggcgccttg tcccgcaggg cgtccccggc
      781 gttctggacc aggttcacga agacctggac catcttgttg cggtccgcct gcatggtcag
      841 cccctcgggc agggccaggg tgacctcgac ccggtccttg agctcgtggc tcaagaaccg
      901 caaggccgtc tccaccacgt ccttcacttc caccaggtcc cggccgccgc ccatgtgcgg
      961 atgggtgaag gtgcgcaggt ccgtgacgat atgcttgacc cgctgcaccc cgtcctcgat
     1021 gtcggccagc acctcggcgt aatccgcgcg ctccgactcg ggcaattgat ctttttttgt
     1081 ctttagaaca tacagggctt gggccgcata gttaag
//
LOCUS       DASTJJ010000517         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1784567, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000517 DASTJJ010000000
VERSION     DASTJJ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784567"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..>1937)
                     /gene="priA"
                     /locus_tag="VFR02_06345"
     CDS             complement(203..>1937)
                     /gene="priA"
                     /locus_tag="VFR02_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET9870095.1"
                     /translation="ALADALKLAGLGSLRVAGEAGEEARDGGFPLGKAQAAPELHPQQ
                     QAALTPLTAAIQAGGFRAFLLQGVTGSGKTEVYLRAIEETLKKGKQAVVLIPEIALTP
                     QTVERFTGRFGARVAVLHSRLDPAERARQWKRLAAGQAAIAVGARSALFAPVPALGLI
                     VVDEEHEGSYKQEETPRYQARDSAVKRAQLTGAVAILGSATPSLETLKNAQEGKYGWL
                     TLPERVNRRPLPRVRVVDLKEEAQALGGERPVLSLALETALRENLDKGEQSILFLNRR
                     GFNTVTLCLKCGEPVHCPHCAVPVTTHRVGGKVTLTCHYCDWKGPVPAKCPACGGAPV
                     QPLGLGTERLEEELKALFPKARVARMDLDTTAERGSHQKILDRFRAGEADILLGTQMI
                     AKGHDFPGVTLVGVVGADTGLSLPDFRASERVFQLLVQVAGRAGRAEKEGVIYLQTYN
                     PDHPSVKAAARHDTEGFWAAELRLRQELDYPPFTRLGLLVFRSEDEKKAQAAAARAAS
                     FLTGREPGVEARGPAPAAFTKIRGHYRWQVLLKSKNPAALRRLLAAMDAELEPPKGVF
                     RTVDLDPQSLL"
BASE COUNT          280 a          692 c          651 g          314 t
ORIGIN      
        1 caactgatgg gcgtggtcca agtggccttg agccagtggc cgaagaaagc ctgacttcca
       61 tcacaccagt aaaaactaca aacgctgtga acaaggcaga cccgttgccc aaaggcaagt
      121 tcaagctaaa gacccgatcc aatttttcct ttttgcatcc cttggcgtct tagcgtcttt
      181 gcgtgcgcct taaatctcta cttcacaata atgactgcgg gtccaggtcg accgtgcgga
      241 agacgccttt gggcggttcc agttcggcgt ccatggcggc caggagcctg cgcagggcgg
      301 cggggttctt cgacttcaag agcacctgcc agcggtagtg gccccggatc ttggtgaagg
      361 ccgccggggc ggggccccgc gcctccacgc ccggctcccg gcccgtcagg aacgaggccg
      421 cccgggcggc ggccgcctgg gccttcttct cgtcctccga gcggaacacc aacaaaccca
      481 gccgggtgaa gggcggatag tccagctctt ggcgcaggcg cagttcggcg gcccagaaac
      541 cctcggtgtc gtgccgggcc gccgccttga cgctgggatg gtccgggttg taggtctgca
      601 ggtagatgac gccttccttc tcggcccgcc cggcccggcc cgccacctgc accaaaagct
      661 ggaagacccg ctcggaggcg cggaaatccg gcagcgacaa cccggtgtcg gcgcccacca
      721 cgcccaccag ggtgacgccc gggaagtcat ggcccttggc gatcatctgg gtgcccagca
      781 ggatgtcggc ctcccccgcg cggaagcggt ccaggatctt ctggtggctg ccccgctcgg
      841 ccgtggtgtc caggtccatg cgggcgacgc gggccttggg gaaaagggcc ttcagttcct
      901 cctccagccg ctcggtgccc agccccaggg gctgcacggg ggccccgccg caggccgggc
      961 acttggccgg caccggtccc ttccagtcgc aatagtggca ggtcaaggtc accttgcctc
     1021 ccacccggtg ggtggtgacg ggcacggcgc aatgggggca atgcacgggc tcgccgcact
     1081 tgaggcagag cgtgaccgtg ttgaagcccc ggcggttgag gaagaggatc gattgctcgc
     1141 ccttgtccag gttctcccgc agggccgttt ccagcgccag cgacagcacg ggccgctcgc
     1201 cgcccagggc ctgggcctct tccttcaggt ccaccacccg caccctcggc aggggccgcc
     1261 ggttgacccg ctcgggcagg gtgagccagc cgtacttgcc ttcctgggcg ttcttcaggg
     1321 tctccagcga gggcgtggcg ctgcccagga tggccacggc gcccgtcaac tgggcccgct
     1381 tcaccgccga atccctcgcc tggtagcggg gcgtctcttc ctgcttgtag gacccctcgt
     1441 gttcctcatc caccacgatc aggcccaggg ccggcacggg ggcgaagagc gccgaacggg
     1501 cgcccaccgc gatggcggcc tggcccgcgg cgaggcgttt ccattgccgg gcccgctcgg
     1561 ccgggtccag gcggctgtgc agcacggcca cccgggcccc gaagcggccc gtgaagcgtt
     1621 ccacggtctg gggggtgagg gcgatctcgg ggatgagcac cacggcctgc ttcccctttt
     1681 tcagcgtctc ctcgatggcc ctcaggtaga cctcggtctt gcccgagccg gtcacgccct
     1741 gcagcaggaa ggcccggaag ccgcccgcct ggatggccgc cgtcaagggc gtgagggcgg
     1801 cctgctgttg ggggtgcagc tcgggcgcgg cctgggcctt ccccaagggg aagcccccgt
     1861 cccgcgcctc ctcccccgct tccccggcca cccgcaggct ccccaggccc gccagcttca
     1921 gggcgtcggc cagggcc
//
LOCUS       DASTJJ010000518         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_683614, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000518 DASTJJ010000000
VERSION     DASTJJ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683614"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFR02_06350"
     CDS             complement(<1..260)
                     /locus_tag="VFR02_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870096.1"
                     /translation="MSLFLRILGIGLLLAMVYALVVGLGLTRSFKHHKQAKKILVLND
                     FEYPDTDLDWATGGYVKVESSTENITHGKRSLQAEYLLPSQF"
     gene            complement(395..1117)
                     /locus_tag="VFR02_06355"
     CDS             complement(395..1117)
                     /locus_tag="VFR02_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9870097.1"
                     /translation="MGSLVLYCVLVLLTAWAGAAAPFLFHRRKDGHPFLHFFISFGAG
                     IMLGAAFLHLLPDAFRLAGEDAGFAALAGFLLLFLLESFSFSHPCEDEQHDYHALGWV
                     AVLGLSLHNLVNGVALGAGARVPVVGWVVFAATVAHKAPEFFSLSSILLAGRRPVWRT
                     AALVLTVSAMIPLGAFASNLFLSQSGERVLGLALGFSSGTFIHLAVVDLAPEMRQHRD
                     RLAHFASFLLGVALMALAAWLE"
     gene            complement(1215..1628)
                     /locus_tag="VFR02_06360"
     CDS             complement(1215..1628)
                     /locus_tag="VFR02_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9870098.1"
                     /translation="MSFGQLSRRHFGDLLSEGNFWDWAEGRTQILPAVDVEETQNEYI
                     FRAELPGVDKDQVKIALADNVLTISGEKRSEERKSDRTYHRVERLAGSFRRTYSLSQP
                     LQADKVSARYKDGVLEITVPKAEEAKPREIQIQVQ"
     gene            1765..>2446
                     /locus_tag="VFR02_06365"
     CDS             1765..>2446
                     /locus_tag="VFR02_06365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9870099.1"
                     /translation="MSATLAAAVAAAKAAGKVHRTRYGRVRDIRFKSGNQLNLVTEVD
                     GRCERLIRGLLTRRFPTHAFWGEEGGGALDAKRPTWIVDPLDGTTNYAHGYPFFCVSI
                     ALVREGRLAAGVIYDSLRDECFTAERGKGAFLNGRRLRVSRVGAIGQSLLCTGFAYGV
                     LQTRYNFDNFRNFVMKSQGVRRDGSAAMNLAYVAAGRFEGFWERGIQAWDMAAGVLLV
                     REAGGRVTD"
BASE COUNT          458 a          826 c          791 g          371 t
ORIGIN      
        1 atgaattggg aaggcagcag gtattcggcc tgcagggatc gtttgccgtg ggtgatgttc
       61 tcggtggagc tttccacctt cacgtagccg cccgtggccc agtccaggtc cgtgtcgggg
      121 tactcaaaat cgttcaagac caggatcttc ttggcctgtt tgtggtgttt gaagctgcgg
      181 gtcaacccca ggcccaccac cagggcgtag accatggcca agagcagtcc gatccccaag
      241 atccttaaaa acaagctcat tcccacatcc tggcctggaa tttcccgacg gaggcgaaac
      301 cggggcattg tatcagccgc cccaggctcc tgcaacttgg aaacgtttta aggccggaac
      361 cgcccgcaac gaggcccatt ccaccggggg gatctcattc cagccaggcg gccagcgcca
      421 tcagggccac cccgagcagg aacgaggcga aatgggccag gcggtcgcgg tgctggcgca
      481 tctcgggcgc caggtcgacc accgccaagt ggatgaaggt gcccgaggaa aagcccaagg
      541 ccaagcccag gaccctttcg ccgctttggg acaagaacag gttggaggcg aaggccccca
      601 gggggatcat ggccgaaacg gtcaacacca gggccgccgt gcgccaaacg gggcgccggc
      661 ccgccaagag gatgctggag agggaaaaga actcgggcgc cttgtgcgcc accgtggccg
      721 cgaacaccac ccaacccacc accggcaccc gcgcgcccgc gcccagggcc acgccgttca
      781 ccaggttgtg caggctcagc cccagcaccg ccacccaacc cagggcgtgg tagtcgtgct
      841 gttcgtcctc acagggatgg gaaaagctga aggactccag gaggaacagc aacaggaacc
      901 cggccagggc ggcgaacccc gcgtcctccc ccgccaagcg gaaggcgtcg ggcaacaggt
      961 gcaggaaggc ggcccccagc atgatgccgg cgccgaaact gatgaaaaaa tgcaggaaag
     1021 gatggccgtc tttgcgccgg tggaacagga acggcgccgc cgcgcccgcc caggcggtca
     1081 agagcaccag gacgcaataa agaaccaagg aacccaccgc cgcacccccg ccccacgatg
     1141 ggccccaggt tacacgaagt gcgcggggga ccggaaataa aaaaggcccc cggttccccg
     1201 ggggcccgtc ccgctcactg cacctggatc tggatctccc gcggcttggc ctcctcggcc
     1261 ttgggaacgg tgatctccag cacgccgtcc ttgtaccgcg ccgagacctt gtcggcctgg
     1321 aggggttggg acagcgaata ggtccggcgg aaggacccgg ccaagcgttc cacccggtgg
     1381 taggtccggt cggacttcct ttcctcggac cgtttctcgc ccgagatcgt cagcacgttg
     1441 tcggccagtg cgatcttgac ctggtccttg tccaccccgg gcagctcggc ccggaaaata
     1501 tactcgttct gcgtctcctc cacgtccacc gcgggcagga tctgggtgcg gccctcggcc
     1561 caatcccaga aattcccctc cgacagcagg tcgccgaaat gccggcgcga caattgacca
     1621 aatgacatga cgtcctcctt gaagctcccc caggggggtt gtgatgttcc tgaccccatc
     1681 agtaggaagg cgcccggacg gcgtcaaggc ccaccgccgg gccttcttct gctagaatcg
     1741 gccgatccca ctgaaaggca ccccatgagc gcaaccctcg ccgccgccgt cgccgccgcc
     1801 aaagccgccg gaaaggtcca ccggacccgg tatggccggg tgcgcgacat ccggttcaag
     1861 tcgggcaacc agttgaacct ggtcaccgag gtggacggcc gctgcgaacg gctcatccgc
     1921 ggcctgctga cccggcgctt ccccacccac gccttttggg gcgaggaggg cggcggggcc
     1981 ctggacgcca agcgccccac ctggatcgtg gaccccttgg acggcaccac caattacgcc
     2041 catgggtacc ccttcttttg cgtgtccatc gccctggtgc gggaggggcg cctggccgcg
     2101 ggggtcattt acgactccct gcgtgacgag tgcttcaccg ccgaaagggg caaaggcgcc
     2161 ttcctgaacg gccggcgcct ccgggtgtcg cgggtgggcg cgatcggcca aagcctgctc
     2221 tgcacgggct tcgcctacgg ggtgctccag acccggtaca atttcgacaa cttccggaat
     2281 ttcgtgatga agtcccaggg cgtgcgccgg gacgggtcgg ccgccatgaa cctggcctac
     2341 gtggccgcgg gccgcttcga gggcttttgg gagaggggca tccaggcctg ggacatggcc
     2401 gcgggggtcc tgctggtgcg ggaggccggg gggcgggtca cggaca
//
LOCUS       DASTJJ010000519         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_929848, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000519 DASTJJ010000000
VERSION     DASTJJ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929848"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFR02_06370"
     CDS             complement(<1..1539)
                     /locus_tag="VFR02_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET9870100.1"
                     /translation="MTVFLFSEIDHPPLFEERGTPETENLLAAQYFLLEEILHGHGAS
                     WLKRTPRGVLASYEGGRPLEAALEAMARFQERAWGRWGRLALKAALHAGEADLWDQDC
                     VGPDVQHTLKLLDAAWGGQVLLTPALLAARPLPDGASTRDLGTHLLKDLARPQSVHLL
                     RHPSLPDKEFPPLKSLAAYPNNFTFQPTPFFGREEEARQIEEQLLQEGTRLVTLAGPG
                     GFGKTRLALQTAADLVDHFPQGAFLVPLAPLSSDQFIVEKIAGVLHFAFSGAEDPKRQ
                     LFDFLRDKNLLLVMDNFEHILEGARLITEILEAAPSVKVLVTTRERLRLPAEKVLEIR
                     GLRCPAEDDPHWEAASAVQLFLKSLHKQEPAFRAGLGDRPAVVRICRILEGMPLGIEL
                     ASTWIKTLPLSEIPDKIQSNRETVALVMPHLPSRHQSLRAVFEYSWVLLTPAQKQVLR
                     TASVFRGGFSMDAAVRIAGASPATLKQLEDKFLVRHKPHERFELHELLKYYAKEKLFD
                     SPKEK"
BASE COUNT          265 a          539 c          575 g          287 t
ORIGIN      
        1 cttctccttc gggctgtcga acagtttttc cttggcgtag tacttgagca gttcgtgcag
       61 ctcaaagcgt tcatggggct tatgccgcac gaggaacttg tcctccaact gcttcagggt
      121 ggccgggctg gcccccgcga tgcgcacggc cgcgtccatc gagaagcccc cgcggaacac
      181 cgaggccgtg cgcagcacct gcttttgggc cggggtcagg agcacccagg aatactcgaa
      241 caccgcccgc aggctctggt ggcggctggg caggtggggc atgaccaggg ccaccgtctc
      301 ccggttgctt tggatcttgt cggggatctc ggagagtggc agggtcttga tccaggtgga
      361 ggccagttcg atgcccaggg gcatgccctc caggatccgg cagatgcgca ccacggccgg
      421 ccggtccccc aggcccgccc ggaaggccgg ctcctgcttg tgcaggctct tgaggaacag
      481 ctgcaccgcg ctggcggcct cccaatgggg gtcgtcctcc gcgggacagc gcaggccccg
      541 gatctccaat accttttcag cgggcaggcg cagccgctcg cgcgtcgtca ccagcacctt
      601 gaccgagggg gccgcctcca ggatctcggt gatgagccgg gccccttcca ggatgtgctc
      661 gaagttgtcc atgaccaaca gcaggttctt gtcccgcagg aagtcgaaga gctggcgttt
      721 ggggtcctcc gccccgctga aggcgaaatg cagcaccccg gcgatctttt ccacgatgaa
      781 ctggtccgag ctcaaggggg ccaggggcac caggaaggcg ccctggggga aatggtccac
      841 caggtccgcg gcggtctgga gggccaaccg ggtcttgccg aacccccccg gcccggccag
      901 ggtcaccaat cgggtgccct cctgcagcaa ttgttcctcg atctgccggg cttcttcctc
      961 gcgcccgaag aagggcgtgg gctggaaggt gaaattgttg ggataggccg ccaacgactt
     1021 cagcgggggg aattccttgt cgggcagcga gggatgccgc agcaggtgga cggactgggg
     1081 gcgggccagg tccttcaaca agtgggtacc caggtcgcgc gtggaggcgc cgtcgggcag
     1141 gggccgggcc gccaacaggg cgggcgtcag gagcacctgc cccccccagg ccgcgtccag
     1201 cagcttcagg gtgtgctgca catcggggcc cacgcagtcc tggtcccaaa ggtcggcttc
     1261 cccggcgtgc agggcggcct tgagggccaa gcggccccat cggccccagg cgcgttcctg
     1321 gaaccgggcc atggcttcca gggcggcctc caggggccgc ccgccttcgt aggaggccag
     1381 cacgccccgg ggcgtgcgct tgagccagga cgcgccgtgg ccatgcagga tctcctccaa
     1441 caggaaatac tgggccgcga gcaggttctc ggtctcgggg gttccccttt cttcaaaaag
     1501 ggggggatga tcgatctcgg aaaaaaggaa caccgtcatg tcggctccaa ggcgtggcgg
     1561 atcgtccggg tgtccagggt cttcggtttt tcccactata gcaccggggc ggccaaaatc
     1621 ctcctttaga cttgcgggcc cgcctgttaa acttgccggg tttgcg
//
LOCUS       DASTJJ010000520         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1784605, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000520 DASTJJ010000000
VERSION     DASTJJ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784605"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1732
                     /locus_tag="VFR02_06375"
     CDS             <1..>1732
                     /locus_tag="VFR02_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870101.1"
                     /translation="GNLSREAAHYPVFVDTTPPKSRVNPLPLFLDRTQVPVSWEASDA
                     GSDVACFDVQVKAGPNGAWTDWLTETYERNAVYPGQDGQRYAFRCRAKDKVGNLEPYP
                     SVEMASVTLDISPPPPVTSLRAFSRPKGDIELVWDPVADRVSGTDYYRVYRWVEGEKK
                     QKISQDGAVKEPRYLDQGGGLRENTAYYYCAQAVDKMGNEQHEDNQAAAALSDQGVDP
                     PEISSPTHPPDEWSANHAPILAWKAPSDATGIAGYLYQLDGDPSTLPEPGQALATEDT
                     RASLSELKSGIWYFHLLARDRAGNVSEPVHYRLKIDSEKPAAPDLVSTSHPDETRWYQ
                     AGKVHFQMAPRPKLSGFEAFYYVFDRSPKTLPDPATAIRTTEPSFSAMATEPGVWYLH
                     VVCRDRAGNVGEPAHLAVRTALNQIPPPVVDSPTHPKEEEATTAYSPVFRLTPNTDVK
                     YEAVGWLYKLSRQEEDKLTPDDAFTSENTVEFKDLAEGTWYFHVAAVDAKGRPAPLAA
                     RRKIVIRKLSQLTGRFFRKDAATPVSGTKVELARGEQVVATTLTDPKGAFSFTHMPEG
                     KYEIRLHSDQ"
BASE COUNT          314 a          643 c          548 g          227 t
ORIGIN      
        1 tgggcaacct gagccgcgag gccgcccatt acccggtctt cgtcgacacc acgccgccca
       61 agtcccgcgt gaaccccttg ccgctgttcc tcgaccgcac ccaggtgccg gtgtcctggg
      121 aggcctcgga cgcgggcagc gacgtggcct gcttcgacgt gcaggtgaag gcgggcccca
      181 acggcgcctg gaccgactgg ctgaccgaga cctacgaacg caacgccgtc tacccgggcc
      241 aggacggcca gcgctacgcc ttccgttgcc gggccaagga caaggtgggc aacctggaac
      301 cctatccctc ggtggagatg gcctcggtga ccctggacat ctcgcccccg ccgcccgtga
      361 cgtcgctgcg ggccttctcc cgccccaagg gcgacatcga gctggtctgg gacccggtgg
      421 ccgaccgggt gtcgggcacg gactattacc gcgtctaccg ctgggtcgag ggcgagaaga
      481 aacagaagat ctcccaggac ggggccgtga aggaaccccg ctacctggac cagggcgggg
      541 gactgcgcga gaacacggcc tactattatt gcgcgcaggc cgtggacaag atgggcaacg
      601 aacagcacga ggacaaccag gccgccgccg ccctgtcgga ccagggcgtg gacccgccgg
      661 agatttccag ccccacccac ccgccggacg agtggagcgc caaccacgcg cccatcctgg
      721 cctggaaggc gccctcggac gccaccggca tcgcgggcta cctgtaccag ttggacgggg
      781 acccctccac cctgccggag ccgggccagg ccctggccac cgaggacacc cgcgcttcct
      841 tgagcgaact caagagcggc atctggtatt tccacctctt ggcccgggac cgggccggca
      901 acgtcagcga accggtccat taccggctca agatcgactc ggaaaaaccg gcggcgcccg
      961 acctggtctc caccagccac ccggacgaga cccgctggta ccaggcgggg aaggtgcatt
     1021 tccagatggc gccccggccc aagctgtcgg gcttcgaggc cttctactat gtcttcgacc
     1081 gttcccccaa gaccctgccc gacccggcca cggccatccg caccaccgaa cccagcttca
     1141 gcgccatggc caccgagccc ggggtgtggt acctgcacgt ggtctgccgg gaccgggcgg
     1201 gcaacgtggg cgaaccggcc cacctggcgg tgcgcacggc cctgaaccag atcccgccgc
     1261 cggtggtcga cagccccacc catcccaagg aagaggaagc caccacggcc tacagcccgg
     1321 tcttccgcct gacgcccaac accgatgtga agtacgaggc ggtgggctgg ctctacaagc
     1381 tcagccggca ggaagaggac aagctcaccc cggacgacgc cttcacgtcg gagaacacgg
     1441 tggaattcaa ggacctggcc gagggcacct ggtatttcca cgtggccgcg gtggacgcca
     1501 agggccgccc cgcccccctg gccgcccggc gcaagatcgt catccgcaag ctcagccagc
     1561 tgaccggccg ctttttccgc aaggacgccg ccacgccggt gtcgggcacc aaggtcgaac
     1621 tggcccgggg cgagcaggtg gtggccacca cgctcaccga ccccaagggc gccttttcct
     1681 tcacccacat gcccgagggc aagtacgaga tccgcctgca tagcgaccag ta
//
LOCUS       DASTJJ010000521         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1295171, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000521 DASTJJ010000000
VERSION     DASTJJ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295171"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1173)
                     /locus_tag="VFR02_06380"
     CDS             complement(<1..>1173)
                     /locus_tag="VFR02_06380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484187.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET9870102.1"
                     /translation="WGHDMGYFASDDDAEVFHMELMHLLVHQKMAFNSPGWFNVGIEA
                     RPQCSACFINSVSDSMESILDLAKTEGMLFKYGSGTGTNFSALRSSKEKLSGGGTASG
                     PVSFLKGFDSFAGVIKSGGRTRRAAKMVILDADHPDIVEFIKCKSDEEKKAWALIEAG
                     YDGGFNVPGGAYDSVQFQNANHSVRVSDEFMQAAAAGGTWTTKSRKDGSPMDSYSAKD
                     LLRMIAESTWICGDPGMQYDTTINKWHTSANTARINSSNPCSEYMYLDDSACNLASLN
                     LMKFRTAEGDFDIEAFRHAVDITILGQEITVDNASYPTKPIERNSHDYRPLGLGYANL
                     GALLMSRGLPYDGDEGRNYAAAITALMQGEANLMSSKIAKEVGPFRGYARNREPMLK"
BASE COUNT          178 a          355 c          411 g          229 t
ORIGIN      
        1 cttcagcatg ggctcgcggt tgcgggcata accccggaag gggcccactt ccttggcgat
       61 cttggaggac atcaggttgg cctcgccctg catgagggcg gtgatggcgg cggcgtagtt
      121 gcggccctcg tcgccgtcat agggcagccc gcggctcatg agcagcgcgc ccaggttggc
      181 gtagcccagc cccaggggcc ggtagtcgtg gctgttgcgc tcgatgggct tggtggggta
      241 gctggcgttg tccaccgtga tctcctggcc caggatggtg atgtccaccg cgtggcggaa
      301 ggcctcgatg tcgaagtccc cttcggcggt gcggaacttc atcaggttca aggacgccag
      361 gttgcaggcc gaatcgtcca ggtacatata ctcggagcag gggttggacg agttgatgcg
      421 ggccgtgttg gcgctggtgt gccacttgtt gatggtggtg tcgtactgca tgccggggtc
      481 gccgcagatc caggtggatt cggcgatcat gcgcagcagg tccttggcgc tgtagctgtc
      541 catgggcgac ccgtccttgc gggacttggt ggtccaggtg ccgcccgcgg cggccgcctg
      601 catgaactca tcggacacgc gcaccgagtg gttggcgttc tggaactgca ccgagtcgta
      661 ggcgccgccc ggcacgttga agccgccgtc gtagccggcc tcgatcaggg cccaggcctt
      721 cttctcctcg tcggacttgc acttgatgaa ttccacgatg tccggatggt cggcgtccaa
      781 gatgaccatc ttggccgcgc ggcgcgtgcg gccgcccgac ttgatgacgc ccgcgaagct
      841 gtcgaagccc ttgaggaacg acaccgggcc cgaggccgtg ccgccgcccg agagcttttc
      901 cttggacgag cgcagggccg agaagttggt gcccgtgccc gagccgtact tgaaaagcat
      961 gccctcggtc ttggccaggt cgaggatcga ttccatgctg tcggacacgc tgttgatgaa
     1021 gcaggccgag cactgggggc gcgcctcgat gcccacgttg aaccacccgg gcgagttgaa
     1081 ggccatcttc tggtgcacca agaggtgcat cagctccatg tggaagacct cggcgtcgtc
     1141 gtccgaggcg aagtagccca tgtcgtggcc cca
//
LOCUS       DASTJJ010000522         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_805322, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000522 DASTJJ010000000
VERSION     DASTJJ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805322"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..384)
                     /locus_tag="VFR02_06385"
     CDS             complement(61..384)
                     /locus_tag="VFR02_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9870103.1"
                     /translation="MANSKTAVIFQALGDPTRREIFERLVKGPQAVVDLSAGLHVSRP
                     AVSQHLKVLKDSGLVLLETRGTRHLYRVDPEGVTAMRDYLDRLWDAALTAFKAAAEKA
                     QKGEK"
     gene            525..>1235
                     /gene="ygiD"
                     /locus_tag="VFR02_06390"
     CDS             525..>1235
                     /gene="ygiD"
                     /locus_tag="VFR02_06390"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HET9870104.1"
                     /translation="MDKMPVLFVGHGSPMIGVEDNPFTKSLGRLGRELPRPRAVAVVS
                     AHWVTPGTRVGTQPAPKQIYDFGGFPPELYAVKYPAPGAPGEAKRLVQELGLAADESW
                     GLDHGAWTVLKHLYPQAEVPVFQVSLDRGLDFEGHLALGRKLGVLRERGLLLLGSGNL
                     THNLRDLDFHEDAKPFDWALDFDAKAKAALEARDPQAFTHPGRTFGESAFRKAHPTAE
                     HYLPLLYALGASAPGDQLS"
BASE COUNT          211 a          405 c          379 g          240 t
ORIGIN      
        1 cggtcttttg ggaggcttgg accaccagct ctttcttgat cgaccgcttc gttccgccgc
       61 tcatttttca cctttctggg ccttctcggc cgcggccttg aaggcggtca aagccgcgtc
      121 ccaaagcctg tccaaatagt cccgcatggc cgtcaccccc tcggggtcca cccggtaaag
      181 atggcgggtc ccccgggtct ccagcaagac cagcccggag tccttcagca ccttgaggtg
      241 ctgggagacc gccggccgcg agacgtgaag gccggcgctc aaatccacga cggcttgggg
      301 ccccttgacg aggcgctcga agatctcgcg ccgggtggga tcccccaagg cctggaatat
      361 caccgctgtt tttgagttag ccataactta cggtaagtaa tagcttactc ttttaaaaat
      421 gtcaaccggt ctttttatcc ggatcctcct gggcgtttcc cgccggtaac ttcttgtaac
      481 taaggcacct tgttcccatg atggtcccaa gaaacgaggc cgccatggac aagatgccgg
      541 tcctcttcgt cggccatggg tcgcccatga tcggggtgga ggacaaccct ttcacgaaaa
      601 gcctggggcg cctgggacgg gaactgcccc gcccccgggc cgtcgccgtg gtgtcggccc
      661 attgggtcac gccgggcacg cgcgtgggca cccagcccgc gcccaaacag atctacgact
      721 tcggcgggtt cccgcccgaa ctttacgccg tgaagtatcc ggctcccggc gcgcccgggg
      781 aagccaagcg gctggtccag gaactgggtt tggcggccga cgaaagttgg ggattggacc
      841 acggggcttg gaccgtcctg aaacatcttt atccccaagc cgaggttccc gttttccaag
      901 tgagcctgga ccggggcctt gatttcgagg ggcacttggc gttaggccgg aagttgggcg
      961 tcttgcggga gcggggcctg ctgctcttgg gcagcggcaa cctgacccac aatttgaggg
     1021 acctggactt ccacgaggac gccaagccct ttgattgggc gctggatttc gacgccaagg
     1081 ccaaggccgc cttggaagcg cgcgaccccc aagccttcac ccacccgggc cggaccttcg
     1141 gggaaagcgc cttccgcaag gcgcacccga ccgccgaaca ttacctgccc ctgctctacg
     1201 cgttgggggc ctcggcgccg ggggaccagc tgtcc
//
LOCUS       DASTJJ010000523         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_684004, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000523 DASTJJ010000000
VERSION     DASTJJ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684004"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFR02_06395"
     CDS             <1..395
                     /locus_tag="VFR02_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9870105.1"
                     /translation="VLGRGAMEGLAVFNGQNWESEVLLAEGPVLVDFRAPWCDPGVFR
                     NGELEDFCGSWKNFVRVGWVDVLRFKESVFRYHLQEFPTLVLFQSGHWVKAFGGPSRV
                     EDLRDWLLTPEALEEEDPAFHVPRGARA"
     gene            392..559
                     /locus_tag="VFR02_06400"
     CDS             392..559
                     /locus_tag="VFR02_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870106.1"
                     /translation="MIRKLPGGRYRLYASKIDPRTGRRRNLGTFRTRAGAQKHEREIQ
                     FFKRQTRPRRG"
     gene            complement(582..833)
                     /locus_tag="VFR02_06405"
     CDS             complement(582..833)
                     /locus_tag="VFR02_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870107.1"
                     /translation="MAEMKDRQKIIDIFLEKQHDQWRAKAYERLCRDHACQLRSDYEL
                     WVHNADQKKPPKPYRNERYPENPGMRFRHSGTDRPWDKR"
     gene            954..>1865
                     /locus_tag="VFR02_06410"
     CDS             954..>1865
                     /locus_tag="VFR02_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9870108.1"
                     /translation="MGRDGRWVKWFGEIGMGDLPQVGGKNASLGEMTRHLVKEGVRVP
                     GGFAVTAAAYRQLLLENKLEGPLQRLTGEAAAGRAARRLLLSAKIPAPVEAAVRRAYR
                     ELCRREGDPRLAVAVRSSATAEDLPDASFAGQQETFLNVRGEEALARACRRCYASLYT
                     DRAISYRRDKGFADKRVDLSIGVQRMVRSDLGASGVMFSIDTESGFPRVVLINAAFGL
                     GETVVQGEVDPDEYMVFKPLLGRKGLVPILEKKKGAKDLKLVYRPGGQGTRLARTTPR
                     ERASFVLTDAEILQLARWAVQVERHYGK"
BASE COUNT          293 a          580 c          668 g          324 t
ORIGIN      
        1 aggttctagg gaggggcgcc atggaggggt tggctgtttt caacgggcag aactgggagt
       61 cggaagtgct cttggccgag gggccggtat tggtcgattt ccgggccccc tggtgcgacc
      121 ccggggtctt ccgcaatggg gaactggagg acttttgcgg atcgtggaag aacttcgtgc
      181 gggtcggctg ggtggatgtg ctgcggttca aggagtcggt cttccggtac cacctgcagg
      241 agttccccac cctggtgctt ttccaatcgg gccattgggt caaggccttc ggcgggcctt
      301 cccgggtgga ggaccttcgg gactggctct tgacccccga ggccttggaa gaggaagacc
      361 ccgccttcca cgtcccgcgc ggggcccggg catgatccgc aaactgcccg ggggccgtta
      421 ccggctttat gcctccaaga tcgacccccg gaccggccgg cgccgcaacc tgggcacctt
      481 ccggacccgc gcgggggccc aaaagcacga gcgggagatc caattcttca agcggcaaac
      541 ccgaccccga cggggttagg cccggggcgg catgccgcct ttcaacgctt gtcccaaggc
      601 cggtcggtcc cgctgtggcg gaaccgcatg ccggggttct cgggatagcg ctcgttgcgg
      661 tagggcttgg ggggcttctt ttggtcggca ttgtggaccc aaagttcgta gtccgaacgc
      721 agttggcagg catggtcccg gcaaagccgc tcataggcct tggcgcgcca ttgatcgtgc
      781 tgtttttcca gaaagatgtc gatgatcttt tgccggtcct tcatttcagc cacctccctt
      841 ttccttcgat cccaggttac cgccgcccgc cgaaaggcct tatgacgccg gtcataagcg
      901 tcccggccga aggggggcat gctggagtcg ggaagaagtc aaaggaggcg gccatgggac
      961 gggacggcag gtgggtgaaa tggttcgggg agatcggcat gggggacctg ccccaggtgg
     1021 gcggcaagaa cgcctccttg ggggaaatga cccgtcactt ggtgaaggaa ggcgtgcgcg
     1081 tgccgggggg gttcgccgtg acggcggcgg cctaccggca attgttgctt gagaacaaac
     1141 tggaaggccc cctgcaacgg ttgaccggcg aggccgccgc gggccgggcc gcccgccggc
     1201 tgctcctctc ggccaagatc cccgcgccgg tggaagcggc cgtccgccgg gcctaccggg
     1261 agctctgccg gcgcgaaggc gacccgcgcc tggcggtggc ggtgcggtcc tcggccaccg
     1321 ccgaggacct gccggatgcc agcttcgcgg gccagcagga gaccttcctg aatgtccggg
     1381 gcgaggaggc cttggcgcgg gcctgccgcc gctgctatgc ctcgctctat accgaccggg
     1441 ccatcagcta ccggcgggac aaggggttcg cggacaagcg ggtggacctt tccatcggcg
     1501 tccagcgcat ggtgcgctcg gacctggggg cgtcgggcgt catgttctcc atcgacaccg
     1561 agagcgggtt cccccgggtg gtcctcatca acgcggcctt cgggctgggg gagaccgtgg
     1621 tccagggcga ggtggacccg gatgagtaca tggtcttcaa acccctcctg ggccggaagg
     1681 gactggtccc catcctggaa aaaaagaagg gggccaagga cctcaaactg gtctaccggc
     1741 ccggcgggca aggcacccgg ctggcccgca ccacgccccg ggaaagggcg tccttcgtcc
     1801 tgaccgacgc cgagatcctc caattggccc gttgggccgt gcaggtggag cgccattacg
     1861 gcaag
//
LOCUS       DASTJJ010000524         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_196352, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000524 DASTJJ010000000
VERSION     DASTJJ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196352"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFR02_06415"
     CDS             complement(<1..535)
                     /locus_tag="VFR02_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870109.1"
                     /translation="MGKPLWLLLVLAVGTLGPAWGGEPFSIPLYDPVSGRDLPGPGAS
                     TPTPTPTVTASQPVPAVTPVPTPVSPGTQPLLRAPDWTEVKTAPGNLHPLGKKSPWMS
                     LLGLGADIPLGTGMARGYAVGFDLDLGTGYRLDDDWSLWANASLGQFSSRDDGLTRHN
                     NFMMIEGAVWAKYRFSRG"
     gene            complement(545..>1584)
                     /locus_tag="VFR02_06420"
     CDS             complement(545..>1584)
                     /locus_tag="VFR02_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9870110.1"
                     /translation="EGAFGLSSGLGYEPGEWCQPAELEALASVLHRYPHAAYYTHIRN
                     YRSNVLDAIGEAIELGRVDQVPVVIQHLLFKLPSNWDQTESGLRLLEEARAQGQEVFA
                     SVYPYDFWGNEVQIPLGQFLYFGPEAYRWSSYTSPDKVTEALAQIHRRLMQYGGGDKV
                     EVTRTGFRKEADWIGLSLADLARKRGMGVDETVLALFLENGPALRICYHGLSEEGLIK
                     NIQSPYVLFGTDSTGNIPHPRDVGAFPRLLGTYVRDKRVVRLSEAIERITREPARLMG
                     LKDRGRLKKGDWGDAVVFNLKTIADKASPSSTLVPPVGIDAVVVNGQVVFQNGVLTGR
                     VPGKVLRRSDQ"
BASE COUNT          273 a          544 c          516 g          251 t
ORIGIN      
        1 cgccccggga gaagcggtac ttggcccaga ccgcgccctc gatcatcatg aagttgttgt
       61 gccgggtgag accgtcgtca cgggagctga actgccccaa ggacgcgttc gcccacaggg
      121 accagtcgtc gtccaggcga tagcccgtcc ccaggtccag gtcaaacccc actgcgtaac
      181 cccgcgccat gcccgtcccc aaggggatgt cggcgcccag ccccagcagg gacatccagg
      241 gtgacttctt ccccagggga tgaaggttcc cgggggcggt cttcacttcg gtccaatcgg
      301 gggcccgcag gagcggctgg gtcccggggg acaccggggt ggggacgggc gtcaccgccg
      361 ggaccggctg ggaggccgtg acggtcggcg tgggggtggg ggtggacgcg ccggggcccg
      421 gcaggtcccg tcccgaaacg gggtcataaa gcgggatcga aaaaggttcc ccgccccacg
      481 cggggccaag cgtcccgacc gccaaaacca agagcaacca cagcggtttt cccatccccg
      541 ccccctattg atcgctgcga cgcagcacct tgccgggcac ccggcccgtc aggacgccgt
      601 tctggaacac cacctggccg ttgaccacca ccgcgtcgat ccccacgggc gggaccagcg
      661 tcgaggaggg gctggccttg tcggcgatgg tcttgaggtt gaagaccacg gcgtcccccc
      721 aatcaccttt cttcaaccgc ccccggtcct tcaaacccat caaccgggcg ggttcgcggg
      781 tgatccgctc gatggcctcc gaaagccgga cgacccgttt gtcgcgcacg taggtgccca
      841 acagccgggg aaaggcgccc acgtcccggg gatgggggat attgcccgtg gagtccgtgc
      901 cgaacaggac gtaaggcgac tggatattct tgatcaaccc ttcctccgaa agcccatggt
      961 agcagatccg aagggcgggc ccgttctcca ggaacagggc caggaccgtc tcgtccaccc
     1021 ccatgccgcg cttgcgggcc aggtcggcca gggaaagccc gatccaatcc gcttccttgc
     1081 ggaaccccgt gcgtgtgacc tcgaccttgt ccccgccgcc atactgcatc aaccgccggt
     1141 ggatctgggc cagggcctcc gtgaccttgt ccgggctcgt gtagctcgac cagcggtagg
     1201 cttccggccc gaaataaagg aattggccca aggggatctg gacctcattg ccccaaaagt
     1261 cgtaggggta cacgctggcg aagacttcct gcccctgggc ccgggcctcc tccagcagcc
     1321 gaaggccgct ttcggtctgg tcccagttgc tgggcagctt gaacagcagg tgctggatga
     1381 ccaccggcac ctggtccacc cggcccagct cgatggcttc cccgatggcg tcgagcacgt
     1441 tggaacggta gttgcggatg tgggtgtaat aggccgcgtg gggatagcgg tgcagcacgg
     1501 aggccaaggc ctccagttcg gccggttggc accattcccc cggctcgtaa cccaagccgc
     1561 tggaaagccc gaaggcccct tcct
//
LOCUS       DASTJJ010000525         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_318641, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000525 DASTJJ010000000
VERSION     DASTJJ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318641"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFR02_06425"
     CDS             complement(<1..345)
                     /locus_tag="VFR02_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9870111.1"
                     /translation="MTAKTEPPHETKPASPELLSRRAFLERLTFILGGIGTLAMSIPL
                     VGYVLGPVLKSDVPKWRRVGKIDDFKVNHTVAVSFDNAAPLAWDGPTGKTGAWLRRNS
                     ETEFIAFALTCTH"
     gene            complement(329..562)
                     /locus_tag="VFR02_06430"
     CDS             complement(329..562)
                     /locus_tag="VFR02_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870112.1"
                     /translation="MSNETHQHDAPPRPAGWHDPKPLKLPPPTVWPTVIALAVVSMAW
                     GAVTSIYITLGGLIAFVVSIIGWIGELRNDRKN"
     gene            complement(572..>1234)
                     /locus_tag="VFR02_06435"
     CDS             complement(572..>1234)
                     /locus_tag="VFR02_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9870113.1"
                     /translation="AEGYLFTAHMFQHMALLMLVPLLFLLGFPPASRESRGGKEGFLH
                     RIGLYPALNWLAGIGTMWVWHIPVLCNASVNDPLVRGIQVGSLLGMGLLFWTPILGSP
                     WGGRLNPWSGIAYLFSACLGCILLGIGITFAPVNVCSTFIDPVDRLGLLSTIRGQWGM
                     TPKLDQQIGGLMMWVPSCLVYLIGILNMLGRGFHQEIPAETVEPRPKAGALPLAAHSK
                     TK"
BASE COUNT          235 a          377 c          435 g          187 t
ORIGIN      
        1 gtgggtgcag gtcagcgcga aggcgatgaa ctcggtctcc gagttgcggc gcagccaggc
       61 gccggtcttg cccgtcgggc cgtcccacgc caagggggcc gcgttgtcga aggaaacggc
      121 caccgtgtgg ttgaccttga aatcgtcgat cttgcccacg cggcgccact tggggacgtc
      181 gctcttgagc acggggccca gcacgtagcc caccagcggg atggacatgg ccagggtgcc
      241 gatgcccccc aggatgaagg tgagcctttc caggaaggcc cggcggctca acagctcggg
      301 cgaggcgggt ttggtctcgt gcggcggttc agtttttgcg gtcattgcgg agctctccaa
      361 tccaaccgat gatggaaacg acaaaggcga tcaagccgcc caaggtgatg tagatcgagg
      421 tcacggcccc ccaggccatg gagaccaccg ccagggcgat gacggtgggc cacaccgtcg
      481 gcggcggcag cttcaggggc ttgggatcgt gccagcccgc ggggcgcggg ggtgcgtcgt
      541 gttgatgcgt ttcgttcgac atggtccggt cctatttcgt cttggaatgg gcggctaacg
      601 gcagggcccc ggccttcggc cggggttcga cggtctcagc ggggatctcc tgatggaacc
      661 cccggcccaa catgttcaag atgccgatca aatacaccaa gcaggaaggc acccacatca
      721 tgagcccccc gatctgctgg tccagcttgg gggtcatgcc ccactggccg cggatggtgg
      781 acaaaaggcc caggcggtcg acggggtcaa tgaaggtgga acagacgttg acgggcgcga
      841 aggtgatccc gatgcccagc aggatgcagc ccaggcaggc gctgaagagg taggcgatgc
      901 ccgaccaggg gttcaaccgg ccgccccaag gggagcccag gatgggcgtc cagaagagca
      961 ggcccatgcc gagcagggac cccacctgga tgccccgcac caggggatcg ttcaccgagg
     1021 cgttgcacaa gaccgggatg tgccagaccc acatggtgcc gatgccggcc agccagttca
     1081 gggccggata aagcccgatg cggtgcagga acccttcctt cccgccgcgg gactcacggc
     1141 tcgcgggcgg gaaccccagc aggaacaaca gggggaccag catgagcagc gccatgtgtt
     1201 ggaacatgtg ggcggtgaaa aggtaccctt ccgc
//
LOCUS       DASTJJ010000526         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1051767, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000526 DASTJJ010000000
VERSION     DASTJJ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051767"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..510)
                     /locus_tag="VFR02_06440"
     CDS             complement(13..510)
                     /locus_tag="VFR02_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET9870114.1"
                     /translation="MSRPKKTRWDKAALGFTLARLASGFILVLASLDKLGGAEKFSKM
                     VENYHALPADLVPLAAVVIPWLEFFTGLCLCLGWRARGAALVYCALMAVYATAIAANL
                     LQGVDMNCGCFSMDSTEKISGWTLARDLGFLALGLAALFARRTYASLDAWRDEGRLRV
                     GDTWF"
     gene            complement(507..1109)
                     /locus_tag="VFR02_06445"
     CDS             complement(507..1109)
                     /locus_tag="VFR02_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9870115.1"
                     /translation="MAPSRPSAKPFAFRATLLEAGGIFLFSAVFGVLFDLFYTEGIEL
                     KVEPPKPVHLQEILKSRPAAYAGWGKPAAAPSPSPSPAPGADGLPRLSLIGAKDRFDR
                     KAAVFLDARSPEEYREGHIPGALDFYADDFEKMAPQVLPQLPNKDREIVAYCHGTSCD
                     LSIHLAEKLAEQGYTHVDIFFGGWPEWKKAGYPIRQGNEP"
     gene            1258..>1516
                     /locus_tag="VFR02_06450"
     CDS             1258..>1516
                     /locus_tag="VFR02_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870116.1"
                     /translation="MAPPIPLNRRTIFVVAFFIILAGLLTLLFSLLEPFLRSFVWATL
                     LVMVFYPVYDRLHRFTRGQDSVAAVLATLIVILCLALPGFFI"
BASE COUNT          264 a          489 c          499 g          264 t
ORIGIN      
        1 agtagcttct ggtcagaacc aggtgtcgcc cacgcgcagg cggccttcgt cgcgccaggc
       61 gtccagggac gcgtaggtcc ggcgggcgaa gagcgccgcc aaccccagcg ccaggaagcc
      121 caggtcccgc gccagggtcc acccactgat cttttccgtc gagtccatcg agaaacaacc
      181 gcagttcatg tccacgccct ggagcaggtt cgccgcgatg gccgtggcgt agaccgccat
      241 gagggcgcaa tagaccaggg cggcgccccg ggcgcgccag cccaggcaaa ggcaaaggcc
      301 ggtgaagaat tccaaccagg ggatcaccac ggccgccaag ggcaccaggt ccgcgggcag
      361 ggcgtggtaa ttttccacca tcttcgagaa tttctccgcg ccgcccagct tgtccaggct
      421 ggccagcacc aggatgaacc ccgaggccaa ccgggccagg gtgaagccca gggcggcctt
      481 gtcccaacgg gtctttttgg ggcggctcat ggttcgttcc cttggcggat ggggtaaccg
      541 gccttcttcc actcgggcca accgccgaag aagatgtcca cgtgggtata gccttgttcg
      601 gcgagcttct cggccaagtg gatggaaagg tcgcaggaag tgccgtggca ataggccacg
      661 atctcccggt ccttgttggg cagttggggc aagacctggg gcgccatctt ctcgaaatca
      721 tcggcataaa aatccagggc ccccgggatg tggccttccc ggtattcctc gggggaacgg
      781 gcgtccaaaa agacggccgc cttgcggtcg aagcggtcct tcgccccgat caggctcagg
      841 cgcggcaggc cgtccgcccc cggagcgggg gaggggctgg gggaaggcgc ggcggcgggc
      901 ttgccccacc cggcgtaggc cgccgggcgg gacttcagga tctcctgcag gtggacgggc
      961 ttcgggggct ccaccttcaa ctcgatgccc tccgtgtaaa aaaggtcgaa aagaacgccg
     1021 aacacggcgg aaaaaaggaa gatccccccg gcctccaaga gggtggcacg gaaggcaaag
     1081 ggcttggcag aggggcggga aggggccatg gcccattatt gaaccgtccg cttttgaatt
     1141 caaggcgggc ccagggcccg gagggccgga aaaagcccgg gggtcccttc cgacttttgc
     1201 agcaatcccg gggcttcggg ggtaaaatgg cggaccttca gcgttaagga gcgacccgtg
     1261 gcgcctccca tccccctcaa ccgccggacc atcttcgtgg tggccttttt catcatcctg
     1321 gcgggcctgt tgaccctgct cttttccctg ttggaaccct tcctgcgttc cttcgtctgg
     1381 gccacgctcc tggtcatggt cttttacccc gtctatgacc ggctgcaccg gttcacccgg
     1441 ggccaagact cggtggcggc cgtgttggcc accctgatcg tgatcctctg cctggccctg
     1501 ccggggttct tcatct
//
LOCUS       DASTJJ010000527         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1785084, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000527 DASTJJ010000000
VERSION     DASTJJ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785084"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFR02_06455"
     CDS             complement(<1..125)
                     /locus_tag="VFR02_06455"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745853.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HET9870117.1"
                     /translation="MILVIDNYDSFTYNLVQYLGEMGADIQVVRNDEATVEELLER"
     gene            complement(122..1690)
                     /gene="trpE"
                     /locus_tag="VFR02_06460"
     CDS             complement(122..1690)
                     /gene="trpE"
                     /locus_tag="VFR02_06460"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9870118.1"
                     /translation="MKPHPSALTPSLNQFKKLARDYDRVPVFLTLQSDLETPLSAYLK
                     LGRGKNAFLLESVEKNEQLARYSIVVFDPTEIFEAKDGLLKRRGQGKPSSAPTKNPLA
                     DIQRLMGAVRQAPLLEAGFLGGLVGYIGYDAVRYFERLPATKPDVLKIPDLYLMATDK
                     LLLFDHLKRTLRVVVNVAVNPRVRPEKAYARAVGEISRLARELAKPLRGAAEIPVPPA
                     PATAPGDLFGFKSNSDPKAFGRMVEDSKEFIKAGDIIQVVGSQRFEKRVATDAVTVYR
                     VLRRLNPSPYMFLLKMEGVELVGSSPEMFIKVQKGLVETRPIAGSRPRGATPAEDLAL
                     EEDLLKDEKELAEHVMLVDLARNDLGRVCEFGSVRVPQFKRVERYSHIMHIVSDVTGR
                     LAAGKTAFDAFQSCFPAGTLSGAPKIRAMEIIEGLEPSRRGIYGGAVAAFGYDGNMDS
                     AITIRSAVLKPAGKGKLAAYVQAGAGLVADSVPQSEYLESCNKARAVLLALHQAEQLG
                     GPARKGGPGKGVRS"
     gene            complement(1804..>2012)
                     /locus_tag="VFR02_06465"
     CDS             complement(1804..>2012)
                     /locus_tag="VFR02_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HET9870119.1"
                     /translation="GEESGNVQKLKALYTDCDKDVVLVKIEQVGDAACHTGNRSCFFN
                     ELSNDQWKDVGVKVFDPEKVYGHK"
BASE COUNT          335 a          656 c          648 g          373 t
ORIGIN      
        1 cgctccagca gctcttccac cgtggcctcg tcgttgcgca ccacctggat gtcggcgccc
       61 atctcgccca ggtactgcac caggttgtag gtgaaggaat cgtagttgtc gatgaccagg
      121 atcatgaccg cacccccttc cccggaccgc cttttcgcgc cggccccccc agctgttcgg
      181 cttggtgcag ggccaacagt accgcccggg ccttgttgca ggactccagg tattcgctct
      241 ggggcacgga gtcggccacc aggcccgccc cggcctgcac gtaggcggcc agcttgccct
      301 tgcccgcggg cttgagcacc gccgagcgga tggtgatggc cgagtccatg ttgccgtcgt
      361 aaccgaaagc cgccaccgcc ccgccgtaga tgccccggcg ggagggctcc aggccctcga
      421 tgatctccat ggcccggatc ttgggggcgc cggacagcgt gcccgccggg aagcaggatt
      481 ggaaggcgtc aaaggccgtc ttgcccgcgg ccagccggcc cgtcacgtcg gacacgatgt
      541 gcatgatgtg ggagtagcgc tccacccgct tgaactgggg cacccgcacg gacccgaact
      601 cgcagacccg gcccaggtcg ttgcgggcca ggtccaccag catgacgtgt tcggccaatt
      661 ccttctcgtc cttcaacagg tcctcttcca gcgccaggtc ctccgccggg gtggcgcccc
      721 gggggcggct gcccgcgatg gggcgggtct ccaccaggcc cttctggacc ttgatgaaca
      781 tctcgggcga ggaccccacc agttccacgc cctccatctt gagcaggaac atgtagggtg
      841 agggattgag gcgccggagc acccggtaga ccgtcaccgc gtcggtcgcc accctcttct
      901 cgaaccgttg ggaccccacc acctggatga tgtccccggc cttgatgaac tccttcgaat
      961 cctccaccat gcggccgaag gccttggggt cgctgttgga tttgaagccg aacaggtcgc
     1021 cgggcgccgt ggcgggggcg gggggcacgg ggatctcggc ggcccccctc aggggcttgg
     1081 ccagctcccg ggccaggcgc gatatttccc cgaccgcccg ggcataggcc ttttcgggac
     1141 ggacccgggg gttgaccgcc acgttcacca cgaccctcag ggtgcgcttc aagtggtcga
     1201 acagcaacag cttgtccgtg gccatcaggt aaaggtcggg gatcttgagg acgtcgggct
     1261 tggtggcggg cagccgctcg aaataacgca ccgcgtcata gccgatatag cccaccagcc
     1321 cgcccaggaa gccggcctcc agcaggggcg cctgccggac ggcgcccatc aggcgctgga
     1381 tgtccgccag ggggttcttg gtgggggccg acgagggttt cccctggccg cggcgcttga
     1441 gcaggccgtc cttggcctcg aagatctccg tggggtcgaa caccacgatg gagtagcggg
     1501 ccaactgttc gttcttttca acgctttcga gcaaaaaggc gttcttgccc cggcccagct
     1561 tcaggtaggc ggacaggggc gtttccaggt cggattgcag ggtgaggaag accgggacgc
     1621 ggtcataatc ccgggcgagt ttcttgaatt gattgaggct gggggtcaaa gcggacggat
     1681 ggggcttcat gtcaaaactc cttcaagttg aatttcaagt ccgggacgaa cggcaacggc
     1741 tgggcgttca gggacgccaa gcaaagccgc gccaagggct caaattcaag ttgaaggagt
     1801 tcctcatttg tggccgtaaa ccttttcggg gtcgaacacc ttgaccccca cgtctttcca
     1861 ttggtcgttg gagagctcgt tgaagaagca ggagcggttg cccgtgtgac aggccgcgtc
     1921 cccgacctgt tctattttta ccagaaccac gtccttgtcg caatcggtat aaagggcctt
     1981 gagcttctgc acattgcccg attcctcgcc ct
//
LOCUS       DASTJJ010000528         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_440215, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000528 DASTJJ010000000
VERSION     DASTJJ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440215"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1149
                     /locus_tag="VFR02_06470"
     rRNA            <1..>1149
                     /locus_tag="VFR02_06470"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          256 a          333 c          401 g          159 t
ORIGIN      
        1 gaccgaacgg tgggaagaac cccgattagg ggaggagact gaacctgaaa ccccgtgcct
       61 acgagcggtg ggagggcgtc tggcttcggc cggcctgacc gcgtgccttt tgcataatga
      121 tccggcgagt tactgtatcc ggccccaggt taaccccttc agggggggag ccgtagcgaa
      181 agcgagtctt catcgggcgc attagtcggg tgcagtagac gcgaaaccag gtgaactacc
      241 catgggcagg ttgaagcggc cctaaccggc cgtggaggac cgaacccacc agtgttgaaa
      301 aactggggga ggacccgtgg ggaggagtca aagtcttatc aaacctggag atagctcgtt
      361 ctctccgaaa tggctttagg gccagcctcg ggacttaccc tgcgggggta gagcgacgga
      421 attcggccgg ggggcttccc gcctacccgc cgaaacccaa ctccgaatac cgggtgcggt
      481 tactccggga gtcagagcgc gggggagaag cttcgcgctc cagagggaaa gaacccagac
      541 cgccgaccaa ggcccccaag acgacgctca gtggcaaagg aagtgggatc gccgtgacag
      601 ccgggatgtt ggcttagaag cagccaccat ttcaaaagtg cgtaacagct taccggtcga
      661 gccatcccgc gccgaaaatg acggggacta agcgtcgcgc cgcagtcgcg gatcgcaaga
      721 tggtaggaga gcgtcgcccg ccgccgagaa gccggacggc aacgaccggt ggagcgcggg
      781 cgagtgcgaa tgccggaacg agtaacgaca agacgggtgg gaagcccgtc cgccgaaagc
      841 acaaggtttc ctggggaagg tcaatccgcc cagggtcagt cgggacctaa ggcgaggccc
      901 aagggcgtag ccgatgggca cgcggttaat attccgcgac ccggcgggga ggttgaggca
      961 ggggtgacgc cggcatgagg gggtacgggg cgctagactg ccccggcccg caagggggac
     1021 ggcacttcga tccggaactc ccctgattgc ccggccacga aaaaccccgt ccggacgacg
     1081 caacggcccg taccgcaatc cgacacaggt gggccaggcg agcatccgaa ggcgttcggg
     1141 agaaccctc
//
LOCUS       DASTJJ010000529         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1052107, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000529 DASTJJ010000000
VERSION     DASTJJ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052107"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /gene="rsfS"
                     /locus_tag="VFR02_06475"
     CDS             <1..259
                     /gene="rsfS"
                     /locus_tag="VFR02_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9870120.1"
                     /translation="GYTDYLVLCAGTSGTHVRALVSAVEGAAGRGGLRVNSSSDDSWW
                     VLDQVDWVVHVFREDARRFYDLEGLWADAAVVKMESQQNSL"
     gene            670..>1366
                     /locus_tag="VFR02_06480"
     CDS             670..>1366
                     /locus_tag="VFR02_06480"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870121.1"
                     /translation="MGGAFVAVADDPSAAYWNPAGLIQLQVPTLELMYGSLFNDKTRN
                     LYGSFQYPLPNDFHLALSVNSVFFGDTDGALQGEYQGSLAVPLPFVPDKRLSVGFNFR
                     YLNADLGGGQGQAQGIGVDLGFLYRQKLEDQMELRAGLVLNDLSTTARFDSGVEENLP
                     TIVTPGLAFRFDKDTLVSLDLPWTVSDYTNQSSDLEFRSGLEHWFFDGKLGLRVGYLS
                     LATLPGEFTVGTSY"
BASE COUNT          250 a          388 c          415 g          313 t
ORIGIN      
        1 cggctacacc gattacctgg tcctgtgcgc cggcacttcg ggcacccatg tgcgggccct
       61 ggtgtcggcg gtggaaggcg cggccggccg gggcggactg cgggtgaatt cctcttccga
      121 tgacagttgg tgggtgctgg atcaagtgga ctgggtggtg catgtctttc gggaggacgc
      181 gcgccgcttt tacgatctgg aagggctttg ggcggacgcg gcggtcgtga agatggaatc
      241 ccagcaaaat tccctttaaa aaccacataa aacaacgagc tcttttgcct ttgacaattg
      301 agaatgctct gattatctta aggttgtcgt ttaacattaa acatttatgt tcacctctgc
      361 aacccctcgg tacctggtcc atcggggaag ccccaaaaaa agcaggaact gcaatatttt
      421 tgtcgttctt tctttatcgt ttaagggtat ttcaaatgta cagattttct gaaagaccgc
      481 taaaatccag atgtttcacg ggggagccgg aaaaggggtc gctggtcaat gcgaatggat
      541 cgttttaatt ttaaattgaa ccttggcttt gtggcctttt tcggtcttct gggtattttt
      601 ttcccacccc gggcccaggc catctttgag cggggcggca acgttggcgc gggcgcccgg
      661 gccatgggga tgggcggcgc tttcgtggcc gtggcggatg accccagcgc ggcctattgg
      721 aatcctgcgg gcctgatcca actgcaggtg cccaccctgg agctgatgta cggctcgctc
      781 ttcaacgaca agacccggaa cctctacggc tcgttccaat atcccctgcc caatgatttc
      841 cacttggcgc tcagcgttaa cagtgtcttc ttcggcgaca ccgacggcgc cctgcagggg
      901 gaataccagg gttccttggc ggtgcccctg cccttcgtcc cggacaaacg cctgagcgtg
      961 gggttcaatt tccgttatct caacgccgac ttgggcgggg gccagggcca ggcgcagggc
     1021 atcggcgtgg acctggggtt cctctaccgc caaaaactgg aagaccagat ggaactgcgg
     1081 gcggggttgg tcttgaacga cctctccacc acggcgcgct tcgacagcgg ggtggaggag
     1141 aatttgccca ccatcgtcac ccccggcctg gccttccggt tcgacaagga caccctggtc
     1201 tcgctggacc tgccttggac ggtctcggac tacaccaacc agtcgtcgga cctggagttc
     1261 cgcagcggct tggagcactg gttcttcgac ggcaagctgg gcctgcgggt gggctacctg
     1321 agcctggcca ccctgccggg cgagttcacc gtggggacca gctacc
//
LOCUS       DASTJJ010000530         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_74117, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000530 DASTJJ010000000
VERSION     DASTJJ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74117"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /gene="trpD"
                     /locus_tag="VFR02_06485"
     CDS             complement(<1..670)
                     /gene="trpD"
                     /locus_tag="VFR02_06485"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9870122.1"
                     /translation="MIRDCILKLSRKEPLTEAEAWDATLEIFEGKAAPAQVGAYLTFL
                     AVKGETPEEILGSMKAMREKMVRLRVEDLDAIDVCGTGGDHKGTFNISTAAALVLAGG
                     GVKVAKHGNRAASSQCGSAEVLEALGVKIDATTLVLEKCLREAGVAFLFAPHFHPAMK
                     HVGPIRKELGFRTLFNILGPLCNPASVKKQVIGVYDKDKAQLMGEVLVKAGSERVVTL
                     HSRDG"
     gene            complement(762..>1133)
                     /locus_tag="VFR02_06490"
     CDS             complement(762..>1133)
                     /locus_tag="VFR02_06490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9870123.1"
                     /translation="DAHEDLYAVLRDEAKGLKVLLHCYSAGPDFVDRFLDLGCHFSIA
                     GPVTFKNGQAHRDAVARIPLDRLVVETDSPYLTPHPHRGKRNEPAYVRLTAETVAQVK
                     GLSLEAIGEITTRNARAFFLI"
BASE COUNT          186 a          361 c          372 g          214 t
ORIGIN      
        1 atccatcacg actgtgcagg gtcacaaccc tttcgctccc cgccttgacc aacacttcgc
       61 ccatgagctg ggccttgtcc ttgtcataga ccccgatgac ctgtttcttc accgaggccg
      121 ggttgcacaa aggccccagg atgttgaaga gggtgcggaa ccccagttcc ttgcggatgg
      181 ggcccacgtg cttcatggcc gggtggaaat gcggggcgaa gaggaaggcc acaccggcct
      241 cccgcaggca tttttccaac accagggtgg tggcgtcgat cttcacgccc agggcttcca
      301 atacctcggc gctgccgcac tggctggagg ccgcgcggtt gccgtgcttg gccaccttca
      361 ccccgccgcc ggccaacacc agggccgccg ccgtggagat gttgaaggtg cccttgtggt
      421 cgccgccggt gccgcaaacg tcgatggcgt ccaggtcctc gacccgcagg cgcaccatct
      481 tctcgcgcat ggccttcatg ctccccagga tctcctcggg ggtctcgccc ttgacggcca
      541 ggaaggtgag ataggcgccc acctgggccg gggcggcctt cccctcgaag atctccaggg
      601 tggcgtccca ggcctcggcc tcggtgaggg gctcttttcg ggataatttg aggatgcagt
      661 cgcggatcat ggcgccttct tcgttcagga aaatgagggg ttattctacc cggattggcg
      721 ggaaatccaa caaaatggcg gtccgcccgg cttcccgggc tttaaattag gaaaaaagcg
      781 cgggcgtttc gagtggtgat ctcgccgatc gcttccaggg agagcccctt cacttgggcc
      841 accgtttcgg ccgtgagccg cacataggcc ggttcattgc gcttgccccg gtggggatgg
      901 ggggtcaggt agggggagtc ggtctccacg actaggcggt ccagggggat ccgggccacc
      961 gcgtcccggt gggcctggcc gttcttgaag gtcaccgggc ccgcgatgga gaaatggcag
     1021 cccaggtcca ggaagcggtc cacgaaatcc ggccccgccg aatagcagtg gagcaggacc
     1081 ttcaggccct tggcctcgtc ccggaggacc gcgtagaggt cctcatgggc gtc
//
LOCUS       DASTJJ010000531         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1174576, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000531 DASTJJ010000000
VERSION     DASTJJ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174576"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFR02_06495"
     CDS             <1..379
                     /locus_tag="VFR02_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870124.1"
                     /translation="KVLAFLIAVGLWAYVGSREVLDRRVVVHLQLTDIPAGMTVDSNV
                     RTAIPVVLTGRKESILALDADDLTAVVSLRDYRPGKPDLPVRPLVRPLPPGVEASAPD
                     VTIHLIPVPKPTPAPHLRRKKGA"
     gene            381..1109
                     /locus_tag="VFR02_06500"
     CDS             381..1109
                     /locus_tag="VFR02_06500"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET9870125.1"
                     /translation="MARLCVNIDHIATLRQARLEEDPDPLAAARLVEKAGAHGITVHL
                     REDRRHIQDRDVTALRRRVRTKLNLEMAATEGMLRFALKVRPDQVTLVPEKRRELTTE
                     GGLRLEGAFGRFERAVDRLQAKGIPVSLFIAPTPRSVRLASRLGATFVELHTGAYSRA
                     FRLGRPTAKPLAAVALAARLASGLGLRVHAGHGLTYQNTAAVARLPEIEDLNTGHNII
                     ARAALVGLERAVREMLAVIRRAKS"
     gene            1283..1600
                     /locus_tag="VFR02_06505"
     CDS             1283..1600
                     /locus_tag="VFR02_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870126.1"
                     /translation="MGKLILFAVVLAAGAVGYLLWSTKPEAPPQPTQPGYVQGLQLSE
                     QHAEAAAAGANLDNVRSALEKYRSLKGSNPASLQDLVPDFLDHVPGGLQYDPATGTVS
                     AAP"
     gene            1601..2119
                     /locus_tag="VFR02_06510"
     CDS             1601..2119
                     /locus_tag="VFR02_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET9870127.1"
                     /translation="MRPPKPLTDEAQALRYALWLLNRRPHSEGELREKFRARKLPPEL
                     GDRVLDRLKAKRFVDDAAFAEGFARSRADRGWGPAKIRAALRLKKIPRETVEASTAGV
                     FPAQDEAAAARELLERQKAKFSPAKDKKRGQGAARALAYLVRRGYSFGAARLAVKGVL
                     GYNSGLPGEDGL"
     gene            2143..>3026
                     /locus_tag="VFR02_06515"
     CDS             2143..>3026
                     /locus_tag="VFR02_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9870128.1"
                     /translation="MTPPTSGSEIRKAYLDFFKSKQHKIVPSSSLVPENDPTLLLSNA
                     GMNQFKPYFLGTIPFPHEVPYAASCQKCFRTGDLERVGYTARHHTFFEMLGNFSFGGY
                     FKKEAIAWAWEFVTEVLKLDRERLWVSVFEKDDEAIELWKKTAGLPAERVVRMGEDSN
                     FWTMGPTGPCGPCSEIYVDLSPEKGAPKDFEAEAESGRFLEIWNLVFTQYDRQEDGAL
                     KPLPKPNIDTGMGLERVASVMQKVPSNFETDLLAPLMAAVAKAGAIAYQADESPAYKS
                     LKVISDHLRATTFLIADGV"
BASE COUNT          504 a         1051 c          974 g          497 t
ORIGIN      
        1 gaaggtcctg gcctttctca tcgcggtggg cctgtgggcc tacgtgggct cacgggaagt
       61 gctggaccgc cgggtggtgg tccacctgca attgaccgac atccccgcgg gcatgacggt
      121 ggattccaac gtgcggaccg ccatcccggt ggtgctgacc ggccgcaagg aaagcatcct
      181 ggccctggac gccgacgacc tgaccgccgt ggtgagcctg cgggactacc gcccgggaaa
      241 acccgacctg cccgtgaggc ccttggtgcg gcccctgccg ccgggcgtgg aggccagcgc
      301 ccccgacgtc accatccatt tgatccccgt gcccaaaccc acgccggcgc cccacctgcg
      361 ccgaaagaaa ggcgcctgac atggcccgtc tctgcgtgaa catcgaccac atcgccaccc
      421 tgcggcaggc ccgtttggag gaggacccgg accccttggc ggccgcccgc ctggtggaaa
      481 aggccggggc ccacggcatc acggtgcacc tgcgggagga ccggcgccac atccaggacc
      541 gggacgtgac ggccctgcgg cgccgggtca ggaccaagct gaacctggag atggcggcca
      601 ccgaggggat gctccgcttc gccttgaagg tgcggcccga ccaagtgacc ctggtgcctg
      661 agaagcgccg ggaactcacc accgaggggg gattgaggct ggagggggcc ttcggccgtt
      721 ttgagcgcgc cgtggaccgg ctgcaggcca agggcatccc ggtgtcgctt ttcatcgcgc
      781 ccaccccccg ttcggtgcgg ctcgcctccc gcctgggggc gaccttcgtg gagctccaca
      841 cgggcgccta tagccgggcc ttccgcctgg gccggcccac cgccaagccc ctggcggccg
      901 tggcgctcgc cgcccggctg gcttccggac tgggcctgcg ggtgcacgcc gggcacggat
      961 tgacctacca aaacaccgcc gccgtggcgc gcctcccgga aattgaggac ctcaatacgg
     1021 gccacaacat catcgcccgg gccgccctgg tgggcctgga acgggcggtc cgggaaatgc
     1081 tggccgtcat ccgtcgggcc aaatcttaag cctgttcacc gcaaagacgc gaaggcgcaa
     1141 aggtgagccg gcccggcttc tcccttcaaa tcgctcacgg cttgttcgcg gtccccgtca
     1201 tttccgcttt tcttagcgtc ttggcgtctt tgcggtaaaa tggccttgtt ccttcttttc
     1261 gacattttct aggaggtgtt ccatgggaaa gctcattctt tttgcggtgg tgttggcggc
     1321 gggggcggtc ggttatcttc tttggagcac caagcccgag gccccgcccc agccgaccca
     1381 gccgggctat gtccagggcc tgcaactgtc cgagcagcac gccgaagccg ccgccgccgg
     1441 cgccaacctg gacaatgtgc gctcggccct ggagaaatac cgcagcttga aggggtccaa
     1501 ccccgcctcg ctccaggacc tggtgccgga tttcctggac cacgtgccgg gcggccttca
     1561 gtacgacccc gccacgggca ccgtttccgc cgcgccctga gtgcggcctc ccaagccgct
     1621 caccgatgag gcccaggccc tccgctacgc cctctggctc ttgaaccgcc gcccccatag
     1681 cgagggggaa ctgcgggaaa aattccgggc ccgcaagctg ccccccgagt tgggggatag
     1741 ggtgctggat cggctcaagg ccaagcgctt cgtggatgac gcggccttcg ccgagggttt
     1801 cgcccggtcg cgggcggacc ggggctgggg cccggccaag atccgcgcgg cgctgcgcct
     1861 caaaaagatc ccccgggaga ccgtggaggc ctcgacggcc ggtgtattcc ccgcgcagga
     1921 cgaggccgca gcggcccggg agcttttgga acgccaaaag gccaagtttt cgcccgcaaa
     1981 agacaaaaaa aggggacaag gggcggcccg ggcgctcgct tacctggtgc gccggggcta
     2041 ttcttttggg gcggcccgtc tcgccgtgaa gggggttttg ggctataatt ccggccttcc
     2101 cggcgaagac gggctgtagc caagccattc cttgaggtcc ccatgacgcc ccccaccagc
     2161 ggcagtgaaa tccgaaaagc ctacctggat ttcttcaagt ccaaacagca caagatcgta
     2221 ccctcctcct ccctggtgcc cgagaacgac cccaccctgc tgttgtccaa cgccggcatg
     2281 aaccagttca agccctattt cctgggaacc atccccttcc cccatgaggt gccctacgcg
     2341 gcctcctgcc agaagtgctt ccgcaccggc gacctggaac gggtgggcta cacggcgcgc
     2401 caccacactt tcttcgagat gctgggcaat ttttccttcg gcggctactt caagaaggaa
     2461 gccatcgcct gggcctggga gttcgtgacc gaggtgctga agctcgaccg ggagcggctc
     2521 tgggtcagcg tgttcgagaa ggacgacgaa gcgatcgaac tgtggaagaa gaccgccgga
     2581 ttgcccgcgg aacgcgtcgt gcgcatgggc gaggattcca acttctggac catgggcccc
     2641 acgggcccct gcggcccctg ctcggagatc tacgtggacc tgtcgcccga aaagggcgcg
     2701 ccgaaggatt tcgaggcgga ggccgagtcg ggccgtttcc tggagatctg gaacctggtc
     2761 ttcacccagt acgaccgcca ggaggacggc gccctgaagc ccctgcccaa gcccaacatc
     2821 gacaccggca tgggcttgga acgggtggcc agcgtcatgc agaaggtgcc ctccaatttc
     2881 gagaccgacc tgttggcgcc cctcatggcg gccgtggcca aggccggcgc catcgcctac
     2941 caggcggacg agtccccggc ctataagtcc ctcaaggtca tcagcgacca cctgcgggcc
     3001 accaccttcc tcatcgccga cggcgt
//
LOCUS       DASTJJ010000532         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1419483, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000532 DASTJJ010000000
VERSION     DASTJJ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419483"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFR02_06520"
     CDS             complement(<1..482)
                     /locus_tag="VFR02_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870129.1"
                     /translation="MGATRSKAQKAGWAKSWGPWAGLLGLLGLWAYFHFQVLFLGHNF
                     VYLDAGNFFYPLWEWGARVWRSGFLPLWDPDAAFGFPAVADPENLTWYPPKIVLYLLF
                     PAGTAFKLLGLGHSLWLLLGFWLFARDRGAAGWPAAFGTVAFGFGFESVALLTGASAL
                     "
     gene            542..973
                     /locus_tag="VFR02_06525"
     CDS             542..973
                     /locus_tag="VFR02_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870130.1"
                     /translation="MNRTSPIHTPPGGPQGRPPSPEIYMKMGQENIFRMLADFYHELG
                     RSQIRGMFPEDLEAASRKSALFFTTLLGGPPLYAQTYGPPRMRARHLPFEIDEGARRI
                     WLDCFDRVLRDAPARYAFPPEHLEDFRRFLADFSAWMVNKA"
     gene            complement(998..1298)
                     /locus_tag="VFR02_06530"
                     /pseudo
     CDS             complement(998..1298)
                     /locus_tag="VFR02_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482368.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
BASE COUNT          276 a          458 c          381 g          183 t
ORIGIN      
        1 aagagggccg aggcgccggt caacagggcg accgattcga acccgaatcc gaaggccact
       61 gtcccgaaag ccgccggcca accggcggct ccccggtccc gggcgaaaag ccaaaaaccc
      121 aatagaagcc aaagggaatg cccgagcccc aagagcttga aggccgtccc cgcggggaaa
      181 agcaggtaaa ggacgatctt cggggggtac caggtgaggt tctcgggatc cgcgacggcc
      241 gggaaaccaa aggccgcgtc cggatcccaa agcggcaaaa aacccgagcg ccaaacgcgc
      301 gcgccccatt cccatagggg ataaaaaaag ttcccggcgt ccagataaac gaaattatgg
      361 cccaggaaga ggacctgaaa atggaaataa gcccagagcc cgagcaggcc cagcagaccg
      421 gcccaggggc cccaagactt ggcccatccc gccttttgag ctttcgaccg cgtcgctccc
      481 accggtttgt cctccaaggg ccgagccccg atttgctaca ctatccgccg gaaaggaaac
      541 catgaaccgg acaagcccca tccatacccc gcccggcggc ccccagggcc gcccccccag
      601 ccccgaaata tacatgaaaa tgggccagga aaatattttc cgcatgctcg ccgatttcta
      661 ccacgaactg ggccgctcgc agatccgggg catgttcccc gaggacttgg aagccgcatc
      721 gcgcaagtcc gcgctctttt tcaccaccct gctgggtggg ccgcccctct acgcccagac
      781 ctacggtccg ccccgcatgc gcgcccggca cctgcccttc gagatcgatg aaggggcccg
      841 gcggatctgg ctggattgtt ttgaccgggt cttgcgggac gccccggccc gttatgcctt
      901 cccgcccgag cacctggagg attttcggcg gttcctggcc gacttttcgg cctggatggt
      961 caacaaggcc tgaaacccgc gggccccggg aaccatttca caagatcaac atcgcgtcgc
     1021 cgtatgaaaa gaagcggtac cttcccggac ggcttcccga taggccgcca ggaccctctc
     1081 ccggcccgcg aaggccgaga ccaacatcaa aagcgtggat tgaggctggt ggaagttcgt
     1141 gaagagggcg tccaccgccc ggaaggcgta tccgggagtg atgaagagcc gcgtttcccc
     1201 ggcccccgcc cgcaagcctt ccccctgggc ggccgattcc aacgcgcggg ccaccgtggt
     1261 ccccagggcc accacccggc cgccccgcgc cctggcgc
//
LOCUS       DASTJJ010000533         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_562762, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000533 DASTJJ010000000
VERSION     DASTJJ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562762"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFR02_06535"
     CDS             <1..511
                     /locus_tag="VFR02_06535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9870131.1"
                     /translation="LTGLYNRRYFQQILDYELNRAKRYQQALSIIFIDLDHFKQINDR
                     FGHSMGDLFLKQISQKLAGMFRTTDLIARYAGDEFVAILPATQQEGAMILAHRIQETL
                     GDYQIMVRGTTLQVSVSIGVDTYENADGIGSITLIDRADKAMYEAKSHGRNCVRSFQE
                     LEKALVKQG"
     gene            complement(831..>1629)
                     /locus_tag="VFR02_06540"
     CDS             complement(831..>1629)
                     /locus_tag="VFR02_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HET9870132.1"
                     /translation="AKGLRVDILRLKTTLQMLQTTSPSYVILASLDMARHQMVKYGEQ
                     ALGEVLAMCEATRQEVNRIPGFSCVTRDQVQKLGPLDLDPTKLTISTKNVPCSGYDMA
                     KILNSEYDIQTEMSDFQNILLFVSMGNTAKELKRLVKALRKIVVDYKDMFVNTKKRRP
                     VVFPHFVPKKEVNPREALTGLTRKIPFKRSVGKVCAEIVCPYPPGIPVLCPGEVITQE
                     IYSYLMNVLESGARINGQSDGRLQTIKVLEDANPSQTAQQKKLFVIN"
BASE COUNT          307 a          511 c          477 g          334 t
ORIGIN      
        1 cctcaccggg ctctataacc ggcgctattt ccagcagatc ctggactacg agctcaaccg
       61 ggccaagcgc taccagcagg ccctgtccat catcttcatc gacctggacc acttcaagca
      121 gatcaacgac cgtttcgggc attccatggg cgacctcttc ctcaaacaga tctcccagaa
      181 actggccggc atgttccgca ccaccgacct catcgcccgc tacgccgggg acgagttcgt
      241 ggccatcctg cccgccaccc agcaggaagg ggccatgatc ctggcccacc gcatccagga
      301 gaccctgggc gactaccaga tcatggtgcg gggcaccacc ctgcaggtct cggtgagcat
      361 cggcgtggac acctacgaga acgccgacgg catcggcagc atcaccctca tcgaccgggc
      421 ggacaaggcc atgtacgagg ccaaatccca cggccgcaac tgcgtgcgca gcttccagga
      481 attggaaaag gccctcgtca agcagggctg atccatcggc aaaaccctga aacctacaat
      541 gatgaacatc tgatcaaggc tgatgcggtc aagcctccag ctttttagac atctgcctgg
      601 atcattgtcg cttttgactt cggcagggca tcggacaact tggccaaggc acaacatcca
      661 cggccatttg caaggatggg cgccgatcca cgccgaggag acgaaaccat ccgatcccac
      721 gcctccatcg gcgtgcatca tcgctgattt ttttgggggg ttgccgtttc cagccttcgg
      781 agcatcaagc ttcatcagcc ttggtcattg ttgctgtgga attcagcctg tcagttgatg
      841 acgaagagtt tcttttgttg ggcggtctgg ctggggttgg cgtcctccag gaccttgatg
      901 gtctggagcc ttccgtcgga ctggccgttg atgcgcgcgc ccgactccaa cacgttcatc
      961 aggtagctgt agatctcctg ggtgatgacc tccccggggc acagcacggg gatgcccggc
     1021 gggtaggggc acacgatctc cgcgcagacc ttccccacgg accgcttgaa ggggatcttg
     1081 cgggtcaggc ccgtcaaggc ctcccggggg ttcacttcct tcttgggcac gaaatggggg
     1141 aacaccacgg gccgccgctt cttggtgttc acgaacatgt ccttgtagtc caccacgatc
     1201 ttccgcaggg ccttcaccag gcgcttcagt tccttggcgg tgttccccat ggacacgaac
     1261 agcaggatgt tctggaaatc cgacatttcg gtctggatgt catattcgga gttcaggatc
     1321 ttggccatgt cgtagcccga gcagggcacg ttcttggtgg agatggtcag cttggtgggg
     1381 tccaggtcca gggggccgag cttttgcacc tggtcgcggg tcacgcagga aaatcccggg
     1441 atccggttga cctcctgccg cgtggcctcg cacatggcca gcacctcgcc cagggcctgc
     1501 tccccgtact tcaccatctg gtggcgcgcc atgtccagcg aggccaggat cacgtaggaa
     1561 gggctggtgg tctggagcat ctgcagggtg gtcttgagcc tgaggatgtc gaccctcagg
     1621 cccttggcg
//
LOCUS       DASTJJ010000534         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_807168, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000534 DASTJJ010000000
VERSION     DASTJJ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807168"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFR02_06545"
     CDS             <1..225
                     /locus_tag="VFR02_06545"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9870133.1"
                     /translation="LGGPDLVTRGFSETLPEDLMAEAKDLMRRVITEEVRDEVTDWET
                     VKETVRKALQRFFSQKSNRRPMILPVIMEI"
     gene            228..626
                     /locus_tag="VFR02_06550"
     CDS             228..626
                     /locus_tag="VFR02_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9870134.1"
                     /translation="MGPAAGRPAVRVGEHDWGRMEWLVEEESAPGAGFSLARMVLNPG
                     MATPTHRHPNCHEALTLLEGSVIERLDGDEHALSPGQTFLAPAGAAHGLRNTGDAPAR
                     MMVVYSAGRRLFETVPEPPAAGPPPRQPAS"
     gene            812..>1083
                     /locus_tag="VFR02_06555"
     CDS             812..>1083
                     /locus_tag="VFR02_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9870135.1"
                     /translation="MARDGRRHFLSVFLTGSAGLLLALSLVPTGENPNLLGRVGSDLS
                     YILYGGFGLMAWLFPGILFLAAFALYRGEPLGRPGLKALGLLLLLAS"
BASE COUNT          161 a          375 c          346 g          201 t
ORIGIN      
        1 ctgggcgggc ccgacctggt gacccggggt ttcagcgaga ccctgccgga ggacctgatg
       61 gccgaggcca aggacctgat gcggcgggtc atcaccgagg aggtccggga cgaggtcacc
      121 gattgggaga ccgtgaagga gaccgtccga aaggcgctcc aacgcttctt ctcgcaaaag
      181 tccaaccgcc ggcccatgat cctgccggtc atcatggaga tctgagcttg gggcctgccg
      241 ccggacgccc cgcggtgcgc gtgggggaac acgattgggg ccgcatggaa tggttggtgg
      301 aggaggagtc cgcccccggc gccgggttca gcctggcccg gatggtcctg aaccccggca
      361 tggccacgcc cacccaccgg catcccaatt gccacgaggc cctcacgctc ctggaagggt
      421 cggtcatcga gcggttggat ggggacgagc acgcgctttc ccccggccag accttcctgg
      481 cccccgcggg ggcggcccat gggctgcgca ataccgggga cgccccggcc cgcatgatgg
      541 tggtctattc ggcgggacgg cgtctttttg aaacggtccc cgaacctccc gccgccggcc
      601 ctccgccgcg gcaaccagct tcctgaaaaa aggcccttcg cgccttcccg gccccgataa
      661 ttctattccg ccgggggccc gcctttgtat aatcgtccct cgaccaactt gaattggact
      721 tattgaatgg gggataagcg ccaatgattt ccatgggccg gtccacggcc ttatggcgtc
      781 ccgggaacaa cgcttcccag gcggcctgct gatggcccgc gacggacgcc gtcacttttt
      841 atccgtattc ctgaccggga gcgccggcct gttgctggcc ctgtccctcg tccccacggg
      901 ggagaacccc aacctgctgg gacgggtcgg gtcggacctt tcctatattt tatacggcgg
      961 gttcggcctg atggcctggc ttttccccgg catcctcttc ctcgccgcct tcgccctcta
     1021 ccggggcgaa cccctgggcc gccccggttt gaaggccctc ggactcttgc tgttgctggc
     1081 ctc
//
LOCUS       DASTJJ010000535         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_319952, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000535 DASTJJ010000000
VERSION     DASTJJ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319952"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VFR02_06560"
     CDS             complement(<1..1207)
                     /locus_tag="VFR02_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870136.1"
                     /translation="MAFKHRAGLFLVLAVWAFTPARPAWPGDLNWIANGDFKHPGGAQ
                     GWVGSLNLQPGRQGEPAVYLENLKPVWNEVSQKVPMPDPAPAAIEISGWMRTQNVVQG
                     EQVWNAARISVVFYDAKGDRLGDWPPTTAQVTGTHDWTLYSHTYPLAPGTAWATVELS
                     LGDCTGRAWFSGLKCLAYDFNDQPLASGQAAHPGLKPPPPAPGPNWILDPGFEDLASR
                     DWNGGQTVPTGRGSGHSRYVENQQAAWTLAQQDVSFRGKTPVSVVYGGWLKTEGVQLG
                     TENYMTARLGIDFRDASGKQVGGWQDSVCKVTGTTAWTHYERAYPVPAGTASAHLDAG
                     LGNCVGKAWVDDLSLELIDAQGAPMTVQLTDRQVSDTSDWYAFQPPTGPSDTTLDLSF
                     LNDKPAGAHG"
     gene            complement(1269..1919)
                     /locus_tag="VFR02_06565"
     CDS             complement(1269..1919)
                     /locus_tag="VFR02_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870137.1"
                     /translation="MDGERLRAVAPFAALSLWWALGPVARVLEPGWFFPAWSAAAVWG
                     TGAAGALGFGWLAWRNQARLAGRRRKAASLLRALAARRRPAADPGENDVILAGRVVSR
                     LVRNADEDLREIGPDFSPASLDRLRRYLPQLLDEVGTEEEGWIRLGVVGTYLGETLCR
                     GGRWRWFFRPDPTLRQFSYTPSLLRRDDGRELDPYGWAAELFGGGKGLEEFWKEAR"
     gene            complement(1943..>2324)
                     /locus_tag="VFR02_06570"
     CDS             complement(1943..>2324)
                     /locus_tag="VFR02_06570"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9870138.1"
                     /translation="GLPGLQLYPFGKERVLGPNAFRFKNIEGDFEEAQAALARSRELL
                     TAGDQSLLVLDEILAAVMVKLVSREEVMALLDLYDRDRRCELVLTGHQVWPELTARAD
                     LVTEMRKEKHYFDQKAPSKEGIEF"
BASE COUNT          372 a          849 c          747 g          356 t
ORIGIN      
        1 acccgtgggc gcccgccggc ttgtcgttga ggaaggaaag gtccaacgtc gtgtcggagg
       61 gccccgtcgg gggctggaag gcgtaccagt ccgaggtgtc gctcacctgc cggtcggtca
      121 attggaccgt catgggcgct ccctgggcgt cgatcagttc cagggaaagg tcgtccaccc
      181 aggccttgcc cacgcagttc cccaacccgg cgtccaggtg cgcggaggcg gtgcccgccg
      241 gcaccggata ggcccgctcg taatgggtcc aagccgtggt gccggtcacc ttgcagaccg
      301 agtcctgcca gccgcccacc tgcttccccg aggcgtcgcg gaaatcgatc cccaaccggg
      361 ccgtcatgta gttttccgtg cccaactgga ccccttcggt cttgagccaa cccccgtaga
      421 ccaccgagac cggcgtcttt ccccggaagg acacgtcctg ctgggcgagg gtccaggccg
      481 cctgctggtt ctccacatac cgggaatgcc cggacccgcg gcccgtgggg acggtctggc
      541 ctccgttcca atcccgggac gccaggtcct cgaaccccgg atccaggatc caattgggcc
      601 cgggcgcggg cggcgggggc ttcagcccgg gatgggccgc ctggcccgag gccaggggtt
      661 ggtcgttgaa atcataggcc aggcatttga gcccgctgaa ccaggcccgt cccgtgcaat
      721 cccccaggga aagttccacc gtggcccagg ccgtgcccgg cgccagggga tacgtgtgcg
      781 aatagagcgt ccaatcatgg gtgccggtga cctgggccgt ggtcgggggc caatcgccca
      841 accggtcccc cttggcgtcg taaaagacca cgctgatgcg cgccgcgttc cagacctgct
      901 cgccctgcac cacgttctgc gtgcgcatcc agcccgagat ctcgatggcc gccggggcgg
      961 gatcgggcat cgggaccttc tggctgacct cgttccaaac gggcttcaag ttctccaggt
     1021 agaccgccgg ttccccttgg cggcccggtt ggaggttgag ggaccccacc caaccctggg
     1081 cgccgccggg atgcttgaaa tcgccgttgg cgatccaatt gaggtcgccc ggccaggcgg
     1141 gccgggccgg ggtgaaagcc cagaccgcca acaccaggaa aagacccgcc ctgtgcttga
     1201 atgccatgtc ccgctccccg acttgtttta ccgccgtccc caacgccgcg cccggaagga
     1261 acgggcggtc accgggcttc cttccagaat tcttccaggc ccttcccccc gccgaagagt
     1321 tccgcggccc aaccgtaagg gtccaattcc ctgccgtcgt cccggcgcag gagggagggc
     1381 gtgtaggaga attggcgcag ggtgggatcg ggcctgaaga accagcgcca acgcccgcct
     1441 cggcagaggg tctcccccag gtaggtgccc accacgccca accggatcca gccctcctcc
     1501 tcggtcccca cctcgtccag caactggggc aggtaacgcc gcagccggtc cagggaagcc
     1561 gggctgaagt ccgggccgat ctcccgcagg tcctcgtcgg cgttgcgcac caaccgggac
     1621 accacccgtc ccgccaggat gacgtcgttc tcgccggggt ccgccgccgg gcgacgtctt
     1681 gccgccaagg cccgcaggag ggacgcggcc ttccgccgcc gcccggcgag cctcgcctgg
     1741 ttgcgccagg ccagccaccc aaagcccagg gcgcccgcgg cccccgtccc ccagaccgcc
     1801 gcggcgctcc aggcgggaaa gaaccaaccc ggttccagga cgcgcgcgac cgggcccagg
     1861 gcccaccaca gggacagcgc cgcgaaaggg gccacggcgc gcagcctctc gccgtccaag
     1921 cgtccccccc gcggcccctg gatcaaaact cgatcccctc tttggagggc gccttctggt
     1981 cgaaataatg tttttccttg cgcatctcgg tgaccaggtc ggcccgggcg gtcaattcgg
     2041 gccagacctg atggccggtc aacaccagtt cgcaacggcg gtcccggtcg taaaggtcga
     2101 gcagcgccat gacctcttcc cggctgacca gcttcaccat gaccgcggcc aggatctcgt
     2161 ccagcaccaa gagggattgg tcccccgccg tcaacagttc ccgggatctg gccagcgcgg
     2221 cctgggcttc ctcgaagtcg ccctcgatgt tcttgaaccg gaaggcgttg ggccccagga
     2281 cccgttcctt gccgaaagga taaagttgaa gcccgggaag gccc
//
LOCUS       DASTJJ010000536         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1542404, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000536 DASTJJ010000000
VERSION     DASTJJ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542404"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFR02_06575"
     CDS             complement(<1..503)
                     /locus_tag="VFR02_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9870139.1"
                     /translation="MDLHQILYSMVEKKASDVHLKAGAMPLFRIDGDLTPEGDTPLTP
                     QELAEVASILMDEKQQRLFFEEHREVDLAYAVEGLARFRTNAMWQRGLPEIIMRVIPQ
                     KIPRIEEIGMPTAVLKQIALEQRGLILVTGITGSGKSTTLAALINEMNENRHAHIVTV
                     EDPIEFV"
     gene            complement(591..>1098)
                     /locus_tag="VFR02_06580"
     CDS             complement(591..>1098)
                     /locus_tag="VFR02_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870140.1"
                     /translation="PPPGLDLSSREIYRYYGDQYFQAAAWREVEGRPAPFFWDHGLAF
                     DPGNGTALEHLARSQARAGNWELAARILRQAVEARPDSAAGWFNLAGIYSRAGLRTQA
                     GDCWGRWSRLGGKGPLAVARSRTSPKSADYYAKLSRRYATMGLGFLALKAGETSRLLS
                     RDPSGGEL"
BASE COUNT          198 a          346 c          352 g          202 t
ORIGIN      
        1 tggacgaact cgatggggtc ctccaccgtc acgatgtggg cgtgccggtt ctcattcatc
       61 tcgttgatga gcgccgccag ggtggtggat ttgcccgacc ccgtgatgcc ggtgaccagg
      121 atgaggccgc gctgttccag cgcgatctgc ttgaggacgg ccgtgggcat gccgatctcc
      181 tcgatgcggg ggatcttctg ggggatgacg cgcatgatga tctcgggcag gccccgctgc
      241 cacatggcgt tggtgcggaa gcgggccagg ccctccacgg cgtaggccag gtccacctcg
      301 cggtgttcct cgaaaaagag gcgctgttgt ttttcatcca tcaggatgga ggccacttcg
      361 gccagttcct ggggggtcag gggggtatcg ccttcggggg tcaggtcgcc gtcgatccgg
      421 aagaggggca tggctccggc cttgaggtgg acgtcggagg ccttcttttc gaccatggaa
      481 tagaggattt ggtgtaggtc catcagtgcc cgcccgggtt tgagatgacc cgggcattat
      541 aacaatttca ggccgggaaa ccgctttatt ttaaagtgaa agtcccccgg tcacaattcg
      601 ccccccgaag gatcgcgcga aagcaggcgg gaggtttccc cggcttttag cgctaaaaat
      661 cccaagccca tggtcgcgta acgtcggctt aacttggcgt aataatcggc ggacttgggg
      721 cttgtccggc tccgggccac cgcgagcgga cccttcccgc ccaaccgcga ccaacggccc
      781 caacaatccc cggcctgggt ccgaagtccg gcccgcgaat agattcccgc caaattaaac
      841 cagccggcgg ccgaatccgg ccgggcctcc accgcctggc gcaggatgcg cgccgccagt
      901 tcccaattcc ccgcccgggc ctgcgaccgc gccaaatgtt ccaaggcagt gccattgccc
      961 ggatcaaaag ccaacccgtg gtcccaaaag aaaggggcgg gccggccttc cacctcccgc
     1021 caggccgcgg cctggaaata ttggtccccg tagtagcggt agatctcccg ggaagaaaga
     1081 tccaaccccg ggggcggc
//
LOCUS       DASTJJ010000537         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_320244, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000537 DASTJJ010000000
VERSION     DASTJJ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320244"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="nuoL"
                     /locus_tag="VFR02_06585"
     CDS             complement(<1..1422)
                     /gene="nuoL"
                     /locus_tag="VFR02_06585"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9870141.1"
                     /translation="MIQHAWLIPALPALCFVLIALFTRRSRALSAGISIGAMAACLAM
                     AAVIFLRLAALDPSARTFTLNWPWLAVPPWQADFGVLIDPLSVNMLLVVTLVSLLVQV
                     YSLGYLKDDERFSTYYSYLSLFTASMLLLVLSTNFLQIFVGWELVGVSSYLLVGFWYF
                     KPEAAEAAKKAFVVNRIGDVGFLMGVATIGMTFGSFDFFQVQGMVAQGLADGTYRAGY
                     IAGIALLLFCGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYMVARTFPL
                     FSADPMALSVVAYVGGFTAFFAASIALAQDDIKRILAYSTLSQLGYMMLALGSGGFTA
                     GMFHLTTHACFKALLFLGAGSVIHAVHSNDIWKMGGLAKPMRVTTATFLVAALAISGI
                     PPLAGFWSKDEIFLAVRDSTLPGHGGLLALAVLTAFLTAFYMFRLFFVVFTGASRGGH
                     PHESPSVMTAPLLVLAFLSITVGW"
     gene            complement(1489..1803)
                     /gene="nuoK"
                     /locus_tag="VFR02_06590"
     CDS             complement(1489..1803)
                     /gene="nuoK"
                     /locus_tag="VFR02_06590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9870142.1"
                     /translation="MSHVPVSHFLWFALALFSVGLFGVLARKNAISVLLGVEMMLNAV
                     NVNYLAFAKYWNWNGLTGPVFVIFIITVGAAEVAVALAIVISVYRQWKSANVDEVREL
                     RG"
     gene            complement(1800..2381)
                     /locus_tag="VFR02_06595"
     CDS             complement(1800..2381)
                     /locus_tag="VFR02_06595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9870143.1"
                     /translation="MADAIFYGLAALSLVSALMAVSLRNLFHCALFWVLAMFGVAGVF
                     LYLNAEFLAAVQVIIYVGAVTVFIFFGIMFTRNVMDDRIRVMNRQFVLAVLAAAGFGW
                     LGMKAVAVSPFHGSEHPLVLMQRPSADGRGGTATVATTVGAVDASAQVDVEALARELL
                     SPGDGYDYPFELVSILLLSALVGAIVVARKEPS"
     gene            complement(2384..>2733)
                     /locus_tag="VFR02_06600"
     CDS             complement(2384..>2733)
                     /locus_tag="VFR02_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9870144.1"
                     /translation="DEKDMFNCVACLMCMKACPNSCIKLEGVRMPDGKRRMTTFKLDF
                     STCIFCGYCVEACGFDALTFVSEEYENSVYKRGSLLQDEQAMKWVRPAHLKPAPEEKA
                     PPAAAPAAPQAGA"
BASE COUNT          605 a          882 c          885 g          361 t
ORIGIN      
        1 ccaacccacc gtgatggaca ggaacgccag gaccaacagg ggcgcggtca tgaccgaagg
       61 cgactcgtgg ggatggcctc cccgggaagc gcccgtgaag accacgaaga agagccggaa
      121 catgtagaag gccgtgagga aggccgtcag gaccgccagg gccaacagtc ccccatggcc
      181 cggcagggtg ctgtcgcgca ccgccaggaa gatctcatcc tttgaccaga aacccgccaa
      241 gggtgggatc cccgaaatgg ccagggcggc caccaggaag gtggccgtgg tcacccgcat
      301 gggcttggcc aagccgccca tcttccagat gtcgttggaa tgcacggcgt ggatcacgct
      361 gcccgccccc aggaacagca aggccttgaa acaggcgtgg gtggtgaggt ggaacatgcc
      421 cgccgtgaag ccccccgaac ccaaggccag catcatgtaa cccaactggg aaagggtcga
      481 ataggccagg atgcgcttga tgtcgtcctg tgccaacgcg atggaggccg cgaagaaggc
      541 cgtgaagccc cccacgtagg ccaccacgct cagggccatg ggatcggcgg agaaaagcgg
      601 gaaggtccgg gccaccatgt agacgcccgc cgccaccatg gtggcggcgt ggatgagggc
      661 ggacacgggc gtggggcctt ccatggcgtc gggcagccac acatggagcg ggaactgggc
      721 gctcttcccc accgccccgc agaacaagag cagggcgatg ccggcgatgt agcccgcccg
      781 ataagtgccg tcggccaggc cctgggccac catcccctgg acctggaaga aatcgaagct
      841 tccgaaggtc atgccgatgg tggccacgcc catcaggaac cccacatcgc cgatgcggtt
      901 gaccacgaag gccttcttgg cggcttcggc ggcctcgggc ttgaagtacc agaagccgac
      961 caacaggtag gagctcaccc ccaccaattc ccaacccacg aagatctgga ggaagttggt
     1021 ggacaacacc aacagcagca tggaggccgt gaagagggac aagtaggaat aataagtgga
     1081 gaaccgctcg tcgtccttca ggtagcccag cgaatagacc tgcaccagca acgacaccag
     1141 ggtgaccacc agcagcatgt tgacgctcaa ggggtcgatg aggacgccga aatcggcctg
     1201 ccagggcggc acggccagcc agggccagtt cagggtgaag gtgcgggccg aagggtccaa
     1261 ggccgccagg cgcaggaaga tcaccgccgc catggccagg caggcggcca tggccccgat
     1321 ggagatcccc gcggacaggg cccgggaacg ccgggtaaag agggcgatca ggacgaagca
     1381 aagggccggc agggccggga tcaaccacgc gtgctggatc atgaaggacg ttcccccttg
     1441 cgaaatgaag cgaaaccgtt caaccaaaag gcaaaaagac gccccgggtc agccccggag
     1501 ctcgcggacc tcgtccacat tggcgctctt ccactggcgg tagaccgaga tcacgatggc
     1561 cagggccacc gccacctcgg ccgcccccac cgtgatgatg aagatcacga agaccgggcc
     1621 ggtcagcccg ttccagttcc agtacttggc gaaggccagg tagttcacgt tcacggcgtt
     1681 caacatcatc tccacgccca gcagcaccga gatggcgttc ttgcgggcca agaccccgaa
     1741 caggcccacg ctgaagaggg ccagggcgaa ccaaaggaag tgggaaacgg gcacgtggct
     1801 cacgagggct ccttccgggc caccacaatg gcgcccacca aggcggatag cagcaggatg
     1861 gagaccaatt cgaagggata atcgtagccg tcgcccgggc tgagcagttc ccgggccaag
     1921 gcttccacgt ccacctgggc gctggcgtcc accgccccca ccgtggtggc caccgtggcc
     1981 gtcccgcccc ggccgtccgc cgacggccgc tgcatcaaga ccaagggatg ttccgaaccg
     2041 tggaagggcg aaacggcgac ggccttcatc cccaaccagc cgaaaccggc cgccgccagg
     2101 accgccagga cgaattggcg gttcatgacc cggatgcggt catccatgac gttgcgcgtg
     2161 aacatgatgc cgaagaagat gaagaccgtg accgcgccca cgtagatgat cacctgcacc
     2221 gccgccagga actcggcgtt caggtagagg aagacgcctg ccacgccgaa catggccagc
     2281 acccagaaga gggcgcaatg gaagaggttg cgcagggaga cggccatcag ggccgaaacc
     2341 agggacaggg ccgccaggcc gtagaagatc gcgtcggcca tggtcaagcc cccgcctggg
     2401 gcgccgcggg cgcggcggcc gggggcgctt tttcctcggg cgccggcttc aagtgcgccg
     2461 gtctcaccca tttcatggcc tgttcgtcct gcagcaggct cccccgctta tagaccgaat
     2521 tctcgtattc ctcggacacg aaggtcagcg cgtcgaaacc gcaggcctcc acgcaataac
     2581 cgcaaaagat gcaggtcgag aaatccaact tgaaggtcgt catgcggcgc ttgccgtccg
     2641 gcatgcgcac gccctccagc ttgatgcagc tgttggggca ggccttcatg cacatcaggc
     2701 aggccacgca gttgaacatg tccttttcgt cgt
//
LOCUS       DASTJJ010000538         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1175425, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000538 DASTJJ010000000
VERSION     DASTJJ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175425"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFR02_06605"
     CDS             complement(<1..354)
                     /locus_tag="VFR02_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870145.1"
                     /translation="MKFWNRLGMGTLAVLLALSLGLGQSLAAGKKETKKKKEEKKKEE
                     THHVARMEYRGAMMPIAGTNLSGKPGYFFSDTAEPLAKGQWMGAGHLTFDTWGNQFEI
                     PVGVSYGITDKLLVNV"
     gene            complement(430..>1982)
                     /locus_tag="VFR02_06610"
     CDS             complement(430..>1982)
                     /locus_tag="VFR02_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870146.1"
                     /translation="RRGEERALVEALFQFQPPHPLLDRLREAGFPAPDGEVLLKREFT
                     RSSGKTRSFLNGETATAALVAELGGRLIDIHGQHEHQAIFNVAAHRRLVDELGHLAPF
                     LEKASGAAQALQALLAERDALGGDAAQVERRMDLLAFQVGEIEGSGVLEMDEEALQAK
                     FQRARHAEKIAKALTEAQTSLDPDEGGADGALRAALARLREAAKLDPALEDLLAQGDA
                     LRESLGQFSFDLGKRLGGYSFREDEFQELSDRIDLLHKMKKKYASPSDGIRGFHEAAC
                     AELGSLKGREARLGQLEGEIPQAARAYAQAAAALGAKRLEAGRTLARQVEKALAELGL
                     ANARLEVAVTPQEWAESPAEWEGRRLQVSPQGWDRVEFLFSANPGEPTRPLAKVASGG
                     EASRVMLALKTVLADSDDIPTLIFDEIDTGVGARTAPAVSELLGRLARSKQVLCISHL
                     APIAGLGDWHFQVSKAVEGGATSVRVIRLTEAQRIEELARMLGGEPLSETSLSHAQEL
                     YARMRTVG"
BASE COUNT          271 a          698 c          662 g          351 t
ORIGIN      
        1 cacgttcacc aacagcttgt cggtgatgcc gtagctgacg cccacgggga tctcgaactg
       61 gttgccccag gtgtcgaagg tcaggtgccc ggcgcccatc cattggccct tggcgagggg
      121 ttcggccgtg tcggagaaga aataaccggg cttgccgctc aggttggtgc ccgcgatggg
      181 catcatggcg ccgcggtatt ccattcgcgc gacgtggtgc gtctcctctt ttttcttctc
      241 ttccttcttc tttttggttt ccttcttgcc cgcggccagc gattggccca ggcccaagct
      301 caaggccagc agaacggcca gcgttcccat gcccaaacgg ttccaaaact tcatgtcgtc
      361 ctccagggtt cggctgcgat ttttaaacaa tgggcgcctt tcggcgctcc ccctgccggg
      421 gccttcggct cacccgacgg tgcgcattct agcatagagc tcttgggcgt gggaaagcga
      481 agtttcggac aggggctcgc cgcccaacat gcgggccagc tcctcgatgc gttgcgcctc
      541 cgttaaacgg ataaccctga ccgaggtcgc gcccccttcc acggccttgc tcacctggaa
      601 atgccagtcc cccaaccccg cgatgggcgc cagatgggag atgcagagca cctgtttgct
      661 gcgggcgagc cgcccgagca gttcggagac cgccggggcc gtgcgcgcgc ccacgcccgt
      721 gtcgatctcg tcgaagatca gcgtggggat gtcgtcgctg tccgccagga cggtcttgag
      781 cgccagcatg acccgggacg cctcgccgcc cgaggccacc ttggccagcg gccgggtcgg
      841 ctccccggga ttggccgaga agaggaactc cacccggtcc catccctggg gggagacctg
      901 caggcgtcgg ccctcccatt cggccgggct ttccgcccat tcctggggcg tcacggccac
      961 ctccaggcgc gcgttggcca ggcccagttc ggccagggcc ttttccacct gccgggccag
     1021 cgtccggccc gcttccagcc gcttcgcgcc caaggccgcg gcggcctggg cgtaggcccg
     1081 ggcggcctgg gggatctccc cttccaactg gcccaggcgc gcttcccggc ccttgagcga
     1141 tcccagctcg gcgcaagcgg cctcgtgaaa tccccggatc ccgtcggacg gggaggcgta
     1201 ttttttcttc atcttgtgca gcaggtcgat gcggtccgaa agttcctgga actcgtcctc
     1261 gcggaaggaa taaccgccca ggcgcttgcc caggtcgaag gagaactggc cgagggattc
     1321 ccgcagggcg tcgccctggg ccaagaggtc ctccagggcc gggtccagct tggccgcctc
     1381 gcgcaggcgc gccagggccg cccggagcgc gccgtcggcg ccgccctcgt ccgggtccag
     1441 ggacgtctgg gcctcggtga gggccttggc gatcttttcg gcgtgccgcg cgcgttggaa
     1501 cttggcctgc agggcctctt cgtccatttc cagcacgccc gaaccctcga tctcgcccac
     1561 ctggaaggcc agcaggtcca tgcgccgctc cacctgggcc gcgtcgccgc ccagggcgtc
     1621 ccgttcggcg agcagcgcct gcagggcctg ggcggccccg gaggcctttt ccaggaaggg
     1681 ggccaggtgt cccagctcgt ccaccagccg gcggtgggcc gccacgttga agatggcctg
     1741 gtgctcgtgc tggccgtgga tgtcgatcag ccggccgccc aattcggcga ccagggcggc
     1801 ggtggccgtc tccccgttga ggaagctgcg ggtcttgccc gaggagcggg tgaattcgcg
     1861 tttcaagagc acttccccgt cgggggcggg gaacccggcc tcccgcagcc ggtccaacag
     1921 gggatggggc ggctggaact ggaagagggc ctccaccagg gcccgttcct cgccccggcg
     1981 ca
//
LOCUS       DASTJJ010000539         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1665557, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000539 DASTJJ010000000
VERSION     DASTJJ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665557"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFR02_06615"
     CDS             <1..405
                     /locus_tag="VFR02_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870147.1"
                     /translation="WVWPRGGDVDDQLAWASQEILNALIPHGFTVSKEEGNYDGEDTS
                     VPQWRQWTFTIEGSEVVTQVYIKPVRHRVWVRVGPSEPPAGRSLAEVKAIQKQDLRML
                     KLLRAKFGKRFTPHHRHDWAAPYNHRTGTADE"
     gene            411..563
                     /locus_tag="VFR02_06620"
     CDS             411..563
                     /locus_tag="VFR02_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870148.1"
                     /translation="MSRPVLALVLFAVGILGLCFALGRTYYLVWKLRKGREPVRKRPD
                     PPGDAP"
     gene            591..1064
                     /locus_tag="VFR02_06625"
     CDS             591..1064
                     /locus_tag="VFR02_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9870149.1"
                     /translation="MAAKKEKPADFLREVSLFDGVEDKILGHIFHLGKVKHYSKGESI
                     IREGEAGGNLHIIIEGSAEVSLKKDKQDRSRYLADLERGSIFGEMSVFDAAPYSATVK
                     AREECSVHVIKGEDFKKFLKQNPQEGYVIFTALVKQLSSRLRRTNLAFSLLGFDS"
     gene            1115..>1911
                     /locus_tag="VFR02_06630"
     CDS             1115..>1911
                     /locus_tag="VFR02_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9870150.1"
                     /translation="MKSPLSAKTVAVVSLGCPKNTVDSEVMLGKLAQDRYTLVKDPSQ
                     AEVVIVNTCGFIEASKKESIDTILEVAKYRKSGNLKSLVVGGCLAHRYGDELKRELPE
                     IDILFGLNEVEAVADRIRGEALPLHPAMDKRAPLLPQSMDEATYVYDASSPRLLSTPS
                     HFAYVKISEGCDLPCAFCIIPKIRGHYRSRSLEDIEAEARGLAAQGVRELCLVAQDST
                     WYGSDRYGRPRVAELLEKLARVDPLEWVRLHYLYPSRVSDEMLKVMA"
BASE COUNT          358 a          620 c          610 g          323 t
ORIGIN      
        1 tgggtctggc cccggggtgg ggatgtggac gaccagttgg cctgggcttc ccaggagatc
       61 ctcaacgccc tcatccccca cggtttcacc gtgtccaagg aagaggggaa ctatgacggg
      121 gaggacacca gcgtgcccca atggcgccag tggaccttca cgatcgaggg gtccgaagtg
      181 gtgacccagg tctatatcaa gcccgtccgg caccgggtct gggtgcgggt cgggccctcc
      241 gaaccgcccg cgggccgcag cctggcggaa gtgaaggcca tccaaaaaca ggacctgcgc
      301 atgctcaaac tgctgcgggc caagttcggc aagcggttca cgccccacca ccgccatgat
      361 tgggcggccc cctacaacca ccggacgggc acggcggacg agtaggcccc atgtcccgcc
      421 cggtcctggc cctggtcctc ttcgcggtgg gcatcctggg cttgtgtttc gccctgggcc
      481 ggacctatta tctggtctgg aaactgcgca agggccggga acccgtccgc aaacggcccg
      541 atccacccgg cgacgcgccc tgacccaatc ggacccaagc ggaggaaccc atggcggcga
      601 aaaaagagaa gccggcggat tttttgaggg aggtcagcct ctttgacggg gtggaggaca
      661 agatcctcgg ccacatcttc cacttgggca aggtcaagca ttacagcaag ggcgagagca
      721 tcatccggga gggggaggcc ggcggcaacc tccacatcat catcgagggc tcggccgaag
      781 tgagcctcaa gaaggacaag caggaccgtt cccgctacct ggcggacctg gaacggggtt
      841 ccatcttcgg cgagatgagc gtcttcgacg ccgcccccta ttcggccacc gtgaaggccc
      901 gcgaggaatg ctcggtgcac gtcatcaagg gcgaggattt caagaaattc ctcaagcaga
      961 acccccaaga gggctatgtc atcttcacgg ccctggtcaa gcagctttcc agccgcctgc
     1021 gccgcaccaa cctggccttt tcgttgctgg gcttcgattc ctgaaggcgg ggcgggcttc
     1081 ccatccgccc ggtctgtgct aaagtgtccc ccttatgaaa agcccccttt ccgccaaaac
     1141 cgtcgccgtg gtgagcctgg gatgccccaa gaacaccgtg gattccgagg tcatgttggg
     1201 caagctcgcc caggaccggt acaccctggt gaaggaccct tcccaggccg aagtggtcat
     1261 cgtgaacacc tgcggcttca tcgaggcctc gaagaaggag tccatcgaca ccatcctgga
     1321 ggtggccaaa taccgcaaga gcgggaacct gaagtcgctg gtggtggggg gctgcctggc
     1381 gcaccgctac ggcgatgagc tgaagcggga actgcccgag atcgacatcc tcttcggcct
     1441 gaacgaggtc gaggcggtgg cggaccgcat ccgcggggaa gccctgcccc tgcatcccgc
     1501 catggacaaa cgggcgccgc tcttgcccca gtccatggac gaggccacct acgtgtatga
     1561 cgcctcctcg ccccggctgc tgtccacgcc ctcccatttc gcctacgtga agatatcgga
     1621 aggctgcgac cttccctgcg ccttctgcat catccccaag atccgcggcc attaccgcag
     1681 ccgttccttg gaggacatcg aggccgaggc ccgggggctg gccgcccagg gtgtccggga
     1741 actctgcctg gtggcccagg acagcacctg gtacgggtcg gaccgctacg gccgtccccg
     1801 ggtggccgaa ttgttggaga agttggcccg ggtggaccct ttggaatggg tgcgcctgca
     1861 ttacctctat cccagccggg tgtcggacga gatgctgaag gtgatggcgg a
//
LOCUS       DASTJJ010000540         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1543253, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000540 DASTJJ010000000
VERSION     DASTJJ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543253"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="VFR02_06635"
     CDS             <1..>1156
                     /locus_tag="VFR02_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870151.1"
                     /translation="VLSTLHTMDTIQTMERILDFFPTEQQNQIRVQLSNTLKAVISQR
                     LVSKADGIGVVPACEIMIVNQTIKSLISDNKIGQIRGFIAEGQSQYGMQTFDQSLISL
                     VRAGSISRESAMEQATSPSELDLGLKGISSSRASAQSLISQMESSQSKERIGGWLKRA
                     QDLYDRKRFDECRAELKRILQEMPDHKEANTLMARIREMEAAGEKKKDATANIKAALQ
                     LYRERKHQSAIIEFQKALEIDPENKQAQAYIKAIGEEMANREKARLVIQEAQIANQRG
                     EYDKALSEIEQALVLDPENETAKVLHREIKIAGQKAQAKARAADLNQSAIELYKGGDL
                     LGALVAWNQAFEINSEMEDVARYLQQGLAKLLSFGVEGVESHPDKAAILA"
BASE COUNT          264 a          365 c          358 g          169 t
ORIGIN      
        1 tggtcctttc caccctgcat accatggaca ccatccagac catggaacgc atcctggact
       61 ttttccccac cgaacagcag aaccagatcc gcgtccagct gtccaacacc ctgaaggcgg
      121 tcatttccca gcgtttggtc agcaaggccg acggcatcgg ggtggtgccg gcctgcgaga
      181 tcatgatcgt gaaccagacc atcaagtcgc tgatctcgga caacaagatc gggcagatcc
      241 gcggcttcat cgccgagggg cagagccagt acggcatgca gacattcgac cagtccctca
      301 tttccctggt gcgggccggg tcgatcagcc gggaaagcgc catggaacag gccacgtccc
      361 ccagcgagct ggacctgggc ttgaagggca tctcctcctc cagggcctcg gcccagtcgc
      421 tcatttccca gatggagtcc tcccagtcca aggaacgcat cggggggtgg ctcaaacggg
      481 cccaggacct gtacgaccgc aaacggttcg acgaatgccg ggccgagttg aagcgcatcc
      541 tgcaggaaat gcccgaccat aaggaagcca acaccctgat ggcgcgcatc cgcgagatgg
      601 aggccgccgg ggaaaagaag aaggacgcca cggccaacat caaggccgcc ctccagcttt
      661 accgcgaacg gaagcaccag tcggccatca tcgagttcca gaaggccctg gagatcgacc
      721 ccgagaacaa acaggcccag gcctacatca aggccatcgg tgaggaaatg gccaaccggg
      781 aaaaggcccg cctggtgatc caggaggccc agatcgccaa ccagcggggc gagtacgaca
      841 aggccctgtc cgagatcgag caggcgctgg tgttggaccc ggagaatgag acggccaagg
      901 tgctgcaccg cgagatcaag atcgccggcc agaaggccca ggccaaggcg cgcgcggccg
      961 acctgaacca atcggccatc gagctctata agggggggga cctgttggga gccttggtgg
     1021 cctggaacca ggccttcgag atcaactccg aaatggagga cgtggcccgc tatctccaac
     1081 agggattggc caagttgctg tccttcgggg tggagggcgt ggaatcccac ccggacaagg
     1141 cggcgatcct ggccat
//
LOCUS       DASTJJ010000541         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_442772, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000541 DASTJJ010000000
VERSION     DASTJJ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442772"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFR02_06640"
     CDS             complement(<1..450)
                     /locus_tag="VFR02_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9870152.1"
                     /translation="MSPLELQNITKDFPGVRALDGVSLTLEKGEVHALCGENGAGKST
                     LIKILCGVHPTGSYGGEILLDGKPVAFQGLQDSEKAGLALIAQELALVPGFSVMENMA
                     LGREPKKLGLIQWAEEEKVCREALAQVGLAANPHIPVRDLGVGQQQMV"
     gene            complement(538..1176)
                     /locus_tag="VFR02_06645"
     CDS             complement(538..1176)
                     /locus_tag="VFR02_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9870153.1"
                     /translation="MKKLLFAALFISVASLGWSQTAGSDAKKWELQLQGGLGLPVSPY
                     FAGGYPGESAAKFNTGFNFGGAFGYKISPQLSVLADLEMHDFRSNVITSSPNYYYFWD
                     TTQLSLLLKYRFLTGPITPFIFGGPGVVLNEAGYNYEYPSPGAYSYELGAFDALLQAG
                     VGVEYALNEDFSVFAQASYIIDFNDAYLVDYVEGADMPTRWVPVQAGVILNL"
BASE COUNT          297 a          405 c          368 g          266 t
ORIGIN      
        1 caccatctgc tgctggccta cacccaaatc cctgaccggg atatggggat tggccgctag
       61 tcccacctgg gccagagctt cccggcaaac cttttcttcc tcggcccatt ggatgaggcc
      121 cagtttcttg ggctcccggc ccagggccat attctccatc accgaaaacc cagggaccag
      181 ggccagttcc tgggcgatga gggccagccc cgctttttcc gaatcctgca ggccttggaa
      241 ggccacaggc ttcccgtcca ataggatctc cccgccatag gaacccgtcg ggtgcacccc
      301 gcagaggatc ttgatgagcg tgcttttgcc cgcgccgttc tccccacaga gggcgtggac
      361 ctcacccttt tccaaggtca aggagacgcc gtccagcgcc cgcacgcccg ggaagtcctt
      421 ggtgatgttc tgaagttcga gcgggctcat ggggccatta tgcccgattc ctgaaaaccc
      481 ctaaaaacaa aaaagcccgc cccgcgctgc gcggaacggg cctttctgcg aagaagttta
      541 gaggttgagg atgacacccg cctggaccgg cacccaacga gtgggcatgt cggcgccctc
      601 gacgtaatcg accaagtaag cgtcgttgaa gtcgatgata taggaagcct gggcgaagac
      661 gctgaaatct tcgttcaagg cgtactccac acccaccccg gcttgcaaca gggcgtcaaa
      721 cgccccgagt tcgtaggagt aagcgcccgg agaaggatat tcgtaattat atcccgcctc
      781 gttcaagaca acgccaggtc cgccgaaaat gaaaggcgta atcgggccgg tcaagaaccg
      841 gtatttcagg agcagcgaca gttgggtcgt gtcccaaaaa taatagtagt tggggctgct
      901 ggtgatgaca ttcgacctga agtcatgcat ttccaggtcg gccaaaacgg aaagttgcgg
      961 gctgatctta tacccgaacg cccccccgaa gttgaaaccc gtattgaact tagcggctga
     1021 ctcgccggga taaccgccgg cgaaataggg gctgaccggc aggcccaagc cgccttgcaa
     1081 ctgcagctcc cacttcttgg cgtccgaccc ggccgtctgg gaccagccca aagaagcaac
     1141 cgagatgaaa agcgcggcaa acagtaattt tttcattttt ttctcctttc cgttaaatgt
     1201 ggaggtcaac gccgatttgc accggcacgt agttcaagtc gctgtcgatt tcccagctgt
     1261 ccgccgcggc cttgtcccac caaatgtagg aaagcttggt ttgggcgaaa acgctcaatc
     1321 cgtcattgac ggggag
//
LOCUS       DASTJJ010000542         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1787172, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000542 DASTJJ010000000
VERSION     DASTJJ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787172"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..631
                     /locus_tag="VFR02_06650"
     CDS             455..631
                     /locus_tag="VFR02_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870154.1"
                     /translation="MRSRLRRPALPTPSGQAAVEYLLLLGLVFTVFLGASSLFSKQVQ
                     NYLDLLFGTLCPPF"
     gene            666..899
                     /locus_tag="VFR02_06655"
     CDS             666..899
                     /locus_tag="VFR02_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010560147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HET9870155.1"
                     /translation="MKQPDQRGQGMTEYILIVALIAVLAIGAIKFFGKSTASNFNAAA
                     NATNAAVGSGLNGGGSDAGSAVNNAVGSVTGGH"
     gene            938..1453
                     /locus_tag="VFR02_06660"
     CDS             938..1453
                     /locus_tag="VFR02_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET9870156.1"
                     /translation="MVEFTLAVPLLFLLFLGTIQLAYLSYFYFAVQRAAFAICRDAAA
                     SPDPEKFDPTFDLAASLLPLLKLHPQILLSILGAECRIQASGRQVDVSLRCPMPLWVP
                     PFARLFGQGQAFPIPAASPDLAAVLAPLHLPAGILAAAPAAPMAVHWFNFSASALNED
                     GVTGPSPAEAP"
     gene            1450..>1630
                     /locus_tag="VFR02_06665"
     CDS             1450..>1630
                     /locus_tag="VFR02_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870157.1"
                     /translation="MRRLHPALAGPRGQVLLATLLFCFIFTVLFAGLFRSGLLYGLKE
                     RSVRACDLTALSSGAV"
BASE COUNT          250 a          605 c          435 g          340 t
ORIGIN      
        1 ccagctcact cacccggtcg ggcgccggca tttccgcgcc gcactccgaa cagaactttg
       61 ccttgggatc gtttttagcg ccgcagcggg gacatttcat gaatcggggg tttctcctcg
      121 ggaagttcgg gcaactatac cagccccgtc ccccatttca agggttccat gggccgtttt
      181 aacgttggat tcttcactat ataacggcct ccaggtcgaa aaccttagcc gccgccaagg
      241 gaaaagggtc ccttccccgg ttgggacggc ccgaaacgct gaaaagttgc ccaccccctc
      301 cccttttcgc cccccgcccc ccgccctccc ggggcgccgt ccgcccggcc ccgacaagcc
      361 cgactaggac atcctttatg caaaatataa ttatgtccaa tttttgcgac catccatctt
      421 gaagttcaac gcgccatccg ttagaaactt gcacatgcga tccaggctcc ggcgccccgc
      481 ccttcccaca ccctccggac aagccgccgt ggaatacctg ctgctgctcg gtttggtctt
      541 cacggtcttc ctcggggcct cgtcgctctt ttcaaagcag gtccaaaatt atttggacct
      601 gttatttgga acgctttgtc cacctttttg accgaccttt ccaggaggga cccgtgccga
      661 tccatatgaa acaaccggac caacgaggcc aagggatgac cgaatacatc ttgatcgtgg
      721 ccttgatcgc cgtcctcgcc attggggcca tcaagttctt cggcaagagc accgcctcga
      781 acttcaacgc ggccgccaac gccaccaacg cggcggtcgg ctcggggctc aatgggggcg
      841 gttcggacgc cggctccgcc gtcaacaacg ccgtcgggtc ggtcaccggc ggccattgat
      901 cccacgcgca agatcccggg gcggccgcgg ccaaggcttg gtcgaattca ccttggccgt
      961 ccccctgctc ttcctgcttt tcctggggac catccaattg gcttacctgt cctattttta
     1021 cttcgccgtc caacgggccg ccttcgccat ctgccgtgac gccgccgcca gccccgatcc
     1081 cgagaaattc gaccccacct tcgacctggc ggcgtccttg ctgccgttgc tcaagctgca
     1141 tccccagatc ctcctcagca tcctgggcgc cgaatgccgc atccaggcgt cgggccgtca
     1201 ggtggacgtc tccctgcgct gtcccatgcc gctttgggtg ccccccttcg cccgcctctt
     1261 cggacaaggg caagcttttc ccatccccgc ggcctccccg gacctggccg cggtcctggc
     1321 cccgctccat ctcccggccg ggatcctcgc cgccgcgccg gccgccccca tggccgtcca
     1381 ttggttcaac ttcagcgcct cggcgttgaa cgaggacggc gtgaccgggc caagcccggc
     1441 ggaggcccca tgaggcgcct tcacccggcc ctggcggggc ccaggggcca ggtcctcctg
     1501 gccaccctgt tgttctgctt cattttcacg gtcctgttcg cgggactctt ccggtcgggc
     1561 ctgctgtacg gcctgaagga acggtccgtc cgcgcctgcg acctgaccgc cctttcgtcc
     1621 ggggccgttt
//
LOCUS       DASTJJ010000543         1994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_76578, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000543 DASTJJ010000000
VERSION     DASTJJ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76578"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..765)
                     /locus_tag="VFR02_06670"
     CDS             complement(52..765)
                     /locus_tag="VFR02_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9870158.1"
                     /translation="MKQVPIAAKIRTTRGSAQTRRLRAEGLVPAEVYGHKETNQSITV
                     VEKDLNKILATSRGENIFFLLNVDGQKGEPALAVIKELQYDKIKSSIIHADFHKVNMN
                     EKIRIKVPIKIVNGDMAEGVKEGGTLQTFMRSLEVYCLPTQIPEAVQVDVASLKVGEA
                     VHVSDLKLAEGVKALQDSGSVIASVAAQVAEEVKAEAAPGAAVAGAPAAAGAAEPEVL
                     TAKKKEEGADAKAPAAEKK"
     gene            complement(808..1770)
                     /locus_tag="VFR02_06675"
     CDS             complement(808..1770)
                     /locus_tag="VFR02_06675"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9870159.1"
                     /translation="MGAKKAKLEGMKVFSGTANPSLAKAIAKSLGVPLGELMVTRFSE
                     GEIRVKINEDVRGRDIFLIQPTCPPVNDNVIELLIMLDAFKRASARRITAVLPYYGYA
                     RQDRKDQPRVPITAKLMANIITVAGTNRLLTMDLHAQQIQGFFDLPVDHLYAYPVLSG
                     YFRNKKLKDLTVVSPDVGGIKMARAYAKGLGADLAIVDKRRTGPNEVEAMNLIGEVKD
                     RTVLIPDDIIATGSSLVEAVNALVKFGAGDIYACCTHAILSGNAVQKLQKTALKEIVV
                     TDTIPLPPEKRVDKIKVLTVAPLLGEAILRIHNERSISTLFDDP"
BASE COUNT          312 a          594 c          649 g          439 t
ORIGIN      
        1 cggcgggccg gggcggcttg gtgaggcatc ggaaggaaaa gggtccggcg gttacttctt
       61 ttcggcggcc ggcgccttgg cgtcggcgcc ttcttccttc ttcttggccg tgaggacctc
      121 gggttcggcg gcgccggcgg cggccggggc gcccgcgacg gcggcgccgg gggcggcctc
      181 cgccttgact tcctcggcga cctgtgcggc cacggaggcg atgacgctcc cgctgtcctg
      241 gagggccttc acgccctcgg ccagcttcag gtcggacacg tggaccgcct cgccgacctt
      301 cagggacgcc acgtccacct ggaccgcttc ggggatctgg gtgggcaggc agtagacttc
      361 cagcgagcgc atgaaggtct gcagggtgcc gccttccttg acgccctcgg ccatgtcgcc
      421 gttgacgatc ttgatgggca ccttgatgcg gatcttctcg ttcatgttca ctttatggaa
      481 gtcggcgtgg atgatcgagc tcttgatctt gtcgtactgc agttccttga tgaccgccag
      541 ggcgggctcg cccttctggc cgtcgacgtt cagcaggaag aagatgttct ccccgcgcga
      601 ggtggccagg atcttgttca ggtccttttc caccaccgtg atggactggt tggtctcttt
      661 gtgcccgtag acttcggcgg gcaccaggcc ctcggcccgc aggcggcgcg tctgggcgct
      721 cccccgggtg gtgcggatct tggcggcgat gggtacttgt ttcatttcaa cacggctcct
      781 cggctgattt aacaggtagt tttggcgtca gggatcatcg aagagggtgc tgatggaccg
      841 ctcattgtgg atccgcagga tcgcttcgcc caacaggggc gccaccgtga gaaccttgat
      901 cttgtcgacc cgcttctcag ggggaagggg aatggtatcc gtaaccacga tttctttcaa
      961 ggccgttttt tgcagttttt gcacggcatt tcccgacaaa atagcgtggg tacagcaggc
     1021 atagatgtcc ccagccccga acttgaccag ggcgttgacc gcctccacca gggagctgcc
     1081 ggtggcgatg atgtcgtccg ggatgaggac cgtgcggtcc ttgacctcgc cgatgaggtt
     1141 catggcctcc acctcgttgg ggcccgtgcg gcgcttgtcc acgatggcca ggtccgcccc
     1201 caggcccttg gcgtaggccc gggccatctt gatgccgccc acgtcgggcg agaccaccgt
     1261 gaggtccttc agcttcttgt tgcggaaata gcccgacaac accgggtagg cgtagaggtg
     1321 gtccaccggc aggtcgaaga agccctggat ctgctgggcg tgcaggtcca tggtgagcag
     1381 gcggttggtg cccgccacgg tgatgatgtt ggccatgagc ttggccgtga tggggacccg
     1441 gggctggtcc ttgcggtcct gccgcgcgta cccatagtag ggcagcacgg cggtgatgcg
     1501 cctggccgag gcgcgcttga aggcgtccag catgatgagc agctcgatca cgttgtcgtt
     1561 gaccgggggg caggtgggct ggataaggaa gatgtcccgg ccccgcacgt cctcgttgat
     1621 cttgacgcgg atctccccct cggagaagcg ggtgaccatc aactcgccca ggggcacccc
     1681 cagggacttg gcgatggcct tggccaggga cgggttggcc gtgccgctga agaccttcat
     1741 gccctccaat ttcgccttct tggcgcccat gccttttccc tcgcttgttt tctcagaccg
     1801 aaattatatc ggctccacgg tcactttcgg aaaaaaacta aggaccggca atgaaattca
     1861 atcggcaatg atgaacgcgg atgaacgccg taaataaatg atgagaattt ctgaacatct
     1921 gcgttcatcg gcgtccatcg atgctaatcg ccttgggttt tgttattgcc tctaaggccg
     1981 gcctttttta tccc
//
LOCUS       DASTJJ010000544         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_76683, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000544 DASTJJ010000000
VERSION     DASTJJ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76683"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFR02_06680"
     CDS             <1..311
                     /locus_tag="VFR02_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9870160.1"
                     /translation="AAELDGASIGRKLVSITLPYLMPVVQVLLIIQVLTTFQIFTEVY
                     VMTNGGPMHSTEVIGTYIYKTAFGSMDLGYASAMAMLMFGVLLVFSVVRLYQLKQVAR
                     "
     gene            311..1243
                     /locus_tag="VFR02_06685"
     CDS             311..1243
                     /locus_tag="VFR02_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9870161.1"
                     /translation="MAPATRPRRPAPAPLPRVRGLFSPQDRRNPRVRLFLGLGTLFLF
                     LGALVTLAPILWMVLGSFRSPEELYAVPPTFLPQDPRFDNYTEALRLMPLARYLANSL
                     FLCGAVVAGQLLFCSMAAYSISKLRIRFAGGLLLLFMTSLMVPFEVLIIPLYLITKNF
                     PLGPGFPGVNLINTYAAVILPSVFSAFGILVLKDFYDSIPNEILYAARIDGCGEWKVF
                     VRFILPMTRPIMAILAVFSFVTTWNSFFWPLVVLNNPDYYPLVLGVQKLIDMGEPWNV
                     VLAALVMSTLPSVALLVLFQKTIIRGIAYTGIYG"
     gene            1240..1821
                     /gene="yqeK"
                     /locus_tag="VFR02_06690"
     CDS             1240..1821
                     /gene="yqeK"
                     /locus_tag="VFR02_06690"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET9870162.1"
                     /translation="MSGKAREREVAAWVRTQVGPARWKHIQGVVQSAEKLARRFGLPV
                     GKARLAAWLHDCAKELPRARMLAWMRGTPFRLDAGERALPALWHPHAGAAIAWKKWGV
                     RDRGLLEAVRCHTLGGERMGPLAQAIFVADFIEPGRRFPGVGEARRAARESLAGGVRA
                     KASQTVALLLSEGRLVHPRLLLTWNAFVKKDRP"
     gene            1818..>2071
                     /locus_tag="VFR02_06695"
     CDS             1818..>2071
                     /locus_tag="VFR02_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870163.1"
                     /translation="MKSWMMAAVLAAAALAGGWWSHRALSREPLPLFLAVESPDELDA
                     FHVGLFLSGAPGVFSPVTLTGPQAQALRDAFDRKDPGPAR"
BASE COUNT          275 a          710 c          682 g          404 t
ORIGIN      
        1 aggccgccga gctggacggg gcctccatcg gccgcaagct ggtgagcatc accctgcctt
       61 acctcatgcc cgtggtgcag gtcctgctca tcatccaggt gctgaccacc ttccagatct
      121 tcacggaggt ctacgtgatg accaacggcg gacccatgca ttccaccgag gtcatcggca
      181 cctacatcta caagacggcc ttcgggtcca tggacctggg ctatgcctcc gccatggcca
      241 tgctgatgtt cggcgtgctg ctggtctttt ccgtcgtccg cctttaccag ctcaagcagg
      301 tggcccgctg atggcccccg cgacacgccc ccgccgtccg gccccggcgc ccctcccccg
      361 ggtgcgcggg cttttctcgc cccaggaccg gcgcaacccc cgggtgcgcc tcttcctggg
      421 cctgggcacc ctgttcctct tcctgggggc gttggtgacc ctggcgccga tcctttggat
      481 ggtgctgggt tccttccggt cgcccgagga gctttacgcc gtgcccccga ccttcctgcc
      541 ccaggacccc cggttcgaca actacacgga ggccctgcgg ctcatgccct tggcgcgcta
      601 cctggccaac agcctcttcc tgtgcggggc ggtggtggcc ggccagctgc tcttttgctc
      661 gatggcggcc tattcgatct ccaagctgcg gatccggttc gccgggggcc tgttgttgct
      721 cttcatgacc agcctgatgg tgccctttga ggtgctcatc atcccgctgt acctcatcac
      781 caagaatttc cccctgggcc cgggattccc cggggtgaac ctgatcaaca cctacgcggc
      841 cgtcatcctg ccctcggtct tcagcgcctt cggcatcctg gtcttgaagg atttctacga
      901 ttccatcccc aacgagatcc tctacgcggc ccggatcgac gggtgcggcg agtggaaggt
      961 cttcgtgcgg ttcatcctgc ccatgacgcg ccccatcatg gccatcctgg cggtcttttc
     1021 cttcgtgacc acctggaaca gcttcttctg gccgctggtg gtcctcaaca acccggacta
     1081 ttacccgctg gtcctgggtg tgcagaagct catcgacatg ggcgaaccct ggaacgtggt
     1141 gctggcggcc ctggtcatga gcacgttgcc ctcggtggcg ctgttggtgt tgttccagaa
     1201 gaccatcatc cggggcatcg cctacacggg gatctacggt tgagcgggaa ggcgcgggaa
     1261 agggaagtgg cggcctgggt caggacccaa gtgggtccgg cgcgctggaa gcacatccaa
     1321 ggcgtggtcc aaagcgccga gaaactggcc cggcgcttcg ggttgccggt gggcaaggcc
     1381 cggttggcgg cctggctgca tgattgcgcg aaggagctgc cccgggcgcg gatgctggcc
     1441 tggatgcggg gcacgccttt tcgattggac gcgggggagc gggccctgcc cgcgctctgg
     1501 catccccacg ccggcgccgc catcgcctgg aagaaatggg gggtccggga ccggggcttg
     1561 ttggaggccg tccggtgcca taccctgggg ggcgagcgca tgggccccct ggcccaggcc
     1621 atcttcgtgg cggatttcat cgagccggga cgccgctttc ccggggtcgg ggaggcccgg
     1681 cgggccgccc gggagagcct ggccgggggc gtgcgggcga aggcatccca gaccgtggcc
     1741 ctcctgcttt ccgaagggcg gctggtccat ccccgcctgc ttctgacgtg gaacgctttc
     1801 gtgaaaaagg atcgcccatg aaaagttgga tgatggcggc cgtcctggcc gccgcggcgt
     1861 tggccggcgg gtggtggagc catcgggcgc tttcccggga accgctgccg cttttcctgg
     1921 cggtggagtc gccggatgaa ttggacgcct tccacgtggg gcttttcctc tcgggcgcgc
     1981 cgggcgtctt cagcccggtc accctcacgg gcccccaggc ccaggcattg cgcgacgcct
     2041 tcgaccgcaa ggacccgggc ccggcccgcc a
//
LOCUS       DASTJJ010000545         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_443227, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000545 DASTJJ010000000
VERSION     DASTJJ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443227"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1129)
                     /gene="mnmA"
                     /locus_tag="VFR02_06700"
     CDS             complement(29..1129)
                     /gene="mnmA"
                     /locus_tag="VFR02_06700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9870164.1"
                     /translation="MAMSGGVDSSVALGLLQEQGHEVVGLTLKILPCEITPGENGAVS
                     VKTVEPKGQRCCSVEDIQDARSVALKLGAPHYVNEGLDVFKANVVDRFLNEYERGLTP
                     NPCVECNRFAKLPLLLKQALALGCEKLATGHYARVDQDPATGRWRLLKALDPDKDQSY
                     VLYKLTQKLLSHLLFPLGETVKARVREDARRFQLNIAEKPESMEICFVTQASYRDFVR
                     IHRPRAFVPGDIVDVSGKVLGRHEGVAGYTVGQRKGLNLSGGPYFVVELNPETATVVV
                     GRDEDTFTSKFTVGEMNWVAWEPLTGPREVMAKIRYRSVEQPAVLYPEGPHRVRVALK
                     DPARAVTPGQSTVFYEGDAVVGGGVIQRPGRA"
     gene            complement(1266..>1953)
                     /locus_tag="VFR02_06705"
     CDS             complement(1266..>1953)
                     /locus_tag="VFR02_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9870165.1"
                     /translation="SPVAEVGEICARKGVAFLVDAAQTAGAVPLHMEALKIDFLAFTG
                     HKGLMGPPGTGGLIVSPKRDLDSFIEGGTGSNSDQETQPTQWPDKFESGTQNYWGLAG
                     LKASVEFLLKTSVQKVRRHEEALTAQFLKEAQRIRGVRLYGLPGEGTAARAAVVSLNV
                     EGRDPAELAYELDERFGIMVRAGLHCAPLAHRTIGTYPTGTVRFSFGYYNTPKDIEKA
                     LAALKALARH"
BASE COUNT          336 a          672 c          603 g          342 t
ORIGIN      
        1 ccgcccgtaa aaccaaaaag acggaggctc aggcccgccc gggcctttgg atgacgcccc
       61 cgcccaccac cgcgtcgccc tcataaaaga ccgtggactg gccgggggtg acggcccggg
      121 cggggtcttt caacgccacc cggacccggt ggggcccttc ggggtacagc acggcgggct
      181 gttccaccga ccggtagcgg atcttggcca tcacttcccg gggcccggtc aggggttccc
      241 aagccaccca attcatctcg cccacggtga acttgctcgt gaaggtgtcc tcgtcccggc
      301 ccaccaccac ggtggcggtc tcggggttga gctccaccac gaaatagggg ccgccggaca
      361 ggttgaggcc cttgcgctgg cccacggtgt agcccgccac gccctcgtgg cggcccaaca
      421 ccttgcccga gacgtccacg atgtcgccgg gcacgaaggc ccggggccgg tggatcctca
      481 cgaaatcccg gtagctggcc tgggtcacga agcagatctc catgctctcg ggcttctcgg
      541 cgatgttgag ctggaagcgc cgcgcatcct cccgcacccg ggccttgacc gtctccccca
      601 gggggaagag caggtgggag aggagctttt gggtgagctt atagaggacg tagctctggt
      661 ccttgtccgg gtccagggcc ttcaagaggc gccagcggcc cgtggccggg tcctggtcca
      721 cccgggcgta atggcccgtg gccagtttct cgcagcccag cgccagggcc tgcttcaaga
      781 gcaggggcag cttggcgaag cggttgcact ccacgcaggg gttgggggtg aggccccgct
      841 catactcgtt gaggaaccgg tccaccacgt tggccttgaa gacgtccagg ccctcgttga
      901 cgtaatgggg cgcgcccagc ttgagcgcca ccgagcgggc gtcctggatg tcctccaccg
      961 aacagcaacg ctggcccttg ggctccacgg tcttgaccga gaccgcgccg ttctcgccgg
     1021 gcgtgatctc gcagggcagg atcttcagcg tgaggcccac cacctcgtgg ccctgttcct
     1081 gcaacaaccc cagcgccacg ctggaatcca cccccccgct catcgccacg ccgatcttca
     1141 taatgaaacc aacccttttc accacaaagt tcacgaagta cacgaagtta aagcttagaa
     1201 ccgaaaaata ctttcggcct tatttagtga actccgtgta cttcgtggta aaaaatctta
     1261 aatcctcaat gtcgtgccaa agccttcaaa gccgccaggg ccttttcgat gtccttcggc
     1321 gtgttgtagt aaccgaagct gaaccggacc gtgccggtcg ggtaggtgcc gatggtgcgg
     1381 tgcgccaggg gggcgcaatg aagccccgcc cgcaccatga tgccgaagcg ttcgtccaat
     1441 tcataggcca gctccgccgg gtcccgtccc tcgacgttca aggacaccac ggccgcccgg
     1501 gcggcggtcc cctcgccggg caggccgtaa agccgcaccc cccggatcct ctgggcttcc
     1561 ttgaggaact gggccgtcag ggcctcctca tgccggcgga ccttctgcac gctggtcttg
     1621 aggaggaact cgaccgacgc cttcaatccc gccaggcccc aataattttg ggtgccgctc
     1681 tcgaacttgt ccggccactg ggtgggttgg gtctcctggt cgctgttgga acccgtgccg
     1741 ccctcgatga aggaatccag gtcccgcttg ggcgagacga tcagcccccc cgtgcccggg
     1801 gggcccatga ggcccttgtg gcccgtgaag gccaaaaagt cgatcttgag ggcttccata
     1861 tggaggggca ccgccccggc ggtctgggcc gcgtccacca aaaaggccac gcccttcctg
     1921 gcgcagatct cccccacctc ggccaccggg gaa
//
LOCUS       DASTJJ010000546         1403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_564562, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000546 DASTJJ010000000
VERSION     DASTJJ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564562"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFR02_06710"
     CDS             <1..959
                     /locus_tag="VFR02_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9870166.1"
                     /translation="WPLGALLLAWTAWSAIALWRDRRDLEALGHQNLVLSPGTAWARR
                     WAKGTFLILALGAAGLGAARLQGGLVPQDWTARGSDLVVVLDVSKSMLTQDVKPSRLD
                     AAKKALEDWLGGLQGDRVGLVVFSGQALVQVPLTLDLDAVSQILDKADTDMVALGGTD
                     LGAGIQTALSAFPKDPKRGKAILLLTDGETTDGTSNVGEACQEAVSQKVPIITVGMGT
                     PQGQPIPDGMGFWGQPSYKQDASGQVHISHLDEGTLRRIAQQTGGVYLQGDSAEALTK
                     IQGTVERLSQTDLAAKGGLKREELSPLCALAAAACLLLSSIL"
     gene            973..>1403
                     /locus_tag="VFR02_06715"
     CDS             973..>1403
                     /locus_tag="VFR02_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870167.1"
                     /translation="MGLKRGVGIGILALLALGRGPAGAEDFLYAPGRWAYDRGDYQKA
                     RDIFQGELQNSPNDADAAYGLGNSLYQLGQYDQASQAYQKCLQDDPKLEAGWYNLGNS
                     LYQQKDYQGAAQSYQKALDLDPKDQDAARNLKLARSKIPPSP"
BASE COUNT          248 a          422 c          504 g          229 t
ORIGIN      
        1 tttggcccct gggggcgctg ctcctggcct ggacggcctg gtccgccatc gcgttgtggc
       61 gcgaccgccg cgacctggag gcgttggggc accagaacct ggtgctgtcc ccgggcacgg
      121 cctgggcccg ccggtgggcc aaggggacct tcctgatcct ggcgctgggg gcggccgggt
      181 tgggggcggc ccgcctgcag ggcggcctgg tcccccagga ttggacggcc cggggcagcg
      241 acctggtggt ggtgctggac gtttccaaga gcatgttgac gcaggacgtg aagcccagcc
      301 ggttggacgc ggccaagaag gccttggagg attggctggg gggattgcag ggggaccggg
      361 tgggattggt ggtcttttcc gggcaagccc tggtgcaggt gcctttgacg ctcgacctgg
      421 acgccgtgtc ccagatcctg gacaaggccg ataccgacat ggtggcgttg ggcggcacgg
      481 acctgggggc cggcatccaa actgcgctct cggcttttcc caaggatccc aagcggggca
      541 aggccatcct gcttttgacc gacggggaaa ccaccgacgg gacgtccaat gtgggggagg
      601 cctgccagga agcggtcagc cagaaggtcc cgatcatcac ggtgggcatg gggacgcccc
      661 agggccagcc catcccggac gggatgggct tttgggggca gccttcttat aagcaggacg
      721 cctccgggca ggtgcacatc agccatttgg acgaggggac attgcggcgc atcgcccaac
      781 agacgggcgg cgtctatctt caaggggaca gcgccgaggc cctgaccaag atccagggga
      841 cggtggaacg gctgtcccag accgacctgg cggccaaggg cggcctgaag cgcgaggaac
      901 tgtcccccct ctgcgccctg gcggcggcgg cctgcctgct gctgtcgtcg atcttgtgag
      961 gagccgggaa ccatgggatt gaaacgggga gtgggcatcg ggatcctggc cctgctggct
     1021 ttggggcggg gcccggcggg ggcggaggat ttcctctacg cgccaggccg ttgggcctat
     1081 gaccgcgggg attaccaaaa ggcccgcgat attttccaag gggaactcca aaactccccg
     1141 aatgacgccg acgcggccta tggattgggc aacagcctgt accagttggg acagtatgac
     1201 caggcttccc aagcctacca gaaatgcctc caagacgatc ccaagctgga ggcggggtgg
     1261 tataacctgg gcaacagcct ttatcaacaa aaggattatc agggagcggc gcagtcctac
     1321 caaaaagcct tggacctgga cccgaaggac caagacgcgg cgcgcaacct taaattggct
     1381 cggtccaaga tcccgccttc ccc
//
LOCUS       DASTJJ010000547         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_564622, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000547 DASTJJ010000000
VERSION     DASTJJ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564622"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..>1330)
                     /locus_tag="VFR02_06720"
     rRNA            complement(115..>1330)
                     /locus_tag="VFR02_06720"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          208 a          497 c          362 g          263 t
ORIGIN      
        1 tgcttactac ttacacctcg ccgctccgcc cgacgctgcg cgtccggccc ccaccgtccc
       61 gcgggggaac ggagcccagc cgccgacgcg gtcgcgaccc accaactgcg gccgtcggaa
      121 atcggcctcg accattagga cggctcggct ccggtcggtt gcccgccgtc cacctgccgc
      181 ctatctaccc cctcgtctcg ggggggtctt tcctctttcg agtggggagg ctcgtcttgg
      241 ggagggattc ccgcttagat gctttcagcg gttcgcccac ccgcacgtgg ctacccagcg
      301 ctgccggttc tccgacaact ggcccaccag cggtgcgtcc accccggtcc tctcgtacta
      361 ggggcagctc cccgcaacct cccatccgcc cgcggcggat agagaccgaa ctgtctcacg
      421 acgttctgaa cccagctcgc gtaccgcttt catgggcgaa cagcccaacc cttgggacct
      481 tctccagccc caggatgcga cgagccgaca tcgaggtgcc aaaccctgcc gtcgatgtga
      541 actcttgggc aggatcagcc tgttatcccc ggggtagctt ttatccgtta agccacggcc
      601 cgtccaccag gcaccgtggg atcactaggc ccgcctttcg gccctgctcg gcctgtccgc
      661 ctcgcagtca agctcccttc gtgcccttac acgctcccgc cgattgccat ccggcgtgag
      721 ggaaccattg ggcgcctccg ttactcttta ggaggcgacc gccccagtca aactccccac
      781 cagtcgcggt ccctggcctg gatgacaggc gcaggtgaga acgacggcac gcgcagagtg
      841 gtatttcaac ggcgcctcca cctgggctag cgccccggct tccccggctc ccacctatcc
      901 tacacaacgc gcgccttcgc ccaacgacaa gttggagtaa agctccacgg ggtctttttg
      961 tcctgccgcg ggtcgacggt atcttcaccg tccctacaat ttcgccgagt cccccgtcga
     1021 gacagcgccc agatcgttgt acctttcgtg cgggtcggaa cttacccgac aaggaatttc
     1081 gctaccttag gaccgttata gttacggccg ccgtttaccg gggcttcggt tcgcagcttg
     1141 caccgctccc cttaaccttc cggcactggg caggtctcag cccctataca tccacacctg
     1201 acgtgttggc agggaccggt gtttttggta aacagtcgcc tgggcctggt cactgcggcc
     1261 ccctcgcgct ccacccgcta agggcttcac gctaccgggg cgccccttct cccaaagtta
     1321 cggggccaat
//
LOCUS       DASTJJ010000548         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_443522, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000548 DASTJJ010000000
VERSION     DASTJJ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443522"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFR02_06725"
     CDS             <1..302
                     /locus_tag="VFR02_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870168.1"
                     /translation="TERFAEPPALPGAADGGESVPKTKWLLPLGLLTLPLWLWLALGL
                     LSPRGQSHGSPDNAPYILAAGVFFLGMGLVLFLIPLISRSGKKGGQKPGGGPPAP"
     gene            356..1588
                     /locus_tag="VFR02_06730"
     CDS             356..1588
                     /locus_tag="VFR02_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9870169.1"
                     /translation="MPVKPRLLVLGTGFGAVSLLKVVDPKRYDVTAVSPRNHFLFTPL
                     LPSTTVGTLEFRSIVEPVRRACRSMRYFQASSRALDPAAKTVRCVSAIDQTAFEVPYD
                     LLVVAVGAKVNTFGIAGVAENALFLKTIDDARRIRRRVIECFEQASQPNLDDDKRRQL
                     LHFVSVGGGPTGVEFAAELNDFVTDELKAAFPALFPLARITVVDAADHILSAFDASLS
                     RFTSDHFKRRNIQVLSKVRVTEVARDSLRLNNGETLRFGLLVWSTGIGPTIAANNFPL
                     PKDKASRILTDPTLKVKGFEDVYALGDCAFVGDQGLPATAQVAMQQGKYLGRALNRLS
                     RGKPPGPFRYHNYGMLAYVGDDAAIADVEGLKSKGKAAFFFWRSAYFTRLLSVKNKIL
                     VLFDWLKTSLFGRDISRL"
     gene            1786..3024
                     /locus_tag="VFR02_06735"
     CDS             1786..3024
                     /locus_tag="VFR02_06735"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9870170.1"
                     /translation="MTAPSKPRLLVLGTGFSSFSLLKKINFDHFDVTVVSPQNYLLFT
                     PLLAGTTVGTLEFRNIIEPLRRSFPRVRYYQAACVGLDTAQKTAACRDSSDASEFSVP
                     YDLLVVGLGAKVNTFGIPGVEENALFLKSIEDARSIRRRILECFECASRPDLSADRRR
                     QLLNFVVMGGGPTGAEFAAELHDLMEREMRAYYAPLMADVRVTIVDVADFILGAFDVS
                     LRDYATRHFAAEGVQVKTGSSALRVEKEKIVFKDGSEMPYGLLVWATGIAPTPAVKAF
                     PLPKDKGSRIQVDDHLRVADHPGVFALGDCAAVAGMPLPATAQVAMQQGEYLAGELNG
                     KIENRDPQPFRYHHLGIMCYVGGDVAIADLPALKWHGHTAHLFWRLAYLMRLMTLKNK
                     CLVFFGWLKTAFFGRDISRI"
     gene            3189..3758
                     /locus_tag="VFR02_06740"
     CDS             3189..3758
                     /locus_tag="VFR02_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870171.1"
                     /translation="MITPTHAVYNLALLGRRDRPDRTWPILLGAFFPDLPGTFCIFYS
                     FVIQGRSLERIHEVIYPSAFWQGWADAFHSIPLALLLGAACLLLKWAPGAWFAASAVL
                     HSLEDFPVHDANPHRHFWPLADWRFQSPLSCYGPGGHVAWVAAADFCLLAWAAWVLWR
                     RGLPAWGKGLVGTALLLEAAHSILAVLKG"
BASE COUNT          616 a         1399 c         1157 g          744 t
ORIGIN      
        1 tcaccgaacg tttcgccgag ccccccgccc tgcccggggc ggcggacggc ggcgaaagcg
       61 tccccaagac caaatggctg ctgcccctgg ggctgctcac gctcccgctc tggctgtggc
      121 tggccttggg gctcttgtcc ccccgcggcc aaagccacgg gtccccggac aacgccccct
      181 atatcctggc cgcgggcgtc tttttcctgg gcatgggcct ggtgcttttc ctgatccccc
      241 tcatttcccg gtccggcaaa aaagggggcc aaaaacccgg cgggggcccc ccggcgcctt
      301 gaaatcgccc cccggggccg ttaccttcgg accatcccct gttcgacgag gccccatgcc
      361 cgtgaaaccc cgcttgttgg tccttggcac cggcttcggc gccgtcagcc tgctcaaggt
      421 ggtcgacccc aaacgctacg acgtgaccgc cgtgagcccc cgcaaccatt tcctcttcac
      481 gccgctgctc ccctccacca cggtgggcac gctggagttc cgcagcatcg tggaacccgt
      541 gcggcgggcc tgccggtcca tgcgctactt ccaggcctcc agccgggccc tggaccccgc
      601 ggccaagacg gtgcgctgcg tgagcgccat cgaccagacc gccttcgagg tgccctacga
      661 cctcttggtg gtggccgtgg gggccaaggt caacaccttc ggcatcgcgg gcgtggccga
      721 gaacgccctc ttcctcaaga ccatcgacga cgcccggcgc atccgccggc gggtcatcga
      781 gtgctttgag caggcctccc aacccaacct ggacgacgac aagcgccgcc agctgctgca
      841 cttcgtgagc gtgggcggcg gccccacggg cgtggaattc gccgccgagc tcaacgactt
      901 cgtcaccgac gagctgaagg ccgccttccc ggccctcttc cccctggcgc gcatcacggt
      961 cgtcgacgcc gccgaccaca tcctcagcgc cttcgacgcc tccctcagcc gcttcaccag
     1021 cgaccacttc aagcgccgca acatccaggt gctttccaag gtgcgggtca ccgaggtggc
     1081 gcgggacagc ctgcggctga acaacgggga gacgctgcgc ttcggcctgc tggtctggtc
     1141 cacgggcatc ggccccacca tcgcggccaa caacttcccc ctgcccaagg acaaggcctc
     1201 ccgcatcctg accgacccca ccctgaaggt gaagggcttc gaggacgtct acgccctggg
     1261 cgactgcgcc ttcgtgggcg accagggcct gcccgccacc gcccaggtgg ccatgcagca
     1321 gggcaagtac ctgggccgcg ccctcaaccg gctctcccgg gggaagccgc ccgggccctt
     1381 ccgctaccac aattacggca tgctggccta cgtgggggac gacgcggcca tcgccgacgt
     1441 ggaggggctc aagtccaagg gcaaggccgc cttctttttc tggcgctcgg cctatttcac
     1501 ccggctcttg agcgtgaaaa acaagatcct ggtgctcttc gactggctca agacctccct
     1561 tttcggccgg gacatcagcc ggctctaacg ggccttgccg cgtcgcttgg cttgggggcc
     1621 ccggcgccct ttttcccggc ttccgctccc tttttttagg ggaaaaaccg ccccggatta
     1681 ggtatgctct tgcctggttt caccccggcc gtccctttca ccctgttttt caacggtttt
     1741 cgccgcgccc aatttgttgg accctggtcc acgagaggaa aaagcatgac ggccccatcc
     1801 aagccccggc ttttggtgtt aggcaccggt ttttcctcct tcagcctgtt gaaaaagatc
     1861 aatttcgacc atttcgacgt gaccgtcgtc agcccccaga actacctgct cttcacgccg
     1921 ctcctggccg gcaccacggt gggcacgctg gaattccgca acatcatcga gcccctgcgc
     1981 cgttccttcc cccgggtccg ctattaccag gccgcctgcg tggggctgga cacggcccaa
     2041 aagaccgccg cctgccggga cagctcggac gcatccgagt tttccgtccc ctatgacctg
     2101 ctggtggtgg gcctgggcgc caaggtgaac accttcggca tcccgggggt ggaggagaac
     2161 gccctgttcc tcaagagcat cgaggacgcc cggagcatcc gccgccgcat cctggaatgc
     2221 ttcgagtgcg cctcccggcc cgacctgtcg gccgaccggc gccgccagct cctgaatttc
     2281 gtggtcatgg gcgggggccc cacgggggcc gagttcgccg ccgaactgca cgacctgatg
     2341 gagcgggaga tgcgggccta ctacgccccc ctgatggccg acgtccgggt gaccatcgtg
     2401 gacgtggccg acttcatcct gggggccttc gacgtttccc tgagggacta cgccacccgg
     2461 catttcgcgg ccgaaggcgt gcaggtcaag accggctcca gcgccctgcg cgtcgagaag
     2521 gaaaagatcg tcttcaagga cgggtccgag atgccctacg ggctcctggt ctgggccacg
     2581 ggcatcgcgc ccacgccggc cgtcaaggcc ttccccctgc ccaaggacaa gggctcccgc
     2641 atccaggtgg acgaccacct gcgggtggcg gaccacccgg gcgtcttcgc cctgggcgac
     2701 tgcgccgccg tcgccggcat gccgcttccc gccacggccc aggtggccat gcagcagggc
     2761 gagtatttgg cgggcgaatt gaacgggaag atcgagaacc gcgaccccca gcccttccgc
     2821 taccaccact tgggcatcat gtgctacgtg gggggcgacg tggccatcgc cgacctgccg
     2881 gccctcaagt ggcacggcca tacggcccat ctcttctggc gcctggccta cctcatgcgg
     2941 ctcatgaccc tgaagaacaa atgcctggtc tttttcggct ggctcaagac cgccttcttc
     3001 ggccgggaca tcagccgcat ctgatccctc gagcccctca ttccttttta caatgataac
     3061 ggctgatcaa ttccgatgaa gccttccctt ccgcaaaatg ccccagtaaa gacaaaattc
     3121 atttgttttg atttttcgga ctcatcaagt ttcatcattc ttcatcactg ttcatggacc
     3181 ggactttcgt gatcaccccc acccacgcgg tctacaacct ggcgctcctg ggccgccggg
     3241 accggccgga ccggacctgg cccatcctgc tgggcgcctt tttccccgac ctgccgggca
     3301 ccttctgcat cttttattcc ttcgtgatcc agggccggtc gctggagcgg atccacgagg
     3361 tgatctatcc ttccgccttc tggcaggggt gggcggacgc cttccattcc atccccctgg
     3421 cgcttttgct gggcgcggcc tgcctgctcc tcaagtgggc gccgggcgcc tggttcgccg
     3481 caagcgccgt cctgcattcc ctggaggact ttcccgtgca cgacgcgaac ccccaccggc
     3541 atttctggcc gctggcggac tggcgcttcc aaagccccct gtcctgctat gggccgggcg
     3601 ggcacgtggc ctgggtggcg gcggcggact tttgcctctt ggcctgggcg gcctgggtcc
     3661 tatggcggcg ggggctgccg gcttggggaa aagggctggt ggggacggcg ctgctgctgg
     3721 aagccgccca ttccatcctg gcggtcttga agggctgatg gaaaatcatt taagaaccga
     3781 caacaaaatt cgatcggcaa tgatgaacgc agatgaacgc cgtaaagaaa tgatgagaat
     3841 ttctgaatat ctgcgttcat cggcgtccat ctacgctaat tgccttgggt tttgttacag
     3901 cctctaagaa ccatgt
//
LOCUS       DASTJJ010000549         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1055505, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000549 DASTJJ010000000
VERSION     DASTJJ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055505"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="VFR02_06745"
     CDS             complement(<1..1681)
                     /locus_tag="VFR02_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9870172.1"
                     /translation="MARPLYLDALKEKVLIFDGAMGTNIQTYNLTKEDFGGKEGLNDY
                     LVVTKPQVIREIHEGFLKAGVDALETNTFGSNRLKLAEYGIADQIVEVNTAAVKLARE
                     LADQYSTPDHPRFVVGSMGPTGMLPASEDPDLGKITVDELEAIYYEQACIMIDAGVDA
                     LLVETSQDILEMRAAVFGCHRARTEKGSQVAIQAQTTLDTTGRMLLGTDIGSVLTTFS
                     RLPIDVLGMNCSTGPKEMKDSVRYLSQYCPFPISTIPNAGLPENRGGQAVYPLSPEAL
                     AAALKEFVEEFGVGVVGGCCGTTHEHLRQVVRAIGKQKPKQRAITHACQVSSMIKSVS
                     MDQDPKPLIVGERVNSQGSRKIKQMLLADDYEGMVAVGRGQAEGGAHVLDVCVALTER
                     ADEKDQMKKVVKKLALGVDSPLMIDSTEAEVIEEALKIYPGRALINSINLEGDGGRVK
                     RVCPLAKKYGAAVVAMTIDDLYYDKSKPAEKGMAKTAARKLEVAKKITDICVNEYGLP
                     AEDIVFDPLTFTLATGEEEFRNSAVETMEGIRAIKKEIPGVRTVLGLSNVSF"
     gene            complement(1753..>1944)
                     /locus_tag="VFR02_06750"
     CDS             complement(1753..>1944)
                     /locus_tag="VFR02_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9870173.1"
                     /translation="HNAGRGAKYTRTRLPVALAYARRAGDRSAALRREYALKQKDRGE
                     KLRMAAAWRRGRGRRARKP"
BASE COUNT          291 a          631 c          629 g          393 t
ORIGIN      
        1 cgaaggacac gttggaaagg cccaggacgg tgcgcacgcc cgggatctcc ttcttgatgg
       61 cccggatgcc ttccatggtc tccacggcgg agttccggaa ctcctcctcg ccggtggcca
      121 gggtgaaggt cagggggtcg aagacgatgt cctcggcggg caggccgtat tcgttcacgc
      181 agatgtcggt gatctttttc gccacttcca gcttgcgcgc ggccgtcttg gccatgccct
      241 tctcggcggg tttggacttg tcgtaataaa ggtcgtcgat ggtcatggcc accaccgcgg
      301 cgccgtactt cttggccagg gggcagaccc gcttcacgcg gccgccgtcg ccttccagat
      361 tgatggaatt gatgagcgcc cggcccggat agatcttcag ggcttcctcg atgacctcgg
      421 cctcggtgga gtcgatcatg agcgggctgt ccacgcccag ggccagcttc ttgaccacct
      481 tcttcatctg gtccttctcg tcggcccgct cggtcagggc cacgcagacg tccagcacgt
      541 gggcgccgcc ctccgcctgg ccacgcccca cggccaccat gccctcgtag tcgtcggcca
      601 ggagcatctg cttgatcttg cgcgatccct gggagttgac ccgctcgccc acgatcaggg
      661 gcttggggtc ctggtccatg gaaacgctct tgatcatgga cgagacctgg caggcgtggg
      721 tgatcgcccg ttgcttgggc ttttgtttgc cgatggcccg caccacttgc cgcaggtgct
      781 cgtgggtggt gccgcaacag ccgcccacga cccccacgcc gaattcctcg acgaattcct
      841 tcaaggccgc cgccaacgcc tcgggcgaaa gggggtagac cgcctgcccg ccccggttct
      901 cgggcagccc cgcgttgggg atggtggaga tggggaaggg gcaatattgg ctcaggtacc
      961 ggaccgaatc cttcatttcc ttggggccgg tggagcagtt catgcccaag acgtcgatgg
     1021 gcaggcgcga gaaggtggtg agcaccgaac cgatgtccgt tcccaagagc atgcggcccg
     1081 tggtatccag cgtggtctgg gcctggatgg cgacttggga gcccttttcg gtcctcgccc
     1141 ggtggcagcc gaagaccgcc gcgcgcatct ccaaaatatc ctggctggtc tccaccaaca
     1201 gcgcgtccac gcccgcgtcg atcatgatgc aggcctgttc gtaatagatg gcctccagct
     1261 catccaccgt gatcttgccc aggtccgggt cttcggaagc cggcagcatg cccgtggggc
     1321 ccatggaccc caccacgaaa cggggatggt cgggggtgga atactggtcc gccagttccc
     1381 gggccagctt cacggcggcg gtgttgacct cgacgatctg gtccgcgatg ccgtattcgg
     1441 ccagcttgag ccggttggac ccgaaggtgt tggtctccaa agcgtccacg cccgccttca
     1501 ggaagccctc gtggatctcc cggatgacct ggggcttggt gacgaccaag tagtcgttca
     1561 ggccttcctt gccgccgaag tcctccttgg tcaggttgta ggtctggatg ttggtgccca
     1621 tggccccgtc gaaaatgagg accttttctt tgagggcgtc caggtagagc ggtcgggcca
     1681 agggttgcct tctttattta atgtcggaca agcgtccgga gcgccacagt ttacggtctt
     1741 agggacgggg cttcaaggtt tacgggcccg ccggccccgc ccccggcgcc aagccgcggc
     1801 catccgcagt ttttcccccc ggtccttttg cttgagcgcg tattcccgcc tcaacgcggc
     1861 ggaacggtcc cccgcccggc gggcataggc cagggccacg ggaaggcggg tccgggtgta
     1921 cttggccccc cggcccgcgt tgtg
//
LOCUS       DASTJJ010000550         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_200196, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000550 DASTJJ010000000
VERSION     DASTJJ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200196"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1021
                     /gene="hemG"
                     /locus_tag="VFR02_06755"
     CDS             <1..>1021
                     /gene="hemG"
                     /locus_tag="VFR02_06755"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9870174.1"
                     /translation="TPLLSPLGKLRAALEPFRPRRRDPQDESVGAFVRRRLGREVFEN
                     LAQPLLGGVYNADPDDLSLSAAFPRFQEMERVQGSLIRALASRPPAARTSGARYSLFV
                     SLKRGMGSLIEALVDRLPAGTLRLESPVVSVRHRDLGPWELDLGSGKMETDVLVLALP
                     PHAMRPLIGHLDTEWDKLLLGVPAHDSATLNLGFRREDVGHPLDGFGFVVPAREGRSI
                     LGCTFSSQKFEGRAPEGKVLLRAFLGAQAAGEMKALGEAAMLEKVLGELGPILGLRGR
                     PLLTHTAFYAKAMSHFRPGHLSLASRIAQKAQETPGLFLAGNGFQGVGIPDCVAAGLA
                     AAEKV"
BASE COUNT          148 a          374 c          335 g          164 t
ORIGIN      
        1 ccacgccgct gctttcccca ctcggaaaac tgcgcgccgc gctggaaccc ttccggcccc
       61 gccgccggga cccccaggat gagagcgtgg gcgccttcgt gcggcgccgc ctgggacggg
      121 aggtcttcga gaacctggcc caacccttgc tcgggggcgt ctacaacgcc gacccggacg
      181 acctgagcct ctccgccgcc ttcccccgct tccaggaaat ggaaagggtc cagggttccc
      241 tgatccgggc cctggcttcc cgcccccccg cggcccggac ctccggcgcc cgctattcgc
      301 tcttcgtcag cctgaagcgg ggcatggggt ccctgatcga agccctggtg gaccgccttc
      361 ccgccggcac cctgcgcttg gaaagccccg tggtgtccgt ccgccaccgg gacctgggcc
      421 cctgggaact ggacctgggc tccgggaaga tggagaccga cgtcctggta ttggccctgc
      481 cgccccacgc catgcggccc ctcatcgggc acctggatac cgagtgggac aaactgctcc
      541 tgggcgtccc cgcccatgat tcggccaccc tgaacctggg gttccggcgg gaggacgtgg
      601 ggcatccctt ggacggtttc ggtttcgtgg tgccggcccg ggaaggccgg tcgatcctgg
      661 gctgcacctt ctccagccaa aaattcgaag gccgcgcgcc cgagggcaag gtgctgttga
      721 gggccttcct gggggcccag gccgccggag agatgaaggc cctcggggaa gccgccatgc
      781 tggaaaaagt gttgggggaa ttgggcccca tcctgggttt gaggggaagg cccctcctga
      841 cccacacggc cttttacgcc aaggccatgt cgcacttccg gcccgggcac ctgagcctgg
      901 cctcccgcat cgcccagaag gcccaggaaa cccccggcct cttcctggcc ggcaacggtt
      961 tccaaggcgt gggcatcccg gattgcgtgg ccgcaggcct cgccgccgcc gaaaaggtga
     1021 t
//
LOCUS       DASTJJ010000551         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1422283, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000551 DASTJJ010000000
VERSION     DASTJJ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422283"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFR02_06760"
     CDS             complement(<1..481)
                     /locus_tag="VFR02_06760"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9870175.1"
                     /translation="MARTPIIAGNWKMYKTPQESVDFARQLAGKLKDVQGREVLVCPT
                     FLSAAAVVDALRFSGIQVGAQDAHWEDEGAYTGNVSPAMLKAAGLAYAILGHSERRQY
                     DNDTDDRVNRKLKNVLKNGLRAILCVGETLQEREAGRTEAVIEGQLKGGLADISLADL
                     "
     gene            complement(613..1815)
                     /locus_tag="VFR02_06765"
     CDS             complement(613..1815)
                     /locus_tag="VFR02_06765"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9870176.1"
                     /translation="MEYRKLTVEDLAPKGKRVLVRVDFNVPLDASLKITDDTRIRESL
                     KTIQYLSDKGARVVLVSHLGRPKGVDEKLRLKPVAGRLAELLKKPVAALADCVGPEVE
                     KAVAAMKDGEVALLENVRFHAAEEKNDPAFAKQLAALADLYVNDAFGTAHRAHASTEG
                     VTKFVAQSAAGFLMMKEIQYLGGALSDPARPFVAIVGGSKVSSKIDVIKSLMKVVDVL
                     IIGGGMAYTFLKARKFPIGNSIVENDKMDLAKELMGRSLDTDVPLLLPIDHVIADKYD
                     AKANIKTVPRGGILEGWEGLDVGPNTIEKYATFIRTAKTIFWNGPVGVFEIEPFAKGT
                     LAIAKLLAEASDRGAITIVGGGDSVAAVTQMGLADKMSHISTGGGASLEFMEGKQLPG
                     IVALTDKK"
     gene            complement(1834..2772)
                     /locus_tag="VFR02_06770"
     CDS             complement(1834..2772)
                     /locus_tag="VFR02_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9870177.1"
                     /translation="MEGNPVVSGPLFFILLVGAVIYVARAVKIVRPFEKGLIERLGRF
                     AKTAEPGLTVLFPPFENMRKVDMREQVIDVPPQEVITKDNVVVTVDAVIYFHVTDAFK
                     VVYNVARFELAALKLAQTNLRNTIGELDLDQTLNSRERINTQLREILDEATDKWGVKV
                     TRVEIQKIDPPADIVNAMSAQMKAERTKRAAIIEAEGIKQAAITKAEGEKQSAILEAE
                     GQAGAIERVASANKFQKIALAEGEAQAVTTVFKAIHEGNPTQDLLALKYMEMLGNVAD
                     GQATKIFLPLEASGTLGALSTIAEAFKTDLKSQPKK"
     gene            complement(2803..3237)
                     /locus_tag="VFR02_06775"
     CDS             complement(2803..3237)
                     /locus_tag="VFR02_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9870178.1"
                     /translation="MVPAIWWGLLAVSLFIAEVATASFFFLWIGAGAAVTALASFFVG
                     PDWAQCLVFLLTSIFLVAVSRPWAARISPKTARLANVDSLVGQEGRIARPLRAPGQAY
                     AVVEGESWRVEEASGKSLKAGDRVKVREVRSNQLIVELQNAG"
     gene            complement(3260..4267)
                     /gene="gap"
                     /locus_tag="VFR02_06780"
     CDS             complement(3260..4267)
                     /gene="gap"
                     /locus_tag="VFR02_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9870179.1"
                     /translation="MAIKVGINGFGRIGRNVYRAAMGNPNIEIVAVNDLTDSKTLAHL
                     LKYDSVLGNLHADIKAAEGAILVNGKEIKVFSQRDPAQIPWGTVGAEFIVESTGFFTD
                     KEKAKAHLRDSVKKVIISAPATNEDITIVLGVNEDKYDPKAHHVVSNASCTTNCLAPF
                     AKVLHEKFGIVKGLMTTIHSFTNDQVTLDFPHKDLRRARAASLNMIPTSTGAAKAIGL
                     VMPEMKGKLDGLSIRVPTPVVSVVDLVAEVSKTVTKEDVNNALKEAAKSAKLDRYLDV
                     TEEPLVSMDFKGNPHSSIVDAQSTYVTGGNMVKVLSWYDNEWGYSNRVVDLVEYMASK
                     K"
     gene            complement(4394..>4982)
                     /locus_tag="VFR02_06785"
     CDS             complement(4394..>4982)
                     /locus_tag="VFR02_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET9870180.1"
                     /translation="PVPAGAFEGSARPRCADCGEGDYALEETLAFGAYQGPLRLAHHA
                     FKFEGMERLGARLADRMAGEVPAGFWEGAAALVPVPMSAERERERGYHPARLLARRLS
                     ARAGLPLGDWLRKVRPTPPQMSLDRAKRLTGPKGAFRYAGPSPGPEAVVLVDDVLTTG
                     ATLEECARVLRRAGWARIRAVVFGRTPRDFRPAGP"
BASE COUNT          788 a         1639 c         1616 g          939 t
ORIGIN      
        1 ccaggtcggc cagcgagatg tcggccaagc cgcccttcaa ctgcccctcg atgaccgcct
       61 cggtcctccc cgcttcccgt tcctgcaggg tctcgcccac gcacaagatg gcccgcaggc
      121 cgttcttcaa cacgttcttg agcttgcggt tgacgcggtc gtcggtgtcg ttgtcgtact
      181 ggcggcgttc cgaatggccc aggatggcgt aggccaggcc cgcggccttc agcatggcgg
      241 ggctcacgtt gcccgtatag gccccctcgt cctcccaatg ggcgtcctgg gcgcccacct
      301 ggattcccga gaaacgcagc gcgtccacca ccgccgccgc cgaaaggaag gtgggacaga
      361 ccaggacttc ccggccctgg acgtccttca gtttccccgc cagttggcgg gcgaagtcca
      421 cggactcttg gggggttttg tacatcttcc agttgccggc aatgatgggg gtgcgggcca
      481 tgtcgtgttt cctttcaagg tgtcaacaaa ggcggataca tatttaccac gaagtccact
      541 aagttcacga agtggaacca aacgaagatc ttttcctgaa cttcgtgcgc tccgtgaact
      601 tcgtggtgaa aattatttct tatcggtgag ggccacgatc ccgggcagct gcttgccttc
      661 catgaactcc agggaggcgc cgccgccggt ggagatgtgg gacatcttgt ccgccaggcc
      721 catctgggtg accgccgcca ccgaatcgcc gccgcccacg atggtgatgg cgccccggtc
      781 cgaggcctcg gccagcagct tggcgatggc cagggtgccc ttggcgaagg gctcgatctc
      841 gaagacgccc acggggccgt tccaaaagat ggtcttggcc gtgcggatga aggtggcgta
      901 cttctcgatg gtgttggggc ccacgtccag gccttcccag ccctccagga tgccgccccg
      961 gggcacggtc ttgatgttgg ccttggcgtc gtacttgtcc gcgatcacgt ggtcgatggg
     1021 caaaagcagg ggcacgtcgg tgtcgaggga ccggcccatg agctccttgg ccaggtccat
     1081 cttgtcgttc tccacgatgg aattgccgat cgggaacttg cgggccttca ggaaggtgta
     1141 ggccatgccg ccgccgatga tgagcacgtc caccaccttc atgaggctct tgatgacgtc
     1201 gatcttggac gagaccttgg agccgcccac gatggccacg aaaggccgcg ccgggtcgga
     1261 cagcgccccg cccaggtact ggatctcctt catcatgagg aaccccgcgg cggactgggc
     1321 cacgaacttg gtcacgccct cggtggaggc gtgggcccgg tgggccgtgc cgaaggcgtc
     1381 gttcacgtag aggtcggcca gggccgcgag ctgcttggcg aaggcggggt cgttcttttc
     1441 ctcggccgcg tggaagcgca cgttctccaa cagcgccacc tcgccgtcct tcatggccgc
     1501 cacggccttt tccacctcgg ggcccacgca atccgccagg gccgccacgg gcttcttcag
     1561 gagttcggca aggcgtcccg ccacgggctt caaccgcagc ttctcgtcca cgcccttggg
     1621 ccgccccagg tgcgagacca gcaccacccg ggcccccttg tccgagaggt actggatggt
     1681 cttgagcgat tcccggatgc gggtgtcgtc ggtgatcttg agcgaggcgt ccagcggcac
     1741 gttgaaatcc acccggacca acacccgctt tcccttgggc gccaggtcct cgacggtgag
     1801 tttacggtat tccacggaat cctccttgac ggtttacttt ttgggttggg acttcaggtc
     1861 ggtcttgaag gcctcggcga tggtcgagag cgcacccagg gtgccgctgg cctccagggg
     1921 caggaagatc ttggtggcct ggccgtcggc cacgttgccc agcatctcca tgtacttgag
     1981 ggccagcagg tcctgcgtgg ggttcccctc gtggatggcc ttgaagaccg tggtgacggc
     2041 ctgggcctcg ccctcggcca gggcgatctt ttggaactta ttggcgctgg ccacgcgctc
     2101 gatggcgccc gcctggccct cagcctccag gatggcggat tgtttctcac cctcggcctt
     2161 ggtgatggcg gcctgcttga tgccctccgc ctcgatgatg gcggcgcgtt tggtgcgttc
     2221 ggccttcatc tgggcgctca tggcgttcac gatgtcggcg ggcgggtcga tcttctggat
     2281 ctccacgcgc gtcaccttga cgccccactt gtcggtggcc tcatccagga tctcccgcag
     2341 ctgggtgttg atgcgttccc ggctgttgag ggtctggtcc aggtccaact cgccgatggt
     2401 gttgcgcagg ttggtctggg ccagtttgag ggccgccagc tcgaagcgcg ccacgttgta
     2461 gaccaccttg aaggcgtccg tcacgtggaa atagatgacc gcgtccaccg tgaccaccac
     2521 gttgtccttg gtgatgacct cctggggcgg cacgtcgatg acctgctcgc gcatgtccac
     2581 cttgcgcatg ttctcgaagg gcgggaaaag gacggtcaat ccgggctcgg cggtcttggc
     2641 gaaacggccc agcctttcga tcaagccctt ctcgaagggg cggacgatct tgacggcccg
     2701 ggccacgtag atgaccgcgc ccaccagcaa aatgaagaac aaaggcccac tcaccaccgg
     2761 gttcccctcc atcaccgacc tccttgggcc gcgtccacgg ccctaccctg cgttttgaag
     2821 ttcaacgatc aattggttgg acctgacttc ccggaccttc acccggtcgc cggccttcag
     2881 gctcttgccc gaggcctctt ccacccgcca ggattccccc tccaccacgg cataggcctg
     2941 gccgggggcg cgcagggggc gggcgatccg gccttcctga cccaccaggg aatccacatt
     3001 ggccaaccgg gcggtctttg gggagatccg ggcggcccaa gggcggctga cggccaccag
     3061 gaaaatggaa gtgaggagga agaccaggca ttgggcccag tccgggccca cgaaaaagct
     3121 ggccagcgcg gtgacggccg cccccgcgcc gatccacagg aagaaaaagg aggcggtggc
     3181 gacctcggcg atgaacaggc tcaccgccaa caacccccac cagatcgccg gaaccatggt
     3241 cccccccgct gcggcccgat tacttcttcg aggccatgta ctccacgagg tcgaccacgc
     3301 ggttcgagta gccccactcg ttgtcgtacc aggacagcac cttcaccatg ttgccgcccg
     3361 tcacgtaggt ggactgggcg tcgacgatgg aggaatgcgg gttgcccttg aagtccatgg
     3421 agaccagggg ctcctcggtc acgtccaggt agcggtccag cttggcgctc ttggccgctt
     3481 ccttgagggc gttgttgacg tcctccttgg tgacggtctt ggagacctcg gccaccaggt
     3541 cgaccaccga caccaccggg gtcggcacgc ggatggacaa cccgtccagt ttgcccttca
     3601 tctcgggcat gaccaacccg atggccttgg cggcccccgt gctggtgggg atcatgttca
     3661 ggctggccgc gcgggcgcgg cgcaggtcct tatgcgggaa gtcgagcgtg acctggtcgt
     3721 tggtgaagct gtggatggtg gtcatcaggc ccttcacgat gccgaacttc tcgtgcagca
     3781 ccttggcgaa gggggccagg cagttggtgg tgcaggaggc gttggacacc acgtggtgcg
     3841 ccttggggtc gtacttgtcc tcgttcacgc ccagcacgat ggtgatgtcc tcgttggtgg
     3901 ccggggccga gatgatgacc ttcttcacgc tgtcgcgcag gtgggccttg gccttctcct
     3961 tgtcggtgaa gaagccggtg gattccacga tgaattcggc gcccacggtc ccccagggga
     4021 tctgggccgg gtcgcgctgc gagaagacct tgatctcctt gccgttgacc aggatggcgc
     4081 cctcggcggc cttgatgtcg gcgtgcaggt tgcccagcac cgagtcgtac ttcaacaggt
     4141 gggccagggt cttggaatcg gtcaggtcgt tgaccgcgac gatctcgatg ttggggttgc
     4201 ccatcgccgc gcggtacacg ttgcgcccga tgcggccgaa tccgttgatg ccgactttga
     4261 tcgccatgct gttcctccgg ttttatccaa ataataaagg gttcgatcgc ccccggaagg
     4321 ggctgaaatg aagcgctaag ctaacacagg gggtgggcag ggtcaaggcg gaaagccagc
     4381 attgaggccg ctttcagggg cctgcggggc ggaaatccct tggggtgcgg ccgaagacca
     4441 ccgcccggat ccgggcccat cccgcgcgcc gtaggacccg ggcgcattcc tccagggtgg
     4501 ctccggtggt cagcacgtca tccaccaaga ccaccgcctc gggccccggc gaagggcccg
     4561 cgtaacggaa ggcgcccttt gggcccgtca aacgcttcgc gcggtcaagc gacatctggg
     4621 gcggcgtggg ccggaccttg cgcagccaat cccccagggg caatcccgcc cgcgccgaaa
     4681 ggcgccgggc cagcagacgg gccggatggt aaccccgttc cctttcccgt tccgcgctca
     4741 tgggcacggg caccagggcc gccgcgcctt cccagaaccc cgccgggacc tccccggcca
     4801 tgcggtccgc caagcgcgcg cccaaccgct ccatcccctc gaacttgaag gcgtggtgcg
     4861 ccaagcgcag ggggccttgg taggcgccga aggccaaggt ctcctccaat gcgtagtccc
     4921 cctccccgca atccgcgcag cggggccgcg ccgagccctc gaaggcgccc gcggggaccg
     4981 gc
//
LOCUS       DASTJJ010000552         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1544877, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000552 DASTJJ010000000
VERSION     DASTJJ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544877"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="ilvC"
                     /locus_tag="VFR02_06790"
     CDS             complement(<1..557)
                     /gene="ilvC"
                     /locus_tag="VFR02_06790"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003329283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9870181.1"
                     /translation="MPKIYYEQDADLNVLKGKTIAVIGYGNQGRNQALNLKDSGVKVI
                     VGARSEKTKAQAKADGIEAFDAKDAAAKADIIQILIQDDLQGSLYKNDILPGLKAGKA
                     IMFSHGFNIHFGQIQPPEDIDVFMIAPKGPGHLVRTTYVDGKGVPALVAIHQNATGNA
                     MKLALAYGKAIGASRAGILETTFKE"
     gene            complement(732..1274)
                     /gene="ilvN"
                     /locus_tag="VFR02_06795"
     CDS             complement(732..1274)
                     /gene="ilvN"
                     /locus_tag="VFR02_06795"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET9870182.1"
                     /translation="MRHTISVLVNNHAGVLSRVSNLFSARGYNIDSLVVGVTDNPQVS
                     RITLVVKGDERIIDQVEKQLNKLIDVIQVIDLTHDDFIARDLALIKVKAEAHTRSQVM
                     EIVQCFDGKIVDVALKSFIIEVTGKESKINAMVELLSNYGILELVRTGEVAISRGKKV
                     AEEEGSKVKVKAGKAGQSNL"
     gene            complement(1300..3069)
                     /gene="ilvB"
                     /locus_tag="VFR02_06800"
     CDS             complement(1300..3069)
                     /gene="ilvB"
                     /locus_tag="VFR02_06800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET9870183.1"
                     /translation="MAMKSKKMKTGAQMVVDILIDEGVTMAWGVTGGAILPVYDAMFL
                     NRDKIKLIDTRHEQGAVHMAEGYAKSTGKVGVCVVTSGPGATNIVTGLADAYMDSVPL
                     VAITGQVPTSLIGTDGFQEADVVGITRSCTKHNYLVKNIQELPQIVRDAFFIARTGRP
                     GPVLIDIPKDIAAASAEVEIPDEPSIPLSKFVPTPEKGQVDKIAELLKQAKRPLIYAG
                     GGVINAGASEELYKFARKTNIPVTLTLMGLGAYPATDRQFIDMLGMHGSYRANMALTH
                     CDLLIAIGSRFDDRVTGKLSEFSPHSKKVHIDVDPTSIGKNVKVDAHVLGDMRSILKD
                     LTKKAGTKEYDEWYHQLESWNHKHPLAYKQDPKGPTLPQFAIDEIYRITKGDAYVATE
                     VGQHQMWAAQYYKFNKPRRWLTSGGLGTMGFGFPAAIGAAFAHPGATVVDIAGDGSFQ
                     MTLQELAIVKEYELNVKTIVINNKFMGMVKQWQDLFFDKRYAATSIPAQPDFVKLADA
                     YGIAGYRAETPGETTKVLEKALLTKGPCLVDVVVDAEEHVYPMVPAGGAVKDIVLSKA
                     HQIEKMMAAKADKGPKKPTQVHL"
BASE COUNT          477 a          964 c         1014 g          699 t
ORIGIN      
        1 tcttccttga aggtggtctc caagatgccc gcccggctgg cgccgatggc cttgccgtag
       61 gccagggcca gcttcatggc gttgcccgtg gcgttctggt ggatggcgac cagggccggc
      121 acgcccttgc cgtccacgta ggtggtgcgc accaggtggc ccgggccctt gggggcgatc
      181 atgaacacgt cgatgtcctc ggggggctgg atctggccga aatggatgtt gaagccgtgg
      241 gagaacatga tggccttgcc ggccttcagg cccggcagga tgtcgttctt atagaggctc
      301 ccctgcaggt cgtcctggat caggatctgg atgatgtcgg ccttggcggc ggcgtccttg
      361 gcgtcgaagg cctcgatgcc gtcggccttg gcctgggcct tggtcttttc cgaacgcgcg
      421 cccacgatga ccttgacgcc cgagtccttg aggttcaggg cctggttgcg gccctggttg
      481 ccgtaaccga tgaccgcgat ggtcttgccc ttcaaaacgt tcaggtcggc gtcttgttcg
      541 tagtagatct tcggcatttc catctcctta atttttgttc aagaaaacca tttcaccacg
      601 agccccctga tagcgcacaa cgccgggggc cactaagttc actaagacaa caagaatcat
      661 ttttgaatca aaagtcttcc tttgtgccct ttgtggcgcc aaaggcgctt gtggttcaaa
      721 tgtcctgact tctacaggtt cgactggccg gccttgcccg ccttcacctt caccttcgaa
      781 ccttcctctt cggccacctt cttgccccgg gaaatggcca cttcgccggt gcggaccagc
      841 tccaagatgc catagttgct caagagctcc accatggcgt tgatcttgct ctccttgccc
      901 gtgacctcga tgatgaagct cttcagggcc acgtccacga tcttgccgtc gaagcactgc
      961 acgatctcca tcacctggct gcgggtgtgg gcctcggcct tgaccttgat gagcgccagg
     1021 tcgcgggcga tgaagtcgtc atgggtcagg tcgatgacct ggatcacgtc gatgagcttg
     1081 ttgagctgct tctcgacctg gtcgatgatg cgctcatcgc ccttcaccac cagcgtgatg
     1141 cgcgagacct gggggttgtc ggtgacgccc accaccaggc tgtcgatgtt gtatcccctc
     1201 gccgagaaga ggttggacac gcgggaaagc acccccgcgt gattgttcac caagacggat
     1261 atggtatgtc gcatggcctt gcctttcgcg tttccggggt taaaggtgga cttgggtggg
     1321 ctttttgggg cccttgtcgg ccttggccgc catcatcttc tcgatctggt gggccttgga
     1381 gagcacgatg tccttcaccg cgccgcccgc gggcaccatg ggatagacgt gttcctccgc
     1441 gtccaccacc acgtccacca ggcaggggcc cttggtcagc agggcctttt ccagcacctt
     1501 ggtggtctcc ccgggcgtct cggcccggta gcccgcgatg ccgtaggcgt ccgccagctt
     1561 gacgaagtcc ggctgggccg ggatcgacgt ggccgcgtag cgcttgtcga agaacaggtc
     1621 ctgccactgc ttcaccatgc ccatgaactt gttgttgatg acgatggtct tcacgttcag
     1681 ctcgtattcc ttcacgatgg ccagttcctg cagggtcatc tggaagctgc cgtcgcccgc
     1741 gatgtccacc accgtggcgc cggggtgggc gaaggccgcc ccgatggccg ccgggaaccc
     1801 gaagcccatg gtgcccaacc cccccgaggt cagccaacgc cggggcttgt tgaacttgta
     1861 gtactgggcc gcccacatct ggtgctggcc cacctcggtg gccacgtagg cgtcgccctt
     1921 ggtgatgcgg tagatctcgt cgatggcgaa ctggggcagg gtcggcccct tggggtcctg
     1981 cttataggcc agcgggtgct tgtggttcca ggattccagc tggtggtacc actcgtcgta
     2041 ttccttggtg cccgcctttt tggtcaggtc cttgaggatg gagcgcatgt cgcccagcac
     2101 gtgcgcgtcc accttgacgt tcttgccgat ggaggtcggg tccacgtcga tgtgcacctt
     2161 cttggaatgg ggcgagaact cgctgagctt gcccgtcacc cggtcgtcga agcggctgcc
     2221 gatggcgatc agcaggtcgc aatgggtcag ggccatgttg gcacggtagg agccgtgcat
     2281 gcccagcatg tcgatgaact ggcggtcggt ggccgggtag gcccccaggc ccatgagggt
     2341 cagcgtcacc gggatgttgg tcttgcgggc gaacttgtac agctcctcgg acgccccggc
     2401 gttgatgacc ccgccgccgg cgtagatgag cgggcgcttg gcctgcttca gcagctcggc
     2461 gatcttgtcg acctggccct tctcgggcgt aggcacgaac ttggacagcg ggatggaggg
     2521 ctcgtcgggg atctccacct cggcggaggc cgccgcgatg tccttgggga tgtcgatgag
     2581 gaccggcccg gggcgccccg tgcgggcgat gaagaaggcg tcgcgcacga tctggggcag
     2641 ctcctggatg ttcttgacca ggtagttgtg cttggtgcag gagcgggtga tgcccaccac
     2701 gtcggcttcc tggaaaccgt cggtgccgat gagcgacgtg ggcacctggc ccgtgatggc
     2761 gaccaggggc acggagtcca tgtaggcgtc ggccaggccg gtgacgatgt tggtggcgcc
     2821 cgggccggag gtcaccacgc agacgcccac cttgccggtg gacttggcgt agccttcggc
     2881 catgtgcacc gcgccctgtt cgtggcgggt gtcgatgagc ttgatcttgt cgcggttgag
     2941 gaacatggcg tcgtagaccg gcaggatggc cccgcccgtc acgccccagg ccatggtgac
     3001 cccctcgtcg atgaggatgt ccaccaccat ctgggccccg gtcttcattt tcttcgattt
     3061 catcgccatg atcgtctcct taggttctgg ggaaggcccc cacgcgaaag agtgtgattt
     3121 tacaataact tccgcgtttt tcgaggcttt atcc
//
LOCUS       DASTJJ010000553         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1788477, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000553 DASTJJ010000000
VERSION     DASTJJ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788477"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="xseA"
                     /locus_tag="VFR02_06805"
     CDS             <1..1132
                     /gene="xseA"
                     /locus_tag="VFR02_06805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9870184.1"
                     /translation="SCAFFKGQNFGLAFQPATGQKVIVGGNLSLFEARGQFQLIVKSM
                     EPAGLGALQLAFEQLKKKLMAEGLFDGERKKPVPEHPHRVALITSPTGAAIQDFMKIL
                     ETRVGIDEVELFPVKVQGVEAPAEIIGAIEAVSRRAEHDVLVITRGGGSLEDLAAFND
                     EGVVRALADCSIPTVCAVGHEIDFSLCDFVADRREPTPTAAAAHLAPGRDETRDQLDS
                     LAHRLSEAMDGLLEGKRRDLEGLLTSLQDRRPSLLLDQARQTLDYQSEKLRGALEDRL
                     KERGADLERLSGFLARTTPLQLMKPLKARLDGLEAQLKAYHPHGPLKRGYAIVTSKKT
                     GKILRSPDDVKKGERLVIEVEKGKLESQVIQADADAGQATLF"
     gene            1145..2026
                     /locus_tag="VFR02_06810"
     CDS             1145..2026
                     /locus_tag="VFR02_06810"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9870185.1"
                     /translation="MTEIRKGVTSIGFAGLGLLGGAIALRLKEQGWPVTAWNRTQGKA
                     ARLGLPEASSLQDLVSANEIVMSCLANDEAAKAVYLGPQGVFSTPKPGLIVLEMSTLS
                     PQTSLELHAKARSLGVRMLDLPVLGSTPAVKAGAITLVAGGDKALFDACGPIYASLAK
                     QWFLMGPEGSGARMKLVVNLMLGVGMEALAEGIALGKGLGLDFETLLAVLSKTAVVAP
                     AFAGKFEKIQKGDYSPQFPLRLMDKDLGLVLKAAEGAGLSLPAAQATRGVTGEVMKQK
                     ADEDLSVIAPFVAKREK"
     gene            complement(2063..>2483)
                     /locus_tag="VFR02_06815"
     CDS             complement(2063..>2483)
                     /locus_tag="VFR02_06815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9870186.1"
                     /translation="RPLGLLYVDLDHFKSINDQYGHSAGDLVLKQTAERMANVLRYVD
                     TVVRMGGDEFAVVLPEVDAESVRYVALRLFQAITRTPMPLEQLGHQDKIIGVTMGLAC
                     LTQPTEDHQVLIRLADQSLYEAKKAGRNRLGALLTDS"
BASE COUNT          451 a          786 c          831 g          415 t
ORIGIN      
        1 ctcctgcgcc tttttcaagg gccagaactt cgggctcgcc ttccaacccg ccacgggcca
       61 aaaggtcatc gtgggcggga acctcagcct ctttgaggcg cggggccagt tccagctcat
      121 cgtcaagagc atggagccgg cggggttggg cgccctgcaa ttggccttcg agcagctcaa
      181 gaaaaaactc atggccgagg ggctctttga cggggaacgc aagaagcccg tgcccgagca
      241 cccccaccgg gtcgcgctca tcacctcgcc cacgggcgcg gccatccagg acttcatgaa
      301 gatcctcgag acccgggtgg gcatcgacga ggtggagctt ttcccggtca aggtgcaggg
      361 ggtggaagcc ccggcggaga tcatcggggc catcgaggcg gtgtcccgcc gggccgaaca
      421 cgacgtgctg gtgatcaccc ggggcggggg aagcctggag gacctggcgg ccttcaacga
      481 cgagggcgtg gtccgggccc tggccgactg ttccatcccc acggtctgcg cggtggggca
      541 tgagatcgac ttcagtttgt gcgatttcgt ggccgaccgg cgcgaaccca cccccacggc
      601 cgcggccgcc cacctggccc cgggccggga cgagacgcgg gaccaactgg attccctggc
      661 ccaccggttg tcggaggcca tggacggcct gctggaaggg aagcgccgcg acctggaggg
      721 cctcttgacc tcgctgcagg accgccgccc cagcctgctc ttggaccagg cgcggcagac
      781 gctggactac caaagcgaga aactgcgcgg cgccctggag gaccggttga aggaacgggg
      841 cgccgacctg gagcgcctgt cgggcttcct ggcccgcacc acgccgctgc aattgatgaa
      901 gcccctaaag gcccgattgg acggcctgga ggcccaattg aaggcctacc atccccacgg
      961 ccccctgaag cggggctacg ccatcgtgac cagcaagaag acgggcaaga tattgcgctc
     1021 gccggacgat gtgaagaagg gcgagcggtt ggtcatcgag gtcgagaagg gcaagctgga
     1081 atcccaagtg atccaagccg atgcagacgc agggcaagcc actttatttt gattcgaggt
     1141 ttacatgact gaaatacgca aaggggtaac ctccatcggc ttcgcggggt tgggcctgct
     1201 gggcggggcg attgcccttc gcctgaagga acaaggctgg ccggtgacgg cctggaaccg
     1261 cacccaagga aaggccgccc ggttggggct gcctgaggct tccagcctcc aggacttggt
     1321 ctccgccaac gagatcgtca tgtcctgcct ggccaacgac gaggcggcca aggcggtcta
     1381 tctggggccc caaggcgtct tctcaacccc caagccgggc ctcatcgtgc tggaaatgag
     1441 caccctctcc ccccagacca gcctggaatt gcacgccaag gcccggtctt tgggggttcg
     1501 tatgttggac ctgcccgtcc tgggttccac cccggcggtc aaggcagggg ccattacctt
     1561 ggtggcgggc ggggataagg ccctttttga cgcctgcggg cctatttacg cctccctggc
     1621 caaacaatgg ttcctcatgg gcccggaagg ctcgggcgcc cgcatgaagc tggtggtgaa
     1681 cctgatgctg ggcgtgggca tggaagccct ggccgaaggg atcgccttgg gcaagggctt
     1741 gggattggat ttcgagaccc tgttggccgt gctgtccaag accgccgtgg tggccccggc
     1801 cttcgcgggc aagttcgaga agatccaaaa aggggattac tcgccccaat ttcccttgcg
     1861 gctgatggac aaggacttgg gactggtcct gaaagccgcc gagggggccg gcttgtccct
     1921 acccgccgcc caggcgaccc ggggcgtgac cggggaagtg atgaagcaaa aggcggacga
     1981 ggacctgtcg gtgatcgcac cttttgtcgc gaagcgggaa aaataagcct tcgccgtcgg
     2041 agaaattccg aaagacccga cttcaactat ccgtcagcaa ggcccccaag cggttgcggc
     2101 cggccttctt ggcctcgtag aggctctggt cggccaggcg gatgagcacc tggtggtcct
     2161 cggtgggctg ggtcaggcag gccagcccca tggtgacccc gatgatcttg tcctggtgcc
     2221 ccaactgttc cagcggcatg ggcgtgcggg tgatggcctg gaagagccgc agggccacat
     2281 agcgcacgct ttcggcgtcc acctcgggca gcaccaccgc gaactcatcc ccgcccatgc
     2341 gcaccaccgt atccacgtac cgcaagacgt tggccatgcg ttcggccgtc tgcttcaaga
     2401 ccaggtcccc ggccgaatgg ccgtattggt cgttgatgga cttgaagtgg tccaggtcca
     2461 cgtagagcaa ccccaagggc cgg
//
LOCUS       DASTJJ010000554         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1422453, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000554 DASTJJ010000000
VERSION     DASTJJ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422453"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFR02_06820"
     CDS             <1..924
                     /locus_tag="VFR02_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870187.1"
                     /translation="DNPEFFEPFRVRETILGQQFKSFLDVGTGPHTAFWAGLFADHRS
                     AQDTAFPVRPILSFVYHTGTSKGVLGTLDTVDRHGLIEPMEVTTLELTRPIEYGLQWV
                     QQDGFIRLDSVLDWQALLAPGQSELFDYGGSAILPVFGDLALMGQLHGYHAGGAQYAV
                     PVWNNLAAGLGFKWQADPEQKGKTFLEVLGLTSKTTNLAGYPGPVQGYGLYVKGSYSP
                     DDAWEFFALYWLGRDYYSKEGDSNYDSVGTDGSYYQAERHYEELGVRRRVAIEAGVTF
                     DFELRSHWIEDSWANSLRLLATVPFDVPVRD"
     gene            937..2235
                     /gene="mtaB"
                     /locus_tag="VFR02_06825"
     CDS             937..2235
                     /gene="mtaB"
                     /locus_tag="VFR02_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET9870188.1"
                     /translation="MTPTVKKKVAFATLGCRVNQYDTQALRESLGADFETVPFEHSAD
                     VYVINTCTVTAAADAEGRQLVRRAKAGNPAAFVVVTGCLAEDRPRETAALPGVDLVLG
                     NREKNLLADRLLGRVSAAPPGPWGAGISRFDGHQRATVKVQDGCNFGCAFCLIPRVRG
                     RMTSRPGDLVLAEARRLAEGGVKELVLAGIQLSSYGRDWGQKASEPRLAPLVGELLRV
                     PGIRRVRLSSYAVADFEDALLPLWAEDAGLCPHLHLPLQSGDEGVLKAMRRPYSLEDY
                     RRTVEKARRAAPRLGLTGDILAGFPGETDQAFRNTLDRIRELGFADFHPFPYSDRPDT
                     PGEAMGPKVPAAVIRERMDALLALKAELNRRSAEAARDRVFPLVVERHGPRTSAGLTD
                     EGLRVLFPLVEGGLGREVAVRVVSFQEGRAWGRVGETGCA"
     gene            2232..>2697
                     /locus_tag="VFR02_06830"
     CDS             2232..>2697
                     /locus_tag="VFR02_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870189.1"
                     /translation="MRSRLTLAALLGCLAASPLWAETADDYYREAAQFYAQKDYDHAF
                     DAYQGAANLDPNPYRAFAGMGNCEYARGHKSKAMEDYRLSLKMYPNNPVLSQFVERLR
                     LELDSKGGPFDKGRIAFGGKNYRLAEKYFQEAVQDDPENLPAFYYQAYCDYLL"
BASE COUNT          418 a          927 c          921 g          431 t
ORIGIN      
        1 gacaaccccg agttcttcga gcccttccgc gtgcgggaga ccatcctggg ccagcagttc
       61 aagagcttcc tggacgtggg cacgggcccc catacggcct tctgggccgg cctcttcgcc
      121 gaccaccgtt cggcccagga caccgcgttc cccgtgcggc ccatcctgtc cttcgtgtac
      181 cacaccggga cctccaaggg cgtcctgggc accctggaca ccgtggaccg ccacggcctg
      241 atcgaaccca tggaagtgac cacgctggag ttgacccggc ccatcgagta cgggttgcag
      301 tgggtccaac aggacggttt catccgcctg gacagcgtcc tcgactggca ggccctcttg
      361 gccccgggcc aaagcgagct tttcgactac ggcggctcgg ccatcctgcc ggtcttcggc
      421 gacctggcgc tgatgggcca actgcacggc taccacgccg gcggcgccca gtacgccgtg
      481 ccggtctgga acaacctggc ggcggggttg gggttcaagt ggcaggccga tccggagcaa
      541 aagggcaaga ccttcctgga ggtcctgggc ctcaccagca agaccaccaa cctggccggc
      601 tatccgggcc cggtgcaggg ttatgggctt tacgtgaagg gctcctattc ccctgacgac
      661 gcctgggaat tcttcgccct ctattggctg ggccgggact attactccaa ggaaggcgac
      721 tccaactacg attcggtggg caccgacggg tcctattacc aggccgagcg gcattatgag
      781 gaactgggcg tgcggcgccg ggtggccatc gaggcgggcg tgaccttcga cttcgaactg
      841 cgctcgcatt ggatcgagga ttcctgggcc aattccctgc ggctcttggc caccgtgccc
      901 ttcgacgtcc ccgtccggga ttgaaggact gaattgatga cgccaaccgt gaaaaagaag
      961 gtggccttcg ccaccctggg gtgccgggtg aaccagtacg acacccaggc cctccgggag
     1021 agcctggggg cggatttcga gaccgtgccc ttcgagcact ccgcggacgt ctacgtcatc
     1081 aacacctgca cggtcaccgc ggccgccgac gccgagggcc ggcagttggt gcgccgggcc
     1141 aaggcgggca accccgccgc cttcgtggtg gtgaccggct gcctggccga ggataggccc
     1201 cgggaaacgg ccgccctgcc cggcgtggac ctggtgctgg gcaaccggga gaagaacctc
     1261 ctggccgacc gcctgttggg ccgggtttcg gcggccccgc ccggcccctg gggcgccggc
     1321 atctcccgct tcgacggcca ccagcgcgcc acggtcaagg tgcaggacgg ctgcaatttc
     1381 ggctgcgcct tctgcctgat cccccgggtg cggggccgca tgacctcccg gcccggggac
     1441 ctggtgctcg ccgaggcccg ccgcctggcg gagggcggcg tgaaggaact ggtgctggcg
     1501 ggcatccagc tctcctccta cgggcgcgat tggggccaaa aagcctccga gccccggttg
     1561 gcccccctgg tgggggagtt gctccgggtg cccggcatcc ggcgcgtgcg gctctcctcc
     1621 tacgcggtgg ccgacttcga ggacgccctg ctgcccctct gggcggagga cgcgggcctc
     1681 tgcccccacc tgcacctgcc gctccaaagc ggggacgagg gggtgctgaa ggccatgcgc
     1741 cggccctatt cgctggagga ttaccgccgg acggtcgaaa aagcccgccg ggcggcgccc
     1801 cggctggggt tgaccggtga tatcctggcc gggttccccg gggagacgga ccaagccttc
     1861 cggaacaccc tggaccggat ccgggaactg ggattcgccg acttccatcc cttcccctat
     1921 tcggacaggc cggacacgcc cggcgaggcg atgggcccca aggtgccggc ggcggtcatc
     1981 cgggaacgca tggacgcgct gctggccttg aaggccgagc tcaaccgccg cagcgccgaa
     2041 gccgcccggg accgggtctt ccccttggtg gtggaacggc acggcccccg gacgtcggcg
     2101 gggctgaccg acgagggttt gcgcgtgctg ttccccctgg tggaaggggg gctggggcgg
     2161 gaagtggcgg tgcgggtggt atctttccag gaaggccggg cctggggccg ggtcggggag
     2221 acggggtgcg catgagatcg agactgacgc tggccgccct cctgggatgc ctggccgcct
     2281 cgcccctctg ggccgagacg gcggatgact attaccggga agcggcccag ttctacgccc
     2341 agaaggatta tgaccacgcc ttcgacgcct atcagggcgc ggccaacctg gaccccaacc
     2401 cctaccgggc cttcgccggc atgggcaact gcgagtacgc ccggggccac aagagcaagg
     2461 ccatggagga ttaccggctc tccctcaaga tgtaccccaa caacccggtc ttgtcccagt
     2521 tcgtggagcg gctgcgcctg gagctggatt ccaagggcgg gcccttcgac aagggccgca
     2581 tcgccttcgg cggcaagaac taccgcctgg cggaaaagta tttccaggaa gccgtccagg
     2641 acgaccccga gaacctgccg gccttctatt accaggccta ttgcgactac ctgctgc
//
LOCUS       DASTJJ010000555         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_688597, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000555 DASTJJ010000000
VERSION     DASTJJ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688597"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFR02_06835"
     CDS             <1..876
                     /locus_tag="VFR02_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9870190.1"
                     /translation="QLTQTIQQMHGATEEVSSTIQNISKGAEEQAAKVQDAFNIIDKM
                     AGNIQTSAEQLSSAEKAVSRSKKASEAGTEAVQKSTAQMNAIHEVVQASAREVNELGA
                     RSKAIGKVVDVITHIADQTNLLALNAAIEAARAGEYGRGFSVVAEEVKKLADGSAEAA
                     NQIAGMIRQIQKEISLVVSSMGTGAEKVTEGLGVVSGAGEALAEIQSASNEVVSQVQD
                     ISASFENQADQASRVVEAITNIVAVSEENASATEEISASTEELTASMESMVAAARQLE
                     GVADRLRSASAKFGD"
     gene            1109..>1228
                     /locus_tag="VFR02_06840"
     CDS             1109..>1228
                     /locus_tag="VFR02_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HET9870191.1"
                     /translation="MIEVKDLVKQYGPFAAVDKITFSVPKGTILGLLGPNGAGK"
BASE COUNT          254 a          369 c          420 g          185 t
ORIGIN      
        1 cagttgaccc agaccatcca gcagatgcac ggggccaccg aggaggtgtc ctccaccatc
       61 cagaacatct ccaagggggc cgaggaacag gcggccaagg tccaggacgc cttcaacatc
      121 atcgacaaga tggcgggcaa catccagacc tcggccgagc agctgtccag cgccgagaag
      181 gccgtgtccc ggtccaagaa ggcctcggag gccggcacgg aagccgtgca gaaatccacg
      241 gcccagatga acgccatcca tgaggtggtg caggcctcgg cccgggaagt gaacgaattg
      301 ggggcccggt ccaaggccat cggcaaggtg gtggacgtca tcacccacat cgccgaccag
      361 accaacctgt tggccctcaa cgcggccatc gaggccgccc gggcggggga atatgggcgc
      421 ggcttttccg tggtggccga ggaggtcaag aaactggcgg acgggagcgc ggaggccgcg
      481 aaccagatcg ccggcatgat ccgccagatc caaaaagaga tctccctggt ggtcagcagc
      541 atggggacgg gggcggaaaa ggtgaccgag gggctgggcg tggtgtcggg ggccggggag
      601 gccctcgccg agatccaaag cgcctccaac gaggtggtga gccaggtcca ggacatttcg
      661 gcctccttcg agaaccaagc cgaccaggcc tcccgggtgg tggaggccat caccaacatc
      721 gtggcggtgt ccgaggagaa cgcctcggcc accgaggaga tctccgcctc caccgaggag
      781 ttgaccgctt ccatggaatc catggtggcg gccgcccggc agttggaggg ggtggcggac
      841 cgtctgcggt cggcctcggc gaaattcggc gattgacgcg acccttccga aagggcccgt
      901 gcgtccataa aaataggccc ggatccccgg gaaccggttg aatcgttaaa tcataaggat
      961 ggggcccatc cgccggcctt ggaggccggg gaaaggatgt gcttgggttt gtcgggtttc
     1021 gggttgaaaa agcgccggat gctcctatag tcggtttccg ccgtccgccg gtggacgcct
     1081 aacattccaa acgactcgag gacttttcat gatcgaagtc aaggatctgg tgaagcaata
     1141 cggccccttc gcggccgtgg acaagatcac cttttcggtc cccaagggga ccatcctggg
     1201 gctgctgggt cccaacggcg ccggcaag
//
LOCUS       DASTJJ010000556         1348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_810255, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000556 DASTJJ010000000
VERSION     DASTJJ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810255"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1348)
                     /locus_tag="VFR02_06845"
     CDS             complement(<1..>1348)
                     /locus_tag="VFR02_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9870192.1"
                     /translation="HTPAWRLEGLPPGRVLQADLLVDANLDHLLKTLGPKTVFNCVAY
                     GAYPFETRADWIHQTNFNLTAKILERLVPEDLACYAHSGTSSEYGDKASGPAETELPE
                     PNSDYAVSKVAAANLIRYYGKKKGLPCANLRFYSIYGPMEDSSRLVPALVREGLAGKY
                     PPLAGPDISRDFVYVEDACEAFVDTALNLKPADYGDSFNIGSGRKTTLREAATAAGKV
                     FGIRQEPAFQAYPNRAWDLAGWFADAGKAKRTLGWEAKVPFEEGLRRTADWYRSLPDP
                     ERYRKASKQFGLDGRYSLSAVVACYKDGQAIPIMHRRLTETFQKLGVEYEIIFVNDNS
                     PDDSEAVITALSRQDRRVLGISHSRNFGSQAAFRSGMEAATKNACVLLDGDLQDPPEL
                     IEQLVGKWKEGYDVVYGRRVKRETSWPMQVAYKAFYWLFNKFSYLPIPRDAGDFSL"
BASE COUNT          219 a          419 c          464 g          246 t
ORIGIN      
        1 atgagcgaaa agtcgcccgc gtcccggggg atgggcaggt aggagaactt attgaagagc
       61 caataaaagg ccttgtaggc gacctgcatg ggccaggagg tctcccgctt cacgcgccgg
      121 ccatagacca cgtcgtagcc ttccttccat ttccccacca gttgctcgat gagctcgggc
      181 gggtcctgca ggtcgccgtc cagcagcacg caggcgttct tggtggccgc ctccatgccg
      241 ctgcggaagg ccgcctggga cccgaaattg cgcgaatggg agatgcccaa gacccgccgg
      301 tcctgccggg acagggccgt gatgaccgcc tcgctgtcgt ccgggctgtt gtcgttgacg
      361 aagatgatct cgtattccac gcccagcttt tggaaggtct cggtcaggcg ccggtgcatg
      421 atggggatgg cctggccgtc cttgtagcag gccaccacgg ccgagaggct gtaacgcccg
      481 tccaggccga attgcttgga agccttgcgg tagcgttccg ggtcgggcag ggaccggtac
      541 caatcggcgg tgcggcgcag tccctcctca aagggcacct tggcttccca gcccaaggtc
      601 cgtttggcct tccccgcgtc ggcgaaccac ccggccaggt cccaggcccg gttcggatag
      661 gcctggaagg ccggttcctg ccggatgccg aagaccttcc ccgcggccgt ggccgcttcc
      721 cgcagggtgg tcttgcggcc gctgccgatg ttgaaggaat cgccgtagtc ggcgggtttc
      781 aggttgagcg cggtgtcgac gaaggcctcg caggcgtcct ccacgtaaac gaaatcccgt
      841 gaaatgtccg ggcccgccag gggcgggtat ttccccgcca ggccttcccg gaccagggcc
      901 ggcaccagcc gggacgaatc ctccatcggg ccgtagatcg aatagaagcg caggttggcg
      961 cagggcaggc ccttcttttt gccgtaatag cggatcaggt tggccgccgc caccttggag
     1021 acggcgtaat cgctgttggg ctcgggcagt tcggtctcgg cgggcccgga ggccttgtcg
     1081 ccgtattcgg acgaggtgcc tgaatgcgcg tagcaggcca ggtcctccgg gaccaggcgt
     1141 tccaggatct tggcggtcag gttgaagttg gtctggtgga tccaatcggc gcgggtctca
     1201 aagggatagg ccccgtaggc cacgcagttg aagacggtct tgggtcccag ggtcttcaac
     1261 agatggtcca ggttggcgtc caccaaaagg tcggcctgca ggacccgccc gggcggaagc
     1321 ccttccaacc gccaggcggg ggtgtgga
//
LOCUS       DASTJJ010000557         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_565797, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000557 DASTJJ010000000
VERSION     DASTJJ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565797"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFR02_06850"
     CDS             <1..293
                     /locus_tag="VFR02_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870193.1"
                     /translation="TNTPVPPILLWPNPFNPDFAVNGELKISGLQPGDKVSFYTVSGE
                     LVAKAAQTLSGALVTWDGRNPQGQMSSAGIYYYLVQRGEKVLLRGKLLIVHQ"
     gene            328..2274
                     /locus_tag="VFR02_06855"
     CDS             328..2274
                     /locus_tag="VFR02_06855"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9870194.1"
                     /translation="MTTIPAPSLPRVLLLLAALAAPGAAFAQGAGSTSTNFLKIGMGA
                     RASAMGGSFTAVSDDATAIFWNPAGLILAGGTQFSLTHAEWLDGVDSEFFAFTQNLDM
                     DGAFGGSLSYLGSGAFPSTLETSTGQYAGLGGMVSASDFAGSAAYAQRLGRWFGGDFF
                     QRSLVGLRATVVGQNVQQFGSGGFSVDLGYLYELERHKFYVGATLLNVGTSIQNYGQP
                     LSYQFGASYKLTKLLDKKDYLLFAAGTTGYVDTGLKFNLGTEYKLRFGKDSLALRAGY
                     STVNNDLGSLSGATLGVGLTHQFAGMQAGLDYAFVPYGVLGDTHRITLNLLTPGGEAP
                     PTASIAARPEFYPGRQTLKVALNSVSEEPLTGWRVAVTDPDGKVVKMFSGKGAPPADV
                     VWDGLGPNGPVPEGRYQMVFQVKDDDDQMSESNPKPVYVRQIHIPKKVPFTYSQSIAG
                     DLLFNSGQDVLMERGYNSIQQAVEAIQKKYPDSWITIIGHTDNQPVAPGGKYKDNQEL
                     SLGRAEAVKDYLTQSGIDPRRLSVKGMGDTQPIASNDTAEGRAKNRRVELQVTGETIA
                     TAADLIAQGKVFYNSKDFKGALERFQAAIEAEPKDAEAYRQAGTCYLRLGDKADAVAA
                     FRQAYQLSPDPQLKAWLDQYGKAN"
     gene            complement(2271..>2941)
                     /locus_tag="VFR02_06860"
     CDS             complement(2271..>2941)
                     /locus_tag="VFR02_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9870195.1"
                     /translation="EALIFDFDGLILDTEMPDFSAWRETYADYGLEFPLDRWALRVGT
                     VASLFDPYAHLESSLGRALPRDEVLRRQRAPNLELVAREVPRPGVVEYLDDAYRMKLR
                     VAVASSSTQAWVVGHLSRLGLADYFHSIRCRDDVAKVKPDPELYELTLETLGIAPSQA
                     LAFEDSHNGIKAAKAAGIFCVAVPNDLTRRLDLDSAGADLRIGSMGDMKLRDLLREAE
                     RTGA"
BASE COUNT          509 a         1020 c          956 g          456 t
ORIGIN      
        1 ctacgaacac gcccgtgccg cccatcctgt tgtggccgaa ccccttcaac cccgatttcg
       61 cggtcaacgg cgaactgaag atctcgggcc tgcaaccggg ggataaggtg tccttctaca
      121 ccgtttcggg cgagttggtg gcgaaggcgg cccagaccct gtccggggcg ctggtcactt
      181 gggacggccg caacccgcag ggccagatgt cctcggccgg catctactac tacctggtcc
      241 agcgcggcga gaaggtgctg ctccgcggca aactgctcat cgtccaccaa taacgtccgt
      301 tcgatgccag gaaccaagac ccgacccatg accaccatcc ccgccccgag cctcccgcgc
      361 gtcctgttgc tgttggcggc cctggcggcc ccgggggcgg ccttcgccca aggcgcgggc
      421 agcaccagca ccaacttcct caagatcggc atgggcgccc gggcctcggc catggggggc
      481 agtttcacgg cggtgtccga cgacgccacg gccatctttt ggaaccccgc ggggctcatc
      541 ctggccggcg gcacccagtt ctccctcacc catgccgagt ggctggacgg ggtggacagc
      601 gagttcttcg ccttcaccca gaacctggac atggacggcg ccttcggcgg cagcctgtcc
      661 tacctggggt cgggcgcctt ccccagcacc ttggagacct ccacgggcca gtacgcgggc
      721 ctggggggta tggtgagcgc ctcggatttc gcgggcagcg cggcctacgc ccagcgcctg
      781 ggccgctggt tcggcgggga ttttttccag cgctccctgg tcgggttgcg ggccacggtg
      841 gtgggccaga acgtccaaca gttcggctcg ggcggcttct cggtggacct gggctacctc
      901 tatgaactgg aacgccacaa gttctacgtg ggcgccaccc tgctcaacgt cgggacctcg
      961 atccagaact acggccagcc cctcagctat cagttcggcg cctcctataa actgaccaaa
     1021 ctcttggaca agaaggacta cctgcttttc gccgccggca ccacgggcta cgtcgatacc
     1081 ggcttgaagt tcaacctggg caccgaatac aagctgcgtt tcggtaagga cagcctggcc
     1141 ctgcgggcgg gctattccac cgtgaacaac gacctggggt cgctgtcggg cgccaccctg
     1201 ggggtgggcc tcacccacca gttcgccggg atgcaggccg ggttggacta cgccttcgtg
     1261 ccctacggcg tgctgggcga cacccaccgc atcaccttga acctgctgac ccccggcggc
     1321 gaagcgccgc cgacggcctc catcgccgcc cggcccgagt tctacccggg ccgccagacc
     1381 ttgaaggtgg ccctcaactc ggtcagcgag gaacccctga ccggttggcg ggtggccgtc
     1441 acggacccgg acggcaaggt ggtgaagatg ttctcgggca agggcgcccc gcccgccgac
     1501 gtcgtctggg acggcttggg ccccaacggc cccgttcccg aggggcgcta ccagatggtg
     1561 ttccaggtga aggacgacga cgaccagatg tccgagtcca accccaaacc ggtctacgtg
     1621 cgccagatcc acatccccaa gaaggtgccc ttcacctaca gccagtccat cgcgggcgac
     1681 ctgctcttca attcgggcca ggacgtgctg atggagcggg gctacaattc catccagcag
     1741 gccgtggagg ccatccagaa gaaatatccg gacagctgga tcaccatcat cggccacacc
     1801 gacaaccagc cggtggcccc cggcggcaag tacaaggaca accaggagct ttccttgggg
     1861 cgggcggagg ccgtgaagga ttacctgacg cagtcgggca tcgacccgcg ccgcctgtcg
     1921 gtgaagggca tgggagacac ccagccgatc gcctccaacg acacggcgga gggccgcgcc
     1981 aagaaccgcc gggtggagct gcaggtgacc ggcgagacca tcgccacggc ggcggacctc
     2041 atcgcgcagg ggaaggtctt ctacaactcg aaggacttca agggggcgct ggagcggttc
     2101 caggccgcca tcgaggccga gcccaaggac gccgaggcct accgccaggc ggggacctgc
     2161 tacctgcggt tgggcgacaa ggcggacgcc gtggcggcct tccgccaggc ctaccagttg
     2221 agccccgacc cccagttgaa ggcctggctg gaccagtacg ggaaggccaa ctaggccccg
     2281 gtccgctcgg cttcccgcag caggtcccgc aatttcatgt cccccatgga tccgatcctc
     2341 aggtccgccc ccgccgagtc caggtccaac cggcgggtca ggtcgttggg gaccgccacg
     2401 caaaagatcc ccgcggcctt ggcggccttg atcccgttgt gcgagtcctc gaaggccagc
     2461 gcctgggaag gcgcgatgcc cagggtctcc agcgtcagtt cgtaaagctc ggggtcgggt
     2521 ttgaccttgg ccacgtcgtc ccggcagcgg atcgaatgga agtaatccgc caaccccagc
     2581 cgggacaggt ggcccaccac ccaggcctgg gtggaactgg aagccactgc cacccgcagt
     2641 ttcatccggt aggcgtcgtc caggtactcc accacgccgg gccgggggac ctcccgcgcc
     2701 accagttcca ggttgggggc gcgttggcgg cgcaatacct cgtcccgggg cagcgcgcgg
     2761 cccagggacg actccaggtg ggcgtagggg tcgaagaggg aggcgaccgt gcccacccgg
     2821 agggcccagc ggtccagggg gaattccagc ccgtaatccg cgtaggtctc ccgccaggcg
     2881 ctgaaatcgg gcatctcggt gtccaggatc aagccgtcaa aatcgaagat cagggcttcg
     2941 a
//
LOCUS       DASTJJ010000558         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_566209, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000558 DASTJJ010000000
VERSION     DASTJJ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566209"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFR02_06865"
     CDS             complement(<1..747)
                     /locus_tag="VFR02_06865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9870196.1"
                     /translation="MKKTQKLNSRSAMMTEGDKRAPNRAMLRAVGFQDQDFSKPIVGI
                     ASAWSEITPCNYHLNELAERAKRGVQGAQGMAQTFGTPTVSDGIAMGHEGMKFSLVSR
                     EVIADAVETVSSAQRFDGLIAIGGCDKNMPGALIGIGRLNIPAVFIYGGTILPGKYEG
                     KDVDIVSVFEAVGQYSAGKIGKEALKAVECSACPGAGSCGGMYTANTMASAIEAMGMA
                     LPHDSCQPAVGEPKRLLCDQAGAAVVELIKK"
     gene            complement(791..1231)
                     /locus_tag="VFR02_06870"
     CDS             complement(791..1231)
                     /locus_tag="VFR02_06870"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010996531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9870197.1"
                     /translation="MPIQEGQPAPDFSLKATDGSTVALARFKGQKNVVLYFYPKDDTP
                     GCTKEACAFRDDLAPFAAKDAVILGVSVDDNTSHQAFSQKFNLNFPLLSDAGGAVSKA
                     YDAFNPEKGTSRRVTVVIGKDGAVKKFFPQVNVDGHSQEVLAAL"
     gene            complement(1325..>1586)
                     /locus_tag="VFR02_06875"
     CDS             complement(1325..>1586)
                     /locus_tag="VFR02_06875"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9870198.1"
                     /translation="AKEVEIRIWERGAGYTLASGSSSCAVAAAAVKTGRAGGDLTLKM
                     PGGQLSLTVSPDFDLTMRGPVEEICSGTLSADLLVFLEKNKA"
BASE COUNT          233 a          495 c          563 g          295 t
ORIGIN      
        1 cttcttgatg agctccacca cggccgcccc cgcctggtcg cacaggagcc gcttgggttc
       61 gcccacggcg ggctggcagg aatcgtgggg cagggccatg cccatggcct cgatggcgga
      121 ggccatggtg ttggccgtgt acatgccgcc gcaggaacca gcgcccgggc aggcgctgca
      181 ttcgacggcc ttaagggcct ccttgccgat cttgcccgcg ctgtactggc ccacggcctc
      241 gaagaccgac acgatgtcca cgtccttgcc ctcgtacttg ccgggcagga tggtgccgcc
      301 gtagatgaag accgccggga tgttgagccg gccgatgccg atcaaggcgc ccggcatgtt
      361 cttgtcgcag ccgccgatgg cgatgaggcc gtcgaaacgc tgggccgagg agacggtctc
      421 caccgcgtcg gcgatgacct cgcgggacac cagggagaac ttcatgccct cgtggcccat
      481 ggcgatgccg tcggacaccg tgggggtgcc gaaggtctgg gccatgccct gggcgccctg
      541 gacgccgcgc ttggcccgct cggccagctc gttcaagtgg tagttgcagg gcgtgatctc
      601 gctccaggcg gaggcgatgc ccacgatggg cttgctgaag tcctggtcct ggaaccccac
      661 ggcccgcagc atggcccggt tgggggcccg cttgtcgcct tcggtcatca tggcggaacg
      721 gctgttcagc ttctgggtct tcttcatggt ctgcctttct ggatggagtg ggacgccgct
      781 gcggggatcc ttagagggcg gccaagacct cttggctgtg gccgtccacg ttgacctggg
      841 ggaagaattt cttcacggcg ccgtccttgc cgatgaccac ggtcacccgg cggctggtgc
      901 ccttttcggg gttgaaggcg tcataagcct tcgacacggc gccccccgcg tccgacaaga
      961 gggggaagtt gaggttgaac ttttggctga aggcctggtg gctggtgttg tcgtccaccg
     1021 acacgcccag gatgacggcg tccttggcgg cgaagggggc caggtcgtcc cggaaggcgc
     1081 aggcttcctt ggtgcagccc ggcgtgtcgt ccttgggata gaagtaaagc accacgttct
     1141 tttggccctt gaaccgggcc agggcgacgg tgcttccgtc ggtggccttc aggctgaaat
     1201 cgggggccgg ctggccttct tggatgggca tgggacgctc ctggctcggg atgaaagaag
     1261 gggcatttta gtcgctttca gggggagaga caaggtccgg ccgggggccc ggccggaccc
     1321 ggttctaggc cttgttcttc tcgaggaaca cgagcaggtc ggccgagagg gtccccgagc
     1381 agatctcctc gaccgggccc cgcatggtca ggtcgaaatc gggggaaacc gtgaggctca
     1441 attgtccgcc cggcatcttg agggtcaggt ccccgcccgc gcggccggtc ttgacggcgg
     1501 cggcggccac cgcgcaggaa gaggagccgg aagccagggt gtagcccgcg cccctttccc
     1561 agatgcggat ctccacttcc ttggcg
//
LOCUS       DASTJJ010000559         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1668536, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000559 DASTJJ010000000
VERSION     DASTJJ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668536"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFR02_06880"
     CDS             <1..406
                     /locus_tag="VFR02_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HET9870199.1"
                     /translation="ASQQIMESKKNDADLKEALQKELRQFFRPEFLNRIDDIVIFHRL
                     GEDQLESIVAIQLAGLKARLADKKIALELSKGALAYLAEKGYDPVFGARPLKRLIQKE
                     LVDKLALKLLDGEIKDGQAVKVDVKGGELTIG"
     gene            494..700
                     /locus_tag="VFR02_06885"
     CDS             494..700
                     /locus_tag="VFR02_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870200.1"
                     /translation="MPAAKIKVIEQQIKRLDRDGLAAFRDWFRKYDAESWDRHVERDS
                     RNGKLGRLARHAVSEHKAGKTREL"
BASE COUNT          216 a          290 c          312 g          196 t
ORIGIN      
        1 cgcctcccag cagatcatgg aatcgaagaa gaacgacgcg gacctgaagg aggccctgca
       61 aaaggaactg cggcagttct tccggcccga attcctcaac cgcatcgacg acatcgtcat
      121 cttccaccgc ctgggcgagg accagctgga atcgatcgtg gccatccaac tggccggcct
      181 caaggcccgc ctggccgaca agaagatcgc gctggagttg tccaagggcg ccctggccta
      241 cctggccgag aagggctacg acccggtgtt cggggcgagg cccctgaagc ggctcatcca
      301 gaaggaactg gtggacaagc tggccttgaa actgctggac ggcgagatca aggacggcca
      361 ggccgtgaag gtggacgtca agggcgggga actgacgatc ggatgaagaa ggtggctccc
      421 gttgggggtc ctccctgctt tgtttgaccg accccctccc atgctataat ggcccatctt
      481 tttaggggga cctatgcctg ccgccaagat caaagtcatt gaacagcaaa tcaaacgtct
      541 tgaccgggac gggctcgcgg cttttcggga ttggttccgg aaatatgacg cggaatcctg
      601 ggaccgtcat gttgaacggg attcccgcaa cggaaaactc ggccggctcg cccgccatgc
      661 ggtttcagaa cataaggcgg gtaagacccg ggagctgtga aacactttac ttcctccggc
      721 ttcaggctcc gttaccgggg cctgccgaag gacgtccggg ccttggccga caaaagcttc
      781 tccctcctta agacgggccc gcatcaccct tcgttgcatt tcaagaaaat aggaaagtac
      841 cgttccgtcc gcattgggtt gaattacagg gccctggcga cggagacgtc cgatgggctc
      901 ctctggttct ggatcggaaa ccacgcggaa gatgacagga tgatccgttg atggggttgc
      961 tcgtgaatgt tgggcacatt cggcgggagg attcgatcct tcttccggtg gatc
//
LOCUS       DASTJJ010000560         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_935277, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000560 DASTJJ010000000
VERSION     DASTJJ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935277"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1068
                     /locus_tag="VFR02_06890"
     CDS             <1..>1068
                     /locus_tag="VFR02_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HET9870201.1"
                     /translation="FTDPLGHSLVSNSTPGTNITYVFDPAAFIDDDGQAYLYFGGGGP
                     GNARVIKLNSDMISTSGSAVSINAPRYFEAPFMNKINGTYYFSYSTDFSASPAASIDY
                     MTSSNPMTGFTHRGTVLNNPPNNCGNNDHAGIVNIGNNYYIAYHNRALALQNGLTCGG
                     NAVYQRSVNLDRLFVNSDGTLAVVAPTTAGVAALKNQDPYSTLRAVMMAKESGIQTEK
                     CSEGGMDVTATNSGDWTQVRNLDFGSGASSFTARVAGTVGGASLQIRLDSATGPLVGT
                     LSVPNTGGTQTWQNASCPISGATGLHDVFFVFAGGSGTLFNFQSYSFSGSTGPTATAT
                     RTATPVVSNTWRVNAGGPAYT"
BASE COUNT          195 a          369 c          325 g          179 t
ORIGIN      
        1 ccttcacgga cccgctgggc cattcgttgg tcagcaacag cacgccgggc accaacatca
       61 cctatgtgtt cgacccggcc gccttcattg acgacgatgg gcaggcctat ctttatttcg
      121 gcgggggcgg gcccggcaac gcccgggtca tcaagctgaa ctccgacatg atcagcacca
      181 gcggctccgc ggtctcgatc aacgcgccgc gttatttcga ggcccctttt atgaacaaga
      241 tcaacgggac ctattatttt tcctattcga cggatttttc ggcctcgccg gccgccagca
      301 tcgactacat gaccagcagc aaccccatga ccggcttcac ccaccgggga acggtcctca
      361 acaacccgcc gaacaactgc ggcaataacg accacgcggg gatcgtgaac atcgggaaca
      421 attactatat cgcctaccac aaccgggccc tggccctgca gaatgggttg acctgcggcg
      481 ggaacgcggt ctatcaacgc agcgtgaacc tggaccggct tttcgtgaac tccgacggca
      541 ccctggcggt cgtcgccccc accacggcgg gcgtggccgc gctcaagaac caggacccct
      601 attccaccct gcgggcggtc atgatggcca aggagtccgg gatccagacc gaaaagtgct
      661 ccgaaggggg catggacgtg accgcgacca acagcgggga ctggacccag gtgcgcaacc
      721 tggatttcgg gtccggggcc tcgtccttca ccgcccgcgt ggcggggacg gtcggcgggg
      781 ccagcctgca gatacgcctg gacagcgcca ccggccccct ggtggggacc ctgtccgtgc
      841 ccaacacggg cggcacccag acctggcaga acgcctcctg ccccatttcg ggggccacgg
      901 gcctgcacga cgtctttttc gtcttcgcgg gcggttcggg gaccctcttc aacttccagt
      961 cctattcctt cagcggttcg acgggcccga cggcgacggc gacgcgcacg gcgacgccgg
     1021 tggtgtcgaa cacgtggcgt gtgaacgccg ggggtccggc ctacacgg
//
LOCUS       DASTJJ010000561         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_689410, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000561 DASTJJ010000000
VERSION     DASTJJ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689410"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFR02_06895"
     CDS             <1..539
                     /locus_tag="VFR02_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9870202.1"
                     /translation="FDTLVKSFPSLIALGAARAGDERVRAVLAVNLYQECGEGDPSRS
                     HHAIYRKFLEGAGLQVGDAPDADFALRWRDALRDHLLSAPPGSVLGALAAGEFLAQPA
                     LSRLYPVLQAHFPDADQEYFTHHLELEAEHVEEITGVLKLQVEADGGTKDLAQGFEVG
                     LAAWEDYFSGLNSALAAR"
     gene            complement(536..1315)
                     /locus_tag="VFR02_06900"
     CDS             complement(536..1315)
                     /locus_tag="VFR02_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870203.1"
                     /translation="MSETIPVLDKPLEVPAYSAWDDGRLMAFTANEYMQKLYHRAKDI
                     GDDLYDSRTYEFFKLEPEAYRGNLITYMRRRAWSEWYFGTVAQARQITLLPEDQVNLK
                     LRLARQIQDEMRHYDVFAAQLKRYGAEPRISEFTLPPILVEMQKVQTEAQTAPEIAAS
                     NQFAGEIVLSSMTDLPTSVFKVLFDQELMAAVEDIEKDEPPHIANGKDLVLLGCGSAA
                     ERRGLARAQERYLGAMMQLHVTEIVKLGCRRVKPVPVFLAD"
     gene            complement(1370..2068)
                     /locus_tag="VFR02_06905"
     CDS             complement(1370..2068)
                     /locus_tag="VFR02_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870204.1"
                     /translation="MKNILWFLAVVMIFVPFAVLGLVWPHLPANLSGPGEVFIRQEAA
                     DPAGYRADLLAQSPALKAAGFTAYSFHRDLRDPKVTITTLKCSDLAQGLAYLRSAKFE
                     DALDKADPRIPQVWDGLDSKPRLYDKPLPKAPAGLVIARNEVRDYPFWLKCFFAEDGG
                     KHNHPGRHYQNSAYSIHHLPGKPAVAIVVHEASDVSKAPAFMRSGPLNGEMESTGVIG
                     LEVWYAVNLSSGTF"
     gene            complement(2090..3124)
                     /locus_tag="VFR02_06910"
     CDS             complement(2090..3124)
                     /locus_tag="VFR02_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9870205.1"
                     /translation="MKVLIIGGTGLISTAIVDQLVERGDDVTVVNRGVSEKRIPASVK
                     TIVGDRWDYPAFEKLMEGREYDAVIDMVAFAPENAESVLRAFTGRVKHILVCSTVCVY
                     GGPMTKLPAGDDEPHRPVGDYGKNKSKIESILLAADGKHGLRTTVLRPSFTTGEGATA
                     SGILFDDSLPSRLREGNPVIVMDDGRAAWAIAHVSDVARGFVNALLNPKAYGQAYHLT
                     SDEHTDWNGVFQTLAQAVNGKFDPVHIPTDWLYAQAPRRSVGVKFIYQYPSVFDNSKA
                     KRDLGFETRVPLAETFRRQVEWMDKKGLTKKVSEEPFEDLMIDAFRKGTKPLLPEGMD
                     FNPWGNGTTN"
     gene            complement(3292..>3644)
                     /locus_tag="VFR02_06915"
     CDS             complement(3292..>3644)
                     /locus_tag="VFR02_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9870206.1"
                     /translation="RPDAVVVHLGTNDFSTEPHPDPEDFQRSSVALLAHLRAQYPQAS
                     LFWFAATGWPDYYPAVERAVAARHAAGDAKVYCVGYPPVPADQMGCDFHPNVKVHQKL
                     ATLLTRSLRQKLGW"
BASE COUNT          593 a         1106 c         1261 g          684 t
ORIGIN      
        1 ccttcgacac cctggtgaaa agcttcccct cgctcatcgc gttgggcgcc gcccgcgcgg
       61 gggacgagcg ggtgcgcgcc gtgttggcgg tgaacctcta tcaagaatgc ggcgaagggg
      121 acccttcccg gtcccaccac gccatctacc gaaaattcct cgagggcgcg gggctccagg
      181 tcggcgacgc cccggacgcg gatttcgccc tgcgttggcg ggacgccttg cgggaccatc
      241 tgctttccgc gccaccgggt tcggtgctgg gcgccctggc cgccggggag ttcctggccc
      301 agcccgccct cagccggctc tatccggtcc ttcaggccca tttcccggac gccgaccagg
      361 aatatttcac ccatcacttg gagttggagg ccgagcacgt ggaggagatc accggcgtgc
      421 tgaagctgca ggtggaggcc gacggcggca cgaaggacct ggcccaaggg ttcgaggtcg
      481 ggctcgccgc ctgggaagat tacttcagcg gcctgaattc ggccctggcg gcgcgctagt
      541 ccgctaaaaa gaccggcacg ggtttgaccc gccggcagcc caacttgacg atctcggtga
      601 catgcagctg catcatggcc cccaggtatc tttcctgggc ccgggccagg ccccgccgct
      661 cggcggcgct gccgcagccc aagagcacca agtccttccc gttggcgatg tggggcggct
      721 cgtccttctc gatgtcctcc acggccgcca tcagttcctg gtcgaagagc accttgaaga
      781 cgctggtggg caggtcggtc atggaggaca gcacgatctc gcccgcgaac tggttggagg
      841 cggcgatctc gggggcggtc tgggcctcgg tctgcacctt ctgcatctcc accaaaatgg
      901 gcggcagggt gaattccgaa atgcggggtt cggcgccgta gcgcttgagc tgggcggcga
      961 agacgtcgta gtggcgcatc tcgtcctgga tctggcgggc caggcgcagc ttcaggttca
     1021 cctggtcctc gggcaggagg gtgatctggc gggcctgggc cacggtgccg aaataccatt
     1081 cgctccaggc ccggcggcgc atgtaggtga tgaggttccc ccggtaggcc tccggctcca
     1141 gcttgaagaa ctcgtaggtg cgggaatcgt aaaggtcgtc gccgatgtcc ttggcccggt
     1201 ggtagagctt ctgcatgtac tcgttggccg tgaaggccat gaggcggccg tcgtcccagg
     1261 ccgaataggc ggggacctcc aggggtttgt ccaggaccgg gatcgtttcg gacatcgaag
     1321 cctcctttat ctgcaaactc agcacttcct gatcagacgt cccgcggggt caaaaggtcc
     1381 cggaggacag gttcaccgcg taccagacct ccaggccgat gacgccggtg gattccatct
     1441 cgccgttcag ggggccggaa cgcatgaagg ccggggcctt ggagacgtcg gaagcctcgt
     1501 gcaccacgat ggccacggcg ggtttgccgg gcaggtggtg gatgctgtag gcgctgttct
     1561 ggtagtggcg gcccgggtgg ttgtgcttgc cgccgtcctc ggcgaagaag cacttgagcc
     1621 agaaggggta gtcgcgcacc tcgttgcggg cgatgaccag gcccgccggg gccttgggca
     1681 ggggcttgtc gtagaggcgg ggtttgctgt ccaggccgtc ccagacctgg gggatgcggg
     1741 ggtcggcctt gtccagcgcg tcctcgaact tggccgagcg caggtaggcc aggccctggg
     1801 cgagatcgga acatttcagc gtagtgatgg tgaccttggg gtccctcagg tcgcggtgga
     1861 agctgtaggc ggtgaagccc gcggccttga gtgcggggct ttgggccagt aggtcggccc
     1921 ggtagcctgc cgggtcagcc gcctcttgac ggatgaagac ctcgccgggc cccgagaggt
     1981 tcgcgggtag gtggggccag accagcccca agaccgcgaa gggaacgaag atcatcacca
     2041 cggccaggaa ccaaaggatg tttttcatgc cggagccgcc ttccttttat taattcgtcg
     2101 tgccgttgcc ccaggggttg aagtccatcc cctcaggcag gagcggtttc gtccccttcc
     2161 ggaaagcgtc gatcatcagg tcctcgaagg gctcctcgga gacctttttc gtcagccctt
     2221 ttttgtccat ccattccacc tggcggcgga aggtctccgc cagggggacg cgggtctcga
     2281 aacccaggtc ccgcttggct ttcgagttgt cgaagacgga gggatattgg tagatgaact
     2341 tcacgcccac gctgcggcgc ggggcctggg catagagcca atccgtgggg atatggaccg
     2401 gatcgaactt gccgttcacg gcctgggcga gggtttggaa gaccccgttc cagtcggtgt
     2461 gctcgtcgct ggtcaaatga taggcctggc cataggcctt ggggttgagc agggcgttga
     2521 cgaagccgcg ggccacgtcg gacacgtggg cgatggccca ggcggcccgg ccgtcgtcca
     2581 tcacgatgac ggggtttccc tccctcaggc ggctgggcag gctgtcgtcg aagaggatgc
     2641 ctgaggccgt ggcgccctca ccggtggtga aggaaggccg cagcacggtc gtccggaggc
     2701 cgtgtttgcc gtcggccgcg agcaggatgg actcgatctt ggacttgttc ttcccgtaat
     2761 cgcccacggg ccggtggggt tcgtcgtcgc cggcgggaag tttggtcatg ggcccgccgt
     2821 agacgcagac cgtggaacag accaggatgt gcttcacccg ccccgtgaag gcccgcagga
     2881 cggactcggc gttctcgggc gcgaaggcca ccatgtcgat gaccgcgtcg tattcccggc
     2941 cctccatcag cttctcgaag gcggggtagt cccagcggtc gcccacgatg gtcttgaccg
     3001 aggcggggat gcgcttttcg ctcacccccc ggttgaccac cgtgacatcg tcgccgcgct
     3061 cgaccagttg gtccacgatg gcggtggaga tgagccccgt cccgccgatg atgagcactt
     3121 tcataaaaac ctttcggaca gaagatccgg cttgatcact tttaccacga agtccactca
     3181 gttcacgaag taaagacaag atttatgaag gacggggaaa accagaagtc ttattttcgc
     3241 cttacttcgt gaccttcgtg cacttcgtgg tggaatagat gtttggggtt attaccaacc
     3301 cagcttttga cgcagggacc gggtcagcag ggtcgccagc ttttggtgca ccttcacgtt
     3361 ggggtggaag tcgcagccca tctggtcggc gggcacgggc gggtaaccca cgcagtagac
     3421 cttggcgtcg cccgcggcgt ggcgggccgc cacggcccgt tccaccgccg ggtaatagtc
     3481 cggccagccg gtggcggcga accagaagag ggacgcctgg ggatattggg cccgcaggtg
     3541 ggccaagagg gccaccgagc tgcgctggaa atcctccggg tcgggatggg gttcggtgga
     3601 aaagtcgttg gtgcccaagt gcaccaccac ggcgtcgggc cgcc
//
LOCUS       DASTJJ010000562         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_935517, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000562 DASTJJ010000000
VERSION     DASTJJ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935517"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFR02_06920"
     CDS             <1..806
                     /locus_tag="VFR02_06920"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9870207.1"
                     /translation="KRVLVALVAVPLLLAATFCPSPWVFKALVVAALLAALQEFFRMA
                     PDQRVRPQAWVSHLCLLALLAPLFFPGLPFFSLREAVLLALALPCLAFLFSARPPQEM
                     LPAVPFASFAALYFGLLGGYFLSLRDLPRGPWLLLFVYVATWAYDTGGYFAGRFLGRH
                     KMTPQTSPQKTWEGALGGLVLALGALFLLWACCPAYREIFRAGDLAALGVLLSLFGQL
                     GDLVESMVKRSLAAKDSGSFFPGHGGVFDRIDSLLFTAPVLFYYLTLRP"
     gene            873..>2037
                     /locus_tag="VFR02_06925"
     CDS             873..>2037
                     /locus_tag="VFR02_06925"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET9870208.1"
                     /translation="MALEDLIKKAQSTFHHQFGEVGRHLTLVAAPSRVTLLGGEYTEA
                     VDGFSLVAAGNHWVVVAAQRRNDRQAAIHSLNYDEKVKVNLATLKFDRADGWANFPKG
                     VLYFYERTGRKSEGIQMTIVSDIPENAGLGASAALSAATGVACNILGTQPLDDPTLVK
                     LCQRVESQFIGGRGDYFSPFASKMGKKDHLVVFDARTFKPEYLPFDSKAYKLVVVDSG
                     VKRKEREDEFKKRLELFGQLLAEIRKHVPKVISLRDVGGEAYEDARKKIDIILRKRLD
                     HVVYENQRVKRAKDLMARGDYAGLGGLLNESHASLADKLKVTCGEVDILWGITQALPG
                     CLGARMAGIGWGGHLVCLVEAPQVQAFADQVRGEYKKRVNINAQVYVYEPQDGA"
BASE COUNT          291 a          684 c          687 g          375 t
ORIGIN      
        1 tgaagcgggt gttggtggcc ctggtggccg tgcccctgct cttggccgcc accttctgcc
       61 ccagcccctg ggtcttcaag gccctggtgg tggcggcgct cctggccgcc ctccaggaat
      121 ttttcaggat ggccccggac cagcgtgtcc ggccgcaggc ctgggtgtcc cacctttgcc
      181 tgctggccct gctggcgccc ctctttttcc ccggcctgcc cttcttcagc ctgcgggagg
      241 ccgtgctcct ggccctggcg ctgccctgcc tggccttcct cttctcggcg cggccgcccc
      301 aggagatgct gcccgcggtg cccttcgcca gcttcgcggc gctttatttc ggcctgctgg
      361 gcggttattt cctctcgctg cgcgacctgc cccgcggccc ttggctgttg ttgttcgtgt
      421 atgtggccac ctgggcctat gacacgggcg gttatttcgc cggccgcttc ctgggccgcc
      481 acaagatgac gccccagacc tcgccgcaga agacctggga aggcgccttg ggcgggctgg
      541 tcctggcgct gggggccctg ttcctgctct gggcctgttg cccggcctat cgggagatct
      601 tccgggcggg ggacctggcg gcgctggggg tcctgctgtc gctgttcggc cagttgggcg
      661 acctggtgga atccatggtc aagcgcagcc tggccgccaa ggattcgggg tcctttttcc
      721 cgggccacgg cggcgtgttc gaccgcatcg acagcctgct cttcaccgcg cccgtgctgt
      781 tctattacct gacgctgagg ccttgaaggc ccgccgctcg gcgtcttttt cggccgcggg
      841 ccggccttaa atcgcagtcg caaggagacg ttatggccct cgaggacctg atcaagaaag
      901 cccagagcac cttccaccac cagttcggcg aggtgggccg gcatttgacc ctggtggccg
      961 cgcccagccg cgtcaccctg ttggggggcg agtacaccga ggcggtggac ggcttttccc
     1021 tggtggcggc ggggaaccac tgggtggtgg tggcggccca acggcgcaac gaccggcagg
     1081 cggccatcca ttcgctcaac tacgacgaga aggtcaaggt gaacctggcc accctgaagt
     1141 tcgaccgggc ggacggctgg gccaacttcc ccaagggcgt gctctatttc tacgagcgca
     1201 cgggccgcaa gtccgagggc atccagatga ccatcgtgag cgacatcccg gagaacgcgg
     1261 gcttgggcgc ctcggcggcg ctgtcggccg ccacgggcgt ggcctgcaac atcctgggca
     1321 cccagcccct ggacgacccc accctggtca agctctgcca gcgggtggaa agccagttca
     1381 tcggcgggcg gggcgattat ttttccccct tcgcctccaa gatgggcaag aaggaccacc
     1441 tggtggtctt cgacgcgcgc accttcaagc ccgagtacct gcccttcgat tccaaggcct
     1501 acaagctggt ggtggtggac agcggggtca agcgcaagga gcgcgaggac gagttcaaga
     1561 agcgcttgga gctcttcggc cagctgctcg ccgagatccg caagcacgtg cccaaggtca
     1621 tttcgctccg ggacgtgggc ggggaggcct atgaggacgc ccgtaagaag atcgacatca
     1681 tcctgcgcaa gcgcctggac cacgtggtct acgagaacca gcgcgtgaag cgcgccaagg
     1741 acctgatggc ccggggcgac tacgcggggc tcggcgggct cctgaacgaa tcccacgcct
     1801 cgctggccga taagttgaag gtcacctgcg gcgaggtcga catcctgtgg ggcatcaccc
     1861 aggccctgcc gggctgcctg ggcgcccgga tggccggcat cggctggggc gggcatttgg
     1921 tctgcctggt ggaggccccc caggtgcagg ccttcgccga ccaggtccgg ggggaataca
     1981 aaaagcgggt caacatcaac gcccaggtct atgtctacga accccaggat ggggccc
//
LOCUS       DASTJJ010000563         1141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1423619, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000563 DASTJJ010000000
VERSION     DASTJJ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423619"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFR02_06930"
     CDS             <1..882
                     /locus_tag="VFR02_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HET9870209.1"
                     /translation="DNHLPAPLQTFASLYGSWLEALRSDRHAESTHGKAWQLVGLLLL
                     DLLCLVVIVITTASTVKRWLPQWEALFHLPPAWGKAALIGVGMTLAVPFAAGIFRCAK
                     NLGGLLAAAAIPPVAEGSVDLGLAPRKLLTVTLQLGIVFAVGVPLVALTQPFLPFGSV
                     TFILLGLLAGLGILFWKSAINLQDHVHAGAQMVTDALTHHSQERSEAFYEQMNAFVPG
                     LGSPTPVTVGESSQASGRTLLELNLRNRTGVGVIAIMRGQERILMPPPRETLQAGDIL
                     ILVGTQQGLQAAKALLT"
BASE COUNT          174 a          408 c          339 g          220 t
ORIGIN      
        1 gacaaccacc tgcccgcgcc gctccagacc ttcgcctccc tctacggctc ctggctggag
       61 gccttgcggt ccgaccggca cgccgaatcc acccacggca aggcctggca attggtgggc
      121 ctgctgcttt tggacctttt gtgcctggtc gtcatcgtca tcaccacggc ctccacggtg
      181 aaacgctggc tcccccagtg ggaagccctg ttccacctgc cccccgcctg ggggaaagcc
      241 gcgttgatcg gggtgggcat gacgctggcc gtgcccttcg cggcgggcat cttccgctgc
      301 gccaagaacc tggggggcct gctggccgcg gcggccatcc cgccggtggc ggaggggtcg
      361 gtcgacctgg ggctggcgcc ccgcaagctc ttgaccgtga ccctgcagtt gggcatcgtg
      421 ttcgcggtgg gggtgccgct ggtggccctg acccagccct ttttgccctt cggctcggtc
      481 accttcatcc tcttggggct gctggccgga ctgggcatcc tcttctggaa gagcgccatc
      541 aaccttcagg accacgtgca tgccggcgcc cagatggtca ccgacgccct gacccaccat
      601 tcgcaggaac gcagcgaggc cttctatgag cagatgaacg ccttcgtgcc cggcctgggc
      661 agccccaccc ccgtcacggt gggcgaaagc agccaggctt ccggccgcac cctgttggaa
      721 ttgaacctgc gcaaccgcac gggggtgggc gtcatcgcca tcatgcgggg ccaggaacgc
      781 atcctgatgc cgccaccccg ggagaccctc caggcgggcg acatcctgat cctggtgggc
      841 acccaacagg gcctccaggc cgccaaggcg ctgttgacct gaccccaacg cccactcttt
      901 gtcacgattc cacccggaca agccaattat tttactttta gccccgactc ccatccttcc
      961 atcatgggga aaggctttga aagtccccct ttgacctggc acataatttg ccccttcatg
     1021 gccgatgcat gccggaccca tggagggatg cccgatgagg aagcttttta ccgtttggtg
     1081 cctcttgccc gccgcggtgt tgttcgtcgg atgccagaaa tccatcgtga tcgcccccac
     1141 c
//
LOCUS       DASTJJ010000564         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1179419, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000564 DASTJJ010000000
VERSION     DASTJJ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179419"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFR02_06935"
     CDS             complement(<1..318)
                     /locus_tag="VFR02_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9870210.1"
                     /translation="MAAVLALGPWVQGCTLLFVAKSEVRYIQDPAPTVVPAASLSRQM
                     SGRVVLRYKVRPGDTLRSIALLYYGDPSRAARVASDNHLRAGARLREGRTLRIVDPLD
                     FPGP"
     gene            complement(356..874)
                     /locus_tag="VFR02_06940"
     CDS             complement(356..874)
                     /locus_tag="VFR02_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870211.1"
                     /translation="MRRFFFPFLFFFAWAPLAGAQVDPTDPIQTHWTTLGGMEYLRDG
                     RSLDGAELEKIIDSAGDRQASALLQRSGSDETLGFFGLGGSVALSVLSLFFPNSHIHV
                     VGLDISTPYLPLAVPGAVLGVAGGLLELEANTAKYAAVQRYTRLMRRPDPLTWGFSPD
                     GQGLTLRVACAF"
     gene            complement(963..1496)
                     /locus_tag="VFR02_06945"
     CDS             complement(963..1496)
                     /locus_tag="VFR02_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870212.1"
                     /translation="MNWKIPAILLTVGLGTVWARTPTPSTASSPWETVYNKTCGYWLR
                     YPKGVKWERVGDCSFKLTWPETAIIAGMTLTLEVTPVHNGEADVPVDSEDPPTGSLVA
                     GGLKFEKTVEDDRGAGHALVTVTYVALGKTHQYYWVGVLNAVEPSILGTPTPDWDPGK
                     MAEKMFDGVLLGFRPLE"
     gene            complement(1493..>2795)
                     /gene="glpK"
                     /locus_tag="VFR02_06950"
     CDS             complement(1493..>2795)
                     /gene="glpK"
                     /locus_tag="VFR02_06950"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET9870213.1"
                     /translation="VRPSAVAAVGITNQRETTLLWDRRTGKPIHRAIVWQCRRTAHRC
                     DELKARHGRLFRERTGLLIDAYFSGTKVEWLLRHVPGAARRAARGELAFGTIDSWLLW
                     NLTGGKVHATDHTNASRTLLYDIRAKRWDPALLKTLHVPASLLPKVLPSSGLFGRTSA
                     KSYLGEGVPVTGIAGDQQAALFGQGCHEPGSLKNTYGTGCFLLLNLGKKFLLSKNQLL
                     TTLGCDEKGRPAYVLEGAVFIGGAAVQWVRDGLGLIKTSPESEALARSVPDTGGVYLV
                     PAFVGLGAPYWDQHARGALVGLTRGSGRAQVVRAAVESMAYQTRDLADAMAKDSHVKL
                     RELRVDGGACKNDLLMQFQADILNVRINRPRVVETTAMGAAYLAGLAVGFWKDAAQIQ
                     KIRRVDKVFKPKMKPGERAALWAGWKKAVGQVLTVPGRGKE"
BASE COUNT          475 a          972 c          898 g          450 t
ORIGIN      
        1 ggggcccggg aaatccaacg gatcgacgat ccggagcgtc cgtccctccc tcaaccgggc
       61 ccccgcgcgc aagtggttgt ccgacgccac ccgcgccgcc cgggacggat ccccgtaata
      121 gaggagggcg atcgaccgga gggtgtcccc cggccggacc ttgtatcgca ggaccacgcg
      181 cccgctcatc tgccgggata aggacgccgc agggaccacc gtgggcgcgg ggtcctgaat
      241 gtaccggacc tcgcttttcg ccacgaagag cagcgtgcag ccctggaccc aaggccccaa
      301 cgccagtacc gccgccaaca ggatgcccgg aggaaccggg cccctccggg gcgccttaga
      361 aggcacaggc cacccgcagg gtgagtccct gcccatcggg ggaaaagccc caggtcaatg
      421 gatcgggccg cctcatcagc cgggtataac gctggacggc ggcgtacttg gccgtattgg
      481 cctccagttc taaaagcccg ccggccacgc ccaaaacggc ccccggcacc gccaggggca
      541 gatagggggt ggagatgtcc aatccaacga catgaatatg gctgttggga aagaacaggg
      601 aaaggacgga cagggccacc gatccaccca gcccgaaaaa gcccaacgtt tcgtccgacc
      661 ccgatctttg gaggagggcg gaggcctgtc gatcaccggc cgaatcgatg atcttttcca
      721 attcggcccc gtccagcgac ctgccgtccc gcaggtattc catcccgccc aaggtcgtcc
      781 aatgggtctg gatggggtcc gtggggtcga cctgcgcccc ggccaagggc gcccaggcga
      841 agaagaacag gaaggggaaa aagaacctcc gcacgggcgc tctccttttt gtgggtccat
      901 ccatcagttg aagcccccgg gtccggccca tggtcttttg tccggcatgg gtcgttcccg
      961 gcctattcca acggccggaa gcccaagagc accccatcga acatcttctc ggccatcttg
     1021 cccgggtccc agtccggggt gggcgtcccc aggatcgagg gttcgaccgc gttcaggacc
     1081 cccacccaat aatattggtg ggtcttgccg agggccacgt aggtcaccgt gaccagggca
     1141 tggcccgccc cccggtcatc ctcgacggtc ttctcgaact tcagcccgcc cgccaccaag
     1201 gaacccgtcg gggggtcttc gctgtccacc gggacatccg cttccccgtt gtggacgggc
     1261 gtgacctcca gcgtcaaggt catcccggcg atgatggcgg tctcgggcca ggtgagctta
     1321 aaggagcaat cgccgacgcg ctcccatttc acccccttgg gatagcgcag ccaataaccg
     1381 caggtcttgt tgtagacagt ttcccagggg gacgacgccg tcgatggggt gggcgtccgg
     1441 gcccagacgg tccccagccc caccgtcagc aggatcgccg gtatcttcca attcattctt
     1501 tccctcttcc cggcacggtc agcacctgtc ccaccgcctt cttccaaccc gcccacaagg
     1561 ccgcccgttc ccccggcttc atcttgggct tgaagacctt gtccacccgg cggatcttct
     1621 ggatctgggc ggcgtccttc caaaagccca cggccagccc ggccagatag gccgcgccca
     1681 tggcggtggt ctccaccacc cggggacggt tgatgcgcac gttcaagatg tccgcctgga
     1741 actgcatcaa gaggtcgttc ttgcaggccc cgccgtccac ccgcagttcc cttaatttca
     1801 catgggaatc cttggccatg gcgtccgcca ggtcccgggt ctggtaggcc atggattcca
     1861 ccgcggcccg gaccacctgg gcccggccgc tgccccgggt gagcccgacc aaagcgccgc
     1921 gcgcgtgctg gtcccaatag ggcgcgccca gccccacgaa ggccggcacc aggtagaccc
     1981 cgccggtgtc gggcaccgag cgggccaggg cctcggactc cggcgaggtc ttgatgagcc
     2041 cgagcccatc ccgcacccat tggacggccg ccccgccgat gaagaccgcc ccctccagca
     2101 cgtaggccgg cctccccttt tcgtcgcagc ccaaggtggt caagagctgg ttcttggaca
     2161 ggaggaattt tttccccagg ttgagcagca ggaaacaacc cgtgccgtag gtgttcttca
     2221 agctgccggg ctcgtggcaa ccctggccga agagcgcggc ctgctggtcg cccgcgatgc
     2281 cggtgacggg cacgccctcc cccaggtagc tcttggccga agtgcgcccg aaaagcccgg
     2341 aggacgggag gaccttcggg agcagggagg ccgggacgtg gagcgtcttg aggagcgccg
     2401 ggtcccaccg cttggcgcgg atgtcgtaaa gcagggtgcg ggaggcgttg gtgtggtcgg
     2461 tggcgtggac cttgccgccg gtcaggttcc aaagcagcca gctgtcgatg gtgccgaagg
     2521 ccagttcccc ccgggcggcc cggcgggccg cgcccggcac gtgccgcaac agccattcca
     2581 ccttggtgcc cgagaaataa gcgtcgatga gcaggccggt cctttcgcgg aacaggcggc
     2641 cgtggcgcgc cttcaactca tcgcagcggt gcgcggtgcg gcggcattgc cacacgatgg
     2701 cccggtggat gggcttgccg gtcctccggt cccagagcag ggtggtttcc cgttggttgg
     2761 tgatgccgac cgccgccacg gcggaaggcc ttacg
//
LOCUS       DASTJJ010000565         1934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1301752, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000565 DASTJJ010000000
VERSION     DASTJJ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301752"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..540
                     /locus_tag="VFR02_06955"
     CDS             220..540
                     /locus_tag="VFR02_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870214.1"
                     /translation="MRSLLPLRLACVLGFLKAASNMAVIYSPTIWQVSNAYPAYFVAA
                     STLSVVALGGLWALRRWGLWLYIAYALFAQAVPWMLHRWDSSILVLPAATLAVAAFYY
                     RRLQ"
     gene            537..1559
                     /locus_tag="VFR02_06960"
     CDS             537..1559
                     /locus_tag="VFR02_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HET9870215.1"
                     /translation="MKASGRLAPPAPFSLGLTLLCGQCFRWEGPDAQGVFEGVAGGAF
                     WRLRQDGELFWECSASEVRGGEPGVWLVRYLGLDEDLSGWVGEWENHSVMEKPLAALR
                     GLRLMRQEPWECLVSYMFAQGLSVKVIRQALRKFCEAVGSPLEGVPGKYDFPGADRLA
                     GLSPDVLRPFTNNYRARADRIIRAARSVRAGVISLDHLATLPCDQAREALMGLDGIGP
                     KIADCVLLFSLDQPQAFPLDRWVLRAMRRHFRSVKLLGNTGEAPTPSQYPKIVAKARR
                     GLGVRCGLVSEYLFLYLRLLEDAPLRRELAPHLSAPLPPLPAPGRPRRKPGTPSPGRE
                     NFSRIR"
     gene            1638..>1934
                     /locus_tag="VFR02_06965"
     CDS             1638..>1934
                     /locus_tag="VFR02_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870216.1"
                     /translation="MKPSLLIVDDEESVRESFRLALQDDYDLTFGEDSKSTLAILKSR
                     VFDLVLLDIMLPDGSGMDLLRQVKRRGESMDVIMVTALQSIDTALEAMKWGAYDY"
BASE COUNT          290 a          634 c          638 g          372 t
ORIGIN      
        1 ggtgggttgc agaaagtctc ggaagtggtg caaccggatg accttcaagt ggaaccgggg
       61 tatccacatt cccatggcga cgatagaggc ggggatgaaa aacaccggtg attttgtggc
      121 ctcggccggc cgatcgaact gaaagggcgg ccatcgaggg gacggccgcc ggaactgaac
      181 cccgccccaa aggcggtcgc ctttggtatg attcccccca tgagatcgct ccttcccctg
      241 cgcctggcct gcgtgctggg cttcttgaag gccgcttcca acatggcggt catctattcc
      301 ccgaccatct ggcaggtgag caacgcctat ccggcttatt tcgtcgcggc cagcaccctg
      361 tcggtggtgg ccctgggggg cttgtgggcg cttcggcgtt ggggcctgtg gctttacatc
      421 gcctacgccc tcttcgccca ggccgtaccc tggatgctcc accggtggga cagctcgatc
      481 ctggtcctgc cggccgccac cctggcggtg gccgccttct actaccggcg gctgcaatga
      541 aggcctccgg gcgtttggcg ccgcccgcgc ccttttccct ggggctgacg ctgctttgcg
      601 ggcaatgttt ccgttgggaa ggccccgacg cgcagggcgt cttcgagggc gtggccgggg
      661 gcgccttctg gcggctgcgc caggacgggg aactgttctg ggaatgttcg gcctctgaag
      721 tccgcggggg cgagccgggg gtttggctcg tccgttatct ggggttggac gaggacctat
      781 ccggttgggt gggggagtgg gaaaaccact ccgtgatgga aaagcccctg gcggcgttgc
      841 ggggccttcg cctgatgcgg caggaacctt gggaatgcct ggtgtcctac atgttcgccc
      901 aggggctttc ggtcaaggtc attcgccagg ccctgcgcaa gttctgcgag gcggtgggtt
      961 cccccctgga aggtgtcccg ggaaaatacg atttccccgg ggccgaccgc ctggccggcc
     1021 tttcccccga cgtcctgcgg cccttcacca acaactaccg ggcccgggcc gaccgcatca
     1081 tccgcgcggc gaggtccgtg cgggcggggg tgatctcgct ggaccacctg gccacgctcc
     1141 cctgcgacca ggcccgggag gccctgatgg gcctggacgg gatcggcccc aagatcgcgg
     1201 actgcgtcct gctcttttcc ctcgaccaac cccaggcctt cccgttggac cgctgggtgc
     1261 tccgggccat gcggcgccat ttccggtcgg tgaagctcct gggcaacacc ggggaagcgc
     1321 ccacgccttc ccaatatccc aagatcgtgg ccaaggcccg ccggggtttg ggggtccggt
     1381 gcgggttggt gagcgagtac ctgttcctct acctgcggtt gctggaggac gcccccttgc
     1441 gccgcgagtt ggccccccac ctgtcggccc ccttgccgcc cctgccggcg cccggccggc
     1501 cccgccgcaa gccgggaacc ccttccccgg gccgtgagaa tttttcaaga attaggtgac
     1561 atccgcccgg ccttgcctgt attttataaa tgttggataa actgtgcagg gttgtagcgc
     1621 ccgcctgggg gtcacccatg aaaccatcat tattgatcgt ggacgacgag gaaagcgtcc
     1681 gggaatcctt ccgcctggcc ctgcaggacg attacgacct caccttcggc gaggattcca
     1741 aaagcacgct ggccatcctc aagagccggg tcttcgacct ggtgctcctc gatatcatgc
     1801 tgcccgacgg ctcgggcatg gacctgctgc gccaggtcaa acgccgcggc gagagcatgg
     1861 acgtcatcat ggtgacggcc ctgcagagca tcgacacggc cctggaagcc atgaaatggg
     1921 gcgcctacga ctac
//
LOCUS       DASTJJ010000566         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_445881, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000566 DASTJJ010000000
VERSION     DASTJJ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445881"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2011
                     /locus_tag="VFR02_06970"
     CDS             <1..>2011
                     /locus_tag="VFR02_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HET9870217.1"
                     /translation="PGTGAAWYREYTKYYGGTSTGRTGFNSGAIAMDALGNYGAWESS
                     VDSWQSTILTGNYPDWLKQMLFNELYYYFTGGTMWEAGKVGDASFDTKPNMFSSLESY
                     IYDFYGTSDVRFYGSWPLALLWPDIDKQEVKQFCDSVVTGTAMGGLGPLSAATCAHDI
                     GDDNGVFTEWNAYVYRDSTTWKDLNSKLVLMVYRAWELTGKTDSSFLNYCWPAVQAAM
                     TKVHGQCDASGLPISSGIDQTYDDLGLTGDTAYCGSLFLAACEAAQAMATQEGNSALA
                     ASYGNWLSTGQASFESLLWDNTGGYYHIDTGSSAPNRIMSDQLCGEWYAKALGLPGIV
                     SDAHADSAWQKVHDDNWLKFDGGAHGVVNVMTSAGAIDTSYPQSQEAWVGVGWSVAAG
                     LVQQGLFTQATDIGYSLYNSIWNTGQLWFRTPEAWQTGLTSVRAPYYMRANAIWALKH
                     AYDIAPPPCGLLTCTATPTLSPTATQTPTATVNPCAFVYQTVDCGGPATVIAGVSWAA
                     DQAYTAGGFGYVTAGTAASTGSAIAGSGAQQPLYQTERYAATLEYKFNVPNGLYQVTL
                     KNAEQYYTTTGNRVFSVAINGVTVITNMDLVAQVGAFTADDHTFAVSVTGGLIDIVGT
                     ASKDNAEFTAIEVVSEAPCSPTPTPTPTRTATRTPTPSPTVSPTVS"
BASE COUNT          373 a          698 c          633 g          307 t
ORIGIN      
        1 acccggcacc ggcgcggcct ggtaccggga atacaccaag tattacggcg ggacctccac
       61 gggccgcacg gggttcaatt cgggggccat cgcgatggac gccctcggca attacggcgc
      121 ctgggaatcc tccgtcgaca gctggcagtc gaccatcctg acgggcaact atccggactg
      181 gctcaagcag atgctcttca acgagcttta ctattacttc accggcggca ccatgtggga
      241 ggcgggcaag gtgggggacg cctccttcga caccaagccc aacatgttca gcagcctgga
      301 gagctatatt tatgattttt acggcacgtc ggacgtccgt ttctacggct cctggcccct
      361 ggcgctgctc tggcccgaca tcgacaaaca ggaggtcaag cagttctgcg acagcgtggt
      421 gacgggcacg gccatgggag gcctggggcc cctctcggcc gccacctgcg cccacgacat
      481 cggggatgac aacggggtct tcaccgaatg gaacgcctat gtgtaccggg attccaccac
      541 ctggaaggac ctgaactcca agctggtgct gatggtctac cgggcctggg agttgacggg
      601 caagaccgac agcagcttcc tgaactattg ctggcccgcc gtacaggcgg cgatgaccaa
      661 ggtgcacggc caatgcgacg cctcggggct gccgatctcc agcggcatcg accagaccta
      721 cgacgacctg gggctgacgg gggacacggc ctattgcggg agcctgttcc tggcggcctg
      781 cgaggcggcc caggccatgg ccacgcagga agggaacagc gccttggccg ccagctacgg
      841 caactggctc tccacgggcc aggccagttt cgagtcgctc ctctgggaca acacgggggg
      901 ctattaccac atcgacacgg gcagctcggc ccccaaccgc atcatgagcg accagctgtg
      961 cggggaatgg tacgccaagg ccttggggtt gcccggcatc gtgtcggacg cgcacgccga
     1021 cagcgcttgg cagaaggtcc acgacgacaa ttggctcaag ttcgacggcg gggcccacgg
     1081 ggtggtcaat gtcatgacct ccgccggggc catcgacacc agctatcccc aaagccagga
     1141 ggcctgggtg ggggtggggt ggagcgtggc ggccggcttg gtgcaacagg ggctgttcac
     1201 ccaggccacc gacatcggct acagcctcta caacagcatc tggaacacgg gccaactttg
     1261 gttccgtacg ccggaggcct ggcaaacggg gctgacctcg gtccgggcgc cctattacat
     1321 gcgggccaac gccatttggg cgctcaagca cgcctatgac atcgccccgc ccccctgcgg
     1381 cctcctgacc tgcaccgcca cgcccaccct cagccccacg gcgacccaga cccccacggc
     1441 caccgtcaac ccctgcgcct tcgtctacca gacggtcgat tgcggcgggc cggccacggt
     1501 catcgccggc gtttcgtggg cggcggacca ggcctatacc gccggagggt tcggttacgt
     1561 gaccgcgggg accgcggcct ccacggggtc cgccatcgcg ggctcggggg cccaacagcc
     1621 gctttaccag accgaacgct atgccgccac cttggaatac aagttcaacg tgcccaacgg
     1681 gctctatcaa gtgaccctaa agaacgccga gcagtactac accaccacgg ggaaccgcgt
     1741 cttcagcgtg gccatcaacg gcgtcacggt catcaccaac atggacctgg tggcgcaggt
     1801 cggggccttc acggccgacg accacacctt cgcggtctcg gtgaccggcg gcctcatcga
     1861 catcgtgggg acggccagca aggacaacgc cgagttcacg gccatcgagg tggtcagcga
     1921 agcgccctgt tcccccacgc cgacccccac gcccacccgg accgccaccc ggacgccgac
     1981 gccttccccg acggtgagcc ccacggtgag c
//
LOCUS       DASTJJ010000567         1396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_324230, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000567 DASTJJ010000000
VERSION     DASTJJ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324230"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..>1396)
                     /locus_tag="VFR02_06975"
     CDS             complement(243..>1396)
                     /locus_tag="VFR02_06975"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET9870218.1"
                     /translation="RSFDAEGLKAFTEGLLLGSYQYNDYKTQLKDAPAALGTLVLKAA
                     SGFLAQAKEAVKTGEAYAAGALLVRDMVNGPPSDTTPKLLVAKARRIAKPGSGIRLKV
                     YAKKDLVRMGAGGLLGVNRGSAEEPFLIHLHYKPAGKPKKSLALVGKGITFDSGGLSL
                     KPAGAMETMKMDMAGGAAVLGVFSQLAKFRPNVEVHGIVPTTENMPGDRAVKPGDVLK
                     AMNGKTMEVLNTDAEGRLILSDGLSYAVRQKPDEIIDMATLTGACVVALGSLVSGAMA
                     NDAGLLSRFKASTEAEGERIWELPLVKEYRDDIRSKVADIKNIGGGREAGSIIGGLFL
                     QEFVGETPWIHLDIAGPAYLEREYPALPYLTYGGTGLMVRSLLTYIRKG"
BASE COUNT          213 a          465 c          462 g          256 t
ORIGIN      
        1 tctcctggag cgcgtcctcg gcgtcctggg gcttgcccag catgcgccag gacaggcggt
       61 agaccttggt ccggtgcagg tcgtaaaggg cccggagccc ctcgatgcgg cccgaccggc
      121 aaaggttgat ggcctccgtc tctgtcattc cccacctggt attccctgga tttaagacgg
      181 ccgaagcggg cgggcggttg caaaaaagag gcgcttttcc cgctggaaaa gcgcccccgt
      241 cctcagcctt tgcggatgta ggtcaaaagg ctccgcacca tcagcccggt cccgccgtag
      301 gtcaggtagg gcaaggcggg gtattcccgc tccaggtagg cgggacccgc gatgtccagg
      361 tggatccagg gcgtttcccc cacgaattcc tgcaggaaaa ggcccccgat gatcgacccg
      421 gcctcccggc cgccgccgat gttcttgatg tcggccacct tgctgcggat gtcgtcgcgg
      481 tattccttga ccaggggcag ctcccagatg cgctccccct cggcctccgt ggaagccttg
      541 aaccgggaaa ggagccccgc gtcgttggcc atggcccccg acaccaagga tcccagggcc
      601 accacgcagg cgccggtcag ggtggccatg tcgatgatct cgtcgggctt ctgccgcacg
      661 gcgtaggaca ggccgtcgga caggatgagc cgcccttcgg cgtcggtgtt gagcacttcc
      721 atggtcttgc cgttcatggc cttgagcacg tcgccgggct tgacggcccg gtcgccgggc
      781 atgttctccg tggtgggcac gatgccgtgc acctccacgt tggggcggaa cttggcgagc
      841 tgactgaaga cccccaacac ggcggcgccg cccgccatgt ccatcttcat ggtctccatg
      901 gcgcccgcgg gcttgagcga cagtccgccc gaatcgaagg tgatgccctt gcccaccagg
      961 gccagggact tcttgggttt gccggccggt ttatagtgga ggtggatgag gaagggctcc
     1021 tccgcgctgc cccggttgac ccccaagagg ccccccgcgc ccatgcgcac caggtccttc
     1081 ttggcgtaga ccttcaaccg gatgccgctc ccgggcttgg cgatccggcg cgccttggcc
     1141 accagcaatt tgggggtcgt gtcgctgggc ggcccgttga ccatgtcccg caccaagagg
     1201 gccccggcgg cgtaggcctc accggtcttg acggcctcct tggcttgggc caggaaaccg
     1261 gacgcggcct tgaggaccaa ggtccccaag gcggcggggg catccttcag ttgggtcttg
     1321 tagtcgttat attggtagga gcccaggagc aggccttccg tgaaggcctt caacccctcg
     1381 gcgtcgaagg aacggg
//
LOCUS       DASTJJ010000568         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1424541, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000568 DASTJJ010000000
VERSION     DASTJJ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424541"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFR02_06980"
     CDS             complement(<1..251)
                     /locus_tag="VFR02_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870219.1"
                     /translation="MKKAPLLAIFILFLGQSISLASDTRVDSMGGLSLVATDEVDDIN
                     PFILGNPAGLALLPAQSRLDVAGEWIKEYFPPSGDQFHL"
     gene            391..1287
                     /locus_tag="VFR02_06985"
     CDS             391..1287
                     /locus_tag="VFR02_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870220.1"
                     /translation="MRKISIPFILVLALSGASILWAEEGTPVPVPPDLNNAAPITDLG
                     PVDLPALPDLDGTAPPSNTQAPSLPAEAPAPKAAPAAPAPVPTRVPTLAPTPVPTPIP
                     AAPQAPTAGPSSQAAMPAPAATGGLEDYFPWTQGASLKYEYLKPRPGSSVKGTRLVEC
                     VSREVMPNGTLHFTLRTTEGGRVVEDRYSLYDNKVQHTFAGKQALADHVILEWPVDGQ
                     PVEWKLPGGDGQTHAFKVFPGKEKVYQKVYPDCLIVVDKAGNSSRFEYYAKGIGLVAV
                     ETYGRGMKLIEDESLALVQTQP"
     gene            1428..>2193
                     /locus_tag="VFR02_06990"
     CDS             1428..>2193
                     /locus_tag="VFR02_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET9870221.1"
                     /translation="MANDIVIAFGGAAGDGNASAGNILAQASARQGLSVYAYNSYQSL
                     IRGGHSYLRIRISDKKVTNHGDQVTALVTLNQDSMNRHLQELVSGGVCIDNSDKLKAG
                     TPPAGVQMCPFPFYEIAGNDALPVMQNTVAIGVLTRMIGMDFSYLQSVFEHTFAKKPQ
                     VVEANVKAAKAGFEYAEKNFKPLPNPLGKTKEKLAFVTGNELLAMGAANAGCKVYCAY
                     PMSPASGILHWMGSHGKELGICVRQVEDEIGVINMTI"
BASE COUNT          431 a          724 c          670 g          368 t
ORIGIN      
        1 tagaggtgga actggtcgcc cgaaggcgga aaatattcct tgatccattc gcccgccaca
       61 tccaggcgcg attgggcagg cagcaaggcg agcccggcgg gattgcccag gatgaagggg
      121 ttgatgtcgt ccacctcgtc ggtggccacg agggaaagcc cgcccatgga atccacgcgg
      181 gtgtcggaag ccaagctgat gctttggccg aggaagagga tgaaaattgc caaaagcggc
      241 gcttttttca tgggtttacc ctgaaacaga atttttcgag tataccatgc ccgggggttc
      301 tccttccaaa aatggggcgc ccttgccgaa gggcgggaag ggtataatcc tgcctcaatg
      361 gcggagattc caacgcaaaa ggcggcgatc atgcggaaaa tttcgatccc cttcatcctg
      421 gttttggctt tatccggcgc ttccatcctg tgggctgagg agggtacgcc cgtgcccgtg
      481 ccgcccgatc tgaacaacgc ggcccccatc acggatttgg gcccggtgga cctgcccgca
      541 ttgccggacc tggacgggac cgcgccgcct tccaacacac aggccccttc gctgcccgcc
      601 gaagcgcccg cgcccaaggc ggcccccgcc gccccggcgc ctgtgccgac ccgggtgccc
      661 accttggcgc cgacaccggt ccccacgccc atcccggccg cgcctcaagc ccccacggcc
      721 ggcccatcct cccaggccgc catgccggcc ccggccgcca cggggggcct ggaggactat
      781 ttcccctgga cccaaggcgc cagcctgaaa tatgaatacc tcaaaccccg cccgggctcg
      841 tcggtgaagg gcacccggct ggtggaatgc gtttcccggg aggtcatgcc caatgggacc
      901 ctgcatttca ccctgcgcac caccgaaggc ggccgggtgg tggaggaccg ctattcgctt
      961 tatgacaaca aggtgcagca taccttcgcc gggaagcagg ccctggccga ccacgtcatc
     1021 ctggaatggc cggtggacgg acaaccggtg gaatggaagc tgcccggggg cgacggccag
     1081 acccacgctt tcaaggtctt tcccggcaag gagaaggtct accagaaggt ctatccggat
     1141 tgcctgatcg tggtggacaa ggccgggaat tccagccgct tcgagtatta cgccaagggc
     1201 atcggattgg tggcggtgga aacctacggc cggggcatga agctcatcga ggatgagtcc
     1261 ctggcgttgg tgcagaccca accctagagg gccgggagcc gcgcttttgg gccgaaatcc
     1321 ggcctttttc gccggaaaag tcttgcggcg gtgaaggcct cacgcttatg atgcataccc
     1381 tgacacgaca accccggtgg ggacttatct taggaggcaa gttttcaatg gctaacgaca
     1441 ttgtgatcgc attcggcggc gcggcgggcg acgggaacgc ctcggccggc aacatcctgg
     1501 cccaagccag cgcccggcag ggcctgtcgg tgtacgctta caacagctac caatccttga
     1561 tccgcggcgg ccattcctac ctgcgcatcc gcatctccga caagaaagtc accaaccacg
     1621 gcgaccaagt gaccgccctg gtcaccctga accaggattc gatgaaccgc cacctgcagg
     1681 aactggtgtc cggcggcgtc tgcatcgaca acagcgacaa gctcaaggcc ggcaccccgc
     1741 ccgcgggcgt gcagatgtgc cccttcccct tctatgagat cgccggcaac gacgccctgc
     1801 ccgtcatgca gaacaccgtg gccatcggcg tgctgacccg catgatcggc atggacttct
     1861 cctacctgca gtcggtgttc gagcacacct tcgccaagaa gccccaagtg gtcgaggcca
     1921 acgtcaaggc cgccaaggcc ggcttcgaat acgccgagaa gaacttcaag cccctgccga
     1981 accccctggg caagaccaag gagaagttgg ccttcgtcac cggcaacgag ctcttggcca
     2041 tgggcgccgc caacgcgggc tgcaaggtct attgcgccta tcccatgagc ccggcctcgg
     2101 gcatcctgca ctggatgggg tcgcacggca aggaactggg catctgcgtg cgccaggtcg
     2161 aggacgagat cggcgtcatc aacatgacca tcg
//
LOCUS       DASTJJ010000569         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1669968, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000569 DASTJJ010000000
VERSION     DASTJJ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669968"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFR02_06995"
     CDS             complement(<1..231)
                     /locus_tag="VFR02_06995"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9870222.1"
                     /translation="MRPGASQKGSPVRVVGLTGGMGAGKSEVLRLIARRGFPVLQADQ
                     VGHALLRDRRFARRLEKRFGPGLLDGGGGVNRR"
     gene            complement(228..1076)
                     /gene="folD"
                     /locus_tag="VFR02_07000"
     CDS             complement(228..1076)
                     /gene="folD"
                     /locus_tag="VFR02_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET9870223.1"
                     /translation="MPLILDGKAAAQAVRDGLKAEMEKLPPGSRPGLAVVLVGDNPAS
                     LVYVGMKKKACEALGYHSEEVKLPKETTQDQLAAVIDRLNRDPKVHGILVQLPLPKHL
                     STDRVIDAIDPRKDVDGLHPANLGKLLAGEKGLRPCTPLGVMTLLDHFRVPLAGKRAA
                     VIGRSNLVGKPLGLLLLEADATVTLCHSKTAGLGDVLRQADLVVAAIGRPLFVKADMI
                     KPGAVVVDVGTSKGGDGRLLGDVDYGPVSQVASAISPVPGGVGPMTIALLLRNTFQAF
                     RDQTAP"
     gene            complement(1154..2011)
                     /locus_tag="VFR02_07005"
     CDS             complement(1154..2011)
                     /locus_tag="VFR02_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9870224.1"
                     /translation="MSAPLFLDGRLVPEARAAVSPLSSAALFGESLFESLPVYGGKPL
                     FLDDHLGRLRKGCAFLGWPMPPAARFRRAIGLFHRVHSGDFIIRFNLAREYRMPVHPR
                     TRGEGPPRLWAFIRPLTHGPGDFWPLLGPVGVSPWRVPDRQSAPNQFKWAFYMMTRRA
                     YHAHPGWKEMVRVNAQGYAVDGAGSGVMWFDGKKVRVPPRAWVGLESVTQNRVRALCG
                     RLGVRVVERPWRPRETLRRGELIFTGSGVGVMGATHLNGARLPGPPRLALRLWQYYRD
                     WARDPDGAS"
     gene            complement(2008..>2721)
                     /locus_tag="VFR02_07010"
     CDS             complement(2008..>2721)
                     /locus_tag="VFR02_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9870225.1"
                     /translation="EGEPLSLYRILREVNPGPFMGLLAAPSFTLVSSSPERLVRGRGG
                     LLETRPIAGTRPRGKDPEEDLRLKQELRSHPKELAEHLMLVDLMRNDLGRVARYGTVE
                     VADYARVESYAKVHHLVSTVTARARDDARFSHIFRSVFPGGTITGCPKVRCLQVIDEL
                     EREPRHFYTGSLGYLAPGGLFDLNILIRSFTLWPGGEMEFFAGAGIVADSDPDREYAE
                     TLHKVEALAQALGTRLAPL"
BASE COUNT          450 a          977 c          908 g          386 t
ORIGIN      
        1 ccggcggttc acgcccccgc ccccgtccaa aaggccggga ccgaagcgct tttccagccg
       61 ccgggcgaac cggcggtccc gcagcagggc gtgtcccact tggtccgcct ggagcacggg
      121 aaaaccccgg cgggcgatca agcgcaacac ctcgctcttc cccgccccca tgcccccggt
      181 caggcccacc acccggaccg gggacccttt ctgggacgcc ccgggtctca aggagcggtc
      241 tggtcgcgga acgcctggaa ggtgttccgc aaaagcaggg cgatcgtcat gggccccacg
      301 cccccgggca ccgggctgat ggccgaggcc acctgggaca cgggcccata gtccacgtcg
      361 cccaagagcc tgccatcgcc ccccttggag gtgcccacgt ccaccaccac ggccccgggc
      421 ttgatcatgt cggccttcac gaaaaggggg cggccgatgg cggccaccac caggtccgcc
      481 tggcgcagca cgtcccccag gccggccgtc ttgctgtggc aaagggtcac ggtggcgtcg
      541 gcctccagca acaaaagccc caggggtttg cccaccaggt tgctgcggcc gatgaccgcc
      601 gcccgcttgc ccgccagggg gacgcggaaa tggtccaaca gggtcatgac ccccaggggc
      661 gtacagggcc tcagcccctt ctcgccggcc agcagcttcc ccaggttggc cggatgcagg
      721 ccgtccacgt ccttgcgggg gtcgatggcg tcgatgaccc ggtccgtgct caagtgcttg
      781 ggcaggggca gttggaccag gatgccgtgg accttggggt cgcggttcaa gcggtcgatg
      841 accgcggcca attggtcctg ggtggtctcc ttgggcagct tgacctcttc cgaatggtac
      901 cccagggcct cgcaagcctt cttcttcatc cccacataga ccagggacgc cgggttgtcg
      961 cccaccaaca ccaccgccaa gccgggcctg gagcccgggg gcagtttttc catctcggcc
     1021 ttgagcccgt cccgcacggc ctgggcggcg gccttcccat ccaagatcaa aggcataggt
     1081 tccctccggc ttttgtcagc cggtcatttt atccgtccgg cccggccaaa caaagccgaa
     1141 accaccccgc ccctcacgaa gcaccgtccg ggtcgcgcgc ccagtcccgg taatactgcc
     1201 aaagccgaag ggccaagcgg gggggacccg gcaggcgcgc gccgttgagg tgggtggcgc
     1261 ccatcacacc cacgcccgat ccggtgaaga tcagttcccc ccggcgcaag gtttcccggg
     1321 gacgccaggg ccgctcgacc acccgcaccc ccagccgccc gcagagggcc cgcacccggt
     1381 tttgggtgac gctttcaagc cccacccagg cgcgcggggg cacccggacc tttttcccgt
     1441 cgaaccacat cacgcccgat cccgccccat ccacggcgta gccttgcgcg ttcacccgga
     1501 ccatttcctt ccagcccgga tgggcgtggt aggcccgccg ggtcatcatg tagaaggccc
     1561 atttgaactg gttgggcgcg ctttgccggt ccggcacccg ccaggggctc acgcccaccg
     1621 gccccaggag gggccaaaaa tccccggggc cgtgggtcag gggacggatg aaagcccaga
     1681 gccggggagg gccttccccc cgggtgcggg gatggaccgg catgcggtat tcgcgcgcca
     1741 ggttgaaacg gatgatgaaa tcgccggaat ggacccggtg gaaaagcccg atggcccggc
     1801 ggaagcgggc ggccggcggc atgggccagc ccaaaaaggc gcatcccttg cgcaaccgcc
     1861 ccaggtggtc gtccaggaaa aggggctttc ccccatagac cggcaggctc tcgaacaggc
     1921 tctccccgaa aagggccgcg ctggaaaggg gcgacaccgc cgcgcgcgcc tcgggcacca
     1981 ggcgtccgtc gaggaaaaga ggggcgctca taggggggcc agccgcgtgc ccaaggcctg
     2041 ggccagggcc tcgaccttgt gcagggtctc ggcgtattcc cggtccgggt ccgaatcggc
     2101 cacgatgccc gcgccggcga agaactccat ctcccccccg ggccagaggg tgaagctgcg
     2161 gatgaggatg ttcaggtcga acagcccccc cggggccagg tagcccaggc taccggtgta
     2221 gaaatggcgg ggttccctct cgagctcgtc gatgacctgc aggcagcgca ccttggggca
     2281 acccgtgatg gtgccgcccg ggaagaccga ccggaagata tgggagaacc gggcgtcgtc
     2341 ccgcgcccgg gccgtgaccg tggagaccag gtgatgcacc ttggcgtagg attccacccg
     2401 ggcgtagtcc gccacttcca ccgtgccgta acgggccacc cggcccaggt cgttgcgcat
     2461 caggtccacc agcatgaggt gctcggccaa ttccttggga tggctgcgca gttcctgctt
     2521 caagcgcagg tcctcctcgg ggtccttgcc ccggggacgg gtgccggcga tgggccgggt
     2581 ctccaggagc ccgccccggc cccggaccag gcgctcgggc gaggaggaca ccagcgtgaa
     2641 agaaggcgcg gccaggagac ccatgaaggg gccgggattg acctcccgca ggatccggta
     2701 caaggacagc ggctcgcctt c
//
LOCUS       DASTJJ010000570         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1424655, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000570 DASTJJ010000000
VERSION     DASTJJ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424655"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..616)
                     /locus_tag="VFR02_07015"
     CDS             complement(134..616)
                     /locus_tag="VFR02_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870226.1"
                     /translation="MPTQDPEGPPVGPARDGEVPFGAFRSALSDYADLACEPIGAPVE
                     GPAVMSLERSIRLSGPIRGLIVVRAHRRLGGLLLERAAGGNGHSDSREAAFDYLVRLF
                     AEKWVRSRWALGDFDPLTTDISSPQVWPLGPPRDSAALLVEHCPVEIRIWWETKEARP
                     "
     gene            complement(784..>1037)
                     /locus_tag="VFR02_07020"
     CDS             complement(784..>1037)
                     /locus_tag="VFR02_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9870227.1"
                     /translation="MVIGARGSRVGFADLPDEVAAYEPGAPEAAPDEEPLKVPQGSTE
                     KEAVERALHQSRWNKSKAAELLGVTRKTLFNKMKKYDIH"
BASE COUNT          168 a          356 c          349 g          164 t
ORIGIN      
        1 agggcggccg cgcgcgaaag ccaggcttcc agttcggccg cgccccgggc cgcgccggcc
       61 ccttgggcca ggcgcgccga gatctcggcc agttccaggc ccaatccccg ttggaccgcc
      121 tcccactcct cgttcatgga cgcgcctcct tcgtttccca ccaaatgcgt atctccaccg
      181 ggcaatgctc caccaaaagg gccgccgaat cccgcggcgg ccccagcggc cagacctggg
      241 ggctcgagat atcggtggtc agggggtcga aatcgcccaa ggcccagcgg gaccggaccc
      301 atttctcggc gaagagccgg accaaatagt cgaaggccgc ctcccgggaa tccgaatggc
      361 cgttgccgcc ggccgcccgt tccaacaaca gcccgcccag gcgccggtgg gcccgcacca
      421 cgatcagacc ccggatgggc ccggacaacc ggatgctcct ttcgagcgac atgaccgccg
      481 gcccctccac cggcgcgccg atgggttcac aggccaggtc ggcgtagtcc gaaagcgcgg
      541 accggaacgc cccgaaaggg acctccccgt cccgggcggg tccgaccggg gggccttcgg
      601 gatcctgggt gggcatggga tctttccttc gttgggatgg cgcgggcttt gtccgtccct
      661 ggggtagggc aaaggccgtg ccaggaagct ttgttgaaaa gaaaggggat ccggaaaggg
      721 aaagcgggat cccttaccca acttgggtaa gggcggggac aaactgggta aggcgcggga
      781 ccgtcagtgg atgtcgtact tcttcatttt gttgaagagc gttttgcggg tgacgcccag
      841 gagctcggcg gccttggact tgttccaacg ggactggtgc agggcccgct caacggcttc
      901 cttctccgtg ctgccctggg ggaccttcaa gggttcctcg tccggggccg cttcgggggc
      961 gccgggctca tacgcggcga cctcgtcggg caggtcggca aatcccaccc ggctgccccg
     1021 ggccccgatg accatcc
//
LOCUS       DASTJJ010000571         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1424813, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000571 DASTJJ010000000
VERSION     DASTJJ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424813"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFR02_07025"
     CDS             complement(<1..1150)
                     /locus_tag="VFR02_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9870228.1"
                     /translation="MTETTHFLQDLAMVLCVAAVTTLLFQRLRQPPVLGYLLAGLILG
                     PHVHLPLYADTDTVRVLANLGVILVMFSIGLTFTIERLVRVLPTAGLTILLEVGVMAW
                     LGYGAALLMGWTPLEGVFTGAMLSISSTMISSKALADQQADEKLSRAVVSVLVLQDLA
                     AVLLVAVLTPMARGARLSINALATTSGTLLAFLSVLFVAGYLIIPRVIRLALRLRNPE
                     TLLVTAVGICFAMALLAEKGGYSVALGAFLAGMLVAESGATLTLERLIHPLRDMFAAI
                     FFVAIGMLVDPRVLLGHWRAVVFLTLLVIAGQIASVSVGAFLSGRPIRTAIQAGMSLA
                     QIGEFSFILAQVGTSTGAISGFLYDVAVAVSVVTAFTTPWLIRAAGPVS"
     gene            complement(1217..>1397)
                     /locus_tag="VFR02_07030"
     CDS             complement(1217..>1397)
                     /locus_tag="VFR02_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870229.1"
                     /translation="CFWDPSLHKTKTIGTLMEADKARFFKNQISSILIIVPKACFYSF
                     GEGPGSVLFLGYFRP"
BASE COUNT          294 a          420 c          492 g          191 t
ORIGIN      
        1 gggacaccgg gccggcggcc cggataagcc agggggtggt gaaggccgtg accaccgaga
       61 cggccaccgc cacgtcgtac aagaagccgc tgatggcgcc cgtggaggtg cccacctggg
      121 ccaagatgaa ggagaactcg ccgatctggg ccaggctcat gccggcctgg atggcggtcc
      181 ggatggggcg cccgctcagg aaggccccca cggacacgct ggcgatctgg cccgcgatga
      241 ccagcaaggt gaggaacacc acggcccgcc agtgccccaa cagcacccgg gggtccacca
      301 gcatgccgat ggccacgaaa aagatggcgg cgaacatgtc ccgcaggggg tggatgagcc
      361 tctccagggt gagggtggcg cccgattcgg ccaccaacat gccggccagg aaggcgccca
      421 aggccaccga gtaaccgccc ttctcggcca agagggccat ggcgaagcag atgcccacgg
      481 cggtcaccag cagggtctcg ggattgcgca ggcgcaaggc caggcggatg acccggggga
      541 tgatgaggta gcccgccacg aaaagcacgc tcaggaaggc cagcagggtg ccgctggtgg
      601 tggccagggc gttgatggaa aggcgcgcgc cccgggccat gggggtcagg acggccacca
      661 gcagcaccgc cgccaggtcc tgcagcacca gcacgctcac cacggcccgg gacagcttct
      721 cgtcggcctg ctggtccgcc agggccttgg aggagatcat ggtgctggag atggacagca
      781 tggccccggt gaagacgccc tccaggggcg tccagcccat gagcagggcc gcgccgtagc
      841 ccagccaggc catcaccccc acttccagca ggatggtcag cccggcggtg ggcagcaccc
      901 gcaccagccg ctcgatggtg aaggtgagcc cgatggagaa catgaccagg atgacgccca
      961 ggttggccaa gacccgcacg gtgtcggtgt cggcgtagag gggcaggtgc acgtgggggc
     1021 ccaggatgag cccggccagc aggtaaccca ggaccggggg ctggcgcagg cgctggaaca
     1081 ggagggtggt gacggcggcc acgcagagca ccatggccag gtcctgcagg aagtgggtgg
     1141 tctcagtcat ggctgggctc gttcaagtcg cggcctcggg gtggaatggg gtgtggtcgg
     1201 gcctattttc cctttttcag ggccgaaagt aacccaaaaa caaaacggaa ccgggccctt
     1261 caccaaacga ataaaagcag gcttttggaa caatgatgag gatggatgaa atctgatttt
     1321 tgaaaaatcg ggctttatca gcttccatca atgttccaat tgtctttgtc ttgtgaagtg
     1381 aaggatccca aaaacaa
//
LOCUS       DASTJJ010000572         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_567864, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000572 DASTJJ010000000
VERSION     DASTJJ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567864"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFR02_07035"
     CDS             complement(<1..660)
                     /locus_tag="VFR02_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870230.1"
                     /translation="MTPVLLFLAPFLAAFLAGLLAWGGRRASRAAALLGAAAALGLAL
                     AAGFQSLGGDLAYEMGGWAAPWGIEIRLTPFACFWTAALDALCLAALALAPREGAARP
                     DPFDAVLLLLTGSAAALVLDRDLFDAYLWVELALLGAGLLAAREGSPWTAFRIAFWGS
                     VGASFLLLGVLFLYSGTGTLDLGDLLSQLLILQDAAPKNFRALIGFGGFWLTLGLALP
                     LM"
     gene            complement(657..959)
                     /locus_tag="VFR02_07040"
     CDS             complement(657..959)
                     /locus_tag="VFR02_07040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HET9870231.1"
                     /translation="MSPLLALAALAAGGTGLYGVLTQTRLSRKIAAWALVQAGLALLP
                     LALAAAHPNALSPALALDILSVSLALGLFFWRLAASLRRDKGTEDEKLLSHLEEAS"
     gene            complement(956..1516)
                     /locus_tag="VFR02_07045"
     CDS             complement(956..1516)
                     /locus_tag="VFR02_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870232.1"
                     /translation="MPKNRFFPRRRNRPSPGARWLSALAVLCLGAFGWAGFHALSPAP
                     GIPSPLTSHVALRYAERGPLETGGSHPTLAVFADYRALDPAVTALVFMAAAFVLFLPG
                     PDPKGRKSAIDWPGLWVFAGPCLAWGLGLACLAGGANFLDYDGLRLPLPSGDIRAAGA
                     ALVGAGLLLAALFLTLPRAFGQGRKP"
     gene            complement(1628..>1976)
                     /locus_tag="VFR02_07050"
     CDS             complement(1628..>1976)
                     /locus_tag="VFR02_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9870233.1"
                     /translation="LTVPLVGFLTAVLFGSLGLFAVRLVKDISQFNFFISGVISPLTL
                     FSGTLFPVDKLPKAVAFCAYWLPLFHPVHLTRMLTTGRFEADWPLSLAYTLGVPWVLG
                     FFAVKSIRPKLIQ"
BASE COUNT          410 a          646 c          729 g          191 t
ORIGIN      
        1 catcaggggc aaggccagcc ccaaggtcag ccaaaagccc ccgaagccga tgagggcccg
       61 gaaatttttc ggggccgcgt cctggaggat gagcaattgg gacaagaggt cccccaggtc
      121 caacgtgccc gtgcccgaat agaggaaaag gacgcccaag agcaggaagg aagcgcccac
      181 gctcccccaa aaggcgatcc ggaaggcggt ccagggggag ccttcccggg ccgccagcag
      241 cccggcgccg agcagggcca gttccaccca aaggtaggcg tcgaaaaggt cccggtccag
      301 gaccagggcc gccgcgctgc cggtgagcaa cagcaggacc gcgtcgaaag gatcggggcg
      361 ggcggcccct tcccgggggg ccaaagccag cgcggccagg caaagggcgt ccagggccgc
      421 ggtccagaaa caggcgaagg gcgtgaggcg gatctcgatg ccccaaggcg cggcccagcc
      481 ccccatctca taggccaagt cccctcccag gctctggaag ccggcggcca gggccagccc
      541 gagggccgcg gccgccccca agagggccgc cgcgcgggac gcccggcggc cgccccaggc
      601 caggagcccc gcgaggaagg ccgccaggaa gggggcgagg aagagcaaga ccggggtcaa
      661 gacgcttcct ccagatggga caacagcttt tcatcttccg tccccttgtc ccggcgcagg
      721 gaggccgcca accgccagaa gaaaagcccc aaggccagcg agacggacag gatgtccaag
      781 gccagggcgg gggacaaggc gttgggatgg gccgccgcca gggccagcgg caagagggcc
      841 aagccggcct ggaccagggc ccaggcggcg atcttacggg acaaccgggt ctgggtgagg
      901 acgccgtaaa ggccggtccc gcccgccgcc agggccgcca aggccaacaa ggggctcatg
      961 gttttcgccc ttggccgaag gcccggggca gggtcaggaa aagcgcggcc agcagcagcc
     1021 ccgcgccgac cagcgccgcg ccggccgcgc ggatgtcccc ggaaggaagg ggaagccgca
     1081 agccgtcgta gtccaggaaa ttcgcgccgc ccgccaggca ggccaggccc aggccccagg
     1141 ccaggcacgg accggcgaag acccacaagc ccggccaatc gatggcgctc ttgcggcctt
     1201 tgggatccgg tccgggaagg aacagcacga aggccgccgc catgaagacc aacgccgtca
     1261 cggccgggtc caaggcgcgg tagtcggcga agaccgccag ggtcggatgg gatcccccgg
     1321 tctccagcgg gccgcgctcg gcgtagcgca aggccacgtg ggacgtcaaa ggggagggga
     1381 tgccgggcgc cggggacagg gcgtggaagc cggcccaccc aaaggccccc aggcaaagga
     1441 cggccaaggc cgagagccac cgtgccccgg gggagggcct gttgcggcgc cgtggaaaaa
     1501 atctgttctt gggcatgtgg atctttccga agcgtccgat ttgggccgac cggggcttat
     1561 cctagtgagc gcgctggggg ccgtcaatct tcaaaccggg aagccggagg gacggggccc
     1621 ggaagggcta ttggatcagc ttgggccgga tggacttcac ggcgaaaaaa cccagcaccc
     1681 agggcacccc cagcgtatag gccaggctca ggggccaatc ggcctcgaag cggccggtcg
     1741 tgagcatgcg ggtcaggtgc acggggtgga acagcggcag ccagtaggcg cagaaggcca
     1801 ccgccttggg cagcttgtcc accgggaaaa gcgtgccgga gaaaagcgtc aggggcgaga
     1861 tgacgcccga gatgaagaag ttgaactggg agatgtcctt gaccagccgc acggcgaaaa
     1921 gccccaggct gccgaacagc acggccgtca ggaagcccac caggggcacg gtgagc
//
LOCUS       DASTJJ010000573         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1670227, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000573 DASTJJ010000000
VERSION     DASTJJ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670227"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFR02_07055"
     CDS             complement(<1..281)
                     /locus_tag="VFR02_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9870234.1"
                     /translation="MEPPKYPVGPAPAQDPPDAAGREPLIAQIEEAPALLRRALEGLG
                     EDRLGVKYKNWTVRQIANHLADSHVNSYVRFRWTLTEETPLIKAYDENL"
     gene            complement(305..2314)
                     /locus_tag="VFR02_07060"
     CDS             complement(305..2314)
                     /locus_tag="VFR02_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870235.1"
                     /translation="MPRKRSTSISRPVTKVLPDGTVSPLPVPRTAPPSGPLGRWMQQG
                     PRVEEQAPSGGHPWHRVLWLTGVDYFSSLGYQPGIALLAVGALSPLATLVLVAVTLCF
                     AMPMYRQVASRSFAGQGSVAMLENLLDGWWGKGVVLVLLGFACTAFVITMTLSSADAA
                     VHLTENPLLAPWLRGHTTALTLLVLAVLAWIFLMGFKEALGVARLVALPYLALNLVVI
                     GRCAWEAVRDPGLWSHWTGSLSSLGDPAGLLLVSALAFPQLALGLSGFETGVAVMPQV
                     DGGPGDKGQPLPWGRVRATRRLLAAAALIMSFLLAASSLVTVLLVPPEAYRDGGPAAG
                     RALAWLAHRYLGEGFGSLYDLSTILILGFAGASAMTGLLNLIPRYIPRFGMAPRWILH
                     TRPLTLALFGVNVLVTLAFHADVERQAGAYATGVLSLMLSASVAVALAFARESRGEPG
                     RRKWAGTAYFTLASAVFLYALVDNLLSRPDGLVIALLFVAAILVLSGLSRTVRSSELR
                     VADIRLEDARSIRLFKAVRGRKVNLVPLRSGDAEARRSKERWIRRHHRVEGPLVFLIV
                     RLMDNRSEFLASLRMRLRKEGPHYVMTVSGAVAVANTIAYVSELIDPKSLFLTLTRQD
                     LMTQSFRYVLWGEGEVGLDVYSTLVKYWSWTPPGDVRPRLVLVSN"
     gene            2430..3770
                     /locus_tag="VFR02_07065"
     CDS             2430..3770
                     /locus_tag="VFR02_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9870236.1"
                     /translation="MSPAAPPPRSPLKYLGDGLSGLPWASDRNGLYIKAVQLAAAVLV
                     GVLSYGYAGLISATQGFFLFAFHGHTWLAALAAPLVFLGSTWLVVRFAPEAHGSGIPQ
                     VLRIIEDSDRPRSVLNPLVSVKTAAFKALSTALGTLGGASIGREGPTVQIAASAFAFA
                     AQKARLYFGLADFRSFLVAGAAAGIAAAFNTPLAGITFALEEIAEATFTQFKRVVMLS
                     VIVAGITAQALGGNYLYFGHPLIPVLSSSILPFALVIGLAGGLGGGFFARVLTSPFNG
                     LLPRRWWTRALACGVFCSALALLTHGVTTGSGYEATRHFMDDPDGRLSPLFFAEKFLA
                     TVFSYMSGMAGGIFSPCLSIGAGMGFTLAHLFHVPDLRACALIGMVAFFSGVVQTPLT
                     AVIIITEMTDQSSLLIPFLIAAFVAHGVGKLIMPVPLYRTLANRAAALADPPSA"
     gene            complement(3774..>4695)
                     /locus_tag="VFR02_07070"
     CDS             complement(3774..>4695)
                     /locus_tag="VFR02_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870237.1"
                     /translation="VELPVLDITHPAFRPPEGAGPGPWFAFLKQLPPFAWVREKLGPS
                     SVLQQGLRRARGGFLSGMDTYLFKLGPDNLGRAYAGARDRKMAGGALARSLALRTAAM
                     ARMLAQGLAPQLRARPGRPLDLINLGGGPAMDSLNALILLAQKSPRALRGRRVTIHVL
                     DLEAEGPDFGARALAALQKPGGPLAGLRARLRYVRYDWAAPAPLLALLRRCAAGKGVM
                     AGSAEGSLFSYASDGQIAEHLALFQAATPASFRMVGSLAIVGPGPGPALRDRGPEELA
                     ALARSGGWRARFTGGSAFSRHFYLEKTGKG"
BASE COUNT          717 a         1689 c         1594 g          695 t
ORIGIN      
        1 cagaggttct cgtcataggc cttgatgagc ggggtctcct cggtgagcgt ccaccggaac
       61 cgcacgtagc tgttcacgtg gctgtcggcc aggtggttgg cgatctggcg gaccgtccag
      121 ttcttgtact tcacgcccag ccggtcctcc cccagccctt ccagggcccg gcgcaggagc
      181 gcgggggcct cctcgatctg ggcgatgagc ggctcccgtc cggcggcgtc cggcgggtcc
      241 tgcgcggggg cgggaccgac gggatatttg ggtggttcca tggctttccc tttcaacggc
      301 tcgttcaatt gctcaccagc accagccggg gacgcacgtc gcccggcggg gtccaggacc
      361 agtacttgac cagggtcgaa tagacgtcca gccccacctc gccctcgccc cagagcacgt
      421 agcggaagga ctgggtcatg aggtcctgcc gggtgagggt gaggaacagg ctcttggggt
      481 cgatgagttc gctgacgtag gcgatggtgt tggccaccgc caccgccccg ctgaccgtca
      541 tgacgtaatg gggcccttcc ttgcgcaggc gcatgcgcag cgaggccagg aactcgctgc
      601 ggttgtccat gagccgcacg atgaggaaga ccaggggccc ctccacccgg tggtggcggc
      661 ggatccagcg ttctttggaa cggcgggcct cggcgtcgcc cgagcgcagc ggcaccaggt
      721 tgaccttgcg gccccgcacg gccttgaaga gccggatgga ccgggcgtcc tccagccgga
      781 tgtcggccac ccgcagttcg ctggagcgca cggtgcggct caagccgctc aagaccagga
      841 tggccgccac gaacaggagc gcgatgacca gcccgtcggg ccgggaaagc aggttgtcca
      901 ccagggcgta gaggaacacc gcgctggcca gggtgaaata ggcggtcccc gcccatttgc
      961 gccggcccgg ttccccgcgg gattcccggg cgaaggccag cgccaccgcc accgaggccg
     1021 acagcatgag cgacaggacc ccggtggcgt aggcccccgc ctggcgttcc acgtcggcgt
     1081 ggaaggccag cgtgaccagg acattgaccc cgaaaagcgc cagggtgagg gggcgggtgt
     1141 gcaggatcca ccggggcgcc atgccgaacc gggggatgta gcgggggatc aggttgagca
     1201 gcccggtcat ggccgaggcc cccgcgaagc ccaggatgag gatggtggac aggtcgtaga
     1261 ggctgccgaa accctcgccc aggtagcggt gggccagcca ggccagggcc cggcccgcgg
     1321 cgggaccgcc gtcccggtag gcttcgggcg gcaccagcag cacggtgacc aggctggagg
     1381 ccgccaagag gaagctcatg atgagcgcgg cggccgccaa caggcggcgg gtggcccgca
     1441 cccggcccca gggcaggggt tgccccttat ccccggggcc gccgtccacc tggggcatga
     1501 ccgccacccc ggtctcgaaa ccgctgagcc ccaaggccag ttgggggaag gccagggccg
     1561 agaccagcaa cagcccggcc ggatcgccca gggacgaaag ggaccccgtc caatgggacc
     1621 agaggccggg gtcccggacc gcttcccaag cgcaacggcc gatcaccacc aggttcaagg
     1681 ccaggtaggg cagggccacc agccgggcca cgcccagcgc ctccttgaaa cccatcagga
     1741 agatccaggc caagaccgcc aggacgagga gcgtgagggc ggtggtgtgg cccctgagcc
     1801 agggcgccag cagcgggttc tcggtcaggt gcacggcggc gtccgccgag gagagcgtca
     1861 tggtgatgac gaaggccgtg caggcgaaac ccaaaagcac cagcaccacg cccttgcccc
     1921 accagccgtc gagcaggttc tccagcatgg ccaccgagcc ctggcccgcg aaggaacggg
     1981 acgccacctg ccggtacatg ggcatggcga agcagagcgt cacggccacc agcaccaggg
     2041 tggccagggg cgacagcgcg cccaccgcca agagcgcgat gcccggttgg tagcccaggg
     2101 acgagaaata atccacgccc gtcagccaca acacccggtg ccagggatgt ccccccgacg
     2161 gcgcctgttc ctcgacccgg gggccctgct gcatccaacg gcccaagggg cccgaaggcg
     2221 gcgcggtgcg cggcacgggc agcggcgaaa ccgtgccgtc gggcaatact ttggtcacgg
     2281 gacgggaaat ggaggtggat cttttccttg gcatcggcgg ggcggcgtcc tttcccttgg
     2341 gaattccagg ccccaagctt acaataaacc gggtcggccc ttcacgggcc cgcgccatta
     2401 ttttcgccac cccgacggag aaagccccca tgtccccagc cgcgcccccg ccccggtccc
     2461 ccctgaagta cctgggggac ggcttgtccg gcctgccctg ggcctcggac cgcaacggcc
     2521 tctatatcaa ggcggtgcag ctggccgccg ccgtgctggt gggcgtgctc agctacggct
     2581 acgccgggct catttcggcc acccagggtt tcttcctttt cgccttccac gggcacacct
     2641 ggttggcggc gctggccgcc cccctggtct tcctggggtc cacctggctg gtggtgcgct
     2701 tcgcgcccga ggcgcacggc agcggcatcc cccaggtgct gcgcatcatc gaggattcgg
     2761 accggccccg gtccgtcctc aaccccctgg tatcggtgaa gaccgccgcc ttcaaggccc
     2821 tgtccaccgc cttgggcacc ctggggggcg cctccatcgg ccgcgagggg cccacggtcc
     2881 agatcgccgc ctcggccttc gccttcgcgg cccagaaggc caggctttat ttcggcctgg
     2941 cggatttccg gtccttcctg gtggcgggcg cggcggcggg catcgcggcg gccttcaaca
     3001 cgcccttggc cggcatcacc ttcgccctgg aggagatcgc ggaggccact ttcacccagt
     3061 tcaagcgggt ggtcatgctc tcggtcatcg tggccgggat cacggcccag gccctgggcg
     3121 gcaactacct ctatttcggg cacccgctca tcccggtgct gtcctcttcc atcctgccct
     3181 tcgccttggt gatcggcctg gcgggcgggc tgggcggcgg cttcttcgcc cgggtgctca
     3241 ccagcccctt caacggcctc ttgccccggc gctggtggac ccgggccctg gcctgcgggg
     3301 tcttttgctc ggccctggcc cttctcaccc acggcgtcac caccgggtcg ggctatgagg
     3361 ccacccggca tttcatggac gaccccgacg gacggctgtc ccccctcttt ttcgccgaga
     3421 agttcctggc cacggtcttt tcctatatgt cgggcatggc cggggggatc ttctccccct
     3481 gcctgtccat cggggcgggc atgggcttca cgctggccca tcttttccat gtcccggacc
     3541 tgcgggcctg cgcgctcatc ggcatggtgg ccttcttctc gggcgtggtg cagacgcccc
     3601 tgaccgcggt catcatcatc accgaaatga ccgaccaaag ctcgctcttg atccccttcc
     3661 tcatcgcggc cttcgtggcc cacggggtgg gcaagctgat catgccggtc ccgctctacc
     3721 gcaccctggc caaccgggcg gcggccctcg cggacccgcc ctccgcctga ccctcagcct
     3781 ttcccggtct tttccaggta aaagtgccgg ctgaaggcgg accctcccgt gaatctcgcg
     3841 cgccatcccc cgctccgggc cagggcggcc agttcctccg gcccccggtc ccgcagggcg
     3901 gggccaggcc ccgggcccac gatggccaag gaacccacca tgcggaagga agcgggcgtg
     3961 gcggcctgga acagggccaa atgttcggcg atctgtccgt cggacgcgta ggaaaaaagc
     4021 gaaccctccg ccgaaccggc catcaccccc ttccccgccg cgcaccgccg cagcagggcg
     4081 agcaggggcg cgggcgcagc ccagtcgtaa cggacgtaac gcaggcgggc ccgcaacccc
     4141 gccaagggcc cgccgggctt ttgaagcgcc gccagggcgc gcgcgccgaa atccggcccc
     4201 tccgcctcca ggtcgagcac gtggatcgtg acccgccttc cccgaagcgc gcgcggggat
     4261 ttttgggcca ggaggatcaa ggcgttcaaa ctgtccatgg cgggcccgcc tcccaggttg
     4321 atcaggtcca ggggccgccc cggccgggcc cggagttgcg gcgccagccc ttgggccagc
     4381 atccgggcca tggccgccgt ccgcagcgcc agggaccggg ccaaggctcc ccccgccatc
     4441 ttccggtcgc gggcccccgc gtaggcgcga cccaggttgt ccgggcccag cttgaaaagg
     4501 taggtgtcca tccccgacag gaatcccccc cgggcccgcc gaaggccctg ttggaggacg
     4561 gacgacggcc ccagtttttc ccggacccac gcgaacggcg ggagttgctt gaggaaggca
     4621 aaccacggcc ccgggcccgc gccttcgggt ggacggaagg ccggatgggt gatgtccaac
     4681 acgggcagtt ccacc
//
LOCUS       DASTJJ010000574         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1058365, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000574 DASTJJ010000000
VERSION     DASTJJ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058365"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFR02_07075"
     CDS             <1..363
                     /locus_tag="VFR02_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9870238.1"
                     /translation="ELDLEGLKKIAPKTRQIRDFVTEYKFKNGKKIFVLGEGRLINLA
                     AAEGHPASVMDMSFANQALGAEYVVKNHNILDRKVYSVPVETDQEIARLKLKALGIKI
                     DTLTAEQEHYLSSWEMGT"
     gene            518..961
                     /locus_tag="VFR02_07080"
     CDS             518..961
                     /locus_tag="VFR02_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9870239.1"
                     /translation="MVPTSAIFAHLRGVLQRSYIMQDLTLKQLDQLVMALKAARFFKG
                     YEIIRQGAPGEDFYLIATGRVSVWVDKGKGRVKKAELRADDFFGEMALISNGPRNATV
                     VAEGQVEVFVLQKSDFEKILLKNPAIAEKLKKAYEARAAENRSQA"
     gene            995..>1346
                     /locus_tag="VFR02_07085"
     CDS             995..>1346
                     /locus_tag="VFR02_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9870240.1"
                     /translation="MRLKTQLALVAAVLMLAGCGPKQSSKLKIGLSLDTLKEERWQHD
                     RDFFTARAKELGAQVLVQAADNNAALQNSQAEDLLTEGVQVLVVVPHDSKSAATIVKA
                     AHQAGVPVLSYDRLI"
BASE COUNT          270 a          414 c          438 g          224 t
ORIGIN      
        1 gagctggacc tggagggcct caagaagatc gcccccaaga cccgccagat ccgcgatttc
       61 gtgaccgaat acaagttcaa gaacggcaag aagatcttcg tgctgggcga ggggcgtttg
      121 atcaacttgg ccgccgccga gggccatccg gcctcggtga tggacatgag cttcgccaac
      181 caggccctgg gcgccgaata cgtggtgaag aaccacaaca tcctggaccg caaggtctat
      241 tcggtgccgg tggagaccga ccaggagatc gcccggctca agctcaaggc cctgggcatc
      301 aagatcgaca ccctgacggc cgagcaggag cattacctgt cgtcctggga gatggggact
      361 taagcccaat aggctgtcat cgcggggaat ggccgggcgt aggcccggcc atgacgcggc
      421 aaaggcgggc ccttggggcc cgcctttttt gttcccgccg ttgagccccc cgatataatc
      481 ggggaaggca aaagccgccg tccaaagggg tccaaccgtg gttccaacct ccgccatctt
      541 cgcccacctg cggggcgtcc tgcagcgctc ctacatcatg caggacctga ccttgaaaca
      601 gctggaccag ctggtcatgg ccctgaaggc cgcccgattc ttcaagggtt acgagatcat
      661 ccgccagggc gccccggggg aggattttta cctgatcgcc accggccggg tctcggtctg
      721 ggtggacaag gggaagggcc gggtcaagaa ggccgagctg agggcggacg actttttcgg
      781 cgaaatggcg ctcatttcca acgggccgcg caacgccacc gtcgtcgccg aaggccaggt
      841 cgaggtgttc gtgctccaga agagcgactt cgagaagatc ctgctgaaga atccggccat
      901 cgccgagaag ctcaagaaag cctacgaagc ccgcgcggcg gagaaccgca gccaggctta
      961 agcccgcgcc cattctttca tccacggagg atccatgcgt ttgaagaccc agttggcgtt
     1021 ggtggcggcg gttttgatgt tggcgggctg cggcccgaag caatcctcga agctcaagat
     1081 cggcctgtcg ttggacaccc tcaaggaaga acgctggcag catgaccggg atttcttcac
     1141 ggcccgcgcc aaggaactgg gggcccaggt gctggtgcag gcggcggaca acaacgcggc
     1201 cctgcagaat tcccaggccg aggacctctt gaccgagggg gtgcaggtct tggtggtggt
     1261 gccgcacgat tccaagagcg cggccaccat cgtgaaggcc gcccaccagg ccggggtgcc
     1321 ggtgctttcc tacgaccggc tcatcc
//
LOCUS       DASTJJ010000575         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_79979, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000575 DASTJJ010000000
VERSION     DASTJJ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79979"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFR02_07090"
     CDS             <1..834
                     /locus_tag="VFR02_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9870241.1"
                     /translation="VTADPPGATYGPRLSQNYELIWIREGEVKATFGQALVLGKEGTF
                     LFRRPGVRDHYEWSKRGRTVHAYVQFKVPRGGFALPEKGVPASRPMPDNDVLRPLFNY
                     LLQLDALKEPQRTGLMVPTLELMLRSYISGQVGVKPQLALKMPELVGRSVEMIARRIA
                     QAPAKRLSLTELSKEAHTSPENLCRLFQKSLRLGPLEYAKLVRLDMAAHRLGRTHQSV
                     KEVAAATGFYDAFHLSNSFKKVYGLSPKDFKASPLNEWITQKNPIVQTLYSGPYHPPH
                     P"
BASE COUNT          205 a          376 c          341 g          205 t
ORIGIN      
        1 gtcacggccg acccgccggg cgccacctat ggcccccggc tttcccaaaa ttacgagctc
       61 atttggatca gggaagggga ggtcaaggcg accttcggcc aggccctggt cctcggcaag
      121 gaaggcacgt tccttttccg gcgcccgggg gtccgggacc attatgaatg gtccaaacgg
      181 ggccgcaccg tccatgccta tgtccaattc aaggtccccc ggggcggctt cgccttgccg
      241 gaaaaaggcg tgccggcttc ccgccctatg ccggacaacg acgtgctgcg ccccctgttc
      301 aattatttat tgcagttgga cgccttgaag gaaccccaaa ggaccggcct catggtcccg
      361 accctcgaac tgatgctccg cagctatatc tcggggcagg tgggggtgaa gccgcagctg
      421 gctttgaaga tgcccgaatt ggtggggcga tcggtggaga tgatcgcccg gcggatcgcc
      481 caggcccccg ccaagcggct gagcctgacc gagctttcca aggaagccca tacctcgccc
      541 gagaatttgt gccgtctttt ccagaagtcc ctgcggttgg gcccgctgga gtacgccaag
      601 ctggtccggc tggacatggc cgcccatcgg ctcgggcgga cccaccagag cgtcaaggag
      661 gtcgccgccg cgaccggatt ctatgacgcc ttccatttgt ccaacagctt caagaaggtc
      721 tacggcctgt cgcccaagga cttcaaggcg tcccccttga acgaatggat cacccaaaag
      781 aaccccatcg tccagaccct ctacagcggc ccctaccacc cgccccatcc ctaggccttc
      841 gggccgttga accttttccc aacccgcttc ggtcaatttt atccatccat tggtcattcc
      901 cgtacatggc cgccgggggg ggcgggaagt agacttgaaa aggcagcccg aacgggcccg
      961 cttgacggcg ggcctcaaaa ccatttcagc ggggaagaca tgaagaacgc gcttggagaa
     1021 gatccggtcc cgcccaaagc gcgggttgct ccccgggggg cgcgtcgaag cgctttcgcg
     1081 gtcctgggct tcctgctggc ggcggctttc ccggcatggg ccgggcc
//
LOCUS       DASTJJ010000576         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_690999, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000576 DASTJJ010000000
VERSION     DASTJJ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690999"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1304
                     /gene="ftsH"
                     /locus_tag="VFR02_07095"
     CDS             <1..>1304
                     /gene="ftsH"
                     /locus_tag="VFR02_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9870242.1"
                     /translation="VAGEAGVPFFSISGSDFVEMFVGVGAARVRDLFEQGKKHAPCII
                     FIDEIDAVGRQRGAGLGGGHDEREQTLNALLVEMDGFNTNSGVILIAATNRLDVLDPA
                     LLRPGRFDRQVVVDRPDLGGREAILKVHSKAVKLAEAVDLSVIARRTPGFSGADLANL
                     INEAALLAARRNKKLVEMPELEEAIDRVMAGPERRTRVISEEEKKIVAYHESGHTIVG
                     KMIDKSHTVHKVSIIPRGHAALGATWYLPNEEKFLSSKTELLNQITALLGGRTAEEIV
                     FGDVTTGAGNDLERATAIARAMICQYGMSEKLGSLTLGGKDDAVFIGRDLMKEKNYSE
                     STAQMIDAETKRIVEDCHKRAESILKKNRKFLKIMAEALLEREVLDSSEVDDILAGRK
                     IGPKPQTPAAPPAPPPPAASPVTPDIVRKPKAAPSQAHQGGGP"
BASE COUNT          245 a          453 c          433 g          173 t
ORIGIN      
        1 gtggcggggg aggcgggcgt ccccttcttc tccatctcgg gctcggactt cgtggaaatg
       61 ttcgtgggcg tgggcgcggc ccgcgtgcgc gacctctttg agcagggcaa gaaacacgcc
      121 ccctgcatca tcttcatcga cgagatcgac gccgtgggcc gccagcgcgg cgcggggctc
      181 ggcggcgggc atgacgagcg ggagcagacc ctgaacgcgc tcctggtgga aatggacggc
      241 ttcaacacca attcgggggt catcctgatc gccgccacca accggttgga cgtgctggac
      301 ccggccctgc tgcgccccgg ccgtttcgac cgccaggtgg tggtggaccg gcccgacctg
      361 ggcggccgcg aggccatcct gaaggtgcac tccaaggccg tcaaactggc cgaggcggtg
      421 gacctgtcgg tcatcgcccg ccgcacgccc ggcttctcgg gggcggacct ggccaacctg
      481 atcaacgagg cggcgctcct ggccgcccgc cgcaacaaga agctggtgga gatgcccgag
      541 ttggaggagg ccatcgaccg cgtcatggcc ggccccgagc gccgcacccg ggtcatctcg
      601 gaagaagaaa agaagatcgt ggcctaccac gagtcgggcc acaccatcgt gggcaagatg
      661 atcgacaagt cccacacggt ccacaaggtg tccatcatcc cccggggcca cgcggccttg
      721 ggcgccacct ggtacctgcc caacgaggag aagttcctgt cctccaagac cgaattgttg
      781 aaccagatca ccgcgctgtt gggcggccgc accgccgagg agatcgtctt cggcgacgtc
      841 accacgggcg ccggcaacga cctggaacgg gccacggcca tcgcccgggc catgatctgc
      901 caatacggca tgagcgagaa gctggggtcc ctcaccctgg gcggcaagga cgacgcggtc
      961 ttcatcggcc gggacctgat gaaggaaaag aactactcgg aaagcacggc ccaaatgatc
     1021 gacgcggaga ccaagcgcat cgtggaggac tgccacaagc gcgccgagtc catcctcaag
     1081 aagaaccgga agttcctcaa gatcatggcg gaagccctgc tcgaacggga agtgctggac
     1141 agctcggaag tggacgacat cctggcgggg cgcaagatcg gccccaaacc ccagaccccc
     1201 gccgcgcccc cggccccgcc gccgcccgcg gcgtccccgg tcacgcccga catcgtgcgc
     1261 aagcccaagg cggccccgtc ccaggcccac cagggtggag gccc
//
LOCUS       DASTJJ010000577         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1058943, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000577 DASTJJ010000000
VERSION     DASTJJ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058943"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1044)
                     /locus_tag="VFR02_07100"
     CDS             complement(46..1044)
                     /locus_tag="VFR02_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylhydrolase"
                     /protein_id="HET9870243.1"
                     /translation="MSRARFLTPALVLAAGLLVPPGAGAQDAVIATSDRSGVIGSLVL
                     EDPSRHRRVPLTVYYPVGQGRWPFPVIVFSHGAGGSKDGYAYLGRYWASHGYVVIHPT
                     HFGSDASLLRRHRPFYNLRAIKKMVTTPRNFIDRPQDISFVLDHLAQVEGMDPELDGL
                     FDLKRVGVAGHSFGAYTTLAVAGAVVSLKGQKPRSFRDPRPLAFIALSPQGDEPGVFE
                     GNAWAGIRRPFLVMTGTEDKGLGGQPASWRQQPYGGMPPTGNKDQIVITGADHMNFAG
                     VGLNGKVRDGAKNEYIQKATLTFWDAYLKGDLTAQRDVQSGYFPKVDGVEAAVQEK"
     gene            complement(1041..1898)
                     /gene="pgl"
                     /locus_tag="VFR02_07105"
     CDS             complement(1041..1898)
                     /gene="pgl"
                     /locus_tag="VFR02_07105"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9870244.1"
                     /translation="MPAKKKKTKRPQGKPQKPAAKARLKRAPGKAAKAAKPIGFRPEI
                     KRYADAQGLFDEAAHLFLETARQAVAARGRFTAALSGGSTPKGLFQQLAEEPYLSLMP
                     WSKTFLFWVDERHVPFTDETSNFRMTKENLLSKVPLPPENITPGTDPARPVAEAAAWL
                     EGRLKKFFGAGAPPVFDYCLMGMGDDGHTASLFPGMPQLNERDKWVVGYFVDPAKKER
                     VSLTFPVLNAARLLVVLAEGPKKADMLAKVLEGPSDPPRYPIQYLRPTAGRLLFMVDQ
                     AAAARLKKK"
     gene            complement(1933..3030)
                     /locus_tag="VFR02_07110"
     CDS             complement(1933..3030)
                     /locus_tag="VFR02_07110"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET9870245.1"
                     /translation="MSVSSQAIEHELTELWKETKPAKEGEPGLQRVYTTNLVAYAEDH
                     DTGYRVERVLMDLTQFHPGRYILIRPAQDTGEAPLRYYVSGYCPFWTDQREHRVCCDI
                     IKLVAQQDTIENLYGFTFSLLVPDLPVELWWPGDLPIKNKFFGKMAEASNRVWVDSAR
                     FKNPEMSIARLAAVWEKRFPNTLLADMNWVRIQRWRALIAELFDGPWAKFLNDIKQVT
                     IEYGEGTHSLRSFFLALWMAGRLGWVYKGGPLGLIPEKLEFEGPRGPIDVLLKPVPVQ
                     DGKCDRIFAVGVMTGGDRPGIFTVVRDADPAVVVARAEVEQQEAFSRVVTFDHLHSNQ
                     VLAAGLRHQARDDNWMAVLQLAGSILDTTSL"
     gene            complement(3027..>3706)
                     /locus_tag="VFR02_07115"
     CDS             complement(3027..>3706)
                     /locus_tag="VFR02_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9870246.1"
                     /translation="LKILQSIRPFHPESIAQFAVRGQYGPGYLAGQKVPGYREENEVD
                     PKSITPTYAALKVFLDTWRWHGVPFFLRSAKRLPKRSTEVAIQFKEPPNLLFKKNVGG
                     ISPNLLVIRIQPDEGMTLRFETKVPGMTTEARTVNMDFRYGTSFAEDTPEAYERLLLD
                     CMLGDATLFIRGDEAEAAWQALMPVLNHWENTQPEVPFPNYEAGTWGPAEADQLLERP
                     WRKWRRL"
BASE COUNT          576 a         1187 c         1303 g          640 t
ORIGIN      
        1 atcaatgttt catcggcttt gctttgtgaa gtggatgatc ccgaattatt tttcctggac
       61 ggcggcttcg accccgtcca ccttggggaa atagccgctt tggacgtccc gctgggccgt
      121 caggtcgccc ttcaggtagg cgtcccaaaa ggtcagggtg gccttttgga tgtattcgtt
      181 cttggccccg tcccgcacct tgccgttcaa ccccacgccc gcgaaattca tgtggtccgc
      241 gcccgtgatc acgatctgat ccttattgcc cgtgggcggc atgcccccat agggttgctg
      301 ccgccaagag gccggctggc cccccaggcc cttgtcctcg gtccccgtca tgaccaagaa
      361 gggtcgccgg atgcccgccc aggcgttccc ctcaaagacg cccggctcgt ccccctgggg
      421 cgacagggcg atgaaggcca gcggccgggg gtcccggaag gaccgcggct tttggccctt
      481 caaggacacc accgcgcccg ccaccgccaa cgtggtgtag gccccgaagg aatggcccgc
      541 cacccccacc cgtttgaggt cgaagaggcc gtccaattcg gggtccatgc cctccacctg
      601 cgccaaatgg tccagcacga aggaaatgtc ctgcggccgg tcgatgaaat tacggggcgt
      661 ggtcaccatc ttcttgatgg cccgcaggtt gtagaagggc cggtgcctgc gcagcaggga
      721 cgcgtcgctc ccgaaatggg tgggatggat gaccacgtag ccgtgggagg cccagtagcg
      781 gcccaggtag gcgtagccgt ccttcgagcc gccggcgccg tgcgagaaga cgatgacggg
      841 aaagggccag cggccctgcc ccacggggta atagacggtc agggggacgc gccggtgccg
      901 ggaagggtcc tccaggacca aggagccgat cacgccgctt cggtcgctgg tggcgatgac
      961 cgcgtcctgg gccccagcgc ccggggggac gagcagcccg gcggccagga cgagggccgg
     1021 cgtcaggaac cgggcccggc tcatttcttc ttgaggcggg ccgccgcggc ctggtccacc
     1081 atgaagagca gccggcccgc ggtgggccgc aggtattgga tcgggtagcg gggcgggtcc
     1141 gaggggccct ccagcacctt cgccagcatg tcggccttct tcggcccctc ggccaacacc
     1201 accagcagcc gggccgcgtt cagcacgggg aaggtcaggg acacccgttc cttcttggcc
     1261 gggtccacga aataacccac cacccatttg tcccgctcgt tcaactgggg catgccgggg
     1321 aaaaggctgg ccgtgtgccc gtcgtcgccc atgcccatca ggcaatagtc gaagaccggg
     1381 ggggcgccgg ccccgaagaa tttcttgagg cggccctcca gccaggcggc cgcctccgcc
     1441 accgggcggg cggggtccgt gccgggcgtg atgttctcgg gcggcagggg aaccttggaa
     1501 aggaggttct ccttcgtcat gcggaaattg ctggtctcat cggtgaaggg cacgtgccgc
     1561 tcgtccaccc agaacaggaa ggtcttcgac cagggcatga gggacaggta gggctcctcg
     1621 gcgagctgtt ggaaaagccc cttgggcgtg gacccgccgg aaagcgccgc cgtgaaacgg
     1681 ccccgggcgg ccaccgcctg ccgggcggtc tccaagaaca ggtgcgcggc ctcgtcgaag
     1741 agcccctggg cgtccgcgta gcgtttgatc tcggggcgga agccaatggg tttggcggct
     1801 ttcgcggcct tcccgggggc ccgcttcagc cgggccttgg ccgccggctt ttggggcttc
     1861 ccctgggggc gcttcgtctt tttctttttg gcgggcatgg cgtctcctaa agggtgcggc
     1921 tgggaaggct cgttacagcg aggtggtgtc caggatcgat ccggccaact gcaggaccgc
     1981 catccagttg tcgtcgcgcg cttggtgccg caggcccgcg gccaggacct ggttggaatg
     2041 caggtggtcg aaggtgacca cccgggaaaa ggcctcctgc tgctccacct cggcccgcgc
     2101 caccaccacg gcggggtcgg cgtcgcgcac cacggtgaag atgccgggac ggtcgccccc
     2161 cgtcatgacg cccaccgcga agatgcggtc gcacttgccg tcctgcacgg gcacgggctt
     2221 gagcaggacg tcgatgggcc cccgggggcc ctcgaattcc agtttctcgg ggatgaggcc
     2281 cagcgggcct cccttataga cccagcccag ccggccggcc atccagaggg ccaggaagaa
     2341 gctgcgcagg gaatgggtgc cctcgccgta ctcgatggtc acctgcttga tgtcgttgag
     2401 gaacttggcc cagggcccgt cgaaaagctc ggcgatgagc gcccgccagc gttggatgcg
     2461 cacccagttc atgtcggcca agagcgtgtt ggggaagcgc ttttcccaca ccgcggcgag
     2521 ccgggcgatg gacatctcgg ggttcttgaa ccgggccgaa tcgacccaga cccggttgga
     2581 ggcctcggcc atcttgccga agaacttgtt cttgatgggc aggtcccccg gccaccaaag
     2641 ctccacgggc aggtcgggca ccagcagcga gaaggtgaag ccgtagaggt tctcgatggt
     2701 gtcctgctgg gccaccagtt tgatgatgtc gcagcagacc cggtgctcgc gctggtcggt
     2761 ccagaagggg caatagcccg acacgtaata gcgaagcggc gcctcgccgg tgtcctgcgc
     2821 cggccggatg aggatgtagc ggcccggatg gaactgggtg aggtccatca ggacccgttc
     2881 cacccggtag cccgtgtcgt ggtcctcggc gtaggccacc aggttggtgg tgtagacgcg
     2941 ctgcaggccc ggctcgcctt ccttggcggg cttggtctct ttccagagct cggtgagctc
     3001 gtgctcgatg gcctgggaag agacgctcac aggcgcctcc atttgcgcca ggggcgctcc
     3061 agcagctggt cggcttccgc cgggccccag gtgccggcct cgtagttggg gaaaggcacc
     3121 tcgggctggg tgttctccca gtggttcagc accggcatga gggcctgcca ggcggcctcg
     3181 gcctcgtcgc cgcggatgaa gagggtggcg tcgcccagca tgcagtcgag cagaaggcgc
     3241 tcgtaggcct ccggggtgtc ctcggcgaag ctggtgccgt agcggaagtc catgttgacc
     3301 gtgcgcgcct cggtggtcat gcccggcacc ttggtctcga agcgcagcgt catgccctcg
     3361 tcgggctgga tgcggatgac caacaggttg ggcgagatgc cccctacgtt cttcttgaag
     3421 agcaggttgg ggggttcctt gaactggatg gccacctcgg tggagcgctt gggcaggcgc
     3481 ttggccgagc gcaggaaaaa gggcacgccg tgccagcgcc aggtgtcgag gaagaccttc
     3541 agggccgcgt aggtgggcgt gatggacttg gggtccacct cgttctcctc gcggtagccc
     3601 ggcaccttct ggcccgccag gtagcccggg ccgtactggc cccgtacggc gaactgggcg
     3661 atggactcgg ggtggaaggg gcggatggac tgcaggatct tcagct
//
LOCUS       DASTJJ010000578         1264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_325646, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000578 DASTJJ010000000
VERSION     DASTJJ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325646"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFR02_07120"
     CDS             complement(<1..541)
                     /locus_tag="VFR02_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9870247.1"
                     /translation="MAIPRFVARPDWYTSANPETLAATGTPLRWGVVATGNIATTVTG
                     DLALLEDAVLQAVSSRREAGAAAFAEKFGFATHYFDGGHDGPGAPGHLQLVQDPDVDV
                     VYIATPHAQHYDIARAALEHGKHVLCEKSLTITAAEARSLVELARSRQLFLMEAVWAR
                     FVPGFQRALEIVAAGEIGEV"
     gene            complement(659..>1264)
                     /locus_tag="VFR02_07125"
     CDS             complement(659..>1264)
                     /locus_tag="VFR02_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9870248.1"
                     /translation="KAKKPNGPYNIELFAGSPDDANSKVFFDGAMKVLQPKIDDGTLK
                     VVSGQKTFPQAVTQGWKAENAQKRMDSLLVGSYSSASLDGVLSPNDTLARAIITSVKA
                     AGKPIPTVTGQDSEVQSVKSIMAGEQYSTINKDTTALVNHAIDMVKGLQKGTKPEIND
                     PTSYNNGVKVVPAYLLVPKIVTKANAAEQYANDKVLGPLTK"
BASE COUNT          189 a          380 c          454 g          241 t
ORIGIN      
        1 tcacctcgcc gatctcgccg gcggccacaa tctccagcgc ccgctggaat cccgggacaa
       61 accgcgccca cacggcttcc atgaggaaca gctgccggga acgggccagt tcaaccaggg
      121 agcgggcttc ggccgccgtg atggtgaggg acttttcgca caagacgtgc ttgccatgtt
      181 ccagggcggc gcgggcgatg tcgtagtgct gggcatgcgg ggtcgcgatg tacaccacgt
      241 ccacgtccgg gtcctgcacc agctggaggt ggcccggggc acccggtccg tcgtggccgc
      301 cgtcgaaata gtgggtggca aagccgaact tttcggcaaa ggccgccgca cccgcctccc
      361 ggcgcgagct cacggcctgc agcacggcgt cctcaagcag ggccaggtcc cccgtgacgg
      421 tggtggcaat gttgccggtg gccaccacgc cccagcgcag cggggttccc gtcgccgcga
      481 gtgtttccgg gttggccgag gtgtaccagt cggggcgggc aacaaagcgg ggaatggcca
      541 tggcgccatt ctgccagttg ccgcgggcgt gggggaacac ggcaagggcc ccgtccggcg
      601 cgtcgtgatg gacgacggcg gacggggccc ttgcggcgtt ggtgccttgg gtgccgggct
      661 acttggtcag cgggcccagg accttgtcat tggcgtactg ctcggcggcg ttggccttgg
      721 tgacaatctt gggaaccagc aggtatgccg ggacaacctt gactccgttg ttgtaggagg
      781 tcgggtcgtt gatctccggc ttggtgccct tctgcaggcc cttgaccatg tcgatggcgt
      841 ggttgaccag ggccgtggtg tccttgttga tcgtggagta ctgctccccg gccatgatgg
      901 acttgacgga ctgaacctcg gaatcctggc cggtgacggt cgggatgggc ttgccggcag
      961 ccttcacgga cgtgatgatg gcccgggcca gggtgtcgtt cggggagagg accccgtcca
     1021 gggaagcgct gctgtagctg ccgaccagaa gggagtccat gcgcttctgc gcgttttcag
     1081 ccttccatcc ctgggtcacg gcctgcggga acgtcttctg gccggagaca accttcaggg
     1141 tgccgtcgtc gatcttgggt tggaggacct tcatggcgcc gtcaaagaag accttggagt
     1201 tggcatcatc gggggagccg gcaaagagct caatgttgta cgggccgttg ggcttcttgg
     1261 cctt
//
LOCUS       DASTJJ010000579         1795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_80749, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000579 DASTJJ010000000
VERSION     DASTJJ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80749"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1795
                     /gene="ligA"
                     /locus_tag="VFR02_07130"
     CDS             <1..>1795
                     /gene="ligA"
                     /locus_tag="VFR02_07130"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9870249.1"
                     /translation="TSKHFKTRPHRVPMLSLDNTYTLEELREFDERVKKGLGGEKAEY
                     VCELKIDGVSIELVYEKGALTQALTRGDGEKGDDVLANVRNIPGLPHQLKGPGLPERL
                     EIRGEVFFTRKDFEAVNEDRREKGLELFANPRNTASGTLKTVDPKESAKKPLRILLYY
                     VLSPGKLPFATQKESLQWLAKMGFPEDKHFRLCRDLAAVQAYCEEYQAKRDKIGFDCD
                     GVVLKVDRFDQREKLGETTKIPRWAVAFKFAAQKAETTLKAITLQVGRTGAVTPVAEL
                     EPVLLEGTTVSRATLHNEDEIKRKDLRVGDRVLVEKGGLVIPKVLKSFPEKRKGGEKP
                     FQMPSACPVCASPLEREEDEAAWRCENPACPAQVEGRIEHFCGRDAMDIRGAGPAVVK
                     QLLEEKLIRDYADLYALKEKDVAELERKAEKSAQNLMGQIEESRRRTLGRLLFGLGIR
                     HVGVHVAEILAARHADLWALGEASEEALTAIDGIGPVVAHSIVAFFSQAGNVKILKKL
                     AKNGVNLKRLKEEEPSGEQPFADQTFVFTGELKAYGRKEAEDLVKKLGAKAAGSVSKL
                     TTYVVAGEAAGSKLKKAKELGVTVMTEAEFEK"
BASE COUNT          357 a          567 c          622 g          249 t
ORIGIN      
        1 caccagcaag cacttcaaga cccggccgca ccgggtgccc atgctgtccc tggacaacac
       61 ctacaccctg gaggaactga gggagttcga cgagcgggtg aagaagggcc tggggggcga
      121 aaaggccgag tatgtgtgcg agctcaagat cgacggggtc agcatcgagt tggtctacga
      181 gaagggggcc ctgacccagg ccctcacccg gggcgacggg gaaaagggcg acgacgtgct
      241 ggcgaacgtc cgcaacatcc cgggcctgcc ccatcagctc aaggggccgg gcctgcccga
      301 gcggctggag atccggggcg aggtcttctt cacccgcaag gacttcgagg cggtcaacga
      361 ggaccggcgg gaaaagggcc tggagctttt cgccaacccc cgcaacaccg cctcgggcac
      421 cctgaagacc gtcgacccca aggaatccgc caagaagccc ctgcgcatcc tcctttatta
      481 cgtcctgtcc ccgggcaagc tgcccttcgc cacccagaag gagagcctgc aatggctggc
      541 caagatgggt tttcccgagg acaagcattt ccgcctctgc cgggacctgg cggcggtcca
      601 ggcctattgt gaggaatacc aggccaagcg cgacaagatc ggcttcgact gcgacggcgt
      661 ggtgttgaag gtcgaccgct tcgaccagcg ggaaaaattg ggcgagacca ccaagatccc
      721 ccggtgggcc gtggccttca agttcgccgc ccagaaggcc gagaccaccc tgaaggccat
      781 cacgctccag gtgggccgca cgggcgccgt cacgccggtg gccgagttgg agcccgtcct
      841 gctcgagggc accaccgtgt cccgggccac cctgcacaac gaggacgaga tcaagcgcaa
      901 ggacctgcgg gtgggggacc gggtgctggt ggagaagggc gggctggtca tccccaaggt
      961 gctgaagtcc ttcccggaaa agcgcaaggg gggcgagaaa cccttccaga tgccctcggc
     1021 ctgcccggtc tgcgcctccc ccctggagcg cgaggaggac gaggcggcct ggcgctgcga
     1081 gaacccggcc tgcccggccc aggtggaggg ccgcatcgag catttttgcg gccgcgacgc
     1141 catggacatc cggggcgcgg gccccgcggt ggtgaagcag ctgttggagg aaaagctcat
     1201 ccgggactac gccgaccttt acgccctcaa ggaaaaggac gtggccgagc tggagcgcaa
     1261 ggccgagaag tccgcccaga acctcatggg ccagatcgag gagagccgcc ggcgcaccct
     1321 gggacggctg ctcttcggcc tggggatccg gcacgtgggc gtgcacgtgg ccgagatcct
     1381 ggccgcgcgg cacgcggacc tctgggccct gggggaggcc tccgaggagg cgctgacggc
     1441 catcgacggc atcgggcccg tggtggccca ttcgatcgtg gccttctttt cccaggcggg
     1501 gaacgtcaag atcctcaaga aactcgcgaa gaacggggtc aacctcaagc gtttaaagga
     1561 ggaagaaccc tccggggagc aacccttcgc ggaccagacc ttcgtcttta cgggtgagct
     1621 gaaggcctac ggccgcaagg aggccgagga cctggtgaag aaactgggcg ccaaggcggc
     1681 gggttcggtg tccaagctca ccacctacgt ggtggccggc gaggcggcgg gttccaagct
     1741 caagaaagcc aaggaattgg gcgtgacggt catgaccgaa gccgaattcg aaaaa
//
LOCUS       DASTJJ010000580         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_447642, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000580 DASTJJ010000000
VERSION     DASTJJ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447642"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFR02_07135"
     CDS             complement(<1..598)
                     /locus_tag="VFR02_07135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET9870250.1"
                     /translation="MNLEASDLRRAFEGQALASFFPASQEESLARFTRRMLELNQGLN
                     LTKWTQPEEALVHHVLDSAFGIPLAAGFSPAGEWMDLGTGGGFPGALMVAAFPKVPFT
                     FVDAVAKKGRAVADCLEAAGWKARVLTERAEVLGRDPAYRDRFEGVVARAVADLPVLL
                     EYALPFLKPQGHLLSWMTQEQVQKVDKAKKAIDELGARI"
     gene            complement(595..2481)
                     /gene="mnmG"
                     /locus_tag="VFR02_07140"
     CDS             complement(595..2481)
                     /gene="mnmG"
                     /locus_tag="VFR02_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET9870251.1"
                     /translation="MAFPSSSTFDVIVVGAGHAGCEASLACARMGLTTALLTMDPDTV
                     AKMSCNPAVGGLAKGHLVREIDALGGEMAKACDATSIQSKILNRTQGPAVWGFRAQCD
                     KELYNQRMRAVVFGQENLTVKQALVERLLVEGGRAAGVLTQYGETVRARAVVVTTGTF
                     LNALMHIGETKIEGGRTGELSAKGLPAQLRELGFTVGRLKTGTNPRVHRDSIDFSKTE
                     EQWGDADPMPFSHFTTQFPLQPQVACHITYTNDATHQAIRDNMARSPLYSGVIKGIGP
                     RYCPSIEDKVVKFPDKGRHQIFLEPEGLASNEYYLNGLSTSLPLDAQYAFLRTIPGLE
                     GAQIVRPGYAVEYDFVPPTQVRHTLETYRLPGLFLAGQINGTSGYEEAAAQGLMAGLN
                     AALSVKGQPGFVLSRTEAYIGVLIDDLVTKGTEEPYRLFTSKAEYRLLLRPDNADLRL
                     MEKGHELGLIPRPAYEGVVKKKAKIEAGLRAFKSRTLPPAAAPNEALKLTLSDRAVKL
                     EDVLRRPWITYEALKPYLPEDLQWDPEVSFQVEVELKYEGYLRKQRLEIERQNRYEGF
                     AIPRDFPYLATGGFSREAKEKLDRIRPETFGQASRIQGVSPADLSVLLLALSRFSKSR
                     LSSL"
BASE COUNT          464 a          831 c          893 g          468 t
ORIGIN      
        1 cgatccgcgc gcctaattcg tcgatggctt tttttgcctt atccactttt tggacctgct
       61 cctgggtcat ccagctcagc aggtgtcctt ggggcttcag gaagggaagg gcatattcca
      121 ggagcacggg taggtcggcc accgcccggg ccaccacgcc ctcgaaccgg tcccggtagg
      181 cggggtcgcg ccctaagacc tcggcccgct ccgtgaggac ccgcgccttc cagcccgcgg
      241 cttccaggca atccgccacc gcgcggccct tcttggccac ggcgtccacg aaggtgaagg
      301 ggaccttcgg aaaggccgcc accatcaagg cgcccgggaa cccgccgcct gtgcccaggt
      361 ccatccattc ccccgcgggc gagaaccccg cggccagcgg gatcccgaag gcggagtcca
      421 gcacatgatg caccaaagct tcctcgggct gggtccattt ggtgaggttc aggccttggt
      481 tgagctccag catgcggcgg gtgaagcggg ccagggattc ctcttgggag gcggggaaga
      541 aggacgcgag ggcctgccct tcgaacgccc ggcgcaggtc ggaggcttcg agattcaaag
      601 ggacgagagc cggctcttgg aaaagcggct cagggccaag agcagcacgc tcaaatccgc
      661 cggggacacc ccctggatgc gggaagcctg gccgaaggtc tcgggccgga tgcggtcgag
      721 cttttccttg gcctcccggg agaagccccc ggtcgccaga taggggaaat cccgggggat
      781 ggcaaagccc tcatagcggt tctggcgctc gatctccagg cgttgtttgc gcaaatagcc
      841 ttcgtacttg agctccacct ccacctggaa gctgacctcc gggtcccatt gcaggtcctc
      901 cggcaggtag ggtttcaggg cctcataggt gatccagggg cgccgcaaga catcctccag
      961 cttcacggca cggtccgaaa gggtcagttt cagggcctcg ttgggggcgg ccgccggggg
     1021 cagcgtccgg ctcttgaagg ccctcaatcc cgcctcgatc ttcgcctttt ttttcaccac
     1081 cccttcatag gccggcctgg gaatgagtcc cagttcatgc cccttttcca tcagccgcag
     1141 gtccgcgttg tccggccgca gcagcaggcg gtattcggcc ttggaggtga agagccggta
     1201 gggttcctcg gtgcccttgg tgaccaggtc gtcgatcaag acgccgatat aggcctcggt
     1261 gcgcgaaagc acgaagccgg gctgtccctt caccgacaag gcggcgttca agccggccat
     1321 caggccctgg gccgcggcct cctcatagcc ggaagtgccg ttgatctggc cggccaggaa
     1381 caggcccggc aggcggtagg tctccagcgt atggcgcacc tgggtgggcg gcacgaaatc
     1441 atattccacc gcgtagcccg gccggacgat ctgggcccct tccaaccccg ggatggtgcg
     1501 caggaaggcg tattgggcgt ccaggggcag ggaggtggag aggccgttga ggtaatactc
     1561 gttgctggcc aacccctcgg gctccaggaa gatctggtgg cggcccttgt cggggaactt
     1621 caccaccttg tcctcgatgg aggggcaata gcgggggccg atgcccttga tgacgcccga
     1681 ataaagcggg gaccgggcca tgttgtcccg gatggcctgg tgggtggcgt cgttggtgta
     1741 ggtgatatgg caggccacct ggggttgcag ggggaattgg gtggtgaaat gcgagaaggg
     1801 catcgggtcg gcgtcgcccc actgttcctc ggtcttggaa aaatcgatgg agtcgcggtg
     1861 gacccgcggg ttggtgccgg tcttcaggcg gcccacggtg aaacccagct cgcgcaattg
     1921 ggccggcaag cccttggccg aaagttcacc cgtacgcccg ccctcgatct tcgtctcgcc
     1981 gatgtgcatg agggcgttga ggaaggtgcc cgtggtcacc accacggccc tcgcgcggac
     2041 cgtctcgcca tattgggtga ggacgccggc cgcgcggccg ccttcgacca acaggcgctc
     2101 gaccagggcc tgcttgaccg tgaggttctc ctggccgaag accaccgcgc gcatgcgctg
     2161 gttgtagagc tccttatcac actgggcccg gaagccccag accgcgggac cctgggtccg
     2221 gttgaggatc ttggactgga tggacgtggc gtcgcaggcc ttcgccatct cgccgccgag
     2281 ggcgtcgatc tcgcgcacca ggtggccctt ggccaaaccg cccaccgccg gattgcagct
     2341 catcttggcc acggtgtccg ggtccatcgt gagcagggcg gtggtgaggc ccatgcgggc
     2401 gcaggcgagc gaagcctcgc aaccggcatg gccggcgccg accacgatca cgtcaaaggt
     2461 tgaagaagag ggaaaggcca cccgcgaacc gccccgcgaa aataagaacc gaggggcaac
     2521 ttatagcata agaaaggaag ccgcacaatg cgggcgggaa agccccggat gttccacgtg
     2581 gaacatccgg aacgaaggcc gggtggcggg attaatgact ttttttgttc atttataaca
     2641 tcatttcccg atgcaa
//
LOCUS       DASTJJ010000581         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_813415, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000581 DASTJJ010000000
VERSION     DASTJJ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813415"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFR02_07145"
     CDS             complement(<1..1355)
                     /locus_tag="VFR02_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9870252.1"
                     /translation="MPFNLKSDFKPTGDQPRAIQQMVQHLEAGAPAQTLMGVTGSGKT
                     FSMAHVIQQSQRPALIISHNKTLAAQLYSEFKEFFPDNAVAYFVSYYDYYQPEAYLPH
                     TDTFIEKDASINMELDRLRLEATALLQERRDVIIVASVSCIYGLGSPENYKKMHLVLT
                     EGTEVHREGFLRDLVDIQYARNDIDFTRGKFRVKGDVIEVYPAYEENPFRIEFFGDEI
                     DKISEIDPVTGKALNRRKVLPIYPAKHYVMPEEVKKGALDAIREELRLRLVELKAANK
                     LVEAQRLEQRTTYDLEMIEEMGYCQGIENYSRHFEDRQAGDPPYTLLDYFPKDFITFI
                     DESHVTLPQLRGMFNGDFARKKNLVDFGFRLKCAYDNRPLRFEEFGKKVGQVVYVSAT
                     PSAEDLKKSPDRVVEQIIRPTGLMDPEIEVRPAKTQVDDLLGEARERAGKGQRTLVTT
                     LT"
     gene            complement(1466..1876)
                     /locus_tag="VFR02_07150"
     CDS             complement(1466..1876)
                     /locus_tag="VFR02_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870253.1"
                     /translation="MPMVRSAGVTIGNGDTATPTPSPTPTVTMTPSPTPTAVPEDEVS
                     VVAEPNLSNGGQPIRFEVELGQAAQVHLILYTLAGEKVYEMKAAGTAGINDLDWSLRN
                     TANAGVASGLYLYAVEVTNRNLNYVKKGQVVVLH"
BASE COUNT          354 a          664 c          663 g          414 t
ORIGIN      
        1 gtcagggtgg tcaccagggt gcgctggccc ttccccgccc gctcccgggc ctcgcccaac
       61 aggtcgtcca cctgggtctt ggccggccgg acctcgatct cggggtccat gagccccgtg
      121 gggcggatga tctgctccac cacccggtcc gggctcttct tcaggtcctc ggcgctgggc
      181 gtggccgaca cgtagaccac ctgccccacc ttcttgccga attcctcgaa acgcaggggc
      241 cggttgtcgt aggcgcactt gagccggaag ccgaaatcca ccaggttctt cttgcgcgcg
      301 aagtccccgt tgaacatgcc ccgcagctgg gggagggtca cgtgggactc gtcgatgaag
      361 gtgatgaagt ccttggggaa ataatccagc agggtgtagg gcgggtcgcc ggcctggcgg
      421 tcctcgaaat ggcggctgta gttctcgatg ccttggcagt agcccatctc ctcgatcatc
      481 tccaggtcgt aggtggtgcg ctgttccagg cgctgggcct ccaccagctt gttggcggcc
      541 ttgagctcca ccaagcgcag gcgcagttcc tcccggatgg cgtccagcgc gcccttcttg
      601 acctcctcgg gcatgacata gtgcttggcc gggtagatgg gcagcacctt gcgccggttg
      661 agggccttcc ccgtcaccgg gtcgatctcc gagatcttgt cgatctcatc cccgaagaac
      721 tcgatccgga aggggttctc ctcataggcc gggtagacct cgatgacgtc ccccttgacg
      781 cggaacttgc cccgggtgaa gtcgatgtcg ttgcgggcgt actggatgtc caccaggtcc
      841 cgcaggaatc cctcccggtg cacctccgtg ccctcggtga gcaccaggtg catcttcttg
      901 tagttctcgg gcgagcccag gccgtagatg caggacaccg aggccacgat gatgacgtcc
      961 cgccgctcct gcagcagggc ggtggcctcc aaccggagcc ggtccagctc catgttgatg
     1021 gaggcgtcct tctcgatgaa ggtgtcggta tggggcaggt aggcctcggg ctggtaatag
     1081 tcgtaatagc tcacgaaata ggccaccgcg ttgtccggga agaactcctt gaactcgctg
     1141 tagagctggg cggccagggt cttgttgtgg gagatgatga gcgccgggcg ctggctctgc
     1201 tggatgacgt gggccatgga gaaggtcttg cccgagcccg tgacccccat cagggtctgg
     1261 gcgggggccc ccgcttccag gtgttggacc atctgctgga tggcccgggg ctggtcgccc
     1321 gtgggcttga aatcggactt caggttgaag ggcatgggtt gattttggga taggaagggg
     1381 ccgaaagcaa ggcccgcgtg acggcggtcc cccaacaaaa aagcccgccc cggctttcgc
     1441 cggagcgggc ttgggaggcc ctgttttaat gcaggacgac cacttggccc ttcttcacgt
     1501 agttcaggtt ccgattcgtc acttccaccg cgtaaagata aagcccgctg gcaacgcccg
     1561 cattggccgt attccgcaag ctccaatcca ggtcgttgat cccagccgtt ccggccgcct
     1621 tcatttcgta gaccttctcc cccgccaggg tgtaaaggat cagatggacc tgggcggcct
     1681 gccccagttc gacctcgaac cggatcggtt gtcccccgtt cgaaaggttc ggctccgcga
     1741 cgacggaaac ctcatcctct ggaacagccg tcggggtcgg cgacggggtc atcgtcaccg
     1801 tcggggtggg cgacggcgtc ggcgtggccg tgtccccatt tccgatggtg acacccgccg
     1861 accgaaccat cggcatgggc cctcccggcg taggcgtcgg caacaaacaa caaccggaat
     1921 tccacagcca tgtggtccca gacgacgcgg gcgcccaaac cttatcctca tacgagatcg
     1981 gggtcacgcc ccccattccg ctgaatggag ttgaactatt ggtctgggcc caattgatgc
     2041 cgtcctgaga aacccaaacg ctggaaccag aaagtatcca gatcgcattg ccaca
//
LOCUS       DASTJJ010000582         1244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_813537, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000582 DASTJJ010000000
VERSION     DASTJJ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813537"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFR02_07155"
     CDS             complement(<1..329)
                     /locus_tag="VFR02_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870254.1"
                     /translation="MTGRGMEPEDEPYFSKILDLVLRVKGFDGQHYKPNYVKRRVAVR
                     MRATHSGSYRDYFHFLSQHPEEATQLLDRLTIHVTEFFRDAVMYGALRERVIPDLFAG
                     TADGRVKA"
     gene            complement(367..1218)
                     /locus_tag="VFR02_07160"
     CDS             complement(367..1218)
                     /locus_tag="VFR02_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870255.1"
                     /translation="MFADRVKAGRWGMMTALWLGLAAGAWAAAVETDRTAAAPGAAAP
                     DEAAASPMPRVRISDNVGFYYFVSAGYVAPDPERIPRLGQVAGDFSYRMIFGTPQKVW
                     VDLHNALVRPGDFLVVYRLGESLDGGPAGDLGRRVENLAILEVQQLDKGRCLTVVKES
                     FAPFEGGDPVKSYGDEVVRWKQAQRRKVLPDKTLTGRVAAGPVGKELFSQGDWLVLSL
                     GQKDGVVEGQSFDLVRREKEGITDPEAHKPVGKAQVLYVGGGYCMAQIVRSYEPVRVG
                     DEARYRP"
BASE COUNT          221 a          465 c          353 g          205 t
ORIGIN      
        1 gccttcaccc gtccgtcggc cgtccccgcg aacaggtccg ggatcacccg ctcccgcagg
       61 gccccataca tcaccgcatc ccggaagaac tcggtcacgt ggatggtcaa gcggtccaac
      121 aattgggtgg cctcttccgg atgctgggaa aggaaatgga agtaatcccg atacgacccg
      181 gagtgggtcg cccgcatgcg caccgcgacc cgacgcttca cgtagttcgg cttgtaatgc
      241 tggccgtcga agcccttgac ccgcaacacc aggtccagga tctttgaaaa ataaggttcg
      301 tcctccggct ccatgccccg acccgtcatt tcccggcccc tgggcgccgt tcacgtcgtg
      361 gagggttcag ggcctgtaac gggcctcgtc ccccacccgg accggctcat aggaccggac
      421 gatctgcgcc atgcaatagc ccccgcccac atagaggacc tgggctttcc ccacgggctt
      481 atgggcttcc gggtccgtga tcccctcttt ctcgcgccgg accaggtcga aggactgccc
      541 ctccaccacg ccgtcctttt gccccaagga cagcaccagc caatcaccct gggaaaaaag
      601 ctctttgccc accggaccgg cggccacccg gcccgtcagg gtcttgtcgg gcaggacctt
      661 gcggcgttgc gcctgtttcc agcggacgac ctcatcgccg taggacttca ccggatcgcc
      721 gccctcaaag ggggcgaagg actccttgac caccgtcagg caacggcctt tatccagttg
      781 ctggacctcc aagatggcca ggttctccac ccgccttccc aggtcgcccg ccggtccgcc
      841 gtccaaggac tcccccagac ggtacaccac caggaaatcc ccgggccgca ccagggcgtt
      901 gtgcaggtcg acccaaacct tttgcggcgt gccgaagatc atccggtatg aaaaatcgcc
      961 cgccacctgg ccgagccgcg ggatgcgctc cgggtccggg gccacgtaac ccgcggaaac
     1021 gaaatagtag aaacccacgt tgtccgaaat gcggaccctc ggcatggggg aagccgccgc
     1081 ctcgtccggc gccgccgcgc ccggggcggc cgccgtccgg tcggtctcca cggccgccgc
     1141 ccaggctccc gccgccaggc caagccacaa ggccgtcatc atcccccacc gtccagcctt
     1201 cacgcggtca gcgaacatgg ttcacctcgt cggaaagttc tttg
//
LOCUS       DASTJJ010000583         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_813633, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000583 DASTJJ010000000
VERSION     DASTJJ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813633"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFR02_07165"
     CDS             complement(<1..822)
                     /locus_tag="VFR02_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870256.1"
                     /translation="MTLFFLAAFPVLSFAVTAEDYYNAGNQLYQQGKLDQALQYYQAA
                     TQLDPNDWQAYQLIGYCYYTQKNNPLALQAMDKSLQINPNNPSLKQFADQLRASTPGA
                     PAPATSTSSPSAAKPAAVTDPLFLRLGLGASLPMPSQEQVQYTLGPGGVVELGYTFQP
                     GLAVFLGDDIGIWSAGNSSASITAMDISITADLQLDLLDKDGMKLYVLGGAGMDLLSF
                     VNSSSSGATASETDPAIHLGGGAAFNIGKGLDAFAQIKYGMLFTTEGSYSGTFTYI"
     gene            complement(975..1499)
                     /locus_tag="VFR02_07170"
     CDS             complement(975..1499)
                     /locus_tag="VFR02_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HET9870257.1"
                     /translation="MAEGLEAGSVERFGGAVNALAVPARPDYNPRVDRLRPSFALAAF
                     FFFFAGCSPKDLARDPALLWGVFTGVTPCADCPGIRTELSLYHSGPFVDEGAYELELT
                     YLGRNVKPFVTRGDWTMIKGDAADENASVVQLDPDHPEKSTYYLRIGPDELRQLDRDL
                     GEIDSQLNFTLKKR"
     gene            1510..2298
                     /locus_tag="VFR02_07175"
     CDS             1510..2298
                     /locus_tag="VFR02_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9870258.1"
                     /translation="MPLFELQNLSFRYPEGAFGLESLDLKVEPGGRVGLLGPNGAGKS
                     TLLRLMAGLLRPAGGRVLLDGRPLDSIPVRDRARRVAFLPQSVQFHFPLSVLEIVRMG
                     RHPYLGRFEPFSARDRGICDRALKLCDALDFRNRLFGELSGGEKQRVLLASALAQTPQ
                     VLLLDEPTLSLDWAHQLMFFDILLKLHREEGLGLVVATHEINLAGRYLGRILLMRDGR
                     VLADGPPKKAITPKLLRRSHGLEVRVLPVKGEFPYFAPKKAVRP"
     gene            2295..>3245
                     /locus_tag="VFR02_07180"
     CDS             2295..>3245
                     /locus_tag="VFR02_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9870259.1"
                     /translation="MREGSKGAVLLFSFGLFAAAWVACPLMGPTGLSYHQVFAAWPPA
                     PDNQDAQIFFSVRVPRNLLAALAGSAMAVAGVVFQSVLRNPLACPFTLGVAGGSSFGA
                     VAAIALGATASLAGFSVTTLFAFLGALLSIFLVYSLASAIRSYSAATLLLAGIGINYF
                     FNALVLLFYYLADFTQSYAMLHWMMGSLDVVEMGTVFRLALLEAVLFAFLFALGRDLG
                     LLAAGEDLAQAKGVPPKRTILASVMAASLLTSAVVAVTGPIAFVGLIVPHLYRLFTGN
                     DHRLLLASSALGGAAFLMVCDSAARSFFGPTELPVGVLTAV"
BASE COUNT          499 a          986 c         1106 g          654 t
ORIGIN      
        1 gatgtaagtg aaggtgcctg agtaagaacc ttcggtcgtg aaaagcatgc cgtatttgat
       61 ctgggcgaag gcatccagcc ccttcccgat gttgaaggcc gccccgccgc ccaggtggat
      121 ggccgggtcg gtctcgctgg cggtcgcccc gctgctggag gaattcacga aactgagcag
      181 gtccattccc gcgccgccca ggacatagag cttcatgccg tctttgtcca gcaggtcgag
      241 ttggaggtcg gcggtgatgc tgatgtccat ggccgtgatg gaggccgacg aattgcccgc
      301 cgaccagatc ccgatgtcgt cccccaggaa gaccgccagg ccgggttgga aggtgtaacc
      361 caattccacc acgccgcccg gccccagggt gtactgcacc tgttcctggg aaggcatggg
      421 cagggaggcg ccgagcccca agcgcaggaa aagcggatcg gtgacggcgg cgggtttcgc
      481 ggccgacggg gacgaggtgg aagtggccgg ggccggggcg ccgggggtgg aggcgcgcaa
      541 ttggtcggcg aattgcttca aggacgggtt gttggggttg atctggaggc tcttgtccat
      601 ggcctgcagg gccaggggat tgttcttctg ggtgtagtag caatagccga tgagttggta
      661 ggcttgccag tcgttggggt ccagttgggt ggcggcttgg taatattgaa gggcctggtc
      721 caatttgccc tgttggtaga gttggttgcc ggcgttgtaa tagtcttcgg cggtgacggc
      781 gaaggaaagg acggggaagg cggccaggaa aaaaagggtc aaaaacgatc ttttcattgt
      841 tgcctccaaa aatattgaag agggtttttc ggttaccttg gaattataag cccgttcccg
      901 ggccgaaggc caatactttg atccaaggcc gtggaagcaa aaaaggcccg tctggtgggc
      961 ggagtttgga ggcgtcagcg ttttttcaat gtgaaattga gttgggagtc gatctcccct
     1021 aagtcccggt ccaattggcg gagttcatcg ggaccgatcc gcagataata agtggacttt
     1081 tccggatggt cggggtcgag ttgcaccacg gatgcgttct cgtccgccgc gtctcccttg
     1141 atcatggtcc aatccccccg ggtgacgaac ggttttacgt tgcggcccag gtaggtcagt
     1201 tcgagttcat aggccccctc gtccacgaag gggcccgaat gataaaggga caattcggtc
     1261 cggatgcccg ggcagtcggc gcaaggcgtt accccggtga agacccccca caagagggca
     1321 gggtcccgcg cgaggtcttt cggagagcag cccgcgaaaa aaaagaagaa ggcggccaag
     1381 gcgaatgagg gtcttaagcg gtccacgcgg ggattgtagt ccggacgggc cgggaccgcc
     1441 agggcgttga cggccccccc aaacctttct acactgcctg cctcaagtcc ttctgccaaa
     1501 gagcggtcct tgcccctgtt cgaactccaa aacctatcct tccgatatcc cgagggggct
     1561 ttcgggctgg agtccctcga cctgaaggtg gagccggggg gacgggtggg tttgttgggg
     1621 cccaacggcg cggggaaatc caccctgctg cggctgatgg cggggctcct gcggcccgcg
     1681 ggcggccgcg tgcttttgga cggcaggccc ctggattcca tcccggtgcg ggaccgggcc
     1741 cggcgcgtgg ccttcctgcc ccaatccgtg cagttccatt ttcccctgtc ggtgctggag
     1801 atcgtgcgca tgggccgcca tccctacctg gggaggttcg aacccttcag cgcgcgggac
     1861 cgagggatct gcgaccgggc cctgaagctt tgtgacgccc tggactttcg gaaccgcctt
     1921 ttcggggaat tgtcgggcgg cgagaaacaa cgggtgctgc tggcctcggc cttggcccag
     1981 accccccagg tgctgctctt ggacgaaccc accctgtcgt tggactgggc ccaccagctg
     2041 atgttcttcg acatcctgct caagctgcac cgcgaggagg gcctgggcct ggtggtggcc
     2101 acccatgaga tcaacctggc ggggcgctac ctgggacgca tcctgctcat gcgggacggg
     2161 cgggtgctcg ccgacgggcc ccccaagaag gccatcacgc ccaagctctt gcggcgctcc
     2221 cacggcctgg aggtccgggt cctgcccgtg aagggcgagt tcccctattt cgcgcccaag
     2281 aaggcggtgc gtccatgagg gaaggctcca aaggggcggt cctgctcttt tcgttcggcc
     2341 tgttcgcggc ggcctgggtg gcctgtcccc tgatgggccc cacgggcctt tcctaccacc
     2401 aggtcttcgc cgcctggccg cccgcgcccg acaaccagga cgcccagatc tttttctcgg
     2461 tccgcgtccc ccggaacctt ttggcggcgc tggcgggatc ggccatggcg gtggcggggg
     2521 tggtgttcca atccgtgctg cgcaaccccc tggcctgtcc cttcaccctg ggggtggcgg
     2581 gcggcagctc cttcggggcg gtggcggcca tcgccttggg cgccaccgcc agcctggcgg
     2641 gcttctcggt gacgaccctc ttcgccttcc tgggggcgct gctttccatc ttcctggtct
     2701 attcgctggc ctcggccatc cggtcctatt cggccgccac cctgctcctg gccgggatcg
     2761 gcatcaatta tttcttcaac gccctggtgc tgctttttta ctacctggcg gacttcaccc
     2821 aaagctacgc catgctgcat tggatgatgg gcagcctgga cgtggtggag atgggcacgg
     2881 tcttccggtt ggcgctgttg gaagcggtgc tgttcgcctt cctgttcgcc ctggggcggg
     2941 acttggggct tttggcggcg ggggaggacc tggcccaggc caagggggtg ccgccgaaac
     3001 gcaccatcct ggcctccgtg atggcggcct cgctcctcac cagcgcggtg gtggccgtga
     3061 ccgggcccat cgccttcgtg gggctcatcg tgccgcacct ctaccggctc ttcacgggca
     3121 acgaccaccg gttgctgctg gcgtccagcg ccctgggcgg ggccgctttc ctgatggtct
     3181 gcgacagcgc ggcccggtcc tttttcggcc ccaccgagct gcccgtgggg gtgctgacgg
     3241 cggtc
//
LOCUS       DASTJJ010000584         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_81276, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000584 DASTJJ010000000
VERSION     DASTJJ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81276"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1135
                     /gene="pstC"
                     /locus_tag="VFR02_07185"
     CDS             146..1135
                     /gene="pstC"
                     /locus_tag="VFR02_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9870260.1"
                     /translation="MSGNELTKDQEAGKPAFWFFNFSRSQAIGRGDRIFKASTAFFAF
                     LVLALAMLTVGALFYNSIPCFKAMGWRFLVTSDWDPIQNVFGAVPFVYGTLVSSAIAL
                     LIAAPLGLGISLFLTELAPMKLREPVAFLVEILAAIPSVVYGLWGMFVLAPIMRTWVD
                     PVLHRVIGGPFFGEGATELSLFTAGSILSIMILPTITAITREVFEAVPNVYRESARAL
                     GATSWETIQLSVLKSSKPGIVGALMLGLGRALGETMAVTMVIGNSPQVTANLLAPGHS
                     MASVIANEFTEATGDVYLAALSEIGLLLMGITLVLNIAAHLLVWLTTRNYARN"
     gene            1137..>1945
                     /gene="pstA"
                     /locus_tag="VFR02_07190"
     CDS             1137..>1945
                     /gene="pstA"
                     /locus_tag="VFR02_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9870261.1"
                     /translation="MRFDFQTRRALLNRLMVGLCGMAAFVVFIPLGDLLWYVLAQGVQ
                     GMGPLGIVVSLVVAAILVAIPVVVFRYGKRYHWAGYLATALAAWWSLDHLHQLWVIAS
                     KIDWKDISFFIHRPTPVGVPGGGMANAIVGTITLILIASLVGLPVGIFAGVYLAEFGK
                     KGRFAWLVRFTSDVLSGVPSIVTGIVAYTVIVRTMGGFSALAGGVALGFMMIPIVTRT
                     TEELVKLVPHSLREASWALGIPQWRTILQIIIRTAKNGILTGVLLSIARVSG"
BASE COUNT          281 a          642 c          613 g          409 t
ORIGIN      
        1 tggtggacct gctgaactgg atgatggaca agggcaacag cgaggtctcc tcgttgtact
       61 acgccccctt cccggacagc ctggtggcca aggtgaaggc cgccgtcgcg accatcaagt
      121 actgatccga ggtcttgtgg aaactttgag tggaaatgag ctgacgaagg accaggaggc
      181 cgggaaaccg gccttctggt ttttcaattt cagccggtcc caggccatcg gccggggtga
      241 ccggatcttc aaggcttcca ccgccttctt cgcctttttg gtgctggccc tggcgatgtt
      301 gacggtgggc gctttgttct ataactccat cccctgtttc aaggccatgg gatggaggtt
      361 cctagtgacg tccgattggg acccgatcca gaacgtcttc ggggcggtcc ccttcgtgta
      421 cgggaccctg gtctcttccg cgatcgcctt gctcatcgcc gcccccctgg ggttggggat
      481 ctctctgttc ctcactgaat tggcccccat gaagctgcgg gagccggtgg ccttcctggt
      541 ggagatcctg gcggccatcc cctcggtggt ctacggcctt tggggcatgt tcgtgctggc
      601 ccccatcatg cgcacttggg tggaccccgt cctccaccgg gtcatcggcg ggcccttctt
      661 cggggagggc gccaccgagc tctcgctctt cacggcgggc tccatcctat ccatcatgat
      721 cctgcccacc atcacggcca tcacccggga ggtcttcgag gcggtcccga acgtctaccg
      781 ggaatcggcc cgcgccttgg gcgccaccag ttgggagacc atccagcttt ccgtgctcaa
      841 atcctccaag cccggcatcg tgggggccct catgctgggg ctgggccgcg ccctggggga
      901 gaccatggcg gtgaccatgg tcatcggcaa cagcccccag gtgaccgcca acctgttggc
      961 gcccggccac agcatggcca gcgtcatcgc caacgagttc accgaagcca cgggcgacgt
     1021 gtacctggcg gccctttccg agatcggcct gctcctgatg ggcatcacct tggtcctcaa
     1081 catcgccgcc cacctgttgg tctggctcac cacccgaaat tacgcgagga actgacatgc
     1141 ggttcgactt ccagacccga cgggccctct tgaaccgcct catggtgggg ctttgcggca
     1201 tggcggcctt cgtcgtcttc atcccgctgg gcgacctgct ttggtacgtc ctggcgcagg
     1261 gggtccaggg gatggggccc ctgggcatcg tggtctccct ggtcgtggcc gccatcctgg
     1321 tggcgatccc cgtggtggtg ttccgttacg gcaagcgtta ccactgggcg gggtacctgg
     1381 ccacggcact cgcggcctgg tggtccctgg accatctcca ccagttgtgg gtcatcgcct
     1441 cgaagatcga ttggaaggac atcagcttct tcatccaccg gcccaccccc gtgggggtgc
     1501 cgggaggcgg catggccaac gccatcgtgg ggaccatcac cctcatcctc atcgcctccc
     1561 tggtggggct gcccgtgggc atcttcgcgg gcgtctacct ggccgaattc ggcaagaagg
     1621 ggcgtttcgc ctggctggtg cgtttcacct cggacgtgct gtcgggggtc ccttccatcg
     1681 tgaccggcat cgtggcctat acggtcatcg tgcggaccat gggggggttt tccgccttgg
     1741 cgggcggcgt ggccctggga ttcatgatga tccccatcgt gacccgcacc acggaggaac
     1801 tggtgaaatt ggtgccccac tccctccggg aggcctcctg ggccctgggg atcccccaat
     1861 ggaggaccat cctgcagatc atcatccgca cggccaagaa cggcatcctg accggggtgc
     1921 ttttgtccat cgcccgggtg tcggg
//
LOCUS       DASTJJ010000585         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_81314, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000585 DASTJJ010000000
VERSION     DASTJJ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81314"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            887..1264
                     /locus_tag="VFR02_07195"
     CDS             887..1264
                     /locus_tag="VFR02_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870262.1"
                     /translation="MRSARATTLYVIWNRGLPLEDIGVLGDYSAFKRILFYWMNDWQR
                     DPDSLEMLRRHGMTDPLRDMVGRQDVYLILPDNYHSLLGYYLREKLRLDPTLVEVHQG
                     TLFNIYRVEGKPLFGTKKTTTGA"
     gene            complement(1272..1619)
                     /locus_tag="VFR02_07200"
     CDS             complement(1272..1619)
                     /locus_tag="VFR02_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870263.1"
                     /translation="MKLISPKFHGYLDYLVVAVFAAAPSFFHFSMLPMAVSYALAVIH
                     LALTLLTDFPLGAFKVVPLPWHGIIEMVVGPCLAALPFAFGMTAEPDAEYFYVAAGAV
                     IFLAWSLTDYGRK"
BASE COUNT          312 a          549 c          524 g          328 t
ORIGIN      
        1 tttttccttt cggcggcggg gtggatcctc ctcttccggg ggcccctcac cccgcgcggc
       61 gcggccgccg gggcgggtct tgtcttcgcc tcctggtgcg tccggccgga agcgacgttc
      121 ctctccggcg tcgtcctgat cccctgggcc ttcgcgcaat ggcggctgga agcccccgcc
      181 tccaggcggc gcaaggccct tcttttggcc ggcctgggcg ccctcctcct ggccgccacg
      241 gccctggacc gcagccattg ggcccaggac ccggcgggac gggacgtggc cccctatatc
      301 aacaccatgc acggcatcgt ggaatatcgg gaactggaac ccgggccggg aacggatcgg
      361 cggttggccg gcgtaggatg ggattctctc gacctgacca tgtacggcaa ccgcgactgg
      421 gacaaccccg cctatagctt ggagagggcg cgccgcctcc tggaacaatt gggcccggct
      481 tggtcgggga aaggcctggg ttggagatgg attttccaca gcgacttcat gcgcttccaa
      541 ctttcctgcc tcttggccct ggccttgttc ttaggggcgc gggaccgtcc ctggcttctt
      601 tacaacggcc tctggatatt ctcgatcttc ctggggatct attacgaaat gaagatcacc
      661 gaatgggtgg tttggcccct tttctccctt tggggcatcc tgggcttttt gggcgcgccc
      721 gcctccgggc ccgaaccccg tttttccggg ctcgccgcgc gccttcccgt cgttcgaccc
      781 gtcgtccttc ttttggccgc gctgtgcgcc gccaccctcc ttcattcgga cttcgtggag
      841 aatcaaaggg ctcaaacgcg ggaagcggcc ctacaacaag acctccgtgc gctccgcccg
      901 ggcgacgact ctttatgtga tctggaaccg ggggctaccc ctggaagaca tcggcgtcct
      961 gggcgactat tcggccttca agcgcatcct tttttactgg atgaacgact ggcaaaggga
     1021 cccggactcg ctggaaatgc tccggcggca cgggatgacg gaccccttgc gggacatggt
     1081 cggccgtcag gacgtttatt tgatcctgcc ggacaattac cattcgcttt tgggctatta
     1141 cctacgggag aaactgcgcc tggaccccac cctggtcgag gtccatcagg ggaccctctt
     1201 caacatctac cgggtggagg gaaaaccact tttcgggaca aagaagacca ccaccggggc
     1261 ctgaacgccg ctcacttgcg gccgtaatcc gtgagcgacc aggccaggaa gatcaccgcg
     1321 ccggcggcga cgtaaaaata ctcggcgtcg ggctccgcgg tcatcccgaa agcgaaaggc
     1381 agggccgcca ggcaaggtcc aacgaccatc tcgatgatcc catgccacgg caaggggacc
     1441 accttgaagg cgcccagggg aaaatcggtc aggagcgtca acgccaagtg gatgacggcc
     1501 aacgcgtagg aaacggccat cggcaacatc gagaaatgga aaaaggaagg cgccgcggcg
     1561 aagaccgcca cgaccagata atccagataa ccatggaact tcgggctgat gagcttcatg
     1621 cgtcctcccc ccaaaaggtt cactgacacc ttcatttagc ctttggcggg gcctgaagtt
     1681 accggaaaag ggcggcttct aaagaagggg gag
//
LOCUS       DASTJJ010000586         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_813746, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000586 DASTJJ010000000
VERSION     DASTJJ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813746"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1708
                     /locus_tag="VFR02_07205"
     CDS             18..>1708
                     /locus_tag="VFR02_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870264.1"
                     /translation="MTSGAPNQGSGITGATASGPGGLQVYGSQLFLADTGNNRVLIFN
                     TLPTASGAPADLALGQPNLNSGTSGSANLYNSPNDVFFDGVNFFVSDTGNNRVLVYAG
                     VPTGYNPTPSAILGQQSLTANSPNQGGSVANNTLYFPRGLWSNGATLVVADSANNRVL
                     FFTSPFSTNEQASLVLGQTGFSAAVSNQGNASPSSSTLNSPYGVWFDGTRLYVGDTFN
                     NRVLAYNGMPVTNGATAALVLGQTGFSAQSPNQGGSTPNAGTFYYPNRIFSNGSSLFV
                     ADGGNDRVLVFNSAPVTSGTPAGNVLGQPNFTSNSSNEGGCTGSSTLDVPAGVYYDGS
                     RLFVSDNYNNRTLVFVNATATPTPTPTLTLTPLPSCPGGNGSVGVGTSGFVTTGGTQS
                     VSGQTAVLAVTFPGPTYVTDIRVPGLNTGQVFQVGIYADNGSGTAPTSLLAAASYTDT
                     GSSSITVPFGSAVPVAGIKWLAFAPSVFVNVPSCSNLGAVFNGAMPAVASAPTTYLQG
                     PNLSADYVCLATPTPTLTATPTLTSSVTVSPTVTLSETPTPTVTLTYTQTPTITLT"
BASE COUNT          282 a          623 c          520 g          283 t
ORIGIN      
        1 agatcggcca ggcggggttg acgagcgggg cccccaacca ggggtcgggc atcacggggg
       61 ccaccgcctc gggcccgggc ggcctgcagg tctatgggag ccaattgttc ctggccgata
      121 cgggcaacaa ccgcgtgctg atcttcaaca cgctgccgac cgccagcggc gcgcccgccg
      181 acctggcgtt gggacagccg aacctgaatt caggcacgtc gggttcggcc aacctgtaca
      241 actcgcccaa cgacgtcttt ttcgacgggg tgaacttctt cgtttccgac acgggcaaca
      301 accgggtcct ggtctacgcg ggggtgccca cgggatacaa ccccaccccc tcggcgatcc
      361 tgggccagca aagcctcacc gccaattcac ccaaccaggg ggggtcggtg gccaacaaca
      421 ccctttactt tccccggggc ctctggagca acggcgccac cttggtggtg gcggattcgg
      481 ccaacaaccg ggtgcttttc ttcaccagcc ccttttccac caatgaacag gccagcctgg
      541 tcctgggcca gaccggcttt tccgcggccg tctccaacca ggggaacgcc agcccctcgt
      601 cctccacctt gaactcgccc tacggggtct ggttcgacgg cacccggctc tacgtggggg
      661 ataccttcaa caaccgggtg ctggcctaca acgggatgcc ggtcacgaac ggggccaccg
      721 ccgccctggt cttgggccaa acgggttttt cggcgcagag ccccaaccaa gggggatcca
      781 cccccaacgc cgggaccttt tactacccga accggatctt cagcaacggg tcctcgctct
      841 tcgtggcgga cggggggaac gaccgggtgt tggtcttcaa cagcgcgccg gtcaccagcg
      901 gcacgccggc gggcaatgtc ctgggccagc ccaatttcac ctccaatagc tccaatgagg
      961 ggggctgtac ggggtcgtcc accctcgacg tgccggcggg ggtctattat gacgggtccc
     1021 ggctcttcgt cagcgacaac tacaacaacc ggaccctggt gttcgtcaac gccacggcca
     1081 ccccgacccc cacgccgacc ttgacgctca cgcccctgcc gtcctgccct ggaggcaatg
     1141 ggagcgtggg ggtcgggacg tccgggttcg tgaccaccgg cggcacccag agcgtctcgg
     1201 gtcagacggc cgtcctggcg gtgaccttcc cgggccccac ctacgtgacg gacatccggg
     1261 tgccgggcct gaacacgggc caggtcttcc aagtggggat ctacgcggac aacgggtcgg
     1321 ggaccgcccc gacgagcctc ctggccgcgg cgagctacac ggatacgggg tcctccagca
     1381 tcaccgtccc cttcgggagc gcggtgccgg tcgccgggat caagtggttg gccttcgccc
     1441 cctcggtctt cgtcaacgtg ccttcctgtt ccaacctggg cgcggtcttc aacggagcca
     1501 tgccggcggt ggcttcggcg ccgaccacct acctgcaggg gcccaacctg tccgcggatt
     1561 atgtgtgcct ggccaccccg accccgacct tgaccgccac gccgaccttg acctcgtcgg
     1621 tgacggtgtc gcccaccgtc actttatcgg agacaccgac ccccacggtg accttgacct
     1681 acacccaaac gccgaccatc accctgac
//
LOCUS       DASTJJ010000587         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_813755, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000587 DASTJJ010000000
VERSION     DASTJJ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813755"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFR02_07210"
     CDS             complement(<1..231)
                     /locus_tag="VFR02_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870265.1"
                     /translation="MKTNPKRSWLGKLWRHPMAGLAVVLLLGGLFRFYNPDWDLGHSF
                     HPDERNILGQTAGLQASNGYRVQFFAYGQLPVF"
     gene            complement(308..1090)
                     /locus_tag="VFR02_07215"
     CDS             complement(308..1090)
                     /locus_tag="VFR02_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9870266.1"
                     /translation="MEKIIAVVPVFNEEKTLRDILGRIRRQTDLLVCVNDGSTDASMR
                     ILREEMGKSGKGYVVDLPRNLGMAGALRRGFEFVLYLRDKKVVRDGDWVVTIDADGQH
                     EPEYIRPLVRYAGRRRLEVLLTRRDFSVYPIYKRLGNRFLTWTNSRLAGFPYRDVESG
                     LRLLRVGVLERILAYYTGVQYSCAQEIALISAREGCRIDNDYLVRIAYYRPGTTVWDG
                     FIVLALSLYAYGRWKLKLRNRPQADRALMAKAFRAFRRKPKD"
BASE COUNT          269 a          402 c          356 g          228 t
ORIGIN      
        1 aaagaccgga agctggccat aggcgaaaaa ttggacccgg tacccgttgg aggcctgcag
       61 accggccgtc tgccccagga tattgcgctc gtcggggtgg aaagaatggc ccaggtccca
      121 atccgggttg tagaaccgga aaagcccgcc cagcaggagc accaccgcca accccgccat
      181 cggatgccgc cagagtttgc ccagccacga acgcttcggg ttggttttca cgaaagggtc
      241 ctcggaaagg ggatgaaaat catcaagaca aaagcggtta atagtataac cggtttccgg
      301 cttcttttca gtctttcggc ttgcgccgga aggcccggaa agccttcgcc atcagggccc
      361 gatccgcctg cggacggttg cggagcttga gcttccagcg cccataagcg taaaggctca
      421 gggccagcac gatgaatccg tcccaaaccg tcgtgcccgg ccggtaatag gcgatcctca
      481 ccagatagtc attgtcgatc cggcatcctt cgcgggccga gatcaaggcg atctcctggg
      541 cgcaggaata ttgaacgccc gtgtaatagg ccaggatccg ctccagcacc ccgacccgca
      601 aaaggcgcag ccccgactcc acgtcccggt aagggaaccc ggccaaccgg gaattggtcc
      661 aggtcaagaa ccggttcccc agccgcttat agatcggata aacggaaaaa tcccgccggg
      721 tcagcaggac ctccaaccgc cgccggcccg cgtaccggac cagcgggcgg atgtactcgg
      781 gctcgtgctg cccgtccgcg tcgatggtga ccacccaatc gccgtcgcgg acgacctttt
      841 tatcccgcag atacagcacg aactcaaaac cccgccgcaa ggcccccgcc atcccgaggt
      901 tccggggcag gtccaccacg tagccctttc cggacttccc catttcctcc cgcaggatcc
      961 gcattgaggc atcggtggat ccgtcattga cgcagaccag caggtccgtt tggcggcgga
     1021 tgcggcccag gatgtccctc aaggtctttt cctcgttgaa aacagggacc accgcgatga
     1081 tcttttccaa gctattcccc ggtcaaaaaa aaagcccgaa tccccggaag gattcgggct
     1141 tttttgattt ctctagaaca aaaattcctc ataggccttg ctcaggtcgg tcgtcggcag
     1201 gtggctggcg tcctgatagg gggcctgcgc cggggcgtac tttttcaaca tgggc
//
LOCUS       DASTJJ010000588         1176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1672014, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000588 DASTJJ010000000
VERSION     DASTJJ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672014"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFR02_07220"
     CDS             complement(<1..364)
                     /locus_tag="VFR02_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9870267.1"
                     /translation="MRPAQKIEAPLPPNLVLIGMFGSGKSTVGRLLAEALRYHFVDVD
                     HLIEARYRMPLQKVLDKLGMKGFMRMEEATLLALRSRRCVVSPGGSAVYYAKAMRHLG
                     TLGPRIYLRVPLPELKRRL"
     gene            complement(428..>1176)
                     /locus_tag="VFR02_07225"
     CDS             complement(428..>1176)
                     /locus_tag="VFR02_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870268.1"
                     /translation="RVTRLCLKGRFDRLQAGVGLQDMGWGQGWFYRPTDYFEPLGPLA
                     FWREEPLGSESLDLSQPLFDELVLEAAARATAGGGDWVVRLPNETLAYALTPSLARLG
                     GRMVLGLEARLTLPVCQLRLEGARWDGGDSPLEMEAGLSTRWDGSDLCLEWLRDATGE
                     ALGEFSGAAPGADYLYFSWGRALGPAWRLETRLVKSPDGGPFLLWPKAVMDFGTGWQF
                     SLEGRTLVAWSAGPLARVGARLFTALAWEF"
BASE COUNT          199 a          415 c          411 g          151 t
ORIGIN      
        1 gcagtcgccg cttgagctcg ggcaggggga ccctcaggta gatccggggc cccagcgtgc
       61 ccaggtggcg catggccttg gcgtaataga ccgcgctgcc gccgggcgag accacgcaac
      121 gccgggaccg cagggccagc agcgtggctt cctccatgcg catgaagccc ttcatgccca
      181 gcttgtccaa gaccttctgc aggggcatgc ggtagcgggc ctcgatcagg tggtccacgt
      241 ccacgaaatg gtagcgcagg gcctcggcca gcaggcggcc caccgtgctc ttgccgctgc
      301 cgaacatgcc gatgagcacc aggttcgggg ggagcggggc ttcgatcttt tgggcgggtc
      361 gcatggcctt ccttgaaggg ggatgggggt caggcgcccc aaggataaca aaaatccgcc
      421 ggagggccta gaactcccag gccagggcgg tgaagagccg ggcccccacg cgggccaggg
      481 ggccggccga ccaggcgacc agggtccttc cttccaggct gaactgccaa cccgtgccga
      541 agtccatcac ggccttgggc cagagcagga agggaccgcc gtcgggcgac ttcaccaggc
      601 gggtctccag gcgccaggcc gggcccaggg cccggcccca gctgaaataa aggtagtccg
      661 ccccgggggc ggcgccggaa aattccccca gggcttcccc cgtggcgtcg cgcagccatt
      721 ccaggcaaag gtcgctgccg tcccaacggg tggacaagcc ggcctccatt tccagcggcg
      781 aatccccccc gtcccaccgc gcgccttcca ggcgcagttg gcagaccgga agcgtcaagc
      841 gggcctccag ccccaagacc atgcgccccc ccagccgcgc caggctgggc gtgagggcgt
      901 aggcgagggt ctcgttgggg agccggacca cccagtcgcc gccgcccgcg gtggcccgcg
      961 ccgccgcctc caggaccagt tcgtcgaaaa ggggttggga aaggtcgagg gactcgctgc
     1021 ccaggggttc ctcgcgccag aaggccagcg gccccagggg ctcgaaataa tccgtgggcc
     1081 ggtaaaacca gccctgtccc cagcccatgt cctgcaggcc gaccccggcc tgcaggcggt
     1141 cgaagcggcc cttcaggcaa agccgggtga cgcgga
//
LOCUS       DASTJJ010000589         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_814246, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000589 DASTJJ010000000
VERSION     DASTJJ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814246"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1080
                     /gene="hisG"
                     /locus_tag="VFR02_07230"
     CDS             205..1080
                     /gene="hisG"
                     /locus_tag="VFR02_07230"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET9870269.1"
                     /translation="MPNQLKLGLPKGSLQESTFHLFAKAGYRISSTSRSYFPNIDDAE
                     IKPMLIRAQEMSRYVEDGTLDVGLTGRDWVQENGSDVVEIAELIYAKQGLRPVRWVLA
                     APAGSPIKSVKDLQGKKIATELVNVSKQYLKKNGVTASVEFSWGATEAKPPELADAII
                     EVTETGSSLRANNLVILETILESNTVVVVNKAAWDDPWKKKKVQDMVLMLKGALAAEE
                     KVGLKMNVPRKSLEAVLKVLPALNSPTVMELHDKDWVDVDTVVDEKTVRQIAPKLKDA
                     GAEGIVEYPLNKVIP"
     gene            1098..1598
                     /locus_tag="VFR02_07235"
     CDS             1098..1598
                     /locus_tag="VFR02_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870270.1"
                     /translation="MPSPAELNKEAVELYRAQDLQGAEELYREALRQDPSYFPSQLNL
                     GILLFKAGRYDEAIKECTKAVSLKPDHPSAHFHLGNAYYAKMWWEEALVEYERVFALD
                     PSHVDVHFALGGIYLNRGHKEKAVECWQKYLGLAPADSPKAKLAEEYVKGAIANQVHI
                     GKYIAE"
BASE COUNT          356 a          522 c          510 g          268 t
ORIGIN      
        1 tgcaggtgga tggtttccgg caaggcgggc tcgagtctcc ttcgtcaagt ggcgtcattc
       61 tagggcgggc cgcgccaaat ggaaagcgcc gcccggccct tttcgggccg tccaaacaaa
      121 tcttgccaaa cccggaggca ggctgttaaa taccagggat ttcgggggcc ccggtcccgc
      181 gcgccccatt caaaaggaaa aaccatgccc aatcagttga agctcggatt gcccaaggga
      241 agcctgcagg agtccacctt ccaccttttc gcgaaggccg gctaccgcat ctcctccacc
      301 tcccggtcct atttccccaa catcgacgac gccgagatca agcccatgct gatccgcgcc
      361 caggaaatga gccgctacgt cgaggacggc accctggacg tggggctcac cggccgggac
      421 tgggtgcagg agaacggctc cgacgtggtg gagatcgccg agctcatcta cgccaagcag
      481 ggcctgcgcc ccgtgcgctg ggtcctggcc gcgccggcgg gttcgcccat caagtcggtc
      541 aaggacctgc agggcaagaa gatcgccacc gagctggtga acgtgtccaa gcagtattta
      601 aagaagaacg gcgtcaccgc cagcgtggaa ttctcctggg gggccaccga ggccaagccg
      661 cccgagttgg ccgacgccat catcgaggtc accgagaccg gctcgtccct gcgcgccaac
      721 aacctggtca tcttggagac catcctggaa tccaacaccg tggtggtggt caacaaggcg
      781 gcctgggacg acccctggaa aaagaagaag gtccaggaca tggtgctcat gctcaagggc
      841 gccctggcgg ccgaggagaa ggtgggcctg aagatgaacg tgccccgcaa gagcctggag
      901 gccgtgctga aggtgctgcc ggccctgaac tcgcccaccg tcatggagtt gcacgataaa
      961 gactgggtgg acgtggacac cgtggtggac gagaagaccg tgcggcagat cgcgcccaag
     1021 ctaaaagatg ctggcgccga ggggattgtg gagtatcctt tgaacaaagt gatcccctga
     1081 cggtctgacg agggtttatg ccgagtcccg ccgaactgaa caaggaagcc gtggagcttt
     1141 accgggccca ggatctccag ggcgccgagg aactgtaccg ggaagcgctc cggcaggacc
     1201 cttcttattt cccctcccaa ttgaacctcg ggatattgct cttcaaggcc ggccgttacg
     1261 acgaggccat caaggaatgc accaaggccg tgtcgctcaa gcccgaccat ccctcggcgc
     1321 acttccacct gggcaacgcc tattacgcca agatgtggtg ggaagaggcc ctggtcgagt
     1381 atgagcgggt cttcgcgctg gacccttccc acgtggacgt gcatttcgcc ctgggcggca
     1441 tctacctcaa ccgcggccac aaggaaaagg ccgtggaatg ctggcagaag tacctggggt
     1501 tggccccggc ggattcgccc aaggccaagc tggccgagga atacgtgaag ggcgccatcg
     1561 ccaaccaggt ccacatcggc aagtacatcg ccgagtgacc aagtctcaac caacaaaaaa
     1621 atccaacgcc acgcccaagg gcggcccctt tccccc
//
LOCUS       DASTJJ010000590         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_814270, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000590 DASTJJ010000000
VERSION     DASTJJ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814270"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFR02_07240"
     CDS             complement(<1..510)
                     /locus_tag="VFR02_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9870271.1"
                     /translation="MAKIALVTGANRGIGFEVARQLGKAGMTVILGSRDREKGAEAHS
                     VLDKEGLRVRSQLLDLLNPGTARMAADFIGVKYGRLDVLVNNAGGFYDQEATPSKTSL
                     KYLREALENNLIGTWSVIQAMLPLLLKSSSARIVNVSSGMGSYGDPQTGLGTLGGAAP
                     AYALSKLALN"
     gene            616..>1127
                     /locus_tag="VFR02_07245"
     CDS             616..>1127
                     /locus_tag="VFR02_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9870272.1"
                     /translation="MPKLSMFNSISLDGYFTDAKNDMSWAHQRAQDPEWDDFVKGNAS
                     GKGRLLFGRVTYQMMEAFWPNQGIQMMPEVAKGMTASPKVVFSRTLDKVTWENTVLLK
                     GDLVEEVKKLKSAPGPDMVILGSGSIVAQLAKENLIDQYQIVISPIALGAGRTLFEGL
                     KKNLGLKLVK"
BASE COUNT          229 a          353 c          334 g          211 t
ORIGIN      
        1 gttcaaagcc agcttggaaa gcgcgtaagc gggcgccgcg ccgcccaggg tccccaaccc
       61 ggtctggggg tcgccatagg accccatgcc gctggaaacg ttcacgatgc gcgccgagga
      121 actttttagg agcaggggca gcatggcctg gatcacgctc caagtgccga tgaggttgtt
      181 ctcgagcgct tcccgcaggt atttcaggct ggtcttggaa ggcgtggcct cttggtcgta
      241 aaagccgccc gcgttgttga ccaaaacgtc cagccgcccg tatttgaccc cgatgaagtc
      301 ggcggccatg cgggccgtgc ccgggttcaa gaggtccaac agttgggacc tcacccgcag
      361 gccttcttta tccaacaccg aatgggcctc cgcgcccttt tcccggtccc gcgaccccag
      421 gatgaccgtc atccccgcct tgcccaactg ccgtgccacc tcgaacccga tgccccggtt
      481 cgcccccgtc accaacgcga tcttcgccat ggttccgcct ccctgatgcc cccaaaagca
      541 taggcccaag ggactggttt ttaccggcgc ccaaggttac ctttgggtta aatcgcaact
      601 tttccggagg ggttcatgcc gaaactcagc atgttcaatt cgatcagcct ggacggctat
      661 ttcaccgacg ccaagaacga catgagctgg gcccaccagc gggcgcagga ccccgaatgg
      721 gacgatttcg tgaagggcaa cgcctccggc aagggccgcc tgctcttcgg ccgggtgacc
      781 taccagatga tggaggcctt ttggcccaac cagggcatcc agatgatgcc ggaggtggcg
      841 aagggcatga cggcgagccc caaggtcgtc ttttccagga ccctcgataa agtgacctgg
      901 gagaacaccg tcttattgaa gggcgacttg gtggaagaag tgaaaaagct gaagtccgcc
      961 cccggccccg acatggtcat cttgggaagc ggaagcatcg tggcccaatt ggcgaaggag
     1021 aacctgatcg accaatatca gatcgtcatt tcccccatcg ccctcggcgc cggccgaacg
     1081 ctatttgagg ggctgaagaa gaacctgggc ttgaagttgg tgaagga
//
LOCUS       DASTJJ010000591         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_814497, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000591 DASTJJ010000000
VERSION     DASTJJ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814497"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1148
                     /locus_tag="VFR02_07250"
     CDS             <1..>1148
                     /locus_tag="VFR02_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870273.1"
                     /translation="PSFTFEAVVVSTEGSCGISLNPMMMALGPEGNVYVTDQGNRYVV
                     FDPTSTPWAFIHECGSFGSASGQFSLPIGIGVDAARRVYVSDDLNNRVERFLPCLTQT
                     PQPTCVPTDTPTVTFTPSPASPSPTPSPSLTPTVTITPTPSPAPACGYQGSLVLGSQG
                     TGFGQLESPFDEATDPSGRIYVVDGQNRVTEWSPLPAPVAVATWTLPAPTGGVCHLAA
                     DANHLYVSSQGSDTIQILNLPSGTPVGPPLGGPGTGAGQFDSLWGIAVDAAGDIYAAD
                     NGNGRVEKFTAPGYAPSVFVDNLGAAPTGVGVDSQGNVFVSDGTGRVRKYDPSGTLQG
                     TIAGPGIGTDPMGNFSFDLAVDGCDRVDVLDTNNNRVLVYGPGGAPLLA"
BASE COUNT          183 a          400 c          362 g          203 t
ORIGIN      
        1 ccttccttca cgttcgaggc cgtcgtggtc tcgaccgagg gctcctgcgg catcagcctc
       61 aatcccatga tgatggcgct gggtcccgaa gggaacgtgt acgtgaccga tcaggggaac
      121 cgctatgtgg tgttcgatcc cacatccacc ccttgggcct tcatccacga atgcggatcc
      181 ttcggttcgg cctcgggaca gttcagcctc ccgatcggga tcggggtgga cgccgcccgc
      241 cgggtctatg tgtccgacga tctcaacaac cgggtggaac gtttcctgcc ttgcctcacc
      301 cagacccccc aacccacttg cgtgccgacg gataccccga ccgttacgtt caccccgagc
      361 cccgccagcc cctcgcccac gccttcccct tccttgaccc cgaccgtcac gatcaccccg
      421 accccctctc cagccccggc ttgcgggtat cagggatccc tcgtgttggg ctcccaaggc
      481 acgggcttcg gtcaattgga atcgcccttt gacgaagcga ccgacccctc cggccggatc
      541 tacgtggtgg acggccagaa ccgggtgacc gaatggagcc ccttgcccgc ccccgtcgcg
      601 gtcgccactt ggaccctgcc ggcgccgacc gggggagtct gccatttggc cgcggacgcc
      661 aaccatctct acgtgtccag ccagggaagc gataccatcc agatcctcaa cctcccgtcg
      721 ggcacgccgg tcggtccgcc cctcgggggg ccgggaacgg gggcggggca gttcgattcc
      781 ctttggggga tcgcggtgga cgcggcgggg gatatctacg cggcggataa cggcaacggc
      841 cgcgtcgaga aattcacggc gccaggatac gcgccgagcg tttttgtcga caacctgggc
      901 gccgcgccca ccggggtggg ggtggacagt cagggcaacg tttttgtctc ggacgggacg
      961 ggtcgtgtcc gaaaatacga ccccagcggg acgctccaag ggacgatcgc gggccccggc
     1021 atcgggaccg atccgatggg caacttcagc tttgacctgg cggtggacgg ttgcgaccgg
     1081 gtggacgtcc tggacaccaa taacaaccgg gtgttggtct atggaccggg cggtgcgcca
     1141 cttcttgc
//
LOCUS       DASTJJ010000592         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_814551, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000592 DASTJJ010000000
VERSION     DASTJJ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814551"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFR02_07255"
     CDS             complement(<1..1280)
                     /locus_tag="VFR02_07255"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HET9870274.1"
                     /translation="MMKRPKPYFSRLLLPFFLSLAGWTVQGAAPLRAQTFTQAAVNLT
                     NASPGQALTYVFNFSNASSLADCGDNFESYTVGGAPSASSWVSSGGTWTEVADNGPAS
                     TGTQAVSGVAATNSFPNLLNVCAGQVGDGSIQADVKIGAPVTQQATLLWRFTGSDTPS
                     FTNGSSYQVIVGIGFLVVGYYDVSANLFTSLGTVAEPSITVNTWHTVKLQATGSGVVT
                     LAVSLDGSPVTTVVSGSAPANTPTNPISSGFAGLQVNSGSAVAFDNVRIQRSAQIYGA
                     TITDTLPTGLAYVSSGQSAGVSAATVSGQRVVWSLGNPAASFSGSVTVTGTVNNCGIT
                     LSNTGEADTQTPKYTIVSNSVATTIIACTATPTKTPTATPTLTPTFTPTWTSTVTLTP
                     TVTLTPTVTRTFTWTATPTQTPTDTITLSPTVTLT"
     gene            complement(1461..>2018)
                     /locus_tag="VFR02_07260"
     CDS             complement(1461..>2018)
                     /locus_tag="VFR02_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET9870275.1"
                     /translation="GPYPPDELKAKGLLAPDTWVCPAGTQQVGQVSQNALLLPLLQAL
                     AASPGARDHCPRCRVSLTRADYEGAEIDRCPFCRGTLLRAGVLERLISRDSAAFSPDQ
                     IQQARVWRRTQKGAIAERDHFPAILCPLCATHMGKYVHSYLTQVIIDRCLNESCGAVW
                     CDGGELETIQMLVQDAKAGRDSEGP"
BASE COUNT          355 a          596 c          724 g          343 t
ORIGIN      
        1 gtcagggtca cggtcgggct caaggtgatc gtgtccgtcg gcgtttgggt cggggtcgcc
       61 gtccaggtaa aggtccgcgt caccgtgggt gtcagggtca ccgtcggggt caaggtcacc
      121 gtggacgtcc aggtcggcgt aaaagtcggc gtcagggtgg gcgtggccgt gggggtcttg
      181 gtgggggtgg ccgtgcaggc gatgatggtc gtggccaccg agttggagac gatggtgtat
      241 ttaggcgtct gggtgtccgc ttccccggtg ttcgacaagg tgatgccgca gttgttcacc
      301 gtgccggtga ccgtcaccga cccggagaac gaggccgccg gattccccag actccagacc
      361 acccgttggc cgctcaccgt ggccgccgag acccccgcgc tttgccccga ggagacatag
      421 gccaggcccg tgggcagcgt gtcggtgatg gtggccccgt agatctgggc cgaacgctgg
      481 atgcggacgt tgtcgaacgc caccgcactg cccgaattca cctgcaggcc ggcgaatccc
      541 gaggaaatgg ggttggtggg cgtattggcc ggggccgatc ccgacacgac cgtggtgacc
      601 gggctgccgt ccaacgagac cgccagggtg accacgcccg acccggtggc ctgcaacttg
      661 accgtatgcc aggtgttgac agtgatggaa ggttcggcga ccgtccccaa ggaagtgaag
      721 aggttggcgg aaacgtcgta atagcccacc accaggaaac cgatgcccac gatgacctgg
      781 taactggagc cgttggtgaa ggagggcgtg tcgctccccg tgaagcgcca caacagggtg
      841 gcttgttggg tgaccggggc gccgatcttg acgtcggctt ggatggaacc atcccccact
      901 tgacccgcgc agacgttcaa caggttgggg aagctgttcg tggccgccac gccgctgacc
      961 gcttgggttc ccgtggaggc ggggccgttg tccgccacct cggtccaggt gccccccgag
     1021 ctgacccagc tggaggccga gggggccccg ccgaccgtgt aggactcgaa attatccccg
     1081 cagtcggcca aggaagaggc gttcgaaaaa ttgaagacgt aggtcagggc ttggcccggc
     1141 gaggcgttgg tcaggttgac cgcggcctgg gtaaaggttt gcgcgcgtaa aggggccgcc
     1201 ccttggacgg tccaaccggc cagcgaaagg aaaaaaggca ggagcaacct gctgaaataa
     1261 ggttttgggc gcttcatcat gggatcggct tctttaaatt agaaaaagct tgcaggcctt
     1321 ttccctttcc aaggtcgttc ggctggagtg ctgaaaatta taccttttta tccgcgggcc
     1381 ggtagggggg gaccaggggg aaagggcccc gataccgccg aaaggaaccg gacccactac
     1441 atttggtagg gcgggtcggt tcagggcccc tcggagtcac ggccggcctt ggcgtcctgc
     1501 accaacatct ggatcgtctc cagttcaccc ccgtcgcacc agaccgcccc gcaggactcg
     1561 ttcaaacagc ggtcgatgat gacctgggtc aggtaggagt ggacgtactt gcccatgtgg
     1621 gtggcgcaaa gggggcaaag gatggcggga aaatggtccc gctcggcgat ggcgcccttt
     1681 tgggttcggc gccaaacccg ggcctgctgg atctggtccg gggaaaaagc ggccgagtcc
     1741 cggctgatga gccgctccag gacgcccgcc cgcaacaggg tgccccggca aaaaggacag
     1801 cggtcgatct ccgccccctc atagtcggcc cgggtcaggg agacccggca gcggggacag
     1861 tggtcccggg cccccgggga ggcggccagg gcctgcaaca gggggagcag cagggcgttc
     1921 tgggaaacct ggcccacctg ttgggtgccc gccgggcaga cccaggtgtc cggggccagc
     1981 aggcccttgg ccttcagctc gtcgggcggg tagggccc
//
LOCUS       DASTJJ010000593         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_82644, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000593 DASTJJ010000000
VERSION     DASTJJ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82644"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..608
                     /locus_tag="VFR02_07265"
     CDS             168..608
                     /locus_tag="VFR02_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9870276.1"
                     /translation="MRDTKILFILALAGSLVSMGCSGNKRIEGADAPLPPQTNEVAAA
                     PAPPPPAVEANPPSKYIVVKGDTLWGISGMSLAYHDNFQWPLIFKENRDQIKDPDLIY
                     PKQEFSINHDWSDADITKARNDASATPKYVPHTQPRETLPVDYF"
     gene            723..>1098
                     /locus_tag="VFR02_07270"
     CDS             723..>1098
                     /locus_tag="VFR02_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870277.1"
                     /translation="MTQAVSQRVTLEDHQECLRLFGEKDHTLKLLREAFALTLIARGN
                     ELLLSGGALEVAQASQTIERLRSHLRHHHEGYLSAAKVSQAIAEAKGEAAPEPEPTGR
                     ETILVSSRKKPISARTDNQQKYV"
BASE COUNT          232 a          375 c          323 g          168 t
ORIGIN      
        1 aaacgctcat cctcgtaaaa taaggctttt ttgggccggg gtgcttgacg gacccccccg
       61 gcctttcgat aatgaactta aggaaagcca aggtttcccg ggaccggaag gtcccggtgg
      121 gggcgtctga accgatagaa gcatcatctc atcggaggat acaaaaaatg cgtgacacca
      181 agatcctgtt catcctcgcc ctggcgggga gcctggtttc catgggctgc tcgggcaaca
      241 agcgcatcga gggcgccgac gcgccgctcc cgccccagac caatgaggtg gccgccgccc
      301 ccgccccccc gccgcccgcg gtggaggcca atccgcccag caagtacatc gtggtgaagg
      361 gcgacaccct gtggggcatc tcgggcatgt ccctggccta ccacgacaat ttccagtggc
      421 cgctgatctt caaggagaac cgggaccaaa tcaaggaccc ggacctgatc tatcccaagc
      481 aggagttctc gatcaaccat gactggtcgg acgcggacat caccaaggcc cgcaacgacg
      541 cctcggccac gcccaagtac gtgccccata cccagccgcg ggaaaccctg ccggtcgact
      601 acttttgacc ggacggtccg cgccgtcgaa cgagccgtgg gattaaaccg aaaactccct
      661 cttcccggcc ggggaaaggg agttttttat ttcgccgatg aggcgggaag gagcctttcg
      721 aattgaccca agccgtcagc cagcgagtca ccctggaaga ccaccaggaa tgcctgcgcc
      781 ttttcgggga gaaggaccac accctgaagc tgttgcggga agccttcgcc ctcaccctca
      841 tcgcccgggg caacgaactg ctgctctccg gcggggccct ggaagtggcc caggcctccc
      901 agaccatcga gcggctgcgg tcgcacctgc ggcaccacca cgagggctac ctgtcggcgg
      961 ccaaggtgtc ccaggccatc gccgaggcca agggggaagc ggccccggaa cccgaaccca
     1021 ccggccggga gaccatcctg gtctcctcgc gcaagaagcc catctcggcc cgcacggaca
     1081 accaacagaa atacgtgg
//
LOCUS       DASTJJ010000594         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_693425, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000594 DASTJJ010000000
VERSION     DASTJJ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693425"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="dxr"
                     /locus_tag="VFR02_07275"
     CDS             <1..786
                     /gene="dxr"
                     /locus_tag="VFR02_07275"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9870278.1"
                     /translation="VMAGEVVSRAAAKSGSRILPVDSEHCAVFQCLAGSKRGGEVHKI
                     ILTASGGPFRRLSARLFPKITLKRALRHPTWTMGPKITIDSATLMNKGLEVIEAHYLF
                     GTPYSKIDIVIHPESIIHSMVEYVDGSVLAQLGTTDMQIPIQYALTYPARAKAPVKRL
                     DLAGVGELHFEAPDRRKFPCVDLAYEAGRKGGTAPAVLNAANEVAVALFLKGAVSFVQ
                     IPRIIARVLARHRPLTNTTLDGILRADRWARDEALSAASPRIT"
     gene            802..>2091
                     /gene="rseP"
                     /locus_tag="VFR02_07280"
     CDS             802..>2091
                     /gene="rseP"
                     /locus_tag="VFR02_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET9870279.1"
                     /translation="MNPWFVVEVLLAFSFMIAIHEWGHFLAMRLSGVRVERFAIGFGP
                     TIFAKKWGDTEYALLAIPLGGYCKPMGGDLSDSSAGQMYQAPPKPGEFLYAPWWKRVF
                     IALAGPGMNYLSAFVIMFAILVAGEQVPIEKPLLGFVPPKSLAAQAGIRKNDLLVKID
                     GQDVTNLFTDEDKIYESLEKDPAQAVTLTLRRGAKTLQTTVQGDLKAKDADLGISSLT
                     EPVIGTVPLGTPARKAGLLPGDRVLSVDGKTVGDWNELAYRIRSAPSDAIALKVSRGG
                     KSYPVTLDRVYNGLYKAIGIAAPEPTAYEIKRLGPLQALGSSAERIQAMTVLYLQSLG
                     KLFTGKLSLRDNLSGPITIMRTMYQKAAQGWIEFASMVCFISLVLCLMNLLPIPVLDG
                     GQIVIFLVEGIKRGPVPVRWQALYQQVGFAFVILLMVL"
BASE COUNT          349 a          750 c          642 g          350 t
ORIGIN      
        1 gtcatggccg gggaagtggt ctcccgggcg gcggcgaagt cgggctccag gatcctgccg
       61 gtggacagcg agcattgcgc ggtcttccag tgcctggcgg gttccaagcg gggcggcgag
      121 gtgcacaaga tcatcctgac cgcctcggga ggccccttcc ggcgcctgag cgcccggctc
      181 ttccccaaga tcaccctcaa gcgcgcgctg cggcacccca cctggaccat gggccccaag
      241 atcaccatcg attccgccac cctgatgaac aagggcctgg aggtcatcga ggcccactac
      301 cttttcggca cgccctattc caagatcgac atcgtcatcc atcccgagtc catcatccat
      361 tccatggtcg agtacgtgga cggttccgtg ttggcccaac tgggcacgac cgacatgcag
      421 atccccatcc agtacgcgct cacctacccg gcccgcgcga aggccccggt gaagcgcctc
      481 gacctggcgg gcgtgggcga gctgcatttc gaggcccccg accgccgcaa gttcccctgc
      541 gtggacctgg cctacgaggc cgggcgcaag ggcggcacgg ccccggcggt cctgaacgcc
      601 gccaacgagg tggcggtggc ccttttcctg aagggggcgg tgtctttcgt gcagatcccc
      661 cggatcatcg ccagggtcct ggcccggcac cgccccctga caaacaccac cctggatggt
      721 atattgaggg ccgaccgctg ggcccgggac gaagcgcttt ccgcggcgtc cccccgcatc
      781 acctaatact caaggaaccc catgaacccc tggttcgtcg ttgaagtgct gctcgccttc
      841 agtttcatga tcgccatcca cgaatggggc catttcctgg ccatgcgcct gtcgggcgtc
      901 cgggtggaac gcttcgccat cggcttcggg cccacgatct tcgccaagaa atggggcgac
      961 accgaatacg ccctgttggc catccccttg ggcggctact gcaagcccat gggcggcgac
     1021 ctgagcgatt cctccgcggg ccagatgtac caggcgcccc ccaagccggg cgagttcctc
     1081 tacgccccct ggtggaagcg cgtcttcatc gccctggccg ggcccggcat gaactacctg
     1141 tcggccttcg tgatcatgtt cgccatcctg gtggccgggg aacaggtgcc catcgagaag
     1201 cccctgctgg ggttcgtccc gcccaagagc ctggccgccc aggcgggcat ccgcaagaac
     1261 gacctgttgg tcaagatcga cggccaggac gtgaccaacc tcttcaccga cgaggacaag
     1321 atctacgaaa gcctggaaaa ggaccccgcg caggccgtga ccctcaccct gcgtcgtggg
     1381 gccaagaccc tccaaacgac ggtgcagggc gacctgaagg ccaaggacgc ggacctgggc
     1441 atcagctccc tcaccgaacc cgtcatcggc accgtgccgc tgggcacgcc ggcccgcaag
     1501 gcggggctct tgcccggcga ccgggtgctg tcggtggacg gcaagaccgt cggcgattgg
     1561 aacgagctgg cctaccgcat ccgcagcgcc ccctcggacg ccatcgcctt gaaggtctcc
     1621 cgggggggca agagctaccc cgtcaccctg gaccgggtct acaacggcct ctacaaggcc
     1681 atcggcatcg ccgcccccga acccacggcc tatgagatca agcgcctggg gcccctgcag
     1741 gccctggggt cctccgcgga gcgcatccag gccatgacgg tcctctacct gcagtccctg
     1801 ggcaagcttt tcaccggcaa actttccctg cgggacaacc tgtcgggccc catcaccatc
     1861 atgcgcacca tgtaccagaa ggccgcgcag ggctggatcg agttcgccag catggtgtgc
     1921 ttcatctcgc tggtgctctg cctgatgaac ctgctgccca tccccgtgct ggacgggggc
     1981 cagatcgtga tcttcctggt ggagggcatc aaacgcggtc cggtgccggt gcgctggcag
     2041 gccctctacc aacaggtggg cttcgccttc gtcatcctcc tgatggtcct g
//
LOCUS       DASTJJ010000595         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_815124, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000595 DASTJJ010000000
VERSION     DASTJJ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815124"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..759
                     /locus_tag="VFR02_07285"
     CDS             46..759
                     /locus_tag="VFR02_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870280.1"
                     /translation="MTFRVKTFLAAAAIATLPFAPAMAGGSGTQYGSWGNGSGGAGQS
                     GSGSQIINGQINLNTNWSNLYGTVDTVAGDVVAQGAAAGNMIDVTTMNDTHVRNNQIV
                     GPQANIGSNISLDANNVWGGVGISNQVLCNGASVSTDPTLTQVSSHQSCRATDPYSSI
                     KTNITNIAGDAVVQGSSLGNSFEADSNAPNFPVVSHQFNNSGIVSNVQANTYNVGGSV
                     GLSSSAIGNTSQIIHYSTN"
     gene            1007..>1269
                     /locus_tag="VFR02_07290"
     CDS             1007..>1269
                     /locus_tag="VFR02_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET9870281.1"
                     /translation="MSALPIDIAAFDAATLAREPFAYAMVPYFVRPAAMAAINADYPL
                     VIHPGSFPLPTLKYGPAFRALIDAIEGPEFTAAVEKKLGVALKG"
BASE COUNT          239 a          434 c          352 g          244 t
ORIGIN      
        1 ccttcgttat gaacgcaacc caatcttctc tcaggagaca cttcgatgac cttccgcgtt
       61 aaaacctttc tggccgctgc cgccatcgcg acgctgccgt tcgcccccgc catggccggc
      121 ggttcgggta cccagtatgg cagttggggc aacggttcgg gcggcgccgg acaaagcggc
      181 tccggctccc agatcatcaa tggccagatc aacctgaaca ccaactggtc caacctctat
      241 ggtaccgtgg acacggtggc cggcgatgtg gtggcccagg gcgcggcggc gggcaacatg
      301 atcgacgtca cgaccatgaa cgacacccat gtgcggaaca accagatcgt gggaccccag
      361 gccaatatcg gctcgaacat cagcctggac gccaacaatg tgtggggcgg ggtgggcatc
      421 tccaaccagg tcctgtgcaa cggcgcttcc gtgtccaccg acccgaccct cacccaggtc
      481 agctcccatc agtcctgcag ggcgacagac ccctattcca gcatcaagac caacatcacc
      541 aatatcgccg gcgatgcggt ggtgcagggc tcgtctctgg gcaacagctt cgaagccgac
      601 tccaatgcgc ccaacttccc ggtcgtgtcc caccagttca acaattcggg catcgtctcc
      661 aacgtgcagg ccaataccta taatgtcggc ggcagcgtgg gcctgtcgtc cagcgcgatc
      721 ggcaacacct cgcagatcat ccattacagc accaactgag aaaaggtggc cgggttgcgc
      781 cggagcgcct aggggcaaat taggtgttcc ggacgggaga gagggccttt gggccctctc
      841 tcttttttta cccctccggc cttcgcaact ttgcgtgggc gatggctccc gctgtcgcgg
      901 gaacgccatg ccctcgcgtt gctacggcca cctccccttc ctgtgtgctg cgcacacgaa
      961 ggggaggctt ctcttttccc accaggcgcg gtaatttttg acccctatga gcgccctgcc
     1021 catcgacatc gcggcctttg acgccgccac cttggcccgc gagcctttcg cctatgccat
     1081 ggtgccctat ttcgtcagac ccgcggcgat ggccgccatc aatgccgact atcccctggt
     1141 gatccatccg ggcagctttc ctttgcccac cctgaaatac ggtccggcct tccgcgccct
     1201 gatcgatgcc atcgaaggac ccgaattcac cgccgcggta gagaaaaaac tgggcgtggc
     1261 cttgaaggg
//
LOCUS       DASTJJ010000596         1418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1305416, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000596 DASTJJ010000000
VERSION     DASTJJ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305416"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFR02_07295"
     CDS             <1..795
                     /locus_tag="VFR02_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9870282.1"
                     /translation="RNTLEHGLESAAERKALAKPAEGAFRLKASHLGGAFVLEVEDDG
                     RGIALDRVRARAKELGWIKPGEDPTPAALIDFLFKPGFAPLHAQGRVAGLEDMEQRVQ
                     RLKGSIRVQSQEGEGCRLILRLPKTLALIEGVLVRVGRKRYLLPMTQVRKISLSGVQE
                     YEEGPNGARWLRTPDGDYPVVDLNQWFGEAESGRGRVSLQVEAGRTRMCLLADEVMGK
                     QQVMMKGLGDALQSMPGISGGAILGDGKVGLILDVNALAQPSWDIA"
     gene            1089..>1418
                     /locus_tag="VFR02_07300"
     CDS             1089..>1418
                     /locus_tag="VFR02_07300"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9870283.1"
                     /translation="MAFNPNLPSTSPYFSFSPELMASLDPEGRFLEANPAWEGLSGLA
                     PAELVGTPFLERLHPADRPLALREIERLSRGKQETVRFETRVPDREGRLRHLAWTWTA
                     DPRQDRWY"
BASE COUNT          236 a          478 c          481 g          223 t
ORIGIN      
        1 cgcaacaccc tggagcacgg cttggaatcc gccgccgaac gcaaggccct ggccaagccc
       61 gccgaggggg ccttccgcct gaaggccagc cacctgggcg gcgccttcgt gctggaggtg
      121 gaggacgacg gccggggcat cgcgctggac cgggtccggg cccgggccaa ggaactgggt
      181 tggatcaagc ccggcgagga ccccacgccc gcggccctga tcgatttcct cttcaagccc
      241 ggcttcgcgc cgttgcacgc ccagggccgg gtggccggcc tggaggacat ggaacaacgg
      301 gtccaacggc tgaagggttc catccgggtc caaagccagg agggggaggg ctgccgcctg
      361 atcctgcgcc tgcccaagac cctggcgctc atcgagggcg tgctggtgcg ggtgggccgc
      421 aagcgctacc tcctgcccat gacccaggtg cgcaagatct cgctctcggg cgtgcaggaa
      481 tacgaggaag gccccaacgg cgcccggtgg ctgcgcacgc ccgacgggga ttacccggtg
      541 gtggacctga accagtggtt cggcgaggcc gagagcggcc gggggcgggt ctcattgcag
      601 gtggaggcgg gccgcacccg catgtgcctg ctggccgacg aggtcatggg caagcagcag
      661 gtcatgatga agggcctggg cgacgccctg cagtccatgc cggggatcag cggcggggcc
      721 atcctgggcg acggtaaggt ggggctcatc ctggatgtga acgccctggc ccagccttcc
      781 tgggacatcg cctaagccgc cgcccccagg cgaggatctt tcaccgcaga gacgcagagg
      841 cgcggggatc agaaatcatt tcaagatctt tttgtcgccg gcgtccaggg gttgccccgt
      901 tcccaatgat ccaatgttgg ggtagacgtc aaaaaccagg ggaataaata tgtttttctc
      961 cgcgtctccg cgcctctgcg gtgaaagggg ttttgacata ggttctaagt tcaccggcgc
     1021 ataaaaaaac ggacccccgg ccccggccgg ggtaaaatgg cgcctacttc catcctggga
     1081 cctgtccgat ggccttcaac cccaatctcc cgtccaccag cccctacttt tccttctccc
     1141 ccgagctgat ggcctcgctg gacccggagg gccgtttcct ggaggccaat ccggcctggg
     1201 aaggcttgtc gggcctggcc ccggccgaac tggtgggcac gcccttcctg gaacgcctcc
     1261 accccgcgga ccgtcccctg gcccttcggg agatcgaacg cctttcccgg ggcaagcagg
     1321 aaaccgtgcg gttcgagacc cgcgtgccgg accgggaagg ccgcctgcgc cacctggcct
     1381 ggacctggac cgccgacccc cggcaggacc gctggtac
//
LOCUS       DASTJJ010000597         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1793470, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000597 DASTJJ010000000
VERSION     DASTJJ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793470"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFR02_07305"
     CDS             <1..358
                     /locus_tag="VFR02_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HET9870284.1"
                     /translation="VVTNRDHLNGLKFSHSLPYAFTEYGAIMAANVLNSLEAVEMSVF
                     VVRAFVRMREKLTANQILEKRLAEIERKLLLHDTSLRDIYDRIRPLLLSPPEKPKRRI
                     GFTAEENRARYGAGKG"
     gene            368..>1627
                     /gene="carB"
                     /locus_tag="VFR02_07310"
     CDS             368..>1627
                     /gene="carB"
                     /locus_tag="VFR02_07310"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9870285.1"
                     /translation="MPKRTDIKKIMVIGAGPIIIGQACEFDYSGTQGCKALKEEGFEV
                     ILINSNPATIMTDPEFAHRTYVEPITPEVCRKIIEKERPDALLPTLGGQTALNTAVAL
                     AENGTLNEFKVELIGANLAAIKKAEDRQLFKEAMLKIGLDLPKSLTVKTLDEGLGFAE
                     GNGYPVIIRPAFTLGGTGGGIAYNRQEFEEILSKGLDLSPIHQCLVEESALGWKEYEM
                     EVMRDLNDNVVIICSIENMDPMGVHTGDSVTVAPAQTLTDKEYQRMRDASIACIREIG
                     VDTGGSNIQFGVDPRTGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYSLDE
                     IFNDITRTTKAAFEPSIDYVVTKIPRFTFEKFPDAEDILNTSMKSVGETMAIGRTFKE
                     SYQKALRSMETGTIGYDADGREFKEGRW"
BASE COUNT          360 a          513 c          478 g          276 t
ORIGIN      
        1 ggtggtcaca aatcgtgacc acctcaatgg gctaaagttt tcccactcct tgccttacgc
       61 cttcacggaa tatggggcca ttatggccgc gaatgtcttg aacagtctcg aggccgtcga
      121 gatgagcgtt ttcgtcgtac gggcttttgt gcggatgcgg gaaaaattga cggcgaacca
      181 aattttggaa aaacggttgg cggagatcga gaggaaattg ctcctgcatg acacgtccct
      241 gcgggatatt tatgacagga taaggccctt gttgctgtcg cctcccgaaa aaccgaagcg
      301 gcggattggc ttcacggccg aggagaaccg ggcccgttac ggggccggaa aaggttgaaa
      361 ggaaaatatg cctaaacgga cagacatcaa gaagatcatg gtcatcgggg ccgggcccat
      421 catcattggg caggcttgcg agttcgacta ctccggcacc cagggctgca aggccctgaa
      481 ggaagagggt ttcgaggtca tcctcatcaa ctccaacccg gccaccatca tgaccgaccc
      541 cgagttcgcc caccgcacct acgtggaacc catcacgccc gaggtctgcc gcaagatcat
      601 cgaaaaagaa cgcccggacg cgttgctgcc caccctgggc ggccagacgg ccctcaacac
      661 cgccgtggcc ttggcggaga acggcaccct caatgaattc aaggtggagc tcatcggcgc
      721 caacctggcc gccatcaaga aggccgagga ccgccaactt ttcaaggaag ccatgctcaa
      781 gatcgggctg gacctgccca agtccctgac cgtcaagacg ttggacgagg ggctgggttt
      841 cgccgagggg aacggctatc cggtcatcat ccggcccgcc ttcaccctgg gcggcacggg
      901 cgggggcatc gcctacaacc ggcaggaatt cgaggagatc ctttccaagg gcctggatct
      961 ttcccccatc caccagtgcc tggttgagga atcggccctg ggatggaaag agtacgagat
     1021 ggaggtcatg cgcgacctca acgacaacgt ggtcatcatc tgttccatcg agaacatgga
     1081 ccccatgggc gtccacacgg gcgactcggt caccgtggcc ccggcccaga ccttgaccga
     1141 caaggaatac cagcgcatgc gcgacgcctc catcgcctgc atccgggaga tcggcgtgga
     1201 cacgggcggc tccaacatcc agttcggggt cgacccccgc acgggccgca tggtggtcat
     1261 cgagatgaac ccccgggtgt cccgttcctc ggccttggcc tccaaggcca cggggttccc
     1321 catcgccaag atcgcggcca agctggccgt tggctacagc ctggacgaga tcttcaacga
     1381 catcacccgc accaccaagg cggccttcga gccttccatc gactacgtgg tgaccaagat
     1441 cccccggttc accttcgaga aattccccga cgccgaggac atcctcaaca cctccatgaa
     1501 gagcgtgggg gagaccatgg ccatcggccg caccttcaag gaaagctacc agaaggccct
     1561 gcggtccatg gagaccggca ccatcggcta cgacgccgac ggccgggaat tcaaggaagg
     1621 ccgctgg
//
LOCUS       DASTJJ010000598         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_327828, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000598 DASTJJ010000000
VERSION     DASTJJ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327828"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="VFR02_07315"
     CDS             <1..1983
                     /locus_tag="VFR02_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET9870286.1"
                     /translation="LSLQQAVISIEDERFYEHGGIDLIGIVRSFITNVMHGGVREGAS
                     TITMQLARNLFLTPERTLSRKVREALLSLQIEKNYSKQQILEMYLNQIYFGEGAYGCE
                     SAARTYFGKHVEDLTVPECALLAALPKAPNTYDPYKNPYDALKRRNLVLNSMADNNYI
                     TLQEATEYKNTPIELKKIEMQNAPYFVEFVRQQLEARYGSNAVYKGGLSVYTTLDMNL
                     QEAAQQAMENGLENAEALARKNRQSSIPVTESIQGALFAMDPHTGAIRAMIGGRNFRQ
                     SVFNRSYQALRQPGSAFKPIIYAAAIENGYTMADVILDSPVVYPDPTTGKPWRPTNFS
                     NRFRGPTTLHTALMHSINVVTVKLLDKLGIQPVVNMARRLGITTPIKPNLSLGLGTSE
                     VTLEEMVQAFSVFDNQGIRVEPFAVLSVKDSSGRVLENNTPVAQEVLDPDTAYIMTHT
                     LQDVVDRGTGRLIRRMGFTYPAAGKTGTTNDNVDAWFIGYTPDMACGVWVGYDDRISL
                     GRRQTGGETAAPIWADFMKAAVAGKPVKDFPMPTGSEDDFVTKKICTESGLLAGPYCL
                     DTRDEVFKKETAPTKYCNIHTGPTATLSGTSETDNGSSPTASGSGDILEMEAGQQAPL
                     PTPSPDASAAATATPGTAPDASATPTGGEAYPDEGF"
     gene            complement(2082..2555)
                     /locus_tag="VFR02_07320"
     CDS             complement(2082..2555)
                     /locus_tag="VFR02_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9870287.1"
                     /translation="MAIQAAILTVGDRYLSGEAKDESGSALEGICNKLGWELVKHETV
                     PDQDDHIVHWLVTNCDSGQLDVILTVDGIGIKPKDRVPESMYQVCEKWLPGFGELSRH
                     QAYANSPLVALTRGLAGVRGKTLVINLPGTPTAVKDSMDVLKPVLRHAVDQIKNP"
BASE COUNT          489 a          925 c          772 g          418 t
ORIGIN      
        1 ctgtcgctgc aacaggccgt catttccatc gaggatgagc gcttctacga gcacggggga
       61 atcgacctga tcggcatcgt ccgttccttc atcaccaacg tcatgcacgg gggggtgcgg
      121 gaaggggcca gcaccatcac catgcagctg gcccggaacc tctttttgac ccccgagcgt
      181 accctgagcc ggaaggtccg ggaggccctg ctgtcgctcc agatcgagaa gaactattcc
      241 aagcaacaga tcctggagat gtacctgaac cagatctact tcggcgaggg cgcctacggc
      301 tgcgaatccg ccgcccgtac ttatttcggc aagcacgtgg aggacctcac cgtgcccgaa
      361 tgcgccctgc tggcggccct gcccaaggcc cccaacacct acgaccccta caagaacccc
      421 tacgacgccc tcaaacgccg caacctggtg ctgaattcca tggcggacaa caactacatc
      481 accctgcagg aggccaccga atacaagaac acgcccatcg aactcaagaa gatcgagatg
      541 cagaacgccc cctatttcgt ggagttcgtg cgccagcagt tggaggcccg ctacggctcc
      601 aacgccgttt ataagggcgg cctgtcggtc tacaccaccc tggacatgaa cctgcaggag
      661 gccgcccagc aggccatgga gaacgggctg gagaacgccg aggccctggc ccgcaagaac
      721 cgccagagca gcatccccgt gaccgaatcc atccagggcg ccctcttcgc catggacccc
      781 cacaccgggg ccatccgcgc catgatcggg gggcgcaatt tccgccagag cgtcttcaac
      841 cgctcctacc aggccctgcg ccaacccggg tccgccttca agcccatcat ctacgcggcg
      901 gccatcgaga acggctacac catggcggac gtcatcctgg attcgccggt ggtctatccg
      961 gacccaacga ccggcaagcc ctggcgcccc acgaattttt ccaaccggtt ccgggggccc
     1021 accaccctgc acaccgcgct catgcactcg atcaacgtgg tgaccgtcaa gctgctggat
     1081 aagctgggga tccagcccgt ggtcaacatg gcgcggcgcc tgggcatcac cacccccatc
     1141 aagcccaacc tgtccctggg gctgggcacc tccgaggtca cgttggagga gatggtgcag
     1201 gccttttcgg tgttcgacaa ccagggcatc cgggtggagc ccttcgccgt cctttcggtc
     1261 aaggactcgt cgggacgggt cctggagaac aacacccccg tggcccagga ggtcctggac
     1321 cccgacaccg cctacatcat gacccacacg ctccaggacg tggtggaccg cggcacgggg
     1381 cgcctcatcc gccgcatggg cttcacctat cccgccgcgg gcaagaccgg gaccaccaac
     1441 gacaacgtgg acgcctggtt catcggctac acgcccgaca tggcctgcgg ggtctgggtg
     1501 ggctatgacg accgcatcag cctgggccgc cggcagaccg gcggcgagac cgccgccccg
     1561 atctgggccg acttcatgaa ggccgccgtc gcgggcaaac ccgtgaagga cttccccatg
     1621 cccaccgggt ccgaggacga cttcgtgacc aagaagatct gcaccgaatc cgggctgctg
     1681 gccggcccct attgcctgga tacccgcgat gaggtcttca agaaggaaac ggcgcccacc
     1741 aagtactgca acatccacac cggccccacc gccacccttt cggggacctc cgaaacggac
     1801 aacggttcat cccccaccgc ctcgggatcc ggcgacatcc tggagatgga agcgggccag
     1861 caggcgcccc tgcccacgcc ctcccccgac gcctccgcgg cggccaccgc cacgccgggg
     1921 accgcgcccg acgcctccgc cacgcccacc ggcggcgaag cctatccgga cgagggattc
     1981 tgacccttcg cgcgggacgc catggcggtc ccggggcctg aaagcaaaaa agccccgccc
     2041 ccttcggggg gcggggcttt ttttatccgg gggaaggccc ttcaggggtt cttgatctgg
     2101 tccaccgcgt ggcgaaggac gggtttaagg acgtccatgg agtccttgac cgccgtgggg
     2161 gtgccgggca ggttgatgac gagggtcttg ccccgcaccc cggccaagcc ccgggtcaag
     2221 gccaccaggg gggagttggc gtaggcctgg tgccgggaaa gttcgccgaa acccggcagc
     2281 catttttcgc acacctggta catggactcg gggacccggt ccttgggctt gatgccgatg
     2341 ccgtccaccg tcaggatgac gtccaattgc cccgaatcac agttggtgac cagccaatgc
     2401 acgatgtggt cgtcctggtc cgggacggtc tcatgcttga ccagttccca tcccagcttg
     2461 ttgcagatgc cttccagggc cgaaccgctc tcatccttgg cctcgccgga gaggtagcgg
     2521 tccccgaccg tcaatatcgc cgcttgaatg gccatggggc ctcccgttac ttcttgttgt
     2581 ccaggtccag gttgcccagg tcgc
//
LOCUS       DASTJJ010000599         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_206379, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000599 DASTJJ010000000
VERSION     DASTJJ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206379"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFR02_07325"
     CDS             <1..742
                     /locus_tag="VFR02_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870288.1"
                     /translation="AGGSGAVNVANTGFTATANWMASSCAVDPSHKANQVILFTPNGS
                     HQTIPSNGGYWGGGFNLGIGRNNPQMDDAWADDYSKTSVCSGGLTEDSHFALYYDGTL
                     LLEWSENAVQDTQSGAEPCCGGTVHASMISKQDIVLGGPTQTPTTAVGTDAWSVTAAP
                     NMTDGRQPVQFQVRLDRPEKITLKLYTLLGEKICEDQVMGTAGPNILIWRAQNEAKQS
                     VASGIYLYAVQIEGQTGMFKSGKVLVLH"
     gene            859..>2706
                     /locus_tag="VFR02_07330"
     CDS             859..>2706
                     /locus_tag="VFR02_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9870289.1"
                     /translation="MSFLPSANLKRSLALLALAWGLGAGAWAQTSPATITVDGATTLT
                     SFAPISVFGNNTAYWISNTANQAVRAKVEAAGNYFLRYPGGSSSDDYHWNGTGSYDAN
                     GYWVPSGTSYTYGFVGNETYRGTTSSYGTPSHITDNDNSTSWFSNVNTDFPSHQWAEL
                     DLATPSTVNAVTVVWGSPYATSFQIQYWKYNGGYPLPYGNSPENSWTTTSAGTVVGTG
                     GTQGITFTAPSAAQYWRILMTASSAGVSGAYSIDEVKLYNGSTQVSSNSVTSTVQTQV
                     EVSSTDPASTFSYPTSPSGFTDFESFMTYANAFSPHAIPLITVNLGTGTPSEAASWVY
                     YANVVKGYGIKYWQIGNETEGAWETGGPLNAEDYVRRYVQYFDAMKAVDPSIVITGPV
                     SGSFTDSSNLFDGKSYVQDFISLLHGMGKDSYLNAIDFHWYPNYGNYTPAAALASPAQ
                     LDSFPVSLSSWLSGVPGGSALPVIMSEYNVDAGDENFQVQLGNGLWVADSLGHFITDF
                     GSRGFSNLWDTLNGGSGTTSTTGGDLGYLNVKDDLPSHLYQYQAHASYWAMQMMAGDW
                     AIPGDSAVHHLVSTTVAGAPSSLLSAYSDYRPDGILSLVVVNKNPTTSYA"
BASE COUNT          557 a          810 c          775 g          564 t
ORIGIN      
        1 ggcgggagga tcgggcgcgg tcaacgtcgc caacacgggg ttcacagcca ccgccaattg
       61 gatggcttca tcctgcgcgg tggatccgtc ccacaaagcg aaccaagtca ttctctttac
      121 gccaaatggg agtcaccaaa caatcccctc gaacggtggt tattggggtg gtgggttcaa
      181 cttggggatt ggacggaata acccgcagat ggacgatgct tgggcggatg attacagtaa
      241 aacatccgtt tgctcgggtg ggttgacgga agacagtcat ttcgcgctct attatgatgg
      301 gacccttttg ttggagtgga gcgagaacgc tgtccaagac acccaaagtg gggcagagcc
      361 ttgttgcggc gggacggtcc atgcttcaat gatctcaaag caagacatcg ttctcggagg
      421 gccgacccag acccccacga cggccgtggg gacggatgct tggagcgtga cggccgcgcc
      481 caatatgacc gacggccgac aaccggtcca attccaggtg cggctggaca ggccggaaaa
      541 aatcacgctg aagttgtaca ccttgttggg cgaaaagatt tgtgaagacc aggtcatggg
      601 aacggcgggg ccgaacatct tgatttggcg ggcccaaaat gaagcgaaac aatcggtcgc
      661 cagtgggata tatctgtatg ctgtgcaaat agagggtcag acaggtatgt tcaaatcggg
      721 gaaagttttg gtcctacatt aagggttcaa cattaattct tcccaagcgg ctttgatcct
      781 ctgttcgctt atccctcgcg agggacttgt ttttggtatg attaccctac aggccgattc
      841 aacctgaagg ggttcttttt gtcattttta ccttccgcca acttgaaacg atccctggcc
      901 ctcctggccc tggcctgggg gctgggcgcc ggcgcttggg cccagacctc gcccgccacc
      961 atcacggtgg acggggccac gaccctgacc tccttcgcgc ccatcagcgt cttcggcaat
     1021 aacacggcct attggatctc caacacggcg aaccaggccg tgcgggccaa ggtggaggct
     1081 gcggggaact atttcctgcg ttatccgggt ggctcctcct cggacgacta ccattggaac
     1141 ggcaccggct cctacgacgc caacggttac tgggtgccaa gcgggacgtc atatacctat
     1201 ggtttcgtgg gcaacgagac ctaccggggc actacctcca gctatggaac cccctcccat
     1261 atcacggaca acgacaattc cacatcttgg ttttccaacg tcaacacgga tttccctagc
     1321 catcaatggg ctgaattgga cttggccacc ccttccactg tgaatgccgt gaccgttgtt
     1381 tgggggagtc cctacgcgac ctcctttcag atccaatact ggaaatataa cggcggctac
     1441 cccttgcctt atggaaatag tccggaaaac agctggacga cgaccagtgc tggtacggtg
     1501 gtggggaccg gagggaccca ggggattact tttaccgccc cgtcggccgc ccaatattgg
     1561 cggatattga tgaccgccag ctcggcgggg gtgagtgggg cctattccat cgacgaggtg
     1621 aagctctaca acgggagcac ccaggtctct tccaatagcg tcacctcgac agtccaaacc
     1681 caagttgagg tttccagcac ggacccggcc agcactttta gctatcccac cagcccgtca
     1741 ggcttcacgg acttcgagtc cttcatgacc tacgccaacg ctttcagccc ccatgccatt
     1801 cccctgatca cggttaattt agggacgggc acgccctcgg aggcggcctc ctgggtctat
     1861 tacgccaacg tggtgaaggg ctatgggatc aagtactggc agatcggcaa cgagaccgag
     1921 ggcgcctggg agaccggtgg ccctttgaac gccgaggact acgtacgacg ctacgtccag
     1981 tactttgatg ccatgaaggc ggtggatccc tccatcgtca ttacgggccc cgtttcgggc
     2041 agcttcaccg acagctcgaa tctttttgat gggaaaagct acgtgcagga cttcatcagc
     2101 ctgctgcacg gcatgggcaa ggacagctac ctcaacgcca tcgacttcca ttggtacccc
     2161 aattacggca actacacccc ggcggccgcc ctggccagcc ccgcccaatt ggattccttc
     2221 cccgtgagcc tgagctcttg gttgtctgga gtcccgggcg gctccgccct gcccgtcatc
     2281 atgagcgagt acaacgtgga cgcgggtgac gagaatttcc aggtgcagtt gggcaacggc
     2341 ctctgggtgg ccgattccct gggccatttc atcaccgact tcgggagccg tggtttttca
     2401 aacctgtggg acaccttgaa cggcggcagc ggcaccacca gcaccaccgg cggggacctg
     2461 ggctacctga atgtgaaaga tgaccttccg tcccatcttt accaatacca ggcccacgct
     2521 tcctattggg ccatgcagat gatggccggc gattgggcca tcccgggcga ctccgcggtc
     2581 caccacctgg tgtccaccac ggtggcgggg gcgccctcgt cgctgctctc ggcttattcg
     2641 gactaccgcc ccgacggcat cctgtcgctg gtggtggtga acaagaaccc cacgaccagc
     2701 tacgcc
//
LOCUS       DASTJJ010000600         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1428252, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000600 DASTJJ010000000
VERSION     DASTJJ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428252"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFR02_07335"
     CDS             complement(<1..622)
                     /locus_tag="VFR02_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870290.1"
                     /translation="MTSKARSNPTFKFPAILLAGLVLLFPALSRAAAINYTSTSYCPT
                     YTTFTVSEQTTAYDLNTSSPTTLGNCGVNEGMFDPNHYAAIEPGDWQNGAACGACAVL
                     NYGANATTVMIVDKCATCGTGSHHLDLSPKAYGELLGSPNCASPTGSGGGVNTGGCPG
                     APGGGSVNWHFIQCPLTGSAAITYNNSSGNITYAFKDSSSLGGWEPI"
     gene            complement(995..>1190)
                     /locus_tag="VFR02_07340"
     CDS             complement(995..>1190)
                     /locus_tag="VFR02_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9870291.1"
                     /translation="GMRVYNSAGEFIKNLAPGTDPQGLSHSFLWDGTNYLNQPCADGI
                     YILYYLQPRQVSETKVLLLR"
BASE COUNT          231 a          292 c          403 g          264 t
ORIGIN      
        1 tgatgggctc ccaccccccg aggctgctgc tgtccttgaa ggcgtaggtg atgttgccgc
       61 tcgaattgtt gtaggtgatg gccgccgaac ccgtgagggg acattggatg aaatgccagt
      121 tcaccgagcc gccccccggc gcccctggac aaccgccggt gttgaccccg ccgccgcttc
      181 cggtcggact ggcgcaattg gggcttccca gcaattcccc gtaggctttg ggcgagaggt
      241 ccaaatggtg ggaaccggtg ccgcaggtgg cgcacttgtc gacgatcatg acggtggtgg
      301 cgttggcccc gtagttcaag accgcgcagg cgccgcaggc ggcgccgttc tgccagtcgc
      361 ccggctcaat ggccgcgtaa tggttggggt cgaacatccc ctcgttgacg ccgcaattgc
      421 ccagggtggt ggggctggaa gtgttgaggt cataagcggt cgtttgttcg gagaccgtga
      481 aggtggtgta ggtggggcag taggaagtgg aggtgtaatt gatcgcggcg gcccgggaaa
      541 gggcggggaa caaaaggacc aggcccgcca agaggatggc agggaacttg aaggttggat
      601 tggatcgggc cttcgaggtc aatcgaggac tcctggtagt ttggtggccc agggccggag
      661 cttaaatcgt tattaagcta acggcgcttg ggaaaaagtt aagcaattcc aatagtttat
      721 catcgctggg cgtggatggg tgtcgggtcg cgtttccata tataagggca atttccatgc
      781 cctggaaggg ttatttattt ttatcgtgaa tatggtggcc aggccttatt tttgagcgcc
      841 cggagttgaa ccgattaaac tgttggaggt attcaatttg tcaacttttt cgactttttt
      901 gtaataactc tgacgtcccg cttcccaagg gtcgggcggc aaggatgccc ttggctgagg
      961 ggaaccacga agcccgctcc atcatccaaa agggtcaacg caacaagagg accttggtct
     1021 cgctgacttg gcggggttgg aggtaataga ggatatagat gccgtcggcg cagggttggt
     1081 tcaggtagtt ggtgccgtcc caaaggaagg aatgggacag gccctggggg tcggtccccg
     1141 gcgccaggtt cttgatgaac tccccggcgg aattgtagac ccgcatcccg
//
LOCUS       DASTJJ010000601         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_571592, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000601 DASTJJ010000000
VERSION     DASTJJ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571592"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="VFR02_07345"
     CDS             <1..>1113
                     /locus_tag="VFR02_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9870292.1"
                     /translation="AKIKETEAKLLQALSTASPLQVSQVRSAAIKTMAEELKTTLGII
                     KGYVSSALGATQGGINEKQQEFLGMVINRSARLEKFINDLLDIYQVEIEQADAKTEEV
                     NLPAEIEGLAFNFQAQAEVKNIKIKVEGKPGLPKVPIVRRRFNQLWNILYLQIIKDAP
                     RGSTISISLEPLGDMVKVSVVDPGLVVKPESLPKLFDEFYDPKHPASTQLAGTGLKFA
                     LIKTILAAHGGGAVAEASNPGTTLILNFPTKRKAPGAAAPVPAPTAAPAAAPAAPAPG
                     VAPTPAAKPAFTVPGVAPKTASPMAPGLAPKPPAAAPGVPPAGAPPAAKPGPVPGVLD
                     SLLAGKVPPAAPKPAAPGPAALDSLLAGNVPPVTAAP"
BASE COUNT          183 a          430 c          346 g          154 t
ORIGIN      
        1 ggccaagatc aaggagaccg aggccaagct gctgcaggcc ctgtccaccg cctcgcccct
       61 gcaggtgtcc caggtgcggt ccgccgccat caagaccatg gccgaggagc tcaagacgac
      121 gcttggcatc atcaagggct acgtgtcctc cgcgttgggc gccacccagg gcggcatcaa
      181 cgagaagcag caggaattcc tgggcatggt catcaaccgc agcgcccgcc tggagaagtt
      241 catcaacgac ctgctggaca tctaccaggt cgagatcgaa caggccgacg ccaagaccga
      301 ggaggtcaac ctgcccgccg agatcgaggg cttggccttc aacttccagg cccaggccga
      361 ggtgaagaac atcaagatca aggtggaagg gaagccgggc ctgcccaagg tgcccatcgt
      421 gcggcgccgc ttcaaccagt tgtggaacat cctgtacctg cagatcatca aggacgcgcc
      481 ccgcggctcc accatctcca tctcgctgga gcccttgggg gacatggtca aggtctcggt
      541 ggtcgacccc ggattggtgg tgaagccgga aagcctgccc aagcttttcg acgagttcta
      601 cgaccccaag cacccggcct ccacccagtt ggcgggcacg ggcctcaagt tcgcgctcat
      661 caagaccatc ctggccgccc acggcggcgg cgcggtggcg gaggcctcca accccggcac
      721 cacgctcatc ttgaatttcc cgaccaagcg taaggccccg ggcgcggcgg cccccgtccc
      781 ggcgcccacg gccgctcccg ccgccgcccc ggcggctccc gcgccgggcg tggcgccgac
      841 ccccgccgcc aaaccggcct ttacggtccc cggcgtggcc cctaaaaccg cttcccccat
      901 ggcgcccggc ctggcgccca agcctcccgc ggcggcgccc ggggttcccc ccgcgggagc
      961 cccgcccgcg gccaagccgg gtcccgtccc cggcgtgttg gattcgcttt tggccggcaa
     1021 ggtcccgccc gccgcgccga aaccggcggc gcccggtccc gccgccttgg attcgctcct
     1081 ggccggcaat gtgccgcccg tgacggccgc ccc
//
LOCUS       DASTJJ010000602         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1428411, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000602 DASTJJ010000000
VERSION     DASTJJ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428411"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /gene="topA"
                     /locus_tag="VFR02_07350"
     CDS             complement(<1..1903)
                     /gene="topA"
                     /locus_tag="VFR02_07350"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9870293.1"
                     /translation="MPKAGARKKKENTKKLVIVESPSKAKTINKYLGSDFVVLASKGH
                     LIDLPKSKLGVAVDNNFEPQYIPIRGKASIIKELKKAALKATTVYLATDPDREGEAIG
                     WHIKNFLSALENKPPLYRLRLNEITKNAIEEAIQTPSEVDLNMVNAQQARRVLDRLVG
                     YGISPLLWKNIRRGLSAGRVQSVALRLIYEREKEIEGFKPVEYWTLEADFLTADGRLI
                     RTKLSKLGGEKPDLKTQEETQALIERLKAASFQVSAVLHREKSKKPPMPFTTSKLQQE
                     AYKQLRFGARKTMSIAQGLYEGVEVGESGPVGLITYMRTDSKRISPEAKAEAAKYIRE
                     AYGDKYLSPGDRDTSSKTKIQDAHEAIRPTSVFRTPDQMKNFLTPEQFKLYQLIWEDF
                     LASQMSNALYRETSVEISGADAVFRASGTETLFEGYTKVYEEAADEDKKEGEGEGDEK
                     GMNPNKLSGIEEGQPMTWKEPVPEQHFTQPPPRYTDASMVKTLEELGIGRPSTYAPTL
                     ETIQTRHYVKKENGRFFLAELGNLVLEILMKNFPNILDYTFTAKMETELDQVEAGEQE
                     WRHVISDFYTPFKDTLAEAEKLIGEEKSKIEQVTDIPCEKCGQMMIVKWGRHGKFLAC
                     SKYPDCQNTK"
BASE COUNT          291 a          623 c          671 g          468 t
ORIGIN      
        1 tcttggtgtt ctggcagtcc ggatacttgg agcaggccag gaacttgccg tggcggcccc
       61 acttgacgat catcatctgg ccgcacttct cgcaggggat gtcggtgacc tgctcgatct
      121 tgctcttttc ctcgccgatg agcttctcgg cctcggccag ggtgtccttg aagggcgtgt
      181 agaagtcgct gatgacgtgg cgccattcct gctcgccggc ctccacctgg tccagctcgg
      241 tctccatctt ggccgtgaag gtgtagtcca ggatgttggg gaagttcttc atgaggatct
      301 ccaacaccag gttgccgagc tcggccagga agaaacggcc gttctccttc ttcacgtaat
      361 gccgggtctg gatggtctcc agggtgggcg cgtaggtgga ggggcgcccg atgcccagct
      421 cttccagcgt cttgaccatg gaggcgtccg tataacgggg cggcggctgg gtgaagtgct
      481 gctcgggcac cggctccttc caggtcatgg gctggccttc ctcgatgccg gagagcttgt
      541 tggggttcat gcccttctca tccccttcgc cttccccttc cttcttgtcc tcgtcggcgg
      601 cctcttcata gaccttggta tagccctcga agagggtctc ggtgcccgag gcccggaaaa
      661 cggcgtccgc gcccgagatc tccacgctgg tctcccggta gagggcgttg ctcatctggc
      721 tggccaggaa gtcctcccag atcagctggt agagcttgaa ctgctcgggg gtcaggaagt
      781 tcttcatttg gtcgggggtg cggaagaccg aggtgggccg gatggcctcg tgggcgtcct
      841 ggatcttggt cttgctggag gtgtcccggt cccccgggct caggtacttg tcgccgtagg
      901 cctcccgaat gtacttggcc gcctcggcct tggcctcggg ggagatgcgt ttggagtccg
      961 tcctcatata agtgatcaag cccacggggc ccgattcccc gacctccacg ccctcataga
     1021 ggccctgggc gatggacatg gtcttgcggg cgccgaaacg cagctgttta taggcctcct
     1081 gctgcagctt ggaggtggtg aagggcatgg ggggtttctt ggacttttcc cggtgcagca
     1141 cggccgacac ttggaacgag gcggccttca ggcgctcgat cagggcctgg gtctcctcct
     1201 gggtcttcag gtcgggtttt tccccgccga gcttggagag cttggtgcgg atgagccgcc
     1261 cgtcggccgt gaggaagtcg gcctccaggg tccaatattc caccggcttg aacccctcga
     1321 tctccttttc ccgctcatag atgagccgca gcgccaccga ctggacgcgc cccgccgaca
     1381 ggccccgccg gatgttcttc cacaacagcg ggctgatgcc gtaacccacc aaccggtcca
     1441 acacccgccg ggcctgttgg gcgttgacca tgttcaggtc cacctcgctg ggggtttgga
     1501 tggcctcctc gatggcgttc ttggtgatct cattgaggcg caggcggtag aggggcggct
     1561 tgttctccag ggcgctcagg aaattcttga tgtgccagcc gatggcctcc ccttcccggt
     1621 ccgggtccgt ggccaggtag acggtggtgg ccttcagggc ggccttcttg agttccttga
     1681 tgatggaggc cttgccccgg atggggatgt actggggctc gaagttgttg tcgacggcca
     1741 cgcccagctt gctcttgggc aggtcgatca ggtgtccctt ggaggccagc accacgaaat
     1801 ccgagcccag gtacttgttg atcgtcttgg ccttggacgg cgactccacg atgaccaact
     1861 tcttggtgtt ttccttcttt ttcctggctc cggcttttgg cacgagaggc ccccttggcg
     1921 gattcggcgg cgaacgccgc aatttcgcaa gaatagggag ggggcccttg cttgtcaagt
     1981 tttcaagttc cggacggcgg gggggatccg ccgtccacgc gggcccgggg acgcccggcg
     2041 cgccctgaaa acg
//
LOCUS       DASTJJ010000603         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1306351, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000603 DASTJJ010000000
VERSION     DASTJJ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306351"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFR02_07355"
     CDS             <1..1002
                     /locus_tag="VFR02_07355"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HET9870294.1"
                     /translation="GNKVFVNILLLGAAFQRGLLPLTLEEMLQAIRESFNPSAAKHNL
                     DAFHLGRDLVLNPGKYGAEVKPGLEEVVRKKEALLGGRRGRAYRAMLSEFLGAWTGDE
                     ETRLQFASRLYDLIEFEDPAYARGYLDRVMAVLRKDSPAGQFAATQAVVKYLHKVMEI
                     KDEVYVAHLLTSPEKYEKDARRYKMDLAAGDRITYRHFNRPEFDLWGLKIVFDLDSRD
                     WMLRGMKRLKFLRRLLPSWHAREKAFRAWYQSLVDRFEFTGPEQYDRWVRALSVPDEV
                     RGYRAIRYPKMERAQDLADQILSGREIPQAIRRPRPASSFPRFDPLEMTGFRPILRG"
     gene            1141..1341
                     /locus_tag="VFR02_07360"
     CDS             1141..1341
                     /locus_tag="VFR02_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9870295.1"
                     /translation="MAQGTVKWFKNEKGFGFISQDGGEDVFVHFSAIQSDGFKSLNEG
                     DRVEFDVARGDKGLKAENVRKI"
     gene            1760..>2154
                     /locus_tag="VFR02_07365"
     CDS             1760..>2154
                     /locus_tag="VFR02_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870296.1"
                     /translation="MNPHDHGSQRFRKQAPAPGLPAFWRDLVRGRKRLYASSLAFKTL
                     LALVPALVLCMTLLSSDRFLEQRERLLDRLVGLVYPVDDSALDAGERRSIQRLNRTAK
                     EGIRASIHRFAAHSAKVGMAGLGAFFLLL"
BASE COUNT          391 a          682 c          640 g          441 t
ORIGIN      
        1 ggcaacaagg tgttcgtcaa tatcctgctg ttgggcgcgg ccttccagcg gggcctgctg
       61 cccctgaccc tggaggagat gttgcaggcc atccgggaaa gcttcaaccc cagcgcggcc
      121 aagcacaacc tggacgcctt ccacctgggc cgggacctgg tcctgaaccc cggcaagtac
      181 ggcgccgagg tgaagccggg cctggaggag gtcgtccgca agaaggaggc cctcctgggc
      241 ggccgccgcg gccgggccta tcgggccatg ttgtccgagt tcctgggcgc ctggacgggc
      301 gacgaggaga cccggctgca gttcgcttcc cggctctatg acctcatcga gttcgaggac
      361 ccggcctacg cgcgcggcta cctggaccgg gtgatggcgg tgctgcgcaa ggacagcccg
      421 gcgggccagt tcgcggccac ccaggccgtg gtcaagtacc tgcacaaggt catggagatc
      481 aaggacgagg tctacgtggc ccacctgctc accagccccg aaaaatacga gaaggacgcc
      541 cggcgctaca agatggacct ggcggcgggg gaccgcatca cctaccggca cttcaaccgc
      601 cccgagttcg acctctgggg cctcaagatc gtcttcgacc tcgacagccg ggactggatg
      661 ctgcggggca tgaagcggct caagttcctg cggcgcctgc tgccttcctg gcacgcgcgg
      721 gagaaggcct tccgggcctg gtaccagtcg ctggtcgacc gcttcgagtt caccggcccc
      781 gagcagtatg accgctgggt gcgcgcccta tccgtgcccg acgaggtccg gggctaccgg
      841 gccatccgct accccaagat ggaacgggcc caggacctgg ccgaccagat cctctcgggc
      901 cgggagatcc cccaggccat ccggcgcccc cggccggcaa gcagctttcc aaggtttgac
      961 ccccttgaaa tgacgggctt tcgccctatc ctgagggggt agctgcgtcg gtcttgccgg
     1021 aaatttgttg attaatttct tcccctttcc cccaattcct gtatgatggc tgcgtccagt
     1081 tgggcccgac gggttctctt ggaagatttt aaatttttag aagagtagag gtttcataga
     1141 atggcacaag gtactgtgaa gtggttcaag aatgagaagg ggtttggctt catctcccag
     1201 gacggtggcg aggatgtgtt cgtccacttc tcggccatcc agtcggatgg attcaagagc
     1261 ctgaacgaag gtgaccgcgt tgagttcgac gtcgctcgcg gtgacaaggg cttgaaagcc
     1321 gaaaacgtcc gcaagatcta actcaacctt aggttttgca gtaaaacccc cgggccctgc
     1381 ccgggggttt tttttgggga tcacgcactt ccaacgcaaa agcaattgga acattgataa
     1441 agactggtaa aacctgatct ttagaggctc caacaaaatt cgatcagcca tgatgaacgc
     1501 cgatgaaagc tgtaaaaaac gatggaaatc gcttcatcct gcgttcatcg gcgtccatcc
     1561 atgctggcat tgccttgggt tttgttacgg cctcttattt tcaaacatcg gatttcatca
     1621 gtttttatca ttgttctaag ttctcttttg cttgttttgt aaaagccata gtcccgtttt
     1681 ttttgcccct gcctttcccg ttgcatttcg ccccatcccc cgttaatttc atgccaaaac
     1741 cggcccgccc ggaggggact tgaacccgca cgaccacggc tcccaacgtt tccgaaagca
     1801 agcgcccgct ccgggcctgc ccgccttctg gcgggacctg gtccggggcc gcaagcgcct
     1861 ctacgccagc tcgctggcct tcaagaccct gctggccctg gtgcccgccc tggtgctctg
     1921 catgaccctg ctgtcctccg accggttcct ggagcagcgg gaaaggctgt tggaccgcct
     1981 ggtgggcctg gtctacccgg tggacgactc ggccctggac gccggggaac gccgcagcat
     2041 ccagcgcctc aaccggaccg ccaaggaggg catccgcgcc tccatccacc gcttcgccgc
     2101 ccattcggcc aaggtgggga tggcgggcct gggcgccttc ttcctgctgc tctt
//
LOCUS       DASTJJ010000604         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_694586, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000604 DASTJJ010000000
VERSION     DASTJJ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694586"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFR02_07370"
     CDS             complement(<1..823)
                     /locus_tag="VFR02_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9870297.1"
                     /translation="MDLFDSVPEDKDAVRPLAIRMSPKTLDEYVGQEHLVGPGKLLRR
                     LLESGRVVSLILYGPPGTGKTGFARLVARKLQGRFVALNAVTSGLAELRQVFQEAVET
                     KKLYQKATVLFLDEIHHFNKSQQDALLPHLEQGTIILVGATTQNPFFALNSALLSRSR
                     VCELKPLSEKDLDAILDRALADPDRGLGRQKIVLTPEARVHLVRSASGDGRVLLNALE
                     VAALTTPPDAQGVLTLDLQAAQESVQRKFVQYDASGDQHYDTISAFIKSIRGSDPD"
     gene            complement(879..1058)
                     /gene="ssrS"
                     /locus_tag="VFR02_07375"
     ncRNA           complement(879..1058)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFR02_07375"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          209 a          377 c          468 g          245 t
ORIGIN      
        1 cgtccgggtc cgaaccccgg atgctcttga tgaaggccga aatggtgtcg taatgctggt
       61 cgccggaagc gtcgtattgg acgaacttgc gctgcacgga ttcctgcgcc gcttggaggt
      121 ccagggtgag gaccccctgg gcgtcgggcg gtgtggtgag ggcggccacc tccagcgcgt
      181 tcaagagcac ccggccgtcc ccggaggccg accggaccag gtggacccgc gcctcggggg
      241 tcaggacgat cttttgccgg cccaggcccc ggtcggggtc ggccagggcc cggtccagga
      301 tcgcgtccag gtccttctcg gacaggggtt tgagttcgca cacgcgggag cgggacagga
      361 gggcgctgtt gagcgcgaag aaggggttct gggtggtggc gcccaccagg atgatggtgc
      421 cctgttccaa gtgggggagc agggcgtcct gctggctctt gttgaaatgg tggatctcgt
      481 cgaggaagag gacggtggcc ttttggtaga gcttcttggt ctcgacggcc tcctggaaga
      541 cctgccgcaa ctcggcgagc cccgacgtga cggcgttgag ggccacgaaa cgcccttgca
      601 gcttgcgcgc caccaggcgg gcgaagcccg tcttgcccgt gcccgggggg ccgtagagga
      661 tgagggagac cacgcggccc gattccagca accggcgcag cagcttgccc gggcccacca
      721 ggtgttcctg gcccacatat tcatccaggg tcttggggga catgcggatg gccagcggcc
      781 ggacggcgtc cttgtcctcg gggaccgaat cgaacagatc catggggctc atgataggtc
      841 gggcggggac gggccacaag ggcgggggaa aaacaaaaaa gccgcccgcc tgtgccgttt
      901 gagacggtac ttttctgagg cctgactagg tcaggtgggc gcccgatcga gcggttcatc
      961 ggcttccgaa gaagactatc cccgttcggt ttagggcacc cgttttttat tttgtggctc
     1021 agaatttccc gccccaagct cgaacaccgc aggaggctct gggatcattt taagaacgcg
     1081 ggggcccttg tcaagcgcgg ccgattaagt tcaccgaaag ggttgagaat tcgggggggc
     1141 tgtatcagaa tgcatgccat cctccaggga gtcgtgtccc gtgatgagat tccaagtgca
     1201 ggcgttgcgc gcgcagggct ggtggcttcc cctcgccctg gtggcggggt tggcgctgat
     1261 cccgctggcg ctcttcgtcg gcttggcgct gctggccct
//
LOCUS       DASTJJ010000605         1431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1674222, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000605 DASTJJ010000000
VERSION     DASTJJ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674222"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFR02_07380"
     CDS             complement(<1..384)
                     /locus_tag="VFR02_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870298.1"
                     /translation="MKTPIRLALLAVLFFPLNLEAAPRTSASVAGVKGYSSLAPVHGD
                     TLLTVAVVVKVKPGWHVNAHRGLPEGFIPLTLSLAPGAPASLVDTRYPEGVKKALAGL
                     PPFPVYQGSVTLFADLRLAEKLPLGP"
     gene            complement(456..>1431)
                     /locus_tag="VFR02_07385"
     CDS             complement(456..>1431)
                     /locus_tag="VFR02_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9870299.1"
                     /translation="IGTVSRVLNNKKWVSEDCRRKVLLAIKDLHYKPQAHARRLRQKH
                     SSIWGVIAPHHTTIFGSPFFTKIMQGLEEVAAEKHYRLLFHPLNETARAQVSYRALLG
                     DGSVDGMFVLNAWSTDASIRELSDANVPFVLINGKISAQEELPYVGLDNKGGVKMAIK
                     HLAALGHERIGMINGRMTATNALERFQAFQETLAEYRLDFQNEWVADGDFEEEGGFRA
                     AQKIMSAVRRPSAILCANDLMAIGAIRALKLKGSNVPEHISVVGFDDMEEAVYHQPAL
                     TTVSFSAYQMGRLAAQKMAQIMAEETLEQKATTLQSELIVRESTGRAV"
BASE COUNT          252 a          446 c          482 g          251 t
ORIGIN      
        1 cgggcccaaa ggcagctttt ccgccaagcg caggtcggcg aacagggtga cgctcccttg
       61 atagaccgga aagggcggca ggcccgccag ggccttcttg acgccctcgg gataacgggt
      121 gtccaccagg ctggccggcg ccccgggcgc cagcgaaagc gtcaagggga tgaacccctc
      181 cggaagcccc cggtgggcgt tcacgtgcca gccgggcttg accttcacga ccaccgccac
      241 cgtcaagagc gtgtccccat ggacgggcgc cagggacgaa tagcccttca cccccgccac
      301 ggaggcgctg gtccgtgggg cggcctcaag gttcaggggg aaaaaaagga cggccagaag
      361 ggccaagcgt atgggggttt tcatatgggg agaatacccc atttatggaa aagcggttac
      421 cctccccctg aaaaaaaggg gaggcccggg acgcgtcaaa ccgcccggcc ggtggattcc
      481 cgcacgatca gctcggactg gagcgtggtg gccttctgct ccaacgtctc ctcggccatg
      541 atctgcgcca tcttctgggc cgccaatcgg cccatctgat aggccgagaa ggagaccgtc
      601 gtgagggcgg gctggtggta gaccgcctcc tccatgtcgt cgaagccgac caccgagatg
      661 tgctcgggca cattggaccc cttcaacttc agggcccgga tggcgccgat ggccatcagg
      721 tcgttggcgc agaggatggc gctggggcgg cgcacggcgg acatgatctt ttgggccgcc
      781 cggaacccgc cttcttcctc gaagtccccg tcggccaccc attcgttctg gaagtccagc
      841 cggtattcgg ccagggtctc ctggaaggcc tggaaacgct ccagggcgtt ggtggcggtc
      901 atgcggccgt tgatcatgcc gatgcgctcg tggcccaggg ccgccaggtg cttgatggcc
      961 atcttgaccc cgcccttgtt gtccagcccc acgtagggca gctcttcctg ggccgagatc
     1021 ttgccgttga tgagcacgaa aggcacgttg gcgtccgaaa gctcccggat ggaggcgtcg
     1081 gtggaccagg cgttcagcac gaacatgccg tccacgctgc cgtcgcccaa gagcgcccgg
     1141 taggagacct gggcccgggc cgtctcgttg agggggtgga acaaaagccg gtaatgcttt
     1201 tccgcggcca cttcctccag gccctgcatg atcttggtga aaaaagggct gccgaaaatg
     1261 gtggtgtggt ggggggcgat gaccccccag atgctggagt gtttctgccg caggcgccgg
     1321 gcatgggcct ggggcttgta gtgcaggtcc ttgatggcca acaagacctt gcggcggcag
     1381 tcttcgctga cccacttctt gttgttgagg acccgagaaa cggtcccgat g
//
LOCUS       DASTJJ010000606         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_940870, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000606 DASTJJ010000000
VERSION     DASTJJ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940870"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1876)
                     /locus_tag="VFR02_07390"
     CDS             complement(<1..>1876)
                     /locus_tag="VFR02_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET9870300.1"
                     /translation="CSGATLYLFLPHSGLSYTSFISIYLLAQVFGTVSQVPGGLGILE
                     TTFVVLLSPALPAPSVLGAVLAFRLVYFFIPFLLGLSALTGYEVTHNKEGFKRALQIF
                     NRWMPDLAPFGFAGLTFFAGALVLFFNANPEVTRRLLWLNEFIPLGLLEGSHLAAGVL
                     GAMLLLLARGLQQRLDIAYSLTLVCLGLGALASLLKGFDYGQALCLLALFGVLLPCRR
                     YFPRKSSLFYQRYPPLWVTAILFVWLGSIWVGVFNYRYEDYTNDLWSTFDLLEDAARF
                     LRSMLGATATLVLYAVVGLLSPDQPETQVPSPIELDKIQRLVRASPRTYPALALLDDK
                     ALLLNKKGDAFLMYGIEGKSWVCLGDPVGSPREQEELSQKFIDLCRRQKAWPVFYRVG
                     QDHLQPFLDQGLTVLKIGEEALVPLADFQWDKIASSELKAAYQRFQDQEEFDFEALPA
                     GAAGGLLPELKRVSEEWLSKNKARERGFSTGFFDEDYLKRLPLVVVRREGKLAAFAAL
                     WEGHGKAELALDLLRSGADAGAFLEECLLIESMRYGQYQGFGRFSLGAAPLLNMEEGP
                     LSGFKEKPARLFAPYAGGDRLARTRAEMEKFTPEWRPLYLASPGELPLGVALSAVESL
                     "
BASE COUNT          363 a          614 c          642 g          257 t
ORIGIN      
        1 tgcagcgatt ccaccgcgct caaggccacc cccaggggca gctcgccggg cgaggccagg
       61 tatagggggc gccactcggg ggtgaatttt tccatctcgg cgcgggtgcg ggccagccgg
      121 tcgcccccgg cgtagggcgc gaagagccgc gcgggctttt ccttgaagcc cgacagcggg
      181 ccttcctcca tgttcaagag cggcgcggcg cccaggctga agcggccgaa gccctggtac
      241 tggccgtagc gcatgctctc gatcagcagg cattcctcca ggaaggcgcc ggcgtccgcg
      301 ccggaccgca gcaggtccag cgccagctcg gccttgccgt ggccttccca aagcgcggcg
      361 aaggccgcca gtttgccctc gcgccgcacc accaccaggg gcaggcgctt gaggtagtcc
      421 tcgtcgaaaa agcccgtgga aaaaccccgc tcccgggcct tgttcttgct cagccattcc
      481 tccgacaccc gcttcaattc gggcagcagg cctcccgccg cgcccgcggg cagcgcctca
      541 aagtcgaact cttcctggtc ctggaaccgc tggtaggcgg ccttcagttc gctggaggcg
      601 atcttgtccc attggaaatc cgccagcggc accagggcct cctcgccgat cttgagcacg
      661 gtcaggccct ggtccaggaa gggctgcagg tggtcctggc ccacgcggta aaagaccggc
      721 caggccttct gccggcggca caggtcgatg aatttctgcg acagttcctc ctgctcgcgg
      781 ggcgaaccca ccggatcgcc caggcagacc cagctcttgc cctcgatgcc gtacatgagg
      841 aaggcgtcgc ccttcttgtt caagagcagg gccttgtcgt ccaggagcgc cagggccggg
      901 taggtgcgcg gcgaggcccg caccagccgc tggatcttgt ccagctcgat ggggctgggc
      961 acctgggtct cgggctggtc cgggctcagg agccccacca cggcgtaaag caccagggtc
     1021 gccgtggccc ccaacatgga ccgcaggaac cgggcggcgt cctccagcag gtcgaaggtg
     1081 ctccagaggt cgttggtgta gtcctcatag cggtagttga agacgcccac ccagatggac
     1141 cccagccaca cgaaaaggat ggccgtgacc cacagcggcg ggtaacgctg gtagaagagc
     1201 gacgacttcc tggggaaata gcgccgacag ggcagcagca cgccgaagag cgccagcagg
     1261 caaagcgcct ggccgtaatc gaaacccttg aggagggacg ccagcgcgcc cagccccagg
     1321 cagaccaggg tcaggctgta ggcgatgtcc aggcgctgtt gcaggccgcg ggccaagagc
     1381 agcaacatgg cgcccagcac gcccgcggcc aagtgcgagc cttccaagag ccccaggggg
     1441 atgaactcat tgagccacaa gagccgccgg gtcacctcag ggttggcgtt gaagaaaagc
     1501 accagggcgc cggcgaaaaa ggtgaggccg gcgaagccga agggcgccag gtcgggcatc
     1561 cagcggttga agatctgcag ggcccgcttg aagccttcct tgttgtgggt gacctcgtag
     1621 cccgtcaggg ccgaaagccc caagaggaag gggatgaaga aatagaccag gcggaaggcc
     1681 aagaccgccc ccagcacgga cggcgccggc agggccgggg aaaggagcac cacgaaggtg
     1741 gtctccagga tgcccaaccc ccccggcacc tggctcaccg tgccgaagac ctgggccaag
     1801 aggtagatcg agatgaagga cgtgtaggaa aggccggaat gcgggaggaa aaggtagagg
     1861 gtggcgcccg agcagc
//
LOCUS       DASTJJ010000607         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_207356, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000607 DASTJJ010000000
VERSION     DASTJJ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207356"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1678)
                     /locus_tag="VFR02_07395"
     CDS             complement(<1..>1678)
                     /locus_tag="VFR02_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9870301.1"
                     /translation="ALARLFGYERPSELVGKNALDLTDAAGRERFLRPAGPEDTYEIL
                     GRRQDGASFPCQVRDREILYQGRPARVSTFLDLTRLREREQQILESEEKFRRLAEAAT
                     EGVGITLNGKILEVNQVLARMFGYEASEMVGRSALEFTAPESRDLLTRMMLSGTEAPY
                     EATGLRKDGTPITLEISGRMVSFRGHAVRVAVFKDLTERKRLEAGRSKAERARQRSEA
                     RLRAVFNSGPQSMAVLDAQGRLEAFNRNAEEEMERVLGTRLEKDRFLADYLAEPLRGF
                     FLENFRKALAGQTLLTEREVPGRDGRSHWFEFRYHPVLDGDGRATGVCVSTTCVDDRR
                     RAQEALERSEERFRRLFEDSSVGMTLVAADKTFFHVNRAFCQMVGYGAQELRSLTFRD
                     ITDPADLEQNIRLGEGLPEGRGFTMQKRYRHKDGGVVWANITVTPIRDDQGRFLHSLG
                     IIENITEQKRVEAGVRESEERYRRLVEFSPDAVFVYEGGKVLYANPAGLKLLGAREPG
                     ELVGRPLLSLVHPDDRASVEKRVDRIARRRGENPPLEQKFLRLDGQTLEVE"
BASE COUNT          240 a          616 c          545 g          277 t
ORIGIN      
        1 cctccacttc cagggtctgg ccgtccaaac gcaggaactt ctgctccagg ggcgggttct
       61 cgccccggcg ccgggcgatg cggtccacgc gcttttccac cgaggcccgg tcgtccgggt
      121 gcaccaggga caacaacggc cgccccacca attcccccgg ctcccgggcg cccaaaagct
      181 tgagccccgc cgggttggcg tagagcacct tccccccctc gtagacgaag accgcgtccg
      241 gcgagaactc caccagccgg cggtaccgtt cctcgctctc gcgcacgccc gcttccaccc
      301 gtttctgctc ggtgatgttc tcgatgatgc ccaggctgtg caggaaccgc ccctggtcgt
      361 cccggatggg cgtcacggtg atgttggccc agaccacgcc cccgtccttg tgccggtagc
      421 gtttctgcat ggtgaagccg cggccctcgg gcaggccctc gcccaggcgg atgttctgtt
      481 ccaggtcggc gggatcggtg atgtcccgga aggtgaggga cctgagctcc tgcgcgccgt
      541 agcccaccat ctggcagaag gcccggttca cgtggaaaaa ggtcttatcg gcggccacca
      601 gggtcatgcc caccgagctg tcctcgaaga ggcgccggaa gcgttcctcg ctgcgctcca
      661 gggcctcctg ggcgcgccgc cggtcgtcca cgcaggtggt cgagacgcag acccccgtgg
      721 cgcgcccgtc gccgtccagc accgggtggt agcggaactc gaaccagtgg gatcggccgt
      781 cccggcccgg cacctcacgc tcggtcaata gggtctggcc cgccagggcc ttgcggaagt
      841 tctccaggaa aaaaccccgg aggggctcgg ccaggtaatc ggccaggaac cggtccttct
      901 ccaggcgggt gcccagcacc cgttccatct cctcctcggc gttgcggttg aaggcctcca
      961 accgcccctg ggcgtccagg accgccatgg attggggccc gctgttgaag accgcccgca
     1021 gcctcgcctc ggaccgttgt cgggcccgtt ccgccttgga ccgcccggcc tccaggcgtt
     1081 tgcgctcggt caggtccttg aagaccgcca cccgcacggc atggccccgg aaggacacca
     1141 tacggcccga gatctccagc gtgatgggcg tgccgtcctt gcgcaacccc gtggcctcgt
     1201 agggcgcctc ggtgcccgag agcatcatgc gcgtcaagag gtcccggctt tcgggcgcgg
     1261 tgaattccag ggcgctgcgg cccaccatct cggaggcctc gtagccgaac atgcgggcca
     1321 gcacctggtt gacctccagg atcttcccgt tgagggtgat gcccacgccc tcggtggcgg
     1381 cctcggccag gcggcggaat ttttcctcgc tctccaggat ctgttgttcc cgttcccgaa
     1441 ggcgcgtcag gtccaggaac gtggagacgc gcgcgggccg cccctggtag aggatctccc
     1501 ggtcccgcac ctggcagggg aaagaggcgc cgtcctggcg ccgccccagg atctcgtagg
     1561 tgtcctccgg cccggcgggg cgcaggaacc gctcccggcc cgccgcgtcg gtcaggtcca
     1621 atgcgttctt gcccaccaac tcggaaggcc tctcataacc gaacaggcgc gccagggc
//
LOCUS       DASTJJ010000608         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_450917, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000608 DASTJJ010000000
VERSION     DASTJJ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450917"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFR02_07400"
     CDS             complement(<1..977)
                     /locus_tag="VFR02_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9870302.1"
                     /translation="MKIALCQIDTTVGDFDGNTAKVLAALEKAKAQGCGIALFPELTL
                     TGYPPRDLLDRLSFLQAAQRALEKVARGAQGILAVVGTIQENRGSGNPLFNVAVAVKD
                     GRVLYTQKKTLLPNYDVFDEARYFCPAPEPEPPFTYEGLKIGVTICEDIWNVEGLPTH
                     RLYRLDPVQELAKAKPDLVLNLSASPFHAAKLSVRHALLKDLTDRVKAPILYCNLVGG
                     NDELVFDGCSMVMDVGGTLFAEGKAFEEDLLVYDTANPSFQKAPRPLLETETIYQALV
                     TGTRDYVRKCGFEQVLLGLSGGIDSALVACLAAEALGPGNVLGVTMPSP"
     gene            1096..1599
                     /locus_tag="VFR02_07405"
     CDS             1096..1599
                     /locus_tag="VFR02_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HET9870303.1"
                     /translation="MVFGVLADTQAPLTPAQLAALRRAFRGVDALLHAGPVGDLRVLE
                     QLKALAPVRAVSGNAESPQVRSELYIRQAWRQGALTLGLMHGYGKPHGLRAWLLSQFE
                     EGAPQVLVYGRDFEPFARQVGGTYFFNPGSFSGRGPEGSRGPGAPKVGLLFLQGKKVD
                     GQPGIPC"
     gene            1686..2009
                     /gene="erpA"
                     /locus_tag="VFR02_07410"
     CDS             1686..2009
                     /gene="erpA"
                     /locus_tag="VFR02_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET9870304.1"
                     /translation="MVQLTDSAVQKVKDFATKNTAYQGKNFRVYVQGGGCSGFSYGFT
                     FDQKRDDDEINQAGDVEILVDPQSAKYLNGAKIDYVEDLKGAGFVIENPNTTGTCGCG
                     HSVSF"
     gene            2074..2310
                     /locus_tag="VFR02_07415"
     CDS             2074..2310
                     /locus_tag="VFR02_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870305.1"
                     /translation="MEKIELNTDLLADMSGKVTEQSIKSKLPQLDAGAYRGKHVVVKG
                     CAPTWAVMMLQHRLEGVAAGLSFKLFDGKEIGVW"
     gene            2307..>2633
                     /locus_tag="VFR02_07420"
     CDS             2307..>2633
                     /locus_tag="VFR02_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870306.1"
                     /translation="MSGIPWRIIPSGPTPPEGQMEHDRRAFRAFGPGDAPCLRFFQFQ
                     TPTFTLGRLQARRMDLQKLPYPHEARPTGGWTVLHGPQDLCYSILAPTKDARVGGDLL
                     TSYQKIS"
BASE COUNT          446 a          868 c          883 g          436 t
ORIGIN      
        1 tagggtgagg gcatggtcac gcccagcacg ttcccgggcc ccagcgcttc ggcggccagg
       61 caggccacca gggccgagtc gatgccgccc gaaagcccca gcagcacctg ctcgaagccg
      121 cacttgcgca cgtaatcccg cgtgccggtg accagggcct ggtagatggt ctcggtctcc
      181 agcaggggcc ggggcgcctt ttggaacgag gggttggccg tgtcgtagac cagcaggtcc
      241 tcctcgaagg ccttgccctc ggcgaagagc gtgccgccca cgtccatcac catgctgcag
      301 ccgtcaaaga ccagctcgtc gttgccgccc accaggttgc aatagaggat gggcgccttg
      361 acccggtcgg tcaggtcctt caagagcgcg tggcgcacgg acagcttggc ggcgtggaag
      421 ggcgaggccg acaggttcaa gaccaggtcg ggcttggcct tggccaattc ctgcacgggg
      481 tccaggcggt agaggcggtg ggtgggcagg ccctccacgt tccagatgtc ctcgcagatc
      541 gtcacgccga tcttgagccc ctcgtaggtg aagggcggtt cgggctcggg ggcggggcag
      601 aaatagcggg cctcatcgaa gacgtcgtaa ttgggcagga gggtcttctt ttgggtgtag
      661 agcacccggc cgtccttcac ggccaccgcc acgttgaaga gcggattccc cgaaccccgg
      721 ttctcctgga tggtgcccac caccgccagg atgccttggg cgccccgggc caccttctcc
      781 agcgcccgct gggccgcctg caggaacgaa agacggtcca acaggtcccg ggggggatag
      841 ccggtcaggg tcagctcggg gaagagcgcg atgccgcaac cctgggcctt ggccttttcc
      901 agggccgcca gcaccttggc ggtgttgccg tcaaaatccc cgacggtggt atcgatctgg
      961 cagagggcga tcttcatggg gctccggttt cggcctccgc cggttggagg gggcgggcgg
     1021 cggtggtagg ctgggggtcc cagcgaggca gtagtttaag gcggcctccc cgggaagtca
     1081 aaaaggagcc cgaccttggt tttcggcgtc ctggcggaca cccaggcccc cctgaccccc
     1141 gcccaattgg cggcgttgcg ccgcgccttc cggggcgtgg acgccctgct gcacgccggg
     1201 cccgtggggg acctgcgggt gctggaacaa ttgaaggccc tggcgccggt gcgggcggtc
     1261 tcgggcaacg ccgaatcccc ccaggtgcgg tccgagcttt acatccgcca agcctggcgg
     1321 cagggcgccc tgaccctggg gctcatgcac ggctacggca agccccacgg cttgagggcc
     1381 tggcttttgt cccagttcga ggaaggcgcg ccccaggtgc tggtctacgg ccgggatttc
     1441 gagcccttcg cccgccaagt gggcggcacc tatttcttca atcccggctc cttttcgggc
     1501 cgggggcccg agggatcccg gggaccgggc gcgccgaagg tcggattgtt gttcctccag
     1561 ggcaaaaagg tcgatggcca gcccggaatc ccctgttaat cggccccaag gcttgaccgg
     1621 gcgcccggac catcccatat aatgagggta agacatccca ttggggcatt gaaggagacc
     1681 acatcatggt gcaattgacg gattcggcgg tccagaaggt caaggacttc gccaccaaga
     1741 acacggccta ccagggcaag aatttccgcg tgtacgtgca gggcgggggc tgcagcggct
     1801 tttcctacgg cttcaccttc gaccagaagc gcgacgacga cgagatcaac caggccggcg
     1861 acgtggagat cctggtggat ccccagagcg ccaagtacct gaacggcgcc aagatcgact
     1921 acgtggaaga cctgaagggc gcggggttcg tcatcgagaa ccccaacacc accgggacct
     1981 gcggctgcgg gcactcggtc agcttctgat cgaagatcgc ctcaaggcgg gcccttccgg
     2041 gtccgccttt ttttattccc acaaggacgg gccatggaaa agatcgagct gaacaccgac
     2101 ctgctggccg acatgagcgg caaggtgacc gagcagtcca tcaagtccaa gctgccccag
     2161 ctggacgccg gggcctaccg gggcaagcac gtggtggtca agggctgcgc ccccacctgg
     2221 gcggtcatga tgctgcagca ccgtctggag ggcgtggccg ccgggctttc cttcaagctt
     2281 ttcgacggca aggagatcgg cgtctggtga gcggcatccc ctggcggatc atcccctccg
     2341 gccccacgcc ccccgagggc cagatggagc acgaccgccg ggctttccgg gccttcggtc
     2401 cgggggacgc cccctgcctg cggttctttc aattccaaac ccccaccttc accctgggac
     2461 ggctccaagc ccggcggatg gaccttcaaa agctccctta cccccatgag gcgcggccca
     2521 cgggcggttg gaccgtgctc cacggccccc aggacctctg ctattccatc ctcgctccca
     2581 cgaaggacgc ccgggtgggc ggggaccttt tgaccagcta ccaaaagatc agc
//
LOCUS       DASTJJ010000609         1004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_941040, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000609 DASTJJ010000000
VERSION     DASTJJ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941040"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFR02_07425"
     CDS             complement(<1..231)
                     /locus_tag="VFR02_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HET9870307.1"
                     /translation="MSLYDGQDKTPLYDAIVDYANENKISFHTPGHKHGKGILKPFRQ
                     LVGEKVFQLDLSVMSEIDSLHEPSTVIKEAQVL"
     gene            complement(232..>1004)
                     /locus_tag="VFR02_07430"
     CDS             complement(232..>1004)
                     /locus_tag="VFR02_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9870308.1"
                     /translation="AQGDEFIYHENLVHPAMVSCPNPKNVVIIGGGEGATLYEVLRHK
                     TVEKAWMIDIDERVVELCEKYMPEWHRGAFKDKRTKLLFEDGRKFLEKSKVKFDVIII
                     DIPEPVEEGPAYLLYTEEFYKMVAGKLSENGMVALQSGCSAEKELRCLAAIQTTLRRV
                     FPMVYSWPANVPSFDIPWSFTMASNQVDPKKLKRAEVDRILKERKVGRLRYYDGETHE
                     GYFFLPKYMRDAFRADHTVIRDKQPLTYQFMGEEKNAL"
BASE COUNT          171 a          293 c          310 g          230 t
ORIGIN      
        1 cagcacctgg gcttccttga tgaccgtgct gggctcgtgg agcgagtcga tctcgctcat
       61 caccgacagg tcgagttgga acaccttctc gcccaccagc tggcggaaag gcttcaggat
      121 gcctttgccg tgcttgtggc ccggggtgtg gaaggagatc ttgttttcgt tggcgtagtc
      181 gacgatggcg tcatagaggg gggtcttgtc ctgcccgtca taaagtgaca tttagagcgc
      241 gttcttctcc tctcccatga attgataggt gaggggctgc ttgtcgcgga tgaccgtatg
      301 gtccgcccgg aaggcgtccc gcatgtactt gggcaggaaa aagtaaccct cgtgggtctc
      361 gccgtcgtaa tagcgcaggc gcccgacctt gcgttccttc aggatgcggt ccacctcggc
      421 gcgtttgagc ttcttgggat cgacctggtt ggaggccatg gtgaagctcc aggggatgtc
      481 gaaggaaggc acgttggccg gccaagagta gaccatggga aagacccgcc gcagggtggt
      541 ctggatggcc gccaggcagc gcagttcctt ctcggccgaa cagcccgact gcagggccac
      601 catgccgttc tccgaaagct tgcccgcgac catcttgtag aactcttcgg tataaagcag
      661 gtaggccggc ccctcttcca cgggttcggg gatgtcgatg atgatcacgt cgaacttcac
      721 cttgctcttt tcgaggaact tgcgcccgtc ctcgaacaaa agcttggtgc gcttgtcctt
      781 gaaggcgccc cggtgccatt cgggcatgta tttttcgcac aattccacca cgcgctcgtc
      841 gatgtcgatc atccaggcct tttccaccgt cttgtgccgc agcacctcgt agagggtggc
      901 accttccccg cccccgatga tgaccacgtt cttcgggttg gggcagctga ccatggccgg
      961 atggaccagg ttctcgtggt agatgaactc gtcgccctgg gccg
//
LOCUS       DASTJJ010000610         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1307224, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000610 DASTJJ010000000
VERSION     DASTJJ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307224"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..655
                     /locus_tag="VFR02_07435"
     CDS             155..655
                     /locus_tag="VFR02_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9870309.1"
                     /translation="MIEMKVRGIAVDPNLKTPAVILTDEEEKRYLPIWIGVAEATAIF
                     IQLNEQTLPRPMTHDLLKNVIETLGGKVNKVLVNDINQNTFFARVFLETEAGEKFEVD
                     ARPSDAIALALRCDSPIFVAEKVVINATIVDKEKYKEEMSEFKKFLENLTPTDFKDLP
                     GPNSNT"
     gene            659..976
                     /locus_tag="VFR02_07440"
     CDS             659..976
                     /locus_tag="VFR02_07440"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HET9870310.1"
                     /translation="MAAAESRYQLTDKHFILLFHTTHETMHAEALLKEVRMRFKLIPR
                     PRDIQDDCGLGLVIVKEDRPKVEKLLPPKGAYIKAAFHIEKDGSWTRENYPPAPISYD
                     SPS"
     gene            922..1164
                     /locus_tag="VFR02_07445"
     CDS             922..1164
                     /locus_tag="VFR02_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870311.1"
                     /translation="MDQGKLPPRAHLLRLSLLGLNFALSIFTGLALGWAAQKFLHWGD
                     GAVLAGMVLGIVAGYWGLFAELKKLRIPKSPPRDPR"
     gene            1151..1528
                     /locus_tag="VFR02_07450"
     CDS             1151..1528
                     /locus_tag="VFR02_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870312.1"
                     /translation="MTPAKMDWTTPVWRLSLALGALGALAAWAWGRADLALGWGLGSA
                     GSVAHFAALHRLTKRLTAGAGRGPGSFWFLHLARWALFLLAAWLFWRVSPACFLGAAL
                     AYLTCLALLLAAAFRAPKPGKSS"
     gene            1692..2507
                     /gene="atpB"
                     /locus_tag="VFR02_07455"
     CDS             1692..2507
                     /gene="atpB"
                     /locus_tag="VFR02_07455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9870313.1"
                     /translation="MDKALTPIVIPFHFLDFHNLINCWTLSLVSFFFLFLLLAWARFK
                     PQWIPHGVQNFVELSIEFVWNMAEGQVGRHTAFFFPLFFTLFFFVFFSNLLGLVPGLM
                     SPTSRIDVNLGMVLIVMVSVHVLDIRVNGLRGYLAHYLPPKIQADPKAHLFLRVVLRL
                     VSVFLLLMMPFIHLIGELAKPLSLTMRLFGNMLAKEILLAVLLLLSLDFWAGPGVMKA
                     VASFPFLLRVCIVVYGIFVCFIQAFVFMMLSMVYIGTNVKDHGEEGDGHPAAA"
BASE COUNT          429 a          858 c          771 g          534 t
ORIGIN      
        1 cccacggccc ggggctgaat cgggcctttt tggacggggt cgttgaaagg gcccgggacc
       61 gaccctataa ttgcggggac ggcccaccct tgtttccggc gtccgccggc ggctgaattg
      121 gaacggagcc ccaaaccatg aacgagacca aggcatgatc gaaatgaaag tccgcggcat
      181 cgcggtcgac cccaacctca agacccccgc ggtcatcctg accgacgagg aagagaagcg
      241 ctacctgccc atctggatcg gcgtggccga ggccaccgcc atcttcatcc agctcaatga
      301 gcagaccctt ccccggccca tgacccacga cctgctgaag aacgtcatcg agaccctggg
      361 cggcaaggtc aacaaggtgc tggtcaacga catcaaccag aacaccttct tcgcccgggt
      421 cttcctggag accgaggcgg gggagaagtt cgaggtcgac gcccgtccct cggacgccat
      481 cgcgcttgcc ctgcgttgcg attcgcccat tttcgtggcc gagaaggtgg tcatcaacgc
      541 caccatcgtg gacaaggaaa agtacaagga agagatgagc gagttcaaga agttcctgga
      601 gaacctcact cccacggact tcaaggacct gcccgggccc aactcgaaca cctaggccat
      661 ggccgccgcc gagtcccgct accagctgac cgacaagcac ttcatcctgc tcttccacac
      721 cacccacgag accatgcacg ccgaggccct gctcaaggag gtccggatgc gcttcaagct
      781 catcccccgg ccccgggaca tccaggacga ctgcggcctg gggctggtca tcgtgaagga
      841 agaccggccc aaggtggaga agctgctgcc cccgaagggg gcctacatca aggcggcgtt
      901 ccatatcgaa aaggacggct catggaccag ggaaaactac ccccccgcgc ccatctccta
      961 cgactctccc tcctagggct caacttcgcc ctgtccatct tcacggggtt ggccctgggc
     1021 tgggccgccc aaaagttcct gcattggggc gacggcgcgg tgctcgccgg catggtcttg
     1081 gggatcgtgg cggggtactg gggcctcttc gctgaattga agaaactgcg catccccaaa
     1141 tccccgcccc gtgacccccg ctaagatgga ttggaccacg ccggtctggc gcttgagcct
     1201 ggccctgggg gccctgggcg cgctcgcggc ctgggcctgg ggccgggcgg acctggcgtt
     1261 gggctggggc ctgggaagcg ccgggtcggt ggcgcatttc gccgcgctcc accgcctcac
     1321 gaagcggttg acggcggggg cggggcgggg cccgggatcc ttttggttcc tccacctggc
     1381 gcgttgggcg ctttttcttt tggccgcctg gctcttttgg agggtctcgc ccgcctgttt
     1441 cctgggggcg gccctcgcct acctgacctg tctggcgctg ctcttggccg ccgcgttccg
     1501 cgcccccaag cccggcaaaa gttcctgaaa aaccaccatt tcccgcgggc gcttttgtcc
     1561 gggcagggtt gatcctcacc aagggaccca ttagactacc cccgctcctg gacatctttg
     1621 accgattttt tcgttaaaat gccccagccc ccaagcccgg gggtgttcga ttttttagga
     1681 aaggttccga cgtggacaag gccctcaccc ccatcgtgat ccccttccac ttcctggact
     1741 tccacaacct catcaactgc tggaccctct cgctggtgag cttcttcttc ctgttccttt
     1801 tattggcctg ggcccgcttc aagccccaat ggatccccca tggcgtgcag aacttcgtgg
     1861 agctttccat cgaattcgtc tggaacatgg ccgagggcca agtgggccgc cacacggcct
     1921 tctttttccc cctctttttc accctgttct tcttcgtgtt cttctcgaac ctgctgggcc
     1981 tggtgccggg cctcatgtcg cccaccagcc gcatcgacgt gaacctgggg atggtgttga
     2041 tcgtgatggt ctcggtgcac gtgctggaca tccgggtcaa cggcctgagg ggctacttgg
     2101 cccattacct gccccccaag atccaggccg accccaaggc gcacctgttc ctgagggtgg
     2161 tgctgcggtt ggtgtcggtg ttcctcctct tgatgatgcc cttcatccac ctgatcggcg
     2221 agctggccaa gcccctgtcc ttgaccatgc ggcttttcgg gaacatgctg gccaaggaga
     2281 tcctgttggc cgtactgctg ctcttgtccc tcgatttctg ggccgggccg ggcgtcatga
     2341 aggcggtggc ctccttcccc ttcctgctgc gggtgtgcat cgtggtgtac ggcatcttcg
     2401 tgtgcttcat ccaggctttc gtgttcatga tgttgtccat ggtctatatc ggcaccaacg
     2461 tgaaggacca tggggaggag ggcgacggcc atccggccgc ggcctaggcg gttcgggttt
     2521 cgacgggccc cctcgggggg cgcgggtcca acaatctcat aaggggtgca gcatgaaaaa
     2581 gtctttgttt ct
//
LOCUS       DASTJJ010000611         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_816867, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000611 DASTJJ010000000
VERSION     DASTJJ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816867"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFR02_07460"
     CDS             complement(<1..1001)
                     /locus_tag="VFR02_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9870314.1"
                     /translation="MTRPRRLLLWAGLTGLLFLGMALSLHAGEGRLLAGFVAGFLAAV
                     LLYFLYPLSSDPEPGPEGPPPTAAETVAAPPNPTLDGMLQRPGSNESMLNVILNSMGE
                     GVLLLNQADRVVLMNPSAEKVLGLQARDARGRHYLEVLRHPVLSDLLARAKSGEGLAT
                     GELEFSARDERTFAVSAAAIRVEGGALLGQIMVFSDITSLKKLMRMRTDFAANVSHEL
                     KTPLTAILGYVETLLAGAIDDKKNRVQFLGKISAQAQRLHALIADVLQLSAIESGSYL
                     EASEAVDLSEVAQQAVELLKAKWEARGIRVVQEVPAGRKVWGQKEGLFHVVENLLDNA
                     "
     gene            complement(1045..1728)
                     /locus_tag="VFR02_07465"
     CDS             complement(1045..1728)
                     /locus_tag="VFR02_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870315.1"
                     /translation="MKQIFVVEDEKDLSDLLTYNLEKDGYRVLSEMDGESAMRKIPDK
                     MPDLVLLDLMLPKADGLTVCKTLKGNPKTAHIPVVMLTAKGEESDKIVGLELGADDYI
                     TKPFSVKELLARIRAVLRRFDKAQEGEPLQKFKDLVLNRQKHEVTLKTQGLDLTAKEF
                     ELLDYFLTHAGRVLSRDVLLNNVWGYDYFGTTRTVDVHVRRLREKLGTYQKYIQTVKG
                     YGYLFKDEK"
     gene            1998..2396
                     /locus_tag="VFR02_07470"
     CDS             1998..2396
                     /locus_tag="VFR02_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870316.1"
                     /translation="MFKVMVVDEDVRQLREIADEIGVLYNLLICGRGAKAAGLFQAFQ
                     PDALILDPSAEGLDTQGLIAQARNRPGLARTPVLLLAQFTTIRHIEKSLDWGADYVFS
                     KPCPGDRIGRKLAECLEKADRYRQSQLVEL"
     gene            2491..>2778
                     /locus_tag="VFR02_07475"
     CDS             2491..>2778
                     /locus_tag="VFR02_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9870317.1"
                     /translation="MGKADLHLHTDFSDGLHSPREMVQAAARAGLDHIAVTDHDTMEG
                     AFRAQEYAKRRSDLGVQVILGEEVSTLNGHVIGLFLKGFIPPRLSAKRTVEL"
BASE COUNT          479 a          958 c          839 g          502 t
ORIGIN      
        1 gcgttgtcca gcaggttctc caccacgtgg aagagccctt ccttctggcc ccagaccttg
       61 cgccccgccg gcacctcctg gaccacgcgg atgccccgcg cttcccactt ggccttgagc
      121 agctccaccg cctgttgggc cacttccgac aggtccaccg cctccgaggc ctccaggtag
      181 gacccgctct cgatggcgga caattgcagc acgtccgcga tcaaggcgtg caggcgttgg
      241 gcctgggccg agatcttccc caggaattgc acccggttct tcttgtcgtc gatggcgccc
      301 gccagcaacg tctccacgta gcccaggatg gccgtgaggg gggtcttcag ttcatgggag
      361 acgttggcgg cgaaatcggt gcgcatgcgc atgagcttct tgagcgaggt gatgtccgaa
      421 aagaccatga tctggcccag cagcgccccc ccttccaccc ggatggcggc cgcgctgacg
      481 gcgaaggtcc tctcgtcccg ggcggagaac tccagctccc ccgtggccag cccttcgccc
      541 gacttggcgc gcgccaacag gtccgacagc acgggatgcc gaagcacttc caggtaatgg
      601 cggccccggg cgtcccgcgc ctgcagcccc aggaccttct cggcggaagg gttcatgagc
      661 accacccggt ccgcctggtt caggagcagc accccctcgc ccatgctgtt gaggatgacg
      721 ttgagcatgg attcgttcga acccggccgc tggagcatcc cgtccagggt ggggttgggg
      781 ggcgccgcca ccgtctccgc ggccgtgggc gggggcccct ccggacccgg ctcgggatcc
      841 gaggagaggg gatacaggaa gtacagcagg acggcggcca ggaatcccgc cacgaacccg
      901 gccaagagcc ggccttcccc ggcgtgcagg gacagggcca taccgaggaa caacaacccg
      961 gtcaaaccgg cccaaagcag caaccgccgc ggcctcgtca tggaccgtcc tcgcttggat
     1021 gggatgggtg gggaaagacc gccgttactt ctcgtccttg aacaggtagc cgtagccctt
     1081 gacggtctgg atgtactttt ggtaggtccc cagcttttcc cggagccggc gcacgtgcac
     1141 gtccaccgtc cgggtggtgc cgaagtaatc atacccccaa acgttgttca ggagaacatc
     1201 ccgggaaagg acccggcccg cgtgggtcag gaaatagtcc aaaagctcga attccttcgc
     1261 ggtcaggtcc aacccctggg tcttcagggt gacctcatgc ttctgccggt tcaacaccag
     1321 gtccttgaac ttctgcaggg gttcgccttc ctgggccttg tcgaagcgcc ggaggaccgc
     1381 ccggatgcgg gccaagagtt ccttcaccga gaagggcttg gtgatgtagt cgtccgcccc
     1441 cagttccagc cccacgatct tgtccgattc ctcgcccttg gcggtcagca tgaccaccgg
     1501 gatgtgggcg gtcttggggt tccccttgag ggtcttgcag accgtcaatc cgtcggcctt
     1561 gggcagcatg aggtcgagca gcaccaggtc gggcatcttg tccgggatct tgcgcatcgc
     1621 cgactcgccg tccatctcgc tcaagacccg gtagccgtcc ttttccaggt tataggtcag
     1681 caggtcggac aggtccttct cgtcctccac cacgaatatc tgtttcatgc gcacaccttg
     1741 gttcctttcc cggaatgccg ggaaaccgtt ctttccctga atggcgggga ttatagccaa
     1801 gcccccccgg gtccatgtta aaagcttgta aaacccccca agccccgccg ccggagggcg
     1861 gtttcccctg gttttttcag ggcctgcggc cgccgcccgc cgccatgaat taacccttct
     1921 ttaacccccg ctttaaggcc cggacccggg gcccgcctag ggtgcggcca ttccataacg
     1981 aaagatcggg aggacccatg ttcaaggtca tggtcgtgga cgaggatgtc cgtcaactgc
     2041 gcgagatcgc cgacgagatc ggcgtgcttt acaacctgct catctgcggc cggggcgcca
     2101 aggccgccgg ccttttccag gccttccaac ccgacgccct gatcctggac ccctccgccg
     2161 aggggctgga cacccagggc ctgatcgccc aggcccgcaa ccgccccggg ttggcccgca
     2221 cccccgtgct gctgctggcc caattcacca ccatccgcca catcgagaag agcctggatt
     2281 ggggcgcgga ttacgtcttt tccaaaccct gtccggggga ccgcatcggc cgcaaactcg
     2341 ccgagtgcct ggaaaaggcc gaccgctacc ggcaaagcca gctggtggag ctttaagctt
     2401 ccacccccat ccctgggggc atttgttacc acaacgtaac aacgtgtttc gcggcgcctt
     2461 acaaggggat cctatgctgg cgcccgtacc atgggcaaag cggacttaca cctccacacc
     2521 gacttttccg acgggcttca cagccctcgg gaaatggtgc aagcggccgc ccgggcgggg
     2581 ctggatcata tcgccgtcac cgaccacgac accatggagg gggccttccg ggcccaggag
     2641 tacgccaaac gccggtccga cctgggcgtc caggtcatcc tgggcgagga ggtcagcacc
     2701 ctcaacggcc acgtcatcgg cctgttcttg aagggcttca tcccgccgcg cctgagcgcc
     2761 aaacgcacgg tggagctc
//
LOCUS       DASTJJ010000612         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_941630, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000612 DASTJJ010000000
VERSION     DASTJJ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941630"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFR02_07480"
     CDS             <1..546
                     /locus_tag="VFR02_07480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9870318.1"
                     /translation="VTGVLGPVQFQVLTRAQWESFPWRGFSLSALAVQVDGFAALEEK
                     YGHAFGAGLLQDVAWLLHFTTREGDLVGREGPAFLCLLPRCDADEAITVVRRLQDSLG
                     RQTFRAGDQVVHVSVGAGIATLDSPRGADLETLFHTARRALAGALAEGPHRFHLAPVP
                     VEIEAFPTQPLPSPGDGAERA"
     gene            718..951
                     /locus_tag="VFR02_07485"
     CDS             718..951
                     /locus_tag="VFR02_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9870319.1"
                     /translation="MLNFNQKDPRDVELETTVCRAVEGMAAGLHVNAQAGHVSLSGMV
                     DDYSTKRDISAIVRDLAGVKTLSNGIRVTPIGD"
     gene            complement(988..>1304)
                     /locus_tag="VFR02_07490"
     CDS             complement(988..>1304)
                     /locus_tag="VFR02_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870320.1"
                     /translation="WTPQGVRESGLATNPVHVLDLALVLPGMAWTGTALLRGKGWAYR
                     FFPAWMVFIILMAWALAGMAAALWARGLAAGLAPALIFLAVSAVGSAVLAVYWRKFTA
                     LA"
BASE COUNT          236 a          437 c          421 g          210 t
ORIGIN      
        1 gtcacgggcg tgctggggcc cgtgcagttc caggtgctca cccgggccca gtgggagtcc
       61 ttcccctgga ggggtttttc cctctcggcc ctggccgtcc aggtggacgg tttcgccgcg
      121 ttggaggaaa aatacggcca cgccttcggg gccgggttgc tgcaggacgt ggcctggctg
      181 ttgcacttca ccacccggga gggggacctg gtgggccggg aaggcccggc cttcctctgc
      241 ctgcttcccc gctgcgacgc cgacgaggcc atcacggtgg tgaggcgcct gcaggacagc
      301 ctgggccgcc agaccttccg cgccggggac caggtcgtcc atgtgagcgt gggcgccggg
      361 atcgccacct tggattcccc ccggggcgcg gacctggaaa ccctgttcca caccgcgcgc
      421 cgggccctgg cgggggcact ggcggaaggt ccgcaccggt tccacctggc gccggtcccc
      481 gtcgagatcg aagccttccc gacccagccc ctcccctcac cgggggacgg ggccgagcgg
      541 gcctagaagt cattttaaaa actcttttac cgcagagacg cagaggcgcc aagtgaagac
      601 aaggggcgaa aaaccgagga atcgacatta tttttcctgc gtctccgcgt ctctgcggtt
      661 aaaaattttc gcttttaggg ttttgaaaca ggttctagat cattccagga ggatgctatg
      721 ctgaacttca accagaagga tccgagggac gtggagctgg aaacgacggt ctgccgggcg
      781 gtcgagggga tggccgccgg cctgcacgtg aacgcgcagg ccggccacgt gtccctctcg
      841 ggcatggtgg atgattattc caccaaacgc gacatctccg ccatcgtgcg ggacttggcc
      901 ggggtcaaga ccctgtccaa cggcatccgg gtgacgccca tcggcgattg acccgaacgg
      961 cggtcggagg ccgggcttgg ctgcctttca ggcaagggcc gtgaatttcc tccaataaac
     1021 cgccagcacc gccgatccca cggccgacac ggccaggaag atcagggccg gcgccaagcc
     1081 agccgccaag ccgcgggccc aaagcgccgc cgccatcccc gccagggccc aagccatcag
     1141 gatgatgaag accatccaag ccgggaaaaa gcggtaagcc cagcccttgc cccggagcaa
     1201 cgccgttccc gtccaggcca tgcccggcaa taccaaagcc aggtccaata cgtgcacggg
     1261 attggtggcc aaccccgatt cacggacacc ctgcggcgtc catc
//
LOCUS       DASTJJ010000613         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_817172, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000613 DASTJJ010000000
VERSION     DASTJJ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817172"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFR02_07495"
     CDS             <1..217
                     /locus_tag="VFR02_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9870321.1"
                     /translation="QGQATDIQIQAEEIMKTKKQLNGILSLHTGQSLDKIEKDVERDH
                     FLSADEAKAYGLVDEVIVKRPESKKDK"
     gene            227..1492
                     /gene="clpX"
                     /locus_tag="VFR02_07500"
     CDS             227..1492
                     /gene="clpX"
                     /locus_tag="VFR02_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9870322.1"
                     /translation="MAGDKERTNYLRCSFCGKTQEEVKKLVAGPTVYICNECIALCND
                     ILKEEEKDDAKGGAPKAKMVIPKPAEINAILDQYVIGQEKAKRILSVAVHNHYKRLTF
                     KSGPQDVELQKSNVLLIGPTGVGKTLLAQTLARILDVPFAVADATTLTEAGYVGEDVE
                     NIILKLLQNAEFDVQRAERGIIYIDEIDKISRKSENPSITRDVSGEGVQQALLKIIEG
                     TEVNVPPQGGRKHPHQEYIKVDSSNILFICGGAFVGLEKVVEARLGRRSIGFKGESVS
                     LVDKKLGELFAKVQPEDLIKYGLIPEFIGRLPVFAPLHELDEASLVKVLTEPKNALVK
                     QYQKYFEMEGAKLKFTPEALKAVAREAIRRQSGARGLRAILEEAMMDLMYELPSRKDI
                     KEVVATEGFILKKEEPILVYQKGRGENTA"
BASE COUNT          368 a          505 c          530 g          249 t
ORIGIN      
        1 ccagggccag gccacggaca tccagatcca ggccgaggag atcatgaaga ccaagaaaca
       61 gctcaacggc atcctttccc tgcacacggg ccaatccctt gataaaatcg agaaggacgt
      121 ggaacgggac cacttcctgt cggccgacga ggccaaggcc tacggcctgg tggacgaggt
      181 gatcgtgaaa cggcccgaat cgaaaaaaga caagtaaagg gcggtcatgg cgggcgacaa
      241 ggaacggaca aattacctgc gctgctcctt ctgcggcaag acccaggagg aggtcaagaa
      301 gctggtggcg ggccccacgg tctacatctg caacgagtgc atcgcgcttt gcaacgacat
      361 cctgaaggaa gaggaaaagg atgacgccaa gggcggcgcg cccaaggcca agatggtcat
      421 ccccaagccc gcggagatca acgccatcct ggaccagtac gtcatcggcc aggaaaaggc
      481 caagcgcatc ctgtcggtgg ccgtgcacaa ccactacaag cgcctgacct tcaagtcggg
      541 cccccaggac gtggagctgc aaaagagcaa cgtgctcctg atcgggccca cgggcgtggg
      601 caagaccctc ctggcccaga ccctggcccg catcctggac gtgcccttcg cggtggcgga
      661 cgccaccacc ttgaccgagg ccggctacgt gggcgaggac gtggagaaca tcatcctcaa
      721 gctgctgcag aacgccgagt tcgacgtgca gcgcgccgag cgggggatca tctacatcga
      781 cgagatcgac aagatcagcc gcaagtccga gaaccccagc atcacccggg acgtgtcggg
      841 cgagggcgtg cagcaggcgc tcctgaagat catcgagggc accgaggtca acgtgccgcc
      901 ccagggcggg cgcaagcacc cccaccagga atacatcaag gtcgattcga gcaacatcct
      961 cttcatctgc ggcggcgcct tcgtggggct ggagaaggtg gtggaggccc ggttggggcg
     1021 gcgcagcatc ggcttcaagg gcgagtcggt cagcctggtg gacaagaagc tgggcgagct
     1081 cttcgccaag gtgcagcccg aggacctgat caagtacggc ctcatccccg agttcatcgg
     1141 ccggctcccg gtcttcgcgc ccctgcatga gctggacgag gcctccttgg tcaaggtgct
     1201 cactgaaccc aagaacgccc tggtgaagca ataccagaag tatttcgaga tggagggcgc
     1261 caagctcaag ttcaccccgg aggccctgaa ggccgtggcc cgcgaggcca tccggcgcca
     1321 gtcgggcgcc cggggcctgc gcgccatcct ggaagaggcc atgatggacc tgatgtacga
     1381 gctgccttcc cgcaaggaca tcaaggaagt ggtggccacc gagggcttta tcctgaagaa
     1441 ggaagagccc atcctggtct accagaaggg ccgcggcgag aatacggcct aggcggggca
     1501 ttttcaccgc aaagacgcaa agacgcggtg aaggcgaagg ccgaccttcg aatttccatt
     1561 ttggccggac cctgccgtgg gttgggttcc gatctttccg tttggctgcg cctccgcgtc
     1621 cctgcggtgg aaaaagcctt gaaggttctt ga
//
LOCUS       DASTJJ010000614         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1063281, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000614 DASTJJ010000000
VERSION     DASTJJ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063281"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1099
                     /gene="dapA"
                     /locus_tag="VFR02_07505"
     CDS             206..1099
                     /gene="dapA"
                     /locus_tag="VFR02_07505"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9870323.1"
                     /translation="MFRGAFTALVTPFKNGRVDYAALESLIESQITRGIDGLVPCGTT
                     GESPTLSEPEHDEVIHFTVKAVKKRVPVIAGTGSNSTEEAIRYTRHALDVGADAALLA
                     CPYYNKPSQRGLVAHFNAVAAAAPMPLVLYNIPGRTGINMAPETIAELSKNPRFVGVK
                     EASGNLEQMIKIRALCAPEFDLISGDDTLTLPILAVGGTAVISVTSHLLPGETSRIVH
                     AYLEGKTGESKDLFFKTFGLVKAVMGADVNPVGVKTALALRGEIAEEFRLPLVPASPE
                     AKDSIRQALDQAGLLATAGRR"
     gene            1099..1920
                     /gene="dapB"
                     /locus_tag="VFR02_07510"
     CDS             1099..1920
                     /gene="dapB"
                     /locus_tag="VFR02_07510"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9870324.1"
                     /translation="MALRLAVSGATGRMGQTILGLAAKDPRFGIVGALESPQHAGLGK
                     PLDLILPSLGPQKATLEADPAKFAVKPQVLIDFTHPTATTLYLDQAAALGFGMVIGTT
                     GLTEADKEKIKQASARVPVVQATNMSLGMNLLFALVEEAAAKLGPAYDAEIVEAHHHF
                     KKDAPSGSALSLGESVARAWGVKLSEFSTHGREGQVGERKAGTIGFHAVRGGDIVGDH
                     TVMFAGTGERLELTHRAQSRESFAQGALTAALFLGGKAKGLFDMLDVLGLRRTSK"
     gene            2029..2790
                     /locus_tag="VFR02_07515"
     CDS             2029..2790
                     /locus_tag="VFR02_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9870325.1"
                     /translation="MRIARLLHNQKPRWAMLLESVDTYRCFTSDEPWANPVLDATSYP
                     LKPEKLLAPALPTKIVAVGLNYRDHIQEMKHDDHAEPVLFLKPPSSLLNPGGTILYPP
                     SSNRVDYEAELAVVLKTRLKDATPKQALEAVWGFTCCNDVTARDLQKVDGQWARAKGF
                     DSFCPLGPWLVTDFTEGSQRVTARLNGEVKQDAKVSQRIWKTAELLSFASKVMTLEPL
                     DVLTTGTPSGIGPMKAGDAVEVEIEGIGVLRNTVG"
     gene            2949..>3615
                     /locus_tag="VFR02_07520"
     CDS             2949..>3615
                     /locus_tag="VFR02_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9870326.1"
                     /translation="MDFAPADRLKKLPPYLFIEIDKKKKAAIEKGVDIISLGIGDPDL
                     ATPDFIIEALYKAAKDKANHQYPLGTGLPKFREAVAKWYQRRFNVTLEPKTEVVALIG
                     SKEGIGHLPVGVLNPGDVCLVPSPGYPVYHAGTLFAGGESYFMPLLEKNGFLPDLDAI
                     PAEALKRAKLIWTNSPNNPTSALAGKDFYAKVIEFAKKHRLIVANDAAYTEIYYDGKK
                     PVSF"
BASE COUNT          690 a         1261 c         1090 g          574 t
ORIGIN      
        1 cgaggtggtg gtgcggggca accgcttcgc ggtggcccgc cggcgccaga cggtggagga
       61 cctggtgcgg ctggaaacga tccggcgctg agcggcccaa acaccacttg cgatgggtgg
      121 ggaaatttct taatataact ccaccatcgc ggcccccttc gcagggggtc ttgtcgtttc
      181 aggcggtgga agaggagaaa ataaaatgtt tcgaggcgcc ttcacggccc ttgtcacgcc
      241 attcaagaac ggccgggtgg attacgccgc cctggaatcc ctcattgaat cccagatcac
      301 ccgcggcatc gacggcctgg tgccctgcgg caccacgggc gaatcgccca cgctttccga
      361 accggaacac gacgaggtca tccatttcac ggtaaaggcc gtcaagaagc gcgtgcccgt
      421 catcgccggc acgggctcca attccaccga ggaagccatc cgctacaccc gccacgccct
      481 ggacgtgggc gcggacgccg cactgctggc ctgcccctac tacaacaagc ccagccaacg
      541 gggcctggtc gcccatttca acgccgtggc cgccgccgcg cccatgccgc tggtgctcta
      601 caacatcccg ggccgcacgg gcatcaacat ggcgcccgag accatcgccg aactgtccaa
      661 gaacccccgc ttcgtgggcg tgaaggaagc cagcggcaac ctggagcaga tgatcaagat
      721 ccgcgcgctc tgcgcgcccg aattcgacct gatctcgggg gacgacaccc tgaccctgcc
      781 catcctggcc gtgggcggca cggccgtcat ctcggtgacc tcccacctgc tgcccggcga
      841 gacctcccgc atcgtccacg cctacctgga aggcaagacc ggcgagtcga aggacctttt
      901 cttcaagacc ttcggcctgg tcaaggccgt catgggcgcc gacgtgaacc ccgtgggcgt
      961 gaagaccgcc ctggccctgc gcggcgagat cgccgaggaa ttccgcctgc ccctggtgcc
     1021 cgcctcgccc gaggccaagg actccatccg ccaggcgctg gaccaggccg gcctgctcgc
     1081 caccgccggg cgccgctgat ggcgctccga ctcgcggtct ccggcgccac cggccggatg
     1141 ggccagacca tcctgggact ggccgccaag gacccccgct tcgggatcgt gggcgccctg
     1201 gaaagccccc agcacgccgg cttgggcaag ccgctggacc tgatcctgcc ttccctgggc
     1261 ccccaaaagg ccacgctgga ggccgacccc gcgaaattcg ccgtgaagcc ccaggtgctc
     1321 atcgacttca cccatcccac ggccaccacg ctctacctgg accaggccgc ggcgctgggc
     1381 ttcggcatgg tcatcggcac cacgggcctg acggaggccg acaaggaaaa gatcaagcag
     1441 gcctccgccc gggtgcccgt cgtgcaggcc accaacatga gcctgggcat gaacctgctc
     1501 ttcgccctgg tggaagaggc cgccgccaaa ctgggccccg cctatgacgc cgagatcgtg
     1561 gaggcccacc accatttcaa gaaggacgcc ccctcggggt cggccctttc cttgggcgaa
     1621 tcggtggccc gcgcctgggg cgtgaagctt tcggagtttt ccacccacgg ccgggaaggc
     1681 caggtgggcg agcgcaaggc cggcaccatc ggtttccacg ccgtgcgcgg cggggacatc
     1741 gtgggggacc acaccgtgat gttcgccggc accggcgagc gcctggaact gacccaccgc
     1801 gcccagagcc gggaatcctt cgcgcagggc gcgctgacgg ccgccctttt cctcggcggc
     1861 aaggccaagg gcctcttcga catgctggac gtcctagggt tgcgccgaac aagcaaataa
     1921 attttcaccg ccaagacgcc aagactccaa gaaaggcgaa tgattttggg acataaaaat
     1981 tatttcttgg tgtcttggtg tcttggtggt tgatccaggt cttttgcctt gaggatcgcc
     2041 cggctcctcc acaaccaaaa accgcgctgg gccatgctgc tggaatcggt ggacacctac
     2101 cgctgcttca cctcggacga accctgggcc aacccggtcc tggacgccac ctcctacccg
     2161 ctcaagcccg agaaactcct ggcgccggcc ctgcccacca agatcgtggc ggtgggcctg
     2221 aactaccggg accacatcca ggaaatgaag cacgacgacc acgccgaacc cgtgctcttc
     2281 ctcaaaccgc cctcgagctt gttgaatccc ggcgggacca tcctgtatcc tccctcctcg
     2341 aacagggtcg actatgaggc cgagctggcc gtggtgctga agacgcgcct caaagacgcc
     2401 acgccgaagc aggccctgga ggccgtctgg ggattcacct gctgcaacga cgtgaccgcc
     2461 cgcgacctcc agaaggtgga cggccaatgg gcccgggcca agggcttcga ctccttctgc
     2521 cccttgggcc cttggttggt gaccgacttc accgaggggt cccagcgcgt gacggcccgg
     2581 ctgaacgggg aagtgaagca ggacgccaag gtctcccagc gcatttggaa gaccgccgag
     2641 ctcctgtcct tcgcctccaa ggtcatgacc ctggagcccc tggacgtgct gaccaccggc
     2701 accccctcgg gcatcggccc catgaaggcc ggggacgcgg tggaggtgga gatcgagggc
     2761 atcggagtat tgcgcaacac cgtcggatga gaccaaaacc cgttctcacc acgaagttca
     2821 cgaagggcac gaagtagggg cgcggtgaaa atcttcctgg atacagcgtc gaggtcgggt
     2881 tttacttcgt gaactttgtg gccccgaagg ggctcgtggt gaaatggatc tttctttaag
     2941 gagctgacat ggacttcgcg ccggccgatc ggttgaaaaa actgccgcct tacctcttca
     3001 tcgagatcga caagaagaaa aaagccgcga tcgagaaggg ggtggacatc atcagcctgg
     3061 ggatcggcga ccccgacctg gccacgccgg acttcatcat cgaggccctc tataaggccg
     3121 ccaaggacaa ggccaaccac caatatcccc tgggcacggg cctgcccaag ttccgcgagg
     3181 ccgtggccaa gtggtaccag cgccgcttca acgtcacgct ggaacccaag accgaagtgg
     3241 tggccctcat cggctccaag gaaggcatcg gccacctgcc cgtcggcgtg ctcaaccccg
     3301 gcgacgtctg cctggtgccc tcgcccggat acccggtcta ccacgcgggc acgctcttcg
     3361 cgggcggcga gtcctatttc atgcccctgc tcgaaaagaa cggctttttg cccgacctgg
     3421 acgccatccc ggcggaggcg ctcaaacgcg ccaagctcat ctggaccaac tcgcccaaca
     3481 accccacctc cgccctggcg gggaaggact tctacgccaa ggtgatcgag ttcgccaaga
     3541 aacaccgcct gatcgtggcc aatgacgccg cctacaccga gatctactac gacggcaaga
     3601 aacccgtcag cttcc
//
LOCUS       DASTJJ010000615         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_208124, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000615 DASTJJ010000000
VERSION     DASTJJ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208124"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1109
                     /locus_tag="VFR02_07525"
     CDS             <1..>1109
                     /locus_tag="VFR02_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9870327.1"
                     /translation="MAPSKLVSPGTEVDVAVLEERRAEERTSLVASLQEKQVVEGVVK
                     NITDYGAFVDLGGVDGLLHVTDIAWRRVSHPTEVLSVGQTVTVQIIKINHETQRISLG
                     MKQLQTDPWEGVAAKYPVGAQFKGKVTNVTDYGAFVELEPGVEGLVHVSEMSWVKKNM
                     APSKLVSPGTEVDVAVLEVDSNKRRISLGLKQTQENPWSAFTAGHSIGSVIEGEIKSI
                     TEFGLFIGLDTEIDGMVHLSDLSWSQPGDEAIKTYQKGQMVKAKVLEIDVEKERVSLG
                     IKQLEADPIDSAGAGGAPLRKGKVVTGTITEVNDGGIEVELEGGVRSFIRRADLARDR
                     GDQRPDRFGKGEKIDALVTSIDKNSRKVSLSIKAR"
BASE COUNT          228 a          351 c          361 g          169 t
ORIGIN      
        1 atggccccct ccaagctggt cagccccggc accgaggtgg acgtggccgt gctggaagaa
       61 cgccgtgccg aggaacgcac ctcgctggtg gcctcgctcc aggaaaagca ggtggtcgaa
      121 ggcgtggtca agaacatcac cgactatggc gccttcgtcg acctgggcgg ggtggacggg
      181 cttctgcacg tcaccgacat cgcctggcgg cgcgtgagcc acccgaccga agtcctgtcg
      241 gtgggccaga ccgtgaccgt gcagatcatc aagatcaacc atgagaccca gcgcatctcg
      301 ctgggcatga agcagcttca gaccgatccc tgggagggcg tggcggccaa atatccggtg
      361 ggcgcgcaat tcaagggcaa ggtcaccaac gtcaccgact atggcgcctt cgtggagctg
      421 gagccgggcg tcgagggcct ggtccatgtc tcggaaatgt cctgggtgaa gaagaacatg
      481 gccccctcca agctggtcag ccccggcacc gaggtggacg tggccgtgct ggaagtcgat
      541 tccaacaagc gccgcatctc gctgggcctg aagcagaccc aggaaaaccc ctggagcgcc
      601 ttcaccgcgg gccattcgat cggcagcgtc atcgagggcg agatcaagtc gatcaccgaa
      661 ttcggcctgt tcatcggcct ggataccgag atcgacggca tggtccacct gtccgacctg
      721 tcctggtccc agcccggcga cgaggccatc aagacctatc agaagggtca gatggtcaag
      781 gccaaggtgc tggaaatcga cgtggaaaag gaacgggtct cgctgggcat caagcagctt
      841 gaagccgacc cgatcgattc cgccggcgcc ggcggtgccc ccctgcgcaa gggcaaggtg
      901 gtcaccggca ccatcaccga ggtcaatgac ggtggcatcg aagtggaact ggaagggggg
      961 gtacgctcct tcatccgccg ggccgacctg gcccgcgacc gcggcgacca gcgccccgac
     1021 cgtttcggca agggcgagaa gatcgatgcc ctagtcacct ccatcgacaa gaacagccgc
     1081 aaggtctcct tgtccatcaa ggccaggga
//
LOCUS       DASTJJ010000616         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_208695, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000616 DASTJJ010000000
VERSION     DASTJJ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208695"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFR02_07530"
     CDS             complement(<1..586)
                     /locus_tag="VFR02_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870328.1"
                     /translation="MILGIDGTCMQGARTGAGWYLTHLLEALSHHLEEDKVIVWMNSP
                     TPEERSRVPENRFISVNATHYPWTALKMTWNTLGTPSMETLLGRPADVCFYPNYLPLP
                     QKGGKQALFVHDLAYLTNPEWGQGTDFKHFKRNIEKQEEKVDLILTCSEFNRQELTRQ
                     FAGLSDAKVRVIPHGLSKEFARPAPAERVQKVKAH"
     gene            complement(623..>1674)
                     /locus_tag="VFR02_07535"
     CDS             complement(623..>1674)
                     /locus_tag="VFR02_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870329.1"
                     /translation="TGRLRHPEALVFLLGGFLGSALNFGVFAVLYRLLGVHPTLAFFV
                     STFLNEAFHHVYYRTVYVNEEIQFRTPGAIQWFLYVWTALGSALLLTGFHQWLGWDVW
                     LAALATLAILSVSNTLFVRISTFGSAHLAEVEYLEMNESYYDDQTNEEKVSKFRAWYH
                     RSRYEKLTRFVESHYRKGMKVADLGCGNCWWNVHALPVTGVDINPKMLGWAKKNKRLK
                     DFKVTGNLGKTGLPSASFDLVIMTETLEHILDLPGVLREVKRILKPGGTFLITVPYDI
                     FLGPFFILFNLNCFYMGYFRGSQYHKYRCGHINHFSKSRLARTLSENGFEMKSSFLVN
                     FLLLYAVARKAGAGK"
BASE COUNT          324 a          487 c          545 g          318 t
ORIGIN      
        1 aatgggcctt gaccttttgg acccgctcgg cgggcgcggg ccgggcgaat tccttggaga
       61 ggccgtgggg gatgacccgg accttggcgt ccgagagccc cgcgaattgc cgggtgagct
      121 cttggcggtt gaactccgag caggtgagga tcaggtccac cttctcttcc tgcttctcga
      181 tgttgcgctt gaaatgcttg aagtccgtcc cctggcccca ttccggattg gtcaggtagg
      241 ccaggtcgtg cacgaagagg gcctgtttgc cgcccttctg gggcaagggc aggtagttgg
      301 gataaaaaca gacgtccgcg ggacggccca gcagggtctc catggaaggg gtccccaagg
      361 tgttccaggt catcttgagc gcggtccagg gatagtgggt ggcgttgacc gagatgaacc
      421 ggttctcggg cacccgggac ctttcctcgg gggtcgggct gttcatccag acgatgacct
      481 tgtcctcttc caggtggtgg gacaaggcct ccaacaaatg ggtcaagtac catcccgcgc
      541 cggtgcgggc gccctgcatg caggtgccgt cgattccaag gatcacgcga aaacccgcct
      601 cacgagtggg atgccgccgg gctcacttcc ccgcgccggc cttgcgggcc acggcgtaca
      661 acaacaggaa gttcaccaag aaggagcttt tcatctcgaa cccgttctcc gaaagcgtgc
      721 gggccaggcg gctcttgctg aagtggttga tgtgtccaca ccggtatttg tggtactggc
      781 tgccccggaa atagcccatg taaaagcagt tcaggttgaa aaggatgaag aaggggccta
      841 ggaaaatgtc atagggcacc gtgatcagga aggtaccgcc cggtttcagg atgcgtttga
      901 cctcccgcag gacccccggc aggtccagga tatgctccag ggtctcggtc atgatgacca
      961 ggtcgaagga agcggacggc agcccggtct tgcccaggtt ccccgtgacc ttgaaatcct
     1021 tcagccgctt gttctttttc gcccaaccca gcatcttggg gttgatgtcc accccggtca
     1081 ccggcagggc gtgcacgttc caccagcagt tgccgcagcc caggtcggcc accttcatgc
     1141 ccttgcggta gtggctttcc acgaagcggg tcagtttctc gtagcgggag cggtggtacc
     1201 aggcacggaa cttgctgacc ttctcctcgt tggtctgatc gtcgtaatag ctctcgttca
     1261 tctccaggta ttccacctcg gccaggtggg ccgacccgaa ggtcgagatg cgcacgaaga
     1321 gggtgttgga caccgagagg atggccaggg tcgccaaggc ggccagccac acgtcccacc
     1381 ccaaccattg atggaagccg gtcagcagga gggcgctgcc cagggccgtc cagacgtaaa
     1441 ggaaccattg gatggcgccg ggggtgcgga attggatctc ctcgttgacg tagaccgtgc
     1501 ggtaatagac gtggtggaag gcctcgttga ggaaggtgct cacgaaaaag gccagggtgg
     1561 gatgcacgcc caaaagccgg tagagcacgg cgaagacccc gaaattcagg gcgctgccca
     1621 gaaaaccgcc cagtaggaag accagcgcct cgggatgccg caagcggccc gtca
//
LOCUS       DASTJJ010000617         1748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_818134, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000617 DASTJJ010000000
VERSION     DASTJJ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818134"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="sucD"
                     /locus_tag="VFR02_07540"
     CDS             complement(<1..741)
                     /gene="sucD"
                     /locus_tag="VFR02_07540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9870330.1"
                     /translation="MSVFLDKKSKVICQGITGSAGLFHSKACREYGTKLVGGVTPGKG
                     GSEIEGFQVFDTVAEAVAKTGANTTMIFVPALFAADAILEAAAAGIALIVCITEGIPA
                     NDMARVNEVLKRYPHSRLIGPNCPGIITPGVAKVGIMPGYIHKPGKIGIVSRSGTLTY
                     EAVWQVTQLKLGQSTCIGIGGDPIVGTTHLDALAAFNEDKDTQAVIMIGEIGGTAEEE
                     AARYVKKEMKKPVAAFIAGTTAPPGKRMG"
     gene            complement(738..>1748)
                     /gene="sucC"
                     /locus_tag="VFR02_07545"
     CDS             complement(738..>1748)
                     /gene="sucC"
                     /locus_tag="VFR02_07545"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9870331.1"
                     /translation="GKAGGVKLAKTEADLRQRASDILGMTLISPQTGPEGRLVKKIFI
                     TPALDYVKELYVSVVLDRGARCPVVLASAEGGTEIEELAATKPEAIQKTFVNPLQGLE
                     PYQARELYLGLGLPKERINAGAKFFQDLVKMFLELDLSLLEVNPLVVLKNGEMHCLDA
                     KVAFEDNGLARHKDYEAWRDADEEDARELEAGKSGLSYISLDGNIGCLVNGAGLAMAT
                     ADAISFFGGSPANFLDVGGGATTKAVTAAFKIILSDERVKAILVNIFGGIMKCDVIAQ
                     GIIDAAKDVGLALPLVVRLEGTNVEQGKALLAKSDLKYKFASSMDEAAKAVVAAAKEA
                     RS"
BASE COUNT          251 a          589 c          579 g          329 t
ORIGIN      
        1 gcccatgcgc ttgcccgggg gggccgtggt gcccgcgatg aaggccgcca cgggtttctt
       61 catttctttc ttcacgtagc gggcggcctc ctcctcggcg gtgccgccga tctcgccgat
      121 catgatgacc gcctgcgtgt ccttgtcctc gttgaaggcc gccagggcgt ccaggtgggt
      181 ggtgcccacg atggggtcgc cgccgatgcc gatgcaggtg gactggccca gcttcaattg
      241 ggtgacctgc cagaccgcct cataggtgag ggtgcccgag cgggacacga tgccgatctt
      301 gcccggtttg tggatgtagc ccggcatgat gcccaccttg gccacgcccg gcgtgatgat
      361 gcccgggcag ttgggcccga tgagccggct gtggggataa cgcttcagca cctcgttgac
      421 ccgggccatg tcgttggccg ggatcccctc ggtgatgcac acgatgaggg cgatgcccgc
      481 cgccgcggct tccaggatgg cgtcggccgc gaagagcgcg ggcacgaaga tcatggtggt
      541 gttggcgccg gtcttggcca cggcctcggc cacggtgtcg aacacctgga acccctcgat
      601 ctcggatccc cccttgcccg gggtgacgcc ccccaccagc ttggtgccgt attcccggca
      661 ggccttggaa tggaacaacc ccgccgagcc cgtgatgccc tggcagatga ccttggactt
      721 cttgtcgagg aacacgctca tgaccgggcc tccttggccg cggccaccac ggccttggcc
      781 gcctcgtcca tgctggaggc gaatttgtat ttcaggtccg acttggcgag caaggccttg
      841 ccctgctcca cgttggtgcc ttccaggcgc accaccaggg ggagcgccag ccccacgtcc
      901 ttggccgcgt cgatgatgcc ctgggcgatg acgtcgcact tcatgatgcc gccgaagatg
      961 ttgaccaaaa tggccttcac ccgctcgtcg gacaggatga tcttgaaggc cgccgtgacc
     1021 gccttggtgg tggccccgcc gcccacgtcc aggaaattgg cgggcgaccc gccgaagaag
     1081 gagatggcgt ccgcggtggc catggccagc ccggcgccgt tgaccaggca gccgatgttg
     1141 ccgtccagcg agatgtagga aagcccgctc ttgcccgctt ccagttcccg ggcgtcctcc
     1201 tcgtcggcgt cgcgccaggc ctcgtagtcc ttgtgccggg ccaggccgtt gtcctcgaag
     1261 gccaccttcg cgtctaggca atgcatctcg ccgttcttca ggaccaccag ggggttcact
     1321 tccagcagcg acaggtccag ttccaggaac atcttgacca ggtcctggaa gaacttggcg
     1381 cccgcgttga tgcgttcctt gggcaggccc agccccaggt aaagctcccg ggcctggtag
     1441 ggctccagcc cctgcagggg gttcacgaag gtcttttgga tggcctccgg cttggtggcg
     1501 gccagttcct cgatctcggt gccgccctcg gcggaggcca gcaccacggg gcagcgggcg
     1561 ccccggtcca gcaccacgga cacgtagagt tccttcacgt aatccagcgc cggcgtgatg
     1621 aagatcttct tgaccaggcg cccttcgggg ccggtctggg gggaaatgag ggtcatgccc
     1681 aggatgtcgc tggcgcgctg gcgcaggtcg gcctcggtct tggccagctt cacgccgccc
     1741 gccttgcc
//
LOCUS       DASTJJ010000618         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_330870, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000618 DASTJJ010000000
VERSION     DASTJJ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330870"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..>1683)
                     /locus_tag="VFR02_07550"
     CDS             complement(93..>1683)
                     /locus_tag="VFR02_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET9870332.1"
                     /translation="KSIVCLRGYSWKQFRDDSAAGAVVGLVALPLAMAFAIASGLPPE
                     RGIYTAVVAGFLISALSGSRVAIGGPTGAFIVVVAGITARYGYEGLAQATFLAGILLA
                     LLGLARVGTWVKYMPYPVVTGFTTGIAVVIFSTQVRDLLGLPPGPREGFLAQWGDCLR
                     SLPGWNPYALGTGLFTAAAIFLWPRGWKVPGSIAALAVSASAAAWGHWPLETIGSRFG
                     GIPRGLPHPQFHFPDAHQALVLLPAAATVAALGAVESLLCAVVADGMIGGRHKSNLEL
                     VAQGAANFLSPVFGGMPATGAIARTATNVRNGGRTPVAGMVHALVLLLILLTAGPWAA
                     RIPLAALAGVLVPVCWHMAEWHSCRFILRGPWADAAVLGLTFFLTLFVNLSVAVGAGL
                     ALALAVHARNRAFHGKGKPVREPGPRPEAPAGVEVYAPEGALSLGSAERLVEVERALF
                     PAPRAFVLDMEGVFYMDATGLKVLKDIRERCAARGTRFIVAGLQPQPLSMLRKAGKLE
                     KIGRENLERGLRQALAGLAGG"
BASE COUNT          291 a          622 c          577 g          193 t
ORIGIN      
        1 ctttcacggg tccctccggg gcgatggatg gcgtcgggag tttggacgtc gccccggcgg
       61 cggatgcctc gaaaaatcgt ccgggggatg ggtcaccccc ccgccaggcc cgccaaggcc
      121 tgccgcaggc cgcgctcgag gttctcccgg ccgatctttt ccaatttacc ggccttgcgc
      181 agcatcgaga ggggttgggg ttggagcccc gccacgatga accgggtccc gcgggccgcg
      241 cacctttccc ggatgtcctt caacaccttc aggcccgtgg cgtccatgta aaagacgcct
      301 tccatatcca gcacgaaggc ccggggggcc gggaaaagcg cccgttccac ctccaccaac
      361 cgctcggcgg aaccgagcga aagggccccc tcgggggcat agacctccac gcccgcgggc
      421 gcctcgggcc gggggcccgg ttcccggacc ggcttcccct tcccgtggaa ggcccggttg
      481 cgcgcgtgga ccgccagcgc cagggccaac cccgcgccca cggccaccga caggttcacg
      541 aagagggtca ggaaaaaggt caggccgagg acggcggcgt ccgcccaggg accgcgcagg
      601 atgaagcggc aggaatgcca ttcggccatg tgccaacaga ccggcaccaa cacgcccgcc
      661 agggcggcca gcgggatccg ggcggcccag ggcccggccg tcaggaggat gagcagcagc
      721 accagcgcgt gcaccatgcc cgccaccggg gtccgtcccc cgttgcggac attggtggcc
      781 gtgcgggcga tggcccccgt ggcgggcatg cccccgaaga ccggggacag gaaattggcc
      841 gccccttggg ccaccagctc caggttggac ttatggcgcc cgccgatcat cccatcggcc
      901 accacggcgc aaagcagcga ctccacggcc cccaaggccg ccaccgtggc ggccgccggc
      961 agcaggacca aggcctggtg cgcgtccggg aaatggaact ggggatgggg caggccccgg
     1021 gggatccccc cgaaccggct gccgatggtc tccaggggcc aatgccccca ggccgcggcc
     1081 gacgccgaga ccgccagggc cgcgatggac cccgggacct tccagccccg gggccaaagg
     1141 aagatggcgg cggcggtgaa gagcccggtc cccagggcgt aggggttcca tccgggcagg
     1201 gaccgcaggc agtcccccca ttgggccagg aacccctccc ggggcccggg cggcaggccc
     1261 agcaggtccc ggacctgggt gctgaagatg accaccgcga tgccggtggt gaagcccgtg
     1321 accaccggat agggcatgta tttcacccag gtccccaccc gcgccaggcc caggagcgcc
     1381 aacaggatgc ccgccaggaa ggtggcttgg gccagcccct cataaccgta tcgggccgtg
     1441 atgcccgcca ccaccacgat gaaggcgccc gtgggcccgc cgatggccac ccggctgccg
     1501 ctcaaggccg agatcaggaa ccccgccacc accgccgtat agatcccccg ctcggggggc
     1561 agcccggagg cgatggcgaa ggccatggcc aagggcaggg ccaccaggcc caccaccgcg
     1621 ccggcagccg aatcgtcccg gaactgtttc caggaatagc ccctcaggca aacaatggat
     1681 ttc
//
LOCUS       DASTJJ010000619         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1430867, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000619 DASTJJ010000000
VERSION     DASTJJ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430867"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1076)
                     /locus_tag="VFR02_07555"
     CDS             complement(<1..>1076)
                     /locus_tag="VFR02_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9870333.1"
                     /translation="FVMLLRSSGRTAWLGWRLAGLVILFALVLTRVRGAEIAAAVGFL
                     FLVAAFALPWGRDLFQRNRRQLWGLLGVLVVAGALTLFWKGDAGWAGAGASLAQREAI
                     YRTAWEIVKDHPWTGVGLGQVGAVYPAYQYRPYAPQDYADHPYVYTEHVHDEYLQFWV
                     EGGLAGLLLFLAVLAAFALAVRKALKSPATTAEGRELLLGVCAGMTALLTQALSNFPF
                     QVAPTAVLFGLFLAAPLALRPPIPVPPGRLDPAKTWALGLALLLGAAGGIRILAGTIA
                     ARDTAGENSLGHAQKAVYYAGRLTTLLPWSPKAWNLAGQALGQAGQGDAAAKAYERSL
                     ALNPGYVENFMALADLRARAGDLP"
BASE COUNT          199 a          389 c          358 g          130 t
ORIGIN      
        1 tggggcaggt cgcccgcccg ggcccgcagg tccgccaggg ccatgaaatt ctcgacgtat
       61 ccggggttca aggccagcga cctttcataa gccttggccg ccgcgtcccc ctggcctgcc
      121 tgccccaggg cctggcccgc caggttccag gccttggggc tccaaggcag gagggtggtc
      181 aggcggccgg cgtagtaaac ggccttttgg gcgtgaccca gggaattttc cccggcggtg
      241 tcccgggcgg cgatggtccc tgccaggatg cgtatccccc cggcggcgcc cagcaagagc
      301 gccaagccca gggcccaggt cttggccgga tccaaccggc ccgggggaac ggggatgggg
      361 ggacgcagcg ccagcggcgc cgccaggaag agcccgaaga gcaccgccgt gggcgccact
      421 tgaaagggaa aattgctcag ggcctgggtc agcagcgcgg tcatccccgc gcagacgccc
      481 aacagcagtt cccgcccctc ggccgtggtc gctggggact tcagggcctt gcggaccgcc
      541 agcgcgaagg ccgccaagac cgccaagaac aacaacagcc ccgccagccc gccttccacc
      601 cagaattgca ggtactcgtc atgcacatgc tcggtataga cataaggatg gtcggcatag
      661 tcctggggcg cgtagggccg gtactggtag gccggataga ccgcccccac ttggcccaat
      721 cccacgcccg tccagggatg gtccttcacg atctcccagg ccgtgcggta gatggcttcc
      781 cgctgggcca gggatgcccc cgcgcccgcc caaccggcgt cccctttcca gaacagggtc
      841 aaggcgccgg ccaccaccag gacgcccaag agcccccaaa gctggcggcg gttgcgttgg
      901 aaaaggtccc gcccccaagg cagggcgaag gccgccacga ggaaaaggaa gcccacggcg
      961 gcggcgatct ccgcgccccg gacccgggtc aggaccaacg cgaagaggat caccaagccg
     1021 gccaggcgcc aacccagcca ggccgtccgg cccgaggacc gcaacaacat cacgaa
//
LOCUS       DASTJJ010000620         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_209018, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000620 DASTJJ010000000
VERSION     DASTJJ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209018"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFR02_07560"
     CDS             <1..1017
                     /locus_tag="VFR02_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET9870334.1"
                     /translation="VPGATYLINTATLTYSGLATPKTVTANVEVSANYLVHLAVYNSV
                     GELIKQILIQTYSQPMDSIQLEQTQEITSLNGTVYVIVNGQAIASWNGTNQAGDPVSN
                     GQYYIKLDSQDPYGVVTSVSQPVVVNRILTKVEVDIFNSVGEIVRHLYNYVNDPDGTE
                     MSDIKLSSAVLNPNNTSGMGAPGQVQVEVETNGSGSGVSLTWDGRSDGGQVVANGQYM
                     IEARWTDSNGGSLVDTKDIVVLGSGNSPAQGKVYASPQILSSGDPVTKLQINSPLALT
                     FHVNVYDVAGELLKNQKVVQGTQSATWDSSGVASGVYILVVTLTDPSGQFVDRQTVKV
                     VVLH"
     gene            1093..1668
                     /locus_tag="VFR02_07565"
     CDS             1093..1668
                     /locus_tag="VFR02_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870335.1"
                     /translation="MKPSLILSFCMAAGLSAWAPAWSDQEPLSLQDLGFSAQDTTADP
                     QFQKQLEIRSDMLHVHQILGLVTAVPMVTTYVLGLTTADNVEKGGTDTQLHAALGLTT
                     TGLYLTTASFAIFAPKPEGLKPSGSTQIHETLAWIHGPLMIITPLLGDMVDDRVQNHQ
                     PLGALGTIHAVAATALVTSYLASLLVETLNL"
     gene            1771..2259
                     /locus_tag="VFR02_07570"
     CDS             1771..2259
                     /locus_tag="VFR02_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9870336.1"
                     /translation="MITYHVTHPLHEAEGKSTAARGKGSVSYAGSGDFLVAVPVKTFD
                     SGDSNRDLHMWEITRAGTHPMVVVNVKIKGTSVAAFPAVLTAEATVDFAGKEKTYSLP
                     LTVRSDGPETLALTGSVPLSLKDFDIQAPSLLAMPIRDDVPVGLDMVWRRTGTPAAAD
                     KK"
BASE COUNT          462 a          736 c          709 g          431 t
ORIGIN      
        1 gtgccgggcg ccacctacct gatcaatacg gcgaccctga cctactcggg cttggccacg
       61 cccaagaccg tgaccgcgaa cgtggaggtg tcggccaact acctggtgca tctggcggtg
      121 tataactcgg tcggcgagct catcaaacag atcctgatcc agacctattc ccagcccatg
      181 gacagcatcc agttggaaca gacccaggag atcaccagcc tgaacggcac ggtctacgtc
      241 atcgtcaacg ggcaggccat cgcgtcctgg aacgggacca accaagccgg ggacccggtc
      301 tccaacgggc agtattacat caaactggac agccaggacc cctacggcgt ggtgaccagc
      361 gtgtcccagc ccgtggtggt gaaccgcatc ctgaccaagg tggaagtgga catcttcaac
      421 tcggtcgggg agatcgtccg gcatctttat aactacgtga acgaccccga cggcaccgag
      481 atgtccgaca tcaagctttc cagcgcggtg ctcaacccca acaacaccag cgggatgggc
      541 gcccccggcc aggtgcaggt ggaagtggag accaacggtt cgggttcggg cgtcagcctg
      601 acctgggacg gccgctcgga cggcggccag gtggtcgcga acgggcagta catgatcgag
      661 gcccgctgga ccgacagcaa cggcggcagc ctggtggaca ccaaggacat cgtggtcctg
      721 ggctccggga acagcccggc ccagggcaag gtttacgcga gcccgcagat cctgtccagc
      781 ggggacccgg tgaccaagct ccagatcaat tcgccgctcg ccttgacctt ccatgtcaat
      841 gtctacgacg tggccggtga actgctaaag aaccagaagg tggtccaggg cacccagtcg
      901 gcgacctggg attcctccgg cgtggccagc ggcgtctata tcctcgtggt gaccctcacc
      961 gatcccagcg gccagttcgt cgaccgccag acggtcaagg tcgtggtcct gcactgaccg
     1021 ccggcggtcg gggggcttga acttttccgg ggaaaggggt attttcccgg catcccgtgg
     1081 aaaaggaccc cgatgaaacc ctccctcatt ctttccttct gtatggcggc cgggctatcg
     1141 gcctgggcgc cggcctggtc cgatcaggag cccctgtccc tccaggacct cggtttcagc
     1201 gcccaagaca ccaccgccga tccccagttc caaaaacagc tcgagatccg ttccgacatg
     1261 ctgcacgtgc accagatcct gggattggtg acggcggtcc cgatggtcac cacctacgtg
     1321 ttgggcctga ccaccgcgga taacgtggaa aaagggggga ccgacaccca gctccacgcg
     1381 gccttgggcc tcactaccac ggggctttac ctcacgacgg cttccttcgc gatcttcgcc
     1441 cccaaacccg aggggctgaa accctccgga tcgacccaga tccatgagac cttggcctgg
     1501 atccatgggc cgctcatgat catcacgccg ctgttgggcg acatggtcga tgaccgcgtc
     1561 caaaaccacc agcctttggg ggccctgggg accatccacg cggtggccgc caccgccttg
     1621 gtgacgtcct atttggcctc tttattggtg gaaaccctga acctgtagga ggaagctatg
     1681 cggtggtggc tggcccttgt ttttctcctt tgttttccag gcttttccgg cgctttcgat
     1741 ctgcgcggct cttggatatt ggaaaagtcc gtgatcacct accacgtcac ccacccgctc
     1801 catgaggccg agggaaaaag tacagccgcc cgggggaaag gtagtgtatc ctatgccgga
     1861 tcaggcgatt tcctggtggc cgtgccggtg aaaaccttcg attcggggga ttcgaaccgg
     1921 gacctgcata tgtgggagat cacccgggcg ggaacccatc cgatggtggt ggtgaacgtt
     1981 aaaatcaagg ggacgagcgt cgcggccttc ccggcggtct tgaccgctga agcgaccgtg
     2041 gatttcgcgg gcaaggaaaa gacctattcc ttgccgttga ctgtacggtc ggacgggccg
     2101 gaaaccctgg ccttgaccgg gtccgtgccg ctctccttga aggatttcga cattcaagcg
     2161 cccagcctgc tggccatgcc cattcgggac gatgtgccgg tgggcctgga catggtttgg
     2221 cggcggacgg ggaccccggc ggccgcggat aaaaaataaa aaggacccgt ttcatggaac
     2281 aagttctcgt tcaagacagc ttcgccaagg ctcaagaacc caagacggag cagctttt
//
LOCUS       DASTJJ010000621         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_573963, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000621 DASTJJ010000000
VERSION     DASTJJ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573963"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFR02_07575"
     CDS             complement(<1..353)
                     /locus_tag="VFR02_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9870337.1"
                     /translation="MTRPEFVHLHLHSQYSLLESTVRLEPLMARVKELGMPAVALTDK
                     ANLFGAIHFYQAALAHGIKPILGCELLVAPRSRFDKTHAHGRPEAAYHLVVLAENEEG
                     YHNLMKLSTAGYLQG"
     gene            complement(408..758)
                     /locus_tag="VFR02_07580"
     CDS             complement(408..758)
                     /locus_tag="VFR02_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET9870338.1"
                     /translation="MGRNFPGVGPLLAGFFSTSGPLRAEGSGSVRTTTFESVELGNPW
                     QTVLFNDEVHSFDEVILQIQKATGFPLERAAQITLRVHHNGKAIVTIGAKAECERVAA
                     ILGQIQLTTSVEKT"
     gene            915..1463
                     /locus_tag="VFR02_07585"
     CDS             915..1463
                     /locus_tag="VFR02_07585"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HET9870339.1"
                     /translation="MGLTATLYRFTLDLSDVDRALYRTLEFRAALHPSESLPFFVTRI
                     LAYALNHREGLEFSAGISAPDEPAIRVPGPTGGIDLWIDIGNPAAKRIHKASKSAKEV
                     RIYTHKDAQLLAREFKAEGVHKLESIGLYSLEPKFLNALGAALDRDNHWEITRNEGEL
                     YVGVGKETFQGRLTHHPLSGPA"
     gene            complement(1495..>1928)
                     /locus_tag="VFR02_07590"
     CDS             complement(1495..>1928)
                     /locus_tag="VFR02_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870340.1"
                     /translation="TVLIVEDQKGFRRVYRDVLESAGLRVLDAADGEAGLEQVRLYRP
                     GLVLLDVGLPGLNGHEVLRRIREDASLDGVKVVLFSVMGEEPEVQRGLALGADGYALK
                     GGHSPKQVVAQVLELLKAPGQRPIPGLPVESGKGPAESPGP"
BASE COUNT          357 a          646 c          613 g          312 t
ORIGIN      
        1 tagccctgca ggtagcccgc ggtggagagc ttcatcaggt tgtggtagcc ctcctcattc
       61 tcggccagca ccaccaggtg gtaggccgcc tcggggcggc cgtgggcgtg ggtcttgtcg
      121 aaacgggacc ggggggccac caacagctcg cagcccagga tgggcttgat gccgtgggca
      181 agcgccgcct ggtagaaatg gatggccccg aaaaggttgg ccttgtcggt caaggccacg
      241 gccggcatcc ccagctcctt gacccgggcc atcaggggct ccaggcgcac cgtgctttcc
      301 agcaggctgt attgggaatg gaggtggagg tggacgaact cgggcctggt catgggccca
      361 ttataggggg gcggcgggag gtttgacgag gcggaagggg cgggccccta ggtcttttcc
      421 accgaggtgg tgagctggat ctggcccagg atggccgcca cgcgctcgca ttcggccttg
      481 gcgccgatgg tcacgatggc cttgccgttg tggtgcaccc gcagggtgat ctgggcggcc
      541 cgctccaggg ggaaccccgt ggctttctgg atctgcagga tgacctcgtc gaaactgtgg
      601 acctcgtcgt tgaacagcac ggtctgccag gggttgccca gctccacgga ctcgaaggtc
      661 gtcgtccgga cggaaccgga cccctcggcc cgaagcggtc ccgacgttga aaagaacccg
      721 gccaacagcg gaccgacccc cgggaaattc cggcccataa gatcccccag agaagaagtt
      781 gaaataaccc ggcgattata acgaaaaggc ccgttccaag gccagcttcg ggggaagaaa
      841 gggaaaaccc cgcggcccgg gagacacccg gcctggttcc ttatagaatg accgaaaacc
      901 gccccggagg acccttgggc ctgaccgcca ccctttaccg cttcaccctg gacctgtcgg
      961 acgtggaccg cgccctttac cggaccttgg agttccgggc cgccctgcac ccatccgagt
     1021 ccctgccctt tttcgtcacc cggatcctgg cctacgcctt gaaccaccgg gaaggcctgg
     1081 aattttccgc cgggatcagc gcccccgacg agcccgccat ccgggtcccg ggccccaccg
     1141 gcggcatcga cctttggatc gacatcggca atcccgcggc caaacgcatc cacaaggcgt
     1201 ccaaatcggc caaggaggtc cgcatttaca cccacaagga cgcccagttg ctggcccggg
     1261 aattcaaggc ggagggcgtc cacaagctgg agtccatcgg cctctattcg ctggaaccga
     1321 aattcctcaa cgccctcggc gccgccctgg accgggacaa ccactgggag atcacccgca
     1381 atgaaggcga gctttatgtg ggggtgggga aagagacctt ccaggggcgg ttgacccatc
     1441 atcccctaag cgggccggcc tgaaagcgga tggatcgatg gaaaaagggg accttcaggg
     1501 ccccggggac tcggcggggc ccttccctga ctccactgga aggccgggaa tgggcctttg
     1561 gccgggtgct ttcaggagtt ccaggacctg ggcgacgacc tgcttggggg aatggccgcc
     1621 cttgagggcg tagccgtcgg cgcccagggc caaaccccgt tggacctccg gttcctcgcc
     1681 catcactgaa aaaaggacca ccttgacgcc gtccaatgac gcgtcctccc ggatgcgccg
     1741 cagcacctcg tggccgttga ggcccggcag gcccacgtcc aaaaggacca accccggccg
     1801 gtagagcctg acctgctcca accccgcttc cccgtcggcg gcgtccagca cccgcaaccc
     1861 cgcgctttcc aacacgtcgc ggtacacccg ccgaaaaccc ttttggtcct ccacgatcaa
     1921 tacggtcg
//
LOCUS       DASTJJ010000622         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_574038, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000622 DASTJJ010000000
VERSION     DASTJJ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574038"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFR02_07595"
     CDS             complement(<1..295)
                     /locus_tag="VFR02_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9870341.1"
                     /translation="MAKKKRPSLRAKNSHPKTGPGILNAKTAILCATSIVIALVVGLV
                     ALGLIRLIAFFTNLSFYHRLSFETVSPADNGLGLLVILVPVVGGVIVGLMACWG"
     gene            480..806
                     /locus_tag="VFR02_07600"
     CDS             480..806
                     /locus_tag="VFR02_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870342.1"
                     /translation="MFAVAAMGGLILAVLHFKKKHLPLPLALLHGLLGAAGLVTLLVF
                     LLQGGTASKVALSLGLFVVAALGGFFLFSFHLRKKPLPSPVVIIHALVAVAAFILLLL
                     AAFFPA"
     gene            838..1263
                     /locus_tag="VFR02_07605"
     CDS             838..1263
                     /locus_tag="VFR02_07605"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9870343.1"
                     /translation="MGHPLHAPFTHFPLALWVAAFLGDLVHAGWGGEFWRAFSFWNIA
                     FGMGFGIVTATTGFYDLMKIPDGKTEALRTGIFHMGVMLAAFCLFGASLYFHREAPAA
                     EPWRPLPGLLLSGSGTLVLLFGGWLGGQLVYRHGVGYDR"
BASE COUNT          326 a          465 c          519 g          414 t
ORIGIN      
        1 gcccccaaca ggccatgagt cccacgatga cgccgccgac tactgggacc aggatgacga
       61 ggagaccgag gccgttgtcc gccggggaga cggtttcgaa ggagaggcgg tggtaaaagg
      121 aaaggttggt gaagaaggcg attaaacgga tcaatcccag ggctaccagc ccaaccacca
      181 gggcgataac tatggaggta gcgcaaagga ttgcggtttt ggcgttcaag atgcccggac
      241 cggtcttggg gtgggagttt tttgcccgga gggacggtct ttttttctta gccaaaactc
      301 ctcctttgga tcgacttgga atgtcccgag tttacgggaa cgaccccgaa aattgcaacg
      361 gacgatagga actaagccta ataaaacaag tttttacttg ttttattaaa gcgggccctt
      421 tatagtctgg aaagatcttt gattacaaag gaggccttat gcttttgatc gctgtcgggt
      481 tgttcgccgt cgccgctatg ggtggtttga tcctggcggt tctccatttc aagaagaagc
      541 acctccccct tccattggcc ttgctccacg ggctcttggg agcggcgggc ttggtgacct
      601 tgctggtctt cctcttgcag ggagggaccg cttcgaaagt cgctctttcg ctcggcttgt
      661 tcgtggtggc ggctttggga ggcttcttcc tgttctcctt tcacctgagg aaaaagccgt
      721 taccgagccc ggtcgtgatc atccacgccc tggtcgccgt tgccgctttc atcctgctcc
      781 tgttagcggc ctttttcccg gcctgaaatg acccatcggg aagacgcttt caggccgatg
      841 ggtcatccgc ttcacgctcc ttttacccat tttccgttgg ccttgtgggt ggcggccttc
      901 ctgggggacc tggtccatgc cgggtgggga ggggagtttt ggcgggcctt ctctttttgg
      961 aacatcgctt ttggcatggg cttcgggatc gtgacggcca caaccggttt ttacgacctg
     1021 atgaagatcc cggacggcaa gaccgaggcc cttcggacgg ggattttcca catgggggtc
     1081 atgctggcgg ccttttgtct ttttggggcc agcctttatt tccataggga agctcccgcc
     1141 gccgagccgt ggcggcccct gcccgggctt ttgctttcgg gatcggggac cctggtcctt
     1201 ctcttcggcg gctggctggg aggacagttg gtttaccggc atggcgtagg ctatgatcga
     1261 taacaacatg aacatcaggc cccacggatg aaattagaga atctttccca ctccaacagg
     1321 tccatcctcc tgttattgaa aagacagggg gccttgacca tggcccaact cgcccacgaa
     1381 atgaaggtca cgaaggaggc cgtccggcag caactcctcg ggcttcaccg gcaaggttgg
     1441 gtggaaaaag aggtccttcg cgaggccaag gtccgcagtg gacggcccac ggtccggtat
     1501 ggcctgaccc ctttggggga ccattttttc cccaaggatt acgacgcttt ggcggctgaa
     1561 ctattggaga ccatcggccg gaggttgggg ccccctgccc tgcgccggct tctttccgat
     1621 atgacagagg ccagggtcag gaaatggcgg cccgtattgg aagggaaacc cctccgcgat
     1681 aaggtcaata gcctcaaaga cctgtatttg gaaaaggatc cctt
//
LOCUS       DASTJJ010000623         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_209791, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000623 DASTJJ010000000
VERSION     DASTJJ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209791"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFR02_07610"
     CDS             <1..344
                     /locus_tag="VFR02_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9870344.1"
                     /translation="SQVREFVSGTGRPSGLVAVYCPHTTAAVSVNENADPDVKRDLLY
                     HLKETIPRSPDFKHNEDNADAHIKAVLTGLSQVFLVEEGKLQLGYWQGVYFLEFDGPR
                     EREVWLKFIPG"
     gene            complement(281..>1014)
                     /locus_tag="VFR02_07615"
     CDS             complement(281..>1014)
                     /locus_tag="VFR02_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9870345.1"
                     /translation="DGMPGMPRKQGLAYWLSRKGYWVFYPRFRGCWESGGRFLRKSPE
                     EDLKDVLDGLFKPFKENTFGKKFRFNPKSIFVVGGSFGGAAAVLSTLDPRVTAAVANC
                     PVVDWAILREEERKETSNPSYTAYLKEAFGEAYRLSPRDWAKLYGGRFYNPAFRAAEI
                     DPAKVLMFHAQDDPYVPWRSVAAFAEKTGARLVLLKKGGHLSTNKTLPAHWRAIAAFF
                     RKAEGHPGMNFSQTSRSRGPSNSKK"
BASE COUNT          182 a          332 c          320 g          180 t
ORIGIN      
        1 cgtcccaggt gcgggaattc gtgtccggaa cgggccggcc ctccgggctg gtggccgtct
       61 attgccccca caccacggcg gcggtctcgg tcaacgagaa cgccgacccc gacgtgaagc
      121 gggacctgct ctaccacctg aaggaaacca tcccccggtc gcccgacttc aagcacaacg
      181 aggacaacgc cgacgcccac atcaaggccg tcctgaccgg gctctcccag gtctttttgg
      241 tggaggaggg caagctgcag ctgggttact ggcagggggt ctactttttg gagttcgacg
      301 ggccccggga acgggaggtc tggctgaagt tcatcccggg atgaccttcg gccttgcgga
      361 agaaggccgc gatggcgcgc caatgggcgg gcaaggtctt gttcgtggat aggtgccctc
      421 cctttttgag caacaccagc cgcgcgccgg tcttttccgc gaaagccgcc accgaccgcc
      481 agggcacata ggggtcgtcc tgggcgtgga acatcagcac cttggcgggg tcgatctcgg
      541 cggcccggaa cgccggatta tagaaacgcc ccccgtagag tttggcccag tccctgggcg
      601 acaggcggta ggcttcccca aaggcctcct tcaaataggc cgtatagctg gggttggagg
      661 tctctttccg ttcctcttcc ctcaggatgg cccaatccac caccgggcag ttggccaccg
      721 cggccgtgac ccgcgggtcc aaggtggaga gcaccgccgc cgccccgccg aaacttcccc
      781 cgaccacgaa gatggacttg gggttgaagc ggaacttttt cccgaaggta ttttctttaa
      841 agggcttgaa gaggccgtcg aggacgtcct tcaggtcctc ttcgggggac tttctcagga
      901 accgcccgcc gctctcccaa cagccccgaa agcggggata gaagacccag taacccttcc
      961 gggaaagcca gtaggccagc ccctgcttgc ggggcatgcc cggcatgccg tcgc
//
LOCUS       DASTJJ010000624         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_697649, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000624 DASTJJ010000000
VERSION     DASTJJ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697649"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFR02_07620"
     CDS             <1..1136
                     /locus_tag="VFR02_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET9870346.1"
                     /translation="AYLSLVTVGGDFLGFQWDNLLLECDLLALFLVPLGISVPRGARV
                     PGLAAFACRWLLFRLMLGSGLVKLESGDPAWRHLTALFYHYQTQPLPTPLAWWMAQLP
                     GAFQRASCFFVLAVELGAPLLLIGPRRFRAYAFPPLALLQVLIALTGNYCFFNLLALG
                     LCFFALEDAAWPARARAWFAPRAGRGVFPPVWARALLLAPLLLISAAQFAGLLGLRWP
                     GRVLAPLEEAAAPFRSVNPYGLFAVMTTRRFEITVEGSDDGAHWKAYGFQDKPGDLSR
                     APRWVAPLQPRLDWQMWFAALGPAEESPWLKAFLTRLLQGSPQVTALLGDDPFPDAPP
                     RSIRAEIAEYRFTDAAERARTGDWWERGEPQLYVPAFSLAGVK"
     gene            1141..>1786
                     /locus_tag="VFR02_07625"
     CDS             1141..>1786
                     /locus_tag="VFR02_07625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870347.1"
                     /translation="MFQEDYWERKSLAKRRDPAHPVVRDYVVPKLDWVRPHLTLGPGA
                     RILDVGAGNGFFSAYLAGWGKVTAVDLSAQMLGMNPAPQKARMAAEHLAFADMAFDLV
                     FCHALLHHVEDLDRVLAEMKRVSRRHVVVMEPNPANPLMALFSALVPEERKALAFGGD
                     FLKAALERQDLRVTASCAFGLLVPNKTPGFLRPLLKVFNFPQPWGMTRILVAERR"
BASE COUNT          214 a          605 c          621 g          346 t
ORIGIN      
        1 cggcctacct ttcgctggtg acggtggggg gcgacttcct gggattccag tgggacaacc
       61 ttttgttgga atgcgacctt cttgcgttgt tcctggtgcc cctggggatt tccgtccccc
      121 ggggcgcgcg ggtgccgggc ctggcggcct tcgcctgccg gtggttgctg ttccggttga
      181 tgttgggctc gggcctggtg aaattggaaa gcggggaccc ggcctggcgc cacctgacgg
      241 cccttttcta ccactaccag acccagccgc tccccacacc cctggcctgg tggatggccc
      301 agctgcccgg ggccttccag agggcgtcct gttttttcgt cctcgccgtg gaactggggg
      361 cgcccttatt gctgatcggt ccgcgccgtt tccgggccta cgccttcccg cccttggcgc
      421 tgttgcaggt cttgatcgcg ctcacgggca attattgctt cttcaacctc ctggccctgg
      481 ggctctgctt tttcgccttg gaggacgcgg cctggcccgc ccgggcccgg gcctggttcg
      541 cgccccgggc cggacggggg gtctttccgc cggtctgggc ccgggccctg ttgctggcgc
      601 ccttgctttt gatctcggcc gcccagttcg ccgggctcct ggggctgcgt tggcccgggc
      661 gggtcctcgc ccccttggag gaggcggccg cgcccttccg gtcggtcaac ccctacggcc
      721 tcttcgcggt catgaccacg cggcgcttcg agatcacggt ggaaggcagc gacgacggcg
      781 cgcattggaa ggcctatggg ttccaggaca agccggggga cctttcccgg gcgccccggt
      841 gggtcgcgcc cctgcagccc cggctcgact ggcagatgtg gttcgcggcg ctgggccccg
      901 ccgaggagag cccctggctg aaggccttct tgacgcgcct gctgcaggga tccccccagg
      961 tcacggcgct gttgggggac gatcccttcc cggacgcgcc gccccggtcc atccgcgccg
     1021 agatcgccga ataccggttc acggacgcgg ccgaaagggc aaggacgggg gattggtggg
     1081 aacgggggga gccccaactt tacgtgccgg ccttcagcct ggccggggta aagtaggccc
     1141 atgttccaag aggattattg ggaaagaaag tcgctggcca agcgccggga ccccgcccat
     1201 ccggtggtgc gggactatgt ggtgcccaag ctcgactggg tgcgccccca tttgaccctg
     1261 gggccgggcg cgcggatcct ggacgtgggc gcggggaacg gttttttctc cgcctatctg
     1321 gccgggtggg ggaaggtcac ggcggtggat ctttccgccc agatgctggg catgaacccg
     1381 gccccccaaa aggcgcgcat ggcggccgaa cacctggcct tcgccgacat ggccttcgac
     1441 ctggtcttct gccacgccct gctgcaccac gtggaggacc tggaccgggt cttggccgag
     1501 atgaagcggg tgtcccgccg ccatgtggtg gtgatggaac ccaacccggc gaaccccctg
     1561 atggcgctct tttccgccct ggtgccggaa gagcgcaagg ccctggcctt tgggggggat
     1621 ttcctgaagg cggcgctgga gcgccaggac ctgagggtca cggcgtcctg cgccttcggg
     1681 ctcttggtgc ccaacaagac cccgggcttc cttcggcccc tgctcaaggt cttcaacttt
     1741 ccccagccct ggggcatgac ccgcatcctg gtggcggagc gccgtc
//
LOCUS       DASTJJ010000625         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1432345, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000625 DASTJJ010000000
VERSION     DASTJJ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432345"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFR02_07630"
     CDS             <1..197
                     /locus_tag="VFR02_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870348.1"
                     /translation="EPAPKGLEALPPEQALRSVQKHLDILPAYLSPRMVLDHLFLELR
                     EGLQKGEVPSFSFTDELSQI"
     gene            306..1742
                     /locus_tag="VFR02_07635"
     CDS             306..1742
                     /locus_tag="VFR02_07635"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET9870349.1"
                     /translation="MNKCAILWKEERPLFLLWALAACAYAALSLFRHWHLESSAYDLG
                     IQDQAVWHYSRFQVPSDSITQFHDLLGDHFSPVMALFAPLYWLCPRVEMVLAAQAFLI
                     TLPVFPIFLFARKRLGRFPAYCLAAAYALSWGVQVFADFDVHELAFAAPAAAFAVYFA
                     DEEKWAPCFISAALLGLCKEDQWLLAAFLGLVLFLRGQRWKGAVLAFAAFSLFVVEVK
                     WLIPFLGQRPYGHWNYSQLGPGPLAALGTCLAHPFRVLGLLFSDQAKSVTLLCLFLPF
                     LFGSFFSPWVLLAVPLIAERMLSDNADFWVMKYQYNLVPMVVLCLAAADGFGRLLGRI
                     QDPVRRGRILRGGAWFLLAVNLVAAQLPHLPLGGLIRRPFWHFTPREALAPQAFALIP
                     PDASVLAQSCLVPHLSHRQVIHDFWWDWETSPPEEDYVVICKGKDAWPLQPEDLDWAV
                     TLKESQGYSPVFQKEGWYVLKKTGRPSP"
     gene            1824..>2429
                     /locus_tag="VFR02_07640"
     CDS             1824..>2429
                     /locus_tag="VFR02_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9870350.1"
                     /translation="MPDKPTFYVTTPIYYVNDVPHIGHAYATIGADALARFKRMDGFD
                     VFFLTGTDEHGQKIAKTAKEKGLEPKQLADQVVERFKEAWVKLGISYDRFIRTTDEDH
                     RKAAQAFFALVQKNGYIYKADYEGWYCLPDEKFLLDSEVKDGKCPDCGRPVEKIKEEN
                     YFFAMSKFQKQIEEHLEKHPDFLQPESRRNELINNFIKPGLA"
BASE COUNT          392 a          819 c          711 g          507 t
ORIGIN      
        1 ccgagcccgc cccgaagggg ctggaggccc tgccgcccga acaagccctg cggtcggtgc
       61 aaaagcacct ggacatcctc cccgcctatc tgtccccccg catggtcctg gaccaccttt
      121 tcctggaatt gcgggaaggc ctgcaaaagg gggaggtacc ctccttttcc ttcacggacg
      181 aattgtccca aatctgatcc ggccgccatt tttcatgtgg tggcggggcc cttcctttgg
      241 gtaacctggg tcccttgccc gtcggcggcg gacaaaatgt tggccggcgg tggatcgggg
      301 tccgaatgaa caagtgcgcc atcttgtgga aggaagaaag accgcttttc ctgttgtggg
      361 ccctcgcggc ctgcgcctac gcggcgcttt ccttgttccg ccattggcac ctggaaagca
      421 gcgcttacga cctgggcatc caggaccagg cggtctggca ctacagccgt ttccaggtcc
      481 cctcggattc gatcacccaa ttccacgacc tgttgggcga tcatttttcc ccggtcatgg
      541 cccttttcgc cccgctttat tggctatgcc cccgggtcga gatggtcctg gcggcccagg
      601 ccttcctcat caccctgcct gtcttcccga tcttcctctt tgcccgtaaa cggttggggc
      661 gtttcccggc ctattgcctg gcggccgcct acgccctgtc ctggggcgtc caggtcttcg
      721 cggatttcga cgtccatgaa ctggccttcg ccgcgcctgc cgccgccttc gcggtctatt
      781 tcgccgacga ggaaaaatgg gcgccttgct tcatctcggc ggccctcttg ggcctatgca
      841 aggaggacca gtggctgctg gcggccttcc tgggcctggt cctcttcctg cgcggtcaac
      901 gatggaaggg ggcggtcttg gcgttcgcgg ctttctcgct cttcgtggtg gaggtcaagt
      961 ggctcatccc tttcctgggg cagcggccct acggccattg gaactattcc caattgggac
     1021 cgggccccct ggcggccctg ggcacctgtc tggcccaccc gttccgcgtc ctggggcttc
     1081 ttttctcgga ccaagccaag tccgtcaccc tcctttgcct tttcctgccg ttcctcttcg
     1141 ggtccttttt ctccccctgg gtgctcctgg ccgtgccctt gatcgccgaa cggatgctgt
     1201 cggacaacgc cgatttctgg gtcatgaaat accagtacaa cctggtcccc atggtggtgc
     1261 tctgcctggc cgccgccgac ggtttcgggc ggttgctggg ccgcatccaa gacccggtcc
     1321 ggcggggccg gatcctgcgt gggggggctt ggttcctctt ggccgtcaac ttggtggcgg
     1381 cccaactgcc ccacctgccc ctgggcgggc tgatccgccg gcccttctgg catttcaccc
     1441 cgagggaagc gctggcgccc caggccttcg ccctcatccc gccggacgcc tcggtgttgg
     1501 cccagtcctg cctggtgccc catctgtcgc accggcaggt catccacgac ttctggtggg
     1561 attgggaaac ctcccccccc gaggaggact atgtggtgat ctgcaagggc aaggatgcct
     1621 ggcccctgca gcccgaggac ctggactggg ccgtgaccct caaggaaagc cagggttatt
     1681 cgcccgtctt ccagaaagag ggatggtacg tgctcaagaa aaccgggcgg ccgtccccct
     1741 gatccgggcc ccgtggggcc cctttttcca ctggttttgg ccccgggccc tgtgtaacct
     1801 gtgctcccga taaggaggcc cggatgcccg acaaacccac tttttacgtc accaccccca
     1861 tctattacgt caacgacgtg ccccacatcg gccatgccta cgccacgatc ggcgccgacg
     1921 ccctggcgcg cttcaaacgc atggacggct ttgacgtctt tttcctgacg ggcaccgacg
     1981 aacacggcca aaagatcgcc aagacggcca aggaaaaggg gctggaaccc aaacaattgg
     2041 ccgaccaggt ggtggagcgc ttcaaggagg cctgggtgaa gctgggcatt tcctacgacc
     2101 ggttcatccg caccaccgac gaggaccacc gcaaggccgc ccaggccttt ttcgcgctgg
     2161 tccaaaagaa cggttacatc tataaggccg actacgaggg ctggtactgc ctgcccgacg
     2221 agaagttcct cctcgattcg gaagtgaagg acggcaagtg cccggattgc gggcggccgg
     2281 tggagaagat caaggaagag aattacttct tcgccatgtc caagttccaa aagcaaattg
     2341 aggaacacct ggaaaagcac ccggacttcc tgcagcccga gagccggcgc aacgaactca
     2401 tcaacaactt catcaagccg ggcctcgcc
//
LOCUS       DASTJJ010000626         1179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1187623, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000626 DASTJJ010000000
VERSION     DASTJJ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187623"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFR02_07645"
     CDS             complement(<1..908)
                     /locus_tag="VFR02_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870351.1"
                     /translation="MADAPTTSPQPAAPPPAGPSRSEAFLNAAKGFGKKLVAFLSTTT
                     VALLGFAVILLTWGAVLADKHFAHLSAVAGPIADHVASWPFLFTGIGLLLVAKSRAMV
                     RDNYAAEAKKTKGEYPHAWKIPLIGTQSLALNLLAFYALGVGIRLWPRLHNVILLLLL
                     FLAFLTYLFWYVTAHFVNRFPKAAGLRIAVMALVLALLAAFAMSQVLLIGLILALFAL
                     LAALVSISVSPFSSPEGRVWIRALCLFGAIGLLVPTAYHYFFGGESQVKLIELNPAVK
                     GLTGEVGALAYSPDGQRLAFSVKKDR"
     gene            complement(944..>1179)
                     /locus_tag="VFR02_07650"
     CDS             complement(944..>1179)
                     /locus_tag="VFR02_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET9870352.1"
                     /translation="ADGRVLTGKRACELGFVDELGNYEDAVDATAKLAGITGTPRVHL
                     DAPSNIGQFLDSLTSLSIFSRTPLGPDLEYKAF"
BASE COUNT          225 a          358 c          432 g          164 t
ORIGIN      
        1 cagcggtcct tcttcactga aaaggccagg cgttggccgt cgggcgagta ggccagggcg
       61 ccgacctccc cggtcaggcc cttcaccgcc gggttgagct cgatcagctt gacctgggac
      121 tcgccgccga agaagtaatg ataggcggtg ggcaccagca ggccgatggc gccgaacagg
      181 caaagggcgc ggatccagac ccggccctcc ggcgaggaaa agggcgagac cgagatggag
      241 accagggccg ccagcagggc gaagagggcg aggatcagcc cgatgagcag cacctggctc
      301 atggcgaagg ccgccagcag ggccaacacc agggccatga cggcgatgcg aagccccgcc
      361 gccttgggga aacggttcac gaaatgggcc gtcacgtacc agaagaggta ggtcaggaag
      421 gccaggaaca acaacaacag caggatgacg ttgtgcaggc gcggccagag gcggatgccc
      481 acccccaggg cgtagaaggc cagcaggttc agggccaggc tctgggtgcc gatgaggggg
      541 atcttccagg cgtgggggta ttcccctttg gtcttcttgg cctcggccgc gtagttgtcc
      601 cgcaccatgg cgcgggactt ggccaccaag agcaggccga tgcccgtgaa gaggaagggc
      661 cagctggcca cgtggtccgc gatggggccg gccacggccg acagatgggc gaaatgcttg
      721 tcggccagca cggcccccca ggtcagcagg atgaccgcga aacccagcag cgccaccgtg
      781 gtggtggaga ggaaggccac cagttttttc ccgaagccct tggcggcgtt caggaaggct
      841 tcggaacggc ttggcccggc cgggggcggg gcggcgggtt ggggcgaggt cgtcggggcg
      901 tcagccaatg gatccctcca agtgcggtaa tgtacccgaa cggctaaaag gctttgtact
      961 ccaggtccgg gcccaacggg gtgcggctga aaatcgagag cgacgtcagg ctgtccagga
     1021 actggccgat gttcgagggg gcgtccaggt ggacccgcgg ggtcccggtg atgccggcca
     1081 gtttggcggt ggcgtccacg gcgtcctcat agttgcccag ttcgtccacg aaccccagct
     1141 cgcaggcccg cttccccgtc aagacccggc cgtcggcgt
//
LOCUS       DASTJJ010000627         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_575488, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000627 DASTJJ010000000
VERSION     DASTJJ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575488"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFR02_07655"
     CDS             complement(<1..848)
                     /locus_tag="VFR02_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870353.1"
                     /translation="MRTRLLSGLLLVWSLAAALPAAAQVNTVTDPIFGRKETYGIGPR
                     AIGMGSAFTALADDASAVYWNPAGLSQMSSYELELSSSPVNFDSRFGFPYYSSIQFVM
                     PIAKENTLAISMFRPFNPQRDYFAGSGLDSADTGEASYILNPSFQESVIMLSYAARFA
                     TLQNFAVGVNVKRITNDPYYIKYFPNTNDTNEAAVAQALKDPVRVVGYGVDIGFLYRI
                     PITKYSEELRFGLNLRDLVSRVEYVNGLQVTTIAQGGVTTVNYNVGPGFETEIPPEIT
                     LGLALT"
     gene            complement(1057..1347)
                     /locus_tag="VFR02_07660"
     CDS             complement(1057..1347)
                     /locus_tag="VFR02_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET9870354.1"
                     /translation="MDRAKRRKISRKAAVALRYDRPAMPAPQVVAKGRGVVAENLIAL
                     ARKNGVPVVEDRLLVETLDQLNVDQEIPPELYQVVAEILVAIYKAETGKTKK"
     gene            complement(1358..>1683)
                     /locus_tag="VFR02_07665"
     CDS             complement(1358..>1683)
                     /locus_tag="VFR02_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870355.1"
                     /translation="TGSARLEVFTEKVGKHPPAKGARAVYLVFTTDFADGDQLQWSAY
                     LKDKNVSLQLYASEDAGERHDLDRMAEDLAKRLRKQGFLLSAPVVRLKKPFRVPEGFR
                     LNVRA"
BASE COUNT          295 a          539 c          511 g          338 t
ORIGIN      
        1 ttggtcaggg ccaaccccaa ggtgatctcg ggcgggatct cggtctcaaa gcccgggccc
       61 acgttgtagt tgaccgtggt gacgccccct tgggcgatcg tggtgacctg caggccgttc
      121 acatactcca cccggctcac caggtcccgc aggttcagcc cgaagcggag ctcttctgag
      181 tacttggtga tggggatgcg gtagaggaat ccgatgtcca cgccgtaacc caccacgcgc
      241 accgggtcct tgagcgcctg tgccacggcg gcctcgttcg tgtcattggt gttggggaaa
      301 tacttgatgt aataggggtc gttggtgatg cgcttcacgt tgacgcccac cgcgaaattc
      361 tgcaaggtgg cgaaacgcgc cgcgtaggag agcatgatca cgctctcttg gaaggagggg
      421 ttcaggatgt agctggcttc cccggtgtcg gccgagtcca ggccgctgcc ggcgaaatag
      481 tcgcgctggg ggttgaaggg ccggaacatc gagatggcca gggtgttctc tttggcgatg
      541 ggcatgacga actgaatgga cgaatagtag gggaacccga accggctgtc gaaattgacg
      601 ggcgagctgg acaattccag ctcataggac gacatctggg aaagtcccgc ggggttccaa
      661 tagaccgccg aagcgtcgtc ggccagggcg gtgaaggccg agcccatgcc gatggcccgg
      721 gggccgatgc cgtaggtctc cttgcggccg aagatggggt cggtgacggt gttgacctgg
      781 gccgcggccg gcagggccgc cgccaggctc cataccaaca gcaggccgct cagcaggcgc
      841 gtgcgcatgt tggatccaag ctgttccgtc gaaaagtatg gttttttcac cggtctcgtc
      901 gtccttttgg ccgggtcctc ccgggccatg ggtttgatgg ggccgtcttc gccacccctt
      961 aacttacacc tttcgggtcc ctctttcagc tttataaatc aaaggatcca ccccaaaccg
     1021 ggcttgcccg gcggccaccc gaccggctcc gccggcctat ttcttggtct tccctgtttc
     1081 cgctttatag atcgcgacca atatctccgc cacgacctgg taaagctcgg gcgggatctc
     1141 ctggtccacg ttcaattggt ccagggtctc caccagcaag cggtcttcca ccaccggcac
     1201 gccgttcttc cgggccaagg cgatgaggtt ctccgccacc accccgcgtc ccttggccac
     1261 cacctggggc gccggcatgg cgggccggtc ataccgcaaa gcgaccgccg ctttccggga
     1321 tatcttgcgc cgtttcgccc ggtccaaaag ccccatgtca ggccctcaca ttcagccgga
     1381 aaccctccgg cacccggaag ggctttttaa gcctcaccac cggggcggac agcaggaacc
     1441 cctgcttcct cagccgtttg gccaagtcct cggccatccg gtccagatca tgccgttccc
     1501 ccgcgtcctc actggcgtac aactgcaagg aaacgttttt atccttgaga taagcgctcc
     1561 attgcagctg gtccccgtcg gcaaaatccg tcgtaaagac caggtagacg gcccgcgccc
     1621 ccttggcggg aggatgcttc cccacctttt cggtgaagac ctccagccgg gccgaaccgg
     1681 tct
//
LOCUS       DASTJJ010000628         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1676793, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000628 DASTJJ010000000
VERSION     DASTJJ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676793"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..>1534)
                     /locus_tag="VFR02_07670"
     CDS             complement(3..>1534)
                     /locus_tag="VFR02_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870356.1"
                     /translation="ITGENELTIGAKRLERPEDDVTLGTRDLSDLNRSPDELPGLEKT
                     RTALSTEDTGASRDRAAILHLGGGNLDTFGGWGLLGQSSPLFQSLLSGGYSSWGGEPA
                     GGGSDGEERYRLGFDGTLFPRGPWTFRFSGGGAALNAVLPYEGSQVETHRAYHLEGGA
                     GGKLSDFSRLDLEVRDEQTDLSTWNGGQTLTSANEFQARGKVEFDEIDPFLRSLSLNG
                     GLRKGSSGGFAPADAKGYRQEWLGASCQWKAGDLDLTTSLQGQEGGEGLDLPLRFYPS
                     ARLLLYLPESAQLEVGYLNSREVDDFQEDDQDRAHLSDAGGFVPPTEHQNEWSLKWTQ
                     KVSETAIFSVFGSDGEIIGYHQWSDQAGPGPPSYLQGLTTLGRVELRQAGANFQFDFD
                     RDTALSVKYQWQQGLDPGDGRGFTDLPEHQVRASLQTGEEVWDAQLGFTWASSRRAFE
                     DRPGDLPAYWTLDLSGDYHLDRAFGLWAKVENPLGETYSLQPGYEEPRFYGRAGVEFS
                     F"
BASE COUNT          249 a          530 c          506 g          249 t
ORIGIN      
        1 cgctaaaaac tgaattccac cccggcccgg ccgtagaacc gcggctcctc atagcccggc
       61 tgcagcgaat aggtctcgcc cagcgggttc tccaccttgg cccatagacc gaaggcccgg
      121 tccaaatggt aatcccccga caagtccagg gtccagtagg ccggcaggtc ccccggccgg
      181 tcctcgaagg cccggcggct ggaggcccag gtgaacccca gttgggcgtc ccaaacctct
      241 tccccggtct gcagggaggc ccggacctgg tgttcgggca ggtcggtgaa gccccgtccg
      301 tcgcccgggt cgagaccctg ctgccactgg tacttgaccg agagggccgt gtcccggtcg
      361 aagtcgaact ggaaattggc cccggcctgc cgcagttcca cccggcccag cgtggtgagc
      421 ccctggagat agctgggcgg accgggaccg gcctggtcgg accactgatg gtaaccgatg
      481 atctcgccgt cggacccgaa gacggagaag atggccgtct ccgagacctt ctgggtccac
      541 ttgaggctcc actcgttctg atgttcggtg ggggggacga aaccgcccgc gtccgagagg
      601 tgggcccggt cctgatcgtc ctcctggaag tcgtccacct cgcgggaatt gagataaccg
      661 acctccagtt gggcgctctc gggcagataa aggagcaggc gggccgaggg atagaaccgc
      721 aagggaaggt ccaacccctc ccccccttcc tggccttgga gcgaggtcgt gaggtccagg
      781 tcgcccgcct tccactggca ggaggccccc agccattcct gccggtagcc tttcgcgtcg
      841 gccggggcga aaccgccgga ggaccccttg cgcagcccgc cgttcaagga cagtgacctc
      901 aggaaggggt cgatctcatc gaattccacc ttgccccggg cctggaactc gttggcggag
      961 gtcaaggttt ggcccccgtt ccaggtcgag aggtccgtct gctcatcccg cacttcgagg
     1021 tcgaggcggc tgaaatccga caatttaccc ccggccccgc cttccaagtg gtaggcccgg
     1081 tgggtctcca cctgcgatcc ctcgtacgga agcacggcgt tcaaagcggc gccgccgccg
     1141 gaaaagcgga aggtccaggg cccccggggg aagagcgtgc cgtcgaaccc caggcggtag
     1201 cgctcctcgc cgtccgatcc cccgcccgcc ggctcgccgc cccaactgga atagccgccc
     1261 gacagcagcg attggaaaag cgggctgctt tggcccagca ggccccagcc cccgaaggtg
     1321 tccaggttgc cgccgcccag gtgcaggatg gcggcccgat cccgggacgc gcccgtgtcc
     1381 tcggtggaaa gggcggtgcg ggtcttctcc aggcccggca attcgtcggg ggaacggttc
     1441 aggtcgctca ggtcccgggt gcccagggtc acgtcgtcct cggggcgttc caggcgtttg
     1501 gcgccgatgg tcagctcatt ttcccccgtg atga
//
LOCUS       DASTJJ010000629         1437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_455027, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000629 DASTJJ010000000
VERSION     DASTJJ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455027"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            572..1264
                     /locus_tag="VFR02_07675"
     CDS             572..1264
                     /locus_tag="VFR02_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9870357.1"
                     /translation="MHKLSVLMTAIALAAFTPLLLRADGTFSGNQLSQAYTPWYAQAA
                     SITGPVTDLAPGAGTLYNLQPGSSLYLVGNSTLHQYQMRAGTLLGSARVAAGDLAAGL
                     KAGSAGPMALVVPLKDFKSRESGLDDNADKALKADQNPQIRFDLKGEKFDGQTLTAQG
                     TLSVAGVAAPVTLSAAAVLKDGTLRLKGVQKLKMTDFKVTPPSISLLVTAITCTDQVE
                     IHYDVLFAAASK"
BASE COUNT          254 a          524 c          406 g          253 t
ORIGIN      
        1 caccaggcag gccagcaaga gttcataggg cacggacagg accgccaccc agggcgccgc
       61 caacaggatg agcatgccgc ccaccaccgc cgcccacctt aaatggatga accacaccaa
      121 cctttccaaa cgcgtctccc cttcgaaacg gaccatggcc tccacccctt tccctttcct
      181 ttcgccaggt ccccatcctc gcgcgggatc ccccgggggc ttatgacccg gctcatattg
      241 gccgggactt tttttcatta acctgtcccc atcccgcgtt caggagcttt tcatgtccca
      301 cgacctttcg ttttccggcg gcgcccccct tttggccttc ttcgccggga gcgtcctttt
      361 cggcgccgcc ttggtggtct cgctgatccc ctgaggcgcc tttctccggt cccggtcaac
      421 gatttcaggg gaaaaccgac catggcgccc acgccatagg gtccccagcc ccggccccgc
      481 ggtcctcgaa aagccctggt ttgcaaggga tccccgcctg gcaccgcgct tgcaatccat
      541 ccgtggaaat cgaatttttg gaggcaaccc catgcataaa ctttcagtcc tgatgacggc
      601 catcgccctg gccgccttca cgccgctgct ccttcgggcg gacggcacct tcagcggcaa
      661 tcaattgtcg caggcctaca cgccctggta cgcccaggcc gcctccatca ccggcccggt
      721 gacggacctg gcgcccggcg ccgggaccct gtacaacctg cagcccggca gttcccttta
      781 cctggtgggc aattccaccc tgcaccaata ccagatgcgg gcggggaccc tcttggggtc
      841 ggcccgcgtg gccgcggggg acttggccgc gggcctgaag gccggttcgg ccggccccat
      901 ggcgctggtg gtgcccctga aggacttcaa gtcccgcgaa tccgggctgg acgacaacgc
      961 cgataaggcc ctgaaggcgg accagaaccc ccagatccgc ttcgacctga agggggaaaa
     1021 gttcgacggc cagaccctca ccgcccaagg caccctctcg gtggccgggg tcgccgcgcc
     1081 cgtcaccctg tccgccgccg ccgtcctgaa ggatgggacc ctgcgcctca agggcgtgca
     1141 gaagctcaag atgaccgact ttaaggtgac gccgccgtcc atttccctgc tggtgacggc
     1201 catcacctgt accgaccagg tcgagatcca ttacgacgtg ctcttcgcgg cggcctccaa
     1261 gtgagcgccc gccgcatatc cacccaaaaa acaaaggagg aaggaacgat gaaaaatctc
     1321 aagaaactgg cgctgggggc ggtgttgctg gcgggcctgc cgggccgcgg ccaatcggcc
     1381 gaaattttcc gcaacgacga cctggacctc tatatcgggg gccgcctgca gaccctg
//
LOCUS       DASTJJ010000630         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1555723, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000630 DASTJJ010000000
VERSION     DASTJJ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555723"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFR02_07680"
     CDS             <1..535
                     /locus_tag="VFR02_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870358.1"
                     /translation="NFPYAYIALAALLQLALSAWFAISMRWITLACPHTQVASRKVWM
                     DSERTVKFCFRCGTRMPWSVEAVEWKDSSWQVYFFQIPPHLFEYALFWVVQSVIFLIL
                     LFLALHALKRAGFQEALAGLALLVVVAGPVGLYYWNRFKRSLVDEKGHVWADEFKRSL
                     VFWGLVLAGLLALWKVW"
     gene            540..986
                     /locus_tag="VFR02_07685"
     CDS             540..986
                     /locus_tag="VFR02_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET9870359.1"
                     /translation="MEKHGQWVLENTGERRRLAFLAGRLGPEELGLELPNGWTVAVVL
                     AHLAFWDLRQASLIRRWVEEGTRPESLDADSVNEPLAQLCAALPPEAALALALDAAEA
                     ADLQVERLTAAQAEALVGMGLERNLRRSLHRRTHLDQIEKALGARP"
     gene            complement(976..>1255)
                     /locus_tag="VFR02_07690"
     CDS             complement(976..>1255)
                     /locus_tag="VFR02_07690"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9870360.1"
                     /translation="PASLYGDIPATQTLELDLIDPERRLSLGGCLLHTFHPGGRSYEQ
                     MPVNAEEAESRWKARFEAVGRVSGDVEAPGPEDNGDFPHTLDLRRLKA"
BASE COUNT          171 a          391 c          442 g          251 t
ORIGIN      
        1 caacttcccc tatgcctata tcgcgctggc ggccctgctg caattggcct tgagcgcctg
       61 gttcgccatc tcgatgcggt ggatcaccct ggcctgcccc cacacccaag tggcgtcccg
      121 gaaggtctgg atggattccg agcggaccgt caagttctgc ttccggtgcg gcacccgcat
      181 gccctggagc gtggaagccg ttgaatggaa ggactcctcc tggcaggtct atttcttcca
      241 gatcccgccc catctgttcg agtacgccct cttctgggtg gtgcagtcgg tcattttcct
      301 catcctgctt ttcctggccc tccacgcgct caaacgggcg ggcttccaag aggccttggc
      361 gggcctggcc ttgctggtgg tggtggcggg ccccgtgggc ctttattact ggaaccggtt
      421 caaacggtcg ctggtcgatg aaaaggggca tgtttgggcg gatgaattca agcgttccct
      481 ggtcttttgg ggtctggtcc tggcgggcct gttggccctt tggaaggttt ggtagggcca
      541 tggaaaagca cgggcagtgg gtcctggaga acacggggga aaggcggagg ctggcctttc
      601 tggccggccg tttgggcccg gaggaattgg ggctggagct gcccaatggg tggacggtgg
      661 ccgtcgtcct ggcccatttg gccttttggg acctgcggca ggcgtccctg atccgccgtt
      721 gggtggagga ggggacccgg ccggaaagcc tggacgccga ctcggtcaac gagcccctgg
      781 cccagctttg cgcggccctg ccgccggaag ccgccttggc cctggccttg gacgcggcgg
      841 aagccgcgga cctgcaggtg gaacggttga ccgcggccca ggcggaggcg ttggtgggga
      901 tggggttgga gcgcaacctg cggcggtccc tccaccgccg gacccacctg gaccagatcg
      961 aaaaggcctt gggcgctagg ccttgagccg gcgcaggtcc agggtgtggg ggaagtcgcc
     1021 gttgtcttcc ggccccgggg cctcgacgtc cccgctcacc cggccgaccg cctcgaacct
     1081 cgccttccac cggctttcgg cctcttcggc gttcacgggc atctgttcat aactgcggcc
     1141 cccggggtgg aaggtgtgca gcaggcagcc gcccagcgac aggcgccttt cgggatcgat
     1201 caggtccagt tccaaggtct gggtggccgg gatgtccccg tagagcgagg ccggc
//
LOCUS       DASTJJ010000631         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_945177, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000631 DASTJJ010000000
VERSION     DASTJJ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945177"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..>1096
                     /gene="alr"
                     /locus_tag="VFR02_07695"
     CDS             203..>1096
                     /gene="alr"
                     /locus_tag="VFR02_07695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9870361.1"
                     /translation="MARAFPAAPAWAEVSLGALAHNLERMKRLAAPAGVIAMVKANAY
                     GHGLVEASRAFLKAGAWGLAVAFPAEGTALRRAGVRAPILVVAPVLKEGALEALRSKL
                     IPQVSSYEGALLVSRAAEKLRLSSVPVQVELDTGMGRVGFRPGEIFRDLGRLLKLPRL
                     RFTAFYAHFATADWADPKFAARQARTFHQVMRGAGFFLSPREKAMPTHLANSAALLTR
                     ALRSPAALARPGIALYGVPPSPRLDAGLKPAMSLRARILHLKTIDKGDAVSYGRTFVA
                     KRPTRVATLGLGYADGLHRALS"
BASE COUNT          155 a          409 c          350 g          182 t
ORIGIN      
        1 caggacgccg acgtggtgct cttcatccac aaggaaaagt ccccggacaa tcccgagcaa
       61 ttcctccacg agctggtatt ggccaaacag cgcaacggcc ccacggagaa catccccgtg
      121 gtcttccgcc gggagttcac caccttcgag tccaagtcca aggtgtccga acgatgatcc
      181 cgggccggcg ggattgaacc ccatggcccg cgcctttccc gccgcccccg cttgggccga
      241 ggtctccctg ggcgcgctcg cccacaacct ggaacggatg aagcgcctgg ccgcccccgc
      301 gggcgtgatc gccatggtca aggccaacgc ctacggccac gggttggtcg aggcctcccg
      361 ggccttcctg aaggcgggcg cctggggttt ggcggtggcc tttcccgccg aggggacggc
      421 cctgcgccgc gcgggggtgc gcgcgcccat cctggtcgtg gcgccggtcc tgaaggaagg
      481 ggccttggag gccctgcgtt ccaagctcat cccccaggtc agttcctacg aaggcgccct
      541 tctggtctcc cgggccgccg aaaaactgcg gctgtcctcc gtccccgtcc aggtcgagct
      601 ggacaccggc atgggccggg tgggcttccg ccccggggag atcttccggg acctgggacg
      661 cctgttgaaa ctgccccgct tgcgcttcac ggccttctac gcccatttcg ccacggcgga
      721 ttgggccgac ccgaagttcg ccgcgcggca ggcccggacc ttccatcagg tgatgcgggg
      781 ggctggtttc ttcctttcgc cccgggaaaa ggccatgccc acccacttgg ccaacagcgc
      841 cgccttgttg acccgcgccc ttcgctcgcc cgcggcctta gcccgcccgg gcatcgccct
      901 ttacggcgtt ccccccagcc cccggctgga cgcggggctc aagcccgcga tgagcctgag
      961 ggcgcgcatc ctccatttga agacgatcga taaaggggac gcggtcagtt atggccgcac
     1021 cttcgtcgcc aagcgcccca cccgggtggc caccctgggg ctgggttacg ccgacggcct
     1081 gcaccgggcg ctttcc
//
LOCUS       DASTJJ010000632         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_211542, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000632 DASTJJ010000000
VERSION     DASTJJ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211542"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="VFR02_07700"
     CDS             complement(<1..>1040)
                     /locus_tag="VFR02_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET9870362.1"
                     /translation="PFLEPCPPALRAQVSVAVDSFQAHLGQKPPGFWLPECAYQPGLE
                     SVLKEFGLGYFFVDTHGLAFAAPRPKYGPFAPAVTPAGVAVFGRDLESSKQVWSSTEG
                     YPGDPDYRDFYRDIGFDLDHAYLYPGEGEGPRRFTGLKYHRVTGPGEAKETYDPERGL
                     ARARAHAADFLAKRRAQMATLRAWMDRPPLVCCLYDAELFGHWWYEGPDFLQALFELS
                     GAGPDAPRWVSPSDYLGLHPRNQVLTPSLSSWGDQGYADFWLNPSNDWIYPPLLRASR
                     RMVEAADRIQRPDALQERALAQAARELMLAQASDWAFMMKTGNHREYARGRVDGHLGN
                     FQALMDELKKGRV"
BASE COUNT          178 a          351 c          360 g          151 t
ORIGIN      
        1 acacgcccct ttttcaactc gtccatcagg gcctggaaat tgcccaaatg gccgtccacc
       61 cggccccggg catattcccg gtggttgccg gtcttcatca tgaaggccca gtcgctggcc
      121 tgggccagca tcagttcccg ggccgcctgg gccagggcgc gctcctgcag ggcgtcggga
      181 cgttggatcc ggtccgccgc ctccaccatg cgccggctgg cccgaagcag ggggggatag
      241 atccagtcgt tggaggggtt gagccagaaa tcggcgtagc cctggtcccc ccaggaggac
      301 agggagggcg tcaggacctg gttgcgggga tgcagcccca ggtagtccga ggggctgacc
      361 caacgggggg cgtccggccc ggcgcccgaa agctcgaaaa gggcctgcag gaaatccggc
      421 ccctcgtacc accaatggcc gaaaagctcg gcgtcataga ggcagcagac caggggcggg
      481 cggtccatcc aggcccgcag ggtcgccatt tgggcgcggc gcttggccag gaaatccgct
      541 gcgtgggccc gcgcccgggc caggccccgt tccgggtcat aggtctcctt ggcctccccc
      601 ggccccgtca cccggtggta tttcaggccg gtgaaacggc gcggcccctc cccctcgccc
      661 ggataaaggt aggcatggtc caggtcgaac ccgatgtcgc ggtaaaaatc ccggtagtcc
      721 gggtcgcccg gataaccctc ggtggaactc cagacctgtt tggaactttc caggtcccgg
      781 ccgaagaccg ccaccccggc gggggtcacc gccggggcga agggcccgta cttgggccgg
      841 ggcgcggcga aggccaggcc gtgggtgtcc acgaagaaat agccgagccc gaattccttc
      901 agcaccgact ccaacccggg ctggtaggcg cattcgggca gccagaagcc gggcggtttt
      961 tggcccaaat gggcctggaa ggaatccacc gccaccgaca cctgggcccg cagggccggc
     1021 gggcagggtt ccaaaaaagg
//
LOCUS       DASTJJ010000633         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_211577, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000633 DASTJJ010000000
VERSION     DASTJJ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211577"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFR02_07705"
     CDS             complement(<1..260)
                     /locus_tag="VFR02_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870363.1"
                     /translation="MIPPELQKHLELCRRLGIRYLSPMGEAPSQAPETRPSPAQAAPS
                     AVAATPPEGGAPPWLTAPGDTARALKLTAFDREICACQKCPLG"
     gene            complement(274..1227)
                     /locus_tag="VFR02_07710"
     CDS             complement(274..1227)
                     /locus_tag="VFR02_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870364.1"
                     /translation="MRSGSFPARTGLALALLTAPAAAFAADNGGLNPAAYWDLCPGGS
                     PSAMGGAAEGMRGDLAGAFYNPAGLAGLEGTEVQVGHAALSLGRSLNYLAVGASYQEK
                     YHFGLALLAYSDGSDLQARSGPSVAPDAVFSDLALSVLASLAFPLGPDWDLGVSARVL
                     TQAFGLADLPTGFGLGEDLALQYHPTADQCWGLTLSNALADFLYSDGTDAPLPADLRL
                     GLSQAFPDLDLKGALDLDWRTDLGPAPHVGLEWRPVESLAFQGGYWVQLDGGEDGIGF
                     GASLSLGLSGGVRTDLGYSVSTDRLLPGSLTQQLNLRAHFL"
     gene            complement(1224..>1750)
                     /locus_tag="VFR02_07715"
     CDS             complement(1224..>1750)
                     /locus_tag="VFR02_07715"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9870365.1"
                     /translation="TPLVAGAAALLLALNPSLTNLEVADRLINSAKSLNGDRGWDPAT
                     GYGLLDLARALQADGAQLTPYLNTFNSPNPFSLQGDGSTNITLAIDRAEPVELTIRDA
                     GGNLVLHRTYRASELNDNPANPQFKSYYVAWDGRNGAGNQVAPGVYFYAVTAGGVTGR
                     NKIVVIKGLFTGSR"
BASE COUNT          292 a          590 c          627 g          241 t
ORIGIN      
        1 cccaaggggc atttttggca ggcgcatatc tcccggtcaa aggcggtcag tttcaaggcg
       61 cgcgcggtat cccccggggc ggtcagccaa gggggcgccc caccctcggg cggagttgcc
      121 gcaacggcgg atggggccgc ctgggcgggg gagggtcgcg tctccggggc ctgggacggg
      181 gcttccccca tcggggaaag gtagcggatg cccagccggc ggcacagttc gaggtgtttt
      241 tgaagttcgg gggggatcat ggatgccgcc gttctaaagg aaatgggccc gcagattcaa
      301 ctgctgggtc agggaacccg gcagcaggcg gtcggtggag accgaatagc ccaggtcggt
      361 cctcacccca ccggacaatc ccagggaaag cgaggcgccg aaaccgatcc cgtcctcccc
      421 cccgtccagt tggacccagt agcccccctg aaaggcgagg gactccaccg ggcgccactc
      481 caatcccacg tggggcgccg gacccaggtc ggtgcgccaa tccaggtcca acgccccctt
      541 caggtccagg tccgggaaag cctgggaaag ccccaaccgg aggtccgccg gcaggggggc
      601 gtcggtcccg tccgaataaa ggaagtccgc caaggcgttg gagagggtga ggccccagca
      661 ctggtccgcg gtgggatgat actgcagggc caggtcctcg cccaagccga aaccggtggg
      721 caggtccgcc aggccgaagg cctgcgtgag gacccgggcg ctcaccccca ggtcccaatc
      781 cggtcccagg gggaaggcca gcgaggccag cacggacagg gccaggtccg agaagaccgc
      841 gtccggcgcc acggaaggcc cggaccgggc ctgcaggtcc gaaccgtcgc tgtaggccag
      901 cagcgcgagc ccgaaatggt atttttcctg gtagctggcc ccgacggcca agtagttcaa
      961 gctgcgcccc aacgagagcg ccgcgtgccc cacctggact tccgtgccct ccagccccgc
     1021 caaacccgcc gggttgtaaa aggcccccgc caggtcgccg cgcatgcctt ccgccgcgcc
     1081 gcccatggcg gaaggcgagc cgcccgggca caggtcccaa taggccgccg ggttgagccc
     1141 cccattgtcc gccgcgaagg cggccgccgg agcggtcaaa agcgccaggg cgaggcccgt
     1201 gcgggccggg aaggatccgg acctcatcgg gaccccgtga agaggccctt gatcaccacg
     1261 atcttgttgc ggcccgtcac gcccccggcg gtcacggcgt agaaataaac gcccggcgcc
     1321 acctggttgc ccgcgccgtt gcgcccgtcc caggcgacat agtaggactt gaactggggg
     1381 ttggcgggat tgtcgttgag ctcggaggcc cgataggtcc ggtgcagcac caggtttccc
     1441 cccgcgtccc ggatggtgag ctccaccggc tcggcccggt cgatggccag ggtgatgttg
     1501 gtgctgccgt ccccctgcaa cgagaagggg ttggggctgt tgaaggtgtt gagatagggg
     1561 gtgagttggg cgccgtcggc ctgcagggcg cgggccaggt ccaaaagccc gtaccccgtc
     1621 gccgggtccc agccccggtc gccattgagg ctcttggcgc tgttgatgag gcggtccgcc
     1681 acctccaggt tggtcaggga ggggttcagc gccaaaagca gggccgccgc cccggccacc
     1741 aggggcgtgg
//
LOCUS       DASTJJ010000634         7205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_699298, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000634 DASTJJ010000000
VERSION     DASTJJ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699298"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFR02_07720"
     CDS             complement(<1..480)
                     /locus_tag="VFR02_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET9870366.1"
                     /translation="MTNPSKSWKTVLQDQMPPDWAKELDEFEANIQLNKQGKIEPRIF
                     AETRLRRGAYGQRYDNGHRNDGKQVRKLPFPTQGVWKGPDTYWDAPGMLRIKFPWGGI
                     NASQMDVMADLSEEYSDGISHITTRQAIQLHFIHIEDAPDIMRRLAAVGITTREACGN
                     "
     gene            complement(589..1119)
                     /locus_tag="VFR02_07725"
     CDS             complement(589..1119)
                     /locus_tag="VFR02_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9870367.1"
                     /translation="MRGADLLRQELAAMKGRVQGWSRAKKLAAFAGGTGRVTRTLSKF
                     PRSMWGYTRRQGNWTITQVLWHLADQEAHLFLRLRKALAEPGGMVSAYDQEKWDRGLL
                     YSKSDPLQAKDLLLSLRRANADLLKKTGARAWKGRVRHPEWGVLTLEYLVGLNIWHLE
                     HHLAQMGRRFQEWKAR"
     gene            complement(1448..1948)
                     /locus_tag="VFR02_07730"
     CDS             complement(1448..1948)
                     /locus_tag="VFR02_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET9870368.1"
                     /translation="MHSGFFLLLACILACEAVGGLSGLFTQFTRNRWFVGLRKSALQP
                     SGKVLELGWAVCYGLMGAAFYLVLKAPSGHYGHTASFIIFGFLLAVGLLWPLSFFKMK
                     HPFLAFVELLIFWMFALGTIIWFSYAAPAAFFLLLPVMGWILFEAFMSFMLMMLNEGP
                     TAPSHM"
     gene            complement(2134..2499)
                     /locus_tag="VFR02_07735"
     CDS             complement(2134..2499)
                     /locus_tag="VFR02_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9870369.1"
                     /translation="MSMTKEDVREKMKEKDVVLLNVGAEENYLKLHIQGSLNQPLTFD
                     RGFFAQEVERKFGRNKFFITYGPETAGIENMNGSQILHEKGFQTEHFPGGLAEWEKAG
                     WPTEGSASKPKPASIGVSK"
     gene            complement(2646..3542)
                     /locus_tag="VFR02_07740"
     CDS             complement(2646..3542)
                     /locus_tag="VFR02_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET9870370.1"
                     /translation="MGKTYAVMGATGHIGRVVAEQLLAKGHAVRALGRDEKKLSALKA
                     KGAQAVTGAFDDAAALTALFNGVDGAFLMTPPSYDAESLSDAQDRVGEAVVKALSATG
                     LKYAVNLSSVGAELPSGSGPITGLYRQEQRLAKLGGLNLVNLRPTAFMENSFFAIPVI
                     KAYGVFGAAAPADYPLPTVATRDIAAKVVEFLEALSFQGQRFFDLTGPREYTQTELVK
                     VLGAAIGKPDLKYQQTPLDQIKAGMLGMGMRPKTVDLMIEMYGAGNAGKLHPNQELTA
                     EHRGSTTLEQFAPAFAAAYQQA"
     gene            complement(3653..3994)
                     /locus_tag="VFR02_07745"
     CDS             complement(3653..3994)
                     /locus_tag="VFR02_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9870371.1"
                     /translation="MKKALMALGAAVLGAAVLAGCATTGVPAPSADLVARAQARWPGT
                     TARDLAAGRRLYAANCAACHELHDASDLSPVGWEKIMAKMQKKAHIDDLTKESILKYL
                     ESATERTDGRD"
     gene            complement(4005..4787)
                     /locus_tag="VFR02_07750"
     CDS             complement(4005..4787)
                     /locus_tag="VFR02_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870372.1"
                     /translation="MRTSLRKWWMAAALAALPALSWAGDRQFLYNYQSGVLGAGEREV
                     ETYSTYRFGRDRFYSALDQSLEFETGLGGGVQTSVYLNFTQELGRDPADGSGVVAQGP
                     TLDGVSNEWKFKLADASADALGLGLYVEPEFEPDELDLELKAILDKKIGPFLAAFNLL
                     AAPAFNYADGSSSFLLRPSVGLGAFVTGRLFLGLEAMDENFYDDAPLRSVFSMGPLVQ
                     YSGDNWWVGLTFLPQLASFGSPTLDFTDSQRDQVRLATSFSL"
     gene            complement(4850..5026)
                     /locus_tag="VFR02_07755"
     CDS             complement(4850..5026)
                     /locus_tag="VFR02_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HET9870373.1"
                     /translation="MAPDDAQRRPAPAGPPPQPRESRSEDLLGPGGRELLIRHGAQTY
                     RLRLTQNGKLILTK"
     gene            complement(5179..5580)
                     /locus_tag="VFR02_07760"
     CDS             complement(5179..5580)
                     /locus_tag="VFR02_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870374.1"
                     /translation="MKNYFKAAVALTLFAGLGLNAGCSSNKRIEGADAPSMAPTPTAM
                     GSYTVHRGDSLWAISGKSKIYGDPYAWPLLFKANRDTIQDPDILEVSEVLKVQKGVSA
                     DELSHARSLASKTPKYVPHAGPRATLPLNYF"
     gene            complement(5618..5833)
                     /locus_tag="VFR02_07765"
     CDS             complement(5618..5833)
                     /locus_tag="VFR02_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870375.1"
                     /translation="MKKEHFIGIFILYALLFAAGHWPVWREVAAALREGTGHGHPPVW
                     AEGWTPGPGVWVQKGSLAQEEVEKTRS"
     gene            complement(5910..6239)
                     /locus_tag="VFR02_07770"
     CDS             complement(5910..6239)
                     /locus_tag="VFR02_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870376.1"
                     /translation="MTRFSIHRSLVFLLLVAAAVVLKPLMARAGLELVAQSSLTVKAS
                     PITWGTPARAAGAHYRVDKMERDSVTGAFLKTETRPARADLAFAPSTVPPGRPLPLRT
                     APRVLRL"
     gene            6766..>7205
                     /locus_tag="VFR02_07775"
     CDS             6766..>7205
                     /locus_tag="VFR02_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9870377.1"
                     /translation="MSQRRVVVTGLGTVNPLGPGVEPTWKAVLEGKSGVGPITLFDAK
                     DCPVKIAAEVKGFDPTVPLPSPITGVAGRDPLTQAVHPKDVKRFGRFTHLGVAAGLQA
                     YLDSGLDRVRGGLEDDRLGVNVGVGQGGLPEIQAVHDDYLAKGFR"
BASE COUNT         1317 a         2286 c         2299 g         1303 t
ORIGIN      
        1 gttgccgcag gcctcgcggg tggtgatgcc cacggccgcc aggcggcgca tgatgtcggg
       61 ggcgtcctcg atgtggatga agtgcagctg gatggcctgg cgggtggtga tgtggctgat
      121 gccgtccgag tattcctccg acaggtcggc catgacgtcc atctgggagg cgttgatgcc
      181 gccccagggg aacttgatgc gcagcatgcc gggcgcgtcc caataggtgt cggggccctt
      241 ccacacgccc tgggtgggaa aaggcagctt gcgcacctgc ttgccgtcgt tgcggtggcc
      301 gttgtcgtag cgttggccgt aggcgccgcg gcgcagccgc gtctcggcga agatgcgggg
      361 ctcgatcttg ccctgcttgt tgagctggat attggcttcg aactcgtcca gttccttggc
      421 ccaatccggg ggcatctggt cctgcaggac ggttttccag ctcttcgacg ggttcgtcat
      481 ggccaaaacc tctttttctt attgagaaat gggacaggcg ccacggaagg gcgctgagcg
      541 aagggctaca gtaatggaag ggaatttaaa aatcaataaa gcaatggctc agcgggcctt
      601 ccattcttgg aatctccgcc ccatttgggc caggtggtgc tccaagtgcc agatattgag
      661 gcccaccagg tactcgaggg tcaacacacc ccattcggga tgccggaccc ggcctttcca
      721 ggcccttgcc cccgtttttt taagaaggtc ggcgttggcg cgccgcagcg acaagagcag
      781 gtccttcgcc tggagggggt cggacttgga ataaaggagg ccccgatccc atttttcctg
      841 gtcataggcc gagaccatgc cgccgggttc ggccagcgcc ttgcgaaggc gcaggaagag
      901 gtgggcttcc tggtccgcca ggtgccagag cacttgggtg atggtccagt tgccctggcg
      961 gcgggtgtag ccccacatgg agcgggggaa cttggagagg gtccgggtga cccggccggt
     1021 gccgcccgcg aaggccgcga gctttttggc ccggctccag ccctggaccc ttcccttcat
     1081 cgccgccaat tcctgccgca agagatccgc cccccgcatg ggcccggatg ccatggtcca
     1141 cctcccgaag aagatgggcc attatatccg gaaggcccgg atcaagggcg gaacctcttc
     1201 accgcaggcg cccaagggtg tccccgtccc aaaaaattca atgctgggcg acacgaaggc
     1261 gcgaggaaat aaaggggaag gacgaaaaaa ccattcgaaa acctcaaaac ccaaaacttt
     1321 caccacagag gcgcagagac gcggggaaaa catttttatc tcttatgcct tttgttttcc
     1381 aaccatcgcc ccaactttgc gtctccgcgt ctctgcggta aaagagcttt tgaggcggat
     1441 cttaaattca catatggctg ggcgccgtcg ggccctcgtt gagcatcatc aacatgaagc
     1501 tcatgaaggc ctcgaacagg atccaaccca tgacgggcaa aagcaggaaa aaggccgccg
     1561 gggccgcgta ggagaaccag atgatcgtcc ccaaggcgaa catccaaaag atcaaaagtt
     1621 ccacaaaggc caggaaggga tgtttcatct tgaaaaagga aagcggccaa agcaggccca
     1681 ccgccagcag gaaaccgaaa atgatgaagg aagccgtatg gccgtaatgg cccgaagggg
     1741 ccttcaagac caggtagaaa gcggccccca tcaggccgta acagacggcc caacccaatt
     1801 ccaggacctt gccggaaggc tgcagggcgg acttacgcaa tcccacgaac cagcggtttc
     1861 gggtgaactg ggtgaagagg ccggaaagac cgccgaccgc ctcacaggcg aggatacagg
     1921 caaggagaag gaaaaaaccg gaatgcatgg ggacctcctg aaaggagaac aagcaccgga
     1981 agccgtcgaa gtcccaaggg ctgcggagga cccgccgtcc cactctcggc ttcacttcca
     2041 gtgtggaccc ggggcccggc ccttttcaag gcttttcggg acctggaaag taatggtttt
     2101 cccccgggga tccccggggg caaaagcggg aagctacttg gaaacgccga tggacgcggg
     2161 tttgggtttg gacgcggagc cttccgtggg ccacccggcc ttttcccact ccgccaaacc
     2221 cccgggaaaa tgctcggttt gaaaaccctt ttcatgaagg atctgggagc cgttcatgtt
     2281 ctcgatccct gcggtctccg gcccataggt gatgaagaac ttatttctcc cgaattttcg
     2341 ttccacttcc tgggcgaaaa aaccgcggtc gaaggtcagc ggctggttca gggatccctg
     2401 gatgtggagt ttgaggtaat tttcttcggc gccgacgttc aaaagcacca cgtccttttc
     2461 cttcatcttt tcccgtacat cttccttggt catcgacatg gggccctctt tctatccttg
     2521 gtgttggttc caccctaggc cgaaccgaag gggatagata gtcggggatg aaataaagcc
     2581 ccgcaaaggg cctttgcggg gctttatcgc cgtgcccgaa agcccggccc cttagatccc
     2641 gccgatcagg cttgctggta cgccgccgcg aaggccgggg cgaactgctc cagcgtcgtg
     2701 cttccccggt gctcggccgt gagttcctgg ttggggtgca gcttgcccgc gttgcccgcc
     2761 ccgtacatct cgatcatcag gtcgaccgtc ttaggtctca tgcccatccc cagcatgccg
     2821 gccttgatct ggtccaacgg cgtctgctgg tacttgaggt cgggcttgcc gatggccgcg
     2881 cccagcacct tcaccagctc ggtctgggtg tattcccggg ggcccgtcag gtcgaagaac
     2941 cgctggccct ggaaggaaag ggcttccagg aattccacca ccttggccgc gatgtcccgg
     3001 gtcgccaccg tggggagcgg gtagtcggcg ggcgccgcgg ccccgaagac cccgtaggcc
     3061 ttgatgaccg ggatggcgaa gaagctgttc tccatgaagg ccgtggggcg caggttcacc
     3121 aggttcagcc ccccgagctt ggcgaggcgc tgttcctggc ggtaaagccc ggtgatgggg
     3181 ccggagcccg acggcagctc ggcgcccacc gagctgaggt tgacggcgta tttcaggccc
     3241 gtggccgaca gggccttcac cacggcctcg cccacccggt cctgggcgtc cgagagcgac
     3301 tcggcgtcgt aggaaggcgg cgtcatgagg aaggcgccgt ccacgccgtt gaaaagggcg
     3361 gtcagcgccg ccgcgtcgtc aaaggcgccc gtcacggctt gggccccctt ggccttcagg
     3421 gccgacaatt ttttctcgtc ccggcccagg gcacggaccg cgtgtccctt cgccagcaac
     3481 tgctccgcca ccacccggcc gatatggccc gtggctccca tcacggcgta ggtcttcccc
     3541 atggtcgcct cctaaaatga agatccaccc catctttaag ggaggagggg tggatttcaa
     3601 gccgcgccgg gcggcgccgc gtcaatttca ggtaactttc gggaaaccgg cctcagtcgc
     3661 ggccgtcggt cctttcggtg gccgactcca ggtacttcaa gatcgactcc ttggtcaggt
     3721 cgtcgatatg ggccttcttc tgcatcttcg ccatgatctt ttcccaaccc acgggcgaaa
     3781 ggtccgaggc gtcgtgcagc tcatggcagg cggcgcagtt ggccgcgtaa agccggcgtc
     3841 ccgccgccaa gtcccgggcc gtggtgccgg gccagcgggc ctgggcccgg gccaccaggt
     3901 cggccgaggg cgcgggcacc cccgtggtgg cgcagcccgc caggaccgcc gcgcccagca
     3961 ccgcggcccc caaagccatc aacgcttttt tcatggatcc cgccttacaa actgaaggac
     4021 gtggccaagc gcacttgatc acgctgactg tcggtgaagt cgagcgtggg gctgccgaag
     4081 ctggccaact ggggcaggaa ggtcaggccc acccaccagt tgtcgcccga atattggacc
     4141 agcgggccca tggaaaagac cgagcgcagg ggcgcgtcgt cgtaaaagtt ctcgtccatg
     4201 gcctccagtc ccaggaaaag ccgccccgtg acgaaagctc ccaggcccac cgaggggcgc
     4261 aacaggaagg aactggaccc gtcggcgtag ttgaaggccg gggcggccaa caggttgaag
     4321 gcggccagga aaggcccgat cttcttgtcc aggatggcct tcaattccag atccagctca
     4381 tccggctcga actccggctc cacgtaaagg cccagaccca gggcgtccgc ggaagcgtcg
     4441 gcaagcttga acttccactc gttggaaacc ccgtccaacg tgggcccctg ggccaccacg
     4501 ccgctcccgt cggcggggtc ccggcccagt tcctgggtga aattcaggta gaccgaggtc
     4561 tggaccccgc cgcccagtcc cgtctcgaat tccaggcttt ggtccagggc cgaatagaac
     4621 cggtcccggc cgaagcggta ggtgctgtag gtctcgacct cccgctcccc cgcgcccagg
     4681 acgcccgact ggtaattgta aaggaactgc cggtccccgg cccacgacag cgcgggcagg
     4741 gcggccaacg ccgcggccat ccaccacttc ctcaagctcg ttctcatgat gtcttctccc
     4801 gttcggaatt tttcagcggc ctggctttcc cggtcgggat ggctggggtt cacttggtca
     4861 agatcagttt gccgttctgg gtcaggcgaa ggcggtaggt ctgcgccccg tgccggatga
     4921 gcaactcccg tccccccggc ccgagcaggt cctcactgcg cgactcccgc ggctggggcg
     4981 gcggccccgc gggggcgggt cggcgctggg cgtcgtccgg tgccatcctc gggtcctttc
     5041 ccacggctta tctgatattg aaaatcatta tcaattatct aaaagacggc gttcctgtca
     5101 accggaatct tcgggggaaa ggaacaaaaa aaaggccgtc cccccagggg ggacggcctt
     5161 cgaaagggtc cggctaggtc agaagtagtt gagcggcaag gtggcccggg gccccgcgtg
     5221 cggcacgtac ttcggggtct tggaggccag cgagcgggcg tggctcaatt cgtcggccga
     5281 aacgcccttc tgcaccttca acacctcgct cacttccagg atgtccggat cctggatggt
     5341 gtcccggttg gccttgaaaa gcaggggcca ggcgtagggg tcgccataga tcttggactt
     5401 ccccgagatg gcccagaggc tgtcgccccg gtggaccgta tagctcccca tggccgtcgg
     5461 cgtgggggcc atgctggggg cgtcggcccc ctcgatgcgc ttgttgctgg agcatcccgc
     5521 gttcaggccc aacccggcga agagggtcaa ggccaccgct gctttgaagt agtttttcat
     5581 cgttagtccc tttctggtat tgatctaagt gtacagctta actcctggtc ttttccactt
     5641 cttcttgggc caaagagccc ttttgcaccc acacgccggg tcccggcgtc catccctcgg
     5701 cccagaccgg cggatgaccg tgcccggtcc cctcccgaag tgcggccgcc acctcccgcc
     5761 acacgggcca atggcccgcg gcgaaaagca gggcatagag gatgaatatt cctatgaagt
     5821 gttctttttt catggcgcct cctttcaccg gctccgccct catcaggcgg gcttggccgc
     5881 ggttcgcgcg gccgctcgga aaggacggct cagaggcgaa ggaccctggg ggcggtcctc
     5941 aatggcagtg gcctgcccgg cgggaccgtc gacggggcga aggccaggtc ggcccgcgcg
     6001 ggccgggtct cggtcttcag gaaggccccg gtgaccgaat ccctttccat cttgtccacc
     6061 cggtaatggg ccccggcggc gcgcgcgggg gtcccccagg tgatgggcga cgccttcacc
     6121 gtgagggagg actgggcgac cagctccagg ccggcgcgcg ccatcagcgg cttcaagacc
     6181 accgcggcgg ccaccagcaa caggaaaacc aggcttcgat ggatggaaaa acgtgtcatg
     6241 ggcctattgt aatcacccgg ggtcgggatg caagccccgg cttcgagaac gggggctgat
     6301 ctccaccgtc atcggcacgg aacggacggc cggggtcccg gaggccaccc gatgggcgtt
     6361 tttgggccaa taacccgcca caaacagggc caagtaggcc aggccccaaa gaggggcgtc
     6421 ggacgttggg aaaacctttc cattcgcatg gggatcggac gccccattcg gccgggaagt
     6481 ttcaatccgg cccggtttcc cgggccaggg acgcctccac ttcgcgggcg atctcacgga
     6541 ccctttcgtc cagcaagtcg tccgaaagga tcgggtcggg ttccatggcg ggcctttccg
     6601 ggaagggctt cttccctgga tatccagtgg cccggcggga ctttttattc cccgggtccc
     6661 gctcgaaata agggaaaaaa ggggcgcggc cggccccggc tttccttttc atcgggcgtt
     6721 tcgcataatg accgccgttc aaaaaaccaa agcgaggcgc gcgacatgag ccaacgacgg
     6781 gtggtggtga ccggcctggg gaccgtcaat ccccttgggc cgggggtgga accgacctgg
     6841 aaggcggtcc tggagggaaa aagcggcgtc ggccccatca ccctcttcga cgccaaggac
     6901 tgtcccgtca agatcgccgc cgaggtcaag ggcttcgacc ccaccgtccc cctgccgtcc
     6961 cccatcacgg gcgtggcggg ccgggacccc ctcacccagg ccgtgcatcc caaggacgtc
     7021 aaacgcttcg gccgtttcac ccacctgggc gtggcggccg gcctgcaggc ctatttggac
     7081 tcgggcctgg accgggtccg gggcggcctg gaggacgacc ggctgggggt caacgtgggc
     7141 gtgggccagg gcggcctgcc cgagatccag gccgtgcacg acgactacct ggccaagggc
     7201 ttccg
//
LOCUS       DASTJJ010000635         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_333603, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000635 DASTJJ010000000
VERSION     DASTJJ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333603"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1095)
                     /locus_tag="VFR02_07780"
     CDS             complement(85..1095)
                     /locus_tag="VFR02_07780"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9870378.1"
                     /translation="MPTLKTSSWVRWGTLTLAFVGAVSWYLSGDLVAAGASAAAALVI
                     LFFSWRALWFQVHVLSLRELEIFLVRSRILPPDHKFASAKSLSQIQKTRRELMDMGYE
                     EFKTHQKKLQEARHVLDKFVGTKASQFATQTGNKAVWEGQLTKVIVLFSDVRGFTSMT
                     EKLKPQETVRYLNRMFSELEELVSMAGGEVNKYIGDAVLAFFPFPEENPAPATKRVLQ
                     AALRMQDSFHEIQGKFRETYSEVVNTGLGVGIAAGDVILGNLGSSRRMEFTLIGDTVN
                     LASRLCSIAEDGQVLINTELAHVAAEQFRMEALDPVRIKGKSGTYTPYWVQGERIQQG
                     LA"
BASE COUNT          220 a          428 c          393 g          234 t
ORIGIN      
        1 aaggtctttc gcatggtccc ctcctgcttt ccgaagaata cccggaagaa ggggcgggag
       61 ttacaagccc ccggccggcg ggacctaggc caatccctgc tggatgcgct cgccctggac
      121 ccagtagggg gtgtaggtgc cggacttgcc cttgatgcgc accggatcca gggcctccat
      181 gcggaactgc tcggcggcca cgtgggccag ttcggtgttg atgagcacct ggccgtcctc
      241 ggcgatggaa cagagccggg aggccaggtt gaccgtgtcg ccgatgaggg tgaattccat
      301 gcgccgggac gaacccaggt tgcccaggat cacgtccccg gccgcgatgc ccacgcccag
      361 gcccgtgttc accacctcgc tgtaggtctc ccggaacttc ccctggatct catggaaact
      421 gtcctgcatg cgcagcgccg cctgcagcac ccgcttggtc gccggggcgg ggttttcctc
      481 agggaagggg aaaaaggcca gcacggcgtc cccgatgtac ttgttcactt ccccgcccgc
      541 catcgacacc agttcctcca gttcggagaa catgcggttc aggtagcgga ccgtctcctg
      601 gggcttgagc ttctccgtca tggacgtgaa gccgcgcacg tccgagaaca gcacgatcac
      661 cttggtcagt tgcccttccc aaaccgcctt gttgcccgtt tgggtggcga actgggaggc
      721 cttggtgccc acgaacttgt ccaacacgtg ccgggcttcc tggagctttt tttggtgggt
      781 cttgaactcc tcgtagccca tgtccatcag ttcccgccgg gtcttctgga tctgggaaag
      841 cgacttggcc gacgcgaact tgtggtcggg aggcaggatt cggctgcgca ccaggaagat
      901 ctccaattcc cgcaaggaca acacgtgcac ctggaaccaa agcgcccgcc aggagaagaa
      961 caggatcacc agcgcggccg ccgccgacgc gcccgcggcc accaggtcgc cggacaggta
     1021 ccaggagacg gcccccacga aggctagcgt cagggtgccc caccgcaccc aggacgaggt
     1081 cttgagggtc ggcatgagcc tttcttcgaa ttcctccgcc atgggtctat ttcaccccgc
     1141 tctggtccaa cgccccggcc acccgccggt agaggtcctc ggacgactcg tccacgttca
     1201 agtgcgaatg gtccagcacg atggagtcgg ccgtcttgcg caaccccccc acgggccggt
     1261 tgtagtcccg ctcgt
//
LOCUS       DASTJJ010000636         1419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_699565, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000636 DASTJJ010000000
VERSION     DASTJJ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699565"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VFR02_07785"
     CDS             <1..1367
                     /locus_tag="VFR02_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870379.1"
                     /translation="LWAKSLTASVLAQALGGVACQKLTDTALGLETLNYLPEVEEGGQ
                     SRRRPLEFFSSKEGQDAVKTYSYAYLPAPEKAAMQKFLEDGKLNAPMDVRENLKFFED
                     RLFAGLRECKTWDAYHLYTLKILDELSGTQRLTFLGSQFWASEPFQEFTAHWLLNLPA
                     MTDAYNQSLNEYRAKHQIKHELTPLPNLKFEDWWFELPFWGLNKYHQRHSLWAKVDGD
                     RMILRMKGGDSNYSISTKDFNRELKALPISFWPKALPQTLFCRLYLCDYFIHGVGGGA
                     YERVNDLLWEKVFKSKPVDFGVVTATWKVDPVQSDKIAEIVRHEERILFWERALSKNP
                     ETLFTRREAWEKELPEFIRKEFAPLADDPELAKLAQEKTRCVESLKDPAKKPAAAKRI
                     GELNQALAGRYDPLVKALEKGLMDIEKLKKVQEALDFREYPFFAHSPKIFPEMKKRLE
                     ESLA"
BASE COUNT          287 a          461 c          433 g          238 t
ORIGIN      
        1 gcctttgggc caagagcctg acggccagcg tgttggccca ggccctgggc ggcgtggcct
       61 gccaaaaact caccgacacc gcgctgggcc tggagacgct gaactacctg cccgaggtgg
      121 aggagggcgg ccagtcccgc cgacgccccc tggaattctt ctcctccaag gagggccagg
      181 acgcggtcaa gacctattcc tatgcctacc tgccggcgcc cgagaaggcg gccatgcaga
      241 agttcctgga ggacgggaaa ctcaacgccc cgatggacgt ccgggagaac ctgaagttct
      301 tcgaggacag gcttttcgcg ggtctgcggg aatgcaagac ctgggacgcc taccacctct
      361 acacgctcaa gatcctggac gagctctcgg gcacccagcg gctcaccttc ctgggcagcc
      421 agttctgggc ttccgaaccc ttccaggaat tcaccgccca ctggctgctg aacctgccgg
      481 ccatgaccga cgcctacaac cagtccctca acgagtaccg cgccaagcac cagatcaagc
      541 acgagctcac ccccctgccc aacctgaagt tcgaggactg gtggttcgag ctgcccttct
      601 ggggcctcaa caagtaccac cagcggcatt ctttgtgggc caaggtggat ggggaccgca
      661 tgatcctgcg catgaagggc ggcgacagca attactccat ctccaccaag gacttcaacc
      721 gcgagttgaa ggccctgccc atctccttct ggcccaaggc cctgccccag acgctcttct
      781 gccggctgta cctgtgcgat tacttcatcc acggcgtggg cggcggcgcc tacgagcggg
      841 tgaacgacct gctgtgggaa aaggtcttca agtccaagcc cgtcgatttc ggcgtggtga
      901 ccgccacctg gaaggtggac ccggtccaga gcgacaagat cgcggagatc gtgcggcacg
      961 aggagcggat ccttttctgg gaacgcgcgc tgtcgaagaa cccggagacc ctcttcaccc
     1021 gccgggaagc ctgggagaag gaactgcccg agttcatccg caaggaattc gcccccctgg
     1081 ccgacgaccc cgaactggcc aagctggccc aggaaaagac ccgctgcgtg gagtcgctca
     1141 aggacccggc caaaaagccg gccgccgcca agcgcatcgg cgagttgaac caggcgctgg
     1201 cgggccgtta cgacccgctg gtgaaggcgc tggaaaaggg gttgatggac atcgagaagc
     1261 tcaagaaggt gcaggaggcc ctggacttcc gggaataccc cttcttcgcc cattcgccca
     1321 aaatcttccc cgaaatgaaa aagaggctcg aggagtcgct ggcctgaccg ggccggggat
     1381 cggtttttcc aggggcaaag ggtttgtttt tctttgcgt
//
LOCUS       DASTJJ010000637         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1556416, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000637 DASTJJ010000000
VERSION     DASTJJ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556416"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFR02_07790"
     CDS             <1..407
                     /locus_tag="VFR02_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870380.1"
                     /translation="DRSYTTPGGGGTPSPTPGGAVTISVSTGTSGASASGYVYTSSAG
                     SNGPDGLLTLTASVGDTIQLPGSGLHPLYFDAGSSTCIYSGSTGNQTYVFPSAGTYYF
                     HCGNHATACGGGGNAACGSTSCTSMAGVVTVN"
     gene            520..1083
                     /locus_tag="VFR02_07795"
     CDS             520..1083
                     /locus_tag="VFR02_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9870381.1"
                     /translation="MDFNDGQWRAWSDLMKRTQAGDGESYGRLLAEISPLVLNFVRKR
                     VYDRQQVEDVAQEVLLTFHKAKHTYRAELPFGPWFFTVIRNAVWSALQKNRRLAGAEL
                     LMGDFPEAAAPEAGEDQMDDRLLRALEGLPDIYRQAVDLLKFKGMSVEQAAAQLGVSQ
                     VALRVRAHRGYALLRKKLKAPQGKKND"
     gene            1076..1771
                     /locus_tag="VFR02_07800"
     CDS             1076..1771
                     /locus_tag="VFR02_07800"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9870382.1"
                     /translation="MTDPTPSKSAWDAMPESRRRLIEGLAAGHRPFRPAPSVPRQWLV
                     WLGFALVTVGAALSFIGPQFELRERLSDPASAGFLALCFALAAFMAWMGIASSKPDHP
                     FGRGPWILAGVLLVLLFAMPFLFFDRDSLSRVFAENLADGWFCARTVVLVAFPSWVML
                     GWLASRNAAFRPGWTGAWLGGSAFLLGAGTIQLHCAHWETCHMLVDHLLPMAALIFIP
                     IWVGSRWFSRWRR"
BASE COUNT          298 a          668 c          688 g          379 t
ORIGIN      
        1 tggaccgcag ctataccacg ccgggaggcg gcgggacgcc ctcgcccacc ccgggagggg
       61 ccgtgaccat ctcggtctcc accggcacca gcggcgcttc ggcctcgggg tacgtctaca
      121 cctccagcgc gggttccaac ggcccggacg gcctgctgac cctgaccgct tcggtggggg
      181 acaccatcca gctgccgggt tcgggcctgc atcccctcta tttcgacgcg ggcagctcca
      241 cctgcatcta ttcgggctcc accggcaacc agacctacgt ctttccctcg gccgggacct
      301 attatttcca ctgcggcaac cacgccacgg cctgcggcgg cgggggcaac gcggcctgcg
      361 gctcgaccag ctgcacctcc atggcggggg tggtgacggt caattaggcc tgcggcgtcc
      421 ggggtggatc gtccttgtgc ttccggacgc ccttctttaa agtaacgggg ttggatgggg
      481 aaatcggcgg tcccgatcgg gccaaaggag cttccaacct tggatttcaa cgacggccaa
      541 tggcgggcct ggagcgacct gatgaagcgg acccaggcag gcgacgggga gtcctatggg
      601 cgcctgctgg cggagatcag ccccttggtg ctcaacttcg tgcgcaagcg ggtctacgac
      661 cgccagcagg tggaggacgt ggcccaggaa gtcctgctga ccttccacaa ggccaagcac
      721 acctaccgcg ccgagctgcc cttcgggccc tggttcttca cggtgatccg caacgcggtc
      781 tggtccgccc tgcagaagaa ccgccggctc gcgggggcgg agctgctgat gggggatttc
      841 cccgaggcgg ccgcgcccga ggccggcgag gaccagatgg acgaccggct gctccgggcc
      901 ctggagggcc tgccggacat ctaccgccag gcggtggacc tgttgaagtt caagggcatg
      961 agcgtggagc aggcggcggc ccaattgggg gtctcgcagg tcgccctccg ggtgagggcc
     1021 caccggggat acgccctctt gaggaaaaag ttgaaggccc cacagggaaa aaagaatgac
     1081 tgacccgacc ccatccaaga gcgcctggga cgccatgccc gaaagccgcc ggcgcctgat
     1141 cgaaggcctg gccgccgggc accggccttt ccgcccggcc cccagcgtgc cgcgccagtg
     1201 gctggtgtgg ttgggtttcg ccctggtgac ggtcggcgcg gcgctgtcct tcatcgggcc
     1261 gcagttcgag ctacgggagc ggctctcgga ccccgcctcg gcgggcttcc tggcgctctg
     1321 cttcgccctg gcggccttca tggcctggat gggcatcgcg tccagcaagc cggaccatcc
     1381 cttcggccgg gggccctgga tcctggccgg ggtcctgctg gtgctgctct tcgccatgcc
     1441 cttcctgttc ttcgaccggg acagcctctc gcgggtcttt gccgagaacc tggccgacgg
     1501 ctggttctgc gcccgcacgg tggtgctggt ggctttcccc tcctgggtca tgctgggctg
     1561 gctggcgtcc cgcaacgcgg ccttccgccc cggctggacc ggggcctggc tgggcgggtc
     1621 ggctttcctg ctgggcgctg ggaccatcca gctgcattgc gcccattggg agacctgcca
     1681 catgctggtc gaccacctgc tgcccatggc ggccttgatc ttcatcccga tctgggtggg
     1741 gagccgttgg ttctcgcgct ggcgccgcta gtcctggacc tgttgcgaaa aggatattag
     1801 gttcgaggac ggggccatct tcgcagtgat gacggctgat ggggaccgat gaacctcgat
     1861 cttgaccggc ttcgatccac atcagcgttc atccgcattc atcattgcaa aaggatcttt
     1921 gctttgggga tggggcctgt ctttcatcaa tgttcatcat tgccaaagga tgtttgttgg
     1981 gaggtgggaa gcccatcttc gcagtgatga cggctgatgg ggaccgatga acc
//
LOCUS       DASTJJ010000638         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_699827, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000638 DASTJJ010000000
VERSION     DASTJJ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699827"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFR02_07805"
     CDS             <1..394
                     /locus_tag="VFR02_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9870383.1"
                     /translation="SDRPSFLIGLVQGLCLPFRGFSRSGATISAGLLLGVEKRTAEEF
                     SFALAVVLTPAVIAKEAWRLYKAQGLSGLSWDSLTPDLLGLVLSFLAGLLALKWLSGW
                     LERGRWGWFGFYCLGASAAVAALAWTGR"
     gene            473..946
                     /locus_tag="VFR02_07810"
     CDS             473..946
                     /locus_tag="VFR02_07810"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET9870384.1"
                     /translation="MEPRLVERKEMRVVGLRTRTRQLNEANLKGARIPALWDRFLTER
                     SGARIKHRLDPDVVLGVYSAYENDYLGEYDLLVGAQVGEIDTPPGFSSLIILPSKYLV
                     FKAKGQMPRAVSQTWAEIWDYFADSRHPERRAYTADFEWYHAAKPSEVDIFVSVK"
     gene            953..1486
                     /locus_tag="VFR02_07815"
     CDS             953..1486
                     /locus_tag="VFR02_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870385.1"
                     /translation="MPLPRKPSHRPALASAALALLLFGSPVQAGEVQWKRSPIQTQAS
                     FWGLEYRLAGDPLKDFQDFSDAMTPLGDTRVDRLLARSQDSALWARLGSLVGGVGLGW
                     GGFHLLFDDSPSRHGANTAILLTGVGVDLLASLFSEDSQAAKYNAVQRYNQVVRGEDD
                     SLPPAPKDDKGLLPPTQ"
BASE COUNT          279 a          580 c          549 g          337 t
ORIGIN      
        1 gtcggaccgt ccctcgttcc tcatcggcct cgtccagggg ctctgcctgc ccttccgggg
       61 attttcccgg tccggggcca ccatctccgc cggcctgttg ttgggggtgg aaaaacggac
      121 cgccgaggaa ttcagcttcg cgctggcggt ggtcttgacg cccgccgtca tcgccaagga
      181 ggcctggcgc ctgtacaagg cccagggcct ttccggactt tcctgggaca gcctcacgcc
      241 cgacctgctg ggcttggtcc tcagtttcct ggcggggctg ctggccttga agtggctctc
      301 ggggtggctg gaacggggcc gctggggatg gttcggtttc tattgtctcg gcgcctcggc
      361 ggccgtggcg gccctggcct ggacgggccg ttaaggggct ctttcccgga aaaccgctgg
      421 cgttccgggc gcgcggtttt tatcatggac ccggcacttt tgggaggccc gtttggaacc
      481 ccggttggtg gaacgcaaag aaatgcgggt ggtgggcctc aggacccgca cccggcaatt
      541 gaacgaggcc aacctgaagg gcgcccgcat ccccgccctt tgggaccgct ttctcacgga
      601 aagatcgggg gcccggatca agcaccggct ggacccggac gtcgtcctgg gagtctacag
      661 cgcctacgag aacgattact tgggggaata tgacctgctg gtgggggccc aggtggggga
      721 gatcgacacg cccccgggtt tttccagcct catcatcctt ccgtccaagt acctggtctt
      781 caaggccaag ggccagatgc cccgggccgt ttcccagacc tgggccgaga tctgggacta
      841 tttcgccgac agccggcatc ccgaacgccg ggcctacacg gcggatttcg agtggtacca
      901 cgcggccaag ccctcggaag tcgacatctt cgtgtccgtg aaataaggtc ccatgcccct
      961 tccccgaaaa ccttcacacc ggccggccct ggcgtcagcg gccctggccc tattgctctt
     1021 cggttccccc gtccaggccg gggaggtgca gtggaaaagg tcccccatcc agacccaggc
     1081 ctctttttgg ggactggaat accggctggc cggcgatccc ctgaaggatt tccaggactt
     1141 ctcggacgcc atgacgcccc tgggggacac gcgggtcgac cggctgttgg cccgatccca
     1201 ggactccgcc ctttgggcgc gcctgggttc cctggtggga ggggtgggcc tgggctgggg
     1261 cggcttccac ttgctgttcg atgactcgcc ttcccggcat ggcgccaaca ccgccatcct
     1321 gctgaccggg gtgggggtgg acttgctggc ttcccttttt tccgaggaca gtcaggcggc
     1381 caaatacaac gcggtccagc gctacaacca ggtggtccgg ggcgaggatg attccctgcc
     1441 gcccgcgcct aaggatgaca agggcctgct gcctcctacc caataaaaag accccgccgc
     1501 cggtcttcca tcactcaatt cccaagaccg gccaatgggc catccaccag gcagttccaa
     1561 taaaaaaggc cctgccgccg gtcctccgtc actcagttcc caagaccggc caatgggcca
     1621 tccaccagat agttccaata aaaaaggccc cgcggaaacc cgcggggcct tgtctttgag
     1681 gactagcgct tattcctggg gcaggctgga caccaccatc gtccggtccg tcttggtccc
     1741 ggact
//
LOCUS       DASTJJ010000639         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_946032, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000639 DASTJJ010000000
VERSION     DASTJJ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946032"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..242
                     /locus_tag="VFR02_07820"
     tRNA            168..242
                     /locus_tag="VFR02_07820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Gln,seq:ttg)
     gene            822..1673
                     /locus_tag="VFR02_07825"
     CDS             822..1673
                     /locus_tag="VFR02_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9870386.1"
                     /translation="MGLIRVILLAGSLFSAPLARAATVETCDQYRGVTVGPYVIQTDY
                     WNQGQCPGTQCMSIDDQTGVFTVTKATYQCGYSVGGYPSILYGKAFGGESPGSGLPAR
                     VDSLKCVHSSWTFHPSDTGRWDAAYDIWLCPDNQCGPGGFPGGAEVMVWLDYRNTNGW
                     QVDQGPVTLNGMPWEVWRWDVDDAGGKRTYVAYLAKTKTDSVKNLDLLEFLKDSHARG
                     YLKPSWYLYAVLAGVEIASGGVPFTSESYSVSLNGDCGVAPQILPIPASPTPTPDLTP
                     EDIPPPP"
BASE COUNT          377 a          594 c          556 g          389 t
ORIGIN      
        1 gaaggcagcg gcgtgatctt gggcggcggg gtctcttcgc tcataaggac agcctagcac
       61 ggcccatttt cggcctcaac gcccatccga aaggcccctt tgtgcttgcc cccggcccca
      121 agctgaatta aattacagcc ctgcctttcc cttccttaca acctgattgg ggcgtcgaca
      181 agcggtaagt caccagattt tggatctggc aatcgtaggt tcgaatccta ccgccccagc
      241 cactcgactc gctgcgctcg ctcgtggcag gccataaggc ctgacatggg cgggcaagcg
      301 atatttcgag gcgagtgccc tgagcgaccg tagggagtcg aatgggcttt agggtccttc
      361 ctctttcggg tctataattc cagccatgca ctatctttat atcctcgaaa tgtccgatgg
      421 acgtttatac gttggtgtta cggtggacct ccaacgtcga cagtctcaac acggccgagg
      481 ggatgcggct acccgcacca cccacatttt tggggctggg ctcattcttt atcacgagac
      541 ttttccggat cagaagtcgg ctttcaaacg cgaacatcag gtcaaaaggt ggtcccatgc
      601 caagaagtta gctttgataa agggggataa agccgagctg aagcgattgg ccaaaaggaa
      661 gaaataggtc cgatattctc cgctcctgcc actcgacttg ctccgctcac ccaggtcagg
      721 ccataagacc tgacatgggt gagcggagcg atccattaaa accaagctcc cgaagcttcc
      781 cggggttaaa atagaaagaa cgcaatgagg aaggggttcc catgggtctt atccgcgtga
      841 tcctgcttgc cggttccttg ttctccgcac ccttggcccg ggccgcgacg gtggagacct
      901 gcgaccagta tcggggcgtg acggtcgggc cctacgtcat ccagaccgat tattggaacc
      961 agggccagtg ccccgggacc caatgcatga gcatcgacga ccagaccgga gtctttaccg
     1021 tcaccaaagc cacttaccaa tgcggctaca gcgtgggcgg ttatccttcg atcctctacg
     1081 gcaaggcttt cgggggtgag agcccaggca gcggcctgcc ggcccgggtg gattccctca
     1141 aatgcgtgca cagcagttgg accttccatc cctcggacac cggccggtgg gacgcggcct
     1201 atgacatttg gctctgcccc gacaaccaat gcgggccggg cggtttcccc ggcggggccg
     1261 aggtgatggt gtggttggat taccggaaca ccaacggatg gcaggtcgac caggggccgg
     1321 tcaccctcaa cggcatgccc tgggaggtct ggcgctggga cgtggacgac gccgggggga
     1381 agcggaccta cgtggcctac ctggcgaaaa cgaagaccga ttcggtgaaa aacctcgacc
     1441 tgctggagtt cctcaaggat tcccatgccc ggggctatct caagccctcc tggtacctct
     1501 acgcggtcct ggccggggtc gagatcgcct cgggcggcgt tccctttacc agcgaaagct
     1561 attcggtctc cctgaatggg gactgcgggg ttgcacccca gatcctaccc atccccgctt
     1621 cgcccacccc gaccccggac ctgacgccgg aggatatccc cccgccgccg tgatgggcga
     1681 aggtttttcg ggcccctgtc cccatccggt agatactgcc ccccgcctgg gtaaatgcta
     1741 gaatcacacc attcggcgac tttcccgtcc atgcggagtc cctatgtcca tgtcttccct
     1801 ggaaaagatc aagaaacccc tcgaacgccg gcggttcctg ggcggtgaga gcctgatgaa
     1861 ggatccctct tgaaacggcc gtccccctcg ctctggatcg tggtggccgg ggtcac
//
LOCUS       DASTJJ010000640         1983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1434119, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000640 DASTJJ010000000
VERSION     DASTJJ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434119"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFR02_07830"
     CDS             complement(<1..725)
                     /locus_tag="VFR02_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET9870387.1"
                     /translation="MKEFFQLKDGQVGECPAAAANVVVYTRPDEAERQEILGTLRLDP
                     HALESAQDPDEISRMECLQDDLYVIWKRPNNTSFAQQFKLEVSSIGFYLQRDRLTVIL
                     SEGIMPFKEKEFQGAATLADLLLRSLQHTIHHFLGHLKAIKQLTGEIQTKLNASLGNQ
                     YLLQMFTLGENLIYYIDALEANATVLAKLHKEWERVGFSKAEVGLMEDITIEHHQCAK
                     QAEIYSTVLSGLMDARASIINNN"
     gene            complement(814..1476)
                     /locus_tag="VFR02_07835"
     CDS             complement(814..1476)
                     /locus_tag="VFR02_07835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9870388.1"
                     /translation="MIYAIGDIHGCLKQLQDLLAQLPLGPDDELVFLGDYIDRGPDSK
                     GVLDYLLKERQPHWHFLRGNHEQMLLDWLGTPNPLAASNWLLNGGHQTLQSYIPKDKM
                     DEVRGEGIHVLLQSYIPHTHVEFLNALPLTYDTPEAFFCHAGINLDKPLADQEADDLL
                     WIRRKFLQDPRPTPKLVVHGHTPVEKLDLTRDRINLDTGCVYGGALTALALPERKAYQ
                     AK"
     gene            complement(1473..>1983)
                     /locus_tag="VFR02_07840"
     CDS             complement(1473..>1983)
                     /locus_tag="VFR02_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870389.1"
                     /translation="GTGSYVLAQAKLKGFPVIKTINQYFKQSSDEIDFDQVAGNLGMK
                     DKVFSYTANCTGKVGQARVVGGIDCVKLAYSPDMKVQCDIKQEFVNSDSVKSGLPPAL
                     QGQVRHLDWLADSQGNIPVDLKFTGVAAENHYNWDTGRIMKNVGDHVGSALKDQAQDL
                     GKKLQGLFH"
BASE COUNT          339 a          608 c          613 g          423 t
ORIGIN      
        1 atgttgttgt tgatgatgct ggcccgcgcg tccatcaagc ccgacagcac ggtggagtag
       61 atctcggcct gtttggcgca ctggtggtgc tcgatggtga tgtcctccat caaccccacc
      121 tcggccttgc tgaaccccac cctttcccat tccttgtgca gcttggccag cacggtggcg
      181 ttggcctcca aggcgtcgat gtagtagatg aggttctcgc ccagggtgaa catctgcagc
      241 aggtactggt tccccaggga ggcgttgagt ttggtctgga tctcgccggt gagctgcttg
      301 atggccttca agtgccccag aaaatggtgg atggtgtgct gcaggctgcg caagagcagg
      361 tccgccaggg tggccgcgcc ctggaattcc ttttccttga agggcatgat cccctcgctc
      421 aggatgaccg tcagccggtc gcgctgcagg taaaagccga tggacgacac ctccagcttg
      481 aattgctggg cgaaggaagt gttgttgggc cttttccaga tgacgtaaag gtcgtcctgg
      541 aggcactcca tgcgggagat ctcgtccggg tcctgggccg attccaaggc gtggggatcc
      601 aggcgcaggg tgcccaggat ctcctgtcgc tccgcctcat cgggacgggt atagaccacc
      661 acattggccg cggcggccgg gcactccccg acctgtccgt ccttgagttg gaaaaattcc
      721 ttcatgcgcg ttcccgtggg ttcaaaatcg gccggtccgg ccgtctccgt gacgcccggg
      781 cgacccgggg gtcacgaccg ggtaaaactt ccctcacttg gcttgatagg ctttccgctc
      841 cggcagggcc aaggccgtca aagcgccccc gtagacgcaa cccgtgtcca gattgatgcg
      901 gtcccgggtg aggtccagtt tttccaccgg cgtgtggccg tgcaccacca atttgggcgt
      961 gggacggggg tcctggagga atttgcggcg gatccacaac aggtcgtcgg cctcctggtc
     1021 ggccaggggc ttgtccaggt tgatccccgc gtggcagaaa aaagcctcgg gggtgtcgta
     1081 ggtcaggggc agggcgttca ggaattccac gtgggtgtgg gggatatagg actggagcag
     1141 cacatggatc ccctcgcccc gcacctcgtc catcttgtcc ttcgggatgt aggattgaag
     1201 ggtctggtgg ccgccgttca agagccaatt ggaggccgcc agggggttgg gcgtgcccag
     1261 ccaatccagc agcatctgct cgtggttgcc gcgcaggaaa tgccaatggg gttggcgttc
     1321 cttcagcagg taatccagca cgcccttgct gtcgggcccg cggtcgatgt agtcgcccaa
     1381 aaagaccaac tcatcgtccg gccccagggg caactgggcc agcaggtctt ggagctgctt
     1441 caggcagccg tggatgtccc caatggcgta gatcaatgga acaacccctg cagcttcttg
     1501 cccaggtcct gggcttggtc cttcagggcc gagcccacat ggtcccccac gttcttcatg
     1561 atccggcccg tatcccagtt ataatggttc tccgccgcca cgcccgtgaa cttgaggtcc
     1621 accgggatgt tcccctggct gtccgccagc cagtccagat gcctcacttg accctggagc
     1681 gccgggggca acccggattt caccgaatcc gaattgacga actcctgctt gatgtcgcac
     1741 tggaccttca tgtccggcga ataggccagc ttgacgcaat cgatcccgcc cacgacacgg
     1801 gcctgcccga ccttgcccgt gcaattggcc gtataggaaa agaccttgtc cttcatcccc
     1861 aggttccccg ccacctggtc gaaatcgatc tcgtcgctgg attgcttgaa atactggttg
     1921 atggtcttga tcaccgggaa acccttgagc ttggcttggg ccagcacgta gcttcccgtc
     1981 ccc
//
LOCUS       DASTJJ010000641         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_456107, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000641 DASTJJ010000000
VERSION     DASTJJ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456107"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFR02_07845"
     CDS             complement(<1..494)
                     /locus_tag="VFR02_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET9870390.1"
                     /translation="MKVPVLIAGGGLAGLACARELHLAGADHLLVERERELGGLCRTV
                     RTGGFTFDYTGHFLHFNKPEMRQWVLSLMGDRLKPRRRHAAVYSEGAYTEYPYQENNA
                     GLPPGVARENVLGYLEAQLRRRFQGVDPAKAPDFHTWCLQTLGPGLSRNFMFPYNAKL
                     WKFPL"
     gene            complement(491..>1042)
                     /locus_tag="VFR02_07850"
     CDS             complement(491..>1042)
                     /locus_tag="VFR02_07850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET9870391.1"
                     /translation="LAFVLVPLGGESGYVNLVASALAGALLGFLPFNYQPARIYLGDS
                     GALFIGFILAGVAMGESYAQTNVVALAAPLLILGVPLYDTILVMFLRLLKGKSMFQGS
                     PDHLALRLRFLGLRVKQVVGLLWAMTAAGGVGAFFLVRLSEKRAFVLLLVLFFAVTLW
                     TLFIAMVPVDRPAGKRPARRARR"
BASE COUNT          188 a          351 c          369 g          134 t
ORIGIN      
        1 agcgggaatt tccagagctt ggcgttgtag gggaacatga agttccgtga aagcccgggc
       61 cccagggtct gcaggcacca ggtgtggaag tcgggggcct tcgccgggtc gaccccctgg
      121 aagcggcggc gcaattgggc ctccaggtag cccagcacgt tctcccgcgc cacgcccggg
      181 ggcagccccg cgttgttctc ttggtaggga tattcggtgt aggcgccttc gctgtagacg
      241 gcggcgtggc ggcggcgcgg tttgagccgg tcccccatga gggaaagcac ccactggcgc
      301 atctcgggct tgttgaagtg caggaaatgg cccgtgtagt cgaaggtgaa gcccccggtc
      361 cgcacggtgc ggcaaaggcc gcccaattcc cgctcccgct ccaccagcag gtggtcggcg
      421 ccggccaggt gcagttcccg ggcgcaggcc aggcccgcca gcccgccccc cgcgatgagg
      481 acggggacct tcatcggcgc gcgcggcggg ccgggcgttt gcccgccggt ctgtccacgg
      541 gcaccatggc gatgaagagg gtccagagcg tgaccgcgaa gaaaaggacc agcaacagca
      601 cgaaggcccg cttctcgctg aggcgcacca ggaaaaaggc gcccacgccg cccgccgccg
      661 tcatggccca gagcaggccc accacctgct tgacccgcag ccccaggaac cgcagccgca
      721 gggccaggtg gtcgggcgag ccctggaaca tggacttgcc cttcaacagg cgcaggaaca
      781 tcaccaggat ggtgtcatag aggggcacgc ccaggatgag caggggcgcc gccagggcca
      841 ccacgttggt ctgggcgtag ctctcgccca tggccacgcc cgccaggatg aagccgatga
      901 agagcgcccc cgagtcgccc aggtagatgc gggccggttg gtaattgaag ggaaggaacc
      961 ccagcagggc gcccgccagc gccgaggcca ccaggttgac gtagcccgac tcgccgccca
     1021 ggggcaccag cacgaaggcc ag
//
LOCUS       DASTJJ010000642         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1067454, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000642 DASTJJ010000000
VERSION     DASTJJ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067454"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFR02_07855"
     CDS             <1..216
                     /locus_tag="VFR02_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET9870392.1"
                     /translation="GTVMLAAWVGGPVTGASMNPARSLAPALFEGRLGSLWIYLAGPC
                     LGAVAAALLYEAIRLEPAHSAATRSRR"
     gene            213..611
                     /locus_tag="VFR02_07860"
     CDS             213..611
                     /locus_tag="VFR02_07860"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9870393.1"
                     /translation="MKKILFLCVANSARSQMAEGLARHFHADKAEVQSAGSKPSRVNP
                     FAVTALGELGIDISGHRSKSVREIDPTTVDTVITLCAEEECPVFLGQAERLHWPLPDP
                     GREGTDGEKLESFRKVRDEIQRRLAGYFGD"
     gene            complement(608..892)
                     /locus_tag="VFR02_07865"
     CDS             complement(608..892)
                     /locus_tag="VFR02_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870394.1"
                     /translation="MEPNLRIVSQLTRILQQSVDKHFGFMRPNNRSLEAAREHAQQLL
                     DQYIDGLMKESPDLKKQWGAGHPFLVLCRVSLETRSYQVSITTKDNPKGL"
     gene            complement(1086..>1794)
                     /locus_tag="VFR02_07870"
     CDS             complement(1086..>1794)
                     /locus_tag="VFR02_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870395.1"
                     /translation="KTREPWLLVFPGVIFLSILPVQDLGVRYLLPAYPFLILLGGRAA
                     ARLWDWQPRTSRRAGKLLAVGLLAWQAATALLNNGNLVSYFNDLVPPGKKLYLLGDSN
                     LDWGQETGKMARFARKEGWTRVKLAQFGGLDPGFYGLKAEGWTKRDLQGPQPGWVYLA
                     NAALLQEGPVFEPDLKPMVESWMLTVPPTGRVGDTWYYWDVPGTPAADPTAPVDSRRP
                     MGARERGLIPASRSAGP"
BASE COUNT          324 a          539 c          594 g          337 t
ORIGIN      
        1 ggcacggtga tgctggccgc ctgggtgggc gggcccgtga cgggcgcttc catgaacccg
       61 gcccgcagcc tggcgccggc cctgttcgag gggcgcttgg gaagcctgtg gatctatctg
      121 gcggggccct gcctgggcgc ggtggcggcg gccctgcttt acgaggcgat ccggttggaa
      181 ccggcccatt cggccgcgac aaggagccgt cgatgaaaaa gatattgttc ctctgcgtgg
      241 ccaactcggc ccgcagccag atggccgagg gcttggcccg gcatttccac gccgacaagg
      301 ccgaggtcca aagcgccggg tccaagcctt cccgggtgaa ccccttcgcg gtgacggccc
      361 ttggggaact ggggatcgac atctcgggcc accggtccaa gtcggtgcgg gagatcgacc
      421 caacgaccgt ggatacggtc atcaccctct gcgccgagga ggagtgcccg gtctttttgg
      481 ggcaagccga gcggttgcac tggcccctgc ccgacccggg acgggaaggg acggacgggg
      541 aaaagttgga gagcttccgg aaggtgcgag acgagatcca gcgccggttg gccggttatt
      601 tcggggacta gagccccttg ggattgtcct tggtggtgat gctgacctgg taggaccggg
      661 tttccaggct cacccggcag agcaccaaaa aggggtggcc cgcgccccac tgtttcttca
      721 ggtcgggcga ttccttcatc aatccgtcga tgtactggtc cagcagttgc tgggcgtgtt
      781 cccgggcggc ttccaggctg cggttgttgg ggcgcatgaa accgaaatgc ttgtccacgc
      841 tttgctgcag gatgcgggtc aattggctga cgatgcgcag gttgggttcc atcgggtgtc
      901 cactccctca gatgcttttc agtacggaat catgcatttc gcaaagcaag gacaatagga
      961 acactgataa aagctgataa agcctgatct ttattttcaa agatcagatt tcatcagttt
     1021 ttatcattgt ttcaagttct ctttggcttg ctttgcaaaa gccatagttc cgtttcagta
     1081 taccctcaag gcccggcgga acgggaggcc ggaatgaggc cccgctcccg tgcccccatg
     1141 ggacggcggc tgtccaccgg ggcggtggga tcggcggcgg gggtccccgg gacatcccaa
     1201 tagtaccaag tgtcgccgac ccggcccgtg ggggggacgg tgagcatcca actttccacc
     1261 atgggcttaa ggtcgggctc aaagaccggg ccttcctgca acagcgcggc gttggccagg
     1321 tagacccagc ccggttgggg gccttgaagg tcccgcttcg tccagccttc agccttcagg
     1381 ccgtaaaagc cgggatccaa cccgccgaac tgggccaact tcacccgggt ccaaccttcc
     1441 ttccgggcga agcgggccat cttcccggtt tcctgccccc agtccaggtt cgaatcgccc
     1501 aagagataca gcttcttccc gggcggcact aggtcgttga agtagctgac caagttcccg
     1561 ttgttcaaaa gggcggtggc ggcctgccag gccaaaagcc cgacggccaa cagtttcccc
     1621 gcgcgcctcg aggtccgggg ttgccagtcc caaagccggg ccgccgcgcg gccgcccagc
     1681 aggatgagga agggataggc gggcaagaga tagcgcaccc ccaggtcctg cacgggcagg
     1741 atggaaagga agatcacgcc gggaaagacc aggagccagg gttcgcgggt cttt
//
LOCUS       DASTJJ010000643         1962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_821475, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000643 DASTJJ010000000
VERSION     DASTJJ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821475"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFR02_07875"
     CDS             <1..202
                     /locus_tag="VFR02_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9870396.1"
                     /translation="YLQAVMDLVFLEAGGWVIVDYKTDRDPGERREAYEKQIALYAEL
                     LAQATGLPVKETALLFLRETAR"
     gene            complement(223..>1962)
                     /locus_tag="VFR02_07880"
     CDS             complement(223..>1962)
                     /locus_tag="VFR02_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870397.1"
                     /translation="FRLLGPSIRSVWLYPILYSILALPLAYAAVRKYGSRSFSLAYLG
                     FLALGFWPILVSRFSIDIELCLVWEYLALAAFSSIWRAWDSPRGPGAAQACFLGLVTG
                     LGWYTSKFWLPCLGLAVVTIGAYGWGRPKAQGMRWWSLFMGSLFLCVSPLLAALASQD
                     FFSHLHSIFVLQPHADPITPQPFFTLSYLTAPFWGIPDYPFFTFGPFLGGFFNPLVTT
                     LFWLGLLELFSSRRARGRWAVLLVGVFFFAPLLFTQSLEMIRICIALPFLLFPVAMGF
                     QTLLAGMKAPGQKWACGAIFGLSLALDMFHLWVGYGAWAVPENTQGRSKSALAYQVDG
                     ILRKAQHPPGPGLIMTDFIPDAFDQTLRAGTYALNALIDPAWSPRQATWACVWVRREY
                     EDYLRRLFPGSRVYRLERVETLENGATSLFLIPINDQNRPLLLRWGKFHEALRPIYGM
                     GHPHDPAKARYALSRLYPLVKNDPFLRSIFWWKWVCLADFQPDYIDNQDVLEKFIIPQ
                     AREPFWRTLISYTYYNLGMCYGTRRDLKDARLMLAKANLYDPQLVPLQAEAKAMAYMV
                     LKLGGTPPSPALP"
BASE COUNT          498 a          534 c          631 g          299 t
ORIGIN      
        1 ctacctgcag gccgtcatgg acctggtctt cctggaagcc ggcgggtggg tgatcgtgga
       61 ttacaagacc gaccgggacc cgggggaacg ccgggaggcc tatgaaaaac agatcgccct
      121 ttacgccgag ctcctcgccc aagccaccgg ccttcccgtc aaggaaaccg ccctgctctt
      181 tttaagggaa acggcccggt agctgggccc cggggggata cttcaaggaa gcgccgggct
      241 gggaggggtc ccaccaagct ttaggaccat gtaggccatg gccttggctt cggcttggag
      301 cggcaccagt tgggggtcgt aaagattggc ctttgccaac atcaggcgcg cgtctttcag
      361 gtcccgccgg gtaccgtaac acattcccaa attgtaatag gtatagctga tgagggtcct
      421 ccaaaagggt tcccgggcct gcgggatgat gaatttttcc aggacgtctt ggttgtcgat
      481 gtaatcgggt tggaaatcgg ccaagcagac ccacttccac caaaaaatgg acctgaggaa
      541 cgggtcgttt ttcaccaaag gataaagccg ggaaagggcg tagcgggcct tggccgggtc
      601 gtggggatgg cccatcccgt aaataggccg gagcgcctcg tggaattttc cccaccgaag
      661 aaggaggggg cggttttgat cgttgatggg gatgaggaaa agggaagtgg ccccgttctc
      721 cagggtttcg accctttcca atcgataaac acgcgagccg gggaaaagcc gccgaaggta
      781 atcctcgtat tcacgtctca cccaaacgca agcccaagtg gcctgtcggg ggctccaagc
      841 cggatcgatg agcgcgttca aggcgtaggt cccagcccga agggtctggt cgaaagcgtc
      901 ggggataaag tccgtcatga tcaaccccgg accgggagga tgttgggctt tgcgaaggat
      961 cccgtcgacc tggtaggcca aggcggattt ggaccggccc tgggtgtttt ccgggacggc
     1021 ccaggccccg taacccaccc aaagatggaa catatccaaa gccagggaga gcccgaagat
     1081 ggcgccgcag gcccattttt gtccgggggc cttcatcccc gccaacaggg tttggaaccc
     1141 catcgcgacg ggaaagagga gaaaaggaag ggcgatgcag atccggatca tctccaggct
     1201 ctgggtgaac aacagggggg cgaaaaaaaa gacaccgacc agcaggacgg cccacctccc
     1261 ccgggccctc cgggaagaga acaactccag caggcccaac cagaacaggg tcgtgaccaa
     1321 ggggttgaag aatccaccca aaaaaggccc aaaggtgaaa aagggatagt ccgggatgcc
     1381 ccaaaatggg gccgttaggt aggaaagggt gaaaaaaggt tgcggggtga tggggtcggc
     1441 gtggggctgg aggacgaaaa tggaatgtag gtgggaaaaa aagtcctggg aagccagcgc
     1501 cgccagcagc ggcgaaacgc aaaggaacag gcttcccatg aacagggacc accaacgcat
     1561 tccttgggcc ttcggacggc cccatccata ggctccgatc gtcacgaccg ccaggccaag
     1621 gcaaggcagc caaaacttgg aagtgtacca acccagtccg gtgaccaagc ccagaaagca
     1681 agcctgggcg gcgcccggcc cgcgtgggga gtcccaagcc ctccaaatgg aggaaaaggc
     1741 ggccagggcc aggtattccc aaaccaagca cagctcgatg tcgatggaaa aacgggacac
     1801 caagatgggc caaaaaccca gcgctaaaaa accaagataa gcgagggaaa aggaccggga
     1861 accatatttc cggacggcgg cataggccaa aggaagggcc aggatggaat acaggatggg
     1921 atagagccat accgaccgga tggaaggccc cagcagcctg aa
//
LOCUS       DASTJJ010000644         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1189348, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000644 DASTJJ010000000
VERSION     DASTJJ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189348"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFR02_07885"
     CDS             complement(<1..404)
                     /locus_tag="VFR02_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870398.1"
                     /translation="MDPRSTRILLVEDNPGDALLLQAALRGKGDPTYALRRVERLRDC
                     LDCLSQEDFDIILLDLTLPDSTGLSTLRAVVEKRPRVPIVVLTGLGDKETAIRSLQAG
                     AQDFLLKDELNGPLLKRSISYALERFQLLAQAR"
     gene            complement(469..876)
                     /locus_tag="VFR02_07890"
     CDS             complement(469..876)
                     /locus_tag="VFR02_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870399.1"
                     /translation="MSTLAQKGGRFIHVLLVEDNPGDVTLIKEVFKTSKHLITVSRAQ
                     DGVEALDFLKDETPDLILLDLNMPKKSGFEVLAEIKADPRLAEVPVVVLTNSKLDTDV
                     SRAYESRANFYIVKPSDLEKLFVALRYVEATWL"
     gene            complement(1161..2396)
                     /locus_tag="VFR02_07895"
     CDS             complement(1161..2396)
                     /locus_tag="VFR02_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET9870400.1"
                     /translation="MTSHHSIAVLTVFAATYAGIAVGRVPGLKVNRAGIALLGAIGMM
                     VLAGVPTAQVVDWVNWPMIALLFGFFVISAQLRLSGFYDRAAHAISRRLDRPAFFLLA
                     LMAAAAGLSAFLNHDIVCLAFTPVVAQALLRERLDPVPFLVALALAGNIGAAATLIGN
                     PQDMLIGETAHLKFGAYMLWSLPPVLASLGAAYGIVWSLSRQRLRTAGKRAVPPKAPE
                     YPYDRAHTLKGLVILVLAVGLFFTSIPKEITALAAAGIHLASRKFRTEDLLGRVDWEV
                     LVLFMGLFAVTGAFQSTGYGDAAVAWLAREGFDLGRPLHLILAAAGLSNLINNSAAVM
                     FLLKVVDLSKPATAYTLALANSFGGSLLIAGSVSNLIVAQQARAMGVRLSFQDFAKLG
                     VPVTLASLALLGFWVAWAG"
     gene            complement(2470..>2815)
                     /locus_tag="VFR02_07900"
     CDS             complement(2470..>2815)
                     /locus_tag="VFR02_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET9870401.1"
                     /translation="GTHSTDPVDYPDVAESLARAVLGGAVERGVLLCGSGVGASVCAN
                     KIRGVRACLIEDPFSARQGVEDDHLNVIALGGRVQGVELAWNLVRTFLKARYSRAPRH
                     LRRLRKVAGLEK"
BASE COUNT          526 a          942 c          928 g          419 t
ORIGIN      
        1 cgggcctggg cgagcaattg gaaccgttcc agggcgtaac tgatcgagcg cttcaacagg
       61 gggccgttca gctcgtcctt caacaggaaa tcctgggccc cggcctggag cgaccggatg
      121 gccgtctcct tgtcccccaa ccccgtcagg accacgatgg ggacccgcgg ccgcttttcg
      181 accaccgccc ggagggtgga cagccccgtg gaatcgggca aggtgaggtc caacaggatg
      241 atgtcgaagt cctcctggga caggcagtcc aggcagtccc gcagccgttc cacccgacgc
      301 aaggcatagg tggggtcgcc cttcccccgg agggccgcct ggagcaacaa ggcgtccccg
      361 ggattgtcct ccaccagtaa gatccgggtc gatctagggt ccatgggccc ctcctccccg
      421 agcggccttc ggccgttcgg cgggccgccg acaagcgtcc tcggcgtcct aaagccaggt
      481 ggcctccacg taacgcaggg ccacgaagag cttctccagg tccgagggtt tgacgatgta
      541 aaaattcgcc ctggactcgt aggcccggct gacgtcggtg tccaacttgg agttggtcag
      601 gaccaccacc gggacctccg cgagccgggg atcggccttg atctccgcca acacctcgaa
      661 gccgctcttc ttgggcatgt tcaggtccaa caggatcagg tcgggggtct catccttcag
      721 gaagtccagg gcttccaccc cgtcctgggc ccgggagacc gtgatgaggt gtttggaggt
      781 cttgaacact tctttaatga gggtgacgtc ccccggattg tcctcgacca acaggacatg
      841 gatgaaacgt cctccctttt gggccaacgt cgacataatg cccccctttc gatcgtcagg
      901 cgggtgggaa agaagcgggg cggggcgttc aagcccggtc cggctccagc tcgattgttt
      961 taaatggggg gatactgtaa ggaacgaagc ggcaacttcg tggagggcgt tgtaaccttc
     1021 atgaaaattc tactcctaaa ggacgggaga agcccgggtc taatccgttc tatgggggaa
     1081 acccattacc ctgccctttg cgggggcgcc cttgggggcc gggagggtga aaccgcccaa
     1141 aagccgccac aagaccttgc ctatcccgcc caggccaccc aaaagcccaa cagggccagg
     1201 gacgccaggg tcacgggcac acccaacttg gcgaaatcct ggaaagagag gcggacgccc
     1261 atcgcgcggg cctgttgggc cacgatgagg ttggacacgc tccccgcgat caagaggctc
     1321 cccccgaaac tgttggccag ggccagcgtg taggccgtcg cgggcttgga aaggtcgacc
     1381 accttcagga ggaacatgac cgccgccgaa ttgttgatca ggttggacag gccggcggcg
     1441 gccaggatca agtggagggg acgccccagg tcgaacccct cccgggccag ccaagccacg
     1501 gccgcgtccc cgtaacccgt ggactggaag gccccggtca cggcgaagag ccccatgaag
     1561 agcaccagca cttcccaatc cacccggccc aacaggtcct cggtgcggaa cttccggctg
     1621 gccaagtgga tgccggcggc cgccagggcg gtgatctcct tggggatgga cgtgaagaaa
     1681 agtcccaccg ccaagaccag gatcaccagg cccttgagcg tgtgggcccg gtcgtaagga
     1741 tattcggggg ccttcggggg aacggcccgc tttccggccg tccgcagccg ctggcgggac
     1801 agggaccaga cgatgccgta ggccgccccc agggacgcca ggacgggcgg caggctccaa
     1861 agcatgtagg cgccgaactt caagtgcgct gtctcgccga tgagcatgtc ctgcgggttc
     1921 ccgatgaggg tggcggccgc gccgatgttg ccggccaggg ccagcgccac caggaaaggc
     1981 acgggatcca gcctttcgcg cagcagcgcc tgggccacca ccggggtgaa ggccaggcag
     2041 acgatgtcgt ggttcaggaa ggccgacagg cccgcggcgg cggccatgag cgccagcagg
     2101 aaaaaagccg gacggtccag ccgccgggag atggcgtggg ccgcccggtc gtagaagccc
     2161 gagagccgca gttgggccga gatgacgaag aacccgaaca gcaaggcgat catgggccag
     2221 ttgacccagt ccaccacctg ggccgtgggg accccggcca ggaccatcat gccgatggcc
     2281 cccaagaggg cgatgcccgc tcggttcacc ttcaagcccg ggacccgccc caccgcgatc
     2341 cccgcgtagg tggccgcgaa gaccgtcagg acggcaatcg aatggtggga ggtcatgcca
     2401 cccgccttcc ctccgggaac ttcacaaagg gagcatccat cgacgcgttc ccttccaccc
     2461 ggtccggcct catttttcca accccgccac cttgcgcagg cggcgcaggt gccggggggc
     2521 ccggctgtag cgggccttca ggaaggtgcg caccaaattc caggccagct ccaccccctg
     2581 gacccggccg cccagggcga tcacgttcag gtggtcgtcc tccaccccct gccgggccga
     2641 aaaggggtcc tcgatgaggc aggcccgcac gccccggatc ttgttggcgc agaccgaggc
     2701 ccccaccccg ctgccgcaga ggagcacgcc gcgctccacc gcgccgccga gcacggcccg
     2761 cgcgagcgac tcggcgacgt ccggatagtc cacggggtcc gtgctgtggg tgccg
//
LOCUS       DASTJJ010000645         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1800343, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000645 DASTJJ010000000
VERSION     DASTJJ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800343"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFR02_07905"
     CDS             complement(<1..857)
                     /locus_tag="VFR02_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9870402.1"
                     /translation="MGRKAAWVWAAAWLAAAPLGAMELDGTGTLGSADWMGALTRAYA
                     PLAVQAPLHYDAQESRHGIARWLDKGADFLVVTQPLSPADDKRAGTGVMRLPVGLTAV
                     VITYNLPGVPSGLRLTPRALAQIFSGEITRWDNSNLRVMNPGLTLPDLPIRVLHRDDE
                     NSLFDLFPAWLKDQDGSWPLADAGPDQRLKWPLGRGVRGDEGLLRALRETAGSIGAAD
                     YVFAAQRGLPAACLENDLGAYVAPTPESLTAAVGDFAQLPADFMVSVSRPRDAGAYPL
                     TSFEWVLARS"
     gene            984..1712
                     /locus_tag="VFR02_07910"
     CDS             984..1712
                     /locus_tag="VFR02_07910"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET9870403.1"
                     /translation="MKEYDYAQLNARFEAAPAEEVVRWAVGELSPLCMSTSFGAESAV
                     LLHLVTRIKPDIPVLFTNTGFHFKETLEHRDRLVEKLKLNLRELKPETPTEEFLAKNG
                     RLYERDPDACCAVNKIAPFEKGLRDYAGWISGIRRAQAATREKAQFVENYRGYLVKVN
                     PLLNWNGKMFWDYAKQYGLPYHPLWEKGYLSIGCSPETCTRPASADGGIRSGRWTGQN
                     KVECGIHTFLKIEKDQAREQHAPK"
     gene            1699..>2494
                     /locus_tag="VFR02_07915"
     CDS             1699..>2494
                     /locus_tag="VFR02_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870404.1"
                     /translation="MRRNDPRLVAAALTALLSPALSFASPGGGAPLGAVASSGEPSLG
                     AVSSSSEPPLGGVASSSEAPFHPFWTGECSVDYGAEDGGQNGGLVWLGGRETLNPKGG
                     FFEAGVFGRSFQLESLRAGAFGLQMGGGVGLGDLTPSLEVALEAGDSHADAIRVLIGI
                     DKALAPDLTLDFSLTGEVASHQGPLGALVGLPAGSAAEIDDLNAIVGAALKWAALPPL
                     DLSLSLQAESDETVKLQDPSHTLFNQVDTTEWIYGLSLGAVGRLDAH"
BASE COUNT          393 a          899 c          808 g          394 t
ORIGIN      
        1 gaacgcgcca agacccactc gaaggacgtc agggggtagg cccccgcgtc gcggggccgg
       61 ctgaccgaga ccatgaaatc cgccggcagt tgggcgaaat cccccaccgc ggcggtgagg
      121 ctctccggcg tgggggccac gtaggccccc aggtcgttct ccaagcaggc ggcgggaagc
      181 ccccgctggg ccgcgaagac gtagtcggcc gcgccgatgg acccggcggt ctcccgcagg
      241 gcgcgcagca agccctcatc gccccgcacg ccccggccca ggggccattt gaggcgttgg
      301 tccggtcccg cgtccgccag gggccagctc ccgtcctggt ccttgagcca ggcggggaaa
      361 aggtcgaaga ggctgttctc gtcgtcccgg tgcagcaccc ggatgggcag gtcgggcagg
      421 gtcaggccgg ggttcatgac gcgcaggttg gaattgtccc accgggtgat ctcgcccgag
      481 aagatctggg cgagggcgcg gggcgtgagc cgaagccccg acggcacgcc gggcaggttg
      541 taggtgatga ccacggccgt caggcccacg ggcaggcgca tcacccccgt ccccgcgcgc
      601 ttgtcgtcgg cgggtgacag gggctgggtc accaccagga aatcggcccc cttatccagc
      661 caccgggcga tgccgtgccg gctttcctgg gcgtcgtaat gcagcggcgc ctggaccgcc
      721 aggggggcgt aggcgcgcgt gagcgccccc atccagtccg cggatcccag cgtgccggtc
      781 ccgtccagtt ccatggcgcc caagggcgcc gccgccagcc atgccgccgc ccagacccaa
      841 gccgcttttc gccccatgct ttccacgcct ttccgccgat ccaaggccca accaggcccc
      901 cggcgccttt ccttgttttt gacccggccc gaccctatga tgattcggct tccttatata
      961 tcacaagcgg cccaagggga cccgtgaaag aatacgatta cgcccaattg aacgcccgtt
     1021 tcgaagcggc ccccgccgag gaggtcgtcc gctgggccgt gggggaactt tccccccttt
     1081 gcatgtccac ctccttcggg gccgaaagcg ccgtgctgct gcacctggtc acccggatca
     1141 agcccgacat ccccgtgctt ttcaccaaca cgggtttcca tttcaaggag accctggagc
     1201 accgggaccg cctggtggaa aaactgaagc tcaacctgcg ggagctcaag cccgagaccc
     1261 ccaccgagga attcctggcc aagaacggcc ggctttacga aagggacccg gacgcctgct
     1321 gcgccgtcaa caagatcgcc ccctttgaaa agggcctgcg ggactacgcg ggctggatct
     1381 cgggcatccg gcgcgcccag gccgccaccc gggaaaaggc ccagttcgtg gagaactacc
     1441 ggggctacct ggtcaaggtg aaccccctgc tgaactggaa cggcaagatg ttctgggact
     1501 acgccaagca atacggcctg ccctaccatc cgctctggga aaagggctat ctttccatcg
     1561 gctgctcgcc cgagacctgc acgcggccgg cctccgcgga cggcggcatc cggtccggcc
     1621 gctggacggg ccagaacaag gtcgaatgcg gcatccacac cttcctgaag atcgaaaagg
     1681 accaggcgcg ggagcagcat gcgccgaaat gatccccgcc tggtggcggc cgcgctgacg
     1741 gcgctgctct cccccgccct ttccttcgcc tccccgggag gcggagcccc cttgggggcc
     1801 gtagcttctt ccggtgaacc ctccttaggg gctgtatcct cctccagcga accccccttg
     1861 gggggcgtag cttcttccag cgaagccccc ttccatccct tttggacggg cgaatgctcg
     1921 gtggactatg gcgccgagga cggcggccag aacggcggct tggtgtggct gggcggccgg
     1981 gagaccctga acccgaaggg cggctttttc gaggccgggg tcttcggccg ttccttccag
     2041 ctggaatccc tccgggccgg cgccttcggc cttcagatgg gcggcggggt cggcctgggc
     2101 gacctgacgc cttccctgga ggtcgccctg gaggccggtg attcccacgc cgacgccatc
     2161 cgcgtgctca tcggcatcga caaggccctg gccccggacc tcacgctgga cttttcgctc
     2221 accggcgagg tggccagcca ccaggggccc ttgggcgccc tggtcgggct tcccgccggg
     2281 tccgccgccg agatcgacga cctcaacgcc atcgtgggcg ccgccttgaa atgggccgcc
     2341 cttcccccgc tggacctgtc cttgtcgctc caggccgaat ccgacgagac cgtcaagctg
     2401 caggatccct cccacacgct cttcaaccag gtggacacca ccgaatggat ctacgggctt
     2461 tccctggggg ccgtcggccg cctggacgcc cact
//
LOCUS       DASTJJ010000646         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_946448, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000646 DASTJJ010000000
VERSION     DASTJJ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946448"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFR02_07920"
     CDS             complement(<1..592)
                     /locus_tag="VFR02_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870405.1"
                     /translation="MRIKRYEAPTIQEALQKVKKDLGPEAVILYTKTFRKGGVMGMFG
                     KPMAEITAGIDLNLMDDMARRKTSASAGVVEGPETVALKPAPAPAAPAGMDPARAKVR
                     ALQRELNEMKTSSLAGSLRESGPMDSLSLLSEGFSKLRKKMAKQEVEDFLIQKVIKGM
                     MNEKVDPEKAGDVQEWLHRFVMGSMKIAPPMATSGFP"
     gene            complement(582..>1186)
                     /locus_tag="VFR02_07925"
     CDS             complement(582..>1186)
                     /locus_tag="VFR02_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HET9870406.1"
                     /translation="AHEFLTRQSVAKLLEGVKKETPAVVDEIIPSQLTIGELQKILQN
                     LLKEHLPIRDLESILEAVADHVRETKDTQLLTEFARRGLARSITKLYQAADGKIYCLT
                     LDPKFEKILMDSIQAAESGNAAALDPRLVQKMFRSLSAGIEKMAGTSRQPLVLCAAPV
                     RPYFKRMVERYIPHLTVLSFNELLPRTEIQSMGAIEANEN"
BASE COUNT          184 a          382 c          359 g          261 t
ORIGIN      
        1 tggggaaccc gctcgtggcc atgggcgggg cgatcttcat ggatcccatg acgaagcgat
       61 ggagccactc ctgcacgtcc cccgcttttt caggatccac tttctcgttc atcatgccct
      121 tgatgacctt ttggatgagg aaatcctcca cttcttgttt ggccatcttt ttgcgcagct
      181 tggagaagcc ctcggacaaa agcgacaggc tgtccatcgg tccgctttcc cgcagcgacc
      241 ccgccaggct ggaggtcttc atctcgttca attcgcgctg cagcgcccgc accttggcgc
      301 gggccgggtc catcccggcc ggggccgccg gcgcgggcgc gggcttcagg gccacggtct
      361 cgggcccctc caccaccccg gcggaggccg aggtcttgcg gcgggccatg tcgtccatca
      421 ggttcaggtc aatgccggcc gtgatctcgg ccatgggctt gccgaacatg cccatcaccc
      481 cgcccttgcg gaaggtcttg gtgtaaagga tcacggcctc cggacccagg tccttcttga
      541 ccttttgcag ggcttcttgg atcgtcgggg cttcgtagcg tttaattctc attggcctcg
      601 atcgccccca tggattggat ttcggtccgt ggcaaaagct cgttgaacga cagcacggtc
      661 aggtggggga tgtaccgctc caccatccgc ttgaagtaag gccgcaccgg cgccgcgcaa
      721 agcaccaagg gctggcggga ggttcccgcc atcttttcga tgcccgccga caggctccgg
      781 aacatcttct ggaccaaccg cgggtccaac gccgccgcgt tcccgctctc ggcggcctgg
      841 atggaatcca tcaggatctt ctcgaacttg ggatccagcg tcaggcagta gattttacca
      901 tcggcggcct ggtaaagctt ggtgatggac cgggccaggc cccgccgggc gaattcggtc
      961 aaaagctggg tgtccttggt ctcccgcaca tggtccgcca cggcctccag gatgctctcc
     1021 aggtcccgga tgggcaggtg ctctttcagc aggttttgaa ggattttctg cagttccccg
     1081 atggtcaatt gcgaggggat gatctcgtcc accactgcgg gcgtctcctt tttcacgcct
     1141 tccaggagct tggccacgct ctggcgggtc aggaattcgt gggcct
//
LOCUS       DASTJJ010000647         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_946469, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000647 DASTJJ010000000
VERSION     DASTJJ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946469"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFR02_07930"
     CDS             <1..360
                     /locus_tag="VFR02_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870407.1"
                     /translation="ITAQTLGPAQGPAEIEVKIADNGPGLSQEALQSVFDPFFVRSQN
                     PQDFGLHLMTCFFIVYHHGGKIEASTGPEPGKGALFTLRFPANPMAGAPALPGEQDFL
                     HRILLNENLWERLLAEN"
     gene            370..1713
                     /locus_tag="VFR02_07935"
     CDS             370..1713
                     /locus_tag="VFR02_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9870408.1"
                     /translation="MASITYPNGPKTAYPLQHLFALAKDPIGFISRLSREYGRMVHFP
                     MGGRPTYILNHPDLVQEVLATQHQNFTKGSGLIRLKQVLGDGLITSEDPLHLRQRRLS
                     QPAFHRQRIAAYGAVMSDYAYQARERWKEGETIDIEPETARITMAIVGKTLLNADVDG
                     EAHEIAEAVDTMRERFHIAVFPGMGLLDKLPLPHNFRFEKARRRLDETIYRMIEDRRS
                     SGADHGDLLSMLILARDTEGDQDGMTDRQVRDEAMTIFLAGYETITNTLTWTWYLLSQ
                     HPEIEKKFHDELDRALGDKVPRMDDLMRLPYTQKVLTEAMRLYPAAWMIAREPVEECR
                     VGGYTIPANAILFMCPYTIQRLPEYYPDPEKFDPERWEGKAKDSRPRFAYFPFGGGPR
                     QCIGEPYALMEGALILATLGQKWKFRLKEGHPVTIQANVTIRPKYGMPMVLEKRA"
     gene            complement(1783..>3309)
                     /locus_tag="VFR02_07940"
     CDS             complement(1783..>3309)
                     /locus_tag="VFR02_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9870409.1"
                     /translation="EPAPLVEPAGSGEPWGPALLASVAEAWHFSRLLKRAPLDPEGRA
                     GLDQSYAQFFSHLLDRLEAAGADLKGVAQWFRTSQGEVVDNDAVLAMVPLMELSLRHG
                     WTVVLFDPATEGLVNDCLGLWGAQGRQDLVEKAVMALAEGLGRSEAERRIALTHLMDA
                     RPWVDNPALLGKVLEQLCRLLAAEKNPGVYQSALLSAWDLVEPALQTHNEGSALSLLS
                     TLHFHADDDEPRPEFPERASIARHWLFERSTPALVRRMAVCAYQAGRLDHFPLLGEKA
                     APLLLDDYRDADAEEKKMLLGLFAGMGDPLRSALAERVADLSSAAELRALIPVLRTAG
                     MDAALSFQLSPWFSRGDRELKLNLIGLIEQIGDPEGGPALRLALFDDSEEIACAAARV
                     LGRIGFTQAVPVLVKACRLRQGRGPDHEKFTVEVCRALGRLGQASAIPFLEEVARRKP
                     LFRGGQASLPLRLAALEALGEIDQPPVWQFFESLAAEKNPELQEALDRIIHAKTETLS
                     "
BASE COUNT          592 a         1162 c         1072 g          483 t
ORIGIN      
        1 atcacggccc agaccctggg gcccgcccag ggcccggcgg agatcgaagt gaagatcgcc
       61 gacaacggcc ccggcctttc ccaggaagcc ctccagtcgg tcttcgaccc cttcttcgtg
      121 cgcagccaga acccccagga cttcggcctg cacctgatga cctgcttctt catcgtgtac
      181 caccacgggg gcaagatcga ggcctccacg ggccccgaac cgggcaaggg ggcgctcttc
      241 accctgcgct tcccggccaa tcccatggcc ggcgcgcccg cgctccccgg cgagcaggat
      301 ttcctgcacc ggatcctgct caatgagaac ctgtgggagc ggctgttggc ggagaactag
      361 gggacggaca tggcgtcgat cacctacccc aacgggccca agacggccta tccgctccag
      421 catcttttcg cgctggccaa ggaccccatc ggcttcattt cccgcctgtc gcgggagtac
      481 ggccgcatgg tccatttccc catgggcggc cgccccacct acatcctgaa ccatccggac
      541 ctggtgcagg aagtgctggc cacccagcac cagaatttca ccaaggggag cgggctcatc
      601 cggctcaagc aggtgttggg cgacgggctc atcaccagcg aggatcccct gcacctgcgc
      661 caacgccgcc tgtcccagcc ggccttccac cgccaacgca tcgcggccta cggcgccgtg
      721 atgtcggact acgcctacca ggcccgggaa cgctggaaag aaggggagac catcgacatc
      781 gagcccgaga cggcccgcat caccatggcc atcgtgggca agacgctctt gaacgccgac
      841 gtggacggcg aggcccacga gatcgccgag gcggtggaca ccatgcggga gcggttccac
      901 atcgcggtgt tcccgggcat ggggctcttg gacaaactgc ccctgcccca caatttccgc
      961 tttgagaagg cccgccgccg gttggacgag accatctacc gcatgatcga ggaccggcgc
     1021 tcctcggggg ccgaccacgg cgacctgctg tccatgctca tcctggcccg ggacaccgag
     1081 ggcgaccagg acggcatgac cgaccgccag gtgcgcgacg aggccatgac catcttcctg
     1141 gccggttacg agaccatcac caacaccctc acctggacct ggtacctgct ctcccagcac
     1201 ccggagatcg aaaaaaagtt ccatgacgag ctggaccggg ccttggggga caaggtgccc
     1261 cgcatggacg acctgatgag gctgccctac acccagaagg tgctcaccga ggccatgcgg
     1321 ctttatcccg cggcctggat gatcgcccgg gaaccggtcg aggaatgccg ggtggggggc
     1381 tacaccatcc ccgccaacgc catcctgttc atgtgcccct acaccatcca gcggctgccc
     1441 gaatactacc cggacccgga aaagttcgac cccgaacgct gggagggcaa ggccaaggac
     1501 agccgtcccc gcttcgccta tttccccttc ggcggcgggc cacgccagtg catcggggaa
     1561 ccctacgccc tcatggaagg cgcgctcatc ctggccaccc tgggccagaa atggaagttc
     1621 cgcctgaagg aaggccaccc cgtgacgatc caggccaacg tgaccatccg gcccaaatac
     1681 ggcatgccca tggtgctgga aaagcgggca taaaaaaacc cccgcgggtt ttcgcccgtg
     1741 ggggtgaaga aggggctggg aggtgaggcc ccggcccggt tttcaggaga gggtttcggt
     1801 cttggcgtgg atgatcctgt ccaacgcttc ctgcagttcg gggttctttt ccgcggccag
     1861 cgactcgaaa aattgccaga ccggcggctg gtcgatctcc cccagggctt ccagggccgc
     1921 caaacgcaac ggcagggagg cctgtccccc gcggaacagg ggcttgcgcc gggccacctc
     1981 ctccaagaag gggatggcgg acgcctgtcc caagcgtccc agggcccggc agacctccac
     2041 cgtgaacttc tcgtggtcgg gtccccggcc ttggcgcaac cggcaggcct tcacgagcac
     2101 gggcacggcc tgggtgaagc cgatgcggcc cagcacccgg gcggccgcgc aggcgatctc
     2161 ctccgagtcg tcgaacaggg ccagccgcag cgccggcccg ccttccgggt ccccgatctg
     2221 ctcgatgagg ccgatgaggt tcaacttcag ctcgcggtcg ccccgggaga accaggggga
     2281 caactggaag gaaagcgcgg cgtccatgcc cgcggtgcgc agcacgggga tcagggcccg
     2341 cagctccgcg gcggacgaga gatccgccac ccgctcggcc agcgccgacc gcagggggtc
     2401 ccccatcccg gcgaagagcc ccaagagcat cttcttttct tccgcgtcgg cgtcccggta
     2461 gtcgtccaac aacagggggg ccgccttctc gcccagcagg gggaaatggt ccagccgccc
     2521 ggcctggtag gcgcagaccg ccatgcgccg caccagggcg ggcgtggagc gctcaaaaag
     2581 ccaatgccgc gcgatggagg cccgctcggg gaactcggga cggggctcgt cgtcgtcggc
     2641 gtggaaatgc agggtggaaa gcaggctcag ggccgagccc tcgttgtggg tctggagggc
     2701 gggttccacc aagtcccagg ccgacagcag ggcgctttgg tagacgccgg gattcttctc
     2761 ggcggccaag agccggcaga gctgttccaa gaccttgccc agcagggccg ggttgtcgac
     2821 ccagggcctg gcgtccatca ggtgggtgag ggcgatgcgg cgttcggctt ccgagcggcc
     2881 cagcccctcg gccagggcca tcacggcttt ttccaccagg tcctgccgcc cttgggcgcc
     2941 ccaaagcccc aggcagtcgt tcaccaaccc ctcggtggcg ggatcgaaca gcaccacggt
     3001 ccagccgtgg cgaagcgaaa gttccatcaa gggcaccatg gccagcacgg cgtcgttgtc
     3061 caccacctcg ccctgggagg tgcggaacca ctgggccacg cccttcaggt ccgcgccggc
     3121 ggcttccagg cggtccagca gatgggaaaa gaactgcgcg taggattgat ccaacccggc
     3181 gcggccttcc ggatccaggg gggcgcgttt caagagccgg gaaaaatgcc aggcttcggc
     3241 caccgaggcc aggagcgcgg ggccccaggg ttccccggat cccgcgggtt ccaccaaggg
     3301 cgcgggttc
//
LOCUS       DASTJJ010000648         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_821745, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000648 DASTJJ010000000
VERSION     DASTJJ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821745"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1192
                     /locus_tag="VFR02_07945"
     CDS             32..1192
                     /locus_tag="VFR02_07945"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9870410.1"
                     /translation="MGTFDVLVIGAGGAGMMCAFQAAQRGRRTALFDHSPKPGAKILI
                     SGGGRCNFTNRGAGPDNYVSQNPHFCRSALARFTPEDFIALVEKHRIPYHEKKLGQLF
                     CDHSAQAIVDLLTGECGRAGVAFHMGKPLTEVRKIGEGFTVRTGAGDFQGKSLVVATG
                     GLSIPKVGATDLGYRLARQFGLKVVPTSPALDGFRFSPGDLKKFGDLTGVALDSRVTA
                     GGVCFRENILFTHAGLSGPAALQASLYWKAGEAIQVDLLPGVDGADWEAARPGPKQNP
                     AKALGRFLPHRLAERLVELEWPGHPGEKDWALWGEKLRDYALSPAATVGYSKAEVTRG
                     GVDTDELSSKTMESRKVPGLYFIGEVVDVTGWLGGYNFQWAWSSGWAAGQAV"
BASE COUNT          238 a          426 c          460 g          233 t
ORIGIN      
        1 gacggcgccg gacgccctgc tagaatgccg catgggaaca ttcgacgttt tggtcatcgg
       61 ggcgggcggc gccgggatga tgtgcgcctt ccaggccgcc caacggggcc gccggacggc
      121 ccttttcgac cattcgccca agccgggcgc caagatcctc atttcgggcg gggggcgctg
      181 caacttcacc aaccgggggg cggggccgga caactacgtt tcccagaacc cgcatttctg
      241 cagatccgcc ctggcccgct tcaccccgga ggatttcatc gccctggtgg aaaagcaccg
      301 gatcccctac cacgaaaaga agctgggcca gcttttctgc gaccactcgg cccaggccat
      361 cgtggacctg ctcaccgggg agtgcggccg ggcgggggtt gccttccata tgggaaaacc
      421 cctcacggag gtacgaaaga tcggggaggg attcacggtc aggaccggcg cgggggactt
      481 ccaggggaag tccttggtgg tggccacggg cggcctgtcc atccccaagg tgggggccac
      541 ggacctgggc taccggctgg cgaggcagtt cggcctgaag gtggtgccca cctcccccgc
      601 cctggacggc ttccgcttct cccccgggga cctcaagaag ttcggcgact tgaccggggt
      661 cgccctggac agccgggtca ccgccggcgg ggtttgtttc cgggagaaca tcctcttcac
      721 ccatgccggc ttgagcggcc cggcggccct acaggcttcc ctttattgga aggcgggcga
      781 ggccatccag gtcgatcttt tgcccggggt cgacggggcc gattgggaag cggcccggcc
      841 tgggcccaag cagaaccccg ccaaagccct cggccgtttc cttccccacc gcctggccga
      901 gcgcctggtg gagctggaat ggcccgggca tccgggtgaa aaagactggg cgctttgggg
      961 tgaaaaatta agggactatg ccctatcccc cgctgccacc gtgggctatt caaaggcgga
     1021 agtcacccgg ggcggggtcg atacggacga gctgtcgtcc aagaccatgg aaagccgtaa
     1081 ggtcccgggg ctttatttca tcggcgaagt ggtggacgtg accggctggc tgggcggcta
     1141 taacttccag tgggcctggt cctcgggctg ggccgcaggc caagcagtat aacgggtggt
     1201 tgaaaaaagc catagaacgg ctttttcgta attggtcttt accccggggc cgttgacaag
     1261 gcgccgcaaa caggcgcctt gtgaaaatat tcattgaaag acccagcccg agggaaccga
     1321 gggcgtctcg tctggctgca cggagggcgc ccaacgc
//
LOCUS       DASTJJ010000649         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1557285, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000649 DASTJJ010000000
VERSION     DASTJJ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557285"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFR02_07950"
     CDS             complement(<1..848)
                     /locus_tag="VFR02_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870411.1"
                     /translation="MRALSLSTLAFILMALGAGCGLQVHFTQVSSPRYWAVENKTQAQ
                     LNRIQAAAQGQQVPYFLADGLAVNDDLIRRLNGRFRDTSNGAPDISPAQYAFLLTMIN
                     GNEQALADILADPAEWEKSFAAQDAFARSDRKPAFHAFNDAARRLRFIIYLNTRLKAD
                     AARARALSDAGHISPDQARDLQDNLNDVQNKALEDYQANGALDLTADQIFQLRRMLAD
                     AYEPLIRPQGPPAYLGGSFQPGTGFSGGGYTAPGGSYYGGGYQSVGRSYNPPPKTGHP
                     APTATPT"
     gene            complement(984..>1409)
                     /locus_tag="VFR02_07955"
     CDS             complement(984..>1409)
                     /locus_tag="VFR02_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9870412.1"
                     /translation="SGERHLKKRPWAATAIIGGAILVGVLWHAASVENWSPHFNEQPL
                     PASVVGATEGPVAEGAQIFFDRGCINCHAIQGHGGRKGPDLTYIANELDESQIILRIA
                     NGGHNMPSFASIIKADEMTKLVAFLETRRREPLPYAPNK"
BASE COUNT          218 a          409 c          543 g          239 t
ORIGIN      
        1 gtcggggtgg cggtgggcgc ggggtggccg gtcttgggcg gcgggttata ggaccggccg
       61 acggattggt agccgccgcc gtaataggac cctccgggcg cggtatagcc gccgcccgag
      121 aagcccgtcc ccggctggaa ggaaccgccc aagtaggccg gcgggccttg gggccggatg
      181 agcggctcat aggcgtcggc cagcatgcgg cgcagctgga agatctggtc ggcggtcagg
      241 tccagcgccc cgttggcctg gtaatcctcc agggccttgt tctgcacgtc gttcaaattg
      301 tcctgcaggt cgcgggcttg gtcggggctg atgtggcccg cgtcggacag ggcccgggcc
      361 cgggcggcgt cggccttgag gcgggtgttc aagtagatga tgaaccgcag gcggcgggcg
      421 gcgtcgttga aggcgtggaa ggccggcttg cggtcggacc gggcgaaagc gtcctgggcg
      481 gcgaagcttt tttcccattc ggcggggtcg gccaggatgt ccgccagggc ctgctcattg
      541 ccgttgatca tggtcaaaag gaaggcgtac tgggcggggg aaatgtccgg ggcgccgttg
      601 gaagtgtccc ggaagcggcc gttcagccgc cggatgaggt cgtcgttcac cgccaggccg
      661 tcggccagaa aataggggac ctgttggccc tgggcggcgg cctggatccg gttcaattgg
      721 gcctgggtct tgttctccac cgcccaatag cggggtgagg agacctgggt gaaatgcacc
      781 tgcaggccgc agccggcccc cagggccatc aggatgaagg caagcgtcga cagggacaag
      841 gcgcgcatgg gaacctcgcg gagaaaggat gggaatccgg ggttagtttg cccccgaagg
      901 ggggctttgg caagccggaa ggccgccgga aaataaaaag gccccatccg cgcggcgcgg
      961 atggggcctt tggaacggcc tttttatttg ttcggggcgt agggaagggg ttcgcgcctg
     1021 cgggtctcca ggaaggcgac cagcttggtc atctcgtcgg ccttgatgat gctcgcgaag
     1081 gacggcatgt tgtgcccgcc gttggcgatg cgcaggatga tctgcgactc gtccaactcg
     1141 ttggcgatgt aggtcaggtc cggccccttg cggccgccgt ggccctggat ggcgtggcag
     1201 ttgatgcagc cgcggtcaaa aaagatctgg gcgccctcgg ccaccgggcc ctcggtcgca
     1261 cccaccaccg aagccggcag gggctgctcg ttgaagtgcg gcgaccagtt ctccacggag
     1321 gcggcgtgcc agagcacgcc caccaggatg gccccgccga tgatggcggt ggcggcccag
     1381 gggcgcttct tcaagtggcg ctcgcccga
//
LOCUS       DASTJJ010000650         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_700803, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000650 DASTJJ010000000
VERSION     DASTJJ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700803"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..657)
                     /gene="frr"
                     /locus_tag="VFR02_07960"
     CDS             complement(136..657)
                     /gene="frr"
                     /locus_tag="VFR02_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9870413.1"
                     /translation="MKKAIESTKRDFSAIRTGRASSALVENVKVEYYGSLVPMNQVAN
                     IAVSDSRTLEIKPWDAQALGDIEKALLKSDVGVTPTNDGKLIRLSMPPLTEERRKEFV
                     KLVHRHAEEGRVGIRGIRQDANKHLDQLKKDKTLSEDEVKKGQDRIQKMTDQYTDEIK
                     KLSEHKEKEILEF"
     gene            complement(711..1445)
                     /gene="pyrH"
                     /locus_tag="VFR02_07965"
     CDS             complement(711..1445)
                     /gene="pyrH"
                     /locus_tag="VFR02_07965"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9870414.1"
                     /translation="MAASSPSAKKPLYKKVLLKISGEALAGSKGYGIDAGVVQAIAQA
                     VHGVHQKGVQVALVIGGGNIYRGADGEKMGMDRVSSDYMGMLATLINALALQNALEKV
                     GAYTRVQSAIEMSKVAEPFIRRRAMRHLEKHRIVIFAGGTGNPYFTTDTAAALRAIEI
                     GADAILKATNVDGIYSADPKKDPKAKKFKTLTYIDAINRRLKVMDTTALTLCMENKLP
                     IVVFSLQQKENIKRVIWGEPIGTLVR"
     gene            complement(1449..>1819)
                     /locus_tag="VFR02_07970"
     CDS             complement(1449..>1819)
                     /locus_tag="VFR02_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9870415.1"
                     /translation="ELNCETDFVAKTDDFLALAKELAMQVAASMPKYVVREEVPAEEL
                     QKEREIYLNEAKQSGKPEAVQAKIAEGKLESYYKQVCLMEQPYIREAKKSVKQYVTEV
                     AAKVGENVVVRRFARFQVGE"
BASE COUNT          253 a          551 c          599 g          416 t
ORIGIN      
        1 cggccgttgc cgtccatgat gatggccaag tgccggggga cctgggggcc gagccggtat
       61 tttttcagga aggattcggt ggccatgaaa acgggtacgt cccctcggga aggcggccgc
      121 ccagaagggc cgccgtcaaa attccaggat ctctttttcc ttatgctcgg acagcttctt
      181 gatctcgtcg gtgtattggt cggtcatctt ctggatccgg tcctggccct tcttgacctc
      241 gtcctcgctc agggtcttgt ccttcttgag ctggtccagg tgcttgttgg cgtcctggcg
      301 gatgccccgg atgcccaccc ggccctcctc ggcgtgccgg tgcaccagtt tgacgaattc
      361 cttgcggcgt tcctcggtca gggggggcat gctcaggcgg atgagcttgc cgtcgttggt
      421 gggggtgacg ccgacgtcgc tcttcaagag cgccttctcg atgtcgccca gggcctgggc
      481 gtcccagggc ttgatctcca gcgtgcggga atcggacacc gcgatgttgg ccacctggtt
      541 catgggcacc agggaaccgt aatattcgac cttcacgttc tccaccaggg ccgaggaggc
      601 ccggcccgtg cggatggcgg agaaatcccg cttggtggat tcgatggcct tcttcatgcg
      661 ggcttcggcg tcggcgagga tggattggat gtcggccacg gcgttgctcc ttagcgcacc
      721 agggtcccga tgggttcgcc ccagatgacg cgcttgatgt tttccttctg ctgcaggctg
      781 aagaccacga tgggcagctt gttctccatg cagagggtga gcgcggtggt atccatgacc
      841 ttgaggcgcc ggttgatggc gtcgatgtag gtaagggtct tgaacttctt ggccttgggg
      901 tccttcttgg ggtcggcgct gtagatgccg tccacgttgg tggccttcag gatggcgtcc
      961 gcgccgatct cgatggcgcg cagggccgcg gccgtgtcgg tggtgaaata ggggttgccg
     1021 gtgccgccgg cgaagatcac gatgcggtgc ttttccaggt ggcgcatggc gcggcggcgg
     1081 atgaagggct cggccacctt ggacatctcg atggcgctct gcacccgggt ataggcgccc
     1141 actttttcca gggcgttctg cagggccagg gcgttgatga gggtggccag catgcccatg
     1201 tagtcgctgg agacccggtc catgcccatt ttttcgccgt cggcgccgcg gtagatgttg
     1261 ccgccgccga tgaccagggc cacctgcacg cccttttggt gcacgccgtg gaccgcctgg
     1321 gcgatggcct gcaccacccc ggcgtcgatg ccgtatccct tggagccggc cagggcctcc
     1381 cccgagatct tcaacaatac cttcttatag agcggcttct tggcggaggg ggaagaagcc
     1441 gccatggctc actcccccac ttggaagcgg gcgaagcgcc ggaccacgac gttctcaccc
     1501 actttggcgg ccacttcggt gacgtactgt ttgacgcttt tcttggcctc gcggatgtag
     1561 ggctgttcca tcaggcagac ctgcttgtag tagctttcca gcttgccttc ggcgatcttg
     1621 gcctgcacgg cctcgggctt cccggactgc ttggcctcgt tcaggtagat ctcccgttcc
     1681 ttttgcagct cctcggcggg cacttcctcg cgtaccacgt acttgggcat ggaggcggcc
     1741 acctgcatgg ccagctcctt ggcgagcgcc aagaaatcgt ccgtcttggc cacgaagtcg
     1801 gtctcgcagt tgagctcca
//
LOCUS       DASTJJ010000651         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1190165, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000651 DASTJJ010000000
VERSION     DASTJJ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190165"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFR02_07975"
     CDS             <1..227
                     /locus_tag="VFR02_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870416.1"
                     /translation="NVTATLKGNTQVTASYPGFPDGSQTIPMNYINVQAPTDFEAHYL
                     AGLDIGSVWSIVGEANGPSLSLNTSFRAEF"
     gene            254..469
                     /locus_tag="VFR02_07980"
     CDS             254..469
                     /locus_tag="VFR02_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9870417.1"
                     /translation="MASVKKLNRNSYVDQAECTGCEACVITAPKVFRMTPTGLSEAFD
                     PQGSPESLIQEAMDGCPVSCIHWGTPA"
     gene            466..939
                     /locus_tag="VFR02_07985"
     CDS             466..939
                     /locus_tag="VFR02_07985"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9870418.1"
                     /translation="MSAPQEPMRTGWMEKRQHERIDASIKVAYHLVPKEGLLEVMSQP
                     AYKDSTADHLPELSKKSSVIHAVTQDISVGGLSLVGQDPFPLNSALEVHLYLPAYPSP
                     VTLLTEVMRSTAMAAGVNGQTYRAGLKILAVNRKDVIRIEKFLLEEKLRQKHGRR"
     gene            926..1801
                     /locus_tag="VFR02_07990"
     CDS             926..1801
                     /locus_tag="VFR02_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9870419.1"
                     /translation="MDAADPPLLVLSTEKAAGYRVPGHPEHPQRVLASLRHLKGLLPA
                     AVFEEPSHAPREAIGRVHATALVEAVEREEFLDPDTPGAPGTWACSLLSAGAALRAAG
                     AALSGRRAFSLMRPPGHHATPGAGMGFCYFNSMAVAVQAQVDSGKARRVAVLDLDCHH
                     GNGTEDFCMGKDGLLYVSIHQSPAYPGTGTQSRENCLNYPLPPGTTEGGYFPVLEKAL
                     RAVRGFKPDLVGISMGFDTYERDPLTQFGLKRGDFAALGKQTRALGFPQFALLEGGYH
                     ADLPFLVEEFLGGWA"
     gene            1802..>1981
                     /locus_tag="VFR02_07995"
     CDS             1802..>1981
                     /locus_tag="VFR02_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870420.1"
                     /translation="MVRTWTVDRVNALNIGLMALSAALAFRFPFELFLFSYTVLGPLH
                     YLTEISWLKDRGFYTQ"
BASE COUNT          322 a          691 c          634 g          334 t
ORIGIN      
        1 ggaacgtaac cgcgaccctg aaggggaaca cccaagtgac ggccagctat ccgggtttcc
       61 cggacgggag ccaaaccatt cctatgaatt acatcaatgt ccaggccccc accgacttcg
      121 aggcccacta cctggcgggc ttggacatcg gttcggtctg gtccatcgtg ggggaggcca
      181 acgggccctc cttgtccctc aataccagct tccgcgccga gttttaagcg gccgggcggg
      241 gaagggcgcg tccgtggcca gcgtgaagaa gctcaaccgg aattcctacg tggaccaggc
      301 cgagtgcacg ggctgtgagg cctgcgtgat caccgcgccc aaggtcttcc gcatgacccc
      361 cacgggcctg tccgaggcct tcgaccccca ggggtccccc gagagcctga tccaggaagc
      421 catggacggc tgtccggtga gctgcatcca ttgggggacc cccgcgtgag cgccccccag
      481 gaacccatgc gcacgggttg gatggagaaa cgccagcatg agcgcatcga cgccagcatc
      541 aaggtggcct accacctggt gcccaaggag ggcctgctgg aggtgatgag ccagcccgcc
      601 tacaaggact ccaccgccga ccacctgccc gagctttcca agaaatccag cgtcatccac
      661 gccgtcaccc aggacatctc ggtggggggc ttgagcctgg tgggccagga ccccttcccg
      721 ctgaattcgg ccctggaagt gcacctctac ctgccggcct acccgagccc ggtgaccctc
      781 ttgaccgaag tgatgcgctc caccgccatg gccgcgggcg tcaacggcca gacctaccgg
      841 gcgggcttga agatcctggc ggtcaaccgc aaggacgtca tccgcatcga gaaattcctc
      901 ctggaagaaa aactccgcca gaagcatgga cgccgctgac ccgcccctgc tggtgctctc
      961 caccgagaag gccgccggct accgggtgcc gggccatccc gagcatcccc aacgcgtcct
     1021 ggcgtccctg cgccatctga agggcctgct gcccgccgcc gtcttcgagg aaccctccca
     1081 cgccccccgg gaagcgatcg gccgggtgca tgccacggcg ctggtggagg cggtggagcg
     1141 ggaggagttc ctcgacccgg acaccccggg cgcgccgggc acctgggcct gcagcctctt
     1201 atccgcgggt gcggcgttgc gggccgccgg ggccgcgctc tcgggccgcc gggccttttc
     1261 cctcatgcgg ccgcccgggc accacgccac gcccggcgcc gggatgggct tctgctattt
     1321 caactcgatg gcggtggccg tgcaggccca ggtcgactcg gggaaggccc ggcgcgtcgc
     1381 ggtgctggac ctggactgcc accatggcaa cggcacggag gatttctgca tgggcaagga
     1441 cggcctgctc tacgtctcga tccaccagtc ccccgcttat ccgggcacgg ggacccagtc
     1501 ccgggaaaat tgcctcaatt accccctgcc gccgggcacc accgaaggcg gttatttccc
     1561 cgtgttggag aaggccttgc gggcggtgcg cggcttcaaa ccggacctgg tggggatctc
     1621 catgggtttc gacacctatg agcgggatcc cctgacccag ttcgggttga aacgcgggga
     1681 cttcgccgcg ctggggaagc aaacgcgggc cctgggtttc ccccagttcg ccctgctgga
     1741 gggcggctat cacgccgacc tgcccttctt ggtggaggag ttccttgggg gatgggctta
     1801 gatggtccgg acttggacgg tggaccgggt caatgccctc aacatcggcc tgatggcgct
     1861 ctcggccgcg ctggccttcc ggttcccctt cgagcttttt cttttctcct ataccgtgct
     1921 gggcccgctg cattacctga ccgagatctc ctggctcaag gaccggggct tctacaccca
     1981 g
//
LOCUS       DASTJJ010000652         1837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1801207, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000652 DASTJJ010000000
VERSION     DASTJJ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801207"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFR02_08000"
     CDS             <1..535
                     /locus_tag="VFR02_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870421.1"
                     /translation="ALQGVVHTLTLSTNVIRPDATGGGAPALVAIQVDNGLSIPAWDG
                     DNDQGVQVQSGTYYVAVHTVDGHGGEATLTEGIAVMNGPPTAGLVLATPNQLAGPYPN
                     FTFTVAPAWAESVVDVRIYDLAGELITHFSAAPQGGKVSWRAGRVASGLYLAVVTLKT
                     AGGDQLARETLRLVVLQ"
     gene            complement(589..1656)
                     /locus_tag="VFR02_08005"
     CDS             complement(589..1656)
                     /locus_tag="VFR02_08005"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9870422.1"
                     /translation="MNLSDIEKHLGKDAKDLLGYQCKGISKDSLHLPGGDSVDRIFSL
                     SDRNNRVLSSLQWLHSTGRLAGTGYVSILPVDQGIEHSAGASFAKNPVYFDAENIVKL
                     AIEGGCNSVASTLGVLGIVSRKYAHKIPFIVKLNHNELMTYPNKASEFYFGSVKQAYD
                     MGAAGIGATIYFGNDTSQREIEETTRAFQQAHEYGMFTVLWCYLRNKAFKTKDKDFHL
                     AADLTGQANHLGVTIEADIIKQKLPENNGGFMFLNQESNYGKFDKRMYETLIPDHPIE
                     YTRYQVANCYMGRAGLINSGGASGDNDFAEAVKTAVINKRAGGMGLISGRKAFQRPMA
                     EGAKLLHTIQDVYLSKDVTIA"
BASE COUNT          314 a          541 c          631 g          351 t
ORIGIN      
        1 ggccctgcag ggcgtggtgc ataccctgac gctttccacc aacgtcatcc ggcccgacgc
       61 caccgggggc ggcgccccgg cgttggtcgc gatccaggtg gacaacggcc tgtccatccc
      121 ggcttgggac ggggacaacg accagggggt gcaggtgcag agcgggacct attacgtcgc
      181 ggtccacacg gtggacggcc acggcgggga agcgaccctg accgagggga tcgcggtgat
      241 gaacgggccc ccgacggccg gactggtgct ggccaccccc aaccaattgg ccgggcccta
      301 tccgaacttc accttcacgg tggcgccggc ttgggccgaa tcggtggtgg acgtgaggat
      361 ctatgacctg gccggggaat tgatcaccca tttctcggcg gcgccccagg gcggcaaggt
      421 gtcctggcgg gccggccgcg tcgccagcgg cctctacctg gcggtggtca ccctcaagac
      481 cgcgggtggg gaccagttgg cccgggaaac cctgaggctg gtcgtgctcc aataggcaat
      541 aaaaaagccc ccgggcctgg gcccgggggc ttggtcaacc tcgcggcttt aagcgatggt
      601 gacatccttc gacaaataca cgtcctggat ggtgtgcagc agtttggcgc cttccgccat
      661 gggccgctgg aaggccttgc ggcccgagat gagccccatg ccgcccgcgc gcttgttgat
      721 gacggcggtc ttcacggcct cggcgaaatc gttgtcgccc gaggcgccgc ccgagttgat
      781 gaggcccgcg cggcccatgt agcagttggc cacctggtag cgggtgtact cgatggggtg
      841 gtcggggatg agggtctcgt acatgcgctt gtcgaacttg ccgtagttcg actcctggtt
      901 caggaacatg aaaccgccgt tgttctcggg caacttctgc ttgatgatgt cggcctcgat
      961 ggtcacgccc aggtggttgg cctggccggt caggtcggcc gccaagtgga agtccttgtc
     1021 cttggtcttg aaggccttgt tgcgcaggta gcaccagagc acggtgaaca tgccgtattc
     1081 gtgggcctgc tggaaggccc gggtggtctc ctcgatctcg cgctgggagg tgtcgttgcc
     1141 gaagtagatg gtggcgccga tgcccgccgc gcccatgtca taggcctgct tgaccgagcc
     1201 gaagtagaac tcggaggcct tgttggggta ggtcatcagc tcgttgtggt tgagcttcac
     1261 gatgaagggg atcttgtggg cgtatttgcg cgatacgatg cccaagacgc ccaaggtgga
     1321 ggccaccgag ttgcagccgc cctcgatggc cagcttcacg atgttctcgg cgtcgaagta
     1381 gacggggttc ttggcgaagg aggccccggc ggaatgctcg atgccctggt ccaccggcag
     1441 gatggacaca taacccgtgc ccgccaggcg gccggtggaa tgcagccact ggaggctgga
     1501 gagcacccgg ttgttgcggt cgctcaggga gaagatgcgg tcgaccgagt cgccgccggg
     1561 aagatgcagc gaatccttgg agatgccctt gcactggtag cccagcaggt ccttggcgtc
     1621 cttgcccagg tgtttttcga tgtccgagag gttcatgttt ccgtcctttg cgccttcagg
     1681 gtatggccgt tttggccaat aaacaggccc aaaacgaggc cctagtaata cagaataaag
     1741 aaggaattgg caagcaaaag aaacccgcta tttccaggcc gaccggaact cctcttcctg
     1801 gaagccgacg gtggcctggg tcccgtcgaa ggtcagg
//
LOCUS       DASTJJ010000653         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_457500, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000653 DASTJJ010000000
VERSION     DASTJJ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457500"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFR02_08010"
     CDS             <1..878
                     /locus_tag="VFR02_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9870423.1"
                     /translation="TREDFEKMVAPLVERSLEPCRKALRDANLTPNDLAEVLLVGGMT
                     RMPMVREAVKRFFNKEPNTKVNPDEAVAMGAAIQAGILNGKVRNALLLDVTPLSLGVE
                     TLGGSFSPIIPRNTTVPTKMSKLYATVKDMQPSVNIKVLQGESKIAAQNKVLGFFSFV
                     GLPLAPAGSVQVEVTFAIDSNGIVSVTAKNVATGQTQQMKVSATSGLSKDELEKLVQG
                     AKGRVISSAPAEEKPQAAEPAAKTAGSGGFSLLHSLTGSHKKAEEAAPKPPEGEFLTG
                     DMIDSLFKDDDEGGQ"
     gene            886..>1096
                     /locus_tag="VFR02_08015"
     CDS             886..>1096
                     /locus_tag="VFR02_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET9870424.1"
                     /translation="MNSVAIFGGTFNPIHYGHLAIAEEVRTKFNLDKVIFLPAYLPPH
                     KTGDDLADARHRFLMAHLATVSNPCF"
BASE COUNT          221 a          357 c          347 g          171 t
ORIGIN      
        1 tcacccggga agatttcgag aagatggtgg cccccctggt ggaacggtcc ctggaaccct
       61 gccgcaaggc cctccgggac gccaacctga cgcccaatga cctggccgag gtgctgttgg
      121 tgggcggcat gacccgcatg cccatggtgc gggaggcggt caagcggttc ttcaacaagg
      181 aacccaacac caaggtcaat cccgacgagg cggtggccat gggcgcggcc atccaggccg
      241 gcatcctcaa cggcaaggtg cgcaacgccc tcctgttgga cgtcaccccg ctgtcgttgg
      301 gtgtggagac cctggggggg agcttcagcc ccatcatccc ccgcaacacc acggtcccca
      361 ccaaaatgag caaattgtac gccaccgtga aggacatgca gccctcggtg aacatcaagg
      421 tgctgcaggg ggaatcgaag atcgcggcgc agaacaaggt cctgggcttc ttctccttcg
      481 tggggctgcc cctggccccc gcgggctccg tgcaggtgga ggtcacgttc gccatcgatt
      541 ccaacggcat cgtgagcgtg acggccaaga acgtggccac gggccagacc cagcagatga
      601 aggtctcggc caccagcggt ttgagcaagg acgagctgga aaagctggtc cagggggcca
      661 aaggcagggt catttccagc gccccggccg aggagaagcc ccaggccgcc gagccggcgg
      721 ccaaaacggc cggatcaggc ggattttcgt tgttgcattc cctcacgggt tcgcacaaaa
      781 aggccgagga ggccgccccc aagccgccgg agggcgagtt cctcacgggg gacatgatcg
      841 acagtctgtt caaggacgac gacgagggag ggcagtgatc cccttatgaa ttcggtggcc
      901 attttcggcg gaaccttcaa ccccatccac tacggccact tggccatcgc ggaggaagtg
      961 cgcaccaaat tcaacctgga caaggtcatt ttcctgccgg cctacctgcc gccccacaag
     1021 acgggcgacg acctggcgga cgcccggcac cgtttcctga tggcgcacct ggccacggtc
     1081 agcaacccct gctttg
//
LOCUS       DASTJJ010000654         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1068902, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000654 DASTJJ010000000
VERSION     DASTJJ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068902"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="radA"
                     /locus_tag="VFR02_08020"
     CDS             <1..975
                     /gene="radA"
                     /locus_tag="VFR02_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET9870425.1"
                     /translation="KLRADRLGIRSNNLYILPETDVSALLAHIEKHRPRLTVIDSIQT
                     LFLSDIASAPGSVSQVRESAARLTYLAKREKLPMFLIGHVTKEGNIAGPMTLEHMVDA
                     VLYFEGDLQRHYRLLRAVKNRFGSTQEIGIFEMREDGLKEVADASKAFLETHYTHHSG
                     SAITVSLEGTRPLMLEVQALVSPTNFGMPQRRSSGMDPNRLALLLAILEKRQGMALST
                     QDVFLNVAGGIKVEEPSLDLSVALAAASSFREIPLDPGTVVLGELGLSGEVRAVPQIE
                     PRLAEAARLGFKQAVIPFGNARSAAAHPSLEVLGVKTLSEAIGLLLPR"
     gene            972..1655
                     /locus_tag="VFR02_08025"
     CDS             972..1655
                     /locus_tag="VFR02_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9870426.1"
                     /translation="MREREVLARLRALKNARNIRGMARFGIQGKKVLGIPKPRLDGLA
                     KKIGRDQALSLKLWGSGYHEARILAGLVGSAAEVTPAQMERWVGDFDSWDLCDQVCGH
                     LFDRTPFAASKAFQWSRRKREFEKRAGFVLMAGLAVHDKRAPDRLFLSFLPVVRREAR
                     DGRNFVKKAVNWALRQIGKRNPRLRRAALAEAARIQQQGSASARWIAADAIRELKNPK
                     TPVRKLPLA"
     gene            1700..2398
                     /gene="ispD"
                     /locus_tag="VFR02_08030"
     CDS             1700..2398
                     /gene="ispD"
                     /locus_tag="VFR02_08030"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET9870427.1"
                     /translation="MSTPLFRPWAAILVAAGRSTRLKSPVPKPFLKLDRRRTLLDCCL
                     DSFKKVKGLACVVVVTQPDHLEKAIDHLYRRGLAGIACVGGKEREDSVLQGLLVVPKG
                     IRTVLVHDAARPFVTPRLVQSVLEEALRSGAAIPGLPVKDTLKETLRGRVVRTLDRDS
                     VRAAQTPQGFRADLLRRAFLKAADRRSKFTDDAAVAEAAGFPVRLVEGDPLNFKVTTP
                     DDLARARALARGRI"
     gene            2401..2880
                     /gene="ispF"
                     /locus_tag="VFR02_08035"
     CDS             2401..2880
                     /gene="ispF"
                     /locus_tag="VFR02_08035"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET9870428.1"
                     /translation="MEFRVGTGFDAHRFEAGRPLVLGGVTLPHDKGLLGHSDADALCH
                     AVGDAVLGAAALGDLGKFFPDSDPKWKGADSLKLLAECARLAGEKGWRVVNVDATLVC
                     QRPKLSAYLPEMAKRLAEALGLGPDAVSVKAKTTEGMGFEGREEGLSAQAVVLLTRK"
     gene            2987..>3532
                     /locus_tag="VFR02_08040"
     CDS             2987..>3532
                     /locus_tag="VFR02_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET9870429.1"
                     /translation="MEKVRVRFAPSPTGDLHLGNVRTALYNWLYAKHTGGAFILRVED
                     TDKVRSTEKSFEGLLEIMKWLGLDWTEGPGVGGPHAPYRQSERTGIYAAHLEKLKAGG
                     WVYPCYCSEEELELERKRQLGRGQMPKYGGKCLKLTEEERRKYDAEGRPSVLRFQVPA
                     GAVLVQDEVRGEVVFPDDQIGD"
BASE COUNT          608 a         1113 c         1214 g          597 t
ORIGIN      
        1 aagctgcgcg ccgaccggtt gggcatccgg tccaacaacc tctatatcct gcccgagacc
       61 gatgtgtcgg ccctcttggc ccacatcgag aagcaccggc cgcgcctgac cgtgatcgat
      121 tccattcaga cccttttcct gtccgacatc gcctcggcgc ccggcagcgt gtcccaagtg
      181 cgtgaatccg ccgcgcggct cacctacctg gccaagcgcg aaaaactgcc catgttcctc
      241 atcggccacg tgaccaagga gggcaacatc gccgggccca tgacgctgga acacatggtg
      301 gacgcggtgc tttatttcga gggcgacctc cagcgccact accgcctgct gcgggccgtc
      361 aagaaccggt tcggcagcac ccaggagatc ggcatcttcg agatgcggga ggacgggttg
      421 aaggaggtgg cggacgcctc aaaggccttc ttggagaccc attacaccca ccattcgggt
      481 tcggccatca cggtgagcct ggagggcacc cgccccctca tgctggaagt gcaggccctg
      541 gtcagtccca ccaatttcgg catgccccag cggcggtcct cggggatgga ccccaaccgc
      601 ctggccctct tgttggccat cctggaaaaa cgccaaggca tggcgctttc cacccaggac
      661 gtgttcctga acgtggcggg ggggatcaag gtggaggagc cttccctcga cctgagcgtg
      721 gcgctggcgg ccgcctcttc cttccgggag atccccctgg acccggggac ggtggtgttg
      781 ggggaactgg gcctgtccgg cgaggtgcgg gccgttccgc agatcgagcc ccggttggcg
      841 gaagcggccc ggctggggtt caagcaggcg gtcatcccct tcggaaacgc ccgctcggcg
      901 gcggcccatc cttccctgga ggtcctcggc gtgaagacct tgtccgaggc catcgggctc
      961 ttgctgcccc gatgagggag cgcgaggtcc tggcccggct tcgggcgctc aagaacgccc
     1021 ggaacatccg cggcatggcg cgcttcggca tccaggggaa aaaggtactg gggatcccca
     1081 aaccccggtt ggatggactg gccaaaaaga tcggacggga ccaggccctg tcgctcaagc
     1141 tttggggatc gggatatcac gaagcccgca tcctggccgg cctggtggga tcggcggccg
     1201 aggtcacccc ggcccaaatg gaacgatggg tgggggactt cgacagttgg gacctttgcg
     1261 accaggtttg cgggcatctt ttcgaccgca cgcctttcgc cgcgtccaag gcctttcaat
     1321 ggagccgccg aaagcgggag ttcgagaaac gggccgggtt cgtgctgatg gccgggctgg
     1381 ccgtccatga caaacgcgcg cccgaccgac tgttcctctc gttcctgccg gtcgtgaggc
     1441 gtgaggcccg ggatgggcgc aacttcgtca agaaggcggt caactgggcc ctccgccaga
     1501 tcggcaagcg caatcccagg ctgaggcggg cggccttggc cgaggcggcg cggatccaac
     1561 aacaagggag cgcgtcggcc cgttggatcg cggcggacgc catccgcgag ctgaaaaacc
     1621 caaaaacccc cgtccggaag ctcccgttgg cctaaaaggc cttctggaaa ccgccccccc
     1681 cttttgttaa actgcgccca tgtccacccc cctcttccgt ccctgggccg ccattttggt
     1741 ggcggcgggg aggtccaccc gcttgaagag ccccgttccc aagcctttcc tcaagctgga
     1801 ccgccggcgg accctgctgg attgctgtct ggactccttc aagaaagtga agggtttggc
     1861 ctgcgtggtg gtggtgaccc aacccgatca cctggagaag gccatcgacc atctctaccg
     1921 ccggggcttg gcgggcatcg cctgcgtggg cgggaaggaa agggaggatt cggtcctcca
     1981 ggggctgttg gtggtgccca agggcatccg gacggtgttg gtacatgacg cggcgcggcc
     2041 ttttgtgacc ccccggctcg tccaatcggt gctggaagag gccctccggt cgggggcggc
     2101 catccccggg ctgcccgtca aggacaccct gaaggagacc ctccggggaa gggtggtccg
     2161 caccctggac cgtgattcag tgagggccgc gcagacgccc cagggtttcc gcgcggacct
     2221 gttgcgccgg gccttcttga aggcggcgga ccgccggtcg aaattcaccg acgacgcggc
     2281 ggtggccgag gccgcggggt tccccgtgcg gctggtggaa ggcgacccct tgaatttcaa
     2341 ggtgaccacg ccggatgatc tggcccgcgc ccgtgccctg gcccggggga ggatttgagc
     2401 gtggaattca gggtggggac gggtttcgac gcgcaccgct tcgaggcggg aaggccgctg
     2461 gtcctgggtg gggtgaccct gccccatgac aaggggcttt tgggccattc ggacgccgac
     2521 gccctttgtc acgcggtcgg cgacgccgtc ctgggcgccg cggccctggg cgacctgggc
     2581 aaattctttc cggacagcga tccgaaatgg aagggcgcgg acagcctgaa gctgctggct
     2641 gaatgcgccc ggttggcggg ggagaagggt tggcgcgtcg tcaacgtgga cgccaccctg
     2701 gtctgccaaa ggcccaagct ctccgcctac ctgcccgaga tggccaagcg cctggcggag
     2761 gccctcgggc ttgggccgga tgccgtgagc gtgaaggcca agaccaccga gggaatgggt
     2821 tttgaggggc gcgaggaagg cttgtccgcc caggcggtgg tgctcttgac ccgaaagtga
     2881 tcttggaccc ccgcttctta aatgatgggg atcttgggtc ccgcctgaaa accgcataga
     2941 accaaaatga cgccccggaa gggcgttcaa tagaaggaag agttcattgg aaaaagtaag
     3001 agtccgattc gcccccagcc ccacggggga cctgcacctg ggcaacgtgc gcaccgccct
     3061 ttataactgg ctttacgcca agcatacggg cggggccttc atcctgcggg tggaggacac
     3121 ggacaaggta aggtccaccg aaaagtcctt cgagggcctt ttggagatca tgaaatggct
     3181 gggcttggac tggaccgagg gcccgggcgt gggcggtccc cacgcgcctt accggcagtc
     3241 cgaacgcacc gggatctacg ccgcccacct ggaaaagctc aaggcggggg gctgggtcta
     3301 tccctgctat tgcagcgagg aagagctgga gctggagcgc aagcgtcagt tgggccgggg
     3361 ccaaatgccc aagtacggcg gcaagtgcct gaagctgacc gaggaagagc gccggaagta
     3421 cgacgccgag ggccgccctt cggtgttgcg cttccaggtg cccgccgggg ccgtcctggt
     3481 gcaggatgag gtgcggggtg aagtggtctt tccggacgac cagatcggcg at
//
LOCUS       DASTJJ010000655         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_823476, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000655 DASTJJ010000000
VERSION     DASTJJ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823476"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..699)
                     /locus_tag="VFR02_08045"
     CDS             complement(118..699)
                     /locus_tag="VFR02_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870430.1"
                     /translation="MDLHKVAVKFYVEKDDHVPLSVFIPIFHRFIQEDKLEGLLIDVA
                     QYTHVHQGPGVLLIAHEANYSLDETLGHRGLLYTQKRPAEKGGLDHLKTAFRRALAAA
                     DLLEKQPELAGKLTFATNHFQVFVNDRLAAPHNAESQEDLENALKTLFDGLYEGAATV
                     LMPDMNRQHRAGFEVHVDKAFSREELLKRLAAN"
     gene            complement(713..>1762)
                     /locus_tag="VFR02_08050"
     CDS             complement(713..>1762)
                     /locus_tag="VFR02_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET9870431.1"
                     /translation="LVLRWIPEAALPSVFEALAAVGLSASGANTITDIASCPGTDTCK
                     LGISASRGLAREIISQFEAKAATLPEEIKKLNIKVSGCPNSCSQHHLSDLGFYGVSRK
                     SGSYAVPHFQLVLGGQRKNNGAAYGLAVAAVPSKRIPEAIERLTQKYSKERQKDESFQ
                     AFVHRLGKVEFKTLLQDLTVIPDHDFDPELYTDWADPREYTIGDIGKGECAGEVVAPY
                     EFALTAAESKVFEAQVRLEKGDLAGAVDLAYQSFIHSGEAVLRTRDREYTGKADQTVE
                     DFKKFFFDTGDFVKHVNNTQFAAYLFKAHDNRLPNPNQDEAHHRVEEAQLFLEAVHSY
                     IANLPAAAIAPAPAK"
BASE COUNT          267 a          534 c          607 g          354 t
ORIGIN      
        1 acctgtttac cgcgaagacg ctaagacgcc aagaacagcg aggcattgaa ctcgtgaggc
       61 ttttaaggat tttcctcgcg gcttggcgtc tttgcggtga ataggtttac gtttggatca
      121 gttcgcggcg agacgcttta agagttcttc gcggctgaag gccttgtcca cgtgcacctc
      181 gaaacccgcg cggtgctggc ggttcatgtc gggcatcagc acggtggcgg ccccctcgta
      241 gaggccgtcg aagagggtct tgagggcgtt ctccaggtcc tcctggctct cggcgttgtg
      301 cggcgcggcc aggcggtcgt tgacgaagac ctggaagtgg ttggtggcga aggtcagctt
      361 gcccgccaat tcgggttgct tttccaaaag gtcggcggcc gccagggccc ggcggaaggc
      421 ggtcttcagg tggtccaacc cgcccttctc ggcgggccgc ttctgggtgt agagcaggcc
      481 ccggtggccc agggtctcat ccaggctgta gttggcctcg tgggcgatca acagcacgcc
      541 cggcccctgg tgcacgtggg tgtactgggc cacgtcgatc aagagcccct ccagcttgtc
      601 ctcctggatg aagcggtgga agatggggat gaagacgctc aagggcacgt ggtcgtcctt
      661 ctccacgtag aacttcacgg cgactttgtg caggtccatg ggtctcctcg ggttatttcg
      721 ccggcgccgg cgcgatggcg gccgccggca ggttggcgat gtagctgtgg acggcctcca
      781 ggaacaactg ggcctcctcc acccggtggt gcgcctcgtc ctggttgggg ttgggcaacc
      841 ggttgtcgtg ggccttgaac aggtaggcgg cgaactgggt gttgttcacg tgcttgacga
      901 agtcccctgt gtcgaagaag aacttcttga agtcctccac ggtctggtcg gccttgcccg
      961 tgtattcccg gtcccgggtg cgcagcaccg cctcgcccga gtggatgaag gactggtagg
     1021 ccaggtccac cgcgcccgcc aggtcgccct tttccagccg cacctgggcc tcgaacacct
     1081 tgctctcggc cgcggtcaac gcgaattcat agggcgccac cacttccccg gcgcattcgc
     1141 ccttgccgat gtcgccgatg gtgtattcgc gcgggtcggc ccagtcggta tagagctcgg
     1201 ggtcgaaatc gtggtccggg atgaccgtca ggtcctgcag cagggtcttg aactccacct
     1261 tgcccagccg gtgcacgaag gcctggaagc tctcgtcctt ttggcgttcc ttggaatact
     1321 tctgggtgag gcgctcgatg gcctccggga tgcgcttgga gggcacggcc gccacggcca
     1381 ggccgtaggc cgccccgttg ttcttgcgct ggccgcccag gaccaactgg aaatgcggca
     1441 ccgcgtagga gcccgacttg cgcgatacgc cgtagaagcc caggtcggac aggtggtgct
     1501 ggctgcagga attggggcag ccgctcacct tgatgttgag cttcttgatc tcctcgggca
     1561 gcgtggcggc cttggcctcg aactgggaga tgatttcccg ggccagcccg cgggaggccg
     1621 agatgcccag cttgcaggtg tccgtgcccg ggcaggaagc gatgtccgtg atggtgttgg
     1681 cgccggaggc gctcaagccg acggccgcca gggcctcgaa gaccgaaggc agggccgcct
     1741 cggggatcca gcgcagcacc ag
//
LOCUS       DASTJJ010000656         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_336371, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000656 DASTJJ010000000
VERSION     DASTJJ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336371"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFR02_08055"
     CDS             complement(<1..394)
                     /locus_tag="VFR02_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9870432.1"
                     /translation="MASFPVTRVLTTWFVRLYFRIQVRGESFIPAEGPFVVVANHASF
                     LDPFFIGYASIRRQVGFMAKEELFRVPLFGPLIRRYDAFPVRRGQHDLEAVRRFHDFL
                     HQNKPLLIFPEGTRTLDGELQKAKKGVGL"
     gene            complement(411..1532)
                     /locus_tag="VFR02_08060"
     CDS             complement(411..1532)
                     /locus_tag="VFR02_08060"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9870433.1"
                     /translation="MPLPTAKKTKKQKFLQIGKVTLRAKAQDTHVAASQILITPKKVK
                     AKLPGTKKAYETVLKGRKEIEAILDHKDRRLFVVVGPCSIHDPQAAFDYATRLKKLAG
                     MVRSTMLLVMRVYFEKPRTTIGWKGLINDPDMNDSFHIEKGILLARRLLLKFNEMGLP
                     TSTEALDPIIPQYIADLISWSAIGARTTESQTHREMASGLSMPVGFKNATDGSIQVAL
                     NGIKAARTSHHFLGIDQDGKTAVFRTTGNPYAHIVLRGGHSPNYDAASILACEKGLAE
                     AGLPLNIVVDCSHGNSNKDYRMQPAVFDKVLDQVVAGNRSLVGYMVESNIQEGNQPLA
                     RDPAKLKYGVSVTDACINWETTEELILKAHGRLRKAWGR"
     gene            complement(1621..>1995)
                     /locus_tag="VFR02_08065"
     CDS             complement(1621..>1995)
                     /locus_tag="VFR02_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9870434.1"
                     /translation="LPYAWFADALIWLERGVIGVAFLSALYHNLWKLGTTYKLTDHDL
                     QVSQWFPIRMRQSVPYGAVRRLGSKQGLLGALLDYGHVEVDTGGSVPLVLVNCPRPQK
                     FVETLQHKVEEVLQPQLLQNRS"
BASE COUNT          326 a          591 c          696 g          382 t
ORIGIN      
        1 gaagtcccac ccccttcttg gccttctgca attccccgtc cagggtgcgg gtgccctcgg
       61 gaaagatcaa caacggcttg ttctggtgca ggaagtcgtg gaaacggcgc accgcctcca
      121 ggtcatgctg gccgcggcgc acgggaaagg cgtcatagcg gcggatgagg gggccgaaca
      181 ggggcacgcg gaacagctct tccttggcca tgaatcccac ctgccggcgg atgctggcgt
      241 agccgatgaa gaagggatcc aggaagctgg cgtggttggc cacgaccacg aaggggcctt
      301 ccgccgggat gaaggattcg ccccggacct ggatgcggaa ataaaggcgg acgaaccagg
      361 tggtgaggac gcgggtgacg ggaaaggagg ccatcacggg cgtccggggc tcagcggccc
      421 caggccttcc gcagcctgcc gtgggccttg aggatcagtt cctcggtggt ctcccagttg
      481 atgcaggcgt cggtgacgga cacgccgtac ttgagcttgg cggggtcccg cgccagcggc
      541 tggttgccct cctggatgtt gctttccacc atgtagccca ccagggagcg gttgccggcc
      601 accacttggt ccaacacctt gtcgaagacc gccggctgca tccggtagtc cttgttcgag
      661 ttgccgtggc tgcagtccac cacgatgttc aggggcagtc cggcctccgc caggcccttc
      721 tcgcaggcca ggatggacgc ggcgtcgtag ttggggctgt gcccgccccg cagcacgatg
      781 tgggcatagg ggttgcccgt ggtgcggaag accgccgtct tgccgtcctg gtcgatgccc
      841 aggaaatggt gcgaggtgcg ggccgccttg atgccgttca gcgccacctg gatgctgccg
      901 tcggtggcgt tcttgaagcc caccggcatg gaaaggccgc tggccatctc ccggtgggtc
      961 tgggattcgg tggtgcgcgc cccgatggcc gaccaactga tgaggtcggc gatgtactgc
     1021 gggatgatgg ggtccagcgc ctccgtggag gtgggcaggc ccatctcgtt gaacttcaac
     1081 agcaggcgcc gggccaagag gatgcccttc tcgatgtgga aggaatcgtt catgtccggg
     1141 tcgttgatca ggcccttcca gccgatggtg gtgcggggct tttcgaaata gacccgcatg
     1201 accagcagca tggtcgagcg caccatgccc gccagcttct tcaggcgggt ggcgtaatcg
     1261 aaggcggctt gggggtcatg gatggaacag gggcccacca ccacgaagag ccggcggtcc
     1321 ttgtggtcca ggatcgcctc gatctccttg cggcccttga gcacggtctc ataggccttc
     1381 ttggtgccgg gaagcttggc cttcaccttc ttgggcgtga tgaggatctg ggaggcggcc
     1441 acatgggtgt cctgggcttt ggcgcgcagg gtgaccttgc cgatctgcag gaacttttgt
     1501 tttttggttt ttttggccgt cggaagtggc atgcgggcgc cttttcgggt ttagggtggc
     1561 tgaattatag ggattcggcg ggcccgttcc aagcccaaag cgcccgggcc cgcggggcct
     1621 tcaggaacgg ttctggagca gttggggctg cagcacttcc tcgaccttgt gctggagggt
     1681 ttcgacgaac ttttggggac gggggcaatt gaccagcacc aagggcacgc tccccccggt
     1741 gtcgacctcc acatgcccgt aatccaacag ggcccccaac agcccctgct tggatcccag
     1801 gcgccgcacc gcgccatagg ggacgctttg gcgcatccgg atcgggaacc attgggagac
     1861 ctggaggtcg tggtcggtca gcttgtaggt ggtgcccaac ttccagaggt tgtgatacag
     1921 ggcgctcagg aaggccacgc cgatgacgcc ccgctccagc cagatcaggg cgtccgcgaa
     1981 ccaggcatag ggcag
//
LOCUS       DASTJJ010000657         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_823542, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000657 DASTJJ010000000
VERSION     DASTJJ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823542"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1756
                     /locus_tag="VFR02_08070"
     CDS             <1..>1756
                     /locus_tag="VFR02_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9870435.1"
                     /translation="LTLLAASGICVRPPADVPVPWMVGAWSAAFLWLFLLDRPKVFLS
                     PSRALFVLGTTVGLSLLIQSTGADQSPLIFALFVLMGVVGWHGEAKFTFWVAILFSLL
                     EAVALRHQSTQGWVFYLRWAGLLISAFFLVRIVRTRVEKERLGQKLESLKTEAGRLAR
                     AEPSSFNVPKDKLLLEESRLTARVGSVMGLEESLTQQAALFQKALKLHSVAIFLLTTL
                     EDKQVFRLRASATASDSLAPDVTLRPGETLVGLAAKEEKRVLLNQMAPEAARALPYYL
                     RPNPVGSFLAQPLFVKEAAGPGEKRVLAGLAVLDSLSENFFGEHELELVEGFCANLEG
                     AVEHHQSLHFSKTKSRNLHALYEVSKSFSTLLNFDKVLSTALETARDIFPCDSAYLAL
                     AEEDGKNFGVRVWWGPSWTVPRPTQLDPELAAWVLDNKKPIRYTRGQKDRNLAPFARR
                     EGMLGSIQSFLMVPLLVGGDVWGVIRLNSHLRDAYQEYDQDVLSTLANQTAMALQNAV
                     NIRHIENLAQRDGLTGVYNHRYFQEKLEEEIVQASRYNKDLSFLLLDVDHFKKFNDTY
                     GHQEGDRVLRAVADIAQST"
BASE COUNT          304 a          546 c          552 g          354 t
ORIGIN      
        1 cttgaccctc ttggccgcca gcgggatctg cgtccgtccc cccgccgacg tgccggtgcc
       61 ctggatggtc ggggcttggt cggcggcctt cctttggttg ttcctgctgg accgtcccaa
      121 ggtcttttta agccccagtc gggccctttt tgtcctgggc accacggtgg ggctcagcct
      181 tctcatccag tccaccggcg ccgaccagtc cccgctgatc ttcgcgctct tcgtcctgat
      241 gggcgtggtg ggatggcacg gggaggccaa gttcaccttt tgggtggcca tcctcttcag
      301 cctgttggag gcggtggcgt tgcggcatca aagcacccag gggtgggtct tctacctgcg
      361 ctgggcgggg ctgctcattt cggctttttt cctggtccgc atcgtcagga cccgggtgga
      421 aaaggaacga ttgggccaaa agttggaatc cctgaagacc gaggcgggcc gcttggcccg
      481 ggccgaacct tcctccttca acgttcccaa ggacaagttg ctgctggaag agagccgctt
      541 gaccgcccgg gtggggtcgg tcatggggct ggaggagtcc ttgacccaac aggccgccct
      601 tttccagaag gccctcaaac tgcactcggt ggccatcttt ctcctgacca ccttggagga
      661 caagcaggtg ttccgcctgc gggcctccgc caccgcctcc gattcgctgg cccccgacgt
      721 gaccctgcgg ccgggggaaa ccctggtggg gctggccgcg aaggaagaaa aaagggtgtt
      781 gctcaaccaa atggcgcccg aagccgcccg cgcgctgcct tattacctgc gtcccaatcc
      841 ggtgggttcc ttcctggccc agcccctttt cgtcaaggaa gcggcgggtc ccggggaaaa
      901 gcgcgtcttg gccggtctgg cggtgttgga cagcctttct gaaaatttct ttggggaaca
      961 tgagctggag ctggtggagg gattttgcgc gaacctggaa ggggcggtgg agcaccacca
     1021 gtccctgcac ttttcgaaga ccaagagccg gaacctccac gccctctacg aagtgtccaa
     1081 gtccttttcc accctgttga atttcgacaa ggtgctttcc acggccctgg aaacggcccg
     1141 cgacatcttt ccttgcgatt cggcctacct ggccctggcc gaggaggatg ggaaaaattt
     1201 cggcgtgagg gtctggtggg ggccttcctg gaccgtgccg cggccgaccc aattggaccc
     1261 cgaattggcc gcttgggtgc tggacaataa aaaacccatc cgctacaccc gcggccagaa
     1321 ggaccgcaac ctggccccct tcgccaggcg ggaaggcatg ctgggatcca tccagtcctt
     1381 cctgatggtg cctttgctgg tggggggcga tgtgtggggc gtgatccgcc tcaacagcca
     1441 tttgcgggac gcctaccagg aatatgacca ggacgtgctg agcaccctgg ccaaccaaac
     1501 cgccatggcc ctgcagaacg cggtgaacat ccgccacatc gagaacctgg cccaacggga
     1561 cggcttgacc ggcgtctata accaccggta tttccaggag aagctggagg aggagatcgt
     1621 ccaggcgagc cgttacaaca aggacttgag tttcctgctc ttggacgtgg accacttcaa
     1681 gaaattcaac gacacctacg ggcatcagga gggggaccgg gtgcttcggg cggtggcgga
     1741 tatcgcccag tccacc
//
LOCUS       DASTJJ010000658         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1559078, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000658 DASTJJ010000000
VERSION     DASTJJ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559078"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFR02_08075"
     CDS             complement(<1..321)
                     /locus_tag="VFR02_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870436.1"
                     /translation="MRNIEEVAAEEELRSRRDQRRFLRRALNAAGLALGLLGWTFFSQ
                     GDLGSGAFWILLSLAAFLVGWGAHRLNAFPWSAWGMALPVAAAVLGLDLLTGLPSFYW
                     GRDPD"
     gene            complement(321..1070)
                     /locus_tag="VFR02_08080"
     CDS             complement(321..1070)
                     /locus_tag="VFR02_08080"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9870437.1"
                     /translation="MIWAVDIGNSRTVSGRLKGLRVLRRRTAPTPPLRGLSAARAWAR
                     GLKRLGGADRVVVSSVVPPVDGPVRRALGEVFGRRPDFVVPGDPGLSIPVRYRRPREV
                     GADRVVNGLAARHLFGAPVIVVDYGTGTTFDVVDAAGAYRGGVIVPGIGMSLKAMHDQ
                     TAKLPLVRFAKVRRTVGRTTADAIRSGIYYGTLGETREILARIRAELGAPRCPAVATG
                     GWCGVFRGSGLFKAIAPDLTLQGLSLFAEEA"
     gene            complement(1067..>2085)
                     /locus_tag="VFR02_08085"
     CDS             complement(1067..>2085)
                     /locus_tag="VFR02_08085"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET9870438.1"
                     /translation="PSHLRILSSLSRRPRRPVALGRLAREAGLTAAGLDGELARMEGQ
                     GYGLERGPRGIRLVAEPDRLYPERLMRGLETDRLGRELQSFQTIGSTNDRALQLGEKG
                     AADGTVLVADRQTRGRGRRGRAWALAEDKGLAFSVILRPALPAAEAPGLTLAAAVAVA
                     RTLEEWKVRPRIKWPNDLFLGGRKVCGILTETRGREDKINFAVVGVGVNLNQAAGDFP
                     PELRATATSLFRHRGIRVDRAAFFRRLLLHLEKAEGWVRRRQFHRVLAEWRRRSFLNG
                     RQVRIREADRTLYGQVQGVDETGALLVRNDWGMVERVLAGDVELLRLSRRRPRPGIRK
                     RGGA"
BASE COUNT          320 a          820 c          694 g          251 t
ORIGIN      
        1 gtcggggtcc cgcccccaat agaaggaagg caggcccgtc agcaggtcca accccaacac
       61 cgccgcggcc acgggcaggg ccattcccca ggcggaccaa gggaaagcgt tcaggcggtg
      121 ggcgccccat cccaccagga aggccgcgag ggaaaggagg atccagaagg cgcccgatcc
      181 caggtccccc tgggagaaga acgtccaccc gagcaggccc agggccaggc ccgcggcgtt
      241 cagcgcccgg cgcaggaacc ggcgttggtc ccggcgcgag cgcagttcct cttcggcggc
      301 gacttcctcg atattgcgca tcacgcctcc tccgcgaaaa gtgacaggcc ctgcagggtc
      361 aggtcggggg cgatggcctt gaacaacccc gacccccgga agacgccgca ccagccgccc
      421 gtggccaccg ccgggcagcg gggcgccccc aactcggccc ggatgcgggc caggatctcc
      481 cgggtctcgc ccagggtgcc gtaatagatg cccgaccgga tggcgtccgc ggtggtgcgg
      541 cccaccgtgc gccgcacctt ggcgaaacgc accaggggca gtttggcggt ctggtcgtgc
      601 atggccttga gcgacatgcc gatgccgggc acgatcacgc ccccccggta ggccccggcg
      661 gcgtccacca cgtcgaaggt ggtgcccgtg ccgtagtcca ccacgatgac cggggcgccg
      721 aacaaatgcc gggccgccag gccgttcacc acgcggtccg cgcccacttc ccggggacgg
      781 cggtaccgca ccgggatgga aagccccggg tccccgggga ccacgaagtc gggccggcgg
      841 ccgaagacct cgcccagggc ccggcgcacc gggccgtcca ccgggggcac cacactggac
      901 accaccaccc ggtccgcccc gcccaggcgc ttcaggcccc gggcccaggc ccgggccgcg
      961 gacaaccccc gcaagggcgg cgtgggggcc gtccgacggc gcaatacccg caggcccttc
     1021 aagcgtcccg aaaccgtccg gctgttgccg atgtccaccg cccagatcat gcgccgcccc
     1081 ttttccttat ccccgggcgc gggcggcgcc gggaaagccg aagcagttcc acgtcccccg
     1141 ccagcacccg ttccaccatg ccccagtcgt tgcgcaccaa gagcgcgccc gtctcatcca
     1201 ccccttgcac ctgcccgtaa agcgtccggt cggcttcccg gatccgcacc tgccgcccgt
     1261 tcaggaagga ccggcggcgc cattcggcca ggacccggtg gaactggcgg cggcgtaccc
     1321 agccttcggc cttctccagg tgcaggagca ggcgccggaa aaaggccgcc cggtccaccc
     1381 ggatgccccg gtgccggaac aggctggtgg ccgtggcgcg cagttcgggg ggaaaatccc
     1441 ccgccgcctg gttcaggttc acgcccaccc ccaccaccgc gaagtttatt ttatcctccc
     1501 ggccccgggt ctcggtcaag atgccgcaga ccttgcgccc ccccaggaaa aggtcgttgg
     1561 gccacttgat gcgcggccgg accttccatt cttccagggt ccgggccacc gccaccgccg
     1621 ccgccagggt caggccgggc gcttcggccg ccggcaaggc tgggcgcagg atcaccgaaa
     1681 aggccaggcc cttgtcctcg gccagggccc aggcccgccc ccgccggccc cgcccccggg
     1741 tttggcggtc ggccaccagc accgtgccgt ccgccgcccc cttttccccc agctgcaggg
     1801 cccggtcgtt ggtggaaccg atggtctgga aggactgcaa ttcccggccc agccggtccg
     1861 tttccaaacc ccgcatcagg cgctcgggat aaagccggtc gggttcggcg accaagcgga
     1921 tgccccgggg cccccgctcc aagccgtagc cctggccctc catgcgggcc aattccccgt
     1981 ccaacccggc ggccgtcagc cccgcctccc gggccaggcg gcccagggcg acggggcggc
     2041 ggggccggcg cgacaggctg gacaggatcc tgagatggga cggcg
//
LOCUS       DASTJJ010000659         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_579744, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000659 DASTJJ010000000
VERSION     DASTJJ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579744"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFR02_08090"
     CDS             complement(<1..692)
                     /locus_tag="VFR02_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9870439.1"
                     /translation="MAPGLKPNLAALLGCVLLIGLGEELWSRFIPPYLLALGGTAWMA
                     AAYGTLRDLLDALYSYPGGWLNDRLGGCRALLLFTGLSLAGYVLYLVSPNGWVFLLGA
                     CLALAWTSFSMPALFDIVAKNLASEHRTWGFSLQAILKRVPLLLGPPLGGWLIARWGI
                     LTGFRAGLVVTILLTAGALWLLAAYARPDAPARRDGRSALSLWRAMDGRLKSLLGADI
                     LIRLAEGIPDVFV"
     gene            complement(694..1581)
                     /locus_tag="VFR02_08095"
     CDS             complement(694..1581)
                     /locus_tag="VFR02_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HET9870440.1"
                     /translation="MPRAIFSLLLACLAAGAVAAAELPAPWKAQEEFWGAPGWLQDGV
                     WAIGFPRSDLNVMVEGVPLESDLGLTSRLWFKPLTRGLEVRGRLVLLDQECRRVEERL
                     LRDGFRVEGFGNFLLGEAPAVKQLEFSGKGLASVLNPELREVLRLTGTHPVPVSFPTP
                     GPGAADGWEALQTELGLRGWVQGRVLWILSPPFTPVSDPAPGYCSLRLQRDGPTLWVV
                     GENWLAPSQSRGLLALLGRARLTLTAEETAGDAVRVRWWGRGQQEDLAPVLSRVLALG
                     AAAQGPTPTPAASPDPEGF"
BASE COUNT          334 a          616 c          582 g          206 t
ORIGIN      
        1 acgaagacgt ccgggatgcc ctcggccagc cggatgagga tgtccgcccc cagcagggac
       61 ttcaggcggc cgtccatggc cctccacagg gacagggccg aacgaccgtc ccgccgggcg
      121 ggcgcgtcgg gacgggcata ggccgccaaa agccaaagcg cccccgccgt caaaaggatc
      181 gtcaccacca ggcccgcccg gaagccggtc aggatgcccc accgcgcgat gagccatcct
      241 cccaggggcg ggcccaacag caggggcacg cgcttcagga tggcctgcag gctgaagccc
      301 caggtccggt gctcggaggc caggttcttg gccacgatgt cgaagagcgc gggcatggaa
      361 aaactggtcc aggccagggc caggcaggcg cccaacagga agacccatcc gttggggctg
      421 accaggtaaa ggacataccc cgcgagggaa aggccggtga aaagcagcag cgcccggcac
      481 ccgcccagcc ggtcgttcaa ccaaccgccc ggataggaat aaagcgcgtc cagcaggtcc
      541 cgcagggtgc cgtaggcggc ggccatccaa gccgtgcccc ccagggccaa taaatagggg
      601 gggatgaacc gggaccaaag ctcttcgccc aggccgatga gcaacacgca tcccagcaag
      661 gccgccaggt tgggcttcag gcccggggcc acgttaaaag ccctcggggt cgggggaagc
      721 cgccggcgtg ggggtgggcc cttgggcggc cgcgcccagg gccagcaccc ggctcaagac
      781 cggcgccagg tcttcctgct ggccccggcc ccaccagcgc acccggaccg cgtcgccggc
      841 ggtctcctcg gcggtgagcg tgaggcgggc gcgccccaaa agtgccaaca ggccccggct
      901 ttgggaaggc gccagccagt tctcgcccac cacccaaagg gtgggcccgt cccgttggag
      961 ccgcagcgaa caatagcccg gcgccgggtc cgacaccggc gtgaagggcg gggaaaggat
     1021 ccaaagcacc cgtccctgca cccagcccct caggcccaat tcggtctgca gggcttccca
     1081 cccgtcggcc gcacccggcc cgggcgtggg gaaggagacc ggcaccgggt gggttcccgt
     1141 gaggcgcaac acctcgcgca gttcggggtt gaggaccgac gccaggccct tgccggagaa
     1201 ctccagttgc ttcaccgcgg gggcctcccc caacaggaaa ttgccgaagc cttccacccg
     1261 gaacccgtcg cgcagcaggc gctcctccac ccgccggcat tcctggtcca gcagcaccaa
     1321 gcggccccgc acttccaggc cgcgggtcag gggtttgaac cagaggcggg aggtcaggcc
     1381 caggtcgctt tccaggggca cgccttccac catcacgttc aggtccgacc gggggaagcc
     1441 gatggcccag accccgtcct gcagccagcc cggcgcgccc caaaattcct cctgggcctt
     1501 ccagggcgcg ggcagttcgg cggccgccac cgcaccggcg gcgaggcatg ccaagaggag
     1561 tgaaaaaatg gcgcgcggca aggggttcct ttcggttcca agataggggc cttcccggtt
     1621 aagataacat gccaccggtc ccagggccat ttgcgcgtcg aacggtcccg ggccggcccc
     1681 atcctgctaa aaaaatcgga atcatgcatt tcgcaaagga aggataatag gaacactg
//
LOCUS       DASTJJ010000660         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_458721, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000660 DASTJJ010000000
VERSION     DASTJJ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458721"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..924)
                     /locus_tag="VFR02_08100"
     CDS             complement(352..924)
                     /locus_tag="VFR02_08100"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9870441.1"
                     /translation="MNLVLFGPPGAGKGTQSKILTQTRGLPQLSTGDMLRAAIAAGTP
                     LGLASKALMDKGALVPDETVIGIIAERYDQKDCAQGAVFDGFPRTIAQAEALDRMLAA
                     RARKIDLVLELKVDDAVLLKRVEGRVAEGGPLRADDTPETLKKRLQVYYQATAPVLSY
                     YRGQGKVTTVDGMAPIPEVTRQIAGILDRL"
     gene            complement(921..>1174)
                     /locus_tag="VFR02_08105"
     CDS             complement(921..>1174)
                     /locus_tag="VFR02_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9870442.1"
                     /translation="KSAQFIDYTLTRITVVGALYLAFVCLIPEFMFYKYNLSFSLGGT
                     SILIVVSVTMDTVAQIQSHLVAQQYEGLLKKSKLRGARR"
BASE COUNT          186 a          405 c          358 g          225 t
ORIGIN      
        1 acacccggcc gctcgcccaa gctcgcggcg ttggcttagc ttttcttggc gagcaagcga
       61 gcctagaaaa gcttgcccct cttcgagcgg agcgagggag gcggccgctc aaaatggtcc
      121 accggaccat ttttgcccca aagggtcgcg gctcacccct ccggccttcg cagctttgcc
      181 cttggcgctc tcggcttacg cctaagcgcc tgcgggcggt ggctcacggc cacctccccc
      241 gcccgcgtgc tggcgcacgc gcgggggagg cgggcctggg ccaagatatc tccagccctt
      301 gttgcagctt ggttcgaaac cctgttaccg ccaggacgcg ttggacgcgc cctacagcct
      361 atccagaatc ccggcgattt gccttgtgac ctcggggatg ggcgccatgc cgtccaccgt
      421 cgtgaccttc ccctgccccc ggtagtagct gaggaccggg gcggtcgcct ggtaatagac
      481 ctgaagccgc ttcttgaggg tctcaggcgt gtcatcggca cgcagcggcc cgccttccgc
      541 cacccggcct tccacccgct tcaacagcac ggcatcgtcc accttgagct ccagcaccag
      601 gtcgatcttc ctcgcccttg ccgccagcat ccggtccagc gcttcggcct gggcgatggt
      661 gcgggggaag ccgtcgaaca ccgcgccttg ggcgcagtcc ttctggtcat agcgttcggc
      721 aatgatcccg atcacggttt cgtccggaac cagcgcgccc ttgtccatca gggccttgct
      781 ggccagcccc aggggcgtgc ccgccgcgat cgccgcccgc aacatgtccc cggtcgagag
      841 ctggggcagg ccgcgggttt gcgtgagaat cttggattgg gtgcccttgc ccgcccccgg
      901 gggcccgaac agaaccaggt tcacctgcgc gctcctcgca acttggattt cttcagaagg
      961 ccttcgtact gctgcgccac cagatggctc tggatttgcg ccaccgtgtc catggtcacg
     1021 ctgaccacga tcaggattga agtgccgccc agcgagaagc tgagattgta cttgtagaac
     1081 atgaattcgg gaatcaggca gacgaaggcc agatagagcg ccccgaccac ggtgatgcgc
     1141 gtcagggtat agtcgatgaa ttgcgcgctc ttct
//
LOCUS       DASTJJ010000661         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_949051, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000661 DASTJJ010000000
VERSION     DASTJJ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949051"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..978
                     /gene="hemB"
                     /locus_tag="VFR02_08110"
     CDS             7..978
                     /gene="hemB"
                     /locus_tag="VFR02_08110"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET9870443.1"
                     /translation="MAFPIQRLRRLRRTPGLRLLTQETRLAPSDFILPLFVAEGLSKP
                     EPIPSMPGVDRHSLDSLVREASRAHVLGVPAVLLFGVPKKKDVKASEAYRPEGIVQKA
                     VRRLKREIPSLVVITDVCACEYTSHGHCGLLQGKEVANDPTLGLLAKIALSHARAGAD
                     LVAPSDMMDGRVAAIRAGLDAHGFTQTPILSYAAKFASSFYGPFRDAAGSAPAFGDRR
                     SYQMNGANAREALREIAADVEEGADMIMVKPALTALDVIARAREKFQLPLWAYHVSGE
                     YAMVKAAAQKGWLDETGAMVESLTAIKRAGADRILTYWAKEAAKFFR"
     gene            1092..>1822
                     /locus_tag="VFR02_08115"
     CDS             1092..>1822
                     /locus_tag="VFR02_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9870444.1"
                     /translation="MKPRKYSLRNSEKLFAEAQKHLVGGVNSPVRAFRGVGGTPIFFA
                     RGKGPYLIDVDGNRYLDLVGSWGPLILGHLDLEVANRVRRTLRKGFTFGAPTGLETDL
                     AKEVKKAFPSIEKVRFVNSGTEATFSALRLARAATGRKRILKFDGCYHGHGDSFLVAA
                     GSGPATLGLPDSPGVPAESAALTLSVPYNDLGAVEKAFAQFSDIAAVFVEPVAGNMGV
                     VPPEPGFLAGLRELTRKHGALLVFD"
BASE COUNT          306 a          592 c          598 g          326 t
ORIGIN      
        1 gtgaccatgg cattccccat tcaacgctta cgccgcctgc gccgtacccc cggcttgagg
       61 ctcttgaccc aagaaacccg cctggccccc tccgatttca tcctgcccct ctttgtggcc
      121 gagggtcttt ccaagcccga acccatccct tccatgcccg gtgtggaccg ccacagcctg
      181 gattcgctcg tgcgggaggc ttcccgggcc catgtgttgg gggtgccggc tgttttgctc
      241 ttcggggtgc ctaaaaagaa ggacgtgaag gcctcggaag cctaccggcc cgagggcatc
      301 gtgcagaagg ccgtgaggcg cctgaagcgg gagatcccct ccttggtggt catcaccgac
      361 gtttgcgcct gcgaatacac ctcccacggt cattgtgggc tgctgcaggg caaggaagtg
      421 gccaatgacc ccacgctggg cctgctggcc aagatcgccc tgtcccacgc ccgggcgggg
      481 gccgatctgg tggccccttc ggacatgatg gacggccggg tggcggccat ccgcgccggg
      541 ctggacgccc atgggttcac ccaaacgccg atcctcagtt acgccgctaa gtttgcttcc
      601 tctttttatg ggcctttccg ggacgcggcc gggtccgcgc ccgccttcgg cgaccgccgt
      661 tcctaccaaa tgaacggcgc caacgcgcgg gaagccctgc gggagatcgc cgccgatgtg
      721 gaggaggggg cggatatgat catggtcaag cccgccttga cggccctgga tgtgatcgcc
      781 cgggcccggg aaaagttcca actgcccctt tgggcctatc acgtatcggg ggagtatgcc
      841 atggtgaaag ccgcggccca gaagggctgg ctggacgaga cgggcgccat ggtggaatcc
      901 ctcacggcca tcaagcgcgc cggcgccgac cgcatcctca cctattgggc caaggaagcg
      961 gccaagttct tcagataaag gccaaagcgg gtccgcggct tttcttcgac gccgttcttg
     1021 gtgcgtccgg ggaggggagc ggttaaaatc accgggccgc gtcatcacat cccattcttc
     1081 aaagagaagg catgaaaccc cgcaaatact ccctgcgcaa ttccgaaaaa ctcttcgccg
     1141 aggcccaaaa gcacctggtg ggcggggtca acagccctgt gcgggccttt cgaggcgtgg
     1201 gcgggacgcc catcttcttc gcccggggca aggggcccta cctgatcgac gtggacggga
     1261 accggtacct ggacctggtg ggttcctggg gcccgctgat cctgggccac ctggacctgg
     1321 aggtggccaa ccgggtgcgc cggaccctgc gcaagggttt cactttcggg gcgcccaccg
     1381 gactggaaac ggacctggcg aaggaagtga agaaagcctt tccttccatt gaaaaggttc
     1441 ggttcgtcaa ctcagggacg gaagccacct tcagcgccct gcggctggcc cgggcggcca
     1501 cgggcaggaa gcgcatcctg aagttcgacg gctgctacca cgggcacggc gacagtttcc
     1561 tggtggcggc gggcagcggg ccggccacgc tgggattgcc cgacagcccg ggggtgccgg
     1621 ccgaatccgc cgccctgacc ttgagcgtgc cctataacga cctgggggcc gtcgagaaag
     1681 cgtttgccca attttccgac atcgcggcgg tcttcgtgga gccggtggct ggtaatatgg
     1741 gggtggtccc ccccgagccc ggcttcttgg ccggcctgcg ggaactgacc cgaaagcacg
     1801 gcgccctttt ggtgttcgac ga
//
LOCUS       DASTJJ010000662         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_580049, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000662 DASTJJ010000000
VERSION     DASTJJ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580049"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..2021)
                     /locus_tag="VFR02_08120"
     CDS             complement(153..2021)
                     /locus_tag="VFR02_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HET9870445.1"
                     /translation="MPTLEAAPAEWTLGSFLKRYPWPEDWLKKGKPMHFFWKFDLPVP
                     VEKLWPYLADLSTFNRRVGLGAMKFTEKNGRLQGFAVNVGTRMVWEEVPWEWEYGKEF
                     SHARIYSEGLPYYMRARYLFEEPQPGKTRLIVYLSWIPRGLKARLLINIGLKQIKKGY
                     VKALKDIVRAIQGGQAVPAPAPPVKLTPAAQARLQAIGRALVEKGVAPALAKKLVQFV
                     ETTPDEDIYRIRIKPLARDWHFSQKEVLTAFLVGTREGLFKLTWDVICPHCRGVRNEV
                     TTLGELPKRGTCEACQVDFDATAFNTLEVTFHAHPSLRAVEKRVYCAAEPGTKPHIKL
                     QKTLKPQDRLKLGTLLGSGRYRLRIIGRELYNLLDVEDQVEAREIAWDDGLSEQNLKS
                     AYFPTLALENRSAQPTTFVLEESQVDQDTLRPVDLFNFQNFRDLFSQEALASELKLEI
                     GVQTILFTDLVGSTQFYEMEGDTVAFTEVKEHFNKAYEVVLKHDGAIVKTIGDAVMAA
                     FSHQLDAMKAALELQRWFNGKNPATRLRLRVTLHTGSCLAVNLNSAIDYFGNTVNLAA
                     KIQAVAGAGEIAFTERVLEDQALRAHLDSQKIPLEKVDFEMKWSKKTIPVYRMRVE"
     gene            complement(2110..>2284)
                     /locus_tag="VFR02_08125"
     CDS             complement(2110..>2284)
                     /locus_tag="VFR02_08125"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9870446.1"
                     /translation="ETMAFVNAVAYIAHREDHHPDLEVGYKTCRVRYTTHAIGGLSDN
                     DLICAAKVEELAR"
BASE COUNT          402 a          704 c          723 g          455 t
ORIGIN      
        1 tctcaccacc gaccaaccgg ccgccttgaa tacgatgctg gaccagaacg aacaggccat
       61 ccagggcgct tccggcgccc tctgaccgtg cggtaaaaca aaaaaggcgc ccgaggggcg
      121 ccttttttgt tcgacctgcc ggaccttctg gcctactcga ccctcatccg ataaaccggg
      181 atggtcttct tggaccattt catctcgaaa tccaccttct ccaacgggat cttctgggaa
      241 tccaagtggg cccttaaggc ctggtcttcc agcactctct ccgtaaaggc gatttccccc
      301 gcgccggcca ccgcctggat cttggccgcc aggttcaccg tgttgccgaa ataatcgatg
      361 gcgctgttca agttcaccgc caggcaggaa cccgtgtgca gcgtgacgcg caacctcagg
      421 cgcgtggccg gattcttgcc gttgaaccag cgctgcagct ccagcgcggc cttcatggcg
      481 tccaactggt gcgagaaggc ggccatgacc gcgtcgccga tggtcttcac gatggccccg
      541 tcgtgcttga ggaccacctc ataggccttg ttgaaatgct ccttcacctc ggtgaaggcc
      601 acggtgtcgc cttccatctc gtagaactgg gtggacccca ccaggtcggt gaaaaggatg
      661 gtctgcacgc cgatctccag cttcaattcg gaggccaggg cctcctgcga gaagaggtcg
      721 cggaagttct ggaaattgaa caggtccacg ggacgcaagg tgtcctggtc cacctggctt
      781 tcttccagca cgaaggtggt gggctgggcg gagcggttct ccagggccag cgtggggaaa
      841 taggcgctct tgaggttctg ctcgctcaag ccgtcgtccc aggcgatttc ccgggcctcc
      901 acctggtcct ccacgtccag caggttgtag agttcccggc cgatgatccg caggcggtag
      961 cggcccgacc ccagcaaggt gcccagcttg agccggtcct ggggcttcag ggtcttctgc
     1021 agtttgatgt ggggcttggt gcccggctcg gcggcgcaat agacccgctt ttccacggcc
     1081 cgcagggaag gatgcgcgtg gaaggtgacc tcgagcgtgt tgaaggcggt ggcgtcgaag
     1141 tcgacctggc aggcctcgca ggtgccgcgt ttgggcagct cgcccagggt ggtgacctcg
     1201 ttgcgcacgc cccggcagtg ggggcagatg acgtcccagg tcagtttgaa gagcccttcc
     1261 cgggtgccca ccaggaaggc cgtgagcact tctttctgcg aaaaatgcca gtcccgggcc
     1321 aggggcttga tgcggatgcg gtagatgtcc tcgtcgggtg tggtttccac gaattggacc
     1381 agtttcttgg ccagggcggg cgccacgccc ttttccacca gcgcccggcc gatggcctgg
     1441 agccgggcct gggccgcggg cgtcagcttg acggggggcg cgggggccgg cacggcctgc
     1501 ccgccctgga tggcccggac gatgtccttc agcgccttga cgtagccctt cttgatctgc
     1561 ttcaagccga tgttgatgag gagcctggcc ttcaggcccc gggggatcca ggaaagataa
     1621 acgatcagcc gggtcttgcc gggctggggt tcctcaaaaa ggtagcgggc ccgcatgtaa
     1681 taaggaagcc cctcgctata gatccgggca tggctgaatt ccttcccgta ttcccattcc
     1741 cagggcacct cttcccagac catgcgggtg cccacgttga ccgcgaagcc ctgcaggcgc
     1801 ccgttcttct cggtgaactt catggcgccc aatcccaccc ggcggttgaa ggtcgagagg
     1861 tcggccaggt agggccaaag cttttccacc ggcacgggca ggtcgaattt ccagaaaaag
     1921 tgcatgggct tgcccttttt caaccagtcc tccggccaag ggtaccgctt taaaaaggat
     1981 cccagggtcc attcggcggg cgcggcttcc agggtcggca tggatactct ccaaacaagg
     2041 gatggggaag acatcaagga tataagcttt aaaagacggc ggggttacag gatcccggaa
     2101 taatcggacc tatcgggcca gttcttccac cttggccgcg cagatcaggt cgttgtccga
     2161 caggcccccg atggcgtggg tcgtgtagcg cacccggcag gtcttgtagc ccacttccag
     2221 gtcggggtgg tggtcctccc gatgggcgat ataggccacg gcgttcacga aggccatggt
     2281 ttcg
//
LOCUS       DASTJJ010000663         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1315105, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000663 DASTJJ010000000
VERSION     DASTJJ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315105"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFR02_08130"
     CDS             complement(<1..510)
                     /locus_tag="VFR02_08130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870447.1"
                     /translation="MIPTYVTREETDELERLGASKRVLELGAQFGYSTIHLARSAAEV
                     WSVDWHFGDDQAGEMDTLSAWAGHTANLRKAGRVVGIIGRFEMVLPLLQPASFDLIFH
                     DGYHGRAEVMRDARLALPLLSWRGIFCAHDWGSFSGVQGLATVMGSPDYVMGSLAVWL
                     NPGGRWGPGE"
     gene            898..1140
                     /locus_tag="VFR02_08135"
     CDS             898..1140
                     /locus_tag="VFR02_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870448.1"
                     /translation="MLNLDDAAVDWFMGARALGITWANALEDRRLADERAQGGPVTFL
                     EALASDLIESCDLAGITYYHEGAFYFTPNDPWGLGL"
     gene            1137..1325
                     /locus_tag="VFR02_08140"
     CDS             1137..1325
                     /locus_tag="VFR02_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870449.1"
                     /translation="MTSRRRPAYIEERITYHLARQRARREHPQPCTCHYCIDSATREA
                     MIRRNIERGQATYEEIPF"
BASE COUNT          280 a          578 c          509 g          287 t
ORIGIN      
        1 ttctccgggg ccccagcggc cgcctgggtt gagccagacc gccaacgagc ccatcacgta
       61 atcgggcgag cccatcaccg tagccaggcc ttggaccccg gagaaactgc cccagtcgtg
      121 ggcgcagaag atgccccgcc aactgagcag agggagggcc aggcgagcgt cccgcatcac
      181 ctccgcccgg ccgtggtagc cgtcgtgaaa gatgagatcg aagctggccg gctggagtag
      241 gggcagcacc atctcaaagc ggccgatgat gcccaccacg cgccccgcct tgcgcaggtt
      301 cgcggtatga cccgcccagg cgctcagggt gtccatctcg cccgcctgat catccccgaa
      361 gtgccagtcc acgctccaca cctcggccgc cgagcgggcc aggtggatgg tcgaatagcc
      421 gaactgagcc cccagctcca gaacccgctt gctggctccc agccgttcca actcgtcggt
      481 ttcctcgcgg gtcacgtagg tggggatcat ggctcggggt agaggttgac gaccacacgg
      541 cctccaggag ccccccaaac ccggtagtag ggaactgggc cgttggggtc gatcccggcc
      601 ctctccaggg ctacagagtc gatgtatatg tcaataccct tgcagcgggg tcggcggacg
      661 ccgcgtatgc gctgggctcg gtctcgaagt ctcatcagca ctacctccgc cccggagcat
      721 acaccctccg gccatggtat ggtgcggaca acgggccgcc cctggcttcg acgcggccag
      781 cctgggcact acctccaaag cagtaccccc gggcggcccg cctctctgca actcccctag
      841 acaaaaccct tgacgagccc gggccttgga gtatgattcg cggtggaggt agtgcatatg
      901 ctgaatcttg acgacgccgc cgttgattgg tttatggggg cccgtgccct cgggattacc
      961 tgggctaacg ccctggagga tcgccgcctg gccgacgaac gggcccaggg cgggccggtg
     1021 actttcctgg aagccctggc ctccgacctg atcgaatctt gcgacctggc agggatcacc
     1081 tactatcacg agggcgcgtt ctacttcact cccaacgacc cctgggggct cggtctgtga
     1141 cctctcgccg ccgccccgcc tacatcgagg agcgcatcac ctatcacctg gcgcggcagc
     1201 gggcccgccg cgaacacccc cagccctgta cctgtcatta ctgcatcgac tcggctaccc
     1261 gcgaggccat gatccgccgc aacatcgagc gcggccaggc gacctatgag gagatcccgt
     1321 tttgagaact gcacgggctc tggcggccca tgagactgag cgatttcttt ggcaggggcc
     1381 ggaggccaag gtcgatgcgg ctattcgccg tttctcgccg cttcccgcgg ggtgctggtt
     1441 caggtcttac gttcatcccg agcggctagg ctgggtccag ctttaccggg tgcgaccgtg
     1501 atccgctttc cgcgcaagga cgccgacata aacgccctga ctcaagagct ggtcaatcat
     1561 cccgcccagg tcatggtcgc ccgactctgc gcccaggacc tcggattacc tgacggcgac
     1621 cctttccgta agctccgggt ctggctggcc cggg
//
LOCUS       DASTJJ010000664         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1192564, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000664 DASTJJ010000000
VERSION     DASTJJ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192564"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /gene="hprK"
                     /locus_tag="VFR02_08145"
     CDS             <1..758
                     /gene="hprK"
                     /locus_tag="VFR02_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET9870450.1"
                     /translation="QRRAILHKIFSYAMPCVIVTHKMKPPADMVAEAKRQKIPLFTTP
                     TPTTKFVSEVSVFLEEEFAPETTIHGTLLDIYGVGVLILGNSGIGKSECALELVERGH
                     RLVADDLVYIKRLANRILMGSRSEIIKHHMEIRGLGIINVESLFGVGAIRDKKRIELV
                     VRLEDWDNKKEYDRLGLNPAKHKIFDVEIPMFLIPVRPGRNLAVIIEVASMNHRLMKL
                     GYFTAQEFADRVSRMMQDENIRRAMPSRRLGKP"
     gene            758..>1628
                     /locus_tag="VFR02_08150"
     CDS             758..>1628
                     /locus_tag="VFR02_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET9870451.1"
                     /translation="MPRLRWLYPGMRLKRWFLLMAFSVLGLAMGFSGLLGRRLHGVHY
                     NPPLIMEFADNLKHLKFADFLLILLCLSLGVYAVRRALFAVLTVLLPDREKEFMNIAY
                     RQARLKRGPRIVCVGGGTGLPNVLYGLKQFTSNLTAVVTVADDGGSSGRLRKDFGTPP
                     PGDIRNCLVALADQQTLLNDLFQYRFKGHGDLKGHSFGNLFITVMGEITGDFGKAVEE
                     SSKVLATRGTVMPVTFDSMTLQARFKNGRVVTGESAIPEVKSPINEVMLKEKRIRPNK
                     EVLKAILEADAIVL"
BASE COUNT          291 a          562 c          503 g          272 t
ORIGIN      
        1 cccagcgccg ggccatcctc cacaagatct tctcctacgc catgccctgc gtgatcgtga
       61 cccacaagat gaagccgccc gccgacatgg tggccgaggc caagcgccag aagatccccc
      121 tcttcaccac cccgaccccc accaccaagt tcgtgtccga ggtgtcggtg ttcctggagg
      181 aggagttcgc ccccgagacc accatccacg ggacgctcct ggacatctac ggcgtgggcg
      241 tgctcatctt gggcaactcg ggcatcggca agagcgagtg cgcgctggaa ctggtggaac
      301 gcggccaccg gctggtggcc gacgacctgg tctacatcaa gcgcctggcc aaccgcatcc
      361 tgatgggctc gcggtccgag atcatcaagc accacatgga gatccgcggc ctgggcatca
      421 tcaacgtgga aagcctcttc ggcgtgggcg ccatccgcga caagaagcgc atcgagctgg
      481 tggtgcgcct ggaggattgg gacaacaaga aggaatacga ccgcctgggg ctcaacccgg
      541 ccaagcacaa gatcttcgac gtggagatcc ccatgttcct catcccggtg cggcccggcc
      601 gcaacctggc cgtcatcatc gaggtggcct ccatgaacca ccggttgatg aagctgggct
      661 atttcacggc ccaggaattc gccgaccggg tctcccgcat gatgcaggac gagaacatcc
      721 gccgggccat gccttcccgc cggctcggga agccctgatg ccgcgactgc gctggcttta
      781 cccgggcatg cgcctgaagc ggtggttcct gctcatggcc ttttcggtgc tgggcctggc
      841 catgggcttt tccggcctgc tgggccgccg cctgcacggg gtgcattaca acccgcccct
      901 catcatggag ttcgccgaca acctcaagca cctcaagttc gccgacttcc tgctcatcct
      961 cttgtgcctc agcctgggcg tctacgccgt gcgccgggcc cttttcgccg tgctgacggt
     1021 cctcctgccc gaccgggaaa aggagttcat gaacatcgcc taccggcagg cgcggctgaa
     1081 gcgcgggccc cgcatcgtgt gcgtgggggg cggcacgggc ctgcccaacg tgctctacgg
     1141 cctcaagcag ttcaccagca acctgaccgc cgtggtgacg gtggccgacg acggcggcag
     1201 ctcggggcgc ctgcgcaagg atttcggcac cccgccgccc ggcgacatcc gcaactgcct
     1261 ggtggccctg gccgaccagc agacgctcct caacgacctg ttccaatacc gcttcaaggg
     1321 ccacggcgac ctgaaggggc acagtttcgg caacctgttc atcacggtca tgggcgagat
     1381 cacgggcgat ttcggcaagg ccgtggagga gtccagcaag gtgctggcca cccggggcac
     1441 ggtcatgccc gtcacctttg attcgatgac gctccaggcc cgcttcaaga acggccgggt
     1501 ggtgaccggg gagtccgcca tccccgaggt caagtcgccc atcaacgagg tcatgctcaa
     1561 ggaaaagcgc atccgcccca acaaggaggt gctgaaggcc atcctggagg ccgacgccat
     1621 cgtgctgg
//
LOCUS       DASTJJ010000665         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1192647, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000665 DASTJJ010000000
VERSION     DASTJJ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192647"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFR02_08155"
     CDS             complement(<1..545)
                     /locus_tag="VFR02_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9870452.1"
                     /translation="MRHRSFLIFFAVFLLVLFGLHYYLWARLVRDTGLRMPWRALATA
                     AIAGFGLLIPASFILMRAAPRTPAPLFWAVYLWMGLAFFLVVLLAVGDLARLPLWLSD
                     LARRAPEDPGRRQFLGRLVAGTALLGAGGLSMAGLFGAAAGAIRVKRVRVGLSKLSPD
                     LEGFRIVQVTDLHVGPTIGLS"
     gene            complement(612..800)
                     /locus_tag="VFR02_08160"
     CDS             complement(612..800)
                     /locus_tag="VFR02_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870453.1"
                     /translation="MPWRCPACRNRILRSARRCPVCGCSFSRFDPTGPFLWLFFLAAL
                     VFTGFILFHLSPETVAKL"
     gene            complement(897..>1561)
                     /locus_tag="VFR02_08165"
     CDS             complement(897..>1561)
                     /locus_tag="VFR02_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870454.1"
                     /translation="LNFVPFPAVGEVGEKDILKYSWMLSESHRVLMYDPAKRRLIDEL
                     YTCSPEKDGAIMQERTNKRLGKPRKSVSLHEIVSKEMALKEEPDKLFKAVEAASRKLR
                     LAPDENYCVSAVFFDIPDRKLEEDSLEVMLTQALNKDDQKPVIESACRYDPQGYTYLH
                     QSPKHFAGLHSYPGTLKSAHLSLIGSGNAVVSVLEDVEDVVYKRKKSEPTISIKIVAE
                     GF"
BASE COUNT          299 a          461 c          563 g          238 t
ORIGIN      
        1 taagagaggc cgatggtggg gcccacgtgc aggtcggtga cctggacgat gcggaacccc
       61 tccaggtcgg gggacaactt gctcaacccc acgcgcaccc gcttcacccg gatggcgccc
      121 gcggcggccc cgaaaagacc ggccatcgac aggcccccgg cgcccaagag ggccgtgccc
      181 gccaccaagc ggcccaggaa ctgccggcgg cccgggtctt ccggcgcgcg gcgcgccagg
      241 tccgaaagcc aaaggggcag gcgcgccagg tcgccgacgg ccaagagcac caccaggaaa
      301 aaagccaggc ccatccagag atagacggcc cagaagagcg gcgcgggcgt ccggggggcg
      361 gcgcgcatca ggataaaact ggcggggatg agcagcccga atccggcgat ggccgccgtg
      421 gccagggcac gccaaggcat cctcaggccc gtatcgcgca ccagcctcgc ccaaaggtaa
      481 tagtgcaggc cgaagaggac gagcaggaag accgcgaaga agatgaggaa agaacggtgg
      541 cgcatgggac ctcgggtgtg gaagatgcgg cccgaaaagg ttaacggaag ggggcttgga
      601 ttctcaaggg gtcaaagctt ggccacggtc tccggcgaca ggtggaaaag gatgaagccc
      661 gtgaaaacca gcgcggccag gaaaaagagc cataagaagg gccccgtggg gtcgaagcgc
      721 gagaaggaac aaccgcacac cgggcaacgg cgggcggatc tcaggatgcg gttgcggcat
      781 gcggggcagc gccagggcat gggggcctcg ggttcgggga tggggcccgt tgggacccgg
      841 attttggacg cttcaggggg ggctggggtt acgaaaaagg cggggccggc tgggacctag
      901 aagccttcgg cgacgatctt gatgctgatg gtgggctcgg acttttttcg cttgtagacc
      961 acgtcctcca cgtcctccag caccgacacc acggcgttgc cgctgccgat gaggctcagg
     1021 tgggcggact tgagggtgcc gggatagctg tgcagcccgg cgaagtgctt gggcgactgg
     1081 tgcaggtagg tatagccttg cgggtcgtag cggcaggccg actcgatgac cggcttctgg
     1141 tcgtccttgt tgagggcctg ggtgagcatg acctccaggc tgtcctcttc cagcttgcgg
     1201 tccgggatgt cgaagaagac cgccgacacg cagtagttct cgtcgggggc caggcgcagc
     1261 ttgcgggaag ccgcctccac cgccttgaag agcttgtccg gttcctcttt gagggccatt
     1321 tccttgctga cgatctcgtg caggctcacg ctcttgcggg gcttgcccag gcgcttgttg
     1381 gtcctttcct gcatgatggc cccatccttc tccgggctgc aggtgtagag ctcgtcgatc
     1441 aggcggcgct tggcggggtc gtacatgagc acccggtggg actcggacag catccaggaa
     1501 tacttgagga tgtccttctc gcccacttcc cccacggccg ggaagggcac gaaattgagg
     1561 a
//
LOCUS       DASTJJ010000666         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_337193, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000666 DASTJJ010000000
VERSION     DASTJJ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337193"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFR02_08170"
     CDS             <1..476
                     /locus_tag="VFR02_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9870455.1"
                     /translation="TYQATVSLNTAVTGATGGSGVPIVVVGAPVSGGTITVNQPTDTP
                     TPTQTATVTPTPTITNTPIPFVFTVSQNVLTDATSPVSIHASYPQSGKYEMKVYNSVG
                     EFIKDLDPGTNPSGQDHWFTWDGTNYLGQKCASGIYVIYYLQPLGVKEAKILLLR"
     gene            559..>1331
                     /locus_tag="VFR02_08175"
     CDS             559..>1331
                     /locus_tag="VFR02_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9870456.1"
                     /translation="MEKRVLGQGLEVPALGLGCMGMTFAYHPADAAESLRVLDRSLEL
                     GMNFWDTAQSYGPFTNEELLGRALKGRRDKVVLATKFGWKGGRAVPEALDGSPENTRD
                     SVEGCLRRLGTDHLDLLYLHRADPQRPIEETWGAMAALVREGKVKYLGLSEVGPKTLR
                     RAHTLHPVTALQSEYSLWETAIEAAILPTLRELGVGLVPFSPMGRGFLTGKIRSPEDL
                     GEGDMRRNLPRFQGKNFQANLRLVEAVGALAAARQATPG"
BASE COUNT          235 a          465 c          419 g          212 t
ORIGIN      
        1 gcacctacca ggccaccgtc tccttgaaca ccgcggtcac gggcgccacg ggaggttcgg
       61 gcgtgcccat cgtggtcgtc ggggccccgg tgtcgggcgg gaccatcacc gtcaaccagc
      121 ccacggacac cccgaccccc acccagacgg ccaccgtaac gcccacgccc accatcacca
      181 acacgcccat ccccttcgtc ttcaccgtgt cccagaacgt cctgaccgac gccaccagcc
      241 cggtgtccat ccacgccagc tatccccagt ccggcaaata cgaaatgaag gtctataatt
      301 cggtcggcga gttcatcaag gacctggacc cgggcaccaa cccctccggg caggaccact
      361 ggttcacctg ggacggcacc aactacctgg gccaaaagtg cgccagcggc atctacgtca
      421 tctactacct ccagcccctg ggcgtaaagg aagccaagat cctgctgctg cgctgacgcc
      481 gcccccgcct tgcaataaga ccggacccct gccatcctgg gtccggtctt tttttattcc
      541 cgcctccgga ggccgttcat ggaaaagcgc gtgttggggc aaggcctgga ggtccccgcc
      601 ttggggctgg gctgcatggg catgaccttc gcctaccatc cggccgacgc ggccgagtcc
      661 ctccgggtgt tggaccgctc gctggaactg gggatgaact tctgggatac ggcccaaagc
      721 tacggcccct tcaccaatga ggaactgctg ggccgggccc tgaaggggcg ccgggacaag
      781 gtggtcttgg ccaccaaatt cggctggaag gggggccggg ccgtcccgga ggccctggat
      841 gggtcccccg agaacacccg ggattcggtc gaagggtgcc tgcggcgcct ggggaccgac
      901 cacttggacc ttttatacct gcaccgggcg gacccccagc ggcccatcga ggagacctgg
      961 ggtgccatgg cggcgctggt gcgcgagggc aaggtgaaat acctgggttt gagcgaggtc
     1021 ggccccaaga ccctgcgccg ggcccacacc ctgcatcccg tcacggcgtt gcagagcgaa
     1081 tattcgctct gggaaacggc catcgaggcc gccattctgc ccaccttgcg ggaattgggc
     1141 gtcgggctgg tgcccttcag ccccatgggc cggggattcc tgaccggcaa gatccgttcc
     1201 cccgaggacc tgggggaagg cgacatgcgg cgcaacctgc cccgcttcca agggaagaat
     1261 ttccaggcca atttgaggtt ggtggaggcg gtcggcgcct tggcggcggc acggcaggcc
     1321 actccgggac a
//
LOCUS       DASTJJ010000667         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_337363, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000667 DASTJJ010000000
VERSION     DASTJJ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337363"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFR02_08180"
     CDS             complement(<1..273)
                     /locus_tag="VFR02_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET9870457.1"
                     /translation="MKGRITIKRAYEPASKLDGRRFLVDRLWPRGIKKEKLKMTAWCK
                     DVAPSSGLRTWFGHDPKKWDLFRERYIKELRANRQALQPLLAAAPKG"
     gene            complement(270..983)
                     /locus_tag="VFR02_08185"
     CDS             complement(270..983)
                     /locus_tag="VFR02_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9870458.1"
                     /translation="MRLVGAAPMFPMQDLTAPSGKALALYALIGCLAGLFAIGITKAV
                     YAVEDGFEHLPLHWKWWPALGGVAVGTIGLFSPKTMGVGYDNIEHLLKGDFTFSLIIG
                     LTFFKFLSWCFSLGSGTSGGTLAPLFTIGGGLGFLMAGGLAWVFPLAGLDPKVGALVG
                     MAALFTGASRAFLTSVIFAFELTHQPLGLLPLFLGCSGAYLVSCLFMRNTIMTEKIAR
                     RGVQVPHGYMGHHHGGNGT"
BASE COUNT          236 a          298 c          311 g          180 t
ORIGIN      
        1 gccttttgga gcggccgcca acaagggttg aagggcttgc cggttggccc ggagctcttt
       61 tatgtaacgc tcccggaaca ggtcccactt cttggggtca tggccgaacc aggtccttaa
      121 cccgctggaa ggggccacgt ccttgcacca agcggtcatc ttcagttttt cttttttgat
      181 gcccctgggc caaagccggt cgaccaggaa ccttcggcca tccagtttgg aggccggttc
      241 gtaagctcgt ttgatcgtga tccgtccctt caagttccgt tacccccatg gtgatggccc
      301 atatatccat gcggtacctg gacaccgcgc cgggcgatct tttcggtcat gattgtgttg
      361 cgcatgaaaa ggcaggagac caggtaagcc ccggagcaac cgaggaagag gggaaggagg
      421 cccaggggtt ggtgggtcaa ctcgaaagcg aagatcaccg aggtcaggaa tgcccgggag
      481 gccccggtaa aaagggcggc catgcccacc agggccccga ctttggggtc caagcccgca
      541 agggggaaga cccaggccaa tccacccgcc atcaagaatc ccaaacctcc gccgatggtg
      601 aaaagcgggg ctagggtgcc ccctgaggtg ccgctcccaa gggaaaagca ccaagaaaga
      661 aacttgaaaa aagtgaggcc tatgatgagc gaaaaggtga aatccccttt caacagatgc
      721 tcgatgttgt cataacctac ccccatggtc ttgggcgaaa agagtccgat ggtccctacg
      781 gctacccctc ccaaggcggg ccaccatttc caatgcaacg gaaggtgctc gaagccgtcc
      841 tctacggcgt aaacggcttt cgtgatccca atggcgaaga ggcccgccaa gcacccgatc
      901 aaggcataga gagccaaggc tttgccggag ggagcggtca ggtcctgcat gggaaacatg
      961 ggagccgcgc ccaccagccg cattcgcaga gccaacgcgg tggcgctggc gaaggaaacg
     1021 ggcag
//
LOCUS       DASTJJ010000668         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1437678, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000668 DASTJJ010000000
VERSION     DASTJJ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437678"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VFR02_08190"
     CDS             <1..1450
                     /locus_tag="VFR02_08190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9870459.1"
                     /translation="SRQLDDMHKGYGSDAEFNQALAEQGITLDDLKQDLKTQLERRLK
                     AQHALRQKQQDLPQSLVVTDSQARQYYDQHQEDFNQAHFAIILFKMNPGANPVYVKEV
                     RAQAQHVLDDLKAGGDFAAAAKKYSEDPGSADRGGDVGMVARSALEPELAKGVFSTPV
                     GGMSLVRGADGFYIVKVLSRQQADFDSVASQIKDQLRASNQDQALRDWVESLKKKVYV
                     QYSKSLAAEAAADLPYNPLNATPSAPLTVAPAGAAAPAAAAGGAVATSSPVPTSTVVP
                     VYSSLPDEGNTALDLTISPWGYGTADLAQNYGAGTDVSQGFPFGLEAQLGLETTLDPQ
                     LQLGAFLQVMDKITPTVTAGGTTAHWEEAAVGLLAGPKVLLPLSPGLNLDLYAQGGYF
                     FLFGTAAKFDGAQTGSVYLDGSNFGGQVGADLEFFLDYEKTFALDLGCAYRRLLVNPT
                     VHGAVAGYPLPGYQVDFSGFKSVLGFRFYLDK"
     gene            1705..2007
                     /locus_tag="VFR02_08195"
     CDS             1705..2007
                     /locus_tag="VFR02_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HET9870460.1"
                     /translation="MRLDLYLKLMGFAKTRMAAKRLCDARRVLVADRSVKPSLELAGG
                     EHLTLFFPQKEVRAQVLALPPGKSVAKKDRPLYVLAREEGWRPQEPGGGGPGASLE"
     gene            2032..2856
                     /locus_tag="VFR02_08200"
     CDS             2032..2856
                     /locus_tag="VFR02_08200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9870461.1"
                     /translation="MRAADYALIERSIRTLADHYAENPPLARLAKDAGLSPFHFQRLF
                     KRWAGVSPKRFAQYLSAENAKRRLRSAKSLLDATFESGLSSPGRLHDLLVSVEAMTPG
                     DYRRRGRDLVIEYGTFPSPFGDCLAALTPRGLCGLAFLDARGPGHALRDFKRRWPLST
                     FRPNPRRAARVGKQVFARKGKARPRLVLRGTPFQLKVWEALLRVPEKALVTYGELARR
                     VGHPKASRAVGTAVGRNPVAYLIPCHRVIRETGVLGDYHWGAARKKALVGWEGARK"
BASE COUNT          568 a         1094 c         1001 g          570 t
ORIGIN      
        1 cagccgccaa ttggacgaca tgcacaaggg ctacgggtcc gacgccgaat tcaaccaggc
       61 cctggccgaa cagggcatca ccctggacga cctcaagcag gacctcaaga cccaattgga
      121 gcgccgcttg aaggcccaac acgccctacg ccagaagcag caagacctgc cccagagcct
      181 ggtggtgacc gattcccagg cccgccagta ttacgaccag catcaggaag atttcaacca
      241 ggcccacttc gccatcatcc tcttcaagat gaacccgggg gccaacccgg tctacgtgaa
      301 ggaagtgcgg gcccaggccc agcatgtgct ggacgacctg aaggcagggg gcgacttcgc
      361 cgccgccgcc aagaaatatt ccgaggaccc gggttcggcc gaccgcggcg gggacgtggg
      421 catggtggcc cggtccgccc tggaacccga gctggccaag ggcgtctttt ccaccccggt
      481 gggggggatg tcgctggtgc ggggcgcgga cgggttctat atcgtgaagg tcctttcccg
      541 ccaacaggcc gacttcgaca gcgtggcctc ccagatcaag gaccagctgc gggcttccaa
      601 ccaggaccag gccctgcggg actgggtgga gtcgctcaag aagaaggtct atgtgcagta
      661 cagcaagtcc ttggccgccg aggcggccgc cgaccttcct tataatccct tgaacgccac
      721 cccctcggcg cccctgaccg tggcccccgc gggcgcggcc gcccccgccg ccgcggccgg
      781 cggcgccgtc gccaccagct ccccggtccc gacgtccacc gtggtgccgg tctattccag
      841 cctgcccgac gaaggcaaca ccgcgttgga cctcacgatc tccccctggg gttacggcac
      901 ggcggacctg gcgcagaact acggcgcggg cacggacgtt tcacaaggct tccccttcgg
      961 cctggaggcc cagttgggct tggagaccac gctggatccc caactgcagt tgggggcctt
     1021 cctgcaggtg atggacaaga tcacgcccac ggtcacggcc gggggcacca cggcccactg
     1081 ggaagaggcc gccgtcggcc tcttggcggg ccccaaggtg ctcttgcccc tgtcgccggg
     1141 cctgaacctg gacctctacg cccagggcgg ttatttcttc cttttcggca ccgccgccaa
     1201 gttcgacggc gcccaaacgg gatcggtgta cctggacggg tccaacttcg gcggccaagt
     1261 gggcgccgac ctggagttct tcctcgatta cgagaagacc ttcgccctcg acctgggatg
     1321 cgcctaccgg cgcctgctgg tgaaccccac ggtccacggc gcggtggcgg gttatcccct
     1381 gccgggctac caggtggact tctcgggctt caagtcggtc ctgggtttcc gcttctacct
     1441 cgacaagtaa aagacgtccc cttaaaaagc cggggcccct tcgggccccg gcttttttta
     1501 tcggaactat ggcttttgca aagcaagcca aagagagctt gaaacaatga taaaaactga
     1561 tgagatctga tttttgaaaa taaagatcgg gctttatcag cttttatcaa tgttccaatt
     1621 gtccttgttt cgcgaaatgc atgattcctt tttttattgg gtcggaggct gaaaagcggt
     1681 tggggggcct gctaaaatcc acctatgcgc ctggacctct acctcaaact catgggattc
     1741 gccaagaccc gcatggccgc caagcgcctg tgcgacgcgc gccgggtgct ggtcgcggac
     1801 cggtccgtca agccgtccct ggaactggcg ggcggcgagc acctgacgct cttcttcccg
     1861 caaaaagagg tgcgggccca ggtgctggcc ctgcccccgg gcaagtcggt ggccaagaag
     1921 gaccggcccc tttacgtgtt ggcgcgggaa gaggggtggc ggccgcaaga accgggtggc
     1981 ggcggcccgg gggcttccct agagtaggcc catccttttt caggaggccc catgcgcgcc
     2041 gccgattacg ccctgatcga acggtcgatc cgaaccctgg cggaccatta cgccgagaac
     2101 ccgcccttgg cccggctggc gaaggacgcg ggcctgagcc ccttccattt ccagcggctc
     2161 ttcaagcgct gggcgggcgt cagccccaag cgcttcgccc aatacctgag cgccgagaac
     2221 gccaagcgcc gcctgcggtc cgccaagagc ctgttggacg cgaccttcga gtccggcctc
     2281 tcctcgccgg gccgcctgca cgaccttttg gtctcggtgg aggccatgac gccgggggat
     2341 taccggcgcc ggggccggga cctggtcatc gagtatggga cctttccctc gcccttcggg
     2401 gactgcctgg cggcgctcac cccccggggc ctctgcggct tggccttcct ggacgcccgg
     2461 gggccgggcc acgcccttcg ggacttcaag cgccgctggc ccctctccac cttccgcccc
     2521 aacccgcgcc gcgccgcccg ggtggggaaa caggtcttcg cccgcaaggg aaaggcccgg
     2581 ccacggctgg tgctgcgcgg cacgcccttc cagttgaagg tctgggaggc gctgctgcgg
     2641 gtgcccgaga aggccctggt cacctacggc gaattggccc ggcgggtggg ccacccgaag
     2701 gcttcccggg cggtggggac ggccgtgggg cgcaaccccg tcgcctacct catcccctgc
     2761 caccgggtca tccgggaaac gggcgtcctg ggggactatc actggggcgc ggcgagaaag
     2821 aaggccctgg tgggatggga aggcgcccgg aagtagggtt tactatataa ttacggaact
     2881 atggcttttg caaagcaagc caaagagaac ttgaaacaat gataaaaact gatgaaatct
     2941 gaattttgaa aataaagatc gggctttatc agcttttatc aatgttccag ttgtccttgc
     3001 tttgcgaagt gcatgattcc gtataattaa ggaccgagcc gccggatcca tcccaaccct
     3061 ttttaggagc tccccatgac cccccggatt ttcgcggccc tcctgcccct tctcgccgcc
     3121 tcctggttgg gcctgccccc ggcggcccgg gccgccacgt ccgccgacta tgacgccgcg
     3181 ggccagcagc tctacaacgc ccgacagtac gcccaggccg ccccctattt ctc
//
LOCUS       DASTJJ010000669         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1682026, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000669 DASTJJ010000000
VERSION     DASTJJ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682026"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1059)
                     /locus_tag="VFR02_08205"
     CDS             complement(73..1059)
                     /locus_tag="VFR02_08205"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9870462.1"
                     /translation="MPKPTKKTKKKAAKGGKSPAQKRKAIVTILALLVAVILCGEAVF
                     FMKGQLNIPRDYPVQLAGSFKGDNQACGPFAPWDIKSGPNWIAMTDQPRERVLIFDLQ
                     GHYLRQIDSKAAGKPDFKEISCLATDGQKTIYVMDTWNALIRYFNVDTGKPMGVLDMT
                     TKGMFGPRGVAWDNGDFIVADTGTHHVDKLSPSGQVLATWDGAGGKTKLLNPIAAAVD
                     SQGRIFVANEHGLKVLSPDGKVLQDEESLPVNDVAVGPGDTVYAALRDSGDVKVFNSQ
                     GKYVGTLSTAGVKDQWIDGVRALTVLPGGDLACVRGGEVDLYHAVPQAAPAH"
     gene            complement(1210..1434)
                     /locus_tag="VFR02_08210"
     CDS             complement(1210..1434)
                     /locus_tag="VFR02_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HET9870463.1"
                     /translation="MKKHYTHGGVTILWQSALCIHCGNCARGLPQVFNPAKRPWIEPA
                     AATGEEIKNQVAQCPSGALSIVETGADGLP"
BASE COUNT          286 a          538 c          550 g          306 t
ORIGIN      
        1 aaaagcatac cgaaaaagcg gcatttgggg gccgccgcgc cgggaaaaac gcgcccgggc
       61 ggcgggaaag gctcagtgcg ccggggccgc ctggggcacc gcatggtata ggtccacttc
      121 cccgccccgc acgcaggcca ggtccccgcc cggcagtacc gtgagggcgc gcacgccgtc
      181 gatccattgg tccttgacgc ccgcggtgga aagggtcccc acatacttgc cttgggaatt
      241 gaagaccttg acgtcccccg aatcccgcag cgccgcgtag acggtgtcgc cggggcccac
      301 cgccacgtcg ttcaccggca gcgattcctc gtcctgcagc accttgccgt cggggctcag
      361 caccttcagg ccgtgctcgt tggccacgaa gatacggccc tgggaatcca cggccgccgc
      421 gatggggttg agcagcttgg tcttgccgcc cgcgccgtcc caagtggcca acacctggcc
      481 ggagggggac agcttgtcga cgtggtgggt gcccgtgtcg gccacgatga agtcgccgtt
      541 atcccaggcc acgccccggg ggccgaacat gcccttggtg gtcatgtcca acacgcccat
      601 cggcttgccg gtgtccacgt tgaaatagcg gataagggcg ttccaggtgt ccatcacgta
      661 gatggtcttt tggccgtcgg tggccaggca ggaaatttcc ttgaagtcgg gcttgcccgc
      721 cgccttgctg tcgatctgcc ggaggtaatg gccctgaagg tcgaagatca gcacgcgttc
      781 ccggggctgg tcggtcatgg cgatccagtt cggaccggac ttgatgtccc aaggcgcgaa
      841 gggcccgcag gcctggttgt cgcccttgaa ggaacccgcc aactgcaccg ggtagtcccg
      901 ggggatgttg agctggccct tcatgaaaaa gaccgcctcg ccgcagagga tgacggccac
      961 cagcagggcc aggatggtga cgatggcctt gcgcttttgg gccggggact tcccgccctt
     1021 ggcggctttt ttcttggttt tcttggtggg cttaggcaag ggcggatcct ccgggatgat
     1081 ataaggaaag cccttgcggg gctttccggg cgttcaagat aacacccgat ccagcggcgg
     1141 acaacgagga atcaagcccc accgaccggt cggggaatga ttcccaggga aaatcgggtg
     1201 aatcgggctt taagggagcc cgtccgctcc cgtttcgacg atggaaaggg cccccgaggg
     1261 gcactgggcc acctggtttt tgatctcctc gccggtggcg gcggccggct cgatccaggg
     1321 ccgcttggcc gggttgaaga cctgcggcaa ccccctcgcg cagttcccgc aatggatgca
     1381 gagcgccgac tgccagagga tggtgacccc gccgtgggtg tagtgttttt tcacatgccg
     1441 acgatccatc cacgcctcct ctgacgccag ataccccgcc ggagcggttt gatcgggcaa
     1501 tgcagggatc gtcatgctga cgccgaccgg gataccggaa gcgtcgatcg gaccttgtcc
     1561 cctcgcaagc gaggcccaaa agatcgaccc gccgatggcc ccatgctcca ggcccggcca
     1621 cgcatcgcat tatagtttgg ggtccttcat ccttttgagg cgcggccagg ccgatccttc
//
LOCUS       DASTJJ010000670         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_337852, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000670 DASTJJ010000000
VERSION     DASTJJ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337852"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFR02_08215"
     CDS             <1..859
                     /locus_tag="VFR02_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870464.1"
                     /translation="AGFRYFVLPQWAADFGASFRSADAKNGALQSVDFHTGLSFFVNS
                     PFKNADKMVWAGGGVTIMQPVTITATDQGAQSLSDIARDEYGDPDLYPLIIDANFADK
                     DKPLILPPGIQLIIPQHPTGEMMAAAHVKAKTPVYKKAADTFRMPELDNLQYRLEPAQ
                     NNQETMLGGQRSELTFRADSLWDIAARPDVYGDPELYPLLVDANFSRLSDPLLLKPGA
                     HLVIPRHPSEREKRMARVKAWTVDYIRWRGVDVTEGQYREWRKERGLPPVDDVTGEVL
                     QPQPADEGN"
BASE COUNT          231 a          371 c          334 g          214 t
ORIGIN      
        1 ggcgggtttc cgttatttcg tgctgcccca atgggccgct gatttcggcg cttccttccg
       61 ttccgccgat gccaagaacg gcgccctgca gtctgtcgat ttccacacgg gcctttcctt
      121 cttcgtcaac tcgcccttca agaacgcgga caagatggtc tgggcgggag gcggtgtgac
      181 catcatgcag ccggtcacca tcacggcgac cgaccaggga gcccagtccc tatccgacat
      241 tgcccgcgat gagtacggag acccggacct ttacccgctc atcatcgacg ccaactttgc
      301 cgataaggat aagcctctca tcctgccgcc cggcatccag ctcatcatcc cccaacaccc
      361 gaccggggaa atgatggccg ccgcccatgt gaaggccaag acgcccgttt ataaaaaagc
      421 ggcggatacc ttccgcatgc ccgaattgga caacctgcaa taccgactgg agcccgccca
      481 aaataatcag gagaccatgc tgggtgggca acggagcgag ctcaccttcc gggctgattc
      541 actgtgggat atcgcggccc gcccggacgt ctacggcgac cccgaacttt accccctgct
      601 ggtggacgcc aacttcagcc gcctttcgga cccgctcctg ctgaagcccg gggcccacct
      661 ggtcatcccg cgacacccca gcgaacggga aaagcgcatg gctcgcgtga aagcctggac
      721 cgtcgattac atccgctggc gtggcgtgga cgtgaccgag ggtcaatacc gggagtggcg
      781 caaggaaagg ggactgccgc cggtggatga tgtgaccggc gaggtgcttc agccccaacc
      841 ggcggatgaa ggcaactgat ccaaagtcca cccctttcgg gggcggaaac cccgtatcaa
      901 attcgaccga ggtaccggat gcgcatccgc tccaaaagga tcttaaggcc tcttggcctg
      961 atgatggttt ggctttattt ttgttccccg ttcttgccct tgtggacggc gggggccgcg
     1021 gcccagacgc caagcgggga atatcccaag ctcaccatcg ggcagggcga cattttggac
     1081 atccaggtct atggcgagaa cggcgggaac atccaaaatt ccgggattgg cgagaccgcc
     1141 ttgcctgtgg
//
LOCUS       DASTJJ010000671         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1560522, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000671 DASTJJ010000000
VERSION     DASTJJ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560522"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1155
                     /locus_tag="VFR02_08220"
     CDS             <1..>1155
                     /locus_tag="VFR02_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body FlgE domain-containing
                     protein"
                     /protein_id="HET9870465.1"
                     /translation="IQGYNATLEYTQTVINTFSSVPGRPAVVTQAAFQINNRDPAAIS
                     AIHIDPNLTLPPKATTQVNFTGNLDSFQQADQPGGILDLNPGGNPILPFSAGIDDFGF
                     NMNGAKMQLQGLPNGGYAFQQVGDLTQNTPADPYGLEYLGIDLNEVQADAGNYAWEQQ
                     PPVPPATQTAETIYDSLGNPHELTVLFYQVNDLGEAGINNNTAAGQSQACYAWYAFDT
                     TGGQKPTTANLVGGTGISEGAFTPPLFNTSYDRGLRGLYIGDFIFFNTDGSLANTGGV
                     SGFPPAFNPVPDVPRVYIQATNLLGIISPHPTEGAELMAVDLNFGTVGVLGQGKRDGL
                     FSDAEGSYQVVNGVNTYVPQHTAHAASQDGYADGTLESLSVDRTGTLQGT"
BASE COUNT          221 a          431 c          340 g          163 t
ORIGIN      
        1 tcatccaggg atacaacgcc accctggagt acacccaaac ggtcatcaac accttttcgt
       61 cggtgccggg ccggcccgcg gtggtgaccc aggccgcgtt ccagatcaac aaccgggatc
      121 cggcggccat ctcggccatc cacatcgacc ccaacctgac cctcccgccc aaggccacca
      181 cccaggtcaa cttcaccggg aacctggatt cgttccagca ggccgaccag cccggcggca
      241 tcctggacct gaacccgggc ggcaacccca tcctgccctt ctcggccggc atcgacgatt
      301 tcggcttcaa catgaacggc gccaagatgc agctgcaggg cctgcccaac gggggctacg
      361 ccttccagca ggtgggcgac ctgacccaga acacgccggc cgacccttac ggcctggaat
      421 acctgggcat cgacctgaac gaagtgcagg cggacgccgg caactacgcg tgggaacagc
      481 agccgcccgt gccgcccgcc acccagaccg ccgagaccat ttatgattcc ctgggcaacc
      541 cccatgagct gacggtgctg ttctaccagg tgaacgacct gggcgaggcc ggcatcaaca
      601 acaacacggc ggccggacag agccaggcct gctacgcctg gtacgccttc gacaccaccg
      661 gcggccagaa acccaccacg gccaacctgg tgggcggcac gggcatctcg gaaggggcct
      721 tcacgccgcc cctgttcaac accagctatg accggggcct gcggggcctc tacatcggcg
      781 atttcatctt cttcaacacg gacggttccc tggccaatac ggggggcgtg tccgggttcc
      841 cgccggcctt caaccccgtg cccgacgtgc cgcgggtcta tatccaggcc accaacctgc
      901 tgggcatcat ctcgccccat cccaccgagg gggccgagct catggccgtc gacctgaact
      961 tcggcaccgt gggggtcctg ggccagggca agcgcgacgg cctcttcagc gacgccgagg
     1021 ggagctacca ggtagtgaac ggggtgaaca cctatgtgcc ccagcacacc gcccacgccg
     1081 ccagccagga tggttacgcc gacgggaccc tggaaagcct ctcggtggac cgcacgggca
     1141 ccctgcaggg gacct
//
LOCUS       DASTJJ010000672         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_950223, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000672 DASTJJ010000000
VERSION     DASTJJ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950223"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFR02_08225"
     CDS             complement(<1..424)
                     /locus_tag="VFR02_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9870466.1"
                     /translation="MRRAALSLAAAVILAAGGAQAAGYSIDPQHSSLNFKVKHLSISY
                     VMGKFSKFSGTFSFDPAHAEKSKVSVTIDPASVDTGETARDNDLRSDHFFEVATYPTA
                     TFTSTKVTPLVDGKFQIMGKLTLHGVTKPVTLDAVYDGA"
     gene            642..944
                     /locus_tag="VFR02_08230"
     CDS             642..944
                     /locus_tag="VFR02_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HET9870467.1"
                     /translation="MKRNVFNDRSKLVPTALASLVAFGAAGVASQALAKDWTKMVKTE
                     KCYGVAKAGKNDCATKSHACAGMSKVDNDPNDWVFLPVGVCDKLTHAMDKSDKKAM"
     gene            947..>1071
                     /locus_tag="VFR02_08235"
     CDS             947..>1071
                     /locus_tag="VFR02_08235"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HET9870468.1"
                     /translation="MPSKIQGAGLGLRSRHLARILETGPDVPWFEVLADNYMNPG"
BASE COUNT          210 a          323 c          353 g          185 t
ORIGIN      
        1 ccgcgccgtc atagacggcg tccagggtga ccggtttggt gacgccatgc agggtcagct
       61 tgcccatgat ctggaacttc ccgtccacca ggggggtcac tttcgtgctg gtgaaggtgg
      121 cggtggggta ggtggccacc tcgaagaagt ggtcgctgcg caggtcattg tcccgggcgg
      181 tctcccccgt gtccacgctg gccgggtcga tggtcaccga gaccttcgac ttttcggcgt
      241 gggccgggtc gaaggaaaag gtccccgaga acttggagaa cttgcccatc acgtagctga
      301 tgctcaggtg cttgaccttg aaattgaggc tgctgtgctg gggatcgatg ctgtagccgg
      361 cggcttgggc gccgcccgcc gccaggatca ccgccgccgc cagggataac gctgcgcgcc
      421 gcataaggac cttccttccg aaccaaggga tagaggctca cgaccgccca tagatacggg
      481 gcccgccccc ggcgggttac cgccggccag gggaaaaagg acccggaaaa accgtcttcc
      541 cggaaaaaac cggaaggggt aactttttaa aagcccccgg gtatattgga ccaggcagcg
      601 ggtcccaaac ccaggcctga tcaaactcaa ggagtgtgtt catgaaaagg aatgttttca
      661 acgaccgttc gaagctggtc ccgacggccc tggccagctt ggtggccttc ggcgcggcgg
      721 gcgtggcctc gcaggccttg gccaaggatt ggaccaagat ggtcaagacc gagaagtgct
      781 acggcgtggc caaggcgggc aagaacgatt gcgccaccaa gtcgcacgcc tgcgcgggca
      841 tgtccaaggt ggacaacgac cccaacgact gggtcttcct gccggtgggc gtttgcgaca
      901 agctgaccca cgccatggac aagtccgaca agaaggcgat gtaagcatgc cttccaagat
      961 ccagggggcg gggctcgggc tccgctcccg gcatcttgcc cgcatcctcg agaccgggcc
     1021 cgacgtgccc tggttcgagg tcttggccga caactatatg aacccgggcg a
//
LOCUS       DASTJJ010000673         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_950239, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000673 DASTJJ010000000
VERSION     DASTJJ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950239"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFR02_08240"
     CDS             <1..1021
                     /locus_tag="VFR02_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870469.1"
                     /translation="TRTSTPTATPTITMTPSSTPTPAPVCTGSLSDPKLDLQVGCYQR
                     DNQQERLHVKIVNWSSSPVTLAHLGIKMWVYESQLVNMQQWVQMGQLFQADGSVADNV
                     SNTGFTASVAWLSQPCTVDATHKADQVVLFSPAGSSQAIPANGGHWDGGFDLALGRNN
                     PQMDDNWADDYSKTGACSGVLAEDGHFGLYYDGDLLQEWTSASALDANTGAAPCCGGS
                     GGFGSQMVHIGDGSTATPTPVVLVPILGKVEAAPNMTDGRAPVRFLFNLGQKARVTLA
                     LYAITGEEVYRTQVEGGAGDNSLVWKVDNQAGQTLASGLYFYILRVENGTTDLTRSGK
                     IAVIK"
BASE COUNT          202 a          338 c          356 g          187 t
ORIGIN      
        1 tacccggacg tcgacgccga cggccacccc gacaataacg atgaccccca gttcgacgcc
       61 gacccccgcc ccggtctgta ccgggtcctt gtccgacccc aagctggacc tgcaggtcgg
      121 ctgttaccaa cgggacaacc aacaggaacg gctccacgtc aagatcgtca attggtcctc
      181 atcgccggtc accctggccc acctgggcat caagatgtgg gtctacgaat cccagttggt
      241 gaacatgcag caatgggtac agatggggca gttgttccag gccgacggtt cggtcgcgga
      301 caatgtgtcc aatacggggt tcaccgcttc ggtcgcctgg ctttcccagc cctgcacggt
      361 ggacgccacg cataaggccg accaggtcgt gctcttctcg cccgccggca gctcacaggc
      421 gatccccgcc aacggcggtc attgggacgg cggcttcgac ctggcccttg ggcggaacaa
      481 cccccaaatg gacgacaact gggccgacga ctacagcaag acgggcgctt gttccggggt
      541 cttggcggag gacggccatt tcgggcttta ttacgacggg gatctgctgc aggaatggac
      601 cagcgcgtcg gccttggacg ccaatacggg ggcggcgcct tgttgcggcg gctcgggagg
      661 gttcgggtcc cagatggtcc atatcgggga cggctcgacg gcgacgccga cgcccgtggt
      721 cttggtcccg attttaggga aggtggaggc cgcgcccaac atgaccgacg gccgcgctcc
      781 ggtgcggttc ttgttcaact tgggccaaaa ggcccgggtc accttggccc tctacgccat
      841 cacgggcgaa gaagtctacc ggacccaggt cgagggaggg gcgggggaca acagcctggt
      901 ctggaaggtg gacaaccagg cgggccaaac cctggccagc ggcctttatt tctatatcct
      961 gcgggtggag aacggcacga cggacctgac ccgatcgggc aagatcgcgg tcatcaagta
     1021 ggatggtagg aagatgtttt gatgggatgc tgcggccgtc cgaaggggtc cggccaaccc
     1081 tca
//
LOCUS       DASTJJ010000674         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1193529, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000674 DASTJJ010000000
VERSION     DASTJJ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193529"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1023)
                     /locus_tag="VFR02_08245"
     CDS             complement(<1..>1023)
                     /locus_tag="VFR02_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870470.1"
                     /translation="LKLWAYDTGISNWVLDSFFMGSLFNGAGGFQANAAGSWGLTSIP
                     FPLCDADPAHRANWELDVTNNSPQFIPANGGYWLDGNFSIHPAGFNPNFNPGLAAWYS
                     GLPSGQASCSDASGSAGDYFDDLHFAIYYRGTLVAERGGLDPQTGRIPCAPNCTVTAS
                     PSPTLSRTATFTPTWTGTSTFTRTKTPTVTVTLTPTPLLAPTLSPTRTSTVLLTATST
                     RTFTATRTFTATRTFTATWTNTLTPTVTLSPTITLSPTITDTWTVTRTPTQTWTFTPT
                     NTFTPTHTPSVTPTPTPGCCVMQDMTTAGSVFLPAGLVMDDANQRFYVASARGPITGT
                     QPDPFI"
BASE COUNT          173 a          270 c          402 g          178 t
ORIGIN      
        1 cgatgaaggg atcgggttgg gtccccgtga tggggccgcg ggcgctggcc acgtagaacc
       61 gctggttggc gtcatccatc accagtccgg ccggcaggaa aacgctcccg gcggtggtca
      121 tgtcctgcat gacgcagcag ccgggggtcg gagtcggggt cacggacggc gtatgggtcg
      181 gggtaaaggt gttcgtcggc gtgaaggtcc aggtctgggt gggcgtgcgg gtcacggtcc
      241 acgtatcggt gatcgtgggt gaaagcgtga tggtgggcga cagggtcacc gtgggggtga
      301 gcgtattggt ccatgtcgcg gtaaaggtcc tcgttgcggt gaaagtacgg gtcgcggtaa
      361 aggtccgtgt actggtggcc gtcaggagga ccgtggacgt gcgggtcggc gacagggtcg
      421 gtgcgaggag gggggtgggg gtcaaagtga ccgtgaccgt gggggtcttg gtgcgggtga
      481 aggtcgacgt cccggtccag gtcggcgtaa aggtggcggt ccggctgagc gtcggactgg
      541 ggctggccgt gaccgtgcag ttgggggcgc aggggatccg cccggtctgg ggatccaggc
      601 ctccccgctc ggcgacgagg gtgccccggt aataaatggc gaaatggagg tcgtcgaaat
      661 agtcccccgc cgacccgctg gcgtccgagc aggaagcctg ccccgacgga agcccgctgt
      721 accaggccgc cagaccgggg ttgaagttgg ggttgaaccc cgcggggtgg atgctgaagt
      781 taccgtcaag ccaatatccg ccgttcgccg gtatgaactg cgggctgttg ttggtgacgt
      841 ccaattccca gttcgcccga tgggccggat ccgcgtcgca caagggaaaa gggatcgagg
      901 tcaggcccca ggatcccgcc gcgttggcct ggaaaccccc ggctccgttg aaaaggctcc
      961 ccatgaagaa ggaatccagg acccaattcg agatcccggt gtcgtaagcc cacaacttga
     1021 ggg
//
LOCUS       DASTJJ010000675         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1193873, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000675 DASTJJ010000000
VERSION     DASTJJ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193873"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFR02_08250"
     CDS             complement(<1..756)
                     /locus_tag="VFR02_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HET9870471.1"
                     /translation="MNRFLWAACLFLSPAALWAQPVVPTPAALAAPTVTAANGPKLYC
                     PYPKFDFHNVDEGPDIVHLFHFRNTGKSTLVISGVHTSCGCTAAVEDKKEVPPGGLGT
                     IKATFHTQGRPGHSTKIITVDTNDPVNPSFQMQLDMTVVREVDVQPERSYFYGLKHNT
                     SQTNQVTIVGKPHHYLHVLSAQPASGKVTVQMTPYVDKAAHRYGATLAITAPATLPIG
                     EFSDNIDVKTDDPKKPDLQVPIQGEVVGRVQYSP"
     gene            complement(963..>1433)
                     /locus_tag="VFR02_08255"
     CDS             complement(963..>1433)
                     /locus_tag="VFR02_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870472.1"
                     /translation="LWPRALFLPLAFLPFRRGLFREARWARWGSLAAFLLLSAATRVW
                     LHFQWNGLEFYDWFLGKHFAVFGVFVLLGWAAFPVRGTQRSVPLGVGLLALGFLLLAA
                     QPVGRTPGPAGLGWLLIWGAGFGFESLRRDLLDASWHGRAVWAAAGLAAFGGVF"
BASE COUNT          288 a          462 c          471 g          212 t
ORIGIN      
        1 ggggctgtat tggacccggc ccaccacctc gccctggatg ggcacctgca ggtcgggctt
       61 tttggggtcg tcggtcttca cgtcgatgtt gtccgagaat tcaccgatgg gcagggtggc
      121 cggcgccgtg atggccagcg tggccccgta gcggtgggcg gccttgtcga cgtagggcgt
      181 catctgcacc gtcaccttcc cggaggcggg ctgggcggaa agcacgtgca aataatggtg
      241 gggcttgccc acgatggtga cctggttggt ctggctggtg ttgtgcttga gcccgtaaaa
      301 gtaggaccgc tcgggctgca cgtccacttc ccgcaccacg gtcatgtcca gctgcatctg
      361 gaagctgggg ttcaccgggt cgttggtgtc caccgtgatg atcttggtgg aatggcccgg
      421 ccggccctgg gtgtggaagg tggccttgat ggtgcccagg ccgcccggcg gcacttcttt
      481 cttgtcctcg acggccgccg tgcagccgca ggacgtgtgc acccccgaga tgaccagggt
      541 ggacttgccg gtgttgcgga agtggaacag gtggacgatg tccgggccct cgtccacgtt
      601 atggaagtcg aacttggggt agggacagta gagcttgggc ccattggccg ccgtcaccgt
      661 gggggccgcc aaggcggccg gggtggggac caccggctgg gcccacaggg ccgccggaga
      721 aaggaatagg caagccgccc agaggaaccg gttcataacc acctcgccgt caaaatttat
      781 cgctcgatcc gccccaccgg ccgttcgatc cattcgccga cgggatccac agcaccggca
      841 ggagtcacgg catcagcggg attcgctgct tttcgcgggg aaaaaacggg ataatccgga
      901 gtttaccata atcccttttt gggatgcagc aaaaaagggc cgcggcccgc ccgcgcggtc
      961 cgtcaaaaaa cgcccccaaa agccgccagt cccgccgcgg cccagaccgc ccggccgtgc
     1021 cagctggcgt ccaacaggtc ccggcgcaac gactcaaaac cgaaacccgc gccccagatc
     1081 aacagccaac ccagccccgc cgggccgggg gtccgtccaa cgggctgagc cgccagcaaa
     1141 aggaaaccca aggccaagag gccgaccccc aagggcaccg accgctgggt gcctcggacc
     1201 gggaacgccg cccatcccaa caagacaaag acgccgaaga cggcaaaatg tttccccaaa
     1261 aaccagtcat aaaattccaa cccgttccat tgaaaatgga gccagacccg ggtcgcggcc
     1321 gacaaaagaa ggaacgccgc caaggagccc caccgggccc accgggcctc ccggaacagg
     1381 ccccggcgga aaggcaggaa agccaaggga aggaacaggg cccggggcca gag
//
LOCUS       DASTJJ010000676         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_338489, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000676 DASTJJ010000000
VERSION     DASTJJ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338489"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFR02_08260"
     CDS             complement(<1..759)
                     /locus_tag="VFR02_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET9870473.1"
                     /translation="MDVLTLSRAQFGFTVAFHYIFPPLSIGLGLILVTMESLYYFTQR
                     TLFLEMAKFWTRIFGLIFAIGVASGIVMEFQFGTNWAAYSRFVGDVFGSALAAEGIFA
                     FFLESGFLALLLFGWDRIGRGVHLFATFMVALGAHFSAVWIVVANSWQQTPSGYHLVA
                     QADGVTRAEVTDFWQVILSPSTLDRLWHVYMGAWQAGAFAVLSVSAYYLLKKKNKEFA
                     QASLKIALVVAAAASLLQLLSGHDSAVGVSRTQPA"
     gene            complement(829..1254)
                     /locus_tag="VFR02_08265"
     CDS             complement(829..1254)
                     /locus_tag="VFR02_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870474.1"
                     /translation="MVVFTFLIAFCLALAVLAGGLVLLGAQKGPWAKTAGFILAIGGG
                     LALACAGYHGLKYYFVGGFNGPFPGARASMSAGQGMMGSGGMMPMMDTQGMGNMPMMN
                     GTGGPGPRGMSSGKMGGTDHKNMGMDGDDADPASGAHHH"
BASE COUNT          264 a          423 c          416 g          185 t
ORIGIN      
        1 ggcgggttgg gtccgggaca cccccaccgc gctgtcatgg ccggacaaca gctgcagcag
       61 cgaggcggcg gcggccacca ccagggcgat cttgagggaa gcctgggcga actccttgtt
      121 ctttttcttg agcaggtagt aggccgagac gctcaacacc gcgaaggcgc ccgcctgcca
      181 ggcgcccatg tagacgtgcc agaggcggtc cagggtggaa ggcgagagga tgacctgcca
      241 gaagtcggtg acctcggcgc gggtgacgcc gtcggcctgg gccaccaggt ggtaacccga
      301 gggggtctgc tgccaggagt tggccaccac gatccagacc gcgctgaaat gggcgcccaa
      361 ggccaccatg aaggtggcga agaggtgcac cccccggccg atgcggtccc agccgaagag
      421 cagcagggcc aggaagcccg actccaggaa gaaggcgaag atgccctcgg cggccaaggc
      481 gctgccgaaa acgtcgccca cgaagcggga ataggcggcc cagttggtgc cgaactggaa
      541 ttccatcacg atgcccgagg ccaccccgat ggcgaagatg agcccgaaga ttcgggtcca
      601 gaacttggcc atttccagga agagggtcct ttgggtgaaa taatagaggc tctccatcgt
      661 caccaggatg agccccaagc cgatgctcag cggggggaaa atatagtgga atgcaacggt
      721 gaaaccgaac tgcgccctgg atagggtgag gacgtccatg ccttgctcct tggttcttga
      781 tcgatcaggt gaagccggcc gccccggggg acggccggcc cgcgacgctc agtggtgatg
      841 ggccccggaa gccgggtcgg cgtcatcgcc gtccatcccc atgttcttat ggtccgtccc
      901 gcccatcttg ccggaggaca tgccccgcgg gcccggaccg ccggtcccgt tcatcatggg
      961 catgttcccc atcccttggg tgtccatcat gggcatcatc ccgcccgagc ccatcatgcc
     1021 ttggcccgcg gacatggaag cccgcgcccc cgggaagggg ccgttgaacc ccccgacgaa
     1081 atagtatttt aaaccatggt atccggcgca agccagcgcc aggcccccgc cgatcgccag
     1141 gatgaacccg gcggtcttgg cccaggggcc cttttgggcg cccaacaaca ccaagccccc
     1201 ggccaagacc gccaaggcca ggcaaaaagc gatcaaaaac gtgaaaacca ccatgacaca
     1261 cctcccccac gaaatgtccg gggccccg
//
LOCUS       DASTJJ010000677         1168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_826018, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000677 DASTJJ010000000
VERSION     DASTJJ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826018"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFR02_08270"
     CDS             complement(<1..520)
                     /locus_tag="VFR02_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870475.1"
                     /translation="MKLSVKFSAFVLTAAMLGFAGQADAQGWVLQLGGGVSEPLSKQL
                     YSNYSLGYNASMALGYKVGGDLVLSVVTQADSLGLKQLSDIDNGQLPYDSSHPTTKSA
                     SFDSLQVSGIAKLYLGGVAALRPYVFGGPGVAFNDYHIHTDFPGNGSSFQSDFDTLET
                     DFFATAGLGAELD"
     gene            623..>1168
                     /locus_tag="VFR02_08275"
     CDS             623..>1168
                     /locus_tag="VFR02_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9870476.1"
                     /translation="MRTRLLGNSDMAITPLGVGTWAIGGPDGNYNWGPQDDGDSIAAI
                     RRAVERGVNWIDTAPAYGRGHSEEVVGKALKGLSPRPYVFTKNSLVWGEERVVTNRLK
                     ADSVRKECEASLKRLGVEWLDLWQIHWPNPDADIEEGWAAMAKLRQEGKVRWIGVSNF
                     SPAQMRRAQTIAPVTSLQPPYS"
BASE COUNT          226 a          357 c          396 g          189 t
ORIGIN      
        1 ggtccaattc ggcgcccagg ccggccgtgg cgaagaagtc ggtctccagg gtgtcgaaat
       61 cgctctggaa gctcgagccg ttgcccggga agtcggtgtg gatgtggtaa tcgttgaagg
      121 ccacgccggg cccgccgaag acgtaggggc gcagggccgc cacgccgccc aagtagagct
      181 tggcgatccc gctgacctgg aggctgtcga agctggcgga cttggtggtg gggtggctgg
      241 aatcataggg gagttgccca ttgtcgatgt ccgacagctg cttgagcccc aaggaatcgg
      301 cctgggtgac gacggacagc accaagtcgc cgccgacctt gtaacccagg gccatggacg
      361 cgttgtagcc cagcgaataa ttgctgtaga gctgcttgct cagcggttcg gaaaccccgc
      421 cgcccagttg aagcacccac ccttgcgcgt cggcttggcc cgcgaaaccc aacatggcgg
      481 ccgtcaaaac gaaggccgaa aatttaacgg ataatttcat ggatccattc ccccttggag
      541 ttagattgaa gcttgggcta aggtatcaaa tcctcccgct ttaacaaccc ctcatatcca
      601 ggggagaccc gaaaggaact ttatgcgaac gcgcttgctg gggaactcgg acatggccat
      661 cacgcccctg ggggtgggca cctgggccat cggcgggccg gacggcaact acaactgggg
      721 gccccaggac gacggggatt ccatcgcggc catccgccgg gcggtggagc ggggcgtcaa
      781 ttggatcgac acggcgcccg cctacggccg gggccattcg gaggaagtgg tgggcaaggc
      841 cctgaagggc ctgtcgcccc ggccctacgt cttcaccaag aattccctgg tctgggggga
      901 ggagcgggtg gtgacgaacc gcctcaaagc cgactcggtg cggaaggagt gcgaggcgag
      961 cctcaagcgc ctgggcgtgg agtggctgga cctctggcag atccactggc ccaacccgga
     1021 cgccgacatc gaggaaggct gggccgccat ggccaagctg aggcaagaag gcaaggtgcg
     1081 ctggatcggg gtgtccaact tcagcccggc ccagatgcgc cgcgcccaga ccatcgcccc
     1141 cgtgacctcg ctgcagcccc cctattcg
//
LOCUS       DASTJJ010000678         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1071974, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000678 DASTJJ010000000
VERSION     DASTJJ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071974"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFR02_08280"
     CDS             <1..781
                     /locus_tag="VFR02_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870477.1"
                     /translation="GNPPPNITSNDMLEGVVESDAQVHQSVIEEFLGSVSRSCRKELE
                     LMRDQGPEEILSRFLGGPGGKFYCQKFKKSVLRNLPDIWVMPGKSANQSRAQQRKDIE
                     LMLPIVLQQAGTPDQQKIIIQRAIETMMWGDEEGLVGTLEEQKNCVLDLIAELQDNAE
                     NPDFKINIMPWHDMKVWMNEIERVLLNKTEFDTWPEISKQRLIDLYEQVVQNLSKQMA
                     PQGGPSQTPQLPENKPPVPGMPPLPGAPPIPGMGQPPGLPR"
     gene            791..1084
                     /locus_tag="VFR02_08285"
     CDS             791..1084
                     /locus_tag="VFR02_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870478.1"
                     /translation="MALITPPRTAPQGSIWGHIADAFLGKSRDKSKEDPIEFHQRREG
                     EELHDIGMEDGKPIEKAPGTTSEITHPVLKNIMAQLSMGDAKRGGKFGGMRRG"
     gene            1147..2322
                     /locus_tag="VFR02_08290"
     CDS             1147..2322
                     /locus_tag="VFR02_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870479.1"
                     /translation="MESALTAPPTPAQPEGTAPSPARTGPLSLGDKARAAADAAKKAP
                     EPAKPKGESAPPAPAQDAPPEGAQEDESNATPEGEVDKVTWNGKEASLQDILEDGTFE
                     IFANGKTHSVEGFQKLADLASMGLDSANRNRLAKEGLVKAQDIVRELEGERDKAVKAL
                     KDVDGQVDSKVNAILDVLYKNVSKGFNADGTRFETNEDQQKALAQVRAMKTAFAGTPE
                     NPGPVSAADIEKMVQERLDKRFAEESGKQDQARQEAENNRLISDVGQRLTEATKSDGA
                     YFTKPDGKLNSTLFSTYKRELISRCQEEWNAQGQPRDLESISQVIREVRSLLIEDLKI
                     SDPGRKPEPKPAPPVLKPASGAGTPRSDKESRPHFRSPAEAMRYYADQAKKRLASGG"
     gene            2326..>2625
                     /locus_tag="VFR02_08295"
     CDS             2326..>2625
                     /locus_tag="VFR02_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870480.1"
                     /translation="MSAYTNIQYLLKNGYLNNEIIQQFAEDTVLYQVFEKDNKWVKAG
                     AGQGQGAPLTYGMAWPIETQRNNSVGTFGVSSPNLLPGSTRSGVNAYTNLLASHAS"
BASE COUNT          643 a          793 c          783 g          406 t
ORIGIN      
        1 ggggaatcct ccccccaaca tcacctccaa tgacatgctg gaaggggtcg tggagagcga
       61 cgcccaagtc caccaaagcg tcatcgagga attcctcggt tcggtttccc gttcctgccg
      121 caaggagctc gaactcatgc gggaccaggg gccggaggaa atcctgagcc gctttctggg
      181 gggaccgggt ggcaaattct actgccagaa gttcaagaaa tccgtcctgc gcaacctgcc
      241 ggacatctgg gtcatgcccg gaaaaagcgc caaccagtcc cgggcccaac aacgcaagga
      301 catcgaactc atgctgccga tcgtccttca gcaggccggt accccggacc agcaaaagat
      361 catcatccaa cgggccattg aaaccatgat gtggggtgat gaggagggcc tggtcgggac
      421 cctcgaggag cagaagaact gtgtgctgga cttgatcgcc gaacttcagg acaatgcgga
      481 aaacccggac ttcaagatca atatcatgcc ttggcacgac atgaaggtct ggatgaacga
      541 gatcgagcgg gtgctcctca acaagaccga attcgacacc tggcccgaga taagcaaaca
      601 gcgtctcatt gacctttacg aacaggtagt gcagaatctg tccaagcaga tggctcccca
      661 aggggggcca tcccagactc cccaacttcc ggaaaataaa cctccggtcc cggggatgcc
      721 tcccttgccg ggagcgccgc ccattcccgg catgggccaa ccccccgggc taccccgtta
      781 aggagatacc atggcactta tcacaccccc cagaaccgcc ccgcaaggct cgatctgggg
      841 gcatatcgcc gacgcttttc tcgggaaatc cagggataag tccaaggaag accccattga
      901 attccaccag cgccgggaag gggaagaact ccatgacatc ggcatggaag atggcaaacc
      961 catcgaaaag gcccccggaa cgacttccga gatcacccat cccgtcctga agaacatcat
     1021 ggcccaactc tccatggggg acgcgaaacg agggggaaag ttcggcggta tgaggagggg
     1081 ttgacgaaaa aggttgtctt tgacgtgtcc gttcgtgaca attcaatcga ctaggaggag
     1141 caccgcatgg aatccgcact gacggcgccc ccgactccgg cacagccgga agggaccgca
     1201 ccgagtccgg caagaactgg accactttct ttgggcgata aggcccgggc ggctgctgat
     1261 gccgctaaaa aggctccaga gcctgcgaaa cccaagggcg aatccgctcc ccccgctccg
     1321 gcccaagatg ccccgccgga aggcgcccag gaagacgagt cgaacgcaac acccgaaggt
     1381 gaagtcgaca aggtgacctg gaacgggaag gaagcttccc tccaagacat cctggaagac
     1441 ggtaccttcg agatcttcgc caacggaaag acccattcgg tcgaggggtt ccagaagctc
     1501 gcggacctcg cctccatggg gctggattcg gccaaccgga accggctggc gaaggaagga
     1561 ttggtcaagg cccaggatat cgtgcgcgaa ctcgaagggg aacgcgacaa agccgtcaag
     1621 gccttgaagg atgtggatgg ccaggtcgac tcaaaggtca acgccatcct ggatgtgctg
     1681 tacaagaacg tgtccaaggg cttcaatgcc gacgggacgc ggtttgagac gaatgaagac
     1741 cagcagaaag ccctggcgca agtcagggcc atgaagaccg ctttcgcggg gacccctgag
     1801 aacccggggc ccgtttcggc ggcggacatc gagaagatgg tccaggaaag gttggacaaa
     1861 cggttcgcgg aggaaagcgg caagcaggat caggcgcggc aggaagccga gaacaaccgc
     1921 ctgatctcgg atgtagggca gcgattgacg gaggccacga agtccgatgg ggcctatttc
     1981 acgaagcctg acgggaagct caattcgacg cttttttcga cgtacaagcg cgaactgatc
     2041 tcccgctgcc aggaagagtg gaacgcccag ggacagccga gggaccttga gtcgatctcc
     2101 caagtcatcc gggaagttcg ctcgctgctc atcgaagact tgaagatctc ggacccgggc
     2161 agaaagcccg agccgaagcc tgcgcccccg gtactcaaac cggcttccgg cgcggggacg
     2221 ccgcgttccg acaaggaatc gcgcccccac ttccggagcc cggcagaagc catgcggtac
     2281 tacgcggacc aggcaaaaaa aaggcttgcc tccggaggct aagaaatgtc cgcttatacc
     2341 aacatccagt acctgctcaa gaacgggtac ctgaacaacg agatcatcca gcagttcgcc
     2401 gaggacaccg tgctttacca ggtgttcgag aaggacaaca agtgggtgaa ggccggtgcg
     2461 ggtcagggcc agggcgcccc cctcacttac ggcatggcgt ggcccattga gacccagcgc
     2521 aacaactcgg tgggtacctt cggggtctcc tcacccaacc tgctgcccgg cagcacccgc
     2581 agcggtgtga acgcctacac gaacctcctg gcgtcgcacg cttcg
//
LOCUS       DASTJJ010000679         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_582265, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000679 DASTJJ010000000
VERSION     DASTJJ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582265"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2541)
                     /locus_tag="VFR02_08300"
     CDS             complement(<1..>2541)
                     /locus_tag="VFR02_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HET9870481.1"
                     /translation="RSAGTVEFVYDPGRDEFYFLEVNTRLQVEHGVTEAVTGIDLVEW
                     MVRLAAGQAIPLDRLPEPQGAAFEARLYAEDPARGFQPSPGTLTEVSFPADVRVDGWV
                     EAGTEVTPHYDALLAKLIVWGPDRAQALAKLRQALDATRLAGLDTNLDYLRQVAGSAF
                     LERGELSTAALAGFAYRPRALEVLRPGTYTTVQDYPGRVGYWDVGVPPSGPMDDYAFR
                     LANRAVGNPSSAAGLECTLEGPRIRFHQDAVAALTGAPAPATLDGRPLGFWEPFEVKA
                     GQVLDLGRAQAGCRTYLAVRGGLDLPLYLGSRSTFVLGGFGGLGGRPLREGDWIPLPR
                     DREGLEAPQAVPGGLVPPYPAEWEIGALDGPHGAPDFFTPRSVEMIFGTAWEVHYNSN
                     RLGIRLKGPKPAWARRDGGEAGLHPSNIHDTEYAVGSLNFTGDMPVILGRDGPSLGGF
                     VCPLTIAKAERWKMGQLKPGDRVRFKRLSFEQALAAQRAQDRAVASLAPARAQAARAP
                     RRRPGGLGGLLAQLPARGSHPAVAYRQAGDECVLIEYGPNRLDLRLRLRVHLLMEALR
                     REPLRAIRELSPGVRSLQVRYDSGVLPQAALLKALLKAERALPSVDGLRVPTRVVHLP
                     MAFEDSATLEAVTRYRRSVRDKAPWLPRNAEFIRRINGLKSVAQVRRILFEARYLVLG
                     LGDVYLGAPCAVPLDPRHRLLTSKYNPARTYTAEGTVGIGGVYMCIYGMDSPGGYQLV
                     GRTLPIWDRFPRAGGGHWLLRFFDQVRFYPVSEAELTVQREAFREGRAALRIEEEVFD
                     LDGYEAFLRRNRPGIEAFRKRQKAAFAREVARWRSSSGVEAPGEPPAPR"
BASE COUNT          382 a          935 c          904 g          320 t
ORIGIN      
        1 cgcggcgcgg gcggttcccc gggggcttcc acgccgctcg acgagcgcca ccgggccact
       61 tcccgcgcga aggccgcctt ttgtcgcttc cggaaggcct cgatgccggg gcggttgcgc
      121 cgcaggaagg cctcgtagcc gtccaggtcg aagacctcct cctcgatgcg cagggcggcc
      181 cgcccctcgc ggaaagcctc ccgctggacc gtcagttcgg cctcgctcac cgggtaaaag
      241 cgcacctggt cgaagaagcg cagcagccaa tggccgccgc cggcccgggg aaagcggtcc
      301 caaatgggca gggtgcgccc caccagttgg tagccgcccg gcgagtccat gccgtagatg
      361 cacatgtaga caccgccgat gcccaccgtg ccttccgccg tgtaggtgcg cgccgggttg
      421 tatttggagg tgaggaggcg gtggcggggg tccagcggca cggcgcaggg cgcccccagg
      481 tagacgtcgc ccagccccag caccaggtag cgggcctcga agaggatgcg gcgcacctgg
      541 gccacgctct tgaggccgtt gatgcgccgg atgaactcgg cgttgcgggg cagccagggc
      601 gccttgtccc gcacggaccg gcggtagcgg gtcaccgcct ccagggtggc cgagtcctcg
      661 aaggccatgg gcaggtgcac cacccgggtg ggcacgcgca ggccgtccac cgagggcagc
      721 gcccgttcgg ccttcaagag cgccttcaag agggcggcct ggggcaggac cccgctgtca
      781 tagcgcacct gcagggaccg cacgcccggg gaaagctccc ggatggcccg caggggttcc
      841 cggcgcaggg cctccatcaa caggtggacc cgcaggcgca gccgcaggtc cagccggttg
      901 ggcccgtatt cgatcagcac gcactcatcc cccgcctgcc ggtaggccac cgccggatgg
      961 gacccgcggg ccggcagctg ggccaggagc cccccgagac cgccggggcg ccggcgcggg
     1021 gcgcgcgcgg cttgggcgcg cgcgggcgcc agcgacgcca ccgcccggtc ctgggcccgc
     1081 tgggccgcca aggcctgctc gaagctgagc cgcttgaacc gcacccggtc ccccggcttg
     1141 agctgtccca tcttccagcg ctcggccttg gcgatcgtca gggggcagac gaaaccgccc
     1201 aggctgggcc cgtcccggcc caggatgacc ggcatgtcgc cggtgaaatt gagcgacccc
     1261 accgcgtact cggtgtcgtg gatgttggag ggatgaagcc ccgcctcccc cccgtcccgg
     1321 cgggcccagg cgggtttggg acccttcaag cggatgccca gccggttgga attgtagtgc
     1381 acttcccagg ccgtgccgaa gatcatctcc accgaacggg gcgtgaaaaa atccggcgcc
     1441 ccgtgggggc cgtccagcgc cccgatctcc cactcggccg gataaggggg gaccaggccc
     1501 ccggggaccg cttggggcgc ctccaagccc tcccgatccc ggggcaaggg gatccagtcg
     1561 ccttcccgca agggacgccc gcccaaaccg ccgaagccgc ccaacacgaa ggtggaccgg
     1621 ctgcccaggt acaggggcag gtccaacccg ccccggacgg ccaggtaggt ccggcatccc
     1681 gcttgggcgc ggcccaggtc caacacctgg cccgccttca cctcgaaggg ttcccagaag
     1741 cccaagggtc ttccatccag ggtggccgga gccggggcgc cggtcagggc ggccaccgcg
     1801 tcctggtgga agcggatccg ggggccctcc agggtgcatt ccaacccggc ggccgaggaa
     1861 gggttcccca ccgcgcggtt ggccaggcgg aaggcgtaat cgtccatggg ccccgatggc
     1921 ggcacgccca cgtcccaata gcccacccgc cccggatagt cctgcacggt ggtgtaggtg
     1981 ccgggccgca gcacttccaa cgcgcgggga cggtaggcga agcccgccag cgcggcggtg
     2041 gaaagctcgc cccgctcgag gaacgcggac ccggccacct gccgcaggta atccaggttg
     2101 gtgtccaggc ccgccaggcg cgtggcgtcc agcgcctggc ggagcttggc cagggcctgg
     2161 gcccggtccg gcccccacac gatgagcttg gccagcaggg cgtcgtaatg gggcgtgacc
     2221 tcggtccccg cctccaccca gccgtccacc cggacatccg ccgggaaaga gacctcggtc
     2281 agggtcccgg gcgagggctg gaacccgcgg gccgggtcct cggcgtaaag cctggcctca
     2341 aaggccgcgc cctggggctc gggaaggcgg tccaggggga tggcctgccc ggcggccagc
     2401 cgcaccatcc attccaccag gtcgatcccc gtcacggcct cggtgacgcc gtgttccacc
     2461 tgcaggcgcg tgttgacctc gaggaaatag aactcgtccc gcccggggtc gtacacgaac
     2521 tccacggtcc ccgccgaacg g
//
LOCUS       DASTJJ010000680         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1072383, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000680 DASTJJ010000000
VERSION     DASTJJ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072383"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3326)
                     /gene="rlmD"
                     /locus_tag="VFR02_08305"
     CDS             complement(<1..>3326)
                     /gene="rlmD"
                     /locus_tag="VFR02_08305"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET9870482.1"
                     /translation="QFVVDALSRIGKMDAVTVEPCLPSEASYGYRNKAMPVLSMREGH
                     FISGIYEPRSHKLVPYQQCAIQADALNGLIQKTLHKIDLAGLTPYQEKKHEGFLRHLA
                     VRQGAGTGEMLLAFVTRTQYPEERLHKPSLLPEPLADILPRLAQELMAEVPGLVGVLQ
                     NVNPSRTNIVFGPATEVLAGRDHYFEKLDGLTLKVSLKSFLQVNSAQAEVLHTVVREA
                     LGVPEDGRKKWGTVLDLYSGIGTLALAVAEKADYVVGAEEVAPAVEDARENAALNRKG
                     NLDFLEGDVAEMLLRLKAQGLAQIDAAILDPPRKGVLPEVLSRVTAFHPRRLVYVSCD
                     PTTLARDLALLAKHGYAVDWAQPLDMFPQTYHVETVVRLTRTAPLPQQDPAALKEGLE
                     PFRLPKGPEVSMNAQGASRGLGWWARALGEALGARLAGLFQKKEGFQWPLAEMAEAPS
                     EAAPVSSNPEALPADGTAGAPGTAALPAAMETADPAPSVPVLPDAGGPPRRRFFSREA
                     LAFPWSARTTGLALLTLFLLAGGGYWAAADLSPLIANGSFQSSSIPQFIPDTIAIMPT
                     RNFLRYEMVPFEVRIHPEDMPHFASMHAVVEVFHDGQPVSMVDGRTRLYLKKDPANAR
                     FIGNWPIPYNPVPGTYTAQIALTGPQWPDPKEFESAFTISPLKPSGLYPGYSALTMEG
                     GKHLINGAVPPVDGRGGDSAANAIGWAKFMGANTYCFLAGQTSVWDKLNPKDFPFNRG
                     DLALADRYGAAAHDAGIKFAAYMTTFRVVGDGWQQAADRYHFTLGYDPATDQVVPTDF
                     VSLEDPTRRQDILALLRQMDHDPNIDMIGLDYVRTGTGGYEFVDEFVRDLNVPGPADY
                     WSMSKDERIHWLARTVERRENKDVVALFEWWRAHKVAMTLKSILDEAKLSKPVFTFTL
                     GWDMGHQHGQDPAMFEDAGVNFNHIMLYQSDRKTIEQMNRQWPPYIARSNGMYALGEM
                     VDENWVQNSLDPPGPEELYDREAATFRAWHPVNAGLGMFWHDLYRILYGQKGYYSSME
                     WALAGAKSFSMLQQAEGTLPLEVQLSVPAAAPEGAKVPLSVEIHNHSVETLTGIVLHQ
                     IDTSKDYDTDLATVGPFDIP"
BASE COUNT          524 a         1070 c         1157 g          575 t
ORIGIN      
        1 cgggatgtcg aagggaccca ccgtggccag gtccgtgtcg tagtctttcg aggtgtcgat
       61 ctggtgcagc acgatccccg tcagggtctc caccgagtgg ttgtggatct ccacgctcag
      121 gggcactttg gcgccctcag gcgcggcggc gggcacgctc aactgcactt ccagcggcag
      181 cgtgccctcg gcctgttgga gcatggaaaa ggacttggcc ccggccagcg cccattccat
      241 ggagctatag tatcccttct ggccgtaaag gatgcggtac aggtcgtgcc agaacatgcc
      301 gagcccggcg ttcaccgggt gccaagcccg gaaggtcgcg gcctcccggt cataaagctc
      361 ctcggggccg ggggggtcga gggaattctg cacccaattc tcatccacca tctcgcccag
      421 cgcgtacatg ccgttgctgc gggcgatgta gggcggccat tggcggttca tctgctcgat
      481 ggtcttccgg tcgctctgat acagcatgat gtggttgaag ttgacccccg cgtcctcgaa
      541 catggcgggg tcctgcccgt gttggtggcc catgtcccaa cccagggtga aggtgaagac
      601 cggcttggag agcttggcct cgtccaggat gctcttcaag gtcatggcca ccttgtgggc
      661 ccgccaccac tcgaagagcg ccaccacgtc cttgttctcc cggcgctcga ccgtgcgggc
      721 cagccaatgg atgcgctcat ccttgctcat ggaccagtaa tcggccggcc ccggcacgtt
      781 caggtcccgc acgaactcgt ccacgaactc gtagccgccc gtgccggtcc gcacgtaatc
      841 caagccgatc atgtcgatgt tggggtcgtg gtccatctgc ctcaagaggg ccaggatgtc
      901 ctggcgccgg gtcgggtcct ccagggagac gaagtccgtg ggcaccacct ggtccgtggc
      961 ggggtcgtat cccagggtga agtggtagcg gtccgcggcc tgctgccagc cgtcccccac
     1021 cacccggaag gtggtcatgt aggcggcgaa tttgatcccc gcgtcatggg cggccgcgcc
     1081 gtaacggtcg gccagcgcca ggtcgccccg gttgaagggg aagtccttgg ggttcaactt
     1141 gtcccacacc gaggtctgcc cggccaggaa acaataggtg ttggcgccca tgaatttggc
     1201 ccatccgatg gcgttggccg ccgaatcccc gccccggccg tccaccggcg gcacggcccc
     1261 gttgatcagg tgcttgcccc cctccatggt gagcgccgag tagcccggat aaaggcccga
     1321 gggcttgagg ggcgagatgg tgaaggccga ttcgaattcc ttgggatcgg gccattgggg
     1381 cccggtgagg gcgatctggg ccgtataggt gccgggcacg ggattgtagg ggatgggcca
     1441 gttgccgatg aacctggcgt tggccgggtc tttcttcagg tacaaccggg tgcggccgtc
     1501 caccatggac acgggctggc cgtcatggaa gacctccacc acggcgtgca tggaggcgaa
     1561 atggggcatg tcctccgggt ggatgcgcac ctcgaagggc accatctcgt agcgcaggaa
     1621 attccgggtg ggcatgatcg cgatggtgtc ggggatgaat tgggggatcg aggaggactg
     1681 gaaggacccg ttggcgatga gcggcgacag gtccgccgcg gcccaataac cgccccccgc
     1741 caaaagaaaa agggtcagca gggccaggcc cgtcgtccgg gcgctccagg gaaaggccag
     1801 ggcttcccgc gagaaaaagc ggcgccgggg cggccccccc gcgtccggga gcaccgggac
     1861 ggatggggcc ggatcggccg tttccatggc ggccggcagg gcggccgtcc cgggagcccc
     1921 ggccgttccg tccgccggga gggcttcggg gttggacgaa acgggcgcgg cctccgacgg
     1981 ggcttcggcc atctccgcca gcggccactg gaatccttcc ttcttttgga agagcccggc
     2041 caagcgcgct cccagggcct cccccagggc ccgcgcccac caacccagcc cccgggaggc
     2101 gccttgggcg ttcatggaaa cctccgggcc tttgggcagg cggaaaggtt ccaaaccttc
     2161 cttcaaggcg gccggatcct gctggggcag gggcgcggtg cgggtcaggc ggaccaccgt
     2221 ctccacatgg taggtctggg ggaacatgtc caggggctgg gcccaatcca ccgcgtaacc
     2281 gtgcttggcc aacaacgcca ggtcgcgggc cagggtggtg gggtcgcagg atacgtagac
     2341 caagcggcgc gggtggaagg ccgtcacgcg cgacagcacc tcgggcagca cgcccttgcg
     2401 gggcgggtcc aggatggccg cgtcgatctg ggcgagcccc tgggccttga gcctcaagag
     2461 catttcggcc acgtcgcctt ccaggaaatc caggttcccc ttgcggttga gggcggcgtt
     2521 ctctcgggcg tcctccaccg cgggcgccac ttcctccgcg cccaccacgt agtcggcctt
     2581 ctccgccacg gccagcgcca gggtgccgat gcccgagtag aggtccagca cggtgcccca
     2641 ttttttgcgg ccgtcctcgg ggacccccag ggcctcccgc accaccgtgt gcagcacctc
     2701 ggcctgggcg gagttcacct gcaggaagga cttgagcgag accttcaggg tgagcccgtc
     2761 gagcttctcg aaataatggt cccgtcccgc caagacctcg gtggcggggc cgaagacgat
     2821 gttggtgcgg gacgggttga cgttctgcaa cacgcccacc aggcccggca cttcggccat
     2881 cagttcttgg gccaggcggg gcaggatgtc ggccaggggt tcgggtagga ggctgggttt
     2941 gtgcagccgc tcctccggat attgggtgcg ggtcacgaag gccaacagca tctcacccgt
     3001 gcccgcgccc tggcgtaccg ccaggtgccg caggaacccc tcgtgctttt tttcctggta
     3061 gggcgtcagc ccggccaggt cgatcttgtg gagcgtcttt tggatgagcc cgttcagggc
     3121 gtcggcctgg atggcgcatt gctggtaggg caccagtttg tgggaccggg gctcgtagat
     3181 gcccgagatg aaatggccct cccgcatgga cagcaccggc atggccttgt tgcggtagcc
     3241 gtaggaggcc tcggagggca ggcagggctc gaccgtgacc gcatccatct tgccgatgcg
     3301 cgacagcgcg tccaccacga actggc
//
LOCUS       DASTJJ010000681         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1561944, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000681 DASTJJ010000000
VERSION     DASTJJ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561944"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFR02_08310"
     CDS             complement(<1..974)
                     /locus_tag="VFR02_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9870483.1"
                     /translation="MPKDFKPFSIYDDDEEPKGPPAPPPASEDDLGLEIEDGPGPEYI
                     KAREDLLKQLGLDASSDAAKIPAAPTEPPSENLEQAPFLQGQADQSVEDRAQAIIRTR
                     RETGKIRDRRKVASIFDKPIEPDRPIRPGKSSWHPLGHRAQAFWFLFPALLLFCLFYL
                     YPMVKGFQISLTHYDPLGHSVPVGWGNYDKAFGDSFFWQTVVNAVAFTLASIVVGFWP
                     PIFLAILLNEITRGQGLLKVLYLLPFVIPVVPAANLWRWMFDDGFGVFNELLRALTGD
                     PHLTVGWLTDPRWALLSIVAMFVWKNMGWFMLIYYASLQNLPDELYEAA"
BASE COUNT          239 a          363 c          396 g          227 t
ORIGIN      
        1 gcggcctcat aaagctcgtc gggcaggttc tgcagcgagg cgtagtaaat gagcatgaac
       61 cagcccatgt tcttccagac gaacatggcc acgatggaaa gcagcgccca gcgggggtcg
      121 gtgagccagc ccaccgtgag gtgcgggtcc cccgtcaggg cgcgcagcag ctcattgaag
      181 accccgaacc cgtcgtcgaa catccagcgc cacaggttgg ccgccggcac caccgggatg
      241 acgaagggca gcaggtagag caccttcaac aggccctggc cccgggtgat ctcgttgagc
      301 aggatggcca ggaagatcgg cggccagaac cccaccacga tggaggccag ggtgaaggcc
      361 accgcgttga ccacggtctg ccaaaagaag gaatcgccga aggccttgtc gtagttgccc
      421 caccccaccg gcacgctgtg ccccaggggg tcgtagtggg tcaggctgat ctggaacccc
      481 ttcaccatgg ggtaaaggta gaagaggcag aagagcagca gcgccgggaa gaggaaccaa
      541 aaggcctggg cccggtggcc caagggatgc caggaggact tccccggccg gatgggccgg
      601 tcgggctcga tgggcttgtc gaagatggag gccaccttgc ggcggtcgcg gatcttgccg
      661 gtctcccgcc tcgtccggat gatggcctgg gcccggtcct ccacgctttg gtcggcctgg
      721 ccctgcagga aaggcgcctg ttccaggttc tccgagggcg gctcggtggg ggccgccggg
      781 atcttggccg cgtccgagga ggcgtccagg cccagctgtt tcagcaggtc ctcgcgggcc
      841 ttgatgtact cggggccggg gccgtcctcg atctccaggc ccaggtcgtc ctcggacgcg
      901 ggcggcggcg ccgggggccc cttgggctcc tcgtcgtcgt cgtagatgga aaaaggcttg
      961 aagtccttcg gcaagcttgg gctcctaatt catttcagga tcattctctt cacaaaacaa
     1021 agacaattcg gaacattgat agggactgat aaagcctgat ttatatatcc aaacatcaga
     1081 tttcatcaat ctttgtcatt gtttcaaaag ccctttctat ttgcctttca aaagacattg
     1141 ttccgatcca ttttcgagaa gatgtcgcgg tcggccttct tggcgtagtc cagcagcaac
     1201 tggcccggat cggcgttggg gtcca
//
LOCUS       DASTJJ010000682         1230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1561998, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000682 DASTJJ010000000
VERSION     DASTJJ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561998"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFR02_08315"
     CDS             complement(<1..961)
                     /locus_tag="VFR02_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET9870484.1"
                     /translation="MEYRTEHDSIGELKVPAEALWGVQTQRAVHNFPISGIKPHAVFV
                     DATVHIKRAAAVFHGQVGWLDKKKAAAIVAAADEVLKKGKWRDQFVVDVYQAGAGTSH
                     NMNVNEVLANRAIELLGGRRGDYKLVHPNDHVNMGQSTNDVIPTAIRLSGLMLVRDFL
                     PVLDKLSKTFQKKGQEFKKVFKSGRTHLQDAAPVTLGQEFRAYGECLKRHLARIQRAS
                     GDLQELGIGGSAVGSGLNTHKKYPAGMAKILSKETGLKLAPHPDLFEAMQSLAPAVSL
                     SNALRGLCLDLTRIANDFRLMTSGPTTGFGEILLPAMQPGSSIM"
     gene            complement(1063..1137)
                     /locus_tag="VFR02_08320"
     tRNA            complement(1063..1137)
                     /locus_tag="VFR02_08320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1103..1105),aa:Gly,seq:tcc)
BASE COUNT          205 a          369 c          412 g          244 t
ORIGIN      
        1 gcatgatgga agagcccggc tgcatggccg gcagcaggat ctcgccgaaa cccgtggtgg
       61 gcccgctggt catgagccgg aaatcgttcg cgatgcgggt caggtccagg cacaggcccc
      121 ggagggcgtt ggaaagcgac acggcggggg ccaggctctg catggcctcg aagaggtccg
      181 ggtggggggc gagcttgagc ccggtctcct tggaaaggat cttggccatc ccggccgggt
      241 atttcttgtg ggtgttcaag cccgacccca ccgccgatcc cccgatcccg agttcctgca
      301 ggtccccgga agcgcgctgg atgcgggcca ggtggcgctt caggcattcc ccataggccc
      361 ggaactcctg ccccagggtg acgggcgcgg cgtcctgcag gtgggtacgg cccgacttga
      421 agaccttctt gaattcttgg cccttcttct ggaacgtctt ggatagttta tccaggaccg
      481 gcaaaaagtc ccgcaccagc atcaggcccg acaggcggat ggccgtgggg atgacgtcgt
      541 tggtggattg gcccatgttg acgtggtcgt tggggtggac caacttgtag tcgccccgcc
      601 gcccccccaa cagctcgatg gcccggttgg ccagcacctc gttgacattc atgttgtggc
      661 tggtgccggc gcccgcttgg tacacatcca ccacgaattg gtcgcgccac ttcccttttt
      721 tcaggacctc gtcggccgcg gccacgatgg cggccgcctt cttcttgtcc agccagccca
      781 cctggccgtg gaaaacggcg gccgcgcgtt tgatgtggac cgtggcgtcc acgaagaccg
      841 cgtggggctt gatcccgctg atggggaaat tgtgcaccgc ccgctgggtc tggacgcccc
      901 acagggcctc cgcggggacc tttaattctc cgatcgagtc atgctccgta cggtattcca
      961 acggttcctc caggacttgg tctaggtttg gattcggggt tcggaaataa gggtgaaggt
     1021 tgagggatcg ggcttggtcc gggcccgggc cgggaacgag tttggagcgg gagaccggat
     1081 tcgaaccggc gacgtccacc ttggaagggt ggcattctac cactgaatta ctcccgcctt
     1141 tgagccggca cactccatgg atccgggatc atccggaatg aaaaaatatg gtgggcagga
     1201 caggattcga acctgtgtag gccgtcggcc
//
LOCUS       DASTJJ010000683         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_462050, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000683 DASTJJ010000000
VERSION     DASTJJ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462050"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFR02_08325"
     CDS             <1..598
                     /locus_tag="VFR02_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870485.1"
                     /translation="LHELRVRRLPMAPAAQALCLAAALGLLLYTVQPILAGTGEKLGR
                     QCRQMSFPEMARRFLRTAHDLPRDGDFAAAAGRLMGKYSGGQKFPAYFFGMEGVEVAM
                     YDGRSKAYPYDDPDQALIVRRTVERILSTDPPLRPGDCVYTSSGMHYGKPVLGGKRRL
                     EEALFDRLRSRYALQRVESSGGVTVYRVMGAKGDGGDP"
     gene            complement(660..1061)
                     /locus_tag="VFR02_08330"
                     /pseudo
     CDS             complement(660..1061)
                     /locus_tag="VFR02_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
BASE COUNT          164 a          354 c          350 g          193 t
ORIGIN      
        1 gctccatgag ctgcgggtcc gtcggctgcc catggccccg gccgcccagg ccctttgcct
       61 ggccgccgcc ctggggctcc tgctatacac ggtccaaccc atcctcgcgg gcaccgggga
      121 aaaactgggc cggcaatgcc gacagatgag cttcccggaa atggcccgcc ggttcctgcg
      181 gaccgcccac gacctgcccc gcgacgggga tttcgccgcg gccgccgggc gcctgatggg
      241 gaaatattcg gggggccaaa agttcccggc ctacttcttc ggcatggagg gggtggaggt
      301 ggccatgtat gacggccggt ccaaggccta tccctacgac gaccccgacc aggccctcat
      361 cgtccgacgg accgtggaac ggatcctgtc caccgatccc cccctgaggc ccggggattg
      421 cgtctatacc tcatcgggga tgcactatgg gaaacccgtc ctggggggta agcgccggct
      481 ggaagaggcc ctcttcgacc ggttgcggtc ccgctacgcc cttcagcgcg tggagagcag
      541 cggcggcgtc acggtctacc gggtgatggg cgccaagggg gacggcgggg acccctaggg
      601 tcttccgccg ttcctcagca cttccccact tcgaggctgt atttctcggt ctcctcgtcg
      661 aatgccacgg gatacttgcc cgtgaaacag gccacgcaat ggccctgggg ggtcccctcc
      721 acggccttca tcatgccctc caccgacagg tagttgaggc tgtccacccg caggtaggtc
      781 ttgatctcgt ccagggtgtg ggtggccgcg atcagttcct tgcggctggg gaaatccatg
      841 ccgtagaagc agggcgagat gatgggcggc gaggtgatgc gcaggtgcac ttccttggcc
      901 ccgacgttgc ggatcatctt cacgatcttt cgcatggtgg tgccgcgcac gatggaatcg
      961 tccaccagga tgacgcgctt cccctccagg gcctcccgca cggcgttgta cttcaaccgg
     1021 gcgccgaagt cgcggatggc ctggtcgggc tcgatgaagg t
//
LOCUS       DASTJJ010000684         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1317773, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000684 DASTJJ010000000
VERSION     DASTJJ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317773"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFR02_08335"
     CDS             complement(<1..578)
                     /locus_tag="VFR02_08335"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HET9870486.1"
                     /translation="MNRRLIPLILLAGWAASPAAWAQGVGRQQLTGYRTFPASQALSA
                     AGLSAPETLDLSFSLPLRHPDEIEPLLERLYDPADPLYRHFLTMAQFTGRFGPSPADY
                     QSVVAFAQAHHLELTGTYPNRLLVDARGSLADVEAAFHVKLLRFRRPDGSLFHAPDRQ
                     PSVDLDTPLGYVGGLENFQLAHPAGLRHLRPA"
     gene            complement(645..>1069)
                     /locus_tag="VFR02_08340"
     CDS             complement(645..>1069)
                     /locus_tag="VFR02_08340"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HET9870487.1"
                     /translation="QTLIPLETRRAYRRALYRVKAKPLGFILRVGRISRPLAKIFRDE
                     GFSKAAFLTAYNPLGKKQAKGRNQRAHQKLVRELRRRGFAFIPGVGEDPDKKWAGETS
                     LLVLGLSLAQARALGRRFRQNALVFAGPKALPRLVLLR"
BASE COUNT          191 a          335 c          376 g          167 t
ORIGIN      
        1 tgggcgggcc gaaggtgtcg caggcccgcc ggatgggcca attggaagtt ctccagcccc
       61 cccacgtaac ccagcggcgt atccaggtcc accgaaggct ggcggtcggg cgcgtggaag
      121 aggcttccgt ccggacggcg aaaacgcagc aacttgacgt ggaaggccgc ctccacgtcg
      181 gcgaggcttc cccgcgcgtc caccagcagc cggttgggat aggtgccggt cagttccaaa
      241 tgatgggcct gggcgaaggc caccaccgac tggtaatcgg ccggcgaggg gccgaagcgg
      301 cccgtgaatt gggccatcgt gaggaaatgc cggtagaggg ggtcggccgg gtcgtagagc
      361 cgctcgagca gcggttcgat ctcatccgga tggcgcaggg gcaggctgaa ggaaaggtcc
      421 agcgtctcag gggccgacag cccggcggcc gaaagggcct gggaggccgg gaaggtccgg
      481 tagcccgtca gttgctggcg tccaacgcct tgggcccacg cggcggggga agccgcccaa
      541 ccggcgagta ggatgagggg gatgaggcgg cggttcatgg ggccctttgg ttggaatggc
      601 cgaccccgcc attttaccaa tccccccgtc ccgccggtca gcttttagcg caaaagcacc
      661 aaccggggca gcgccttcgg ccccgcgaag accagggcgt tctggcggaa ccggcgtccc
      721 aaggcgcgtg cctgggccaa cgataaaccc aagaccaaaa ggctggtttc ccccgcccat
      781 ttcttgtccg ggtcctcccc caccccgggg atgaaggcaa acccccggcg ccgcagttcc
      841 ctgaccagct tttggtgggc cctttggttg cggcccttgg cctgtttttt tcccaacgga
      901 ttataggcgg tcaggaaagc ggccttggaa aaaccctcgt cccggaaaat tttcgccagg
      961 ggccgggaga tccggcccac gcgcaggatg aagcccaagg gtttggcctt cacccggtaa
     1021 agggcccggc gataggcccg gcgggtctca agcggaatga gggtttgct
//
LOCUS       DASTJJ010000685         1474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_705507, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000685 DASTJJ010000000
VERSION     DASTJJ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705507"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFR02_08345"
     CDS             <1..678
                     /locus_tag="VFR02_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HET9870488.1"
                     /translation="GGHQYVTGGLEKDVHLTGVEIGRDFAEPEWGDLRNVATGDGCPK
                     CGKPLKTGRGIEVGQTFKLGTKYSQAMKCVYLGDDGKDHEAVMGCYGIGVTRTVGAAI
                     EQHNDENGIVWPISLSPYQVHLLCLNPSEPEVAKAADQCYEDLKKAGFEVLYDDRPDA
                     TAGFKFKDADLLGLTLSVRIGARGLKEGLVELKRRREKDVVKVPLAEVLPKVKELAAE
                     EWKLCTP"
     gene            797..>1474
                     /locus_tag="VFR02_08350"
     CDS             797..>1474
                     /locus_tag="VFR02_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9870489.1"
                     /translation="MKIALVTDTYYPRINGVSASTQIFAEEFDKLGHEVHIYAPWFPA
                     HLDEAGHIQVHRFPSLYLFFDPEDRLGLPFLDRKLVKHLIDQKYDIVHTQTPFTIGGP
                     AVKWARKSGAKVVHTYHTLFAAYTEHYLRFMPKSWGIAYAKSASRNYCNSCDLVITPS
                     TEMRNTLLGYHVTKPIEVIPTGIRLGRFQGRDPQRYRELKGYGPSDKLLLFMGRVAEE
                     KNIDFLLR"
BASE COUNT          299 a          476 c          450 g          249 t
ORIGIN      
        1 ggcggccacc aatacgtgac cggcggcctg gagaaggacg tgcacctcac gggcgtggag
       61 atcggccggg atttcgccga gcccgaatgg ggcgatctcc ggaacgtcgc gaccggcgac
      121 ggctgtccca agtgcggcaa gcccctcaag accggccggg gcatcgaggt gggacagacc
      181 ttcaagttgg gcaccaaata ttcccaggcc atgaagtgcg tgtacctcgg cgacgacggc
      241 aaggatcatg aggcggtcat gggctgctac ggcatcgggg tcacccgcac ggtgggcgcg
      301 gccatcgagc agcacaacga cgagaacggg atcgtctggc ccatctcgct gtcgccctac
      361 caggtgcacc tcttgtgcct caacccctcc gagcccgagg tggcgaaggc ggccgaccaa
      421 tgttacgagg atttaaagaa ggcgggcttc gaggtcctct atgatgaccg cccggacgcc
      481 acggcgggct tcaagttcaa ggacgcggac ctgctgggcc tgacgctctc ggtgcgcatc
      541 ggcgcccggg gcctgaagga agggttggtg gagctcaagc ggcgccggga aaaggacgtg
      601 gtgaaggtgc ccctggccga ggtgctcccc aaggtgaagg aactggccgc cgaagaatgg
      661 aaactctgca cgccgtaaaa aggatttacc acgaagggca cgaagttcac aaagttcagg
      721 caaccatgaa ttcgattctt ctttaacttt gtgcccttcg tgcactttgt ggtgaaaatg
      781 gtttgaaagg tccgctttga aaatcgccct ggtcaccgac acctactacc cccgcatcaa
      841 cggcgtctcg gcctccaccc agatcttcgc cgaggaattc gacaagctgg gccatgaggt
      901 gcacatctac gccccctggt tcccggccca cctggacgag gcggggcaca tccaggtgca
      961 ccggttcccg tccctctacc tcttcttcga ccccgaggac cggctgggcc tgcccttcct
     1021 ggaccggaag ttggtgaagc acctgatcga ccagaaatac gacatcgtgc acacccaaac
     1081 gcccttcacc atcggggggc cggcggtgaa gtgggcccgc aagtccgggg ccaaggtggt
     1141 gcatacctac cacacgctgt tcgcggccta tacggaacat tacctgcgtt tcatgcccaa
     1201 gagctggggc atcgcctacg ccaagtcggc cagccgcaac tactgcaatt cctgcgacct
     1261 ggtcatcacg ccctccaccg agatgcgcaa caccctcctg ggctaccacg tcaccaagcc
     1321 catcgaggtc atccccacgg gcatccggct gggacgcttc caaggccgcg acccccagcg
     1381 ttaccgggag ctcaagggtt acgggccctc ggacaagctg ctgctcttca tgggccgggt
     1441 ggccgaggaa aagaacatcg acttcctcct gcgg
//
LOCUS       DASTJJ010000686         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_583142, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000686 DASTJJ010000000
VERSION     DASTJJ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583142"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFR02_08355"
     CDS             complement(<1..589)
                     /locus_tag="VFR02_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870490.1"
                     /translation="MRNVILIVLGLAACVPWPLAAAPIATDFLEGFNARYECLAREGL
                     KGFHCRVACSLWDQYLADRRAKWGPRDRRVLALDKVRFTLDYPVKGRLVAGAEPVLKT
                     GGKALEDEAAGVEAAVKGMLLEHLGPWLSLAVRPAGGEAGDPDRKVVLEPGAGGFLVF
                     QESGQPVTEFFDMGGKIFELARGGTEGVQSFKPDLQ"
     gene            complement(656..1738)
                     /locus_tag="VFR02_08360"
     CDS             complement(656..1738)
                     /locus_tag="VFR02_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870491.1"
                     /translation="MNFKRLLWGALCWGVVAAAGADPLPFNLTLYGLYGAPTQPDALV
                     RNNYDGLGFGGQFEWNPSYYASLGLGYEHSDFYGSTPVSAGTLGLEGKFYLAAFIRDF
                     APYLSAGMGLNLGSAGWTGPAGWQVGLGEKVPFIGPTLLDIGFDYHWMDAPGAFEFGA
                     LHAGVGFAFDIAGGPPAPQAPVPTPQASPAVSLTPSVTATVTPTGSPEASPTVTPTQT
                     ATATPGMESPATATPTPEDLQEPTPEATEAPAVSKMKKYYHLGVHHFLKRQYTSALAD
                     LKICVGIQDPKVPSYYYAEAYATMGVIYHFHRTYAGHLDLARKYYRKALKIDPRTKSA
                     KKYMAELDRAGAKTPPAPMDASPTPQ"
     gene            complement(1755..>2181)
                     /locus_tag="VFR02_08365"
     CDS             complement(1755..>2181)
                     /locus_tag="VFR02_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870492.1"
                     /translation="EAESAAMTPVATPMATPLGTPAPLAQAAASPVPTAAPEDTDAPK
                     EVSGVLKMKDVYELGMKSYKQDDYVQAIRYLKQALTLHDPYTPSFYYAEDNAMLGVIY
                     QFHIVHPDLAYQYYHEALRIDPTTETAKKHIDEVKPGQN"
BASE COUNT          363 a          700 c          744 g          374 t
ORIGIN      
        1 tttggagatc gggtttgaag gactggacgc cctccgttcc gccccgcgcc agctcgaaga
       61 tcttgccccc catgtcgaag aactcggtga ccggctgtcc cgattcctgg aagaccagga
      121 aacccccggc ccccggctcg aggacgacct tccggtccgg atccccggcc tccccgcccg
      181 cgggccgcac cgccagggaa agccagggcc ccaggtgctc caacaacatg cccttcacgg
      241 ccgcctccac cccggcggcc tcatcctcca aggccttgcc gccggtcttg aggacgggct
      301 cggcccccgc caccaaacgg cccttcaccg gatagtccag cgtgaagcgg accttgtcca
      361 aggccaacac ccgccggtcc cgggggcccc acttggcccg gcggtcggcc aggtactggt
      421 cccaaaggct gcaggccacc cggcagtgga accccttcaa accctcccgg gccaggcatt
      481 catagcgggc gttgaacccc tccaggaagt ccgtggcgat gggggccgcg gcgaggggcc
      541 aagggacaca ggccgccagg cccaggacga tcaggatgac gtttcgcatg gcggtccttc
      601 cggaaaaaaa taaaaggccg gcccgagggg ccggcctgga ggattgggtc ggggtttatt
      661 gcggcgtcgg cgaggcgtcc atgggcgccg ggggggtctt ggcgcccgca cggtccaatt
      721 cggccatgta ctttttggcg gacttggtcc gggggtcgat cttcaaggcc ttgcggtaat
      781 acttgcgggc caggtccagg tgtcccgcgt aggtccgatg gaaatggtaa atgaccccca
      841 tggtcgcgta ggcctcggca tagtaatagg acggcacctt ggggtcctgg atgcccacgc
      901 atatcttgag gtccgccagg gcggacgtgt actgacgctt gaggaaatgg tggacgccca
      961 ggtggtagta cttcttcatt ttggagaccg ccggggcttc cgtggcctcg ggcgtgggtt
     1021 cctgcaggtc ctcgggcgtc ggagtggccg tggccgggct ttccatcccg ggcgtggcgg
     1081 tcgccgtctg cgtgggtgtc actgtggggc tggcctcggg cgaacctgtc ggcgtcaccg
     1141 tggcggtcac ggaaggcgtg agggagaccg cggggctcgc ctggggggtg gggaccggcg
     1201 cctggggggc gggggggccg cccgcgatgt cgaaggcgaa cccgacgccg gcgtgcaggg
     1261 cgccgaactc aaaagccccc ggcgcgtcca tccagtggta gtcgaacccg atgtccaaga
     1321 gggtcggccc gatgaaaggg accttttccc ccaggccgac ctgccatccc gcgggccccg
     1381 tccatcccgc ggaccccaga ttgaggccca tgcccgccga aagatagggc gcgaaatccc
     1441 ggatgaaggc cgccagatag aactttccct ccaggcccaa ggtgccggcg gagaccggcg
     1501 tggagccgta gaagtcgctg tgttcatacc ccaggcccaa ggaggcataa taagaggggt
     1561 tccattcgaa ctgcccaccg aaccccaggc catcgtagtt attgcgtacc aaggcgtccg
     1621 gctgggtggg ggccccatag aggccgtaaa gggtcaggtt gaaaggcagg ggatccgccc
     1681 cggccgccgc caccacgccc caacacaaag ccccccacag cagtcgttta aaattcacgc
     1741 ggcctcccac ctggttagtt ctgacccggt ttcacctcgt cgatatgctt tttggccgtc
     1801 tcggtggtcg ggtcgatcct caaggcctca tgataatact gatacgccag gtccgggtga
     1861 acaatgtgga actggtagat gacgcccagc atggcgttgt cctccgcgta gtaaaagctc
     1921 ggggtatagg ggtcgtggag ggtcagggcc tgtttcaagt accggatggc ctggacgtaa
     1981 tcgtcctgct tgtaggattt catgcccagc tcatagacgt ccttcatctt gaggacgccg
     2041 ctgacctcct tgggcgcgtc ggtatcttcc ggcgcggcgg tgggcaccgg cgaagcggct
     2101 gcctgggcca ggggggccgg ggtccccagg ggcgtggcca tgggcgtcgc cacgggggtc
     2161 atggccgcgg attcggcttc c
//
LOCUS       DASTJJ010000687         1993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1073489, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000687 DASTJJ010000000
VERSION     DASTJJ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073489"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..502)
                     /locus_tag="VFR02_08370"
     CDS             complement(59..502)
                     /locus_tag="VFR02_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET9870493.1"
                     /translation="MAEPLNFKQNRDWGVTDYYFIDLVIMLASSAKIDLGEMVHPYYK
                     DKDLNLPRARVTINMIASLIKKTTGNLNIEEKKVLREVLEDLQKTYVDKSKQAQERAN
                     EKAKAAPPRPLLPTPPGTGKTDVKDWIHMVQQEIAKKKQNPPAKP"
     gene            complement(513..1142)
                     /locus_tag="VFR02_08375"
     CDS             complement(513..1142)
                     /locus_tag="VFR02_08375"
                     /inference="COORDINATES: protein motif:HMM:NF039337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HET9870494.1"
                     /translation="MPPFFDPDRFLEILRPDRCPLCYLIHDYGQTYLRNLLEESVTDP
                     HTRDRLLASRGFCRRHAWHAVGQAQALGMAVLYAHLLKDGLKRAGPPKRWWGKKERCE
                     VCEAEAELGSVQAGQFAAAWSHSAQLREAFEARGLFCLPHLEKTLAKRMPAARRAHLK
                     RAAEKALQPLLKDLGEFLEKQDYHRSKEAMGPEWDAWIRAVRAVAGERD"
     gene            complement(1177..1704)
                     /locus_tag="VFR02_08380"
     CDS             complement(1177..1704)
                     /locus_tag="VFR02_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870495.1"
                     /translation="MKSSLWQNLLFVALLGSSTVLLPACGGNAVKSSDESTTMMNPEA
                     TPQPAMASEDQGTRSPIRSYVEKAHHPAAASQPAAMASAPTPMPTLAVAATTPVVETP
                     APAQKGGSHFLWWLLLLLILAGIGWYFWSKNHNDEDEFQPHPPSGGLSPVSGYTGTKE
                     LEEKKTPSIWTRKLF"
BASE COUNT          331 a          559 c          703 g          400 t
ORIGIN      
        1 tattggtaac ggggccgccc ttgggcgtgt gtggcgaaga ttagccttgg atcatgcctt
       61 aaggtttggc gggcgggttc tgctttttct tggcgatctc ttgctggacc atgtggatcc
      121 agtccttcac gtcggtcttt cccgtgcccg ggggcgtggg caacagcggg cggggcgggg
      181 cggccttggc cttttcgttg gccctttcct gggcctgttt ggacttgtcc acgtaggtct
      241 tctgcaggtc ttccagcact tcccgcaaca cctttttttc ctcgatgttc aggttgccgg
      301 tggtcttttt gatgagcgag gcgatcatgt tgatggtcac ccgggcccgg ggcaggttga
      361 ggtccttgtc cttatagtaa ggatgcacca tctcgcccag gtcgatcttg gccgaggagg
      421 ccagcatgat gaccaggtcg atgaagtaat agtccgtgac gccccagtcc cggttctgtt
      481 tgaagttgag cggctcggcc atgatacctt cctcagtccc tttccccggc cacggcccgg
      541 accgcgcgga tccaggcgtc ccattcgggg cccatcgctt ccttcgaccg atgatagtcc
      601 tgtttttcga ggaattcacc cagatctttg agcaggggct gcagggcttt ttcggccgcc
      661 cgtttcaggt gggcgcgccg ggcggcgggc atgcgcttcg cgagggtctt ttccaaatgg
      721 ggcaggcaga aaaggccccg ggcctcgaag gcctcgcgca gctgggcgga gtgggaccag
      781 gcggcggcga actgcccggc ctggacggat cccagctcgg cctccgcctc gcagacctcg
      841 cagcgttcct tcttgcccca ccaccgcttg ggcggccccg cgcgcttgag cccgtccttc
      901 agcaggtggg cgtagagcac ggccatgccc agcgcctggg cctggcccac ggcgtgccag
      961 gcgtgccggc ggcagaagcc gcgcgacgcg agcaaccggt cccgggtgtg gggatcggtc
     1021 acgctctctt ccagtaggtt gcgcaggtag gtctggccgt agtcgtggat gaggtagcag
     1081 aggggacaac ggtccggccg gaggatctcc aaaaaacggt ccgggtcgaa aaaggggggc
     1141 acgcgggagc tcaggcccaa cggccgggcg tcgggcttag aaaagtttcc gggtccaaat
     1201 ggacggggtt ttcttttctt ccagctcctt ggtgccggtg tagccgctca cgggcgacag
     1261 gccgcccgag ggcggatggg gctggaactc gtcctcgtca ttgtggttct tgctccagaa
     1321 ataccagccg atgccggcca ggatgaggag caacagcagc caccacagga aatgcgaacc
     1381 gcccttctgg gccggggccg gcgtttccac cacgggggtg gtggcggcga cggccagggt
     1441 gggcatgggg gtcggggcgg aggccatggc cgcgggttgg gaagcggcgg ccggatggtg
     1501 cgccttctcc acgtagctgc ggatggggga ccgggtgccc tggtcctcgg aggccatggc
     1561 gggttggggc gtggcttcgg ggttcatcat ggtggtggat tcgtcggagc ttttcaccgc
     1621 gttgccgccg caggccggca agagcacggt ggaggatccc aaaagggcga cgaagagcag
     1681 gttttgccac agggaagatt tcacggaacc ctcgcgtttc ggggcttggc cccacaggtt
     1741 acgaccagga gccattttat ccaaatccgg gcctaaaggg ggaaggaaaa ggaaggaccg
     1801 gggcaagggg ggtaagaaaa ataggcaaaa tcgaggataa ccgggccccg tcatcgtttg
     1861 gatggtcaag gatggatacc ctctccccgg gagggagagg gccagggtga gggggatttc
     1921 aaaaacagaa ttaggggttc agatcctatt taacttgggc cattagaaag ggcaccctta
     1981 ggggatgccc ttt
//
LOCUS       DASTJJ010000688         1264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_827720, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000688 DASTJJ010000000
VERSION     DASTJJ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827720"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1102
                     /locus_tag="VFR02_08385"
     CDS             68..1102
                     /locus_tag="VFR02_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9870496.1"
                     /translation="MVAGVSALNPRVVTLGTAGGPMWWTGQGAGARSGIATAVLVKDR
                     CYLVDCGHGVGRQLMLAGIGISSISGIFLTHLHSDHTIDLGSLAIFGLYDLKRGDKIK
                     LMGPGDRGMLPRPSGRPAEAPVPVFPDRPTPGTVALFDAILGGYATDLNDRIFDTLRP
                     SPYDFLDVEDIALPEGIGYHPNDNPAPDMEPFEIFSDELVTVTAILVRHAPVAPAFAF
                     RFDTAEGSVTISGDTGRCGNVVRLARNTDLLLHEAIDLDWVHRDRPGVPAESLQAAID
                     HHTHAHTSVADCVAVATEAGAKALALHHLVPGTAPKAVWWRGAGGFAGEYLVPDDLEE
                     ISFAGTATRG"
BASE COUNT          197 a          410 c          439 g          218 t
ORIGIN      
        1 ggtccaacgg cgcggagagc gaccctgcca gcttcgaatc cgatgggctg gtcaaggcgt
       61 gacttcaatg gtggccggtg tgtcggcctt gaaccccagg gtcgtcacgc tgggcactgc
      121 cggcggcccc atgtggtgga ccgggcaggg ggccggagca cgcagcggca tagccacggc
      181 agtattggtc aaggaccgct gctaccttgt tgactgcggg catggggtcg ggcggcaatt
      241 gatgctcgcc ggcatcggca tctcctcgat ctccgggatc ttcctgacac acctccattc
      301 ggaccacacg atcgaccttg ggtccctggc gattttcggc ctctacgacc tgaaacgggg
      361 agacaagatc aagctcatgg gcccggggga ccgcggcatg ttgccccggc catccgggcg
      421 gcccgcggag gcgcccgtgc cggtgtttcc ggaccggccc accccgggga cggtggcctt
      481 gtttgatgcc atcctgggag gttatgccac cgacctcaat gaccggattt tcgacaccct
      541 gcggccctcg ccctacgatt tccttgacgt ggaggacatt gccctcccgg aagggattgg
      601 ctaccacccc aatgacaacc cggccccgga catggaaccg tttgagattt tctccgacga
      661 actggtgacc gtcacggcga ttctggtccg gcacgcgccg gtggcgcccg cctttgcctt
      721 ccgcttcgac actgccgagg ggtctgtgac catctcgggg gacaccgggc gctgcggcaa
      781 cgtggtgcgg ctggcgcgca ataccgacct gctgctccat gaggccattg acctggactg
      841 ggtgcatcgc gaccggcccg gggttccggc ggaatcgttg caggccgcga tcgaccacca
      901 cacccacgcc cacacatccg tcgcggactg tgttgcggtg gccacggaag cgggcgcgaa
      961 ggcgctggca ctgcatcatt tggtgccagg cacggctccc aaagcggtgt ggtggcgcgg
     1021 ggcgggcgga tttgccggcg agtacctggt ccccgacgac ctcgaggaga tttccttcgc
     1081 aggcacggct acgcgggggt agctgcggcg aagccgacgg gcggcagcgc cggcaaaacc
     1141 tcccgtgggg cgggcagacg ctgtacgact ggttcggcgc gcagcgataa attggggcgg
     1201 gcctgcgggt tcaggcgccc cgacttgccc ggcggatcct tccaggaaat gacggggccg
     1261 ggtg
//
LOCUS       DASTJJ010000689         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_219165, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000689 DASTJJ010000000
VERSION     DASTJJ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219165"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFR02_08390"
     CDS             <1..382
                     /locus_tag="VFR02_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9870497.1"
                     /translation="DISAVTTASMAIGIGADYEIYLLFRFREELARSGTVLKATRESL
                     LTSGKAVIFVALSVIGGYGVLQASGFAFYVNLSTLVTTTMTISALFALFFLRALMMLF
                     KPKFIFGEKPEEYFTPKNLKGEMA"
     gene            379..1185
                     /locus_tag="VFR02_08395"
     CDS             379..1185
                     /locus_tag="VFR02_08395"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET9870498.1"
                     /translation="MRTTPKILWLGILAVLGTASPGLPQAALTADQVMEKNFYAAKVK
                     TLKADSDLVLINAQGDKRVRKVSNLQALQPNGVDSKLLVKFLDPADIRGTGFLQIEHS
                     DADDDQWVYLPALQKSRRLVANNKKDSFFGSDFSYGDITLPKPKAYVNTLKGSQAVDG
                     IDCYVVESVPASESLKEDTGYGKKVSWIRKDDFIEAKVEYYDLSGQLLKVQVTGGVTK
                     AGSNPDRYLVAHREMDNVQTGHKTTLDFTHVQTGVAVDDGVFSTRSLERQ"
     gene            1182..>2306
                     /locus_tag="VFR02_08400"
     CDS             1182..>2306
                     /locus_tag="VFR02_08400"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HET9870499.1"
                     /translation="MSRHRAVGRPGTAGRRLRAVLLGVLALAPAGAFAEGDWLSQFWQ
                     DERVSVFLRTDYFGNSRLLDDRKDLWGDTFQLQARPSLGDVDGKLNLRVTDPDPFRGD
                     AAAFDLVEGYVAFHLGPLDARVGKISVPWGRADGVNPTDNLTPHDYTLQLPFDEDLRE
                     GLAALKLDYYLDEEMDLTVFTTPFFEPTKFPIPPSGNGTSYRVTLPGQTLRNEELGLK
                     LNKTGGDWDWSASYFHGFRTLPGLWLVSESAGATVVGVGYDRVDVWGADFAHNLGPFG
                     VRGEAAYGSPEKDPEGPPALVGPYFMAVLGVDRSFDQELNVNLQVIGRFQAAPLAAPG
                     TGAAGQVARLNAIVNNQQDPQGYELSFRVGDKWMNETLGAE"
BASE COUNT          397 a          762 c          780 g          367 t
ORIGIN      
        1 cgacatcagc gcggtgacca cggcctccat ggccatcggc atcggggcgg actacgagat
       61 ctacctgctc ttccgcttta gggaggagct ggcccggtcg ggcaccgtgc tcaaggccac
      121 gcgggagtcg ctgctcacct cgggcaaggc ggtcatcttc gtggccctgt cggtcatcgg
      181 cggctacggg gtgctgcagg cctcgggttt cgccttctac gtgaacctgt ccaccctggt
      241 gaccaccacc atgaccatca gcgcgctctt cgccctcttc ttcctgcgcg ccctgatgat
      301 gctcttcaag cccaagttca tcttcggcga gaagccggag gaatatttca cccccaagaa
      361 cctcaaagga gaaatggcat gagaacgacc ccgaagatcc tgtggctggg catcctggcg
      421 gtcctgggga cggcctcccc gggcctcccc caggcggccc tgaccgccga ccaggtcatg
      481 gaaaagaact tctacgccgc caaggtcaag acgctcaagg ccgacagcga cctggtgctc
      541 atcaacgccc agggcgacaa gcgggtgcgc aaggtgtcca acctgcaggc gctccaaccc
      601 aacggggtgg actccaagtt gctggtcaag ttcctggacc cggcggacat ccgggggacg
      661 ggcttcctgc agatcgagca ttcggacgcg gacgacgacc agtgggtcta cctgccggcc
      721 ctgcagaaga gccggcggct ggtggccaac aacaagaagg acagcttctt cgggtcggac
      781 ttctcctacg gcgacatcac cctgcccaag cccaaggcct acgtgaacac cctgaagggt
      841 tcccaggccg tggacggcat cgactgctat gtggtcgagt ccgtccccgc cagcgagtcg
      901 ctgaaggagg acacgggcta cggcaagaag gtctcctgga tccgcaagga cgacttcatc
      961 gaggccaagg tggaatatta cgacctgtcg ggccaactgc tgaaggtgca ggtcaccggc
     1021 ggcgtcacga aggcggggtc caacccggac cgctacctgg tggcgcaccg ggagatggac
     1081 aacgtccaga ccggccacaa gaccacgctc gacttcaccc acgtccagac cggggtggcg
     1141 gtggatgacg gggtcttttc cacccgctcc ctggagcgtc agtgagccgt catagggcgg
     1201 tgggccgtcc tgggacggcg ggccgccgcc tccgggcggt cctgctcggc gtcctggccc
     1261 tggccccggc gggggccttc gccgaggggg actggctttc ccagttctgg caggatgagc
     1321 gcgtcagcgt cttcctcagg accgattatt tcgggaactc gcgcctcctg gacgaccgca
     1381 aagacctctg gggcgacacc ttccagctcc aggcccggcc ctcgctgggc gacgtggacg
     1441 gcaagttgaa cctgcgggtg acggacccgg accccttcag gggcgacgcc gcggccttcg
     1501 acctggtgga gggctatgtg gccttccacc tgggcccctt ggacgcgcgg gtggggaaga
     1561 tcagcgtgcc ctggggccgg gccgacggcg tcaacccgac ggacaacctc acgccccacg
     1621 actacaccct gcaactgccc ttcgacgagg acctgcggga gggcctggcg gccctgaagc
     1681 tggactatta cctggacgag gagatggacc tgacggtctt caccacgccc tttttcgagc
     1741 cgaccaagtt cccaatcccg ccctcgggga acgggacctc ctaccgcgtc accctgcccg
     1801 gccagaccct ccgcaacgag gagctggggt tgaagctcaa caagaccggc ggggactggg
     1861 actggtcggc cagctatttc cacggcttcc gcacgctgcc gggcctttgg ctggtctccg
     1921 agagcgccgg cgccacggtg gtgggggtgg gctatgaccg cgtggatgtt tggggcgcgg
     1981 acttcgccca caacctgggc cccttcgggg tgagggggga agcggcctat gggtcgcccg
     2041 agaaggaccc ggaagggccg ccggcgctgg tggggcccta tttcatggcg gtcctgggcg
     2101 tggaccgcag cttcgaccag gaactgaacg tgaacctgca ggtgatcggc cggttccagg
     2161 ccgcgccgct cgcggcgccg gggacggggg ccgcggggca ggtggcccgg ttgaacgcca
     2221 tcgtgaacaa ccagcaggac ccccagggct acgagctgag cttccgggtg ggggacaagt
     2281 ggatgaacga gaccttgggc gcggag
//
LOCUS       DASTJJ010000690         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_583547, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000690 DASTJJ010000000
VERSION     DASTJJ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583547"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFR02_08405"
     CDS             complement(<1..1065)
                     /locus_tag="VFR02_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870500.1"
                     /translation="MRSGPLSHPARRMAGAALLAALAWPPSGLQAQMTDRYITQASFQ
                     VSADQRADLWLNGHWLFTQDYTPLQGGPQTHLARPDHLCYFQRDNTLAIRVKSPGHPG
                     IGVAYLLRLVLSDGSHQIFTSSETDQHLALYLTDGLEPYGWQQPGFDDHAWGKAYDPG
                     HVPYAALLPDPDGEVVGFLSADGDNRVHQPGEEHLFRIRFHLDVLPNPRCAPAEPRRS
                     PTPIPFPAEDLPSPVPTPTWKAPALPTVPERRPRSVQVVDSWEAPETLEVHLAPTPPP
                     SPTPPPPPSPTPTPGDLQPALSFYVSLMDGPGVDQLDVVDAAGTPLRTLLKEDLTGSG
                     QVWVRWDGRDARGVALPRGNY"
BASE COUNT          189 a          383 c          448 g          172 t
ORIGIN      
        1 gtaattcccc cggggcaagg cgacaccccg cgcgtcccgc ccgtcccacc gcacccagac
       61 ctggccgctg ccggtcaggt cctcctttaa aagggtccgc aagggggttc ccgcggcgtc
      121 caccacgtcc aattgatcga cccccggacc gtccatcagg ctcacgtaaa agctcaaggc
      181 gggttgcagg tcccccggcg tcggggtggg gctgggcggt gggggcggcg tgggcgacgg
      241 cggcggggtc ggcgccaagt ggacctccag ggtctccggc gcttcccacg aatccaccac
      301 ttggacggac cgggggcggc gctccggaac ggtggggagc gcgggggcct tccaggtcgg
      361 cgtagggacc ggggacggca ggtcctcagc cgggaaaggg atgggcgtgg ggctgcgccg
      421 gggttcggcc ggcgcgcagc ggggattggg cagcacatcc aggtggaacc ggatgcggaa
      481 gaggtgctcc tcccccggct ggtggacccg gttgtcgccg tcggcggaca aaaagcccac
      541 cacctccccg tccggatcgg gcaggagggc cgcgtagggc acgtgccccg gatcgtaggc
      601 cttgccccag gcgtggtcgt cgaaccccgg ctgttgccac ccatagggtt ccaggccgtc
      661 cgtcaggtag agcgccaagt gttggtccgt ctcgctgctg gtgaatatct ggtggctgcc
      721 gtcggacagg accaaccgca gcaggtaggc cactccgatg cccgggtggc cggggctttt
      781 cacccggatg gccagggtgt tgtcgcgttg gaaataacag aggtggtccg gccgggccag
      841 gtgggtctgg gggccgcctt gaagcggcgt atagtcctgg gtgaagagcc aatgcccgtt
      901 cagccaaaga tcggcccgtt ggtccgccga gacctggaaa gaggcctggg tgatgtagcg
      961 gtcggtcatc tgggcctgga gcccggaggg cggccaggcc agcgccgcca gcagggcggc
     1021 cccggccatc cggcgcgcgg gatgggaaag gggtccggaa cgcatggtcc gtagtataaa
     1081 ctgaaaccac gcccggccgc cccaaaaccc gccgggcccg gggaccatgg acgagaaaac
     1141 caccttacgc tgtaaagtcg gacccgcggc cgatggccgg cccttgctgg at
//
LOCUS       DASTJJ010000691         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_219294, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000691 DASTJJ010000000
VERSION     DASTJJ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219294"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFR02_08410"
     CDS             complement(<1..193)
                     /locus_tag="VFR02_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9870501.1"
                     /translation="MAAIDLDSVKNLSLFQDLSTAELENLSLLIFEKNYSKGSTLFVE
                     GMTGEVLYLVKSGSVQILKK"
     gene            complement(254..>1048)
                     /locus_tag="VFR02_08415"
     CDS             complement(254..>1048)
                     /locus_tag="VFR02_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9870502.1"
                     /translation="RFVPAPRVAEVVKGAFTRENSVSGYNATFLYPERGGISALPRAL
                     GEGLPNLWTGVGLTGLDLKRRRARLSNGLEVRYGRLVSSLPLPALLRLSEGLPPAVRR
                     AAASLKATSIYNVNFGFRGPQPVSYSWVYFPGKEFLFHRAGSVSACVPGQAPPGHFSL
                     YVEFSYGPGRRPDPAAWGRHALARLRELGWVKPGQAPVTRVDLDLPGAYVVYDAAREE
                     RVGALTGFLARRGVQTVGRYGLWEYGSMESAMDQGRRAARALLAGA"
BASE COUNT          180 a          359 c          362 g          147 t
ORIGIN      
        1 gtttcttcag gatctggacc gacccggact tcaccaggta aagcacctcg ccggtcatgc
       61 cttccacgaa cagcgtcgag cccttcgagt agttcttctc gaagatgagg agcgacaggt
      121 tctccaattc ggccgtggag aggtcttgga agaggctgag gttcttgacg gaatccagat
      181 cgatggcggc catggtcccc ccggggcccg gaatggcccg tattatacac gggatcgggg
      241 ccggcgggct tctttaggcc cccgccaaaa gggcccgggc ggcccggcgg ccttggtcca
      301 tggcgctttc catgctgccg tattcccaca ggccgtaacg tcccacggtt tggacgcccc
      361 ggcgggccaa aaagcccgtc aaggcgccca cccgttcctc ccgggccgcg tcatagacca
      421 cgtaggcgcc cggcaggtcg aggtccaccc gcgtcacggg ggcctggccc ggcttcaccc
      481 agcccagttc ccgaagccgg gcgagggcgt gccgtcccca cgcggccgga tcggggcggc
      541 ggcccgggcc gtaggaaaat tccacgtaga gggaaaaatg ccccggcggg gcctggcccg
      601 gcacgcaggc gctcaccgaa ccggcccggt ggaagaggaa ctccttgccc gggaaataga
      661 cccagctgta ggacacgggc tgggggcccc ggaagccgaa attgacgttg tagatggacg
      721 tggctttcag ggaggcggcg gcccgccgga ccgccggggg caggccttcc gaaaggcgca
      781 agagcgccgg caggggcagg ctggagacca gccgtccata gcgcacctcc aggccgttgg
      841 aaaggcgggc ccggcgccgc ttcaggtcca gccccgtcag tcccacgccc gtccaaaggt
      901 tgggcaaccc ctcgcccagg gcccggggca gggccgagat gccgccccgc tcggggtaca
      961 ggaaggtggc gttgtagccc gagaccgaat tttcccgcgt gaaggccccc ttcaccacct
     1021 ccgccacccg gggggcgggc acgaagcg
//
LOCUS       DASTJJ010000692         1227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1318685, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000692 DASTJJ010000000
VERSION     DASTJJ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318685"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VFR02_08420"
     CDS             <1..1014
                     /locus_tag="VFR02_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9870503.1"
                     /translation="ATPVFISNTRLTTKPNAFYVNPGSTSVFVAEGGVSLGGFVSQFE
                     TYNVGDVYASTNGNALEIGVPTPGEGPVSYFERLPGTGVVNPQGLAYSLGWWATLDSP
                     GVIGATGSAVMYEGDDFFGSGFPAFLMPVESTTTSYGSGAFKNPRSIAGDSAGNFYVA
                     DTGNHRVLIFSTGGYNATSIGTAIPRGAWTGSNTYNFQNPVAVACDSSNNVYVGDVAD
                     MVTGNSVVQEYASQATTIKGSWTLVPGCIINGLAIDGNGDFFVSDVGNGVAGAGQVEE
                     YKILDSSHTLLLRVWGDPKSSNEFSTFYPSCIGLVGNASLTSITNILVGDQNNDMIQV
                     FGP"
BASE COUNT          237 a          356 c          366 g          268 t
ORIGIN      
        1 gccacgccgg tcttcatttc caatacccgg ttgacgacca agcccaacgc cttctatgtc
       61 aacccgggga gcacctccgt ttttgtggcg gaagggggag tgagcctggg tgggtttgtc
      121 tcccaattcg aaacttataa cgtcggggat gtctacgcgt ccacgaacgg gaacgctttg
      181 gagatcgggg ttcccacgcc gggagaaggc cctgtttcct actttgaacg tcttcccggc
      241 acgggggtgg tgaaccccca ggggttggcc tattcgttgg gttggtgggc gaccttggat
      301 tccccgggcg tgatcggggc caccggcagc gcggtgatgt atgaaggcga tgatttcttc
      361 gggagcggct tccccgcctt cctgatgccc gtggaaagca ccacgaccag ctacggctcg
      421 ggcgccttta aaaacccacg ctccatcgcg ggcgattcgg cggggaactt ctacgtcgcc
      481 gataccggga accatcgggt gctcatcttc agcaccgggg gatacaatgc gacgtcgatc
      541 ggcaccgcga tcccgcgggg ggcctggacg ggatccaaca cgtataattt ccagaatccg
      601 gtcgcggtgg cttgcgactc ctccaacaac gtctatgtgg gggacgtggc cgatatggtc
      661 accggcaatt cggtcgttca ggaatacgcc tcacaggcca ccaccatcaa gggcagttgg
      721 accttggtgc ccggttgcat catcaacggc ttggcgatcg acgggaacgg ggacttcttt
      781 gtctcggacg tcggcaatgg ggtggccggt gccggacaag tggaagaata taaaatactc
      841 gattcgagcc ataccctgct gttgagggtt tggggcgacc ccaagagctc gaatgagttt
      901 tccaccttct atccttcttg tatcggcctg gtcgggaacg cgagtttaac gtcgatcacc
      961 aacatcctcg tgggcgacca gaacaatgat atgatccagg tcttcgggcc ctgaccgaag
     1021 gcggtttttg accccgcttt cccccgggaa ggcggggttt tttatttcgg gtgaaaagcg
     1081 ccgaaggctc cgccgtccca agccgacttc cgcctgcgtt ggatattgaa ccttaggagc
     1141 cgccatgccg cacctccacg acaagatcga cttcacggtc tctatcttca tcgtgaagga
     1201 ccaaaaggtc cttttcatcc tgcacaa
//
LOCUS       DASTJJ010000693         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_953134, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000693 DASTJJ010000000
VERSION     DASTJJ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953134"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VFR02_08425"
     CDS             <1..1188
                     /locus_tag="VFR02_08425"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9870504.1"
                     /translation="KDLMTASLNALVDKAKLQGKLLGDVALGAVMNSSSDWNLARECV
                     LSTPLHPETPAYNVQRACGTGLENIWQIALKAHTGAIELGIAGGVDTNSDLPIEVSDK
                     LQKVLLRLNAARTFSQKLKAMEGFSLKILRPKTPTINEPRTHLSMGRHTELMVQEWGI
                     SREEQDAFALKSHQNGAKAYAEGFYDDLVVPFQGLAKDGILRGDTSLERLAKLKPAFD
                     PQKGSLTAGNSSPLTDGSSAVLVGNPRGAERAGLKPLARIVDVQVAAVDFAHGAGLLM
                     APTKAVAALLTRNRLTPADFDYFEIHEAFAGQVLCNLKALESEKYCKEVLGLPGALPP
                     LDRSKLNVVGSSIALGHPFAATGARIAGTLAKLLASGGNKRGLISICTAGGMGIAAVL
                     ETA"
BASE COUNT          265 a          422 c          418 g          235 t
ORIGIN      
        1 aaggacctga tgaccgcttc cctgaacgcc ttggtggaca aggccaagct gcaggggaag
       61 ctcctgggcg acgtggccct gggggcggtg atgaacagtt cctcggattg gaacctggcc
      121 cgggaatgcg tgctttccac cccgctccat cccgagaccc cggcctacaa tgtgcagcgc
      181 gcctgcggca cggggctgga gaacatttgg cagatcgccc tcaaggccca cacgggggcc
      241 atcgaactgg gcatcgcggg gggcgtggac accaacagcg acctgcccat cgaggtctcc
      301 gacaagctcc aaaaggtcct gctgcgcttg aacgcggccc ggaccttttc ccagaaattg
      361 aaggccatgg agggtttttc gctgaagatc ctcaggccca agaccccgac catcaatgaa
      421 ccccgcaccc acctttccat gggccggcat accgagctca tggtccagga gtggggcatt
      481 tcgcgggaag agcaggacgc cttcgcgctc aagagccacc agaacggggc caaggcctat
      541 gccgaagggt tctatgacga cctggtggtc cctttccagg gtttggccaa ggacggcatc
      601 ctgcgggggg acacctcact ggagcggttg gccaagctca agcccgcctt cgacccccaa
      661 aagggcagct tgaccgcggg gaacagctcg cccctgaccg acggctcgtc ggccgtcctg
      721 gtgggcaatc cccggggcgc ggagcgcgcg ggcttgaagc cgctggccag gatcgtggac
      781 gtgcaggtgg cggcggtgga tttcgcccat ggggcgggcc tgctcatggc gcccaccaag
      841 gcggtggccg ccctcctcac ccgcaaccgg ctcaccccgg cggatttcga ctactttgag
      901 atccatgagg ccttcgcggg ccaggtgctc tgtaatttga aggccttgga aagcgaaaag
      961 tactgcaagg aagtcctggg cctgcccggg gccctgccgc ccttggaccg ctccaagctc
     1021 aacgtggtgg gcagcagcat cgccctgggg catcccttcg ccgccaccgg cgcccgcatc
     1081 gccgggaccc tggccaaact gctcgcctcc ggcgggaaca agcgcggcct catctccatc
     1141 tgcacggcgg gcggcatggg catcgcggcg gtgctggaaa cagcttaaga ggctctaaca
     1201 aaacccaaaa tcaattaaca atgatggacg ctgataaacg ccgatgaagc aaaaaataag
     1261 gaaccttggc attttttatc agccttcatc tgcctttatc agtgttaaat aggttttgtt
     1321 aggggttcta agaggttttg
//
LOCUS       DASTJJ010000694         1362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_706498, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000694 DASTJJ010000000
VERSION     DASTJJ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706498"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1362)
                     /locus_tag="VFR02_08430"
     CDS             complement(<1..>1362)
                     /locus_tag="VFR02_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870505.1"
                     /translation="LSLESWAQYSPQGDALYYLREEDGQDAVLRQPLDGLGMPQGEPV
                     TLGGDLGSIRCLQVTPQGLYLTSNRAKKIYNVYRMGLDGKDLTQMTDTYADVLDLSVS
                     PGGSALYACLYQKGQVSLYAFREDALSPRPAGAPDNAPYLADDFSQAARIIPASAPPP
                     VPTPVWRDAGAPPAAAPPAPGSLEVVQATNLVQLKWAPDLRDDQPVEDYRVYRATGPG
                     ALFHAVGKTDNGSLGFFNDFEVEPGVHYFYYVTAENAAGESGPSAVVDAIPESRVSSH
                     DYGIKLTPDILLFLAGYDSSFGFVGGGVVQLSDYLGDHRLSIVGNSIPTVQTGFQLGY
                     EFSQWRTTVDLDLFYYQNFLQIYDLQTGNIVNQYRDNENGFALNFTYPLDTYTRVEYG
                     LGTQRFQGNPLYLQFSEGISNYFQDADRWNVANFYRLAYIKDHRRGTQFWPQSGYSYN
                     LTL"
BASE COUNT          228 a          386 c          503 g          245 t
ORIGIN      
        1 acagggtcag gttgtaggaa taaccgcttt ggggccagaa ctgggtcccc cgccggtggt
       61 ccttgatgta ggccagccgg tagaaattgg ccacgttcca ccggtcggcg tcctggaaat
      121 agttggagat cccctcggag aactgcaggt acagggggtt gccttggaag cgctgggtgc
      181 ccagtccgta ttccacccgg gtgtaggtgt ccaggggata ggtgaagttc agggcgaaac
      241 cgttctcgtt gtcccggtac tggttgacga tgttgccggt ctgcaggtcg tagatctgga
      301 ggaaattctg gtagtaaaag aggtccaggt ccaccgtggt gcgccattgg gagaactcgt
      361 agcccaattg aaagccggtc tgcaccgtgg ggatggagtt gcccacgatg gaaaggcggt
      421 ggtcgcccag gtagtcgctc aattggacca ctcccccgcc cacgaacccg aaggagctgt
      481 cgtagccggc caggaagagc aggatgtcgg gcgtgagctt gatcccatag tcgtgggaac
      541 tcacccggct ctcgggaatg gcgtccacca cggccgaggg gccgctttcc ccggcggcgt
      601 tctcggcggt gacgtagtag aaataatgga cgccgggttc cacctcgaaa tcgttgaaga
      661 agcccaggga accgttgtcg gtcttgccga cggcgtggaa cagcgccccg ggtccggtgg
      721 cccggtagac ccggtaatcc tccacgggtt ggtcgtcccg caggtcgggg gcccacttga
      781 gctgcaccag gttggtggct tggaccactt ccagcgagcc gggcgccggg ggggcggccg
      841 cgggcggggc gccggcgtcc cgccagacgg gcgtggggac gggcggcggc gccgaggcgg
      901 ggatgatccg ggcggcctgg ctgaaatcat cggccaggta gggggcgttg tccggcgcgc
      961 ccgcgggccg gggggataag gcgtcctccc ggaaggcgta gagggacacc tgccccttct
     1021 ggtaaaggca ggcgtaaagg gccgatccgc cgggcgatac cgacaggtcc agcacgtccg
     1081 cgtaggtgtc ggtcatctgg gtcaggtctt tcccgtccag gcccatccgg tagacgttgt
     1141 agatcttctt ggcccggttg gaggtcaggt aaaggccttg gggggtgacc tgcaggcacc
     1201 ggatggatcc caggtccccg cccagcgtca cgggttcccc ttggggcatt cccaggccgt
     1261 cgaggggctg gcgcaggacg gcgtcctggc cgtcctcttc gcgcaggtaa tagagggcgt
     1321 cgccttgggg ggaatattgg gcccaggatt ccagcgaaag gt
//
LOCUS       DASTJJ010000695         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_953348, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000695 DASTJJ010000000
VERSION     DASTJJ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953348"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..656)
                     /locus_tag="VFR02_08435"
     CDS             complement(84..656)
                     /locus_tag="VFR02_08435"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HET9870506.1"
                     /translation="MKNQNNIALKEWATVLKALEEGKQTVLLRKGGIAEEGGHFRPEH
                     PEFFLYPTFEHQSPEDIKPDWRPRLAAIEREHKDAKRIHFRLYAVAEAVEKVTDWEAC
                     KRIIPFTVFSEEAVQKRFHYDDWEGLYLFLVRVYALPVPLDLPHKPSYDGCKSWVPLE
                     TSLFTAGSLPVVPDGAWPYTRDKIIKVLKG"
     gene            complement(708..1580)
                     /locus_tag="VFR02_08440"
     CDS             complement(708..1580)
                     /locus_tag="VFR02_08440"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HET9870507.1"
                     /translation="MALLEALGAVLCLGSYMVPVRLSPAKGAEFMPPLALGTLALTLL
                     FFQGDLRALAAQPGALALALLSGLLWAGGQWMANLALEAISLAKGTLLFSLNTLINIL
                     IGMVLFREVSGPTALLGVAAGALLLFGGAWWVSSAEPASRRQEDLRRGIPLALGAGLF
                     WGAYFVPLKLAPMVDPAFAFPPAVYLSGLALGGCLPGLVFALARRRWSGGARGLAAGA
                     LSAGLWLGGMAFFLSAIDRLGLARAVPIVNSNALVYAAWSLLVFKEIPLSQTPKVLGG
                     TLLALAGILFLAHA"
     gene            complement(1601..2968)
                     /locus_tag="VFR02_08445"
     CDS             complement(1601..2968)
                     /locus_tag="VFR02_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9870508.1"
                     /translation="MIHSLLISLRSLARQWGGGWNGYWVDRPLFLIGRHGLKTRRMFA
                     GLPRRQAVTIYAGVFWTVCLSMTAPYLSLYLVQLGLSETEVGLYQFLMKAVGLAGFFL
                     GGYFSDVWGRKRALITFDVFTWCGYCLCLALAHNKWWGVAAIFFIATNALSLTPYQCL
                     LAEGLSSKKRGHVFAVTQLVNLAPFLLFFPLLGGFWVEKAGLGAACHGMFWLFFACVS
                     AGIAVRWRLLPPSEAYEQAPGAWTHLLREGLAQYARTLAKYLDKPGWKLFLASRLIDE
                     WFIATWAVYASLYYAHYLGLRDSYLSILTQSGAYVAFLVLFFLMPNLSGSRMVRLLGY
                     DQLFGAAAMVLLLFIRPGTGGLLVPCLLAASLAAVGGSLYASLSTAVWMSIIDERERA
                     KVVAASTAFVYIGVLLAGSLASFLYGRLSPPALLVAVLAARVAGFLLLRRVSGILLAP
                     AAKNG"
     gene            complement(3096..>3331)
                     /locus_tag="VFR02_08450"
     CDS             complement(3096..>3331)
                     /locus_tag="VFR02_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870509.1"
                     /translation="FFESRVFRPFALPGKYSYGNYLFHGSVLFFAAPLLSGWGVYPAF
                     LAFAAASTALAALSFHFFEVPANRWVRERLGAR"
BASE COUNT          698 a         1095 c         1131 g          407 t
ORIGIN      
        1 tcaaaggaac ggaaaaaggc ctccagctcc ccggcttccg cggggtaaaa gcggcccgcc
       61 accacggggg ccagcctcac gcctcagccc ttcaacactt tgatgatctt gtcgcgggta
      121 tagggccaag cgccgtccgg gacgacgggc agcgaccccg cggtgaacaa cgaggtctcc
      181 aaaggcaccc aggacttgca gccgtcatac gagggcttgt ggggcaggtc gagcggcacg
      241 ggcagggcgt agacccgcac gaggaacagg tacagtccct cccaatcgtc gtagtggaag
      301 cgcttctgca ccgcctcttc gctgaagacc gtgaagggga tgatccgctt gcaggcctcc
      361 cagtcggtga ccttttccac ggcctccgcg accgcgtaga ggcggaaatg gatgcgcttg
      421 gcgtccttgt gttccctctc gatggcggcc aggcgcgggc gccagtcggg cttgatgtcc
      481 tcgggcgact ggtgctcgaa ggtgggatag aggaagaact cggggtgctc ggggcggaaa
      541 tggccgccct cttccgcgat cccgcccttg cgcagcagga ccgtctgctt cccttcctcc
      601 agggccttca ggaccgtggc ccattccttg agtgcgatgt tgttctggtt tttcatagga
      661 aattgattat gtggatttcc aattgaattg gcaagcccaa aggggcgcta cgcgtgggcg
      721 aggaaaagga tgccggcgag cgccagcagg gtcccgccca agaccttcgg cgtttgggac
      781 aaggggatct ccttgaagac caacagcgac caggcggcgt agaccagcgc gttgctgttc
      841 acgatcggca cggcccgggc caaccccaaa cggtcgatgg ccgaaaggaa aaaggccatg
      901 ccgcccagcc acaggcccgc cgacaaggcg ccggccgcca agccgcgcgc gccgccggac
      961 cagcgccgcc gcgccagcgc gaacaccaac cccggcaggc agccgcccaa ggccaaaccg
     1021 ctcagataga ccgccggggg gaaggcgaag gccgggtcca ccatgggcgc cagcttgagc
     1081 ggcacgaagt aggcgcccca aaaaagcccg gcccccaggg ccaaggggat cccccgccgc
     1141 aggtcctctt gtcggcggga agcgggttcg gcggaagaga cccaccaggc ccccccgaaa
     1201 agcagcagcg cccccgccgc cacgcccagc agcgccgtcg ggcccgagac ttcccggaat
     1261 aggaccatgc cgatgaggat gttgatgagg gtgttcaagc tgaagagcag cgtgcccttg
     1321 gccaggctga tggcctccag ggccaggttg gccatccact ggccgcccgc ccaaagcagg
     1381 cccgataaaa gggccagcgc caaagcgccc ggctgggcgg ccagggcccg caggtccccc
     1441 tggaaaaaga ggagggtgag cgccagggtg cccagggcca ggggcggcat gaactccgcg
     1501 cccttggcgg gcgagaggcg cacgggcacc atataactgc ccaggcaaag caccgccccg
     1561 agcgcttcca acaaggccat aggtcgcttc cctttcgtgg tcagccgttc ttcgccgccg
     1621 gggcgagcag gatgccggag acccggcgca acaagaggaa cccggccacc cgggcggcca
     1681 gcacggccac caacagggcc ggcggcgaca ggcggccgta gaggaacgac gccagcgaac
     1741 ccgccaacag cacgccaata tagacaaagg ccgtggaggc cgccacgact ttcgcccttt
     1801 cccgctcgtc gatgatgctc atccagaccg ccgtgctcag cgaggcgtaa agcgacccgc
     1861 ccaccgcggc cagactggcc gccaagaggc agggcaccag caggccgccg gtcccgggcc
     1921 ggatgaaaag caacaggacc atggcggccg cgccgaaaag ctggtcgtat cccagcaggc
     1981 gcaccatgcg gctcccggac aggttgggca tgaggaagaa gagcaccagg aaggccacgt
     2041 aagcccccga ctgggtcagg atggacagat aggaatcccg gagccccagg taatgggcgt
     2101 aatagaggga ggcatagacc gcccaggtgg cgatgaacca ctcgtcgatg agccgcgagg
     2161 ccaggaacaa tttccaaccg ggcttgtcca ggtacttggc caacgtgcgg gcgtactggg
     2221 ccaggccctc gcgcagcagg tgggtccagg cgcccggcgc ctgctcatag gcctccgagg
     2281 ggggcagcag gcgccaccgc acggcgatcc ccgcgctcac acaggcgaag aagagccaga
     2341 acatgccgtg gcaggccgcc cccaggcccg ccttctccac ccagaacccg cccagcagcg
     2401 ggaagaagag caggaaaggc gccaggttga ccaactgggt cacggcgaag acgtgcccgc
     2461 gtttcttgga cgagagcccc tcggccagca ggcattggta gggggtgagc gacagggcgt
     2521 tggtggcgat gaagaagatg gcggccacgc cccaccattt gttgtgggcc aaggccaggc
     2581 acaggcagta accgcaccag gtgaacacgt cgaaggtgat gagcgcgcgc ttgcggcccc
     2641 acacgtcgga aaagtagccg cccaggaaga accccgccag gcccacggcc ttcatgagga
     2701 actggtagag gcccacctcg gtctcggaca ggcccaattg caccaggtag aggctcaggt
     2761 agggcgcggt catggacagg cacaccgtcc aaaagacccc ggcgtagatg gtgacggcct
     2821 ggcgccgggg gagcccggcg aacatgcgcc gggtcttgag gccgtggcgc ccgatcagga
     2881 acaggggccg gtccacccaa tagccgttcc acccgccgcc ccattggcgg gccagggacc
     2941 tgagggaaat aaggagggaa tggatcacgg ctccgcctga accggttcaa ccgccgggcg
     3001 cgcgcgacaa cggatcggac gcggcggggg gaacggggga tgaaaacggc gccattgtac
     3061 ccgcggcgcg ggggctttcc catccccttt tgcccctacc gggcgccgag ccgctcccgg
     3121 acccagcggt tcgccgggac ctcgaaaaaa tggaaggaca gggccgccag ggcggtggaa
     3181 gccgccgcga aggccaggaa agccggataa acgccccaac ccgacaagag gggcgcggcg
     3241 aagaaaagca ccgagccgtg gaagagatag ttcccataac tatacttccc cggcagggcg
     3301 aagggccgga acacccggga ttcaaagaag g
//
LOCUS       DASTJJ010000696         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_953590, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000696 DASTJJ010000000
VERSION     DASTJJ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953590"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFR02_08455"
     CDS             complement(<1..911)
                     /locus_tag="VFR02_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET9870510.1"
                     /translation="MSQDFIPAAKPIIGAEERAAVDAVMATGMLAQGKQVAEFESEFS
                     HVLLDGRASVAVNSGTSGLHLGLLASGVGPGDEIIVPSFTFAATGNSVALTGATPVFA
                     DIELDHYALDVAHVESLITDKTKGIMPVHLYGHPFAAEAFAALAEKHGIDLYEDAAQA
                     HGAALNGKKVGTFGKFAMFSLYPTKNMTSGEGGMVSTSDATVERNLRLLRNQGMEKQY
                     ENELVGFNNRMTDLHAAIGRVQLTKVEAWTKQRQENAAFLSANLEGVGTPKVAEGAEH
                     VYHQYTIRVPEGRDELHERLRSEYNIGS"
     gene            complement(908..>1191)
                     /locus_tag="VFR02_08460"
     CDS             complement(908..>1191)
                     /locus_tag="VFR02_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET9870511.1"
                     /translation="VGVTIQDGASVGARAVCIAPLTIGRWATIAAGSVVTKDVPAFAL
                     MAGVPAHRLGWVGKAGFPLKEDGGFWVCPETGAKYSEDGTSLKEAEDNA"
BASE COUNT          182 a          384 c          399 g          226 t
ORIGIN      
        1 gagccgatgt tgtactcgga acgcaggcgc tcgtgcagct cgtcccggcc ttccggcacc
       61 cggatggtgt actggtggta cacgtgctcg gcgccctcgg ccaccttcgg ggttcccaca
      121 ccttcgaggt tggcgctgag gaaggctgcg ttctcctggc gctgcttggt ccaggcttcc
      181 accttggtga gctggacgcg gccgatggct gcgtgtaggt ccgtcatgcg gttgttgaag
      241 cccacgagtt cgttttcgta ctgcttctcc atgccctggt tgcgcagaag gcgcaggttc
      301 cgctcaacgg tggcgtccga cgtcgagacc atgccgccct cgcccgaggt catgttcttg
      361 gtcgggtaca ggctgaacat ggcgaacttg ccgaaggtgc ccaccttctt gccgttaagc
      421 gcagcgccgt gggcctgggc cgcgtcctcg tacaggtcga tcccgtgctt ctcggccagg
      481 gcagcaaagg cttcggcggc gaaggggtgc ccgtagaggt gcaccggcat gatgcccttg
      541 gtcttgtccg tgatgaggga ttcgacgtgg gccacgtcca gcgcgtagtg gtccagttca
      601 atgtcggcga agacgggcgt ggcgccggtc agggccacgg agttgccggt ggcggcaaag
      661 gtgaaggacg ggacgatgat ctcgtcgccg gggccgacac cggaggccag caggcccagg
      721 tgcaggcccg aggtcccgga gttcacggca acgctggcac gtccatccag gagcacgtgg
      781 gagaactcgg attcaaactc ggccacctgc ttcccctgcg ccagcatgcc ggtggccatg
      841 acggcgtcga ccgctgcccg ttcctcggct ccgatgatgg gttttgctgc gggaatgaag
      901 tcctggctca tgcgttgtcc tctgcctctt tcagggaagt tccatcttca ctatattttg
      961 ccccggtttc cgggcacacc cagaaaccac cgtcctcctt gagggggaag ccggccttgc
     1021 cgacccaccc caggcggtgg gcgggaacgc ccgccatcaa ggcaaacgcg ggaacgtctt
     1081 tggtgaccac cgagccggcc gcgatggtcg cccaccggcc aatggtcagc ggcgcaatgc
     1141 acacggcccg cgcacccacc gaggcgccgt cctggatggt gacccccacg g
//
LOCUS       DASTJJ010000697         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_953620, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000697 DASTJJ010000000
VERSION     DASTJJ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953620"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1024)
                     /locus_tag="VFR02_08465"
     CDS             complement(<1..>1024)
                     /locus_tag="VFR02_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419145.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET9870512.1"
                     /translation="FGLAKQAREVLNSVFLYHCVQAGMDMAILNPADIKPYAAIPEDE
                     RKLADDLVFNRDPQALAKFIQHFEGKGPVEAQTAVDPTEGMTPAQKAHWKIVNRKPDK
                     IEADLAEVMKSKSAVETLNTVLLPAMKEVGDKFGAGELILPFVLQSAEVMKKAVAYVE
                     QFLDKNASTSKGKVVLATVYGDVHDIGKNLVRTILSNNGYTVYDLGKQVPVSTIIAKA
                     QEVQADAIGLSALLVVTSKQMPICVQELGKLGLQYPVVVGGAAINRRYGYRISFVDEK
                     LYDPGVYYAKDAFEGLDILNALSDLASRETFRKKIKEDALKAKEANFADAVQEEGEAT
                     PDKYPPT"
BASE COUNT          152 a          316 c          338 g          218 t
ORIGIN      
        1 ccgtgggggg atacttatcc ggcgtggctt cgccctcttc ctgcacggcg tccgcgaaat
       61 tggcttcctt ggccttgagg gcgtcctcct tgatcttttt gcggaaggtc tcccgggagg
      121 ccaggtcgga cagggcgttg aggatgtcca acccctcgaa ggcgtccttg gcgtaataga
      181 cgcccgggtc gtagagcttc tcgtccacga agctgatgcg gtagccgtag cggcggttga
      241 tggcggcccc gcccaccacc accggatatt gcaggcccag cttccccagc tcctgcacgc
      301 agatgggcat ctgtttggag gtgaccacca agagcgccga gaggccgatg gcgtcggcct
      361 gcacttcctg ggccttggcg atgatggtgg acaccggcac ctgcttgccc aggtcataga
      421 cggtgtagcc gttgttggac aggatggtgc gcaccaggtt cttgccgatg tcgtgcacgt
      481 cgccgtagac ggtggccaag accaccttgc ctttggaagt ggaggcgttc ttgtcgagga
      541 actgttccac gtaggccacg gccttcttca tgacctcggc ggactgcagc acgaagggca
      601 ggatcagttc cccggcgccg aacttgtccc ccacttcctt catggcggga agcagcacgg
      661 tgttgagggt ctccacggcg ctttttgact tcatcacctc ggccaggtcg gcctcgatct
      721 tgtcgggctt gcggttgacg atcttccaat gcgccttctg ggcgggggtc atgccctccg
      781 tggggtccac ggccgtctgg gcctccacgg gccccttgcc ctcgaaatgc tggatgaact
      841 tggccagcgc ctgggggtcg cggttgaaga ccaggtcgtc ggccagcttg cgctcgtcct
      901 ccgggatggc ggcgtagggc ttgatgtccg ccgggttcag gatggccatg tccatgccgg
      961 cctgcacgca gtggtagagg aagaccgagt tcagcacttc ccgcgcctgc ttggccaggc
     1021 cgaa
//
LOCUS       DASTJJ010000698         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_220170, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000698 DASTJJ010000000
VERSION     DASTJJ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220170"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFR02_08470"
     CDS             complement(<1..315)
                     /locus_tag="VFR02_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870513.1"
                     /translation="MRSHPLPTAAFPELVPLARGVFETAFPWMERWGPLASRGAAVFL
                     ESAGPLEDAGRWCLLAGDPAAELDGRSFPGGPDAVLEFLDAVTQGPPAPFPEGLSRAW
                     FGA"
     gene            complement(405..944)
                     /locus_tag="VFR02_08475"
     CDS             complement(405..944)
                     /locus_tag="VFR02_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870514.1"
                     /translation="MNDILTAQELSAYLKITTTTIYKLAQQGKLPSFKIGSEWRFKKE
                     LIDRWLEKGAQAGQKRVLVVDDEPGICDLYVKALDRRQFRVDVVHSGEAALEAVSRQD
                     YDFIFLDLKMPGFSGVDTFRELKARDVQSLIIIVTGYPDSELLVEAMRLGPLTVILKP
                     FDPAEIKKSVESLVQMTSK"
     gene            complement(1218..1553)
                     /gene="yajC"
                     /locus_tag="VFR02_08480"
     CDS             complement(1218..1553)
                     /gene="yajC"
                     /locus_tag="VFR02_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET9870515.1"
                     /translation="MDPVYAQTTAPAAGAPASLPPDSPLAGLFSMLPFILIAAVFYFL
                     ILRPQGKETKRREEMVKNLQRGDKVLTRGGLYGTVADLKENVVVLKVNENSKVEVNRS
                     YVESVEKAP"
     gene            complement(1642..3492)
                     /gene="glmS"
                     /locus_tag="VFR02_08485"
     CDS             complement(1642..3492)
                     /gene="glmS"
                     /locus_tag="VFR02_08485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9870516.1"
                     /translation="MCGIAGYTGPKPAIPILLGGLAKLEYRGYDSAGLALLTPAGKMR
                     ILKKAGKIGNLVAAVKKAGLDPSLHAGIGHTRWATHGHPTDQNAHPHTDCSGSIAVVH
                     NGIIENYQKLKSELTARGHRFKSQTDSEVLAHLIEEGLRKKSWEAAVRWALSRITGTY
                     SVVAVCEAAPRTLIGARSGGGALVVGLGQGENFIASDVPALLSHTQKVFYLDDDEMAV
                     VTPEGAAVVGIQDGKARAKEPTRVDWTAEQAEKGGYPHFMLKEIFEQPRALEDTLTGR
                     LDPRSGEVVLEGGFVRAPWAERFQKITLLACGTSYYAGLVGKFLLEEQLRVPVEVDYA
                     SEFRYRRPVLGPDHLAVVITQSGETVDTLVALREAKARGAKVVAICNVMGSSATREAD
                     AVLLTRAGPEIGVASTKAFTTQLEALWLLSLHLARVRRSLPPKALRQKGLALARIPTL
                     ATAALKQRKAVEKIARRFAPHYNFLYLGRGINYPIALEGALKLKEISYIHAEGYPGGE
                     MKHGPIALIDQRLPVVAIALRSSGVYEKMLNNMEEVKARSGKLLAVVEKGDKLAARKA
                     DAVLEIPPCPEDLSPILSVLPLQLLAYEIARLKGREIDQPRNLAKSVTVE"
BASE COUNT          697 a         1157 c         1186 g          692 t
ORIGIN      
        1 agccccgaac caggcacggg acaatccttc gggaaaaggg gcggggggcc cttgggtgac
       61 cgcatccagg aattcaagga cggcgtccgg cccgccgggg aaggaacggc cgtccagttc
      121 cgcggccggg tcccccgcca gcaggcacca gcgtcccgcg tcctccaggg ggccggcgct
      181 ttccaggaac accgccgcgc cccgggaagc cagggggccc cagcgttcca tccagggaaa
      241 ggcggtctcg aaaacgccgc gggccagggg gaccagttcg gggaaggccg ccgtcggcag
      301 gggatgggat ctcatggcgc cattgaaaca aaggccggcg ccgggcgcca gccctttccg
      361 gaccataaaa agcgtccggt ttcaggtgaa aaaaggggaa gaagctactt ggaggtcatc
      421 tggacgaggg attccacgga cttcttgatc tccgccggat cgaagggctt caggatgacc
      481 gtcaaggggc ccaggcgcat ggcctccacc aaaagctcac tgtccggata gcccgtcacg
      541 atgatgatga gggactgcac gtccctggcc ttgagttccc ggaaggtgtc gacgccgctg
      601 aaaccgggca tcttgaggtc gaggaagatg aagtcgtaat cctggcggga caccgcttcc
      661 agggcggctt cccccgagtg gaccacgtcc acccggaatt ggcggcggtc caaggccttg
      721 acgtagaggt cgcagatgcc cggctcgtcg tccaccacca acacgcgctt ctgtccggcc
      781 tgggcaccct tctccaacca gcggtcgatg agttccttct tgaagcgcca ctcgctgccg
      841 atcttgaacg agggcagttt tccctgttgg gccaatttgt agatggtggt ggtggtgatc
      901 ttgaggtagg cggagagctc ttgggcggtc aggatgtcgt tcataaaaga ggcccgttct
      961 tcatgggaaa tttaagattg caacatttat ggcgttcagc ttgggcaatt atatcccaag
     1021 tcgagggagg cctgtgcctt taattattaa atcgggggaa aaaatagttt cagacaaaca
     1081 ccggggaagg cgcgacgttg tttttcactt tattccggaa agcctaatat cgaccgggga
     1141 aaatcctttt ccccaagcgc caaacgccga gacaaggggg cgcggcccgg ggccggcccc
     1201 cggccaaacc gcacggttca gggtgctttt tcgaccgact cgacgtagga gcggttcact
     1261 tccaccttgc tgttctcgtt gaccttcagg accacgacat tttccttcag gtcggccacc
     1321 gtcccgtaga ggccgccccg ggtcagcacc ttgtcgcccc gttgcaggtt cttgaccatc
     1381 tcctcccggc gcttggtctc cttgccctgc ggccgcagga tgaggaaata gaagacggcc
     1441 gcgatcagga tgaagggcaa catggaaaag agcccggcga gcggcgagtc cggcgggagc
     1501 gacgcggggg cgccggcggc gggcgcggtg gtctgggcgt aaacgggatc catgaacagc
     1561 ctcctgggac ggggtgtcga cccgtcggat tgtatcgggt ttccccgtca ggtcaacccg
     1621 gaactggccc gcgcggggcc ctcattcaac ggtcacgctc ttggccaggt tgcggggttg
     1681 gtcgatttcc cggcccttga gccgggcgat ctcgtaggcc agcaactgca ggggcaacac
     1741 cgagaggatc ggcgagaggt cctccgggca gggggggatc tccagcaccg cgtcggcctt
     1801 tcgggcggcc agcttgtccc ccttctccac cacggccagc agcttccccg accgcgcctt
     1861 cacttcttcc atgttgttga gcatcttctc atagacgccg gaagatctca gggcgatggc
     1921 cacgaccggc aggcgctggt cgatgagcgc gatgggcccg tgcttcatct cgccgcccgg
     1981 gtagccctcg gcgtggatat aggagatctc cttgagtttc agcgcccctt ccaacgcgat
     2041 gggatagttg atcccccggc ccaggtaaag gaagttgtag tgcggggcga aacgccgcgc
     2101 gatcttttcc acggctttgc gctgcttgag ggccgcggtg gccagggtgg ggatccgggc
     2161 cagggccagg cccttttgcc gcagtgcctt cgggggcagc gaccgccgca cccgggccag
     2221 gtgcaggctc agcagccaca gcgcctccag ttgggtggtg aaggccttgg tggaggccac
     2281 gccgatctcg gggcccgccc gggtcagcag cacggcgtcc gcctcgcggg tggccgagct
     2341 tcccatgacg ttgcagatcg ccaccacctt ggcgccccgg gccttggcct cgcgcagggc
     2401 caccagggta tctaccgtct caccgctctg ggtgatgacc accgccaaat ggtccgggcc
     2461 caggaccgga cggcggtaac ggaactcgga agcgtaatcc acctcgacgg gcacgcgcag
     2521 ctgctcctcc agcaggaact tccccaccag gcccgcgtaa taggaagtgc cgcaggccag
     2581 cagggtgatc ttctggaacc gttcggccca aggcgccctg acgaacccgc cctccaggac
     2641 cacctcgccc gaacgcgggt ccaggcgtcc ggtcagggtg tcttccagcg cccggggttg
     2701 ttcgaagatc tccttgagca tgaagtgcgg gtagccgccc ttctcggcct gttcggcggt
     2761 ccagtcgacc cgcgtgggct ccttggcccg ggccttgccg tcctggatcc cgaccacggc
     2821 cgccccctcg ggggtcacca ccgccatctc gtcgtcgtcc aggtaaaaga ccttctgggt
     2881 atgggacagc agggccggca cgtcggaagc gatgaagttc tcgccttggc ccaggcccac
     2941 caccagggcg ccgccgcccg agcgggcgcc gatgagggtc cggggggcgg cctcgcagac
     3001 ggccaccacc gaataggtcc ccgtgatgcg ggaaagggcc cagcgcacgg ccgcctccca
     3061 gctttttttg cgcaggcctt cctcgatcaa atgggccagc acctcggagt cggtctggga
     3121 cttgaaacgg tgtccccggg ccgtcaattc ggacttgagc ttttggtagt tctcgatgat
     3181 gccgttgtgc accaccgcga tggagcccga acaatcggta tggggatggg cgttctggtc
     3241 cgtggggtgg ccatgggtgg cccagcgggt gtggccgatg ccggcatgga gcgaagggtc
     3301 caggcccgcc ttcttgacgg cggccaccag gtttccgatc ttgccggctt ttttcaggat
     3361 cctcatcttg cccgcgggcg tcagcagggc caacccggcc gaatcatagc cccggtattc
     3421 cagcttggcc aggccgccca gcaggatggg gatggcgggc ttggggcccg tataaccggc
     3481 gatgccgcac ataaaaacct caaaacaaaa gatttaaagg aatccatctg catagccaaa
     3541 cctaagtatc aggcaaacca aaatttgaaa atcaatctca tcaaccctca aaaattttat
     3601 tttttcactt tggattgctc gattatcttt gcccacaaat tgtttccagt ttttctggtt
     3661 tttctgttta aggccaaaaa caaaagagca agcaaagaaa aaagaactgt aagtacaata
     3721 agtactgact ta
//
LOCUS       DASTJJ010000699         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1685907, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000699 DASTJJ010000000
VERSION     DASTJJ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685907"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFR02_08490"
     CDS             <1..650
                     /locus_tag="VFR02_08490"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9870517.1"
                     /translation="GVGMNLGSTDPLDLPYFTRLKALVDRYQPEWVSDHLCWIGAGGL
                     QSHDLLPLPFTREALAHTASRIRRVQDLLGRRILIENLSGYLQFANAEMSEWEFVRGV
                     AEAADCDLLLDVNNVYVNSENLRFDPIEYLNAIPVERVKQMHLAGHLRSDGILIDDHG
                     SPVADEVWDLYRHAVGRFADVPTLIEWDLNIPEFDVLFAESKKAQAILGEKLHVA"
     gene            640..1323
                     /locus_tag="VFR02_08495"
     CDS             640..1323
                     /locus_tag="VFR02_08495"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9870518.1"
                     /translation="MWLENAQAEFIQYLYEPKPSGERGGRVRELFLSPDRPDPGNGLK
                     VYRRNLLFGMLQALKDTYGFTRALVGENNFNFLGREYIYANPSRHPDLTAYGGDFAAF
                     LRGRAEVAAWPFLADVAALEWARDRAFYAGPPVAEGVLAPSLSLVESAYGILGPFELF
                     QKGGLAALPQDPFRSGPEALVVWAEAGEPRQARVTRETAEVVRSLIGFQGPLAPEDLA
                     FLRTEWFSI"
     gene            1399..2118
                     /locus_tag="VFR02_08500"
     CDS             1399..2118
                     /locus_tag="VFR02_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870519.1"
                     /translation="MIFRLTPEGIVKRRRSIWIGGLYFAAFFSLIQFLLCHHSLADGS
                     LSPSGFWSLFSLMVGLPAGGLVMVMTLNLRKPDEVELDREALLRRRDGKMSEVPYASI
                     QEMKVAARSGRPSVIFLRAGKNRWTLHDYEDMPRLASGLEQALTPGNVETKEALPPNP
                     LRRAGMVGIFAGIGLLAALAVVLLIMIGSWTGSGIPILLLMLGISGFGKNRNHPTKRI
                     LAILSILAAVLWVALKFFADR"
     gene            2141..>2695
                     /locus_tag="VFR02_08505"
     CDS             2141..>2695
                     /locus_tag="VFR02_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HET9870520.1"
                     /translation="MKDASPRRYPALRAIWLSFYSRDLYRDVRSNWKGTGLGLLCAVF
                     ALLGVLLAARFQVWTAAEVPGFAEKIPPFQVAQGEFTSPVAQPYRIDYPKGCIMVDTT
                     GKFRSIDQVPGVEGFETAYLVTKDGYSVRQVRRGKVEDRYHPFKPTTHFAATRGDIDR
                     WTKALVRWAGPGLYVVYLPIFLPIE"
BASE COUNT          439 a          845 c          846 g          565 t
ORIGIN      
        1 acggcgtggg catgaacctg gggtccaccg accccctgga cctcccttat ttcacccgcc
       61 tgaaggccct cgttgaccgc taccagcccg aatgggtctc ggaccacctt tgctggatcg
      121 gcgccggggg cctgcaaagc cacgacctgc tgcccttgcc cttcacccgg gaggccctgg
      181 cgcacaccgc ctcgcgcatc cgccgggtcc aggacctcct gggccggcgc atcctcatcg
      241 agaacctgtc gggctacctc cagttcgcga acgccgagat gagcgagtgg gaattcgtgc
      301 ggggcgtggc cgaggcggcc gattgcgacc tgcttttgga cgtgaacaac gtttacgtca
      361 attcggagaa cctgcgtttc gaccccatcg agtatctcaa cgccatcccg gtggagcggg
      421 tcaaacagat gcacctggcc gggcacctgc gctcggacgg catcctgatc gatgaccatg
      481 gttcgccggt ggcggatgag gtctgggacc tttaccgcca cgcggtgggg cgcttcgccg
      541 acgtgcccac cctgatcgaa tgggacctga acatccccga gttcgacgtg ctcttcgccg
      601 aatccaaaaa ggcccaggcc atcttggggg agaaactgca tgtggcttga aaacgcccag
      661 gccgagttca tccaatacct ctacgaaccc aaaccctcgg gggagcgggg cggccgggtg
      721 cgggaacttt tcctttcccc cgacaggccc gacccgggga acgggttgaa ggtctaccga
      781 cgcaatctgc tcttcggcat gctgcaggcc ttgaaggaca cctacggctt cacccgggcg
      841 ctggtgggcg agaacaactt caatttcctg ggaagggaat atatctacgc gaacccttcc
      901 cggcacccgg acctgaccgc ctatggcggc gacttcgccg ctttcctgcg gggccgcgcc
      961 gaagtcgccg cctggccttt tttggccgac gtggccgcct tggaatgggc ccgggatcgg
     1021 gccttttacg cgggaccgcc cgtggcggaa ggggtgctgg cgccctccct gtccttggtc
     1081 gagagcgcct atgggatctt gggacccttt gagttgttcc aaaagggagg gttggcggcc
     1141 ctcccccaag accccttccg ttcgggcccg gaggccctgg tggtctgggc cgaggcgggc
     1201 gaaccgcggc aggcgagggt cacccgggaa accgcggagg tggtccgcag cctgatcggc
     1261 ttccaggggc ccctggctcc cgaagacctt gcgttcctcc gaacggaatg gttctcgatc
     1321 taggcggctg tttttccttt ttctgtccgg gttcctggtg catcctatgg cggtccatcc
     1381 acttttccag gagaaccagt gatcttccga ttgaccccgg aggggatcgt caaaaggcgc
     1441 cgttcgattt ggatcggcgg actttatttc gccgccttct tcagcctgat ccagttcctg
     1501 ctttgtcatc actcccttgc cgacgggtcc ctgagcccat cgggtttttg gtcccttttt
     1561 tcgctgatgg tgggtctccc ggcgggcggg cttgttatgg tcatgaccct gaatctccgg
     1621 aagccggatg aagtggagct ggatcgggaa gccttgctgc gccggaggga tggcaagatg
     1681 tccgaggtcc cttatgcctc gatccaggaa atgaaggttg cggctcggtc cggccgtcct
     1741 tcggtcattt tccttcgggc gggcaagaac cgttggaccc ttcacgatta cgaggacatg
     1801 ccccggttgg cgagtggatt ggagcaggcc ttgacgccgg ggaacgtgga aacgaaggaa
     1861 gccctcccgc cgaacccatt gcgtagggcc ggaatggtcg gcatcttcgc ggggatcggg
     1921 ttgctcgcgg ccctggcggt cgttctcctc atcatgatcg gttcctggac gggatcgggc
     1981 atcccgatcc ttcttttaat gttgggcatt tcgggtttcg ggaagaaccg gaaccatccc
     2041 accaagcgga tcctcgccat cctatccatc ctcgccgccg tcctttgggt ggcgctcaaa
     2101 tttttcgcgg accgctgacc gtcccaatgg aggaagcact gtgaaagatg cgtcaccccg
     2161 ccgttatccg gccctgcggg ccatctggct ttccttttat tccagggacc tttatcggga
     2221 tgtcaggtcc aactggaaag ggaccggact gggattgctc tgcgcggtct tcgcgctcct
     2281 gggcgttttg ttggccgcgc ggttccaggt ctggacggcc gcggaggtgc ccggattcgc
     2341 cgaaaagatc ccgcccttcc aggtggcgca gggggaattc accagtccgg tcgcacaacc
     2401 ctaccggatc gattacccca agggctgcat catggtggac accacgggaa aattccgctc
     2461 catcgatcag gtgccaggtg tcgaaggttt cgagaccgct tacttggtga ccaaggacgg
     2521 ttattccgtg cgccaggtgc ggcgggggaa ggtcgaggac cggtaccacc ccttcaaacc
     2581 gaccacccat ttcgccgcca cccgggggga catcgatcgg tggacgaagg cattggtccg
     2641 ctgggccggt ccgggtcttt acgtcgtcta tctgccgatc tttttgccga tcgag
//
LOCUS       DASTJJ010000700         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1441753, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000700 DASTJJ010000000
VERSION     DASTJJ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441753"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /gene="rplE"
                     /locus_tag="VFR02_08510"
     CDS             complement(<1..123)
                     /gene="rplE"
                     /locus_tag="VFR02_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274974.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9870521.1"
                     /translation="MPTTTILKEKYSKEIIPALMKKFDIKNAMRVPKMEKVVLNV"
     gene            complement(125..445)
                     /gene="rplX"
                     /locus_tag="VFR02_08515"
     CDS             complement(125..445)
                     /gene="rplX"
                     /locus_tag="VFR02_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9870522.1"
                     /translation="MKVHREDTVLVLTGKDKGKKGRVIRVFPKQDKVLVEKVNMVKRH
                     TRPTQQLPQGGIVEKELPVHVSNVQVVCPKCGKSTRVAHKILASGQKNRTCKKCGEIM
                     DKEK"
     gene            complement(449..817)
                     /gene="rplN"
                     /locus_tag="VFR02_08520"
     CDS             complement(449..817)
                     /gene="rplN"
                     /locus_tag="VFR02_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9870523.1"
                     /translation="MIMPQTLLNVADNSGATKIMCIHVLGGTRRRYAGIGDVIIAAVK
                     EAIPGAGVKKSEVVKAVVVRQRRVLVRPDGSNIRFDENAAVIINEQMNPRGTRIFGPV
                     TRELRDKNFMKIISLAPEVL"
     gene            complement(814..1107)
                     /gene="rpsQ"
                     /locus_tag="VFR02_08525"
     CDS             complement(814..1107)
                     /gene="rpsQ"
                     /locus_tag="VFR02_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9870524.1"
                     /translation="MSAETQPNRKEGRTKTAVVVSRNMDKTAVVAIERVVKHPLYQKL
                     VRRRSKFKVHDPKNETHKGDVVLVAETRPISGGKHWRLVKILRKAVQVEEAQV"
     gene            complement(1119..1319)
                     /gene="rpmC"
                     /locus_tag="VFR02_08530"
     CDS             complement(1119..1319)
                     /gene="rpmC"
                     /locus_tag="VFR02_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9870525.1"
                     /translation="MKSKEVREFNIEQLKQHLISKEEELFNLKIQLSTKQLENPMKIR
                     EARRNVARLKTILREKEKGTKA"
     gene            complement(1316..>1577)
                     /gene="rplP"
                     /locus_tag="VFR02_08535"
     CDS             complement(1316..>1577)
                     /gene="rplP"
                     /locus_tag="VFR02_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9870526.1"
                     /translation="RHMKRVGKLWVRIFPDRPVTRKPAEVRMGGGKGAPDHWAAVVKP
                     GRILFEVSGAPEAVVREAFQLAAHKLPVKTKTLSRADVGGMS"
BASE COUNT          229 a          513 c          462 g          373 t
ORIGIN      
        1 gacgttcaag accaccttct ccatcttggg cacccgcatg gcgttcttga tgtcgaattt
       61 cttcatcagg gccgggatga tctccttgga gtacttttcc ttcaggatcg tcgtggtcgg
      121 catcttactt ttccttgtcc atgatttcgc cgcacttctt gcaggtgcgg ttcttctggc
      181 ccgaggccag gatcttatgg gccacccggg tcgacttgcc gcacttgggg cagaccacct
      241 ggacgttcga cacgtggacc ggcagttcct tctccacgat gccgccctgg ggcagctgct
      301 gggtgggccg ggtatggcgc ttgaccatgt tgaccttctc caccaacacc ttgtcctgct
      361 tggggaacac gcggatcacg cggcccttct tgcccttgtc cttccccgtc aacaccaaaa
      421 ccgtatcctc acgatggact ttcatggttt acaggacctc cggcgccagc gagatgatct
      481 tcatgaagtt cttatcgcgc agttcgcggg tcacgggccc gaagatgcgg gtgccccggg
      541 ggttcatctg ctcgttgatg atgaccgcgg cgttctcgtc gaagcggatg ttggagccgt
      601 cgggccgcac cagcacgcgg cgctggcgga ccaccacggc cttcaccact tcgctcttct
      661 tcaccccggc gcccgggatg gcttccttga ccgcggcgat gatgacgtcg ccgatccccg
      721 cgtaacgacg gcgggtgccg cccaacacgt ggatgcacat gatcttcgtc gcgccgctgt
      781 tgtcggcgac gttcaggagg gtctggggca tgatcatact tgggcctcct cgacctggac
      841 ggccttgcgc aggatcttca ccaggcgcca gtgcttgccg ccgctgatcg gccgggtctc
      901 ggccaccagc accacgtcgc ccttgtgggt ctcgttcttg gggtcgtgga ccttgaactt
      961 gctgcgccgc cgcaccagct tctggtacag ggggtgcttg accacgcgct cgatggcgac
     1021 cacggcggtc ttgtccatgt tgcggctgac cacgacggcc gtcttggtcc ggccttcctt
     1081 acgattgggt tgggtctcgg ctgacatcaa acgctccttt atgctttcgt gcctttttct
     1141 ttttcccgga ggatcgtctt caggcgcgcc acgttgcggc gcgcttcccg gatcttcatc
     1201 gggttctcca actgcttggt ggagagctgg atcttcaggt tgaacagctc ctcttccttg
     1261 gagatgaggt gctgtttcaa ttgctcgata ttgaactcgc ggacttcctt ggatttcatg
     1321 acatccctcc cacatcggcc cgggatagag tcttcgtctt caccgggagt ttatgcgccg
     1381 ccaactgaaa ggcttcccgc accacggctt ccggggcgcc ggacacctcg aacaggatgc
     1441 ggcccggctt gaccaccgcg gcccaatggt ccggggcgcc cttaccgccg cccatacgca
     1501 cttcggcggg cttgcgggtc accggacggt cggggaaaat gcgcacccac agcttgccca
     1561 cccgcttcat gtggcgg
//
LOCUS       DASTJJ010000701         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_954076, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000701 DASTJJ010000000
VERSION     DASTJJ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954076"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFR02_08540"
     CDS             complement(<1..229)
                     /locus_tag="VFR02_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9870527.1"
                     /translation="MAEMLGGWKRSHKCGDLRASDVGKTVTLMGWVHHRRDHGGLVFV
                     DLRDRYGVTQIVFDPQLADKVHKQSHLIRNEW"
     gene            complement(367..1431)
                     /locus_tag="VFR02_08545"
     CDS             complement(367..1431)
                     /locus_tag="VFR02_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9870528.1"
                     /translation="MGLRFDMTVPLARYYANHANDLPRPFRCSHIGPVWRADRPQRGR
                     FREFYQCDIDIVGGASDSYEVELLLATDRVLKALGVGKFKIRISDKSLLTAVLRRIGT
                     PVEKLAGVAVAIDKMDKLSFEDVKKEIGGLGLEGENFEKIVLFLNSAKSGFLDLSLFK
                     EVKINELGDRIKSIQDRVKAVEPGANVVFDPLLVRGFDYYTGPVFEINVEDKDFSFSI
                     GGGGRYDGLIEKLGGPKGTPAVGLSLGFERILTILMEKDQAGSQAPASRVLLANHGQP
                     EKDILALAEKLRSAGIQVETVLEPKKLGQQLEAAQKAGIEFAVTAHAPGSETLKVRRL
                     STREDKEMGLEELKGLAGKP"
BASE COUNT          286 a          521 c          432 g          304 t
ORIGIN      
        1 cccattcgtt gcggatgagg tgggactgct tatgcacctt gtcggccaac tgggggtcga
       61 acacgatctg ggtcacgccg tagcggtcgc gcaggtccac gaagaccaac cccccgtggt
      121 cgcggcggtg gtgcacccaa cccatgaggg tcacggtctt gcccacgtcg gaggcccgca
      181 ggtcgccgca cttatgggat cgtttccaac cgccgagcat ttccgccact ttcattcccc
      241 ctgaacattt tcaccacgag cccctgacgg ggccacgaag gacacgaagt tcaaacacgc
      301 ctcaaaacaa aaaggcccct aaaaaggagc cgtataataa cacaatcttc catttccaaa
      361 aaacttctaa ggcttccccg ccaacccctt gagttcctcc aggcccatct ccttgtcttc
      421 ccgcgtggac agccgccgca ccttgagggt ttccgagccc ggcgcatggg ccgtcacggc
      481 gaactcgatg cccgccttct gggcggcctc caactgctgg cccagctttt tgggttccaa
      541 gaccgtctcc acctggatgc ccgccgaacg cagcttctcg gccaaggcca ggatgtcctt
      601 ctcgggctgg ccgtggttgg ccaagagcac ccgggaggcg ggcgcctggc tgcccgcctg
      661 gtccttttcc atcaggatgg tcaggatgcg ctcgaagccc agcgacagcc ccacggcggg
      721 agtgcccttg gggccgccca gcttctcgat gagcccgtcg tagcgtccgc cgccgccgat
      781 ggagaagctg aagtccttgt cctcgacgtt gatctcgaag accgggcccg tgtaatagtc
      841 gaaaccgcgc accaagagcg ggtcgaagac cacgttggcc cccggttcga cggccttcac
      901 ccggtcctgg atggacttga tccggtcgcc cagctcgttg atcttcactt ctttaaagag
      961 ggacaggtcc aggaaaccgc tcttggcgct gttgaggaaa agcacgatct tctcgaaatt
     1021 ctcgccttcc aggcccaggc cgccgatctc cttcttcacg tcctcgaagg acagcttgtc
     1081 catcttgtcg atggccaccg ccacgcccgc cagcttttcc accggcgtgc cgatgcgccg
     1141 cagcaccgcc gtcaagaggg acttgtcgct gatgcggatc ttgaacttcc ccaccccgag
     1201 cgccttcagc acccggtcgg tggcgagcag cagctccacc tcataactgt cggaggcgcc
     1261 gcccacgatg tcgatgtcgc attggtagaa ttcgcggaaa cggccccgct ggggccgatc
     1321 cgcccgccag acgggtccaa tgtgggaaca gcggaagggc cggggcaggt cgttggcgtg
     1381 gttggcgtaa tagcgcgcca ggggcacggt catgtcgaac cgcagaccca tgtcggccaa
     1441 tgtaatcaac cgactcttcc ctcgcgactc aatcgaaagc gcttccgcac cttccgatga
     1501 ataaagcgtc gtttctttta tttcatctcc attttgggct tca
//
LOCUS       DASTJJ010000702         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1686350, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000702 DASTJJ010000000
VERSION     DASTJJ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686350"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFR02_08550"
     CDS             complement(<1..332)
                     /locus_tag="VFR02_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870529.1"
                     /translation="MFRDYFQQIKLAYTRPGRFFEQALQQGSLSQALRFSALTGVCVA
                     LELGVSEAFASSSLPIVGLVTALTLVALPLASVVWVFGWAAFIRLCGFLLQEDLASPS
                     LRLMVGYSS"
     gene            complement(401..790)
                     /locus_tag="VFR02_08555"
     CDS             complement(401..790)
                     /locus_tag="VFR02_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870530.1"
                     /translation="MARKSDHDEDELEDLEDDFEDDDEDGDDEDDTIDLDSAEEEEED
                     DDDDEDDEEDDSVESIEARRLFQSEAEEILEHLTLDDVTEVLRENDLEVGDAPKLRRF
                     LQEVVAEGEVTTMEEAWEEALTRLEES"
     gene            949..1077
                     /locus_tag="VFR02_08560"
                     /pseudo
     CDS             949..1077
                     /locus_tag="VFR02_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460921.1"
                     /note="functions in degradation of stringent response
                     intracellular messenger ppGpp; in Escherichia coli this
                     gene is co-transcribed with the toxin/antitoxin genes
                     mazEF; activity of MazG is inhibited by MazEF in vitro;
                     ppGpp inhibits mazEF expression; MazG thus works in
                     limiting the toxic activity of the MazF toxin induced
                     during starvation; MazG also interacts with the GTPase
                     protein Era; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
BASE COUNT          217 a          395 c          366 g          223 t
ORIGIN      
        1 gaagaatagc ccaccatgag ccgcagggaa ggcgaggcca ggtcttcctg gagcaggaag
       61 ccgcacaacc ggatgaaggc cgcccaaccg aagacccaga ccaccgaagc caggggaagg
      121 gccaccaggg tcaacgcggt caccaatccg acgatgggca gcgagctgct ggcgaaggcc
      181 tctgaaacgc ccaactcgag cgccacgcag acgcccgtca gggcggaaaa acgcagggct
      241 tgggacaggc ttccctgttg cagggcctgc tcaaaaaaac ggcccgggcg ggtgtaggcc
      301 agcttgatct gttgaaaata gtcgcgaaac atgggctaga gtctagcaca aagggctggc
      361 gcgggggcct tttccgcccc cgggcccggc gcccgggggg tcaggattcc tccaggcggg
      421 tcaaggcctc ttcccaggcc tcttccatgg tggtgacttc cccctcggcg accacttcct
      481 gcaggaagcg cctcagcttg ggggcgtccc cgacctccag gtcgttctcc cgcagcactt
      541 cggtgacgtc gtccagggtc aggtgttcca ggatctcctc ggcctcgctt tggaaaaggc
      601 gccgggcttc gatgctctcg accgaatcgt cttcctcatc gtcctcgtcg tcatcgtcgt
      661 cctcttcctc ttcctcggcg ctgtccaggt cgatggtgtc gtcctcgtcg tcgccgtcct
      721 cgtcgtcgtc ctcgaaatcg tcctccagat cctccaactc atcttcgtcg tgatcgcttt
      781 tacgggccaa aacggacctc ccggtcggaa tgaatcagca aggatgccct tcgccccaat
      841 gaatacttcg acttgatttg gatgtcaagt gtttcggccc gcggccgggg gcgcggttgg
      901 cgcccctggg cgcttcatgc taaagtccat gcgaccggga ggccgacctt ggaccaattg
      961 aaacgtcttg aaaaattgat ggcccgcctg cggggtccgg gcggctgtcc ttgggaccgc
     1021 cgccagaccc accggagctt gaagccctat ctcttggaag aggccgggga agaggccttg
     1081 ccccgcctgg aggaagcctg accccccggg cgccgggccc gggggcggaa aaggcccccg
     1141 cgccagccct ttgtgctaga ctctagccca tgtttcgcga ctattttcaa cagatcaagc
     1201 t
//
LOCUS       DASTJJ010000703         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1319917, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000703 DASTJJ010000000
VERSION     DASTJJ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319917"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFR02_08565"
     CDS             complement(<1..615)
                     /locus_tag="VFR02_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870531.1"
                     /translation="MVLTGLFLGCTRQIAVNPTTPGGVNTKTFTPVPPTNTFTVTLTP
                     TVTPTPNGPTNTFTPTGTPTVTRTVTNTATPTLTVTATSTATPDPNMIDDFEDNDGNI
                     IAQGGRNGFWYTANDASSTINPSTFLPTADTSDGSQYVGNVSGTVAASGFAELAASFS
                     NPKVAYAIPGGATGVIFDVKATGPSSIWFSISDTYTDANGSDADG"
     gene            complement(943..>2586)
                     /locus_tag="VFR02_08570"
     CDS             complement(943..>2586)
                     /locus_tag="VFR02_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870532.1"
                     /translation="QTGKLVDLLQTVAAWDPTNIEAREKLTELLMEAKQNEAAISSLQ
                     GLVEIYISKNELDKAEKTAQRSIALGDVETYYHLGVIFFNQQKFEEARSAFETFLSKQ
                     ETHVGALKYLAMAYTRLGQVPQAVGVYLKILNVYFSENLLDEARDIQHTILEMDPGNE
                     DVKQYAQGLNPAPVEEAPPAEESPEPPPPAEGEAGAEPDDEASRQAMLAQAASYSDKG
                     LYEQAIDVYLDMLKRWPEWPEIRARLQQAYALMARAAEPAEKGPSPETIKAELERELK
                     EQMRREMEEQSRRLQEEQKRLNQERHFEAEMKRQLEEMRLKQDLESKLLEQVQRTKED
                     ELRQRIAKEFEEKQRQLTEERDRLEREKLESINRIKDELEQTKQSYEQKIRQQVEKEM
                     QERLRAEVSEKERQEKEGIRQVQLQAALHQEDLERNRFEAQKKANEAARAKVDQEISQ
                     GMERLRQEKEKEKKSQPAAPPPAAPAPPAAGAPAVTPAAPGGDSLEDPFIRQTLADIY
                     AKQGLFVEALKIYERILNEDPDNQDVKDKLRNILKMKGI"
BASE COUNT          374 a          777 c          844 g          591 t
ORIGIN      
        1 cccgtcggcg tcgctgccgt tggcgtcggt gtaagtgtcc gagatgctga accagatcga
       61 actggggccg gtggccttaa cgtcaaagat gacgcccgta gcgccgccgg ggatggcgta
      121 agccaccttg gggttgctga agctggcggc caattcggcg aagcccgagg cggccactgt
      181 cccgctcacg ttcccgacgt attggctccc atcgctggtg tccgcggtcg gaaggaaggt
      241 ggaaggattg atcgtggacg aggcatcgtt ggcggtgtac caaaagccgt tccggccgcc
      301 ctgcgcgatg atgttgccgt cattgtcctc aaaatcatcg atcatgttcg gatcgggcgt
      361 ggcggtggac gtggccgtta cggtcaaggt cggggtggcg gtgttggtca ccgtcctggt
      421 cacggtcggc gtgcccgtcg gggtgaaggt gttggtgggc ccgttcgggg tcggcgtgac
      481 cgtcggggtc agcgtcacgg tgaaggtgtt cgtcggggga acgggggtga aggtcttggt
      541 gttgaccccg cccggagtgg tggggttgac cgcgatctgg cgggtacagc ccaggaataa
      601 accggttaaa accatgccgg taaaggtgaa tccgaccgcc agtcgacgcc aaataaccat
      661 gagaacaacc ccctgggttt tagttacagg atgttagggc aaaatggatg ccacaaagcc
      721 aatgccctaa attaccacaa tccggtcttt tttcaataat aacggcccta aaaacggttt
      781 aaaaaaatgg gctcgtgtca caaatcaagg caaaatcgtg gggattctga cgtccagggg
      841 ttcggtcaat gaaagggatc gcgggcgcgg ccgacatggg gcttgtcttc cggccccgat
      901 gggtggacca ggtcacccgg tttcgctgga aagcgacgcg ggctatatcc ccttcatctt
      961 caggatgttc cgcagcttgt ccttcacatc ctggttgtcg ggatcctcgt tcaagattcg
     1021 ttcatagatc ttcagggctt ccacgaaaag cccctgtttg gcgtagatat cggccagggt
     1081 ctggcggatg aaggggtctt ccagcgaatc ccctcccggg gcggccgggg tgacggcggg
     1141 cgcgcccgcg gccggaggcg ccggcgcggc ggggggaggc gcggcgggtt gtgacttctt
     1201 ctctttttcc ttttcctgac gcaggcgttc catgccttgg gagatctcct ggtccacctt
     1261 ggcccgggcg gcctcattgg cctttttctg ggcctcgaac cggttgcgtt ccaggtcttc
     1321 ctggtggagc gcggcctgca gttggacctg ccggatcccc tctttttcct ggcgttcctt
     1381 ctcggagacc tcggcgcgga gcctttcctg catctccttc tcgacctgtt ggcggatctt
     1441 ctgttcgtag gactgcttgg tctgttccag ttcgtccttg atgcggttga tggactcgag
     1501 cttttcccgt tccaggcggt cccgctcctc cgtcaactgg cgctgttttt cctcgaactc
     1561 cttggcgatc cgctggcgca gttcatcttc cttggtccgc tggacctgct ccagcagctt
     1621 ggactccagg tcctgcttga ggcgcatctc ctccaactgc cgcttcatct cggcctcgaa
     1681 gtggcgttcc tggttcagcc gcttctgttc ctcctgcagc cggcgggact gctcctccat
     1741 ttcccggcgc atctgctcct tcagttcccg ctccagctcg gccttgatcg tctcgggcga
     1801 ggggcccttt tcggcgggct cggcggcgcg ggccatgagg gcataggcct gctggagccg
     1861 cgcgcggatc tcgggccatt cgggccagcg tttgagcatg tccaggtaga cgtcgatggc
     1921 ctgctcgtag aggcccttgt ccgaatagga cgccgcctgc gccagcatgg cctgccggga
     1981 cgcctcgtcg tcgggttcgg ccccggcctc cccttccgcc gggggcgggg gttcggggct
     2041 ttcctcggcc ggcggcgcct cctccacggg ggcggggttg aggccctggg cgtattgctt
     2101 gacgtcctcg ttccccgggt ccatctccag gatggtgtgc tggatgtccc gggcctcgtc
     2161 caggaggttc tcgctgaaat acacgttcag gatcttcagg tagaccccca cggcctgggg
     2221 cacctgcccc agccgcgtgt aggccatggc caggtacttg agcgcgccca cgtgggtttc
     2281 ctgtttagaa aggaaggtct cgaaggccga ccgcgcctcc tcgaacttct gctggttgaa
     2341 gaagatcacg cccaggtggt agtaggtctc cacgtcgccc agggcgatgg aacgttgggc
     2401 cgtcttctcg gccttgtcca gctcgttctt gctgatgtag atctccacca ggccctgcag
     2461 gctggaaatg gcggcctcgt tctgcttggc ctccatgagc agctcggtga gcttttcccg
     2521 ggcctcgatg ttggtggggt cccaggcggc cacggtctgg agcaggtcca ccaacttgcc
     2581 cgtctg
//
LOCUS       DASTJJ010000704         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_707712, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000704 DASTJJ010000000
VERSION     DASTJJ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707712"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="aroE"
                     /locus_tag="VFR02_08575"
     CDS             <1..859
                     /gene="aroE"
                     /locus_tag="VFR02_08575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET9870533.1"
                     /translation="MAAFPQKPAVNRTVFLIGKPLGHSLSPVMQNAAFRALGMRTYYA
                     PLELEPGQLPGFFTAFKSVNVVGANVTVPYKEKVLGRLDRVEKDAAWLGSVNTLYKKQ
                     GAVWGASTDGEGFLLSLGRRRAALRGSSGVLLGAGGAARSVAGALAGSGVKRLMIANR
                     SPDRARRLGRALIRRFPRMEVGMVSIAEAEKLLPRMDWVVQATSLGLEPGDPSPMSLR
                     GAGNSLWVADLVYHRTTAFLSQARRRRLWALGGVGMLLHQGALAFEKWTGQRAPLSVM
                     RSALLAGLR"
     gene            989..>1810
                     /locus_tag="VFR02_08580"
     CDS             989..>1810
                     /locus_tag="VFR02_08580"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9870534.1"
                     /translation="MKKRLGEMLVDEGIITEEQLQEALHEQKIKGGRLEKILISQGYV
                     TQDVIMAFVGTQLGIPHVSLAEIGSIPNDVVFSVPESIAINHVLIPIVRKDKKLTVAM
                     ADPLNVFAIDDIKMMTGFEVEPAIAGEAEIKAMQAKYYGSAAAGGAGEAGADMQEIIG
                     SMGGDSNLEVMEEKQEEIDISKLEAAGEDAPVVRLVNLILTEAVRSGASDIHIEPYEK
                     SLRCRYRIDGVLHEVQSPPKSISAAMSSRIKIMSSLDIAERRLPQDGRIMLRVQGK"
BASE COUNT          328 a          547 c          588 g          347 t
ORIGIN      
        1 catggcggct ttcccccaaa aaccggccgt caatcgaacg gtcttcctca tcggcaaacc
       61 cctcggccac tccctttccc ccgtcatgca gaacgccgcc tttcgggcgt tgggaatgcg
      121 gacctattac gcccccttgg aactggagcc aggacaattg cccgggttct tcacggcctt
      181 caagagcgtc aatgttgtgg gtgccaacgt caccgtcccc tacaaagaga aggtcttggg
      241 gcgtttggac cgggtggaga aggacgcggc ctggttggga tcggtcaata ccctctataa
      301 gaaacagggg gcggtctggg gagcttccac ggatggggaa ggattcctgc tttccctggg
      361 ccggaggcgg gcggccctgc gcggaagttc gggggttttg ttgggggcgg gcggcgcggc
      421 cagatccgtg gccggggccc tcgcgggatc cggggtgaag cggttgatga tcgccaaccg
      481 ttcccccgac cgggcccgcc gtttggggag ggccctgatc cgtcggttcc cccggatgga
      541 ggtgggcatg gtctcgatcg ccgaggcgga aaagctttta ccccggatgg actgggtggt
      601 ccaggccact tccctggggt tggaaccggg agatccctct ccgatgtcgt tgcgcggggc
      661 cgggaattcc ctttgggtgg ccgatctggt ctatcaccgc acgaccgctt tcttaagcca
      721 ggcccgccgt cgtcgtttat gggccttggg aggggtcggg atgcttttgc accagggagc
      781 tttggcgttc gaaaagtgga ccggccagcg ggctcctttg tcggtgatgc ggtccgcgct
      841 tctggcgggg ctccgataaa agggccgttt ttacaagcgt tgacttcaat ttttgcagac
      901 tctatactcc ctgagcgggg gttttgtcct gaaaccccga ggtccgttcg atttccttcc
      961 ttaaccgagg tccggaatgc ctgaatccat gaaaaagcgc ctgggtgaga tgttggtcga
     1021 cgagggcatc atcaccgagg agcagcttca ggaggccctt cacgagcaga agatcaaggg
     1081 cggccgcctc gagaagatcc tgatcagcca gggttatgtc acccaggatg tcatcatggc
     1141 cttcgtgggc acccagctgg gcatccccca tgtgagcctc gccgagatcg gttccatccc
     1201 caatgacgtg gtcttttctg tccccgaatc catcgccatc aaccacgtgc tcatccccat
     1261 cgtgcgcaag gacaagaagc tcacggtggc catggccgac cccctgaacg tcttcgccat
     1321 cgacgacatc aagatgatga ccgggttcga ggtggagccg gccatcgccg gcgaggccga
     1381 gatcaaggcc atgcaggcca agtattacgg cagcgcggcg gcgggtgggg ccggcgaagc
     1441 cggggccgac atgcaggaga tcatcggttc catgggcggg gacagcaacc tggaggtcat
     1501 ggaggaaaaa caggaggaga tcgacatctc caaactggag gcggccggcg aggacgcccc
     1561 cgtggtgcgc ctagtcaacc tcatcctgac cgaggcggtc cggtccgggg ccagcgacat
     1621 ccacattgaa ccctacgaga agagcctgcg ctgccggtac cgcatcgacg gcgtgctcca
     1681 cgaggtccaa tccccgccca aatccatctc ggcggccatg tcgtcccgca tcaagatcat
     1741 gtcctccctg gatatcgccg agcggcgcct gccgcaggac ggccgcatca tgttgcgggt
     1801 acagggcaag
//
LOCUS       DASTJJ010000705         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_829694, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000705 DASTJJ010000000
VERSION     DASTJJ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829694"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2244
                     /gene="dnaE"
                     /locus_tag="VFR02_08585"
     CDS             <1..>2244
                     /gene="dnaE"
                     /locus_tag="VFR02_08585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9870535.1"
                     /translation="SGCSDGEIADYLLKDEAPPALRAVGEYQDIFGKDRFFLEVQDHG
                     LAKEAKLRKALVELSRSSGVPLVATQGVHYLRPEEAGAHEVLLCLGKGEALSSPTHPS
                     YGSDQFYLKSPEEMAALFAEMPDALKRTLEISERCHLALTFDKLQMPQFPVPAGAGNE
                     DAYLGELCRLGLDRRFGDRASSQAVQDRLQEELDVIRRTGFSGYFLIVADFIQAAKDK
                     GIPVGPGRGSAAGSLVSYLLGITDVDPLRYGLLFERFINPERVSAPDIDVDVCDRRRG
                     EVLDYITQKYGADRVASIITFGTLAARAVLRDVGRVLEVPPAEIDRIAKLVPYELKIT
                     LEDCAAKVPELKAIATEPGPYRKLWDTARSLEGLYRHASTHAAGLIIGNGPLVEAVPL
                     YRGGEGEVLTQYDMTALKEVGLLKLDVLGLRTLTVIDDALELIARAGGKRPDLSRVPE
                     DDGPTFDLLRQARTGGIFQLESRGMRDYMRKLAPGNLEDITALIALYRPGPLGSDMVD
                     DFIRRKRGQVKFKYLHPRLEPILQNTYGVILYQEQVMRIAKDLAGFSLGQADLLRKSM
                     GSKDPEQMEKMRGRFLEGARAEGIPEETAESLFNLMAKFAGYGFNKSHSAAYARVAYQ
                     TAYLKANYPAQFMAALLTSESGNQDKVAQYVGECRRMGLKVLPPDVNESSESFTVDAK
                     GNIRFSLFAIKTVGGPSVSAILEARKAGPFTSLFDFCRRVDLKSMTPKLVESLILAGA
                     FDSTGAA"
BASE COUNT          366 a          787 c          749 g          342 t
ORIGIN      
        1 tttcgggctg ttcggacggc gagatcgccg actacctgct caaggacgag gcccccccgg
       61 ccctgcgggc ggtgggggaa taccaggaca tcttcggcaa ggaccgtttt ttcctcgagg
      121 ttcaggacca cgggctggcg aaggaggcca agctgcgcaa ggccctggtg gagctttccc
      181 gctcctcggg ggtgcccctg gtggccaccc agggcgtgca ttacctgcgg cccgaggagg
      241 ccggggccca cgaggtgctc ttatgcctgg gcaaggggga ggcgctttcc agccccaccc
      301 acccctccta cgggtcggac cagttctacc tgaagtcgcc cgaggagatg gcggccctct
      361 tcgccgagat gcccgacgcc ctcaagcgca ccctggaaat ttccgagcgc tgccacctgg
      421 ccctcacttt cgacaagctc cagatgcccc agttccccgt gccggcgggc gccggcaacg
      481 aggacgccta cctgggcgag ctctgccgcc tgggcctgga ccgccgcttc ggggaccggg
      541 cctcgtccca ggcggtgcag gaccggttgc aggaagagct ggacgtcatc cggcgcacgg
      601 gcttcagcgg ttatttcctc atcgtggccg acttcatcca ggccgccaag gacaagggga
      661 tccccgtggg tccgggccgg ggaagcgcgg cgggcagcct ggtgtcctac ctgttgggga
      721 tcacggacgt ggacccgttg cggtacggcc tgctcttcga acgcttcatc aaccccgagc
      781 gcgtcagcgc gcccgacatc gacgtggacg tctgcgaccg ccgccgcggc gaagtgctgg
      841 actacatcac ccagaagtac ggcgccgacc gggtggcttc catcatcacc ttcggcaccc
      901 tggcggcgcg ggcggtgctg cgcgacgtgg gccgggtgct ggaggtgccc cccgcggaga
      961 tcgaccgcat cgccaagctg gtgccctatg agctcaagat caccctggag gactgcgcgg
     1021 ccaaggtgcc cgagctcaag gccatcgcca ccgaaccggg cccctaccgc aagctctggg
     1081 acaccgcccg gtcgctggag ggcctctacc gccacgcctc cacccacgcg gccgggctca
     1141 tcatcggcaa cgggcccctg gtggaggccg tcccgctcta ccggggcggc gagggcgagg
     1201 tgctcaccca gtacgacatg acggccctga aggaagtggg cctgctcaaa ctggacgtgc
     1261 tgggcctgcg aaccctcacg gtcatcgacg acgccctgga gctcatcgcc cgggccgggg
     1321 gcaagcggcc cgacctttcc cgcgtgcccg aggacgacgg gcccaccttc gaccttttgc
     1381 gccaggcccg cacgggcggc atcttccagc tggagtcccg gggcatgcgg gactacatgc
     1441 gcaagctcgc ccccggcaac ctggaggaca tcacggcgct catcgccctg taccggcccg
     1501 gccccctggg ctcggacatg gtggacgact tcatccgccg caagcggggc caggtgaagt
     1561 tcaagtacct ccatccccgg ttggaaccca tcctgcagaa cacctacggg gtcatcctgt
     1621 accaggagca ggtcatgcgg atcgccaagg acctggcggg cttcagcctg gggcaggccg
     1681 acctcctgcg caaatccatg ggcagcaagg accccgagca gatggaaaag atgcggggcc
     1741 ggttcctgga gggcgcccgg gccgagggca tcccggagga gaccgccgag tccctcttca
     1801 acctgatggc caagttcgcg ggctacggct tcaacaagtc ccacagcgcg gcctacgcgc
     1861 gggtggccta ccagaccgcc tacctgaagg ccaactaccc ggcccagttc atggccgcgc
     1921 tcttgaccag cgagtcgggc aaccaggaca aggtggccca atacgtgggc gagtgccgcc
     1981 gcatggggct gaaggtgctg ccgccggacg tgaacgagag ctcggaaagc ttcacggtgg
     2041 atgcgaaggg gaacatccgc ttctccctct tcgccatcaa gaccgtgggc gggccctcgg
     2101 tctcggccat cttggaggcg cgcaaggccg ggcccttcac gtcgctcttc gacttctgcc
     2161 gccgggtgga cctcaagtcc atgacgccca agctggtgga aagcctcatc ctggcggggg
     2221 cctttgattc cacgggggcg gccc
//
LOCUS       DASTJJ010000706         1704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_585420, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000706 DASTJJ010000000
VERSION     DASTJJ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585420"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFR02_08590"
     CDS             complement(<1..795)
                     /locus_tag="VFR02_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9870536.1"
                     /translation="MSTILLVDDKSSMRKVLKQTLDGGGHSILEAEDGEKAMEMIKSK
                     HVDVVITDIKMPKVDGMTLLRMIKEIDGEIIVLIMTAYGTIETAVEAMKLGAYDYITK
                     PFSTEQVKITVDKAIERQKLFYENKYLREKLNDQYNYKRIVGNSGAMQPVYELIEKVA
                     PTDTAVLIRGESGTGKELVAHAIHFNSKRKDKPFIKVNCAVLAEGVLESELFGHEKGS
                     FTGAAGKRIGRFELAHGGSIFLDEIGDISLATQVKLLRVLQEKEFER"
     gene            complement(792..>1704)
                     /locus_tag="VFR02_08595"
     CDS             complement(792..>1704)
                     /locus_tag="VFR02_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870537.1"
                     /translation="GAPAEREIYLSEDPPKILRVRRAPVTGADAKVIGTEVLFTDETQ
                     LRSLESRIKVAEKMATIGELAAGIAHEIRNPLAAMKGFTEILQKKVKDNDSAREIVTD
                     IASEIEILNKIVTNFLVFAKPTAVETHEVELSELIRGLLPLVRKEAEAKRLRVSFRVG
                     EEAVLSLDSEQFRRALLNILLNAVQASPAKGAIDIGLERFSRKELAAWRAGFDFSPLL
                     LNETVNDWACVTVRDHGPGLAPEALKRLFTPFFTTKTEGFGLGLSITKKILEAHGGDV
                     GAAGHPEGGALFALFLPVIPTTNGGGQ"
BASE COUNT          262 a          558 c          529 g          355 t
ORIGIN      
        1 ccgctcgaac tccttttcct gcagcacccg cagcagcttc acctgggtgg ccaggctgat
       61 gtccccgatc tcgtccagga agatggaccc gccgtgggcc agctcgaacc ggccgatgcg
      121 cttgcccgcg gcgcccgtga aggagccctt ctcatggccg aacagctcgc tttccagcac
      181 gccctcggcc agcacggcgc aattgacctt gatgaagggc ttgtccttgc gtttgctgtt
      241 gaagtggatg gcgtgggcca ccagttcctt ccccgtgccc gattcgcccc ggatgagcac
      301 cgcggtgtcc gtgggggcca ccttctcgat gagctcatag acgggctgca tggcgccgct
      361 gttgcccacg atgcgcttgt agttgtactg gtcgttgagc ttttcccgca ggtatttgtt
      421 ctcgtagaac agcttctggc gctcgatggc cttatccacc gtgatcttga cctgttcggt
      481 ggaaaagggc ttggtgatgt agtcgtaggc gcccagtttc atggcctcca cggcggtctc
      541 gatggtgccg taggcggtca tgatgagcac gatgatctcc ccgtcgatct ccttgatcat
      601 gcgcagcagg gtcatgccgt ccaccttggg catcttgatg tccgtgatga ccacgtccac
      661 gtgcttggac ttgatcatct ccatggcctt ctcgccgtcc tcggcctcca ggatggaatg
      721 ccccccgccg tccagggtct gcttcaagac cttgcgcatg ctggacttgt cgtccaccaa
      781 taggatggtg ctcattgtcc gcctccgttc gtcgtaggga tgaccggcag gaacagggcg
      841 aacaacgccc cgccctccgg gtgcccggcc gcgcccacgt cgccgccgtg cgcctccagg
      901 atcttcttgg tgatggaaag cccgagcccg aaaccctcgg tcttggtggt gaaaaagggc
      961 gtgaagaggc gtttgagggc ctccggggcc agtccggggc cgtggtcccg gaccgtcacg
     1021 caggcccaat cgttcacggt ctcattgagc aatagggggg aaaagtcgaa ccccgcccgc
     1081 caggccgcca gttccttgcg gctgaaccgt tccaggccga tgtcgatggc gcccttggcg
     1141 ggcgaggcct ggaccgcgtt caacaggatg ttcaagaggg cccggcggaa ctgttcggaa
     1201 tccagggaaa gcacggcctc ctcccccacc cggaacgaca cgcgcagccg cttggcctcc
     1261 gcctccttgc gcaccagggg caacaacccc cggatgagct cggacaactc gacctcatgg
     1321 gtctccacgg ccgtgggctt ggcgaagacc aggaaattgg tcacgatctt gttcaggatc
     1381 tcgatctccg aggcgatgtc cgtcacgatc tcccgcgccg agtcgttgtc cttgaccttc
     1441 ttttggagga tctcggtgaa tcctttcatg gcggccaggg ggttgcggat ctcgtgggcg
     1501 atgccggcgg ccaattcgcc gatggtggcc atcttctcgg ccaccttgat gcggctttcc
     1561 aggctgcgca gctgggtctc gtcggtgaag aggacctcgg tgccgatgac cttggcgtcg
     1621 gcccccgtca cgggcgcccg gcgcacccgc aatatcttgg gcgggtcctc cgacaggtag
     1681 atctcccgct ccgcgggggc cccc
//
LOCUS       DASTJJ010000707         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_829723, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000707 DASTJJ010000000
VERSION     DASTJJ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829723"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFR02_08600"
     CDS             complement(<1..656)
                     /locus_tag="VFR02_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9870538.1"
                     /translation="MRAGVYWATFKASLQAKLEYKLDFAIGLLTSTMLQLAALGFLWV
                     VFHQASHLQGWDQAQVVFLFGLTATALGLSELFFNHIWMLPYYIVMGDLDRLMVYPVR
                     SLPFLLISRPELHSFGNLATGLVLCGASLGHLHRPWFDYALVPLWAACGSLIYTSALV
                     AFSSLSFRFVGPFSYSLMIPHALLQASRYPLNIYPGALHYGLLVLVPFGAFNYLPGGW
                     V"
     gene            complement(658..>1529)
                     /locus_tag="VFR02_08605"
     CDS             complement(658..>1529)
                     /locus_tag="VFR02_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870539.1"
                     /translation="DHRDAHLPPPGPGVKPLAHPLSPAQSARGYASLLFRALQANFAY
                     KGSTLIGLFTAALVYAITLLVWRHVYAQKGGSLALPPDQMFAYLALAFCLNYALTINI
                     DQRVGQRIRLGLIATDLLKPLDFQMTQAMQSLSDCLFNSLIGAAVFAAAYLFLGPSLL
                     PQSLPAFLLFLPSLALAFWIQYGIVFIFIQGAFYTYSGYGILTSRIALHQTFSGLQAP
                     LLLYPPLLRGLGDWLPFRHVIYTPILLYQGLAQGAEAWRLLAWQAFWALGLYLAGRWL
                     MRQALSQLEIQGG"
BASE COUNT          329 a          417 c          588 g          195 t
ORIGIN      
        1 acccatcccc ccggcaggta attgaaggcc ccgaagggca ccaacaccaa aaggccgtag
       61 tgcagggcgc ccggatagat gttgagggga tagcggctgg cctgcaggag cgcgtggggg
      121 atcatcaggc tgtagctgaa gggtccgacg aaccggaagg acaaactgga gaaggccacc
      181 agggccgagg tgtagatgag cgagccgcag gcggcccaga ggggcaccag ggcgtagtcg
      241 aaccagggcc ggtgcaggtg ccccagggag gccccgcaga ggaccaatcc ggtggccaag
      301 ttgccgaaag aatgcagctc gggccgcgag atgagcagga agggcaggga acggaccgga
      361 tagaccatca agcggtccag gtcgcccatc acgatgtaat agggaagcat ccagatgtgg
      421 ttgaagaaga gctcggagag gcccaaggcg gtggcggtca ggccaaagag gaagaccact
      481 tgggcctggt cccagccctg caggtgggag gcctggtgga agaccaccca gaggaagccc
      541 aacgccgcca actgcagcat ggtggaggtg agcaggccga tggcgaagtc cagcttgtat
      601 tccagcttcg cttgcaggga ggccttgaag gtggcccaat aaacgcccgc gcgcatgtca
      661 gcctccctgg atctcgagtt gggacagggc ctggcgcatc agccaccggc cggccaagta
      721 cagccccagc gcccaaaagg cctgccaggc cagcagccgc caagcctccg cgccctgggc
      781 caggccctgg tagaggagga tgggcgtgta gatgacgtgg cggaaaggca gccaatcgcc
      841 caggccccgc agcagcggcg ggtaaagcag caggggcgcc tgcagccccg agaaggtctg
      901 gtgcagggcg atgcgggagg tgaggatgcc gtaaccgctg taggtataga aggcgccctg
      961 gatgaagatg aagacgatgc cgtattggat ccaaaaggcc agcgccaggc tgggcaggaa
     1021 caacaggaag gccgggaggc tctggggcaa caggctgggc cccaggaaca ggtaggccgc
     1081 ggcgaagacc gccgccccga tgaggctgtt gaagaggcag tcgctgaggg actgcatggc
     1141 ctgggtcatc tggaagtcca ggggtttgag caggtcggtg gcgatgagcc ccaggcggat
     1201 gcgctggccc acccgctggt cgatgttgat ggtgagcgcg tagttcaggc agaaggccag
     1261 ggccaggtag gcgaacatct ggtccggggg gagcgccagg gagccgccct tttgggcgta
     1321 gacgtggcgc cagaccagca gcgtgatggc gtagaccagc gcggcggtga agaggccgat
     1381 gagcgtggac cccttgtagg cgaaattggc ctggagcgcc cggaacaaca gggaggcgta
     1441 gccgcgggcg ctttgggcgg ggcttaaggg atgggccagg ggcttcacgc cgggtccggg
     1501 gggcggtaga tgcgcgtcac gatggtctc
//
LOCUS       DASTJJ010000708         1845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1564858, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000708 DASTJJ010000000
VERSION     DASTJJ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564858"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1161)
                     /locus_tag="VFR02_08610"
     CDS             complement(187..1161)
                     /locus_tag="VFR02_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870540.1"
                     /translation="MIFCPACNAENPDGTKICVKCGTELPKAAPVAAPKPQGRPVAAA
                     PADPGKPYGVRDYGRDLGDALWLVAILALLLVGFLGEATHWSFKFTETDEAKLVQAPV
                     LEKPKPHHAMGRRRVSIPRHNPGMENAKPLVEFGNPDTLYAKGKRQFDDKNYHGSYES
                     LKKALEIDPTYAKAYFAMGYLYSRFNMNDVAVRMYEMALRFDPNFADPVNNLAMMYEK
                     AGNLDDALSLLQQAVTLNADNADCQYNLGSLYLDKDEPDQALDAFQKAAAIRPTDASI
                     YNDMALTYERLGKKQEAEDAWNKVLQYASTADYLKQAKTHLDYLQTQS"
     gene            complement(1292..>1845)
                     /locus_tag="VFR02_08615"
     CDS             complement(1292..>1845)
                     /locus_tag="VFR02_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9870541.1"
                     /translation="VIQQLSLASDQIGEFLATITSISRKTNLLALNAGIEAARAGEHG
                     QGFAVVASEIKTLAEASSRASGDVKHLVDDIRQRTASAITLINTTGKIEENVNLVYNA
                     GDVFMSIVKSIRKASGVLSEISQALEDQRNDNDLMLQLVSQVHQGGERARQQFDQVET
                     NLDRLKKNSRRVLEELERLGQAG"
BASE COUNT          267 a          589 c          609 g          380 t
ORIGIN      
        1 gcgcaaccgc agcgccaatt gccttttcaa ctcgaaaccg ccgtggtccg cggcgatccc
       61 cagccggcgc cccttcccgg gccccctctt cttggtcttg gtccgcatca cttttccttt
      121 ccatcgccgc tcaaaagcaa aaagcccccg ccggggaccc gggggggctt ttccgtccgg
      181 ccgtcctcag ctttgggtct gcaggtagtc caggtgggtc ttggcctgtt tcaggtaatc
      241 ggccgtggag gcgtactgca gcaccttgtt ccaggcgtcc tcggcttcct gcttcttgcc
      301 caagcgctca taggtcaggg ccatgtcgtt gtagatggag gcgtccgtcg ggcggatggc
      361 cgcggccttc tggaaggcgt ccagggcctg gtccggctcg tccttgtcca ggtacaggct
      421 gcccaggttg tactggcagt ccgcgttatc ggcgttgagg gtcaccgcct gctgcagcag
      481 ggacagggcg tcgtccaggt tgcccgcctt ctcgtacatc atggccaggt tgttcaccgg
      541 gtcggcgaaa ttcgggtcga accgcagggc catctcgtac atgcgcaccg ccacgtcgtt
      601 catgttgaac cgcgaataga ggtagcccat ggcgaaatag gccttggcgt aggtggggtc
      661 gatctccagg gccttcttca ggctctcata ggagccatgg tagttcttgt cgtcgaactg
      721 ccgcttgccc ttggcgtaga gcgtgtccgg gttgccgaac tccaccaggg gcttggcgtt
      781 ctccatgccc ggattgtgcc gcgggatgga gacgcgccgg cgccccatcg cgtggtgggg
      841 cttgggcttt tcgagcaccg gggcctgcac cagtttggcc tcatcggtct cggtgaactt
      901 gaaggaccaa tgggtggcct cgcccaggaa gccgaccaac aacaacgcca ggatggccac
      961 cagccacagg gcgtcgccca ggtcgcggcc gtaatcccga acgccgtagg gttttccggg
     1021 gtcggcgggg gccgccgcca cgggccgccc ttggggcttg ggcgcggcca ccggggccgc
     1081 cttgggcagc tcggtgccgc acttcacgca gattttcgtg ccgtccgggt tttcggcgtt
     1141 acaagccggg caaaagatca tggggaccct caggtcagga tgggacttca agcgagaaca
     1201 ggacaattta ccgcagggcg ccgaaacctg tcaacgtttc cagccgggcc cggaagcggg
     1261 cgtttgcacg tgtcacggac cggcgggcgc ctcaacccgc ctggcccagc ctctccagct
     1321 cttccagcac ccggcgcgaa ttcttcttga ggcggtccag gttggtctcc acctggtcga
     1381 actgttggcg ggcccgctcg cccccctggt ggacttggct gacgagctgc agcatcaggt
     1441 cgttgtcgtt gcgctggtct tccagggcct gggagatctc gctcaagacc ccgctggcct
     1501 tgcggatgga tttcacgatg gacatgaaca cgtcgcccgc gttgtagacc aggttgacgt
     1561 tctcctcgat cttgcccgtg gtgttgatga gcgtgatggc gctggccgtg cgctggcgga
     1621 tgtcgtccac caggtgcttc acgtcccccg aggcccggga ggaggcttcc gccaaggtct
     1681 tgatctcgct ggccaccacg gcgaaaccct ggccgtgttc cccggcccgg gcggcctcga
     1741 tgccggcgtt caaggccagc aggttggtct tgcggctgat ggaggtgatg gtggccagga
     1801 actcgccgat ctggtccgag gccaggctga gctgttggat gacct
//
LOCUS       DASTJJ010000709         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1320242, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000709 DASTJJ010000000
VERSION     DASTJJ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320242"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFR02_08620"
     CDS             <1..559
                     /locus_tag="VFR02_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870542.1"
                     /translation="ESRQVMDYLSVRYLVGKNRFAGLKPLGRAGVNPLFEDPTPLPKW
                     FSARRAVAAGDLAGDFREAEAPGWNYASDAFVADPARAGDYAPRRVREVARTPQRLEL
                     SASGRGRALLVSSETAYPGWKAWVEGRPRALETVNHVFRGLCLDDGETQASLVYAPDS
                     FRLGLFLGLLALGFWAGGWVRRSLA"
     gene            556..2676
                     /locus_tag="VFR02_08625"
     CDS             556..2676
                     /locus_tag="VFR02_08625"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET9870543.1"
                     /translation="MSDRKTAWMVGAAYLALLLVWAWRFTGHAPVMMDTLAYVFPEKW
                     VDVQAYRAGNIPLWNPWVACGLPHVGDLQSAAFYPPFWLWILTGLPDWFFWMALAHGL
                     LAALGFFLWARQGGAREPLAALTAVGFAGSALLVFYWGFPTHLATVAWVPWIFWATAR
                     FNARPSWMGWALLTLFWSLDFLAGYIYITFYAALLWAAWTWGILRPGLPKTAAYGASS
                     LAAWGLTACQWLPFLDFIGHSQRPGVLDAVYSLHAVNFLTLLSPDILGFPGTASYRGD
                     FPDYIFNNFYLGWVPLALWLAGWLWARGGADRFWRWAGLVVLLWTAGTHFPLWHLLPA
                     ALLGTLDPAKAAFLFVFCAFTALVFWIEPLLRRKSVGKKARRWITVLGVLWLLDLLLV
                     PFRVMHLMPDPYRDPQMARVASLVGQAVGQGRMVSLHRGDLPSAFPGAGPADSIRDHL
                     LELPPNSNLVFGIRSAAGYFSIFPDGYQDLQAYLNRGFPFEGRILDAAGVDALLTQDE
                     LSPFKYRLLGDWGPYRLSSNAGALPLLWLDYRVREFPDRPSVFGALLKPDAFLENELY
                     TEKSPDGHAVRLPPPGRSLPYGAPGLLDRWSGFWDRLGGSKLSTDARSPCRLGVEVNL
                     RDPGYVVWSQTFSPGWRAWVDGEPKPILRADGLFMAVVVPQPGLHRVDFRYEPTAFRL
                     GLFVSLLSVVLLGLVLPMGLLRRR"
BASE COUNT          339 a          873 c          947 g          658 t
ORIGIN      
        1 ggaaagccgc caagtgatgg attatctgtc ggtgcgctac ctggtgggga agaaccgttt
       61 cgcgggcctg aagccgctgg gccgggcggg ggtcaacccg ctttttgagg atccaacgcc
      121 gctgccgaaa tggttcagcg cgcggcgcgc cgtcgcggcc ggcgacctgg cgggggattt
      181 ccgggaggcc gaagcccccg gttggaatta cgcttccgac gctttcgtgg ccgatcccgc
      241 ccgggcgggg gactacgccc cccggcgggt gcgtgaggtg gcccgtacgc cccagcgcct
      301 ggaactttcg gcgtccggcc gggggcgggc cctgttggtc tcctcggaga cggcttatcc
      361 gggttggaag gcctgggtgg aggggcgccc ccgggccttg gagaccgtca accatgtctt
      421 ccgcggcctt tgtcttgatg atggcgagac ccaagcgtcc ctggtctacg cgcccgattc
      481 cttccgcctg gggttgttcc tcggcctgtt ggccctgggg ttttgggccg ggggttgggt
      541 ccggaggtcc ctggcatgag cgaccgtaag accgcttgga tggtgggcgc ggcctacttg
      601 gcgttgttgt tggtgtgggc ttggcgcttc acgggccacg cgccggtcat gatggatacc
      661 ctggcttatg tcttcccgga aaaatgggtg gatgtgcagg cctatcgggc cgggaacatc
      721 ccgctttgga acccctgggt cgcctgcggc ctgccccatg tcggggacct gcaatcggcc
      781 gccttttatc cgcccttttg gctttggatc ttgaccggac tgcccgactg gttcttctgg
      841 atggccctgg cccacggact actggcggcc ctgggcttct tcctttgggc ccggcagggc
      901 ggcgcccggg aacccctggc ggccttgacc gcggtgggtt tcgccggatc ggccctcctg
      961 gtcttttatt ggggtttccc cacccacctg gccacggtgg cctgggtccc ctggatcttt
     1021 tgggccacgg cccggttcaa cgcccgtcct tcttggatgg gttgggccct cctcacgctc
     1081 ttttggtcgt tggatttcct ggcgggttac atctacatca ccttttacgc ggcgctgctt
     1141 tgggcggctt ggacctgggg gatcctgagg cccggcctac cgaagaccgc ggcttatggc
     1201 gcttcgtcgc tggcggcctg ggggttgacc gcctgccagt ggctgccttt cttggatttc
     1261 atcggacatt cccagcggcc cggtgtcttg gatgccgttt acagcctgca cgcggtgaat
     1321 tttctcacgc tcctctcccc ggacatcctg ggttttcccg gcacggcgtc ctatcgaggg
     1381 gatttccccg actatatctt caataatttt tatctgggat gggtgccgct ggccctctgg
     1441 ttggcgggat ggctttgggc gcggggcggc gcggaccgtt tttggcgttg ggcggggttg
     1501 gtggtgttgc tttggaccgc cgggacccat tttccgcttt ggcatctgtt gcccgccgcc
     1561 cttttgggga ccctggaccc ggccaaggcg gcctttcttt tcgtcttctg cgcattcacg
     1621 gccctggttt tctggatcga gccgctcttg cgccggaagt ccgtggggaa gaaggcccgc
     1681 cgttggatca ccgtcctggg ggtgctttgg ctcctggacc tgctcctggt ccctttccgg
     1741 gtcatgcacc tgatgcccga tccctatcgg gatccccaga tggcccgggt ggcctccttg
     1801 gtggggcagg cggtggggca gggccggatg gtcagcctgc accgaggcga ccttccctcg
     1861 gctttcccgg gcgcgggccc ggcggattcc atccgggacc acctgttgga actgccaccc
     1921 aacagcaacc tggtgttcgg catccgttcc gcagcgggtt atttttccat cttccccgac
     1981 ggctaccagg acctgcaggc ctacctgaac cggggttttc cctttgaagg acggatcctg
     2041 gacgcggcgg gagtggacgc gttgttgacc caggacgagc tgtcgccctt caagtatcgg
     2101 ctcttgggtg attgggggcc ctatcgcttg agcagcaacg ccggggccct gccgctcctc
     2161 tggctggact atcgggtgcg ggaattcccg gaccgcccct cggtgttcgg ggccctttta
     2221 aaaccggacg ccttcctgga gaacgagctt tataccgaga agtcgcccga cgggcacgcc
     2281 gtgcgcttgc cgccgcccgg acgcagcctg ccttatgggg cgccgggttt gttggaccgg
     2341 tggtcgggtt tctgggatcg cttgggcgga tccaaattat ccaccgatgc ccggtccccc
     2401 tgccgcctgg gggtggaggt caacctgcgc gacccgggat acgtggtctg gtcccagacc
     2461 ttttcccccg gttggcgggc ctgggtggat ggggagccca aaccgatcct gcgcgccgac
     2521 gggcttttca tggccgtggt ggtgccccag cccgggctcc accgggtgga cttccgctat
     2581 gagccgactg ccttcagatt agggctcttt gtgagccttc tgtccgtggt cttgttgggt
     2641 ctggtccttc ctatgggtct cctgcgtcgc cgctaaagcc atgggatcct gttgagaagt
     2701 gttcggtcat cccaggcgct tcgaaggccg aaaaagggct ttttcttccg gagttcgaag
     2761 cctttcgggc ccgaaaatca tggtgaaaag ctttcgggta tataataatt gcgtcct
//
LOCUS       DASTJJ010000710         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_954784, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000710 DASTJJ010000000
VERSION     DASTJJ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954784"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..711)
                     /locus_tag="VFR02_08630"
     CDS             complement(118..711)
                     /locus_tag="VFR02_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870544.1"
                     /translation="MTATALGHHDTTPATSAAQDTVAAVQTDVTHSPVNTSAQAPSDQ
                     DLHPVAVSGDQKTFTPDAEQVANAKAIVDAAKDMGLSPRAYTIAVATSLQESNLHNLG
                     DLGAHNDHDSLGLFQQRTSTGGWGTAEQIQDPHHAATKFLERLVKVPGWDTMPLTQAA
                     QKVQVSAYPQAYAKHETQADNIITALYGTGPYANTTK"
BASE COUNT          146 a          328 c          427 g          192 t
ORIGIN      
        1 tcaaagcggt gcacgacccc gagaacgtgt ttcggatgaa caacaacatc acgcccgcag
       61 actgaatggc cgaaggcccg cctgccctgt ggtggggtgg cgggccttcg gggtgggtca
      121 cttggtggtg ttggcgtagg ggccggtgcc gtagagggcg gtgatgatgt tgtcggcctg
      181 ggtttcgtgc ttggcgtagg cctgtgggta ggcggagacc tggaccttct gggcggcctg
      241 ggtcagcggc atggtgtccc agccggggac cttgaccagt cgctcgagga acttggtggc
      301 ggcgtggtgc gggtcctgga tctgctcggc ggtgccccag ccgccggtgg aggtgcgctg
      361 ctggaacagg cccagcgagt cgtggtcgtt gtgggcgccg aggtcgccga ggttgtgcag
      421 gttggattcc tgcagggagg tggccacggc gatggtgtag gcccgtgggg acaggcccat
      481 gtccttggcg gcgtccacga tggccttggc gttggccacc tgctcggcgt cgggggtgaa
      541 cgtcttctga tcaccgctta ccgcgacggg gtgcaggtcc tgatccgagg gcgcctgcgc
      601 cgaggtgttg accgggctgt gcgtcacgtc ggtctggacg gcggcgacgg tgtcctgcgc
      661 cgccgacgtg gcgggggtgg tgtcgtggtg gcccagggcg gtggcggtca cgccgccgat
      721 accggccagg gtgaacgcgc caccggcgat gatgctctgc agacgggtca gcttacgcat
      781 cgtggaagtc ctctccaaga atgtcagcgg gatgcccgag gccacgcgca ccagcacggt
      841 ggctggcggg gccgtcacgc tccgtaaccc agggggttcg ggcgggggtc gggcatcgtt
      901 tcccggtcgt gccgggtgcg atgggtataa ccaccccgcc cccgaggcct atccccgacc
      961 gggggccggt ggccccggtc accgagtgtc accgcaccgt tgagccaacc cagcccgcac
     1021 acgtcccaga tccgggacga catcccaggc cgagaccgag atcatggcat gctatggccc
     1081 ctggcggggc cat
//
LOCUS       DASTJJ010000711         1187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_585847, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000711 DASTJJ010000000
VERSION     DASTJJ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585847"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFR02_08635"
     CDS             <1..1177
                     /locus_tag="VFR02_08635"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET9870545.1"
                     /translation="TSFLYAEAGAAPAAGPIVIGAARMDLYLPLLRGKRVGILTNQTA
                     LVDGVHLVDTLLKRKVDIVKIFGPEHGFRGTADAGATVSSGVDPVTGIPVISLYGKHN
                     TPNAADLEGVDVLIYDIQDVGTRFYTYISSMQRFMEAAARFGKPLIILDRPNPNGFYV
                     DGPVLEKKFSSGVGMQPIPVVYGMTIGEYARMLIGEHWLSDPSLHPELTVIPCLHYTH
                     DSLYTLLVKPSPNLPDMASVYLYPSLCFFEGTACSVGRGTAHPFQLFGHPSLPDSLYA
                     FTPEGMPGATHPKLQGKRCHGYLVATTAARALEEIRGALQLKWILTAYRLFPDKDHFF
                     TRYFNTLAGTDKLQEQIRAGWSEKRIRQSWQPALRKFKQIRKKYLLYPDFTGTAHQQ"
BASE COUNT          228 a          429 c          322 g          208 t
ORIGIN      
        1 cacctccttc ctctatgccg aagccggcgc agcccccgcc gccggcccca tcgtcatcgg
       61 agcggcgcgc atggacctgt accttcccct gctgcgcggc aagcgcgtag gcatcctcac
      121 caaccagacg gccctggtcg acggcgtgca cctggtggac accctgctga aaagaaaggt
      181 ggacatcgtc aagatattcg ggcccgagca cggcttccgc ggcaccgccg acgccggggc
      241 gacggtatcc agcggcgtgg acccggtgac cggcataccg gtcatctccc tgtacggcaa
      301 gcacaacacg cccaacgccg ccgacctgga gggcgtcgat gtgctgatct acgacatcca
      361 ggacgtcggg acccgcttct acacctatat cagctccatg cagcgcttca tggaagcggc
      421 cgcccgcttc ggcaagccgc tgatcatcct ggaccggccc aaccccaacg gcttttacgt
      481 cgacgggccg gtactggaaa aaaagttctc ttccggcgtg ggcatgcagc ccatcccggt
      541 ggtctacggc atgacgatcg gggaatatgc ccgcatgctc atcggggagc actggctctc
      601 cgaccccagc ctccaccctg agttgacggt gatcccctgc ctgcattaca cccatgacag
      661 cctctatacc ctgctggtga agccctcccc caacctgccc gacatggcct cggtctacct
      721 gtacccgtcc ctttgctttt tcgagggcac ggcctgcagc gtgggacgcg gcaccgccca
      781 tcctttccag ctcttcggtc atccctccct gcccgacagc ctgtacgcct tcaccccgga
      841 aggcatgccc ggcgccactc accccaagct ccagggcaag cgctgccacg gctacctggt
      901 ggccaccacg gctgcccgcg ccctggagga gatccgcggc gccctgcagc tgaaatggat
      961 cctcaccgcc taccggctgt ttcccgacaa ggatcatttt tttacccgtt atttcaatac
     1021 cctggccggc accgacaagc tgcaggagca gatcagggcc ggatggagcg agaaaaggat
     1081 ccggcaaagc tggcagcccg ccctgcgcaa gttcaaacag atccggaaaa aatacctgct
     1141 gtacccggat ttcacaggga cggctcatca gcaataaatt tatatgg
//
LOCUS       DASTJJ010000712         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1198011, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000712 DASTJJ010000000
VERSION     DASTJJ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198011"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFR02_08640"
     CDS             <1..890
                     /locus_tag="VFR02_08640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9870546.1"
                     /translation="RGSLAEDTRPGARSLLQKYEKKVRALEKESARAEKLWAYEREAA
                     RAGAKRPAGLDEAGRGPLVGAVVAAAVVLPEGWACAGLNESKKLTPEKRDKLFETITR
                     EALAFGVGEASAAEIDALNIYRATQAAMGRAVAALLAPPDFLLTDAMPLPEIRLPQKP
                     IVQGDALSASIAAASIVAKVTRDRQMAELDKRYPGYGFASHKGYGTEEHLKALDELGP
                     CPEHRVSFGPVAKAVVGRSTAGPAAYWQGRLEAAKNGEELRQVGHQIKRLGAADLKPA
                     ELGFLREAYRKKSASWGGE"
     gene            887..1285
                     /locus_tag="VFR02_08645"
     CDS             887..1285
                     /locus_tag="VFR02_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET9870547.1"
                     /translation="MTSSPRESHSEGRRGEARAAAYFRRRGWKVEAKNFRAGPGEIDL
                     IVSKEGQLVFVEVKMRRGSATGDPLEAVSPRKVQRVSAAAAAYLSQRGGDTPHCRFDI
                     LTLGPDKTLFGTLKMRHYENAYESDGRYMV"
     gene            1365..>1733
                     /locus_tag="VFR02_08650"
     CDS             1365..>1733
                     /locus_tag="VFR02_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polya polymerase"
                     /protein_id="HET9870548.1"
                     /translation="MKTGLPSKIPPKTLALLKKLGALADSRGEAAYAVGGFVRDLFLK
                     VPGVDIDVTVVGDGVTFAEKFAELTGSEVEAFTRFGTSIVVVPGFGKVDIATARTESY
                     ERPGALPLVQKGGIVQDLYRR"
BASE COUNT          290 a          578 c          608 g          257 t
ORIGIN      
        1 tccggggctc cctggcggaa gacacgaggc cgggcgcccg cagcctcctc caaaaatatg
       61 aaaagaaggt ccgggccttg gaaaaggaat cggcccgggc cgagaagctg tgggcctacg
      121 agcgggaagc cgcccgcgcc ggggccaagc gccccgccgg gttggacgag gcgggccggg
      181 ggcccctggt gggggccgtg gtggccgcgg cggtggtgct gccggagggc tgggcctgcg
      241 cggggctcaa cgagtccaag aagctcaccc ccgagaaacg ggacaagctc ttcgagacca
      301 tcacccggga agcgctggcc ttcggggtgg gggaagcttc ggccgccgag atcgacgccc
      361 tcaacatcta ccgggccacc caggccgcca tgggccgcgc ggtggcggcc ctcttggccc
      421 ccccggactt cctcttgacc gacgccatgc ccctgccgga gatccgcctt ccccaaaaac
      481 ccatcgtgca gggcgacgcc ctttcggcca gcattgcggc ggcctcgatc gtcgccaagg
      541 tcacccggga ccgccaaatg gccgagctgg acaagcgtta tcccggctat ggtttcgctt
      601 cccacaaggg ctacggcacc gaggaacacc tgaaggcgct cgacgaactg ggcccctgcc
      661 ccgagcaccg ggtctccttc gggccggtgg ccaaggccgt ggtgggccgc tcgaccgcgg
      721 gcccggccgc ctactggcaa gggcggctgg aggcggccaa gaacggcgaa gaactgcgcc
      781 aggtcggcca ccagatcaag cgcctgggcg cggcggacct gaagcccgcc gaactgggat
      841 ttttgcggga ggcctatcgg aaaaagtcgg cgtcctgggg cggggaatga cttccagccc
      901 ccgggaaagc cactcggagg ggcgccgggg agaagcgcgg gcggcggctt atttccgccg
      961 gcgcggttgg aaggtggaag ccaagaattt ccgggccggg cccggcgaga tcgacctcat
     1021 cgtctccaaa gagggccagt tggtcttcgt ggaggtcaag atgcggcggg gcagcgccac
     1081 cggcgacccc ctggaggccg tgtcgccccg caaggtgcag cgggtctcgg ccgccgccgc
     1141 ggcctacttg tcccaaaggg gcggggacac gccgcactgc cgcttcgaca tcctcacctt
     1201 aggccccgac aagaccctct tcggcacgct caaaatgcgg cattatgaga acgcctatga
     1261 gtcggatgga aggtatatgg tctgatccgg gactgggccg cctgagatcc cgatcggccc
     1321 cggtaagtct tggtgccctt cctttggcct tctataatgt ccccatgaag acaggcctgc
     1381 cctccaagat cccccccaag accctggcgc tgctcaagaa gctcggcgcg ctggcggatt
     1441 cccgtgggga agcggcttac gccgtgggcg ggttcgtgcg ggacctcttc ctcaaggtgc
     1501 cgggcgtgga catcgacgtg acggtggtgg gggacggcgt caccttcgcg gaaaaattcg
     1561 ccgagctcac cggttccgag gtggaggcct tcacgcgttt cggcacctcc atcgtggtgg
     1621 tgcccggttt cggcaaggtg gacatcgcca ccgcccgcac ggagagctac gagcggcccg
     1681 gcgccctgcc tttggtccaa aagggcggca tcgtgcagga cctctaccgg cgg
//
LOCUS       DASTJJ010000713         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_221619, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000713 DASTJJ010000000
VERSION     DASTJJ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221619"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..945
                     /locus_tag="VFR02_08655"
     CDS             409..945
                     /locus_tag="VFR02_08655"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9870549.1"
                     /translation="MVRVWMRQLTVWDTKVFHLFFGKVDRKSIRRFFLLVSRSADPLS
                     CALWGLAGGIWLPGAAPFLAAGLAAFGLELSLYYLLKKGIRRPRPFQTLAGVRFLIAP
                     PDEFSFPSGHTAAAFLMATLVTAACPWLAGPAFLWAGMVGFSRVYLGVHYPTDVLAGM
                     ALGFFSAQAGWVAVHSWM"
     gene            1230..>1824
                     /locus_tag="VFR02_08660"
     CDS             1230..>1824
                     /locus_tag="VFR02_08660"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HET9870550.1"
                     /translation="MKRFWLFGLFLAGALVALNAPVVAKDAMADPQSMDDMSNQMDDI
                     NSALGSMAVKMNEMDAKMGLKFSGDMRIRYAFISQNSSQQGVTIADQSMGRYRARLGA
                     EITRGDFTGKVRFATGSTNSPWSQNNTFDTAMVDPTINVDTAMITWKPSFASGMLTVA
                     AGKMGNPLTKTAITWDPDIQPEGALVEFKKGDFTLRGT"
BASE COUNT          337 a          528 c          583 g          376 t
ORIGIN      
        1 aggcggaccg gttcccgcag gtcctccacc acatggaggt tgcggtgacg gtcgacaaaa
       61 ccgatggcca agcgcatggc gttggatccg acgtcgatgg agcctaaggt cggcatgaag
      121 gtcctttggg tgaaaaggga acaattcccg tccggagcgc cccgggggac cccgttatat
      181 aagaggatct tcccgatccc cgtgttaatt cgttgttaag cctgccgccc gcgccttctt
      241 tcaacggggc tttcccagga gtttagccgg aaatcggctt aaaacaaggg cttgggcggg
      301 ggcgggcctg tggataagtt tcggcccggt gacgcgttat ttacgatccg gaaaccggcc
      361 ctccttaaac ttttcccatc aaaagtccgt ttccggggaa ggcaaggcat ggtacgggtc
      421 tggatgaggc aattgacggt ttgggacacc aaggtgttcc atcttttttt cggcaaggtg
      481 gaccggaagt ccatccggcg atttttcctg ttggtgtccc ggagcgccga tccgctctcc
      541 tgcgcccttt ggggcctggc ggggggcatt tggctgcccg gtgccgcccc tttcctggcg
      601 gcgggcctgg ccgccttcgg cctggagctt tccttatatt acctcctgaa aaaagggatc
      661 cggcggcccc gtccgttcca aaccttggcg ggcgtccggt tcttgatcgc gccgcccgat
      721 gaattcagtt ttccctcggg gcataccgcc gcggctttct tgatggccac cttggtgacg
      781 gcggcctgcc cctggttggc cgggcccgcc tttttgtggg ccgggatggt gggcttttcc
      841 agggtctacc tgggggtcca ttaccccacg gacgtgctgg cgggaatggc cctgggattc
      901 ttcagcgccc aagccggatg ggtggccgtc cattcctgga tgtaggcacc cccacccctg
      961 gagggggacc ttcggggcgg ttcaagaatc gcctttggaa aacgaaaata ttttttgttc
     1021 cggcggccga cggcggggaa aaaccacaag atttagtggg gcggcggcgc aaaccggtca
     1081 gagtttacac gcatttaact tcatggcaac aaacgggccg ggcgggctgc ctatagttcg
     1141 cccagctttg cttccttgcc ggcggccgat gggtcaaagg cttttcgggg ccggagatga
     1201 agcaaagtaa caaaaacaaa ggagcgagta tgaaacggtt ttggttgttc ggactgtttt
     1261 tggcgggcgc gctggtggcc ctgaatgcgc cggtcgtcgc caaggacgcg atggcggatc
     1321 cgcagtcgat ggatgatatg agcaatcaga tggacgacat caacagcgcc ttgggctcca
     1381 tggcggtgaa gatgaacgag atggacgcca agatgggcct gaagttctcc ggggacatgc
     1441 gtatccgtta cgctttcatc tcccagaact cgagccagca gggtgtgacc atcgcggacc
     1501 agagcatggg ccgctaccgg gcccgcctgg gcgcggagat cacccgcggg gacttcacgg
     1561 gcaaggtccg tttcgcgacg ggttcgacca actccccctg gagccagaac aacaccttcg
     1621 acaccgccat ggtggacccc acgatcaatg tcgacacggc gatgatcacc tggaagcctt
     1681 ctttcgccag cggcatgctg acggtcgccg cggggaagat gggcaacccc ctcaccaaga
     1741 cggccatcac ctgggacccg gacatccagc cggaaggcgc cttggtcgag ttcaagaagg
     1801 gtgacttcac cctgcggggc acct
//
LOCUS       DASTJJ010000714         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_221737, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000714 DASTJJ010000000
VERSION     DASTJJ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221737"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFR02_08665"
     CDS             <1..593
                     /locus_tag="VFR02_08665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9870551.1"
                     /translation="ADRDHFALKGERRECTLLYAELKGFNPLSENLAPDRLVEVLNQY
                     FSLAHGAVFQQEGMLDKFMGESFLAVWGAPFIHEDKEARAVKAAQEIHASLKELNLER
                     VKKGQAAFQWGIGVFTGQVVTGNLGSNRRNDYTVIGPAAETARKLCAKAAPGQTLVDR
                     GTYDKVESLVRVQELEAMVLPGVPEPLQVYEVVELI"
     gene            957..>1336
                     /locus_tag="VFR02_08670"
     CDS             957..>1336
                     /locus_tag="VFR02_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9870552.1"
                     /translation="MIETPRSIAQPLLKWYDRAKRDLPWRGIQNPYATFVSEMMLQQT
                     QVKTVLPYYGRFLKAFPDWKALARAKPDQVLKLWEGLGYYRRARNLQAAAQKVMADFK
                     GRLPDNLEDILTLPGVGRYSAGAVL"
BASE COUNT          283 a          412 c          395 g          246 t
ORIGIN      
        1 tggcggaccg ggaccatttc gccctgaagg gcgaacgccg ggaatgcacc ctgctttacg
       61 ccgaattgaa gggtttcaac cccttgagcg agaacctggc ccccgaccgg ctggtggagg
      121 tgctcaacca gtatttctcc ctggcccacg gcgcggtttt ccagcaggaa ggcatgctgg
      181 acaagttcat gggcgagtcc ttcctggcgg tgtggggcgc ccccttcatc cacgaggaca
      241 aggaagcccg ggcggtcaag gccgcccagg agatccacgc ctcgctcaag gagttgaacc
      301 tggagcgggt caagaaaggc caagccgcct tccaatgggg catcggcgtc ttcacgggcc
      361 aggtggtgac cggcaacctg gggtccaacc ggcgcaacga ctatacggtc atcggccccg
      421 cggcggagac cgcgcggaag ctctgcgcca aggcggcccc gggccagacc ctggtggacc
      481 ggggcaccta cgacaaggtg gagagcctgg tccgcgtcca ggaactggag gccatggtgc
      541 tgcccggcgt ccccgaaccc ctgcaggtct acgaggtcgt ggaactgatc tgaacccgaa
      601 aggaaccgaa gccaaccgcc aacccgacgg aaaaattttc accaccaaga caccaagtca
      661 ccgagaaaat caattgaagc gatccaggat tttccggatt ttttcaactc tcctgctttg
      721 gcagcccttg gtgccttggt ggcgaaacag gtttttgctt cttagaagtc atttcaaaac
      781 ctcttttacc gcagagacgc agaggcgcaa agtgaagcca aggggcgaaa aaccggggaa
      841 acaaacatta tttttcctgc gtctccgcgt ctctgcggtt ggaaattttc gcttttaggg
      901 ttttgaaaca ggttcttagt tttgtggttc taattttcaa tccggccccg gacaaaatga
      961 tcgaaacccc cagatccatc gcccaacccc tgctgaaatg gtacgaccgc gccaagcggg
     1021 acctgccctg gcggggcatc caaaacccct acgccacctt cgtgtccgag atgatgctgc
     1081 aacagaccca ggtcaagacc gtccttccct attacggccg tttcttgaag gccttccccg
     1141 actggaaggc cctggcccgg gccaagcccg accaggtgct caagctctgg gaagggctgg
     1201 gctattaccg ccgggcgcgg aacctgcagg ccgccgccca aaaggtgatg gcggatttca
     1261 aggggcggct tcccgataac ttagaggaca tcctgaccct gccgggcgtg ggccgctaca
     1321 gcgccggggc ggtcct
//
LOCUS       DASTJJ010000715         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_98030, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000715 DASTJJ010000000
VERSION     DASTJJ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98030"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1214)
                     /locus_tag="VFR02_08675"
     CDS             complement(<1..>1214)
                     /locus_tag="VFR02_08675"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9870553.1"
                     /translation="SLFLAIASLLGVSLVSYTTMRHIDDKTDWVEHSYEILQLVQKLR
                     SDVRNADNPLQSYLLTNNSQYKRVYDAEKTLILLDDRQLAALTSDNPIQAHYLDVLHP
                     LLDSEMGSMEEAARLAEAHRTQELLRFLQLQGAQNLERKIESCLSNMEIAERQLLQRR
                     VTEVDNGIRAAYLLICGGGILTALFCAAAAFFIRRQMRQRDQAAQLLASSEERFRSLA
                     ESAVDAFITIDSAGVIVDANPAGEALFGYDAGEMEGKPLAETFPRRFLQDHGDGALEA
                     FLKMTDSSQGTRSVELFGRRKDRSEFPVEVSVAAWSTRDGDFRTVFARDITERKFFTK
                     MLLKNEHRLFQFLEAIPVGIFVTDHTGKPYYANQAAKTLLGKPLDPRTSLEKLPEFYR
                     LHQAGGALYPPD"
BASE COUNT          193 a          339 c          457 g          225 t
ORIGIN      
        1 gtccggggga tagagggcgc cgccggcctg gtggagcctg tagaactcgg gcagtttttc
       61 cagggacgtg cgggggtcca ggggtttgcc caagagcgtt ttggcggcct ggttggcgta
      121 gtagggtttg cccgtgtggt cggtgacgaa gatgcccacc gggatggctt ccaggaactg
      181 gaagaggcgg tgctcgttct tgagcagcat cttggtgaag aacttgcgct cggtgatgtc
      241 gcgggcgaag accgtgcgga agtcgccgtc ccgggtgctc caggcggcca ccgagacctc
      301 cacggggaac tcgctccggt ccttccggcg gccgaacagt tccacggacc gggtgccctg
      361 ggagctgtcg gtcatcttca ggaaggcctc cagggcgccg tccccatggt cctgaaggaa
      421 acgccggggg aaggtctcgg cgaggggttt gccttccatc tccccggcgt cgtagccgaa
      481 gagcgcttcg ccggccgggt tggcgtccac gatgaccccc gccgagtcga tggtgatgaa
      541 ggcgtccacg gccgactccg ccagggagcg gaagcgttcc tcgctggagg cgagcagttg
      601 ggcggcctgg tcccgctggc gcatctgccg gcggatgaag aaggccgccg ccgcgcagaa
      661 gagggcggtc aggatcccgc cgccgcagat cagcaggtag gcggcccgga tgccgttgtc
      721 cacctcggtg acgcgccgct ggagcaactg acgctcggcg atctccatgt tggaaaggca
      781 ggattcgatc ttgcgctcca ggttctgggc gccctgcagt tggaggaacc ggagcagttc
      841 ctgggtgcgg tgggcctcgg ccaggcgggc ggcttcttcc atcgacccca tttcggaatc
      901 caagagggga tgcaggacgt ccaggtaatg ggcctggatg gggttgtcgg aggtcagggc
      961 cgcgagctgc cggtcatcca gcaggatgag ggtcttttcg gcgtcgtaga cgcgcttgta
     1021 ctgggagttg ttggtgagca ggtagctctg gagcgggttg tcggcgttgc ggacgtccga
     1081 gcgcagtttc tgcaccaatt ggaggatctc ataggagtgc tccacccaat cggtcttgtc
     1141 gtcgatgtgc cgcatggtgg tataggagac caggctgacg cccaagagcg aggcgatggc
     1201 caggaagagg ctga
//
LOCUS       DASTJJ010000716         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_709230, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000716 DASTJJ010000000
VERSION     DASTJJ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709230"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..2127)
                     /locus_tag="VFR02_08680"
     CDS             complement(223..2127)
                     /locus_tag="VFR02_08680"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET9870554.1"
                     /translation="MTKKPRPKRPLRRPPPRPPQGEPGAAAPAAPAPWHLSENLLLTF
                     FFAVMLVLPLIDILGRKLHQFAAFFPANPVPGSLNLVTHSTLAVMMVGGMIAAREGKL
                     LALSTTASLIPARFKDAARVFTAGLGAALTFYLVKASVSYMLIEYHVGSIIACGIPKW
                     AFLALLPLGFTAILARLVWRASGKNSQRLATLVLAGLLALLGPVVHQPSETFIYVCFG
                     VLMVSVVLGAPVFLALGGAALIFLWNSPTPIDSIPAETYRMVVSPTIPTIPLFTLTGY
                     FLAEGGASHRLLKVFQALFGWFRGGSAIVTALVCAFFTCFTGGSGVTILALGGLLMPI
                     LLAERYKQREALGLLTSAGSLGMLFPPSLPVILYGVVSHTPIDKLFLGGLLPGFILMV
                     ITAWWGVSKGSKAQTARHAFHLEGALAAVWEAKWELALPLVVFLFLFIVKVTLIESAA
                     LTALYAFVVEAFIYRDLPFSRIPKVVTECGILVGGVLLILGVSLGFTNDMVDAQVPDQ
                     LAAWVQGAVHSQWLFLLMVNLMLIIVGSLLEIFAAIVVVVPLLIPMANAFGVDPVHMG
                     IVFLLNMELGFLAPPLGLNLFLSSYRFKKPMTEVYWAVLPMYLILHAMVLAITYIPFL
                     TTYLPRHLSW"
     gene            complement(2124..3149)
                     /gene="dctP"
                     /locus_tag="VFR02_08685"
     CDS             complement(2124..3149)
                     /gene="dctP"
                     /locus_tag="VFR02_08685"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET9870555.1"
                     /translation="MKRPLTLILALAALLATAGLQAQEAAVTVKLGTLAPLGSPWDKI
                     IRDMGEKWRASGVLLKVYPGGELGDEPDMVSKMRTGQIQAGVFTVVGLSQIDKGFSAL
                     SVPMLYKSDAESDYVRSKLQPILEKRLLADGFKILNWGDAGWVMFFGRDPILTPDDLK
                     KAKFFIWGNDTDTMNIWKSAGYSPVPLTATDILPNLQTGLINSFDTTPLSALSFQWFP
                     LAPNMTNLKWAPLVGATIITTKAWNAIPAASRPAVLTAAAEAGKRFKDEIRADNQAAI
                     DAMKERGLVVHDITPEQYAQWEKLFESVYPQISGTVVPADMMSLAVKYRDQYRASHKD
                     GAPAPGE"
     gene            complement(3202..>3542)
                     /locus_tag="VFR02_08690"
     CDS             complement(3202..>3542)
                     /locus_tag="VFR02_08690"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET9870556.1"
                     /translation="YALEPDYDDGAIESFLITYEGGKPAAMGGSEAKARAHFKRAVEL
                     SHGTQLGPYLNLAEAVDVQTQDRDEFVSLLDQALAIDVEKDKPHRLVNLIMRKRAQWL
                     LGQVDSLFVK"
BASE COUNT          680 a         1026 c         1285 g          551 t
ORIGIN      
        1 aaacggcctt gctcatttgg ccgagggcgt cgacctggtc gggcctgagt tgttccagcg
       61 gcatgggcgg caccttcttg gcgtggaaaa gtgtggtcaa aagatccgat gaaaaaatag
      121 tagcacattt ggcgccaagg ccaaggggaa gggaacatcg gccgccggaa aaagatccga
      181 aatttggttt gaagcgttaa accggttggc ggggaccgcc gcctaccagc tcaagtgccg
      241 gggcaggtag gtggtgagga aggggatgta ggtgatggcc agcaccatgg cgtggaggat
      301 caggtacatg ggcagcacgg cccagtagac ctcggtcatg ggcttcttga agcggtagga
      361 cgacaggaac aggttgaggc cgagcggcgg ggccaggaag cccagctcca tgttcaacaa
      421 aaagacgatg cccatgtgca ccgggtccac gccgaaggcg ttggccatgg ggatgagcaa
      481 cggcaccacc accacgatgg cggcgaagat ctccagcagg ctgccgacga tgatgagcat
      541 gaggttgacc atcaggagga acaaccactg ggaatgcacg gccccttgca cccaggcggc
      601 caattggtcg gggacctggg cgtccaccat gtcgttggtg aagcccaacg acacgcccaa
      661 aatgagcagc acgcccccca ccaggatgcc gcattcggtc accaccttgg ggatgcgcga
      721 gaagggcagg tcccggtaga tgaaggcctc caccacgaag gcgtagaggg cggtgagcgc
      781 ggccgattcg atcagggtca ccttcacgat gaagaggaac aggaacacca ccaggggcag
      841 ggccagctcc cacttggcct cccagaccgc ggcgagggcc ccctccaggt ggaaggcgtg
      901 ccgggcggtc tgggccttcg aacccttgct cacgccccac caggccgtga tgaccatcag
      961 gatgaacccg ggcaaaagcc cccccaggaa cagtttgtcg atgggcgtgt gcgacaccac
     1021 gccgtacagg atgacgggca ggctgggggg gaagagcatg ccgagcgacc ccgcggaggt
     1081 gagcagcccc agggcttccc gctgcttgta tcgttccgcc agcaggatgg gcatgagcag
     1141 cccgcccaag gccaggatgg tcacgcccga cccgccggtg aagcaggtga agaaggcgca
     1201 gaccagcgcg gtcacgatgg cggacccgcc ccggaaccac ccgaagagcg cctggaaaac
     1261 cttcaagagg cggtgggacg cgccgccttc ggccaagaag taaccggtca gggtgaagag
     1321 cgggatggtg gggatggtgg gcgagaccac catgcggtag gtctccgccg ggatggaatc
     1381 gatgggtgtg ggagagttcc agaggaagat gagcgccgcc ccgcccaggg ccaagaagac
     1441 cggcgcgccc aggaccaccg acaccatcag cacgccgaaa caaacgtaga taaaggtctc
     1501 ggagggctgg tggaccaccg ggcccagcag ggccaaaagg ccggccagga ccagtgtggc
     1561 cagccgctgg gaattttttc ccgaggcgcg ccagaccagc cgggccagga tggcggtgaa
     1621 gcccagcggc agcagggcca ggaaggccca cttggggatt ccgcaggcga tgatggagcc
     1681 cacgtggtat tcgatgagca tgtaggagac gctggccttg accaggtaga aggtgagcgc
     1741 ggcgcccagg ccggccgtga agacccgggc ggcgtccttg aaacgagccg ggatgagcga
     1801 ggccgtggtg gaaagggcca acaacttgcc ctcgcgggcg gcgatcatgc cgcccaccat
     1861 catgacggcc agggtgctgt gggtgaccag gttgagcgat cccggcaccg ggttggcggg
     1921 gaagaacgcg gcgaactggt gcagcttgcg gcccaggatg tcgatgaggg gaagcaccag
     1981 catgacggcg aagaaaaagg tcagcaggag gttctccgaa aggtgccagg gcgcgggcgc
     2041 ggccggcgcg gcggcgcccg gttccccttg ggggggccgc ggcggcggac ggcggagggg
     2101 tcttttgggg cgcggctttt tcgtcattcg cccggggccg gggcgccgtc cttgtgcgag
     2161 gcgcggtatt ggtcgcggta cttgacggcc agggacatca tgtccgcggg caccacggtg
     2221 cccgagatct ggggatagac gctctcaaag agcttttccc actgggcata ttgctcgggg
     2281 gtgatgtcgt gcaccaccag gccccgctcc ttcatggcgt cgatggcggc ctggttgtcg
     2341 gcgcggatct cgtccttgaa gcgcttgccc gcctcggcgg cggcggtcaa gaccgccgga
     2401 cgggaggctg cggggatcgc gttccaggcc ttggtggtga tgatggtggc gcccaccagg
     2461 ggggcccact tcaggttggt catgttgggg gccaggggga accactggaa ggaaagggcc
     2521 gacaagggcg tggtgtcgaa ggaattgatg agcccggtct gcaggttggg caggatgtcg
     2581 gtggcggtca ggggcacggg cgagtacccg gcggacttcc agatgttcat ggtatccgtg
     2641 tcgttgcccc agatgaagaa cttggccttt ttcaggtcgt cgggggtgag gatggggtcc
     2701 ctgccgaaga acatgaccca acccgcgtcg ccccagttga ggatcttgaa gccgtcggcc
     2761 agcaggcgct tttcgaggat gggctgcagt ttggaacgca cgtagtcgct ttcggcgtcc
     2821 gacttgtaga gcatgggcac cgacagggcg gagaagccct tgtcgatctg ggaaagcccc
     2881 accacggtga agacgcccgc ctggatctgg cccgtgcgca tcttgctgac catgtcgggt
     2941 tcgtcgccca gttcaccgcc cgggtagacc ttcaacagca cgcccgaggc gcgccacttc
     3001 tcgcccatgt cgcggatgat cttgtcccag ggcgagccca ggggggccag ggtgcccagt
     3061 ttgaccgtga cggcggcttc ctgggcttgg agcccggcgg tcgccagcag ggcggccagg
     3121 gccaggatga gggtcaaggg tcgtttcacg ggaagcctcc taaaaaagtt cacgggcgga
     3181 aggcgccgcg gcgccccggc cttatttcac gaaaagcgaa tccacctggc ccagcagcca
     3241 ttgggcgcgt ttgcgcatga tcaggttgac caaacggtgg ggtttgtcct tttccacgtc
     3301 gatggccagg gcttggtcga gcagcgacac gaactcgtcc cggtcctggg tctgcacgtc
     3361 caccgcctcg gccaggttga ggtaggggcc caactgggtg ccgtgggaaa gttccaccgc
     3421 gcgcttgaag tgggcccggg ccttggcctc ggacccgccc atggcggcgg gtttgccccc
     3481 ctcgtaggtg atgaggaagc tctcgatggc gccgtcgtcg taatcgggtt ccagcgcgta
     3541 gg
//
LOCUS       DASTJJ010000717         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1443701, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000717 DASTJJ010000000
VERSION     DASTJJ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443701"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..>1017)
                     /locus_tag="VFR02_08695"
     CDS             complement(186..>1017)
                     /locus_tag="VFR02_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9870557.1"
                     /translation="ILSKGSSILLITAVAVILSQLVALGEKAVLAEYDVKAADNLGAR
                     KIYTQVHVISKTLYVLITLFAIASILMLFKEVQRFGASILASAGVVGIVLGFAVQKTI
                     ANIFAGFQLALTQPIRLDDVVIVEGEWGRIEEITLTYVVVHIWDDRRLVVPLSYFIEK
                     PFQNWTRTSAGLLGSVFVWADYTLSVEEVRRALKEIIEPHPLWDKRFWNLQVTDATEH
                     TQQLRVLATSEDSSKSWDLRCDIREKLIARIQRDFPQCLPRFRADLFPAPGDKGIPPR
                     "
BASE COUNT          197 a          311 c          322 g          187 t
ORIGIN      
        1 cgatggccag caatatcacc acgaccgcca tgctcttttt gaggttctcc cgttttttca
       61 tgctttcacc ttaaagagga tataaaccgc ccgccaattt gaacgattat acccgacttt
      121 ttgaaaatga gaacgtaaag aacccgttag gccccaaaac cgggccattg ggcctctccc
      181 ggggcttaac ggggcgggat ccccttgtcc ccgggggcgg gaaaaaggtc ggcccggaag
      241 cgcggcaggc actgggggaa gtcccgctgg atgcgggcga tgagcttttc ccggatgtca
      301 cagcgcaggt cccaggactt ggaggaatct tccgaggtcg ccagcacgcg cagctgctgg
      361 gtgtgctcgg tggcgtcggt cacctgcagg ttccagaagc gcttgtccca gagggggtgg
      421 ggctcgatga tctccttcaa ggcccggcgc acctcctcca ccgacagggt gtagtcggcc
      481 cagacgaaga ccgaccccaa gagcccggcg gaggtgcggg tccagttctg gaagggcttc
      541 tcgatgaagt agctcaaggg caccaccagc cgccggtcgt cccagatgtg caccaccacg
      601 taggtgaggg tgatctcctc gatgcggccc cactcgccct ccacgatgac cacgtcgtcc
      661 aggcggatgg gctgggtcag ggccagttgg aaccccgcga agatgttggc gatggtcttc
      721 tgcacggcga agcccagtac gatgcccacc acccccgccg aggccaggat gctggcgccg
      781 aaacgctgca cttccttaaa gagcatgagg atggaggcga tggcgaagag cgtgatgagc
      841 acgtagaggg tcttgctgat gacgtgcacc tgggtgtaga tcttgcgggc ccccaggttg
      901 tccgccgcct tgacgtcgta ctcggccagc acggcctttt cgcccaacgc caccaactgg
      961 ctcaggatga ccgccaccgc cgtgatgagc aggatgctgc tgcccttgga caggatg
//
LOCUS       DASTJJ010000718         1792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_956053, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000718 DASTJJ010000000
VERSION     DASTJJ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956053"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..560
                     /locus_tag="VFR02_08700"
     CDS             285..560
                     /locus_tag="VFR02_08700"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9870558.1"
                     /translation="MTKSELLGSLANDTGLKKKDVDNVMQALRNTIYKTLKTEYKIKL
                     DGLGVFQLKDRKARQARNPRTGEMVNVPAKKVVKFRVLKDLKEAVLS"
     gene            649..>1792
                     /locus_tag="VFR02_08705"
     CDS             649..>1792
                     /locus_tag="VFR02_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870559.1"
                     /translation="MAKSARRSLGVPGLPALLTAGLCAWTLFVFIRYFDPGALPGQAA
                     AFFASWAPGRFSEAGWPQHLGVWAESLGLLAVSLLALLNNWWLGAVLLERLGLSRVKS
                     PVRWGLESALGALASSLFWLGLGFSKLWEPAPLIFLGLVAAGPAGWKLRERLRASRPF
                     PAPPGPLLGALTAAALAAWALGFLQAFLPETFYDSLNYFLGVPHFWALRHGVCDEPGQ
                     ILSGYFHGGTLFLMDGFYFGGAGAAKMLAFFSAFACAAFAGAWAGEEAGVGAGWTAFG
                     LTAAFPLLALNGWAARVDGLLTFCLLAFFYACRKCFPAAAPGWGAAAGLLGGYAVTVK
                     PTALVGIAAVLLCLLPAWRRAPGRAWLTPLALGAGVVAPWLAKNACF"
BASE COUNT          268 a          576 c          571 g          377 t
ORIGIN      
        1 cgaatccctc agtgaaaggg ccttcctaca agagctttcg ctcttccggg cccggcggga
       61 attctcgaag accttcatcc agtacctgct caaccagccc aatatccaac gggaccggct
      121 ggcgtccttg atgaatgtga gcctgcggac cctgcaccgc tacctttcgg cctgaccagg
      181 cccccaaaag gccaaaaaat aagggaaaaa ggccaaaaaa gccttgccaa gaccctggcc
      241 tcttgccaca ataatcctcg acgcatcaat tcaggaggtg gacgttgacc aagagcgaac
      301 tgctgggaag cttagccaat gacacgggtt tgaagaaaaa agacgtggat aacgtgatgc
      361 aggcccttcg caacacgatc tacaagaccc tgaagaccga atacaagatc aagctggacg
      421 gcttgggcgt gttccagttg aaagaccgca aggcccgcca ggcccgcaac ccccgcacgg
      481 gcgagatggt gaacgtgccc gccaagaagg tcgtcaagtt ccgcgtcttg aaagacctga
      541 aggaagccgt cctgtcctaa tccgggcttt ttgagtcttt tccgagcacc ctgttgaaaa
      601 gcggggtgct tttttatttc ccgcccgccc gcaaggagaa ctaggccctt ggcaaagtcc
      661 gcgcgccgat cgttgggggt cccaggcctg cccgcccttt taacggcggg gctttgcgcc
      721 tggacgcttt tcgtttttat ccgctatttc gatcccggcg ccctgccggg acaagcggcc
      781 gcttttttcg cctcctgggc ccccggacgt ttctcggaag cgggatggcc acaacatttg
      841 ggggtctggg ccgaaagcct gggccttttg gccgtttcct tgttggcgct cctcaacaac
      901 tggtggttgg gcgcggtcct attggaaagg ctgggcctgt cccgggtgaa gtccccggtc
      961 cgctggggct tggagtcggc cctgggggcc ttggcttcca gtttgttctg gttgggtctg
     1021 gggttttcga aactatggga gcccgccccg ctgatcttcc tgggcctggt ggccgcgggc
     1081 ccggccggat ggaaattgcg ggaacgcctc cgggcatcgc gcccttttcc ggcgccgccc
     1141 gggccgctct tgggcgcctt gacggcggcc gccctggccg cttgggccct ggggttcctg
     1201 caggccttcc tgcccgagac cttctacgat tcgctcaatt atttcctagg ggtgccccac
     1261 ttctgggccc tgcggcacgg cgtctgcgac gagccgggcc agatcctctc gggctatttc
     1321 cacggcggca cccttttcct gatggacggt ttttatttcg gcggcgccgg cgccgccaag
     1381 atgctggctt ttttctccgc cttcgcctgt gcggccttcg ccggcgcctg ggcgggggag
     1441 gaggcgggtg ttggggccgg ttggacggcc ttcggcctga cggccgcctt ccccttgttg
     1501 gccctcaacg gatgggccgc ccgcgtggac gggctgctca ccttctgcct gctggccttc
     1561 ttttacgcct gccgaaaatg cttcccggcc gccgccccgg gctggggcgc ggcggcgggg
     1621 ctcttgggcg gttacgccgt gacggtcaaa cccacggcgc tggtggggat cgcggccgtc
     1681 ctgctttgcc tgttgcccgc ctggcggcgc gcgcctgggc gcgcgtggct cacgcccctg
     1741 gcgctgggcg cgggcgtggt ggcgccctgg ttggccaaga acgcctgctt ca
//
LOCUS       DASTJJ010000719         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1077168, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000719 DASTJJ010000000
VERSION     DASTJJ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077168"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..735)
                     /locus_tag="VFR02_08710"
     CDS             complement(31..735)
                     /locus_tag="VFR02_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET9870560.1"
                     /translation="MCGRYVVARATADLVGMFDVAEVGEGLPGPAWNIKPTNRVPVVL
                     ESAKGTDTAVRRLEAARWSLTPSYSKELKTKFATFNARAETVGEKATFRGSVKSKRAI
                     LPADYYYEWHTEGKTKTPYAIHSPNNEVLAFAGLYTWWPDKALPEDHNDYWTLTATIL
                     TRAAVGAVSELHDRTPVTLPPSFWDEWLDAGQDGDQSLVDAAVAAATPVAEALEFYQV
                     GPLTGNGPELVHPTVS"
BASE COUNT          169 a          360 c          342 g          176 t
ORIGIN      
        1 cattgggagg agttgcccgt ggccagggcc tcatgaaacg gtggggtgta ccagttcggg
       61 cccgttgccg gtgagcgggc cgacctggta aaactccaac gcctcggcca ccggggtggc
      121 ggcggcgacg gcggcgtcga ccagggactg gtcgccgtcc tggccggcgt ccagccattc
      181 gtcccaaaag cttggtggca gggtcacggg tgtgcggtcg tgcagctcgc tcacggcgcc
      241 caccgctgcc cgggtcagga tcgtggccgt cagtgtccag tagtcattgt ggtcctcggg
      301 cagggccttg tccggccacc acgtgtacag gccggcaaac gccaggacct cattgttggg
      361 ggagtggatc gcgtacgggg ttttcgtctt cccttccgtg tgccactcgt agtagtaatc
      421 ggccggcagg atcgcccgct tgcttttcac cgatccccgg aaggtcgcct tctcgccgac
      481 cgtttcggcc ctagcgttga aggtggcgaa cttcgtcttc aactccttgg aatacgaagg
      541 cgtcagcgac caccgggcgg cctccagccg gcgcacggcc gtgtccgtgc ccttggcgga
      601 ttcgagcacc accggcaccc ggttggtggg cttgatgttc cacgccggcc ccggcaggcc
      661 ctcgccgacc tcggccacat cgaacatgcc caccaggtct gcggtcgccc gggccaccac
      721 gtatctccca cacatgaagc ccattctgcc ccggcagccc gccagccgga agccgcggcc
      781 gtcaaaaaag ccatacccag gccggccagg gccgggcacg aggtcatgat ccccgacacg
      841 acacggcggt cccttcgggt cgtgcaatgg ctgtcgatag tccgtacact cgtcactaca
      901 gtggccagcc ggccccccca atacggctgg ccaccctgcc gtttaaggac aacaaccccc
      961 aaagacggtt acaggcagca tggacgaccc cttggggtca cggggaaggg tcgggcgggc
     1021 acagaaaccc ttggccgggg cgccggg
//
LOCUS       DASTJJ010000720         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1810073, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000720 DASTJJ010000000
VERSION     DASTJJ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810073"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFR02_08715"
     CDS             complement(<1..673)
                     /locus_tag="VFR02_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9870561.1"
                     /translation="MASLSPRQLNAFLSAARTAARAGGARALRYFRKNVPVIRKPDRS
                     PVTRADREAEQVIRGLLGRRFPGHQLCGEEFGWEEGQKAEFRWWIDPVDGTRQFIRGI
                     PYWGTLLALEWRGDVVAGVIHFPAVGSTLWASRGRGCYADGKRVRVSKIKRLAHGTLT
                     YGGLRLTPRSYRKKLTDLIEGCYDDRGFGDCFGHSLVIQGKAEAMVDPVVKPYDVAAV
                     KICLEE"
BASE COUNT          203 a          333 c          291 g          187 t
ORIGIN      
        1 cctcttccag gcagatcttg accgccgcca cgtcgtaggg cttcaccacc gggtccacca
       61 tggcctcggc cttgccctgg atgacgagcg aatgcccgaa acagtccccg aagccccggt
      121 cgtcgtagca tccctcgatc aggtccgtca gtttcttgcg gtaggaacgc ggcgtgagcc
      181 tcaggccccc gtaggtcaag gtgccgtggg ccaggcgctt gatcttggag acccggaccc
      241 gtttgccgtc ggcgtaacaa ccccggcccc gggacgccca caaagtggat cccaccgcgg
      301 gaaagtggat cacccccgcc accacgtcgc cccgccattc cagggccaac agggtgcccc
      361 aataggggat gccccggatg aactgccggg tgccgtccac cggatcgatc caccagcgga
      421 actcggcctt ttgcccttcc tcccacccga actcctcgcc gcaaagctgg tgccccggga
      481 accgacgccc caagagccca cggatgacct gctcggcctc ccggtcggcc cgggtgaccg
      541 gcgagcggtc gggtttgcgg atgaccggca cgttcttgcg gaaatagcgc agggcccggg
      601 ccccgcccgc gcgggcggcc gttcgggccg ccgacaggaa agcgttcaat tgacgggggg
      661 acagggacgc catgggggcc tcgggacttg gaatggccgc attctaccct tccccgcccc
      721 aaaataaaaa cggaactatg gcttttgcaa agcaagccta agagaacttg aaacaatgat
      781 aaaaactgat gaaatctgaa tttttggaaa taaaaatcgg gctttatcag cttttatcaa
      841 tgttccagtt gtccttgctt tgcgaattgc atgattccga ataaaaacgg aactatgtgt
      901 tcttagcgcg agaagtttgg acaaatcctc cgtctgaacc ggcccttcgt caagcggcct
      961 gagggcccac ctcctgcgag gccttcgtca gctgggcctc catggtttgc cacg
//
LOCUS       DASTJJ010000721         1344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_587652, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000721 DASTJJ010000000
VERSION     DASTJJ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587652"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFR02_08720"
     CDS             <1..746
                     /locus_tag="VFR02_08720"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET9870562.1"
                     /translation="PHQVCFVMDAGGFGRIREGVRRSEFATLVNLDHHYSNDHYGDYN
                     LVVPKASATGEVVYHLIRALGVAVDARIAESVYTSLVTDTGGFRYRNTTPHVLRLAAD
                     LVEAGAEAQKVCDRIFAGVTRQAMDLARISLGHTRFFDGGAIGSMTLTQADLKRSGAT
                     DDDTENLINYVRKIHTVKVAVFLKERPDGRLKLSLRSRSPKVNVADIAAVFGGGGHSY
                     AAGALLTGPLSGALKRVITVCRRFLKRSQ"
     gene            880..>1344
                     /locus_tag="VFR02_08725"
     CDS             880..>1344
                     /locus_tag="VFR02_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9870563.1"
                     /translation="MDGLLILDKPAGWTSHDVVAKARGLLKERQIGHLGTLDPLATGV
                     LPLALGAATRLIEFAPHDKEYLAGCLLGRSTDSEDVTGRILGEASTDQLEEGAVREAV
                     LALGRLTEQVPPMVSAIKKDGQKLYELARAGKTVEREPRPIRIREVEVVSLEL"
BASE COUNT          234 a          461 c          423 g          226 t
ORIGIN      
        1 ccccgcacca ggtctgcttc gtcatggacg cgggcggctt cgggcgcatc cgcgagggcg
       61 tgcggcgctc cgaattcgcc accctggtga acctggacca ccattattcc aacgaccatt
      121 acggcgacta caacctggtg gtgcccaagg cctccgccac cggggaagtg gtctaccacc
      181 tgatccgcgc cctgggcgtg gcggtggacg ccaggatcgc cgaaagcgtc tacacctcgc
      241 tggtcaccga cacgggcggg ttccgctacc gcaacaccac cccccacgtg ctgcgcctgg
      301 ccgccgacct ggtggaggcc ggcgccgagg cccagaaggt ctgcgaccgc atcttcgccg
      361 gggtcacccg gcaggccatg gacctggccc gcatctcgct gggccatacc cgtttcttcg
      421 acgggggcgc catcgggtcc atgaccctca cccaggccga cctgaagcgc tcgggcgcca
      481 ccgatgacga caccgagaac ctcatcaatt acgtgcgcaa gatccatacg gtcaaggtgg
      541 cggtgttcct gaaagaacgc cccgacggac gcctcaagct ctccctgcgc tcccgttccc
      601 ccaaggtcaa cgtggccgac atcgccgcgg tcttcggcgg gggcgggcat tcctacgccg
      661 ccggggcgct cttgaccggc cccttgagcg gggccctgaa gcgcgtgatc accgtctgcc
      721 gccgttttct gaagcgatcc cagtaaagcc attttcgcca cgaagtgcac gaagttcaca
      781 aagttaaaat tttaggaccc atcgaacgag cttttggcct tacttcgtgt acttggtgga
      841 cttcgtggtg aatatgagtt catcctttta aaggccccca tggacggatt gctcatcctg
      901 gataaacccg ccggttggac ctcccacgac gtggtggcca aagcgcgcgg gctcttgaag
      961 gaacgccaga tcggccacct gggcaccttg gatcccctgg ccaccggggt gctgcccctg
     1021 gcgctcggcg ccgccacccg gctcatcgag ttcgcccccc acgacaagga atacctggcc
     1081 ggctgcctgt tgggccgctc cacggacagc gaggacgtga ccggcaggat cctgggcgag
     1141 gcctccaccg atcagttgga agagggcgcg gtgcgggagg cggtcctggc cctgggccgg
     1201 ctcaccgagc aggtgccgcc catggtgtcc gcgatcaaga aggacgggca aaaactttac
     1261 gagctggccc gggctgggaa gaccgtggaa cgggaacccc ggcccatccg catccgggag
     1321 gtggaagtgg tgtcgctgga gctc
//
LOCUS       DASTJJ010000722         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_466788, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000722 DASTJJ010000000
VERSION     DASTJJ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466788"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFR02_08730"
     CDS             complement(<1..363)
                     /locus_tag="VFR02_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870564.1"
                     /translation="MKANDPRKGGRPLPSLVQRLWAVWRRHFAVYWRNFFTNSFYIVV
                     EPVIFITAVGWGLATSIGGMDGMDYLSFIAPGQVMVAAVFSAAFDMSYGTYFRMEMDH
                     NYDSMLVTPLGVTDIFWGE"
     gene            complement(350..1255)
                     /locus_tag="VFR02_08735"
     CDS             complement(350..1255)
                     /locus_tag="VFR02_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9870565.1"
                     /translation="MPANLIEAKDLCKAYGGFQAVDGVDFAVRAGECFGFLGPNGAGK
                     STLMKMIYCFSPRSSGDLSVLGLDPDRSPAPLKNRLGVVAQSDNLDDEITVLENLKVY
                     SGYFGLPRRQAAAKIAELLGFMSLTEKAAARIRELSGGMKRRLVIARALLNDPDLLIL
                     DEPTTGLDPQVRHLIWVKLRELKRRGVTLLLTTHYMEEAQQLCDRLLVMDRGKVLAQG
                     RPRGLVKKYLPPYVLELPAAKRPARVPAGVRIEKYQDRVFLYSASPASLHRAAGTVRG
                     HFQIRPTGLEDLFLKLTGRDLHESE"
     gene            complement(1314..>2252)
                     /locus_tag="VFR02_08740"
     CDS             complement(1314..>2252)
                     /locus_tag="VFR02_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9870566.1"
                     /translation="SGEEAGMELRTQVEGQTPAKFRQNGLDYDIRVRVKDDQQDLEKD
                     FDRVLVPNQNNDLVRLSDIAKPVTTEGPAKINRQDRARYIMITGQLGKDGNLGNILAD
                     AKKVMAGIQLPPGVRYEFVGQAEDYRDLGVSMMVAVGLALLFTYMILASLYESPILPL
                     TIMMAIPLAIVGAFFALFITGQSLNVFSWISIIMLLGLVTKNSILLVDYTLQMQKKGH
                     SREESLKLAGKVRLRPILMTTSSLVMGMLPLALALTEVGKFRQSMGVAVEGGLLSSLV
                     LTLFIVPSIYGYMDDLRLFTRRLLGLDAAKPAPRKR"
BASE COUNT          406 a          709 c          750 g          387 t
ORIGIN      
        1 ctccccccag aagatgtcgg tgacgcccaa gggcgtcacc agcatggaat cgtaattgtg
       61 gtccatctcc atgcggaaat aggtgccgta ggacatgtcg aaggccgccg agaagaccgc
      121 cgccaccatc acctggccgg gcgcgatgaa ggaaaggtag tccatgccgt ccatgccgcc
      181 gatggaggtg gccaggcccc agcccaccgc cgtgatgaag atgaccggct ccaccacgat
      241 gtagaacgaa ttggtgaaga agttgcgcca atagaccgcg aaatggcgcc tccagacggc
      301 ccaaagcctc tggaccaggg acggcagggg ccgccccccc ttccgggggt cattcgcttt
      361 catgcaggtc cctcccggtg agtttcagga aaaggtcctc gagcccggtg ggcctgatct
      421 ggaaatgccc ccggaccgtc ccggccgccc ggtgcagcga ggcgggcgag gccgaataga
      481 ggaagacacg gtcctggtac ttttcgatcc ggacgcccgc cgggacccgg gccgggcgtt
      541 tggcggcggg aagttccaac acgtagggcg gcaggtattt tttcaccaga cccctgggcc
      601 tgccctgggc caacaccttg ccccggtcca tcaccaagag ccggtcgcag agctgctggg
      661 cctcttccat gtaatgcgtg gtcaagagca gggtgacgcc ccgccttttg agctcccgca
      721 gcttcaccca gatcaggtgg cgcacctggg ggtccaaccc cgtggtgggc tcgtccagga
      781 tgagcaggtc cgggtcgttc aaaagggccc gggcgatgac caggcgccgt ttcatgccgc
      841 ccgacaattc ccggatgcgg gcggcggcct tttcggtgag cgacatgaag ccgagcagct
      901 cggcgatctt ggcggcggct tggcggcgcg gcaggccgaa ataacccgag tagaccttga
      961 ggttctccaa cacggtgatc tcgtcgtcca ggttgtcgga ttgggccacc acgcccaggc
     1021 ggttcttgag gggggcgggg gatcggtccg ggtccaggcc caggacgctc aggtccccgg
     1081 agcttcgcgg ggagaaacaa tagatcatct tcatcagggt ggatttgccc gcgccgttgg
     1141 ggcccaggaa cccgaagcat tcccccgccc ggacggcgaa gtccacgccg tccacggcct
     1201 ggaagccgcc gtaggctttg cagaggtcct tggcttcaat gaggttggcc ggcatggttc
     1261 cctccccggg aatggggcgc cagcctagcc taaaaacgcg gtgaaggaaa gggtcagcgc
     1321 ttcctgggcg cgggcttggc cgcgtccagc cccagcaggc ggcgcgtgaa gagccgcagg
     1381 tcgtccatgt agccgtagat ggacggcacg atgaagagcg tgaggaccag ggagctcagg
     1441 agcccgccct ccaccgccac gcccatggac tggcggaact tgcccacttc ggtcaaggcc
     1501 agggccagcg gcagcatgcc catgaccagc gacgaggtgg tcatgaggat gggcctcaaa
     1561 cgcaccttgc cggccagttt cagggactcc tcgcggctgt ggcccttttt ctgcatctgg
     1621 agcgtgtagt cgaccaggag gatggagttc ttggtgacca gccccagcag catgatgatg
     1681 gagatccaac tgaagacgtt cagcgactgg cccgtgatga agagcgcgaa gaaggccccc
     1741 acgatggcca gggggatggc catcatgatg gtgaggggca ggatgggcga ctcatagagg
     1801 ctggccagga tcatataggt gaagagcagg gccaatccca cggccaccat catgctcacc
     1861 cccaggtccc ggtaatcctc ggcctggccc acgaactcgt aacgcacgcc gggcggcagc
     1921 tggatgcccg ccatgacctt cttggcgtcg gccaggatgt tgcccaggtt gccgtccttg
     1981 cccaattggc ccgtgatcat gatgtagcgc gcccggtcct gccggttgat cttggccggg
     2041 ccctcggtgg tgaccggctt ggcgatgtcg gacaggcgca ccaagtcgtt gttctggttg
     2101 ggcaccagga cacggtcgaa gtccttttcc aagtcctgct ggtcgtcctt cacccgcacc
     2161 cggatgtcgt agtccaggcc gttctggcgg aacttggcgg gcgtctggcc ctccacctgg
     2221 gtcctgagtt ccatgccggc ctcctcgccg ga
//
LOCUS       DASTJJ010000723         1208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1322494, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000723 DASTJJ010000000
VERSION     DASTJJ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322494"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFR02_08745"
     CDS             complement(<1..533)
                     /locus_tag="VFR02_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET9870567.1"
                     /translation="MASPDVKRYLQNWQGEVDGAAQYHALARAEKNPKLSQVYANLAR
                     MEEKHRALWESKLREAGTPVPAPRPSWRARALIFLTERFGPGAVLSTVAEGERADRNM
                     YVAQAEAQGTSLTADEHLHDRVLEEILRVDKQGVSGDYLAKLEGRHKAVGGNNLRAAV
                     LGANDGLCSNLSLVMGVA"
     gene            857..>1208
                     /locus_tag="VFR02_08750"
     CDS             857..>1208
                     /locus_tag="VFR02_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET9870568.1"
                     /translation="MYVPSSAELYREFFSRLNIFKSRKNWLALFSWLVVLFFVPFFFL
                     FFFHYFTWPLLLVGFVYSMVVLGTHGTVYLHRYSTHHAFTLSNRLWLFILRNLVIKIV
                     PEEAYVVSHHVHHFL"
BASE COUNT          184 a          437 c          333 g          254 t
ORIGIN      
        1 gccacgccca tcaccaggct caggttcgaa caaagcccat cgttggcccc caggaccgcc
       61 gcccgcaggt tgttgccccc cacggcctta tgccggccct cgagcttcgc cagatagtcc
      121 cccgacaccc cctgcttgtc cacgcgcagg atctcctcca acacgcggtc atgcaggtgt
      181 tcgtcggcgg tcagggaggt gccttgggcc tcggcctgcg ccacgtacat gttgcggtcc
      241 gcccgctcgc cctccgccac cgtgctcagg accgcgcccg ggccgaaccg ttcggtgagg
      301 aagatcagcg cgcgggcgcg ccaactggga cgcggagcgg ggacgggcgt gcccgcttcc
      361 cggagcttgg attcccacag ggcgcggtgc ttctcctcca tgcgggccag gttggcgtag
      421 acctgggaca acttggggtt cttctcggcg cgagccaggg catggtactg ggccgccccg
      481 tccacctcgc cctgccagtt ctgcaggtac cgcttcacgt ccggcgacgc catggcgtcc
      541 tccttgtggt cgaagtggcc ctaccttaca agaagcgcgg cgcgaattca gccgtccggc
      601 ccccgggaaa accttatccc cgctgttccc cttcggaata aaggaccgga cggcggaaaa
      661 gggccacgga ttcccgcttt tcacggccgg ctttccaccg aattaaccct atccccctcc
      721 gggaggggat atactggccg ggtcgataat gaaatgaccc aagccctccc ggggccgcgt
      781 gtcgaacgga aaccctttgg ccttcctaaa ccgtgttttg gacccccctt cctatggcta
      841 tgtccggaac ggacagttgt acgtcccctc ctccgccgag ctttaccgcg agttcttcag
      901 ccggctcaac atcttcaagt cccgcaagaa ttggttggcg cttttcagct ggctggtggt
      961 gctctttttc gtccccttct tcttcctttt tttcttccac tacttcacct ggcccctgct
     1021 gctcgtgggg ttcgtctaca gcatggtggt gctgggcacc cacggcacgg tctacctgca
     1081 ccgctattcg acccaccacg ccttcaccct gtccaaccgc ctctggctct tcatcctgcg
     1141 caacctggtg atcaagatcg tccccgagga agcctacgtg gtgtcccacc acgtgcacca
     1201 tttcctgt
//
LOCUS       DASTJJ010000724         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_587810, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000724 DASTJJ010000000
VERSION     DASTJJ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587810"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFR02_08755"
     CDS             <1..669
                     /locus_tag="VFR02_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9870569.1"
                     /translation="DFAGVIPPDQAGQIATLLTELEQKTGAQVAVVTLPSLNGADIDS
                     TAADLFRQWGIGHKKKDDGVLILAAVQDHKIRIEVGYGLEPILPDGEAGSIIRRYMAP
                     QFKQGDYGSGFLVGSVAVAQLIAQNAGVTLTGKAMDLPLSEGDNPYGQLSFWAFLLIF
                     LSLWAVIAWAGRRQRGSRWGGGYWGGGYWGGGFGGGGFGSGGGGFGGFGGGASGGGGA
                     SGGW"
     gene            688..1389
                     /locus_tag="VFR02_08760"
     CDS             688..1389
                     /locus_tag="VFR02_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870570.1"
                     /translation="MAKWTVEQFKDELQKTLGPGLISLVLYGSQAAGDHLGEASDTNL
                     LALVDRLDLDRLKALSGPVAAWAKQGNPPPLFLTPGHLRRYVDLFPLEISDIQENHRV
                     LAGTDPFEGLVNGRTALRQQLEHEFQGKLLRLRTRFLLTRGREGELRKLLAGSLSSFL
                     VLLKNALWLYGEKPPVKKLEAVKRLGERLGLPTTVFGTVELLKRGERIPKLSLETLFG
                     EYLRVLESFQDKIHG"
     gene            1382..1945
                     /locus_tag="VFR02_08765"
     CDS             1382..1945
                     /locus_tag="VFR02_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9870571.1"
                     /translation="MASRVPWLGGALALLALGALAAGAWAKASPVHPHLRLLSKAFGD
                     GNKLPVRYTCDGAGVSPELHWKNLPRKTKSLALILDDPDAPYSTWIHWVAYNIPPDVL
                     EFLEDFPKDAQLPNGITQGTNDFKTLGYGSPCPPSGVHRYFFRLYALDSRLDLAPGAT
                     AEQLRQAMKGHILSMAQLMGRYGQYER"
     gene            1964..2542
                     /locus_tag="VFR02_08770"
     CDS             1964..2542
                     /locus_tag="VFR02_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9870572.1"
                     /translation="MPDSNWNMKWMLGLAGFLLLVFLICMGSYNSLVSANEAVEAQWS
                     QVENVMQRRFDLIPNLVQTVKGYAKHERETLEEVTRLRSQWGAAQTREQKVAASDQLE
                     GTLNHLMVVLENYPNLKANENFLRLQDELEGTENRIAVERRRYNDDLRAYNVKVRSFP
                     SDIMASLGGFQRKDAYFKASPGADQAPQVDFN"
     gene            complement(2539..2739)
                     /locus_tag="VFR02_08775"
     CDS             complement(2539..2739)
                     /locus_tag="VFR02_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9870573.1"
                     /translation="MERILFIGAAVLLIFGPEKVPEIARALGNLTREFQNALHPPPEA
                     PAAAPAPKRPAARRRRARRRSR"
BASE COUNT          482 a          897 c          990 g          470 t
ORIGIN      
        1 gatttcgccg gggtcatccc ccccgaccag gccggccaga tcgccaccct gctcaccgaa
       61 ttggaacaaa agaccggcgc ccaagtggcc gtggtgaccc tgcccagcct caacggcgcc
      121 gacatcgact ccacggccgc cgacctgttc cgtcagtggg gcatcggcca caagaagaag
      181 gacgacggcg tgctcatcct ggccgccgtc caggaccata agatccgcat cgaggtgggg
      241 tacggcctgg aacccatcct ccccgacggc gaggcgggct ccatcatccg ccgctacatg
      301 gcgccccagt tcaagcaggg cgactacggc agcggattcc tggtggggtc ggtggccgtg
      361 gcccaattga tcgcccagaa cgcgggcgtg accctgaccg gcaaggccat ggacctgccg
      421 ctctcggagg gcgacaaccc ctacggccag ttgtccttct gggccttcct gctcatcttc
      481 ctttccctct gggcggtcat cgcctgggcg ggccggcgcc agcggggaag ccgctggggc
      541 ggcggttatt ggggcggcgg ctattggggg ggcggtttcg gaggcggtgg tttcggttcg
      601 ggcggcgggg gattcggcgg tttcggcggc ggcgcctcgg gcggcggcgg cgccagcggc
      661 ggttggtaac ggataaggag aagggtcatg gccaaatgga cggtcgaaca attcaaggat
      721 gagcttcaaa aaaccctggg gcccgggctg atcagcctgg tgctctacgg ctcccaggcg
      781 gcgggggacc atctggggga ggcctcggac accaacctgt tggccctggt ggaccggttg
      841 gacctggacc ggctgaaggc cctctccggg cccgtggcgg cctgggcgaa acaaggcaac
      901 ccgccgccgc tcttcctcac ccccggccac ctgcggcggt acgtggacct cttccccctg
      961 gaaatttccg acatccagga gaaccaccgg gtgctggcgg gcacggaccc cttcgagggg
     1021 ctggtgaacg gccggaccgc cctgcggcag caactggaac atgagttcca ggggaaactg
     1081 ctgcgcctgc gcacccgctt cctgctcacc cggggccggg agggggaatt gcgcaagctc
     1141 ttggccgggt ccctgtcgag cttcctggtg ttgttgaaga acgccctctg gctttacggc
     1201 gaaaagccgc ccgtgaagaa gctggaggcg gtgaaacgcc tcggcgagcg gctggggctg
     1261 cccaccacgg tcttcggcac ggtggaactg ttgaagcggg gggagcgcat cccgaaactc
     1321 tcgctggaaa cccttttcgg cgaatacctg cgggtcctgg aatccttcca ggacaagatc
     1381 catggctagc cgggtcccct ggctgggggg cgcgctggcc ctgctggcgc tgggggcgct
     1441 ggcggccggc gcctgggcca aagcttcccc tgtccatccc cacctgcggc tattgtccaa
     1501 ggcctttggg gatgggaaca agctgccggt gcggtacacc tgcgacgggg ccggcgtttc
     1561 gcccgagctg cattggaaga acctgccccg taagaccaag agcctggccc tcatcctgga
     1621 cgacccggac gcgccctatt ccacctggat ccattgggtg gcctacaata tccccccgga
     1681 cgtgctggag ttcctggagg acttccccaa ggacgcccaa ttgcccaacg gcatcaccca
     1741 gggcaccaac gacttcaaga ccctgggcta cggaagcccc tgcccgccca gcggcgtgca
     1801 ccggtatttt ttcaggcttt acgcgttgga cagccggctg gacctggcgc ccggggccac
     1861 ggcggagcag ttgagacagg ccatgaaggg ccatatcctg tccatggccc agctcatggg
     1921 gcgttatggg caatacgaac gttgaggaaa aaaggagggt tcgatgccgg attcgaattg
     1981 gaacatgaaa tggatgctgg ggttggcggg gttcctgctg ctggtcttcc tcatctgcat
     2041 ggggtcctac aactcgctgg tctcggccaa cgaggcggtg gaggcccagt ggagccaggt
     2101 ggagaacgtg atgcagcggc gcttcgacct catccccaac ctggtgcaga ccgtcaaggg
     2161 ctacgccaag catgagaggg agaccctgga ggaggtgacc cgcctgcgca gccagtgggg
     2221 cgcggcccag acccgggaac aaaaggtcgc ggcctcggac cagctggaag gcaccctgaa
     2281 ccacctgatg gtggtcctgg agaactaccc aaacctgaag gccaacgaga atttcctgcg
     2341 gctccaggac gagctggagg ggacggagaa ccgcatcgcg gtggagcgcc ggcgctacaa
     2401 cgacgacctg cgggcctata acgtgaaggt gcgcagcttc ccctcggaca tcatggcgtc
     2461 cctggggggg ttccagcgca aggacgccta cttcaaggcg tcgcccgggg ccgaccaggc
     2521 gccccaggtc gacttcaact aacggctccg ccgccgggcg cggcgccggc gggcggcggg
     2581 tcttttcggg gccggggcgg cggccggggc ctcggggggc gggtgcaggg cgttctggaa
     2641 ttctcgggtg aggttgccca aggcccgggc gatctcgggc accttttcgg gaccgaagat
     2701 caagagcacc gccgcgccga tgaagaggat gcgttccatg gtttttcccc cttgggtccc
     2761 ttgttataaa gggtccgggg ggcggattgc aaatcattcc aagcgaggcg cgcatgggcg
     2821 tggggggtgt cctttggct
//
LOCUS       DASTJJ010000725         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_710788, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000725 DASTJJ010000000
VERSION     DASTJJ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710788"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFR02_08780"
     CDS             <1..492
                     /locus_tag="VFR02_08780"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET9870574.1"
                     /translation="LILMGVSGSGKTTLGRKLAEAIGCPFFDGDDLHPPANREKMAQG
                     TALNDADRAPWLAELARAIGEWNRDRPLTVLACSALKASYREKLGAGGGVRWIHLKGD
                     RALIAHRLKGRSGHFFPPALLDSQFHDLEEPEDAWTADITQEPDTIVAAILRYLRETR
                     VLR"
     gene            479..676
                     /locus_tag="VFR02_08785"
     CDS             479..676
                     /locus_tag="VFR02_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870575.1"
                     /translation="MYYGDSGKMIWIVFLTIILVLAGFYWHQSWFAVRFLAQLVFLNL
                     PPHLQEWLSGFFGHSPDPGTP"
     gene            795..>1221
                     /locus_tag="VFR02_08790"
     CDS             795..>1221
                     /locus_tag="VFR02_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9870576.1"
                     /translation="MKFSALDEYGLRCMLQMARKGHPGTMTIVELAQREALTPAYVGK
                     LMTLLRKGGLVQSIRGQAGGYQLARPPREISVNEVLEVLGGKFYSREEDCQKFPGQHE
                     SCVHSMDCSIRSLWTGLDRVISGYLSRSKLSDLVMSEPEM"
BASE COUNT          231 a          354 c          385 g          251 t
ORIGIN      
        1 ctgatcctca tgggggtctc cgggagcggg aagacgacct tgggccggaa gttggcggag
       61 gcgatcggct gtcccttttt cgacggcgac gatctccatc cccccgcgaa ccgggaaaag
      121 atggcccaag gcaccgcctt gaacgacgcc gaccgggcgc cctggctggc cgaactggcc
      181 cgggccattg gggagtggaa ccgcgaccga ccgctcactg tgctggcctg ttcggccttg
      241 aaggcctcct accgggaaaa attgggagcg ggcggtgggg tccgctggat ccatctgaag
      301 ggggaccggg cgttgatcgc ccacaggctc aagggccgat cggggcattt tttccctccc
      361 gcgctcttgg acagccaatt ccacgacctg gaagaaccgg aagacgcgtg gaccgccgac
      421 atcacgcagg aacctgatac aatagtggca gccatcctcc ggtacctgag ggaaacccgt
      481 gtattacggt gacagcggca aaatgatctg gatcgtgttc ctgaccatca tcctggtcct
      541 ggcggggttc tattggcacc agagctggtt cgccgtccgc ttcctcgccc aattggtttt
      601 cttgaacctg ccgccccatc tccaagagtg gttgtcgggt ttctttggcc attccccgga
      661 tcccgggacc ccctgaagtc cttttgggtt gaactttgcc ataaaattca tatacttgac
      721 tttttcgtca agattgccgg atgatggttc aaaggggtga aagcccccaa acccggttaa
      781 aaggccttga tttcatgaag ttttcggcgc tggatgaata tggtttgagg tgcatgctcc
      841 aaatggcccg caaaggccat ccgggcacca tgaccatcgt ggaattggcc cagcgggaag
      901 ccctgacccc cgcctatgtg ggcaaactga tgaccctgct gcgcaagggg gggctggtcc
      961 agagcatccg ggggcaggcc ggcggctatc aattggcgcg cccgccccgg gagatcagcg
     1021 tgaacgaggt gctggaggtc ctgggcggca agttctattc ccgcgaggag gactgccaaa
     1081 agttcccggg ccagcatgag tcctgcgtgc attcgatgga ctgttccatc cgctcgcttt
     1141 ggaccgggtt ggaccgggtg atctcgggct atttgagccg cagcaaattg tccgacctgg
     1201 tgatgagcga gcccgagatg g
//
LOCUS       DASTJJ010000726         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_467456, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000726 DASTJJ010000000
VERSION     DASTJJ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467456"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /gene="hemA"
                     /locus_tag="VFR02_08795"
     CDS             <1..919
                     /gene="hemA"
                     /locus_tag="VFR02_08795"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9870577.1"
                     /translation="VKDAFRSAVEAGSAGAVLTGIFNRAFAAAKAVRSQTEIARMPVS
                     VSSVAVGLAGKIFGSFENKKVLLLGAGETGELTAKYLADAGVTDFRVANRTVEKAQAL
                     AARLGGWGGSLDEGLQALGEADILVASLQLDRPLVSAQDVERSLKARKGRPLFIIDLG
                     VPRNVEGEAGKLPQVYLYNIDDLSGLAQANQEGRQKAIAAADAILDREIAELCSWLNN
                     LELVPTIVRLRERFERIRQGEWDDFSRRLAHLGEKDRALVERLSRDLTAHLLHDPLVT
                     LKNLPDARERFEYAKMLNDLFKLWKGPEA"
     gene            916..>1439
                     /gene="hemC"
                     /locus_tag="VFR02_08800"
     CDS             916..>1439
                     /gene="hemC"
                     /locus_tag="VFR02_08800"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9870578.1"
                     /translation="MTPLGVGTRGSALALAQAGQVLAQMKACLSQPLALEVIKTSGDR
                     FSASDAPDTHLDATVQGLFTKELDEALLQGRIRAAIHSLKDLPTVLTPGLRYGCFLER
                     GDFRDALISRDGKKFMGLPPGARVGTGSPRREAQVRAARPDLAVAPLRGNLDTRLRKL
                     AEGLYDAVVVAGAGV"
BASE COUNT          225 a          483 c          516 g          215 t
ORIGIN      
        1 ggtgaaggac gccttccgtt cggcggtgga ggccgggtcg gcgggggccg tgctgaccgg
       61 catcttcaac cgggccttcg cggcggccaa ggccgtgcgc agccagaccg agatcgcccg
      121 catgccggtc agcgtttcca gcgtggcggt gggcctggcg ggcaagatct tcggcagctt
      181 cgagaacaag aaggtgctgc tcctgggggc gggggagacg ggcgaactca cggccaagta
      241 cctggccgac gccggcgtga cggatttccg ggtggccaac cgcacggtcg aaaaggcgca
      301 ggccctggcc gcccgcctcg gcggatgggg cgggtccttg gacgaggggc tgcaggccct
      361 gggagaggcg gacatcctgg tggcctccct ccagttggac cggccgctgg tctcggccca
      421 ggacgtggaa aggtcgctca aggcccgtaa gggaaggccc ctcttcatca tcgacctggg
      481 ggtgccccgc aacgtggagg gggaggcggg caagctgccc caggtgtacc tctacaacat
      541 cgacgacctc tcgggcctgg cccaggccaa ccaggaaggg cgccaaaagg ccatcgcggc
      601 cgccgacgcc atcctggacc gggagatcgc ggaactttgt tcctggttga acaacctgga
      661 actggtgccc accatcgtgc gcctgcggga acggttcgaa cgcatccgcc agggcgaatg
      721 ggatgatttt tcccgccgcc tggcccacct gggtgaaaag gaccgggccc tggtggagcg
      781 cctgagccgg gacctgaccg cccacctgct gcatgacccg ctggtgaccc tcaagaacct
      841 gccggacgcc cgggagcggt tcgagtacgc caagatgctc aacgacctct tcaagctttg
      901 gaaggggccc gaggcgtgac gcccctcggg gtgggcaccc gcggcagcgc actggccttg
      961 gcccaggccg ggcaggtgct ggcccaaatg aaggcctgcc tgtcccagcc cctggcgctg
     1021 gaggtcatca agaccagcgg cgaccgtttt tccgcctccg acgccccgga cacccatctg
     1081 gacgccacgg tccagggcct cttcaccaag gaattggatg aggcgctctt gcagggccgc
     1141 atccgcgccg ccatccacag cctgaaggac ctgcccacgg tcctgacccc gggtctccgt
     1201 tacggctgct tcctggagcg gggggatttc cgggacgcgc tgatttcccg ggatgggaaa
     1261 aaattcatgg gcctgccccc gggggcgcgc gtgggcaccg gctccccccg ccgggaagcc
     1321 caggtgcggg ccgcccggcc cgacctggcg gtggcccccc ttcggggcaa tttggacacc
     1381 cgcctgcgca agctcgccga gggcctttat gacgcggtgg tggtggcggg ggcgggtgt
//
LOCUS       DASTJJ010000727         1316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1689637, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000727 DASTJJ010000000
VERSION     DASTJJ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689637"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="der"
                     /locus_tag="VFR02_08805"
     CDS             complement(<1..1142)
                     /gene="der"
                     /locus_tag="VFR02_08805"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9870579.1"
                     /translation="MPLPQVLIVGRPNVGKSTFFNRLTGKSLALVDPQPGSTRDLKNH
                     DVEWNGSFFTLFDSGGWVPGDEGSIASKIGSQLERKARQVGALLFLVDALDGVTPADE
                     LVARNLRKYGVPTLLVVNKADQFEKRHEALADFLKLGFDHAFPISASHGLGIDDLLDA
                     LIAVLARTAKAPAPDEEGDRPLRFAVMGKPNVGKSSLVNAILGQDRLIVDDFPGTTHD
                     AIPVILETEGDPLVMVDTAGIKSPKQQDSKIEKMSAQQALYELQTCQVVLLLLDGEKG
                     ITHQDVTVSRIIAEAFRPVVVLVNKWDIHPKGAEQANAERIIRRQLRHLPFAPIRFIS
                     AKTGKGLEGLLDLAREVFAESCRQAPTRRVNDVLREASARHAPPFK"
BASE COUNT          204 a          425 c          449 g          238 t
ORIGIN      
        1 tgcttgaagg gcggggcgtg ccgggccgag gcttcccgca gcacgtcgtt gacccggcgg
       61 gtgggcgctt ggcggcagct ttccgcgaag acctcccggg ccaggtccag gagcccctcc
      121 aggcccttgc cggtcttggc ggagatgaac cggatgggcg cgaagggcag gtggcgcaat
      181 tggcggcgga tgatgcgttc ggcgttggcc tgctcggcgc ccttggggtg gatgtcccac
      241 ttgttgacca gcaccaccac gggccggaag gcctcggcga tgatgcggct cacggtcacg
      301 tcctggtggg tgatgccctt ttccccgtcc aacaagagca gcaccacctg gcaggtctgc
      361 agctcataga gggcttgttg ggcgctcatc ttctcgatct tgctgtcctg ctgtttgggc
      421 gacttgatac cggccgtgtc caccatgacc agggggtccc cctcggtctc caggatcacg
      481 ggaatggcgt cgtgggtggt gccggggaaa tcgtccacga tcagccggtc ctggcccagg
      541 atcgcgttca ccagggacga cttgcccacg ttgggcttgc ccatgacggc gaaccgcaag
      601 gggcggtcgc cttcctcgtc gggggcgggg gctttcgccg tgcgggccag cacggcgatg
      661 agggcgtcca acaggtcgtc gatccccagg ccgtgggagg ccgagatcgg gaaggcgtgg
      721 tcgaagccca gcttcaggaa atccgccagg gcctcgtgtc gcttctcgaa ttggtccgcc
      781 ttgttcacca ccagcagggt gggcacgccg tatttgcgca ggtttcgggc gaccagctca
      841 tccgccggcg tgaccccgtc cagggcgtcc accaggaaca gcagggcccc cacctggcgc
      901 gccttgcgct ccaactggga accgatcttg gaggcgatgg acccttcgtc ccccggcacc
      961 caaccgcccg aatcgaacag ggtgaagaag gagccgttcc attccacgtc gtggttcttc
     1021 aggtcccggg tggagcccgg ctgggggtcg accagggcca gggactttcc cgtgaggcgg
     1081 ttgaaaaagg tggacttccc cacgttgggg cgtccgacga tgagcacttg gggcaggggc
     1141 atggcgttcc tttaccgggt cacgacgact ttttgcacgg gcagcttcac caccgacccg
     1201 tcggcgccct cgaaccgggc ctcgtaataa tagatgcctt ccggcacccg gcgcagcttc
     1261 cacaacaggt caaagcaccc gttgccggcc ttcgtcaaca ccgtcacggg cgcgta
//
LOCUS       DASTJJ010000728         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1078976, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000728 DASTJJ010000000
VERSION     DASTJJ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078976"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFR02_08810"
     CDS             complement(<1..599)
                     /locus_tag="VFR02_08810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9870580.1"
                     /translation="MRRRLKVAHLITRLELGGAQQNTLYCCQHHDRKKYEVILICGEG
                     GRLDEEARWIKDCRKYFLSELKHPIHPYWDFLALKRLTEILRLEKVDLVHTHSSKAGI
                     LGRFAAKRAGVPHIIHTVHGWGFHPGQSPAPRLLYQALERWAAGFTDKLIAVSGENKR
                     AGLAARIGRPRQYEVIHSGVDPKRYALDGAAALRARKALG"
     gene            complement(619..>1031)
                     /locus_tag="VFR02_08815"
     CDS             complement(619..>1031)
                     /locus_tag="VFR02_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870581.1"
                     /translation="GQVVNVGGGSPATLLEAVASLEEVSGKACARTFLERQKGDVFST
                     RADTAKLERIFGFKPKVSLKEGLARQWEWMQRFVQNEDEERAIKAAAQPITEVDHAAL
                     AEFKADEKAAKKAARKAKAAKAPPGGDPAPKPDA"
BASE COUNT          132 a          339 c          369 g          191 t
ORIGIN      
        1 cccagtgcct tgcgcgcccg cagggcggcg gccccgtcca gggcgtaacg cttggggtcc
       61 acgccgctgt ggatgacctc gtattgtcgg ggacggccga tgcgggcggc caggcccgcc
      121 cttttgttct ccccgctgac ggcgatgagc ttgtcggtga aacccgccgc ccatcgctcc
      181 agggcctggt agagcaggcg cggcgcgggg gactggcccg gatggaagcc ccagccgtgg
      241 acggtgtgga tgatatgcgg cacacccgcg cgcttggccg cgaagcggcc caagatgccc
      301 gccttggaac tgtgggtgtg taccaggtcc accttctcca ggcgcaggat ctcggtgagg
      361 cgcttgaggg ccaggaagtc ccaatagggg tggatggggt gcttgagttc ggaaaggaaa
      421 tacttgcggc agtccttgat ccagcgggcc tcctcgtcca accgcccgcc ctcgccgcag
      481 atcaggatca cctcgtattt tttgcggtcg tggtgctggc agcagtagag ggtgttctgc
      541 tgggcgccgc ccagttccag gcgggtgatg aggtgggcta ccttcaggcg tcggcgcatg
      601 gggcctccgg gacggcgttc aggcgtccgg cttgggggcc ggatccccgc cggggggcgc
      661 cttggcggcc ttggccttgc gggcggcctt cttggccgcc ttctcgtcgg ccttgaactc
      721 ggccagggcc gcgtggtcca cctcggtgat gggctgggcg gcggccttga tggcccgttc
      781 ctcgtcctcg ttctggacga agcgctgcat ccattcccac tggcgcgcca ggccttcctt
      841 cagggacacc ttgggtttga agccgaagat ccgctccaac ttggcggtgt ccgcccgggt
      901 ggagaacacg tcgcccttct ggcgctccag gaaggtgcgg gcgcaggcct tgccgctgac
      961 ctcttccagg gacgcgacgg cttccagcag ggtggcgggc gaacccccgc ccacgttcac
     1021 cacctggccc a
//
LOCUS       DASTJJ010000729         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1079061, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000729 DASTJJ010000000
VERSION     DASTJJ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079061"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1254)
                     /gene="tyrS"
                     /locus_tag="VFR02_08820"
     CDS             complement(19..1254)
                     /gene="tyrS"
                     /locus_tag="VFR02_08820"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9870582.1"
                     /translation="MTTPLSPQDQLDRILDGAAEVISKPELLERLQTAQKEGRPLKVK
                     WGADPSAPDIHLGHTVVLRKLRQFQDLGHQVQFLIGDFTAMIGDPTGKSATRKPLSRE
                     EVEANAKTYLEQVFRVLDEDPAKIEVRRNSEWCRPMAFEDVIRLASKYTVARILERDD
                     FTNRMKEQKPISLHELLYPLIQGYDSVMLRSDVEMCGTDQKFNCLVGRALQQEVGQKP
                     EVILAMPILEGLDGVKKMSKSLGNYVGVTDPPNEMFSKLMSVSDDLMWRYYQLLSARS
                     RDLEKLKAGVAGGARHPKTTKMELAEEITARFYGPEKAKEAWSYFENRHQLSGTVDYP
                     QVSVEAGGYKAADFLVRVGVSKTKSDAKRLIEQGAVDWVDAGGEKRKIASFGDPIELA
                     SGQTGVIRSGKVFLKVSAS"
BASE COUNT          234 a          476 c          434 g          295 t
ORIGIN      
        1 aaaccggccg gaaaaagcct aggacgccga gactttcagg aaaaccttcc ccgaccggat
       61 gacccccgtc tggcccgatg ccagctcgat gggatcgccg aaggaggcga tcttccgctt
      121 ttcgcccccg gcgtccaccc agtccaccgc cccctgctcg atgaggcgct tggcgtcgct
      181 cttggtcttg gacacgccca cccgcaccag gaaatcggcg gccttgtagc cccccgcctc
      241 cacggacacc tggggataat cgaccgtccc gctcaattgg tgccggttct cgaaatagct
      301 ccaggcctcc ttggcctttt cgggcccgta aaagcgcgcg gtgatctcct cggccagctc
      361 cattttggtg gtcttgggat gccgggcccc gccggccacc ccggccttca gtttttccag
      421 gtcccgggac ctcgccgaca acagttggta atagcgccac atgagatcat cggacacgct
      481 catgagcttg gagaacatct cgttgggcgg gtcggtcacg cccacgtaat tgcccaggct
      541 cttggacatc ttcttcaccc cgtcgagccc ctccaggatg ggcatggcca ggatgacctc
      601 gggtttctgc cccacctcct gctgcagggc ccggcccacc aggcagttga acttctggtc
      661 ggtgccgcac atctccacgt ccgaccggag catgaccgag tcgtaaccct ggatgagcgg
      721 atagaggagc tcgtgcaggc tgatgggttt ctgctccttc atgcggttgg tgaagtcgtc
      781 ccgttccagg atgcgggcca cggtgtattt ggaggccagg cggatgacgt cctcgaaggc
      841 catggggcgg caccattcgc tgttgcgccg cacctcgatc ttggccgggt cctcgtccag
      901 cacccggaag acctgctcca ggtaggtctt ggcgttggcc tccacctcct cccggctcag
      961 gggtttgcgg gtggccgact tgcccgtggg gtcgccgatc atggccgtga agtcgccgat
     1021 gaggaactgc acctgatgcc ccaggtcctg gaactggcgc agcttgcgca gcaccacggt
     1081 gtgccccagg tggatgtcgg gggcggaagg gtccgcgccc cacttgacct tcaaggggcg
     1141 gccctccttt tgggcggtct ggagccgctc caacagctcg ggtttggaaa tgacctcggc
     1201 ggctccgtcg aggatgcggt ccaactggtc ctggggggaa aggggcgttg tcatggccgc
     1261 catgataaca aaaacccacg ggccgggccc tcatttttgt ctgaggaatc cccgtcccag
     1321 cgcctataat ggcccttcac gtttccacca gggaggttct tcctttgccg aacgaaaaga
     1381 ccaaggcgtc cggatcccgc ttttggaaat attttttcct catcgccttc gccctggtg
//
LOCUS       DASTJJ010000730         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_224598, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000730 DASTJJ010000000
VERSION     DASTJJ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224598"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFR02_08825"
     CDS             complement(<1..634)
                     /locus_tag="VFR02_08825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9870583.1"
                     /translation="MSAPVHRPSYRQAFLFSVIGVLLGLGAPLGALALLWFGPHPELR
                     LPSFVFDEWRDHLFFFTYMLAGTSLSFGLFGFFLGRHADAIWHQNRRLTDQVLTDPLT
                     GLGNHRFLHEAFQEEAGRQASGGRPLSCLMLDLDHFKRVNDTYGHPFGDEVLRGVAAR
                     IREGLRPGDRAARYGGEEFLCLLPDCDAAAARGVAERVRAAVEAKPFQRGG"
     gene            complement(688..>1199)
                     /locus_tag="VFR02_08830"
     CDS             complement(688..>1199)
                     /locus_tag="VFR02_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HET9870584.1"
                     /translation="GLAWGYQLWLNLAAKHKMVEPKYQDLPSEKLPVVEKDGARVKVL
                     AGEYGGAKSPGVSYIPFSYFDVTLTPGARFEAPAPKDQNAFLYVFEGKAKTGAAEDPS
                     YVKTGYLGVFGPGDRVRVEAAEGAPARFLFASAQRLGEPVARGGPFVMNTVGEVKKAF
                     LDYQTGQLG"
BASE COUNT          210 a          396 c          427 g          166 t
ORIGIN      
        1 ggcccccgcg ctggaagggc ttggcctcca cggcggcccg cacccgctcg gccacgcctc
       61 gggcggccgc cgcgtcgcaa tcgggcagga ggcagaggaa ctcctcgccg ccgtagcggg
      121 cggcccggtc gccgggacgc agcccttccc ggatgcgggc cgccaccccc cgcagcacct
      181 catcgccgaa gggatggccg taggtgtcgt tgacccgctt gaaatggtcc aggtccagca
      241 tcaggcagga cagggggcgc ccgcccgagg cctggcggcc ggcctcttcc tggaaggcct
      301 catgcaggaa gcggtggttg cccaggccgg tcagggggtc ggtgagcacc tggtcggtca
      361 ggcggcggtt ctggtgccag atggcgtcgg cgtggcggcc caggaaaaaa ccgaagaggc
      421 cgaaggacag gctggtgccc gccagcatat aggtgaagaa gaaaagatgg tcccgccact
      481 cgtcgaagac gaaggaaggc aggcgcagtt cgggatgggg cccgaaccaa agcagggcca
      541 gggcccccag gggcgcgccc agccccagga ggacgccgat gaccgagaac aagaaggcct
      601 gccggtagga aggtcgatgg acgggggcgg acatggattt agtatagatc gcgccgacgg
      661 cgccgggcca aaaacccggg gcccggttca gcccaattgc ccggtttggt agtccaaaaa
      721 ggcctttttg acctcgccca cggtgttcat cacgaagggg ccgccccgcg ccacgggttc
      781 cccgagcctt tgggccgagg cgaagaggaa ccgcgccggc gcgccctccg ccgcctccac
      841 ccgcacccgg tcgccggggc cgaagacgcc caggtacccg gtcttgacgt aggaaggatc
      901 ctcggcggcg ccggtcttgg ccttgccctc gaagacatag aggaaggcgt tctggtcctt
      961 gggcgcgggg gcctcgaagc gcgcgccggg ggtcagcgtc acgtcgaaat aggaaaaagg
     1021 gatgtaggag acacccgggc ttttcgcgcc gccgtattcc ccggccagca ccttcacccg
     1081 cgcgccgtcc ttttccacga ccggcaattt ctcggacgga aggtcctggt acttgggctc
     1141 gaccatcttg tgtttggcgg ccaggttcag ccaaagctgg tagccccagg ccaggccgt
//
LOCUS       DASTJJ010000731         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1811769, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000731 DASTJJ010000000
VERSION     DASTJJ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811769"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..292
                     /locus_tag="VFR02_08835"
     CDS             113..292
                     /locus_tag="VFR02_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870585.1"
                     /translation="MTVAFRVVQNEVFPSVPGNSPVRLELRDKMGVPLANGLYYVWVS
                     VGGGRSSPAKLLVLR"
     gene            complement(289..987)
                     /locus_tag="VFR02_08840"
     CDS             complement(289..987)
                     /locus_tag="VFR02_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870586.1"
                     /translation="MAEVNPTVLVIEDEPPIRRFLRVSLENRNLKTLEAAGGEEGLAL
                     AASHNPDIILLDLGLPDLDGLVVLERLRQWASTPIIILSARGKEEDKVKALESGADDY
                     LTKPIGVEELNARIKVALRHAQRAAGGPAEPVLKSGSLKVDLEKRQVFVSAKEVHLTP
                     IQYKLLSALARQAGKLVTQSELLREIWGPSGGASSHYLRIYIHQLRHKLEENPANPQY
                     LITETGIGYRLRVD"
     gene            complement(1043..>1511)
                     /locus_tag="VFR02_08845"
     CDS             complement(1043..>1511)
                     /locus_tag="VFR02_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870587.1"
                     /translation="QPLEEVVGSALARMQKKLSGHPVRTQLPEDLPMLALDSLLMEQV
                     FINLLDNAAKYTPPGSAIEIQAQALGGWMRVEVADRGPGLSKGDEERIFDKFYRGPQS
                     GQQSGAGLGLAICRGAVEAHGGEIWAENRESGGTRFCITLPLDPEAAVVQVVE"
BASE COUNT          221 a          477 c          524 g          289 t
ORIGIN      
        1 cgtgccatat tggccccctt tgtaggccca ttctaaagcc aactgtgact ttatttctgg
       61 accaggtttt ggggggcagg tcagggggac ggcggatatt cgggtggaga tcgtgacggt
      121 ggctttccgg gtggtgcaaa acgaggtctt cccatcggtg ccgggaaaca gtccggtgag
      181 gctggagctt cgggacaaga tgggggtgcc gttggccaac ggcctttatt acgtctgggt
      241 ctcggtggga gggggccggt cgtcgccggc caagctgctg gtgctgcgct agtccacccg
      301 cagccggtaa ccgatgcccg tctcggtgat gaggtactgg gggttggcgg ggttctcctc
      361 cagcttgtgc cgcagttggt ggatgtagat gcgcaggtaa tggctggagg cgccgcccga
      421 ggggccccag atctcccgca gcagttcgga ctgggtcacc agcttgcccg cctggcgggc
      481 caaggcgctc agcaatttgt attggatggg tgtcaggtgc acttccttgg cgctgacgaa
      541 gacctgccgc ttttccaggt ccaccttcag gctgcccgac ttcaacacgg gctccgcggg
      601 cccgccggcg gccctttggg cgtggcgcag ggccaccttg atgcgggcgt tcagttcctc
      661 cacgccgatg ggcttggtca ggtagtcgtc cgcccccgat tccagcgcct tgaccttgtc
      721 ctcttccttg ccccgggccg agaggatgat gatgggcgtg gaggcccact gccgcaggcg
      781 ttccagcacc accaggccgt ccaggtcggg gagccccagg tccagcagga tgatgtccgg
      841 gttgtgggaa gcggccaggg ccaggccctc ctcgcccccg gcggcctcca gggtcttgag
      901 gttgcggttc tccagcgaca cgcgcaggaa acgccggatg gggggttcgt cctcgatcac
      961 caaaaccgtc gggttcactt cggccatgaa aagctccgtt ccttaagatg aagatgcgga
     1021 tgacgccgga atgggaccgg gctcattcga ccacctgcac gacggccgcc tcggggtcca
     1081 gcggcagggt gatgcagaaa cgggtgccgc cggactcgcg gttctccgcc cagatctccc
     1141 cgccgtgggc ctccacggcc ccccggcaga tggccagccc cagcccggcg ccgctttgct
     1201 gtccggattg ggggccccgg tagaacttgt cgaagatgcg ttcctcgtcc cccttggaaa
     1261 ggcccggccc gcggtcggcc acttccaccc gcatccagcc gcccagcgcc tgggcctgga
     1321 tctcgatggc ggacccgggg ggcgtgtatt tggccgcgtt gtccagcagg ttgatgaaga
     1381 cctgctccat caacaggctg tccagggcga gcatgggcag gtcctcgggc agttgggtgc
     1441 gcacgggatg gccggagagc ttcttctgca tgcgggccag ggccgacccc accacttcct
     1501 ccaggggctg g
//
LOCUS       DASTJJ010000732         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_833365, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000732 DASTJJ010000000
VERSION     DASTJJ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833365"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="purB"
                     /locus_tag="VFR02_08850"
     CDS             complement(<1..813)
                     /gene="purB"
                     /locus_tag="VFR02_08850"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9870588.1"
                     /translation="MIARYTLPEMGRLWSEEAKFDAWLRVELAACEAWAKLKKIPAKD
                     LAVIKKKARFSVPRIEAIERKTNHDLIAFLTSVAEFVGPSSRFIHLGLTSTDVVDTAQ
                     SLQLVQATDLLAGQLSRLRQVLKAQALKHRHSMMIGRTHGVHAEPVTFGLKMALWYDE
                     VGRHQDRLRMARHQVAVGKISGAVGTFANIDPRVEERVCRLLKLKPAPVSTQTLQRDR
                     LAFYLSVLAGIGNSLEKFATEIRNLQRTELLEAEEFFAEGQKGSSAMPHKRNP"
     gene            complement(995..>1315)
                     /locus_tag="VFR02_08855"
     CDS             complement(995..>1315)
                     /locus_tag="VFR02_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870589.1"
                     /translation="ACAEVWFLTMPYEAQAAAFCGGLAYIGWTSYLLLFGLFLTARIP
                     RKVLLFWFLFNLVVDSLLRLHVLLGARDLFSIFLLLAMPFIFCVCLTTLWVYSFDKRN
                     GPEA"
BASE COUNT          268 a          393 c          446 g          208 t
ORIGIN      
        1 cgggttgcgc ttgtgcggca tggccgagga gcccttctgg ccctcggcga agaattcctc
       61 ggcctccaac agctcggtgc gctgcaggtt gcggatctcg gtggcgaact tttccagcga
      121 attgccgatg cccgccagca cggacaggta aaaggccagg cggtcgcgct gcagggtctg
      181 ggtggagacc ggggcgggct tgagcttgag caggcggcag acccgttcct ccacccgggg
      241 gtcgatgttg gcgaaggtgc ccaccgcccc cgagatcttg cccacggcca cctggtggcg
      301 agccatccgc aggcggtcct ggtggcggcc cacctcgtcg taccaaaggg ccatcttgag
      361 gccgaaggtc acgggttcgg cgtgcacccc gtgggtgcgg ccgatcatca tggaatgacg
      421 gtgtttcaag gcctgggcct tcaggacctg gcggagccgc gacaattggc ccgccaaaag
      481 gtcggtggcc tggaccaact gcagggactg ggccgtgtcc accacgtcgg tggaggtgag
      541 ccccaggtgg atgaagcgtg aggagggacc gacgaactcg gccaccgagg tcaggaaagc
      601 gatgaggtcg tggttggtct tgcgctcgat ggcctcgatg cggggcacgc tgaaccgggc
      661 ctttttcttg atgaccgcca ggtccttggc cgggatcttc ttgagcttgg cccaggcctc
      721 gcaggcggcc agttccaccc tcagccaggc gtcgaatttc gcctcttcgg accagagccg
      781 gcccatctcg ggaagggtgt aacgcgcgat caatggatcc ccctgttcaa aaaatccccc
      841 tggaaaaaga aatcacgtcc cccggcgccg gaggaagcgg tcgatgtcga aagaaagcgt
      901 ggaatggcga gtggggaaag cgcgcacagt ataggggact tggccccctc caccaacaaa
      961 aaaacccggg atttcacggg cttttcgccc cgcctcaggc ctcaggtccg ttgcgtttgt
     1021 cgaaggaata gacccacaag gtggtcaggc acacgcagaa aatgaagggc atggcgagca
     1081 ggaggaaaat gctgaagagg tcccgggcgc ccagcagcac gtggagccgc aggaggctgt
     1141 ccaccaccag gttgaagagg aaccagaaaa gcaggacctt ccgggggatg cgcgccgtca
     1201 ggaaaagccc gaagagcaac aggtaggagg tccaaccgat ataggccagt cccccgcaga
     1261 aagccgcggc ctgcgcctca tagggcatgg tcaggaacca gacctccgca caggc
//
LOCUS       DASTJJ010000733         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_346134, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000733 DASTJJ010000000
VERSION     DASTJJ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346134"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFR02_08860"
     CDS             <1..742
                     /locus_tag="VFR02_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870590.1"
                     /translation="AIAYNPRYAKAYNNMGVVYFKQGQYEKAIEAFKKSVEIEPDYKT
                     AQNNLAVALKKRMVLTEASDALIKQIMADPDNPTLHFELGMVYKNTGQNQEAAREFQE
                     SLRLNPKHQQALEQLGETFLNLKAFDDAVKALAKAVSVNPASPEAHLQLGKVYIERGW
                     WDEALVELKKTKDLKPNFPDVHFYLGQVYRSKSWWDEALDEFQKYLDSPGDDVDKAGQ
                     ARDVMQQIETWKKQLQTSPSAGNTKLIK"
     gene            749..1087
                     /locus_tag="VFR02_08865"
     CDS             749..1087
                     /locus_tag="VFR02_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9870591.1"
                     /translation="MKLLICVISPKKLEQVKKVLWEAGVRGVTLSEASGYGYQKVQVD
                     SIRGADYKVQYQPRIRIEIALLDDEVEKVVDLLLDTVRTGRIGDGKFFLMPLDNVIRV
                     RTGEQGENAL"
     gene            1123..1485
                     /locus_tag="VFR02_08870"
     CDS             1123..1485
                     /locus_tag="VFR02_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870592.1"
                     /translation="MGQRVLLVDDASFMRMMLKNILAGAGYEIVGEAENGAKGIEMYR
                     SLKPDLVIMDIIMPEMGGIDAVREIVKANPSAKVLMCSSMGQQSLVVEAIQAGAKDFI
                     VKPFQPSNVLEAVKKTLN"
     gene            1508..2140
                     /locus_tag="VFR02_08875"
     CDS             1508..2140
                     /locus_tag="VFR02_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HET9870593.1"
                     /translation="MPALNDFQLDALREVGNVGAGNAATALSQLTGRRIDLSVSKVCV
                     LPTDAIAQFLGGSDADVASVCLPVYGDVTGVVLVFFPLDQIPSLTRMLLPHAAEPVDP
                     AHLTDIERSALREMGSILTGAYLGALFRFIRIQILHGLPELVLDMAQAILDSVLVELE
                     EKEDLAIVIESELTAQDQKMTSRFLLLPEAGSLERLFKAFANALGLPGNG"
BASE COUNT          456 a          669 c          686 g          352 t
ORIGIN      
        1 ggccatcgcc tacaaccccc gttacgccaa ggcctacaac aacatgggcg tggtctactt
       61 caagcaggga cagtatgaga aggccatcga ggccttcaag aagtccgtcg agatcgagcc
      121 ggactacaag accgcccaga acaacctggc ggtggccttg aagaaacgca tggtgctcac
      181 cgaggcctcc gacgccctca tcaaacagat catggccgac ccggacaacc cgacgctgca
      241 tttcgagctg ggcatggtct acaagaacac gggccagaac caggaggccg cccgggagtt
      301 ccaggaatcc ctgcggttga accccaaaca ccagcaggcg ctggaacagt tgggggagac
      361 cttcctcaac ctgaaggcct tcgacgacgc ggtcaaggcc ctggccaagg ccgtatcggt
      421 caatcccgcc tcgccggagg cccacctgca gttgggcaag gtctacatcg agcgcggttg
      481 gtgggacgag gccctggtgg aactgaaaaa gaccaaggac ctgaagccca acttccccga
      541 cgtgcatttc tacctggggc aggtctaccg ctccaagtcc tggtgggatg aggccctcga
      601 cgagttccaa aaatacctgg attccccggg ggacgacgtg gacaaggcgg gccaggcccg
      661 ggacgtgatg cagcagatcg agacgtggaa aaagcagctc cagacctccc ccagcgcggg
      721 gaacaccaag ctcatcaaat aggggcccat gaaactactc atttgcgtca tttcgcccaa
      781 gaagctggag caggtgaaga aggtcctgtg ggaggccggg gtgcggggcg tgaccctgtc
      841 ggaggcctcg ggctatggat atcaaaaagt ccaggtggac tcgatccggg gcgcggatta
      901 taaggtacaa taccaaccca gaatccgcat cgagatcgcc ttgctggacg acgaagtgga
      961 aaaggtcgtg gacctgttgc tggacacggt ccgcacgggc cggatcggcg acggaaaatt
     1021 cttcctcatg cccctggaca acgtcatccg ggtccggacg ggggaacagg gcgagaacgc
     1081 cctttgagag ggtgccatca tcataagtag ggggaggcgg ctgtgggtca acgagtgttg
     1141 ctggtggacg acgcgtcatt catgcggatg atgctcaaga acatcctggc gggcgcgggc
     1201 tatgagatcg tcggcgaggc cgagaacggc gccaagggca tcgagatgta caggagcctg
     1261 aaacccgacc tggtcatcat ggacatcatc atgcccgaga tgggcgggat cgacgccgtg
     1321 cgcgagatcg tgaaggccaa cccctcggcc aaggtcctga tgtgcagttc catgggccag
     1381 cagtcgctgg tggtcgaggc catccaggcc ggcgccaagg acttcatcgt gaagcccttc
     1441 caaccctcca acgtgctgga agccgtcaag aaaaccctca actaggccga gccgaccgga
     1501 gggggccttg cccgcactca acgacttcca attggacgcc ctgcgcgagg tggggaacgt
     1561 gggcgcgggg aacgcggcca ccgccctgtc ccagctcacg ggccgccgca tcgacctgtc
     1621 ggtgtccaag gtctgcgtgc tcccgacgga cgccatcgcc cagttcctcg gcgggtccga
     1681 cgcggacgtg gcctcggtct gcctgcccgt ctacggcgac gtcacgggcg tggtcctggt
     1741 cttcttcccc ctggaccaga tcccctcgct cacccgcatg ctcctgcccc acgccgccga
     1801 acccgtggat ccggcccacc tgaccgacat tgaacggtcg gccctgcgcg agatgggcag
     1861 catcctgacc ggggcctacc tgggggccct tttccgcttc atccgcatcc agatcctgca
     1921 cggcctgccc gaactggtgc tggacatggc ccaggccatc ctggacagcg tgttggtcga
     1981 gctggaggaa aaggaagact tggccatcgt gatcgagtcc gaactcacgg cccaagacca
     2041 gaaaatgacg agccgtttcc tgcttttgcc cgaggcggga agcctggaga ggcttttcaa
     2101 ggccttcgcc aacgccctgg ggcttcccgg caatggatga aaaggattgg gtggtgggga
     2161 tcg
//
LOCUS       DASTJJ010000734         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1201256, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000734 DASTJJ010000000
VERSION     DASTJJ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201256"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1371
                     /gene="mraY"
                     /locus_tag="VFR02_08880"
     CDS             283..1371
                     /gene="mraY"
                     /locus_tag="VFR02_08880"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9870594.1"
                     /translation="MLYHLFYPLADKFIGFNVFRYVTFRSVYAAITAFGLCLLLGGAL
                     IARLRAAQLGEKIRHDGPASHSAKAGTPTMGGVLILLTMLVSVLLWVRWDSKYLWLAV
                     FALAWFGLVGFADDYWKILGKGKSRGIPSTTKFMLQALGAIIIVALYLSALPDDFALR
                     TSITLPFVKVPVDLGMLYPFFAVLVIVGASNSVNLTDGLDGLAIGCSTFTAMAFVVIT
                     YVSGNVKFSEYLKIISVPGTGELTVVCAALVGAGLGFLWFNAHPAQIFMGDTGSLALG
                     GLLGTVAVMVKQEVLLIIVGGIFVVEALSVIIQVTSFKFSGKRVFKMAPLHHHFELSG
                     VPESKLIVRFWIVAIILTLLTLSTLKLR"
     gene            1499..>2539
                     /gene="murD"
                     /locus_tag="VFR02_08885"
     CDS             1499..>2539
                     /gene="murD"
                     /locus_tag="VFR02_08885"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9870595.1"
                     /translation="MIYVHNKQVAVIGLGRSGLAAARLLAVRGAQVVVIDEKTPEKLG
                     PWIDKARDLPLKLSLGRMDLAAILSSDLVVTSPGVPFDHPALEQAREQGIPVLGELEL
                     AYGYCPAPVAAITGTNGKTTTTALTAAMIQAGGKKALACGNIGRAFAEAVFELTAEDW
                     AVLEVSSFQLEAIQTFRPDVAAVLNVTPDHLDRHGSMQAYVEAKARIFENQRPEDAAV
                     LNLGDKYTPIFAGLLKSRLHLFGFADKAKDQGRPGCYVVGDRIELLGRPLIKASELRI
                     PGPHNLENACAAALMASLAGTPDAAIARALAEFPGVEHRLEKAGEVEGVTFINDSKGT
                     NVDSVVKALESFP"
BASE COUNT          422 a          797 c          856 g          464 t
ORIGIN      
        1 gcgccggggc atgcgggtgt ggcatttccc cacgacgggc ctggcgggca agttcctgcg
       61 ccggacgctg gaaaaaggcg acgcggtgct cttgaagggc tcccggggca tggccctgga
      121 acggattttg gaggagttca aggtttgaag ctgtttcacc gcaaagccgc taaggcgcca
      181 agaaaggtgc ggccgaggaa ttaggaagac ggtttgcctt tgcgtcttgg cgtctttgcg
      241 gtgaatgggt cttgatttta tcaaagaagg atcggagatc ggatgctgta ccacttattt
      301 tacccgctgg cggacaagtt catcggcttc aacgtcttcc gctacgtgac cttccgcagc
      361 gtctacgcgg ccatcacggc cttcggcctg tgcctgctcc tgggcggggc gctcatcgcc
      421 cggctgcggg ccgcccaact gggggagaag atccggcacg acgggccggc cagccactcg
      481 gccaaggcgg gcacgcccac catgggcggg gtgctgatcc tgctgaccat gctggtcagc
      541 gtgctgttgt gggtgcgttg ggactccaag tacctgtggc tggcggtctt cgcgctggcc
      601 tggttcggct tggtgggttt cgcggacgac tattggaaga tcctgggcaa gggcaagagc
      661 cggggcatcc ccagcaccac caagttcatg ctgcaggccc tgggcgccat catcatcgtg
      721 gccctctacc tgtcggccct gcccgacgac ttcgccctgc gcacctccat cacgctgccc
      781 ttcgtcaagg tgccggtgga cctgggcatg ctgtacccct tcttcgccgt gctggtcatc
      841 gtgggggcct ccaactcggt caacctcacc gacgggttgg acggcctggc catcggctgt
      901 tccaccttca cggccatggc cttcgtggtc atcacctacg tgtcgggcaa cgtgaagttc
      961 agcgaatacc tgaagatcat cagcgtgccc ggcacgggcg aactgaccgt ggtctgcgcg
     1021 gccctggtgg gggcgggctt ggggttcctc tggttcaacg cccacccggc ccagatcttc
     1081 atgggggaca ccgggtccct ggccctgggg ggcctgttgg gcacggtggc ggtcatggtc
     1141 aagcaggaag tgctgctcat catcgtgggg ggcatcttcg tggtcgaggc cctgtcggtg
     1201 atcatccagg tgacctcctt caagttcagc gggaagcggg tcttcaagat ggcccccctg
     1261 caccaccatt tcgagctctc gggcgtgccc gagtccaagc tgatcgtccg cttctggatc
     1321 gtggccatca ttttgacgct gctgacgctg tcgaccctga aactgcgctg agggaaaaca
     1381 tgttcaccac caaggcacca agacaccaag aaatgaaaaa aggataccgg aaaaaatatc
     1441 ttttttgccg tacttggtgc cttggtgcct tggtggtgaa aaaggtcagg ggttttcgat
     1501 gatctacgtg cacaacaaac aggtggccgt catcgggttg ggccgctccg ggctggccgc
     1561 ggccaggctt ttggccgtgc gcggggccca ggtggtggtc atcgacgaga agacccccga
     1621 gaagctgggg ccctggatcg acaaggcccg ggacctgccc ctcaagcttt ccctggggcg
     1681 gatggacctg gccgccatcc tgagctcgga cctggtggtg acctccccgg gcgtcccctt
     1741 cgaccatccg gcgctggagc aggcgcggga acagggcatc ccggtcctgg gcgagttgga
     1801 actggcctac ggctattgcc cggcgccggt ggcggccatc acgggaacca acggcaagac
     1861 caccaccacg gccctgacgg cggccatgat ccaggcgggg ggcaaaaagg cgctggcctg
     1921 cggcaacatc ggccgggcct tcgcggaggc ggtgttcgag ctgaccgcgg aggactgggc
     1981 cgtgctggag gtctccagtt tccagttgga ggccatccag accttccggc ccgacgtggc
     2041 ggcggtgctc aacgtgacgc ccgaccacct ggaccgccac ggcagcatgc aggcctacgt
     2101 ggaggccaag gcccgcatct tcgagaacca acggcccgag gacgccgccg tgctcaacct
     2161 gggggacaag tacaccccga tcttcgccgg attgctgaag agccggctgc acctcttcgg
     2221 cttcgccgac aaggccaagg accagggccg gcccggctgt tacgtggtgg gggaccggat
     2281 cgagctgctg gggcgcccgc tgatcaaggc ctccgagctg cgcatcccgg gcccccacaa
     2341 cctggagaac gcctgcgcgg cggccctgat ggcctccctg gccggcacgc cggacgcggc
     2401 catcgcccgg gccctggccg agttcccggg ggtggagcac cggctggaaa aggccggcga
     2461 ggtggagggc gtgaccttca tcaacgattc caagggcacc aacgtggact cggtggtgaa
     2521 ggcgctggaa agcttcccc
//
LOCUS       DASTJJ010000735         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_225107, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000735 DASTJJ010000000
VERSION     DASTJJ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225107"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..>1231
                     /locus_tag="VFR02_08890"
     CDS             67..>1231
                     /locus_tag="VFR02_08890"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET9870596.1"
                     /translation="MSRVLEQLKIARQAWAALAMATTGERNRALAAMAEGLEKEAPAV
                     LAANREDLKEARRAGLSGALLSRLELNEKKIRAMVQGIRDVEALEDPLGRSLSTVTRP
                     NGLQITKLTVPIGVIAVIYESRPNVTSDVAALAVKSGNAIVLRGGKESLRTNRAVAAV
                     LEAALGRVGLPSGCVQFIDTPDREAVQELLGATGLLDLVIPRGGESLIQAIVENSRVP
                     VIFQYKGVCHTFVDEQADLAMAEAIAVNAKTSNPAVCNAMECLLVHRGVAERFLPRLA
                     EKLTERRVEVRADGEAKAWMPGAVQAGPEDFGREFLDLVLAVKVVGSLDEALAHIDRY
                     GSRHSEAIVTNDYANARTFLARVDAACVYVNASTRFTDGGEFGFGAEVGISTQK"
BASE COUNT          210 a          390 c          442 g          189 t
ORIGIN      
        1 cgcctgatgc ggggcgagcg ggtggggacc ttcttccatc ccgcccaaag atcgtaaggg
       61 ggccgcatgt cgagggtctt ggaacaactg aagatcgccc gccaagcctg ggcggccctg
      121 gccatggcca ccacggggga acggaaccgg gccctggccg ccatggccga agggttggaa
      181 aaggaagccc cggccgtcct ggcggccaac cgggaggacc tgaaggaggc ccgccgggcc
      241 ggcctctcgg gggccctgtt gtcccgcctg gaactgaacg agaaaaaaat aagggccatg
      301 gtccagggga tccgggacgt ggaggctttg gaggacccct tgggccggtc cctttccacc
      361 gtgacccgcc ccaacggcct gcagatcacc aaattgaccg tgcccatcgg ggtgatcgcg
      421 gtcatctacg aatcccggcc caacgtcacc tcggacgtgg cggccctggc cgtcaagtcg
      481 ggcaacgcca tcgtgcttcg gggcggcaag gaatccttgc ggaccaaccg ggcggtcgcc
      541 gcggtgttgg aggcggcgct gggccgggtg gggctgccct ccggatgcgt gcagttcatc
      601 gacacccccg atcgggaggc cgtccaggag ctgctgggcg ccacgggcct gctggacctg
      661 gtcatccccc ggggcgggga atccttgatc caggcgatcg tggaaaactc ccgggtcccg
      721 gtcatcttcc aatacaaggg ggtctgccac accttcgtgg acgaacaggc cgacctggcg
      781 atggccgagg ccatcgcggt gaacgccaag acctccaatc cggcggtctg caacgccatg
      841 gaatgcctgt tggtgcaccg gggcgtggcg gaacggttcc tgccccgctt ggccgaaaaa
      901 ctgaccgagc gccgggtgga agtgcgggcg gacggggagg cgaaagcctg gatgcccggg
      961 gcggtgcagg ccggcccgga ggattttggc agggaattcc tcgacctggt gctggctgtc
     1021 aaggtggtgg ggtccctgga cgaggccctg gcccatatcg accgctatgg gtcgcgccat
     1081 tcggaggcca tcgtgaccaa cgattacgcc aacgccagga cattcctggc ccgggtggac
     1141 gcggcctgcg tctacgtcaa cgcttccacg cgtttcaccg acgggggcga gttcggtttc
     1201 ggggcggagg tcggcatcag cacgcagaaa c
//
LOCUS       DASTJJ010000736         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_468502, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000736 DASTJJ010000000
VERSION     DASTJJ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468502"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..180)
                     /locus_tag="VFR02_08895"
     tRNA            complement(106..180)
                     /locus_tag="VFR02_08895"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Glu,seq:ctc)
     gene            complement(288..1679)
                     /locus_tag="VFR02_08900"
     CDS             complement(288..1679)
                     /locus_tag="VFR02_08900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9870597.1"
                     /translation="MILFWGTSVPAFANPPALPQTPPLSIPAPAFDVKTLPCGLKVIF
                     LKDDSLPLVSCDLMIPGGAAAEPAGQEGVASLLAALLRDGGAGKLAPEAFDEALEDRG
                     ASMSAGADTEGFSAGFKCLAQDLPDVMGLFADMLRRPAFDVGRLETDRAQLVDGLDRT
                     QDVPDDLTRTLFYRALYGGHPYGRTATPAEAAKLTRDEVTAFYRSQFGPRGAVLALSG
                     DFDEAKALARLGSLFGDWKPQAAPAAYAEPKPLGPKVYFYPKQVGQVFIRLGYLGLKR
                     HDPDDVPLQVANYILGGSGFASRLMAHIRSDRGLAYFVETFFQPKDIRGPFELVGGTR
                     PDAVKEFLTLCFQMLGDYAAAGPTQAEVDAAEKSMVEEYAYNFESPFSLASYKASLDF
                     HGYPDDYLATYRQKVKSVTREQASQAIRKVLDQPDWVLVVCGPEALQKDLEGFGKVVE
                     VSDIYAPWPLDKP"
     gene            complement(1814..>2897)
                     /locus_tag="VFR02_08905"
     CDS             complement(1814..>2897)
                     /locus_tag="VFR02_08905"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9870598.1"
                     /translation="HQVDVNNELSNIYTLNGGQSLNAWTSQDCTAYTVTLPANKVPVY
                     ARIEKDRISHPVFREFYTERDVVAQERRWRTESNPEGRLYEALQSTAYSAAPYRDPAI
                     GWMTDIQKLTRPDAVAFYRQTYRPDRGVLAVVGGIKAADVLPLLERTLGTVPNPSVPP
                     LKPDWTVEPPQTGPREVHTTFDSDPMAMMAWHVPNFPRPDSVALDVLCSILTSGNTSR
                     LVKDLVFTRKMVHSVEASTGFPGDRDPNLLVIEFEPSPGYSSKQVTGAVEADLADLKA
                     KGPTPAELDRAKEGAEAGFLWGKTSTMGLAEDLAYNQAVHGSWTYTQTYLDRVRALTP
                     ADIQRVAQKYLLENNRTIAYLERASQ"
BASE COUNT          501 a          875 c          986 g          535 t
ORIGIN      
        1 gcccggaaac cgatggattt tgaatctaaa agttaatttt ggttagatca ggggtagatc
       61 agaaaaaaag aaagcccgga atcctaggat tccgggcacg attattggta gccccacggg
      121 gaatcgaacc ccgatctaat ggctgagaac catccgtcct gaccgttaga cgatggggcc
      181 gctgaaaaac cagttttggg tttttagtgc tgagttctta gtcgacccag caggaagagg
      241 attttaagag aagctccccc gctttacaac ctgaaaaacc ggcgtcttta gggcttatcc
      301 agcggccagg gggcgtaaat gtccgaaact tccaccacct tgccgaaacc ctccaggtcc
      361 ttctgaaggg cctcgggccc gcagaccacc agcacccaat caggctggtc gaggaccttc
      421 cggatcgcct gggaggcctg ttcccgggtg acggacttca ccttttgacg gtaggtggcc
      481 aggtaatcat ccgggtagcc gtggaagtcc agcgaggcct tgtaggaggc caggctgaag
      541 ggcgattcga agttgtaggc gtattcctcg accatggact tttccgccgc gtccacctcg
      601 gcctgggtcg gcccggcggc ggcgtagtcg cccaacattt ggaagcagag ggtcaagaat
      661 tccttcacgg cgtcgggccg ggtgccgccc accagttcga aggggccccg gatgtccttg
      721 ggctggaaga aggtctccac gaagtaggcc aatccccggt cggaccggat atgggccatc
      781 aggcgggagg cgaaccccga tccgcccagg atgtagttgg ccacctgcag gggcacgtcg
      841 tcgggatcat gccgcttcag gcccaggtac cccagccgga tgaagacctg gccgacctgc
      901 ttgggataaa aatagacctt gggccccagg ggcttgggtt cggcgtaggc cgcgggggcc
      961 gcctggggtt tccaatcgcc gaagagcgat cccaaccgcg ccagggcctt cgcctcgtcg
     1021 aagtcgccgg aaagcgccag cacggcgccc cggggcccga actgggaccg gtagaaggcc
     1081 gtcacctcgt cccgggtcag cttggccgct tccgcgggcg tggccgtgcg gccgtagggg
     1141 tggccgccat agagcgcgcg gtaaaaaagg gtgcgggtca ggtcgtcggg cacgtcctgg
     1201 gtgcggtcga gcccgtccac caactgggcc cggtcggtct ccaaccgtcc gacgtcgaag
     1261 gccggccgcc gcagcatgtc ggcgaagagg cccatcacgt cgggcaggtc ctgcgccagg
     1321 catttgaagc ccgcgctgaa gccctcggtg tcggccccgg cgctcatgga ggcgccccgg
     1381 tcctccagag cctcgtcaaa ggcctccggc gcgagcttcc ccgccccgcc gtcgcgcaac
     1441 aaggccgcca acagggacgc cacgccttcc tggccggcgg gctcggcggc ggcgcccccc
     1501 gggatcatca ggtcacagga caccagcggc aggctgtcat ccttcaggaa gatcaccttc
     1561 aacccgcaag gaagggtctt cacgtcgaag gccggggccg ggatggacaa gggcggcgtt
     1621 tggggcaacg ccggtgggtt ggcgaaggcc gggaccgacg ttccccaaaa aaggatcatc
     1681 aaagttctta attttaagtt ttgagttctt ggttttttgg ggaccaaagc cacgggcttg
     1741 gaacttggac cgggaattcc tcgctccttg gccccactcg gcgccttggc gtcttggcgg
     1801 tggaatggtc ttttcattgg gacgcccttt ccaggtaggc gatggtccgg ttgttttcca
     1861 gcaggtattt ttgggccacg cgttggatgt ccgccggggt cagggcccgg acgcggtcca
     1921 gataggtttg ggtgtaggtc cagctgccgt gcacggcctg gttataggcc aggtcctcgg
     1981 ccaggcccat ggtggaggtc ttcccccaga ggaatccggc ttccgcgcct tccttggcgc
     2041 ggtcaagctc ggccggcgtg ggtcccttgg ccttcaggtc cgccaggtcc gcctcaacgg
     2101 cccccgtcac ctgtttggag gaatagcccg gcgagggctc gaattcgatg accagcaggt
     2161 tcgggtcgcg gtcgccgggg aatccggtgg aggcttccac cgaatggacc atcttccggg
     2221 tgaagaccag gtccttcacc aggcgggagg tgttgcccga ggtcaggatg gagcaaagca
     2281 cgtccagggc caccgaatcg ggcctgggga aattaggcac atgccaggcc atcatggcca
     2341 tggggtccga atcgaaggtg gtgtgcactt cccgggggcc cgtctggggc ggctcgacgg
     2401 tccaatcggg cttcaggggg ggcacggacg ggttgggcac ggtgcccagg gtgcgttcca
     2461 agagcggtag cacgtcggcg gccttgatcc cgcccaccac cgccagcacg ccgcggtcgg
     2521 gccggtaggt ctgccggtaa aaggccacgg cgtcgggccg ggtcagtttt tggatgtccg
     2581 tcatccaacc gatggccggg tcccggtagg gcgcggccga ataggcggtg gattgcaggg
     2641 cctcgtagag gcgcccctcg gggttggatt cggtgcgcca gcgcctctcc tgggccacca
     2701 cgtcccgttc ggtatagaac tcccggaaga ccgggtggct gatgcggtcc ttctcgatcc
     2761 gcgcgtagac cggcaccttg ttggccggca gggtgaccgt ataggccgtg cagtcctggc
     2821 tggtccaggc gttcaggctc tggcccccgt tgagggtgta gatattggaa agctcgttgt
     2881 tgacgtcgac ctggtgt
//
LOCUS       DASTJJ010000737         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_101350, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000737 DASTJJ010000000
VERSION     DASTJJ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101350"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..>1024
                     /locus_tag="VFR02_08910"
     CDS             63..>1024
                     /locus_tag="VFR02_08910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9870599.1"
                     /translation="MSSETASPFNPRILVVGRLSGPKNRVILNYLEAAASAVSGEFPG
                     ASFQVLGGPVGEEHRALEARLPSVRFLGHQQALGDFYEQADLVVGSGRVALEAMARKK
                     PVVAVGERLYLGPLTEENRPRVLQSNFGDCAAEEGFDWGRAAQDLLTLLKDPAKREQA
                     ARTGWSLLKTEYNMDVVYPQLENLYQDAVLDQNLASRHELPVLMYHRLAQRAPETSRF
                     NLHVTAADFEDQLAWLKGWGFTPVTFGDLAQKRAPRKPVILTFDDGYEDNYQNLFPLL
                     KKHGVRAVVYLLGDRSIRRNEWDIPKGEPEAALLNDAQVREMAQ"
BASE COUNT          182 a          339 c          342 g          161 t
ORIGIN      
        1 ctgcgaggac atccgcgacc agatgctcac ccaaacccgc gtgttccaac cggagaggct
       61 ccgtgtcatc cgaaacggca tcccccttta acccccggat cctggtggtg gggcgcctgt
      121 ccgggcccaa gaaccgggtg atcctcaact atttggaggc cgccgcgtcc gcggtttccg
      181 gggaattccc cggggcctcc ttccaggtgt tgggcgggcc cgtgggcgag gagcaccgcg
      241 ccttggaggc gcggctgccc tcggtgcggt tcctgggcca ccagcaggcc ctgggggatt
      301 tctacgagca ggcggacctg gtggtgggat cgggccgggt ggccctggag gccatggcga
      361 ggaaaaaacc cgtggtggcg gtgggggagc gcctgtacct ggggcccctg accgaagaga
      421 accggccccg ggtgcttcag agcaatttcg gcgattgcgc cgccgaagaa gggttcgact
      481 ggggccgggc ggcgcaggac ctcttgaccc tgctgaagga cccggccaag cgggaacagg
      541 ccgcccggac cggttggtcc ttgcttaaaa ccgagtacaa tatggacgtg gtctaccccc
      601 aactggaaaa cctctaccag gacgccgtcc tggaccaaaa cctcgcctcc cggcatgagc
      661 tgcccgtcct gatgtaccat cgtctggcgc agcgggcgcc ggaaacctcc cgcttcaacc
      721 tgcacgtgac cgcggcggac ttcgaggacc aactggcgtg gctgaagggg tggggcttca
      781 cgcccgtcac cttcggcgac ctggcgcaaa aacgcgcgcc ccgcaagccc gtcatcctca
      841 cctttgacga cggctatgag gacaactacc agaacctctt cccgttgttg aagaagcacg
      901 gcgtccgggc ggtggtgtat ctgctggggg accggtccat ccgccgcaac gagtgggaca
      961 tccccaaggg cgaacccgag gccgcgctcc tcaacgacgc ccaagtgcgg gagatggccc
     1021 aaag
//
LOCUS       DASTJJ010000738         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_834188, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000738 DASTJJ010000000
VERSION     DASTJJ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834188"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFR02_08915"
     CDS             <1..243
                     /locus_tag="VFR02_08915"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HET9870600.1"
                     /translation="HGADTVEEKLSLADRFGLVVSFYAPDQDTFFKMVDEWAKVEGIK
                     MPAADLHLKAAQWARFNNGRSGRAARQFINDLKGKQ"
     gene            314..1156
                     /locus_tag="VFR02_08920"
     CDS             314..1156
                     /locus_tag="VFR02_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9870601.1"
                     /translation="MASAHPAPSPFPERLLLEGKQIQMVSGAGKVEWTYEAPGPFLDA
                     QPQPSGDWLVTGGPGKVFLLHHTHGKRPWEPGWDWSDLDTDPPVGAVAVDWDLDGRPT
                     LVLAADAVKQRVFLAEAKGARAKVRWEFALPEAPRSVRVCPDSGNFLVTLPSQVAELD
                     FKAGKISWSLPAEGAQDAARGPDSLTYVVDSRGKVQAFDAGKRSQWAADLDPQASDWS
                     GLSLSLFEVRQGVRLLLSGTARTRHGRQERVWVLDAQDGHVLAEQPEDASVTRDVPWI
                     EGME"
     gene            1153..1806
                     /locus_tag="VFR02_08925"
     CDS             1153..1806
                     /locus_tag="VFR02_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870602.1"
                     /translation="MTGASRKKAKKPSDGFPLCLNHPSRKVLGFCHVCGDRLWWECLE
                     EGRQYYYCRKPKCQRAWRESLLSECPSCGKPVRGKPIFCRACGYRLEPISAEETDDLV
                     TVARFGTSLEAQLAQTKLTAERIASYVGDEHVVSLMPLYDLPWGGVKLRVRHKDLARA
                     LQALGEGKAGSFWTPRLLYAGTYVTGHVVVGFLMMVVGVLLFFICRYFGWWCFGEWR"
     gene            1803..>2059
                     /locus_tag="VFR02_08930"
     CDS             1803..>2059
                     /locus_tag="VFR02_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870603.1"
                     /translation="MRRAWSLAALLGAPLLLAAPPLSAQTTGPNGLRLDFQTGIQVEG
                     APVDYFQAARVLLDRDESLVDGLDLRWGGEALWMAARGNPLP"
BASE COUNT          335 a          627 c          700 g          397 t
ORIGIN      
        1 cacggggccg acacggtgga ggaaaaactg tccctggccg accgcttcgg cctggtggtc
       61 tccttttacg cgcccgacca ggacaccttc ttcaagatgg tggacgaatg ggccaaggtc
      121 gagggcatca agatgcccgc cgccgacctg cacctgaagg ccgcccaatg ggcccgcttc
      181 aacaacggcc gctcgggccg cgccgcccgg cagttcatca acgacctgaa gggtaaacaa
      241 tagcttgaaa aaacactttc cgggtgtcct gggcgcactt ggcggtctga ccgccgttct
      301 ggttttgggc ccgatggcct cggcccatcc ggccccgtcc ccctttcccg aacggctgct
      361 tttggaaggc aaacagatcc agatggtctc gggcgcggga aaggtggaat ggacctatga
      421 ggcgcccggc cccttcctgg acgcccaacc ccagccttcg ggcgactggc tggtgaccgg
      481 cggcccgggc aaggtctttt tgctccacca tacccacggg aaaaggccct gggagcccgg
      541 ctgggactgg tcggacctcg acacggaccc gccggtgggc gcggtggcgg tggactggga
      601 cctggacggc cgtcccacgc tggtgctggc cgccgacgcg gtgaagcagc gcgtcttttt
      661 ggcggaggcc aagggcgccc gcgccaaggt ccgctgggaa ttcgccctgc ccgaggcgcc
      721 ccggtcggtg agggtctgcc cggacagcgg caatttcctg gtgaccctgc cttcccaggt
      781 ggcggagctc gacttcaagg cgggcaagat ctcctggagc cttcccgccg agggggccca
      841 ggacgcggcc cgaggcccgg acagcctcac ttatgtggtc gactcccggg ggaaggtgca
      901 ggccttcgac gcgggtaagc gatcccaatg ggcggcggac ctggaccccc aggcctcgga
      961 ctggtccggc ctgtccctct cgctcttcga ggtccggcaa ggggtccgat tgctcctctc
     1021 gggcacggcc cgcacccgcc acggccggca ggagcgggtt tgggtgctcg atgcccagga
     1081 cggccatgtg ctggccgagc aaccagagga cgcctccgtc acgcgtgatg taccgtggat
     1141 cgaggggatg gaatgacggg ggcgtcccga aaaaaagcca agaagccttc cgatggtttt
     1201 ccgctttgtc tgaaccatcc cagccgcaag gtgctaggtt tttgccacgt ttgtggagac
     1261 cggctttggt gggagtgcct ggaagagggt cggcaatatt attattgccg taaacccaaa
     1321 tgccaaagag cttggcgcga aagcctgctt tcggagtgcc cttcctgtgg aaagccggtg
     1381 aggggaaagc ctattttttg ccgcgcttgc ggttaccgac tggaaccgat ttcggccgaa
     1441 gaaaccgacg atttggtgac ggtggcgcgc ttcggcactt ccttggaagc ccaattggcg
     1501 caaaccaagc tcactgccga gcgaatcgcg tcttacgtgg gggatgagca tgtggtttct
     1561 ctcatgcctt tgtatgacct tccgtggggc ggagtgaagc tgagggtccg ccacaaggac
     1621 ttggcccgag cccttcaggc tttgggggag gggaaagccg gcagtttttg gaccccgcgt
     1681 ttgttatatg ccggaaccta cgtgacgggg cacgtggtgg ttgggttttt aatgatggtt
     1741 gttggggtgc ttttattttt catttgtcga tactttggat ggtggtgttt tggggaatgg
     1801 cgatgaggcg cgcctggtcc ctggccgcgc tcctgggggc gcccctgctc ctggcggcac
     1861 cgcccctttc ggcccagacc accgggccca acggacttag gctcgatttc cagaccggca
     1921 tccaggtgga aggcgcgccg gtggattatt tccaggccgc ccgggtgttg ctggaccggg
     1981 acgaatccct cgtggacggc ctggacctgc gctggggcgg ggaagccctc tggatggcgg
     2041 cccggggcaa ccccctgcc
//
LOCUS       DASTJJ010000739         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1691308, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000739 DASTJJ010000000
VERSION     DASTJJ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691308"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFR02_08935"
     CDS             complement(<1..526)
                     /locus_tag="VFR02_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9870604.1"
                     /translation="MDTSSDPGFDLGGIIEGGHLIPYFQPIVSVKNNSIVGYEGLVRG
                     LVPGSAQTIPPLDLFAEARSQGLTLALDRLCRKQIFQMFKTIQARRPERLLSVNFEAS
                     VLDQGVQGSGHIIQQVREAGLNPQSIIIEVIESNVEEVEALERFIATYREMGFLIALD
                     DVGAGHSNLNRIPLL"
     gene            945..>1910
                     /locus_tag="VFR02_08940"
     CDS             945..>1910
                     /locus_tag="VFR02_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9870605.1"
                     /translation="METELAAPKEKKPMSETDLTALIEQKSVAAFYQPIISIKKKSVV
                     GIESVGRGIDTGNRRLIEPGELYPISFEKNKALDLDRLFRLKGLEGYAEIQAKIPGLI
                     LFLNLESAVLTDEVVGSGHLLKMVRQLGLNPSLITIELSQSAGMDLTAVKRFSEIHRE
                     SGFLIGLEDITANRTCLDQILHIAPDVIKINPSVVDGVSKDPYKREDFKTILNLSRKI
                     GCLVVADGVENEEDALVALELGADMLQGSYFSKPQRTENSSILGLKARILFVASRYKR
                     ILTDKINKDKDRRTRYLAIVSEIFDSLGGGGDYERRFQEFLTRYPQ"
BASE COUNT          414 a          611 c          543 g          342 t
ORIGIN      
        1 tgagcagggg gatccggttc aggttcgaat ggcccgcccc cacgtcgtcc agggcgatga
       61 ggaaccccat ctcccggtag gtggcgatga agcgctccag cgcctccacc tcctccacgt
      121 tggactcaat gacctcgatg atgatgctct gcgggttgag cccggcctcc cgcacctgct
      181 ggatgatgtg gcccgagccc tgcacgccct ggtccagcac ggacgcctcg aaattgaccg
      241 acagcagccg ttcgggacgc cgggcctgga tggtcttgaa catctggaag atctgcttgc
      301 ggcacaggcg gtccaacgcc agggtgaggc cctgggaccg ggcctcggcg aaaaggtcca
      361 ggggcgggat ggtctgggcg gaacccggca ccaatccccg gaccaggccc tcgtagccca
      421 cgatggaatt gttcttgacc gaaacgatgg gttggaaata agggatgaga tgtccgccct
      481 caatgatgcc gcccaggtca aaacccggat ccgacgatgt gtccattggc gtgccttttc
      541 ccaagaaagg gtgctccctt ttttaagcag acgcatcccg actgtggcaa caactgtgcc
      601 attcgggatt ttccctggaa tccgggcccg tccgcgcttt ccccgcgcca aaagcctcaa
      661 caaaagtctg catttggggg gacttccgac acttttgtag gcgcgccaca gcatttcaaa
      721 caaaccaagc ggcttcaagt taaatgccgg caaaaagatc ggaacccctc gaaaatccgc
      781 cgagaacttt atggatgggc cctgaacaaa aagcttattc caggcccctt tccccaaagg
      841 aggatcccca cctttgggtc gatgaggtaa aggaacaacc ctcttatttt aagccactgg
      901 ttggcacatc ccttgcttta ccccatccca aaacaggagg acccatggaa accgaactcg
      961 ccgccccaaa ggaaaagaaa cccatgtccg agaccgacct gaccgccttg atcgagcaaa
     1021 aaagcgtcgc cgccttctat caacccatca tttccatcaa gaagaaatcc gtggtcggca
     1081 tcgagtccgt gggacggggc atcgacaccg gcaaccgccg gctgatcgaa cccggggaac
     1141 tctaccccat ctccttcgag aagaacaagg ccttggacct ggaccgcctg ttccggctca
     1201 agggattgga aggttatgcc gagatccagg ccaagatccc ggggctcatc ctctttctca
     1261 acctcgagtc ggccgtcctc accgacgagg tggtggggtc cggccacctg ctcaagatgg
     1321 tgcgccagct gggcctgaac cccagcctga tcaccatcga gttgtcccag tccgcgggca
     1381 tggacctgac cgcggtcaag cgcttctccg agatccaccg ggaaagcggg ttcctcatcg
     1441 gcctggagga catcacggcc aaccgcacct gcctggacca gatcctccac atcgcccccg
     1501 acgtcatcaa gatcaacccc tcggtggtgg acggcgtctc caaggacccc tacaagcggg
     1561 aggacttcaa gaccatcctg aacctgagcc gcaagatcgg ctgcctggtg gtggccgacg
     1621 gggtggagaa cgaggaggac gccctggtgg ccctggagct gggcgccgac atgctgcagg
     1681 ggagctattt ttccaagccc cagcgcacgg aaaattccag catcctgggg ttgaaggccc
     1741 gcatcttgtt cgtggcctcg cgctataagc gcatcctgac ggacaagatc aacaaggaca
     1801 aggaccggcg cacccgctac ctggccatcg tctccgagat cttcgacagc ctgggcggcg
     1861 ggggcgatta cgagcggcgg ttccaggaat tcctgacgcg ttacccccag
//
LOCUS       DASTJJ010000740         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_959466, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000740 DASTJJ010000000
VERSION     DASTJJ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959466"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..>1247)
                     /locus_tag="VFR02_08945"
     CDS             complement(152..>1247)
                     /locus_tag="VFR02_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9870606.1"
                     /translation="GQRGWTLIELMIVTALIGIITPAMTYLFMRMSQGMAADEMRLQL
                     QKMNTNTTLRIRDRLLANKHLYQNDAFGPSFVAQLKFGATAPPTLAGSLLPVIQTNTS
                     LAPGYGAATAVGNSLLLAVNDDTATMSGTIYYAPLTVQGTNITWSTPVIPAPTPQPTA
                     VPATITIDVYRFYYYYLTATNPRPIPNVNTYRLVEWQSVPFADFHEISAIPDPALMKQ
                     VVSWIASANSVTTGSAAITLGYDPSASAVTNAFFRLTSGGVTAAITSPSQQILQAGWT
                     YMTHASSGILSSGFGYGIAPNSPPWDYVPTVPQYGTASGNFPSGFEVAVDGPEEGRQV
                     LMRILAVGKGGSPLAIYNDMTTVNPVRDSW"
BASE COUNT          196 a          344 c          472 g          235 t
ORIGIN      
        1 cacccgcgcg ccttccaggg gcggcaggcc gccctcgcag ggtttgcaat ggcgggaagc
       61 gggggctcgg gggtcttcca tgggggtcct tagcaggccc ggacgggcct tcgggtcatt
      121 ggggaaaatc tctggtccgc ggagggccgc gttaccagct gtcccgaacg ggattgacgg
      181 tggtcatgtc gttgtagatg gccaaagggc ttccgccttt gcccaccgcc aagatccgca
      241 tgagcacctg gcgtccctcc tcgggcccgt ccaccgccac ctcgaacccc gaggggaaat
      301 tccccgaggc ggtcccgtat tggggcacgg tgggcacgta gtcccacggc gggctgttgg
      361 gggcgatgcc gtagccgaag ccgctgctca ggatgcccga ggaggcgtgg gtcatatagg
      421 tccagccggc ctgtaggatc tgttggctgg gcgacgtgat ggcggcggtc accccgcccg
      481 acgtcaggcg gaaaaaggcg ttggtgaccg cgctggcgct ggggtcgtac cccagggtga
      541 tggccgcgct gcccgtggtc acgctgttcg cgctggcgat ccaggacacc acttgcttca
      601 tcagggcggg atccgggatg gccgatatct cgtggaaatc agcgaagggg acgctctgcc
      661 attccaccag ccggtaggtg ttcacattgg ggatgggacg ggggttggtg gcggtgaggt
      721 aatagtaata gaaccggtac acgtcgatgg tgatggtggc aggcacggcg gtgggttggg
      781 gtgtgggcgc ggggatgacc ggcgtgctcc aggtgatgtt ggtgccctgg acggtcaggg
      841 gggcgtaata gatggtgccg ctcatcgtgg cggtgtcgtc gttgacggcc aagagcaagc
      901 tgttgcccac cgccgtggcc gcaccatatc cgggggccaa ggacgtgttg gtctggatga
      961 cgggcaacaa ggaccccgcc agggtggggg gggccgtggc gccgaacttg agctgggcca
     1021 cgaaggaagg cccgaaggca tcgttctggt acaggtgttt gttggccagc aatcggtccc
     1081 ggatgcgcag ggtggtgttg gtgttcatct tttggagctg cagccgcatt tcatccgcgg
     1141 ccatgccttg gctcatgcgc atgaacaggt aggtcatggc gggcgtgatg atgccgatga
     1201 gcgccgtgac gatcatcaac tcgatgaggg tccagcccct ttggccg
//
LOCUS       DASTJJ010000741         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_834496, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000741 DASTJJ010000000
VERSION     DASTJJ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834496"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFR02_08950"
     CDS             complement(<1..325)
                     /locus_tag="VFR02_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870607.1"
                     /translation="MKGHLGEVVEGAAAAFLLRALGAALAFGFNWLVARRYGAGGAGI
                     FFLALTVLAFATVLGKMGLDNSLLRFIAANAAVRHWKAVKGVYARGMTLALLVSGAIS
                     LLLFLA"
     gene            complement(437..>1509)
                     /locus_tag="VFR02_08955"
     CDS             complement(437..>1509)
                     /locus_tag="VFR02_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870608.1"
                     /translation="QGASATGVIWMATLGAILSFLLFNWRPAKVYLGDTGSSALGGLA
                     AILLVGLGSPGPSLFYFLQPDQPVPEAVRFHFVAATLLAGYPALEVTLSAFRRGAKFL
                     YFGRSMEWSEQEHIHHRLLKLGIKPGAICLTACLCQVLFSSAGVLIMVKQNALATWLM
                     VPFLMGFAYAAPRLGIVRFLGMDQSVDKPYYRIAHNFIAFQRSKLALARSREQILALV
                     SQTCREFGVRCFRFKIQPDAGGKGGMDYLFRVPETRMDARDLHLMDNPADGEKGWYFD
                     YYEIPGRRDEAFWLFDHHAGDDELDVEYRVLMSGFMREALQFAVILGEGLETLDLPSV
                     MTLPHDEMSGHRLRQRDRSKFN"
BASE COUNT          350 a          495 c          408 g          256 t
ORIGIN      
        1 ccgccaggaa gagtaggaga gaaatcgccc cggaaaccag gagcgccagc gtcatcccgc
       61 gggcatagac gcccttcacc gccttccagt ggcggaccgc cgcgttggcc gcgatgaacc
      121 gcagcaggct gttatccaac cccatcttcc ccagaaccgt ggcgaatgcc aggaccgtca
      181 aggccaagaa gaaaatgccc gcgccacccg cgccgtatcg tcgggcgacc aaccaattga
      241 acccaaaagc cagggcggcg cccaaagccc tcaatagaaa agcggctgcg gccccctcga
      301 ccacttcccc cagatgtccc ttcatttttc ggcgcaggaa tgggattctt ttcaggattg
      361 aacggaacga atttttcatc gagctccgat gtccattgta ttcttccggc tatgggaggc
      421 ccggcccctc ctggtcctaa ttgaacttgg accggtccct ttgacgcagg cggtgtccgc
      481 tcatctcatc atgcggcaag gtcatgacgc taggcaggtc cagggtctcc aacccttccc
      541 ccaggatgac cgcgaactgc agcgcttccc gcatgaaccc gctcatcagc acccggtatt
      601 ccacgtccaa ttcatcgtcc cccgcatggt ggtcaaaaag ccaaaaagcc tcgtcacgcc
      661 ttccggggat ctcgtaataa tcgaagtacc aacccttctc gccgtcggcg ggattgtcca
      721 tcaaatgcag gtcccgagcg tccatccggg tttccgggac gcggaaaaga taatccatgc
      781 ctcccttgcc cccggcgtcg ggttggatct tgaaccggaa acaccgcacc ccgaattccc
      841 ggcaggtctg gctcaccagg gccagaatct gttcgcggct ccgagccaag gccaacttgg
      901 atcgttgaaa agcgatgaaa ttgtgggcga tacggtaata gggtttgtcc acggattggt
      961 ccatccctag gaaccgcacg atgcccagcc ggggcgccgc ataggcgaac cccatcaaga
     1021 agggcaccat caaccaggtc gccagggcgt tctgtttcac catgatcagg acacccgcgc
     1081 ttgaaaaaag cacctgacat aggcaggcgg tcagacaaat ggcgccgggc ttgatgccca
     1141 gcttgagcag tcgatgatgg atatgttcct gttcggacca ttccatcgac ctgccaaaat
     1201 agaggaactt ggcccctcgt cggaaggccg agagggtcac ctccagggca ggatatccgg
     1261 ccaaaagggt ggccgcgacg aaatggaacc gcaccgcctc cggtaccggc tgatccggtt
     1321 gcaaaaaata aaaaagggac ggcccgggcg aacccaggcc caccaacagg atggccgcca
     1381 aaccacccag ggcgctcgag cccgtatccc ccagatagac cttggccggc cgccaattga
     1441 ataaaagaaa ggacaggatc gcccccaaag tcgccatcca gatcaccccg gtggccgagg
     1501 ccccctgca
//
LOCUS       DASTJJ010000742         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_590298, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000742 DASTJJ010000000
VERSION     DASTJJ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590298"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1551
                     /locus_tag="VFR02_08960"
     CDS             166..1551
                     /locus_tag="VFR02_08960"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HET9870609.1"
                     /translation="MFLSIRELAQESQLSEHLAFDALGEVVTPGVIEAVIGQTDAREE
                     RVRKLPASVMLVFCIAMNLLAAESLSSVFARLVRGLKWSGIHPNAVQASKSALCQARY
                     RLGERPLIALFHQVCRPLATPTTKGAFIFGLLHVAIDAQTLDLPDTAANERHFGRPTT
                     ARGLCAFPQAYLVGLLECATHAFFDATLVPYPASLHQAAQRLVRSITHGMLVSFDCGL
                     HSYPLIRAIRTKGAHVLSRLSAGVRPQIVKELHDGSVLARIFPSSGIQRRQRALLVRM
                     ITYTFDDPNRPGYGEVHRLLTTLLDPTLAPAKELIVAYHNRWEYELAVDEVETHLRPR
                     LPLRSKLPAGIIQEVYGLLIAHYLVRKVMHDAAVQADLPPIRLSFLETVRLLRDYLPD
                     FQRHAPQEQPFLYQALLDEVKAAKLPPRANRINPRVVKRKMSNFNVKRPCHKHWPQPT
                     KAFQEAIVLLI"
BASE COUNT          285 a          584 c          418 g          298 t
ORIGIN      
        1 tggccgtcat cggcctggtg acgatcccgc gggatcgggc ggaggaggtc aaggattggg
       61 ggctaggggt cgggggttag gtccaatacg gttcagataa gacgcaaaca gctcctgtag
      121 actaaagggg ccattaccca cccttcggtc aacaggagct gtctgatgtt tttgagtata
      181 cgggaactgg cccaagaaag ccaactgagc gagcacctgg cgttcgacgc cctgggcgag
      241 gtggtgaccc cgggagtgat tgaggcggtg atcggacaaa ccgacgcgcg cgaggaacgg
      301 gttcgcaaac tccccgcgtc ggtgatgctg gtcttctgca tcgcgatgaa cctcctcgcc
      361 gccgaatcgc tcagctccgt ctttgcccgc ctcgtgcggg gcttgaaatg gtcgggaatc
      421 caccccaacg ccgtgcaggc cagcaaaagc gccctctgtc aggcacgcta tcgcttaggg
      481 gagcgccccc tgatcgcctt gttccaccag gtttgccgtc ccctggccac ccccacgacc
      541 aaaggcgcct tcatctttgg gttgctccac gtggcgatcg atgcccaaac cctcgacctg
      601 cccgataccg cggccaatga acggcacttc ggccgtccca ccaccgcccg agggctctgt
      661 gccttccccc aggcgtacct ggtcgggttg ctcgaatgcg ccacccacgc cttcttcgac
      721 gccaccctcg tcccctaccc cgccagcctc catcaggcgg cccagcgtct ggtccgctcg
      781 atcacgcacg gcatgctcgt gagcttcgat tgcggcttgc acagctaccc cttgatccgg
      841 gcgatccgaa ccaaaggcgc ccacgtcctc tcgcgcttgt ccgcgggtgt tcgcccgcag
      901 attgtcaagg aactgcacga tgggagtgtc ctcgcccgga tcttcccctc gtcgggcatc
      961 cagcgccgac aacgcgctct gctggtgcgc atgatcacct acaccttcga tgatcccaac
     1021 cgccccggct acggcgaggt ccatcgcctc ctgaccaccc tcctcgaccc gacccttgcc
     1081 ccggccaagg aactcatcgt ggcctaccat aatcgctggg aatacgaact cgccgttgac
     1141 gaggtcgaga cccatctgcg tccccgtctc cccttgcgct cgaaactccc cgccggcatc
     1201 atccaggagg tctacggcct cttgatcgct cactacctcg tgcgcaaggt catgcacgac
     1261 gccgcggtgc aggccgatct tcccccgatc cgcctcagct tcctggagac cgtgcgcctc
     1321 ctccgcgatt accttcccga cttccagcgt catgccccac aggagcagcc ctttctctac
     1381 caggccctcc tcgacgaggt caaagctgcc aaacttcccc cgcgcgccaa tcgcatcaac
     1441 ccgcgggtcg tcaaacgcaa gatgtccaac tttaacgtga aacgcccctg ccacaaacac
     1501 tggccccaac ccaccaaggc gtttcaggag gccattgtcc tccttatcta aaccgtattg
     1561 gacctaaccc ccagaccctt ccctc
//
LOCUS       DASTJJ010000743         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_834961, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000743 DASTJJ010000000
VERSION     DASTJJ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834961"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFR02_08965"
     CDS             <1..943
                     /locus_tag="VFR02_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET9870610.1"
                     /translation="GRIKIKVLGKEIDLRVSICPVQFGEKIVMRILDSSNLSLDLPKL
                     GFEEDVLPKFQKAIVEPYGLVLVTGPTGSGKSTTLYSALHYINDPDTNISTIEDPVEY
                     NLPGINQVNAKAGIGLTFAAGLRSFLRQDPDVIMVGEIRDKETGEIAINAALTGHLVF
                     STLHTNDAPGAVTRMGNMGIEPFLITSTVHCVVAQRLLRRICKDCKQSYEATPEVLEE
                     MGLQPTGEPVTLYKGAGCATCSNTGYKGRMAIHEVLVLNDDFRKAVLQRKPASELKKV
                     ARLGGMQTLRECGVQKVLKGMTSVEELFRVAHADEEE"
     gene            974..>1648
                     /locus_tag="VFR02_08970"
     CDS             974..>1648
                     /locus_tag="VFR02_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9870611.1"
                     /translation="MVNIDTLLRLMFERGASDLHITAGAPPMLRVDGVMTATEYEKLR
                     PETTQQLIYSILTDEQKEKFEKDNELDVSFGVEGVGRVRMNVFQQRGAVASALRNIPT
                     TIRSFEDLKLPQVVQDLVKLPKGLVLVTGPTGSGKSTTLAAMLDWINSNQQHHIITIE
                     DPIEYVHTHKSCIVNQREVGTDTHSFTAALKYALRQDPDVILIGEMRDLETINAALTI
                     AETGHLV"
BASE COUNT          349 a          525 c          492 g          282 t
ORIGIN      
        1 cggccgcatc aagatcaagg tattgggcaa ggagatcgac ctgcgcgttt ccatctgccc
       61 cgtccagttc ggcgagaaga tcgtcatgcg catcttggac tcctccaacc tgtcgttgga
      121 cctgcccaag ctggggttcg aggaggacgt gcttcccaag ttccaaaagg ccatcgtgga
      181 accctacggc ctggtgctgg tgaccggccc cacggggtcc ggcaaatcca ccaccctcta
      241 ttcggcgctc cactacatca acgacccgga caccaacatc tccacgatcg aggacccggt
      301 ggaatacaat ctgcccggca tcaaccaggt caacgccaag gcgggcatcg ggctcacttt
      361 cgcggccggg cttcgctcct tcctccggca ggacccggac gtcatcatgg tgggcgagat
      421 ccgcgacaag gaaacgggtg agatcgccat caacgcggcc ttgaccggtc acttggtctt
      481 ttccacgctt cacaccaatg acgcgcccgg cgcggtcacc cgcatgggca atatgggcat
      541 cgaacccttc ctgatcactt cgaccgtgca ttgcgtggtg gcccagcgcc tgttgaggcg
      601 catctgcaag gattgcaaac agtcctacga ggcgaccccc gaggtgctgg aggaaatggg
      661 cctgcagccc accggggagc ccgtcaccct ctataagggg gcgggttgcg ccacctgttc
      721 caacaccggc tacaagggac gcatggccat ccatgaggtc ctggtcctga acgacgattt
      781 ccggaaggcc gtcctgcaac gcaagcccgc ctccgaactg aaaaaagtgg cccgcttggg
      841 gggtatgcag accctgcggg aatgcggcgt gcagaaggtt ctgaagggaa tgacttccgt
      901 ggaagagctt ttccgcgtgg cacacgcgga cgaggaagaa taatccgaag ggcgacaggg
      961 acaaggggat ccaatggtca atatcgatac tttgctacgc ttgatgttcg aacggggggc
     1021 ctcggacttg cacatcacgg cgggcgcgcc tcccatgctg cgcgtggacg gcgtgatgac
     1081 ggccaccgag tacgaaaagc tccggccgga gaccacccag caactcatct acagcatcct
     1141 gaccgatgag cagaaggaaa agttcgagaa ggacaacgaa ctggacgtgt ccttcggcgt
     1201 ggagggggtg ggccgcgtcc gcatgaacgt cttccagcag cgcggggccg tggcgtccgc
     1261 cctgcgcaac atcccgacca ccatccggtc ctttgaggac ctgaaactgc cccaggtggt
     1321 gcaggacctg gtcaagctcc ccaagggcct ggtgctggtg accggtccca cggggtcggg
     1381 caagtccacc accctggcgg ccatgttgga ctggatcaac tccaaccagc agcaccatat
     1441 catcaccatc gaagacccca tcgagtatgt ccacacccat aagtcctgta tcgtcaacca
     1501 gcgggaagtg gggacggaca cccacagctt caccgcggcc ttgaagtacg ccttgcggca
     1561 ggacccggat gtcatcctga tcggtgaaat gcgcgacctg gagaccatca acgcggcctt
     1621 gaccatcgcc gagaccggcc acttggtc
//
LOCUS       DASTJJ010000744         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1447798, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000744 DASTJJ010000000
VERSION     DASTJJ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447798"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFR02_08975"
     CDS             <1..589
                     /locus_tag="VFR02_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9870612.1"
                     /translation="KLKEKGFSVVHLDPFLLREVEDGFLQVGKAAGALDAARKLSKDF
                     TAGIQALVEKVPLGAYRPKLYCEELAKPPLVPGRWWPDLMTKVGAHYFPILSREMNRQ
                     ARIEEVIKFDPDVIIFSVYGSGKDFNPDEVLKRIGWEKIMAVRKRKVYSLDPALLNWP
                     TPALLTGAQAVQSALGESYWGWPLVDPGLVRRVTN"
     gene            594..1400
                     /locus_tag="VFR02_08980"
     CDS             594..1400
                     /locus_tag="VFR02_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9870613.1"
                     /translation="MAPPRRILSLAPSNTEILFALGLGDSLVGVTEYCRHPPEARLKP
                     ALRGWVSIPPEKILELDPDLAVTSGLCQEALRRRLEAAGVKLLHLDPRDLAGVRESFL
                     ELGRATGREAQARELAERFDAEGTAVRRDAQGVRLRLYCEEWQKPPSVSGNWVPELAY
                     AAGADYFPLPPGEPSRPLRPGEIQAFDPQIVILSICGMGLKPDPSEILGRPGWEDLEA
                     LREKRVVTLDDSLLNCPGPRLWEGARALQEVLQAYRMGEVPPASTGRRNL"
     gene            1528..>2222
                     /locus_tag="VFR02_08985"
     CDS             1528..>2222
                     /locus_tag="VFR02_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870614.1"
                     /translation="MDVDAPETEERHEDLRLSLTHISQKAISSNLNDLSQVLDLAQGF
                     EQWTHRASVVYGSWIEDYRIQAQQLVVDLQTGRPVAKCMEHLLRSLLQLHRALDKERE
                     QQRAAEAVADPPPPPAPAAGLDALPVQRPFSPMPLVAPPAPEASAPAPGPASEEAGRK
                     YQESLQRTFENKINHMENLLEALGELTLCQAQLAEDLHASALSESAGLNSEAVRMEKL
                     TRQIRDLVLALRLV"
BASE COUNT          378 a          774 c          708 g          362 t
ORIGIN      
        1 aaagctgaaa gaaaagggct tttcggtggt ccacctggac cccttcctgt tgcgggaggt
       61 ggaggacggc ttcctgcagg tgggcaaggc cgcgggcgcc ctggacgccg cccgcaagct
      121 ttccaaggac ttcaccgccg gcatccaggc cctggtcgag aaggtccccc tgggcgccta
      181 ccggcccaag ctctactgcg aagaactggc caagccgccc ctggtgccgg gccgctggtg
      241 gcccgacctg atgaccaagg tgggcgccca ctatttcccc atcctgtccc gggagatgaa
      301 ccggcaggcc aggatcgagg aagtgatcaa gttcgacccc gacgtcatca ttttctcggt
      361 ctacggctcg ggcaaggact tcaaccccga cgaggtcctc aagcgcatcg gctgggaaaa
      421 gatcatggcg gtgcgcaaac gcaaggtcta cagcctggac cccgcgctcc tcaactggcc
      481 cacgcccgcc ctgctgacgg gcgcccaggc cgtgcagtcc gccctgggcg agtcctactg
      541 gggctggccg ctggtggacc cgggcctggt gcgccgggtg accaactagg cccatggcgc
      601 cgccccggcg catcctgtcg ctggcccctt ccaacaccga gatcctcttc gccttagggc
      661 tgggcgattc cctggtgggc gtgaccgaat attgccgcca tccccccgaa gcgcgcctga
      721 agcccgccct gcggggttgg gtctccatcc cgcccgagaa aattttggaa ctggaccccg
      781 acctggccgt gaccagcggc ctctgccagg aggccctgcg ccgaaggctc gaggccgcgg
      841 gcgtgaagct gctgcacctg gatccgcggg acctggccgg ggtgcgggag tccttcctcg
      901 agttggggcg ggcgacgggc cgggaagcgc aagcgcgcga gctggccgag cgcttcgacg
      961 ccgaggggac ggcggtgcgt cgggacgccc aaggcgtccg gctccggctt tattgcgagg
     1021 agtggcagaa gccgccctcg gtctcgggca attgggtgcc cgagttggcc tatgccgcgg
     1081 gcgcggacta tttcccgctg ccgcccggcg agcccagccg tcccttgcgg ccgggcgaga
     1141 tccaggcctt tgaccctcaa atcgttatcc tgtccatctg cggcatgggg ctgaaacccg
     1201 atccatcgga aatattgggc aggcccggtt gggaagatct ggaggccctg cgggaaaaaa
     1261 gggtggtcac cttggatgat tccctcctca attgtcccgg gccccgcctg tgggaggggg
     1321 cccgcgccct acaagaggtc ctgcaagcct accggatggg ggaggtcccc ccggcttcca
     1381 ccggtcgtcg aaatctttga gccggggcat ttaggcctgg attcgccaat ttgcggtcct
     1441 gtatcatgcc ccctaaatca cgctgttggc ccccggttct gacaaaatca cgacacaaac
     1501 cgtccggcgg accgaaggac tttgtctatg gatgttgacg cccccgaaac cgaagagcgc
     1561 cacgaagacc tgcgcctttc cctgacccac atcagccaaa aagccatcag ttccaacctc
     1621 aacgaccttt cccaggtcct ggacctcgcc caaggcttcg agcaatggac ccaccgggcc
     1681 agcgtggtgt acggatcctg gatcgaggac taccgcatcc aggcccagca gttggtggtg
     1741 gacctgcaga cgggccgccc ggtggccaaa tgcatggaac acctgctgcg gtccctgctg
     1801 caactgcacc gggccctgga caaggaacgg gaacaacaac gtgccgccga ggccgtggcc
     1861 gatccgccgc cgccccccgc cccggccgcc gggctggacg ccctgccggt ccaacgtcct
     1921 ttttcgccca tgccgttggt ggcgccgccg gcccccgaag cctccgcccc cgccccgggg
     1981 cccgcgtccg aggaggccgg ccgcaagtac caggaatcgc tccaacgcac cttcgagaac
     2041 aagatcaacc acatggagaa cctgctggag gctttgggcg aactgaccct ctgccaggcc
     2101 caactggccg aggacctgca cgcttccgcc ctgtccgagt ccgcgggcct caattcggaa
     2161 gccgtgcgca tggaaaaact gacccgccag atccgcgacc tggtgttggc cctgcggttg
     2221 gt
//
LOCUS       DASTJJ010000745         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1570727, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000745 DASTJJ010000000
VERSION     DASTJJ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570727"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFR02_08990"
     CDS             <1..866
                     /locus_tag="VFR02_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9870615.1"
                     /translation="FIFINLGRELPNAFSLMSSTSWDEKSQWVMAQLQSLDLGKFLQA
                     LGIGPDQAQAIVQRGISAGVQDFGQMVLAKVDFLFKHLAQIGLELGFTLVAMFFFFRD
                     GSRYALKTVELLPLDPVYQRKVAHTFSATVTAVMRAMFISAFVQGLLTGAGLALTGVP
                     LPFLFGVVAFINSFIPFLGAASVWIPGAVWLFVQDQPLPGLLLLAWGVLISIFDHIFR
                     PWLIGSEAKLPVFGLFFTIIGGLLVYGFLGIFLGPIILSMGMAFLSIYREVYLDFKAS
                     GGNKRPARARR"
     gene            1075..>1284
                     /locus_tag="VFR02_08995"
     CDS             1075..>1284
                     /locus_tag="VFR02_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9870616.1"
                     /translation="MRYRWLGQTGLNVSEIGFGAWAIGGAWWGPQDDADSRLALHQAL
                     DLGVNFIDTAWVYGDGHSEKLIGSVL"
BASE COUNT          182 a          415 c          404 g          283 t
ORIGIN      
        1 tcttcatctt catcaatttg ggccgggagc tgcccaacgc cttttcgctg atgtcctcca
       61 cctcctggga cgagaagagc caatgggtca tggcccaact gcagtccctg gacctgggca
      121 agttcctgca ggccctgggg atcggcccgg accaggccca agcgatcgtt caacggggga
      181 tctcggcggg ggtccaggat ttcggccaga tggtgttggc caaggtggac ttcctcttca
      241 agcatctggc ccagatcggg ttggagctgg ggttcaccct ggtggccatg ttctttttct
      301 tccgggacgg ttcccgctac gccttgaaga ccgtggagtt gctgccgctg gacccggtgt
      361 accaacgcaa ggtggcccac accttctcgg ccaccgtgac ggccgtcatg cgcgccatgt
      421 tcatcagcgc cttcgtgcag ggcctcttga cgggcgccgg cttggccctg accggggtgc
      481 ccctgccttt cctgttcggc gtggtggcct tcatcaattc cttcatcccc ttcctggggg
      541 ccgcctcggt ctggatcccg ggcgcggtct ggcttttcgt gcaggaccag cccctacccg
      601 gcctgttgtt gctggcctgg ggcgtcttga tctcgatctt cgaccacatc ttccgtccct
      661 ggctcatcgg cagcgaggcc aagctgccgg tcttcgggct gttcttcacc atcatcggcg
      721 ggcttttggt ctacggtttc ctggggatct tcctgggccc catcatcctg tccatgggca
      781 tggccttcct ttccatctac cgggaggtct acctggactt caaggcttcc gggggcaaca
      841 agcgccccgc ccgcgcccgc cgttagggtt gcattccggc ggggcgccgc cccacaatga
      901 agtgggcagg atccaaggcc gggaccttcc accgcaaagc cgccaaggcg tgaagggaaa
      961 aggaccccat tcctcccggt ctttggccgg agttttcact ttgcgccttg gcgtccttgc
     1021 ggtcagaagg tcttgacggg catttcaagg acattttccg gatcggagat gaccatgcgt
     1081 taccgttggc tgggccagac cgggttgaac gtgagcgaga tcggcttcgg cgcctgggcc
     1141 atcggcggcg cctggtgggg cccccaggac gacgcggatt cccggctggc cctgcaccag
     1201 gcgctggact tgggcgtgaa cttcatcgac accgcctggg tctacggcga cggccacagc
     1261 gagaagctca ttgggagcgt gctg
//
LOCUS       DASTJJ010000746         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1692310, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000746 DASTJJ010000000
VERSION     DASTJJ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692310"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..943
                     /gene="hisA"
                     /locus_tag="VFR02_09000"
     CDS             173..943
                     /gene="hisA"
                     /locus_tag="VFR02_09000"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET9870617.1"
                     /translation="MYVIPAIDIRKGKAVRLVQGDLRDETVYSQEPVSVAKLWKMKGA
                     KRLHVIDLDGAFSGKLSNLEYVKEIIKATDLKVQVGGGIRDLKTIDKLVGIGVNWVIL
                     GTTAVQNKDFVREAVKRHGKSIIVGIDAKKGMVAIKGWKQVTKVRAVDLGAEMVELGV
                     GSIIFTDIQRDGMLEGPNFKSTKELAVAVKVPVIASGGMSGYKDIEHAKRLEKYGIAS
                     VIIGKALYTGKIDLKQAIKIAKEPPAPKPPKTKKTKGR"
     gene            943..1713
                     /gene="hisF"
                     /locus_tag="VFR02_09005"
     CDS             943..1713
                     /gene="hisF"
                     /locus_tag="VFR02_09005"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET9870618.1"
                     /translation="MLSKRIIPCLDVKEGRVVKGVRFENLKDAGDPVEIAKQYDAEGA
                     DELVFLDITASSDKRAILLDVVTRTAEAVFMPLTVGGGVRTTDDIRILLKSGADKVSL
                     NTRAVEHPQVLKEGAERFGAQCIVLAIDAKRRDPSDASRGWEVFTHGGRTPTGKDAVE
                     WARQGVGLGAGEILLTSMDKDGTKDGYDLELTRAVSEAVPVPVIASGGVGTLDHLAAG
                     VTEGKADAVLAASIFHYREYSVKQAKAYLAQKGIPIRL"
BASE COUNT          387 a          556 c          612 g          292 t
ORIGIN      
        1 gaagctttga acctattcac cgcaaagacg cgaaggcgcc aaggaaagct ttggtaaaaa
       61 acaagggata tggcggcggg atccattcct gattttctta tgtccggtaa ttctttgcgg
      121 cttggcggct ttgcggtgga acaggttctg aattttttgg taaggaaaaa ccatgtacgt
      181 cattccggcg atcgacatcc gaaagggcaa ggccgtgcgc ctggtccagg gcgacctcag
      241 ggacgagacg gtctattccc aggagcccgt gtccgtggcc aagctgtgga agatgaaggg
      301 ggccaagcgc ctgcacgtca tcgacctgga cggggccttc tcgggcaaat tgtccaacct
      361 ggaatacgtg aaggagatca tcaaggccac cgacctgaag gtgcaggtgg gcggcggcat
      421 ccgcgacctc aagaccatcg acaagctggt gggcatcggg gtgaactggg tcatcctggg
      481 caccacggcg gtgcagaaca aggatttcgt ccgggaggcg gtcaaacgcc acggcaagtc
      541 catcatcgtg ggcatcgacg ccaagaaggg catggtggcc atcaaaggct ggaagcaggt
      601 caccaaggtg cgggccgtgg acctgggcgc cgagatggtg gagctgggcg tggggtccat
      661 catcttcacc gacatccagc gcgacggcat gctggagggc cccaacttca agagcaccaa
      721 ggaactggcc gtggcggtca aggtgcccgt catcgcctcg ggcggcatgt cgggatacaa
      781 ggacatcgag cacgccaagc ggctggaaaa atacggcatc gcctcggtca tcatcggcaa
      841 ggccctctat acgggcaaga tcgacctgaa acaggccatc aagatcgcca aggaaccccc
      901 cgcgcccaag ccgcccaaga ccaaaaagac gaagggacgc tgatgctctc caagcgcatc
      961 atcccctgcc tggacgtgaa ggaaggccgg gtggtcaagg gcgtccggtt cgagaacctg
     1021 aaggacgccg gcgatcccgt cgagatcgcc aaacagtacg acgccgaggg ggccgacgag
     1081 ctggtcttcc tcgacatcac ggcctccagc gacaagcgcg ccattttgct cgacgtggtc
     1141 acccgcacgg cggaggccgt cttcatgccc ctgacggtgg gcgggggcgt gcgcaccacg
     1201 gatgacatcc gcatcctgct caagtcgggc gccgacaagg tgtccctgaa cacccgggcc
     1261 gtggagcacc cccaggtgct gaaggaaggc gccgaacggt tcggggccca atgcatcgtg
     1321 ttggccatcg acgccaagcg ccgggacccc tcggacgcct cgcgcggatg ggaggtcttc
     1381 acccacggcg gccgcacccc cacgggtaag gacgcggtgg agtgggctcg ccagggggtc
     1441 gggctgggcg cgggggagat cctcttgacc agcatggaca aggacggcac caaggacggg
     1501 tatgacctgg agctgacccg ggccgtgtcg gaggccgtcc ccgtccccgt catcgcctcg
     1561 ggcggcgtgg gcaccctgga ccacctggcg gccggcgtga ccgagggcaa ggccgacgcg
     1621 gtcttggcgg cctccatctt ccattaccgc gagtattcgg tgaagcaggc gaaggcctac
     1681 ctggcccaaa aaggcatccc catccggctt tgagccggga ccatcctccg gaggacgaca
     1741 tggaccaacc cgagaacaag aaagactccc ccctgtggtt cctctatatc tatggggggt
     1801 tcggcacggt gatcggcctc ttttacctgg gcgagcatgg caaagtg
//
LOCUS       DASTJJ010000747         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_227097, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000747 DASTJJ010000000
VERSION     DASTJJ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227097"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFR02_09010"
     CDS             complement(<1..532)
                     /locus_tag="VFR02_09010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9870619.1"
                     /translation="MLIPARNEEKRLSPCLESLLAQDYPAFEVRVLDDRSTDGTWKLA
                     QGFARKHKRLRVLRGRELPAGWLGKPWACFQLAGAARGEWLFFTDADTWHAPDMLKRT
                     VQMAEARKADVLTLLTRQVTRSWMEALVIPVMAFHLLSFFPAKWSLRPRSRLNAFAGV
                     SGQFVFIRKQAYRAFGG"
     gene            660..>1152
                     /locus_tag="VFR02_09015"
     CDS             660..>1152
                     /locus_tag="VFR02_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870620.1"
                     /translation="MHLLSGVEQDFHRPQTSPGGYEWWHFDGRDQHSGFSFTAQFYAG
                     NPFSPYYQQALRRYLRESRSPLVKAPTLAPPQPLDFCGVAFRLFKHGAPAGEFFQEFT
                     PGQLKASEEQAAVLLGPNRFHWDASGDPPGYHLSLQGAVGRGRTLRARFFFIPEPLPA
                     LPAE"
BASE COUNT          188 a          402 c          379 g          183 t
ORIGIN      
        1 ggcccccgaa ggcccggtag gcttgctttc ggatgaagac gaactggccg ctcacccccg
       61 cgaaggcgtt gaggcgcgag cggggcctga gcgaccactt ggcggggaaa aaggagagca
      121 gatgaaaggc catgaccggg atgaccagcg cctccatcca actgcgcgtc acttggcggg
      181 tgaggagcgt gaggacgtcg gccttccggg cctcggccat ttgcaccgtg cgcttgagca
      241 tgtcgggggc gtgccaggtg tcggcgtcgg tgaagaagag ccactcgccc cgggcggccc
      301 ccgccagttg gaagcaggcc cagggcttgc ccagccaccc ggccggcagt tccctgcccc
      361 gcaacaccct caggcgcttg tgcttgcggg cgaagccttg ggccaacttc caggtcccgt
      421 cggtggaacg gtcgtccagc acccgcacct cgaaggcggg atagtcctgg gccaaaaggc
      481 tttccaggca gggggaaagg cgcttttcct cgttgcgggc ggggatgagc accgacacct
      541 tcggccagcg gcggcggctt gggtccggca ggggcggccg ggcgaataaa aggatgtttt
      601 cgaaaacgca gtaggttatc gtaaccgcga ggaaacccca aaggcaggcg atcaggacca
      661 tgcaccttct ctcaggcgtc gagcaggatt tccaccggcc ccagacatcc cccgggggct
      721 acgaatggtg gcacttcgac ggccgggacc agcatagcgg tttcagcttc accgcgcaat
      781 tttacgcggg caaccccttt tccccctatt accaacaggc gctgcgccgc tacctgcggg
      841 agtcccggtc gcccctggtg aaggcgccca ccctggcccc gccccagccg ctcgatttct
      901 gcggcgtggc cttccggctc tttaagcacg gcgccccggc cggggaattc ttccaggagt
      961 tcacccccgg ccagctcaag gcctccgagg aacaggcggc ggtgctcttg ggccccaacc
     1021 gcttccactg ggacgcctcg ggcgatcccc cgggttacca cctgtccctg cagggggccg
     1081 tcggccgggg ccggaccctg cgggcccggt tcttcttcat ccccgagccc ctccccgccc
     1141 tgcccgccga at
//
LOCUS       DASTJJ010000748         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_713613, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000748 DASTJJ010000000
VERSION     DASTJJ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713613"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFR02_09020"
     CDS             complement(<1..139)
                     /locus_tag="VFR02_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HET9870621.1"
                     /translation="MRTIGLLFLSNIFMTFAWYGHLKTLRDKPLMVAILVSWGIAFFE
                     YL"
     gene            282..1274
                     /locus_tag="VFR02_09025"
     CDS             282..1274
                     /locus_tag="VFR02_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870622.1"
                     /translation="MPLSRAQTTASILLVLALGFPPGLAAKPRTPKVVVNTQALVLHD
                     EARGRDIPVKVYDPRASNFPGRLPVIVFSPESGSSKDSYAYLGHYWAEHGYLCLFLAH
                     KGSDTFLLKKGKPLTSLRNIVESTRKDQNLIDRPRDISFVIDSLAEIEAQVSDLKRRV
                     DPTRVGVAGHSFGATAALAVAGATLHLSDGSARDFRDNRVTCLVAMSPPGDSSPGFDA
                     ESWRGVPRPLLILRGSQDHGLNGEPVRWRGEAFERVPPHHKYEVVLSGAGHMDFSDRQ
                     LDGSRGNSRFHAYVAQATLAFWDAFLRGNQSVQQKLDAGDFPSSRGIKAAVQVK"
     gene            complement(1287..>1816)
                     /locus_tag="VFR02_09030"
     CDS             complement(1287..>1816)
                     /locus_tag="VFR02_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870623.1"
                     /translation="WTVMGWVLARRRGRAPGHFGGWITQTPRLALVFLVSLLGLAAFP
                     GLGLFGGFYLVLLSVYRASPVLGLAAVGAFVISAWPLALLFKKIFLGPPPAGTPARVP
                     DLGVREALVFLPLFLASVWIGVGPNQFLQPMEKSVQLNVLQRLNALPAMMDFAAYQRM
                     LQDERQKAGKPPAGP"
BASE COUNT          345 a          651 c          556 g          264 t
ORIGIN      
        1 acaagtactc gaagaaggcg atgccccaac ttaccaggat ggccaccatc agcggcttgt
       61 cccgcagggt cttcaagtgc ccataccagg cgaaggtcat gaagatgttc gaaaggaaca
      121 gcagcccgat ggtgcgcata ccagcctcct agcaataggt taaacgacga tctttcctaa
      181 cgaaaaccgc cacgggaatc aaccgagccg ccaaagttcc tgtacccggg cccggtggga
      241 tgggggataa tgggccgtca cccttccccg gaggaacccg cttgcccctt tcccgcgcgc
      301 aaaccacggc ctcgatcctg ctggtcctgg ccctgggctt tccgccggga ctggccgcca
      361 agccccgtac ccccaaggtg gtggtgaaca cccaggccct ggtgctgcac gacgaggccc
      421 gcggccgcga catccccgtg aaggtctatg acccccgcgc ctccaatttc cccggccggc
      481 ttcccgtcat cgtcttctcg cccgaatccg ggagctccaa ggattcctac gcctacctgg
      541 gccattactg ggccgagcac ggttacctct gcctcttcct ggcccacaaa ggcagcgaca
      601 ccttcctgtt gaagaagggc aaacccctca ccagcctgcg caacatcgtg gaatcgacgc
      661 gcaaggacca gaacctcatc gaccgccccc gggacatctc cttcgtcatc gattccctgg
      721 ccgagatcga ggcccaagtg tccgacctga aaaggcgcgt ggatcccacc cgggtgggcg
      781 tggcggggca ttccttcggg gcgaccgccg ccctggcggt ggcgggcgcc acgctccacc
      841 tgtccgacgg atccgcgcgg gatttccggg acaaccgggt gacctgcctg gtggccatgt
      901 ccccgcccgg ggattcttcg ccgggcttcg acgccgaatc ctggcgcggc gtgccgcggc
      961 cgctgctcat cctgcggggc agccaggacc acggcctcaa cggggaaccg gtccgttggc
     1021 gcggggaggc gttcgaacgc gtgcctcccc accacaaata cgaggtcgtg ctgtcgggcg
     1081 cgggccacat ggatttcagc gaccgtcaat tggacggcag ccgcggcaac tcgcggttcc
     1141 acgcctacgt ggcccaggcc accctggcct tttgggacgc cttcctgagg ggcaaccagt
     1201 ccgtccaaca aaaactggac gcgggcgatt tcccctcgtc ccggggcatc aaggccgccg
     1261 tccaggtcaa gtgacccggc gggccgtcaa gggcccgccg gcggcttccc cgccttttgc
     1321 cgctcgtcct gaagcatgcg ctggtaggcc gcgaagtcca tcatggcggg aagggcgttg
     1381 agccgctgca acacgttcag ctggaccgac ttttccatgg gctgcaggaa ttggttgggg
     1441 ccgacgccga tccagaccga agccaggaag agcggcagga acaccagggc ttccctcacc
     1501 cccaggtcgg gcacgcgggc cggagtcccg gcgggcggcg gcccgaggaa gatcttcttg
     1561 aacaggagcg ccaggggcca ggccgagatc acgaaagcgc ccaccgccgc caggcccagc
     1621 acaggcgacg cccgatacac cgagagcaac accaggtaga aaccgccgaa caaccccaaa
     1681 cccgggaaag ccgccaaacc caagagggag accaggaaca ccagggccag ccggggggtc
     1741 tgggtgatcc aaccgccgaa atgtcccggc gcccggcccc ggcgccgcgc caacacccat
     1801 cccatcaccg tccaga
//
LOCUS       DASTJJ010000749         1455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1082019, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000749 DASTJJ010000000
VERSION     DASTJJ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082019"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFR02_09035"
     CDS             complement(<1..350)
                     /locus_tag="VFR02_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870624.1"
                     /translation="MSTLSVPNPAIFPEAPSVGGPVPSFAQKKIQLGKRDVLACFRAL
                     VLLVLAVLFFFNDRGFGQAGVDPKAFGLMTLYLASIFGMFLIKPGWFEKEVVLPGFFL
                     LDTVFITAGIYLTGV"
     gene            complement(458..>1455)
                     /gene="tgt"
                     /locus_tag="VFR02_09040"
     CDS             complement(458..>1455)
                     /gene="tgt"
                     /locus_tag="VFR02_09040"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9870625.1"
                     /translation="LACGSEILLANTYHLMLRPGADLLEKMGGLHRFMHWDKPILTDS
                     GGFQVFSLADLRKITERGVEFQSHLDGSRRELTPEDATRLQHQIGSDILMAFDECIPY
                     PSPEDYVEQSVDRTTRWAERCWRTHRESGREDRQSLYGIVQGAHFPHLREKSAKALLA
                     FDFPGYAIGGVSVGEPKEGIYRVVEQTAPLLPAEKPRYLMGVGTPEDLWECVDRGMDL
                     FDCVMPTRIARNGTALTRRGRVILKNAQYAQDERPLDGDCSCECCRHYSRAYLRHLFQ
                     AEELLVLRLVSLHNLRFMTEICEIIRAAIREGRFREAKAAFLGDYFGSVPAPGRG"
BASE COUNT          298 a          457 c          449 g          251 t
ORIGIN      
        1 acgccggtca ggtaaatacc cgccgtgatg aagaccgtgt ccagcaggaa aaagcccggc
       61 aggacgacct ctttctcgaa ccaccccggt ttgatcagga acatcccgaa aatggaggcc
      121 aggtaaaggg tcatcaagcc gaaggccttt gggtcgaccc cggcttggcc gaagccccgg
      181 tcattgaaaa agaagaggac cgcgagcacc agaaggacca gggcgcggaa acaggccaat
      241 acgtcccgct tgcccaattg gatctttttt tgggcgaatg atgggaccgg ccccccgacc
      301 gagggggcct ccgggaagat cgcgggattg gggaccgaaa gggtgctcat gccctcatga
      361 aaagcaaatg gcgtgccaaa agaaccggtg gaaaaccagg cgaaggggcc gatggaatac
      421 gtgccggctg tatccttttc gcttaggtgg gaaattttta cccgcggccc ggcgccggaa
      481 cggatccaaa gtagtccccc agaaaggccg ctttggcctc gcggaaccgg ccttcccgga
      541 tggcggcccg gatgatctcg caaatttccg tcatgaacct caaattatgc aggctcacca
      601 acctcaagac caaaagctcc tccgcctgga acaaatgccg taaataggcc cggctgtaat
      661 ggcggcaaca ttcgcaggag cagtccccgt ccaagggccg ctcatcctgg gcatattggg
      721 cgttcttgag gatgacccgg ccccgccggg tcagggcggt cccgtttcgg gcgatgcggg
      781 tgggcatcac gcagtcgaac aagtccatgc cccggtccac gcattcccag aggtcctcgg
      841 gggtgcccac ccccatcaga taccggggtt tttccgcggg caggagcggc gcggtctgtt
      901 ccaccacccg gtagatcccc tccttgggtt cccccaccga cacgccgccg atggcgtaac
      961 cgggaaaatc gaaggccagc agggccttgg cgctcttttc ccgcaagtgg ggaaaatggg
     1021 cgccctgcac gatcccatag agggactgtc ggtcctcccg cccgctctcc cggtgggtcc
     1081 gccagcaacg ctcggcccaa cgggtggtcc gatccaccga ttgctccacg taatcctccg
     1141 gcgaggggta ggggatgcat tcgtcaaagg ccatcaggat gtccgaaccg atctggtgtt
     1201 ggagccgggt ggcgtcctcg ggcgtcaact cgcggcggct gccgtccaag tgggattgga
     1261 actccacgcc ccgctccgtg atcttgcgca ggtccgccag gctgaagacc tggaatcccc
     1321 cgctgtcggt caggatgggc ttgtcccagt gcatgaaccg gtgcaggccg cccattttct
     1381 ccaacaggtc cgctcccggc cggagcatca gatggtaggt gttggccaac aggatctcgg
     1441 aaccgcaggc caata
//
LOCUS       DASTJJ010000750         1455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_470715, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000750 DASTJJ010000000
VERSION     DASTJJ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470715"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..581)
                     /locus_tag="VFR02_09045"
     CDS             complement(123..581)
                     /locus_tag="VFR02_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870626.1"
                     /translation="MKDPLRPAPARRPVEILLVEDNPGDVTLLRESIKRSRFPIHLCV
                     VEDGEKAIDFLRRREPYLAAPRPDFILLDLNLPRRDGFEVAQEVKRSSRLSGIPTLVL
                     TSSNNDIDRWKAVQHHLDAYLLKPQEWDQYAVLLKYLEENWMREIRPDIP"
     gene            complement(592..1038)
                     /locus_tag="VFR02_09050"
     CDS             complement(592..1038)
                     /locus_tag="VFR02_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870627.1"
                     /translation="MTNRSPADPNAVRILLVEDDLADAKLTLEILKKSSLPIEVERVK
                     DGVEALGYLRREGPYAGVKRPDLILLDLNLPRLGGLEALDRIKQDPELEPIPVLILTC
                     SQSDQDRLLAYESKANFFAVKPSDLEHFFEFVKYLENFWLKGLKSA"
BASE COUNT          252 a          452 c          468 g          283 t
ORIGIN      
        1 ctggccctgc ccctggcggc cttcgcgtcc gtgccgccct ccctggccgc cctggccatg
       61 gccctgtcct cgctctcggt ggtgctgaac tcgctcaggc tctacggcgt gaagaccgcc
      121 ccctagggga tgtcggggcg gatctcccgc atccagttct cctccaggta tttcaagagg
      181 acggcgtact ggtcccattc ctgcggcttc aataaatagg cgtccaggtg gtgctgtacg
      241 gccttccacc ggtcgatgtc gttgttggaa ctggtcagca ccagggtggg gatgccgctc
      301 aggcgggagg agcgcttgac ctcctgggcc acctcgaagc cgtcgcggcg ggggaggttc
      361 aggtccagca ggatgaaatc cgggcggggc gcggccaggt agggttccct ccggcgcagg
      421 aagtcgatgg ccttctcgcc gtcctccacc acgcagaggt ggatggggaa gcgggagcgc
      481 ttgatgcttt cccgcagcag ggtgacgtcg cccggattgt cctccaccag caggatctcc
      541 accggccgcc gcgcgggggc gggtctcaac ggatccttca tgacgcctcc ttcaggcgct
      601 tttcagcccc ttcagccaga aattttccag gtatttgacg aactcgaaga aatgctccag
      661 gtccgagggc ttcacggcga aaaaattcgc cttggactca taggccagca ggcggtcctg
      721 gtccgattgg gagcaggtga ggatcagcac cgggatcggc tccagttcgg gatcctgctt
      781 gatcctgtcc agcgcttcca ggccgcccag gcgcggcagg ttcaggtcca gcaggatcag
      841 gtcgggccgc ttcacgccgg cataggggcc ttcgcgccgg aggtagccga gggcctccac
      901 gccgtccttc acccgttcca cctcgatggg gaggctggat tttttcagga tctccagggt
      961 gagcttggcg tcggccaggt cgtcctcgac caggaggatg cgcaccgcat tggggtcggc
     1021 gggggaacgg ttggtcatga catgcctcct gggggggaac agccgggaaa gcggggccag
     1081 aggcaaaaga cccctcagtt ccctttggat ggtcgttaag agatccatgg ggattccttg
     1141 aagcaacttc acttgtccaa gcggtgtcga aaagaaggtc ggctgtcagg ccgacggtaa
     1201 tccaaggcga ttatcccccc ggcttgaaag ggcgcgggtc accaatcggt aaattcttct
     1261 gcaaaccttt tccctcgggt gacaaaaacc gggcgcctgg atccccgtcc ttaaaccgaa
     1321 cggtcgggtg ggtaaacccc ggacgcattt aacctgagcg cgctgaaaaa agcggggagt
     1381 ataatttttg gtagctggaa gtatcccgag cgccgccggg cgccgctcca acccctctcc
     1441 cggcctcacc ccctt
//
LOCUS       DASTJJ010000751         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1203585, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000751 DASTJJ010000000
VERSION     DASTJJ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203585"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFR02_09055"
     CDS             complement(<1..200)
                     /locus_tag="VFR02_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET9870628.1"
                     /translation="MRLDAVLFDLDGTLIDSKKDIAGAANAARVHFGMPALPLEVATT
                     YIGWGIDHLNKKALETEDPARLA"
     gene            410..1615
                     /gene="argJ"
                     /locus_tag="VFR02_09060"
     CDS             410..1615
                     /gene="argJ"
                     /locus_tag="VFR02_09060"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET9870629.1"
                     /translation="MKTPAPFPLPLGFTASGVHAGLKRPPKLDMGFLHSHRPAVWAGV
                     VTKNAVKSAPARRTEALLKKGKPLRAVVVNTKYANDLTGRQGYKDVEDTARWAAAGLR
                     HSPAEVLVHSTGVIGVPLPRAKVKRGVGLGLAALSGEGGGDFSRAILTTDLVPKTSAR
                     PLFPGGKPAWMVGFAKGSGMIHPNMATMLAYILTDAAVTRKVLAQALREAVSLSFNRI
                     TVDGDTSPSDSVVVLANGASGSKPITKASGPDYRELLEALKAVCLDLAQAVIRDGEGA
                     TKFVTVEIAGAKTDAQALWAAMAVAKSPLVKTAVFGADPNWGRILTAVGYSGASVDEF
                     KARVEIGGYRLYDGAPKKGWSRARLKAILQKKDIHIRVDLRLGRGACRVYTCDLTDGY
                     IDINGRYTT"
BASE COUNT          292 a          573 c          522 g          268 t
ORIGIN      
        1 gccagcctgg ccgggtcctc ggtctccagg gccttcttat tcaaatgatc gataccccag
       61 ccgatgtagg tggtggcgac ctccaaaggc agggcgggca tgccgaaatg cacccgggcg
      121 gcgttggccg ccccggcgat gtccttcttg gagtcgatca gggtcccgtc caggtcgaac
      181 aacacggcgt cgaggcgcat aaaggtcctt tgaacgaaaa cattttctcg cgcggggacg
      241 caaaggaagg cgaaactcgg atgggccccg ccgtccccgg gaaccgagaa actatatagc
      301 atccatccgc cgtcaggacc ggttttttcc cggcggttct catcatttga cacgattttt
      361 accaccccct acaatgcctc aacacccatc cccacccaag gaccccgaca tgaagacccc
      421 agcgcctttc cccctgcccc tgggctttac cgcctcgggc gtgcacgcgg ggctcaagcg
      481 cccgcccaag ctggacatgg gtttcctgca cagccaccgc ccggccgtct gggcgggcgt
      541 ggtcacgaag aacgccgtca agtccgcccc cgcgcggcgc acggaagccc tccttaaaaa
      601 agggaagcct ttacgggccg tggtggtgaa caccaaatac gccaacgact tgactggccg
      661 gcagggatat aaggacgtcg aggatacggc ccgctgggcc gccgccgggc tgcgccattc
      721 gcccgccgag gtgttggtgc attccaccgg cgtcatcggc gtgcccctgc cccgggccaa
      781 ggtcaagagg ggtgtgggcc tgggcctggc ggccctgagc ggcgaggggg gcggggattt
      841 ttcccgggcc atcctcacca ccgacctggt ccccaagacc agcgcccggc cgcttttccc
      901 gggcggcaag cccgcctgga tggtgggttt cgccaagggc tcgggcatga tccatcccaa
      961 catggccacc atgctggcct atatcctgac cgacgcggcc gtcacccgga aggtgctggc
     1021 ccaagccttg cgggaagccg tctccctgag cttcaaccgc atcacggtgg acggcgacac
     1081 ctcgcccagc gactcggtgg tggtgctggc caacggcgct tccggctcca agcccatcac
     1141 caaggcttcc ggccccgact accgggagct cctggaggcc ctgaaggccg tctgcctgga
     1201 cctggcccag gccgtcatcc gcgacgggga aggagccacc aagttcgtca ccgtggagat
     1261 cgcgggcgcc aagaccgacg cccaggccct ttgggccgcc atggcggtgg ccaagtcgcc
     1321 cctggtcaag accgccgtct tcggcgccga ccccaactgg ggccgcatcc tgaccgcggt
     1381 gggctattcg ggcgcgtcgg tggacgagtt caaggcccgg gtggagatcg gcggttaccg
     1441 gctctacgac ggcgcgccga aaaagggttg gtcgcgcgcg cggctcaagg ccatcctgca
     1501 aaagaaggac atccatatcc gggtggacct gcggctgggc cggggcgcct gccgggtcta
     1561 tacctgcgac ctgaccgacg gttatatcga catcaacgga cgctatacga cttgagattc
     1621 ataagcctct tcaccacgag cccctgcggg gccac
//
LOCUS       DASTJJ010000752         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1693115, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000752 DASTJJ010000000
VERSION     DASTJJ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693115"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFR02_09065"
     CDS             <1..499
                     /locus_tag="VFR02_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870630.1"
                     /translation="LAFAGDKLYIADTNNQAIRILDLGSGSVTTLALSGLDTPSPAPS
                     PEPAQALAAQTAAPGARELRVTIHLPEGRELNPQAPSRLELSSSDPAVARPERGSSPV
                     ESQTLTLPLSLHPGQATLTLHLTLFYCSHGRQALCFYKQDSLALPVTVSPQGAGELTV
                     DYRVP"
     gene            592..2286
                     /locus_tag="VFR02_09070"
     CDS             592..2286
                     /locus_tag="VFR02_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET9870631.1"
                     /translation="MSQPLSRLLEPIRSKTVMRLWLQWSGRRGLPWFVRGDLDGFFGL
                     FMDNLLQLMLIGGLCKAFCGFSADFITGQVLPAAALSILLGNLFYAWQARRLALKTRR
                     ADVTALPFGINTPSLIAFIFLIMAPIYQQTKDWRLAWQAGLFACLLNGLMEIGGAFVG
                     DWLRRNTPRAALLCALAGVAMTFISMGFIFQIFDHPLVGLVPMLLILVSYTSRVKWPL
                     SLPGGFFAVALGTLLAWIFHSLIGQPLSVSTDPIHLSLNAPKPVPGDLVALLADPTGW
                     KYLAVIFPMGLFNVVGSLQNLESAEAGGDRYPTKPSLLVNGLATLLAAFFGSAFPTTI
                     YIGHPGWKSMGARHGYSILNGLVISLLCLFGGVSLFLHVIPQECILGILLWIAIIITA
                     QAFQEIPREHALAVAFGLIPAFAAFTLELIIQPTLQAAGVPLALAIDKLEAAGLAIQG
                     VIYLNQGFLLTSMIFAAVLVHLIDRKFLKAAQWLFAAAGLSLVGLIHAYHLTVEGGLQ
                     NKFGVVAAPEFAFVYGLMGGLLLLLHYRSKSGGKSRRRKPKEAPPAPAPKRSVGYL"
     gene            2283..>2804
                     /locus_tag="VFR02_09075"
     CDS             2283..>2804
                     /locus_tag="VFR02_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HET9870632.1"
                     /translation="MRVGLAAVAALWLLASGARGQTPVPTPPAAAPAPVDEASFTWQQ
                     PSQADGILLYWSKVQGSDLVAFKGEGIVDAPLDKVATIIADTSRGTEWIDGLVKSQVV
                     DRASDTDFVEYDHQGVPFPFDQLISDRDFVSRVTLTADPNVHGLTIRYVSVQDPAAPP
                     PGKCVRGELRYCVF"
BASE COUNT          388 a         1046 c          857 g          513 t
ORIGIN      
        1 actggccttc gcgggggaca agctctacat cgccgacacc aacaaccagg ccatccggat
       61 cctcgacctg ggctcgggat cggtgacgac cctcgcgctc agcggcttgg ataccccttc
      121 gccggcgccc tcgcccgagc ccgcccaggc cctcgcggcc cagaccgccg cccccggcgc
      181 ccgggaactg cgcgtgacga tccacctgcc cgaagggcgg gagctgaacc cccaggcccc
      241 ttcccggctg gaactgtcct ccagcgaccc ggcggtggcc cggcccgagc gcggctcgtc
      301 gccggtggag tcccagaccc tgacccttcc cctctcgctc catccggggc aggcgaccct
      361 gacgctccac ctgacgctct tttattgttc ccacgggcgg caggcccttt gtttctacaa
      421 acaagactcc ctggccttgc ccgtgacggt ctccccccag ggcgccgggg aactcacggt
      481 ggactaccgc gtgccttgaa ggcctcgggc cgccccgttg gaagggaaag gtttcccccc
      541 aagggcccaa aatcgcttga ccggccccgg gggggccttt agtctttccc catgtcccag
      601 cccctttccc gcctgttgga gccgatccga tcgaagaccg tgatgaggct ttggctgcaa
      661 tggagcggcc gccggggcct gccctggttc gtgcggggcg acctggacgg ctttttcggc
      721 ctcttcatgg acaacctgct gcagctcatg ctcatcgggg ggctctgcaa ggccttctgc
      781 ggcttttcag ccgacttcat cacgggccaa gtgctgcccg cggcggccct gtccatcctc
      841 ttggggaacc tcttctacgc ctggcaggcc aggcgcctgg cgctcaagac ccgccgggcc
      901 gacgtgaccg cgctgccctt cggcatcaac acgcccagcc tgatcgcctt catcttcctc
      961 atcatggcgc ccatctacca acaaaccaag gactggcggt tggcctggca ggccggcctc
     1021 ttcgcctgcc tcttgaacgg cctgatggag atcgggggcg ccttcgtcgg cgactggctc
     1081 aggcgcaaca cgccccgggc ggccctcctg tgcgcgctgg ccggggtggc catgaccttc
     1141 atctcgatgg gcttcatctt ccagatcttc gaccatccgc tggtgggcct ggtgcccatg
     1201 ctgctcatcc tggtcagcta tacgtcccgg gtgaaatggc ccctgtccct gccgggcggc
     1261 ttcttcgccg tggccttggg cacgctcttg gcctggatct tccattcgct catcggccag
     1321 cccttgagcg tgtccaccga ccccatccac ctgtccctca acgcgcccaa gcccgtgccc
     1381 ggcgacctgg tggcgctgtt ggccgatccc acgggctgga aatacctggc ggtcatcttc
     1441 cccatgggcc tgttcaacgt ggtggggtcg ctgcagaacc tggaaagcgc cgaggccggc
     1501 ggcgaccgct accccaccaa accctcgctc ttggtcaacg gcctggccac cctgctggcc
     1561 gccttcttcg gcagcgcctt ccccaccacc atctacatcg gccatccggg atggaaatcc
     1621 atgggggccc ggcacggcta ttcgatcctg aacgggttgg tcatcagcct gctgtgcctc
     1681 ttcggcgggg tgtcgctgtt cctgcacgtc atcccccagg aatgcatcct gggcatcctt
     1741 ttatggatcg ccatcatcat cacggcccag gccttccagg agatcccccg ggagcacgcc
     1801 ctggcggtgg ccttcgggct catcccggcc ttcgcggcct tcaccctgga actgatcatc
     1861 cagcccaccc tgcaggccgc cggcgtcccc ctggccctgg ccatcgacaa actggaggcc
     1921 gcgggcctgg ccatccaggg cgtcatctac ctgaaccagg gcttcctctt gacctccatg
     1981 atcttcgcgg cggtgttggt ccacttgatc gaccgcaagt tcctgaaggc ggcccagtgg
     2041 ctcttcgcgg ccgccggatt gtcgctggtg gggctcatcc acgcctacca cctgaccgtg
     2101 gaagggggcc tgcagaacaa gttcggggtg gtggccgcgc ccgagttcgc cttcgtctac
     2161 ggcctgatgg ggggcctgtt gctgttgctg cactaccggt ccaagtcggg cggcaagtcc
     2221 cgccgccgca aacccaagga ggcgcccccg gcgcccgcgc ccaagcgcag cgtggggtac
     2281 ctgtgagggt gggcctcgcc gccgtcgccg ccctttggct cttggcttcc ggggcccggg
     2341 gacagacccc cgtccccacg cccccggccg ccgcgccggc cccggtggat gaggcttcct
     2401 tcacctggca gcagccctcc caggccgacg gcatcctgct ttactggtcc aaagtgcagg
     2461 gcagcgacct ggtggccttc aagggggagg gcatcgtgga cgcgccgctc gacaaggtgg
     2521 ccaccatcat cgccgacacg tcccggggca ccgagtggat cgacgggctg gtgaagtcgc
     2581 aagtggtgga ccgggcctcg gacaccgact tcgtggaata cgaccaccag ggcgtgccct
     2641 tccccttcga ccagctcatc tcggaccggg acttcgtgtc gcgggtgacc ctcacggccg
     2701 atcccaacgt gcacggcctg accatccgct acgtgtccgt gcaggacccg gccgcgccgc
     2761 ctcccgggaa atgcgtgcgg ggggagttgc gctactgcgt cttc
//
LOCUS       DASTJJ010000753         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_470921, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000753 DASTJJ010000000
VERSION     DASTJJ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470921"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1689)
                     /locus_tag="VFR02_09080"
     CDS             complement(<1..>1689)
                     /locus_tag="VFR02_09080"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET9870633.1"
                     /translation="PQVFLRRLPPPAAMAPASATPQASAAIPLPPFKKILVRDGQVFF
                     QTDPDKEAQQVAGAIRLQAQSEDARRWGIWIEAKPPDPKSSGSLRITGSLNLEDPRLV
                     GKADLKDWDLASFGPLLRSFTGWNLLGGRANLEVPFAYRPGGNPWFDIRGHVEDASVR
                     APGENGVLFTGIEGRTSIRPTGLALNQPLRFQVGQTSFQASGVIPFDGLPVSLAVSSD
                     SLYLSTLVGDVLHLKNLKSDGVGRADLTVRGSLSDPVLQGSAELGTSHLGDWRLDSLA
                     LSAQYAHGVFQLTQVTGRLYDGILTASGQVLVGPGQEPVSLQATLEKVQAKPVAALLG
                     LKGMEGTTDAHMELSGTLQEPQFSSNVSMDLVRTLRKTLYHYSIQNDVQLKDKKLVLS
                     VTLNGEARLLAQFNEGPDGWEFGKLSMANGKQLIHFQGSGFFPKDVDAPMRLEIHGQD
                     IALQDLVFFKDQFPGVRGRVGMDLVLSGTRNAPKATLEFRSRDADLGKGLTEGPLAVS
                     LDWAGDQLTIRKLDYQNPSGRSFSIQGTLGLQSDSPMDLRVQADRMPLTLLTEI"
BASE COUNT          296 a          559 c          559 g          275 t
ORIGIN      
        1 tgatctcggt caacaaggtc aagggcatcc ggtcggcctg gaccctgagg tccatgggcg
       61 aatcggactg caaccccagc gtcccctgga tggaaaagct gcggcccgag gggttttgat
      121 agtccaactt ccggatggtc agttgatccc ccgcccaatc cagcgaaacg gcgagcgggc
      181 cttcggtcag tcccttgccc aggtccgcgt cccgggatcg gaattccagg gtggccttgg
      241 gcgcgttccg ggtcccggaa agcaccaggt ccatccccac ccgtccccgt accccgggga
      301 actggtcctt gaaaaagacc aggtcctgca gggcgatgtc ctgcccatgg atctccaggc
      361 gcatgggcgc gtccacatcc ttcgggaaga aaccggaccc ttggaaatgg atcaattgct
      421 tgccgttggc catcgacaac ttgccgaact cccagccatc gggcccttcg ttgaactggg
      481 ccaacagccg ggcttccccg ttcaaggtca ccgaaagcac cagcttcttg tccttcaact
      541 ggacgtcgtt ctggatggaa taatggtaaa gggtcttgcg cagggtgcgg accaggtcca
      601 tggagacgtt ggaggagaat tggggctcct gcagggtccc gctcagttcc atgtgggcgt
      661 ccgtggtccc ctccatgccc ttcaatccca ggagggcggc caccggcttg gcctggacct
      721 tttccaaggt ggcctgcagg gaaacgggtt cctggcccgg ccccaccagg acttggccgc
      781 tggccgtcag gatgccgtca taaagccggc ccgtgacctg ggtcagttgg aagacgccgt
      841 gggcgtactg ggcggaaagc gccagcgaat ccagccgcca gtcccccaag tgggacgtcc
      901 ccaactcggc ggacccttgc agcaccgggt ccgacaacga gccccggacc gtcaggtcgg
      961 cccggcctac cccgtcggac ttcaggttct tgaggtgcaa cacgtccccg accagggtgg
     1021 aaaggtacag gctgtcggac gagaccgcca gggacaccgg caagccgtcg aaagggatca
     1081 ccccggaggc ctggaaggag gtctgcccca cctggaaccg caagggttgg ttcaaggcca
     1141 ggcccgtggg acggatggac gtgcggccct cgatgcccgt gaagagcacg ccgttctccc
     1201 ccggcgcccg gacgctggcg tcctccacgt gcccccggat gtcgaaccag gggttccccc
     1261 cggggcggta ggcgaagggc acttccaggt tggcgcgccc acccagcagg ttccaacccg
     1321 tgaaggaccg caacagcggt ccgaagctgg ccaggtccca atccttcagg tcggccttcc
     1381 ccaccaaccg gggatcctcc aggttcaggc tgcccgtgat cctcaaggag ccggacgact
     1441 tggggtcggg cggcttcgcc tcgatccaaa tgccccaacg gcgggcgtcc tcggactggg
     1501 cctgcagtcg gatggccccg gccacctgct gggcctcctt gtcgggatcg gtctggaaga
     1561 agacctgtcc atcccgcacc aggatcttct tgaagggcgg cagggggatc gcggccgacg
     1621 cctggggcgt ggcggaggcc ggggccatgg cggcgggcgg cggcagacgg cgcaggaaga
     1681 cctggggac
//
LOCUS       DASTJJ010000754         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_227815, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000754 DASTJJ010000000
VERSION     DASTJJ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227815"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFR02_09085"
     CDS             <1..857
                     /locus_tag="VFR02_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET9870634.1"
                     /translation="MTQGAPLNLPDGPLRQKTSFYYALLLLPRRRRLAMETLYRFCWA
                     ADEVSDGPGTPAQKRKDLAAFRAGLTACLAGRPRGPLFTALRRVVEEFHLSAEPLRRT
                     VAGVERDLRPLKFRAFAELHRYARQVAGGPGLSSMEIFGFRDPAHRLYAENLGVFLQI
                     VNIVRDYREDLALGRQYLPQEDFRRFGLHPDRLDPRDLAWRPFVEFQLDRALGFLDLA
                     RHALTRRQRAGLATAEAIAAVYGRLERKLRARPQAILAGKVSLSPAEKLAAVGGALWR
                     CARWRVWP"
     gene            854..2182
                     /gene="hpnE"
                     /locus_tag="VFR02_09090"
     CDS             854..2182
                     /gene="hpnE"
                     /locus_tag="VFR02_09090"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET9870635.1"
                     /translation="MSPRALVVGGGYAGVAAATALAEAGWGVDLLESRGFLGGRVYSI
                     VNDRFPLPVDNGPHLLMGCYREAFRLFDRLGLKDGFHRVDPLALRWISPGGGAVSLQC
                     PPLPAPFHLIAGILASDAFPGTEKWKLMASLAPFAVRPFPLPRRARTVSDWMRLTRQG
                     TVTRERFWVPLCRAVMNVPPEIAPLRGLGEVLRRVFFGSRKDSALVVPKRPLSEIAFP
                     QVPGYLRARGGEVHLREGARSLRLASGPFQVGTVTGKTFTADALVLALPPRSLEALWN
                     SSGLPSPAQEARLGRSPILSVHLIVEKKVVEGQMVGLPGAAFDWVFNRDANWGYAGPG
                     QYLSLVSSADPDLAARPEKEVVVRAWEEMQARFPALAGTQPLHAKVTREMAATFFWDE
                     AAGAARLPAATAFPNVFLAGDWTATGLPATIEGACLSGHRAAAGLLGRRS"
     gene            complement(2179..>3070)
                     /locus_tag="VFR02_09095"
     CDS             complement(2179..>3070)
                     /locus_tag="VFR02_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9870636.1"
                     /translation="DGGPDRKKKMAWAIGVTSAILVLEAVGGVWSGSLALLSDAGHML
                     TDLAALLISLTAMILAERPASPTHTFGLARLEVLAALGNALTFFLMVAAVAWQAGDRL
                     LHPALPDWRAMGAIAVVGFLANAANAGLLHGAHQEDLNLRGAWLHVMGDLASSLGVLV
                     GVVLIARTGWSWVDPVLSLLIALLIAGSALRLFRKALRILLESSPRGLSAEDISQSLR
                     EGLPEIQEVHHVHLWEVGAGQVYLTAHLVVTDRTIGDGMALLGRAQALLKEKLQIDHS
                     TFQLEVHVSPRVPLSEKSRP"
BASE COUNT          429 a         1117 c         1039 g          485 t
ORIGIN      
        1 acatgaccca aggagcgcct ttgaacctcc cggacggacc cctgcgccag aagaccagct
       61 tctattacgc cctgctgctg ttgccccggc ggcggcggct ggccatggaa accctctatc
      121 gcttctgttg ggccgccgac gaggtctccg acgggccggg cacgccggcc caaaaacgga
      181 aggacctggc ggccttccgc gccgggctca ccgcctgcct ggcgggccgg ccccgggggc
      241 cgctcttcac ggcgctgcgg cgggtggtgg aggaattcca tctttccgcc gagccgctgc
      301 ggcgcacggt cgctggcgtg gagcgggacc ttcgccccct gaaattccgc gccttcgccg
      361 aactgcaccg ttacgcccgc caggtggccg ggggcccggg gctctccagc atggagatct
      421 tcggtttccg ggaccccgcc caccggctct acgcggagaa cctgggcgtc ttcctgcaga
      481 tcgtcaacat cgtccgcgac taccgggagg acctggcctt gggccgccag tacctgcccc
      541 aggaggattt ccgccgcttc gggctccatc cggaccggtt ggatccccgc gatcttgcct
      601 ggcggccttt cgtggagttc caattggacc gcgccctcgg gttcctcgac ctggcgcgcc
      661 atgcgctgac ccgccgccaa agggccgggc tcgccaccgc ggaggccatc gcggccgtct
      721 acggccgact ggagcgcaaa ctgagggccc gcccccaggc catcctggcg gggaaggtct
      781 ccctgtcccc cgccgagaaa ctggcggccg tgggcggcgc cttatggcgt tgcgcccgct
      841 ggagggtctg gccgtgagcc cccgggccct ggtggtgggg ggcggttacg cgggcgtggc
      901 ggcggccacc gcgctggccg aggcggggtg gggcgtggac cttttggaat cccgcggctt
      961 cctgggcggg cgggtctatt ccatcgtgaa cgaccgcttt cccctgcccg tggacaacgg
     1021 cccccacctg ctcatgggct gctaccggga ggctttccgg ctcttcgacc gcctgggcct
     1081 gaaggacggg ttccaccggg tggacccctt ggcgctgcgt tggatctccc ccggcggcgg
     1141 cgcggtgtcg ctccaatgcc ccccgctccc cgcgcccttc catctgatcg ccgggatcct
     1201 cgcctccgac gccttccccg gcacggaaaa atggaagctc atggcatccc tcgcgccctt
     1261 cgcggtgagg cctttccccc tgccccgccg ggcccgcacg gtgtcggatt ggatgcgcct
     1321 cacccgccaa ggcaccgtca cccgggagcg cttctgggtc cccctctgcc gcgcggtgat
     1381 gaacgtgccg cccgaaatcg cgccgttaag gggcctgggg gaggtgctgc ggcgggtctt
     1441 tttcgggtcg cggaaggaca gcgccctggt ggttcccaag cgtcccttga gcgagatcgc
     1501 cttcccccag gtccccggct acctgcgggc ccggggcggc gaggtgcatc ttcgggaagg
     1561 ggcccggtcc ctgcgcctgg cctccggccc cttccaagtg gggaccgtca cgggcaagac
     1621 cttcacggcg gacgccctgg tcctggccct gccgccccgc tccttggaag ccttgtggaa
     1681 ttcctccggc cttccctcgc ccgcccaaga agcgcgcctg ggccgttcgc ccatcctgtc
     1741 cgtgcacctg atcgtggaaa aaaaggtcgt ggaaggccag atggtgggtt tgcccggcgc
     1801 ggccttcgac tgggtcttca accgcgacgc caactggggt tatgcgggcc cgggccagta
     1861 cctgagcctg gtctccagcg cggacccgga cctggcggcc cggcccgaga aggaggtcgt
     1921 ggtgcgggcc tgggaagaga tgcaggctcg gttccccgcc ctcgccggca cccaaccgtt
     1981 gcacgccaag gtcacgcggg aaatggcggc gacctttttt tgggatgagg ccgccggggc
     2041 cgcccgcctg cccgccgcca cggcctttcc gaacgtgttc ctggccgggg actggaccgc
     2101 caccgggctc cccgccacca tcgagggtgc ctgcctgtcg ggccaccggg ccgccgcggg
     2161 gcttttgggg cgccggtcct aaggccggga cttttcgctc aggggcaccc ggggggacac
     2221 gtggacctcg agttgaaagg tgctgtggtc gatctggagc ttttccttca ggagggcctg
     2281 ggcccgcccc agcaacgcca tcccgtcgcc gatggtccgg tccgtcacca ccaggtgggc
     2341 cgtcaggtag acctggcccg cccccacttc ccagagatgg acatggtgca cttcctggat
     2401 ctcgggcaat ccctcccgga ggctttggga aatgtcctcg gccgataatc cccgggggga
     2461 ggattccaac aggatgcgca gggccttgcg gaaaagccgc aaggcgcttc ccgcgatcaa
     2521 caaggcgatc aagaggctca ggaccgggtc cacccaagac cagcccgtgc gggcgatgag
     2581 gacgaccccc accaacaccc ccaaggagga ggccaagtcc cccatcacgt gaagccaggc
     2641 cccccgcaag ttcaggtcct cctggtgggc cccatgcagc aacccggcgt tggcggcgtt
     2701 ggccaggaaa cccacgacgg cgatggcgcc catggcccgc cagtcgggca acgccgggtg
     2761 cagcagccga tcgcccgcct gccaagccac ggcggccacc atgaggaaaa aggtcagcgc
     2821 gttgcccaag gccgccagca cctccaggcg cgccaggccg aaggtatggg tggggctggc
     2881 gggccgttcg gccaggatca tggccgtgag ggagatcagg agggccgcca ggtcggtcaa
     2941 catgtggccc gcgtcggaca aaagcgccag gctgcccgac caaacgccgc ccacggcctc
     3001 caagaccagg atcgcggacg tcaccccgat ggcccacgcc atttttttct tccggtccgg
     3061 gccgccgtcc
//
LOCUS       DASTJJ010000755         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1203916, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000755 DASTJJ010000000
VERSION     DASTJJ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203916"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFR02_09100"
     CDS             <1..319
                     /locus_tag="VFR02_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET9870637.1"
                     /translation="SLRGQLVHRSPDMPQRTRDFYQWMDDHAPGFGFCRTYKGGRGFI
                     QDEPWHWSYQPYSRLYTAQMGSLGDIRAILQDKVEGVDYVLRHFPEIYQGLEDSVDPA
                     CCR"
     gene            complement(330..1205)
                     /locus_tag="VFR02_09105"
     CDS             complement(330..1205)
                     /locus_tag="VFR02_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9870638.1"
                     /translation="MPRPRVLIVYKESAYTRNLPSRKLAAHFRKGDYWDVLRDSHDRH
                     HSSLDVVKRALSREGCPVDLALRNRIHRLRGLDRRYGLVVSVGGDGTLLEVSHHLRRI
                     PVLGVNSDPRHSVARFSGCDAASFPRLLEAHLGGRAPRLEVARLAFSVNGRPNPWLVL
                     NEVLVAAANPAGTSRYLLEVGGRAEEQLSSGVWIGPAAGSTAALLSAGGRRLPLGSNR
                     FQFVVREAYHRKFGARRLLKGVLGPRVRVRMVSLMRQGRIFLDGANLEFPFKLGDRLE
                     IGLSPFPLKVVGLTA"
     gene            complement(1223..1804)
                     /locus_tag="VFR02_09110"
     CDS             complement(1223..1804)
                     /locus_tag="VFR02_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET9870639.1"
                     /translation="MTFAQLLSLFLPIFVAMDAVGVLPSFVQMTSGMRDRDRRDLLRY
                     SVLTAGLVGLLFIPLGPWVLSALGLKPGDFQVAGGLLILVVALRDIVWDQKMSLGAGA
                     KGSGVVPLGIPLLVGPAVLTTLVLLLGRFSDLEVAAALAVNLLLAWLVFGYAARLIRW
                     MGEPGARVASKLAALLLSAYAVMMIRVGLSSLK"
     gene            complement(1846..>2169)
                     /locus_tag="VFR02_09115"
     CDS             complement(1846..>2169)
                     /locus_tag="VFR02_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870640.1"
                     /translation="PSLPCVAYLGNGDLLDFDRVPPDQDFFPTSLQAKSGPVSTLEAG
                     DVYCLKLFTMAGHAWIRVTDPGVEASTAPGYGPSFAFRVNQSLPYYAYDRTAADLAGT
                     CGASW"
BASE COUNT          396 a          748 c          752 g          273 t
ORIGIN      
        1 gagcctgcgg gggcaattgg tgcaccgctc ccccgacatg ccccagcgca cgcgggactt
       61 ctaccaatgg atggacgacc acgcccccgg tttcggcttt tgccgcacct ataagggggg
      121 ccggggcttc atccaggacg agccctggca ctggagctac cagccctatt cgcgcctcta
      181 cacggcgcag atgggatccc tgggggacat ccgggcgatc ctgcaggaca aggtcgaggg
      241 ggtggactac gtgttgaggc acttcccgga gatctaccag ggcctggagg attcggtcga
      301 ccccgcctgc tgccgctgac gggtccccgt caggccgtca ggccgaccac cttcaaggga
      361 aaaggcgaca ggccgatctc caaccggtcc cccagcttga aggggaactc caaattggcg
      421 ccgtccagga agatccggcc ctggcgcatg agcgagacca tccggacccg gacccggggc
      481 cccagcacgc ccttcaagag ccgccgggcg ccgaacttgc ggtggtaggc ctcgcgcacc
      541 acgaactgga accggttgga tcccaggggc aaccggcgcc cccccgcgga caagagcgcc
      601 gccgtggaac ccgcggccgg gccgatccag acgccgctgg aaagctgttc ctccgcccgc
      661 ccgcccactt ccagcaggta ccgggacgtc cccgccgggt tggcggccgc caccagcacc
      721 tcgttgagca ccagccaggg attggggcgc ccgttcaccg aaaaggccaa ccgcgcgacc
      781 tccaggcggg gcgcccggcc gcccaggtgg gcctccaaaa gccgcgggaa ggaggcggcg
      841 tcgcagccgc tgaagcgggc cacgctgtgc cgcgggtccg aattcacccc cagcacgggg
      901 atgcggcgca ggtggtggga cacctccaac agggtgccgt ccccgcccac cgacaccacc
      961 aggccgtagc ggcggtccag gccccgcagg cggtggatgc ggttgcgcag cgccaggtcc
     1021 accggacagc cttcccgcga cagggcgcgt ttcaccacat ccaggctgga atggtgccgg
     1081 tcatggctgt cgcgcagcac gtcccaatag tcgcccttgc ggaaatgggc ggccagcttg
     1141 cgggacggca ggttgcgggt gtaggcgctt tccttgtaga cgatgaggac gcggggtcgg
     1201 ggcatggcct ttttcacggg ttttatttga gggacgacaa gccgacccgg atcatcatga
     1261 ccgcgtaggc ggacaagagc agcgccgcca acttggaggc gacccgggcc ccgggctcgc
     1321 ccatccaacg gatgaggcgg gcagcatagc cgaaaaccag ccaggccagc aacaggttca
     1381 ccgccagggc ggccgccacc tccaggtccg agaagcgtcc caggagcagc accagggtgg
     1441 tcagcaccgc cgggcccacc agcagcggga tgcccagggg caccacgccc gatcccttcg
     1501 cgcccgcgcc cagggacatc ttttggtccc acacgatgtc ccgcagcgcc accaccagga
     1561 tgagcagccc ccccgccacc tggaaatcgc cgggcttgag gcccagggcc gaaagcaccc
     1621 aggggcccaa ggggatgaag agcagcccca ccagccccgc ggtgaggacc gaataacgca
     1681 gcaggtcccg gcggtcccgg tcgcgcatgc ccgaggtcat ctgcacgaag gacggcagca
     1741 cgcccacggc gtccatggcc acgaagatgg gaaggaaaag gctgagcagt tgggcgaagg
     1801 tcatggggcc attctaaccc aacccgtccc ccggggggga cgggatcacc aggacgcgcc
     1861 gcaggtgccg gccaggtccg cggccgtccg gtcgtaggcg taatagggga ggctttggtt
     1921 gacccggaag gcgaagctgg ggccgtagcc gggcgcggtg gaagcttcca cccccgggtc
     1981 ggtcacccgg atccaggcgt ggcccgccat ggtgaagagc ttgaggcaat aaacgtcgcc
     2041 ggcctccagc gtggagaccg ggccggattt ggcctgcagg gaggtcggga aaaaatcctg
     2101 gtcggggggc acgcggtcga aatccagcag gtccccgttg cccaggtagg cgacgcaggg
     2161 caggctggg
//
LOCUS       DASTJJ010000756         4606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_348893, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000756 DASTJJ010000000
VERSION     DASTJJ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348893"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..372)
                     /locus_tag="VFR02_09120"
     CDS             complement(118..372)
                     /locus_tag="VFR02_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9870641.1"
                     /translation="MKRDWAARGFSCGLWTDPPGAVWEDYVHPTDELVMLLEGQVELE
                     MKGRRIRLEKEKEVLIPAGTVHSVRNRGTGTSYWLYGYHR"
     gene            complement(395..1954)
                     /locus_tag="VFR02_09125"
     CDS             complement(395..1954)
                     /locus_tag="VFR02_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9870642.1"
                     /translation="MKKAIGCLFAVILLSAAPLFAGDESLRQSVVKIFTVTNKPNYYQ
                     PWVIGYQFEASGSGCILPGKRILTNAHVVSDEVFIQVMKAGDTKKYTAEVEYVDHDRE
                     VAVLRVKDGSFFKGTRPVEFGALPSQRDKVAVYGFPIGGDELSITEGVVSRIEVQRYV
                     HSQRDFLTIQTDAAINPGNSGGPAFEGGKLIGIAFQSYGGDTAQGIGYIVPMPVIERS
                     LKDRAAGNLTGAPSLGILWQKMESDSLRDFYKMKPDQSGVLVTQVVYGSPADGVVKAR
                     DVLLSEAGVPIANNGTIPFAGGNRLAFSYLTSRYANGDQVPLEVLRDGKVVKLTLKLK
                     PFDPLVAGPAYDVRPSYYIFGGIVFMPLSWDYLNLWKSGTAPSQLMDLYENGLPSPSR
                     KQVVFINEVLPDDLNVGYHDLKQVVVDKIDGMPITQLSDVPLAFQHPIHGYHVIELDE
                     TEGDGADAGGQVVFAADGAEKATREIMKSFGISKDRSDDLLGDGVTPLLPEGGAPVTN
                     TPGALGGKLHL"
     gene            complement(1995..3977)
                     /gene="acs"
                     /locus_tag="VFR02_09130"
                     /pseudo
     CDS             complement(1995..3977)
                     /gene="acs"
                     /locus_tag="VFR02_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4264..>4606
                     /locus_tag="VFR02_09135"
     CDS             4264..>4606
                     /locus_tag="VFR02_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9870643.1"
                     /translation="MKILVAPDKFKNVNSSQKMAELIRNAIQKELPEVIIRLCPLADG
                     GEGTLNAIVYSLKGKTVAAPSFGPLMKPLQAHYGLTQLPEKNSALIEMALVSGLSLLS
                     PEERNPMNTTTY"
BASE COUNT          776 a         1545 c         1462 g          823 t
ORIGIN      
        1 atgcccggca tgccgtcgca caggaggatg acccggccct tccgggcgtc cttggccggc
       61 gtccagaact cggccacgat ctcttttttg aatcgggtgc ggtacaggga aggctcctta
      121 ccgatggtat ccatacaacc aataggaagt gcccgtcccc cggttgcgca cgctgtggac
      181 cgtcccggcc gggatgagga cttccttctc tttttccaag cggatcctgc ggcctttcat
      241 ttccagttcg acttggcctt ccaaaagcat caccagctca tcggtcgggt ggacgtaatc
      301 ctcccagacc gcgcccggcg ggtcggtcca aaggccgcag ctgaagccac gggcggccca
      361 gtcccttttc acggactccg ggatgcccat ggccctacag gtgcagcttg ccgcccaggg
      421 cgccgggggt gttcgtcacg ggcgccccgc cctcgggcag gagcggcgtg acgccgtccc
      481 ccagcaggtc atccgagcgg tccttggaaa tgccgaagga cttcatgatc tcccgggtgg
      541 ccttctcggc cccgtcggcc gcgaagacca cctggccgcc cgcgtcggcc ccgtcgccct
      601 cggtctcgtc caattcgatg acgtggtagc cgtggatggg gtgctggaag gccaacggca
      661 cgtcggacaa ctgggtgatg ggcatgccgt cgatcttgtc caccaccacc tgcttgaggt
      721 cgtggtagcc cacgttcagg tcgtcgggca gcacttcgtt gatgaacacc acctgcttgc
      781 gggacggcga gggcaggccg ttctcgtaaa ggtccatcaa ttgggaaggc gcggtccccg
      841 atttccacag gttcaggtag tcccagctca agggcatgaa cacgatcccg ccgaagatgt
      901 aataggaagg ccgcacgtca taggcgggcc cggccaccag cgggtcgaag ggcttgagct
      961 taagggtgag cttcaccacc ttcccgtccc gcagcacttc caggggcacc tggtcgccgt
     1021 tggcgtagcg gctggtcagg taggaaaagg ccaaccggtt gccccccgcg aaggggatgg
     1081 tgccgttgtt ggcgatgggg acccccgcct cggacaagag cacgtcccga gccttcacca
     1141 ccccgtccgc gggggaccca tagaccacct gggtcaccaa cacgccggac tggtcgggct
     1201 tcatcttgta gaagtcccgc agggagtcgc tttccatctt ttgccagagg atgcccaggc
     1261 tgggcgcccc ggtcaggttg cccgccgcac ggtccttgag cgaccgctcg atgaccggca
     1321 tgggcacgat atagccgatc ccctgggcgg tgtcgccccc gtaggactgg aaggcgatgc
     1381 cgatgagctt gcccccctcg aaggccggcc cccccgaatt gcccgggttg atggccgcgt
     1441 cggtctggat ggtcaggaaa tcgcgctggg aatgcacgta gcgctgcacc tcgatgcggg
     1501 acaccacgcc ctccgtgatg gacaactcgt cccccccgat ggggaaccca tagaccgcca
     1561 ccttgtcccg ttggctgggc agggcgccga attccaccgg acgggtgccc ttgaagaagg
     1621 acccgtcctt cacccgcagc accgccacct cccggtcgtg gtccacatac tcgacctcgg
     1681 cggtgtattt cttggtgtcc cccgccttca tgacctggat gaagacctcg tcggacacca
     1741 cgtgggcgtt ggtcaggatg cgcttgcccg gcaggatgca gcccgagccg ctggcctcga
     1801 attggtagcc gatcacccag ggctggtaat aattgggctt gttggtgacc gtgaagatct
     1861 tcaccaccga ctggcgcagg gactcgtccc ccgcgaagag gggcgcggcc gacagcagga
     1921 tcacggcgaa caggcaccca atggcctttt tcatatggtt ttttctcgct ttcatggata
     1981 acggcccgag cggctcactg cctgccctgc accaggttct gcaccaccga aggatcggcc
     2041 agggtggtca cgtcgcccag gttccccaac tcgttctccg cgatcttgcg caggatgcgg
     2101 cgcatgatct tgccgctgcg ggtcttgggc agccccgggg cgaactggat cttgtccggc
     2161 tgggcgatgg ggccgatctc acggcgcacg tgcgcgacca gttcctgctt gagcccctcg
     2221 gaaagcgccg cgccctcctt cagggtcacg taggcgtaga gggcctgtcc cttgatgtcg
     2281 tggggatagc ccaccacggc ggcttcggac accagcgggt gggacaccag ggcgctttcc
     2341 acctcggcgg tgccgatgcg gtgccccgag atgttcacca cgtcgtccac ccgcccgagc
     2401 agccaatagt ccccgtcggc gtccacccgg cagccgtcgc ccgtgaaata gacgtcgggg
     2461 aacgccgaaa agtagacgtc catgaaacgc tggtggtcgc cccaggtggt gcgcatcatg
     2521 cctggccagg gtttcttgac gcagagcttg ccgccctcat tgggcccgca ttcggtgccg
     2581 tcgtcgcgca gcaccacggg ctccaccccg aagaagggcc gggaggcgct gcccggtttg
     2641 agcgcgtggg cgcccgggaa gggcgcgatc aaaatgccgc ccgtctcggt ctgccaccag
     2701 gggtccacga tgggtcagcg gccgcgcccc acctgccggt ggtaccaaag ccaggcctcg
     2761 gggttgatgg gctcgcccac cgaccccagc agccgcagcg aatccaactt gtacttttgg
     2821 ggccaggact ccccttcccg catgagggcc cggatggccg taggggccgt gtagaaaagg
     2881 ctgacccggt gcttgtccac cacctgccag aagcggcccg ggtccggata ggtgggcacg
     2941 ccctcgaaca tgagcgtggt ggcgccgttg gcgagcggcc cgtacacgat gtagctgtgc
     3001 ccagtcaccc agccgatgtc cgccgtgcac caatagacgt cgcccggccg gtggtcgaag
     3061 atgtactggt gggtcagggc cgcgtgcagc aggtagcccg ccgtggtgtg cagcacgccc
     3121 ttgggctttc ccgtggagcc cgaggtgtag aggatgaaga aggggtcctc ggcgtccatc
     3181 acctccgggt cgttcgaaaa gggggcgcgg gccgtctcct catgccacca ggaatcccgg
     3241 cccggcgtca tggcggtccg gccgtccccc cgttggaaca cgatgacgtt cttcaccgtg
     3301 gggcagtcct tcagcgcctc gtccacgatg gccttcaggg ggatggtctt ccccgcccgg
     3361 aagcccacgt cggcggtcag caccagcagg ttctgggaat cctggatgcg gctcttgagc
     3421 gactcggcgc tgaaacccgc gaagaccacc gaatggatgg cccccacgcg cgcgcaggcc
     3481 agcagggcca ccgccgcctc gggcaccatg ggcatgtaga cgcagacccg gtcgcctttt
     3541 ttcacgccct tggccttcag gatgttggcg aattggcagg tcatctggtg cagttcgtcg
     3601 taggagaggg cccggacctc ctcgtccttt tccccctgcc agaggatggc cgtttgaccg
     3661 cccttgcccg acaggagatg ccggtccagg caattatagg aggcgttcag ttgcccgtcg
     3721 gcgaaccagg tgtgttccac cacccgggcc ttcgtatccc aggtatattt cagggtctgg
     3781 gtgggcttct tgaaccagct gagttccttc agggcctgct ccaaccaaaa accgtggggg
     3841 tccttcagcg accggtcgta aagcgcctgg tattcggccg gggacttcac gtgggcccgg
     3901 gcggcgaaat ccggcggtgg ggggaaaaaa gccgcttcct tttggagcga caggatgaaa
     3961 ttttcgggtt ctggcatggc gcctccttat ccggtccggg ggccggcgcc tttgattgta
     4021 cccgccccac cgcccccggc caggcgaaaa accttgaaaa aaaccaggaa aagcgtcccg
     4081 ggccgggcct tcccccggcc acaaacctat gccggaaggc ttcctggatg taaaataaac
     4141 gggccgccaa tcccgtttcc cggcgcccgg gaggcggcgt tgagggtccg ccccggaccg
     4201 tcggccgccc gcttcgaccg ttgccgtcct tggatgcctc taaaaacgtt cgctggagac
     4261 cgcatgaaaa tcctggtcgc gcccgacaag ttcaagaacg tgaacagctc gcagaagatg
     4321 gccgagctga tccgcaacgc gatccagaag gaactgcccg aggtcatcat ccgcctctgt
     4381 cccctggccg acggcggcga aggcaccttg aacgccatcg tgtattcgct caagggcaag
     4441 accgtggccg cgccctcctt cggcccgctc atgaagcccc tccaggccca ctacggcctc
     4501 acccagttgc ccgagaagaa ttcggcgctg atcgagatgg ccctggtgtc cggcctgtcg
     4561 ctgctgtccc ccgaggaacg caaccccatg aacaccacca cctacg
//
LOCUS       DASTJJ010000757         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_592046, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000757 DASTJJ010000000
VERSION     DASTJJ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592046"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1070)
                     /locus_tag="VFR02_09140"
     CDS             complement(<1..>1070)
                     /locus_tag="VFR02_09140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9870644.1"
                     /translation="NALQDWASKLTCLGWVAAAAFLISALLAGLLEIEWIGTPFYAAS
                     GAVFLFGVLPFFLSDAWSHTFAATAFALTVLTLGAWWAWVSRWGRVVLAAGVIWASFA
                     SLPHGGLQYTDYVSLMILGRVWAAFFSTATILAVYAMVLRFYGKPGLALLASAFFAFS
                     VSSIQVAHYCITESFITLMLVVVALLSRRIFEKGDWASYLTAGAAFGISMAAKTSSLY
                     YVLILVMAHLLFLSRKKAAEWVREDRKHADNRDVFTLLAGGLLVVVAVVFAGVVYKLK
                     GVLQDLFYNGNQQVLPPAWILMMLLAVGAGLVLALWGLKEFKAVRGQIPYWLRLTAAG
                     ALSFLMFCLFSPWSLLDFTGFQ"
BASE COUNT          269 a          333 c          337 g          131 t
ORIGIN      
        1 tttggaaccc ggtgaaatcc aaaagggacc agggggaaaa gaggcagaac atcaggaagg
       61 agagggcgcc cgcggccgtc aagcgcagcc aataggggat ctgcccccgc acggccttga
      121 attccttcaa accccagagc gccaagacca gtcccgcgcc gacggccaaa agcatcatca
      181 ggatccaagc ggggggaagg acctgttggt tcccattgta aaagaggtcc tggaggacgc
      241 ccttgagctt gtagaccacc ccggcaaaga caacggccac gaccaccagc aacccgccgg
      301 cgagcaaggt gaaaacatcc cggttgtccg cgtgctttcg gtcctcccgg acccattcgg
      361 cggccttttt ccgcgacaaa aagagcaggt gcgccatcac caggatcagc acgtagtaca
      421 acgaactggt cttggcggcc atggaaatgc cgaaggccgc ccccgcggtc aagtagctgg
      481 cccagtcccc cttttcgaag atgcgccggg ataaaagggc gaccaccacc agcatcaggg
      541 tgatgaagga ctcggtgatg caataatgcg ccacttggat gctggacacc gagaaggcga
      601 agaaggccga cgccaagagg gccaacccag gcttgccgta gaaccgcagg accatggcgt
      661 agaccgccag gatggtggcc gtggagaaaa aggccgccca gacgcggccc aggatcatca
      721 ggctcacgta atccgtatat tgaagcccac catgcggcag cgaggcgaac gaggcccaga
      781 tcacgcccgc cgccaggacc acccttcccc agcgggaaac ccaggcccac caagcgccca
      841 gggtcaggac cgtcagggca aaagccgtgg cggcgaaggt gtggctccag gcgtccgaca
      901 ggaaaaaggg cagcacgccg aagaggaaca ccgccccgct ggcggcgtaa aagggcgtgc
      961 cgatccattc gatctccaag aggccggcca aaagggccga gatcaggaaa gcggcggcgg
     1021 cgacccaacc cagacaggtc agtttggagg cccagtcctg gagggcgttg
//
LOCUS       DASTJJ010000758         1212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_592054, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000758 DASTJJ010000000
VERSION     DASTJJ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592054"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..842
                     /locus_tag="VFR02_09145"
     CDS             234..842
                     /locus_tag="VFR02_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET9870645.1"
                     /translation="MPTLVLVRHGQSQWNLENRFTGWVDVPLTALGEEEAHRAGQHLK
                     GLKFDVAFTSELQRAQKTLAVILEETSQTGIPILKDQALNERHYGDLQGLNKAETAKQ
                     YGDDQVKIWRRSYDVPPPKGESLKDTAARTIPYFERTIVPYLKEGKNVIVAAHGNSLR
                     SIVMHLEHLTKEQVLELNLETAVPRVYELGPDLKILSSKDLK"
     gene            890..>1212
                     /locus_tag="VFR02_09150"
     CDS             890..>1212
                     /locus_tag="VFR02_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870646.1"
                     /translation="MGLALAGGCSPRLVARPEPAAVPPPRAVAPEARPVPTALPTPPP
                     LTKAQLLGFQAPPDLGTDPPTGKKAQAAVIDAYEQLKALAAQDGWRLILVSGYRSYWH
                     QVSVW"
BASE COUNT          221 a          441 c          366 g          184 t
ORIGIN      
        1 gtggtgaaat gggttttgtt agaagttctt aatttccccc cgcacccatc ccctctccct
       61 cgaaggggag aggggatggg aaaccgacgt ttacgctctc cctctccctt gacgggagag
      121 ggcggggtga gggtggatta aactgagacg ctaccccttt gccctttcca aaccgggtcc
      181 cagcggataa aatcaccctt cgatttcccg gaaaacgcgc aaaggggtgt gaaatgccga
      241 ctctcgtcct ggtccgccac ggccaatccc agtggaacct ggagaaccgc ttcacgggct
      301 gggtggacgt gcccctcacg gccctgggcg aggaggaagc ccaccgggcc ggccaacacc
      361 tgaagggcct gaagttcgac gtggccttca cgtccgagct gcagcgggcc cagaagaccc
      421 tggccgtcat cctggaggag acctcccaga ccggcatccc catcctgaag gaccaggccc
      481 tgaacgagcg ccactacggc gacctgcagg ggctcaacaa ggccgagacc gccaagcagt
      541 acggcgacga ccaggtcaag atctggcgcc gcagctacga cgtgccgccg cccaagggcg
      601 agtcgctgaa ggacaccgcc gcgcgcacca tcccctattt cgagcggacg atcgtcccct
      661 atttgaagga aggcaagaac gtcatcgtgg ccgcccacgg caattccctg cgctccatcg
      721 tcatgcacct ggaacacctc accaaggaac aggtgctgga gctgaacctg gagaccgccg
      781 tgccccgggt ctacgaactg ggcccggacc tgaagatcct ctcgtccaag gacctgaaat
      841 agcggcctcg cgaccgggac gcccgtcccc ccggcgcctt ctgctgctcg tgggcctggc
      901 cctggccggg ggctgttcgc cccgcctggt ggcgcgcccc gagcccgccg ccgtcccccc
      961 gccgcgcgcc gtagcccccg aggcccggcc cgtccccacc gccctgccca cgccgccgcc
     1021 gctcaccaag gcccaattgc tgggcttcca agcgccgccg gacctgggca ccgacccacc
     1081 gaccggcaag aaggcccagg ccgccgtcat cgacgcctat gagcaattga aggccctggc
     1141 cgcccaggac ggctggcggc tgatcctggt ctcgggttac cggtcctatt ggcaccaggt
     1201 ctcggtctgg aa
//
LOCUS       DASTJJ010000759         5238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_592085, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000759 DASTJJ010000000
VERSION     DASTJJ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592085"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFR02_09155"
     CDS             complement(<1..199)
                     /locus_tag="VFR02_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET9870647.1"
                     /translation="MITIKSKDELELMRKSGRVVGEALVELGKRVKPGVTTEELDRFA
                     YSFFTKNGCVPAFKGYHGYPST"
     gene            complement(196..840)
                     /locus_tag="VFR02_09160"
     CDS             complement(196..840)
                     /locus_tag="VFR02_09160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9870648.1"
                     /translation="MRTILLGEPGAGKGTYSLELVKRYGIPQISTGDMLRAAVRAGTP
                     LGLKAQEYMKQGALVPDSVVIGLIKERIAQPDAAKGFILDGFPRTVPQAQSLDETLKE
                     LGISLSSVFKIEVSRATLIKRLTGRRVCKACGTTYNIDTMRPQKEGVCDKCGGELIQR
                     PDDMPATIENRLSVYQKDTAPLIEYYEKKGLLRRASCEGEYEDVIARIFAALEK"
     gene            complement(837..2165)
                     /gene="secY"
                     /locus_tag="VFR02_09165"
     CDS             complement(837..2165)
                     /gene="secY"
                     /locus_tag="VFR02_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9870649.1"
                     /translation="MLSAFTNIFRIPELKSRVLFTFGMLLVYRVGCHIPTPGVNPAAL
                     QNFFSNQNGGILGFLNLFSGGALRQFSVFALGVMPYISMSIILQLLTAVIPALEKLSK
                     EGDAGRRKITQYTRYGTVALCLVQGTGTTYLLKSLGDNIVYHWGLGFQLLTVLTLTAG
                     TIFIMWIGEQITEYGIGNGISIIIFAGIVASLPEQIFNTVRLMRLGEVSILGVLFFAA
                     FTLALIAFIVVLQQGARKIPVQYATRVVGKQVFKGQSTQLPLKVDYSGVIAVIFSSSI
                     LVFPTTLARYLQSVAPEDGWLYGAMDFVTRNFSPGAFIYYVLYSALIVFFCYFYTAIA
                     FNPTDLSENMKKYGGFIPGIRPGQPTAEFIDRTLTRITIWGALAVCCVAVFPDALTRS
                     MKIPIYFGGTSLLIIVGVGLDTMKQIESHLLMRHYDGFMKKGRLKGRTAF"
     gene            complement(2166..2603)
                     /gene="rplO"
                     /locus_tag="VFR02_09170"
     CDS             complement(2166..2603)
                     /gene="rplO"
                     /locus_tag="VFR02_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9870650.1"
                     /translation="MKMNQLKPARGAVRRRKIVGKGPGSGHGKTSTRGHKGQNSRTGG
                     GVRLGFEGGQTPLYRRIPIRGFKSLDLSIPSVVNLDRLAIFADGAVVTPVELKAKGLV
                     KNVDQVKILGKGELKLKLTVKAHAFSASALEKIKAAGGVTEVL"
     gene            complement(2623..2799)
                     /gene="rpmD"
                     /locus_tag="VFR02_09175"
     CDS             complement(2623..2799)
                     /gene="rpmD"
                     /locus_tag="VFR02_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9870651.1"
                     /translation="MSEIKIQLVKSLNGRLPRHRRIAEALGLNKIRQTVVKPDTPVIR
                     GMVEKIGYLVEVKK"
     gene            complement(2796..3308)
                     /gene="rpsE"
                     /locus_tag="VFR02_09180"
     CDS             complement(2796..3308)
                     /gene="rpsE"
                     /locus_tag="VFR02_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9870652.1"
                     /translation="MGMIDASKFDLKEKVVNINRVAKVVKGGRRFSFAVLVVVGDGHG
                     HVGAGTGKASEVPDAIRKAVEDAKKNLIEVPLKNSTLYHEMMGVFKASRVMLRPAAPG
                     TGIIAGGGVRAVCELAGIHDVLTKSIRSDNVFNVVQATLTGLKAIRSPETISKLRGKP
                     VESLVGGAKS"
     gene            complement(3343..3717)
                     /gene="rplR"
                     /locus_tag="VFR02_09185"
     CDS             complement(3343..3717)
                     /gene="rplR"
                     /locus_tag="VFR02_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9870653.1"
                     /translation="MSVLISQIRRKKRAKRHARIRKTVKGTAVRPRLVVNRGSKNIAA
                     QLIDDTKGVTLAAASSFDANLRSKLKYGGNLEAAKAVGEQIAKLAKEKGLAQAVFDRG
                     GYAFHGRIKALADAARQGGLQF"
     gene            complement(3727..4284)
                     /gene="rplF"
                     /locus_tag="VFR02_09190"
     CDS             complement(3727..4284)
                     /gene="rplF"
                     /locus_tag="VFR02_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9870654.1"
                     /translation="MARIAKKPIEIPAGVKVKIEGQKVTLEGPKGSETHEVLPGLKVE
                     LQGQQLLVSITGNERALGAVHGLVRALLKNIVNGVAKGFERKLEIQGVGYRAAMEKGK
                     LSMSLGFSHPVVMELPKGVSVDIEKQTMLTVKGANKYLVGETAAKIRRVKPPEVYKGT
                     GIRYLGEQVRKKVGKTGATGKAGGK"
     gene            complement(4312..4713)
                     /gene="rpsH"
                     /locus_tag="VFR02_09195"
     CDS             complement(4312..4713)
                     /gene="rpsH"
                     /locus_tag="VFR02_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9870655.1"
                     /translation="MSMTDPIADMLTRIRNANHAYKEKVDVPFSKLKLEIARILSEEG
                     FIKNYKVIEEKGKPSAIRISMKYGEKRERVITHIGRVSTSGRRVYRGKAELPRVIGGL
                     GVAVLSTSKGILTDQQARQQGVGGEVLCLIH"
     gene            complement(4727..4912)
                     /locus_tag="VFR02_09200"
     CDS             complement(4727..4912)
                     /locus_tag="VFR02_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9870656.1"
                     /translation="MAKKSLVNKQQKKPKFKVRAYSRCKLCGRPRAFLRRFGMCRICF
                     RNLADEGKIPGVVKASW"
     gene            complement(4921..>5238)
                     /gene="rplE"
                     /locus_tag="VFR02_09205"
     CDS             complement(4921..>5238)
                     /gene="rplE"
                     /locus_tag="VFR02_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695058.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9870657.1"
                     /translation="SNFKLRADQPLGCKVTLRGDRMYQFLERLFMVALPRIRDFRGVP
                     TRSFDGRGNYTLGLKEQIIFPEINFENVQSIHGMDITFATTARTDEEARELLKLLGMP
                     FRN"
BASE COUNT          894 a         1674 c         1627 g         1043 t
ORIGIN      
        1 tggtggaggg gtacccatgg taccccttga aggccggcac acagccgttc ttcgtgaaaa
       61 acgaataagc gaagcggtcc agctcctccg tggtcacccc cggcttcacc cgcttgccca
      121 gctcgaccag ggcttccccg accacccggc cgctcttgcg catcagctcc aactcatcct
      181 tggacttgat cgtgatcact tctcaagggc cgcgaaaatg cgggcgatca cgtcctcgta
      241 ctcgccctcg caggaggccc gccgcagcag gccttttttc tcgtaatatt cgatcaaagg
      301 cgcggtgtcc ttttggtaca ccgacagccg gttctcgatg gtggccggca tgtcgtcggg
      361 gcgctggatg agctcgccgc cgcacttgtc gcacacgcct tccttttggg ggcgcatggt
      421 gtcgatgttg taggtcgtcc cgcaggcctt gcagacccgg cggcccgtga gccgcttgat
      481 gagggtggcc cggctcacct cgatcttgaa gaccgaggag aggctgatgc ccaattcctt
      541 caaggtctcg tccaggctct gggcctgggg cacggtccgg gggaatccgt ccaggatgaa
      601 acccttggcc gcgtcgggct gggcgatgcg ttccttgatc aggccgatca ccaccgaatc
      661 cggcaccaag gcgccctgct tcatgtattc ctgcgccttc aatcccaagg gggtccccgc
      721 ccgcacggcc gcccgcagca tgtcgccggt ggagatctgg gggatgccgt aacgcttcac
      781 cagctccaac gaataggtcc ccttgcccgc gccgggctcg cctagaagaa tggtcctcaa
      841 aacgcggtcc tccctttcaa gcggcccttc ttcatgaaac cgtcataatg ccgcatcagg
      901 aggtgcgatt cgatctgttt catggtgtcc aggcccacgc ccacgatgat cagcagggac
      961 gtcccgccga aataaatggg gatcttcatg gagcgggtca gggcgtcggg gaagaccgcc
     1021 acgcagcaga ccgccaaggc gccccaaatg gtgatgcggg tgagcgtgcg gtcgatgaac
     1081 tcggccgtgg gttggccggg ccggatgccg gggatgaagc cgccatactt cttcatgttc
     1141 tcggacaggt cggtcgggtt gaaggcgatg gccgtgtaga agtagcagaa gaacacgatc
     1201 agggccgagt acagcacgta gtagatgaag gcgccgggcg agaagttgcg ggtcacgaaa
     1261 tccatggcgc cgtagagcca gccgtcctcg ggggccaccg actgcaggta gcgggccagg
     1321 gtggtcggga agaccaggat ggaggaggaa aagatgacgg cgatgacgcc cgagtaatcc
     1381 accttgagcg gcagctgggt ggactgtccc ttgaagacct gcttgcccac cacccgggtg
     1441 gcgtattgca cggggatctt gcgggccccc tgctgcagca ccacgatgaa ggcgatgagc
     1501 gccagggtga aggccgcgaa gaagagcacg cccaggatgc tgacctcgcc caagcgcatc
     1561 aagcgcacgg tgttgaagat ctgctcgggc aggctggcca cgatgccggc gaagatgatg
     1621 atggagatgc cgttgccgat cccgtactcg gtgatctgct cgccgatcca catgatgaag
     1681 atggtgccgg cggtgagcgt caggacggtc aagagctgga accccaggcc ccagtggtac
     1741 acgatgttgt cgcccagcga cttgagcagg taggtggtgc cggtgccctg caccaggcag
     1801 agcgccacgg tgccgtagcg ggtgtattgg gtgatcttgc ggcggcccgc gtcgccttcc
     1861 ttggagagct tttccaaggc cgggatgacg gcggtcaaga gctgcaaaat gatggacatg
     1921 ctgatgtagg gcatcacgcc cagggcgaag accgagaact gccgcaaggc gccgcccgag
     1981 aagaggttca ggaagcccag gatgccgccg ttctggttgg aaaagaagtt ctgcagggcc
     2041 gccggattga cgcccggcgt ggggatatga cagcccaccc ggtagaccag cagcataccg
     2101 aaggtgaaaa ggacccggct tttgagttcc gggatccgga agatgttggt gaaggccgaa
     2161 agcacttaca agacctccgt cacgccgccg gcggccttga tcttctccag ggccgaggcg
     2221 ctgaaggcat gggccttcac ggtcaacttg agcttcaact cgcccttgcc caggatcttg
     2281 acctggtcga cgttcttgac caagcccttg gccttgagct ccacgggcgt gaccacggcc
     2341 ccgtccgcga agatggccag gcggtccagg ttgaccaccg aggggatgct caggtccagg
     2401 ctcttgaagc cccggatggg gatgcgccgg tacagggggg tctggccgcc ctcaaagccc
     2461 aggcggacgc cgccgcccgt gcgggaattc tggcccttgt ggccgcgggt cgaggtcttt
     2521 ccatggcccg agcccgggcc cttgccgacg atcttccggc ggcgcaccgc gccccgggcg
     2581 ggtttcaatt ggttcatctt catgacgttg ttcctcggtc ccttattttt tcacttcgac
     2641 cagatagccg atcttctcga ccatgccccg gatgaccggg gtatcgggtt tgaccaccgt
     2701 ttggcggatc ttgttgaggc ccagggcctc cgcgatgcgg cggtgccgcg gcagcctgcc
     2761 gttcaggctc ttcaccagtt ggatcttgat ctcgctcatg acttcgcgcc ccccaccagg
     2821 ctttccaccg gcttgccccg cagcttggag atggtctccg gggaacggat ggccttcaag
     2881 cccgtcaagg tggcctgcac cacgttgaac acgttgtccg aacggatgga cttggtcagc
     2941 acgtcgtgga tgcccgcgag ctcgcacacc gcgcgcacgc ccccgccggc gatgatgccc
     3001 gtcccgggcg cggcgggccg cagcatcacc cgcgaagcct tgaagacgcc catcatctca
     3061 tggtaaaggg tgctgttctt caacggcacc tcgatcaggt tcttcttggc gtcctcgacg
     3121 gccttccgga tggcgtccgg gacctcactg gccttgcccg tgcccgcgcc cacgtgcccg
     3181 tggccgtcgc cgaccaccac caagacggcg aagctgaacc ggcgtccgcc cttgaccacc
     3241 ttggccacgc ggttgatgtt gaccaccttt tccttcaagt cgaacttgct cgcgtcgatc
     3301 attcccatac ggattccttt ttgcctttca aactggttgt ggttagaact gcagtccgcc
     3361 ctggcgcgcc gcgtccgcca aggccttgat gcggccgtgg aaggcgtaac cgccgcggtc
     3421 gaacaccgcc tgggccaggc ccttttcctt ggccagcttg gcgatctgct cgcccacggc
     3481 cttggccgct tccaggttgc cgccgtattt gagcttcgag cgcaggttgg cgtcgaagct
     3541 ggaggcggcc gccagggtca cgcccttcgt gtcgtcgatc aactgggcgg cgatgttctt
     3601 ggacccgcgg ttcaccacca gccgggggcg gacagccgtg cccttgacgg tcttgcggat
     3661 acgcgcgtgg cgcttggccc tctttttgcg ccggatctga gaaataagaa cggacatggt
     3721 ggctccttac ttgccgccgg ctttgccggt ggcgccggtc ttgccgactt tcttgcggac
     3781 ttgctcgccc agataacgga tccccgtgcc tttgtagacc tcggggggct tcacccggcg
     3841 gatcttggcg gcggtctcgc cgaccaggta cttattggcg cccttcacgg tcaacatggt
     3901 ctgtttctcg atatccaccg agacgccctt gggcagttcc atgaccaccg gatggctgaa
     3961 ccccagcgac atgctgagct tccccttctc catggccgcg cggtaaccca cgccctggat
     4021 ctccagtttg cgttcaaacc ccttggccac cccgttgacg atgttcttca acaaggcccg
     4081 gaccaggccg tgcacggcgc ccagggcccg ttcattcccg gtgatggaca ccagcagctg
     4141 ctgcccctgc agctccacct tgaggcccgg cagcacctcg tgggtctcgg agcccttggg
     4201 cccttccagg gtcaccttct gcccttcgat cttcaccttg accccggcgg ggatctcaat
     4261 cggtttcttg gcaatgcgcg ccatgacgtc tcccttactt gatgaacaag atcaatgaat
     4321 caaacaaagc acttcgccgc ccacgccctg ttggcgggcc tgctggtcgg tcaagatgcc
     4381 cttggaggtg gacagcacgg ccacgcccag gcccccgatg accctgggaa gctcggcctt
     4441 gccgcggtag acccggcggc ccgaggtgct cacccggccg atatgggtga tcacgcgctc
     4501 gcgcttctcg ccgtacttca tggagatgcg gatggccgag ggcttgccct tctcctcgat
     4561 cactttgtag ttcttgatga agccttcctc cgagaggatg cgggcgatct caagcttcag
     4621 tttggaaaag gggacgtcca ccttttcctt gtacgcgtgg ttcgcgttgc ggatccgcgt
     4681 cagcatgtcc gcgatggggt cggtcatcga catggttctt tctcccttac cagctggcct
     4741 tgacgacgcc ggggatcttc ccctcgtccg ccaggttcct gaaacagata cggcacatcc
     4801 cgaaacgcct caagaaggcc cgagggcgtc cgcagagctt gcaacggctg taggcccgga
     4861 ccttgaactt gggcttcttt tgctgcttgt tgaccagact tttcttcgcc atgaattctc
     4921 ctagttccta aaaggcattc ccagcagctt caacagctcc ctggcttcct cgtcggtcct
     4981 cgccgtggtg gcgaaggtga tgtccatgcc gtggatggac tggacattct cgaaattgat
     5041 ctcggggaag atgatctgct ccttcaaccc cagggtgtag ttgccccggc cgtcaaagga
     5101 acgggtcggc accccgcgga agtcgcggat gcggggcagc gccaccatga aaaggcgctc
     5161 caggaactgg tacatgcggt ccccccggag ggtcaccttg cagcccaagg gctggtcggc
     5221 ccgcagcttg aagttcga
//
LOCUS       DASTJJ010000760         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_961786, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000760 DASTJJ010000000
VERSION     DASTJJ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961786"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1030
                     /locus_tag="VFR02_09210"
     CDS             <1..>1030
                     /locus_tag="VFR02_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870658.1"
                     /translation="GVDGVLVASMDGRSLESIPATPPLVDPSYAASDEFKSVVEHLKG
                     QPGGIYQFYTRKTSSPSFVFALGLAPGTAGEVVFDLGALLKGANLHGGEAYILDGKSG
                     QYLYATNASRYDQVFNPSQDPALAKVMTDLAAGTGGAAQADGSRILYTPFLNSYGLVE
                     QIPESAFTAAPAPAEAGSPLERLQTPVSVAALVALAWVFLMFYTGLNLLMAPLKKARA
                     VVLEALQNGKSINPEVVQRFGKDEVGQIVQAASDLLKKQETEKLELERDKEESIRQAR
                     ALIENKNKEAQQVQQQADGVKRQLEDSQQQLGDKLKELEALKGMAEGLRGQTEQSKAD
                     NAKLRTQLA"
BASE COUNT          193 a          358 c          336 g          143 t
ORIGIN      
        1 ggcgtggacg gcgtgctggt ggcctccatg gacggccgca gcctggaatc catcccggcc
       61 acgccgcccc tggtggaccc ttcctacgcc gcctcggacg agttcaagag cgtggtggag
      121 cacttgaagg gccagccggg cggcatctac cagttctata cccgcaagac ctcctcgcct
      181 tccttcgtct tcgcgctggg gctggccccc ggcaccgccg gggaagtggt gttcgacctg
      241 ggggcgctcc tcaagggcgc gaacctgcac ggcggcgagg cctatatcct ggacggcaag
      301 tcgggccagt acctctacgc caccaacgcg tcccgctacg accaggtctt caacccctcg
      361 caggaccccg cgctggccaa ggtgatgacc gacctggcgg cgggcacggg cggcgcggcc
      421 caagccgacg gcagccggat cctctatacg cctttcctga attcctacgg cctggtcgag
      481 cagatccccg agtcggcctt caccgccgcc cccgcgccgg ccgaggccgg aagccccctg
      541 gagcggctgc agacgcccgt gagcgtggcg gccctggtgg ccttggcctg ggtgttcctc
      601 atgttctata ccggcctcaa cctcttgatg gcccccctca agaaggcccg cgccgtggtt
      661 ttggaggccc tgcagaacgg caagtccatc aaccccgagg tggtccaacg cttcggcaag
      721 gatgaggtgg gacagatcgt gcaggcggcc tccgacctgc tcaagaagca ggaaacggag
      781 aagctcgagc tggaacggga caaggaagaa tccatccggc aggcccgggc gctcatcgag
      841 aacaagaaca aggaagccca gcaggtccag cagcaggccg acggggtcaa gcgccagttg
      901 gaggacagcc agcaacagct cggcgacaag ctgaaggaac tggaggccct caagggcatg
      961 gccgagggcc tgcgcggcca gaccgagcag tccaaggccg acaacgccaa gctccgcacc
     1021 caactggcca
//
LOCUS       DASTJJ010000761         1372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1572277, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000761 DASTJJ010000000
VERSION     DASTJJ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572277"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1351
                     /locus_tag="VFR02_09215"
     CDS             143..1351
                     /locus_tag="VFR02_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9870659.1"
                     /translation="MPKAKMSVNNLQGQLLALNRIGIALTRENNLNQLLALILTESRN
                     FTGAEAGSLYVREGEQLRFAVSQNEVMEERKKAELKKLKKPLEVVPATFTGFYISLDK
                     SSLAGYVGVTGKTLNLPDAYKIPSSKEYRFNRSFDEKNNYRTKSMLIVPMKDAHGDVV
                     GVIQLINARKGKTIVPFDKHFENLVLSLASQAAIAIRNALLTQELKEAYLDTIFRLSV
                     AAEYKDDDTAAHIHRMSRYSSILAEGLGLSPAEVESIQYASPMHDIGKLGVPDSILMK
                     PGKLTPQEFKEMQNHTIFGAKILENAKADLLRVSEQIALTHHEKWDGSGYPRGLKGEG
                     IPLSGRIVALADVFDALTSKRCYKPAFPLEQSLDIIKEGSGRHFDPKVVQAFLNNIEK
                     FLAVKAQFSN"
BASE COUNT          307 a          464 c          373 g          228 t
ORIGIN      
        1 aaatcagcgc tttcctctcc cgctaagggc ctttgaaccg atttgaagca ggtttttcgg
       61 gcccccgccg ccctccggaa cccttgtaaa agccggtctt ttcaaggtat gttgaacgct
      121 cgaatcaccc caaggaagcg ccatgcccaa agccaaaatg agcgtcaaca acctccaggg
      181 acagctgttg gccctgaacc gcatcggcat cgcccttacc cgcgagaaca acctcaacca
      241 actgctggcc ctcatcctca ctgaaagccg caacttcacc ggcgccgagg cgggcagcct
      301 ttacgtgcgc gaaggggaac agttgcgttt cgccgtcagc cagaacgagg tgatggaaga
      361 acgcaagaag gccgagctga agaagctgaa gaagccgctg gaagtggtgc cggccacctt
      421 cacggggttc tacatctcgc tggacaagag ttccttggcg ggctacgtgg gcgtgacggg
      481 caagaccctc aacctgcccg acgcctacaa gatcccctcc tcgaaggaat accgcttcaa
      541 ccgctccttc gacgagaaga acaattaccg caccaagagc atgctcatcg tgcccatgaa
      601 ggacgcccac ggcgacgtgg tgggggtcat ccaactcatc aacgcccgca agggcaagac
      661 catcgtcccc ttcgacaagc atttcgagaa cctggtcctg tccctggcca gccaggccgc
      721 catcgccatc cgcaacgccc tcctgaccca ggaattgaag gaagcctacc tggacaccat
      781 cttccgcctg tcggtggccg ccgaatacaa ggacgacgac accgccgccc acatccaccg
      841 catgagccgc tattcgtcca tcctggccga ggggctgggg ctttcacccg ccgaggtgga
      901 gagcatccaa tacgcctcgc ccatgcacga catcggcaag ctgggcgtgc cggattcgat
      961 cctcatgaag ccgggcaaac tcacgcccca ggaattcaag gagatgcaga accacaccat
     1021 cttcggcgcc aagatcctgg agaacgccaa ggccgacctc ttgcgggtct ccgagcagat
     1081 cgccctgacc caccatgaga aatgggacgg cagcggttac ccccggggcc tgaagggcga
     1141 gggcatcccc ctgtcgggcc gcatcgtggc cctggcggac gtctttgacg cgctcacgtc
     1201 caagcgctgc tacaaacccg ctttccccct ggaacagtcg ctggacatca tcaaagaggg
     1261 gagcggccgc catttcgatc ccaaggtggt ccaggccttc ctgaacaaca tcgagaaatt
     1321 cctggcggtc aaggcgcagt tcagcaactg accgcctcct tgccggggct tg
//
LOCUS       DASTJJ010000762         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1449321, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000762 DASTJJ010000000
VERSION     DASTJJ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449321"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1405)
                     /gene="secD"
                     /locus_tag="VFR02_09220"
     CDS             complement(104..1405)
                     /gene="secD"
                     /locus_tag="VFR02_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET9870660.1"
                     /translation="MTLQRFVALAVLVLIAASVYAIYPPSQKLKLGLDIKGGLHMNLE
                     VDESQMPGKTPAEISDAADRALEILRNRVDGLGVSEPVLEREGPAGIVIQLPGLTDIK
                     RAKDILLSTAQLEFRLVSTEDIANYKGKDGLVDEKRLPKGVTCLPGKDGEQYLVEKTL
                     LTGDSLVDAHVQPDQYGRPIVEFKLNADGGKKFGILTGDNIHRQLAIILDHRVYSAPT
                     IQSRIAGGSGIITGSFTDEEARDLALVLRAGALPAPLKITGQFQVGPTLGADSVKKGT
                     WAALLGLVLVLLFMGIYYGLSGWIANVGLLINMLFLLASLAGLQATLTLPGIAGIILT
                     LGLSVDSNVLIFERIREELRLGKTIRAAIEAGYHRALVAIIDTHVTTLISAAVLFQFG
                     TGPIKGFAVTLSLGVGLSLFTAVVVTKMIFDARKNYQSLSI"
     gene            complement(1510..1953)
                     /locus_tag="VFR02_09225"
     CDS             complement(1510..1953)
                     /locus_tag="VFR02_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9870661.1"
                     /translation="MSRALAVLGLVLGLAAVPAHPAQAPGVVTITPDNFKAEVLGSSL
                     PVLVDVGATWCVPCRLLNPLVDKMAVEYAGRLKVVRVDADANGDLVDNLGIQAFPTLL
                     FFKKGKVVKTAVGLETEKELRSQIDRVLGPASAAKKAPSGPGQMH"
     gene            complement(1965..2294)
                     /gene="trxA"
                     /locus_tag="VFR02_09230"
     CDS             complement(1965..2294)
                     /gene="trxA"
                     /locus_tag="VFR02_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9870662.1"
                     /translation="MGATLKTTDASFKQDVLESKVPVLVDFWAEWCGPCKMVGPLLDE
                     LAQEYDGKLRIAKVNVDENQQVSVDFHIRSIPTLLFFKNGQLVKQLIGAHPKNKLVGE
                     IQEVIGA"
BASE COUNT          430 a          725 c          733 g          425 t
ORIGIN      
        1 ccgccaacat gcccaacccc gacagggcga ggaacaggcg gcgcactccc atgaagttga
       61 tcttgggttc cttaaaccat tgaatcatgt cggggccttt cggttaaatg ctgaggcttt
      121 ggtagttctt gcgggcgtcg aagatcatct tggtgaccac cacggcggtg aagaggctca
      181 agcccacccc caggctcaag gtgacggcga aacccttgat ggggcccgtg ccgaactgga
      241 aaaggaccgc cgccgagatg agcgtggtca cgtgggtgtc gatgatggcc accagggccc
      301 ggtgatagcc ggcctcgatg gccgcccgga tggtcttgcc caggcgcagt tcctcgcgga
      361 tgcgctcgaa gatcagcacg ttggaatcca ccgagagccc cagggtcagg atgatgcccg
      421 cgatgccggg cagggtgagg gtggcctgca ggccggcgag ggaggccaac aggaaaagca
      481 tgttgatcaa caaccccacg ttggcgatcc agccggacag gccgtaatag atccccatga
      541 acagcagcac caggaccagg cccaagaggg ccgcccaggt ccccttcttg accgagtccg
      601 cgcccagggt ggggcccacc tggaactggc cggtgatctt cagcggggcg ggcagggcgc
      661 ctgcccgcag taccagggcc aggtcccgcg cctcctcatc ggtgaagctt cccgtgatga
      721 tgccggagcc tcccgcgatg cggctctgga tggtgggcgc cgaatagacc cggtggtcca
      781 ggatgatggc caactggcgg tggatgttgt cgcccgtcag gatgccgaat ttcttcccgc
      841 cgtccgcgtt gagcttgaat tccacgatgg gccgcccgta ctggtcgggt tgcacatggg
      901 cgtccaccaa cgaatcgccg gtcaggaggg tcttttcgac caggtactgt tccccatcct
      961 tgccgggcag gcaggtgacg cccttaggca ggcgcttctc gtccaccagg ccgtccttac
     1021 ccttgtaatt ggcgatgtcc tcggtggaga ccagccggaa ctccaactgg gcggtggaca
     1081 gcaaaatatc cttggcgcgt ttgatgtccg tcaggccggg cagttggatg acgatgcccg
     1141 cgggcccttc ccgttccagg accggctcgc tcacgcccaa cccgtccacg cggttgcgca
     1201 ggatctccag ggcccggtcc gccgcgtccg agatctcggc cggcgtcttg ccgggcatct
     1261 gggattcgtc cacttccagg ttcatgtgca ggccgccctt gatgtccagg cccagcttca
     1321 atttctggga aggcgggtag atggcataga cggaggccgc gatcaggacc aggacggcga
     1381 gcgccacgaa acgttggagg gtcacaaaaa acctttcgtc gtcaaatagg atcgatcgaa
     1441 ccgaggcccg gaagggcctg aaaaaggggg taaagcggag ggcagtatag ggaatccctt
     1501 aaaaagctgt caatgcattt ggccgggccc cgaaggggcc tttttagcgg cggaagcggg
     1561 ccccaggacc cggtcgatct gggagcgtag ttccttttcg gtctcaagcc cgaccgcggt
     1621 cttgacgact ttccccttct tgaaaaaaag gagggtggga aaggcttgga tgcccaggtt
     1681 gtccaccagg tccccgttgg cgtcggcgtc cacgcggacc accttgagcc tgcccgcgta
     1741 ttccaccgcc atcttgtcca ccaaagggtt cagcagccgg cagggcacgc accaagtggc
     1801 gccgacgtcc accagcacgg gcagggagga tcccaggacc tcggccttga agttgtccgg
     1861 ggtgatcgtg accacgccgg gcgcctgggc gggatgcgcg gggacggccg ccaagcccag
     1921 gaccagcccc aagaccgcca gcgcgcggga caaggccgtc cgcctcacgc tccgatgact
     1981 tcctggatct cgccgaccaa cttgttcttg ggatgggccc cgatcagctg cttgaccaac
     2041 tgtccattct tgaagaacaa cagcgtgggg atgctgcgga tatggaagtc gacggacacc
     2101 tgctggttct cgtccacgtt caccttggcg atcctcaact tgccgtcgta ttcctgggcc
     2161 agttcgtcca acagggggcc caccatcttg cagggcccgc accattcagc ccaaaagtcg
     2221 accagcaccg ggactttgga ttccaacacg tcctgtttga agctcgcgtc cgtcgttttc
     2281 agggtcgctc ccattctccg cctccattgt ttt
//
LOCUS       DASTJJ010000763         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_471897, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000763 DASTJJ010000000
VERSION     DASTJJ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471897"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFR02_09235"
     CDS             <1..381
                     /locus_tag="VFR02_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET9870663.1"
                     /translation="LPSHLTASGRAILAALPKSQVRALYPNAAAFSSRTEVPDPIGKY
                     STLSAHLEQVRQRGYSTENGEITEGFGSVAAAVTDHLGWPVAAVAVTFLAAKVPPGEW
                     PALAQRVTNVAGELSTRIYGRREG"
BASE COUNT          180 a          374 c          334 g          173 t
ORIGIN      
        1 ctccccagcc acctcaccgc cagcggccgc gccattttgg cggcactgcc gaagtcgcag
       61 gtccgggccc tgtatcccaa tgcggcagcg ttcagctcgc gcaccgaagt tccggacccc
      121 attggcaagt actccacctt gtccgcccac ctggagcagg tccgccagcg cggctattcc
      181 accgaaaacg gcgagatcac ggaagggttt ggctccgtgg ctgccgctgt cacggaccat
      241 ctgggctggc cggtggccgc cgtggccgtg acgttcctgg ccgcgaaggt gccccctggg
      301 gagtggccgg ccctggccca gcgcgtcacc aacgtggcgg gcgagctgtc cacccggatc
      361 tacggccgcc gcgagggctg aatttgccca gccctcccca ccgacgctcc tgcagatccg
      421 ggggtgattt tggggatgct cctgcagctt ggggtagagg gttgggggca cttccacccg
      481 gacgctcctg cagcttggcg agggtgtttt tgcggacgca cctgcagtag cggagggagg
      541 gcctgccgcc ggccgaactt gtctgcacca gatccgttcg agttgcagca cccgccccga
      601 ccatactcgg ccacgttatc aggatcgatg gcgcagctcc tgcgggcaaa ccgggcccga
      661 ggccaccata tgtgcaccgc cgattctgca cggcgggcaa tgccttccaa ccaccggacg
      721 tcgaccgggt ggtgacgcca ggccgccctc gtgccgcccc gcctttggtt gtcgaaggac
      781 gtccatgcgg ccatctgcag gagcgtcgtg tgaagcagcc cccggatctg caggagcgtc
      841 cgctggggag tctggaatcc cggacacagc gcctgtcagg aggcagaaat tcgcaccgtt
      901 aagcgccata gtggaaacta agaccgccac aacccaaagg atccaccatg accgccgctg
      961 tcctgtccca agaagtcaca ctctcctcct cgggtgtcac ccccgaggac gtgatcgccg
     1021 tcgcccgcca caacgccaag gtgaccatca gcgaagaggc g
//
LOCUS       DASTJJ010000764         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_104404, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000764 DASTJJ010000000
VERSION     DASTJJ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104404"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..>1073)
                     /gene="rpoD"
                     /locus_tag="VFR02_09240"
     CDS             complement(64..>1073)
                     /gene="rpoD"
                     /locus_tag="VFR02_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET9870664.1"
                     /translation="EKIEEQEHAMHVQEKQMKLSPEAYIKLPSLLKHATPADLKHLEK
                     KSALKGEEFHKVCAHWEEARRHIKKLEKEGMASRDQMKVMMKSIRVGEREAYHARMEL
                     VEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMRAVEKFEYRRGYKFSTYATWWIRQ
                     AITRAIADQARTIRIPVHMIETINKSIKVSRDLVQELGREPLADEIAERLEMPVEKVR
                     AILKIAQEPISLETPIGEEKDSHLGDFIEDKDVISPANAAAFVLLQEQISKVLGSLKD
                     REAEVLRLRFGLNDGYPHTLEEVGNTFKVTRERVRQIEAKALRKLRHPSRSRKLKGYL
                     D"
BASE COUNT          148 a          328 c          350 g          247 t
ORIGIN      
        1 aggggattta aaagggctta aacaaaaaag gccggcccga agggccggcc ttgggggtct
       61 cccctagtcc aggtaaccct tcagcttgcg gctgcgcgag ggatgccgca gtttccgcag
      121 ggccttcgct tctatctgcc gcacgcgctc gcgggtcacc ttgaaggtgt tgcccacctc
      181 ttccagggtg tggggatagc cgtcgttgag gccgaaacgc aggcgcagca cttccgcctc
      241 gcggtccttc aggctgccca gcaccttcga gatctgttcc tggagcagca cgaaggccgc
      301 cgcgttggcg gggctgatga cgtccttgtc ctcgatgaag tcgcccaggt ggctgtcctt
      361 ctcctcgccg atgggcgttt ccagcgagat gggctcctgc gcgatcttca ggatggcgcg
      421 caccttctcg acgggcattt ccaggcgctc ggcgatctcg tcggccaggg gttcccggcc
      481 cagttcctgc accaggtcgc gggagacctt gatggacttg ttgatggtct cgatcatgtg
      541 caccgggatg cggatggtgc gggcctggtc cgcgatggcg cgggtgatgg cctggcggat
      601 ccaccaggtg gcgtaggtgg agaacttgta gccgcggcgg tactcgaact tctccaccgc
      661 gcgcatgagg ccgatgttgc cttcctggat caggtcgaga aactgcaggc cgcgattggt
      721 gtatttcttg gcgatggaga tgaccaggcg caggttggcc tccaccagct ccatgcgggc
      781 gtggtaggcc tcgcgttcgc ccacccggat gctcttcatc atgaccttca tctggtcccg
      841 gctggccatg ccctcttttt ccagcttctt gatgtgccgg cgggcctcct cccagtgggc
      901 gcagaccttg tggaattcct cgcccttgag ggcgctcttc ttttccaggt gcttcaggtc
      961 ggccggggtg gcgtgcttca agaggctggg cagcttgatg taggcctcgg ggctgagctt
     1021 catctgcttt tcctgcacgt gcatggcgtg ttcctgctcc tcgatcttct cgc
//
LOCUS       DASTJJ010000765         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_228894, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000765 DASTJJ010000000
VERSION     DASTJJ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228894"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFR02_09245"
     CDS             <1..216
                     /locus_tag="VFR02_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870665.1"
                     /translation="LPAAVSPRVGGSWGNQGRTAWGDGVAIERAAEDGGPTRRIVVSW
                     LKETGVDQARGRINDARVNVKQSPLVV"
     gene            236..>1071
                     /locus_tag="VFR02_09250"
     rRNA            236..>1071
                     /locus_tag="VFR02_09250"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          205 a          272 c          423 g          171 t
ORIGIN      
        1 ctgccggcag cagtgtcgcc gcgcgtaggg gggtcctggg gcaaccaggg tcggaccgcg
       61 tggggggacg gtgttgcgat cgaacgggcg gccgaagatg gaggaccgac gcgccggatc
      121 gtcgtttcct ggttgaagga gacgggggtc gatcaggcgc gcggcaggat aaacgacgca
      181 cgcgtgaacg tgaaacaaag tcctctcgtg gtgtgaagcc agaggggcag acattttggt
      241 ggagagtttg atcctggctc aggattaacg ctggcggcgt gcctaatgca tgcaagtcga
      301 acgtggtctc tttggagaca aacgtggcgg acgggtgagg aacacgtggg gaacctgccc
      361 attggtgggg ggtagcccgc ggaaacgcgg ggtaattccg catacgctgg ccggcgggta
      421 gcgctggtca ggaaagggtc gtttacgact cgccgatgga ggggcctgcg tccgattagc
      481 ttgttggtgg ggtaatggcc taccaaggcg gtgatcggta gctggtctga gaggacggcc
      541 agccacaggg ggactgagac acggccccca ctcctacggg aggcagcagc aaggagtttt
      601 ccgcaatggg cgcaagcctg acggagcaac gccgcgtgcg ggatgacgcc tttcggggtg
      661 taaacccctt ttctcgggga cgaagccagt gacggtaccc ggggaagaag gcccggctaa
      721 ctacgtgcca gcagccgcgg taatacgtag gggccgagcg ttgtcctgag ttactgggcg
      781 taaagcgcgc gcaggcggtt ctccaagccc ggcgtgaaag cccccggctc aaccggggag
      841 gggcgttggg gactggggga cttgggcccg gcaggggccg gtggaatccc cggtgtagtg
      901 gtgaaatgcg tagagatcgg ggggaacacc cgtggcgaag gcggccggct gggccgtggc
      961 cgacgctgag gcgcgaaggc gtggggagcg aacgggatta gataccccgg tagtccacgc
     1021 agtaaacgat gcagactagg cgtgggggga cttgaccccc tccgtgccgg a
//
LOCUS       DASTJJ010000766         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1449803, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000766 DASTJJ010000000
VERSION     DASTJJ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449803"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..>1150
                     /locus_tag="VFR02_09255"
     CDS             150..>1150
                     /locus_tag="VFR02_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870666.1"
                     /translation="MARSSKTPHGPQWGPPRSLIVISFFLLWVAAFSVLSYAPLGLQL
                     KGWALVLGLVIPAFLLYRLDGSAKKKTVAPVFHAPPIWLWAALAGLALFLRFWKLTSL
                     FVWPNADEGLCGAVIFQLHSHWDWRFFHSYGQAPPLLYWWGLLVLKLTRSPFLSLWLG
                     PALISALTLPLMFLFLRSWWDDATAWIGTGLAAFSFWPLLIGRVNCQGDLLPAWECLL
                     LFLLSRLPALPPARRPGAGFLLGLTAGLGFLTLTPWVAVAAWVVLLFFLPFPRSRSAR
                     EGVFFLGGLGSGLLLFSAVALSEGFGRHVAHLWAESGSGGSGRFLLGLLDYLAAVFTG
                     "
BASE COUNT          112 a          397 c          343 g          298 t
ORIGIN      
        1 accggggagg cctgccgcgg accgtgcgga tgagaatgtt ggtcttgtcc taaggtcatc
       61 ctgttattct ataggcggcg ccgtcggatc cggcatgcct ttttcatccc cgggatcccg
      121 gcccccatcc cgtcgcgccc aagggaccca tggcccgatc cagcaaaact ccccacggcc
      181 cccaatgggg accgccccgc tccttgattg tcatttcttt ttttctcctt tgggtcgccg
      241 ctttttcggt cctgagctac gcccccctcg gcctgcagct gaagggttgg gcgctggtct
      301 tgggcctggt gatcccggct ttccttctct accggttgga tgggtccgcc aagaaaaaaa
      361 cggtggcgcc ggtctttcac gcccccccca tttggctttg ggcggccctg gccggcttgg
      421 cccttttcct ccggttctgg aaattgacct ccttgttcgt gtggcccaac gcggacgagg
      481 ggctttgcgg ggcggtcatc ttccaactcc actcccattg ggattggagg tttttccaca
      541 gctacgggca ggcccctccc ctgctctact ggtggggcct gttggtcctg aagctcaccc
      601 gttccccctt cctgagcctt tggctgggcc cggccttgat ctccgccctc acccttccct
      661 tgatgttcct ttttttgagg tcctggtggg acgacgccac cgcctggatc gggaccggcc
      721 tggcggcctt cagtttttgg ccgctcctga tcggccgggt caattgccaa ggggaccttc
      781 tccccgcctg ggaatgcctt ttgcttttcc tgctgtcccg cctgccggcc cttccaccgg
      841 cccgccggcc cggcgccggt tttctgctgg gcctcacggc cggcctgggc ttcctcaccc
      901 tcacgccttg ggtggcggtg gcggcctggg tggtcctgct cttcttcctt cctttccccc
      961 ggtcccgttc ggcacgggag ggcgtttttt tcctcggggg cttggggtca gggctcctgc
     1021 ttttttcagc ggtcgccctt tcggagggtt tcggccgtca cgtggcccac ttgtgggccg
     1081 aatccgggtc cgggggatcg ggccgttttc tgctgggcct tttggactac ctggccgcgg
     1141 tcttcaccgg
//
LOCUS       DASTJJ010000767         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_472731, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000767 DASTJJ010000000
VERSION     DASTJJ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472731"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1409
                     /locus_tag="VFR02_09260"
     CDS             <1..>1409
                     /locus_tag="VFR02_09260"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9870667.1"
                     /translation="GAETDLDLGHYERFLSGKLTRNSSVTSGKIYNAVITKERKGDYL
                     GKTVQVVPHVTNEIKESILKISQDQKIDVCIVEVGGTVGDIESLPFLEAIRQFKFDVG
                     KENALNIHVTLVPYIDASGELKTKPTQKTVAELRAIGIQADLILCRTARPLGEDLKGK
                     ISLFCNVEPEAVFEALDANSIYEVPLLFSEQGLDDYLVKKLALKAKPQANLGQWGRFV
                     QQVYDPRKEIEIAIAGKYVELHDAYKSIIESFIHAGVPNQAKVRLRWVDSEEVERKGA
                     AALLKGVSGVLVPGGFGDRGIEGKIEVIRHAREKKVPFFGICLGMQCAVIEFSRNVLG
                     LKQANSGEFNPKTPDEVISMMEDQLKIQGLGGTMRLGAYPCKVEKGTKAFQAYKQGLI
                     SERHRHRYEFNNAYREKVKAKGMKLSGLSPDGNLVEMIELSNHPWFVGVQFHPELKSR
                     PLNPHPLFRDFVKAAVHYK"
BASE COUNT          297 a          466 c          437 g          209 t
ORIGIN      
        1 acggcgccga gaccgacctg gacctggggc attacgaacg cttcctgtcc ggcaagctca
       61 cccgcaacag cagcgtgacc agcggcaaga tctacaacgc ggtcatcacc aaagaacgca
      121 agggcgacta cctgggcaag accgtgcagg tggtgcccca cgtcaccaac gagatcaagg
      181 aatccatcct caagatcagc caggaccaga agatcgacgt gtgcatcgtg gaggtcgggg
      241 gcacggtggg cgacatcgag agcctgccct tcctggaggc catccgccag ttcaagttcg
      301 acgtgggcaa ggagaacgcc ctcaacatcc acgtgaccct ggtgccctac atcgacgcct
      361 cgggcgagct caagaccaag cccacccaga agaccgtggc ggaactccgg gccatcggca
      421 tccaggccga cctcatcctc tgccgcacgg cccggcccct gggcgaggac ctgaagggca
      481 agatctcgct cttctgcaac gtggagcccg aggccgtctt cgaggcgttg gacgccaact
      541 ccatctacga ggtgcccctg ctcttcagcg agcagggcct ggacgactac ctggtgaaga
      601 agctggcctt gaaggccaag ccccaggcca acctgggcca atggggccgt ttcgtgcagc
      661 aggtctacga cccccgcaaa gaaatcgaga tcgccatcgc gggcaagtac gtggaactgc
      721 acgacgccta caagtccatc atcgagtcct tcatccacgc gggcgtcccc aaccaggcca
      781 aggtccgcct gcgctgggtg gattccgagg aggtggaacg caagggcgcg gccgcgctcc
      841 tgaagggcgt gtcgggcgtg ctggtgcccg gcggcttcgg ggaccggggc atcgagggca
      901 agatcgaggt catccgccac gcgcgggaaa agaaggtccc cttcttcggc atctgcctgg
      961 gcatgcagtg cgcggtcatc gagttctcgc gcaacgtgct gggcctcaag caggccaaca
     1021 gcggcgagtt caaccccaag acgcccgacg aggtcatttc catgatggag gaccagctga
     1081 agatccaggg cctgggcggc accatgcgcc tgggggccta tccctgcaag gtcgagaagg
     1141 gcaccaaggc cttccaggcc tacaagcagg gcctcatcag cgagcgccac cgacaccgct
     1201 acgagttcaa caacgcctac cgggagaagg tcaaggccaa gggcatgaag ctgtccggcc
     1261 tgtcgcccga cggcaacctg gtggagatga tcgagctttc caaccatccc tggttcgtgg
     1321 gcgtgcagtt ccaccccgag ctcaagagcc ggcccctgaa cccgcacccg ctcttccggg
     1381 atttcgtgaa ggccgccgtg cattacaaa
//
LOCUS       DASTJJ010000768         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_472766, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000768 DASTJJ010000000
VERSION     DASTJJ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472766"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFR02_09265"
     CDS             <1..235
                     /locus_tag="VFR02_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HET9870668.1"
                     /translation="IEGLERAAGVRDAFLFHAGTALKDGRVVTAGGRVLCVTGIGKTL
                     KEALQKAYEAAGQLRFEGAHYRRDIGWRALARK"
     gene            520..1176
                     /locus_tag="VFR02_09270"
     CDS             520..1176
                     /locus_tag="VFR02_09270"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9870669.1"
                     /translation="MSGKKAETKGLTYSQPNLLQLADVLRNGGIGIFPTDTIYDIGCS
                     VKKPDALKRIFALKARSLNQSLSILISRPEVALEIADFREKDMESLKKVWPGPWTLIT
                     KARAPLPAGAVGPDGTVALRCPDHGIALELLRQVGDTLAVSSANFSGAKSPARFDEID
                     PEVLEQVDCALDAGVCPLGGETAIARFENKKPRIIRTGCVSKDEVARIEGILSQINRD
                     "
     gene            1335..>1550
                     /locus_tag="VFR02_09275"
     CDS             1335..>1550
                     /locus_tag="VFR02_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HET9870670.1"
                     /translation="MAQKIVFVCTGNTCRSPMAEGLLKKAAAEAGLALQVASAGLAAF
                     PGLPAAPEALEACREKGVDLSGHLTSPL"
BASE COUNT          307 a          476 c          482 g          285 t
ORIGIN      
        1 gatcgagggg ttggagcggg cggccggggt cagggacgcc ttcctgttcc acgccgggac
       61 ggccttgaag gatggccggg tggtgacggc gggcggccgg gtgctgtgcg tgacgggcat
      121 cgggaagacc ttgaaggagg cccttcaaaa ggcctatgag gccgccggac agctccgctt
      181 cgagggcgcg cattaccggc gggatattgg ctggcgggct ttggcccgga aatagaactt
      241 caggactttt gagaccaagg aacttcccaa tgaacctatt tcaccaccaa gacaccaagg
      301 caccaaggac tgctaagatc gaaaaaccgg aaaatccgac cctttcttgg tgaacttggt
      361 gccttggtgg ttaaaatgtt gtcgtttttg atctttgttg gtgttcctaa gattcatgct
      421 gaaaaattgc ctattctctg agaaaatcaa ggaaggggcc ggttttcggc ccagttccgt
      481 cgcgggcgaa aaacggtcaa attccaacgg ggtccaacca tgagcggcaa aaaagccgag
      541 accaagggcc taacttattc ccagcccaac ctgctgcagt tggcggatgt gctgcgcaac
      601 ggcggcatcg gcattttccc cacggacacc atttacgaca tcggctgctc ggtcaagaag
      661 cccgacgccc tcaagcgcat cttcgccctg aaggcccgca gcctgaacca gtccctgtcc
      721 atcctcattt cccggcctga agtggccctc gagatcgccg atttccgcga aaaagacatg
      781 gagagcctca agaaggtctg gcccggcccc tggaccctca tcaccaaggc ccgggcgccc
      841 ctgcccgcgg gggccgtggg ccccgacggc accgtggccc tgcgctgccc agaccacggc
      901 atcgccctgg agctgctgcg tcaagtgggg gacaccctgg cggtctccag cgccaatttc
      961 tcgggcgcca agagcccggc ccggttcgac gagatcgacc ccgaggtcct ggaacaggtg
     1021 gattgcgccc tggacgcagg ggtctgcccg ctgggcgggg agaccgccat cgcccgtttt
     1081 gagaacaaaa agccccgcat catccgcacc ggatgcgttt ccaaggacga ggtcgcccgc
     1141 atcgagggga tcctgtccca gatcaaccgc gactaaagac cgtccgaaat caacggcatt
     1201 tgaaccacca agggcccaag tccaccagga aaggccggat ttaaagcctc tttccgggtc
     1261 cgggtttttc ttgatgtcct tcgtggccga acttttcgtg ggttttaaat cctgccggtc
     1321 cgggaaagcg ccagttggct caaaagatcg tctttgtctg cacggggaac acttgccgca
     1381 gccccatggc ggagggcctg ttgaaaaagg ccgccgccga ggcgggcctg gcccttcagg
     1441 tggcatcggc cggtttggcg gcctttcccg ggctgcccgc ggcgcccgag gcactggagg
     1501 cctgccggga aaagggggtg gacctctcgg ggcacctgac gagccccctg
//
LOCUS       DASTJJ010000769         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1328396, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000769 DASTJJ010000000
VERSION     DASTJJ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328396"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFR02_09280"
     CDS             <1..185
                     /locus_tag="VFR02_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870671.1"
                     /translation="LLGAHGILLFLVYATGILPVPDGGTWASKIALLSNQLALSSNYA
                     IRKAKRNPSLNNDERR"
     gene            complement(333..725)
                     /locus_tag="VFR02_09285"
     CDS             complement(333..725)
                     /locus_tag="VFR02_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870672.1"
                     /translation="MRKFTVWALAAVFVVLAGKVWAEGASKTVTLKGTLVDMACYFDD
                     GDTGDDHMGMKGCGKACLKLGSPAGLLVDGQVYTLLFPSANFADYVGKTLEITGDLYG
                     TDALIPAKAAWVDGDSKKPIKWSVKPMM"
     gene            complement(806..1282)
                     /locus_tag="VFR02_09290"
     CDS             complement(806..1282)
                     /locus_tag="VFR02_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9870673.1"
                     /translation="MTAGDLVRDLGLLPHPEGGFYRETYRAAGTTPTASGGRAHSTCI
                     YYLLVRGAKSRLHRLASDEVFHFYLGDPVTWVLLKPEGGSEEKTLGPRLDRGQSPQMV
                     VSAGTWFGGELAPGGSFALMGTTVAPGFDFADFELADPEALARDFPAAQGWIARLA"
     gene            complement(1288..1650)
                     /locus_tag="VFR02_09295"
     CDS             complement(1288..1650)
                     /locus_tag="VFR02_09295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9870674.1"
                     /translation="MTDSSDAKVVTTASGLQYQVLQPGDGPEAKPGQQVSVHYTGWLT
                     NGTKFDSSLDRGQPFQFTLGAGQVIKGWDEGVAGMKVGEKRKLTIPAALGYGPQGAGG
                     VIPPNATLIFEVQLLGVK"
     gene            complement(1909..2139)
                     /locus_tag="VFR02_09300"
     CDS             complement(1909..2139)
                     /locus_tag="VFR02_09300"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9870675.1"
                     /translation="MDVPYASDPKDVELESLIARRIRPFCSNVHVSAKGGHVTISGLV
                     EDFEDKRSAYHAALEVAGVHDVTNNIRVAATS"
BASE COUNT          441 a          743 c          673 g          415 t
ORIGIN      
        1 cgctcttggg tgctcatggc atcctccttt ttctggtgta cgcaaccggt attttaccgg
       61 taccagacgg gggtacgtgg gcatctaaga ttgcccttct gtccaatcaa ctcgcactaa
      121 gttcaaacta tgcgatcaga aaagcaaaac gaaacccatc tttaaacaat gatgaacgcc
      181 gatgaaggct gatgtcgatg ggaaaaagat gaaaaatcgg ccgttatcag cctccatcaa
      241 tgttccaaat tgtctttgct ttgcgaagtg gaccactcca aatgaaaaag gcccccgccg
      301 ggggcctttc ggacggttgg ggaaccatcc cttcacatca tgggcttcac ggaccacttg
      361 atgggcttct tggaatcccc gtccacccaa gcggccttcg cgggaatgag cgcgtcggtc
      421 ccgtaaaggt ccccggtgat ctccagggtc ttgcccacgt agtcggcgaa attggccgag
      481 gggaacagca gggtatagac ctggccgtcc accaaaagac ccgccgggct gcccagtttc
      541 aagcaggcct tgccgcaacc cttcataccc atgtggtcgt cgccggtgtc gccgtcgtcg
      601 aaatagcagg ccatgtcgac caaggtcccc ttcaaggtca ccgtcttgga ggcgccttcc
      661 gcccaaacct tgccggccag gacgacgaaa accgccgcga gggcccaaac cgtgaatttg
      721 cgcataacag cctcctgatg ttccaaggat gaagttcaga cgttgatgga tagggccgga
      781 gggtcggcca agtttcagga aatcctcagg ccagtcgggc gatccatccc tgggcggccg
      841 ggaaatcccg ggccaaggcc tccggatcgg ccagctcgaa gtcggcgaaa tcgaacccgg
      901 gcgccaccgt ggtgcccatc agggcgaaac tgccgcccgg cgccaattcc cccccgaacc
      961 aagtcccggc ggacaccacc atctgcggcg attgaccccg gtccagtcgg ggccccaggg
     1021 tcttttcttc cgacccgcct tccggcttca gcaagaccca ggtcaccggg tcccccaggt
     1081 agaagtggaa gacctcatcg gaggccaagc ggtggagccg ggacttggcg ccgcgcacca
     1141 gcaggtaata gatgcaggtc gaatgggccc gcccgcccga cgcggtgggc gtcgttcccg
     1201 cggcccggta ggtttcccgg tagaacccgc cctccggatg gggcaaaagc cccaggtcgc
     1261 ggacgaggtc cccggccgtc atggggctta tttcacgccc aacagctgga cctcgaagat
     1321 gagcgtggcg ttggggggaa tgacgccgcc ggccccttgg gggccgtagc ccagcgcggc
     1381 ggggatggta agcttgcgct tttcccccac cttcatgccg gccacaccct catcccaacc
     1441 cttgatgacc tgccccgcgc ccagggtgaa ttggaacggc tggccccggt cgagcgaact
     1501 gtcgaacttg gtgccgttgg tcagccaacc cgtgtaatgc acgctcacct gctggcccgg
     1561 tttggcctcg gggccgtcgc ccggctggag gacctgatac tgcaggccgg aagccgtggt
     1621 caccaccttg gcatcggaac tgtcggtcat cttcatccct cccgcgtttt gatcctgccc
     1681 cgccttcgaa tgacaggcgg gaagccccag gcttccgacc agggccatcg ccaaaaagaa
     1741 acggttcatc cgcatgaaaa cctccccatg ggtctcgcgt ccacggctgg cggaaagtat
     1801 acaagaggga cccggttttt ttaaccactt gtccgggcgg cgacaaaaaa gcccccgccc
     1861 gggccggggc ccgggggagg cttggaaagg gccggtgcgg cgcggggctt aggaggtggc
     1921 cgccacccgg atgttgttgg tcacgtcgtg caccccggcc acttccaagg cggcgtgata
     1981 ggcgctgcgc ttgtcctcaa aatcctcgac gagccccgag atcgtgacgt ggccgccctt
     2041 ggcgctcacg tgcacgttcg agcaaaaggg gcgtatccgc cgggcgatga gggactccaa
     2101 ctccacgtcc ttcgggtccg aagcgtatgg aacatccata aagtcctcct tccgtcggct
     2161 cctacacctt cagcatacgc cccccgggcc gccatttcaa aggtcctcga ttaacccggc
     2221 gtgacgttga cagccgcccg cggccttcct taagatgctg gccttaccca tg
//
LOCUS       DASTJJ010000770         1350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_229540, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000770 DASTJJ010000000
VERSION     DASTJJ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229540"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="mnmE"
                     /locus_tag="VFR02_09305"
     CDS             complement(<1..1051)
                     /gene="mnmE"
                     /locus_tag="VFR02_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET9870676.1"
                     /translation="MYQPGDTICAVATPPGIGGVGILRLSGPRALPAAGAFLSSPGPL
                     EKKRALLFRRLVDPRTDEVLDEGLVLVMPGPESYTGEDVVEFQLHGSPSLLSHLMELL
                     VREGLRPAEPGEFTYRAFQAGRLDLTQAEAVEALVSAQGEAARRQALRHLTGGLAAHL
                     EPLEEALKALYLKVEARLEFSEEGIPPLDLEKFKSGTERVKAALEELLESYRQGKLLR
                     DGLAIALVGPPNVGKSSLLNRLLGAERAIVTPEPGTTRDVIEGQVLMKGVPLRFFDTA
                     GLRESGDLIEAEGIRRSRLVLEEADFIFWLVDASNPKDSLAELRRQSPDPARLWVLFN
                     KEDLVRTLKVEEALGG"
BASE COUNT          229 a          480 c          443 g          198 t
ORIGIN      
        1 ggccccccag ggcttcttcg accttcaagg tgcggaccaa gtcctcttta ttaaagagca
       61 cccagagacg cgccggatca ggggattgac ggcgcagttc cgccaaggag tctttcgggt
      121 tcgacgcgtc caccagccag aaaatgaaat ccgcctcctc caggaccagg cggctgcgcc
      181 ggatgccctc cgcctcgatc aggtccccgg attcccgcag gcccgccgtg tcgaagaacc
      241 gcaggggcac acccttcatc aagacctgtc cctcgatgac gtcccgggtg gtgcccggct
      301 cgggggtcac gatggcccgt tcggccccca acaggcggtt caagaggctg gacttgccca
      361 cgttgggcgg gcccaccaac gcgatggcca ggccgtccct caagagcttg ccctgccggt
      421 agctttccaa aagctcctcc aaggcggcct tcacccgttc ggttccggac ttgaactttt
      481 caaggtcgag cggcgggatg ccctcctcgg aaaattccaa ccgggcctcg accttcaagt
      541 agagcgcctt gagcgcttcc tccaagggtt ccaaatgggc ggccaggccg cccgtgaggt
      601 gccgcagggc ctgccgccgg gccgcttccc cctgggccga caccaaggcc tccacggctt
      661 ccgcctgggt gaggtccagc ctcccggcct ggaaagcccg gtaggtgaac tcgccgggct
      721 cggcggggcg caggccttcc cggaccaaga gctccatcag gtgggaaagc agggaagggc
      781 tgccatgcag ttggaactcc accacgtcct cgccggtgta gctctcgggc ccgggcatga
      841 ccagcaccaa cccctcgtcc aggacctcgt ccgtgcgggg atccaccagc cggcggaaga
      901 gcagggcgcg ctttttttca agcgggcccg gggaggacaa aaaggccccg gccgccggaa
      961 gcgcccgggg gcccgaaagc cgcaggatgc ccaccccgcc gatccccggc ggggtggcga
     1021 cagcgcagat ggtatcgccc ggttggtaca tgcggcctcc tcaagaaatg aacgcccggg
     1081 gattatcgcc caaccccgcc ccggcgccaa ccgggacccc cggaaaacaa aaaagggccc
     1141 cggaagaccg gggcccttcg tgcggccaag cttgtctcag cggttgccga tggaatcatc
     1201 cggcgcgggc ggctcgggcg gcaccaggtt gcccaccgaa tcggcggccg cgatggcctc
     1261 cggcgagtag ggcaacggcg cttccggggc gacccggttg ccgggggcgg cttccgcctc
     1321 gggggatccc tcggcggatc cctcggcggg
//
LOCUS       DASTJJ010000771         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_350437, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000771 DASTJJ010000000
VERSION     DASTJJ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350437"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFR02_09310"
     CDS             <1..1155
                     /locus_tag="VFR02_09310"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9870677.1"
                     /translation="PTVPKKTSDDRLKIGIDARLAYRRGVGTYVANLIRALLVVDPRN
                     QYFLFNAPDFFKDQFGSSRVQWIDLASPNAAFYEQWSLPRAARRLGLDFLHYIDNSAS
                     VLSSFPYLLTLHDVMFARPLSAVRPHPTLRQNLVHWYKRMVIPPSVMKARRILTVSEH
                     SKEQIFARLGVSPHRIHVTPEGVDRAFFAPRRRPAPGKEWKVLIHGAADERKNVYRVF
                     QAAWRWMKRGRRFQLSILGMDEAELRCTDYVEQAELLGISPHLRWLGRVPREQLAGIY
                     AQADLFLYPSLLEGFGLPLLEAFACGVPVVTSNTTALPEVAGNAALLVDPESPEAIAQ
                     GVERVMTRPALRRQLVARGMRRAREFNWARTARLTLEAYEQLGDELGRNA"
     gene            1175..>1624
                     /locus_tag="VFR02_09315"
     CDS             1175..>1624
                     /locus_tag="VFR02_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870678.1"
                     /translation="MKKINAARQLKGSALVLFLSALAGLEFFGMGVAAGLRGLPVSLT
                     AVGEILLLLWLALAALEMALARALGGGEPRLAPLALGLCHSPFLLGFALPLALRLPLP
                     APHDFAWRKIQILFGVMWVSHLFLYTAWLLWKGRKGSRPVSSSFLAAA"
BASE COUNT          232 a          563 c          525 g          304 t
ORIGIN      
        1 ccgaccgtgc cgaaaaaaac aagcgatgac cgcctgaaga tcggcatcga cgcccggctc
       61 gcctaccgcc ggggggtggg aacctacgtc gccaacctga tccgcgccct gttggtcgtc
      121 gatccccgca accagtattt cctcttcaac gccccggact ttttcaagga ccaattcggg
      181 tccagccgcg tccaatggat cgacctggct tcccccaacg cggcctttta tgagcagtgg
      241 tccctgccga gggcggcccg ccggttgggc ctggatttcc tgcattacat cgacaactcg
      301 gcctccgtgc tttcgtcctt cccctatctg ttgaccctgc atgacgtgat gttcgcccgg
      361 cccctctcgg cggtgcggcc ccaccccacc ctacgccaga acctggtgca ttggtataaa
      421 cggatggtca ttccgccctc ggtgatgaag gcccggcgga tcctgacggt gtccgagcat
      481 tccaaagaac agatcttcgc ccggctgggc gtttcccccc accgcatcca cgtgaccccc
      541 gagggggtgg accgcgcctt cttcgcgccc cggcggcgcc cggccccggg gaaagaatgg
      601 aaggtcctca tccacggcgc ggccgacgag cgcaagaacg tgtaccgggt cttccaggcc
      661 gcctggcgtt ggatgaagcg gggcaggcgc ttccagctct ccatcctggg catggacgag
      721 gccgaactgc gctgcaccga ctacgtggaa caggccgagt tgctggggat ctccccccac
      781 ctgcggtggc tgggccgggt gccccgggaa cagctggccg gcatctacgc gcaggccgac
      841 ctgttccttt atccttcctt gttggagggt ttcgggctgc ccctgctgga ggccttcgcc
      901 tgcggggtgc ccgtggtgac ctcgaacacc acggccctgc ccgaagtggc ggggaacgcc
      961 gccctgctgg tggaccccga aagccccgag gccatcgccc agggggtgga acgggtgatg
     1021 acccgcccgg ccctgcgccg gcagttggtg gcccggggca tgcgccgcgc ccgggaattc
     1081 aactgggcca ggaccgcccg gctcaccctg gaagcctatg agcaactggg cgacgagttg
     1141 ggccggaacg cttgaacctt tccccgagga aaaattgaaa aagatcaacg ccgcccggca
     1201 actgaagggt tcggccctgg tactgttcct gtcggccctg gcggggctcg aatttttcgg
     1261 catgggggtg gccgcggggc ttcgcggact cccggtatcg ctcacggccg tgggggagat
     1321 cctgctcctg ctgtggctgg cgctggcggc cttggagatg gccctggcca gggccctggg
     1381 gggcggcgag ccccgcttgg cccccctcgc cttgggactg tgccacagcc ccttcctgct
     1441 ggggttcgcc ctgcccctgg cgctgcgcct gcccttgccc gcgccgcatg atttcgcctg
     1501 gcggaagatc cagatcctct tcggggtgat gtgggtttcg cacctgttcc tttacaccgc
     1561 ctggttgttg tggaaagggc gcaaagggtc ccgcccggtc tcctcttctt tcctggcggc
     1621 ggcg
//
LOCUS       DASTJJ010000772         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1573858, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000772 DASTJJ010000000
VERSION     DASTJJ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573858"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFR02_09320"
     CDS             <1..999
                     /locus_tag="VFR02_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9870679.1"
                     /translation="KKLPVFPADEKGIATRSASGKVINALAKEVPFFYGGSADLAGSN
                     DTLIEGGGDFEAGNYKGRVFHFGVREHGMGAELNGMALHGGIIPFGGTFLMFNDYMKP
                     AYRLAHLMGVQSIFVYTHDSIGQGEDGPTHQPVETLAAMRATPNGCVIRPADANESTY
                     AWLAALSRKNAPTGLVFTRQNVPTFDRSKMGAASGTLKGAYVLSEAKGGKPQVILMGT
                     GSEVQLCVKAQEILEKEGVAARVVSFPSWELFEAQSAQYQEEVLPASVRARVAVEAAT
                     SFGWHRWTGPHGKFVTLERFGASAPGAVAFKNLGFTPEKVAEAAKASLAEALAPVR"
     gene            1336..>1756
                     /locus_tag="VFR02_09325"
     CDS             1336..>1756
                     /locus_tag="VFR02_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870680.1"
                     /translation="MLCPNCKQEKWNDPVCPGCGLDPKAAFAALGGRCLAEDRPLEAA
                     RAYEALLRLDPEDWDALRQRAIAWYAEAQAVRDPVLFDQADRALAQALEKEWGWEQGH
                     QFRVNLAYTFTRLTDLRRQYQAQAQVPARAEQADRVLK"
BASE COUNT          308 a          607 c          547 g          294 t
ORIGIN      
        1 aagaagctgc cggtcttccc cgccgacgag aagggcatcg ccacccgctc ggcctcgggc
       61 aaggtcatca acgcgctggc caaggaagtg cctttcttct acggcggctc ggcggacctg
      121 gcgggttcca acgacaccct gatcgagggc ggcggcgact tcgaggccgg caactacaag
      181 ggccgggtct tccatttcgg cgtgcgcgag cacggcatgg gggccgagct caacggcatg
      241 gccctgcacg gcgggatcat ccccttcggc ggcaccttcc tcatgttcaa cgactacatg
      301 aagcccgcct accgcctggc ccacctgatg ggcgtgcagt ccatcttcgt ctacacgcac
      361 gattccatcg ggcagggcga ggacggcccc acccaccagc ccgtcgagac cctggccgcc
      421 atgcgcgcca cccccaacgg ctgcgtgatc cgacccgcgg acgccaatga atccacctac
      481 gcctggctgg ccgcgctctc gcgcaagaac gcccccacgg gcctggtctt cacccgccag
      541 aacgtgccca ccttcgaccg ctccaagatg ggcgcggcgt ccggcacctt gaagggcgcc
      601 tacgtgctgt cggaggccaa gggcggcaag ccccaggtga tcctgatggg cacggggtcc
      661 gaggtgcagc tttgcgtgaa ggcccaagag atcctggaga aggaaggcgt ggcggcccgc
      721 gtggtcagct tcccttcctg ggaactcttc gaggcccaga gcgcccaata ccaggaagag
      781 gtgctgcccg cctcggtgag ggcccgcgtg gccgtcgagg ccgccacctc cttcggctgg
      841 caccgctgga ccggccccca cggcaagttc gtgacgctgg agcgtttcgg cgccagcgcc
      901 cccggcgccg tggccttcaa gaacctgggc ttcacgcccg agaaggtcgc ggaagccgcc
      961 aaggcgtccc tggccgaggc cctggcgccc gtgcgctgag cctgaagccg tcctaagccc
     1021 ccggcacgaa cgtgccgggg gcttttttat tcgggattat gcgtttcgca aagcaaggac
     1081 aattggaaca ctgataaaag ctgataaagc ctgattttat tttcaaaaat aagatttcat
     1141 cagtttttat cattgtttca agttctcttt ggcttgcttt gcaaaagcca tagttccgtt
     1201 tttatttaaa ggtctttgga tgcccggccc acccgggtaa ctccttgaag gaagacgggc
     1261 ggcgggggct ttttttatgg gccgcttccc ctacaataag gcccatcgcc gaagatcctc
     1321 gcgcccttgg aaaccatgct ctgccccaac tgcaaacaag agaaatggaa cgatccggtc
     1381 tgcccgggct gcggcctgga cccgaaggcc gccttcgcgg ccctgggcgg ccgctgcctg
     1441 gcggaagacc gcccgctcga ggccgcccgg gcctatgagg ccctcctgcg cctggacccg
     1501 gaggactggg acgccctccg ccagcgcgcc atcgcctggt acgcggaggc ccaggccgtc
     1561 cgggaccccg tcctgttcga ccaggccgac cgggccctgg cccaggccct ggagaaggaa
     1621 tggggttggg agcagggcca tcagttccgc gtcaacctgg cctatacctt cacccgcctg
     1681 acggacctgc gccggcaata ccaggcccaa gcgcaggtcc ccgcccgggc cgagcaggcc
     1741 gaccgggtac tgaagg
//
LOCUS       DASTJJ010000773         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_963419, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000773 DASTJJ010000000
VERSION     DASTJJ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963419"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1668
                     /locus_tag="VFR02_09330"
     CDS             <1..>1668
                     /locus_tag="VFR02_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870681.1"
                     /translation="EVWTPNPTPVLSWEPPEDLSGVKGYFVKVDREPGTLPGPTVGDF
                     TEATRLTLGPLEDGLWYAHVSTQDKAGNLGQQAAHYALRIDTQALPPGIASPSHPKPG
                     EWYRSKDLQVELTEPHELSGVEGYYFTLDQDPTALPNPDGSQWTTKARFTVHDVEDGA
                     WFLHVRTKDQAGNLSNQASHFKVCVDTTAQPPLLSSPSHPEPQRWYAQRKVRLQWEDP
                     LDHSGVEGYFFLLDKRPDTLPIRQNATFTDKREIVLEVSDDGQWYFHLATQDKAGNLD
                     AKGAHYSLKVDTTAGTPLIRSVSHPDEGRWYAKTRVSFKFHPPADPSGITGYYYTFSE
                     DEKALPDPKTSAFTDQTELELDAPRDGVHVLSLICQDAAGNVSAQPARCRVKVDTQAL
                     APELASGSHPDPQRWYAARRVELSWKEPADLSGIEGYYFAVSPQEDFTVLPEKMTWIP
                     GTHTTFTLPEDGTWYAQVAAKDKAGNLGPAGRLKLQVDSMALPPRVTSPTHPAHHWVK
                     NAVPQFTWEDPAELSGVEGYYLVLDGKPGTLPTPEAAQWVKGNQFTAP"
BASE COUNT          292 a          630 c          514 g          232 t
ORIGIN      
        1 ggaggtctgg acgcccaacc ccacgcccgt gctgtcctgg gaaccgcccg aggacctgtc
       61 gggggtgaag ggctatttcg tgaaggtgga ccgggaaccg ggcaccttgc ccggccccac
      121 ggtgggcgac ttcaccgagg ccacccgcct gaccctgggc ccgttggagg acggcctctg
      181 gtacgcccac gtgtccaccc aggacaaggc cggcaacctg ggccagcagg ccgcccatta
      241 cgccctgcgc atcgacaccc aggcgctccc cccgggcatc gcgtcgcctt cccaccccaa
      301 acccggggaa tggtaccggt cgaaggacct gcaggtggaa ctcaccgaac cccacgagct
      361 ttcgggcgtg gagggttatt atttcaccct ggaccaggac cccaccgccc tgccgaaccc
      421 cgacggctcc caatggacga ccaaggcccg tttcacggtg cacgacgtgg aggacggcgc
      481 ttggttcctg cacgtgcgca ccaaggacca ggcgggcaac ctgtccaacc aggcctccca
      541 tttcaaggtc tgcgtggaca ccacggccca gccgcccctg ctctcctcgc ccagccatcc
      601 cgaaccccaa cgctggtacg cgcagcgcaa ggtgcggctg cagtgggagg acccgctgga
      661 ccattcgggc gtggagggct atttcttcct gttggacaag cgccccgaca ccctgcccat
      721 ccggcagaac gccaccttca cggacaagcg ggagatcgtc ctggaggtct cggacgacgg
      781 ccagtggtat ttccacctgg ccacccagga caaggcgggc aacctggacg ccaagggcgc
      841 ccattacagc ctgaaggtgg acaccacggc ggggaccccg ctgatccggt ccgtgagcca
      901 tcctgatgag gggcgctggt acgccaagac ccgggtctcc ttcaagttcc acccgcccgc
      961 cgacccctcg ggcatcacgg gctactacta caccttctcg gaggatgaga aggccctgcc
     1021 cgaccccaag acctccgcct tcaccgacca aaccgagctg gagctggacg cgccccggga
     1081 cggcgtgcat gtcctctccc tcatctgcca ggacgcggcg ggcaacgtct ccgcccaacc
     1141 cgcccgctgc cgcgtcaagg tggacaccca ggccctggcc ccggaattgg cctcgggcag
     1201 ccatcccgac ccccaacgct ggtacgccgc ccggcgggtg gagctttcct ggaaggaacc
     1261 cgcggacctg tcgggcatcg agggctatta cttcgccgtg tccccccagg aggatttcac
     1321 cgtcctgccc gagaagatga cctggatccc gggcacccac accaccttca ccctgcccga
     1381 ggacgggacc tggtacgccc aggtggcggc caaggacaag gcgggcaacc tgggcccggc
     1441 gggccggctc aagctgcagg tcgactccat ggccctgccg ccccgggtga ccagccccac
     1501 ccacccggcc caccattggg tgaagaacgc cgtgccccaa ttcacctggg aggacccggc
     1561 ggagctttcg ggcgtggagg gctattacct ggtcctggac ggcaagcccg gcaccctgcc
     1621 cacgcccgag gcggcccagt gggtgaaggg caaccagttc accgcgcc
//
LOCUS       DASTJJ010000774         1111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_593810, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000774 DASTJJ010000000
VERSION     DASTJJ010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593810"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFR02_09335"
     CDS             <1..123
                     /locus_tag="VFR02_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9870682.1"
                     /translation="FDGAVVAEISTRKAKGAGQREEWLAETLEFARVHLGQTIA"
     gene            848..>1111
                     /locus_tag="VFR02_09340"
     CDS             848..>1111
                     /locus_tag="VFR02_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9870683.1"
                     /translation="MSKKIVFLGCGSMGEAILTGLLAAGTTPQSVTVTVRRPERAEEL
                     AQAHGVTALASNEEGNANVEAVAGADVVILGVKPVGIAALCREV"
BASE COUNT          217 a          323 c          385 g          186 t
ORIGIN      
        1 tttgacggcg ccgtggtggc cgagatttcc acccggaagg ccaagggcgc cggccagcgc
       61 gaggaatggc tggccgaaac cctcgaattc gcccgcgtgc acctgggcca gaccatcgcc
      121 tgacagcccg gagccggcca accggacccc acgccttaac aacgctcggt tgcaatcggg
      181 ggtgtttggg gcaacgcttc cgcacccggc gcgggagcgt ggggaaaaac acccccggtt
      241 gcgcgggagc gtttgttggt tgctgcctgg gccggcgcct ggggcatggg accggggctt
      301 ggtggaccag ggcctggggc ttaacggcct ggggcttaac gcgaaatcgc cggagaacat
      361 gtggccagtg gatgggggga aacactggcg agttctccgg cgatttgact ggtcttcagg
      421 acaggaccag tccgatcatc actcgcaccc ttatgaggta cttaaaacat taggtcccgt
      481 ggttgggaaa aggccgggcg caggctgtgc atttgccgtt aagtcgggcc ggtggctaca
      541 cgtcaccaag agtcaccaaa aaccgacctg ttaacccaaa taacccggtt aacgagaaac
      601 gccggaagca atgccaggcc ttgggggaaa cctagccgct tccggcgctc cggaccggct
      661 gctgaaaaca actgccggtc cgtcatcact cgcaccttta tgaggtggtt atgacagtaa
      721 tgggcctttc tgggcaaaga ccgccacggg gctgggaaaa ggctggcagt gtcaggtccg
      781 cccgtcccgt gacggcgcga aacggcgctt tgttgcatgc ggggcgccgg ggcagaaaga
      841 tggggggatg agtaaaaaga tcgtatttct aggctgcgga tccatgggcg aggcaatttt
      901 gaccggactg ctggctgccg ggaccacgcc acagtccgtc accgtgaccg ttcgccggcc
      961 cgaacgggct gaagaactgg cacaggccca cggggtcacc gcgctggcct cgaacgagga
     1021 agggaacgcc aacgtcgagg ccgtggccgg ggccgacgtc gtgatcctcg gcgtcaagcc
     1081 cgtgggcatt gcggcgttgt gccgggaggt c
//
LOCUS       DASTJJ010000775         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_230050, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000775 DASTJJ010000000
VERSION     DASTJJ010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230050"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFR02_09345"
     CDS             <1..286
                     /locus_tag="VFR02_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870684.1"
                     /translation="APIAQVLNSGVRVDYWIDVDRSEAAKLTGLPFQASVAERGGFQV
                     PGDALDGGLAKLQAKGAKVGAVRPLASVVEAYFLKAIGRPASFDPARGAA"
     gene            283..1059
                     /locus_tag="VFR02_09350"
     CDS             283..1059
                     /locus_tag="VFR02_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870685.1"
                     /translation="MNRVYQVAQFTFRELLRSNLLIVWIVSVIAIGGLSFLLSLLSYG
                     DVLKIFTDLGLAGTELAGWLVLLLSLAVTYTTDMDQKAIFLQLAKPVTRGEYLLGRVL
                     GFFAVNLLVVLGNVAFLVFMVVVVAGGSIPPLFLPSVAFLALEMLVLTFLGLTYQMIA
                     TTMVRVVLYTFFTMLLGRMIGEIQWLISKQSAPLLKAVLTVVYYCLPNLQVFDLKDRI
                     YDPALVLGWTQWQEVLLYSFSYSAVVFLIGWIALERREFR"
     gene            1059..1772
                     /locus_tag="VFR02_09355"
     CDS             1059..1772
                     /locus_tag="VFR02_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870686.1"
                     /translation="MPAPALSFKGDWRLKALPAVLALEILFSTLLGAWVHPTPFAAER
                     LGRDVPSALRAVGYLTGFKLLVGHLFWIRVIQYYGDSDNASTRYEKLYGYCSLATDLN
                     PNFIPIYTFGAAALSFHLKRPLEAARLLQKGIEANPHEDRLKMLYAAIAYQNAGDYEK
                     VLPFLEAQVARGDAPEMLVNILANTYEEAGHLDQAIRLWKHILLNTDDQATRIEAAQR
                     LQKLYHEAQAAPAAKKPRP"
     gene            1769..>2623
                     /locus_tag="VFR02_09360"
     CDS             1769..>2623
                     /locus_tag="VFR02_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9870687.1"
                     /translation="MIHLLNANPALDFDFKLSDPASGKIGAVESFTVSPGGKALNVAR
                     FLKALRVPARLWVPSGEREATQVLYFHLARRKDLSVQWVPQEGPVRANVVLHNSGGSA
                     KFNHSGFPLSAAPWDRLLARRLRPGDLLALTGRLPAQNAGLYAGWIKTWQPKGVRVAL
                     DASGEGLRRAMAQRPWFLKVNLDEFNGAWAPACPTLTRVAAQVPKLRRKGLIHGAVTH
                     GAEGALVWEGGRVLGVKNAAVKDRKLVVGAGDGFLAGYLAAVQAKLDLADRCRWASAA
                     ARVVAEKGI"
BASE COUNT          385 a          930 c          847 g          461 t
ORIGIN      
        1 ggcccccatc gcccaggtcc tgaactccgg ggtgcgggtg gactattgga tcgacgtgga
       61 ccgctccgag gccgccaagc tcaccggcct gcccttccag gcttccgtgg ccgagcgcgg
      121 gggcttccag gtccccgggg acgccttgga cgggggcctg gccaaactgc aggccaaggg
      181 cgccaaggtg ggggccgtcc ggcccctggc gtcggtggtg gaggcctact tcctcaaggc
      241 catcggccgg ccggcctcct tcgatcccgc ccggggggcc gcatgaaccg cgtctaccag
      301 gtcgcccaat tcaccttccg cgagctgttg cgcagcaacc tgctgatcgt ctggatcgtg
      361 tcggtcatcg ccatcggcgg cctttctttc ctgctgtcgc tcttgagcta cggggacgtg
      421 ctgaagatct tcaccgacct gggcctggcc ggcaccgaac tggcgggctg gctggtgctg
      481 ctcctgagcc tggcggtcac ctacaccacc gacatggacc agaaggccat cttcctgcag
      541 ttggccaagc cggtaacccg gggcgagtac ctgctgggcc gggtgctggg tttcttcgcg
      601 gtgaacctgc tggtggtgct gggcaacgtg gccttcctgg tcttcatggt ggtcgtggtg
      661 gccggcggtt ccatcccccc gcttttcctg cccagcgtgg ccttcctggc gctggagatg
      721 ctggtcctga ccttcttagg gctcacctac cagatgatcg ccaccaccat ggtccgggtg
      781 gtgctctaca ccttcttcac catgctgctg ggccgcatga tcggggagat ccaatggctc
      841 atcagcaagc agtccgcccc cctcctcaag gccgtgctga cggtggtcta ttactgcctg
      901 cccaacctgc aggtcttcga cctgaaggac cgcatctacg accccgccct ggtgctgggc
      961 tggacccagt ggcaggaagt actgctctac agcttcagct attcggcggt ggtgttcctc
     1021 atcggctgga tcgcgttgga acgccgggag ttccgctgat gcccgccccg gccctgtcct
     1081 tcaaggggga ctggcgcctc aaggccctgc cggccgtcct ggccttggag atcctttttt
     1141 cgaccctgct gggggcctgg gtgcacccca cgcccttcgc cgccgagcgc ttgggccggg
     1201 acgtgccttc cgcgctgcgg gccgtgggct acctgacggg cttcaaactg ctggtggggc
     1261 acctcttttg gatccgggtc atccagtatt acggcgactc ggacaacgcc tccacccgtt
     1321 acgaaaagct gtacggctat tgcagcctgg ccaccgacct gaaccccaat ttcatcccca
     1381 tctacacttt cggggccgcc gccctttcct tccacctgaa gcgccccctg gaggccgccc
     1441 ggctgctgca aaagggcatc gaggccaacc cccatgagga ccggctcaag atgctctacg
     1501 ccgccatcgc ctaccagaac gcgggggatt acgagaaggt cctgcccttc ctggaggccc
     1561 aagtggcccg gggcgacgcc cccgaaatgc tggtgaacat cctggccaac acctacgagg
     1621 aggccgggca cctggaccag gccatccggc tctggaagca catcctgctg aacaccgacg
     1681 accaggccac ccgcatcgag gcggcccagc gcctgcagaa gctttatcac gaggcccagg
     1741 ccgcccccgc ggccaagaag ccccgcccat gatccacctc ctcaacgcca atcccgcgct
     1801 ggatttcgac ttcaagcttt ccgaccccgc ctcgggcaag atcggcgcgg tggaatcctt
     1861 cacggtgtcg ccggggggca aggccctcaa cgtggcgcgc ttcctcaagg ccctgcgcgt
     1921 gcccgcccgc ctctgggtgc cctcgggcga gcgggaagcc acccaagtgc tttatttcca
     1981 cctggcccgc cggaaggacc tttcggtcca atgggtgccg caggaaggcc cggtacgggc
     2041 caacgtggtg ctgcataatt cgggcggatc ggccaagttc aaccattcgg gtttcccgct
     2101 ctcggccgcg ccttgggacc ggctgctggc ccggcgcctg cgcccgggcg acctgttggc
     2161 cctcacgggc cgcctgccgg cgcagaacgc cggcctctac gccggatgga tcaagacgtg
     2221 gcaacccaag ggcgtgcggg tggccctgga cgcctcaggg gagggcctgc gccgggccat
     2281 ggcccaaagg ccctggttcc tcaaggtcaa cctggacgag ttcaacggcg cctgggcccc
     2341 cgcctgcccc acgctcaccc gggtggcggc ccaagtgccg aaactgcgcc gcaaaggcct
     2401 gatccacggc gccgtcaccc acggggccga gggggccctg gtctgggaag ggggccgggt
     2461 gctgggcgtg aagaacgccg ccgtcaagga ccgcaagctg gtcgtggggg cgggggacgg
     2521 ctttttagcg ggctacctgg cggccgtcca ggccaagctg gacctggccg accgctgccg
     2581 ctgggcctcc gccgccgccc gggtggtggc cgaaaagggc atc
//
LOCUS       DASTJJ010000776         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_350820, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000776 DASTJJ010000000
VERSION     DASTJJ010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350820"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1511
                     /gene="sufB"
                     /locus_tag="VFR02_09365"
     CDS             66..1511
                     /gene="sufB"
                     /locus_tag="VFR02_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9870688.1"
                     /translation="MKPKTQEQPIEQVVREDYKYGFVTDIEADSAPPGLNEDIIRFIS
                     AKKKEPKFLLEWRLKSYRFWTKMKEPTWPSVKYPAINYQDIIYYSAPKAGPKSLDEVD
                     PEILKTFEKLGVPMQEREALAGIAVDAVFDSVSVGTTFRAKLAEMGVIFCSFSEAVQE
                     HPDLVEKYLGSVVPYNDNFFAALNSAVFTDGSFCYIPKGVRCPMELSTYFRINAAKTG
                     QFERTLLIADADAYVSYLEGCTAPMRDENQLHAAVVELVAHDRAQIKYSTIQNWYPGD
                     KDGKGGVYNFVTKRGACRGERSKISWTQVETGSAITWKYPSCILQGDGSVGEFYSVAV
                     ANHFQQADTGTKMIHLGKDTSSTIISKGISAGHGQNTYRGLVRVQPGAEGAKNYTQCD
                     SLLMGDQCGAHTVPYIEVKNPTARVEHEASTSKIGEDQIFYCRQRGLSTEDAVNLIVN
                     GFCKEVFRKLPMEFAVEAQKLLGVSLEGSVG"
     gene            1779..2542
                     /gene="sufC"
                     /locus_tag="VFR02_09370"
                     /pseudo
     CDS             1779..2542
                     /gene="sufC"
                     /locus_tag="VFR02_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507974.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
     gene            2539..>3281
                     /locus_tag="VFR02_09375"
     CDS             2539..>3281
                     /locus_tag="VFR02_09375"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HET9870689.1"
                     /translation="MTTDLAKARESILGTIPPAGGPDWLRTLREGAFAHFKTQGFPTP
                     KNERWKYTDLSLYWPNELPGAGKPAQAAPLARLKAQGFDVEKGHWLVFIDGIHSPGQS
                     SAHPLPAGLRAGAMASMLEDPSVRETLGRIAAPESDALTALNLAAFRDGAFLQVAAGA
                     QVPGTIHLVFVSTQPAPAFARNLVLAGDNSRLSLAEHFLGDAGGPSLFSSVTELKLAP
                     GAQVDHVKIQQGSTGQLHFGTVQARQEAG"
BASE COUNT          666 a         1002 c         1075 g          538 t
ORIGIN      
        1 gagcccgaga tggacaaatg gttgaaggaa aaaaaggccg ggccggtccc ggtccaggag
       61 aaggcatgaa gccgaagacg caggaacaac ccatcgagca ggtggtccgg gaggactaca
      121 aatacggctt cgtcaccgac atcgaggccg actcggcacc gccgggcctg aacgaggaca
      181 tcatccgctt catctcggcc aagaagaagg aacccaagtt cctgctggaa tggcggctca
      241 agagctaccg cttctggacc aagatgaagg agccgacctg gcccagcgtg aaatatcccg
      301 ccatcaacta ccaggacatc atctattatt cggcgcccaa ggccgggccc aagagcctgg
      361 acgaggtgga ccccgagatc ctgaagacct tcgagaagct gggcgtgccc atgcaggagc
      421 gggaagcgct ggccggcatc gcggtggacg cggtgttcga cagcgtgtcg gtgggcacca
      481 ccttccgggc caagctggcc gagatgggcg tgatcttctg ctccttctcg gaagccgtgc
      541 aggagcaccc ggacctggtg gaaaagtacc tgggctcggt ggtgccctac aacgacaatt
      601 tcttcgcggc cctgaacagc gcggtcttca ccgacggctc cttctgctac atccccaagg
      661 gcgtgcgctg ccccatggag ctttccacct atttccgcat caacgcggcc aagacgggcc
      721 agttcgagcg gacgttgctc atcgccgacg cggacgccta cgtcagctac ctggaaggct
      781 gcacggcccc catgcgcgac gagaaccagc tgcacgccgc cgtcgtcgag ctcgtggcgc
      841 acgaccgggc gcagatcaag tattcgacga tccagaactg gtaccccggc gacaaggacg
      901 gcaagggcgg ggtctacaac ttcgtgacca agcgcggggc ctgccggggc gagcgctcga
      961 agatctcctg gacccaggtg gagacgggct cggccatcac ctggaaatat cccagctgca
     1021 tcctgcaggg cgacgggtcg gtcggggagt tctattcggt ggccgtggcc aaccacttcc
     1081 agcaggccga cacgggcacc aagatgatcc acctgggcaa ggacaccagc agcaccatca
     1141 tttccaaggg catttccgcc gggcacggcc agaacaccta ccggggcttg gtgcgggtgc
     1201 agccgggcgc tgagggcgcc aagaactaca cccagtgcga ttcgttgctc atgggggacc
     1261 agtgcggagc ccataccgtg ccctatatcg aggtcaagaa cccgacggcc cgggtggaac
     1321 acgaggcctc cacctccaag atcggcgagg accagatctt ttactgccgc cagcgcgggc
     1381 tttccacgga ggacgcggtc aacctgatcg tgaacggttt ttgcaaggaa gtgttccgga
     1441 agctgcccat ggaattcgcc gtggaggccc agaagctcct gggcgtcagc ctggaaggca
     1501 gtgtcggata aggacaacgt gatgggtttt gagccgcggg tttttgaaat tttcggtcct
     1561 ggtccgaacg ggcgctttaa ctgaggactt aaaacaaaaa cctgtttcac cgccaaggcg
     1621 ccaagagcgc caagggctgc aaaggcggaa aaccgggaaa cctggacgga tttgatcgcc
     1681 cttcttggcg gacttggcgt cttggcggtg aaaaagtagt tttcatttga tctttgttga
     1741 gagccttaaa aactgacgac tggatcgagg agactcggat gttgacgatc aagggcctgc
     1801 gggccaaagc ggaagacaaa caaatattga acggcatcga cctgaccgtg aacccggggg
     1861 aagtgcacgc catcatgggc cccaacggct cgggcaaaag caccctggcc agcgtgctgt
     1921 cgggccggga ggactacgag gtcaacgggg ggcaggccct cttcgagggc aaggacctgc
     1981 tggcgctccc ggcggaagaa cgcgcccggc tgggggtctt cctggccttc caatatccgg
     2041 tggagatccc gggggtcaac aacacctatt tcctgaaggc cgcggtcaac gcggtgcgca
     2101 aggcccgggg cctggaagaa ctggacgccg tggatttcct ggaggtggtc aaggccaagc
     2161 tcaaggaact caagatggac gagagcatgc tctcccggtc ggtcaatgag ggcttctcgg
     2221 gcggcgagaa aaaaagaacg agatcctgca gttggccgtg ctggaaccca agctggccat
     2281 cctggatgag acggactccg gcttggacat cgacgccctc aaggtggtgg ccgacggggt
     2341 caacgccttc aaacgcaagg accgggcggt cctgctggtg acccattacc agcgcctgct
     2401 ggactacatc gtgccggacc gggtgcatgt gctggccgaa ggccgcatcg tgaggtccgg
     2461 gggcaaggaa ctggccctgg aactggaaga gaagggttac ggctggatcc tggacggcct
     2521 gaagggcgcg gcccagcgat gacgaccgac ctggccaaag cccgggaatc gatcctgggg
     2581 acgatccccc ccgccggtgg gcccgattgg ctgcggaccc tgcgggaggg cgccttcgcc
     2641 cacttcaaga cccagggttt tcccacaccc aagaacgagc gctggaaata cacggacctt
     2701 tcgctttatt ggcccaacga gcttccgggc gcgggaaaac ccgcccaggc ggcgcctttg
     2761 gcccgcttga aggcgcaggg tttcgacgtg gaaaaaggcc attggctggt cttcatcgac
     2821 gggatccatt cacccggcca atcgtccgcg catccccttc cggcgggcct tcgggcgggg
     2881 gccatggcct cgatgttgga agacccctcg gtccgggaga ccctgggccg catcgccgcc
     2941 ccggaatccg acgccttgac ggcgctgaac ctggcggctt tccgggacgg cgcctttctc
     3001 caggtggcgg cgggggccca ggtccctggg accatccact tggtcttcgt ttccacccaa
     3061 ccggccccgg ccttcgcgcg caacctggtg ctggccggcg acaacagccg tctttccctg
     3121 gcggagcatt tcctggggga cgccgggggc ccctcgcttt tcagctcggt gaccgagctg
     3181 aagctggccc cgggcgcgca ggtcgaccat gtgaagatcc agcagggatc gacgggccaa
     3241 ctgcattttg ggacggtcca ggcccggcag gaagcgggca g
//
LOCUS       DASTJJ010000777         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1450671, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000777 DASTJJ010000000
VERSION     DASTJJ010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450671"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFR02_09380"
     CDS             <1..338
                     /locus_tag="VFR02_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870690.1"
                     /translation="MDRMKMKNGANDAQVMDMLNETVRLNNVLADAQFHLGKLYEQQG
                     RSQEAEKLLQAAVRMNPQRVEAQMELARLMEKTGNLPEAEEAFRGVLKADPQNKPAQT
                     ELWKLLNKP"
     gene            349..>1976
                     /locus_tag="VFR02_09385"
     CDS             349..>1976
                     /locus_tag="VFR02_09385"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9870691.1"
                     /translation="MARTGTDIPGGWGRWGAAGLFLALFQVLLRAENPDLMSDDGGEM
                     IAASWTLGLPHPPGYPLFDLVGHLFSRLPLGSPAFRYNLLSGLFALGAVLLTAGLVLR
                     LARREMGRGEAAWVPPALALASALALFFDGNFFAQALSAKGALYLSMALLAGGLAAGW
                     LAREGKPLGRREQALVLFGWSLGISAHWETAVLFLPFLGLWFWLSRRRWTSKSLLLAL
                     TAGLLGLSPYLYLPLRAALHAWPSWGAPVTWTGFRWVVGRGLVSGHESFLHDWASYVG
                     SAQRVGAVLAGGLPGAWVLVLVGWSAAWRQRWGWVGALGVLAAPVVAAVVAVREAQDI
                     HYLLGAYLVSVSVPWVLFAFAGFRSLFDRLGTGGRWMGPALLAVALLALSIWGCRVLY
                     LEGKADYSLADDLGKNALRGIPRGGLLVAGGDHYVMPVLYDQAVLGLRPDVLFVPDVF
                     LAHQWGWEQITERRPDWGEGWKGQVLGRRWEWLVSKARASGGAYYALGPRYLDPVLAQ
                     APGTWIPAGLARRWAKEDRPDEAAPSLAAMDGER"
BASE COUNT          246 a          577 c          750 g          403 t
ORIGIN      
        1 agatggaccg gatgaagatg aagaacggcg ccaatgacgc ccaggtgatg gacatgctca
       61 atgagaccgt gcggctcaac aacgtgttgg cggacgccca gttccatttg gggaaactgt
      121 atgagcagca ggggaggtcc caggaggcgg agaaactgct ccaggcggcg gtccgcatga
      181 acccccagcg ggtggaggcc cagatggaac tggcccgcct gatggaaaag accggcaacc
      241 tgcccgaggc ggaggaggcc ttccggggcg tgctgaaggc cgatccccag aacaagccgg
      301 cccagaccga attgtggaag ctgctgaata aaccctgagg ggggaccgat ggcgcggacc
      361 ggaacggata tccccggggg ctggggacgt tggggcgcgg cggggttgtt cctcgcgctc
      421 ttccaggtcc tgctccgggc cgagaatccg gacctcatgt ccgacgacgg cggggaaatg
      481 atcgcggctt cctggacctt gggcctgccc catccccccg gatatcccct cttcgacctg
      541 gtgggtcacc ttttttcccg gctgcccttg ggttcccccg ctttccgcta caacctcttg
      601 tccggccttt tcgcgctggg cgccgtgctc ctgaccgcgg gcctggtcct gcggttggcc
      661 cggcgggaaa tgggacgcgg ggaagcggcc tgggtccccc cggcgctggc cttggcgtcg
      721 gccttggcgc ttttttttga cgggaatttt ttcgcccagg ccttgagcgc caaaggggcc
      781 ctttatcttt ccatggccct gttggccggc ggcctggccg ccgggtggct ggcgagggag
      841 gggaagccct tagggcgtcg ggaacaagcg ctggtccttt ttgggtggtc cttggggatc
      901 tcggcccact gggaaacggc ggtcctgttc ttgcccttcc tgggcctgtg gttctggctc
      961 tcccggcggc gttggacctc caagtcgttg ctcttggccc tgaccgcggg gttgctgggt
     1021 ctttcacctt atttatacct tcctctgcgg gcggctttgc acgcttggcc cagttggggg
     1081 gcgccggtga cctggacggg gttccggtgg gtggtcgggc ggggattggt gagcgggcat
     1141 gaaagcttcc tgcatgattg ggcttcctac gtgggatcgg cccagcgggt gggtgctgtc
     1201 ctggccgggg gcctgccggg ggcttgggtc ctggtcctcg tgggatggtc cgccgcttgg
     1261 cgccaacggt ggggttgggt gggggccttg ggggtcctgg cggccccggt ggtggcggcc
     1321 gtggtggccg tccgggaggc gcaggacatc cattacctgt tgggggccta tttggtttcg
     1381 gtgtccgtgc cctgggtcct ttttgccttc gcggggttcc ggtcgctttt cgaccgcttg
     1441 gggaccggcg gaaggtggat ggggccggcg ctcttggcgg ttgcgctcct cgccttgtcg
     1501 atctggggat gccgcgttct ctatttggaa ggcaaggcgg attactccct ggcggatgac
     1561 ctggggaaga acgccctgcg ggggatcccg cgcggggggc tgttggtggc cgggggcgac
     1621 cattacgtga tgccggtcct ttacgatcag gcggtgctgg ggctccggcc cgacgtgctt
     1681 ttcgtccccg acgttttcct ggcccaccag tggggatggg aacagatcac cgaacggcgg
     1741 cccgattggg gcgagggttg gaaggggcag gtgttggggc gccgttggga atggctggtc
     1801 tcgaaggcgc gggcgtcggg cggcgcctat tacgccctgg gcccgcggta cctggacccg
     1861 gtcctggctc aagcgccggg gacctggatc cccgcggggt tggcccggcg ttgggccaag
     1921 gaggaccggc cggacgaagc cgccccatcg ctcgcggcca tggatggtga gcgtta
//
LOCUS       DASTJJ010000778         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_963691, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000778 DASTJJ010000000
VERSION     DASTJJ010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963691"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VFR02_09390"
     CDS             complement(<1..918)
                     /locus_tag="VFR02_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9870692.1"
                     /translation="MSASLNPHRAASIALKEVRHIVRDPFTLGLALGLPLLMVLFFGV
                     AMDFNVHDIHLTVYDQDRSRASRQLAEAFESSGYFKLRQAPLAADVTAPLDADRAKAV
                     LVIPPRFGRYLASEDGSPAQVLIDGSDNSTAGILGAYLTGIQAAASARLGGDAPPPDF
                     TLKTRYLFNPELNSRWFTVPGLSAVVLAIISILLTALTVAREWENGSMELLLATPARP
                     LEIILGKLAPYVVLGLGAQLLIYLLARFYFGIPFLGSHLVFLAGTFLFLSCYMALGLV
                     ISVATRQQMLALQVAMMVGMLPNLILSGFI"
     gene            complement(915..>1258)
                     /locus_tag="VFR02_09395"
     CDS             complement(915..>1258)
                     /locus_tag="VFR02_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9870693.1"
                     /translation="HYMDEAEQCGRIALMRDGRIIALNSPAALKKSAFPEPLWELSPQ
                     PHAPRGWARGLEKDGAVRSLSPHGLRYHAVAGAPRAWSAALRRLQGKVKVRRIPPSLE
                     DVFIRLVEGKQ"
BASE COUNT          232 a          376 c          476 g          174 t
ORIGIN      
        1 gatgaagccg gacaggatga ggttgggcag catgcccacc atcatggcca cctgcagggc
       61 cagcatctgc tggcgggtgg ccaccgaaat gaccagcccc aaggccatgt agcaggaaag
      121 gaaaaggaag gtgcccgcca aaaagaccag gtggctgccc aaaaagggga tgccgaaata
      181 gaagcgggcc aaaaggtaga tgagcagttg ggctcccagg cccagcacca cgtagggcgc
      241 cagcttgccc aggatgatct ccagcggccg ggccggggtg gcgagcagca gttccatcga
      301 gccgttctcc cattcccgcg ccaccgtgag ggcggtcagc aggatggaga tgatggccag
      361 caccaccgcc gaaaggccgg gcacggtgaa ccagcggctg ttgagctcgg ggttgaagag
      421 gtagcgggtc ttcagggtaa agtcgggcgg tggggcgtcc ccgcccaggc gggcggaggc
      481 ggcggcctgg atgccggtca ggtaggcccc caggatgccc gcggtggaat tgtcgctgcc
      541 gtcgatgagc acctgggccg gggaaccgtc ctcggaggcc aggtaccgcc cgaaacgggg
      601 cgggatgacc agcacggcct tggcccggtc ggcgtcgagc ggcgccgtga cgtcggcggc
      661 caggggagcc tgccgcagct tgaaatagcc ggagctttca aaggcctccg ccagctggcg
      721 ggaagcccgg gaacggtcct ggtcatagac ggtcaggtgg atgtcgtgca cgttgaagtc
      781 catggccacg ccgaagaaaa gcaccatcaa gagcgggagc cccagggcca gccccagggt
      841 gaaggggtcc cggacgatgt gccgaacttc cttcagggcg atggaagcgg cccggtgtgg
      901 gttgagggac gcgctcattg tttcccctcc accaggcgga tgaagacgtc ctccaaagaa
      961 ggtgggatgc gccgcacctt gaccttgccc tggagccgcc taagcgccgc cgaccaggcc
     1021 cggggggcgc cggccacggc gtggtaccgc aggccgtggg gcgagaggga cctcacggcc
     1081 ccatcctttt ccaggcctcg ggcccagccg cgtggggcgt ggggctgggg cgagagctcc
     1141 cagaggggtt cggggaaggc gcttttcttg agggccgcgg ggctgttgag ggcgatgatg
     1201 cgcccgtcgc gcatgagggc gatgcggccg cactgctcgg cctcgtccat gtaatggg
//
LOCUS       DASTJJ010000779         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_963871, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000779 DASTJJ010000000
VERSION     DASTJJ010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963871"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFR02_09400"
     CDS             <1..397
                     /locus_tag="VFR02_09400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9870694.1"
                     /translation="RMVAQHLQQYLGDRAFRYGGEEFCAVFEDMEGAEALALAEAMRE
                     KLESRKFVIRRRLKTRGRKAPKAGDPAGQGVHITLSIGLACQDKKHPTWAEVLTRADQ
                     CLYQAKEKGRTRSVLAGAPAETGPSQGAS"
     gene            449..1684
                     /locus_tag="VFR02_09405"
     CDS             449..1684
                     /locus_tag="VFR02_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870695.1"
                     /translation="MRKFWTVAFSLAWLGAQAAWADLADRGGHYGNKHDREVGDGGNG
                     VHDNGARDGGGWNAGARDKGARPEAEGGPSRPIRYREENSRVERQAVHPRAATPAWRS
                     RATHVNQARPEAYEPRHFYRVESAKVPVPNRFKHLGVGSLPRPLTDRSHYVTSNRAHS
                     NIPLPALGPQGGRLSAHLVARTQIGSPLVRQHMAAFSSNVELDRINHYNRVETIPNHY
                     YWHTYGGYSYCHYYDPWGYHWYGWYVGDSCFWARYYWGNWWWYDPVWARWCYWHDGGW
                     WWNDPSNTTVVYVYENGAYQPADVAPDTTAAPPTAPSGNTSPQSAAPIPEKESFPSPD
                     GSRVVKIFGDSRDAVLSDQATPPKLESVFLGSNVKSVRFAATTDGGTQILVTYGDGTV
                     KNFDEDGHSLDDAPPNGDN"
     gene            complement(1952..>2137)
                     /locus_tag="VFR02_09410"
     CDS             complement(1952..>2137)
                     /locus_tag="VFR02_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9870696.1"
                     /translation="GSPAAASGLQPGDVVLKVGDRSVASSRQLEEALKKADLKDGVRL
                     FVWHNGVTVYAFLQDGD"
BASE COUNT          408 a          690 c          687 g          352 t
ORIGIN      
        1 gcgcatggtg gcccagcacc tgcagcagta tttgggcgac cgggccttcc ggtacggcgg
       61 cgaggagttc tgcgccgtgt tcgaggatat ggaaggcgcg gaggccctgg cgctggccga
      121 ggccatgcgg gaaaaattgg agtcgcgcaa gttcgtcatc cgccgccggc tcaagacccg
      181 ggggcggaag gcccctaagg cgggagatcc ggccggacag ggcgtgcaca tcaccctgag
      241 catcgggctg gcctgccagg acaagaaaca cccgacctgg gccgaggtct tgacccgggc
      301 cgatcaatgc ctctaccagg ccaaggaaaa gggccggact cgttcggttt tggccggcgc
      361 ccccgccgaa accggccctt cccaaggggc gtcttaacta gagaaggtcc aagagggccg
      421 cggtccaccc gcgggaaaag aggacgacat gaggaaattc tggacggtgg ctttcagttt
      481 ggcgtggctg ggcgcccagg cggcttgggc ggacctggcg gaccggggcg gccattacgg
      541 gaacaagcat gaccgggaag tcggcgacgg cggcaacggc gtccacgaca acggcgcccg
      601 ggacggcggc ggttggaacg cgggggcccg ggacaagggc gcccgtccgg aagccgaggg
      661 cggcccttcc cggcccatcc ggtaccggga agaaaactcc cgggtggaac gtcaggccgt
      721 ccatccgcgt gccgccacgc cggcttggcg cagccgggcc acccacgtga accaggcccg
      781 gcccgaggcc tatgagccca ggcactttta tcgggtcgag tccgcgaagg tcccggtccc
      841 caaccgcttc aagcatctgg gcgtgggcag cctgccccgg cccctgacgg accgcagcca
      901 ttacgtcacc tccaaccggg cccattccaa catccccctg cccgccttgg ggccgcaagg
      961 ggggcgcctc tcggcgcacc tggtggcccg gacccagatc gggtccccct tggtccgcca
     1021 gcatatggcc gccttctcct ccaacgtgga actcgaccgc atcaaccact acaaccgggt
     1081 ggagaccatc cccaaccatt actattggca cacctatggg ggttattctt actgtcatta
     1141 ctacgacccc tggggctacc actggtacgg ctggtatgtg ggcgacagct gcttttgggc
     1201 gcgctactac tggggcaact ggtggtggta cgacccggtc tgggcccgct ggtgctactg
     1261 gcatgacggg ggctggtggt ggaacgatcc ctccaacacc actgtggtgt acgtatacga
     1321 gaacggcgcc tatcaacccg ccgacgtagc cccggacacc acggccgcgc cgccgacggc
     1381 gccttccggg aacacctccc cgcaaagcgc cgctcccatc cccgaaaagg agtccttccc
     1441 cagcccggac ggcagccggg tggtgaagat cttcggggac tcgcgggacg ccgtcctgag
     1501 cgaccaggcc acgccgccca agctggaatc ggtcttcctg ggatcgaacg tcaagagcgt
     1561 gcgtttcgcg gccaccaccg acgggggcac ccagatcctg gtgacctatg gggacggcac
     1621 ggtgaagaac ttcgacgagg acggccattc gctggatgac gccccgccga atggggacaa
     1681 ctgagggttt tgaagggtcg ctgaacggcc ccggtccctg aagggatgaa cgcgggaata
     1741 aaaaagcccc ggccaaaagg ccggggcttt tttttcggaa ctatggcttt tgcaaagcaa
     1801 gccaaagaga acttgaaaca atgataaaaa ctgatgaaat ctgatctttg aaaataaaaa
     1861 tcaggcttta tcagctttta tcagtgttcc tattgtcctt gctttgcgaa atgcatgatt
     1921 ccgttttttt tttaactccg ccgcgccggg atcagtcccc gtcctgcagg aaggcgtaga
     1981 ccgtcacgcc gttgtgccat acgaagaggc gcacgccgtc cttcaggtcg gccttcttca
     2041 aggcctcttc cagttggcgg gaggaggcca cggaacggtc ccccactttc agcaccacat
     2101 cgccgggttg cagcccggag gccgccgccg ggctgcc
//
LOCUS       DASTJJ010000780         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_838618, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000780 DASTJJ010000000
VERSION     DASTJJ010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838618"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFR02_09415"
     CDS             <1..406
                     /locus_tag="VFR02_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9870697.1"
                     /translation="VMLSHVIYKNVDPYFPASLSSEMIQTLLRRQLGYQGLVISDDLR
                     MDAIRDYYPLDVSVVQAVNAGVDVLLVTDNLEGRVMDSLVAAVRSGRIPMAVIDEAYG
                     RILATKKKYGIIPRPTPARAQAPGPLRLAQNP"
     gene            complement(414..992)
                     /locus_tag="VFR02_09420"
     CDS             complement(414..992)
                     /locus_tag="VFR02_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870698.1"
                     /translation="MMKAVWLLLAWAAPAGLWAGPGWVTVSFGGTAAEVCAPLPLPPH
                     GGLGVRGAFEYGPEEWTSLGLDFGLAEYIAPSGQQDMKSAWMDLTAAFFPWGPLGSDR
                     PYIRLGFGLSPYIGGLFEDYWPDYASRRLGRPEKAGNPYWTAQAALGYRFALDRRWAL
                     DLGMRYDRFWPPVDLPLQTLGVETGLVWCLTP"
     gene            complement(1023..>1273)
                     /locus_tag="VFR02_09425"
     CDS             complement(1023..>1273)
                     /locus_tag="VFR02_09425"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET9870699.1"
                     /translation="TGSVGILLVISGNEKVTRNRSAAVALFGLFLNLLLVPKWGMVGA
                     AVSTALCVASSNLISAYFVWSKLGIWTLPFPRWVGKRP"
BASE COUNT          251 a          437 c          387 g          198 t
ORIGIN      
        1 ggtcatgctg tcccacgtca tttataagaa cgtggaccct tatttccccg cgtcgctttc
       61 cagcgaaatg atccagaccc tgctgcgccg ccaattgggt taccagggcc tggtcatctc
      121 ggacgacctg cgcatggacg ccatccggga ctactatccc ctggacgtgt cggtggtgca
      181 ggcggtgaac gcgggggtgg acgtgctgct ggtgacggac aacctggaag gacgcgtgat
      241 ggacagcctg gtggcggcgg tgcggtcggg ccgcatcccc atggccgtca tcgacgaagc
      301 ctatgggcgc atcctggcca ccaagaagaa gtacggcatc atcccccgtc ccacgcccgc
      361 ccgcgcgcag gcgcccgggc ccctgcgcct ggcccagaac ccctgatccc gggctaaggg
      421 gtcaagcacc acaccaggcc cgtttccacc ccgagggtct gcaggggcag gtccaccggg
      481 ggccagaaac ggtcgtacct catccccagg tccagcgccc aacgacggtc cagggcgaac
      541 cggtatccca aggcggcttg cgccgtccaa taggggttgc cggctttttc cggacggccc
      601 aaccgtctgc tggcgtaatc cggccaataa tcctcgaaca ggcccccgat atagggggaa
      661 aggccgaatc ccaaccggat ataaggccgg tccgatccga gcgggcccca tggaaaaaag
      721 gcggcggtca ggtccatcca ggcgcttttc atgtcctgtt ggccggaggg ggcgatgtat
      781 tccgccaacc cgaagtccaa ccccaaactg gtccattctt ccgggccata ctcgaacgcc
      841 ccccggacgc cgagcccgcc atgggggggc aggggcagcg gcgcgcaaac ctcggctgcg
      901 gtcccgccga aggagaccgt gacccaccct gggccggccc aaagccccgc cggggccgcc
      961 caagccagaa ggagccaaac cgctttcatc atgggggcgc cttttcatat agaagcgtgg
     1021 acctaaggcc ttttccccac ccaacggggg aacggtagtg tccagatccc gagtttcgac
     1081 cacacgaaat aggcggatat caggttgctc gacgccacgc acaaggccgt cgacaccgcc
     1141 gcccctacca tcccccattt ggggaccaat aaaaggttga ggaacaatcc gaacaaggcc
     1201 acggccgccg accggttccg cgtcaccttc tcgttgccgc tgatgaccaa gaggatcccg
     1261 acggatccag tgg
//
LOCUS       DASTJJ010000781         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_473714, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000781 DASTJJ010000000
VERSION     DASTJJ010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473714"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..640
                     /locus_tag="VFR02_09430"
     CDS             44..640
                     /locus_tag="VFR02_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase 2"
                     /protein_id="HET9870700.1"
                     /translation="MSPVGINLGKSKIAPLEDAVEDYLEALEALAPYADYLAVNVSSP
                     NTPGLRKLQEAKPLRGLLRAFVRGSLGKPVLLKLAPDLEGKPLREAADIALSAGCAGL
                     IATNTTITRLSLPPGEYPVGGLSGGPLRDKADAVLRDLSKFTRGRVPLVGVGGILSAG
                     DVARKFEAGASLVQVYTGYVYGGPGFPARLCRELLKKA"
     gene            complement(753..>1631)
                     /locus_tag="VFR02_09435"
     CDS             complement(753..>1631)
                     /locus_tag="VFR02_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9870701.1"
                     /translation="YLLALLGLYRAVIFIHELAHLKKDSFPVFRAVWNLVCGFPLMVP
                     SFTYMGVHIDHHKQKVYGTQEDGEYLPFVLVGRFRIILFLVMMGVAPAFVAFRFLVLT
                     PLSYLVPPLRRLLWQRASSLVIDESYVRPEPADRDGRWWRLQEFLTFLYALAGAVLLW
                     KGVLPWKAFFVWYAVAGGILFVNGVRTLVAHCYRNPADHVLEFSEQFLDSVTVPGLDF
                     VTPLWAPVGLRFHAVHHLFPGMPYHHLAEAHRRFVRELPPNSSYHLTLRKDLWDALGR
                     LWKETKEYQRSKKGKS"
BASE COUNT          326 a          491 c          545 g          269 t
ORIGIN      
        1 ggccgccaaa tagaaggcct tgaaggaaaa gggccgctgg cccatgagcc cggtggggat
       61 caacctgggc aagagcaaga tcgcgcccct ggaggacgcg gtggaggact atctggaagc
      121 gttggaagcc ttggcgccct acgcggatta tttggcggtc aacgtcagtt cccccaacac
      181 gccgggcctg cgcaaactgc aggaagccaa gcccttgcgg gggttgttgc gggccttcgt
      241 gcgggggtcc ctgggcaagc ccgtgctctt gaagttggcg cccgacctgg aaggcaaacc
      301 cctgcgggag gccgccgaca tcgccttgtc cgccggctgc gcgggattga tcgccaccaa
      361 caccaccatc acgcggctga gcctgccgcc gggcgagtat ccggtgggcg gcctgtccgg
      421 gggccccctg agggacaagg ccgacgccgt gctgcgcgat ctttccaaat tcacccgggg
      481 aagggtgccg ttggtgggcg tggggggcat cttgtcggcg ggggatgtgg cgcgcaaatt
      541 cgaggcgggc gcctcgttgg tgcaggtcta cacgggctac gtctacgggg gaccgggctt
      601 ccccgcccgg ctctgccggg aactgctcaa aaaagcctga accagccgga ccaccaatgc
      661 acgtcgatgg gcggggataa actaaaggtc attcggatct ttgtccaaaa gaattcatcc
      721 gtgtatatcc gtgttcatct gtggttaaaa ttttaacttt tccctttttt gctcctctgg
      781 tattcctttg tttccttcca aagccgtccc aaagcgtccc acaggtcctt gcgcagggtg
      841 aggtggtagg agctgttggg gggcagttcc cgcacgaacc ggcggtgggc ttccgccagg
      901 tggtggtagg gcatgccggg gaagagatgg tggacggcgt ggaaccgcag tcccaccggg
      961 gcccagagcg gcgtgacgaa atccaacccc ggcacggtca ccgaatccag gaactgctcg
     1021 gaaaattcca gcacatggtc cgcgggattg cggtagcagt gggccaccag ggtgcggacc
     1081 ccgttgacga acaagatgcc tcccgccacg gcgtaccaca cgaagaaggc cttccagggg
     1141 aggacgccct tccacagtag gacggccccc gccaaggcat agaggaaggt gaggaattcc
     1201 tgcaggcgcc accagcgccc gtcccggtcc gcgggctcgg gccgtacgta gctttcatcg
     1261 atgaccaggg acgaggcccg ctgccagagc agccgccgca gggggggcac caggtaggaa
     1321 aggggcgtca ggaccaggaa ccggaaggcc acgaaggccg gcgccacgcc catcatgacc
     1381 aggaacagga tgatcctgaa ccgccccacc agcacgaaag gcaggtattc cccgtcctcc
     1441 tgggtgccgt agaccttttg cttgtggtgg tcgatgtgca cgcccatata ggtgaaggaa
     1501 ggcaccatca ggggaaaacc gcacaccagg ttccagaccg cacggaacac cgggaaactg
     1561 tccttcttca agtgggccag ctcatggatg aagatgaccg cccggtagag gcccagcagg
     1621 gccaacaggt a
//
LOCUS       DASTJJ010000782         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_473729, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000782 DASTJJ010000000
VERSION     DASTJJ010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473729"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFR02_09440"
     CDS             <1..189
                     /locus_tag="VFR02_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870702.1"
                     /translation="LQGRKDSMVAPENGERVWRSLGSKRKHLVYLDRSDHVIPLDYDR
                     GLLFEKTLRFLKSGGRRA"
     gene            322..1239
                     /locus_tag="VFR02_09445"
     CDS             322..1239
                     /locus_tag="VFR02_09445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9870703.1"
                     /translation="MSHPIRSYFKKKITFMVIPHDGEKSAHLKMHLCLVHGVLLAAAG
                     LVGWALYLSGQDVDYRLARLNDQRIRASMQEVADELQTNRQLLSGMAGVDSQFRKLLK
                     LGDRKTVLEYNGMGGPTEQDSSRFSKLLEEKNEQLLTKIQAGLQQSNREASRQEASFR
                     QISLYLDKQRSILAATPDIWPVKGWVTSGFGKRMNPLTDEPGVHMGVDIANEVNTPIH
                     ATADGLVTYAGWESGYGRVVVIEHGYGFSTRYGHCNRLEVKVGDEVKRGQVIGYMGST
                     GMSTGSHCHYEVRIHGVPVNPIPYLPKID"
     gene            1291..1689
                     /locus_tag="VFR02_09450"
     CDS             1291..1689
                     /locus_tag="VFR02_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9870704.1"
                     /translation="MGLFTFGTPAKGIGRVETIIGADASLRGSYNSKHSIRVDGEIYG
                     NVVSEDGIILGPKGLIRGNITAKTVLVGGRVKGNITAYQRLEFQSTARVEGDLSTPTL
                     FVEEGAQFEGNSQMEEKAKVLDMPKARDNG"
     gene            1963..>2326
                     /locus_tag="VFR02_09455"
     CDS             1963..>2326
                     /locus_tag="VFR02_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9870705.1"
                     /translation="MKAKKKTKSPKAVYLDCAATSLQKPPQVLRALVDYASSVGVSHG
                     RGAYGRGLAANAMVYGTRAALGKLLGVTRTDRILFTKNVTEALNTALKGWLREGDHVV
                     LSGLEHNAVIRPLNKLKAQ"
BASE COUNT          466 a          747 c          726 g          387 t
ORIGIN      
        1 ctgcagggcc ggaaggattc catggtggcc cccgagaacg gagagcgggt ttggcgttcc
       61 ttggggtcca agcgcaagca cctggtctac ctggaccgct cggaccacgt gatcccgctg
      121 gactatgacc ggggcctgct cttcgaaaag accctgcgtt tcctcaagag cgggggccgg
      181 cgggcctgac cccccccagg gcttggggaa cctttacccg cagcgggggg cttccccgtt
      241 gacaggccgg aaccccgcaa ttattttggg ggttgggccc aagcgcccga acccccgaga
      301 ccaggagccc ttcccacgcc catgtcccat cccatccggt cttacttcaa gaaaaagatc
      361 acgttcatgg tcatccccca cgatggggag aagtccgccc acctcaagat gcacctgtgc
      421 ctggtgcacg gcgtcctgct ggcggcggcg gggctggtgg gctgggccct ctacctgtcg
      481 ggccaggatg tggactaccg cttggcccgc ctcaacgacc agcgcatccg tgccagcatg
      541 caggaggtcg ccgacgaact gcagaccaac cgccagctcc tctcgggcat ggccggggtg
      601 gattcccagt tccgcaagtt gctcaagctg ggcgaccgca agaccgtgct ggaatacaac
      661 ggcatgggcg gccccaccga acaggattcc tcgcggttct ccaagctgct ggaggagaag
      721 aacgagcagc tgctcaccaa gatccaggcg ggcctgcagc agagcaaccg ggaagccagc
      781 cggcaggagg ccagtttccg ccagatcagc ctgtacctgg acaagcagcg ttccatcctg
      841 gcggccacgc ccgacatctg gcccgtcaag ggctgggtca cctcggggtt cggcaagcgc
      901 atgaaccccc tgaccgacga gcccggggtg cacatggggg tggacatcgc caatgaggtg
      961 aacacgccca tccacgccac cgccgacggg ttggtcacct acgcgggatg ggaatcgggt
     1021 tatggccggg tggtggtcat cgagcacggc tacggcttca gcacccgcta cggccattgc
     1081 aaccggctgg aggtcaaggt gggcgacgag gtcaaacggg gacaggtcat cggctacatg
     1141 ggctccaccg gcatgagcac gggcagccat tgccattacg aggtacgcat ccacggcgtg
     1201 cccgtgaacc ccatccccta cctgcccaag atcgactgac ccagtccccc gccgcggacg
     1261 ggggaccaca gacccaccag gaggcgcagc atgggacttt tcacgttcgg gaccccggcc
     1321 aaaggcatcg gtcgggtgga gaccatcatc ggcgccgacg cgtcgctgcg gggcagctac
     1381 aattccaagc acagcatccg ggtggacggc gagatctacg gcaacgtggt cagcgaggac
     1441 ggcatcatcc tgggccccaa gggcctcatc cgcggcaaca tcacggccaa gaccgtcctg
     1501 gtgggcgggc gggtcaaggg caacatcacg gcctaccagc ggctggagtt ccaatccacc
     1561 gcccgggtgg aaggcgacct ttccacgccc acgctgttcg tcgaagaggg cgcccaattc
     1621 gagggcaaca gccagatgga agagaaggca aaggtgttgg acatgccgaa agcgcgggac
     1681 aacggctgat ccttccggct tgaaaaaggc ggcctgcaaa ggccgccttt ttttattggg
     1741 atcgttcact tcccaaagca aaggccattg gaacattgat agaggctgat aaatattgac
     1801 ttttattttc agacatcaga tttcatcaat ttttatcatt gtttcaaaag cctgctttat
     1861 tcgctttgta aaagacatag ttccgttttt tattgggccc cttcgtttga ttcgcgccgg
     1921 gcgcggccct atgatgacgg ggccgttctt gaagagggga ccatgaaagc caaaaagaag
     1981 accaaaagcc ccaaggccgt ctacctggac tgcgcggcca ccagcctgca gaagccgccc
     2041 caggtcctgc gggcgctggt ggattacgcc tcctccgtcg gcgtgtccca cggccggggc
     2101 gcttacgggc gggggctcgc cgccaacgcc atggtctacg gcacccgcgc ggcgctgggg
     2161 aagctcttgg gcgtcacccg cacggaccgc atcctcttca ccaagaacgt gaccgaggcc
     2221 ctgaacacgg ccctgaaggg ctggcttcgg gaaggcgacc acgtggtcct gtcggggctg
     2281 gagcacaacg ccgtcatccg ccccctgaac aagctgaagg cccaac
//
LOCUS       DASTJJ010000783         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_716526, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000783 DASTJJ010000000
VERSION     DASTJJ010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716526"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1268)
                     /locus_tag="VFR02_09460"
     CDS             complement(<1..>1268)
                     /locus_tag="VFR02_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870706.1"
                     /translation="AVVLFYFAGKEAGGIWVGLVAEAAAAFSKPLLIENLIGMTAVTV
                     PLAVALLLWTTFRLFNRTSWGRFIQWGLALGFAPYNYTPIRTWLPFIITVVLAWLFFH
                     KLKGRPKSWVEILLGCGTLTVWILGFLKVNNFLPESDLNLGAAAKGAVVMLLAALAVV
                     QWRGHQRESDRNWVSAFFLSVLLATALMYPLASDPEISVHTSGLSIFHNQETRTFHYE
                     NSVLDQMLKKTGNTLETMFVGGQDRGDLNLPWDAFFEYHFLPLLLLGLAAFLSRPGWL
                     SLFLVLGGFVGISTHILSIDPHSAKMVAAVAPLAILCGLGIRQLAASLSARSRKVSLG
                     ISFAVLAYLGWAVWGTYDRVWGHYFKIGGVDKIVSQAVGDLADRYRLYMGVYRDFASP
                     ESQSLLDEGHNVYLLQPYNPIFLRPGEAPR"
BASE COUNT          306 a          400 c          369 g          193 t
ORIGIN      
        1 ccggggcgcc tcgcccggcc gcagaaagat gggattgtag ggctggagca agtagacgtt
       61 gtgcccctca tccagcaagg attgggattc gggcgaggca aaatccctgt aaacgcccat
      121 gtaaagccgg tatcgatcgg ccaaatcccc cacggcctgg gaaacgatct tatccacgcc
      181 cccgatcttg aaataatgcc cccagacccg gtcataggtt ccccaaaccg cccagcccag
      241 gtaggccagg acggcgaacg agatcccgag tgaaaccttc cgggatcgcg ccgacaagga
      301 ggcggccaac tgccggatcc ccaagccgca taggatcgcc aaaggcgcca ccgccgccac
      361 cattttggcg ctgtggggat cgatggaaag gatgtgggtg gagatcccca cgaaacctcc
      421 caaaaccaag aacagggaaa gccatccggg acggctcaaa aaagcggcca accccagcag
      481 gagcaggggc aggaagtggt actcaaagaa agcgtcccaa ggaaggttca agtcgccgcg
      541 gtcttgcccg cccacgaaca tggtctccag ggtgttgccc gtctttttca gcatctggtc
      601 gagcacggag ttctcgtaat ggaaggtccg ggtctcctgg ttgtggaaga tcgaaaggcc
      661 cgacgtatgg accgagatct ccggatcgga ggccaaggga tacatcaagg ccgtggccaa
      721 aaggacgctg aggaagaaag ccgacaccca gttccggtcc gactccctct ggtgccccct
      781 ccattggacg accgcgaggg ccgccaggag cataacgacc gcccccttgg ccgccgcccc
      841 aaggttcagg tccgactcgg gcaaaaaatt attgaccttc aagaacccga ggatccaaac
      901 cgttaaagtc ccgcatccca acaggatctc cacccaggat ttcgggcggc ccttcaattt
      961 gtggaaaaac aaccacgcca agaccacggt gatgatgaag ggcaaccagg tcctgatcgg
     1021 ggtgtagttg tacggcgcga atcccaaggc caggccccat tggatgaatc gaccccagct
     1081 ggtccggttg aacaggcgga aggtcgtcca caggagcagg gccaccgcca gtgggaccgt
     1141 caccgccgtc attccgatca agttctcgat caacagcggc ttgctgaagg cggccgcggc
     1201 ttccgccacc agaccgaccc agatcccgcc cgcttccttg cccgcgaagt aaaaaaggac
     1261 caccgcgg
//
LOCUS       DASTJJ010000784         1206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_594536, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000784 DASTJJ010000000
VERSION     DASTJJ010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594536"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /gene="rpmH"
                     /locus_tag="VFR02_09465"
     CDS             complement(<1..96)
                     /gene="rpmH"
                     /locus_tag="VFR02_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9870707.1"
                     /translation="MKRTYQPNRRKRQKVHGFRSRMKTVGGRRVLS"
     gene            228..764
                     /gene="cobU"
                     /locus_tag="VFR02_09470"
     CDS             228..764
                     /gene="cobU"
                     /locus_tag="VFR02_09470"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET9870708.1"
                     /translation="MKPHLTLILGGARSGKSRFALGLNPSPGPGSVFLATGTPGDDEM
                     RERIQKHRQERPAGWETRESPYDLGGALAASASAPGSVVLVDCLTLWLSNLLCGVGGD
                     ALGKDAVEEKIAALQKSLGALQGRVLFVSNEVGWGLVPDSPLGRDFRDLQGRLNQSIA
                     EVSDDIFLLVAGIAQKIK"
     gene            881..>1206
                     /locus_tag="VFR02_09475"
     CDS             881..>1206
                     /locus_tag="VFR02_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870709.1"
                     /translation="MKTKNSPSAPKWREGLLVLNRLWQRGLFPADLEKHYFLHWFVIY
                     GGNIIKTAAALKIHRNTIQGHFLKLGYSRRSVKLRHAWQKLGSAKGPFESRFGAFYRK
                     FAARPK"
BASE COUNT          217 a          378 c          366 g          245 t
ORIGIN      
        1 actcaagacc cggcgcccac cgacggtctt catgcggctg cggaaaccgt ggactttttg
       61 gcgcttgcgg cggttgggtt ggtaggtgcg tttcatggtc tctatcctta tttatatggc
      121 tctggccctg aggggcaaaa gagggacaat tgtatcccag tcccgccgaa aaggtcaatc
      181 acggcctggg ccttggccca tcccgcgaag catgctacaa aggccccatg aaaccccact
      241 tgaccctcat tttagggggt gcccgttcgg gtaagtcccg gttcgccctg gggctgaacc
      301 cctcccccgg gcccggcagc gtctttttgg ccaccggcac cccgggggac gatgaaatgc
      361 gggagcgtat ccagaagcac cggcaagaac ggcccgccgg ttgggagacc cgggagtcgc
      421 cctacgacct agggggggcc ttggcggcct cggcctcggc cccggggtcg gtggtgctgg
      481 tggactgcct cacgctgtgg ctgtccaacc tgttgtgcgg ggtgggcggg gacgcgctgg
      541 ggaaggacgc ggtcgaggaa aagatcgccg cgttgcaaaa aagtttgggc gcgctccaag
      601 gccgggtgct tttcgtgtcc aacgaggtgg gctgggggct ggtgcccgac tcccccttgg
      661 gccgggactt ccgcgacctg cagggaaggc tcaaccaatc cattgctgaa gtttcggacg
      721 atattttcct gctggtggcc gggattgccc aaaaaataaa ataatttttc cggggatgtt
      781 gtccgcttcg tacgtttggc gcgttccatt ttttgtcccg ttgatttccc cgggcgcgag
      841 cctttacaaa acgccgcgcc ttcattacaa caacccctca atgaaaacca agaactcacc
      901 gtccgcgccc aaatggcgcg agggattgct ggtcctcaac cgcctgtggc agcggggcct
      961 ctttcccgcg gacctggaaa aacattactt cctccactgg ttcgtcatct acggcggcaa
     1021 catcatcaag acggccgccg ccctcaagat ccaccgcaac accatccagg gccacttcct
     1081 caaattgggc tactcgcgcc ggtcggtcaa gctgcgccac gcctggcaaa aattgggatc
     1141 cgcgaagggc cccttcgagt cccgcttcgg cgccttctac cgcaagttcg cggcgcggcc
     1201 caagtt
//
LOCUS       DASTJJ010000785         1271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1574716, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000785 DASTJJ010000000
VERSION     DASTJJ010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574716"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFR02_09480"
     CDS             <1..938
                     /locus_tag="VFR02_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9870710.1"
                     /translation="MKISLFFLVLCLALVVSLTSSGGFTWKHLFHALGWILIILFVAF
                     LGLRRMFGPMRHLMRGVMELSNGNLDFRFPPKGRGEFDFLAFNFNYMADRIQDMMRSK
                     DQLLLDVSHELRSPLTRLKLALEMAPGHKLKASMMEDIAEMESLLAALLETQQLKSDH
                     GKLRLEELDLSALLAETAAKYQGRAPGLALRPGAPAVARVDGARARTVFQNLLENALK
                     YSAHQSKPVEISIEPRDGAVWVEVRDHGSGVPPADREKIFEPFYRVDKSRAKETGGYG
                     LGLSLCREIMRAHGGSITLEDRPGPGCVFRLVFPR"
     gene            1144..1271
                     /locus_tag="VFR02_09485"
                     /pseudo
     CDS             1144..1271
                     /locus_tag="VFR02_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982494.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
BASE COUNT          216 a          431 c          397 g          227 t
ORIGIN      
        1 tcatgaagat ctcccttttc ttcctggtgc tttgtttggc cctggtggtc agcctgacct
       61 ccagcggggg cttcacctgg aaacacctct tccacgccct gggctggatc ctgatcatcc
      121 tcttcgtggc cttcctgggc ctgcggcgca tgttcgggcc catgcgccac ctcatgcggg
      181 gcgtgatgga actttccaac ggcaacctgg acttccgctt ccccccgaag ggccggggcg
      241 agttcgactt tttggccttc aacttcaact acatggccga ccgcatccag gacatgatgc
      301 gctccaagga ccaactgctc ctggacgtga gccacgaatt gcgcagcccc ctcacccggc
      361 tcaagctggc cctggagatg gcgcccgggc acaagctgaa ggcgtccatg atggaggaca
      421 tcgccgagat ggaatccctg ctggcggcgc tcttggagac ccaacaactc aagagcgacc
      481 acgggaaatt gaggctggag gaattggacc tgtccgccct cctggccgag accgccgcca
      541 aataccaagg ccgcgccccc ggcctggccc tgcgccccgg cgcgcccgcc gtcgcccggg
      601 tggacggcgc gcgggcccgc acggtcttcc agaacttgct ggagaacgcc ttgaaatatt
      661 ccgcccacca atccaaaccc gtggaaattt cgatcgagcc ccgggacggg gccgtgtggg
      721 tcgaggtgcg ggaccacgga tcgggcgtcc cccccgccga ccgggaaaag atcttcgagc
      781 ccttctaccg ggtggacaag tcccgcgcca aggagaccgg cgggtacggc ctggggcttt
      841 ccctctgccg ggagatcatg cgggcccacg gcggttccat cacgctggag gaccgccccg
      901 gccccggctg cgtcttccgg ctggttttcc cccgctgaag gggccgccgc gaaaagaccc
      961 ccgccccgtg gcttttcgcg ggtcccgggc gtctaatggg ggacagatca tgaacgggga
     1021 gggatcatgc gtatcctgac ggcggggttg gcggttttcc tgggactctt gggcgcgggg
     1081 actttgaagg cggccccggc gcccggggcg gacggcacgg tctacggctt ccaggccgaa
     1141 tccatcttcg gcaagacggt ccacctgtcc cactactagg gccgggtgct gctcatcgtc
     1201 aacgtggcca gcaaatgcgg cttcacgccc caatatgacg gcctggaaac cctgtataaa
     1261 aaatacaagt c
//
LOCUS       DASTJJ010000786         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_595123, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000786 DASTJJ010000000
VERSION     DASTJJ010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595123"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VFR02_09490"
     CDS             <1..871
                     /locus_tag="VFR02_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870711.1"
                     /translation="LNFAYYGKKKKDEKVQAMADRIKARLSPEDQDWVDQQLATEPPF
                     PPPFRYSGIGLRLGADVQFAALADFNDYAKTLKGMGVSAASPAVALGAEISPFVQVID
                     GLEAGFSLGGIFLGGFSASTNSASPQQNGNIDYDLWDGGLDLKATFLRADRGKIRFFL
                     EADPRAYLGSLSVVNSDTSVNWGFLPVNGNFSTTGFGGVLKLGMEWKPLPNSLVGFFA
                     GYQMADLTGFTGSGAANGSSTAVPGKLVTVRDPSGTRLEFVPDGSPDPAGASPTALDL
                     GGVVAGAQLTALF"
     gene            917..1531
                     /locus_tag="VFR02_09495"
     CDS             917..1531
                     /locus_tag="VFR02_09495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870712.1"
                     /translation="MAQGFSFYDRLYLSLFYALKLRRYDLPEQRVGWQSRRNQEARFE
                     ALTGVGDLLGRRVLDLGSGLGCLYGYLRERGWRGDYTGFDLLGPMTREARRRFPEARF
                     ETRDILADPPREQWDYALVSGVFNHRVKDNWAWTAAMVGALRGLAAKGVAFNLLSSEL
                     GEWDPELFYAHPRELERRAADWSGGNYRLVTGYLPGDVTVYLYP"
     gene            complement(1565..2215)
                     /locus_tag="VFR02_09500"
     CDS             complement(1565..2215)
                     /locus_tag="VFR02_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870713.1"
                     /translation="MRNIFLALALLGLAWPRTALAGFSLELMGGDALNFPSPLSIHQA
                     GYPDLDFTARYDTRPYNAYAPYYAWRASLWDGSQAWELEQVHHKLFLTDPPPQVQAFA
                     IHYGYNYFFVNHAWRYPDLILRLGTGPIITNPSNTVRGQVLVTHGTGWFDQGYDFSGW
                     GAQVSAARDLGLFDTTYLVAELALIGGWAWDVPVANGSADVPTLGLHFHLGLGLDL"
     gene            2296..2640
                     /locus_tag="VFR02_09505"
     CDS             2296..2640
                     /locus_tag="VFR02_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET9870714.1"
                     /translation="MANDCLFCKFVRKEIQPNLAYEDDQVMVFHDIHPQAPVHALVIP
                     KKHIDSVTAIPSGDPIVSLLTERAVKVAAQLGIAQEGFRLVFNNGHNGGQTVSHLHLH
                     LLGGRPMGWPPG"
     gene            2782..2982
                     /gene="rpsU"
                     /locus_tag="VFR02_09510"
     CDS             2782..2982
                     /gene="rpsU"
                     /locus_tag="VFR02_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET9870715.1"
                     /translation="MVEVRLEENEPIENALKRFKKVVQNSGLISEMKKREFYEKPSER
                     RKKREAAARKKARRRQMKFSRD"
BASE COUNT          560 a         1073 c         1080 g          518 t
ORIGIN      
        1 cctcaacttc gcctattacg ggaagaaaaa aaaggatgag aaggtccagg ccatggcgga
       61 ccgcatcaag gcccgcctga gccccgagga ccaggattgg gtggaccagc agttggccac
      121 cgagccgccc ttcccgccgc ccttccgcta ttcggggatc ggcctgcgcc tgggcgcgga
      181 cgtgcagttc gccgccctgg cggatttcaa cgactacgcc aagaccctga agggcatggg
      241 ggtgtcggcc gcctcgcccg cggtggccct gggggcggaa ataagcccct tcgtgcaggt
      301 gatcgacggg ttggaggccg ggttctccct cggggggatc ttcctcgggg gattttcggc
      361 ctccaccaac agcgccagcc cccagcagaa cggcaacatc gactacgacc tttgggacgg
      421 cgggctggac ctgaaggcca ccttcctgcg ggccgaccgg ggcaagatcc ggttcttcct
      481 ggaggccgac ccccgggcct acctggggtc cctgtcggtg gtcaattcgg acacctcggt
      541 caattggggt ttcctgcccg tcaacggcaa cttctccacc acgggctttg ggggcgtcct
      601 caagctgggg atggaatgga agcccctgcc gaactcgctg gtgggttttt tcgccggcta
      661 ccagatggcc gacctgacgg gcttcacggg ctcgggggcc gccaacggct cctccaccgc
      721 cgtgccgggc aagctggtca cggtgcggga cccctcgggc acccggctcg aattcgtccc
      781 cgacggctcc ccggaccccg cgggggcctc ccccacggcg ctcgacctgg gcggggtggt
      841 ggcgggcgcc caactgaccg cgcttttttg acccccgcgg cttggcgccc gggcccgctt
      901 ggggtctaat gcccccatgg cccagggctt cagcttttac gaccggctct acctttccct
      961 cttttacgcg ctcaaactgc gccgctacga cctgcccgaa caacgggtgg gctggcagtc
     1021 gcgccgcaac caggaagccc gcttcgaggc gctcacgggc gtgggcgacc tgttgggccg
     1081 ccgcgtcctg gacctgggtt cggggttggg ctgcctctac ggctacctgc gggaaagggg
     1141 ttggcggggc gactacacgg ggttcgacct cctggggccc atgacgcgcg aggcccgccg
     1201 gcgtttcccc gaggcgcgct tcgagacccg ggacatcctg gccgacccgc cgcgggaaca
     1261 atgggactac gcgctggtga gcggggtctt caaccaccgg gtgaaggaca actgggcctg
     1321 gacggcggcg atggtggggg cgctgcgggg cctggcggcg aagggggtgg ccttcaacct
     1381 gctctcctcc gagctggggg aatgggaccc ggagcttttc tacgcccacc cgcgggaact
     1441 ggaacgccgg gcggccgatt ggtcgggagg gaactaccgg ctggtcacgg gctacctgcc
     1501 gggcgacgtg acggtctact tgtatcccta gggcgtccgc ccggacggaa acgccggtcc
     1561 tttttcagag gtcgaggccc aggcccagat ggaagtggag ccctagcgtc ggcacgtccg
     1621 cgctgccgtt ggcgaccggc acgtcccagg cccagccgcc gatgagggcc agctccgcca
     1681 ccagataggt ggtgtcgaag aggcccaggt cgcgcgccgc cgacacctgg gcgccccagc
     1741 ccgaaaaatc atagccctgg tcgaaccagc ccgtgccatg ggtcaccagc acctggcccc
     1801 gcaccgtgtt ggaggggttg gtgatgatgg gccccgtgcc caggcgcagg atgaggtccg
     1861 gataacgcca ggcgtggttc acgaagaaat aattgtagcc gtaatggatg gcgaaggcct
     1921 gcacctgggg cggcgggtcg gtgaggaaga gcttgtggtg cacctgctcc aattcccagg
     1981 cctgggagcc gtcccagagc gaggcgcgcc aggcgtaata gggcgcgtag gcgttgtagg
     2041 gccgggtgtc gtagcgggcg gtaaagtcga ggtccgggta gcccgcctgg tggatcgaaa
     2101 ggggcgaggg gaagttgagg gcgtcgccgc ccatcagttc cagggaaaag ccggccaggg
     2161 cggtccgggg ccaggccagg cccagcaggg ccaacgccaa aaatatattc cgcaagcgcc
     2221 cccctttcct tgtccggtac ggatgttata gtaggggcaa ggccggtcag cctcaacctg
     2281 ttcccgggag cgcccatggc gaacgattgc ctcttctgca agtttgtccg caaggagatc
     2341 cagcccaacc tggcctatga ggacgaccaa gtgatggtct tccacgacat ccatccccag
     2401 gcgccggtgc acgcgctggt catccccaag aaacacatcg attcggtcac ggccatcccc
     2461 tcgggggacc ccatcgtgtc cctgctcacc gagcgggccg tcaaggtggc cgcccagttg
     2521 ggcatcgccc aggaaggttt ccggctggtc ttcaacaacg gccacaacgg cggccagacc
     2581 gtttcccacc tgcacctgca tcttttgggc ggccgcccca tgggctggcc cccgggctga
     2641 ccccccaaaa accctgcaaa aggcccctgg ggggcgtccc ggccttgctt gactcccccc
     2701 ggcgaaaggt ttaaactccc cttcccccgg aggcctggcc tcccgggacc gcatcccacc
     2761 cacagagggg agagtgacca cgtggtcgaa gtccgattgg aagagaacga gcccatcgag
     2821 aacgccctca agcgtttcaa gaaggtcgtg cagaactccg ggctgatctc ggagatgaag
     2881 aaacgggaat tctacgagaa acccagcgag cgccgtaaaa agcgcgaagc cgccgcccgt
     2941 aagaaggcgc gccgccgcca gatgaagttc agccgcgact gaccgccggg cctagcggag
     3001 cccgttcccg ccacgggaac gggctctttt tgtttcccca aaccgttttg gaggttccca
     3061 tgagcgcatc ccccctgttc gacaagatca acgccgacct gatcacggcc atgaaggcca
     3121 aggacgaggc cgccctgtcg gcgctgcgca tgctcaaaag cgccatcaag tacaaggaag
     3181 tggacctgaa gcgcgagccc accgacgagg acgtcattga ggtgctctcc a
//
LOCUS       DASTJJ010000787         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_717563, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000787 DASTJJ010000000
VERSION     DASTJJ010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717563"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFR02_09515"
     CDS             <1..725
                     /locus_tag="VFR02_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9870716.1"
                     /translation="KVAKAREAGTGEDHPMYYIVVLVDELADLMMVAKDQVEESIARL
                     AQMARAVGIHLVLATQRPSVEVITGIIKANFPSRIAFQVFSKVDSRTILDMNGAEALL
                     GRGDMLYLPSGAPKPLRLQGAYVTLEEIERLVDFWKSQGRPLYTADLTTQKSRELVSE
                     QSEDELYDEAVQIILRAKQASTSHLQRRLKVGYSRAARLLDDMEARGIVGPADGNKPR
                     KILVPVPQDQTPDMDEETEGEA"
     gene            715..1386
                     /locus_tag="VFR02_09520"
     CDS             715..1386
                     /locus_tag="VFR02_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HET9870717.1"
                     /translation="MKLKTLKRLAALAACGWAAPLLGADAPAPLSLDQILDKVEAAQA
                     AAQDVQMDLKTRMRDTMSGQTQEVQGVAMLKAPDLVYVHYQKPTEQFLYIDGALAQMY
                     QPAQKMVYRQQAAPGSPDAPVYLGVGRELKHYADLSRVSIDHQSGDEVTLLFLPKSTD
                     AGFDRMRVTIRKKDWWPVRLEVETPALETRCEFSNFKFDQGLDPGLFVFTRPKGVDVV
                     EGAIF"
     gene            1386..>1775
                     /locus_tag="VFR02_09525"
     CDS             1386..>1775
                     /locus_tag="VFR02_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9870718.1"
                     /translation="MGLGSDLRQARESRKISLETVSQKTRIPVKYLEALESEEYGVFP
                     SQTHARGFARAYAKVVGLDPSALTAQFKAEVKPVEVKINPPNAEAEIPSGFSIPFLKK
                     KGPAGRRERLRDEDFDPEVAAAPEGQAV"
BASE COUNT          333 a          580 c          588 g          274 t
ORIGIN      
        1 ccaaggtggc gaaggcccgg gaggcgggca ccggcgagga tcatccgatg tactacatcg
       61 tggtgctggt ggatgaactg gcggacctca tgatggtggc caaggaccag gtggaggaat
      121 ccatcgcccg gctcgcccag atggcccggg cggtgggcat ccacctggtg ttggcgaccc
      181 aaaggccctc ggtggaggtc atcacgggca tcatcaaggc caatttcccc tcccgcatcg
      241 ctttccaggt cttttccaag gtggattcgc gcaccatcct ggacatgaac ggcgcggaag
      301 cgctgttggg ccgcggggac atgctgtacc tgccttcggg cgcccccaaa cccttgcggc
      361 tccagggggc ctatgttact ttggaagaaa tcgagcggtt ggtcgacttc tggaagtccc
      421 agggccgccc cctctatacc gccgatctga ccacccaaaa atccagggaa ttggtgtccg
      481 agcagagcga ggacgagctc tacgacgagg cggtgcagat catcctgcgg gccaagcagg
      541 cctccacgtc gcacctgcag cgccgcctga aggtgggcta cagccgggcg gcccgccttt
      601 tggacgacat ggaggcccgc ggcatcgtgg ggccggcgga cggcaataag ccccgcaaga
      661 tcctggtgcc cgtcccccag gaccagaccc cggacatgga tgaggagacc gaaggtgaag
      721 cttaagaccc tgaaaaggct ggcggccctg gcggcctgcg gatgggcggc ccccctgctc
      781 ggggcggacg ccccggcccc cttgagcctg gaccagatcc tggacaaggt ggaggcggcc
      841 caggcggcgg cccaggacgt gcagatggac ctgaagacac ggatgcggga caccatgtcc
      901 ggacagaccc aggaggtcca gggcgtggcc atgttgaagg cgcccgacct ggtctacgtc
      961 cactaccaaa aacccaccga gcaattcctc tacatcgacg gcgccctggc ccaaatgtac
     1021 caacccgccc agaagatggt ctatcggcag caggcggccc ccggaagccc ggacgccccc
     1081 gtctacctgg gggtgggccg ggaactgaag cattacgccg acctcagccg ggtctccatc
     1141 gaccatcagt cgggggacga ggtcaccctg ctcttccttc ccaaatcgac ggacgcgggg
     1201 ttcgaccgca tgcgggtgac catccgcaag aaggactggt ggcccgtgcg cctggaggtg
     1261 gagacgcccg cgctggagac ccgctgtgaa ttctccaatt tcaagttcga ccagggcctg
     1321 gatccgggcc tgttcgtgtt cacccggccc aagggtgtgg acgtggtgga aggggcgatc
     1381 ttttaatggg actcggttcg gacctgcgcc aagcccggga atccaggaag atctccctgg
     1441 agacggtctc ccagaagacc cgcatcccgg tcaaatacct ggaggccctg gagtcggaag
     1501 agtacggggt cttcccctcg cagacccacg cgcggggttt cgcccgggcc tacgccaagg
     1561 tggtcggcct ggacccttcg gccctcacgg cgcagttcaa ggccgaggtg aagcccgtgg
     1621 aggtgaagat caaccccccg aacgccgagg cggagatccc ctcgggtttt tccatcccct
     1681 tcctgaagaa aaaagggccg gcgggccgcc gcgaacgcct gcgcgacgag gatttcgacc
     1741 ccgaggtcgc cgcggccccc gagggccagg ccgtc
//
LOCUS       DASTJJ010000788         4917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_352255, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000788 DASTJJ010000000
VERSION     DASTJJ010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352255"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFR02_09530"
     CDS             <1..354
                     /locus_tag="VFR02_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HET9870719.1"
                     /translation="RIDDYANIGGVTPVHQFVRVGKHAMIGGGFRAVQDVAPFMIAGG
                     EPLHIAGINQVGLERAGFAKETIDQLKKAHKTIFRSGLTVKEAVEALARDFPPLEEIK
                     TLIEFISTSQRGLVR"
     gene            358..1161
                     /gene="lpxI"
                     /locus_tag="VFR02_09535"
     CDS             358..1161
                     /gene="lpxI"
                     /locus_tag="VFR02_09535"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET9870720.1"
                     /translation="MREPLGLIAGEGELPLLFARQARRAGYRLCVAALKGAAPASVAG
                     PGDRVEWVSVGQLGKMLAFFKREKVRRAVMHGRVRHASIFSPLGFDWRALALLARVKD
                     RSGPALLAALAGELARAGVRLLDGRFLMKDCLVPRGIWTRVKPTAAQAAACRAGWAQA
                     RALARAGVGQSLLVKNGAVVAVEAMEGTDRAVARAGALAGKGIILIKTAGPRQDWRFD
                     IPTVGPTTLKHLARVRAAGLVVEAGKTFLLQREKTLALAARHGLFLQAV"
     gene            complement(1178..2263)
                     /gene="lptG"
                     /locus_tag="VFR02_09540"
     CDS             complement(1178..2263)
                     /gene="lptG"
                     /locus_tag="VFR02_09540"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HET9870721.1"
                     /translation="MKILSRYILRQFLGPFLLSVFAFSVVILIIQVFDELHYVMQQKP
                     GFWITLEYYLLKIPGLLLQIIPIAVLMGVLFSLGNLSKLSELIAMRAGGVSLYRVAAP
                     LVLCGTAVFLLTLLFDETLVPPADHLSRDIRVNVIERRPPTVNTTRHNLSLMTADNQM
                     IHIGNYNGTTATMTDVIVMGFINGIQLKSRVDAKQVVYRDGQWYFEDGFFRAFDDTGA
                     EITCKPFQEAPFDFQARPADLLKDQTDPAEMNIAQLYANIQQLKQNGEDDHRELVEFH
                     RKIAFPFACVVLSLLGVPWGWSLGKYSGVASSFGICILVAFIYIGGMQIFQTLGSSGA
                     LSPVVSMWAANVLFGLGGLWLLIRKNR"
     gene            complement(2260..3393)
                     /locus_tag="VFR02_09545"
     CDS             complement(2260..3393)
                     /locus_tag="VFR02_09545"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET9870722.1"
                     /translation="MKIAHRYILREIFPSFLISLAVLTFLFIINKVFVLLDMVINKKV
                     PLGDTLVLYFSLVPFVLSLTVPMALMLATLMAFGRLSSDMEITAIKSGGVHLFHLIFP
                     ALLFSVAMTVGMLYFNARILPEANFAFKKIHFKILENQANVAVREKTFIKLFAGYEFY
                     IDRQQAGGLFKDVRVFNRVSPNAPLQTTLAESGWLDTNRKTLQLFFHLDRGVIMWDNV
                     NYHSYDRLYFDRYQICLNLENQLARLSDVKKDYEEMSLSELAAALREPPDPSDPGRSL
                     YLRTEYQKRFALPFACLALTWFCAPLGLWTRSKGFMAFVIGISMIFIYYLLFVLGEVL
                     SQRGQVPPALGLWWANGALTAAGSVLFYLVLFENQAFRFRRRA"
     gene            3691..4833
                     /locus_tag="VFR02_09550"
     CDS             3691..4833
                     /locus_tag="VFR02_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9870723.1"
                     /translation="MPLGWKQFLGLDFAKAYGRSFLKLLLVYNPLWTLGVSVLSGERS
                     PASILFRWGWDLLEASLVCLMGMGVIRAALIVERRWADWSGRDRPKHGMGWYLLFLAF
                     LVPPGLVAALHLMVGLINWLYVGPAIEPRFQWAYYGKEIFGAWLLLLTGFFFLSWQDL
                     RDAVAQGQLKAEEMEKEKLQALLTKLKDQMNPHFLFNTLNTVAALIPEDPVQAERVVV
                     KLSGLFQAVLAASRKTVHPLSEELEFCRDYLEIEKARFGPRLRVDVEAPDQALSVPVP
                     VLILQPLVENAVKHGLSSRAQGGRVWIGARLEGGSLRLWVEDDGVGFGRSTLTGSGSA
                     LENCRKRLELGYGGAARMEIGPRDPSGTRVEMTLPAAAGEPKGASR"
BASE COUNT          839 a         1500 c         1782 g          796 t
ORIGIN      
        1 cgcatcgacg actacgccaa catcgggggc gtgacccccg tgcaccaatt cgtgcgggtg
       61 ggcaagcacg ccatgatcgg cgggggattc cgggccgtgc aggacgtggc ccccttcatg
      121 atcgccgggg gcgagcccct gcatatcgcg ggcatcaacc aggtgggcct ggaacgggcg
      181 ggcttcgcca aggagaccat cgaccaattg aagaaggccc acaagaccat cttccgcagc
      241 ggcctgaccg tgaaggaggc cgtggaggcc ctggcccggg atttcccgcc gctggaggag
      301 atcaagaccc tcatcgaatt catctccacc tcccagcgcg ggctcgtccg ctaggccatg
      361 cgggaacccc tggggttgat cgcgggcgag ggcgagttgc ccctgctctt cgcccggcag
      421 gcccgccgcg cgggataccg cctttgcgtc gcggcgctca aaggcgcggc cccggcctcg
      481 gtggcgggac cgggcgaccg cgtggagtgg gtctcggtcg gccaattggg gaaaatgctg
      541 gctttcttca aacgcgaaaa ggtccgccgg gcggtcatgc acggccgggt gcgccacgcc
      601 tccatttttt cacccttggg attcgactgg cgggcgctgg ccctattggc ccgcgtgaag
      661 gaccggtccg gcccggccct gctcgcggcc ttggccgggg aactggcccg cgccggcgtg
      721 cggttgttgg acggccggtt cctgatgaag gactgcctgg tcccccgggg gatctggacc
      781 cgggtgaagc ccaccgcggc ccaggccgcc gcctgccggg ccggatgggc ccaagcccgg
      841 gccctggccc gcgcgggggt ggggcagagc ctgctggtca agaacggcgc cgtggtggcg
      901 gtggaggcca tggaaggtac ggaccgggcg gtggcccgcg cgggcgcctt ggcggggaag
      961 gggatcatcc tgatcaagac cgccgggccc cgccaagatt ggcgcttcga catccccacg
     1021 gtggggccca cgaccctgaa gcacttggcc cgggtccggg ccgccgggtt ggtggtggag
     1081 gcgggaaaga ccttcctgct gcagcgggaa aagacattgg ccctggccgc ccggcacggg
     1141 cttttccttc aggccgtctg acaggccctg tcccgcctca acggttcttg cggatgagca
     1201 gccaaaggcc ccccaggccg aagagcacgt tggcggccca catggacacc acgggcgaga
     1261 gcgcgcccga actccccaag gtctggaaga tctgcatgcc cccgatgtag atgaaggcca
     1321 ccaggatgca gatgccgaag ctggacgcca ccccgctgta tttccccagg ctccaacccc
     1381 agggcacgcc caagagcgac agcaccacgc aggcgaaggg gaacgcgatc ttccggtgga
     1441 attcgaccag ttcccggtgg tcgtcctcgc cgttctgctt gagctgctgg atgttggcgt
     1501 agagctgggc gatgttcatc tcggccgggt cggtctggtc cttcaacagg tccgcggggc
     1561 gggcctggaa gtcgaagggc gcctcctgga agggcttgca ggtgatctcg gccccggtgt
     1621 cgtcgaaggc ccggaagaac ccgtcctcga aataccactg gccgtcccgg taaacgacct
     1681 gcttggcgtc gacccgggac ttgagctgga tgccgttgat gaaacccatc acgatcacgt
     1741 cggtcatggt ggcggtggtc ccgttgtagt tcccgatgtg gatcatctgg ttgtccgcgg
     1801 tcatgaggga caggttgtgg cgggtggtgt tgacggtggg cggccggcgc tcgatcacgt
     1861 tcacccggat gtcgcgggac aaatggtcgg ccggcggcac cagggtctcg tcgaagagga
     1921 gggtcagcag gaagacggcc gtcccgcaga gcaccagggg ggccgccacc cggtagaggc
     1981 tcacgccccc cgcgcgcatg gcgatgagct cgctgagctt gctcaggttg cccagggaaa
     2041 agagcacccc catgagcacg gcgatcggga tgatctggag cagcagcccc gggatcttca
     2101 acaagtaata ttccagcgtg atccaaaagc cgggtttctg ctgcatgacg taatgcagct
     2161 cgtcgaagac ctggatgatg aggatgacca ccgagaaggc gaagaccgag agcaggaagg
     2221 gccccaggaa ctgccgcagg atgtagcggg agaggatctt cacgcgcgcc tcctgaaccg
     2281 gaaggcctgg ttctcgaaca ggaccaggta gaagaggacc gagccggcgg ccgtcagggc
     2341 gccgttcgcc caccacagcc ccaaggcggg ggggacctgc ccgcgctggc tcaagacctc
     2401 ccccaggacg aagagcaggt aatagatgaa gatcatggag atcccgatca cgaaggccat
     2461 gaagcccttg gagcgggtcc agagccccag gggcgcgcag aaccaggtca gggccaggca
     2521 ggcgaagggc agggcgaagc gcttttggta ctcggtcctc aggtagaggg accggccggg
     2581 gtccgagggg tccgggggtt ctcggagggc ggcggccagc tcggacaggc tcatttcctc
     2641 gtagtccttc ttcacgtcgg acaggcgggc caactggttc tccaggttga ggcagatctg
     2701 gtaccggtcg aaatagaggc ggtcgtagct gtggtaattg acgttgtccc acatgatgac
     2761 gccccggtcc aggtggaaga acagctggag ggtcttccgg ttcgtgtcca gccaaccgct
     2821 ctcggccagc gtggtctgca ggggcgcgtt gggcgagacc cggttgaaga cccggacgtc
     2881 cttgaacagc cccccggcct gctggcggtc gatgtagaac tcataaccgg cgaagagctt
     2941 gatgaaggtc ttttcccgca cggccacgtt ggcctggttc tccaggatct tgaagtggat
     3001 cttcttgaag gcgaaattgg cctcgggcag gatgcgggcg ttgaaataga gcatgcccac
     3061 ggtcatggcc acggaaaaga gcagggcggg gaagatgagg tggaagaggt gcacgccccc
     3121 gctcttgatg gcggtgatct ccatgtcgga gctcagccgg ccgaaggcca tcagggtggc
     3181 cagcatgagc gccatgggga cggtcaggga aagcacgaag gggaccaggc tgaagtaaag
     3241 caccagggtg tcccccaggg gcaccttctt gttgatgacc atgtccagca gcacgaagac
     3301 cttgttgatg atgaacagga aggtcaggac ggccagggag atcaggaacg aggggaagat
     3361 ttcccgaagg atgtagcgat gggcgatttt caatggggcg tccgtcgggt catttcggcc
     3421 gagtataaca caggccccgg gatgggccaa cggcggcggt cccggcgccc cggggggctt
     3481 ttccggcggt tggcgccccg ggacgggcgc cggaagcccg gcggtggcag gatgggggcc
     3541 ggaggcggcg acatggaacg gcgcgggaca gtgacggcga atattgatat catcggaaag
     3601 ccgtacggac aggtttgggc ccggccggac accggggcgc cggtttcccg gcgggacggg
     3661 cccgtccggg accaggaaaa gggaggggcc atgcccctgg ggtggaaaca attcctcggg
     3721 ttggatttcg ccaaggccta cggccgttcc ttcctgaaac tgctgttggt ctacaacccc
     3781 ctctggaccc tgggggtgag cgtcctgtcg ggcgagcgca gcccggcctc catcctgttc
     3841 cgctggggtt gggacctgct ggaggccagc ctggtgtgcc tcatgggcat gggggtgatc
     3901 cgggcggccc tgatcgtcga gcggcgctgg gcggactggt cgggccggga ccggccgaag
     3961 cacggcatgg gttggtacct gctcttcctg gccttcctgg tgccgcccgg cctggtggcg
     4021 gccctccacc tcatggtggg gctgatcaac tggctctacg tgggccccgc catcgagccc
     4081 cggttccaat gggcctatta cgggaaggag atcttcggcg cctggctgct gctgctcacg
     4141 gggttcttct tcctgtcctg gcaggacctg cgcgacgccg tcgcccaggg acagctgaag
     4201 gcggaggaga tggaaaagga aaaactccag gccctgctca ccaagctcaa ggaccagatg
     4261 aacccgcatt tcctcttcaa taccctcaac accgtggcgg cgctcatccc cgaggacccg
     4321 gtccaggccg agcgggtggt ggtgaagctc tcgggccttt tccaggccgt gttggccgcc
     4381 agccgcaaga ccgtccatcc gctttccgag gaattggaat tctgccggga ttacctggag
     4441 atcgagaagg cgcgtttcgg gccccgcctg cgggtggacg tcgaggcgcc cgaccaggcc
     4501 ctgtcggtcc ccgtgcccgt gctcatcctg cagcccctgg tggagaacgc cgtcaagcac
     4561 ggcctgtcct cccgcgccca gggcggccgg gtctggatcg gcgcccggct ggaaggcggg
     4621 agcctgcggc tgtgggtcga ggacgacggc gtggggttcg ggcggtccac cctcacgggt
     4681 tcgggctcgg ccctggagaa ttgccgcaag cgcctggagc tgggctatgg gggcgccgcc
     4741 cggatggaga tcggtccccg ggacccctcg gggacgaggg tggaaatgac gctgccggcc
     4801 gcggcgggcg aacccaaggg ggcatcacga tgatccgggt cctggtggtg gacgacgagg
     4861 cgcccgcgag ggcgaggttg gtgcgcatgc tcgcgggctt cgagggcctg acggtgg
//
LOCUS       DASTJJ010000789         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_231518, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000789 DASTJJ010000000
VERSION     DASTJJ010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231518"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFR02_09555"
     CDS             complement(<1..651)
                     /locus_tag="VFR02_09555"
                     /EC_number="1.17.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/ferredoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET9870724.1"
                     /translation="MAEMFYEIQRRKTRPVMVGKVQVGGGAPVSIQSMTNTHTYDVQE
                     TIDQIKRLEDAGCEIIRVTVESPKSLKVLGQIKAKMNVPLVADIHFAYTIALGAIDEG
                     ADKIRINPGNIGGMDRLAQVVEKCKKYNVAMRVGVNSGSLEKDLLEKHGHPTPEAIVE
                     SAMRHIKFIESFDYHNMIISVKSSHVPTMIRSYRLLAQQCDYPFHVGVTEAGTKMQG"
     gene            complement(757..>1243)
                     /locus_tag="VFR02_09560"
     CDS             complement(757..>1243)
                     /locus_tag="VFR02_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870725.1"
                     /translation="VTLTPAFSYTPTDTPSPTQTPTKTVTPTATRTRTPTPTPTASPT
                     PTPSPSPTASPTMSPSPTDTPVSSVLIPFPNPSDGVTPISFYYNVDQPETAVTLKVFT
                     VASRRIFEDGLSTLPGQHSYTLDWKARGLNPANGLYYFLVEFRDNGQVTHQVMKVLVL
                     R"
BASE COUNT          192 a          345 c          452 g          254 t
ORIGIN      
        1 gccctgcatc ttggtgccgg cctcggtcac gcccacgtgg aagggatagt cgcactgctg
       61 ggccaacaac cgataggacc ggatcatggt gggcacgtgc gagctcttca ccgagatgat
      121 catattgtgg tagtcgaagg attcgatgaa cttgatgtgc cgcatggccg attccacgat
      181 ggcctcgggg gtggggtgcc cgtgcttttc cagcaggtcc ttctccagcg agcccgaatt
      241 gaccccgacg cgcatggcca cgttgtactt tttgcacttc tccaccacct gggccagccg
      301 gtccatgccg ccgatgttgc ccgggttgat gcggatcttg tccgcgccct cgtcgatggc
      361 gcccagggcg atggtgtagg cgaaatggat gtcggcgacc aacggcacgt tcatcttggc
      421 cttgatctgg ccgagcacct tcagcgactt ggggctttcc accgtcaccc ggatgatctc
      481 gcagcccgcg tcctccaggc gcttgatctg gtcgatggtc tcctgcacgt cgtaggtgtg
      541 ggtgttggtc atggactgga tggagacggg cgcgccgcct cccacctgca ccttgcccac
      601 catgaccggg cgggtcttgc ggcgttggat ctcatagaac atctcggcca tcgaaaggct
      661 ccttggaagg tctgggggtt cattatggaa taggtccggg acggtttcaa gccgcccggc
      721 gggcgcggcg gcgccctgaa atggcgggtt ttggccttac cggaggacca acaccttcat
      781 gacctggtgg gtcacctggc cgttgtcccg gaattccacc aggaaatagt agaggccgtt
      841 ggcggggttg aggcccctgg ccttccaatc cagcgtatag gaatgctggc ccggcagggt
      901 ggaaaggccg tcctcgaaga tccggcggga agccaccgtg aagaccttca acgtgacggc
      961 ggtctcgggc tggtccacgt tgtaatagaa ggagatgggc gtgaccccgt ccgaggggtt
     1021 cgggaaaggg atgagcaccg aactcaccgg ggtgtccgtg gggctggggc tcatggtggg
     1081 cgaggcggtc gggctggggc tgggcgtggg ggtcggactg gccgtggggg tcggggtggg
     1141 cgttcgggtg cgggtggcgg tcggggtcac ggtcttcgtg ggcgtctggg tggggctggg
     1201 cgtatccgtg ggcgtgtagg aaaaggccgg cgtcaaggtg acc
//
LOCUS       DASTJJ010000790         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1086799, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000790 DASTJJ010000000
VERSION     DASTJJ010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086799"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VFR02_09565"
     rRNA            <1..1375
                     /locus_tag="VFR02_09565"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          283 a          462 c          499 g          222 t
ORIGIN      
        1 acagtcaaag cgacgttgct gagtccacgc cggccagaaa agcctcgctg ctagtaccgc
       61 tggtgcccgt accgcaaacc gacacaggtg ggcggcggta agtaccgtga ggtgttcgag
      121 ataaccttcg ttaaggaact ctgcaatcta gctccgtaac ttcgggagaa ggagcaccac
      181 tgcgcgtgaa ggagcttgcc tccggagcgc ccggtggtcg cagtgagcgg gcccaggcga
      241 ctgtttaaca aaaacacagg tctctgcgaa cgcggaagcg gacgtatagg ggctgactcc
      301 tgcccagtgc cggacggtca aggggggggc tttcaaacgg cccgaaccga agccccggta
      361 aacggcggcc gtaactctaa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg
      421 acccgcacga aaggagtaac gacttgggcg ctgtcttggc aggggacccg gcgaaactgc
      481 aggccccgtg aagatgcggg gtacccgcga caggacaaaa agaccccgtg gagctttact
      541 gcacgttggc attggcccgg gccgcgggct gcgtaggata ggtgggagcc gttgaacccg
      601 ggctcgtggg tccgggggag gcgacggtga aataccactc tgcgcgcggc cttggcctaa
      661 ccgtgggctt accccccgcg ggaccgtgcc agccgggcag tttcactggg gcggtggcct
      721 cccaaagtgt aacggaggcg cgcaatggtc ccctcaggcg ggatggacat ccgtcagggc
      781 gtgcaacggc gcgaaggggg cttgactgcg agccggacag ggcgagcagg gacgaaagtc
      841 ggccgtagtg atcctacgcc cccgcgtggc aggggcgtgg cttaacggat aaaagctacc
      901 ccggggataa caggcttatc ttgcccaagc gttcccagcg acggcaaggt ttggcacctc
      961 gatgtcggct cgtcgcaacc tggggctgga gtcggtccca agggttgggc tgttcgccca
     1021 ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac agttcggtct ctatccgtcg
     1081 cgggcgcacg gacgcttgcg cggggccgcc cccagtacga gaggaccggg gcggacgggc
     1141 cgctggtgca ccggtcgcgc cgcctggcgc gccgccgggt agccacgccc ggtcgggaga
     1201 agcgctgaaa gcatctaagt gcgaaacccg ccgcaagatg aggcgtccag actccggggg
     1261 gcaacccctg ggagtgaagg ccccaggcag attacctggt tgctaggccg cacgtggacg
     1321 gcgcgcgagc gcctcgtccc agccaagcgg tcctaatggc cgtccccttg cccctcaccc
     1381 ccgtcacacg acacccccca ccaccaacaa ccacccaagg ggctgcccac tgggcaagcc
     1441 cgcgctcgtc cgcttccccc ctgcca
//
LOCUS       DASTJJ010000791         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_596145, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000791 DASTJJ010000000
VERSION     DASTJJ010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596145"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFR02_09570"
     CDS             <1..812
                     /locus_tag="VFR02_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HET9870726.1"
                     /translation="GVGKSTLLGMVGKNTTADINVIALVGERGREVRDFLERDLGPEG
                     LSRSVVVVATSDQSSLLRIQAALVATTIAEYFRDKGRKVLLMMDSITRFAMAQREVGL
                     AIGEPPTTKGYTPSVFAVLPKLLERAGNTDHAGSITGLYTVLVEADDINEPVADACRA
                     ILDGHAVLSRDLAVRNHYPALDVLNSVSRVMNDIVAPDHKRAAVKVRTLLASLKGVED
                     LLAIGAYVKGTQPGVDEALQRRDEVEAFLRQGQDEASTFDETRARLAGFLA"
     gene            821..>1114
                     /locus_tag="VFR02_09575"
     CDS             821..>1114
                     /locus_tag="VFR02_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870727.1"
                     /translation="MARAFAFRLEQVLNYRRQLEEAAIRRMAAAQSRLSGIREEQGRR
                     RAQKDELADWFSGEERRGSFQVEEAAGYTDYLEFLSGLEKGLRERETRTLTEVE"
BASE COUNT          204 a          302 c          427 g          181 t
ORIGIN      
        1 ccggggtggg caagagcacc ttgctcggca tggtggggaa gaacacgacg gcggacatca
       61 acgtgatcgc cttggtgggc gagcgcggac gggaagtccg ggactttttg gagcgggatc
      121 tggggccgga aggcctgtcc cgcagcgtgg tggtggtggc cacatccgac caatcctcct
      181 tgttgaggat ccaggcggcg ttggtggcga cgaccatcgc ggaatatttc cgggataaag
      241 gccggaaagt gctgctgatg atggattcca tcacccgttt cgccatggcg caacgggagg
      301 tggggctggc gatcggcgaa ccgccgacca ccaagggcta caccccctcg gtgttcgcgg
      361 tgctgcccaa gcttttggag cgggcgggga acacggacca cgccgggagc atcaccggcc
      421 tctataccgt gctggtggag gcggacgaca tcaatgagcc cgtggcggac gcctgccggg
      481 cgatcttgga cgggcacgcc gtgctgtcgc gggacttggc ggtgaggaac cactatcccg
      541 cgttggacgt gctgaacagc gtgtcccgcg tgatgaacga catcgtggcc cccgaccaca
      601 agcgggcggc ggtgaaggtg aggacgctcc ttgcttccct caagggagtg gaggacctgc
      661 tggccatcgg ggcctatgtg aaagggaccc agccgggggt ggatgaggcg ctgcaacgcc
      721 gggatgaggt ggaggccttt ttgaggcaag gacaggacga agcctcgacg tttgacgaga
      781 cccgggcccg attggccggg ttcctggctt aagggacgcc atggcgcggg ccttcgcgtt
      841 ccggttggaa caggtgctga attaccggcg ccaattggag gaggcggcca tccggcggat
      901 ggccgcggcc cagtcccggc tgtcggggat ccgggaggag cagggccgcc ggcgggcgca
      961 aaaggatgaa ctggcggatt ggttctccgg cgaagagcgc cggggaagct ttcaggtgga
     1021 agaggccgcg ggttacacgg attacctgga attcctttcg ggcctggaaa agggcctgcg
     1081 ggagagggaa acgaggaccc tgaccgaagt ggaa
//
LOCUS       DASTJJ010000792         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_966215, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000792 DASTJJ010000000
VERSION     DASTJJ010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966215"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1035)
                     /gene="glyA"
                     /locus_tag="VFR02_09580"
     CDS             complement(<1..>1035)
                     /gene="glyA"
                     /locus_tag="VFR02_09580"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9870728.1"
                     /translation="ELIASENFTSLAVMAAQGGVMTNKYAEGYPGNRYYGGCENVDRV
                     EDLARERASRLFFGAEYVNVQPHSGSQANMAVYFTFVQPGDVVLGLDLSHGGHLTHGS
                     KVNFSGKMYNFFAYQTRKEDGRVDLDQARDLARKHKPKMISIGASAYSRDWDYAAFRK
                     IADEVGAFLWADIAHTAGLIAAGELNNPMPHCHVVTTTTHKTLRGPRGGMILMGKDFD
                     NPFGVTAPKSGRVRKMGELLDSQIIPGIQGGPLMHVVAAKAVAFREALDPSFKDYAKR
                     VKANAKALCQALVERGFQIVSGGTDNHLMLIDLRNKKVNGKQAQLALDEAGLTTNKNA
                     VPFDTESPLLT"
BASE COUNT          158 a          337 c          334 g          206 t
ORIGIN      
        1 ggtcaaaagc ggcgattcgg tgtcgaaagg cacggcgttc ttgttggtgg tcagccccgc
       61 ctcatccagg gccaattggg cctgcttgcc gttcaccttc ttgttgcgca ggtcgatgag
      121 catcaggtgg ttgtccgtcc cccccgagac gatctggaag ccccgttcca ccagggcctg
      181 gcagagggcc ttggcgttgg ccttcacccg cttggcgtaa tccttgaagg aagggtccag
      241 cgcctcccgg aaggccaccg ccttggcggc caccacgtgc atcaggggcc cgccctggat
      301 gcccgggatg atctggctgt cgagcagttc ccccatcttg cgcacccggc cgcttttggg
      361 ggccgtgacg ccgaaggggt tgtcgaaatc cttgcccatg aggatcatcc cgccccgggg
      421 accccgcagg gtcttatggg tggtggtggt caccacgtgg cagtggggca tggggttgtt
      481 gagctccccg gcggcgatga gccccgccgt atgggcgatg tccgcccaaa ggaaggcgcc
      541 cacctcgtcg gcgatcttgc ggaaggccgc gtaatcccaa tcccgcgagt aggcggaggc
      601 cccgatgctg atcatcttgg gcttgtgctt gcgcgccagg tcccgggctt ggtccaggtc
      661 cacccggccg tcctccttgc gggtctggta ggcgaagaag ttgtacattt tccccgaaaa
      721 gttcaccttg ctgccgtggg tcaggtgccc gccgtgggag aggtccaacc ccagcaccac
      781 gtcgccgggc tgcacgaagg tgaaatagac cgccatgttg gcctggcttc ccgagtgggg
      841 ctgcacattc acgtattcgg ccccgaagaa aagccgggag gccctttccc gggccaggtc
      901 ctccacccgg tccacgttct cgcaaccccc gtaatagcgg ttgcccggat agccttccgc
      961 gtacttgttg gtcatcacgc cgccctgcgc cgccatgacg gccaggctgg tgaaattttc
     1021 cgaggcgatc agttc
//
LOCUS       DASTJJ010000793         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1576866, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000793 DASTJJ010000000
VERSION     DASTJJ010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576866"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFR02_09585"
     CDS             complement(<1..537)
                     /locus_tag="VFR02_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9870729.1"
                     /translation="MEKPVTILDFQAKRNAGEKITLVTAYDYSMALAADAAGVDAILV
                     GDSLSMTMLGRDSTLSATLEEMAHHCKAVVRGVKRALVIGDMPFLSYQLGPEQALENA
                     GWLMADTGVRAVKLEWCPQAPGVTEFLVGNGVPVMGHVGFTPQHIHQLGGYHAQGKDK
                     RSAQRLLAEAKALESAGAF"
     gene            complement(730..>1130)
                     /locus_tag="VFR02_09590"
     CDS             complement(730..>1130)
                     /locus_tag="VFR02_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET9870730.1"
                     /translation="VSDYIFAKDRLFANLTLDDNELKLYDQIHKILNSQVLKPDLVVY
                     LQASNEVLLKRIAMRGRKFESHISPEYLTELNQAYNYYFFHYQDTPLMVVNTNEIDFV
                     HRDADLDDLLAHMDKVTKGTLYYQPVSVKR"
BASE COUNT          193 a          326 c          370 g          241 t
ORIGIN      
        1 gaaggccccc gcggactcca gggccttggc ttccgccagc agccgctggg ccgaacgctt
       61 gtccttgccc tgggcgtggt agccgcccaa ctggtggatg tgctggggcg tgaagcccac
      121 gtggcccatg acgggcacgc cgttgcccac caggaactcg gtcacgccgg gggcctgggg
      181 gcaccattcg agcttcaccg cccgcacgcc cgtgtcggcc atgagccagc ccgcgttctc
      241 cagcgcctgc tcggggccca gttggtagga caggaagggc atgtcgccga tgaccagggc
      301 ccgcttcacg ccacggacca cggccttgca atggtgggcc atctcctcca gcgtggcgga
      361 cagggtggaa tcgcgcccca gcatggtcat ggacaggctg tcgcccacca ggatggcgtc
      421 cacgcccgcc gcgtcggcgg ccagggccat ggaatagtcg taggcggtga ccagggtgat
      481 cttctccccg gcgttgcgtt tggcctggaa gtccaggatg gtgacgggct tttccatttt
      541 aattcctttg aacaaaaact cgcatcacca ctaaggcacc aaggcaccaa ggaaagcgaa
      601 aatgatggat gtctttcatg gtctgttttt acctaaattt tatttcggtt cacttggtgt
      661 ctgcccccac aacggttttg tgggaatggg ccgccctcgg gcgaggtgcc ttgatggtga
      721 agatccgcct catcttttca cgctgaccgg ctggtaatag agggtgcctt tggtgacctt
      781 gtccatgtgg gccagcaggt cgtccaggtc ggcgtcccgg tgcacgaagt cgatctcgtt
      841 ggtgttcacc accatgaggg gcgtgtcctg gtagtggaag aaatagtagt tgtaggcctg
      901 gttcaactcg gtcaggtact cgggcgagat gtgggattcg aacttgcggc cccgcatggc
      961 gatgcgcttc aagagcacct cgttggaggc ctgcaggtac accaccaggt cgggcttcag
     1021 gacctggctg ttgaggatct tgtggatctg gtcgtagagc ttgagctcgt tgtcgtccag
     1081 ggtcaggttg gcgaagagcc ggtccttggc gaagatgtag tcgctgacca
//
LOCUS       DASTJJ010000794         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_353611, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000794 DASTJJ010000000
VERSION     DASTJJ010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353611"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFR02_09595"
     CDS             complement(<1..587)
                     /locus_tag="VFR02_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9870731.1"
                     /translation="MGLLLRMGWRNVLRNRRRTLLTSSVIALGLASLILTDAVIQGML
                     RNMVATATGTFLGEAQVHAEGFRDSQKVEQTVRDLPRVEALLKGDPAVSAFTERTEVL
                     GMVSSAADAESAAVYGIDPQGERPISKIAQAVLRGEYLAGPSDGGILLGEKLAHILRV
                     GPGDRVVVTCARAGTGELEQEMFRVKGIFSFNAAG"
     gene            complement(589..>1309)
                     /locus_tag="VFR02_09600"
     CDS             complement(589..>1309)
                     /locus_tag="VFR02_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9870732.1"
                     /translation="LVSQGADGSVADDLFTVAGILQAGGQEGADHHLYLRLADAQSFL
                     VLGGRVHEVAVLLKDIRRARPEAQALAGKLGDPDLSVEPWQVVEKDFYKAMTVDKEGD
                     YFSQLIFLVLVGLGVLNTVLMNVLERVPEYGLLKALGTRSSTLTAMILWEMLLLSLFS
                     LVPGAALAWASNAWLAAEGLRLPHPYSYGGMVFSTMYGQVSWPVFLEPAALIVLVAVL
                     VCLPAALRIRRLTPIEALRRA"
BASE COUNT          228 a          464 c          431 g          186 t
ORIGIN      
        1 atgcccgcgg cgttgaacga gaagatcccc ttcacccgga acatttcctg ctccaactcc
       61 cccgtcccag cccgggcgca ggtgaccacc acccggtctc ccgggcccac ccgcaggata
      121 tgggccagtt tctcgcccaa caggatgccg ccgtccgacg ggcccgccag gtattccccc
      181 cgcaagaccg cctgggcgat cttggagatc ggccgctcgc cttgcgggtc aatgccgtag
      241 accgccgccg attcggcgtc ggccgcggaa ctcaccatgc ccaagacctc cgtcctttcg
      301 gtgaaggccg agaccgccgg gtcgcccttc agcagcgcct ccacccgggg caggtcccgg
      361 accgtctgtt cgaccttttg ggagtcccgg aacccctcgg cgtgcacctg ggcctcgcct
      421 aaaaaggtgc cggtggccgt ggccaccatg ttgcggagca tgccctggat gacggcgtcg
      481 gtgaggatga ggctggccag ccccagcgcg atgaccgagg aggtcaggag cgtgcgccgc
      541 cggttgcgca gcacgttgcg ccatcccatg cgcagcaaca ggcccatgtc aggccctcct
      601 caaggcttcg atgggcgtca gacgccggat gcgcagggcc gccggcaggc agaccaggac
      661 cgccaccagg acgatgaggg ccgccggttc caggaagacc ggccaggaga cctggccgta
      721 catggtggaa aagaccatgc cgccgtaact gtagggatgg gggagccgga ggccctcggc
      781 ggccagccag gcgttggagg cccaggccag ggccgccccg ggaacgaggc tgaagagcga
      841 aagcaggagc atttcccaaa ggatcatggc cgtcagggtg gaggaccgcg tgcccagggc
      901 cttcaggagc ccgtactcgg gcacccgttc caacacgttc atcaggaccg tgttcagcac
      961 gcccaggccc accagcacga ggaagatgag ctgggagaaa taatcccctt ccttatccac
     1021 cgtcatggcc ttgtagaagt ccttttccac cacctgccag ggctccaccg aaaggtccgg
     1081 gtcgccgagc ttcccggcga gggcttgggc ctccgggcgg gcccggcgga tgtccttcaa
     1141 gagcaccgcc acctcgtgga cgcgcccccc caggacgagg aagctttggg cgtcggccag
     1201 gcgcaggtag agatggtggt ccgccccctc ctggcccccc gcctggagga tgcccgccac
     1261 cgtgaagagg tcgtcggcca ccgagccgtc cgcgccctgg gagaccagg
//
LOCUS       DASTJJ010000795         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_596827, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000795 DASTJJ010000000
VERSION     DASTJJ010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596827"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFR02_09605"
     CDS             <1..826
                     /locus_tag="VFR02_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870733.1"
                     /translation="TSTFTPTGTLTPPDTATPTVTDTPVISRSGLALLPTAYSNGLFT
                     SDGLNWDLSFSYFIGSIAEHPKGEAGTDFLTPLRLWLLSSDLKYGWVDENGDMPALAS
                     GALLSLLISGNSSSAGSSGSSPGQGASSFQLSGTSMGGFYTVASKRLDKDSALHMGMV
                     FGLQQPFSQLGLGGFAPALNYGDLLPMFTDKLSGDLGHPAEYLFYTGFNTRLLGTKVR
                     VELWKPFPLGGNPVLLNTKVDGLFAFNLAYEKWDEGYALLGYFSFRFTIIPPPVDY"
     gene            834..>1986
                     /gene="lpxB"
                     /locus_tag="VFR02_09610"
     CDS             834..>1986
                     /gene="lpxB"
                     /locus_tag="VFR02_09610"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET9870734.1"
                     /translation="MARPGKRIMVVAGDPSGDLHAARVAAALRRQDPSARLFGMGGPA
                     LAAAGADIRDDLTRPAVMGFVEVVKHLPLLKRRFALCEKWLAEEKPDLLWLVDYPGFN
                     LRLAKKAHALGVPVCYYIAPQVWAWHRERLALMKKVIGKLLVILPFEREFFRKEGMKA
                     VYVGNPLLEEVPARGPSRLKALKKAGLSPAAFPLISAMPGSRKAEVERIWPLYLAAAR
                     RLRSSYPDIAFLVPKPKSLAPEDYPGLLPDDPFYFLETPAFDARRACDLAWVKSGTST
                     LETALLGTPQVLVYKVAALTGWLAKRLLKIRYVGLVNLLAPKPQVPELLQERATPEAL
                     VEETERILGQPKAREAQLKSFAAVKKGLATPRPSSSVTAREILKFLREGA"
BASE COUNT          316 a          686 c          624 g          360 t
ORIGIN      
        1 cacctccacc ttcaccccca cgggcaccct gacgccgccc gacaccgcca cccccacggt
       61 gaccgacacc ccggtcattt cccgttcggg gctggccctg ctgcccaccg cctattcgaa
      121 cgggcttttc accagcgacg gcctgaactg ggacctgagc ttttcctatt tcatcggttc
      181 catcgccgag catccaaaag gcgaggccgg caccgacttc ctcacgcccc tgcgcctgtg
      241 gcttttgtcc agcgacctca agtacggctg ggtggacgag aacggggaca tgcccgccct
      301 ggcctcgggg gcccttttgt cgctgctcat ctcgggcaac agctcatcgg cgggttcctc
      361 ggggtcctcg ccggggcagg gcgcctccag cttccagctt tcgggcactt ccatgggggg
      421 cttctacacc gtggccagca agcgcttgga caaggattcg gccctgcaca tggggatggt
      481 attcggcctg cagcagccct tctcccaatt gggattgggg ggcttcgccc ccgcgctcaa
      541 ttacggcgac ctgttgccca tgttcaccga caagctttcc ggggacctgg ggcacccggc
      601 cgagtatctt ttctataccg gcttcaacac ccgcctgctg ggcaccaagg tgcgtgtgga
      661 gctctggaaa cccttccccc tgggggggaa tccggtactc ttgaacacca aggtcgacgg
      721 gcttttcgcc ttcaacctgg cctacgagaa gtgggacgag ggatacgcct tgctcggtta
      781 tttcagcttc cgcttcacca tcatcccccc gccggtcgat tactaggccc gccatggcaa
      841 gacccggaaa gcggatcatg gtggtggccg gggacccctc gggggacctg cacgccgccc
      901 gggtggcggc ggccctgcgc cggcaggacc cgtcggcgcg cctcttcggc atgggcggcc
      961 ccgccctggc ggcggcgggc gccgacatcc gtgacgacct gacccggccc gccgtcatgg
     1021 gcttcgtcga ggtggtcaag cacctgcccc tgctcaaacg ccggttcgcc ttgtgcgaaa
     1081 aatggctggc ggaggaaaaa ccggacctcc tctggctggt ggactatccg ggcttcaacc
     1141 tgcgcttggc gaaaaaggcc catgccctgg gcgtgccggt ctgctactac atcgcccccc
     1201 aggtatgggc ctggcaccgg gaacgcctgg ccctcatgaa gaaggtcatc gggaaattgc
     1261 tcgtcatcct gcccttcgag cgggaatttt tccggaaaga agggatgaag gccgtctatg
     1321 tggggaaccc cttgttggaa gaggtcccgg cccgggggcc ttcgcgcctg aaggcactca
     1381 aaaaagccgg gttgtccccg gcggcttttc cgctcatcag cgccatgccg ggcagccgca
     1441 aggccgaggt cgagcggatc tggccgctct acctggcggc ggcccgccgc ctgaggtcct
     1501 catatcccga tatcgccttc ctggtgccga aacccaagtc cctggccccg gaggattatc
     1561 cgggcctctt gccggacgac cccttttatt tcctggagac gccggccttt gacgcccggc
     1621 gcgcctgcga cctggcttgg gtcaagtcgg ggaccagcac gctggagacg gccctcttgg
     1681 gcacgcccca agtgctggtc tataaggtgg cggccttgac cggctggctg gccaagcgcc
     1741 tgctcaagat ccgatacgtg ggcctggtga acctgctggc gcccaagccc caggtgcccg
     1801 aattgctcca ggaaagggcc accccggagg cgctggtgga agagaccgag aggatcctgg
     1861 ggcagcccaa ggcgcgggag gcccaactga agtccttcgc cgcggtcaaa aaggggctgg
     1921 ccacgccccg gccttcctcc agcgtgaccg cgcgggagat cctcaagttc ctccgggagg
     1981 gagcgt
//
LOCUS       DASTJJ010000796         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_841713, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000796 DASTJJ010000000
VERSION     DASTJJ010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841713"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..765
                     /locus_tag="VFR02_09615"
     CDS             166..765
                     /locus_tag="VFR02_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HET9870735.1"
                     /translation="MTRTTFFMLACLACAAGTWADSLYPIAGGGNSIYTEKRARRVGD
                     VVTVIIQEQTRASQGVTNNNQKNANVGFGAGVGFFSGYNNIPTQSQVGVGAQSYNQGQ
                     GSASRASQVTGQITAKIISVLPNGNYQISGDSYVEVNEDKQTIELTGEIRPDDISSEN
                     TIPSTRIANVKVKFTGSGPASESAHPGLLTRVLSWLGLF"
     gene            809..>1830
                     /locus_tag="VFR02_09620"
     CDS             809..>1830
                     /locus_tag="VFR02_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET9870736.1"
                     /translation="MKKKTILAVGLSLFLAAPALQAAQVRLKSIANVKGVRENQLIGY
                     GLVVGLQQTGDTQRVTATLQSITNMLSQFGIVVSPAQMRTKNVAAVMVTAQLPALVKS
                     GDKIDVTVSSIGDAQSLQGGNLLLTPLQGPDGQVYAAAQGPLAVGGYSDNTDEFPLVQ
                     TSKTNVGRVPNGALVEREVPSSYVDDNTIAILLTKPDFGQASMVVQALNQQFGEAVAK
                     ASDAGEVDVKIPFNYQDNVVDFVAAVENTQVMADSPTDKVVVNERTGTVVLGQDVRID
                     AVAIAHGNLRLVVRSTTQITHDAAFGKDEMSHTTTITTDNADKKGNLVVLPDGATLMD
                     VVTAINA"
BASE COUNT          372 a          575 c          602 g          281 t
ORIGIN      
        1 acggtgaagg ccctgtcgga cggcaagacc ggggacgtga tccgggtgga gaacaccgac
       61 acccacaagt tgttgaaggc cagggtccag gatgaacgga ccgtgttgat ccaggcgggg
      121 caaccctaag ggggccgccg cccgagcttt tggaggacgg aagcgatgac gagaacgacc
      181 ttcttcatgt tggcctgcct ggcctgcgcg gcgggcacct gggcggattc cctctacccg
      241 atcgcggggg gcggcaattc catctatacc gagaaacgcg cccgccgggt gggcgacgtg
      301 gtgacggtca tcatccagga acagacccgc gcttcccagg gcgtcaccaa caacaaccag
      361 aagaacgcca atgtgggttt cggggcggga gtgggttttt tcagcggcta taacaacatc
      421 ccgacccaga gccaggtggg ggtgggcgcc cagagctaca accagggcca gggttcggcc
      481 agccgggctt cccaggtcac cggccagatc accgccaaga tcatttcggt gctgcccaac
      541 ggcaattacc agatcagcgg cgacagctac gtggaagtga acgaggacaa acagaccatc
      601 gagctcaccg gggagatccg acccgacgac atcagcagcg agaacaccat cccttccacg
      661 cgcatcgcca acgtcaaggt caagttcacg gggagcgggc ccgcctccga gtccgcccat
      721 ccggggcttt tgacgcgggt cctgtcgtgg ttggggttgt tttaacgccg ggcggttccg
      781 gccgaaatct gcggtttgag gtggggacgt gaagaaaaag acaatcctgg cggtcggtct
      841 ttccctgttc ctggcggccc cggcgctcca agcggcccag gtccggttga agtccatcgc
      901 gaacgtgaag ggcgtgcggg aaaaccagct gatcggctac ggcttggtgg tggggttgca
      961 gcagacgggg gacacccaac gggtcaccgc caccctgcag agcatcacca acatgctgtc
     1021 ccaattcggc atcgtggtca gccccgccca aatgcgcacc aagaacgtgg ccgccgtcat
     1081 ggtgacggcc cagctgccgg ccctggtcaa gtccggcgat aagatcgacg tgaccgtgtc
     1141 ctccatcggg gacgcccaga gcctccaggg gggcaacctg ctcttgacgc ccctgcaggg
     1201 ccctgacggc caggtctacg ccgcggccca gggtcccctg gcggtgggcg gatattcgga
     1261 caataccgac gagttccccc tggtgcagac cagcaagacc aatgtgggcc gggtccccaa
     1321 cggcgccctg gtggagcggg aagtgccttc gtcctacgtg gacgacaaca ccatcgccat
     1381 cctgctcacc aagcccgact ttgggcaggc ttccatggtg gtgcaggccc tcaaccaaca
     1441 attcggggag gcggtggcca aggcctcgga cgcgggggaa gtggacgtca agatcccctt
     1501 caactaccag gacaacgtgg tggatttcgt ggccgcggtg gagaacaccc aggtgatggc
     1561 ggattcgccg accgacaaag tggtggtcaa cgagcgcacg ggcacggtgg tgttgggcca
     1621 ggatgtgcgc atcgacgcgg tggccatcgc ccacggcaac ttgcggctgg tggtgcgcag
     1681 caccacccag atcacccacg acgcggcctt cggcaaggat gaaatgagcc acacgaccac
     1741 catcaccacg gacaacgcgg acaagaaggg caacttggtg gtcctgccgg acggcgccac
     1801 cctgatggac gtggtcaccg ccatcaacgc
//
LOCUS       DASTJJ010000797         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_842055, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000797 DASTJJ010000000
VERSION     DASTJJ010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842055"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1536
                     /locus_tag="VFR02_09625"
     CDS             232..1536
                     /locus_tag="VFR02_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET9870737.1"
                     /translation="MRNFRTLWLALGCFIVIASCGLTGCGTKDQGTVIKISSWGDLQE
                     NVILVNLISEFEKLHPDIHVKLERIPFSQYVPKLLTEVVGGIAPDVIFVEVNNFTDLY
                     LRGALEPLTPFIQRDNFPLNDYYPAVVDRFTSNGNTYVIPRDTAPIAVIYYNKQAFDE
                     AHVPYPTDDWDWNEFVSDAKKVMKYDPKDPQRVTRWGFIDEWPIWDAWVYDAGGSFAD
                     DVKKPTQWTFATDPASLVGIQFRSDLMNKYKIMPQPAGLAAMGGVGTADMFENGSAAM
                     YFSGLWKVPQFRQITDFKWDVVMFPKNPSGFRGFPTGGSGYGILKSSKHKEAAWELIK
                     YISGPEGARALASTGLAQPAIMDVANSPLFLDGKVPLNKRMVLDAMKYVKYNPMCKNW
                     FEVHDSIIGPELDRVWNGTESPKEAMDKLRPELAQNPPQTDN"
     gene            1566..>2291
                     /locus_tag="VFR02_09630"
     CDS             1566..>2291
                     /locus_tag="VFR02_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9870738.1"
                     /translation="MPLTSAERKEAISAFLFISPFLVWLLLLMAGPILAAFALSLCQW
                     DGLFAPPKFVGLANYSQMIHDTRFWKALYNTFYFTLLSVPIGMALSVVVALLMNQKVK
                     GIGFFRTVFYLPSVMGASVAVAMLWKWIFDADHGVLNYFISVFSGIPMKDCPGWLASE
                     VWSKPALIIQSFWSVGSGMIIYLAGLQGIPTTLYEAADIDGANKWQQFWTVTLPGLSP
                     VIFFQLIIGIVTSFQIFNQVFIMT"
BASE COUNT          430 a          764 c          658 g          439 t
ORIGIN      
        1 cagcaagagg gccagggtgg cggacttagg ggatgggcgg ctcatggaaa acctccggaa
       61 cggggatgga agagggcgat tttacccccg gcggcgcggg ggcggggggt ttttcacgtt
      121 gacgctcgtt gggccctatg acatacttgt ccggttttcg ggatttttct cgcgcttcgc
      181 caaaacggct tacaaaaaac gattccaatc cgccctgttc caggaggttt tgtgaggaac
      241 tttcgcacgc tttggttggc cctcggctgc tttatcgtta tagcgtcctg cggcttgacc
      301 ggctgcggca ccaaggacca ggggaccgtc atcaagatct cctcctgggg cgacctgcaa
      361 gagaacgtca tcctggtcaa cctgatctcc gaattcgaga agctccatcc cgacatccac
      421 gtcaagctgg aacgcatccc cttcagccag tacgtgccca agctgctgac cgaagtggtg
      481 ggcggcatcg cgcccgacgt catcttcgtc gaggtcaaca acttcaccga cctgtacctg
      541 cggggcgccc tggagcccct cacgcccttc atccagcgcg acaatttccc cctgaacgac
      601 tattacccgg ccgtggtgga ccgtttcacc tccaacggca acacctacgt catcccgcgc
      661 gacacggcgc ccatcgcggt catctattac aacaagcagg ccttcgacga ggcccacgtg
      721 ccctacccga ccgacgactg ggactggaat gagttcgttt ccgacgccaa gaaggtgatg
      781 aaatacgacc ccaaggaccc ccagcgggtg acccggtggg gcttcatcga cgagtggccc
      841 atctgggacg cctgggtcta tgacgcgggc ggcagcttcg ccgacgacgt gaagaaaccc
      901 acccaatgga ccttcgccac cgaccccgcc tccctggtgg gcatccagtt ccgttcggac
      961 ctgatgaaca agtacaagat catgccccag cccgccggtt tggcggccat gggcggcgtg
     1021 ggcacggccg acatgttcga gaacggctcg gcggccatgt acttctccgg cctctggaag
     1081 gtcccccagt tccgccagat caccgacttc aagtgggacg tggtcatgtt ccccaagaac
     1141 ccctcgggct tccggggctt ccccacgggc ggttcgggct acggcatcct gaagagctcc
     1201 aagcacaagg aagcggcctg ggagctgatc aagtacatct cggggccgga aggcgcccgg
     1261 gcgctggcct ccacgggcct ggcccagccg gccatcatgg acgtggcgaa ttcgccgctc
     1321 ttcctcgacg gcaaggtgcc gctcaacaag cggatggtgc tggacgccat gaagtacgtc
     1381 aaatacaacc ccatgtgcaa gaactggttc gaggtgcatg acagcatcat cggtcccgaa
     1441 ctggaccggg tgtggaacgg cacggagagc ccgaaggaag ccatggacaa gctccggccc
     1501 gagcttgcgc agaacccgcc ccagaccgac aattaacccc cgatccctct aaaggagaca
     1561 cgcccatgcc actgacctcc gccgaacgga aggaagccat cagcgccttc cttttcatct
     1621 cgcccttcct ggtgtggctc ctgctcctga tggccgggcc catcctggcg gccttcgccc
     1681 tgtccctgtg ccagtgggac ggcctcttcg ccccgcccaa gttcgtcggg ctggccaact
     1741 acagccagat gatccatgac acccgtttct ggaaggccct ctacaacacc ttctatttca
     1801 ccctgttgtc cgtgcccatc ggcatggccc tgtcggtggt ggtggccctg ctgatgaacc
     1861 aaaaggtcaa gggcatcggc tttttccgca cggtcttcta cctgccctcg gtcatgggcg
     1921 ccagcgtggc cgtggccatg ctgtggaaat ggatcttcga cgccgaccac ggcgtgttga
     1981 actacttcat ctcggtcttc tccggcatcc ccatgaagga ctgcccgggt tggctggcca
     2041 gcgaggtctg gagcaagccg gccctcatca tccagagctt ctggagcgtg ggatcgggca
     2101 tgatcatcta cctggccggg ctgcagggca tccccaccac cctctacgag gccgccgaca
     2161 tcgacggggc caacaagtgg cagcagttct ggacggtcac gctcccgggc ctgtcgccgg
     2221 tcatcttctt ccagctcatc atcggcatcg tgacctcctt ccagatcttc aaccaggtct
     2281 tcatcatgac c
//
LOCUS       DASTJJ010000798         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_719931, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000798 DASTJJ010000000
VERSION     DASTJJ010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719931"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFR02_09635"
     CDS             complement(<1..523)
                     /locus_tag="VFR02_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870739.1"
                     /translation="MMSPLAVRAAGNVAAAPSNYEMVEAPTAYLLPHGGYDLVMRMYE
                     DGGLYLRGNIGFKDILMFGFSGNATNVVGTGDIQVQAPGLFFKLKLLDERGSPLALAL
                     AYDGRGYGTQSGGRFFPGLQKGFYAVVSKELPQAGFLQLHGGVNAVTFDNFDTKKDLG
                     LFGGISFALLSSLV"
     gene            complement(634..1437)
                     /gene="murI"
                     /locus_tag="VFR02_09640"
     CDS             complement(634..1437)
                     /gene="murI"
                     /locus_tag="VFR02_09640"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9870740.1"
                     /translation="MRSRPIGVFDSGLGGLTVVQAIHRELPREKLAYFGDTARIPYGT
                     KAPETIIRYSRQIVRFLLEREKVKAVVVACNTSSAWALPTLRREFKVPILGVVEPGAA
                     TAVSVTRNGRIGVIGTEGTIASKAYPAAIRALAPKARVFAQACPLFVPLVEEGKVAGP
                     LAEAVAREYLRPLLRARVDTLLLGCTHYPLLRRTLSKVAGKRVRIVDSAEETARSLHR
                     NLEARGIELAGHGGGTYYVSDLSRKFKAQAQRFLGRGVPKVEKVFIEKY"
     gene            complement(1475..2443)
                     /locus_tag="VFR02_09645"
     CDS             complement(1475..2443)
                     /locus_tag="VFR02_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET9870741.1"
                     /translation="MAAFPGGAVPADLKARVLAAADAAAARPPDARGKIHVEGTLPHQ
                     GIYDESLASLQDMEAMRDLGLGWRLTGKPDYARACGSYYGAWLAHYHPGFNPIDETRF
                     DNFLIGYDLAAGALGPGLWDKMRAFEKGMAEGYLAGKNITMEGTRTNNWQSHRVKLAA
                     LASFALGDEALIARSREAYQKQLGVNLEADGAVLDFKERDALHYTVYDLEPLAVAALA
                     AKAHGQDWYHLTGASGASLEKALEWLAPYAEGKKTHLEFVHSTIAFDAQRRAAGVEGF
                     GGAWEPGGAAYLYALAARLDPRWEPLSRQLGGAPDWLECLMPLPGK"
     gene            complement(2533..>3758)
                     /gene="cimA"
                     /locus_tag="VFR02_09650"
     CDS             complement(2533..>3758)
                     /gene="cimA"
                     /locus_tag="VFR02_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET9870742.1"
                     /translation="LIQDSVKFLKSRVDEMFYDAEHFFDGYRANPEYALKCLEAALAG
                     GADCLVLCDTNGGSLPEDVEAVVTRVHQRFPGAALGIHCHNDGDLAVANSHAAVAAGA
                     TQVQGCLNGYGERCGNTNLTSLIPNLALKYKTAFVRPLKLERLTETSRLVDEICNMHP
                     RDNAPYVGKSAFAHKAGVHIAAVSKAAATYEHIDPAKVGNKRNILMSDQAGLAVVLHK
                     ARGQDLKMGKDKSEARAVIEQVKRMESEGYQFEGAEASFDLLLKKALGRHRKFFELVG
                     FRVIVENRNEGALVSEATIKVKVNGELENTVGEGDGPINALDQALRKALTKFYPALKE
                     VHLTDFKVRVLDAKDGTAAKVRVLIESRDRKDSWGTVGVSENIIEASWEALVDSIDYK
                     LLKDSGSKPLRRRKP"
BASE COUNT          615 a         1260 c         1246 g          637 t
ORIGIN      
        1 agaccaggga ggagagcagc gcgaaggaaa tgccgccgaa cagccccagg tccttcttcg
       61 tgtcgaaatt gtcgaaggtg acggcgttca cgccgccgtg cagttggagg aagccggcct
      121 gcggcaattc cttgctgacc acggcgtaaa aacccttttg gaggccaggg aagaaccgcc
      181 cccccgactg ggtcccgtaa ccgcggccgt cataggccag ggccagcgcc agggggctcc
      241 cccgctcgtc caacagcttc agtttgaaga acaaccccgg ggcctggacc tggatgtcgc
      301 ccgtccccac cacgttggtg gcgttgcccg agaagccgaa catcaggatg tccttgaacc
      361 cgatgttgcc gcgcaggtag aggccgccgt cctcgtacat gcgcatgacc aggtcgtagc
      421 ccccatgggg gagcaggtag gccgtggggg cctccaccat ctcatagttg gagggggcgg
      481 ccgccacgtt gcccgcggcc cgcacggcca ggggggacat caagcaaagg agcaggaatg
      541 gaattctcag gggatgaacg ggaggcctcc ggttttcttc gattttaggc ccgaaccccc
      601 gaggaaacaa cccgaaaggg gatatccggg ccctcaatac ttttcaatga acactttttc
      661 caccttcggg acgccgcggc ccaggaaccg ctgggcctgg gccttgaact tccgcgacag
      721 gtcgctgacg taataggtgc cgccgccgtg gccggccagc tcgatgcccc gggcctccag
      781 gttgcggtgg aggctgcggg ccgtttcctc ggccgaatcc acgatgcgca cccgcttgcc
      841 cgccaccttg gagagggtcc ggcgcagcaa gggataatgg gtgcagccca aaagcagggt
      901 gtccacccgg gcgcgcagca agggacgcag gtattcccgg gccaccgcct cggccagggg
      961 gccggccacc ttgccctcct ccaccaacgg cacgaacagc gggcaggctt gggcgaagac
     1021 acgggccttg ggggccaggg cccggatagc cgccggatag gccttggagg cgatggtgcc
     1081 ttcggtgccg atgaccccga tgcggccgtt gcgggtgacg gacaccgcgg tggcggcccc
     1141 gggttcgacc acgcccagga tgggcacctt gaattcccgg cgcagggtgg gcagggccca
     1201 ggccgaggag gtgttgcagg ccaccaccac ggccttcacc ttttcccgtt ccagcaggaa
     1261 ccgcacgatc tgccgggaat agcggatgat ggtctcgggc gccttggtgc cgtaggggat
     1321 gcgggccgtg tcgccgaaat aggccagttt ctcccggggc agttcccggt ggatggcctg
     1381 caccacggtc aaaccgccga gccccgagtc gaacacgccg atgggtctgg atctcaaggg
     1441 tcgctcctca tcatggtcca tccttgcgaa cggattattt tcccggcaag ggcatcaggc
     1501 attccagcca atcgggcgcg ccgcccagtt ggcgcgacag gggctcccag cgggggtcca
     1561 accgggccgc cagggcgtaa agataagccg cgcccccggg ctcccaagcc ccgccaaaac
     1621 cttccacccc cgccgcgcgc cgttgggcgt cgaaggcgat ggtggaatgg acgaattcca
     1681 ggtgggtctt tttcccttcc gcgtaggggg ccagccactc cagggccttt tccagcgagg
     1741 cgccgctggc ccccgtcagg tggtaccaat cctgcccatg ggccttcgcc gccagggccg
     1801 ccaccgccag gggttccagg tcatagaccg tgtaatgcag ggcgtcccgt tccttgaaat
     1861 ccaggacggc gccgtcggcc tccaggttca cccccaattg cttttggtag gcttcccggg
     1921 aacgggcgat gagcgcctca tcccccagcg cgaaggaggc cagggcggcc agcttgaccc
     1981 ggtggctctg ccagttgttg gtccgggtcc cttccatggt gatgttcttc cccgccaggt
     2041 agccttccgc catccccttc tcgaaggccc gcatcttatc ccagaggccc gggcccaggg
     2101 ccccggccgc caggtcgtaa ccgatcagga aattgtcgaa acgggtctcg tcgatgggat
     2161 tgaagccggg atggtaatgg gcgagccagg ccccgtaata ggaaccgcag gcccgggcgt
     2221 aatccggctt gcccgtgagg cgccagccca ggcccaggtc ccgcatggcc tccatgtcct
     2281 ggagcgaggc caggctctca tcataaatgc cctggtgcgg gagcgtgccc tccacgtgga
     2341 tcttcccccg cgcgtccggg ggacgggccg ccgccgcgtc cgccgcggcc agcacgcggg
     2401 ccttgaggtc cgccgggacg gccccgcccg ggaaggccgc catggggtcc atgaagacaa
     2461 agggggtcgg atgggccatg gcgggggaaa aacctcccag gaggatggca aggatgggcc
     2521 cggcggtccg cctcaaggct tgcggcgccg gaggggtttg gaaccgctgt ccttcaacag
     2581 cttgtagtcg atggaatcga ccaaggcctc ccaggaagcc tcgatgatgt tctccgagac
     2641 gcccacggtc ccccaactgt ccttgcggtc ccggctctcg atgagcaccc gcaccttggc
     2701 ggcggtgccg tccttggcgt ccagcacccg caccttgaag tcggtcaggt ggacctcctt
     2761 gagcgcggga tagaacttgg tgagcgcctt gcgcagcgcc tggtccaggg cgttgatggg
     2821 gccgtcgccc tcgcccacgg tgttctccaa ctcgccgttg accttcacct tgatggtggc
     2881 ctcggacacc agggccccct cattgcggtt ctccacgatg acccggaagc ccaccagctc
     2941 gaagaacttc cggtggcgtc ccagggcctt cttcaagagc aggtcgaagg aagcctcggc
     3001 cccctcgaat tggtacccct cgctctccat gcgcttgacc tgctcgatga ccgcccgggc
     3061 ctcgctcttg tccttgccca tcttcaggtc ctggccccgg gccttgtgca acaccaccgc
     3121 caggcccgcc tggtccgaca tgaggatgtt gcgcttgttg cccaccttcg cgggatcgat
     3181 gtgctcgtag gtggcggcgg ccttgctcac cgccgcgata tgcacgcccg ccttgtgggc
     3241 gaaggcgctc ttgcccacgt agggggcgtt gtcccgggga tgcatgttgc agatctcgtc
     3301 caccagccgg gaggtctcgg tgaggcgttc caattttaag ggccggacga aggccgtctt
     3361 gtacttgagg gccaggttgg ggatgaggga ggtcaggttg gtgttgccgc agcgctcgcc
     3421 gtagccgttc aggcagccct gcacctgggt ggcccccgcc gcgaccgccg cgtggctgtt
     3481 ggccaccgcc aggtcgccgt cgttgtggca atggatgcca agcgccgccc cgggaaagcg
     3541 ctgatggacc cgcgtcacca ccgcttccac gtcctcgggc agggacccgc cgttggtgtc
     3601 gcaaaggacc aggcagtccg ccccgccggc cagggccgcc tccaggcact tcaaggcgta
     3661 ctcggggttg gcccggtagc catcgaagaa gtgctcggcg tcgtagaaca tctcgtccac
     3721 gcgggacttg aggaacttga ccgaatcctg gatgaggt
//
LOCUS       DASTJJ010000799         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1820712, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000799 DASTJJ010000000
VERSION     DASTJJ010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820712"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..776
                     /locus_tag="VFR02_09655"
     CDS             201..776
                     /locus_tag="VFR02_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9870743.1"
                     /translation="MKAICVYCGSSEGRAPLYRQQARKLGETVARRGLDLVYGGSRHG
                     LMGEVAEGALSMGGRVIGVLPGSLRDKEVAHPGLSALHITQGMHERKAKMADLSDAFV
                     ALPGGFGTLDEIMEMITWNQLKFQAKPIGFLDAGGYFEALFRFMEKASVEGFIQPDLV
                     RALVREGDAESLVQALLNRPWPTLGPWPQGT"
     gene            843..>1003
                     /locus_tag="VFR02_09660"
     CDS             843..>1003
                     /locus_tag="VFR02_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015238863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HET9870744.1"
                     /translation="MSWIDTLKFQEDGLIPVIAQDHKTGEILMFAFANREALEYTAQT
                     GNVTYWSRSR"
BASE COUNT          191 a          301 c          322 g          189 t
ORIGIN      
        1 cgagcatggc aaagtgcccg gcgggcccta tccctcggtg gccctgtcca tcttctggct
       61 catcatggtg tccttgcatt tcttcaagaa aaggccgaag aagcaaccac cggcttctac
      121 gtcgaaatag tccccggcgg aacaatttgt ggcccggcct cgggccggtc cccgttgggg
      181 gaccatgatc gtgggtatgc atgaaagcca tttgcgttta ttgcgggtcg agtgaggggc
      241 gggcgcccct ttatcggcaa caagcccgga agttggggga aaccgtcgcc cggcggggac
      301 tggacctggt ttatggcggg tcccgccacg gtttgatggg ggaagtggcc gagggggccc
      361 tttccatggg cggccgcgtg atcggcgtcc tgcccgggtc cctccgggac aaggaagtcg
      421 cccatccggg gttgagcgcg ctgcacatca cccagggcat gcacgagcgc aaggcgaaga
      481 tggcggacct atccgacgct tttgtggccc tcccgggcgg gttcgggacc ttggatgaga
      541 tcatggaaat gatcacttgg aaccaactca agttccaggc caagcccatc gggttcctgg
      601 atgcgggggg ctacttcgag gccctttttc gattcatgga aaaagcctcc gtggaagggt
      661 tcatccagcc ggacctggtg cgggccttgg tccgggaagg ggacgcggaa agcctggtcc
      721 aggcgctttt aaacaggccc tggccgaccc tgggcccttg gccccaaggg acttaaaaaa
      781 ccctggcttt tcgggcccgg aaagcttaac ttaacccccc tattcccaag ccggagggcg
      841 ccatgagctg gatcgatacc ctgaaattcc aagaggacgg gctcattccc gtcatcgcgc
      901 aggaccataa gaccggcgag atcctgatgt tcgccttcgc caaccgggag gccctggaat
      961 acacggccca gaccggcaac gtcacctatt ggagccgcag ccg
//
LOCUS       DASTJJ010000800         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_720476, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000800 DASTJJ010000000
VERSION     DASTJJ010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720476"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /gene="groL"
                     /locus_tag="VFR02_09665"
     CDS             complement(<1..1319)
                     /gene="groL"
                     /locus_tag="VFR02_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9870745.1"
                     /translation="MMAKQIIYGEEARRALQKGVDKLANAVKVTLGPKGRNVVLDKKF
                     GAPNITKDGVSVAKEIELEDAFENMGAQMVKEVASKTADVAGDGTTTATVLAQAIVRE
                     GLKNVTAGANPMALKRGIDKAVEAVVAELKKIATPTKGKNEIAQVATISANGDKKIGG
                     IIADAMEKVGKDGVITVEEAKGIDTTLDVVEGMQFDQGYLSPYFVTNADRMEVDLDNA
                     YILIHEKKIASMKDLLPVLEKVVQKGRPLLIFAEEVEGEALATLVVNKIRGTFVCAAV
                     KAPGFGDRRKAMLEDIAILTGGKSITEDLGVKLENVKLEDLGQAKKVVIDKDNTTIMQ
                     GSGAKKDLDARCETIRKQIKETTSDYDREKLQERLAKLVGGVAVINVGAATEVEMKEK
                     KARVKQIRGQIAETTSDYDREKLQERLAKLVGGVAVINVGAATETEM"
     gene            complement(1425..1715)
                     /locus_tag="VFR02_09670"
     CDS             complement(1425..1715)
                     /locus_tag="VFR02_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9870746.1"
                     /translation="MKFRPLHDRVLIERVGEAEKTQSGIIIPDAAREKQQKGKIIAAG
                     KGRVNEKGEVLKLDVKEGDTVLFEKYGGEDITIDGKEYVILKEESIVGVVEG"
     gene            complement(1961..2233)
                     /locus_tag="VFR02_09675"
     CDS             complement(1961..2233)
                     /locus_tag="VFR02_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9870747.1"
                     /translation="MTKDELVAKIVETTGIKKADTQKALDALINTIIETIKTGDKVNI
                     TGLGIFKIKDKKARLARNPKTGESIQVPAKKAPKFLPSKNFKEAVK"
     gene            complement(2422..>2606)
                     /locus_tag="VFR02_09680"
     CDS             complement(2422..>2606)
                     /locus_tag="VFR02_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870748.1"
                     /translation="SQWREQADVLIEPDLRLFDLFDFSRGRHLIEMGEKEAKRLMPKI
                     KQVIQRRFYFRPEIYR"
BASE COUNT          410 a          808 c          769 g          619 t
ORIGIN      
        1 ttcatctcgg tctcggtggc cgcgcccacg ttgatcaccg ccacgccgcc cacgagcttc
       61 gccaggcgct cctggagctt ctcgcggtcg tagtcgctgg tggtctcggc gatctggccg
      121 cggatctgct tcacgcgggc cttcttttcc ttcatttcga cctcggtggc cgcgcccacg
      181 ttgatgaccg ccacgccgcc gaccagcttg gccaggcgtt cctgcagctt ctcgcggtcg
      241 tagtcgctgg tggtctcctt gatctgcttg cggatggtct cacagcgggc gtccaggtcc
      301 ttcttggcgc cggaaccctg catgatggtg gtgttgtcct tgtcgatgac cacctttttg
      361 gcctggccca ggtcctcgag cttgacgttc tccagcttga cgcccaggtc ctcggtgatg
      421 gacttgccgc ccgtcaggat ggcgatgtcc tccagcatgg ccttgcggcg gtcgccgaag
      481 cccggggcct tgacggcggc gcacacgaag gtgccgcgga tcttgttcac caccagggtg
      541 gccagggcct cgccctccac ttcctcggcg aagatgagga gcggacggcc cttctggacc
      601 accttctcca gcacgggcag caggtccttc atgctggcga tcttcttctc atggatcagg
      661 atgtaggcgt tgtccaggtc cacttccatg cggtccgcgt tggtgacgaa gtagggggac
      721 aggtagcctt ggtcgaactg catgccctcc accacgtcca gcgtggtgtc gatgcccttg
      781 gcttcctcca cggtgatgac gccgtccttg cccaccttct ccatggcgtc ggcgatgatg
      841 ccgccgatct tcttgtcgcc gttggccgag atggtggcca cctgggcgat ctcgttcttg
      901 cccttggtgg gggtggcgat cttcttgagc tcggccacca cggcctccac ggccttgtcg
      961 atgccgcgct tgagggccat ggggttggcc ccggcggtca cgttcttcag gccctcgcgc
     1021 acgatggcct gggccagcac ggtggcggtg gtggtgccgt cgcccgccac gtcggcggtc
     1081 ttggaggcca cttccttcac catctgggcg cccatgttct caaaggcgtc ctccagctcg
     1141 atctccttgg ccaccgacac gccgtccttg gtgatgttgg gggcgccgaa cttcttgtcc
     1201 agcaccacgt tgcggccctt gggacccagg gtcaccttga ccgcgttggc cagcttgtcc
     1261 acgcccttct gcagcgcgcg gcgggcttct tctccgtaaa tgatctgctt cgccataatg
     1321 ggactcctcc gattttgctt ttcgttctgg tttggggcct cagccccggg ttctcggccg
     1381 ccggacccgg gggtccggca agggcctcaa cggctcgcgg accgtcagcc ttcgaccacg
     1441 ccgacgatgc tctcttcctt caggatcacg tattccttgc cgtcgatcgt gatgtcctcg
     1501 ccgccgtact tctcgaagag gaccgtgtcc ccttccttca cgtccagctt caggacctcg
     1561 cccttttcgt tcacccggcc tttgccggcg gcgatgatct tgcccttctg ctgcttttcg
     1621 cgggccgcgt ccggaatgat gatgccgctt tgggtctttt ccgcttcccc aacgcgctcg
     1681 atcaggaccc ggtcgtgcaa cggacgaaat ttcatggact tcctcctcga tttgatcgcg
     1741 cggaccccag gcccgtcgcg aaggtcaatg tttctttagt gtttgcggca ggaaggccgc
     1801 ttcattagca ctccgatgaa acgagtgcta agggaaggca ataataggca aaggcccggg
     1861 aagcgtcaag gcatggggtc aaataagtgt gtgaacaggg ggtaaaacaa aaagcccccc
     1921 gccggggcgg gaggccgagg ggcttcggaa cgccggaagg ttacttgacg gcttccttga
     1981 agttcttgga cggcaggaac ttgggcgctt ttttggcggg cacctggatg gactcgccgg
     2041 tcttgggatt gcgcgccagc cgggcctttt tgtccttgat cttgaagatg cccagccccg
     2101 tgatgttcac cttgtccccg gtcttgatgg tttcgatgat ggtattgatg agggcgtcca
     2161 acgccttctg ggtgtccgcc ttcttgatcc cggtggtttc cacaattttc gcgaccaatt
     2221 cgtctttggt cattccttca ccccccacaa aatcggaaaa aacccataaa aagcctgggt
     2281 ttgctggctt taagtatgca cctggctgaa aaccctggca agtaaaaagc ctttattttc
     2341 aagggtttga acgttgcaaa tggaaaaaag gcttaaaaaa taagcctttc ttcgaggggc
     2401 cccaaagaaa aaaaagaagg ttcagcggta aatttcaggg cggaaataaa agcggcgctg
     2461 gatgacctgc ttgatcttgg gcatcaggcg tttggcttcc ttttcgccca tctcgatgag
     2521 gtgcctgccc cggctgaaat cgaagaggtc aaaaagcctc aggtccggct cgatgagcac
     2581 atcggcctgt tcccgccact gggact
//
LOCUS       DASTJJ010000801         1398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1454939, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000801 DASTJJ010000000
VERSION     DASTJJ010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454939"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFR02_09685"
     CDS             <1..452
                     /locus_tag="VFR02_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9870749.1"
                     /translation="MLAIDLEKKRISLGLKQVDGDPWDRIEEKYRPGQVLTAKVSRLV
                     EFGAFVELEKNVEGLIHKTEISNPPPKTLEEALKPGDEVTFKVIGVDAGKRRISLSLR
                     ALTNPDSHEELDEEQIVHRKTSAFQKMLKKFMKTAKSAEQEDDEEFE"
     gene            449..>1398
                     /locus_tag="VFR02_09690"
     CDS             449..>1398
                     /locus_tag="VFR02_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870750.1"
                     /translation="MREGALAAFQRFWGPLLGLFLALGVWAPTLGAAGIEPPGLLDQG
                     RALLQARAYELALNRFQLIEKNSDNPGERALALRMVGETQFRNADYASAYQAYQRSLL
                     LTPLSSSRLSLEFKESVSLVYLKKYRSALDKLTALESRAGDKGLLTDLYFWEGECYYQ
                     LGRFDEARREYEKIFATNLHYRYVYLVRYLESWCAFEQKDYAEALRGFGEVGAKTDDP
                     VLRKLSAFQVAEALYRSGRYAEAEKAYAAFADQYPGDRLGAAARYGLGWALEKEKRYA
                     EAVRAFDRVVADAPQDPLAPWAAVREGVDASAAGQTDQAR"
BASE COUNT          264 a          452 c          462 g          220 t
ORIGIN      
        1 agatgctggc catcgacctg gaaaagaagc ggatctcgct ggggctcaag caggtggacg
       61 gcgacccctg ggaccgcatc gaggaaaaat accgccctgg gcaggtgttg accgccaagg
      121 tctcccgcct ggtcgagttc ggggccttcg tggagctgga aaagaacgtc gagggcctga
      181 tccacaagac cgagatctcc aaccctcccc ccaagaccct ggaagaggcg ctcaaacccg
      241 gcgacgaggt gaccttcaag gtcatcggcg tggacgcggg caagcggcgc atctccctga
      301 gcctgcgcgc cttgaccaac ccggattccc acgaggaatt ggacgaggaa cagatcgtcc
      361 accgcaagac cagcgccttc caaaagatgc tcaagaagtt catgaagacg gccaagtccg
      421 ccgagcaaga agacgacgaa gagttcgaat gagggagggg gccttggccg ccttccagcg
      481 tttttggggg cccctcctgg gcctgttcct ggccctcggg gtctgggcgc cgacgctggg
      541 ggcggccggc attgagccgc ccggcctgtt ggaccagggc cgcgccctcc tccaggcccg
      601 ggcctatgaa ttggccctca accgcttcca gctcatcgaa aagaacagcg acaaccccgg
      661 cgaacgggcc ctggcgctgc gcatggtggg cgagacccag ttccgcaacg ccgactacgc
      721 ctccgcctac caggcctacc agcggtcgct gctgctgacg cccctgtcct ccagccggct
      781 gtccctcgaa ttcaaggaat ccgtctcctt ggtctacctc aagaaataca ggtcggccct
      841 ggacaagctc accgccctgg agtcccgggc gggcgacaag ggcctgctga ccgacctcta
      901 tttctgggaa ggcgaatgct attaccagtt gggccgtttc gacgaggccc ggcgggaata
      961 cgagaagatc ttcgccacca acctccacta ccggtatgtc tacctggtgc gctacctgga
     1021 atcctggtgc gccttcgagc agaaggacta tgccgaggcc ctgcggggtt tcggggaggt
     1081 gggggccaag accgacgatc cggtgttgcg gaaactttcc gccttccagg tggcggaggc
     1141 gctttaccga tcgggccggt atgcggaggc cgaaaaggcc tacgcggcct tcgcggacca
     1201 atacccgggg gataggctgg gcgccgcggc ccgctacggc ctgggctggg ccttggaaaa
     1261 ggaaaagcgg tatgccgaag ccgtccgggc ctttgaccgg gtggtggcgg acgcgcccca
     1321 ggatcccttg gccccttggg ccgcggtgcg ggaaggcgtg gacgcctcgg cggcgggcca
     1381 gacggaccaa gcccggca
//
LOCUS       DASTJJ010000802         1888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1454968, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000802 DASTJJ010000000
VERSION     DASTJJ010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454968"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFR02_09695"
     CDS             <1..507
                     /locus_tag="VFR02_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9870751.1"
                     /translation="KSLKAEIAEKRFRDDLYYRLNVVELVVPALRGRREDILPLARLL
                     LAEAALRMKRPVEGLTAPAVDLLLSYPWPGNVRELANAMERAVAVSKSGRVEVGDLPP
                     EVRLVMPLVSEKGVPRFLRDVEKDCILGALEKTGGNRRKAAGLMGMGVATLYRKLKAY
                     KLSDRPKE"
     gene            complement(516..1214)
                     /locus_tag="VFR02_09700"
     CDS             complement(516..1214)
                     /locus_tag="VFR02_09700"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9870752.1"
                     /translation="MITVRRSEERRHIVAGSQNTWMTFDWEDPDDRLRDGFGVLKVFN
                     EEILSPGRGFTLHTHKDMVVVTYVREGVVVYSGPGEEKGLLEPGQFRRMNILTGSRQF
                     TFNSLPDTDSHVFQSGFAPGTGPAAVDGEKKLFTLAERQGVLRLIASPDGKESSLRIQ
                     QDVQIYSTLLHKGNHMIHELKPGRLAWLHVVLGKVLLNGMALQTGDGVGVSEEIAVSF
                     TSQGESEILLFDLP"
     gene            1624..>1888
                     /locus_tag="VFR02_09705"
     CDS             1624..>1888
                     /locus_tag="VFR02_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870753.1"
                     /translation="MKNHWKMAVWLAGFALVTAAPFLVKADGSNFGVKIGDDNEAHYR
                     FRDRRVRHDPQMLQAARLLAAAKERLWYLRDGFNGHRSAAVASI"
BASE COUNT          328 a          551 c          658 g          351 t
ORIGIN      
        1 aaaagcctca aggcggagat cgccgagaag cggttccggg atgatcttta ttaccggttg
       61 aacgtggtgg agctggtcgt ccccgcgttg cgggggcggc gggaggacat cctaccgctg
      121 gcgcggctcc ttttggccga ggcggcgctc cgcatgaaac ggccggtgga aggcctgacg
      181 gcgccggcgg tggacctgct gctgagctat ccttggccgg ggaacgtccg ggaattggcg
      241 aacgccatgg agcgggccgt cgcggtgtcc aagtccggcc gcgtggaggt gggggacctt
      301 ccccccgagg tgcgcttggt gatgcccttg gtgtccgaaa agggggtgcc ccggttcttg
      361 cgggacgtgg agaaggactg catcttgggg gcgttggaga agaccggagg caaccgccgc
      421 aaggccgccg gcctgatggg catgggggtg gccacgcttt accgcaaact caaggcctac
      481 aagttgtccg accgcccgaa ggaataggcc ccgccttagg gcaggtcaaa gagcaggatc
      541 tcgctttcgc cctgggacgt gaaggaaacg gcgatctcct ccgaaacccc caccccatcc
      601 cccgtttgaa gcgccatccc attcagcagg acctttccca gcaccacgtg gagccaggcc
      661 agccgcccgg gcttcaactc atggatcatg tggttcccct tgtggaggag cgtggagtag
      721 atctgaacgt cctgttggat gcgcaaggag gactccttcc cgtcgggtga ggcgatgagg
      781 cgcaaaacgc cctggcgttc cgccagggtg aacagttttt tttccccgtc gaccgccgcc
      841 gggcccgtcc cgggggcgaa cccgctttgg aagacatggg aatcggtgtc cggaagggaa
      901 ttgaaggtga attgccggga accggtgagg atgttcatcc gccggaattg gcctggttcc
      961 aacagcccct tctcttcgcc ggggccgctg tagaccacca cgccttcccg cacataggtg
     1021 accaccacca tgtccttgtg ggtgtggagg gtgaaacccc ggcccggcga gaggatctcc
     1081 tcgttgaaga ccttcaacac gccgaaaccg tcgcgcaggc ggtcgtcggg gtcctcccag
     1141 tcgaaggtca tccaggtgtt ctggcttccg gcgacaatgt ggcggcgttc ctcggacctg
     1201 cggacggtga tcatgatgcg ctcccccggg cccgaaggcc gatttgtccc gatacgggtg
     1261 atggggacca ccgcgcgccg cgtccgggaa cgacccatcc ccgcggcctt ccagaggctg
     1321 gaaggccgtc gatccccttg gaagcggcaa tcttggggga tcttcgccag cttggaccga
     1381 ggggttgttg tgcttcctcc ggggatggga aggttaaagc aagaaccggg ccgtcggttc
     1441 cggggttttt cccgggtttt ggggggttgg ggcggggttg ggggcggaag gaatcggaat
     1501 gaaaggaacg ccgaccgttc tgatagcggt ggacgtcgaa aaccgatttt tccatccgga
     1561 agaagggacg gacagggcac gctccttgct ttgtcctagc tgacgcagcc caaggaggct
     1621 tttgtgaaga accattggaa aatggccgtg tggttggcgg gattcgcctt ggtgacggcg
     1681 gcccccttcc tggtgaaggc ggatgggtcc aatttcggcg tcaagatcgg ggacgacaac
     1741 gaggcgcatt accggttccg ggaccggcgg gtccgccatg acccccaaat gctccaggcg
     1801 gcccggttgt tggccgcggc caaggagcgg ctttggtacc tccgggatgg tttcaacgga
     1861 caccgttcgg ccgccgtggc cagcatca
//
LOCUS       DASTJJ010000803         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_234586, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000803 DASTJJ010000000
VERSION     DASTJJ010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234586"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VFR02_09710"
     CDS             <1..1404
                     /locus_tag="VFR02_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9870754.1"
                     /translation="VRLGQDVFTKGSISRETTQRAVEAFQHFREVLEQHQVARVRAVG
                     TSALREARNRDAFLKKIAQTSGLELRTIGSEEEALLVYLAVSKKIPLEGKTAMLIDIG
                     GGSVEVTLARNAKILATESFKMGAVRLMQVLERKRYGEKKFNLMLQEYVEAARKRFNQ
                     KLAGQKTDICVGTGGNLDALSDLKGQLLKAPDTAFVTLRELDLLIAKLQKLSTEERIL
                     KLRLRPDRADVILPAAIVLREIMKVGRASRVYVPHVGVKEGILIDMVSELLSGKPPLH
                     RDEVMAAARQLGKKYDYDEPHAQQVARLSLDLFEKTKKLHRLGDDNRLLLEAAATVHD
                     IGQFVNYAAHHKHSYYLITSSQIMGLSAHQVELVANIARYHRKAVPLLKHDSYRALDP
                     KDRLLVCKLAAILRIADAMDHEHACKVKDFGAQYKRPHFSVTLRGHDDLLLEKWSLLK
                     KCDLFEKTFKVKFSVRS"
BASE COUNT          314 a          513 c          466 g          226 t
ORIGIN      
        1 gtccgcctgg ggcaggacgt cttcaccaag ggctccatca gccgcgagac cacccaacgc
       61 gccgtggagg ccttccagca tttccgcgag gtcctggagc aacaccaggt ggcccgggtg
      121 cgggcggtgg gcaccagcgc cctgcgggag gcccgcaacc gcgacgcctt cctgaaaaag
      181 atcgcccaga ccagcggctt ggagctgagg accatcggca gcgaggagga agccctgctg
      241 gtgtacctgg cggtctccaa gaagatcccc ctggaaggca agaccgccat gctgatcgat
      301 atcggcggcg gcagcgtgga ggtcaccctg gcccgcaacg ccaagatcct ggccaccgag
      361 agcttcaaga tgggggccgt gcgcctcatg caggtgctgg aacgcaagcg ctacggcgag
      421 aagaaattca acctcatgct ccaagaatac gtggaggccg cccgcaagcg gttcaaccag
      481 aagctggcgg gccaaaagac cgacatctgc gtgggcacgg gcggcaacct ggacgccctg
      541 tccgacctga agggacaact gctcaaggcg cccgacaccg ccttcgtcac cctgcgcgaa
      601 ttggacctgc tcatcgccaa gctccaaaag ctttccaccg aggaacgcat cctcaagctc
      661 cgcctgcgcc ccgaccgcgc cgacgtcatc ctgccggcgg ccatcgtgct gcgcgagatc
      721 atgaaggtgg gccgggcgtc ccgggtctat gtgccccacg tgggcgtgaa ggaaggcatc
      781 ctgatcgaca tggtgtccga gctcttgagc ggcaagcccc ccctgcaccg ggacgaggtc
      841 atggccgccg cccggcagtt ggggaagaaa tacgactacg acgagcccca cgcccagcag
      901 gtggcccggt tgtccctgga cctcttcgag aagacgaaga aactccaccg gttgggcgac
      961 gacaaccgcc tcctgctgga ggccgcggcc accgtgcacg acatcgggca gttcgtcaat
     1021 tacgccgccc accacaagca ttcctactac ctgatcacct cgtcccagat catgggcttg
     1081 agcgcccacc aggtcgaact ggtggccaac atcgcccgct accaccgcaa ggccgtgccg
     1141 ctcctgaagc acgattccta ccgggcgctg gaccccaagg accggctgct ggtctgcaag
     1201 ctggcggcca tcctccggat cgcggacgcc atggaccatg agcacgcctg caaggtcaag
     1261 gatttcggcg cccagtacaa gcgcccgcac ttctccgtca ccctgcgcgg ccacgacgac
     1321 ctgctgctgg aaaaatggtc gctgttgaag aaatgcgacc tgttcgagaa gaccttcaaa
     1381 gtcaaatttt ccgtgaggag ttgatcccat ggccgcgaaa cccaaaccgg gggaagtcga
     1441 aggcaagctc atcgccgtgg agggcctgga cgggtcgggc aaatccaccc agatccgcct
     1501 gttgaagcgc tggctggaa
//
LOCUS       DASTJJ010000804         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_110514, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000804 DASTJJ010000000
VERSION     DASTJJ010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110514"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1652
                     /gene="dnaA"
                     /locus_tag="VFR02_09715"
     CDS             285..1652
                     /gene="dnaA"
                     /locus_tag="VFR02_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9870755.1"
                     /translation="MTVPVDVWGLCLENLKQDLNRQSFETWFKPTKASWLDEKTLQVK
                     VPNEFFRDWIRDHYQPQIRKALRQVHPAVQLEIQFEVESSLPAPAAPVLEVVEKPSLP
                     APIRVSTDSEFILNGKFNFESFVVGNSNRFAHAACLAVAESPARSYNPLFIYGGVGLG
                     KTHLLQAIGNYVVEQNPNIKVLYISSEKFMNDFIQAIQSGRQQDFRNKYRTVDVLLID
                     DIQFWEGKEATQEEFFHTFNVLYEAQKQIVATSDSHPKEIRLEERLRNRFEMGLITDI
                     QEPDLETRIAILRKKAESEGILVPNEVTLFIAHHVKANIRELEGSMLRVIAFASLTRQ
                     EITLELVKEVLKDALPGEERRISVDAIQRLVAERYNCKFSDMKSKKRTKQIAFPRQIA
                     MYLTRELTAHSLTEIGAAFGGKDHTTIIHAYEKIDELCQTDATLGAEVAGLKKHLTDN
                     PSYAF"
BASE COUNT          409 a          608 c          482 g          354 t
ORIGIN      
        1 tttttaaggt ccccaaatac ctggcccgtg ggacacgcgg accggaagag tgaaagccga
       61 ataaaagccc cagaagaccg gcggactacc accggttggg ttatccacag agagtacaca
      121 attgtggata actttttagc caatccttta cacgcttttc tcatcaacgc agcgccggcc
      181 ttcccaaaga cttggggata aaccggggat aaatcagccg gccgacttct tttcccccca
      241 tttgcgggga tttttcggcc tttcaacgtt cgggaggttt gtggatgacc gttccagtgg
      301 atgtctgggg gctctgcctc gaaaacctca aacaagacct gaatcgacag agttttgaga
      361 cctggttcaa gccgaccaag gcctcttggc tcgacgagaa gaccctgcag gtgaaggtcc
      421 ccaatgagtt cttccgggac tggatccgcg accattacca gccccagatc cgcaaggccc
      481 tccggcaggt gcacccggcc gtccagctcg agatccagtt cgaggtcgaa tcctccctcc
      541 ccgcgcccgc ggccccggtc ctggaagtcg tggagaaacc ctccctgccc gcccccatcc
      601 gggtctccac cgattccgaa ttcatcctca acggcaagtt caacttcgag agcttcgtgg
      661 tgggcaacag caaccggttc gcccacgcgg cctgcctggc cgtggccgag tcgccggccc
      721 ggtcctacaa tcccctcttc atttatgggg gggtcggcct gggcaagacc cacctgttgc
      781 aggccatcgg caattacgtg gtcgagcaga accccaacat caaggtcctc tatatctcgt
      841 ccgagaagtt catgaacgac ttcatccagg ccatccaatc gggccggcag caggacttcc
      901 gcaacaagta ccgcacggtg gacgtgctct tgatcgacga catccagttc tgggagggca
      961 aggaagccac ccaggaggag ttcttccata ccttcaacgt gctttacgag gcccaaaagc
     1021 agatcgtggc cacctcggac agccatccga aggagatccg cctggaggaa cgcctgcgca
     1081 accggttcga gatgggcctg atcaccgaca tccaggaacc cgacctggag acccgcatcg
     1141 ccatcctgcg taaaaaggcc gagagcgaag gcatcctggt gcccaacgag gtcaccctct
     1201 tcatcgccca ccacgtgaag gccaacatcc gtgaattgga aggctccatg ctgcgggtca
     1261 tcgctttcgc ctccctcacg cgccaggaga tcaccctgga actggtgaag gaagtgctca
     1321 aggacgcctt gcccggggaa gaacgccgca tttcagtgga cgccatccaa cgcctggtgg
     1381 ccgagcgtta caactgcaaa ttctccgata tgaagtccaa aaaacgcacc aaacagatcg
     1441 cctttccccg ccagatcgcc atgtacctca cccgcgagct caccgcccac tctctcacgg
     1501 agatcggcgc ggctttcggc ggcaaggacc acaccacgat catccacgct tatgagaaga
     1561 tcgacgagct ctgccagacc gacgccacgc tgggcgcgga ggtggcgggc ctgaagaagc
     1621 acctcacgga caatccctcc tacgcttttt aaccgaggct tttccaccgg gttatccccg
     1681 gcctatcccc gttccccccg accacgggag gccagcgcct tcatttccgg gttgaggtcc
     1741 gcttttcccc aaagaaattc acaaagaggc cccttgggcg tggataaacc ccgcttcggg
     1801 gcaaaatcat tgcggacctg tggatttctc ttataatacc cccaatttat tcc
//
LOCUS       DASTJJ010000805         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_478693, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000805 DASTJJ010000000
VERSION     DASTJJ010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478693"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1194
                     /locus_tag="VFR02_09720"
     CDS             <1..>1194
                     /locus_tag="VFR02_09720"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HET9870756.1"
                     /translation="SSQTLIDRIARAGGGDGRVVQAATDGESYGHHTKFGDRSLAYAL
                     AVEAPRRGYWVTNYGAFLEKHPPTQEVEIKRGEDGLGTAWSCAHGVGRWFKDCGCQTG
                     GREGWNQAWRGPLRKAFDYLRDTVAAHFEAERGRLFIDPWAARDAYIDLLLDPGLSRR
                     AFLEKHAGRPLTDLEMVRALTQLEIQRSAMLMYTSCGWFFTELSGIETVQVLKYAAQM
                     FDMLESLGYESPEGPFLEILSEAHSNIHEFGNGADIFRRFAEPSRVAPEGVVAHLAMS
                     SLVDENLPEKAEAGGFRYELEDGRREEHGRFALVTGHVTLEQAMTRQRFRYAFAGLHL
                     GGIDFYGACRPFLRAENFQRSAKKIWGQFYTVSLPKLLRLMQEEFGPQELSVDQLLPE
                     SRQKI"
BASE COUNT          198 a          420 c          396 g          180 t
ORIGIN      
        1 tctccagcca gacgctcatc gaccgcatcg cgcgggccgg cggcggggac ggccgggtgg
       61 tgcaggccgc caccgacggc gagtcctacg gccaccacac caagttcggc gaccgaagcc
      121 tggcctacgc cctggccgtg gaggcgcccc gccggggtta ttgggtgacc aactacggcg
      181 ccttcctcga gaagcacccg cccacccagg aagtggagat caagcggggc gaggacggcc
      241 tgggcacggc ctggagctgc gcccacggcg tgggccgctg gttcaaggat tgcggctgcc
      301 agaccggggg ccgggaaggc tggaaccagg cctggcgggg ccccctgcgc aaggccttcg
      361 actacctccg ggacaccgtg gccgcccatt tcgaggccga acggggccgg ctcttcatcg
      421 acccctgggc cgcccgggac gcctatatcg acctcttgct ggacccaggc ctttcccgcc
      481 gggccttctt ggaaaaacac gccggccgcc ccctgaccga cctggaaatg gtgcgggccc
      541 tgacccaact ggagatccag cgcagcgcca tgctcatgta caccagctgc ggttggttct
      601 tcaccgagct ctcgggcatc gagaccgtac aggtgctcaa gtacgccgcc cagatgttcg
      661 acatgctgga gtcgctgggc tatgagtcgc ccgaaggccc cttcctggag atcctgtcgg
      721 aagcccacag caacatccac gagttcggca acggggccga catcttccgc cgcttcgccg
      781 agcccagccg ggtggcgccc gagggcgtgg tggcccacct ggccatgtcc agcctggtgg
      841 acgagaacct gcccgaaaag gccgaggccg ggggtttccg ctacgagttg gaggacggcc
      901 gccgggaaga gcacggccgt ttcgccctgg tgacgggcca cgtgaccctg gaacaggcca
      961 tgacccgcca gcgtttccgc tacgccttcg cgggcctgca cctgggcggc atcgacttct
     1021 acggggcctg ccggcccttc ctgcgggcgg agaatttcca gcgttcggcc aagaagatct
     1081 ggggccagtt ctacaccgtg tccctgccca agctgcttag gctcatgcag gaggagttcg
     1141 gcccccagga actcagcgtg gaccaactgc tccccgagag ccgccagaag atct
//
LOCUS       DASTJJ010000806         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_843504, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000806 DASTJJ010000000
VERSION     DASTJJ010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843504"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VFR02_09725"
     CDS             complement(<1..1105)
                     /locus_tag="VFR02_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9870757.1"
                     /translation="MKHSRKFAALAAASVLAMTLAACGGGGSGGGGGGNSGGLSGDAA
                     ANLDARGPITYVQGKDNSNVVRPLVDKWNASHPNEKVTFKEQSDKADQQHDDLVQNFQ
                     AKNASYDVVDVDVVWTAEFAAKGWLQPLKGKTAFDTTGFLPATVATATYKDTLYAAPQ
                     TSDGGILYYRKDLVPTPPKTWDEMMGMCPIAKKNNMDCYAGQFAQYEGLTVNAAEAIN
                     TAGGVIVGKDGKPAVNTPEAKAGLNNLVQAFKDGNIPKQAITYQEEQGRQSFEAGKLL
                     FLRNWPYVYNLAKTDGSSVVKDKFGIAPLPGKTGPGASSLGGHNIAMSVYSKHKATAM
                     DFMKCMTSEETEKFYATQGSLAPVREDLYADADL"
BASE COUNT          158 a          359 c          381 g          254 t
ORIGIN      
        1 cgaggtcggc gtcggcatac aggtcctccc ggaccggggc cagcgaaccc tgcgtggcgt
       61 agaacttctc ggtttcctcg ctggtcatgc acttcatgaa gtccatggcc gttgccttgt
      121 gcttgctgta gacgctcatg gcgatgttgt gtccgcccag ggaggaggcg ccggggccgg
      181 tcttgcccgg aagcggggcg atcccgaact tgtccttgac cacggacgag ccgtcggtct
      241 tggccaggtt gtacacgtag ggccagttgc gcaggaacag cagcttgccg gcttcaaacg
      301 actggcggcc ctgctcttcc tggtacgtga tggcctgctt ggggatgttg ccgtccttga
      361 atgcctggac caggttgttc agcccggcct tggcttcggg ggtgttgacg gccggcttgc
      421 cgtccttgcc gacgatgacg ccgccggcgg tgttgatggc ctcggccgcg ttgaccgtca
      481 ggccctcata ctgggcaaac tggcctgcgt agcagtccat gttgttcttc ttggcgatgg
      541 ggcacatgcc catcatctcg tcccaggtct tgggcggggt ggggaccagg tccttgcggt
      601 agtacaggat gccgccgtcg gaggtttgcg gggccgcgta cagcgtgtcc ttgtaggtcg
      661 ccgtggcgac ggtggccggc aggaagccgg tggtgtcaaa ggccgtcttg cccttcagcg
      721 gctgcagcca gcccttggcc gcaaactccg ccgtccagac aacgtccacg tcgacgacgt
      781 cgtagctggc gtttttggcc tggaagttct ggacgaggtc gtcgtgctgc tggtccgcct
      841 tgtccgactg ctccttgaag gtgaccttct cattcgggtg cgacgcgttc cacttgtcca
      901 cgaggggccg gaccacgttc gagttgtcct tgccctggac gtaggtgatg ggtccgcgcg
      961 cgtccaggtt ggcggccgca tcgccgctga ggccgccgga gtttccgccg ccaccgccgc
     1021 cgcttccccc gccgccgcag gcggccaatg tcatcgccag gaccgaggct gccgccaggg
     1081 ctgcaaactt ccttgaatgt ttcattccac gctctttccc gtcagtgaaa acgttcaatc
     1141 atgaagttca at
//
LOCUS       DASTJJ010000807         1866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_110932, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000807 DASTJJ010000000
VERSION     DASTJJ010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110932"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..749
                     /locus_tag="VFR02_09730"
     CDS             282..749
                     /locus_tag="VFR02_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870758.1"
                     /translation="MTLKQDKPMELQNPWTPTRADLAEAFQAALKPFGDLTVMPIDSF
                     NVKASSLFLDRFYRSASTGMALGLRCAQDLAGILSDGTRLEGQALLSPLEALDHLAAR
                     FARQLQDRAQARGAFFPLDWDRRDPDPQGEPAAAGRLLASLSLVEIKLWQTKF"
     gene            767..>1866
                     /locus_tag="VFR02_09735"
     CDS             767..>1866
                     /locus_tag="VFR02_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870759.1"
                     /translation="MKSMKTLVSLLSILVGPMTAAAGSDPYAPQALQALVPAGAKIVQ
                     VTPYDVRGVGQKDAVVFYEASAAPGQPAGCGVVLVSKNLKPLWSFRQTGATHVWVPAG
                     DHPGVVSLDPAHPAVPYLVFNPYFDAVKSQFHVFRWAGKDFAEIRHADLGDDPQVALM
                     PDKVPAVVADNFGYPTPHLFVLKNDRLVPADYQYPHFFDKTVKEAEKSLRDIQMIPHP
                     DYLEVESKYLAAFAYAHRCEEGLRFADKVLNMRPTGLGQDMSSQAAVEIHHQKGCLYL
                     EMGKEAEGMAEFKIASSLDERTEYQGQAGVYAQLGDYYAGKNDGARAVAAYETARNCA
                     PRNDTRVAQSLQGHAQEAAQAAGLGQDALEVSP"
BASE COUNT          351 a          622 c          593 g          300 t
ORIGIN      
        1 gcggatgggt tcgacgtaat tctagcaatt tcccggggtg ggccccaagc gcgaatacgg
       61 aaaagtcccg gcaaatcaag gcgaagcggc gccgtgccat ttttaaggca aaaaaacaag
      121 cttgaatggg taaaaagtac ccaggcgggc attttttcct gcgcacccca cctttcccaa
      181 cccggcttta cccggtcaaa atcacgattt tttttggcac gacagttgcc ttagtcatag
      241 ggcgttggac gatctaagac gaaggattgg ttgaaatgcc cgtgaccctc aagcaagaca
      301 agccgatgga actccaaaac ccctggacgc cgacgcgcgc cgacctggcg gaagcctttc
      361 aggcggccct gaagcccttc ggggacctga ccgtgatgcc gatcgacagc ttcaacgtca
      421 aggcctcgag cctgttcctg gaccgctttt accgctcggc ctccaccggg atggcgctgg
      481 gactgcgctg cgcccaggac ctggcgggca tcctctccga cggcacccgc ctggaaggcc
      541 aggcgctctt gagcccgctg gaggccttgg atcacttggc cgcgcgcttc gcgcggcaac
      601 tgcaggaccg ggcccaggcc cggggagcct ttttccccct ggattgggac cgcagggacc
      661 ccgatcccca aggggagccc gcggccgccg gccgcctgtt ggccagcctg tccctggtgg
      721 agatcaagct gtggcagacg aagttttaag aacaaaggag cccgtcatga agtccatgaa
      781 aacgctggtg agcctcttgt ccatcctggt ggggccgatg acggccgccg ccggatccga
      841 tccctatgcc ccgcaggccc tgcaggcgct ggtgcccgcc ggcgccaaga tcgtccaggt
      901 cacgccctat gacgtccgcg gggtggggca gaaggacgcg gtggtcttct acgaggcgtc
      961 cgcagccccc ggccagcccg cgggttgcgg ggtggtgctg gtctcgaaaa acctgaagcc
     1021 gctgtggagc ttccgccaga ccggcgccac ccacgtctgg gtgcccgcgg gcgaccatcc
     1081 gggcgtggtg tccctggacc cggcccaccc ggcggtgccc tacctggtct tcaaccccta
     1141 ctttgacgcg gtcaagtcgc agttccacgt cttccgctgg gccggcaagg acttcgctga
     1201 gatccggcac gccgacctgg gcgacgaccc ccaggtggcc ctgatgcccg acaaggtgcc
     1261 ggccgtggtg gcggacaact tcggctaccc cacgccccat ctgttcgtgc tcaagaacga
     1321 ccggctggtg ccggcggact accaataccc ccacttcttc gacaagaccg tgaaggaagc
     1381 cgagaagtcc ctgcgcgaca tccagatgat cccccatccg gattacctgg aagtggaatc
     1441 caaatacctg gcggccttcg cctacgcgca ccggtgcgag gaggggctgc ggttcgccga
     1501 caaggtcctc aacatgcgcc ccacgggcct gggccaggac atgagctccc aagcggcggt
     1561 ggagatccac caccagaagg gctgcctgta cctggaaatg ggcaaggagg ccgagggcat
     1621 ggccgagttc aagatcgcct cgagcttgga tgaaaggacc gaatatcagg gccaggcggg
     1681 ggtctacgcc caactgggcg actattacgc cggaaagaac gacggggccc gcgcggtggc
     1741 ggcctatgag acggcccgca actgcgcccc ccgcaacgac acgcgggtgg cccagtccct
     1801 gcagggccat gcccaagagg ccgcccaggc ggcgggattg ggccaggacg ccctggaagt
     1861 ctcccc
//
LOCUS       DASTJJ010000808         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_599064, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000808 DASTJJ010000000
VERSION     DASTJJ010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599064"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /gene="prfB"
                     /locus_tag="VFR02_09740"
     CDS             complement(join(<1..989,991..1062))
                     /gene="prfB"
                     /locus_tag="VFR02_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777923.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9870760.1"
                     /translation="MGLALFELDVKSLERKLTEVRDHLDPAQTRREIEKLEAQTTHPD
                     FWSNTQEAQQVTGRLARLKDSLALVEKFEKHYEDLLACQELLKEQHDPTLQDELKREN
                     DRFEADFREFELKRNFTGKYDFNNAYFSIHPGAGGTESQDWASILLRMYLRWMEEKGY
                     RVETMDYQPADEAGIKSVSLLVQGPWAYGFLKVEKGVHRLVRISPFDAAKRRHTSFAS
                     VDVMPEVEDVEFEIKPDDIQMDTYRSGGKGGQNVNKVETAVRLTHLPTGIIVACQAER
                     SQFKNRDKAMKMLKAQLFQYYQDKQDEEMSKVAGEKKKIEWGSQIRSYVFQPYQLVKD
                     HRTNTEISDIDRVMDGGLDP"
     gene            1284..3692
                     /gene="pheT"
                     /locus_tag="VFR02_09745"
     CDS             1284..3692
                     /gene="pheT"
                     /locus_tag="VFR02_09745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9870761.1"
                     /translation="MLISYNWLRRYLPLPESPETILPRLVQAGVEVGSVRQVGGDITG
                     VVVAELLSVEKHPQADRLSLTQVTTGAETFRVVCGAKNIAPGQKVPLAKLGAVLPGDF
                     KIKPAKIRGEESQGMLCSAKELGLAADAEGILILPADAPLGQDFTAYMGLPDTVFELE
                     ITPNRSDLLSHLGLARELGALLDRPVQLPEAAPKESAPPAASRVKVSIEAPDLCFLYS
                     ARLIEGVKVGPSPHWLSQALERVGQKSINNVVDVTNFVLMETGQPLHAFDLGTLSGGR
                     VAARRAKAGEKIPLLDGTERELKDTMLVIADAEKPVALAGVMGGANSQVKEGTRAVLL
                     EAALFLPGSVRKTARSLSISTDSSYRFERGVDPQGVARALDRAAALILETAGGTLATG
                     VVQQAGSKLFAPAQVRFRPGRCRTLLGTDFNDEEQLGVLRRLGCAVSGQPGPEGYAVT
                     IPGRRVDLEREIDLIEEVARVLGYDRIPLLLPRVPVLFKAPAEPIPFEEPLRSVFHQA
                     GLNEAVNSSFLSRRFPDQLGLPPDDPRRKLQALANPLADDQDVLRSSLLPSLLLNAQA
                     NLARQRDAVALYEINKVFLASPQGVLEKRQAAALLAGETSAAAWSSPARQADFFDLKG
                     LAEGLLRACRLAPPTWDYRGLTAPFHPAQGFRVLDGRGGVLLQGGALHPKALKAYDIH
                     VPCFALELDLSTLAAAPLLPLDYEPLPKFPAAWRDIALVVPDGVTSGQVVEAIRSQGG
                     AELREVLLFDLYRGPHVAPGHRSLAYRLLFQDPERTLTDEEVAGKVSKIVEALKTRYA
                     IALR"
     gene            3679..4005
                     /locus_tag="VFR02_09750"
     CDS             3679..4005
                     /locus_tag="VFR02_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870762.1"
                     /translation="MPSDELFPEVSKPPAGWDGLEKRINRLLESVVQLRNSNGQLMKE
                     NMGLKNQLREAASSPAVDSAETEKLRRDYTRALEDLRKLQKNLAQMEKLTAELRGKKA
                     GEAEAS"
BASE COUNT          664 a         1450 c         1272 g          721 t
ORIGIN      
        1 ggatccagcc cgccgtccat cacccggtcg atatcggaga tctcggtgtt ggtgcggtgg
       61 tccttgacca gctgataggg ctggaagacg taggaccgga tctggcttcc ccattcgatc
      121 ttcttcttct cccccgccac cttggacatt tcctcgtcct gcttgtcctg gtagtactgg
      181 aacagctggg ccttgagcat cttcatggcc ttgtcccggt tcttgaactg ggaccgttcg
      241 gcctggcagg ccacgatgat ccccgtgggc aggtgggtga ggcgcacggc ggtctccacc
      301 ttgttgacgt tctggccgcc cttgccgccg gagcggtagg tgtccatctg gatgtcgtcg
      361 ggcttgatct cgaactccac gtcctccacc tcgggcatca cgtccaccga cgcgaaggag
      421 gtgtggcgcc gcttggcggc gtcgaagggc gagatgcgca ccagccggtg cacgcccttc
      481 tccaccttca ggaagccgta ggcccagggg ccctgcacca acagggagac ggacttgatg
      541 ccggcctcgt ccgcgggctg gtagtccatg gtctccaccc ggtagccctt ttcctccatc
      601 caacgcaggt acatgcgcag caaaatggac gcccagtcct ggctctcggt gccgccggcg
      661 cccgggtgga tggaaaaata ggcgttgttg aagtcgtatt tgcccgtgaa gttgcgtttg
      721 agctcgaact cgcggaaatc cgcctcaaag cggtcgttct cccgcttgag ctcgtcctgc
      781 agggtggggt cgtgctgttc cttgagcagt tcctggcagg ccagcaggtc ctcgtagtgc
      841 ttctcgaact tctccaccag ggccaggctg tccttcaggc gggccaggcg gcccgtgacc
      901 tgctgggctt cctgggtgtt ggaccagaaa tccggatggg tggtctgcgc ctcgagcttc
      961 tctatttccc gtctcgtttg agcggggtca aagatgatcc ctcacttcgg tcaacttacg
     1021 ctccagagac ttgacgtcca attcgaacaa cgccaatccc atcaccgccc tttcggtttt
     1081 aggtgggccc ggcgggagat ccccccgggc ctccggtttc gggcccaatc ttataggggc
     1141 gggccgggaa aaacaccttt taaatgccgg gacccggcgg ggtgcattta gttgacagtc
     1201 ccggggggct cccgtaaact tgcggccaaa ccaaagcaat tcaagggttt tccggggttt
     1261 ccccatcctt ccagggacag gtcatgctca tctcctataa ttggctgcgc cgctaccttc
     1321 cgcttcccga atcccccgaa accatcctgc cccgcctggt ccaggccggg gtggaggtgg
     1381 gatcggtgcg gcaggtgggg ggggacatca ccggcgtggt ggtggccgaa ctgctcagcg
     1441 ttgaaaaaca cccccaggcg gaccggcttt ccctgaccca ggtcaccacg ggcgcggaga
     1501 ccttccgggt ggtctgcggg gccaagaaca tcgcccccgg ccaaaaggtg cccctggcca
     1561 agctgggggc ggtcttgccg ggggacttca agatcaagcc cgccaagatc cgcggcgaag
     1621 aatcccaagg catgctctgt tccgccaagg aactgggcct ggccgccgac gccgagggca
     1681 tcctcatcct ccccgccgac gcccccttgg ggcaggactt caccgcctat atgggcctcc
     1741 ccgacacggt gttcgagttg gagatcaccc ccaaccgttc cgacctcttg agccacttgg
     1801 gcctggcccg tgaactgggc gccctgcttg accgccccgt gcaattgccc gaggccgccc
     1861 caaaggaaag cgcccccccg gcggcttccc gggtgaaggt gtccatcgag gccccggacc
     1921 tctgcttcct ctacagcgcc cgcctgatcg agggcgtgaa ggtgggcccc tcgccccatt
     1981 ggctgtccca ggcgctggaa cgcgtgggcc aaaagtcgat caacaacgtg gtggacgtga
     2041 ccaacttcgt gctgatggaa acgggccaac ccctgcatgc cttcgacctc ggcaccctgt
     2101 cgggcggccg ggtcgccgcg cgccgcgcga aggcgggcga aaagatcccg ctcctggacg
     2161 gcaccgagcg cgagttgaag gacaccatgc tggtcatcgc cgacgccgag aagcccgtgg
     2221 cgctcgcggg cgtcatgggc ggcgccaatt cccaggtcaa ggaagggacc cgggccgtcc
     2281 tgctggaagc cgccctcttc ctgcccggca gcgtgcgcaa gaccgcccgg tcgctttcca
     2341 tctccaccga ctcctcctac cgatttgagc ggggcgtgga cccgcagggc gtggcgcggg
     2401 ccctcgaccg ggccgccgcc ttgatcctgg aaacggccgg cgggacgctg gccacaggcg
     2461 tcgtccagca ggccggctcc aagctcttcg cccccgccca ggtacgtttc cgtcccgggc
     2521 gttgccggac cctcttgggc accgacttca acgacgagga acagttgggc gtgctccgcc
     2581 gcctgggttg cgcggtatcc ggccagccag gccccgaggg ctacgccgtc accatccccg
     2641 gccgccgagt ggacctcgag cgcgagatcg acctgatcga ggaagtggcc cgcgtcctgg
     2701 gctatgaccg catccccttg ttgttgcccc gggtgcccgt gctcttcaag gcccccgccg
     2761 agcccatccc cttcgaggaa cccctgcgct cggtcttcca ccaggcgggc ttgaacgagg
     2821 ccgtgaactc ctcgttcctt tcccgacggt tccccgacca actgggcctg ccgcccgacg
     2881 acccgcgccg gaagcttcag gccctggcca accccctggc ggacgaccag gacgtgctgc
     2941 gctccagcct gctgccttcc ctcttgctca acgcccaagc caacctggcc cggcagcggg
     3001 acgccgtcgc cctttacgag atcaacaagg tcttcctggc ctcgccccaa ggcgtgctgg
     3061 aaaaacgcca ggccgcggcc ctgctggccg gcgagacctc ggcggctgcc tggtcttccc
     3121 ccgcccgcca ggccgacttc ttcgacctga agggcctggc cgaaggcctg ttgcgcgcct
     3181 gccgcctggc acccccgacc tgggactacc ggggcctgac cgcccccttc cacccggccc
     3241 agggcttccg ggtcctggac ggccggggcg gggtgctgtt gcagggcggc gccctgcatc
     3301 ccaaggcgct caaggcctac gacatccacg tgccctgttt tgccctggaa ctggacctgt
     3361 ccaccctggc cgccgccccc ttgctccccc tggactacga gcccctgccc aagttcccgg
     3421 ccgcctggcg ggacattgcc ttggtcgttc ccgatggtgt aacatcgggg caggtcgtgg
     3481 aggccatccg gtcccaaggc ggcgccgaat tgcgggaagt cctcctgttc gacctgtacc
     3541 gggggcccca cgtggccccg ggccaccgca gcctggccta ccgcctgctg ttccaggatc
     3601 ccgaacggac cctgaccgac gaagaagtgg ccggtaaagt ctcgaagatc gtcgaagccc
     3661 tgaaaacgag gtacgcgatt gccctccgat gagctcttcc ccgaggtgtc caagccgccc
     3721 gcgggttggg acggcctgga aaaacgcatc aaccgcctgt tggaaagcgt ggtccagttg
     3781 cgcaactcca acggccagct gatgaaggag aacatgggcc tcaagaacca gctgcgcgag
     3841 gccgcctcct cccccgccgt cgacagcgcc gagaccgaga aactccgccg ggactacacc
     3901 cgggccctgg aggacctgcg caagctccag aagaacctcg cccagatgga aaagctcacc
     3961 gccgaactgc gcggcaagaa ggccggggag gcggaggcgt catgaccgac aaaaagtccg
     4021 tcaccgtgac catctacggc aacgaataca ccctgaaggg cgaggccgac ccccagtaca
     4081 tcctggaact ggcccgcttc gtcgacg
//
LOCUS       DASTJJ010000809         1365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_111291, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000809 DASTJJ010000000
VERSION     DASTJJ010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111291"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFR02_09755"
     CDS             <1..674
                     /locus_tag="VFR02_09755"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET9870763.1"
                     /translation="RRNGWWKPALLALGLAGLIVLDRSFHLVDRLRELHHWIASLGPW
                     GGPAAYTLLFALGTLLALPGSAMTLVAGLFFDVPRAVAVSALGSSLGSSATFLLARTF
                     ARRDLARWASRDARFRKLDRLTERHGAWIVALLRLMPLSPFSVLNYAFGLTRVRFSQY
                     ALWSFLCTLPGTFFFVLSARALAQGVRQHRIPWGSVAALVGVTVLLACLMAWVEKRVM
                     ARPGR"
     gene            788..>1365
                     /locus_tag="VFR02_09760"
     CDS             788..>1365
                     /locus_tag="VFR02_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9870764.1"
                     /translation="MISAQGISLHFGKKVLFDHVTVKFNPGYCYGLIGANGAGKSTFM
                     KILAGQIKPDEGEVVIDRGVKVAMLKQDQYAFDEYSVIDTVLMGREDLWKIQQEKDAL
                     YAKSEMSHDEGLRAAELEAEFGEMDGYTIAAGAGELLEGLGIETAKHEWKMKQLTGGW
                     KVRVLLAQVLYAKPDVLLLDEPTNNLDIKTID"
BASE COUNT          211 a          462 c          453 g          239 t
ORIGIN      
        1 aacggcgcaa cggctggtgg aaacccgccc tcctggcctt ggggctggcg ggcctgatcg
       61 tcctggaccg cagcttccat ctggtggacc gcctgcgcga gctgcaccat tggatcgcgt
      121 ccctggggcc ctggggcggg ccggccgcct acacgctgct cttcgcgttg ggcaccctcc
      181 tggccctgcc cggatcggcc atgaccctgg tggcggggct ttttttcgac gtgccccggg
      241 ccgtggcggt ctcggccctg ggatcctccc tgggcagctc ggccaccttc ctgttggccc
      301 ggaccttcgc gcggcgggac ctggcgcggt gggcctcccg ggacgcgcgg ttccggaaac
      361 tggaccgcct gaccgagcgc cacggcgcct ggatcgtggc cctgctgcgc ctcatgccgc
      421 tttcgccctt cagcgtgctc aactacgcct tcggcctgac ccgggtgcgt ttctcccaat
      481 acgccctttg gtccttcctc tgcaccctgc cggggacctt cttcttcgtg ctcagcgccc
      541 gcgccttggc ccagggcgtg cggcaacacc ggatcccctg ggggtcggtc gcggccctgg
      601 tgggggtgac ggtgttgctg gcctgcctca tggcctgggt ggaaaaacgg gtgatggccc
      661 gaccgggccg gtgacggggc cggatgggcc gggggagccc cccgattcac cccttgacgt
      721 gcccctggaa tcattcgaca attgcccttc ccattccggc ccctgggggc cccgttaaag
      781 gtgttccatg atctccgccc aaggcatttc cctccatttc ggcaaaaagg tgctctttga
      841 ccacgtgacg gtcaagttca acccgggcta ttgctacggg ctcatcgggg ccaacggcgc
      901 gggcaaatcc accttcatga agatcctggc gggccagatc aagcccgacg agggcgaggt
      961 ggtcatcgac cgcggcgtca aggtggccat gctcaagcag gaccaatacg ccttcgacga
     1021 atacagcgtc atcgacaccg tgctcatggg ccgggaggac ctctggaaga tccagcagga
     1081 aaaggacgcc ctttacgcca agagcgagat gagccacgac gagggcctaa gggccgccga
     1141 gctggaggcc gaattcgggg agatggacgg ctacaccatc gcggccgggg ccggggagct
     1201 gttggagggc ctgggcatcg agaccgccaa gcacgaatgg aagatgaagc agctcaccgg
     1261 cggctggaag gtgcgggtgc tgctggccca ggtgctctac gccaagcccg acgtgctgct
     1321 cctggacgaa cccaccaaca acctggacat caagaccatc gactg
//
LOCUS       DASTJJ010000810         1863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_479356, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000810 DASTJJ010000000
VERSION     DASTJJ010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479356"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VFR02_09765"
     CDS             <1..1701
                     /locus_tag="VFR02_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870765.1"
                     /translation="PFGTAGDSGEVSFAITAPQGNGELLAFEITDATTKEPLAVGAVK
                     ADVQPGMVSDLFVTMGTVISPCYTVDTSSMGGNAYVNFDQDQVAASDTVTADLSVSSY
                     PSGSVTYYQFTPQNGAMMAYLGNGDLVDFAAVPDPSAFNTGSSSFLNKGDVYCLQLAG
                     GLYAWVQVADPNEPFGLNPPGPQGPLLRFRVSDQPYYAYYRTASDANSNCTVPPPTPT
                     PIGPTGSTAVNTYAGSGQKGASNGLSLSASFNYPSGVAIDSAGDLFVADTFNNRIRKI
                     DTSGNVSTLAGTGAEGSADGPAGQATFSRPFGIAVNAAGTTVIVADTANSEIRVIDVN
                     NAVVTTLAGAVSQNGYVDSTNFTLSRVDHPVGVAVDPSGTTIFVADTGNHVIRAITSA
                     GVTTYAGSNYSCGAGTCGSEGDVDGAQLGTAEFDGPVGVAVDGSGNVYVSEGKGRLIR
                     KVSGGTVSTLAGCPDEAGYSNGTAGECQGSLFNIPVQLSVDAGGNIYIADPPNNVIRV
                     ISSGFVNTLAGSGTSGYANGPTNVAQFKYPEGVVVNSAGTTVFVVDTNNQRIRSITLP
                     "
BASE COUNT          316 a          665 c          578 g          304 t
ORIGIN      
        1 cccttcggca cggcgggcga ttcgggcgag gtctctttcg ccatcacggc gccccagggc
       61 aacggggaat tgctggcctt cgagatcacg gacgccacga ccaaggagcc cctggccgtg
      121 ggcgcggtga aggccgacgt acagcccggc atggtgagcg acctcttcgt caccatgggc
      181 acggtcatca gcccctgtta caccgtggac acctcatcca tgggcggcaa cgcctacgtc
      241 aatttcgacc aagaccaggt ggccgcctcg gacaccgtca cggccgacct gtcggtatcc
      301 tcctatccct cgggcagcgt gacctattac cagttcacgc cccagaacgg cgccatgatg
      361 gcctacctgg gcaacggcga cctggtcgac ttcgccgccg tgcccgaccc ctccgccttc
      421 aacacggggt cttcctcgtt cctcaacaag ggcgacgtct actgcctgca gctcgcgggc
      481 ggcctttacg cctgggtgca ggtggccgac cccaatgagc ctttcgggtt gaacccgccg
      541 ggcccccagg gtcccttgct gcgctttagg gtcagcgacc agccctatta cgcctattac
      601 cgcaccgcgt cggacgcgaa ttcgaactgc accgttcctc ctcccacccc cacgcccatc
      661 gggcccacgg gttccaccgc cgtgaacacc tacgcgggca gcggccaaaa gggcgcctcc
      721 aacgggcttt ccctctcggc ctccttcaac tatcccagcg gcgtcgccat tgatagcgcc
      781 ggcgacctct tcgtggccga caccttcaac aaccgcatcc gtaagatcga cacctcgggc
      841 aacgtgtcca ccttggcagg tacgggggcc gaaggctccg ccgacggtcc ggcgggccag
      901 gccaccttct cccggccctt cggcatcgcc gtgaacgccg cggggaccac ggtgatcgtg
      961 gccgatacgg ccaacagtga gatccgagtc atcgacgtca acaacgcggt ggtcaccact
     1021 ctggctgggg cggtcagcca gaacggctac gtggacagca cgaacttcac cctgtcccgg
     1081 gtggaccacc cggtcggcgt ggcggtcgat ccttcgggga ccaccatctt cgtggccgac
     1141 acgggcaacc acgtgatccg cgccatcacc tcggcgggcg tgaccaccta cgccggctcg
     1201 aactacagct gcggggccgg cacctgcggt tcggaaggcg atgtggacgg cgcccaactg
     1261 ggcaccgccg agttcgatgg gcccgtgggg gtggcggtgg acggttcggg aaacgtctat
     1321 gtttccgagg gcaagggccg cctgatccgt aaggtctccg gggggacggt ttccacgctg
     1381 gccggctgcc cggatgaggc cggttacagc aacggcaccg ccggcgagtg ccaaggaagc
     1441 ctcttcaaca tccccgtcca gttgtcggtg gatgcgggcg ggaacatcta catcgctgat
     1501 ccgcccaaca acgtcatccg ggtgatctcc tcaggcttcg tgaacaccct ggcgggcagc
     1561 gggacatcgg gctacgccaa cgggcccacc aatgtggccc agttcaagta tccggagggg
     1621 gtcgtggtga attcggcggg gaccacggtc ttcgtggtgg acaccaacaa ccagcggatc
     1681 cgctcgatca cgctcccctg atcggggagc tttcggcccc tccctgccgc atggcgggga
     1741 ggggcttttt tttgccccgg accccctagg acaacccggc gcccaccaaa cggcccaggc
     1801 cgtaagtgac cgcggcggcc aaaagcccca ggcccaattg cctcgcgccc gcgaagagcg
     1861 ggg
//
LOCUS       DASTJJ010000811         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_844320, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000811 DASTJJ010000000
VERSION     DASTJJ010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844320"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1008
                     /locus_tag="VFR02_09770"
     CDS             <1..>1008
                     /locus_tag="VFR02_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9870766.1"
                     /translation="GEPVTAADLFLEWAAGSGYYNDATLDANFKVARGNAYFNYAGDT
                     TGLSQTSMPVIGDNGASLTLTYTKPFSDWETALGSTVSIPAHIVAARVGLKDANALVT
                     LLQGIPKGDPAAPVKANPELRKIANFWNTGFDTKSMPDPSLALSNGPYLVKGITAGKD
                     LVLTRNADYNWGTVPTLDTVTVHYTARPEAQIAALKAGKADIASPALTQDSAAALADA
                     KSGGVQTQVGASLGFDQAVLNFKGVLAKPDFRTAFLKTVPRQDIVDQAAKPVNPGATV
                     LDSFVFRALQTPYKESSASNGTAAYAAADIDASKKLLAGATPTVRVLYNKDDPVRSTE
                     FN"
BASE COUNT          197 a          372 c          290 g          149 t
ORIGIN      
        1 ggcgaacccg tcacggccgc ggacctcttc cttgagtggg ccgccggctc cggctactac
       61 aacgacgcaa ccctggacgc caatttcaag gtggcccgcg gcaacgccta cttcaactat
      121 gcgggggaca ccaccgggct gtcgcaaacg tccatgcccg tcattggcga caacggcgca
      181 tcgctgaccc tgacctacac caagccgttc tccgactggg agacggcact gggttcgacg
      241 gtgagcattc cggcccacat tgtggcagcc cgggtcggcc tgaaggacgc caacgccctc
      301 gtcacgctcc tgcaaggcat cccgaagggt gatccggcag ccccggtcaa ggcaaatccc
      361 gaacttcgca agattgccaa cttttggaac acgggctttg acacaaaatc gatgccggac
      421 ccctccctgg ccttgtccaa cgggccctac ctggtcaagg gcatcaccgc cggcaaggac
      481 ctggtcctca cacgcaacgc cgactacaac tggggcacgg tgcccaccct ggacaccgtc
      541 accgtccact acaccgcccg gcccgaggcg cagatcgcag ccctgaaggc aggcaaggcc
      601 gacatcgcct cgcccgccct gacccaagac agtgcggccg cactcgccga cgcgaagtcc
      661 ggcggcgtcc aaacgcaggt gggtgccagt ctcggctttg accaggccgt gctcaatttc
      721 aagggcgtcc tggccaagcc cgatttccgc accgcgttcc tgaaaacggt gccgcgccag
      781 gacatcgtgg accaggcggc caagccggtc aaccccggcg ccacggtcct ggactccttt
      841 gtgttccggg cactccagac cccgtataag gaatcatcgg ccagcaacgg cacggccgcc
      901 tacgccgccg cggacatcga cgcgtccaag aagctgctgg ccggggcgac gcccacggtc
      961 cgtgtcctct acaacaagga cgatcccgtg cggtccacgg agttcaac
//
LOCUS       DASTJJ010000812         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1335231, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000812 DASTJJ010000000
VERSION     DASTJJ010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335231"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFR02_09775"
     CDS             complement(<1..247)
                     /locus_tag="VFR02_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase"
                     /protein_id="HET9870767.1"
                     /translation="MIHPIPDVLSPQEARELRQRLEQARWIDGKKTAGISAAPTKTNQ
                     ELDQADPAFPGIAQTVLNALAKSRAFASRSLPLSTSTP"
     gene            346..1041
                     /locus_tag="VFR02_09780"
     CDS             346..1041
                     /locus_tag="VFR02_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET9870768.1"
                     /translation="MPTLTCFQHIDCEGPGTLQTVLEPLGFRLNILKPYRGDPVPAEL
                     GDGLVVLGGPMGVYEREQYPWMEAELAAIRRALDASLPTLGVCLGSQMLAHAAGAQVF
                     RGALPEVGWRPIALTPEGKKDALLEGLPGAFDVFHWHGDTFTLPKDAALLAGSDWFPH
                     QIFRLGKAYGFQCHLEVTEAMAREWAHLYAKELAPAGGPTPEARVLGDLPRHAAALGA
                     LAEKVFTRFAGSF"
BASE COUNT          152 a          368 c          358 g          182 t
ORIGIN      
        1 ggggcgtgga ggtcgacagg ggcagcgacc gggacgcgaa ggcccggctc ttggccagcg
       61 cgttcagcac ggtctgggcg atccccggaa aggccggatc ggcctggtcc agctcctggt
      121 tggtcttagt gggggccgcc gaaatgccgg cggtcttctt gccgtcgatc caacgggcct
      181 gctcgagcct ttggcgcagt tcccgggcct cttgggggct caggacgtcg gggatcggat
      241 ggatcatggc ttgctccttg ggaaagatga ggggccccta tccaaagggg tgtccggcgg
      301 attatcttag cagggccgcc cccattctcc cacgggagga gtcccatgcc caccctgacc
      361 tgcttccaac acatcgattg cgagggcccc ggcaccctcc agaccgtcct ggaacccctc
      421 ggtttccgcc tgaacatcct caaaccctac cggggcgacc ccgttccggc ggaactgggc
      481 gacggcctgg tggtgctggg cgggcccatg ggcgtttatg agcgcgagca atatccctgg
      541 atggaggccg agctggccgc catccgccgg gcgctggacg cctccctgcc gaccctgggg
      601 gtctgcttgg gatcccagat gctggcccac gccgcgggcg cccaggtgtt ccggggggcc
      661 ttgcccgagg tgggatggcg gcccatcgcc ctgacgcccg aggggaaaaa ggacgccttg
      721 ctggagggtc tccccggcgc cttcgacgtc ttccactggc acggcgacac cttcacgctt
      781 ccaaaggacg cggcactttt ggcgggcagc gattggttcc cccaccagat cttccggctg
      841 ggcaaggctt acggtttcca atgccacctg gaggtgaccg aggccatggc ccgcgaatgg
      901 gcccatctct acgccaagga actggcgccc gccggtggcc ccacgcccga ggcgagggtc
      961 ctgggggacc ttccccgcca cgccgccgcg ctcggggcgc tcgccgaaaa ggtcttcacc
     1021 cgcttcgccg gatctttttg atccccccag cccgcccatg
//
LOCUS       DASTJJ010000813         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_844729, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000813 DASTJJ010000000
VERSION     DASTJJ010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844729"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VFR02_09785"
     CDS             complement(<1..1425)
                     /locus_tag="VFR02_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9870769.1"
                     /translation="MSQTASAVMTDGHAAEQHHEHTGLWSWIATVDHKRIGLLYLGTA
                     LFFLLTGGIEALMMRIQLARPNNDFVSPELFNQLFTMHGTTMIFMVVMPTLIGFANYF
                     VPLMIGARDMAYPRLNAMAYWFLPFGGLLMHFSWLAGGAPAAGWFSSAPLSETPFNST
                     HGVDYWALGLFVMGIGSISTSINIIATIIACRPPGMTLFRMPLFVWMTFVNSFLITLA
                     MPILNASLVMLFVDRIFSAHFFLPEHGGSAVIWQHFFWAFGHPEVYILVLPAFGMISE
                     VIPVFARRPIFGYGTMVGSVVTIAVLSFGVWAHHMFTVGLGNAADAFFAASTMLIAVP
                     TGIKIFNWGATLWGGSIRLNTAMLFCIAFLIEFTIGGLSGITFAVVPIDWQMNASYYL
                     VAHFHYVIFGGTYFAMMAGIYYWFPKMTGRILSESLGKWHFWATVIGFNMTFFVQHFL
                     GFMGMPRRVYTYPDLPGWATLNMIS"
     gene            complement(1422..2396)
                     /gene="coxB"
                     /locus_tag="VFR02_09790"
     CDS             complement(1422..2396)
                     /gene="coxB"
                     /locus_tag="VFR02_09790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9870770.1"
                     /translation="MNQANVFNPHVATMPVFDPLAAQPAAISHLFITVLWICAGIMVV
                     VCTMVFGALIKYRHKEGAPEPKPYFGNHTVEIIWTVVPSMIVIWLIWLTIDGMGKYYP
                     DLSKKDQADIRVIGHQWWWEAHYKDTGVVVANEIHIPVNKKLYVFLDSADVIHNFWVP
                     QLARKLDAIPNFPNAIYLEAEKPGTYLGACDEYCGVQHAWMRFLVIAQTPEDYAAWVK
                     HQQTAPPPVARLTGSAAHGAKLFHDMTCANCHAINNVPPLVPQTAPDLTHVASRETLG
                     ACILTNTPENLARWLHDPQAIKPGCRMPNLKLDDQQVADLVNYLETLQ"
     gene            complement(2709..>2936)
                     /locus_tag="VFR02_09795"
     CDS             complement(2709..>2936)
                     /locus_tag="VFR02_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET9870771.1"
                     /translation="VSFPLFAKIEVNGPGTHPLYRFLKHAEPDPQGKEDIGWNFTKFL
                     IGRDGRPVARFVSQVTPAELEADVKKELDRP"
BASE COUNT          585 a          775 c         1055 g          521 t
ORIGIN      
        1 ggagatcatg ttgagggtgg cccaacccgg caggtcgggg taggtataga cccggcgcgg
       61 catgcccatg aagcccagga agtgctggac gaagaaggtc atgttgaagc cgatgaccgt
      121 ggcccagaaa tgccacttgc ccagcgactc ggacaggatg cggccggtca tcttggggaa
      181 ccaatagtag atgcccgcca tcatggcgaa gtaggtgccc ccgaagatga cgtagtggaa
      241 gtgcgccacc aggtagtagc tggcgttcat ctgccagtcg atgggcacca cggcgaaggt
      301 gatgccggac agcccgccga tggtgaactc gatgaggaag gcgatgcaga agagcatggc
      361 cgtgttcagg cggatggaac cgccccagag ggtggcgccc cagttgaaga tcttgatgcc
      421 ggtgggcacc gcgatgagca tggtggaggc cgcgaagaag gcgtccgcgg cgttgccgag
      481 ccccacggtg aacatgtggt gggcccagac gccgaagctc aacaccgcga tggtcaccac
      541 cgagcccacc atggtgccgt agccgaagat gggccgccgg gcgaagaccg ggatgacctc
      601 cgagatcatg ccgaaggccg gcaacaccag gatgtagacc tccggatggc cgaaggccca
      661 gaaaaagtgc tgccagatga ccgccgaacc cccgtgttcg ggcaggaaga agtgggccga
      721 gaagatgcgg tccacgaaga gcatgaccag ggaggcgttg aggatgggca tggccagggt
      781 gatgaggaac gagttcacga aggtcatcca cacgaacagg ggcatgcgga acagggtcat
      841 gccgggcggg cggcaggcga tgatggtggc gatgatgttg atggaggtgg agatggaccc
      901 gatgcccatc acgaagaggc ccagggccca atagtcgacc ccgtgggtcg agttgaaggg
      961 cgtctcgctc aagggggcgg aggagaacca gcccgccgcg ggggcgccgc cggccagcca
     1021 gctgaagtgc atcaaaagcc cgccgaaggg caggaaccag taggccatgg cgttgaggcg
     1081 ggggtaggcc atgtcgcggg ccccgatcat caggggcacg aaatagttcg cgaagccgat
     1141 gagcgtgggc atgaccacca tgaagatcat ggtggtgccg tgcatggtga agagctggtt
     1201 gaagagctcg ggggacacga agtcgttgtt gggccgggcc agctggatgc gcatcatcag
     1261 cgcctcgatg ccgccggtca agaggaagaa gagcgccgtg cccaggtaca gcaggccgat
     1321 gcgcttgtgg tcgaccgtgg cgatccagct ccacaatccc gtgtgttcat ggtgctgctc
     1381 ggccgcgtgg ccgtccgtca tgactgcgct ggctgtttgg ctcattgtaa ggtctccagg
     1441 taattgacta agtccgcaac ttgctggtcg tccaacttca agttcggcat gcggcagccg
     1501 ggcttgatgg cctgcgggtc gtgcagccag cgggccaggt tctcgggcgt gttggtcagg
     1561 atgcaggcgc ccagggtctc ccggctggcc acgtgggtca ggtcgggggc cgtctggggc
     1621 accagggggg gcacgttgtt gatggcgtgg caattggcgc aggtcatgtc gtggaacagc
     1681 ttggcgccgt gggcggccga accggtcagg cgggcgacgg ggggcggcgc ggtctgctgg
     1741 tgcttcaccc aggccgcgta gtcctcgggc gtctgggcga tgaccaggaa gcgcatccag
     1801 gcgtgctgga cgccgcaata ttcatcgcag gcgcccaggt aggtgccggg cttctcggct
     1861 tccaggtaga tggcgttggg gaaattgggg atggcgtcca gcttgcgggc caactggggc
     1921 acccagaagt tgtggatgac gtcggccgag tccaggaaaa cgtacaactt tttattcacc
     1981 gggatgtgga tctcattggc caccaccacg cccgtgtcct tgtaatgggc ttcccaccac
     2041 cattggtggc cgatgaccct gatgtcggcc tggtccttct tggacaggtc cggatagtac
     2101 ttgcccatgc cgtcgatggt gagccagatc agccagatga cgatcatgct gggcaccacg
     2161 gtccagatga tctccaccgt gtggttcccg aaatagggct tgggctcggg ggcgccttcc
     2221 ttgtggcggt acttgatcag cgccccgaag accatggtgc agaccaccac catgatgccc
     2281 gcgcagatcc agagcaccgt gatgaaaaga tgggagatgg cggcgggctg ggcggcgagg
     2341 gggtcgaaga cgggcatcgt tgcaacgtgc ggattgaaga cattggcttg gttcatggca
     2401 atggattcct ggtatggaag attgtcgcct gcgaaggaca cagactattc ccggcgaagg
     2461 gccgattcaa gcggaaatcg cctcttatta aaagcatggc tttcagtcgc ggttgaattt
     2521 gaaataataa atcaaaaagt cgaaagtttc attttgaatc cgaccggacc cggatttttt
     2581 tgggggtcgg acgggccggc ggatcgtgaa accgaggggg ggaagggtgg tcttgaaaat
     2641 caagatcaat tagcccgccg ccggagggtg gggcgggctt gtctcgtgat ttggcttggg
     2701 gaaggcgctt aggggcggtc cagttccttt ttcacgtccg cctccaactc cgcgggcgtg
     2761 acctgggaca cgaaacgcgc caccggacgg ccgtcgcggc cgatgaggaa tttggtgaag
     2821 ttccagccga tgtcctcctt gccctgggga tcgggttcgg cgtgcttgag gaagcggtag
     2881 aggggatggg tgcccgggcc gttcacctcg atcttggcga acagcgggaa gctgac
//
LOCUS       DASTJJ010000814         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1823679, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000814 DASTJJ010000000
VERSION     DASTJJ010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823679"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="VFR02_09800"
     CDS             <1..>1023
                     /locus_tag="VFR02_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9870772.1"
                     /translation="ILGGGPNRIGQGIEFDYCCVHGAFALRQDGFEVIMVNNNPETVS
                     TDYDTSNRLYFEPLTFEDVMNILDRERPKGVIVQFGGQTPLKLAVALEGAGAPIIGTS
                     PHSIDVAEDRKLFGAMLKKLKIRQPENGIAMNVKQAKPVARKVGYPVVVRPSYVLGGR
                     AMEIAYDEESLDEFVAKAAEAAPDKPILIDHFLEDALEVDVDCVCDGKDVLVAGIMEH
                     IEEAGIHSGDSACVIPPYTLREDQIAAIKKNTREMALALKVKGLMNVQYAIKNDMVYV
                     LEVNPRASRTVPFVSKATGTPWAKVAARLMAGKTLAQLKVKETPFLKHIAVKESVFPF
                     NRFPGVD"
BASE COUNT          197 a          337 c          330 g          159 t
ORIGIN      
        1 atcctgggcg gcgggcccaa ccgcatcggc cagggcatcg agttcgacta ttgctgcgta
       61 cacggcgcct tcgcgctgcg ccaggacggc tttgaggtca tcatggtcaa caacaacccg
      121 gagaccgtct ccaccgacta cgacacctcc aaccggctct atttcgagcc cctgaccttc
      181 gaggacgtga tgaacatcct ggaccgggaa aggcccaagg gcgtcatcgt gcagttcggc
      241 ggccagacgc cgctgaagct ggcggtggcc ctggaggggg ccggcgcccc catcatcggc
      301 acctcgcccc attccatcga cgtggcggag gaccgcaagc tcttcggcgc catgctcaag
      361 aagctcaaga tccgccagcc cgagaacggc atcgccatga acgtcaagca ggccaagccc
      421 gtggcccgca aggtgggcta cccggtggtg gtgcgcccct cctacgtgct gggcgggcgg
      481 gccatggaga tcgcctatga cgaggaatcc ctggacgagt tcgtggccaa ggccgccgag
      541 gcggcgcccg acaagcccat cctgatcgac catttcctgg aggacgccct ggaagtggac
      601 gtggactgcg tgtgcgacgg caaggacgtg ctggtggcgg gcatcatgga gcacatcgag
      661 gaagcgggca tccattcggg cgattcggcc tgcgtgatcc cgccctacac cctgcgcgag
      721 gaccagatcg cggccatcaa gaagaacacc cgcgaaatgg ccctggccct gaaggtgaag
      781 gggctcatga acgtgcagta cgccatcaag aacgacatgg tctacgtgtt ggaagtgaac
      841 ccccgggcgt cccggaccgt gcctttcgtg tccaaggcca ccggtacgcc ctgggccaag
      901 gtggcggccc ggctcatggc gggcaagacc ctggcgcagc tgaaggtgaa ggagacgccc
      961 ttcctcaagc acatcgccgt gaaggaatcg gtcttcccct tcaaccgttt cccgggcgtc
     1021 gac
//
LOCUS       DASTJJ010000815         1318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_600632, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000815 DASTJJ010000000
VERSION     DASTJJ010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600632"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..>1318
                     /locus_tag="VFR02_09805"
     CDS             78..>1318
                     /locus_tag="VFR02_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9870773.1"
                     /translation="MPGLKGVLPAARWTARHWAREPWRALVTLAGVALGIALVAAIRL
                     SSEAAQSSFEDSLSLLSGRADLSLTSPSGPFSESLFAQLAPTRAFGLMMPLVTGKMAP
                     ASAPKDQLEVLGPDLLKDNLVRDYHLLGPGGAALGWPDLLKVLPNPDVVFLTQKFADR
                     NGLKAGERADFLVGDKKVQLLVAGLLADEGAGRALDGSLALMDLAAAQALFGKVGKLD
                     GIQWVTRSPADRQKLQTALTAMLPPGFTAERPSARSGQVEKLLRAFRTNLLALALVSL
                     LVGAFLVYNSMSIAVLRRLGEIGTLRALGATRGDILRLVLFEAAALGLLGSTAGIFLG
                     RVMAKGAMDLVGATLTNLYLYEPLGNRLPADGDTWLWVLFGFLLVTLAGLPPALWAAQ
                     VPPAFSTRAGAPETLFGRRAP"
BASE COUNT          171 a          463 c          448 g          236 t
ORIGIN      
        1 ccgggaagcg gccgacctgg gggacacgct catcctgctc aaggacgggg ccgtcaccgg
       61 gctctacccg cgccaacatg cccggcctta agggcgtcct gccggcggcc cgctggacgg
      121 cgcgccactg ggcgagggag ccctggcggg ccctggtgac cctggcgggc gtggcgctgg
      181 gcatcgcctt ggtggcggcc atccggcttt ccagcgaggc cgcgcaaagc tccttcgagg
      241 attcattgtc cctgctttcg ggccgggccg atctgtccct gacctccccc agcgggccct
      301 tttccgaatc cctcttcgcc cagctcgcgc ccacccgcgc cttcggcctg atgatgccgc
      361 tggtcaccgg caagatggcg cccgcatccg cccccaagga ccaactcgag gtgctggggc
      421 cggacctgtt gaaggacaac ctggtgcgcg actaccacct gctgggtccg gggggcgcgg
      481 ccctgggctg gccggacctg ctcaaggttt tacccaaccc cgacgtggtc ttcctcacgc
      541 aaaaattcgc cgaccgcaac ggcctgaagg ccggcgaaag ggccgacttt ttggtggggg
      601 ataaaaaggt ccaactcttg gtggcgggcc tcctggccga cgagggcgcg ggccgggcgc
      661 tcgacggatc cctggccctg atggacctgg cggcggccca ggcccttttc ggcaaggtgg
      721 gcaagttgga cgggatccaa tgggtgaccc gttccccggc cgaccggcaa aagctccaaa
      781 ccgcgcttac cgccatgctt ccgccggggt tcacggccga gcgcccttcc gcgcgcagcg
      841 gccaggtcga gaagctgctg cgcgccttcc gcacgaacct gctggccctg gcgctggtgt
      901 ccctcttggt cggggccttt ttggtctaca actccatgtc catcgcggtg ctccggcggt
      961 tgggtgagat cggcacttta agggcgctgg gcgccacccg gggcgacatc ctccggttgg
     1021 tgctctttga ggcggcggcc ttagggctat tggggtcgac cgcgggtatc tttttgggca
     1081 gggtcatggc caagggcgcc atggacctgg tgggcgccac cctcaccaac ctttatctat
     1141 atgagccgct gggcaaccgc cttcccgccg acggcgacac ctggctttgg gtgctgttcg
     1201 gattcctctt ggtgacgctg gcggggcttc ccccggccct ctgggccgcc caggtgccgc
     1261 ccgccttttc cacccgggcg ggggcgcctg agaccctctt cgggcgcagg gcgccgca
//
LOCUS       DASTJJ010000816         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1336267, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000816 DASTJJ010000000
VERSION     DASTJJ010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336267"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1490
                     /locus_tag="VFR02_09810"
     CDS             30..1490
                     /locus_tag="VFR02_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9870774.1"
                     /translation="MNFENYQLENFYDELFDRSGNPRAAAAPLIARLKSLPPGDLKRQ
                     QKAAEAELMQLGITFTVYGSQEGTEKIFPFDIIPRVIEAAEWKQIEAGLSQRIQALNL
                     FIDDIYHGQKILKDKVIPADMVKTSSGFRYQCLGLNPPRRIWIHITGTDLVRDDSGKF
                     FVLEDNLRCPSGVSYVLENRSVLKRTLPHVFQSMPVRPVDEYSHRLLDTLEYLAPPTV
                     RHPRVVVLTPGPYNSAYFEHAFLAQQMGAELVEGRDLSVIDGNVVMRTTKGYQKVDVI
                     YRRVDDDFLDPKVFRPDSTLGVPGLMDVYRAGRVAIANAPGTGIADDKAIYTYVPQII
                     RYYLGQDAILPNVPTFVCWDEAQCQHVLANLDKLVVKAVNQSGGYGMLVGPHASKAER
                     EKFAQLIRENPRDYIAQPTLSLSRVPVIVDDHLEGRHVDLRPYVLYGLDLYVMPGGLT
                     RVALKKGSLVVNSSQGGGSKDTWVLADTSPASSKGA"
     gene            1494..2477
                     /locus_tag="VFR02_09815"
     CDS             1494..2477
                     /locus_tag="VFR02_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET9870775.1"
                     /translation="MLSRLADSTYWMSRYIERAENVARFIDVNLYLMLDQTPLAERQQ
                     WLPLVGTTGDHADFQARYKEATRENVLQFLIFDSQNPNSILSCLQKARENARTMREVI
                     SSEMWTQVNGFYYMVQNAMSSQTTKDFPQTFCREVKQASHLFQGVTDATFTHGEAWHF
                     IQLGRLFERADKTARILDVKYFILLPKVEDIGTPIDLILWTALLKSATAYEMYRKKHN
                     RIDPFRIAEYLMLDREFPRSMRHCLIKAEESLRAITGTPLGTYANAAERRLGRLRSEL
                     DYGNIQEIIRSGLHEYLDDFETKLNALGTDLFAIYLAASPVPGYFTVKGDQ"
     gene            2551..>2760
                     /locus_tag="VFR02_09820"
     CDS             2551..>2760
                     /locus_tag="VFR02_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET9870776.1"
                     /translation="MTFCVAMKVKDGLVALADTRITSGTEQITGRKVSIHQNGHHSLF
                     LMTSGLRSARDKALTYFNEVLEAQPQ"
BASE COUNT          539 a          950 c          810 g          461 t
ORIGIN      
        1 gttcaacctt atggaccagg aaccacacca tgaacttcga aaactaccaa ttggaaaact
       61 tctatgacga gctgttcgac cgttcgggca atccccgggc cgccgccgcc cccctgatcg
      121 cccggctcaa atccctgccc cccggcgacc tgaagcggca acaaaaggcc gccgaggccg
      181 aattgatgca gttgggcatc accttcacgg tctacggcag ccaggaaggg accgaaaaga
      241 tcttcccttt cgacatcatc ccccgggtca tcgaggccgc cgagtggaag cagatcgagg
      301 ccgggctgtc ccagcggatc caggccttga accttttcat cgacgacatc taccacggcc
      361 aaaagatcct gaaggacaag gtcatccccg cggacatggt caagacctcc tcggggttcc
      421 gctaccaatg cctggggctc aacccgccgc gccgtatttg gatccacatc acgggcacgg
      481 acctggtgcg ggacgacagc ggcaagttct tcgtcctgga ggacaacctg cgctgcccct
      541 cgggggtgtc ctacgtcctg gagaaccgct ccgtcctgaa gcgcaccctg ccccacgtct
      601 tccaatccat gcccgtgcgc ccggtggatg aatattccca ccgcctgctg gacaccctgg
      661 agtatttggc gccgcccacc gtccggcatc cccgggtggt ggtgctcacc cccggcccct
      721 acaactcggc ctacttcgag cacgccttcc tggcccaaca gatgggcgcc gagttggtgg
      781 aaggccggga cctgtcggtc atcgacggca acgtggtcat gcgcaccacc aagggctacc
      841 agaaggtgga tgtcatctac cgccgggtgg acgacgattt cctcgacccc aaggtcttcc
      901 ggcccgattc caccctgggc gtgcccggcc tgatggacgt gtaccgggcg ggccgggtgg
      961 ccatcgccaa cgcgccgggc acgggcatcg cggacgacaa ggccatctac acttatgtcc
     1021 cgcagatcat ccgctattac ctgggccagg acgccatcct gcccaacgtg ccgaccttcg
     1081 tgtgctggga tgaggcccag tgccagcatg tgttggccaa cctggacaaa ctggtggtga
     1141 aggcggtcaa ccagtccggc ggctacggca tgctggtggg cccccacgcc tccaaggccg
     1201 aacgggaaaa gttcgcccaa ctgatccgcg agaacccgcg ggactacatc gcccaaccca
     1261 ccctgtccct ctcccgggtg ccggtcatcg tggacgacca tttggaagga cggcacgtgg
     1321 acctgcggcc ctacgtgctt tacggcctgg acctctacgt catgcccggg ggcctgaccc
     1381 gggtggcgct gaaaaagggg tcgctggtgg tgaattcctc ccagggcggc ggcagcaagg
     1441 acacctgggt gctggccgac acctcccccg cctcgtccaa gggggcctga cccatgctca
     1501 gccgcctggc cgattccacc tactggatga gccgctatat cgaacgggcc gagaacgtgg
     1561 cccgcttcat cgacgtgaac ctgtatctga tgctggacca gacccccctg gccgaacggc
     1621 agcagtggct gcccctggtg ggcaccacgg gcgaccacgc cgatttccag gcccgttata
     1681 aggaagccac ccgggagaac gtgctgcagt tcctcatctt cgactcccag aaccccaact
     1741 cgatcctttc ctgcctgcaa aaggcccggg agaacgcccg gaccatgcgg gaggtcattt
     1801 cgtccgaaat gtggacccag gtgaacggct tctactacat ggtgcagaac gccatgagtt
     1861 cccagaccac caaggatttc ccccagacct tctgccgcga ggtcaagcag gccagccacc
     1921 tgttccaggg cgtgaccgac gccaccttca cccacggcga ggcctggcat ttcatccagc
     1981 tggggcgcct cttcgagcgg gccgacaaga ccgcccgcat cctggacgtg aagtatttca
     2041 tcctgctgcc caaggtggag gatatcggca cccccatcga cctcatcctc tggacggcgc
     2101 tcttgaagtc ggccacggcc tacgagatgt accgcaaaaa gcacaaccgc atcgacccct
     2161 tccgcatcgc cgagtacctg atgctggacc gggaattccc ccggtccatg cggcactgcc
     2221 tgatcaaggc cgaggagtcc ctgcgggcca tcacgggcac gcccctgggc acctacgcca
     2281 acgccgccga acgccggctg gggcggctgc ggtccgagtt ggactacggc aacatccagg
     2341 agatcatccg ctcgggcctg cacgagtacc tggacgactt cgagacaaag ttgaacgccc
     2401 tgggcacgga cctgttcgcc atctacctgg cggccagccc cgtgcccggt tatttcacgg
     2461 tcaagggcga ccagtagcgg acgccggaaa acaccgcggc gccgcggagg agggttgggt
     2521 ctctatcttg gaacagcaaa ggaaccggca atgacatttt gcgtggccat gaaggtgaag
     2581 gacggcttgg tggccctggc ggacacccgc atcaccagcg ggaccgagca gatcacggga
     2641 aggaaggtct ccatccacca gaacgggcac cattcgctct tcctgatgac ctcgggcctg
     2701 cggtcggccc gggacaaggc cttgacctac ttcaacgagg tgttggaagc ccagccccaa
//
LOCUS       DASTJJ010000817         1459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_845854, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000817 DASTJJ010000000
VERSION     DASTJJ010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845854"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFR02_09825"
     CDS             <1..281
                     /locus_tag="VFR02_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870777.1"
                     /translation="WHDAVLAVFKRRQTGFLTTDSLLKEMHFRKAIQPKEDKERLEGV
                     LLQMQEAGLIAFNQFKLASLTDQGKAAVEAMTPERLAEIEKADRERAQ"
     gene            278..847
                     /locus_tag="VFR02_09830"
     CDS             278..847
                     /locus_tag="VFR02_09830"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET9870778.1"
                     /translation="MKSPAKPRCPWPVDDPLMVDYHDREWGTPVKDDRKIFEFLVLES
                     FQAGLSWRTILYKRENFRKAFRGFDYRKVAQFGAKDRARLMKDAGIVRNRLKIQAAIG
                     NAQRFLEVQKEFGSFSKYMWGWVKGKPIRNRFKSFKECPAVTPLAEAWAKDLKDRGFR
                     FLGPTVVYAHMQAAGMVNDHLVSCFRHKK"
     gene            1013..1207
                     /locus_tag="VFR02_09835"
     CDS             1013..1207
                     /locus_tag="VFR02_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870779.1"
                     /translation="MPSCLGCGGEMKQVSRKIIKGEQASGHANYKAVYQCGNEKCTEK
                     GAKKSFLDDGMMLIPLPDGK"
     gene            1274..>1459
                     /locus_tag="VFR02_09840"
     CDS             1274..>1459
                     /locus_tag="VFR02_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870780.1"
                     /translation="MTNSTINGKPAPEVMDDSKYAKGTGLFSVYEGVDLPADPAKRCS
                     ALMELGTETLLANHFNIL"
BASE COUNT          349 a          410 c          418 g          282 t
ORIGIN      
        1 cctggcacga cgccgtgctg gccgttttca agcgccgaca aaccggcttc ctcaccaccg
       61 acagcctttt gaaagaaatg catttccgca aggcgatcca gcccaaggag gacaaggaac
      121 ggctggaggg cgtcctgctc caaatgcagg aggcggggct catcgccttc aaccagttca
      181 agctggcttc cctcaccgac cagggcaagg ccgcggtgga ggccatgacg cccgaaaggc
      241 tggccgagat cgagaaggcc gaccgggaga gggcccaatg aaaagccccg ccaagccccg
      301 ctgcccctgg cccgtggacg acccgctcat ggtcgactac cacgaccggg aatggggcac
      361 cccggtcaag gacgaccgga agatcttcga gttcctggtg ctggagtcct tccaggccgg
      421 gctttcctgg cgcacgatcc tttacaagcg cgagaatttc cggaaggcct tccggggctt
      481 cgattaccga aaggtggccc aattcggcgc caaggaccgg gcgcgcctca tgaaggacgc
      541 cggcatcgtc cgcaaccgcc tgaagatcca ggccgccatc ggaaacgccc agcggttcct
      601 ggaggtccaa aaggaattcg gatccttttc caagtacatg tggggttggg tgaagggaaa
      661 acccatccgc aaccggttca aaagcttcaa ggaatgcccg gcggtgacgc ctctggcaga
      721 agcctgggcc aaggacctga aggaccgggg tttccgcttc ctggggccca cggtggtcta
      781 cgcgcacatg caggcggcgg gcatggtgaa cgaccatttg gtttcctgct ttaggcataa
      841 aaaatgaatt aattttcacc acgaaggcac caaggaaggc ctcaccctta ttgttaggaa
      901 attaaaaagc ttcttttggc agttacgaat ttgaggcttg tcatttttaa cttggtgcct
      961 tggtgtcttg gtggtgaaag ggatcattaa agtctttaaa aatgggggtt ttatgccttc
     1021 ttgtttgggt tgcggcgggg aaatgaagca ggtgtcgcgc aagatcatca agggcgaaca
     1081 ggcctcgggc cacgccaact acaaggccgt ctaccaatgc ggcaacgaaa aatgcaccga
     1141 aaagggagcg aagaaaagtt ttttggacga cggcatgatg ctcattccac tgccggacgg
     1201 gaagtaaaac caaagtaaac cgtcagtttt tattaacggt ttttttattt tttaccttaa
     1261 aataaaaccc gatatgacca acagcacgat caacgggaaa ccggcgccgg aagtcatgga
     1321 cgattccaag tacgccaagg gaaccggcct cttctcggtc tatgagggcg tggacctgcc
     1381 cgccgacccg gccaaacgct gctccgccct gatggaactg ggcaccgaga ccctgctggc
     1441 caaccacttc aacatcctc
//
LOCUS       DASTJJ010000818         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1581640, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000818 DASTJJ010000000
VERSION     DASTJJ010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581640"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFR02_09845"
     CDS             <1..1005
                     /locus_tag="VFR02_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET9870781.1"
                     /translation="AGVGTIGLVDFDTVDASNLQRQILFTTEDVGKPKVEVAAKRLKA
                     LNPDVTVKTYAVALKSDNIMDILKDYDMIIDGTDNFPTRYLTNDAAVLSGKPNVYGSI
                     FRFEGQVTVFKTPEGPCYRCLYPEPPPPGEVPSCAEGGVMGAMVGTVGAIQATEAIKL
                     ITGAGKPAVGKFVIYNSLDMNFRTLKLHKDPECPVCGKNPTVKQLIDYEQFCGMKVED
                     TQTKEVAAAKDEITALELKVMMDEKRDFVLLDVREPNEWDIAHIEGAQLLPLSQLPQR
                     FHELDKYKGKDIAVHCKSGVRSRKAINFLKDQGFSRLTNVAGGILGWSDQVDSSVPKY
                     "
     gene            1010..1642
                     /locus_tag="VFR02_09850"
     CDS             1010..1642
                     /locus_tag="VFR02_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870782.1"
                     /translation="MDPEASSGAPEARTLGVARVAFAERFSAYVDYRTEEADPLSRER
                     DAIQLFILTLVKLLLLLGGNKQAPMLLEYLQKAMGDTVTPKGLQRPLILGKGQELLAE
                     PPEAPKKDITLALKQEEEARLAVLEIPPGEEGLYYPVTAVFFLQYLLKTLSEPSLFYL
                     MLVMGGLMEYYEKIGKTTDLAALTAGPAYAFSTASRYIEEERRRSAGPEA"
     gene            complement(1639..2121)
                     /locus_tag="VFR02_09855"
     CDS             complement(1639..2121)
                     /locus_tag="VFR02_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET9870783.1"
                     /translation="MPFLRFVHLFTFIFWYGALLYFTFIQAPLLFKTLPRDLFGLVQS
                     KLFPAYYVISYVCGTLLVVTYHLLHPLKSYVPQDCVKITALCLMLLFSLGQGLWIGPK
                     VGKLMVERQAAEQAKDQPQVEALTKRFGMAHGVSSLFNLVVILAGTVYLVYLFKEWRP
                     "
     gene            2098..2403
                     /locus_tag="VFR02_09860"
     CDS             2098..2403
                     /locus_tag="VFR02_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870784.1"
                     /translation="MHKTQKGHGGPFKMGWPILKRDYGFYKASKRELRTSNKNLFRRQ
                     DAKGAKNCGEKTPENRINSKAFLGELGVCPNIYLLVMGPPLGVAVEMAFILIFVSSS"
     gene            2728..3072
                     /locus_tag="VFR02_09865"
     CDS             2728..3072
                     /locus_tag="VFR02_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9870785.1"
                     /translation="MDPTPLQIRFPKTGQACEADFGDSLVDAVFRHDLPVRFKCERAV
                     CTTCLVEVLKGMENLAPPGARELQTLESAGLSGRFRLACQVELRGDVELDYVPLLDPR
                     RKPRPTGDDLLV"
     gene            3138..>3748
                     /locus_tag="VFR02_09870"
     CDS             3138..>3748
                     /locus_tag="VFR02_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9870786.1"
                     /translation="MPPLVEVRNLSKTYGPPGAGGVEALRKVGFKVEAGEFISLMGPS
                     GCGKSTLLQLLGGLDTPSEGEVLFEGRSLGSLPDDERSLLRRKSFGFVFQFFNLLPNF
                     NALENVELPLLLAGGDQARARGRAQALLERVGLSQRGNHRPAELSGGEQQRVALARAL
                     VHGPRLLLADEPTGNLDSVTGGSVLKLITQVAREMDCAVVMAT"
BASE COUNT          752 a         1184 c         1173 g          639 t
ORIGIN      
        1 gcgggcgtgg gcaccatcgg cctggtggac ttcgacacgg tggacgcctc gaacctgcag
       61 cgccagatcc tcttcaccac cgaggacgtg ggcaaaccca aggtcgaggt ggccgccaag
      121 cgcctcaagg ccctcaaccc cgacgtgacg gtcaagacct acgcggtggc cctcaagagc
      181 gacaacatca tggacatcct gaaggattac gacatgatca tcgacgggac ggacaatttc
      241 ccgacccgtt acctcaccaa cgacgcggcc gtgctctcgg gcaagcccaa cgtgtacggc
      301 tccatcttcc gtttcgaggg ccaggtgacg gtcttcaaga cgcccgaggg cccctgctac
      361 cgctgcctct accccgagcc gccgcccccg ggcgaggtgc cgtcctgcgc cgagggcggc
      421 gtcatgggcg ccatggtggg cacggtgggc gccatccagg ccaccgaggc catcaagctc
      481 atcacgggcg cgggcaagcc ggcggtcggc aagttcgtca tctacaactc gctggacatg
      541 aacttccgca ccctcaagct gcacaaggac cccgaatgcc cggtctgcgg caagaacccc
      601 acggtcaagc agctcatcga ctacgagcag ttctgcggca tgaaggtgga ggacacccag
      661 accaaggaag tggccgccgc caaggacgag atcaccgcgc tcgagctcaa ggtcatgatg
      721 gacgagaaac gggatttcgt gctcttggac gtgcgcgagc ccaacgagtg ggacatcgcc
      781 cacatcgagg gggcgcagct gctgccgctg tcccaactgc cccagcgctt ccacgagttg
      841 gacaagtaca agggcaagga catcgcggtg cactgcaaga gcggcgtgcg cagccgcaag
      901 gccatcaact tcctcaagga ccagggtttc agccggctca ccaacgtggc ggggggcatc
      961 ctgggttgga gcgaccaggt ggactcctcc gtccccaagt actgatccct tggacccgga
     1021 ggcttcgtcg ggcgcgcccg aggcccgcac gctcggtgtg gcccgggtgg ccttcgccga
     1081 gcgcttctcg gcctacgtcg actaccgcac ggaagaggcc gaccccctgt cccgcgaacg
     1141 ggacgccatc cagcttttca tcctgacgtt ggtcaaactc ctcctgcttt taggcgggaa
     1201 caagcaggcg cccatgctgc tggagtacct ccaaaaggcc atgggcgaca cggtgacccc
     1261 gaagggcctg caacgccccc tcatcctggg gaagggccag gaacttttgg ccgagccgcc
     1321 cgaggcgccc aagaaggaca tcacgctggc cttgaagcag gaagaggaag cgaggctggc
     1381 ggtgttggag atcccgcccg gcgaggaagg cctctattac ccggtcacgg ccgtcttttt
     1441 cctgcagtac ctcttgaaga ccctgtccga gccctcgctt ttttacctca tgctggtcat
     1501 gggggggctg atggaatatt acgagaagat cggcaagacg accgacctgg cggccctcac
     1561 cgcgggcccg gcctacgcct tttccacggc ctcgcgctac atcgaggaag aacgcagacg
     1621 gtccgccgga cccgaagcct aaggccgcca ttccttgaac aaatagacca ggtagaccgt
     1681 cccggccagg atgacgacca ggttgaagag gctggagacg ccgtgggcca tgccgaagcg
     1741 cttggtcagg gcctccacct ggggttggtc cttcgcctgc tcggcggcct gccgctccac
     1801 catcaatttc cccaccttgg ggccgatcca caggccctgg cccagcgaaa agagcagcat
     1861 caggcacagc gcggtgatct tcacgcagtc ctggggcacg taactcttca gcgggtgcag
     1921 caggtggtag gtgaccacca agagggtgcc gcagacgtag ctgatgacgt aataggccgg
     1981 gaaaagcttg ctctgcacca ggccgaagag gtcccggggc agggtcttga agagcaaggg
     2041 ggcctggatg aaggtgaagt agagcagggc gccgtaccag aagatgaagg tgaagaggtg
     2101 cacaaaacgc aaaaagggca tggggggcct ttcaaaatgg gatggcccat tttaaaacgg
     2161 gactatggct tttacaaagc aagcaaaaga gaacttagaa cgtctaacaa aaacctgttt
     2221 cgccgccaag acgccaaggg cgccaagaac tgcggagaaa aaacgccgga aaaccggata
     2281 aattcgaagg cctttcttgg cgaacttggc gtctgcccca acatttattt attggtaatg
     2341 gggccgccct tgggcgtggc ggtagaaatg gcctttattt tgatttttgt tagcagctct
     2401 taaaacagtg ataaagaccg atgaaatctg atgtttgaaa ataagaggcc gtcacaaaac
     2461 caaaggccat gagcatggat ggacacccat caacgcagcc taggaagcga tttccatcgg
     2521 ttctttacag cgttcatcgg cgttcatcat tgctgattga attttggtag agtcgctaaa
     2581 gatcaggctt tatcagcttt tatcaatgtt ccaattgtct ttgcgttgcg aagcgcatga
     2641 ttccgtttaa aaaccccccg gggggaatag caaccggccc cgcgcattgc gccccccggc
     2701 ccgccttcct ataatgcgcc ccaagccatg gaccccaccc ccctccagat ccgtttcccc
     2761 aagaccggcc aggcctgtga agccgacttc ggcgacagcc tggtggacgc cgtcttccgc
     2821 cacgacctgc ccgtgcggtt caagtgcgag cgggcggtct gcaccacctg cctggtggag
     2881 gtcttgaagg gcatggagaa cctcgcgccc ccgggggcgc gggaactgca gaccctggag
     2941 tcggcgggcc tttcgggccg tttccgcctg gcctgccagg tggagctgcg cggggacgtg
     3001 gagctggact acgtgccgct cttggacccc cgccgcaaac cccggccgac cggggatgac
     3061 ctgttggtct aggaacacct gttcttgcag gccgcttggt ggtgaaatag gccggatcca
     3121 ttcgaacaag gaagcccatg ccccctctgg tggaagtcag gaacctttcc aagacctacg
     3181 gcccgcccgg ggccgggggc gtggaggcgc tgcgcaaggt cggcttcaag gtggaagcgg
     3241 gcgagttcat ttcccttatg ggcccctcgg gctgcggcaa gtcgaccctg ctgcagcttt
     3301 tgggcggcct ggacaccccc agcgagggcg aggtgctctt cgagggccgg tccctcgggt
     3361 ccctcccgga cgacgagcgc agcctcttgc ggcgcaaaag cttcgggttc gtcttccagt
     3421 tcttcaacct gctgcccaac ttcaacgccc tggagaacgt ggagctgccg ctcttgctcg
     3481 ccggggggga ccaggcccgc gcccggggac gcgcccaggc gctgctggag cgggtggggc
     3541 tttcccaaag gggcaaccac cggcccgccg agctctccgg cggcgaacag cagcgcgtgg
     3601 ccctggcccg ggccctggtg cacgggcccc ggctgctcct ggccgatgaa cccacgggga
     3661 acctggacag cgtgaccggg gggagcgtct tgaagctcat cacccaggtg gcccgcgaga
     3721 tggactgcgc ggtcgtcatg gccaccca
//
LOCUS       DASTJJ010000819         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1824515, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000819 DASTJJ010000000
VERSION     DASTJJ010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824515"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="rho"
                     /locus_tag="VFR02_09875"
     CDS             complement(<1..929)
                     /gene="rho"
                     /locus_tag="VFR02_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9870787.1"
                     /translation="MDIADLKKKTVAELTEVARELNLEEVGSLKKQDLIFKILETQTQ
                     RNGVINAEGVLEILQDGFGFLRSANYNYLPGPDDIYVSPSQIRRFNLRTGDTVTGQVR
                     PPKEGERYFALLKVDTINFEPPDAVKHKVLFDNLTPIYPNHRIWLETSPDDLSMRVMD
                     LLIPLGKGQRGLIVAPPRTGKTILLQKIANSIAKNHPEIYLIVLLIDERPEEVTDMQR
                     SVKGEVVSSTFDEPPERHVTVAEIIIEKAKRLVEHKKDVVILLDSITRLGRAYNTIVP
                     SSGKVLSGGVDSNALQRPKRFFGAARDIEEGGSL"
     gene            complement(1279..2700)
                     /locus_tag="VFR02_09880"
     CDS             complement(1279..2700)
                     /locus_tag="VFR02_09880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9870788.1"
                     /translation="MRDRSQRIGGALLGALVAVCGFIPLAGPYLLRLPVFFMLPLAYL
                     APEAAAWCLGAYSVGIVAEKALGLSELSSVWIWPEFLATAALAAAAVRLRRSQERLRG
                     QSEERLQARREEFAGFFRNSEGMKRENAQLEKQLRNIEHLYDVIKEAGTTLSVQEMLE
                     VAREFTERMFDLPHFLIGVLASEGKRFEIRVASGCDEVFLRSFEVDLDSGGLGAAFVR
                     DKKVQWIPDFAADPRLARVRQLPVRSLAFFPFVVQDRVIGFLCTFVSAGASLDQETLA
                     NLEVFSNQIAIGLQKSLLYEKVQKLSITDGLTKLYSHRYFKQRLEAELILANRYSSQL
                     SLLILDIDHFKHYNDTYGHVAGDSVLTEVAKILKSQSDVTHVVARYGGEEMVLLAPET
                     TKDQALELAERIRRAIETHRFAVGKESTEVTVSVGLATFPQDARTNLDLISKADKALY
                     AAKGSGRNRVVAYPLAEGGRGAN"
     gene            complement(2690..>3586)
                     /locus_tag="VFR02_09885"
     CDS             complement(2690..>3586)
                     /locus_tag="VFR02_09885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9870789.1"
                     /translation="LLLFDSSIGKAVHVVRYSVDSDMEVRLNRERLNRSEPFNAWVIA
                     QAKSLFVNDASSDFRFQNFAAESKIKSLVSAPFLAGNEMLGLVRMESAVPGVFRQDDA
                     RLLSTFADLGTVALEHVALYKQTIELAITDGLTGLYVQRYYKERVRDEVFRALEHGFP
                     LCVLMIDVDHFKSYNDRYGHLVGDKVLKVVSQILKETIRTVDLAARYGGEEFSVLLLK
                     TPLDGAEKVAERIREKVKNQEVLAAKEVTHVTVSIGVAELQPSWKNVETFIDAADQAL
                     YQAKAAGRNRVRVARPGQTHAG"
BASE COUNT          621 a         1148 c         1148 g          669 t
ORIGIN      
        1 aggctgccgc cctcctcgat gtcgcgggcc gcgccgaaga agcgcttggg ccgttgcagg
       61 gcgttggaat ccacgccccc cgagagcacc ttgcccgagg acggcacgat ggtgttgtag
      121 gcccggccca ggcgggtgat cgaatccagc aggatgacca cgtccttctt gtgctccacc
      181 aggcgcttgg ccttctcgat gatgatctcg gccaccgtca cgtgccgctc aggcggctcg
      241 tcgaaggtgg aggacaccac ttcgcccttc accgagcgct gcatgtcggt gacctcctcc
      301 ggccgttcgt cgatcaacaa cacgatcagg tagatctcgg ggtggttctt ggcgatgctg
      361 ttggcgatct tctgcagcag gatggtcttg ccggtccggg gcggggccac gatcaggccg
      421 cgctgcccct tgcccaaggg gatcaagagg tccatcaccc gcatggacag gtcgtccggc
      481 gaggtctcca accagatgcg gtggttgggg tagatggggg tcaggttgtc gaagagcacc
      541 ttgtgcttga ccgcgtccgg cggctcgaag ttgatggtgt cgaccttcag cagggcgaaa
      601 tagcgctccc cttccttggg cgggcgcacc tggccggtga cggtgtcccc cgtgcgaagg
      661 ttgaaacggc ggatctggga gggcgagacg tagatgtcgt ccgggccggg caggtagttg
      721 tagttggccg accgcaggaa gccgaagccg tcctgcagga tctccaacac gccctcggcg
      781 ttgatgacgc cgttgcgctg ggtctgggtc tccaggatct tgaagatcag gtcctgtttc
      841 ttgagcgacc ccacctcctc cagattgagc tcccgggcca cctccgtcaa ctcggcgacg
      901 gtcttctttt tcagatccgc gatatccata aacgcttcgg tcctttcccg gattcccgcg
      961 gggacacccg tgggaaggct ggatgaaatt gtgaggatga tggtttcaac gggcttttcg
     1021 ggcgtcccgg accccgttgg gtgaggctcg gcttttcttc ccgggctggg cccggggctt
     1081 ccgcgcgcaa ctggggcgcg gaccagacta gtgctttgat tcagtggcct ttgggttggt
     1141 gagggataag attgggcgtt ttggggattc ttccatcgaa gatctatcgg aagtctattg
     1201 ggaatccaga ggatcggctc aagacggctt gacggcagcg aaatcatggt accggcggcg
     1261 gcggcggcct gtcaaccatt aattcgcccc ccggccgcct tccgccaggg gataggccac
     1321 cacgcggttg cgcccgctcc ccttggccgc gtagagggcc ttgtcggcct tgctgatcag
     1381 gtccaggttg gtgcgggcat cctgggggaa ggtggccaag cccaccgaaa cggtcacttc
     1441 cgtggactcc ttgcccacgg cgaaacggtg ggtctcgatg gcccgccgga tccgctcggc
     1501 caattccagg gcctggtcct tggtggtctc gggggccaac aacaccatct cttccccgcc
     1561 gtaacgggcc accacgtggg tcacgtcgga ttggctcttg aggatcttgg ccacctcggt
     1621 caggaccgaa tcgcccgcca cgtggccgta ggtgtcgttg taatgcttga aatggtcgat
     1681 gtccaggatg agcaggctca gttgggacga atagcggttg gccaggatca attcggcctc
     1741 caggcgctgc ttgaagtacc ggtgggaata aagcttggtc aggccgtccg tgatggacag
     1801 tttctgcacc ttctcgtaca gcagggactt ctgcagcccg atggcgatct ggttggaaaa
     1861 gacctccaga ttggccaggg tctcctggtc cagggaggcg ccggcggaca cgaaggtgca
     1921 gaggaacccg atcacccggt cctgcaccac gaaggggaaa aaggccaggg accggaccgg
     1981 cagctgccgc acccgcgcca accgcgggtc ggcggcgaaa tcggggatcc attggacctt
     2041 cttgtcgcgg acgaaggccg cgcccaaccc gcccgaatcc aggtccacct cgaaggaccg
     2101 caggaagacc tcatcgcaac ccgaagccac ccggatctcg aaacgcttgc cctcgctggc
     2161 cagcaccccg atcaggaaat ggggcaggtc gaacatgcgc tcggtgaatt cccgggccac
     2221 ttccagcatt tcctgcacgc tcaaggtcgt gcccgcttcc ttgatgacgt cgtacaggtg
     2281 ctcgatgttg cgcaattgct tttccagctg ggcgttctcc cgtttcatgc cctccgaatt
     2341 gcggaagaac cccgcgaact cctcccggcg ggcctggagc ctctcctcgg actgcccccg
     2401 caggcgctcc tgggaacgcc gcagcctcac cgcggcggcc gccaaggcgg ccgtggccag
     2461 gaactcgggc cagatccaga ccgaggacag ttcggaaagg cccaaagcct tctccgccac
     2521 gatgcccacc gaataggcgc ccaggcacca ggcggccgcc tcgggggcca aataggcgag
     2581 gggcaacatg aaaaagaccg gcaggcgcaa aagatagggg cccgccagcg ggatgaaccc
     2641 gcagaccgcc accagggccc ccaagagggc tccgccgatc cgctgggacc tatcccgcat
     2701 gggtctgtcc cggccgggcc acccgcacgc ggttgcgtcc cgcggccttg gcctggtaaa
     2761 gcgcttggtc ggcggcgtcg atgaaggtct ccacgttctt ccaggaaggc tgcagttcgg
     2821 ccaccccgat ggacacggtc acgtgggtga cctccttggc ggccagcacc tcctggttct
     2881 tcaccttctc gcggatgcgc tccgccacct tttcggcccc gtccagtggg gtcttcagca
     2941 gcaacacgga aaattcctcg cccccatagc gggccgccag gtccaccgtg cggatggtct
     3001 ccttgaggat ctgggacacc actttcaata ccttgtcccc caccaggtgg ccgtagcggt
     3061 cgttgtagga cttgaagtgg tccacgtcga tcatgagcac gcagaggggg aacccgtgtt
     3121 ccagggcccg gaacacctca tcccgcaccc gttccttgta gtagcgttgc acgtaaagcc
     3181 ccgtcaggcc gtccgtgatg gccagctcga tggtctgctt atagagggcc acgtgttcca
     3241 gggccaccgt gcccaggtcg gcgaaggtgg acaggagccg ggcgtcgtcc tggcggaaga
     3301 cccccggcac ggcgctctcc atgcgcacca gccccaacat ctcgttgccg gccaggaaag
     3361 gcgccgacac caggcttttg atcttggact cggcggcgaa gttctggaac ctgaaatcgc
     3421 tggaagcgtc gttgacgaac aggctcttgg cctgggcaat gacccaggcg ttgaagggct
     3481 cggaccggtt caaccgctcc cggttcaacc gcacctccat gtcgctgtcc acgctgtagc
     3541 gcaccacgtg cacggccttg ccgatgctgc tgtcgaacaa gagcag
//
LOCUS       DASTJJ010000820         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_601455, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000820 DASTJJ010000000
VERSION     DASTJJ010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601455"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFR02_09890"
     CDS             complement(<1..294)
                     /locus_tag="VFR02_09890"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HET9870790.1"
                     /translation="MKRRKKETREGLVEIGDLIAGALKALGAKSDYELFQVGKHCREI
                     LGEAASRALVDVGYEKGRVELKFNHSIWLQEMNFRKAELLGRLQQDLPGLGIQS"
     gene            complement(291..1406)
                     /gene="recF"
                     /locus_tag="VFR02_09895"
     CDS             complement(291..1406)
                     /gene="recF"
                     /locus_tag="VFR02_09895"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET9870791.1"
                     /translation="MHVGSIRLSQFRNYGSLEVPFHPRLNLILGMNAQGKTSLLEALY
                     CLSAARSFRAGGDDEMIQFGRESAAVEGLLQKEKGQDRVFLEFRKHKGKTLTLNGKKQ
                     KKLSALLGQMPAVVFSPDDLFLVKGGPALRRKYLDLTILQLDGGFLPHYQQYERALKQ
                     RNALLRQRPADLDPQLRLWDEPLAEHGAAILARRLKAARELSRWAAQALADLTGGQEK
                     FELRYESALAPGGEGAGKDDIRKAFAKARREEMARGITVVGPHRDDIGLYVNGELLRK
                     FGSQGQQRTAALALKLAQLSLLAEGCHTEPLVLFDDVMSELDEKRQAFFLERLQRGGQ
                     AFLTGTAARDFAAAARSARIFTVEGGRVGLTQEGPAA"
BASE COUNT          307 a          549 c          479 g          333 t
ORIGIN      
        1 gctctggatg cccaaaccgg gcaggtcctg ttggagcctg cccagcaatt ccgccttgcg
       61 gaagttcatc tcctggagcc agatggaatg gttgaacttc agttccaccc ggcccttctc
      121 gtatcccacg tccaccaagg cgcgggaagc ggcctcgccc aggatctccc ggcaatgctt
      181 ccccacctgg aacaactcgt aatccgactt ggcgcccagg gccttcaacg cgcccgcgat
      241 caggtcgccg atctccacca acccttcccg ggtctctttc ttgcgccgct tcacgccgcc
      301 ggcccttcct gggtcaggcc cacccggccc ccttccaccg tgaagatccg ggccgaacgg
      361 gcggcggcgg cgaaatcccg ggccgcggtg ccggtcagga aggcttggcc cccccgttgc
      421 agcctttcca ggaagaacgc ctgccttttc tcgtccaatt cgctcatcac gtcgtcgaac
      481 aacaccaggg gttcggtgtg gcacccctcg gccaggagcg aaagctgggc cagtttgagc
      541 gccagggcgg ccgtgcgttg ttggccctgg ctcccgaact tgcgcagcaa ttccccgttc
      601 acgtagagcc cgatatcgtc ccgatggggg cccaccaccg tgatgcccct cgccatttct
      661 tcccgccggg ccttggcgaa ggcctttcgg atgtcgtcct tccccgcgcc ttcccccccg
      721 ggcgccaggg cgctttcata acgcaactcg aacttttcct ggcccccggt caggtcggcc
      781 agggcttggg ccgcccagcg ggaaagttcc cgcgcggcct tgagccgccg ggccaggatg
      841 gccgcgccat gctcggccaa gggctcgtcc cagaggcgca gctggggatc caggtccgcc
      901 gggcgttggc gcaagagggc gttgcgctgc ttcaaggccc gctcatactg ctggtaatgg
      961 ggcaggaacc ccccgtccag ctgcaggatg gtcaggtcga ggtacttgcg ccgcaacgcc
     1021 ggaccgccct tgaccaagaa aagatcgtcg ggcgagaaga ccaccgcggg catctggccc
     1081 aacagggcgg aaagtttttt ttgttttttc ccgttcaggg tgagcgtctt ccccttgtgc
     1141 ttcctgaatt ccaaaaagac ccggtcctgc cccttttctt tttgaagaag accctccacg
     1201 gcggcgcttt cccggccgaa ctggatcatt tcatcgtcgc cgcccgcgcg gaaggagcgg
     1261 gccgcggaca ggcagtaaag cgcctccagc aggctcgtct tcccctgggc gttcatcccc
     1321 aggatgaggt tcaagcgcgg gtggaagggg acctccaagg aaccgtaatt gcgaaactgg
     1381 gaaagccgaa tggaaccaac atgcaatgct cagccctccc aacgccgtag ggaagggtgc
     1441 agggtcttat tcaggccctt cctaaataat aaagcgcaat cgttcatgga aaacgggccc
     1501 attataaact ttcacggggt ctttttcatt tttaattcgg gcttttcccc cgattttaaa
     1561 atggctttta aaaaaataga ccaataaatt gtgcttttaa aaatgggcct tgggagggac
     1621 tgttccacgt ggaacatcac cggccgggag caaaagttcc acgtggaa
//
LOCUS       DASTJJ010000821         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_481570, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000821 DASTJJ010000000
VERSION     DASTJJ010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481570"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..537)
                     /locus_tag="VFR02_09900"
     CDS             complement(25..537)
                     /locus_tag="VFR02_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9870792.1"
                     /translation="MKTKPKVRPARPGQKITPFLWFDTQAEEAARLYVSLFKNSRITG
                     LTRYGEAGPGPAGSVMSVTFRLAGQPFMALNGGPHFKFTPAVSLFVDCADQKEVDRLW
                     EGLSRGGRKDRCGWLQDKYGLSWQVVPSLLGRLLGDKDRAKAARVMQAMMGMDKLDIA
                     GLKRAAAAKS"
     gene            complement(534..1502)
                     /locus_tag="VFR02_09905"
     CDS             complement(534..1502)
                     /locus_tag="VFR02_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9870793.1"
                     /translation="MTATKNRSNEIRLERVYDAPVQMVWDAWVEPDQVAQWWGPRGFT
                     ITSHSKDIKTGGHWHYTMHGPDGVDYVNKTKYLEVVPHAKMVYDHGGNDEQKPLFRVT
                     VTFQEKAGKTHLDMTMALPTPEAAEETRQIIKRANGNSTWDRLAEYLVKRTSKQEVFV
                     INRSFDAPQALLYDVWTQPDHLAHWMGPKGSKMNIIRRDFRPGGESFYSMSMGEGGPV
                     MYGKLKYLEIAAPERLVYLQQFSDEKGDTARHPMAPTWPETMRTTVLLTPEAEGRTRV
                     TVLWETVDAGAAEIETFVQGRSGMTGGWTGSFDALEEYLGTLGGKP"
     gene            complement(1483..1845)
                     /locus_tag="VFR02_09910"
     CDS             complement(1483..1845)
                     /locus_tag="VFR02_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9870794.1"
                     /translation="MPVAAPDQLSGTFQALADPTRRAMVARLTRGEASVQELAKPFKM
                     SLPAISKHLKVLEKSGLIERGRVAQSRPCRLKTAPIKDAVEWLEQNRKAWEERLDRLD
                     DYLKALQQKEKEHDRHKK"
BASE COUNT          317 a          723 c          665 g          439 t
ORIGIN      
        1 ccagttccac ttgcatgccg ccccctaaga cttggccgcg gcggcccgtt tcagccccgc
       61 gatgtccaat ttatccatcc ccatcatggc ctgcatcacc cgggccgcct tggcccggtc
      121 cttgtccccc agcaaccggc ccaggaggga cggcaccacc tgccaggaca ggccgtactt
      181 gtcctgcaac cacccacaac ggtccttgcg gccgccccgg gaaagcccct cccagagccg
      241 gtccacttcc ttctggtcgg cgcaatcgac gaacagcgag acggcggggg tgaacttgaa
      301 atggggcccg ccgttcaagg ccatgaaggg ctgtcccgcc aggcggaagg tcaccgacat
      361 gacgctcccc gcggggcccg gtcccgcttc cccatagcgg gtcaggccgg tgatccttga
      421 gttcttgaag agcgacacgt agaggcgggc ggcctcttcc gcctgggtgt cgaaccagag
      481 gaagggcgtg atcttttggc cgggtcgggc cggccggacc ttgggcttcg ttttcacggc
      541 ttccccccca gggtgcccag gtactcttcc agcgcgtcga aggaacccgt ccaaccaccg
      601 gtcatgcccg agcggccctg cacgaaggtc tcgatctccg cggcgccggc gtcgaccgtt
      661 tcccagagga cggtcacccg ggtccggccc tccgcctcgg gggtcagaag gacggtggtg
      721 cgcatggtct cgggccaggt gggcgccatc ggatggcggg ccgtgtcgcc cttttcatcg
      781 ctgaactgct ggagatagac gagcctttcg ggggccgcga tctccaggta cttgagcttg
      841 ccatacatca cgggcccgcc ttcccccatg gacatcgaat agaagctctc gcccccgggc
      901 cggaagtccc gccggatgat gttcatcttc gagcccttgg gccccatcca atgggccagg
      961 tggtcgggct gggtccaaac gtcgtacagc agcgcctggg gcgcgtcgaa ggaacggttg
     1021 atgacgaaga cctcctgctt cgaggtgcgc ttcaccaggt actcggccag gcggtcccag
     1081 gtggaattgc cgttggcccg cttgatgatc tggcgcgtct cctcggcggc ctctggggtc
     1141 ggcagcgcca tggtcatgtc caggtgggtc ttccccgcct tttcctggaa ggtcacggtg
     1201 acccggaaga gcggcttttg ctcgtcgttg ccgccgtggt catagaccat tttggcgtgg
     1261 ggcacgacct ccaggtactt ggtcttgttg acgtagtcca cgccgtccgg gccgtgcatg
     1321 gtgtagtgcc aatggccgcc ggtcttgatg tccttgctgt ggctggtgat ggtgaagccc
     1381 cgggggcccc accattgggc cacctggtcg ggctcgaccc aggcgtccca gaccatctgg
     1441 accggggcgt cgtagacccg ctccagccgg atctcgttgg acctattttt tgtggcggtc
     1501 atgctctttc tccttttgct gtaatgcctt caggtaatcg tccaagcggt ccaggcgttc
     1561 ttcccaggcc ttgcggttct gctccagcca ttccaccgcg tccttgatgg gcgcggtctt
     1621 gagccggcag gggcggcttt gggccacccg gccgcgctcg atgaggccgc tcttttccag
     1681 caccttgagg tgcttggaaa tggccggcag gctcatcttg aagggcttgg ccaactcctg
     1741 caccgaggcc tccccccggg tcagccgggc caccatggcc cgcctcgtgg ggtcggccag
     1801 ggcctggaag gtcccgctga gttggtccgg cgcggcgacg ggcatgttgt taacctcttg
     1861 gttatttaac ttaaaggtta tatacaacag ccttccgaaa aggtcaagcg ccttcctgga
     1921 cttttttcga tccccgaccc gcaactttcc ccgggcccgg cccgtcctaa acatgcgcct
     1981 ggatcatcgg gcgtcaaacg gaaagggcgg catgggcctc aaaatccgga aatccgatct
     2041 tctcttattc ttcaccctct tgctggccgt cgcctttcgc cccttggccg tttgcgccca
     2101 ggacatccgt ttggccgcgg gggctccccc ggcccaccac caag
//
LOCUS       DASTJJ010000822         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_972235, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000822 DASTJJ010000000
VERSION     DASTJJ010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972235"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VFR02_09915"
     CDS             complement(<1..1585)
                     /locus_tag="VFR02_09915"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HET9870795.1"
                     /translation="MSVPLDYQWIGDFKDGKAAAIRNGKFGIIDTSGLVVLPFRYDGL
                     GPWSEGRVRMAVGVLYGFIDWGGRTVVEPRYEDVGVFSEGRAWVKQDGRFGFIDAGGR
                     VRVPIRFGAVQDFHDGLAAVQDGGKWGYVDPEGHWVIPARYDDAEQFSDGRAGVWLSS
                     KDEGADFDFIDKKGKAVFSRPYDCEEGLPIFQDGEYREYEYDPVHKSEDWVYYGTQGQ
                     VLRKEPFDPGKTSPATGLPPDLWAEPPASAPPGVWKVRNLQAPFRVVESAPQWGYIDA
                     SGKVQVQPQYDDARPFQEGLAVADQGPDVVWIGPSGKELFRKPRDLYGKNAHFAVGYH
                     EGLIRFMDRGKLLDPGGGGAAPEYDDHYGAADREGNIVLPPIFREMGVPSEGLVFGEV
                     VGDPETEARAAVYRLDGTKAFDDPDWAQEFSEGMAAVRGGYLDTNGKKALEVVDGING
                     SFREGLASLAVGKGAGGTKYGFIDRKGQWAIPPRFCFADPFSEGLAGVQEKEGGKWGY
                     IDHAGNMVLAPRWGWVHDFR"
BASE COUNT          348 a          623 c          469 g          301 t
ORIGIN      
        1 cccggaagtc atggacccat ccccaccggg gcgccaggac catgttcccc gcgtggtcga
       61 tgtaacccca tttcccccct tccttttcct ggacgccggc gagcccttcg gaaaagggat
      121 cggcgaaaca gaagcggggc gggatggccc attgtccctt gcggtcgatg aaaccatact
      181 tcgtccctcc ggcccctttc ccgacggcca gggacgccaa cccttcccgg aaagaaccat
      241 tgatgccgtc aacaacttcc aaggcctttt tcccattggt atccaggtat cctccacgga
      301 cggccgccat cccttcggag aattcttggg cccaatcggg gtcgtcaaaa gccttggtcc
      361 catccaagcg gtaaaccgcc gcccgggcct cggtctccgg atccccgacg acctccccaa
      421 aaaccaaccc ttccgacggc acccccattt cccggaagat cggcggcagg acgatgttgc
      481 cctcccggtc ggccgccccg taatggtcgt cgtattccgg ggcggccccc ccgccgccgg
      541 gatccaggag tttcccccgg tccatgaatc ggatcaatcc ctcgtgatat cccacggcaa
      601 aatgggcgtt cttcccgtaa agatcccgcg gtttccggaa aagctccttc ccggacggcc
      661 cgatccaaac cacatccggc ccctggtcgg ccaccgccag gccctcctgg aaaggcctgg
      721 catcgtcgta ttggggctgg acctggacct ttcccgaagc atcgatataa ccccactggg
      781 gggccgactc caccacccgg aagggggctt gcaggttgcg caccttccaa acgccgggcg
      841 gcgcggaagc cggaggctcc gcccaaaggt cggggggcaa gcctgtcgcc ggggatgtct
      901 tcccgggatc aaagggctcc ttccgcaaga cctggccctg ggtcccgtag tagacccagt
      961 cctcggactt gtggaccgga tcgtattcat attcgcggta ttcgccgtcc tgaaagatcg
     1021 gcagtccttc ctcgcaatca taaggcctgg aaaaaacggc cttccccttt ttgtcgatga
     1081 aatcgaaatc cgccccctcg tccttcgatg aaagccagac gccggcccgt ccgtcgctga
     1141 actgctcggc gtcgtcataa cgggcgggaa tgacccaatg cccctccggg tccacatatc
     1201 cccatttgcc gccgtcctgg acggcggcca acccatcatg gaaatcctgg acggccccga
     1261 accggatcgg gaccctcacc ctacctccgg cgtcgatgaa cccgaaccgc ccatcctgtt
     1321 ttacccaagc ccgcccctcg gaaaagactc cgacgtcctc gtatcgcggt tccaccacgg
     1381 tccgtccccc ccaatcgatg aaaccataga gcacccccac cgccatacgg acccggcctt
     1441 ccgaccaggg acccaggccg tcatatcgga aaggaaggac caccaggccc gaggtatcga
     1501 tgatcccgaa cttcccgttc cggatggccg ccgccttccc atccttgaaa tccccgatcc
     1561 attggtaatc aagggggacg ctcaatcgac ccgaggcgtc catcaccccg aaagactggc
     1621 ggcaaaaagg gaaaaggggg acctgggggc cggaagcccg gcagaccggc ggttggaaag
     1681 cgagacccaa gaccatcccc aagaggatct tgaaaaaccg ggaaggtttg agccggtttg
     1741 g
//
LOCUS       DASTJJ010000823         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_972286, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000823 DASTJJ010000000
VERSION     DASTJJ010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972286"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFR02_09920"
     CDS             <1..257
                     /locus_tag="VFR02_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870796.1"
                     /translation="APAEGRVEFWVKDEGPGSPEEAQGRVFERFFRVDASRSREAGGT
                     GLGLSIVKHLVDRMNGDIKLESREGKGSTFRILLPAPPEA"
     gene            complement(259..1041)
                     /locus_tag="VFR02_09925"
     CDS             complement(259..1041)
                     /locus_tag="VFR02_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HET9870797.1"
                     /translation="MKTKRFYGKGLPNIDPTQLKGTLIVLEGSDGSGRSTQIGLLQNW
                     LERRGYPTVVVGLKRSDLVGEALAEAMRGNTLNAITLNLFYATDFADQLERVMIPALK
                     AGFVVLADRYIYTLMARDIVRGSSLEWLKEIYGIALVPDAVFYLNVNPRTLAERNFRK
                     RGYLDYWESGMDIQRVGGMYDNFIKYQAKLHRIFEHLQKEFAFSTIHGGKSVRSVFKE
                     IRTRVEKILAASAAAEKAAPAPEPPPAPEAAPPAKGEDAPAK"
     gene            complement(1038..1727)
                     /gene="tmk"
                     /locus_tag="VFR02_09930"
     CDS             complement(1038..1727)
                     /gene="tmk"
                     /locus_tag="VFR02_09930"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9870798.1"
                     /translation="MKTSKKEIEGKLIAVEGLDGSGKSTQIRLLKRWLELEGYKVFFT
                     EWNSSDLVKRATRKGKKKKLLTPTTFSLIHATDFADRYERQILPLLKGGYIVLADRYV
                     YTAFARDAVRGCDKDWLRKLYRFARKPDITFFFEVPLEVALGRILEGRPSLKYHEAGM
                     DMGFSKDPYECFKIFQGRIYEEYGRIFKEFPAERVNATEPSELQQSKMRDIVKKRIDL
                     KAFKLKTQGVL"
     gene            complement(1771..>1997)
                     /locus_tag="VFR02_09935"
     CDS             complement(1771..>1997)
                     /locus_tag="VFR02_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9870799.1"
                     /translation="CPIVIRYQNEVVGEYTADLLVENRVLVELKATEKLSSIHSAQCL
                     NYLKATGLSACLLMNFGELKLKIRRLTHHL"
BASE COUNT          345 a          582 c          662 g          408 t
ORIGIN      
        1 ccgcgcccgc ggaggggcgg gtggagttct gggtgaagga cgagggcccg ggcagccccg
       61 aggaggccca gggccgggtc ttcgagcgtt tcttccgggt ggacgcctcc cgcagccggg
      121 aagcgggggg cacgggactg gggctttcca tcgtcaagca cctggtggac cgcatgaacg
      181 gcgacatcaa gctggaaagc cgggagggga agggcagcac cttccgcatc ctgctgccgg
      241 cgccccccga ggcttgagct acttggccgg ggcgtcctcg cccttggccg ggggcgcggc
      301 ctccggggcg ggcgggggtt cgggcgccgg ggcggctttt tcggccgccg ccgaggcggc
      361 caggatcttc tccacccggg tgcggatctc cttgaacacc gaccgcacgg acttgccgcc
      421 gtggatggtg gagaaggcga attccttctg caggtgctcg aagatgcggt gcagcttggc
      481 ctggtacttg atgaagttgt cgtacatgcc gcccacccgc tggatgtcca tccccgattc
      541 ccaatagtcc aggtacccgc gcttgcggaa gttgcgctcg gccagcgtcc tgggattgac
      601 gttcaggtag aagaccgcgt ccgggaccag ggcgatgccg tagatctcct tcaaccattc
      661 cagcgaggag ccccgcacga tgtcgcgggc catcagggtg tagatgtagc ggtcggccag
      721 caccacgaaa ccggccttga gggcggggat catgacccgc tccaactggt cggcgaagtc
      781 cgtggcgtag aaaaggttca gggtgatggc gttgagggtg ttgccccgca tggcctcggc
      841 caacgcctcg cccaccaggt cggagcgttt caggcccacc accacggtgg gatagccccg
      901 gcgctccagc cagttctgca gcagcccgat ctgggtcgag cggccgctgc cgtcggagcc
      961 ttccagcacg atcagcgtgc ccttgagctg ggtcgggtcg atgttgggca ggcccttgcc
     1021 gtaaaagcgc ttggttttca taggaccccc tgggtcttga gtttgaaggc cttcaggtcg
     1081 atgcgcttct tcacgatgtc gcgcatcttg gactgctgga gctcggaggg ttcggtggcg
     1141 ttcacccgtt cggcggggaa ctccttgaag atgcggccgt attcctcgta gatccgccct
     1201 tggaagatct tgaagcactc gtaggggtcc ttggaaaagc ccatgtccat gccggcctcg
     1261 tggtatttca gggacgggcg gccctccagg atgcggccca gggccacctc caggggcacc
     1321 tcgaagaaga aggtgatgtc gggtttgcgg gcgaaacggt agagtttgcg cagccagtcc
     1381 ttgtcgcaac cccgcacggc gtcccgggcg aaggccgtgt agacgtaacg gtcggccagc
     1441 acgatgtagc cgcccttcaa gagcggcagg atctggcgct cgtagcggtc ggcgaagtcc
     1501 gtggcgtgga tcagggagaa agtggtgggg gtcaacagct tcttcttttt gcccttgcgg
     1561 gtggcccttt tcaccaggtc cgaggagttc cattcggtga agaagacctt gtagccttcc
     1621 agttccagcc agcgcttcaa cagccggatc tgggtggatt tgcccgatcc gtccaggccc
     1681 tccacggcga tgagcttgcc ttcgatttct ttttttgagg ttttgatcgg cacggaatga
     1741 ctccttcttg gaacaatgat gaaaagctga tcaaagatga tgggtcaaac gccttatttt
     1801 taacttcaat tcgccgaagt tcatcagcag gcaagccgag agtccggtgg cttttaagta
     1861 gttcaagcac tgcgcggaat ggatcgagct tagcttttcc gtcgccttca attcgaccaa
     1921 aacccggttt tcgaccagca agtcggccgt atattcaccg actacctcgt tttgataacg
     1981 aatgacgatg gggcatt
//
LOCUS       DASTJJ010000824         1321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_359416, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000824 DASTJJ010000000
VERSION     DASTJJ010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359416"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFR02_09940"
     CDS             complement(<1..833)
                     /locus_tag="VFR02_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870800.1"
                     /translation="MAPVFANVPGGGTGTGGNVTVTDNGGTVVLANGIVSFTINKSSG
                     TITNFTYNGVNLLSGGHGGGSFYFDGSGGPTLSGTVYTLTVNPASNGGNQAEIWLQST
                     ASPIDMAVYYDLLRGQQGIYDTMILTHEAGYPDYPGAELRTNMYVGSMFDWLCVDPYR
                     FRRMADPNSTSVAVTGAPPEVFQWTSGIYSGLTECKYAYSAPLGKVDVYGWASSTQPY
                     GVWQTFPSHEYLDGGPMHRELTEHIGNTLLDMFGGGHYGFGSNGDLPAGDYESKTFGP
                     A"
BASE COUNT          248 a          400 c          436 g          237 t
ORIGIN      
        1 aaggccgggc cgaaggtctt ggactcgtag tcgcccgcgg gcaggtcgcc gttggaaccg
       61 aagccgtagt gcccgccccc gaacatgtcc agcagggtgt tgccgatgtg ctcggtgagt
      121 tcccggtgca tgggcccgcc gtccaggtac tcgtggctgg ggaaggtctg ccagacgccg
      181 tagggctggg tcgaggaagc ccagccgtag acatccacct tgcccagggg cgcgctgtag
      241 gcgtacttgc attcggtcag ccccgaatag atgccgctgg tccactgaaa gacctccggg
      301 ggggcccccg tcacggccac cgaggtggag ttggggtcgg ccatccgacg gaagcggtag
      361 ggatccacgc aaagccagtc gaacatcgac cccacgtaca tgttggtgcg cagttcggcc
      421 cccgggtagt ccgggtaacc ggcctcgtgg gtgaggatca tggtgtcgta gatgccctgc
      481 tggccccgga gcaggtcgta atagacggcc atgtcgatgg ggctggccgt ggactggagc
      541 catatttcgg cctggttgcc gccgttggac gcggggttga cggtgagcgt atagaccgtg
      601 cccgaaaggg tgggcccgcc cgatccgtcg aagtagaagg aaccgccgcc gtgccccccg
      661 gaaagcaggt tcacaccgtt gtaggtgaag ttggtgatgg tgccgctgct cttgttgatg
      721 gtgaaggaaa cgatgccgtt ggccaacact acggtgccgc cgttgtcggt cacggtgacg
      781 ttgccgcccg tgcccgtgcc cccgccgggc acgttggcga aaaccggtgc caagcccgct
      841 ccggccagga gggccaaggc taagatcgtt ttcaaaaaga agcgcatggt tccccgtcgt
      901 caaacgatgg aagcgccgtc cattggagga cggacaagaa aagtctatcg ggacccgcca
      961 tggaaaggcc caataatcgc ccttattttg gtaaaaccat ggacccgtcc attgaaccga
     1021 ttcagggttt cccgaccggg cgccaaatcc ctaggactta gtccccctga ccggggggtt
     1081 tattccctct ttctgtccgc ttgtgacgaa taaaaaaacc gtccccaaag ggacggcctt
     1141 gcggcgatcc aagacgcgct gcccgcggcc tggccacaag ccaggccgct ctcttgggcg
     1201 tgccggaaac tacaggttcc gacctaaggc tggtggcctg cctgcgttga ggcctcaagc
     1261 cgaaacgcat gacttgacgt gtcggggcct accggccccg acctcagtgc aggaagcgac
     1321 a
//
LOCUS       DASTJJ010000825         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_602239, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000825 DASTJJ010000000
VERSION     DASTJJ010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602239"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..881)
                     /locus_tag="VFR02_09945"
     CDS             complement(78..881)
                     /locus_tag="VFR02_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870801.1"
                     /translation="MVRIAKGIAWLGLVLSVGIFTGCGAPRVYPPAFSLYGYQRIVVV
                     PFDDQTQDPALGITLQSEMEDQVLSLNAVPLVDAKQTAAYLKGLKADPASVATDPELM
                     KKIAQKFKADLLLVGAATGYVEILKDEAPQQDSSGHWGFNTFRKVSVAGKAQLLDPAS
                     GSLVWSGKNQGYSYTNTFNPLPIPAGVRLPAGLDGVLGMANLVKNRVLGKDDREPATL
                     DENDPNVLLYPNSHYFAHLREEATYELVNAMVDDFRGHNGWTPGMAASR"
     gene            1019..2026
                     /locus_tag="VFR02_09950"
     CDS             1019..2026
                     /locus_tag="VFR02_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9870802.1"
                     /translation="MGILLSCQNLTKSFGHRPLFEDLSFGLFEGERTGLIGPNGSGKS
                     TLVKILAGLEKPDSGLLSLRKGLRVGYLAQQDNFAEQAGGLDVQGALRRALEGLGLED
                     YEVDMRVDAGLEEAGFPDPAQPVAALSGGWRKRLAILSQVLREPDLLLLDEPTNHLDL
                     EAVLWLENYMQTLDFSFLVVTHDRRFLERVSNRVLELNKQYPNGYFSNAGNYSQFLEK
                     REDLFNAQARREDTVANTVRREIEWLRRGPKARQTKQQARIDRAGDLMGELDELQYRN
                     SQGKSVDIDFSGSGRQTKRLVAAEKVVKSLGGRKLFGPLDLLMTPGDKLGLLGSNGSG
                     K"
     gene            2030..2836
                     /locus_tag="VFR02_09955"
     CDS             2030..2836
                     /locus_tag="VFR02_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9870803.1"
                     /translation="MLKILAGRLAPDGGTLKQAEQLRVVVFDQHRGQLELDQPLRRAL
                     CETGDYVHYKGSPIHVASWAARFLFSKDQLDRRLREFSGGEQSRVLIARLMLQPADLL
                     LLDEPTNDLDIPSLEVLEQSLVEFPGALVLVTHDRYLLDRVSQRILALDGKGHADYFA
                     DLAQWETSREQGTGQQKAPAPVAAPPPAAPKKSPLSSKEKKELEGMDASLQAAEAELK
                     AAQARLEDPAVAADVAELLKRQKALDAAKAKQDSLLARWEELEAKKGQAS"
     gene            complement(2973..>3280)
                     /locus_tag="VFR02_09960"
     CDS             complement(2973..>3280)
                     /locus_tag="VFR02_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9870804.1"
                     /translation="QANLPVVDLARTLATLPVLALHLKPSLPLPSPAFRWAWDHFQRD
                     GGYGVTMFFVISGFLITRTLDLRPGGLFRSTWRGFYSRRAGRILPLFLVDILLGAGR"
BASE COUNT          604 a         1083 c         1066 g          527 t
ORIGIN      
        1 gaggctggtg ctgtggcccg tcccctggcg tgatcgggac cggccctggc ggccgcccgc
       61 ccgaagggac taggccctca ccgcgacgcc gccatgcccg gcgtccatcc attgtggccg
      121 cggaagtcgt ccaccatggc gttcaccaac tcgtaggtgg cctcctcgcg caggtgggcg
      181 aaataatggc tgttggggta cagcagcaca ttggggtcgt tctcgtccag ggtggcgggt
      241 tcccggtcgt ccttgcccaa cacccggttc ttgaccaggt tggccatgcc caggaccccg
      301 tccaagccgg cgggcaaccg cacgcccgcg gggatgggca aggggttgaa ggtgttggtg
      361 tagctgtaac cctggttctt cccgctccag accaggctgc cgctggcggg gtcgagcaat
      421 tgcgccttgc ctgccacgga caccttgcgg aaggtgttga acccccaatg gccgctggag
      481 tcctgctggg gcgcttcatc cttcaatatc tccacgtagc cggtggcggc ccccaccaac
      541 agcaggtcgg ccttgaactt ttgggcgatc ttcttcatca gttcggggtc ggtcgcgacc
      601 gaggccgggt cggccttcag ccccttcagg taggcggccg tttgcttggc gtcgaccaag
      661 ggcacggcgt tgagggaaag gacctggtcc tccatctcgg actgcagggt gatccccagg
      721 gcggggtcct gggtctggtc gtcaaaaggc accaccacga tccgttggta cccgtaaagg
      781 ctgaaagcgg gcggatagac cctcggcgcc ccgcaaccgg tgaagatccc gacggataag
      841 accaggccca accaggcgat tcccttcgcg atgcggacca tcgttgcccc cttgaccggc
      901 caggatgaaa ggcggggcca ttataccccc gggggaagta acggccctcc cccgatttta
      961 ggcttgtttt tggccctttc gcccttatcc ttacccttcc atttgaacaa aggaacccat
     1021 gggcatcctc ttaagctgtc aaaacctgac caaaagtttc ggccaccggc ccctcttcga
     1081 agacctgtcc ttcggcctct tcgagggcga acgcaccggc ctcatcggcc ccaacggctc
     1141 gggcaaatcc accctggtca agatcctggc cggcttggaa aaacccgaca gcggcctctt
     1201 gtccctgcgc aagggcctgc gggtgggcta cctggcccaa caggacaatt tcgccgagca
     1261 agccgggggg ctggacgtgc agggggccct gcgccgcgcc ctggagggcc tgggcctgga
     1321 ggactacgag gtggacatgc gggtggacgc cgggttggag gaagcgggct tccccgaccc
     1381 cgcccagccc gtggccgccc tctcgggcgg ctggcgcaag cgcctggcca tcctgtccca
     1441 ggtgttgcgg gagcccgacc tgctcctgct ggacgagccc accaaccacc tggacctgga
     1501 agccgtcctg tggctggaga actacatgca gaccctggac ttttcgttcc tggtggtcac
     1561 ccacgaccgc cggttcctcg agcgggtcag caaccgggtg ctggagctca acaagcaata
     1621 cccgaacggc tatttcagca acgccggcaa ctacagccag ttcctggaaa aacgggagga
     1681 cctcttcaac gcccaggccc gccgcgagga cacggtggcc aacaccgtgc gccgggagat
     1741 cgagtggctg cgccgggggc ccaaggcccg ccagaccaag cagcaggccc gcatcgaccg
     1801 ggccggggac ctgatgggcg aactggacga gctgcagtac cgcaactccc agggcaagag
     1861 cgtggacatc gatttctccg gcagcggccg gcagaccaaa cgcttggtgg cggccgagaa
     1921 ggtggtgaaa agcctgggcg gccgcaagct cttcggcccg ctggacctgt tgatgacgcc
     1981 cggcgacaag ctggggcttt tggggtccaa cggcagcggc aagtagacct tgctcaagat
     2041 cctggcgggg cgcctggcgc ccgacggggg cacgctcaag caggccgagc agctgcgggt
     2101 ggtggtcttc gaccagcacc ggggccagtt ggagctggac cagcccctgc gccgggcctt
     2161 gtgcgagacc ggcgactacg tgcattacaa gggctccccc atccacgtgg ccagttgggc
     2221 ggcccgcttc cttttctcca aggaccagtt ggaccggcgc ctgcgggaat tctccggggg
     2281 cgaacaatcc cgggtcctca tcgcccggct gatgctccaa cccgccgacc tgctgttgtt
     2341 ggacgagccc accaacgacc tggacatccc ctccctggag gttttggagc aaagcctggt
     2401 ggagttcccc ggcgccctgg tgctggtgac ccacgaccgt tatctcctgg accgggtcag
     2461 ccagcgcatc ctggccctgg acggcaaggg ccacgcggat tatttcgccg acctggccca
     2521 atgggaaacg tcccgggaac aggggaccgg gcagcaaaag gcccccgctc ccgtggccgc
     2581 cccgccgccc gccgccccca aaaagagccc gctcagttcg aaggaaaaaa aggaactgga
     2641 gggcatggac gcctccctcc aagcggccga agccgagctg aaggccgccc aggcaaggct
     2701 ggaagacccg gccgtggccg ccgacgtcgc ggagttgctc aagcgccaaa aggccctcga
     2761 cgccgccaag gccaagcagg attccctctt ggcccgctgg gaagaattgg aagccaagaa
     2821 gggccaagct tcttaaagga cttttctcaa aagcaaaaat caaatctttt caccgcagag
     2881 acgcggagac gcggcgaaaa accatcattt ccaccgtttt gaccgtccct caactttgcg
     2941 tctttgcggt gaaccaggtt ttggatcggc ttttagcggc ccgcccccaa caggatgtcg
     3001 acgaggaaca ggggcaggat gcgccccgcc cggcgggaat aaaaaccccg ccaggtcgac
     3061 cggaaaagcc cgccggggcg caggtccaaa gtgcgggtga tgaggaagcc cgagatcacg
     3121 aagaacatcg tgacgccgta accgccgtcg cgctggaaat ggtcccaggc ccagcggaaa
     3181 gccggtgacg gaaggggcag gctgggcttc aggtggagcg ccaggaccgg cagggtggcc
     3241 agggtccggg ccaggtccac caccggcagg ttggcctggg
//
LOCUS       DASTJJ010000826         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_482197, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000826 DASTJJ010000000
VERSION     DASTJJ010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482197"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFR02_09965"
     CDS             <1..806
                     /locus_tag="VFR02_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9870805.1"
                     /translation="SPKSVAAEMEICVEKYGVWEFFDDNESGPIYNKEWLKEFHKELT
                     ERKLLGRFQFSSNCRADNLNEEIVDLLVKCGWRLLKIGLESGSDYSIKHLAKCETVEL
                     ISRGVKMAKDRGLRVLLTTMMGYPWETEEDVRKTYEVTKDLLLYKVRAGDCMQASVVI
                     AYPGSPLWREATRKGWLLIGEKDYEKYDMSHPILKTPINASAWATKTWNLMKHPKFVM
                     GQVVTVRSPREVDLLWRGLQSLLGHVQDYGDPLPESAPAHATPQTVASR"
     gene            775..1428
                     /locus_tag="VFR02_09970"
     CDS             775..1428
                     /locus_tag="VFR02_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870806.1"
                     /translation="MPHPKPSHPVEPTSRSPRSGFTPGRSAVLALLALVLVAAALGAG
                     GLWQDLFAGPKIPLPRQNGPLTLGMPEAQVAQLLPGSRKKLRPFNNDPDFKIADLTSA
                     DGLPTGWSTLSLLYYQGRLYYLSVMWNGDAAQALPFDQWVRQYRRWDRGPQGHQTQSM
                     GAQITLEEWHFTDGSTEMTLRNLGYDGKTQRWQDLRDSSDAAAQAAFSKYRLDGGAR"
     gene            1444..2409
                     /locus_tag="VFR02_09975"
     CDS             1444..2409
                     /locus_tag="VFR02_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9870807.1"
                     /translation="MRRLGSFTFILGLVLFAVLLGRVDLPTSWRILTSARPLWTLLAF
                     LFLLPEVFFKAWRIKVLSHFFHSRLTFAQGNWIYLSGQPLSAVTPAKLGDIVRVLGIR
                     HWGGLKLTGAFAVHVADKVYDLLALGLFAASGLMVALAQHENQTPALMALVGLALGLL
                     AMALFLNPQWMRSFIRPALLALAPQKLAKQLRTHGSEFYADLLSIFRSFNRVLLPFTL
                     SLGAWFTVWVRAYFCALALGIPLSFGRILLLMPVVVVIEFIPVTLMGFGTREAALFLF
                     FASAAVSKSALLSFSLLNVLAGPLFTSLVGIPCAWRLGAHLERKS"
     gene            2406..3422
                     /locus_tag="VFR02_09980"
     CDS             2406..3422
                     /locus_tag="VFR02_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9870808.1"
                     /translation="MKPPYSVVIASARADRGLESVAALLRLPAAERPAEIIVCVGRNP
                     SRQRNLGVALCQSPLVYFLDDDSRTVAATPRRLAAHFRDPRTAVAGGPNLHPPGATPF
                     ERTVSAVLASWLGSFKVRSRYASVGSLREATEKDLILCNLMVRRSTFQKLGGFRVDLY
                     PNEENEFLNRLLHGGAQLLYDPGAVVYRHRRKNLGAFCHQAFRYGQGRARQIKVYPCL
                     SDLVHLIPAFFVLYTLSLAAAAFPAAPAFLRTPFWWAPFALFWALALGTSLSAFSWNR
                     QIWDIGAVPVLIFLRQFFYGVGLAAGFLRPSPPASQAPAAAYRVKARGTSYRLVPVKR
                     PKGG"
     gene            3426..>3945
                     /locus_tag="VFR02_09985"
     CDS             3426..>3945
                     /locus_tag="VFR02_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870809.1"
                     /translation="MTPDEKPSRAWTAPLLFLAVAFVAFFPGLVLGRYYFANDLLAFS
                     HGHLGLLKHDFAQGHLSLWNPFLFGGQPIFANPNYMRCNPLNYLIPLFPIPYGLTVYF
                     FLHLFLAAFGAYLLLKTLRCSEGASRMGALLYGLSGDFWWEIIHPSILTVLALWPWLA
                     LTLETLSREWRPR"
BASE COUNT          564 a         1467 c         1171 g          743 t
ORIGIN      
        1 ggtcgcccaa gagcgtggcc gccgagatgg agatctgcgt ggaaaaatac ggggtctggg
       61 agttcttcga cgacaacgag tccggcccca tctacaacaa ggaatggctg aaggaattcc
      121 ataaagaact caccgagcgc aagctcctgg gccgcttcca gttctcctcc aattgccggg
      181 cggacaacct gaacgaggag atcgtcgact tgttggtgaa gtgcggctgg cggctcctga
      241 agatcggcct ggagtcgggg tccgactaca gcatcaagca cctggccaag tgcgagacgg
      301 tggagctcat cagccggggc gtcaagatgg ccaaggaccg gggcctgcgg gtgctgttga
      361 ccaccatgat gggctatccc tgggagaccg aggaggacgt ccgcaagacc tacgaggtca
      421 ccaaggacct gcttctctat aaggtccggg ccggggactg catgcaggcc tcggtggtca
      481 tcgcctaccc gggctcgccc ctctggcgcg aggccacccg caagggctgg ctcttgatcg
      541 gcgagaagga ctacgagaag tacgacatga gccatccgat cctcaaaacg cccatcaacg
      601 cctccgcgtg ggccaccaag acctggaacc tgatgaagca ccccaagttc gtgatggggc
      661 aagtcgtgac cgtgcgttcc ccgagggagg tggacctgct ctggcggggc ctgcagtccc
      721 tcctggggca cgtgcaggat tatggggacc cactcccgga aagcgcgccc gcccatgcca
      781 caccccaaac cgtcgcatcc cgttgagccc acctcccgga gcccgcgttc cggcttcacc
      841 cctggccgat ccgcggtcct ggccctcctg gccctcgtcc tggtggccgc cgcgctcggc
      901 gcggggggcc tctggcagga cctcttcgcg ggccccaaga tccccctgcc ccgccagaac
      961 ggccccctga ccctggggat gcccgaagcc caggtggccc aattgctgcc cgggtcccgg
     1021 aagaaactgc gccccttcaa caacgacccg gacttcaaga tcgcggacct gacctccgcc
     1081 gacggcctgc ccacgggctg gtccaccctc tcgctgctct attaccaggg gcggctttac
     1141 tacctttccg tgatgtggaa cggggacgcg gcccaggccc tgcccttcga ccaatgggtg
     1201 cgccaatacc ggcgctggga ccggggcccc caggggcacc agacccagtc catgggcgcc
     1261 cagatcacgc tggaagaatg gcatttcacc gacggctcca ccgagatgac cctccgcaac
     1321 ctgggctatg acggcaagac ccagcgttgg caggacctgc gggattcctc cgacgccgcc
     1381 gcccaggccg ccttctccaa ataccgcctg gacggcggcg cccggtaacg gtcggcccct
     1441 cccgtgcgac gcctcggttc cttcaccttc atcctggggt tggtcctctt tgcggtcctc
     1501 ttgggccggg tggaccttcc cacctcgtgg cgcatcctga cctccgcccg gcccctctgg
     1561 accctgctgg ccttcctctt cctgctcccc gaggtcttct tcaaggcctg gcggatcaag
     1621 gtgctctccc actttttcca ttcccgcctc accttcgccc agggcaactg gatctacctc
     1681 tcgggccagc ccctctcggc ggtgacgccc gccaagctgg gggacatcgt gcgggtcttg
     1741 ggcatccggc attggggagg cctcaagctg accggcgcct tcgccgtgca cgtggccgac
     1801 aaggtctatg acctcttggc cctgggcctg ttcgccgcct ccggattgat ggtggccctg
     1861 gcccagcacg agaaccagac ccccgccctg atggccctgg tgggcctggc cctggggctt
     1921 ttggccatgg ccttgttcct caatccccag tggatgcgtt ccttcatccg ccccgcgctc
     1981 ctggccctgg ccccccagaa gctggccaag cagctgagga cccacggctc cgagttctac
     2041 gccgacctgc tttccatctt ccggtccttc aaccgggtcc tgctgccctt caccctgtcc
     2101 ctgggggcct ggttcacggt ctgggtgcgg gcctattttt gcgccctggc gctgggcatc
     2161 ccgctttcct tcgggcgcat cctgctgctc atgcccgtgg tggtggtcat cgagttcatc
     2221 cccgtgaccc tgatgggctt cggcacccgg gaagccgccc tgttcctctt cttcgcttcg
     2281 gccgccgtct ccaaatcggc cctgctttcc ttctcgttgc tgaacgtatt ggcgggcccc
     2341 ctcttcactt ccctggtggg catcccctgt gcctggcggt tgggcgccca cctggagcgc
     2401 aagtcatgaa acccccctac agcgtggtga tcgcctccgc ccgggccgac cggggcctgg
     2461 aaagcgtggc ggccctgctg cgcctgccgg cggccgaaag gcccgccgag atcatcgtct
     2521 gcgtggggcg caacccgtcc cgccagcgca acctgggcgt ggccctctgc caaagccccc
     2581 tggtctattt cctcgacgac gacagccgca ccgtggcggc caccccccgg cgcctcgccg
     2641 cccatttccg ggatccccgc acggcggtgg ccggcggccc caacctgcac ccgcccggcg
     2701 ccacaccctt cgaacgcacc gtcagcgccg tgctggcctc ctggctgggc agcttcaagg
     2761 tgcgcagccg ttacgcgtcc gtggggtcct tgcgggaggc caccgagaag gacctgatcc
     2821 tctgcaacct gatggtgcgc cgctccacct tccaaaagct cggcggcttc cgggtggacc
     2881 tctaccccaa tgaggagaac gaattcttga accgcctgct gcacgggggc gcccagctcc
     2941 tttatgaccc gggcgcggtg gtctaccgcc accggcgcaa gaacctgggg gccttctgcc
     3001 accaggcctt ccggtacggc cagggccggg cccggcagat caaggtctat ccctgcctgt
     3061 cggacctggt gcacctgatc ccggccttct tcgtgctcta taccctgtcc ctggccgccg
     3121 ccgccttccc ggccgcgccc gctttcttaa ggaccccctt ctggtgggcg cccttcgccc
     3181 ttttctgggc cctggccctg ggcacctccc tttcggcctt ttcctggaac cggcaaatat
     3241 gggatattgg ggccgtgccc gtcctgatct tcctgcggca gttcttctac ggggtgggcc
     3301 tggcggcggg cttcctgcgc cccagccccc cggcgtccca ggcccccgcg gcggcctacc
     3361 gggtaaaagc ccggggaacc tcctaccggc tggtcccggt gaaaagaccg aaaggcggat
     3421 aacgaatgac accggacgag aagccctccc gcgcttggac cgcgccgctg ctcttcctgg
     3481 ccgtggcctt cgtggccttc ttcccgggcc tggtcttggg ccgttattat ttcgccaacg
     3541 acctcttggc cttcagccac ggccacctgg gcctgctgaa acacgacttc gcccaggggc
     3601 acctgtcgct ttggaacccc ttcctgttcg gcggccaacc catcttcgcc aaccccaact
     3661 acatgcgctg caaccccttg aactacctga tcccgctctt ccccatccct tacggcctga
     3721 cggtctattt tttcctgcac ctgttcctgg cggccttcgg cgcctacctg ctgctcaaga
     3781 cgctgcgctg ttcggagggg gccagccgga tgggcgccct gctctacggc ctctcgggcg
     3841 acttctggtg ggagatcatc catccctcca tcctcaccgt gctggcgctc tggccctggc
     3901 tggccctgac cctggagacc ctcagccggg aatggcgccc gcgcg
//
LOCUS       DASTJJ010000827         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_972639, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000827 DASTJJ010000000
VERSION     DASTJJ010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972639"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1529
                     /locus_tag="VFR02_09990"
     CDS             <1..>1529
                     /locus_tag="VFR02_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9870810.1"
                     /translation="GQEKTVKLLDDIKQTGFKYASKAGITVSIDDMKIPKDKVEILKR
                     SYKDVAEIENQYKKGIITAGERYNKVIDIWTHTTDEIAEVMFENLKKDQYGFNPIFMM
                     ADSGARGSKMQIRQLAGMRGLMAKPLQKITGSIGEIIESPITANFREGLTVLEYFIST
                     HGARKGLADTALKTADAGYLTRRLVDVAQEVIINIEDCGTNNGIRVSAIKEGLEIIEP
                     LKDRIAGRVAVDNVANLLTGEVLVKSGQAITEPIAEKLEEAGIEQIRVRSVLTCELER
                     GVCAKCYGVNLANGREVQIGEAVGIIAAQSIGEPGTQLTLRTFHVGGAASRALEQSKI
                     EAGKSGRLKFHNLRLVENRNKELVVLNRNGEITINDDKGRTLETHTVPFGSTLRFKEG
                     DAVKESQLVAAWDLYKVPIFTEVSGVVKLQDVIRGVTMREEIDSSTGLKERAIIEYKD
                     ENLSPRCVIVNPDTGEALKSYRLPTGSLINVSEGDKVMAGDVLSKLPRQMSKTRDITG
                     G"
BASE COUNT          341 a          484 c          484 g          220 t
ORIGIN      
        1 gggccaggaa aagaccgtga agctgctgga cgacatcaag cagaccggtt tcaagtacgc
       61 cagcaaggcc gggatcacgg tgtccatcga cgacatgaag atccccaagg acaaggtcga
      121 gatcctcaag cgttcctaca aggacgtggc cgagatcgag aaccagtaca agaagggcat
      181 catcacggcc ggcgagcgct acaacaaggt catcgacatc tggacccaca cgaccgacga
      241 gatcgccgag gtcatgttcg agaacctcaa gaaggaccag tacggcttca accccatctt
      301 catgatggcg gattccgggg cccgcggttc caagatgcag atccgccagt tggccggcat
      361 gcgcggcctg atggccaagc ccctgcagaa gatcacgggc tccatcggtg agatcatcga
      421 atcgcccatc acggccaact tccgggaggg gctgaccgtg ctggaatact tcatctccac
      481 ccacggcgcg cgcaagggtt tggccgacac ggccttgaag accgccgacg ccggttacct
      541 gacccgccgc ctggtggacg tggcccagga agtcatcatc aacatcgagg attgcggcac
      601 caacaacggc atccgggtct cggccatcaa ggaaggcctg gagatcatcg agcccttgaa
      661 ggaccgcatc gcgggccggg tggccgtgga caacgtggcc aacctgctca ccggggaggt
      721 cctggtcaag agcggccagg ccatcaccga gcccatcgcg gaaaaactgg aggaggcggg
      781 catcgagcag atccgggtcc gttcggtcct cacctgcgag ctggaacggg gcgtctgcgc
      841 caagtgctac ggcgtgaacc tggccaacgg ccgggaagtg cagatcggcg aggcggtggg
      901 catcatcgcc gcccagtcca tcggcgaacc gggcacccag ctgaccctgc gcaccttcca
      961 cgtgggcggc gccgccagcc gcgccttgga acagtccaag atcgaggcgg gaaaaagcgg
     1021 ccgcctgaag ttccacaacc tgcggttggt cgagaaccgc aacaaggaat tggtggtcct
     1081 caaccgcaac ggcgagatca ccatcaacga cgacaagggc cggacgttgg agacccatac
     1141 ggtgcctttc ggatccaccc tccgcttcaa ggaaggcgac gcggtcaagg aaagccagct
     1201 ggtcgccgcc tgggacctgt acaaggtccc catcttcacc gaagtgagcg gcgtggtgaa
     1261 gctgcaggac gtgatccgcg gcgtgaccat gcgcgaggag atcgactcct cgaccggcct
     1321 caaggaacgc gccatcatcg agtacaagga cgagaacctc tcgccccgtt gcgtgatcgt
     1381 gaaccccgac acgggggagg cgctcaagag ctaccgcctg cccacgggtt cgttgatcaa
     1441 cgtgtccgag ggcgacaagg tgatggccgg ggacgtgttg tccaagctgc cccgccagat
     1501 gtccaagacc cgcgacatca cgggcggcc
//
LOCUS       DASTJJ010000828         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_847356, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000828 DASTJJ010000000
VERSION     DASTJJ010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847356"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2136
                     /locus_tag="VFR02_09995"
     CDS             <1..>2136
                     /locus_tag="VFR02_09995"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HET9870811.1"
                     /translation="LAPGQYRVTVKMVNLTYLQPSQDVFNIFANGTEVVPNLDLFESA
                     DYGQAFDVTFTVQVDASADWLESNAGVGTLDLLASASKGTATVSAIEVVGLQAQPPPL
                     PTRTPVPGALDFRLNAAGPAVTDSMGALWTADQFFGSATGVPGLGAFGWTQGVTEVTV
                     PDGFVGNHSTPDPALYETYREDRTVGYRFAVPNGLYQVTFKWAELEYDLPGQRIFNVL
                     AQGQTLAPRVDLDTTVNFGDAFDQTFYDIPVTNGVLDVSFQALPGSLGGGMVSAIHVA
                     GEQPIPSATPTGTPTNSPTVTNSPTATGTPTDTATLTPTPVGTWYTATPTSTATATPT
                     PLPPDAELLKLQELPTLPHPNPTAVDAEGARVTAPVSVIGTANGNFAPAPPNRGYQSG
                     WFLELQPLSPAGASAVTLASGTAQVTNGPLGVLDPTLLLNGAYQLELAIVQQDGSNEV
                     AFVNLTLDGDQKVGNFTLSFTDLNVPVSGIPMQVVRTYDSRRAALGPLTGGAGSGGAS
                     GAGLPGGDFGAGWTLDIHNIQVEESGVMGEGWDTYVQSGGLLQGSFGQIFIDPVESHF
                     VDIVMGNGKVYRFEPHFFDAQNHDVAPGGHGEQDISGGTFGVVFEPLSGTAPNCSLVP
                     LDGNGQAVTDIYANGSPDVVPDDLTWTTDGGGDYNPTRFRFTDEAGNQYTVNTTAGLE
                     QMKDLNGDTLKVDAYGIHWTGNAQGTKDIL"
BASE COUNT          364 a          766 c          667 g          339 t
ORIGIN      
        1 ctggcccccg ggcaataccg ggtgacggtc aagatggtga acttgaccta tttgcaaccc
       61 agccaggatg tgttcaacat cttcgccaac gggaccgaag tggtgcccaa cttggacctc
      121 ttcgagtccg cggattacgg ccaagccttc gacgtcacct ttacggtcca ggtggacgct
      181 tccgccgatt ggctcgagtc gaacgcgggg gtggggacgc tggacctgtt ggcttccgcc
      241 tccaagggga ccgcgaccgt ctcggccatc gaggtggtgg gcctgcaggc ccagccgccc
      301 cccctgccga ccaggacccc cgtcccgggg gccttggact tccgcctcaa cgcggcgggg
      361 cccgccgtca cggattccat gggggcgctt tggaccgccg atcagttctt cggctcggcc
      421 acgggtgttc cgggactggg ggctttcggc tggacccaag gcgtgaccga agtgaccgtt
      481 cccgatggtt tcgtgggcaa ccacagcacc ccggacccgg ccctttatga gacctaccgc
      541 gaggaccgga cggtgggcta ccgttttgcc gtgcccaacg ggctttacca ggtgaccttc
      601 aagtgggccg agttggaata tgaccttccg ggacagagga tcttcaacgt tctggcccaa
      661 gggcagaccc tggccccgag ggtcgacctg gacaccaccg tcaattttgg ggacgctttc
      721 gaccagacct tctacgacat ccccgtcacc aacggcgtcc tggatgtgag cttccaggcc
      781 ctgccggggt ccttgggcgg gggcatggtc tcggcgatcc atgtggcggg ggagcaaccc
      841 atcccttcgg ccacgcccac cggcaccccc accaacagtc ccacggtgac caacagcccc
      901 acggccaccg gcacccccac ggatacagcg acattgaccc ccacccccgt gggcacctgg
      961 tacacggcca cccccacctc caccgccacg gccactccca cccccctgcc gccggacgcc
     1021 gaattgctta agctccaaga gctgcccacc ctgccccacc ccaaccccac ggcggtggac
     1081 gccgaggggg cccgggtcac ggccccggtg agcgtcatcg gcacggccaa cggcaatttc
     1141 gcgcccgccc cgcccaaccg gggttaccag agcggttggt tcctggaact gcagcccctg
     1201 tccccggcgg gcgcctcggc ggtcaccctg gcctcgggca cggcccaggt caccaacggc
     1261 cccctgggcg tgctggaccc gacccttttg ctcaacgggg cctatcagtt ggaactggcc
     1321 atcgtccaac aggacggcag caatgaggtg gccttcgtga accttacgtt ggacggcgac
     1381 cagaaggtgg gcaacttcac cctgtccttc accgacctca acgtgccggt ctcggggatc
     1441 cccatgcagg tggtgcgcac ctacgacagc cgccgggcgg cccttggccc cttgaccggc
     1501 ggggcgggtt ccggcggtgc ttcgggggcg ggcttgcccg gcggagattt cggagccggg
     1561 tggacgctcg acatccacaa catccaggtg gaggaatcgg gggtcatggg cgagggctgg
     1621 gacacctacg tccaatcggg cggcctcttg caggggagct tcggccagat cttcatcgat
     1681 cccgtggaat cccacttcgt ggacatcgtg atgggcaacg gcaaggtcta ccgcttcgag
     1741 ccgcatttct tcgacgccca gaaccatgac gtggccccgg gcggccacgg cgaacaggac
     1801 atctcgggcg gcaccttcgg cgtggtcttc gagcccctgt ccggcacggc gcccaattgc
     1861 tccctggtgc ccctggacgg caacggccag gccgtgaccg acatctacgc caacggcagc
     1921 ccggacgtgg tgcccgacga cctgacctgg accaccgacg gcgggggcga ctacaacccc
     1981 acccgcttcc gtttcaccga cgaggccggc aaccaataca ccgtgaacac cacggcgggt
     2041 ctggaacaga tgaaggacct gaacggcgac accctgaagg tggacgccta cggcatccat
     2101 tggacgggca acgcccaggg gaccaaggac atcctc
//
LOCUS       DASTJJ010000829         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1093811, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000829 DASTJJ010000000
VERSION     DASTJJ010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093811"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFR02_10000"
     CDS             complement(<1..532)
                     /locus_tag="VFR02_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HET9870812.1"
                     /translation="MGAFYLLKRPRETPHNRKNIATQVLLEHVTRYRYGRHVRLGPQV
                     IRLKPAAHCRTPILSYSLSITPKKHFLNWQQDPFGNFQARAVFPEPTGEFEVKVGLRA
                     DLERINPFDFFIEGDYLEFPFRYEKTLAADLRPYLTPSESGPRLRKFVAGIGRKKRHT
                     IELLAQVNQAINRQSKY"
     gene            531..2312
                     /locus_tag="VFR02_10005"
     CDS             531..2312
                     /locus_tag="VFR02_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET9870813.1"
                     /translation="MSNFFSLSFDNVASPSVRLKAAGGDPALDWGLVWYAGDDGPASL
                     VRGSGDGTEDLKRALADWSKFRSASFFALSGERQSGKNTPPFLRSYGGRQFVFVFNAS
                     LKGDFKSALSLGGDPSFEPLGSSAAEHAFCWLLHQLFLKQARRLSDVPWGELRSWFQQ
                     VNELGELNCVLSDGKNQVAYRDREGRGGLYGLRRLPPHPLGHLESEALTLDLGDSMDA
                     NRTLYLFSSQPLSGEPWTLLEPGSLRAVRRGRLLYDSSIADVALTRSWSLVGSQGQQT
                     SLLPPPVLGAAAPPRKAYDRCVMNVLHETTYHYRMPVERSRHVLRLRPVQDRFQELLE
                     FNLYLSVDGQKREFQDVFGNATLELDVDKPFTEMVIRASSRVRLSEGPTRWLNTGGKR
                     DTLPTVWMPGQRQMMLPYLLPPELPEAQLKELTEYAMGFAQRNDMDLLDTILDINQTL
                     HDDYAYVSGSTTSETTPYEIYSTRRGVCQDFSNLFICLARLLNLPARYRVGYIYTGGN
                     YENKIWSEASHAWVELFLPQGGWCGFDPTNGSVVGLDHVRVACGRHYLDATPTSGTLF
                     KGGGPEALSVSVKIEMEPPSQLSFDNG"
BASE COUNT          446 a          904 c          888 g          515 t
ORIGIN      
        1 ggtacttgct ctggcggttg atggcctggt tgacctgggc cagcagctcg atggtgtggc
       61 gtttcttgcg gccgatcccg gcgacgaact tgcgcagccg cgggcccgac tcggacggcg
      121 tcaggtaggg cctgaggtcc gcggccaggg tcttttcgta gcggaagggg aattccaggt
      181 aatcgccctc gatgaagaag tcgaaggggt tgatgcgctc caggtcggcc cgcaggccca
      241 ccttgacctc gaactcaccc gtgggctcgg ggaagacggc ccgcgcctgg aaattgccga
      301 aggggtcctg ctgccagttc aggaaatgtt tcttgggcgt gatggagagc gagtaggaca
      361 ggatgggggt gcggcaatgg gcggcgggct tgaggcggat gacctggggc cccaggcgca
      421 catggcggcc gtagcggtac cgggtgacgt gctccaggag gacttgggtg gcaatatttt
      481 ttcgattatg gggggtttcc cgggggcgtt tcaataggta gaatgccccc atgtccaact
      541 tcttctcatt gtccttcgac aatgtggcct cgccttcggt gaggctgaag gccgccggag
      601 gggaccctgc cctggattgg ggcctggtct ggtacgccgg cgacgacggg cccgccagcc
      661 tggtgcgggg gtccggcgac gggaccgagg acctgaaacg cgccctggcc gactggagca
      721 agttccgctc cgcctccttc ttcgccctgt ccggcgagcg ccaatccggc aagaacaccc
      781 cccctttcct gcgcagctac ggcggccgcc agttcgtctt cgtcttcaac gcgtccctca
      841 agggcgactt caagtcggcc ctgtccctgg gcggcgaccc cagtttcgag cccctgggct
      901 cgtccgcggc cgaacacgcc ttctgctggc tgctgcacca gcttttcctc aaacaggccc
      961 ggcggctgtc cgacgtgccc tggggggaac tgcggtcctg gttccagcag gtcaacgagt
     1021 tgggggagct gaactgcgtc ttgagcgacg gcaagaacca ggtggcctac cgggaccgcg
     1081 agggccgggg cgggctgtat ggcctgaggc gcctaccccc ccatcccctg ggccacctgg
     1141 aaagcgaggc cctgaccctg gacctgggcg acagcatgga cgccaaccgc acgctgtacc
     1201 ttttttccag ccaacccctg tcgggggaac cctggaccct gttggagccc ggatcgctgc
     1261 gggccgtgcg ccgggggcgc ctgctctacg acagttccat cgccgacgtg gccctgaccc
     1321 gttcctggtc cctggtgggg agccagggcc agcagacgtc cctgctgccg ccgcccgtcc
     1381 tgggggcggc cgcccccccg cgcaaggcct atgaccgctg cgtgatgaac gtgctgcatg
     1441 agaccaccta ccattaccgc atgccggtgg aacgctcgcg ccatgtgctg cgcctgcggc
     1501 ccgtgcagga caggttccag gaactgctgg agttcaacct ctacctctcg gtggacggcc
     1561 agaaacgcga gttccaggac gtgttcggca acgccaccct ggagctggac gtggacaaac
     1621 ccttcaccga aatggtgatc cgcgcctcgt cccgggtgcg cttgagcgag ggccccaccc
     1681 gctggctcaa caccgggggc aagcgggaca ccctgcccac ggtctggatg ccgggccaac
     1741 ggcagatgat gctgccctac ctgctgccgc ccgagctgcc cgaggcccag ttgaaggagc
     1801 tcaccgaata cgccatgggc ttcgcccagc gcaacgacat ggacctcttg gacaccatcc
     1861 tggacatcaa ccagaccctg cacgacgact acgcctacgt gagcgggtcc accaccagcg
     1921 agaccacgcc ctacgagatc tattcgaccc ggcgcggcgt ctgccaggac ttttccaacc
     1981 tcttcatctg cctggccagg ctcctcaacc tccccgcccg ctaccgggtg ggctacatct
     2041 atacgggcgg caactacgag aacaagatct ggtccgaggc ctcccacgcc tgggtggagt
     2101 tgttcctgcc ccagggcggc tggtgcggtt tcgaccccac caacggcagc gtggtggggc
     2161 tggaccacgt gcgggtggcc tgcggccgcc attacctgga cgccaccccc acctcgggca
     2221 cgctcttcaa gggcggcggc cccgaggccc tgtcggtctc ggtgaagatc gagatggagc
     2281 cgccctcgca gctttccttc gacaacggct gaagcccaaa acggaaaagg cctttcacgg
     2341 agacggcgga gtggaaggcg gagttttagg ccccgttccc ttccacgaaa agccatcatc
     2401 catcaccgtt ctgtttgtct ttggccattg ctttcacttg gtgtctttgc gactttgcgg
     2461 taaaagggat ttcgtgacgg tctcttcgga actcggtttt tcactcggtg tcctccgtgg
     2521 aaagccttga tttgtcttgg ttccgggccc cggcttgaca agcgggggac cggcgggtag
     2581 aatccttcca ctgcagttca tgcttaggag acagaagatg accttcggaa cccagaccca
     2641 gcttttaacc gtagccgtag tcgttggtct gcgtccggag gcgttgcgcc cctaagagac
     2701 ggccaccagg ccgcggggtc ccgccaccgg gaccgccgcg gcttttttat ttt
//
LOCUS       DASTJJ010000830         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_847528, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000830 DASTJJ010000000
VERSION     DASTJJ010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847528"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..481)
                     /gene="queF"
                     /locus_tag="VFR02_10010"
     CDS             complement(68..481)
                     /gene="queF"
                     /locus_tag="VFR02_10010"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET9870814.1"
                     /translation="MKKDKTSVKDYNEAHAKSGISVKLPSVETFPNQYPGYTIKIEIP
                     EYTAVCPKTGLPDFGTIILEYEPVKHVLELKSLKGYILAYRNVGIFYENAVNRMLRDF
                     SAAARPKWCRVTGVFATRGGMHSTVTAVFGKPPRA"
     gene            complement(627..1010)
                     /locus_tag="VFR02_10015"
     CDS             complement(627..1010)
                     /locus_tag="VFR02_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9870815.1"
                     /translation="MEIRGMDLVAFHVGDLNAGTRYYEDLGLTVQFRSNELGVTVFLV
                     GGEFPKLLVTTEPPPAPPGTSRMWVEVADVQAILPELKAKTAAIVREPLLIATGWLVE
                     TADPWGNVLGFRDYTAQPDYGRPKK"
     gene            complement(1032..2105)
                     /gene="mqnC"
                     /locus_tag="VFR02_10020"
     CDS             complement(1032..2105)
                     /gene="mqnC"
                     /locus_tag="VFR02_10020"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HET9870816.1"
                     /translation="MSVAAAQTLQEKILDGHVVDRAEALALYQGSELNDLGAVADALK
                     VRSRGDKIATYLIDRNINYTNVCVTVCKFCAFYREPGNAEGYVHEADKIVHKITEAKQ
                     KGCTQILLQGGHHPDLTLDWYLNMLSKIKGAHPDVTLHAFSPSELVHFAETFRMPVKE
                     ILVKFREAGMDSMPGGGAEILVDRVRQEIAPLKANTEEWLGVMRELHGLGMKSTATMM
                     FGHVETVEERIEHIFRIRELQEETGGFLGFIPWLYQPGNESLGLKSASGMEYLKTLAL
                     ARVILNHCLPNLQASWVTPGKKIGQLGLKYGANDLGSIMLEENVVASTGLRYLMSVEE
                     MRRLITEMGYEPHQRDTFYNLVN"
     gene            complement(2102..>2611)
                     /locus_tag="VFR02_10025"
     CDS             complement(2102..>2611)
                     /locus_tag="VFR02_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET9870817.1"
                     /translation="PQGIRRLLVDDRSQTSVLLSRLLLKRWFGVKDLEVEPVTMASFH
                     PGRTKPWEAVLQFGDNALLSAPTGMTVLDLGEEWTRFTGKPFVYAAWMVKDASLAGEL
                     QPQLEEAKAGGLANLEPITAGYHGIWQFKRADAKTYLEKNIDYAYGPEQREGHAEFQK
                     LLKEEGLIP"
BASE COUNT          418 a          812 c          858 g          523 t
ORIGIN      
        1 ttgtgaattc cgggaaaaga tggggccccg ggaaaaggcc taacccggcc tttttcgggg
       61 ggaggggtca ggcccggggg ggcttgccga agaccgccgt gacggtggaa tgcatgccgc
      121 cccgggtggc gaagaccccg gtcacccggc accacttggg gcgggcggcg gcggaaaagt
      181 cccgcagcat gcggttgacc gcgttctcat agaagatgcc cacgttgcgg taggccagga
      241 tgtagccctt gagggacttg agctccagca cgtgcttcac gggctcatat tccaggatga
      301 tggtgccgaa gtcgggcagg ccggtcttgg ggcagaccgc cgtgtactcg gggatctcga
      361 tcttgatggt gtagcccggg tactggttgg ggaaggtctc cacggagggc agcttcaccg
      421 agatccccga cttggcatgg gcttcgttgt agtctttcac gctggtcttg tcctttttca
      481 tcttcaatat ctttcacttt taaagatcaa atccatttca acaatgatgc aacctgatga
      541 aggctgatgt cggatcccaa agatggaaca tcagtattta tcagccttcg tcattgtaaa
      601 tacgttcttc gctcgttccg ccctttttac tttttcggcc ggccgtaatc gggctgggcg
      661 gtatagtccc ggaaccccag cacgttgccc caagggtcgg cggtctccac caaccagccc
      721 gtggcgatga gcagcggttc ccggacgatc gcggcggtct tggccttgag ttcgggcagg
      781 atggcctgca cgtccgccac ctccacccac atccgggagg tccccggcgg ggcgggcggg
      841 ggctcggtgg tcaccaacag cttggggaac tcccccccga ccaggaagac cgtgacgccc
      901 aactcgttgg agcggaactg gaccgtcaag cccaggtcct cgtaataacg ggtccccgcg
      961 ttgaggtccc ccacgtggaa ggcgaccagg tccatccctc ggatttccac ggcgccgcct
     1021 tttcccggtg ctcagttcac caggttatag aaggtgtccc gctggtgcgg ctcatagccc
     1081 atctcggtga tgagccggcg catctcctcg acgctcatca ggtaccggag gcccgtggag
     1141 gccaccacgt tctcctccag catgatgctg cccaggtcgt tggcgccgta cttgaggccc
     1201 aactggccga tcttcttgcc cggggtgacc caggaagcct gcaggttggg caggcagtgg
     1261 ttcaggatga cccgggccag ggccagggtc ttgaggtatt ccatgccgct ggcgctcttg
     1321 agccccaggc tctcgttgcc cggctggtag agccagggga tgaagcccag gaacccgccg
     1381 gtctcctcct gcagttcgcg gatgcggaag atgtgctcga tgcgctcctc cacggtctcc
     1441 acgtggccga acatcatggt ggcggtggac ttcatgccca ggccgtgcag ttcccgcatg
     1501 acgcccagcc actcctcggt gttggccttg aggggggcga tctcctggcg gacccggtcc
     1561 accaggatct cggccccgcc gcccggcatg gagtccatgc ccgcctcgcg gaacttgacc
     1621 aggatctcct tgaccggcat gcggaaggtc tcggcgaagt gcaccaattc ggagggcgag
     1681 aaggcgtgca gggtcacgtc cggatgggcg cccttgatct tggagagcat gttcaggtac
     1741 cagtccaggg tcaggtccgg gtggtggccg ccctgcagca ggatctgggt gcagcccttt
     1801 tgcttggcct cggtgatctt gtggacgatc ttgtcggcct catgcacgta gccctcggcg
     1861 ttgcccggtt cccggtaaaa ggcgcagaac ttgcagaccg tcacgcagac gttggtgtag
     1921 ttgatgttgc ggtcgatcag gtaggtggcg atcttgtcgc cgcgggaccg caccttcagg
     1981 gcgtcggcca cggcccccag gtcgttgagt tcggacccct ggtaaagcgc cagggcctcc
     2041 gcgcggtcga ccacgtggcc gtccaggatc ttttcctgca gggtttgggc ggcggcgacg
     2101 ctcatgggat caggccctcc tccttcagga gcttctggaa ctcggcgtgg ccctcccgtt
     2161 gctcggggcc gtaggcgtag tcgatgttct tttccaggta ggtcttggcg tcggcgcgtt
     2221 tgaactgcca gatgccgtgg tagccggcgg tgatcggttc caggttggcc aggcccccgg
     2281 ccttggcctc ctccaactgg ggctgcaatt ccccggccag ggacgcgtcc ttcaccatcc
     2341 aggcggcgta cacgaagggc ttgcccgtga accgggtcca ttcctcaccc aggtccagca
     2401 cggtcatgcc cgtgggcgcg gacaacaggg cgttgtctcc gaactgcagc accgcttccc
     2461 agggcttggt ccggcccggg tggaaggagg ccatggtgac gggctccacc tccaggtcct
     2521 tcacgccgaa ccagcgcttg agcagcaggc gcgacaagag cacggaggtc tgggaccggt
     2581 cgtccaccag caggcggcgg atgccctggg g
//
LOCUS       DASTJJ010000831         1790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1583508, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000831 DASTJJ010000000
VERSION     DASTJJ010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583508"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFR02_10030"
     CDS             complement(<1..1346)
                     /locus_tag="VFR02_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HET9870818.1"
                     /translation="MKPAQGLLAARLLLISILLLSLPAGPAGAATYTVSNLAGDDTTT
                     NGLGYEMNQANGDGYNVNTTINFSSLFNTPQTITTFFTSPNIYKQPGNNLTLTPTVGL
                     LTINGFAGAPFVFQGGGTLSLGNMIIENNASLAIDGSTLALSNVTENGDMSLTNAVTV
                     NTQGTNDSIGGQLYGGGSLTKTGSGALTLSNDGNLQDDTIVRGGTLSVSSDGQMGYTF
                     GYLAVDGGTFQTTASFSSDRFIILGGGTFDVTSGTTLTLTGQLTNGGGLSLTDTGTLV
                     LTNNTNSYTGGTVVGSGSTLSISNDAQLGNSSGNLTLNGGTLETTAGFSSSRNLLLSG
                     GATLDTASGTTDTWNGLVSGTGGLNVTGPGTLVLADSSNSYTGGTAVTGGTLSISADG
                     DLGDSSGNLTLDGGTLQTTSTLSSSRGFSLLAGGGTLNVATATTTTWNGQVGGAGSL"
     gene            complement(1695..>1790)
                     /locus_tag="VFR02_10035"
     CDS             complement(1695..>1790)
                     /locus_tag="VFR02_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET9870819.1"
                     /translation="EYIEVFYNRTRMHSSLGYKSPVEFEKQVTLS"
BASE COUNT          333 a          500 c          592 g          365 t
ORIGIN      
        1 aggcttcccg cgccgccgac ctggccgttc caggtggtcg tggtcgcggt cgccacgttc
       61 aaggtgccgc cgcccgcgag caggctaaaa ccccggctcg aagagagggt ggaggtggtc
      121 tgcagggtgc ccccgtccag ggtgagattg cccgagctgt cgcccaggtc gccgtcggcg
      181 gaaatggaca gagtcccccc cgtcacggcc gtcccgcccg tatagctgtt cgagctgtcg
      241 gccaggacca gcgtgccagg gcccgtgacg ttcaagcccc ccgtcccgga aaccagcccg
      301 ttccaggtgt ccgtggtgcc ggaggccgtg tccagcgtgg ccccaccgga caacaggagg
      361 ttgcgcgagg aggagaagcc ggcggtggtc tcaagcgtgc cgccgttcaa ggtgaggttg
      421 cccgagctgt tgcccagctg ggcgtcgttg ctgatgctca aggtgcttcc cgaccccacc
      481 acggtgccgc ccgtgtagct gttcgtgttg ttggtcagca ccaaggtgcc cgtatcggtc
      541 aagctcaaac cgccgccgtt ggtgagctgg cccgtcaggg tcagggtggt gccggaggtc
      601 acgtcgaagg tgccgcctcc caggatgatg aaacggtccg aggagaaact ggcggtggtt
      661 tggaaggtgc cgccgtccac ggccaggtaa ccgaaggtat agcccatctg gccgtccgag
      721 ctgacgctta aggttccgcc ccggacgatg gtgtcgtcct ggaggttccc gtcgttcgac
      781 aaggtcagcg cgcccgagcc cgtcttggtc agggagcccc cgccgtagag ctgtcccccg
      841 atgctgtcgt tcgtcccctg ggtgttcacc gtcacggcgt tcgtcaaact catatcgccg
      901 ttctccgtca cgttggaaag cgccagggtg ctgccgtcaa tggccaggga ggcgttgttt
      961 tcgatgatca tgttgcccag cgaaagggtg ccgcctcctt ggaagacgaa gggggcgccg
     1021 gcgaagccat taatcgtcaa caaacccaca gtgggggtca gggtcaggtt gttgcccggc
     1081 tgtttgtaga tgttggggct ggtgaagaag gtcgtgatcg tctggggggt attgaagagc
     1141 gagctgaagt tgatggtggt gttgacgttg tacccatccc cattggcctg gttcatttca
     1201 tatcccaaac cgttggtggt cgtgtcatcc cccgcgaggt tggacacggt ataagtggcc
     1261 gccccggcgg gtcccgcggg gagggagagg aggagtatgg aaataagaag aaggcgcgcg
     1321 gccaaaagac cttgggcggg tttcacgggt gactccttgg atcgaggttt ggcatcgggt
     1381 caagggaccg ggtcgtcatg aaaaggtgtg gaatggtcag aaattttaac ccaagagccc
     1441 aggccccgcc acaagggaat ggcccattga aaagggattt ttcggggatt ttcgatgaaa
     1501 gggttttctt caaagggacg tgaaaaatca aatagaacca ggctgacaat gaccgtttaa
     1561 agtttcccgg ctcttcgggt ggcggtcgct ttcggacctg tccgcgcccc tcgaaaagtg
     1621 tgactgacct gcccccgttg tttgagtaaa agacataggg gtcaggctgg acttccccca
     1681 gaaaaatgga caactcagct tagggtaact tgcttctcga actcaaccgg acttttgtac
     1741 cccaggctcg agtgcatcct ggtccggttg tagaaaacct cgatatattc
//
LOCUS       DASTJJ010000832         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1704586, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000832 DASTJJ010000000
VERSION     DASTJJ010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704586"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..484)
                     /locus_tag="VFR02_10040"
     CDS             complement(20..484)
                     /locus_tag="VFR02_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET9870820.1"
                     /translation="MRKTIRLLGLSAALLAAAATACHPKDRSGLPQVTLTIDGHPVEA
                     EVADQEATRMTGLMFRREMDPDHGMVFVFPDSQIRAFWMKNTFIPLSIAYMDDRGVIL
                     NILEMPPQTEDAFYSDGLAKYALEMNSGWYKAHGVKPGDRVEGLAAAPPAAP"
     gene            591..1607
                     /locus_tag="VFR02_10045"
     CDS             591..1607
                     /locus_tag="VFR02_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870821.1"
                     /translation="MRKNLRFLPLCAFLLAPSLSTAAPSIEAAYQYGYSLFTANNFKE
                     SKQYYLYVVLNGQDAELKANSLYMLCQCYLRLGDYNNCVKAANMLAGGYPRSTVVKNG
                     MLTTFATSLISQISNLQTDWDYWRYQDGTDDKDQPIWKESVPVGKPIKRIDFRLAFGL
                     YRALEKINPASPQVAAAKKQLDGMLNRPLTIIWVDQKAAPNDQGHPADFFSLFTKKEK
                     KKFSQVICERMFYDWKTDMLYRFMDMYDDVRNHNPQYTAKTFNLDEDNSSTSPLVAPA
                     GSAAPPPPPGAPAPSTATVSANDSFNEPVLTLAKLFAAAAYNPYSDTFGNPAEISGAV
                     APSL"
     gene            1614..1961
                     /locus_tag="VFR02_10050"
     CDS             1614..1961
                     /locus_tag="VFR02_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870822.1"
                     /translation="MRSLPTRRPASLLPLLWAAFAALPLNAATPTATPSTPVTAAGGG
                     ARLLEIHKPLIPAAWGRVLQYRKETNISAGGSQETLYEFVLQTEDGVVRTATYHESTD
                     GTGYWEVYVWDEP"
     gene            1958..>2470
                     /locus_tag="VFR02_10055"
     CDS             1958..>2470
                     /locus_tag="VFR02_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870823.1"
                     /translation="MNPRAKVLAFWGAQPRVGTSTLALWSARLQASPQPLSCALVSAG
                     PLRGASPTPSPDARTFQQLAPYAGHLTRSTLEHFFDRSPEGLHLIPLDAPGRPPAGED
                     AFLRALEALRPFFELLIVDLPAPAGGPLPAALLEACGEVVVVASFEDSSLQALQRMEK
                     VLLARHLSLKP"
BASE COUNT          432 a          904 c          680 g          454 t
ORIGIN      
        1 ccgagggggc gctgccttct tagggcgccg ccgggggggc ggcggccaat ccctccaccc
       61 ggtcgcccgg cttgacgccg tgggccttgt accatccgct gttcatttcc agggcgtact
      121 tggccaggcc gtccgagtaa aaggcgtcct cggtctgggg gggcatttcc aggatgttca
      181 ggatcacccc ccggtcgtcc atgtaggcga tgctcagggg gatgaaggtg ttcttcatcc
      241 aaaaggcccg gatctgggaa tccgggaaca cgaagaccat gccatggtcc gggtccatct
      301 cccggcggaa catcaatccc gtcatgcggg tggcctcttg atcggccact tccgcctcca
      361 ccgggtgccc gtcgatggtc aaggtcacct gcggaaggcc ggagcggtcc ttgggatgac
      421 aggccgtggc cgcggccgcc aacaaggcgg ctgaaagtcc tagaaggcgg atggtttttc
      481 gcatggcttc cttttccggg gcgggcaagg ttcaaaaacc cgtattttta aaaggttttc
      541 caagatatag tcgttgacgg tccaaatgca attgtcttaa gataatcttt atgagaaaaa
      601 atctccggtt tctcccgctt tgcgcctttc tgctggcgcc ttccctttcg acggccgcgc
      661 cctccatcga ggccgcctac caatacggct acagcctctt caccgcgaac aatttcaagg
      721 aatccaagca atattacctc tatgtggtgc tgaacgggca ggacgcggaa ctcaaggcca
      781 attccctcta tatgctctgc caatgttacc tgcggctggg cgactacaac aattgcgtaa
      841 aggccgccaa catgctggcc gggggctatc cccgctccac ggtggtcaag aacggcatgc
      901 tgaccacctt cgccaccagc ctcatcagcc agatctccaa cctgcagacc gattgggact
      961 actggcgcta ccaggacggc acggacgaca aggaccagcc catctggaag gaaagcgtgc
     1021 ccgtgggcaa acccatcaag cgcatcgatt tccggctggc cttcggcctc taccgggccc
     1081 tggaaaagat caaccccgcc tcgccccaag tggccgccgc caagaaacag ttggacggca
     1141 tgttgaaccg ccccctcacc atcatttggg tcgaccagaa ggcggccccc aacgaccagg
     1201 gccacccggc ggacttcttc tcccttttca ccaagaaaga gaagaagaaa ttctcgcagg
     1261 tcatctgcga gcgcatgttc tacgactgga agaccgacat gctctaccgc ttcatggaca
     1321 tgtacgacga cgtgcggaac cacaaccccc aatacaccgc caagaccttc aatttggacg
     1381 aggacaattc ctccacgtcc cccttggtcg cgccggccgg ctcggccgcg ccgccccccc
     1441 cgcccggcgc cccggcgccc tccacggcca ccgtcagcgc caacgattcc ttcaacgaac
     1501 ccgtcctgac cctggccaag cttttcgccg ccgccgccta caatccctat tcggacacct
     1561 tcggcaatcc cgctgagatc tcgggggccg tggcccccag cctgtaaccg gccatgcgga
     1621 gcctccccac ccggcggccg gcttccctgc tgcccctcct gtgggcggcc ttcgccgcgc
     1681 tccccttgaa cgccgccacc cccacggcca ccccttccac tcccgtcacc gcggccggcg
     1741 gcggcgcgcg cttgctggag atccacaaac ccctgatccc cgcggcctgg ggccgggtcc
     1801 tgcaataccg gaaagagacc aatatttccg ccggcggaag ccaggaaacg ctgtacgaat
     1861 tcgtccttca aaccgaggac ggggtcgtcc gcacggcgac ctaccatgaa tccaccgatg
     1921 gcaccggcta ttgggaagtg tacgtctggg acgagccgtg aacccccggg ccaaagtcct
     1981 ggccttttgg ggcgcccagc cccgggtcgg aacctccacc ctggccctct ggtcggcgcg
     2041 cctccaggcg tccccccagc ccctctcctg cgccctcgtg agcgcgggtc cgctccgcgg
     2101 ggcctccccg accccctcgc ccgacgcccg caccttccaa caactggcgc cctacgcggg
     2161 gcacctcacc cgttccacct tggagcattt tttcgaccgt tcccccgagg ggctccacct
     2221 cattccgttg gacgcccccg gccggccgcc cgcgggggaa gacgcctttc tccgggccct
     2281 ggaggccctg cgcccttttt tcgaactgct catcgtcgac cttcccgccc cggcgggcgg
     2341 tccccttccc gccgccctct tggaggcttg cggggaggtc gtggtcgtcg cctccttcga
     2401 ggattcttcc ctgcaggccc tccagcgcat ggaaaaggtc ctgcttgccc gccacttgtc
     2461 cttgaagccc
//
LOCUS       DASTJJ010000833         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1704601, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000833 DASTJJ010000000
VERSION     DASTJJ010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704601"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFR02_10060"
     CDS             <1..440
                     /locus_tag="VFR02_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017710969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HET9870824.1"
                     /translation="GVRAYVGFRQTGVDYVRPERMFGRSTNNLFKNIEWAKKGILSFS
                     YTPLSVLSTCGILLFLFTVLLGFAQLALRLLYPDLAPKGITTVLLAILFLGSVNLFSL
                     AVVGEYIAKIFEEVKRRPHFVRRALIKDGEVRDAAAPDRSLSE"
     gene            437..>1108
                     /locus_tag="VFR02_10065"
     CDS             437..>1108
                     /locus_tag="VFR02_10065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870825.1"
                     /translation="MTVALKSRSCPVCGTHDEARVFAPENFDLGTLDDYAFASRKTPE
                     YMHFRLLECPGCGLLYADPILPPAKIAALYRKAAFASQEEAAAAAATYGRVLDGFLAD
                     LPDRKGALDIGTGEGAFLGELTGRGFTGVVGVEPSKAPVQAAKPALRKLIRNKPFRAG
                     DYRKNSFSLVTCFQTLEHLVEPLALAKAAHSLLKPGGVFFVVSHDRQSLPMRLLGRKS
                     PIFDIE"
BASE COUNT          173 a          382 c          350 g          203 t
ORIGIN      
        1 ggggggtgcg ggcctatgtg ggcttccgcc agaccggggt ggactacgtc cggcccgagc
       61 gcatgttcgg ccgcagcacc aacaacctct tcaagaacat cgagtgggcc aagaagggca
      121 tcctgtcctt cagctacacg cccttgagcg tgctgagcac ctgcggcatc ctgctgttcc
      181 ttttcaccgt gctgctgggt ttcgcccagt tggccctgcg gctgctttat cccgacctgg
      241 cgcccaaggg catcaccacg gtcctgttgg ccatcctgtt cctgggctcg gtgaacctct
      301 tttccttggc ggtggtgggg gaatatatcg ccaagatctt cgaggaggtg aaacggcgcc
      361 cccatttcgt gcgccgggcc ctgatcaagg acggcgaggt ccgcgacgcg gcggccccgg
      421 accggagcct gtccgagtga ccgtggcatt gaagtcccgg tcctgcccgg tctgcgggac
      481 gcacgatgag gcccgggtct tcgcccctga aaatttcgac ctgggcacgc tggacgacta
      541 tgccttcgcc tcgcgcaaga cccccgaata catgcatttc cgcctgctgg aatgccctgg
      601 ttgcggcctg ctctacgccg accccatcct gcccccggcc aagatcgccg ccctttaccg
      661 gaaagccgcc ttcgccagcc aggaggaggc cgccgcggcc gccgccacct atggccgggt
      721 gctggacggc ttcctggcgg acctgccgga ccgaaaaggg gcgctcgaca tcggcacggg
      781 ggaaggcgcc ttcttggggg aactgacggg ccggggtttc accggtgtcg tgggggtgga
      841 accctcgaaa gcccccgtgc aggcggccaa gccggcactc cgaaagctca tccgcaacaa
      901 acccttccgg gcgggggact accgcaagaa cagcttcagc ctggtcacct gcttccagac
      961 cctggaacac ctggtggagc ccctggccct ggccaaggcg gcccattcgt tgctgaaacc
     1021 cggcggcgtc tttttcgtgg tgtcccacga ccgccagtcc ctgcccatgc ggcttttggg
     1081 gcgcaagtcg cccatcttcg acatcgag
//
LOCUS       DASTJJ010000834         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_603074, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000834 DASTJJ010000000
VERSION     DASTJJ010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603074"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1261)
                     /gene="hisC"
                     /locus_tag="VFR02_10070"
     CDS             complement(173..1261)
                     /gene="hisC"
                     /locus_tag="VFR02_10070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9870826.1"
                     /translation="MAFPVPLRAHLKNLAAYVPGKPVEEVERELGITGAVKLASNENP
                     LGPSPRVAEALRAAIDQVQRYPDGSVFYLKRALAARLGVGPERLMVGHGSNELLVLLA
                     QVVLEPGDEVLYADPSFVVYPIAAKLFQAVGRPVPLKDHTHDLPAFTAALGPRTRLVY
                     LCNPNNPTGTVVPLLAVEAFLKTCGPDVLVVLDEAYYEYVEMKTYFESVRLLEKYPNL
                     VILRTFSKAYGLAGLRVGYGLGDPALVDAFQRVRQPFNVNSLALAGAEAALGDGDHVR
                     KVLDLNRASRAAIEGGLDRLGLAHPPSHANFVYFQAADAPALYGSLLAQGVITRPMGP
                     RALRVTTGTMEETERFLSTLEKTMGAKP"
     gene            complement(1266..>1813)
                     /locus_tag="VFR02_10075"
     CDS             complement(1266..>1813)
                     /locus_tag="VFR02_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET9870827.1"
                     /translation="SGDAGKVRVVYSHPQALAQTRLWLETHLPDVKTVEVANTAKAAQ
                     MAARNPRAAAVAADLAARLYGLKAIARRIEDLSDNQTRFLIIGRAQAEPTGRDRTSIM
                     VSIKDKVGALYSLLTPFEKRGISLTNIESRPSRKRAWDYYFFIDILGHVRDPKVRKAL
                     EEIERSALTVKVLGSYPRGEF"
BASE COUNT          290 a          611 c          621 g          291 t
ORIGIN      
        1 caggctgggc gccatgggca gcagggcccg ggcccggcgc cgcgaagcgg cccggtggtt
       61 ccagcccgcc aattcatagc ccccccgctt ttggagggcc agggccaagg atccgcccaa
      121 gaggcccacg ccgatgatgg cgatcttttt tctcttccgc gcgcggttca tgtcagggct
      181 tggcccccat ggtcttttcc agggtggaga ggaaccgctc ggtctcctcc atcgtccccg
      241 tggtcacccg cagggccctc gggcccatgg gccgggtgat gacgccctgg gccagcaggg
      301 atccgtaaag cgccggcgcg tccgccgcct ggaagtaaac gaagttggcg tggctgggcg
      361 gatgggccag ccccaaacgg tccagtcccc cctcgatggc ggcgcgggaa gcccggttca
      421 ggtccagcac cttccggaca tggtccccat cccccagggc ggcctcggcc ccggccaggg
      481 ccaggctgtt cacgttgaag ggttggcgca cccgctggaa ggcgtccacc agcgccggat
      541 cgcccaggcc atagcccacc cgcaggcccg ccaggccgta ggccttggaa aaggtccgca
      601 ggatgaccag gttcgggtat ttttccaaca accggaccga ctcgaaatag gtcttcatct
      661 ccacgtactc gtaataggcc tcgtccaaca ccaccaggac gtcggggccg caggtcttta
      721 agaaagcctc caccgccagc agcggcacca ccgtgcccgt ggggttgttc gggttgcaga
      781 gatagaccag ccgggtgcgg ggccccaggg ccgcggtgaa ggccggcagg tcgtgggtat
      841 ggtccttgag cgggaccggc ctccccaccg cctggaaaag tttcgccgcg atgggataga
      901 cgacgaagct ggggtcggcg tacagcacct catcgcccgg ttccaggacc acctgggcca
      961 gcagcaccag cagctcgttg gatccgtggc ccaccatcaa ccgctcgggg ccgaccccca
     1021 ggcgcgcggc cagggcccgt ttcaggtaga agaccgaacc gtccgggtaa cgctgcacct
     1081 ggtcgatggc ggcgcgcagg gcctccgcca cccggggcga gggacccagc gggttctcgt
     1141 tggacgccag tttgacggcc cccgtgatgc ccagctcccg ttccacttcc tcgacgggtt
     1201 tgccgggaac ataggcggcc aagttcttca ggtgggcacg gagggggacc gggaaagcca
     1261 tgggctcaga actcaccccg gggataggac cccaatacct tcaccgtgag ggccgaccgc
     1321 tcgatctctt ccagggcctt gcggaccttc gggtcccgga catgccccag gatgtcgatg
     1381 aagaagtaat agtcccaggc ccgcttgcgg gaaggccggg actcgatgtt ggtcaggctg
     1441 atgccccgct tctcgaaggg ggtcagcagc gaatagaggg cgcccacctt gtccttgatg
     1501 gacaccatga tggaggtccg gtcccggccc gtgggctcgg cctgggcgcg gccgatgatg
     1561 aggaagcggg tctggttgtc gctcaggtcc tcgatgcgcc gggcgatggc cttcaatccg
     1621 tagagccggg cggccaggtc ggcggcgacc gcggcggccc ggggattgcg cgcggccatc
     1681 tgggccgctt tggcggtgtt ggccacttcc acggtcttca catcgggcag gtgggtctcc
     1741 agccacaggc gggtctgggc cagtgcctgg ggatggctgt agaccacccg caccttcccc
     1801 gcgtcgcccg aga
//
LOCUS       DASTJJ010000835         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1704831, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000835 DASTJJ010000000
VERSION     DASTJJ010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704831"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFR02_10080"
     CDS             <1..482
                     /locus_tag="VFR02_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870828.1"
                     /translation="SVNLKTVFTLFQESGLDFIVPARRQHVMDRVQRLRDLLKPDPLK
                     KTHPMTQEYREEGWPTLLFTDECLDTIEEFENWELRGETSSEATVEKPEARNDHGIDA
                     VSYALQGFWGEASPDHPLTRMMEAKDPSRKLREVLLNDLKKSLTPTPAANYTGDQVL"
     gene            640..840
                     /locus_tag="VFR02_10085"
     CDS             640..840
                     /locus_tag="VFR02_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870829.1"
                     /translation="MTKHKFHPVKRPGALTKKADAAGESVQEFASQHYNDSGLTGQQA
                     RFAKIAKSWHHGKVKAKPHHFK"
     gene            851..1444
                     /locus_tag="VFR02_10090"
     CDS             851..1444
                     /locus_tag="VFR02_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870830.1"
                     /translation="MALTKEIGSKELWHHFKPSEMEMIAACAYGTWLKMPAMNEEKLR
                     TTPTNWTPYEMVMLAIEKLDLYFKSHAHEGLDWKIATVEPGFRRVSSGNFQLTNEENA
                     KSREVWEALFGKKGHGTDWGDAKYLQSLVDGAPGRLLKSFFGDPMSRPRFASFERTNE
                     YISSQSPLEDLLKKQGGKKPEPAGNPEDPKPSGSKAT"
     gene            1441..1665
                     /locus_tag="VFR02_10095"
     CDS             1441..1665
                     /locus_tag="VFR02_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870831.1"
                     /translation="MIYAVVPLAFLAAFLGAYLGATFALRRSPKPEPVDLAKWVEADK
                     RALASKDTTESRHMDEWAEQQVFMASQGGL"
     gene            1767..>2177
                     /locus_tag="VFR02_10100"
     CDS             1767..>2177
                     /locus_tag="VFR02_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870832.1"
                     /translation="MSLYENDKKARLIFDRYIYESTALYVGKHNLIWKDGAYTPMAAD
                     FLGQSQVVVNKIIGKCSAVVNRMTDFDPTAQALPASGSVSDTYAARAAKKILQSNHYS
                     YDFQRVYRRHALDIVVQGDGFLKTMYDPYGGRKSV"
BASE COUNT          495 a          710 c          597 g          375 t
ORIGIN      
        1 aaagcgtcaa tctcaagacg gtattcaccc tgttccagga atccggcctc gattttatcg
       61 tcccggccag acgccagcac gtcatggacc gcgtccaacg gctacgcgac ctcctgaaac
      121 cggaccccct caaaaagacc catcccatga cccaggagta tcgggaggaa ggatggccta
      181 ccctgctttt taccgatgag tgcctggata ccatcgagga gttcgagaac tgggagctgc
      241 gcggggagac ttcctccgag gcgaccgtgg aaaagccgga ggcccggaat gaccacggta
      301 tcgacgctgt ttcctacgcc ttgcagggct tttgggggga agcttccccg gaccacccgc
      361 tgaccaggat gatggaggcc aaagacccat cccgaaagct ccgggaggtc ctcctgaatg
      421 acctcaagaa atcgttgacg ccgaccccag ccgcgaatta cactggcgac caagtgctat
      481 gaaaccgcgc caacacctga accaggacca attcccgggt cttcccctcc agaaaggggc
      541 gactctcccg acaggtacgc cggaagtcca aaaacccaag gtcaactcac cccaccgggt
      601 ccgcgccaag aagcggacct tcaccaggac accgatgaca tgaccaaaca caaattccat
      661 cccgtgaaac gccccggagc tttgaccaaa aaggccgatg ccgccgggga gtccgtccag
      721 gagttcgcct cgcagcatta caacgactcc ggcctcaccg gtcagcaggc gaggttcgcc
      781 aagatcgcga agtcgtggca ccacgggaag gtcaaggcaa agccgcacca tttcaagtaa
      841 ggagtaggtc atggcgctta cgaaagagat cgggtcgaag gaactctggc accacttcaa
      901 gcccagcgag atggaaatga tcgcggcctg cgcctatggc acttggctca agatgcccgc
      961 gatgaacgag gagaaactcc ggacgacccc caccaactgg accccctacg agatggtcat
     1021 gctggccatc gagaagctcg acctctactt caaatcccac gcccacgagg gcttggactg
     1081 gaagatcgcc accgtggaac cggggttccg ccgggtctcc tcgggcaatt tccaactcac
     1141 caacgaggag aacgccaaaa gccgtgaggt ctgggaagcc ctcttcggca agaagggcca
     1201 cggcaccgat tggggggatg cgaagtacct gcaatccctg gttgatggcg cgccggggcg
     1261 cctgttgaag tctttcttcg gggaccccat gagccgtccg cgcttcgcct ccttcgagcg
     1321 caccaacgag tacatctcgt cccagtcccc gcttgaggat ctgctcaaaa agcagggggg
     1381 caaaaagccg gaaccggctg ggaaccccga ggatcctaag ccctccgggt ccaaggcgac
     1441 gtgatctacg ccgttgtccc cctcgctttc ttagcggctt tcctgggggc ctacctcggg
     1501 gcgactttcg ccctcaggcg gtccccgaag cccgagccgg tggaccttgc caaatgggtc
     1561 gaagcggata aacgggctct tgcctccaag gacaccacgg aatcccggca catggacgag
     1621 tgggccgaac agcaggtgtt catggcttcc cagggtggtc tatgatctct aaaaaaccac
     1681 tcacccgaga cgcttccgcc tacgacaaaa gccctttcgt cgacctcggc tatctcgggg
     1741 accctgacca cctagtctcg gaggtcatgt ccctttacga gaacgacaaa aaagcgcggc
     1801 tcatcttcga tcggtacatt tacgaatcca ccgcccttta cgtcgggaag cataacctca
     1861 tctggaagga cggcgcctac acccccatgg ccgccgactt cctcggacag tcccaggtcg
     1921 tggtgaacaa gatcatcggg aaatgctcgg cggtggtcaa ccgtatgacc gacttcgacc
     1981 ccaccgccca agccttaccc gcttccggtt ccgtgtccga tacctatgcc gcccgcgccg
     2041 ccaagaagat cctccagtcc aaccattact cctacgattt ccagcgggtc taccggcgcc
     2101 atgctctgga tatcgtggtc cagggggacg ggttcctcaa gacgatgtac gacccttacg
     2161 gtgggcgaaa atccgtg
//
LOCUS       DASTJJ010000836         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_603341, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000836 DASTJJ010000000
VERSION     DASTJJ010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603341"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="atpG"
                     /locus_tag="VFR02_10105"
     CDS             <1..732
                     /gene="atpG"
                     /locus_tag="VFR02_10105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET9870833.1"
                     /translation="DRMAEIIRRVAAGHSGGSHPLLARRADGKDVLLVVAADRGLCGS
                     FNGNVLRRAKGFLKDHRGAEVMAIGKKARDFFRRHHVPLRRDWVQVFPEVSAETLADI
                     RDSLVSLFIHEEVRTVTVVYTEFKGVMSQKVVEETLLPLKLEEAKAAAAPAPAGPGGD
                     YLFEPDEESILNTLLAQHLLIRVRRILLESFASEMGAKRTAMEAATKNASQMIGRLTL
                     EFNRARQAMITKELAEIVGGAEALK"
     gene            883..>2283
                     /gene="atpD"
                     /locus_tag="VFR02_10110"
     CDS             883..>2283
                     /gene="atpD"
                     /locus_tag="VFR02_10110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9870834.1"
                     /translation="MSTQTTTPALTGKIAQVIGPVVDVKFTPAGIPPIFNALKVETGG
                     DPAVLVLEVSQHLGDQVVRCLAMSSTDGLKRGMDVTDTGSPITVPVGRETLGRILNVL
                     GESVDEMGEVQSAKRYPIHRPAPPLVEQSTRKQILETGIKVIDLLAPYQKGGKIGLFG
                     GAGVGKTVIIQELIRNIATQHGGFSVFTGVGERTREGNDLWLEMKESGVIEKAALVYG
                     QMNEPPGARARVALSGISIAEYFRDEENQDVLLFIDNIFRFTQAGSEVSALLGRMPSA
                     VGYQPTLATEMGALQERITSTHRGSITSVQAIYVPADDLTDPAPATSFSHLDATTNLN
                     RALTELGIYPAVDPLDSTSRILDPLVVGDEHYAVARKVQQILQRYKELKDIIAILGID
                     ELSDDDKTLVGRARRIQKFLSQPFFVAQQFTGAPGRYVPLADTIRSFKGLVEGQYDEL
                     PEQVFYMVGNIDEALER"
BASE COUNT          401 a          771 c          762 g          349 t
ORIGIN      
        1 gaccggatgg ccgagatcat ccgccgggtg gcggcggggc attcgggggg cagccacccc
       61 ctgctggccc ggcgcgcgga cggcaaggac gtgctgctgg tggtggcggc ggaccgggga
      121 ttgtgcggca gcttcaacgg caacgtgctg cgccgggcca agggcttcct gaaggaccac
      181 cggggggccg aggtgatggc catcggcaag aaggcccggg atttcttccg ccggcaccac
      241 gtacccctgc ggcgggactg ggtgcaggtc ttccccgagg tgtccgccga gaccctggcc
      301 gacatccggg attcgctggt gtcgctcttc atccacgagg aagtgcgcac cgtcacggtg
      361 gtctataccg agttcaaggg cgtgatgagc cagaaggtgg tcgaggagac cctgttgccc
      421 ctcaagctgg aggaggccaa ggccgcggcc gccccggcgc ccgcgggccc gggcggggac
      481 tacctgttcg agcccgacga agagtccatc ctcaacaccc tgctggccca gcacctgctc
      541 atccgggtgc gccgcatcct gttggaaagc ttcgcctccg agatgggggc caagcgcacc
      601 gccatggagg ccgccaccaa gaacgcgtcc cagatgatcg ggcgcctgac gctggagttc
      661 aaccgggccc gccaggccat gatcaccaag gaactggccg agatcgtggg cggcgcggaa
      721 gcgttgaaat agcccgggcc aaaacctatt tcaccgccaa ggcgccaaga cgccaaggac
      781 ggcaaaaaat ggacctgatt cggtctttct tggcgtcttg gcgtcttggc gccttggcgg
      841 tgaaacaagt ttttttttga tctcatcact tttggaggaa gcatgagcac ccaaacgacg
      901 acccccgcct tgacgggcaa gatcgcgcag gtcatcggcc ccgtggtgga cgtgaagttc
      961 accccggccg gcatcccgcc catcttcaac gccctgaagg tcgagaccgg gggggacccg
     1021 gcggtgctgg tgctggaagt gtcccagcac ctgggggacc aggtggtgcg ctgcctggcc
     1081 atgagctcca ccgacgggct caagcgcggc atggacgtga ccgacacggg gtcgcccatc
     1141 acggtgcccg tggggcggga gaccctgggc cgcatcctga acgtgctggg cgaatcggtg
     1201 gacgagatgg gcgaggtgca gtccgccaaa cgctatccca tccaccgccc ggccccgccg
     1261 ctggtggaac aatccacccg caagcagatc ctggagacgg gcatcaaggt catcgacctc
     1321 ctggcgccct accagaaggg cggcaagatc ggcctcttcg ggggcgcggg cgtgggcaag
     1381 accgtcatca tccaggagct gatccgcaac atcgccaccc agcacggcgg cttttcggtc
     1441 ttcacgggcg tgggcgagcg cacccgcgag ggcaacgacc tgtggctcga gatgaaggaa
     1501 tcgggcgtca tcgagaaggc cgccctggtc tacggccaga tgaacgagcc gccgggcgcc
     1561 cgcgcccggg tggcgctgtc gggcatctcc atcgccgagt atttccgcga cgaggagaac
     1621 caggacgtgc tgctcttcat cgacaacatc ttccgtttca cccaggcggg cagcgaggtg
     1681 tccgcgctcc tgggccgcat gccttccgcc gtgggttacc agcccacgct ggccaccgag
     1741 atgggggcgc tgcaggagcg catcacctcc acccaccggg ggtccatcac gtcggtccag
     1801 gccatctacg tgcccgccga cgacctgacc gacccggcgc cggccacgtc cttttcccac
     1861 ctggacgcca ccaccaacct gaaccgggcg ctcaccgaac tgggcatcta tccggcggtg
     1921 gaccccctgg attccacctc ccgcatcctg gaccccctgg tggtgggcga cgagcactac
     1981 gcggtggccc gcaaggtgca gcagatcctg cagcgctaca aggagctcaa ggacatcatc
     2041 gccatcctgg gcatcgacga actctccgac gacgacaaga ccctggtggg gcgggcgagg
     2101 cgcatccaga agttcctgtc ccagcccttc ttcgtggccc agcagttcac cggggccccg
     2161 ggccgctacg tgcccctggc cgacaccatc cggtccttca agggcctggt ggagggccag
     2221 tacgacgagc tgcccgagca ggtcttctac atggtgggca acatcgacga ggccctggag
     2281 cgg
//
LOCUS       DASTJJ010000837         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1583991, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000837 DASTJJ010000000
VERSION     DASTJJ010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583991"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFR02_10115"
     CDS             <1..990
                     /locus_tag="VFR02_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9870835.1"
                     /translation="AAPIVTFSIDPTGQRTNLKVGEVHIGMNQNTITQAVRYVATAII
                     LTTVMVLLLGILIMTVFVTVLIKPIRLLVSGVSAIAAGDFDQQIHIHRSDELGDLTSA
                     FNNMAKSLREKEVIKGAFSKYVTKSVVDRILQHQDGLKLGGEKKVVTVFFSDIRGFTP
                     MSEVLSAEEVVHLLNEYFTAMTSIIFKYEGTLDKFMGDAIMAVYGAPIDIPDHAERAV
                     LAAVEMGEKMKELQAKWRAEGKREVNIGIGINTGEVVVGNIGSNERMEYTAIGDNVNL
                     TQRLESVAEKGQILISSATYEKVKHKVRATLLDPIKVKGKADKVLAYSVEGLL"
     gene            1106..2365
                     /locus_tag="VFR02_10120"
     CDS             1106..2365
                     /locus_tag="VFR02_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870836.1"
                     /translation="MSSEDKNLIVKMAYLYVQGGEWYKAIEEYKKLLAMDPEDAHVFN
                     MMGDAYAKKQDDPDAFDAYMTGRALYSKQGNFGKVSSIEKKIQKLDAGRLDLKRRHVF
                     ESVTKTLEADRLASEGRPEEAVAYYQKLIQAEPINFSYREKLASLFLENAQVTDALEQ
                     LRAIADIHLEAGRLDKAQAMADQMAEMDPEGYDTLKLLGELADRKGDQDTLSRVYDKL
                     GHVAYGAGHFEEARTAIEKAMAAGHQGLKALYAKTLAALKLSAEAKPQFEALLAENPG
                     DEELTAQLLSICEDLKDWPAVQAHAASLAARHPGDAALQGRLARSLVQVGKRDEATQI
                     YLQQAALALKENKVEAVLGSFDRILSYEPDHLDILKKRAELNLKLGRKQEAIAAYKAF
                     QDALTRKKMVEEARRIGIILNKLAGLK"
     gene            2369..2674
                     /locus_tag="VFR02_10125"
     CDS             2369..2674
                     /locus_tag="VFR02_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870837.1"
                     /translation="MDPASPPAPAGRRTNLDAECLRCGAFNVAENRICGACGASLPLV
                     YDEEGNVFEWERGSSPAGRPFTPYRVDPRKTGWFLRAGVLLFALGVAALIILRGARP"
     gene            2727..>3562
                     /locus_tag="VFR02_10130"
     CDS             2727..>3562
                     /locus_tag="VFR02_10130"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HET9870838.1"
                     /translation="MRETCVEPSLTELPTKKVFLETFGCQSNALESDHVAGLLRQKRF
                     QMTADAAEADVILFNTCSIRQHAEDKVFSRLGELGEWKRGKDGRVVGVLGCMATSYKE
                     KLLERAPHLDLVVGPDQYPQVPEALERAARGGAGQVLADFDPVYFPENDPAHLSQPHK
                     AFLEIMKGCDKFCTYCVVPFTRGREVSRPAEDILAEAGRLGAAGVKDVTLLGQNVNSY
                     GLGLKSKGKTLTFARLLRQVAAVEGIERVRFMTSHPLDLSDELIEVMAGTPGVCEYFH
                     LPV"
BASE COUNT          671 a         1194 c         1153 g          544 t
ORIGIN      
        1 gccgcgccca tcgtcacctt ctccatcgac cccacgggcc agcggaccaa cctgaaggtg
       61 ggcgaggtcc atatcggcat gaaccagaac accatcaccc aggcggtgcg ctacgtggcc
      121 acggccatca tcctcacgac cgtcatggtg ctgttgctgg gcatcctgat catgaccgtc
      181 ttcgtgaccg tgctcatcaa gcccatccgc ctgctggtgt ccggcgtgtc ggccatcgcg
      241 gcgggcgatt tcgaccagca gatccacatc caccggtcgg atgaactggg cgacctgacc
      301 agcgccttca acaacatggc caagtccctg cgcgagaagg aggtcatcaa gggcgccttc
      361 tccaagtacg tgaccaagag cgtggtggac cgcatcctgc agcaccagga cggcctcaag
      421 ctgggcggcg agaagaaggt ggtgacggtg ttcttttccg acatccgggg cttcacgccc
      481 atgtcggagg tcttgagcgc cgaggaggtg gtccacctct tgaacgagta cttcacggcc
      541 atgacctcca tcatcttcaa gtacgagggc acgctggaca agttcatggg cgacgccatc
      601 atggccgtct acggcgcccc catcgacatc ccggaccacg ccgagcgggc cgtgttggcc
      661 gccgtggaaa tgggcgagaa gatgaaggaa ctgcaggcca aatggcgcgc cgagggcaag
      721 cgcgaggtga acatcggcat cggcatcaac accggcgagg tggtggtggg caacatcggg
      781 tccaacgagc ggatggaata cacggccatc ggcgacaacg tgaacctgac ccagcggctg
      841 gaatcggtgg ccgaaaaggg ccagatcctc atttccagcg ccacctacga gaaggtcaag
      901 cacaaggtga gggccaccct gttggacccc atcaaggtca agggcaaggc cgacaaggtg
      961 ctggcctaca gcgtggaagg ccttctttag ccccttttgg ccgggcgccg gcgccccgcc
     1021 gggtcctttt gtccgcctta tttctccctt ggtggcttgg cgtcttggtg gtgaaatggt
     1081 ttgaacgtga tccggaaggg gtgccatgtc ctctgaagac aaaaatctga tcgtcaagat
     1141 ggcctacctc tacgtccagg gcggggagtg gtacaaggcc atcgaggaat acaagaagct
     1201 gctggccatg gacccggagg acgcccacgt cttcaacatg atgggcgacg cctacgccaa
     1261 gaaacaggac gacccggacg ccttcgacgc ctacatgacc gggcgggcgc tctattccaa
     1321 gcagggcaat ttcggcaagg tctcctccat cgagaagaag atccagaaac tggacgcggg
     1381 ccggctggac ctcaagcgcc gccacgtctt cgagtcggtc accaagaccc tggaggcgga
     1441 ccggctggcc tccgagggcc gccccgagga ggcggtggcc tactaccaga agctcatcca
     1501 ggccgaaccc atcaatttct cctaccggga aaaactggcc tccctgttcc tggagaacgc
     1561 ccaggtcacc gacgccctgg agcagttgcg ggccatcgcg gacatccacc tggaagccgg
     1621 gcgcctggac aaggcccagg ccatggccga ccagatggcc gagatggacc cggaaggcta
     1681 cgacaccctg aagctgttgg gcgagctggc cgaccgcaag ggggaccagg acaccctgtc
     1741 gcgggtctac gacaagctgg gccacgtggc ctacggggcc gggcacttcg aggaggcccg
     1801 cacggccatc gagaaggcca tggcggcggg ccaccagggc ctcaaggccc tctatgccaa
     1861 gaccctggcg gccctcaagt tgagcgccga ggccaagccc cagttcgagg cgctgttggc
     1921 cgagaacccc ggcgacgagg agctgacggc ccaactgctc tcgatctgcg aggacctgaa
     1981 ggactggccc gcggtccagg cccacgccgc ctccctggcg gcccgccacc ccggggacgc
     2041 ggccctgcag ggccgcctgg cccggtcgct ggtgcaggtc gggaaaaggg acgaggccac
     2101 ccagatctac ctgcagcagg ccgccctggc cctcaaggag aacaaggtcg aggccgtgct
     2161 gggttccttc gaccgcatcc tgtcctacga gcccgaccac ctggacatcc tcaagaagcg
     2221 cgccgagctc aacctcaagc tgggccgcaa gcaggaggcc atcgcggcct acaaggcctt
     2281 ccaggacgcg ctcacccgca agaagatggt cgaggaggcc cggcgcatcg gcatcatcct
     2341 caacaagctc gcggggctga agtaacccat ggaccccgcc tcccccccgg ccccggccgg
     2401 gcggcgcacc aacctggacg ccgaatgcct gcgctgcggc gccttcaacg tggccgagaa
     2461 ccgcatctgc ggggcctgcg gcgccagcct gcccctggtc tatgacgagg aaggcaacgt
     2521 cttcgagtgg gaaagggggt cctcgcccgc cgggcggccc ttcacgccct accgtgtcga
     2581 cccccggaag accggctggt tcctgcgggc gggggtgctg ctcttcgccc tgggggtggc
     2641 cgcgctcatc atcctgcgcg gcgcccggcc ctagccttaa ccgcccccgc ctgctaaaat
     2701 cgggctcctg accggaccca tcccccttgc gagagacctg cgtggaaccg agcctcaccg
     2761 aacttccgac caaaaaagtg ttcctggaga ccttcgggtg ccagtccaac gcgttggaat
     2821 cggaccatgt ggccggcctg ctgcgccaaa agcgcttcca gatgaccgcc gacgccgccg
     2881 aggcggacgt gatcctgttc aacacctgct ccatccgcca acacgccgag gacaaggtct
     2941 tttcccgcct gggggaactg ggggagtgga agcggggcaa ggacggccgg gtggtgggcg
     3001 tcttgggctg catggccacc agctataagg aaaagctctt ggagcgggcg ccgcacctgg
     3061 acctggtggt ggggcccgac caatacccgc aggtgcccga agcgctggag cgcgccgccc
     3121 ggggcggcgc gggccaggtg ctggcggatt tcgaccccgt ctatttcccc gagaacgacc
     3181 cggcccacct ttcccagccc cacaaggcct tcctggagat catgaagggt tgcgacaagt
     3241 tctgcaccta ctgcgtggtg cccttcaccc ggggacggga agtgtcccgg cccgcggagg
     3301 acatcctggc cgaggcgggg cggctggggg ccgcgggcgt gaaggacgtg accctgctgg
     3361 gccagaacgt gaattcctac ggcctgggcc tcaagtccaa gggcaaaacc ctgaccttcg
     3421 cccgcctgct ccggcaggtc gccgcggtgg aggggatcga gcgcgtgcgg ttcatgacct
     3481 cccatccgct ggacctctcc gacgagctca tcgaggtgat ggcgggcacg cccggggtct
     3541 gcgagtattt ccacctgccg gt
//
LOCUS       DASTJJ010000838         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_848593, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000838 DASTJJ010000000
VERSION     DASTJJ010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848593"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..612
                     /locus_tag="VFR02_10135"
     CDS             394..612
                     /locus_tag="VFR02_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870839.1"
                     /translation="MNSFANPEDAVPFEYPEFPKAAKESFEAFNDPAVSPGFADFFAA
                     QAQPPAEVPVVQPALLQDFKDVFAATQS"
     gene            617..>2023
                     /locus_tag="VFR02_10140"
     CDS             617..>2023
                     /locus_tag="VFR02_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870840.1"
                     /translation="MEEPSRLPRFSPWAWPLAALFLLAALFACRQDLDFDIGFHLKAG
                     QWIVQHQAVPREDAFTYTVAGHPYVDMLWLYEAACYGLQRLGGLTLLSAVHDLLILAA
                     FALLTRRTFRSAAPPAVSLVLLLGALLCLELRFLMRPEALSWVFFFLAWRLLEDRHRP
                     GARPLLWLSLLQVAWVNTEGLFILQWVLVGAYALGAWWETRRPEPLLLKALGLSLLAS
                     LLNPYLFQGLLFPFTLLGHLAPGDPFHGSIAEFQSPWAPLRQQSAPFFPALPLALYKV
                     LGLGSLFLVAATWRKRAAWERLLVPVFFALSAFSIRNVPFFLFIALPTAAAALRDLLD
                     SPKPWVAGLSRRLSLPWPAPWLTLVMALLAARVSTQAYYVADRRMVHNGFGVDEQHIP
                     VKAVDFLSQFQLGGAVLNDLGFGAWLDWKGPAPVFLDGRLEVLGADFYSRFLASYQPG
                     GLKPLLDRYGVQLVLLDHM"
BASE COUNT          288 a          709 c          583 g          443 t
ORIGIN      
        1 aggccgatat agaaaggatg ggcggcctgc tcagcccggg ccgccgccgc gctcaaagcg
       61 atgagaagga acaagatgat ctttttcacg gccgcctccc cggttttttt cggattatac
      121 ccgccccccg ggcgtgaatc ccagggggga aaaagaatgg aactatggct tttacaaagc
      181 aagccaaagg gaacttgaaa caatgataaa aactgatgaa acctgatatt tgaaaataac
      241 catcaggctt tatcagcttt tatcaatgtt ccaattgtcc ttgctttgtg aagtgcctga
      301 tttaggagtt aaagctatga tccttgtgtt tttaaacaat acggtcccaa aaagatggtt
      361 tccaagcccg gaaaccgggg gtaaaatgcc cccatgaact ctttcgccaa ccccgaggac
      421 gccgtcccct tcgagtatcc tgaattcccc aaggccgcca aggaaagctt cgaggccttc
      481 aacgacccgg cggtctcccc cggtttcgcc gactttttcg ccgcccaggc ccaaccgccc
      541 gcggaagtcc ccgtggtgca gcccgccctg ctccaggact tcaaggacgt cttcgccgcc
      601 acccagtcct gaccgtttgg aagagccgtc ccgcctcccg cgtttttccc cctgggcctg
      661 gcccctggcc gcccttttcc tgctcgccgc cctcttcgcc tgccgccaag acctggattt
      721 cgacatcggc ttccacctca aggccgggca gtggatcgtc cagcaccagg ccgtcccccg
      781 ggaggacgcc ttcacctaca cggtggcggg gcatccctac gtggacatgc tctggctcta
      841 cgaggccgcc tgctacggcc tgcagcgcct gggcggcctg acgctccttt cggccgtcca
      901 tgacctgttg atcctggccg ccttcgcgct gctcacgagg cgcaccttcc gttcggccgc
      961 cccgccggcg gtctccctgg tgctgctgct gggcgcgctg ctttgcctgg agctgcgttt
     1021 cttgatgcgg ccggaggcgc tcagttgggt cttcttcttc ctcgcctggc gcctgctgga
     1081 ggaccggcac cggccgggcg cccggcccct gctttggctt tccctgctgc aggtcgcctg
     1141 ggtcaacacc gagggccttt tcattttgca gtgggtgctg gtgggggcct atgccttggg
     1201 cgcctggtgg gagacccgcc ggcccgaacc gctgctcttg aaggccctgg gcctttccct
     1261 cttggcctcg ctgctcaatc cctacctgtt ccaggggctg ctcttcccct tcaccctgct
     1321 gggccacctg gcgcccgggg accccttcca cggctccatc gccgagttcc aatccccctg
     1381 ggcgccgctg cgccagcaaa gcgcgccctt cttccccgcc ctgcccctgg ccctctataa
     1441 ggtcctgggc ctcggttcgc ttttcctggt ggccgccacc tggcgcaagc gcgcggcgtg
     1501 ggaacggctg ctggttcccg tttttttcgc cctctcggcc ttctccatcc gcaacgtccc
     1561 cttcttcctc ttcatcgccc tgcccaccgc cgcggccgcc ctgagggacc tcttggattc
     1621 acccaagccc tgggtggcgg ggctttcccg ccggttgagc ctcccctggc cggcgccctg
     1681 gctgacgctg gtcatggcgc tcttggcggc ccgggtgtcc acccaggcct attacgtggc
     1741 cgaccggcgc atggtgcaca acggcttcgg ggtggatgag cagcacatcc ccgtgaaggc
     1801 cgtggacttt ttgtcccagt tccaattggg cggcgccgtg ctgaacgacc tgggtttcgg
     1861 ggcctggctg gactggaagg gccctgcccc ggtctttttg gacggccgcc tggaagtgct
     1921 gggggcggac ttttattcgc ggttcctcgc cagctaccag cccggcggcc tgaagccctt
     1981 gttggaccgc tacggcgtgc agttggtgct gctcgaccac atg
//
LOCUS       DASTJJ010000839         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_848639, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000839 DASTJJ010000000
VERSION     DASTJJ010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848639"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFR02_10145"
     CDS             complement(<1..272)
                     /locus_tag="VFR02_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870841.1"
                     /translation="MLPKKLSFKIGFFLALSILIIEALVILSTVVQEKRKQVDEKVRL
                     LDLVTQALSGDFDRSIRYSHYLALSQTLSEVARQFGADSLVLFDHQG"
     gene            complement(366..>1618)
                     /locus_tag="VFR02_10150"
     CDS             complement(366..>1618)
                     /locus_tag="VFR02_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9870842.1"
                     /translation="GGVAAAPPGGLNDPTPGMLHYPPPRKVPMSKVYLDHAATTPLDP
                     EVLEAMLPYLKEEFGNPSSVHAWGREARAALERSREKIAFTLNADLKEIVFTSGGTES
                     VNAVHKGVAWASPGKARHFIVSAVEHSCVAETAEWLTNQGYPVTTVGCDAQGWVDPEA
                     VRRAIRPGETRLVSVMAANNEVGTLQSVREIGRLCREQGVLFHTDAVQAYGKVPLDVQ
                     KDFIDFLSVSGHKIYGPKGSGFLYQRRGLDLVPLIHGGGQERDRRGGTENVAALVGLG
                     EAAWRICNDTAERDRLRALTGLFLEELGKSLPAFRLNGPDRPERRLPGLVNITLPGME
                     GETLVHSLDAKGFAASSGPACSAGAAPPSRVLTAMGRSPKEAKQGLRLSFGRSTGEAQ
                     VKLLAGALPGIVEQLSRMASFLDE"
BASE COUNT          252 a          547 c          563 g          256 t
ORIGIN      
        1 ccctggtggt cgaacaagac gagcgaatcc gccccgaact gccgggccac ttccgacagg
       61 gtctgggaca gggccaggta atgggaataa cggatggacc ggtcgaaatc gcccgagagg
      121 gcctgggtca ccaggtcgag cagccgcacc ttctcgtcga cctgcttgcg cttttcctgc
      181 accacggtgg aaaggatgac cagggcctcg atgatgagga tggacagggc caggaagaag
      241 ccgatcttga agctcagttt tttggggagc aaggatgccc tttcgcgccg ggtgctgggc
      301 ccattttcgc cgcaaaggcg ccgggacgcc aagcgaaaac agggcccgcg gggcccgggg
      361 ggttcttatt cgtccaggaa ggaggccatg cggctcaatt gttccacgat gccgggcagc
      421 gcgcccgcca aaagcttcac ctgggcctcg ccggtggacc ggccgaagct caggcgcagg
      481 ccctgcttgg cttccttggg gctgcggccc atggcggtca gcacgcggga aggcggcgcg
      541 gcgccggccg agcaggccgg gcccgaggag gccgcgaagc ccttggcgtc caggctgtgc
      601 accagtgtct cgccctccat gccgggcagg gtgatgttca ccaggccggg caggcgccgc
      661 tcgggccggt cgggcccgtt gagccggaag gccggcaggc tcttgcccag ttcctccagg
      721 aagagccccg tgagggcgcg cagccggtcc cgctcggcgg tgtcgttgca gatgcgccaa
      781 gccgcctcgc ccagccccac cagggccgcc acgttctcgg tgccgccccg ccggtcgcgt
      841 tcctgcccgc cgccgtggat gagcggcacc aggtccagcc cccggcgttg gtagaggaac
      901 cccgatccct tggggccgta gatcttgtgg cccgagaccg agaggaagtc gatgaagtcc
      961 ttctgcacgt ccagcgggac cttgccgtag gcctgcacgg cgtcggtgtg gaacagcacg
     1021 ccctgctccc ggcagagccg gccgatctcc ctcacggatt ggagcgtgcc cacctcgttg
     1081 ttggccgcca tgaccgacac cagccgggtc tcgccggggc ggatggcgcg ccggacggcc
     1141 tccgggtcga cccagccctg ggcgtcgcag cccaccgtgg tcaccggata gccctggttc
     1201 gtgagccact cggcggtctc cgccacgcaa ctgtgttcga cggcggagac gatgaaatgc
     1261 cgcgccttcc ccggggaggc ccaggccacg cccttgtgca cggcgttcac gctttccgtg
     1321 ccgccgctgg tgaagacgat ctctttcagg tcggcgttga gggtgaaggc gatcttttcc
     1381 cgggacctct ccaacgcggc ccgggcctcc cggccccagg catgcacgct ggaggggttg
     1441 ccgaactctt ccttcaaata gggcagcatg gcttccaaca cctcggggtc caacggggtg
     1501 gtggcggcgt gatcgagata gaccttggac atgggaacct ttcgcggcgg gggatagtgt
     1561 agcatccccg gcgtgggatc gttcaacccc cccggcggcg cagccgcaac gccaccag
//
LOCUS       DASTJJ010000840         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1705480, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000840 DASTJJ010000000
VERSION     DASTJJ010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705480"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /locus_tag="VFR02_10155"
     rRNA            <1..>1629
                     /locus_tag="VFR02_10155"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          354 a          431 c          622 g          222 t
ORIGIN      
        1 tgggggtcat gcctcagcat cccgccttgt tgcgggaggg gaaccggggg aactgaaaca
       61 tctcagtacc ccgaggaaca gaaagcattc cccaagtagt ggcgagcgaa cggggaggag
      121 cccaaaccgg ccgggactga gtggcgatcg ggcctatgtc ccgggcgggg ttgtacgccc
      181 ccgcgtggcg ggccgtccga tcggcggccc ccccggaaga gcaaagggcc cgggttgcag
      241 ccgaacggcg tggaaacgcc ggccggaggg ggtgagagcc ccgtaggcga cgcgcccggg
      301 cgccaggagg cgcgggggtc gtgagtacca cgcgtgccgt ggaaccgtgt gggaagctgg
      361 gacgcccacg tcccaaggct agaataccgt cggcgaccga tagtgaacga gtaccgtgag
      421 ggaaaggtga aaagcacccc gagaggggga tgaaagagac cctgaacccc agcacccaca
      481 agcagtcgga gggagtattc tcctttgggg cgacccgggg ggaggcctga cggcgtgcct
      541 tttgtagaat gatccggcga gttgatgtcg gcagcgcgtg ccgcctttgg ggggcacgaa
      601 gaggttaagc ggcagcgacg aaagagccgt ggagccggag cgaaagcgag tctgaagagg
      661 gcgtcggagt tgccgggatc agacccgaaa ccgggtgagc tatccatggg cagggtgaag
      721 cgtgggtaag accgcgtcga ggcccgaacc caccagtgtt gcaaaactgg gggatgacct
      781 gtggataggg gagaaattcc aatcgaaccc ggagatagct ggttctcccc gaaatgtatg
      841 gaggtacagc cgtggtcgta tttgcctgcc tgggaggtag ggcactggat gggctcgggg
      901 ccgtttgccg gttaccaacc ccaaccaaac cgcgaatgcc caggtgaggg gaccacgagt
      961 gaggcggcgg gggctaacct tcgtcgccga gaggggtaca acccagatcg ccagctaagg
     1021 gccccaagta tccgctgagt ggacaaggta gtgggggggc agagacagcc aggaggttgg
     1081 cttagaagca gccatccttg aaagagtgcg taatagctca ctggtcgagt gttcctgcgc
     1141 cgacaataac acggggctca aggcgcgcgc cgaagctgcg gatggccgct gtgggtggtg
     1201 acaccccggc cgtggtaggg gagcgttcgg gagtagcgga gaagggtgac tgggaagcca
     1261 cctggagcga accgaagcgc gaatgtcgga atgagtagcg agcatggtgg tgcgaaccca
     1321 ccacaccgga agcctaaggg ttcctgggca agggcaatcg ccccagggtc agccgggacc
     1381 taagccgagg ctggtagcag cgtaggcgat ggcgagcagg tagacagtcc tgcgcccgtg
     1441 gcgccagacg acgatggggg gacgcagcag gcaaggtcag cccaccgaat ggacagggtg
     1501 ggtcgcggcc gggcggtggg ggtccaggga aagccggacc ccagtcaaca ccaaacggct
     1561 gggcgagtcg ccgctgaggc gacggaagtg acgtggtggc caagctgccg agaaaagcct
     1621 ctagtcgat
//
LOCUS       DASTJJ010000841         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_974016, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000841 DASTJJ010000000
VERSION     DASTJJ010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974016"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /gene="ptsP"
                     /locus_tag="VFR02_10160"
     CDS             complement(<1..1148)
                     /gene="ptsP"
                     /locus_tag="VFR02_10160"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9870843.1"
                     /translation="MSEKILHGVAASGGIAIAPAYLYHPSGLAVPRRALKAEETEAEV
                     QRYLNAIAKTRIQIGEVAEKARLRLGDEHASIFQAHQVVLDDPVFQQEIPEAVRVRRL
                     NAEALIHEQLEKFRELIAGIDDPYFRDRGNDIQDVGRRMLRNLIGGEKPQLRKLDHEV
                     ILISEDLSPSDTVNLPTDVIKGFATEIGGRTSHVAIVARSLGLPAIVGMPGITGEVQD
                     GDLLILDGNKGLVILDPTPETLKQYQRLQESYRAFQKSLEELKNLPATTPDGRRITLA
                     ANIEIPQELNEIFLHGAEGVGLFRTEFLFLDRDNVPTEEEQYLAYRELAEKIGVHPAI
                     VRTLDLGGDKFLSHLNTLHESNPFLGLRAIRLCLKRPEIFMPQLRAMLR"
     gene            complement(1152..1424)
                     /locus_tag="VFR02_10165"
     CDS             complement(1152..1424)
                     /locus_tag="VFR02_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9870844.1"
                     /translation="MKASEKMPIINKLGLHLRAAAELVKVANKFKCQIFIHHGVQNVN
                     AKSLMGLMTLAAAKGTELEFAAEGDDAKDALAAIRDLLAKRFGEKE"
     gene            complement(1461..>1995)
                     /locus_tag="VFR02_10170"
     CDS             complement(1461..>1995)
                     /locus_tag="VFR02_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HET9870845.1"
                     /translation="NALKVGMMGPLAAMGDNLFWATVRPYCGLLAVTLVLSKSFFGGG
                     QYWVIPLLFLVVYNVAHVGLRWMGFWQGYRKSDQVVLTLRQYGFQQAIRGLRLAAVVL
                     LGVLIVFVNLSDLEHRAYLFTLKLTFFTAIVGLFTFVLHKKISPSQVFYAVVLFALML
                     AFWPDLSATPDPLAGQP"
BASE COUNT          338 a          624 c          670 g          363 t
ORIGIN      
        1 cgcagcatgg cccgcaactg gggcatgaag atctcgggcc gtttcaggca caaccggatg
       61 gcgcgcaggc ccaggaaggg gttgctctca tgcagcgtgt tcaggtggga caggaacttg
      121 tcgccgccca ggtcgagcgt gcgcacgatg gcgggatgca cgccgatctt ctcggccagt
      181 tcccggtagg ccaggtattg ttcctcctcg gtgggcacgt tgtcgcggtc caggaacagg
      241 aactcggtgc ggaacaggcc cacgccctcg gccccatgca ggaagatctc gttgagctcc
      301 tgggggatct cgatgttggc cgccagggtg atgcgccgcc cgtcgggggt ggtggcggga
      361 aggttcttga gctcctccag gcttttttgg aaggcccggt aggattcctg caggcgctgg
      421 tattgcttga gggtctcggg cgtggggtcc aggatgacca ggcccttgtt gccgtccagg
      481 atgagcaggt cgccgtcctg cacctcaccg gtgatgcccg gcatgcccac gatggcgggc
      541 aggccgaggg aacgggccac gatggccacg tgggaagtgc gcccgccgat ctcggtggcg
      601 aagcccttga tgacgtcggt gggcaggttg accgtgtccg aaggggatag gtcctcggag
      661 atcaggatga cctcatggtc gagcttgcgc agttggggct tctcgccccc gatcaggttg
      721 cgcagcatgc ggcggcccac gtcctggatg tcgttgccgc ggtcgcggaa ataggggtcg
      781 tcgatgcccg cgatgagctc gcggaacttc tccaactgct catggatgag ggcctcggcg
      841 ttcaggcgcc tcacgcgcac cgcctcgggg atctcctgtt ggaacaccgg atcgtccaac
      901 accacctggt gggcctggaa gatggaggcg tgctcgtcgc ccagccgcag ccgggccttt
      961 tcggccactt cgccgatctg gatgcgggtc ttggcgatgg cgttcaggta gcgctgcacc
     1021 tcggcctcgg tctcctcggc cttgagggcg cggcggggca cggccaggcc cgacgggtgg
     1081 taaaggtagg ccggggcgat ggcgatgccg ccggaagcgg ccaccccgtg caggatcttt
     1141 tcggacaaag cctactcctt ctccccgaat cgtttggcca acaggtcccg gatggcggcc
     1201 agggcgtcct tggcgtcgtc gccctcggcg gcgaactcaa gttccgtccc cttggcggcg
     1261 gccagcgtca tgagccccat caggctcttg gcgttgacgt tctgcacccc gtggtggatg
     1321 aagatctggc acttgaactt gttggccacc ttcaccagtt cggcggcggc cctcaggtgc
     1381 agccccagtt tgttgatgat cggcatcttc tcgctggctt tcatggctcc ctcttccctt
     1441 ctaatatcaa aacggctgat ttacggctgg cccgccaagg ggtccggcgt ggcgctcagg
     1501 tccggccaga aggccagcat gagcgcgaac aggaccaccg cgtaaaagac ctggctgggg
     1561 ctgatcttct tgtgcagcac gaaggtgaac agccccacga tggcggtgaa aaacgtcagc
     1621 ttcagggtga acaggtaggc ccggtgttcc aggtccgaca ggttcacgaa cacgatcaac
     1681 acgcccaaca gcaccacggc ggccagccgc aatccccgga tggcctgctg aaaaccgtac
     1741 tggcgcaggg tgagcaccac ttggtcgctc ttgcggtagc cctgccagaa acccatccac
     1801 cgcaggccca cgtgggccac gttgtagacc accaggaaca gcagggggat gacccaatac
     1861 tgcccccccc cgaagaagga cttggacagc accagggtca cggccagcag cccgcaatag
     1921 gggcgcacgg tggcccagaa aaggttgtcc cccatggcgg ccagggggcc catcatgccc
     1981 actttcaacg cgttg
//
LOCUS       DASTJJ010000842         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_240271, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000842 DASTJJ010000000
VERSION     DASTJJ010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240271"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFR02_10175"
     CDS             <1..441
                     /locus_tag="VFR02_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9870846.1"
                     /translation="EKLCRQQGIPLTVQRRAILEALVGREDHPTADQIYEAVTHRLPG
                     LSRATVYRVLETLVEVGVIHRASHLGAAARYDPNTERHHHLTCVKCHKVVDLEEEALS
                     AIHLPAGLQGFEIMDYSVHLQGYCPDCRKRTKPEKKPPTRAKTA"
     gene            468..947
                     /locus_tag="VFR02_10180"
     CDS             468..947
                     /locus_tag="VFR02_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET9870847.1"
                     /translation="MEPNIGISSKGREAVGKLLNTLLSDEVALYVKTRNFHWNVAGPH
                     FNDLHKFFEGQYEALDEILDDVAERARALGVPAYGSFTEFSRATRLKENPSSRPLSAP
                     EMVRQLLADHEAVIRNLRRDHEVPAKLGDAGTDDFLVGLIEEHEKMAWMLRAMAGKG"
     gene            1086..1610
                     /locus_tag="VFR02_10185"
     CDS             1086..1610
                     /locus_tag="VFR02_10185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9870848.1"
                     /translation="MPAQVGQKAPDFQGQALMPNGEFKDIKLGDYKGKWVVLFFYPLD
                     FTFVCPTEIKSFNEKYEEFKKMGAEVITASTDSVYSHKAWQETPALGKLKFPHLADTA
                     HHISRAYGVLIEDKGIALRGTFVIDPEGNLKHATINDTAVGRNVEETIRTLAAFETGK
                     LTGCGWTPGQKTLN"
BASE COUNT          366 a          545 c          557 g          265 t
ORIGIN      
        1 gaaaagctct gccgccagca ggggatcccc ctgacggtcc agcgccgggc gatcctggag
       61 gccctggtgg gccgggaaga ccatcccacc gccgaccaga tctacgaagc cgtcacccac
      121 cgcctgcccg ggctgtcccg cgccaccgtc taccgggtcc tggagaccct ggtggaggtg
      181 ggcgtcatcc accgggccag ccacctgggc gcggccgccc gctacgaccc caataccgag
      241 cgccaccacc acctgacctg cgtgaagtgc cacaaggtgg tggacctgga agaggaggcg
      301 ctgtcggcca tccacctgcc cgccgggctg cagggttttg agatcatgga ctattcggtg
      361 cacctgcagg gctactgccc ggactgccgc aagaggacca aaccggaaaa aaagcccccg
      421 acgcgggcca agaccgcctg acccatttca cgaaaggagc agaaatcatg gaaccgaaca
      481 tcggcatctc cagcaaggga cgggaggcgg tggggaagct cttgaacacc ctcttgtccg
      541 atgaggtggc gctctacgtc aagacccgca acttccattg gaacgtggcc gggccccact
      601 tcaacgacct gcacaagttc ttcgagggcc agtacgaggc cctggacgag atcctggacg
      661 acgtggccga acgggcccgg gcgctgggcg tgccggccta cgggtccttc accgaatttt
      721 cccgggccac ccggctcaag gagaacccct ccagccggcc cttgagcgcc cccgagatgg
      781 tccggcagct tttggccgac catgaggccg tcatccggaa cctgcggcgg gaccacgagg
      841 tcccggccaa gctgggggac gccggcacgg acgatttttt ggtggggttg atcgaggagc
      901 acgagaagat ggcctggatg ctgcgggcca tggccgggaa gggctgaggc cggccccggg
      961 ggaacgcgct gtgcccggtg aaaaccgggt aaaaaaataa aaaggggctt gcaagccccg
     1021 gtggggcctt tggatactga ggcgcacgag gttttgaagg ccgctataaa ttcaaggagg
     1081 acaagatgcc tgctcaagtc ggacaaaagg ccccggattt ccagggtcaa gcgctgatgc
     1141 ccaacggcga attcaaggat atcaagctgg gcgactacaa ggggaagtgg gtggtgttgt
     1201 tcttctaccc gctggacttc accttcgtgt gccccacgga gatcaagagc ttcaacgaga
     1261 agtacgagga gttcaagaag atgggcgccg aggtcatcac ggcctcgacc gacagcgtct
     1321 acagccacaa ggcttggcag gaaacgccgg ccctgggcaa gttgaagttc ccccacctgg
     1381 cggacacggc gcaccacatc tcgcgcgcct acggcgtcct gatcgaggat aagggcatcg
     1441 ccttgcgcgg caccttcgtc atcgacccgg agggcaacct caagcacgcc accatcaacg
     1501 acacggccgt gggccgcaac gtggaggaga ccatccgcac cttggcggcc ttcgagaccg
     1561 gcaagctgac gggctgcggc tggacccccg gccagaagac gctcaactaa aagcgggcag
     1621 ttcaaaagcc cttgggccca aaggcccaag ggcttttttt attttgggac cgtatgaaag
     1681 gttctccgct tcttcctcga ggggagaagg ttgggatgcg cccaaagcgg cac
//
LOCUS       DASTJJ010000843         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1461123, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000843 DASTJJ010000000
VERSION     DASTJJ010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461123"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..875)
                     /locus_tag="VFR02_10190"
     CDS             complement(165..875)
                     /locus_tag="VFR02_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870849.1"
                     /translation="MDLTAFRADWEAAVEALLQHHPAPSRVSALLPELQEVDPDPAAQ
                     FGPPPTPTPQRVEVARTGGPQGVSVKPINVTAIQADPQATLAADQTQTGPNALRDLVN
                     LAQIEAFQNPLIAGQAVSAAITTLANPAPDAANRNNNLQRLLNALESLGMTDRADALL
                     PIVLQHATQDAQTAQANFDSLSADQQNRPVQVGGQAANEFRQVARLNLHAAIAAAQKL
                     NTPAVEPLILAAIASQIR"
     gene            923..>1515
                     /locus_tag="VFR02_10195"
     CDS             923..>1515
                     /locus_tag="VFR02_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870850.1"
                     /translation="MSQKVDRLAEAGRILIGLGGGVSHVEQGGVAGLVERSGKWAGTG
                     RDHDAAALQQHQGGLIGEGAVGLLTLGDQGKRVKIEAGSGGQKGERRVEEGIVEGGFA
                     DVGVLDEVEDGFELRLSAGAAMAYVRGRKGTDEVGFDAFFRVVRDPVGGSVGGQIRRW
                     EGDADDLLEDRNQIRPSLLLPLLVGWQGARFIEGDGL"
BASE COUNT          240 a          377 c          617 g          281 t
ORIGIN      
        1 atggtggtgc cggcgctgaa ggtggcgggg ttcaacttca ccgaagtgac gaaattctaa
       61 cgcgggcacc cggcctcagg aacccggggc gggggcccct tcgccccgcc cctcccctcg
      121 caggatagag attgccagcg aaccgatctt cggccggggt ggcgttagcg gatttgggag
      181 gcgatggcgg ccaggatcag gggctcgact gccggggtgt tgagtttttg ggcggcggcg
      241 atggcggcgt ggaggttgag acgggcgact tggcggaatt cgttggcggc ttggccgccg
      301 acttggacgg ggcggttttg ctggtcggcg gagaggctgt cgaagttggc ctgagcggtt
      361 tgggcgtcct gggtggcgtg ctgcagaacg atgggcagca gggcgtcggc gcggtcggtc
      421 atgcccaagc tttcgagggc attcagtaag cgttggaggt tgttgttacg gttggcggca
      481 tctggggctg gattggccaa cgtggtgatg gccgccgaga ccgcctggcc agcaatcagc
      541 gggttttgaa acgcttcgat ttgcgccaag ttgaccaggt cgcgcagggc attgggcccg
      601 gtctgggttt ggtccgcggc cagtgtggct tgcgggtcgg cctgaatggc ggtcacattg
      661 atgggcttga cgctgacacc ttgtgggccg cccgtgcggg ccacctcgac ccgctgcggg
      721 gttggggtgg gcggtgggcc gaactgggca gcggggtccg ggtccacctc ttgcagttcg
      781 ggcaagaggg ccgaaaccct ggagggggcg ggatgatgct gcaggagcgc ctcgaccgca
      841 gcttcccagt cggcgcggaa ggcggtgagg tccaccggga agcgggggcg gcggggttgg
      901 ccggcgacgg gatcgagcgg cgttgtcgca aaaagttgac cgcctcgccg aggccggccg
      961 gatcctgatc ggcctcggcg gcggcgtcag ccatgtggag cagggcggtg tcgccggcct
     1021 ggtcgagaga tccgggaaat gggctggaac cggccgagac catgacgccg ccgcgttgca
     1081 acaacaccag ggcggcctga taggggaagg agccgtcggc ctgctgacac tgggcgatca
     1141 aggcaagcgg gtcaaaattg aggccgggtc gggcgggcaa aaaggcgaga gacgagttga
     1201 ggaaggcatc gtagaggggg gctttgcgga cgtcggcgtt ctggatgagg tcgaggacgg
     1261 ttttgaattg cgactgagcg ctggcgccgc catggcgtat gtacgtggca gaaagggcac
     1321 ggacgaggtt ggcttcgatg ccttctttag ggttgtaagg gatccagtcg gcgggagcgt
     1381 tggggggcag attaggcgtt gggaaggtga cgccgacgat ctgctggaag atcgaaatca
     1441 aatccgtccg agcctgttgc tgccattgtt ggtcggttgg cagggtgctc gtttcatcga
     1501 aggcgatggt ttgga
//
LOCUS       DASTJJ010000844         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1827529, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000844 DASTJJ010000000
VERSION     DASTJJ010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827529"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFR02_10200"
     CDS             complement(<1..339)
                     /locus_tag="VFR02_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870851.1"
                     /translation="MKRMKLWLAGGLLASLAVYPLAALADDGAGAPPPDDGAMQGPPP
                     EGMGEMGGGKWLDTMKDKLGLSDSQVGQIKKNLEDQKNALKPIREDIRTQTKVLAEKV
                     KAGAKDQDLKP"
     gene            572..817
                     /locus_tag="VFR02_10205"
     CDS             572..817
                     /locus_tag="VFR02_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET9870852.1"
                     /translation="MSQPIIVYTTSWCGDCHRLTRQLDERGIAYEQRDIERDPADFKT
                     MLSYTNGKRVIPTVDIGGKILINPRLSQVLETLGLPA"
     gene            complement(814..996)
                     /locus_tag="VFR02_10210"
     CDS             complement(814..996)
                     /locus_tag="VFR02_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870853.1"
                     /translation="MTYTQFERWLERLAPRDLDYLKRHKEWAKEYEKARRTGILPAGF
                     PERTMEVLEDGSDSET"
     gene            complement(1056..1274)
                     /locus_tag="VFR02_10215"
     CDS             complement(1056..1274)
                     /locus_tag="VFR02_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870854.1"
                     /translation="MSPRVRFHLETPAPQPSLRVYTVAFREVLMIPLAPLAPGDHDIP
                     LGKLSLANGLYYLEVPGETRPVKWLVLR"
     gene            1402..2118
                     /locus_tag="VFR02_10220"
     CDS             1402..2118
                     /locus_tag="VFR02_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870855.1"
                     /translation="MTVGTAVGEVVGVKVGLAVEEEVGVGLLEGVAEGMAVPVAVGVP
                     VPVGVAVVGTVGLGEGVPVGEGLAVEVPVTVGEGPAVKVEVVVGLGIGESVGVEEGVP
                     VGDKVAVAVAVGVGLGDAVGETDGDGDKVTVGVWVTVGVWVELAVRVGEVVGLGVRVP
                     VGDRVGVGTGVTVRVGVGVSATAGSTTTTPAMPVQAVWQVGDTPQSPWMVQWKLKVPA
                     WEKTWVTEKPGARVPASPMS"
BASE COUNT          441 a          794 c          924 g          431 t
ORIGIN      
        1 gggcttcagg tcttggtcct tggcaccggc cttcaccttt tcggccaaaa ccttggtctg
       61 ggtgcggatg tcctcccgga tgggcttcag ggcgttcttt tggtcttcca ggttcttctt
      121 gatctggccc acctgggagt cgctcaagcc cagcttgtcc ttcatggtgt ccagccattt
      181 cccgccgccc atctccccca tcccctcggg cggaggtccc tgcatggccc catcgtccgg
      241 gggcggggcc cccgcgccgt cgtcggccaa agccgccaag ggataaaccg ccagggaggc
      301 cagtaagccc cccgccaacc aaagtttcat tcttttcatg ccatcctcct cgatgcttcc
      361 atgggaatgg gatcggctcc cctttccctt ccacccattg gacgccggga gggtcggcgg
      421 ggggtccaca ggaagaccgc tcggagcctg gaaaattttg ggatgtagga ggattttcgg
      481 aacttcaagg ccgcccagaa cgtccgcgtc cggcgccttg aaaagacggc ggcggcgccc
      541 cacaatgcct gaaagacccc gggagaccca catgagccaa cccatcatcg tctacaccac
      601 gtcctggtgc ggcgactgcc accgcctgac ccgccagttg gacgagcgcg gcatcgccta
      661 tgagcaacgg gacatcgagc gggaccccgc cgatttcaag accatgcttt cctacaccaa
      721 cggcaaacgc gtcatcccca cggtggacat cggggggaag atcctcatca acccccgcct
      781 gtcccaggtg ttggagaccc tggggcttcc ggcctaggtc tcgctgtccg atccgtcctc
      841 cagcacctcc atggtgcgct cggggaaacc cgcgggcagg atccccgttc tgcgggcctt
      901 ctcatattcc ttggcccatt ccttgtgccg tttcaggtaa tccaggtccc ggggcgctag
      961 gcgctccagc caccgctcga attgggtgta agtcattgtt gcctcccgcc gcggatgccc
     1021 acgaagccct tccaggataa ggccgacccc ctccctcagc gcaagaccaa ccatttgacc
     1081 ggccgggttt cccccgggac ctccaggtaa tagaggccgt tcgccaggga aagtttcccc
     1141 aaggggatat cgtgatcgcc cggggcaagc ggggccaagg ggatcatcaa cacctcccgg
     1201 aaggccaccg tatagacccg cagggacggc tggggggcgg gcgtttccag gtggaaccgg
     1261 acccgcgggg acacgcgccc atcccaggga tttggataga ccaccggacg cgccactggc
     1321 gtggccgtcg gctgaggggg cgtcgaagtg gcggacgggg tgggcgtcgg ggaagtcgtg
     1381 acggtccgcg taggggtccc cgtgacggtc ggtaccgccg tgggtgaggt tgtcggcgtg
     1441 aaggttgggc ttgccgtcga ggaagaagtg ggtgtcggac tattggaagg cgtggcggaa
     1501 gggatggccg tcccggtcgc ggtgggggtc ccggtcccgg tgggcgtagc ggtcgtaggg
     1561 acggtggggc tgggcgaggg ggtgccggtc ggcgaggggc tggccgtgga agtcccggtc
     1621 acggtgggcg aagggccggc cgtgaaagtg gaggtggtag taggactggg gatcggggaa
     1681 agcgtggggg tcgaggaagg ggtcccggtc ggcgacaagg tggccgtggc cgtcgcggtg
     1741 ggggtcgggc tgggggacgc ggtgggcgaa accgatgggg atggggacaa ggtcacagtg
     1801 ggggtttggg tgacggtcgg cgtttgggtg gagctggccg tgcgggtcgg ggaggtcgtc
     1861 gggctgggcg tcagggtccc ggtcggcgac agggtcggcg tgggtacagg ggtgacggtc
     1921 cgggtggggg tcggggtgag cgccaccgcc ggatccacca ccactacccc cgccatgccc
     1981 gtgcaggcgg tctggcaggt gggggatacc ccgcaaagcc cgtggatggt gcagtggaaa
     2041 ttgaaggtcc cggcctggga aaagacctgg gtgacggaaa aaccaggggc cagggtcccc
     2101 gcgtccccca tgtcgtagaa agcgcaggtc cctgtgcccg cgccattgtc caccgacacg
     2161 ttgtggaagc ccgcgttgta gacccaggcc accgattggc cctgggtgat ggtgacggcg
     2221 gcggggttga agacgttgct gagggcctgt cccaccgtca cgaaggccgc cggggtctgg
     2281 gcccgcgccg gcgccgcggg ccagccgagc aaaagcgcga gaaagaacgg ggcggcccgc
     2341 cttttcccgg tggacgggaa cgccgaaaga acgccgaaag accagccgcg tctcacgccc
     2401 gcctccggaa gatggaccgc gccccggggc cgcggccccg cgcatcgtca cttattttaa
     2461 gaccatatac cgtccttcca gaaggctgtc aaggaacggc gggcgaaatg cccagagggc
     2521 aaaaaaaggc ccggccaaga agttacaaaa aaattcagcg gacgttattt tcccggacga
     2581 cagcggcacc
//
LOCUS       DASTJJ010000845         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1827575, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000845 DASTJJ010000000
VERSION     DASTJJ010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827575"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFR02_10225"
     CDS             <1..349
                     /locus_tag="VFR02_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9870856.1"
                     /translation="AEKTAQDWLAAWNAHDLEAILSHDDPAIVFTSPFVGPLTGGISG
                     TLAGLEKLRDHFSKALAAYPDLAFRPVNVLAGVSSLVVYYESVGGRMSAELMELNPAG
                     KIARVLAHDGPPR"
     gene            complement(346..>1133)
                     /locus_tag="VFR02_10230"
     CDS             complement(346..>1133)
                     /locus_tag="VFR02_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9870857.1"
                     /translation="PFHAEWALAAARAGKHILCEKPLTLHFPAARRVIQAAKQHQVFF
                     MEAFMYRCHPQTAQLADLVRRKAVGELKLIQASFCIDRPVDLKGRLFNRALGGGAIWD
                     LGCYPVSMARLLAGAALGKPFADPVEVKGLGLVGGQSRVDEIALGSLKFPGGILAEVS
                     CATRLRRPVGVTLYGTKGTLHVPSPWHADRFKKGAARIRVERDGQRPREIAVRAGQNL
                     YALEVDAVGAALKAGKLESPFMGWADSLGNAKVLDAWMRAVGL"
BASE COUNT          183 a          390 c          381 g          179 t
ORIGIN      
        1 cgccgaaaag accgcgcaag actggctcgc cgcctggaac gcccatgacc tggaagccat
       61 cctgtcccat gacgatcccg ccatcgtctt cacgtcgccc tttgtcgggc ccttgacggg
      121 ggggatttcc ggcaccctgg caggtctgga aaaattgcgg gatcatttct cgaaagcgtt
      181 ggccgcctat ccggacctgg ctttccggcc ggtcaacgtc ttggcgggcg tctccagcct
      241 ggtggtctat tacgagagcg tgggcggacg gatgtcggcc gagctgatgg aactcaaccc
      301 ggccgggaag atcgcgcggg tcttggccca tgacgggccg ccgcgttaaa ggccgaccgc
      361 gcgcatccag gcgtccaaca ccttggcgtt gcccaggctg tcggcccagc ccatgaaggg
      421 cgattccaac ttgcccgcct tcaaggccgc ccccacggcg tccacttcca gcgcgtagag
      481 gttttggccg gcgcgcaccg cgatctcccg gggcctttgg ccgtcccgct ccacgcggat
      541 gcgggcggcg cccttcttga agcggtcggc gtgccaaggc gaggggacgt gcagggtgcc
      601 cttggtgccg tagagggtca cccccacggg acggcgcaac cgggtggcgc aggagacctc
      661 ggccaggatg ccgcccggaa acttgagcga tccgagggcg atctcgtcca cgcggctctg
      721 gccccccacc aaccccaatc ccttcacttc caccggatcg gcgaaaggct tgcccaaggc
      781 cgccccggcc aggagcctgg ccatggagac cggatagcag cccaggtccc agatcgcgcc
      841 gccccccagg gcccggttga agagccggcc cttcaggtcc accggccggt cgatgcagaa
      901 ggaggcctgg atgagcttga gctcccccac agccttgcgg cgcaccaggt ccgccaactg
      961 ggcggtctgg gggtggcagc ggtacatgaa ggcctccatg aagaagacct ggtgctgctt
     1021 ggccgcctgg atgacccgcc gggccgcggg aaagtgcagc gtgagcggtt tctcgcagag
     1081 gatgtgcttg cccgcccggg cggccgccaa ggcccattcg gcgtggaaag gat
//
LOCUS       DASTJJ010000846         1335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_484101, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000846 DASTJJ010000000
VERSION     DASTJJ010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484101"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFR02_10235"
     CDS             <1..341
                     /locus_tag="VFR02_10235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9870858.1"
                     /translation="WWPRSIRFQVDPGPVKVGTRMRVSNQGLVRWTAEVTDIQENKKI
                     MFRYFDGAWDGTAAWTLTPEGSGTRVAYSIDIVPVPFWLKALSGVLDLGKIHSKMMAG
                     VLASLQKMFI"
     gene            complement(334..1173)
                     /locus_tag="VFR02_10240"
     CDS             complement(334..1173)
                     /locus_tag="VFR02_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9870859.1"
                     /translation="MKTTFLTLAALLCFAANSLLGRAALGGGFSDAATYTAVRLASGA
                     LTLFLVLSTGKVRESRPPQFDPLSALALFAYAAGFSFAYLRLTAGTGAVLLFGAVQAT
                     MIGWGLASGEKWRPREAAGLLLALGGVGLMTFPGITAPDPWGAGLMVLAGVAWGAYSL
                     RGRSAGGPIHRTAFNFYGSLPLAAVLFLACRGKIHATAEGLALAALSGALASGAGYCF
                     WYAALPRLTAFRAAVVQLATPLLAALGGILLLGEPAGWRVFTSGAAIAAGVLLAVSRP
                     KAK"
BASE COUNT          272 a          421 c          461 g          181 t
ORIGIN      
        1 gttggtggcc caggtccatc cgtttccagg tcgaccccgg gccggtcaag gtcgggacac
       61 gcatgcgcgt ttccaaccag ggcctggtgc gctggacggc agaagtcacg gacatccaag
      121 aaaataaaaa gatcatgttc aggtatttcg acggggcctg ggacggcacc gccgcctgga
      181 ctctgacgcc cgaggggtcc ggcacccggg tggcctattc catcgacatc gtgccggtgc
      241 ccttttggct caaggccctc tccggcgttc tggacctggg gaagatccat tccaagatga
      301 tggccggggt cctggcatcc cttcaaaaaa tgttcattta gctttcgggc gggaaacggc
      361 taaaagcacg cccgccgcaa tggccgcccc cgaggtgaag acccgccagc cggcgggttc
      421 gcccaagagc aggatgccgc ccagggccgc caacaggggt gtggcgagct ggacgacggc
      481 ggcccggaag gccgtcaacc ggggcagggc cgcgtaccaa aagcagtaac ccgcgccgga
      541 tgccagggcg cccgagaggg cggccaaggc cagcccttcg gcggtggcgt ggatctttcc
      601 ccggcaagcc aggaaaagga cggccgccag gggaagggaa ccgtaaaagt tgaaggcggt
      661 ccggtggatc gggccgccgg ccgagcggcc ccgcagcgaa taggcccccc aggccacccc
      721 ggccaggacc atgagccccg cgccccaagg atcgggcgcc gtgatcccgg gaaaggtcat
      781 cagccccacg cctcccaggg ccagtagaag tccggcggct tcccgcgggc gccatttctc
      841 ccccgaggcc aacccccagc cgatcatggt ggcctggacc gcgccgaaga gcagcaccgc
      901 cccggtcccc gccgtgagcc taagataagc gaaggaaaaa cccgccgcgt aggcgaagag
      961 ggccagggcc gagagggggt cgaattgcgg ggggcgggat tcccgcacct ttccggtgga
     1021 gaggacgagg aagagggtca gggcgcctga agccaggcgg accgcggtgt aggtggcggc
     1081 gtccgaaaag cccccgccca gtgccgcgcg gcccagcagg gaattggccg cgaagcagag
     1141 cagggcggcc agggtcagga aggtggtctt catggcgggg acatcttatc attgaaaagt
     1201 gctaagggat tttgacgacc gccggatttt acggcatccc atcggataaa atgacctgga
     1261 ctcgaaggat cgtaccggcc cgtcgaaccg gaattggagg atgagatcat gaggactgaa
     1321 tcccaacccg cctac
//
LOCUS       DASTJJ010000847         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_484122, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000847 DASTJJ010000000
VERSION     DASTJJ010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484122"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFR02_10245"
     CDS             complement(<1..891)
                     /locus_tag="VFR02_10245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9870860.1"
                     /translation="MTTLPKTYGLYFFVLLLPLTNGWVPWLVPGLISARTTDTFLAWM
                     PYWGLGLVGLLGLQLNQTRLLAAALWTMAAYFLMRQPGFFRGLGLSPLRDLEVLGVTY
                     PLSLGLLFSFKESRFFSAETLFRLLLAFLPAAFLAGLLWVDPLGYQGLLDLGLPAVTA
                     WTRVPTLAWLFGLGLAGFVYFWKESKTKPFLAALTLGLTAFYFALAESLKAYREPFSP
                     LPLAAVVLSALALTAVLLHAAFHLYWNRVYLDPLTGIPNRQALEDRLHTLSGRFTLAM
                     MDIDHFKHFNDNWGHQTGDQV"
     gene            1039..>1402
                     /locus_tag="VFR02_10250"
     CDS             1039..>1402
                     /locus_tag="VFR02_10250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9870861.1"
                     /translation="MNILHVLSGDFVTGAETSTAAVIERQLARGHRVFIASGAFPHAT
                     KAVWVRVPVYDRRPWSRLANILALARLVRREDIDLLHAHSRAASWVSHWAAKLTGRAY
                     LSTLHGRQHLHFSNIHFNI"
BASE COUNT          281 a          428 c          486 g          207 t
ORIGIN      
        1 cacctggtcc ccggtctggt ggccccagtt gtcgttgaaa tgcttgaagt ggtcgatgtc
       61 catcatggcc agggtgaagc gtcccgacag ggtgtgcagg cggtcctcca aggcctggcg
      121 gttggggatg ccggtcaagg ggtccaggta gacccggttc cagtataggt ggaaagccgc
      181 gtgcaacaag accgccgtca gggccagggc ggacaacacc acggccgcca ggggaagcgg
      241 ggaaaagggt tcccggtagg ccttcaggct ctcggccagg gcgaaataaa aggccgtcaa
      301 gcccagcgtg agcgcggcca ggaagggttt ggtcttggat tccttccaaa agtaaacgaa
      361 gccggccagg cccaacccga aaagccaggc cagggtgggg acccgggtcc aagcggtcac
      421 cgcggggagc cccaggtcca gcagcccctg gtagcccaag ggatccaccc acaaaagtcc
      481 cgctaggaag gccgccggca ggaaagccag caggagccgg aagagggtct cggcgctgaa
      541 gaagcggctt tctttgaaag agaacaggag cccgagggag aggggatagg tgacgcccag
      601 gacctccagg tcgcgcagcg gtgacaggcc caggccccgg aagaagccgg gttggcgcat
      661 gaggaaatag gcggccatgg tccaaagggc cgcggccagg agccgcgttt ggttcaactg
      721 gagccccaag aggcccacca ggcccaaccc ccaatagggc atccaggcca ggaaggtgtc
      781 ggtggtgcgg gccgagatca ggcccggcac cagccagggg acccagccgt tggtcagggg
      841 cagcagcagc acgaagaagt agaggccgta ggttttgggg agcgtggtca cggggtggtc
      901 ctggtcctgg gaatggcggc gcccggaagg gggatttcta ttttgcaaac gaagtccccc
      961 aaaagccaat gaaatttggg gccctttttg ccccaaaaga aggggatggg ttatcatggg
     1021 cgccggaaag gggcggcctt gaacatcctc cacgtcttga gcggcgattt cgtcaccggc
     1081 gccgaaacct ccaccgccgc cgtcatcgag cggcagctgg cccgcgggca ccgggtcttc
     1141 atcgcctcgg gcgccttccc gcacgccacg aaggccgtct gggtccgggt gccggtctat
     1201 gaccggcggc cctggagccg tcttgccaac atcctggccc tggcccgcct ggtgcggcgg
     1261 gaggacatcg acctgctgca cgcccattcc cgggccgcca gctgggtgag ccattgggcg
     1321 gccaagctca cgggccgggc ctacctttcc accctgcacg gcaggcagca cctgcatttt
     1381 tccaacatcc acttcaacat tt
//
LOCUS       DASTJJ010000848         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_604414, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000848 DASTJJ010000000
VERSION     DASTJJ010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604414"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..955)
                     /locus_tag="VFR02_10255"
     CDS             complement(17..955)
                     /locus_tag="VFR02_10255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9870862.1"
                     /translation="MSVFWRRVFLGVAGLLSVLWLGLVGLLLKQGFPSWFASAPPKPS
                     VAARSVLPNGSGWAPLDGVPGVQFRVEKVQSGDTYASLAKQCGLAETTLRSLNQANDG
                     SQPKAGSSLLIPSRDGIFHVAAPGQGLSDIARAYGIPLKSILESNRLSGDEGLVGRVL
                     VLPGAAYLDHKDVHWLALQALSDERGFLKPTTGRFADGFGPRINPISGKKEFHPGLDL
                     APGWHARVVAAQSGEVVFAGIRAGYGRLIILDHGGGLTSRYAHLDQILVKLHQRVQRG
                     DLIGRVGMTGMATGPHLHFEVRLNDVPQNPLIYLTQ"
     gene            complement(959..>1133)
                     /gene="rho"
                     /locus_tag="VFR02_10260"
     CDS             complement(959..>1133)
                     /gene="rho"
                     /locus_tag="VFR02_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144699.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9870863.1"
                     /translation="RREELLMTDADLNKVWILRKILNPNNPVEAMEFLLDKMKRTKSN
                     KAFLESMKGGVSD"
BASE COUNT          198 a          361 c          391 g          183 t
ORIGIN      
        1 ggaccggacc aaggacttat tgggtcaaat agatcagggg gttttggggt acgtcgttca
       61 accggacctc gaaatgcagg tgggggcccg tggccatgcc ggtcatgccc acccgtccga
      121 tcaggtcccc ccgttggacc cgctggtgga gcttgaccag gatctggtcc aaatgggcgt
      181 accgggaggt caggccgccc ccgtggtcga ggatgatgag ccgtccatac ccggcgcgga
      241 tcccggcgaa gaccacctcc ccggactggg cggccaccac ccgggcatgc catcccggcg
      301 ccaggtccag cccgggatgg aactccttct tgccgctgat gggattgatc cggggcccga
      361 atccatcggc gaaccgcccg gtggtgggct tcaggaatcc cctttcgtcc gaaagcgcct
      421 gcagggccag ccaatggacg tccttgtggt ccaggtaggc cgcccccggc aggacaagga
      481 cccgtcccac cagtccctcg tcccccgaca ggcggttgga ctccaggatg gacttcaagg
      541 ggatgccata ggcgcgggcg atgtcggaca ggccctggcc cggggccgcg acgtggaaga
      601 tgccgtcccg ggacgggatg agcaaggaag agccggcctt cggttgggag ccgtcgttgg
      661 cctggttgag gctgcgcagg gtggtttcgg ccaggccgca ttgcttggcc agggaggcgt
      721 aggtgtcccc gctttggacc ttctcgaccc ggaactggac gcccgggacg ccgtccaacg
      781 gggcccaccc ggagccgttc ggaagcacgg accgggccgc gacggaaggt ttcggggggg
      841 cggaggcgaa ccaagagggg aaaccctgct tcagcagcag ccccaccaac cccagccaga
      901 ggaccgacag gagaccggcg acccccagga agacccgccg ccaaaagacc gacatgggtt
      961 agtcgctgac gccgcccttc atggattcca agaaagcctt gttggacttg gtgcgcttca
     1021 ttttgtccag caggaactcc atggcctcca cggggttgtt ggggttgagg atcttgcgca
     1081 ggatccagac cttgttcagg tccgcgtcgg tcatgagcag ctcctcgcgg cgg
//
LOCUS       DASTJJ010000849         1447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_974344, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000849 DASTJJ010000000
VERSION     DASTJJ010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974344"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..131)
                     /gene="cutA"
                     /locus_tag="VFR02_10265"
                     /pseudo
     CDS             complement(1..131)
                     /gene="cutA"
                     /locus_tag="VFR02_10265"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
     gene            146..298
                     /gene="cutA"
                     /locus_tag="VFR02_10270"
                     /pseudo
     CDS             146..298
                     /gene="cutA"
                     /locus_tag="VFR02_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
     gene            329..>1447
                     /locus_tag="VFR02_10275"
     CDS             329..>1447
                     /locus_tag="VFR02_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9870864.1"
                     /translation="MASLETTHQVFQSLIRTRTVCIILLGLLLFTVEGGLQAEAGCVI
                     LMVAAVFSLLFALLGRSKHLAAALLPFVFPLDSLFLSAWVGISGGATSYYLPLFMLIL
                     ASAVLVLPPRLALVVLPVCGAIFLGSFYLDYAGEVPAFFDASQVNRFAAQLASAPPGE
                     RAAFYWEQGLRWFFYSALMVTVCVLLMRQVWSREEKIHARERHLEQKRRLIQLGELTG
                     RIAHGVNTPLGLISGNLELLMARTRKGSPAHRSLSQIRQYVDRAVKTVRGILDYSRLS
                     LSEVRPVDLAEVARTVAEAVDPKLKKTGGKLILDLQPGLPPLLAYPEGLFQVLLNLVE
                     NAVDSVPPGRGVVTVAARFQPHPVRLSVQDDRGSLQVTV"
BASE COUNT          203 a          504 c          483 g          257 t
ORIGIN      
        1 cgcgcgggct tgaggctctc cgtgatccaa cgcaggtagg cggggttgcc ggcggcgacc
       61 cgcagggcca ggatctcgca gaccgtgtaa ggatggttct cccgcagcag cttggaaagc
      121 cgggcccaat ggaacgcgcg cgtcccattg ggcccggctt tccaagctgc tgcgggagaa
      181 ccatccttac acggtctgcg agatcctggc cctgcgggtc gccgccggca accccgccta
      241 cctgcgttgg atcacggaga gcctcaagcc cgcgcgccgc gccgggcggc ggccttgagg
      301 aaaaggacgg caatatccag gaggccccat ggcctccctt gagaccaccc accaggtctt
      361 ccagtcgctc atccgcaccc gcaccgtctg catcatcctc ttgggcctgt tgctcttcac
      421 cgtggaaggc ggcctgcagg ccgaggcggg ctgcgtcatc ctgatggtgg cggcggtctt
      481 cagcctgctc ttcgccctgt tgggccgctc caagcacctg gcggcggccc tgctgccctt
      541 cgtcttcccg ctggacagcc tcttcctcag cgcttgggtg ggcatctcgg ggggcgccac
      601 cagttactac ctgcccctct tcatgctgat cctggccagc gccgtgctgg tgctgccgcc
      661 ccggctggcg ctggtggtgt tgccggtctg cggcgccatt ttcctggggt ccttctacct
      721 ggattacgcg ggcgaggtcc ccgccttctt cgacgcctcc caggtcaacc gtttcgcggc
      781 ccaattggcc tcggcgccgc ccggggaaag ggcggccttt tactgggagc agggtctgcg
      841 gtggttcttt tattcggcgc tcatggtgac ggtctgcgtg ctgctcatgc gccaggtctg
      901 gagccgggag gagaagatcc acgcccggga acgccatttg gaacagaagc gccggctcat
      961 ccaactgggc gaactgaccg gccgcatcgc ccacggggtc aacacgccct tgggcctcat
     1021 ctcgggcaac ctggaactgc tcatggcccg cacccgcaaa gggagcccgg cccaccggtc
     1081 cctgtcccag atccgccagt acgtggaccg ggcggtcaag accgtgcggg gcatcctgga
     1141 ctacagccgc ctgagcctgt cggaagtgcg gccggtcgac ctggcggaag tggcccgcac
     1201 ggtggccgag gccgtggacc ccaaattgaa gaagaccggc ggcaagctca tcctggacct
     1261 gcagccgggc ctgccccccc tgctggccta tccggagggc ctgttccagg tgttgctcaa
     1321 cctggtggag aacgcggtgg actcggtccc cccgggacgg ggggtggtca cggtggcggc
     1381 ccggttccag ccccatccgg tgcgcttgag cgtccaggac gaccggggca gcctgcaggt
     1441 caccgtg
//
LOCUS       DASTJJ010000850         1101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1339826, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000850 DASTJJ010000000
VERSION     DASTJJ010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339826"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFR02_10280"
     CDS             <1..778
                     /locus_tag="VFR02_10280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9870865.1"
                     /translation="YKALQGVRGRKSVDLGALERLLVQFSRLVAEQPLIKEMDINPLL
                     ASPDGLVAVDARVVLHDPQADPTSLPRPAIRPYPVQYVKTVALKDGTKVTFRPIRPED
                     ETLMHAFHQSLSERSVYMRYFEPLHFSERVAHERLIRICFIDYAREMALVADYQDASG
                     KHEILGVGRLSPGRDPLEREFSLLVSDQWQNRGLGSALLSELAGIGRSEGLAAIQGHI
                     LPENQPMRRLCERLGFEIRLSGDSRNLEARLTLAGAPGKK"
BASE COUNT          213 a          368 c          326 g          194 t
ORIGIN      
        1 ctacaaggcc ctgcagggtg tccggggacg gaagtccgtg gatctggggg ccctggaacg
       61 cctgttggtg cagttcagcc ggctggtggc cgagcaaccc ctgatcaagg aaatggacat
      121 caatcccctg ctggcctcgc ccgatggcct ggtggccgtg gatgcccggg tggtcctaca
      181 tgacccccag gccgacccga cttccctgcc ccgccccgcc atccggccct acccggtcca
      241 atacgtaaag accgtggcgc tgaaggatgg gaccaaggtc actttccgcc ccatccggcc
      301 cgaggacgag accctgatgc acgccttcca ccaaagcctt tcggaacgca gcgtttatat
      361 gaggtatttc gagcccctcc atttttccga acgggtggcc catgagcggc tgatccgcat
      421 ttgtttcatc gattacgccc gggaaatggc cctggtggcc gattaccagg acgcctcggg
      481 caagcatgag atcttggggg tgggccgcct gagcccgggc cgggacccat tggagcggga
      541 attctccctc ttggtgagcg accaatggca gaaccggggc ctgggatcgg cgcttttgag
      601 cgaattggcc gggatcggcc ggtcggaggg cttggcggcc atccagggcc acatcctccc
      661 cgagaaccaa cccatgcggc gcctttgcga gcggttgggc ttcgagatcc gcctatccgg
      721 tgacagccgg aacctggagg cccgcctgac cctggccggg gcccctggaa aaaaataaac
      781 ccgaaaagga attgtcccgc gtcctaaggg ggtgggataa tgagttgaga tccaccgccg
      841 ttccaacaag aaccagcccg ccctcctgcc cgcagccgga cggacccttt cacccttccg
      901 gtgccggcgt cctttccccc gcttccggcc cggaccctca tccggggagg cccccatgcc
      961 cgaccatgac gataagaaga aacccgtcga cgcctttttc aaccggcaca aggacagttg
     1021 gaagaccaag gtctaccaag agctgaaggt caaggtctcc atccagaaat tcttcaaacc
     1081 ctcgcaacat ctgcggatcc c
//
LOCUS       DASTJJ010000851         1348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1340004, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000851 DASTJJ010000000
VERSION     DASTJJ010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340004"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..>1348)
                     /locus_tag="VFR02_10285"
     CDS             complement(224..>1348)
                     /locus_tag="VFR02_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9870866.1"
                     /translation="TPCVYPMIPLTVSYFGGQRAERPGAVLAKALAYVVGISVTYSGL
                     GVTAALTGRILGSTLQSPWVLGGISALLAALSLSMFGLYEIQAPGWLLNKVAGGQARG
                     LLGAFGMGLVFGVVAAPCVDPFSIGLLTFVAAKADPFLGFVLFFTLSMGLGFPYLWLG
                     FFSGEIQKLPRSGVWMVWVKKVFGMVLLGMPLYFLNPLMPRGLNHWLVTLYLAAAGIL
                     LGWVFSGGAVAAGFKGFQRVFGAGLAALGLAVFLLWPKPLELPFAAYDPSALAEAKQG
                     GKPLLVDFSAAWCLPCHELDLKTFSDPRVRRALKGWVLLKADLTQYGSAPVEALKKQY
                     GIMGVPTLVFVGRDGMEKKDLRAVGFVTPEELLGKVEADR"
BASE COUNT          292 a          458 c          419 g          179 t
ORIGIN      
        1 catccgggac tacaactaca aatatgacgc cgtgtcgggc atgaaggaat tgatgtggcg
       61 ctgcgggaaa tgcggggagt tggtgcaccg caaggacggg cttccggaca agtgccattc
      121 ctgcggggcg tccaagcggg aatttttcct ggtcgaagag gactgacgga ataaaaaagg
      181 ggcccgtccg ggtccctttt ttattccacc ggcgcccgaa aggtcaccgg tccgcttcca
      241 ccttgcccag aagctcctcc ggcgtcacga accccaccgc gcgaaggtcc tttttctcca
      301 tgccgtcccg gcccacgaac accagggtgg gcacccccat gatcccatac tgcttcttga
      361 gcgcctccac gggcgccgag ccgtattggg tcaggtccgc cttcaagagc acccacccct
      421 tcaaggcccg gcgcacccgg ggatcggaaa aggtcttcag gtccagctca tggcagggca
      481 ggcaccaggc cgccgaaaag tcgaccaaca ggggcttgcc cccttgcttc gcctccgcca
      541 gggccgaggg gtcgtaggcc gcgaagggca attccagggg cttgggccaa agcaggaaca
      601 ccgccagccc cagggccgcc aaccccgcgc cgaagacccg ttggaatccc ttgaaccccg
      661 ccgcgaccgc cccgcccgag aagacccatc ccaacaggat gccggccgcc gccaggtaga
      721 gggtgaccag ccagtggttc aggccccggg gcatcaaggg gttcaggaaa taaagcggca
      781 tgcccaagag caccatcccg aagaccttct tcacccagac catccagacc cccgagcggg
      841 gcaatttttg gatctcgccc gagaagaagc ccagccacag gtaggggaac cccaacccca
      901 tggacagggt gaagaagagc acgaaaccca ggaaggggtc cgccttggcg gccacgaagg
      961 tcaaaagccc gatggaaaag gggtccacgc agggcgcggc caccacgccg aagaccaggc
     1021 ccatgccgaa ggctcccaag agtcctcggg cctggccccc cgccaccttg ttgagcagcc
     1081 atccgggcgc ctggatctca tagagcccga acatggagag ggacagggcg gccaagaggg
     1141 ccgagatgcc cccaaggacc caaggcgatt gcagggtcga ccccaggatg cgcccggtca
     1201 gggccgccgt cacgcccagg cctgaatagg tcaccgagat gcccaccacg taggccaggg
     1261 ccttggccaa gacggctccg ggcctctcgg cccgctggcc cccgaaataa gagaccgtca
     1321 gggggatcat gggatagacg cagggcgt
//
LOCUS       DASTJJ010000852         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_727808, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000852 DASTJJ010000000
VERSION     DASTJJ010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727808"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="guaA"
                     /locus_tag="VFR02_10290"
     CDS             complement(<1..611)
                     /gene="guaA"
                     /locus_tag="VFR02_10290"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9870867.1"
                     /translation="MKHDLIIVLDFGAQYSQLIARRVREAKVYCEILPYHTPVAQILA
                     RAPRGIILSGGPASVYAKGAPHVDPALFQAGVPLLGICYGMQLMSQALKGRVQKASHR
                     EFGLAPLQVKSPRALFARLPSTLKVWMSHGDQVTRLPGGFKTLASTSTCPFAAAADEK
                     RKLYGIQFHPEVVHTQMGSRLLENFVLRIAGARPTWTMGSYERT"
     gene            complement(746..>1936)
                     /gene="guaB"
                     /locus_tag="VFR02_10295"
     CDS             complement(746..>1936)
                     /gene="guaB"
                     /locus_tag="VFR02_10295"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9870868.1"
                     /translation="MISNPITLSPDKRVSDALDIMKHYHISGIPVVQADGKLVGIVTN
                     RDLRFVKKVETLLSDVMTKDDLVTVPEGTTLEKAEETLHQHRIEKLLVVDKQGNLAGL
                     ITIKDINKRIKYPNACKDERGRLRVGAAVSVSADTQERVEALVAVGVDVVVVDTAHGY
                     SEGVLKTVQSLRKKYPKLNLIAGNIVTAEAAKALADLGVDAVKVGVGPGSICTTRIVA
                     GVGVPQVSAIVECAKVARKYKIGLIADGGIKYSGEIAKALVAGADAVMLGNLFAGTEE
                     SPGEVINLQGRMFKVYHGMGSLAAMQKGRSADRYFQENDAETKKLVPEGIEARVPYRG
                     QLSDLVFQLVGGLRSSMGYCGSKTIAELKKKSQFVRITPAGFQESHPHDVVITKEAPN
                     YYMD"
BASE COUNT          311 a          605 c          655 g          365 t
ORIGIN      
        1 gtgcgctcgt aggagcccat cgtccaagtg ggcctggcgc ccgcgatcct gagcacgaaa
       61 ttctccaaca ggcggctgcc catctgggtg tggaccacct cggggtggaa ctggatgccg
      121 tagagctttc gtttctcgtc ggcggcggcc gcgaaggggc aggtcgaggt ggaagccagg
      181 gtcttgaagc ccccaggcag gcgcgtgacc tggtcgccgt ggctcatcca aaccttcagg
      241 gtcgagggca ggcgggcgaa aagcgcccgg ggggacttca cctgaagggg cgccaagccg
      301 aactcgcggt gggaggcctt ttggacccgg cccttcaggg cctggctcat gagctgcatg
      361 ccgtagcaga tgcccaagag gggcacgccg gcctggaaga gggccggatc cacatggggg
      421 gcgcccttcg cgtagacgct ggcgggcccc ccggacagga tgatgcccct gggggcgcgg
      481 gccaggatct gggccaccgg cgtgtggtag ggcaggatct cgcagtagac cttggcttcc
      541 cgcacccggc gggcgatgag ttggctgtac tgggcgccga aatccaggac gatgatgagg
      601 tcgtgtttca cggaatctcc ggacttaaag ataaaaccag ttcaccgcaa aggcgcaaag
      661 ccgccaaggt gatgaaaaaa accaagttac tcgtcatcgc caaagatccg ccctttgcgt
      721 cttcgcggct ttgcggtgaa cgggttcagt ccatgtagta gttcggggct tccttggtga
      781 tgaccacgtc gtggggatgg ctttcctgga aacccgcggg ggtgatgcgc acgaactggg
      841 actttttctt gagttcggcg atggtcttgg agccgcaata acccatgctg gaccgtaagc
      901 cccccaccag ttggaagacc aggtcggaga gctggccccg gtagggcacc cgggcctcga
      961 tgccctcggg caccagtttc ttggtctcgg cgtcgttctc ctggaaatag cggtcggcgc
     1021 tgcggccctt ctgcatggcg gccagggacc ccatgccgtg atagaccttg aacatgcggc
     1081 cctgcaggtt gatgacctcg ccgggcgatt cctcggtgcc ggcgaagagg ttgcccagca
     1141 tgacggcgtc ggcgccggcc accagggcct tggcgatctc gcccgagtac ttgatgccgc
     1201 cgtcggcgat gagcccgatc ttgtacttgc gggccacctt ggcgcactcg acgatggcgg
     1261 acacctgggg cacgcccacc ccggccacga tgcgggtggt gcagatggag cccgggccca
     1321 cgcccacctt gacggcgtcc acacccaggt cggccagggc cttggcggcc tcggcggtca
     1381 cgatgttgcc cgcgatgagg ttgagcttgg ggtatttctt gcgcagggac tggacggtct
     1441 tgagcacgcc ctccgagtaa ccgtgcgccg tgtccaccac caccacgtcc acccccacgg
     1501 ccaccagggc ctccacccgc tcttgggtgt ccgcggacac gctgacggcc gcgcccaccc
     1561 gcaggcggcc ccgttcgtcc ttgcaggcgt tggggtactt gatgcgcttg ttgatgtcct
     1621 tgatggtgat gaggcccgcc aggttcccct gcttgtccac caccaacagc ttctcgatgc
     1681 ggtgctggtg cagggtctct tcggccttct ccagggtggt gccctcgggc acggtcacca
     1741 ggtcgtcctt ggtcatcacg tcggacagca gggtctcgac cttcttcacg aagcgcaggt
     1801 cgcggttggt gacgatgccc accagtttac cgtccgcttg gaccaccggg atgcccgaga
     1861 tgtggtagtg cttcatgatg tccagcgcgt cggacacccg cttgtcgggc gacagggtga
     1921 tggggttgct gatcat
//
LOCUS       DASTJJ010000853         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_849503, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000853 DASTJJ010000000
VERSION     DASTJJ010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849503"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="mutS"
                     /locus_tag="VFR02_10300"
     CDS             <1..1137
                     /gene="mutS"
                     /locus_tag="VFR02_10300"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9870869.1"
                     /translation="HRKQTLVNAERFITPELKEMEGKVLGAQEKADRMEYELFDAVRR
                     EVGRELPRLQRMARVLAELDVFACMAFIADRGRHVRPEVVDSAEMAILDGRHPVLDRL
                     LSSDQFVPNDTKLGVEGVEIMVLTGPNMAGKSTYIRQVALLTLMAQVGFYVPAKQMTL
                     GVVDRIFSRVGASDALTQGQSTFMVEMVETANILNHATPKSLLILDEVGRGTSTYDGV
                     SIAWAVVEYLAQKLRARTLFATHYHELTRLAGAYPNVRNFNIAVREWNEQILFLRKIV
                     EGGTDKSYGIQVARLAGVPKEVVARAKKILAGLESGSFLSAASKAPAAEAPAPEAPAA
                     PQMSFFGSGGDHPVLQELKGLEVDDLTPRDALNLIAEWKKKIRD"
     gene            1157..>1577
                     /locus_tag="VFR02_10305"
     CDS             1157..>1577
                     /locus_tag="VFR02_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HET9870870.1"
                     /translation="MQMKKDSSKKPMTLSNVEFLFKHKKIDMNPKYQREAVWKLSQKQ
                     LLIDSILRKIDIPKLYFRSISKGSYQYEVVDGQQRIRAIVEFMSGEYKLSDDCEDLDG
                     KSIASFSYSKLPIELQMEFNNSQLDIVVMNEAYTDDDI"
BASE COUNT          362 a          453 c          476 g          286 t
ORIGIN      
        1 caccgcaagc agaccctggt gaacgccgag cgcttcatca cgcccgagct caaggaaatg
       61 gaagggaagg tcttgggcgc ccaggaaaag gccgaccgga tggaatacga gctcttcgac
      121 gccgtgcgcc gggaagtggg cagggaactg ccccggctgc agcgcatggc ccgcgtcctg
      181 gcggaactgg acgtcttcgc ctgcatggcc ttcatcgccg accggggccg ccacgtgcgc
      241 cccgaggtgg tggactcggc cgagatggcc atcctggacg gccgccatcc cgtgttggac
      301 cgcctgctct cgtccgacca gttcgtgccc aacgacacca agctgggggt ggaaggcgtg
      361 gagatcatgg tgctgaccgg acccaacatg gcgggcaaga gcacctacat ccgccaggtg
      421 gccctgctca cgctcatggc ccaggtgggt ttctacgtgc ccgccaagca gatgacgctg
      481 ggcgtggtgg accgcatctt ttcccgggtg ggcgcctccg acgccctgac ccagggccag
      541 agcaccttca tggtggagat ggtggagacc gccaacatcc tcaaccacgc cacccccaaa
      601 agcctgctga tcctggacga ggtgggccgg ggcacctcca cctatgacgg ggtctccatc
      661 gcctgggcgg tggtggaata cctggcccaa aagctacggg cccgcaccct cttcgccacc
      721 cactaccacg aattgacccg gctggcgggc gcctacccca acgtgcgcaa cttcaacatc
      781 gcggtgcggg aatggaacga gcagatcctg ttcctgcgca agatcgtgga gggcggcacg
      841 gacaagagct acggcatcca ggtggcgagg ctcgccgggg tgccgaagga agtggtggcc
      901 cgggccaaga agatattggc ggggctggaa tcgggcagct tcctgtccgc cgcgtccaag
      961 gcgccggccg ccgaggcccc cgccccggag gcgcccgccg ccccccagat gtccttcttc
     1021 ggctcgggcg gggaccatcc cgtgctgcag gaactcaagg gcctggaagt ggacgacctc
     1081 accccccggg acgcgctcaa cctcatcgcc gagtggaaga agaagataag agactgacgt
     1141 aattttggag gtggtcatgc aaatgaaaaa agactcaagt aaaaaaccta tgactctttc
     1201 gaatgttgaa tttttattca agcacaaaaa aattgatatg aacccaaaat atcaacgaga
     1261 ggctgtctgg aaactttctc aaaaacaact cttgattgac tcgatactca gaaagataga
     1321 cattccaaaa ctttacttta ggagtatttc gaaggggagc tatcagtatg aggtggtgga
     1381 cgggcagcag cgcataaggg ccatagttga atttatgagt ggcgaataca aattatcgga
     1441 tgactgtgaa gatttggacg gaaaatcaat cgccagtttt tcttattcta aacttccgat
     1501 tgaactgcaa atggagttta acaactctca gctggacatc gtggtgatga atgaggcgta
     1561 cacagatgat gatattg
//
LOCUS       DASTJJ010000854         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1585430, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000854 DASTJJ010000000
VERSION     DASTJJ010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585430"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1052
                     /locus_tag="VFR02_10310"
     CDS             21..1052
                     /locus_tag="VFR02_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9870871.1"
                     /translation="MKAIILVGGQGTRLRPLTDHLPKNIVPLCGAPFLHYPLRQLKKA
                     GIREVAFSVGYKSQAIRKVYGDGRKLGMRFHYALEKEPLGTGGAIKNAERFVKGHPVV
                     VLNGDILTDLPLSKMIAFHRKKRSAATLGLVRVEDPTAYGLVLLDRQKRVTRFLEKPT
                     AKEAVTDTINAGVYLFEPQVFGFLPAGIQYSAERGLFPDLLASRTPFFGYVWDGYWPD
                     IGTPRKYLTTHWDILKGAFTPPAPFRKKGGVHWGKGVKLGQGARVMGPAMLGDGCVLG
                     AGARVGPLTVLGRGCRIGEGAVVEKSVLWDGASVGPGVRVKECLLGRRARVVSPPEPG
                     LVLGDSVRR"
     gene            1052..1363
                     /locus_tag="VFR02_10315"
     CDS             1052..1363
                     /locus_tag="VFR02_10315"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9870872.1"
                     /translation="MEPGFYRAASLDELEDGRSAAVEVGVMPLMLHRRGAQVFATGVF
                     CPHAEVRLDPANCFGDEIVCKAHGYHSNIRTGECLTDPDLRLPTYPVRVLEGEVWVKF
                     Y"
     gene            1494..>2478
                     /locus_tag="VFR02_10320"
     CDS             1494..>2478
                     /locus_tag="VFR02_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870873.1"
                     /translation="MSLRSTTVYGCVLLGLVLGRGFADEPTSQFYLDKGNVYIRDGKN
                     ETAIKYLQEAVDLDPYNVEARTNLGRLYAQKDQQDDALTQFQKALQVQPNYFPALSNA
                     SFVYLQKRDFPQAISYAQRALQINPDFGSAHYNLGMAYFDTGRYADSIPELRVAARAF
                     PDNAEISERLGDAYTALKHPDEALAYYRQAVNESPMSARARAKLADTYAAEGEQSRAL
                     DEYKAAVDINPGDIEAHYKLADHYKSIRDWDNAQKQYLAILQADDEQAQAHQEAAKIY
                     EREGENGKALYHWEKYAELAPGDQAAKAHIEEIRKPLLSKKEADQLAKLEQG"
BASE COUNT          460 a          783 c          820 g          415 t
ORIGIN      
        1 aataaattcg ggagcgtcac atgaaggcca tcatcctggt ggggggacag gggacccggc
       61 ttcgccccct caccgaccat ctgcccaaga acatcgtgcc cctctgcggg gcgcctttcc
      121 tgcactatcc cctgcggcag ttgaagaagg cgggcatccg ggaggtggcc ttttcggtgg
      181 gctacaagtc ccaggccatc cggaaagttt acggcgacgg ccgcaagctg gggatgaggt
      241 tccattacgc gttggaaaag gaacccctgg gcacgggcgg cgccatcaag aacgccgagc
      301 gcttcgtgaa ggggcacccg gtggtggtcc tcaatgggga catcctcacc gacctgccgc
      361 tttccaagat gatcgccttc caccggaaga aaaggtccgc cgccaccctg gggctggtgc
      421 gggtggagga ccccacggcc tacggcctgg tgctgttgga ccggcaaaag cgggtcaccc
      481 ggttcctgga aaagcccacg gcgaaggaag ccgtcaccga caccatcaat gccggggtct
      541 atctcttcga accgcaggtc ttcggtttcc tgcccgccgg gatccagtat tcggctgagc
      601 gcggcctttt tcccgacctg ttggcctccc ggacgccctt cttcggttat gtctgggacg
      661 gctactggcc ggacatcggc accccccgta agtacctgac cacccattgg gacatcctga
      721 agggcgcctt cacgccgccc gcgcctttcc gcaagaaagg cggggtccat tgggggaagg
      781 gcgtgaagct gggccagggc gcgcgggtga tggggccggc catgttgggg gacggatgcg
      841 ttttgggcgc gggcgcccgt gtggggccgc tcaccgtcct gggcaggggc tgccgcatcg
      901 gggaaggggc ggtggtggaa aagagtgtgc tgtgggacgg ggcgtcggta ggcccggggg
      961 tccgggtgaa ggagtgtctt ttggggcggc gggcccgcgt cgtttcccca cccgagcccg
     1021 gcctggtgct gggcgactcg gtccgccggt gatggaaccg gggttttacc gggcggcgtc
     1081 cctggacgag ttggaggacg gccggtcggc ggcggtggaa gtgggcgtca tgcccttgat
     1141 gctgcaccgc cggggcgccc aggtcttcgc cacgggcgtc ttttgccccc atgccgaggt
     1201 ccggttggat ccggccaact gtttcggcga cgagatcgtc tgcaaagccc acggctacca
     1261 ttccaacatc cgcacggggg aatgcctcac cgaccccgac ctgcgcctgc ccacttatcc
     1321 ggtacgcgtg ctggaaggcg aggtttgggt gaagttttat tgagggaagc cccgggcggc
     1381 cccaaatgcg gggcatcggg ggcgcctttg gccgttgacg gcccccagcc cctggcctaa
     1441 aatcgtttgt ctgttcttcg ttcttccacc attccaaccg ccaaggagcg ggcatgtccc
     1501 tgcgatcgac aacggtttat ggctgcgtcc tcttgggcct cgtcctggga cggggcttcg
     1561 cggacgagcc gaccagccag ttctacctgg acaagggcaa tgtctatatc cgggacggca
     1621 agaacgagac cgccatcaag tacctgcagg aggcggtgga cctggacccc tacaacgtcg
     1681 aggcccgcac caacctgggc cggctttacg cgcagaagga ccagcaggac gacgccctga
     1741 cccagttcca gaaagccctg caggtgcagc cgaattattt ccccgcgctt tccaacgcct
     1801 ccttcgtcta cctccagaag cgggattttc cccaggccat cagctacgcc cagcgggccc
     1861 tgcagatcaa cccggatttc ggctcggccc actacaacct gggcatggcc tatttcgaca
     1921 ccggccgcta tgcggattcc atccccgaat taagggtggc ggcccgggct ttccccgaca
     1981 acgcggagat cagcgaaagg ttgggggacg cctatacggc cctgaaacac ccggatgagg
     2041 cgctggccta ttaccgccaa gccgtgaacg agtcgcccat gtcggcccgg gcgcgggcca
     2101 agttggccga cacctacgcg gccgaggggg aacagtcccg ggccttggac gagtacaagg
     2161 ccgcagtgga catcaatccg ggggacatcg aggcccacta taagttggcg gatcactaca
     2221 agagcatccg cgactgggac aacgcccaga aacaatacct ggccatcctg caggcggacg
     2281 acgaacaggc ccaggcccac caggaagccg cgaagatcta cgagcgggaa ggggagaacg
     2341 gaaaggccct gtaccattgg gaaaaatacg ccgaactggc gcccggcgac caggccgcca
     2401 aggcccatat cgaggagatc cggaagcccc tgttgagcaa gaaggaagcc gaccagttgg
     2461 ccaagttgga acaaggca
//
LOCUS       DASTJJ010000855         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1217435, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000855 DASTJJ010000000
VERSION     DASTJJ010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217435"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFR02_10325"
     CDS             <1..353
                     /locus_tag="VFR02_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870874.1"
                     /translation="NAIQAMEKGGRLTLKTAVVEGLAPESDGKHNEALVFQKLFLKQR
                     MAVISVSDTGCGIPRENMAKLFHPFFTTKITGTGLGLSICHKIISSHGGTLDVQSVVG
                     KGSTFLIYLPLEEE"
     gene            373..762
                     /locus_tag="VFR02_10330"
     CDS             373..762
                     /locus_tag="VFR02_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9870875.1"
                     /translation="MEMGKYTILLAEDEEASRLLYTEELTEAGFRVLEAANGLQVLGL
                     LEDEKVDLLMTDIKMPDMHALEMIPRVREEHPDLPILVASAFKGMEDDFALKGCRIQG
                     FFTKPVNLEALKGEIGRLLASRPVAAA"
BASE COUNT          243 a          318 c          350 g          239 t
ORIGIN      
        1 tgaacgccat ccaggccatg gaaaagggcg ggaggctcac tttgaaaacg gccgtcgtgg
       61 agggcctggc gcccgagtcc gatggaaagc acaacgaggc gctggtcttc caaaaattgt
      121 tcctcaaaca aagaatggcc gtcatttcgg tgtccgatac gggttgcggc atcccgcggg
      181 agaacatggc caaactgttc catcctttct tcaccacgaa gatcaccggg accgggttgg
      241 gtctctccat ctgccataag atcatctcgt cccacggcgg cacgctggac gtgcaaagcg
      301 tcgtggggaa ggggagcact tttttgattt atcttccgtt ggaggaagaa taaccgatcc
      361 gggggtggcg tcatggaaat ggggaaatac acgatcctgc tggccgaaga cgaggaagcc
      421 tcgcgcttgc tctataccga agagctgacc gaggcgggtt tccgggtgtt ggaagccgcc
      481 aacgggttgc aggtgttggg gctgttggag gatgagaagg tggatctttt gatgaccgac
      541 atcaagatgc cggacatgca tgccttggaa atgatcccac gggtccggga agagcatccc
      601 gacctgccca tcctggtggc ctcggccttc aagggcatgg aggacgattt cgcgctgaag
      661 ggctgccgca tccaggggtt cttcaccaaa ccggtcaatc tggaggccct caagggggag
      721 atcgggcgcc tcttggcatc ccggccggtg gcggccgcct aaaggcggcc cggcgggtcc
      781 acaaattggt agagtgccct ccggggtccc gttttgtccc tcggctttcc cgtaaactcc
      841 gcgttgtgac aacgtaaaaa tcacgcccca tacccccagg ggtatgaagt ttgcttgaaa
      901 atccctaccg ggtattgtaa tttaacaacc caatcgaccc cttttacctt cggcggcccc
      961 tggattgggc cgcttttttt acctttgggc ttttgagccc gggttccaaa agggcgaatt
     1021 tcttggaaaa acaaggggcc ggtcccgccg aatggcgggg cttcggatgg accccaagga
     1081 gccatttcat gtcggaacag ccgatccagg tgaaccccca cgaaaacctg atgaccccct
     1141 ttgacgcggg
//
LOCUS       DASTJJ010000856         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_974914, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000856 DASTJJ010000000
VERSION     DASTJJ010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974914"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..940)
                     /locus_tag="VFR02_10335"
     CDS             complement(8..940)
                     /locus_tag="VFR02_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870876.1"
                     /translation="MVSGFFGTVIGLARARVLASKLGLLVPLATALGAWVLMTGEPEN
                     WGALLFWAGSLGYLLVSLGYFGRQPAPIAALALAGGCGWAWSNFFWWRGVPIPHLMGG
                     WQSLFVLTIASERLETVHWQGKGWDRWVLSLAVLTILAGVAYQPFQPGPGVRLLSAGY
                     LLFALCLLFLDRGWSGGDAKAWGSFTKACLVSAYTWLLIGAGVGSILAPVDSGFAYDA
                     FLHCVFLGLVFSMVFSHAPLLLPRVLRTPVPFHPVFYAHWILLQASLVVRVSGDLMGS
                     TPWRRWGALLNALALALFVINTWVAVIRGRRSRT"
     gene            complement(1187..>1648)
                     /locus_tag="VFR02_10340"
     CDS             complement(1187..>1648)
                     /locus_tag="VFR02_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9870877.1"
                     /translation="LNYAKVSPQSYRTMKAFSDFTKTTGLDKKLLELVKIRASQLNAC
                     AFCLDMHTKDARAMGENEERIFMLDAWRESGLYSDKERVALAWTEALTLLPKTHAPEA
                     DYRAVRDLFSEEETVHLTHGIVVINAWNRFMVAFRAPAGLYQPPVQDPKGT"
BASE COUNT          382 a          492 c          515 g          259 t
ORIGIN      
        1 gatcccctca agtcctggag cgtctcccac ggatcaccgc gacccacgta ttgatgacga
       61 atagggccag ggctagcgcg ttgagcaggg caccccagcg gcgccaaggg gtcgatccca
      121 tcaagtcgcc gctcacccta acgacgaggg aggcctgtag aaggatccaa tgggcataga
      181 agaccggatg gaacggaacg ggggtcctga ggacacgggg caggagaaga ggggcgtggc
      241 tgaagaccat cgagaacacg agccccagga aaacgcagtg taagaaagcg tcgtaggcaa
      301 acccggaatc caccggcgca agtatcgatc ccacccctgc gccgatcagg agccaggtat
      361 aggccgatac caggcaggcc ttggtgaagg acccccatgc tttggcatct cctcccgacc
      421 agccacggtc caggaacaac aggcaaaggg cgaaaaggag ataaccggcg gaaagcagcc
      481 ttacccccgg cccgggttgg aaaggctggt aggccacccc ggccaggatc gtgaggaccg
      541 ccaaggaaag gacccaccga tcccaaccct tgccctgcca atggaccgtt tccaagcgtt
      601 cggaggcgat ggtcagcacg aacagggatt gccatcctcc catgaggtgg gggatgggga
      661 cgccacgcca ccagaaaaaa ttggaccaag cccatccgca cccgcccgcc agggccaggg
      721 cggcgatcgg ggcgggctgc cggccgaagt aacccaggga gaccaaaaga tagcccaggc
      781 tcccggccca gaacagcagg gcgccccaat tttcgggctc gcctgtcatc aggacccagg
      841 ccccaagggc ggtcgccagc ggcaccagga gccccagttt ggaggccaag accctggccc
      901 gggccagccc gatgaccgtc ccgaaaaaac cgctgaccat caaggggcca tggaaggaga
      961 tccaattagc ctggggaatg ggaaggtgcc attggagccg taacagccca ccccatacgg
     1021 cgaagatcaa tgtcaggaat gctgaaaggg caagcggcaa cctatagggg acccgggaca
     1081 tccatgcctc cgttctgatc aatgaaaaag gccttagggg ccgggcacta ctatgggctt
     1141 gtctcccaag gtccgccgtg acttatctca cggccgtcac ggaaggtcag gttcccttgg
     1201 ggtcttggac cgggggttgg taaaggcccg ccggggcccg gaaggcgacc atgaagcggt
     1261 tccaggcgtt gatgaccacg atgccatgcg tgagatggac cgtctcttcc tcagagaaaa
     1321 ggtcgcggac cgcccgataa tcggcttccg gagcatgcgt tttcggaagg agggtcaaag
     1381 cttcggtcca agcgagggca accctttcct tgtcggaata aaggccggat tccctccagg
     1441 catccagcat gaatatccgt tcctcgtttt cacccatggc cctggcgtct ttcgtgtgca
     1501 tatccaggca gaaggcgcaa gcgttcagtt gggaggcccg gatcttgacc aattcgagga
     1561 gtttcttgtc gagcccggtc gtcttggtga aatcgctgaa ggccttcatg gtcctataac
     1621 tttgggggga cactttcgcg taattcag
//
LOCUS       DASTJJ010000857         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1706529, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000857 DASTJJ010000000
VERSION     DASTJJ010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706529"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFR02_10345"
     CDS             complement(<1..845)
                     /locus_tag="VFR02_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET9870878.1"
                     /translation="MRTLSLACLLALTLAGCAEVQPQDDPFALSRPPDRPLPQALSAE
                     GTRIRVAIVVHQPSVRLSAPDAFILTGLSLEGTPEADGTGKRLKDIELTPRQIDGPRA
                     VLTPQGQGEVEIDGKPYRGSIVILDNGDGTLTVVNDVDLEDYVMGVVAGEVPRNWPLE
                     ALKAQALAARTFAVLKREQAQQAGSPYDLENSSLFQMYKGSGFLSDGIRQAVQETQGE
                     IITYGGRPIEAFFHSNCGGETCGAKSVWGQDKPYLKPVVCGFCRDGAHFRWTAEMAIP
                     DLVRKL"
     gene            complement(842..1069)
                     /locus_tag="VFR02_10350"
     CDS             complement(842..1069)
                     /locus_tag="VFR02_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET9870879.1"
                     /translation="MSELSSLGKGLALTGLVLILLGGLLALAGKVPWIGRLPGDIRIE
                     KPGFSLYFPLGTCLLASVLLSSLAWLFSRLR"
     gene            complement(1138..1797)
                     /gene="fsa"
                     /locus_tag="VFR02_10355"
     CDS             complement(1138..1797)
                     /gene="fsa"
                     /locus_tag="VFR02_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET9870880.1"
                     /translation="MKFFLDTANLDEIKQGAAMGLVDGVTTNPSLVVKEQGVDFHTRI
                     KEICAIVNGSVSAEVTAVDFDGMMKEGHTLAKLHDNVTVKVPLIPEGLKACRALSQEG
                     IKVNVTLCFSPSQALLAAKAGATFISPFVGRLDDVSQDGMQLIKDIKLIYDNYGFKTQ
                     ILAASLRHPMHVVESAKAGAHVGTMPFSVLKMLFNHPLTDIGLKKFLADWEKTKEAQK
                     K"
     gene            complement(1871..>2339)
                     /locus_tag="VFR02_10360"
     CDS             complement(1871..>2339)
                     /locus_tag="VFR02_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9870881.1"
                     /translation="VPALKPGERRALLIVFGLGLFFFAAGVLFGYGVVIPLALRFLVG
                     YNLSFHFIPQWTLDGYFNFVMNFLLIFGLVFELPLVLAALVSIGVATPAFLAHKRRHA
                     IVGIFIAAAFLAPSADPVTQLIVALPLVVLYEAGIWLSYLAQRPPAPLPGPPR"
BASE COUNT          462 a          736 c          749 g          392 t
ORIGIN      
        1 agcttccgga ccaggtccgg gatggccatt tcggcggtcc agcggaaatg cgcgccgtcc
       61 cggcagaaac cgcagaccac gggcttcaag tagggcttgt cctggcccca gaccgacttg
      121 gcgccgcagg tctccccccc gcagttcgaa tggaagaagg cctcgatggg ccggccgcca
      181 taggtgatga tctcaccctg ggtctcctgg accgcctggc ggatgccgtc gctgaggaac
      241 cccgagccct tgtacatttg gaacagggag gaattttcca ggtcgtaggg acttccggcc
      301 tgttgggctt gctcccgctt caggaccgcg aaggtccggg ccgccagggc ctgggccttc
      361 agggcctcca agggccagtt acgcggcacc tcgcccgcca ccacccccat gacatagtct
      421 tccaggtcca cgtcgttgac caccgtcaag gtgccgtcgc cgttgtccag gatcacgatg
      481 gaaccccggt agggtttccc gtcgatctcc acctcccctt ggccttgggg cgtcaggacg
      541 gcccgggggc cgtcgatctg ccggggcgtc agttcgatgt ccttcaggcg cttccccgtg
      601 ccgtcggctt cgggggtccc ctccaagctc aggccggtca ggatgaaggc gtccggcgcc
      661 gacagacgaa cgctgggctg gtggaccacg atggccaccc ggatgcgggt gccctccgcc
      721 gacaaggcct ggggcagcgg ccgatcgggc ggccgggaca gggcaaaggg gtcgtcctgg
      781 ggttggacct cggcgcaacc cgccagggtg agggccaaca gacaagcgag ggaaagggtc
      841 ctcatcgaag cctggaaaaa agccaggcca gggaagacag caggaccgag gccagcaggc
      901 aggtccccag ggggaaatag agtgaaaagc ccggcttctc gatgcggata tccccgggca
      961 accggccgat ccaagggacc ttgcccgcca aggccaggag cccgcccagc aggatcagga
     1021 ccaggccggt cagcgccaga cccttcccca aggatgacag ttcggacacg cggtccccct
     1081 ccgggcaaaa aaaagggcgg cccatggacc gcccttccct gccctgctta agactggtta
     1141 ctttttctgg gcttccttgg tcttttccca atcggccagg aacttcttca ggccgatgtc
     1201 cgtcagggga tggttgaaga gcatcttgag cacgctgaag ggcatggtgc ccacgtgggc
     1261 gccggccttg gccgattcca ccacatgcat cggatggcgc aaggaagcgg ccaagatctg
     1321 ggtcttgaag ccgtaattgt cgtagatcag tttgatgtcc ttgatcagct gcatgccgtc
     1381 ctgggacacg tcgtccaaac ggcccacgaa ggggctgatg aaggtggcgc cggccttggc
     1441 cgccaagagc gcctgcgagg gcgaaaaaca gagggtgacg ttgaccttga tgccttcctg
     1501 gctgagcgcc cggcaggcct tcaggccctc ggggatgagc ggcaccttga cggtgacatt
     1561 gtcgtgcagt ttggccaggg tgtggccttc cttcatcatc ccgtcgaaat ccacggcggt
     1621 gacttccgcc gataccgacc cgttgacgat cgcgcaaatt tccttgatgc gggtatggaa
     1681 atcgacgcct tgttccttga ccaccagcga ggggttcgtc gtcaccccat cgaccaggcc
     1741 catggccgcg ccttgtttga tctcgtccag attggccgta tccaagaaga atttcatgcc
     1801 gtcctccacc tttccccacg gggggttgac tgtcgtcctg attaaattat ggggtttccc
     1861 aaatcaaaag tcaacgcggg ggccccggca ggggcgccgg ggggcgctgg gccaggtaag
     1921 aaagccagat cccggcctca tataggacca ccaggggcaa ggccacgatg agctgggtga
     1981 ccggatccgc gctgggcgcc agaaaagccg ccgcgatgaa gatgcccacg atggcgtgcc
     2041 gccgcttgtg ggccaaaaag gccggggtgg cgaccccgat gctgaccagg gccgccaaca
     2101 ccaggggcaa ttcgaacacc aacccgaaga tcaatagaaa gttcatgacg aaattgaaat
     2161 atccgtccag ggtccattgg gggatgaaat gaaagctcaa gttgtacccc accaagaacc
     2221 gcagggccag ggggatgacc accccgtaac cgaacagcac ccccgcggcg aaaaagaaca
     2281 accccaggcc gaagacgatc aagagggccc ggcgttcccc gggtttgagg gccggcacg
//
LOCUS       DASTJJ010000858         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1585936, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000858 DASTJJ010000000
VERSION     DASTJJ010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585936"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFR02_10365"
     CDS             <1..714
                     /locus_tag="VFR02_10365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9870882.1"
                     /translation="PGLRKYYDLSAPAGDEARVSPMELDARVLRLLGLEAPGYDRPGG
                     TWPVPEEARRAADAFWKAHGFGEKDQVVAVNPFASCPSKEWLPEKWAAVLKELLANGF
                     KAFFTCAPLEKKGLKAIEDRLGRSLPAYAGAPLVPLMGLYQRSRVVLSVDSGPRHLAA
                     AVGTPTLTVWGPEPLDRWHPYSRERHPVLVRDVDCRPCGLSVCVARKHECMVQLDPKG
                     LLKTLKALLRRTLAPGTVA"
     gene            714..1757
                     /locus_tag="VFR02_10370"
     CDS             714..1757
                     /locus_tag="VFR02_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9870883.1"
                     /translation="MAAPGRLLLIRPRFLGDLVLATGLPRAIKDQWPGTRVSLLAESA
                     YAEALRHHPDLDEVVEFDASRKGRPLYLLDFYRGLRRRRFDVVLDLFGNPRTAQMAFL
                     SGAPLRVGFDLRGRRWAYNRLAPPSSPPLASGRRPVTEAYLDQLRALGWKAQGPYRTS
                     LTVTGEEKAHARKLLDRARLFPGAKLAVLAPGASWPAKHWPLEKFARLGAYLSAEGFR
                     ILTVFGPKEKGMTEAFQGRMGKDWILIQEPSLRGLMAFIEAADLLVSNDAGPMHIGPA
                     VGTPTLGIFGPGEPEVWFPYAEPHRVLYAEVPCSHCGLDACPLMACMAALTPEAAARA
                     ALEMTASLPAKNR"
     gene            1854..>2631
                     /locus_tag="VFR02_10375"
     CDS             1854..>2631
                     /locus_tag="VFR02_10375"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9870884.1"
                     /translation="MFGKGKIWMDGKLVDWKKANIHVLSHAVHYGSCVFEGVRCYNTK
                     QGPGIFRLDAHVDRLFDSAKIYRMEIPYTKKQFADAIVKVVKVNKLKACYIRPFVFRG
                     YGAMGLYPLKNPLQCAVAAWSWGSYLGEEALSKGISVRISSWQRQAPNTFPALAKAAG
                     NYLNSQLVKMEAVQDGFDEGIALDHYGYVSEGSGENVFIVKNGVIFTPPTSGAILAGI
                     TRHSIFVLARDMGLEIRQQVLPRESLYIADEVFLTGTAAEM"
BASE COUNT          409 a          890 c          885 g          447 t
ORIGIN      
        1 ccgggcctgc gcaagtatta cgacctgtcg gccccggcgg gggatgaggc gcgggtctcc
       61 cccatggaac tggacgcccg ggtgctcagg ctcttgggcc tggaggcccc gggctatgac
      121 cgcccgggag gcacctggcc cgtgcccgag gaggcccgcc gggccgccga cgctttttgg
      181 aaggcccatg ggttcgggga gaaggaccag gtggtggcgg tgaacccctt cgccagctgc
      241 cccagcaagg agtggctgcc ggaaaaatgg gcggcggtcc tcaaggaact gctggccaac
      301 ggtttcaagg ccttcttcac ctgcgcgccc ctggaaaaaa aggggttgaa ggccatcgag
      361 gaccggttgg gccgaagcct gccggcctac gcgggggcgc cgttggtgcc cttgatgggg
      421 ctttaccaaa ggtccagggt ggtgctgagc gtggattcgg ggccccgtca cctggccgcc
      481 gccgtgggga ccccgaccct gacggtctgg ggccccgaac ccttggaccg atggcacccc
      541 tattcccggg aacggcaccc ggtgttggtg cgggacgtgg attgccggcc ctgcggtctc
      601 tcggtctgcg tggcgcgcaa gcacgagtgc atggtccagc tcgatcccaa gggcctgctc
      661 aagacgctga aggccctcct gcgccgcacc ctggcgcccg gcacggtggc ctgatggcgg
      721 cgcccggccg gctgctgttg atccgtcccc ggttcctggg ggacctggtg ctggccacgg
      781 gcctgccgcg cgccatcaag gaccaatggc cgggcacccg ggtgtccctg ctggccgaaa
      841 gcgcctacgc cgaagccctg cgccaccacc cggacctgga cgaggtggtg gagttcgacg
      901 cttcccggaa gggccgcccg ctctacttgt tggactttta ccggggcctg cgccgccgcc
      961 ggttcgacgt cgtgttggac cttttcggca atccccgcac ggcccaaatg gccttcctgt
     1021 cgggcgcgcc gctgcgcgtg gggttcgacc tgagggggag gcggtgggcc tacaaccggc
     1081 tggcgccgcc ttcctcgccg cccttggctt cgggccgccg ccccgtcacg gaggcctacc
     1141 tggaccagct gcgggccctg ggatggaaag cccaaggccc ctatcggacc tccttgacgg
     1201 tgaccggtga ggagaaagcc cacgcgcgca aattgctgga ccgggcccgg cttttcccgg
     1261 gggcgaagct cgcggtcttg gcgccgggcg cctcctggcc ggccaagcat tggccgttgg
     1321 agaaattcgc ccggctcggg gcctacctgt ccgccgaggg cttccgcatc ctcacggtct
     1381 tcggccccaa ggaaaagggg atgacggagg cgttccaggg ccggatgggc aaggactgga
     1441 tcctcatcca ggaaccttcc ctgcggggcc tgatggcctt catcgaggcg gcggacctct
     1501 tggtctccaa cgacgcgggg cccatgcata tcggtcccgc ggtgggcacg cccaccctgg
     1561 gcattttcgg acccggggaa cccgaggtct ggttccctta cgcggagccc caccgggtcc
     1621 tctacgccga agtgccctgc tcccactgcg gcctggacgc ctgccccctg atggcctgca
     1681 tggcggcctt gacccccgag gcggcggccc gggcggcgct ggaaatgacg gcgtcccttc
     1741 ccgccaaaaa ccgttgaaaa acaagggcaa gtcccttccc ccgaaatccc ctttgctcct
     1801 ataatgcagc ctcgccctgg ggctggattt aaacctgtat cgaggagttt cccgtgttcg
     1861 gcaaaggcaa gatctggatg gacggcaagc tggtcgactg gaaaaaggcg aatatccatg
     1921 tgctgtccca cgcggtccat tacggctcct gcgtcttcga gggcgtgcgc tgctacaaca
     1981 ccaagcaggg cccgggcatc ttccgcctgg acgcccacgt ggaccggctc ttcgattcgg
     2041 ccaagatcta ccgcatggag atcccctaca ccaagaaaca gttcgccgac gccatcgtga
     2101 aggtggtgaa ggtcaacaag ctcaaggcct gctacatccg gcccttcgtg ttccggggtt
     2161 acggggccat ggggctctat cccttgaaga accccctgca atgcgcggtg gcggcctggt
     2221 cctggggctc ctacctgggc gaggaagccc tgagcaaggg gatctcggtg cgcatctcct
     2281 cctggcagcg gcaggcgccc aataccttcc ccgcgctggc caaggccgcc ggcaactacc
     2341 tgaactccca attggtcaag atggaggccg tgcaggacgg gttcgacgag ggcatcgcgc
     2401 tggaccacta cgggtacgtg tcggaagggt cgggcgagaa cgtgttcatc gtcaagaacg
     2461 gcgtcatctt cacgccgccc accagcgggg ccatcctggc gggcatcacc cgccattcca
     2521 tcttcgtgct ggcccgggac atgggcctgg agatccgcca acaggtgctg ccccgggagt
     2581 ccctttatat cgccgacgag gtcttcctca cggggacggc cgccgagatg g
//
LOCUS       DASTJJ010000859         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1340755, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000859 DASTJJ010000000
VERSION     DASTJJ010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340755"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..551)
                     /locus_tag="VFR02_10380"
     CDS             complement(342..551)
                     /locus_tag="VFR02_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870885.1"
                     /translation="MKRFFAVLTTVAFLGTTAVVLADDATPVATPAAAAAKPMKKHHV
                     VKKHVMKKKEAPAATPAATTTPAAK"
     gene            complement(736..1671)
                     /locus_tag="VFR02_10385"
     CDS             complement(736..1671)
                     /locus_tag="VFR02_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9870886.1"
                     /translation="MRTVIGVDLGGTNIVAAVVDERGRVLGKDKRPTQAPLGAVGVMD
                     RIVESVRAASAASGLPWKSHLRLGIGSPGPLNPKKGIILYTPNLNWKNVHLTEYLGRK
                     TGKKVVLENDANLAALGETWIGAGRGEGFVLCVTLGTGVGGGFILDGKIYEGAWGVSN
                     HIGHVVVDPKGPRAPYGNRGILEQYVSATGIVRLAGLAGLKPPRGEKLEAHTFQKMAE
                     RGNAKALRVYDQAGTMLGVGLTSAIHLLNPGMIIFAGGVAAAGELLFKPMRRELKERC
                     FASHLKGLRFRTAKLGDNMGVVGAARLAWQSLGSK"
BASE COUNT          317 a          587 c          542 g          334 t
ORIGIN      
        1 gccgtccttg gtctggatct tcagcgagac gcccggcacc tcctcggtga tggtgccgtg
       61 gatgatggac ccgtccttca ggtacaccac atcctcggcc caaagccgtc cggcggcggc
      121 caaaaggaac agggccgaga aaaacaaggt taaacgtttc atgactcccc ccaaaaagat
      181 cccccgatgg gtcggcggct gactggtcgg ctcacgcggt gggaaaaatg gcgatgacaa
      241 cgggttcatt ttggccgtgg aggagggtct ttgtcaaact atttggccgt caaaaaaaaa
      301 ggccccgggc tttcgcccgg ggcccttgga ctgggcctga attacttggc ggccggagtc
      361 gtggtggcag cgggggtcgc ggccggggct tctttcttct tcatcacgtg cttcttgacc
      421 acatgatgct tcttcatcgg cttggcagcg gccgcagccg gggtcgcaac cggggtcgcg
      481 tcatcggcca gcaccaccgc ggtggtaccc aggaaagcaa cggtcgtcaa aacggcgaaa
      541 aaacgcttca tgaaacgcct ccttaagatg agaaggcggt tggcccgccc tctgttccaa
      601 ccccgggcct ctgcccggga gccggaggat attaaagaat tctttaatat tccacccatt
      661 ggacgggtca accccatccc tggttccagc atttcatcga aaaattggcc gggaaaccct
      721 tggaaaataa gggttttact tggacccgag ggactgccag gccaggcgcg cggcccccac
      781 cacccccatg ttgtcgccca gtttggcggt ccggaacctc aggcccttca ggtgggacgc
      841 gaaacaacgt tctttcagtt cccggcgcat gggcttgaag agcaattccc cggcggcggc
      901 gacccccccg gcgaagatga tcattcccgg gttcaagagg tggatggcgg aggtgagccc
      961 cacccccagc atggtccccg cctggtcata gaccctcaag gccttggcgt tgccccgctc
     1021 ggccatcttt tggaaggtat gggcctccag cttttcgccc cgggggggct tgagcccggc
     1081 caacccggcc agccgcacga tgccggtggc ggacacgtac tgctccagga tgccccggtt
     1141 gccgtagggg gcccgggggc ctttcgggtc caccaccacg tggccgatgt ggttgctcac
     1201 gccccaggcg ccctcataga tcttgccgtc gaggatgaag cctcccccca cccccgttcc
     1261 cagggtgacg cacaacacga agccttcccc ccgccccgcc ccgatccagg tctcccccag
     1321 ggcggccagg ttggcgtcgt tctccaacac caccttcttc ccggtcttgc ggcccaggta
     1381 ctcggtgagg tggacgttct tccaattgag gttgggggta tagaggatga tccccttttt
     1441 ggggttgagc ggtcccggcg acccgatgcc caggcgcagg tgcgacttcc aaggcagacc
     1501 gctggccgcg ctggcggccc ggacgctttc cacaatgcgg tccatcacgc ccaccgcgcc
     1561 caagggggct tgggtcgggc gcttgtcctt gcccaggacc cggccgcgct catccaccac
     1621 ggcggccacg atgttggtgc cccccagatc caccccgatg acggttctca cggaattcct
     1681 cccttggaaa aatcgggtta ttgtaacaag gcgccccgaa aaggaaagcg ctttcagccc
     1741 ctcagggccg gctgcggtag gcccggtggt ccatgccggg
//
LOCUS       DASTJJ010000860         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_241477, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000860 DASTJJ010000000
VERSION     DASTJJ010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241477"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFR02_10390"
     CDS             <1..270
                     /locus_tag="VFR02_10390"
                     /inference="COORDINATES: protein motif:HMM:NF036547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9870887.1"
                     /translation="ADSETIYPSNLPLDIQTFQKDEYLNVLKEDASLTEKVENYEKEL
                     ILKAMEKANHVQTKAAKLLDVNRTALIYKLKKYGLLSEGPETGEE"
     gene            267..1232
                     /locus_tag="VFR02_10395"
     CDS             267..1232
                     /locus_tag="VFR02_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870888.1"
                     /translation="MTERRSLYRALAVLWVCLPPLGAAAQEQPPSIQDLPASQGEAWT
                     RSIAGFETAVSAQARALADNRRRQERLGSEIAALETKTAALRDQASGNLFDQLRLKKL
                     LAELQGKLEAQAALKQDRDARLADFEQKGLSLLSLYNESISRQLDLPEGSEPGDRLDS
                     VIATVRKRQALQGLLDRFPGAPDDQRMAPLSLLDQSGGRDLASLKTTLDILQDREKEL
                     QTRIEKADVRAAELNQEITLQGKMRDFLSDMRKMNEASSFPRESLNRADLQALESQAQ
                     DSNLKRGIQAAQKRQDQDRADLEQVRRYIRSVQARIQEWKGGGSR"
     gene            1229..>2183
                     /locus_tag="VFR02_10400"
     CDS             1229..>2183
                     /locus_tag="VFR02_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870889.1"
                     /translation="MRTIMMAVWGWACLAPLAACAQVQWVFQPLGLETRFDDNVFRLV
                     SQAGRLSDEVDSLSGGLGLEARDGGFSAKGGYSLNADAYQFYSGLDNFTHDLTLDLSQ
                     ESGDFLLRYTNDSFFRSSAYYEFDYFDEANLVALEYDPDPGWSLRGGVEALSRDYYAD
                     QDYVVSRNFTDDGASISAQRLWPDSWSAKLALRYGERRYNRYAVASSGSGSVTAVGDL
                     QDDRTVSLDLGLHAYLLQVLQDLHLTCARTNSNSYGFSNWVGSLSWAGVLSPAKDFYL
                     ELFFRLYEKDYDFQPLDVPDLQLGFSNDDGQDLLAVKGSWEW"
BASE COUNT          401 a          717 c          719 g          346 t
ORIGIN      
        1 gcggactcgg agaccatcta tccctccaac ctgcccctgg acatccagac cttccagaag
       61 gacgagtacc tgaacgtgct caaggaggac gccagcctca ccgagaaggt ggagaactac
      121 gagaaggagc tcatcctgaa ggccatggaa aaggccaacc acgtgcagac caaggcggcc
      181 aagctgttgg acgtgaaccg cacggccctc atctacaagc tcaaaaaata cggcctgttg
      241 tccgaaggcc cggagaccgg ggaagaatga cggaacgccg ctccctttac cgcgccctgg
      301 ccgtcctttg ggtctgcctg ccccccttgg gggcggccgc ccaggaacag ccgccctcca
      361 tccaggacct gccggcttcc caaggcgagg cctggacgcg ctccatcgcg ggatttgaga
      421 cggccgtttc ggcccaggcc cgggccttgg cggacaaccg gcgccggcag gaacggctgg
      481 ggtcggagat cgcggccctg gagaccaaga ccgcggccct gcgggaccag gcctcgggca
      541 acctcttcga ccaactgcgc ctgaaaaaac tcctcgccga gctccagggc aaactggagg
      601 cgcaggcggc cctgaaacag gatcgggacg cccggttggc cgatttcgag cagaaggggc
      661 tttcgctttt atccctttat aacgagtcca tcagccgcca attggacctg ccggaggggt
      721 cggagccggg ggaccggctg gactcggtca tcgccaccgt gcgcaaacgc caggcccttc
      781 agggcctgtt ggaccggttc ccgggggccc cggacgacca gcgcatggcg ccgctctcgc
      841 tcttggacca gtccggcggc cgggacctgg ccagcctgaa gaccaccctg gacatcctcc
      901 aggaccggga aaaggaactc cagacccgta tcgagaaggc ggatgtccgg gccgccgagt
      961 tgaaccagga gatcaccctg cagggcaaaa tgcgggactt cttgtcggac atgcgcaaga
     1021 tgaacgaggc ttcgtccttc ccccgggaaa gcctgaaccg ggcggacctc caggccctgg
     1081 aaagccaggc ccaggactcg aatctcaaac gtgggatcca ggcggcgcag aagcggcagg
     1141 accaggaccg ggcggacctg gagcaggtgc gccggtacat ccggtcggtc caggcccgga
     1201 tccaagaatg gaaggggggt ggttcccgat gaggacgatc atgatggcgg tgtgggggtg
     1261 ggcctgcctg gcccccctgg cggcctgcgc gcaggtccag tgggtgttcc agcccctggg
     1321 gctggagacc cggttcgacg acaacgtctt ccgcctggtg tcccaggccg gacgcctgtc
     1381 cgacgaggtg gattcgctct ccggcgggtt ggggctggag gcccgggacg gcggattctc
     1441 ggccaagggc ggctacagcc tcaacgcgga cgcctaccaa ttctattcgg ggctggacaa
     1501 cttcacccac gacctgaccc tggacctttc ccaggaatcg ggggatttcc tgctgcgcta
     1561 caccaacgac tccttcttcc ggtccagcgc ctactatgag ttcgattatt tcgacgaggc
     1621 caacctggtg gcgttggaat acgacccgga cccggggtgg agcctgcggg ggggcgtgga
     1681 ggccctgtcc cgggactatt acgccgacca ggactacgtg gtgtcccgca acttcaccga
     1741 cgacggcgcc tcgatctcgg cccaacgcct ctggccggat tcctggagcg ccaagctggc
     1801 cctgcgctac ggggaacggc ggtacaaccg ctacgcggtg gcgtcctccg gatcggggtc
     1861 ggtgaccgcc gtcggggacc tgcaggacga ccgcacggtc tccctggacc tgggcctgca
     1921 cgcctacctg ctgcaggtgc tccaggacct ccacctgacc tgcgcgcgga ccaattccaa
     1981 cagctacggg ttctccaact gggtgggcag cctgtcctgg gcgggcgtgc tgagcccggc
     2041 caaggatttt tacctggagc ttttcttccg gctctatgag aaggactacg acttccagcc
     2101 gctggacgtg cccgacctgc aattgggctt cagcaacgac gacggccagg acctgctggc
     2161 cgtcaagggc agttgggaat ggg
//
LOCUS       DASTJJ010000861         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_241519, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000861 DASTJJ010000000
VERSION     DASTJJ010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241519"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFR02_10405"
     CDS             complement(<1..502)
                     /locus_tag="VFR02_10405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9870890.1"
                     /translation="MTAPARPLSLFWTTPGLPALLALLCWTAAPARGDDLSRALGKDF
                     NAGNFARVEESVQAALDRDPGQPRLWLELGALRQAEGDNAGALAAYQACGADSFPLRL
                     DEANTLLRLAKLDDAEALYQRLNAEKPDDARILWGLAQVDGDRARWDRFRTFAGRQAA
                     WARSQAW"
     gene            complement(513..1016)
                     /locus_tag="VFR02_10410"
     CDS             complement(513..1016)
                     /locus_tag="VFR02_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870891.1"
                     /translation="MSTTVHYFGKIRSPELLRELKDELQEIAQVSGWAYEKVDHLFTP
                     TDQAAAPARLTLKGIRLTLGKGLSPLQMTFDKDGYLSHIYYETVLTQNPLRPGVEKTT
                     QVLHQVHTSTVLKNADPQDHVRLVKLLDYVKKRYVPNLEVIDNTGYWYSRDESVLQGK
                     PLLLIPQ"
     gene            complement(1037..1339)
                     /locus_tag="VFR02_10415"
     CDS             complement(1037..1339)
                     /locus_tag="VFR02_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9870892.1"
                     /translation="MKLVLQDIVDEVSKAAGLAPPDAQVVARRILSCLEEGLFQGETI
                     EIRDFGVFKTRRVEGRVGRDLKRGTAIALPAHWKIKFKPGKRLKPVPVGGKQLSLV"
     gene            complement(1372..>1568)
                     /locus_tag="VFR02_10420"
     CDS             complement(1372..>1568)
                     /locus_tag="VFR02_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870893.1"
                     /translation="FVVFSDAVDYPASRALYEKEDPGVRVFCTALDGAVEVEIFPDGH
                     GRYRTFRRPDWVGFGPGGPS"
BASE COUNT          253 a          514 c          533 g          268 t
ORIGIN      
        1 gccaggcctg ggaacgggcc caggccgcct ggcggccggc gaaggtgcgg aagcggtccc
       61 accgggcgcg gtcgccgtcc acctgggcca ggccccagag gatgcgcgcg tcgtccggtt
      121 tttcggcgtt gagcctctgg tagagggcct ccgcgtcgtc caacttggcc aggcgcaaga
      181 gggtgttggc ctcgtccagc cgcaggggga aggaatcggc gccgcaggcc tggtaggccg
      241 ccaaggcgcc ggcgttgtcg ccttcggcct gcctgagggc gcccagctcc agccaaagcc
      301 ggggttggcc cgggtcccgg tccaacgcgg cctggaccga ctcctccacc cgcgcgaagt
      361 tccccgcgtt gaagtccttg cccagggcgc gggaaaggtc gtccccccgc gcgggcgcgg
      421 ccgtccagca caaaagcgcc agcagggccg gaaggcctgg ggtcgtccaa aaaagggaaa
      481 ggggccgggc gggggccgtc acgggaaagg cgttattggg ggatcagcag gaggggcttg
      541 ccctggagca cggactcgtc ccggctgtac caatagccgg tgttgtcgat gacctcgagg
      601 ttgggcacgt atctcttctt cacgtagtcc agcagcttga ccaaccggac gtggtcctgc
      661 gggtcggcgt tcttcaggac ggtgctggtg tgcacctggt gcaggacctg ggtggtcttt
      721 tccacgccgg gccgcagggg gttctgggtc agcacggtct cgtaatagat gtgggacagg
      781 tagccgtcct tgtcgaaggt catctgcagg gggctcaggc ccttgcccag ggtgaggcgg
      841 atgcccttca gggtcaggcg ggccggcgcc gcggcctggt cggtgggcgt gaagaggtgg
      901 tcgaccttct cgtaggccca accgctcacc tgggcgatct cctgcaactc gtccttgagc
      961 tcgcgcagga gctcgggcga tcggatcttg ccgaaataat ggaccgtcgt ggacattcct
     1021 tctccttaac gaacgactac accaggctca actgcttgcc gcccaccggc acgggcttga
     1081 gccttttgcc cggcttgaac ttgatcttcc aatgggccgg cagggcgatg gccgtgcccc
     1141 gtttcaggtc ccggcccacc cggccttcca cccgccgggt cttgaagacg ccgaaatcgc
     1201 ggatctcgat ggtttcgcct tggaacaggc cctcttccag gcaggacagg atgcgccggg
     1261 ccaccacttg ggcgtccggg ggcgccagcc ccgccgcctt ggacacttcg tccactatat
     1321 cttgtaggac gagcttcatg gaccccatta taggcagggt cccaacggat ttcaacttgg
     1381 cccccccggc ccgaagccca cccaatcagg acgccggaag gtgcggtaac ggccgtggcc
     1441 gtcggggaat atttccacct ccaccgcccc gtccaaggcc gtacagaaga cccggacacc
     1501 cggatcctcc ttctcataaa gggcccggga agcggggtaa tccaccgcgt cgctgaagac
     1561 gacgaaac
//
LOCUS       DASTJJ010000862         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1218173, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000862 DASTJJ010000000
VERSION     DASTJJ010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218173"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1310
                     /locus_tag="VFR02_10425"
     CDS             441..1310
                     /locus_tag="VFR02_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HET9870894.1"
                     /translation="MFQRLKDWSNLIVLSHSIFALPFALASLLVATHGHPSMRLTLLV
                     LLAMFFARTAGMANNRVMDADLDAQNPRTQGREIPAGKITKLQALILTWVAAAAFVAT
                     CYCIGGLAFKLSPLALGIVLFYSLTKRFTWWCHFFLGLALGLAPVGAWVAAKNALEPA
                     PLFLMGAVVFFVAGFDILYAIKDVDFDRAQGLKSWVVRWGIPASLRTALVLHFVLLLF
                     LAGFGVEADLSPLYIYGVAILGVFLTYQHTKIFAYARDRRGGDFILPIDVLGLNGWVS
                     VLVLAAVGVSLWF"
     gene            1301..2422
                     /gene="mqnE"
                     /locus_tag="VFR02_10430"
     CDS             1301..2422
                     /gene="mqnE"
                     /locus_tag="VFR02_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HET9870895.1"
                     /translation="MVLKKTLERSPIRDIYEKVEAGGRVTDAEALRLFETNDLYTLGA
                     MANLVRERKNGNHTYYNINRHIDYTNVCSTSCKFCAFSRFEGEAGAFEFTHQQIVQKA
                     KEAVEREGITELHIVGGLHPTYELEWYEEMLRLLKKHVPAVHLKCFTGVEINFFSDKY
                     NLSYREILTRLKAAGLGSMPGGGAEIFHPDVREEICPGKATADQWIEVHRTAHQLGLK
                     TNATMLYGHIEKYPHRVDHMRRLRELQDETGGFQTFIPLAFHPDHTAMDHFARPSGME
                     DLKTLAVGRVYLDNFPHIKAYWIMLGVRLAQLSLSYGVDDIDGTVIEEKIYHMAGADT
                     PEIMTVQELRRLIREAGRVPVERDTLYQELKPMPETAGV"
BASE COUNT          477 a          812 c          713 g          449 t
ORIGIN      
        1 ctatgaccac gcgggcatcc aggccgaggt ctcggcccgt tggcgcgagg cggactccga
       61 caaggccgac cgggaatcca ggaaataatc cggacataac ccggcttttt cccccgctct
      121 agccctctcc cgcaagggga gggggattaa gaacttctaa caaaaaccta tttcaccacc
      181 aagacaccaa gggcaccaag gaccgccaaa gctgaaagtc gaaaaatccg aataaattcg
      241 atcgcctttc ttggtgaact tggtgccttg gtggtaaaaa tggcctttat tttgattttt
      301 gttagaagct ccaaaataat atgaaaaaca aggccccggg ggtgaccgag gggtcggctt
      361 ttgcctataa tcatgccgct tcgccatcgg gcgaaagacg cttcggacag ggcccccgcc
      421 gccccattaa ggaaacccca atgttccaac gcctcaaaga ctggtccaac ctcatcgtgt
      481 tgtcccactc gatcttcgcc ctgcctttcg cgttggcgag cctcctggtg gccacccacg
      541 ggcatcccag catgcggctg accctgctgg tgctcctggc catgttcttc gcccgcacgg
      601 ccggcatggc caacaaccgg gtgatggacg ccgacctgga cgcccaaaat ccccggaccc
      661 agggccggga gatccccgcg ggcaagatca ccaagctcca ggcgctcatc ctgacctggg
      721 tggcggcggc ggccttcgtg gccacttgct attgcatcgg cggcctggcc ttcaagctct
      781 cccccctggc cctgggcatc gtcctcttct actccttgac caagcgcttc acctggtggt
      841 gccacttctt cctgggcctg gccttgggat tggcccccgt gggggcctgg gtcgcggcca
      901 agaacgcgct cgaacccgcg ccccttttcc tgatgggcgc ggtggtcttt ttcgtggcgg
      961 ggttcgacat cctctacgcc atcaaggatg ttgattttga ccgggcccag ggtctaaagt
     1021 cctgggtggt gcgttggggc atccccgcga gtttacggac ggccctggtc ctccatttcg
     1081 tcctgctgct cttcctggcg ggattcgggg tggaggcgga cttgagcccc ctctatatct
     1141 acggcgtcgc catcctgggg gtcttcctga cctaccaaca caccaaaatt ttcgcctatg
     1201 cgcgcgaccg gcgcggcggg gacttcatcc tgcccatcga cgtactgggc ctgaacggct
     1261 gggtctcggt gctggtcctg gccgccgtgg gagtgagcct gtggttctga agaaaaccct
     1321 cgaacggtcg cccatccggg acatctacga gaaggtggag gcgggcgggc gcgtcaccga
     1381 cgccgaggcc ctgcggcttt tcgagaccaa cgacctctat accctgggcg ccatggccaa
     1441 cctggtccgg gaacgcaaga acggcaacca cacctactac aacatcaacc gccacatcga
     1501 ctacaccaac gtctgctcca ccagctgcaa gttctgcgcc ttttcccggt tcgaggggga
     1561 agcgggcgcc ttcgagttca cccaccaaca gatcgtgcag aaggccaagg aggccgtcga
     1621 gcgcgagggc atcaccgagc tccacatcgt ggggggcctg caccccacct atgagctgga
     1681 atggtacgag gagatgctgc ggctgctgaa aaagcacgta cccgcggtgc acctcaaatg
     1741 cttcaccggg gtggagatca acttcttttc ggacaagtac aacctcagct accgggagat
     1801 cctcacccgc ctgaaagcgg ccggcttggg ttccatgccc ggcggcgggg ccgagatctt
     1861 ccatcccgac gtgcgcgagg agatctgccc cggcaaggcc accgcggacc agtggatcga
     1921 ggtgcaccgc accgcccacc aactggggct caagaccaac gccaccatgc tctacggcca
     1981 catcgagaaa tacccgcacc gcgtggacca catgcggcgc ctgagggaac tgcaggacga
     2041 gacgggcggc ttccagacct tcatccccct ggccttccac cccgaccaca ccgccatgga
     2101 ccatttcgcc aggcccagcg gcatggagga cctcaagacc ctggccgtgg gccgggtcta
     2161 cctggacaat ttcccgcaca tcaaggccta ttggatcatg ctgggcgtgc gcctggccca
     2221 attgtcgctt tcctacggcg tggacgacat cgacggcacg gtcatcgagg agaagatcta
     2281 ccacatggcg ggcgccgaca cgcccgagat catgacggtg caggagctgc gccggctcat
     2341 ccgcgaggcg ggccgggtgc ccgtggaacg ggataccctg taccaggaac tcaaacccat
     2401 gcccgaaacg gcgggcgtgt agataggcgt tcaggaaata tcctcaccac c
//
LOCUS       DASTJJ010000863         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_241695, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000863 DASTJJ010000000
VERSION     DASTJJ010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241695"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFR02_10435"
     CDS             complement(<1..416)
                     /locus_tag="VFR02_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870896.1"
                     /translation="MEMGNTAAPATIELTLSRAEELALEGNPLIHAADDRARAADRQA
                     SQSLSPADPTLMFESDDAGFRGPMAQQMLSLGEDLGFPGRSIAQADVDGAEAKRQAAL
                     AQDTRRMIVLQARQAYWDFYFRQKVSGVLEDAQRTW"
     gene            complement(591..944)
                     /locus_tag="VFR02_10440"
     CDS             complement(591..944)
                     /locus_tag="VFR02_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870897.1"
                     /translation="MRRFLKSLNRMFLAAGLLVALTPCGLCHAAGPSAGMSACAMKAS
                     DCGKDCCHSKQAPAACKAMDQSTLPATALSMKAPAAVAAPWTAPFRTACLAAGTPDRD
                     LFGIPLPRAPLSLRI"
BASE COUNT          211 a          342 c          380 g          196 t
ORIGIN      
        1 ttccaggtcc tctgggcgtc ttccaatacc cctgagactt tttgccggaa ataaaaatcc
       61 caataggctt ggcgagcctg gaggacgatc atccgccggg tatcctgcgc cagggcggcc
      121 tgccgttttg cttcggcccc gtccacgtcg gcttgggcga tactgcgccc cggaaaaccc
      181 aggtcttccc ccagggaaag catttgttgg gccatgggac cgcggaaccc cgcgtcatcc
      241 gactcgaaca tgagggtggg atcggcgggc gacaggcttt gggacgcctg ccggtcggcc
      301 gcccgggcgc ggtcatcggc ggcatggatg agtgggttcc cttccaaggc caattcctcg
      361 gccctggaca gggtcaactc gatggtggcc ggggcggcgg tgttccccat ttccatcccg
      421 gccatttcgc cggctttcgc cgggacatcg gcccctgtga cggttaaagc aaggcaggac
      481 aaaagtaata ttgagaactt cacgggcttc actccttttc agttcgcggg caaaaagaaa
      541 cctaggacgg gtccggtcgg actcgtccaa gcgaaactaa aaaggttgga tcagatcctt
      601 aaagagaggg gggctcgggg aagggggatc ccgaaaaggt cccggtcggg cgtgcccgcc
      661 gcgaggcagg cggtccggaa gggggccgtc cagggggctg cgacggcggc cggcgccttc
      721 atggaaaggg ccgtggccgg aagggtggac tggtccatgg ccttgcaggc cgcgggggcc
      781 tgcttggaat ggcagcagtc cttcccgcag tcggacgcct tcatggcgca agcggacatc
      841 ccggccgagg ggccggccgc gtggcacagg ccgcagggcg tcaaggccac caacaatccg
      901 gcggccagga acatccggtt caaggacttc aagaagcgtc gcatggatgg agtttaaccc
      961 tctttcgaaa ggacgtcaag tgaccccccc tattacggga aaagggggcg ggaagtttcc
     1021 cgtccatggt cagcgtaacc gcccccggga aggcccccta tgacgggcgt cataggggtc
     1081 agtgggcttc cagccagttc ttgcccacgc ccacgtccac cttcagggg
//
LOCUS       DASTJJ010000864         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1586315, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000864 DASTJJ010000000
VERSION     DASTJJ010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586315"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1015
                     /locus_tag="VFR02_10445"
     CDS             <1..>1015
                     /locus_tag="VFR02_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET9870898.1"
                     /translation="NNVPLDLGGRYLYVNDGGKVWNPGWKPVKAALDQYECRHGLGYT
                     KILGAKDGVEVEMLFFVPPGENLEIWKTRVKNTGKTPKDLKLYSYVEFCLFEALNDMT
                     NYQRTYSIGEVEVEGSAIYHKTEYRERRSHYALFACTKDVAGFDTSRDAFVGVHNGLH
                     EAAVPFAGKSSKSLAHGWNPIGSHELHLKLAPGQSEEFSFLLAYVDQGELPKFDKPFV
                     MNKAKGRELLAKYQRPGAVDAAFQAIQGMWRDLLSKFQVECPDPHATRMLNTWNQYQC
                     MATFNLSRSASLYETGIGRGMGFRDSNQDLLGFVHLIPERARQRILDIAATQLSDGTC
                     YHQ"
BASE COUNT          207 a          327 c          315 g          166 t
ORIGIN      
        1 ataataacgt gcccctggac ctgggcggcc gctatttata tgtgaatgac gggggcaagg
       61 tctggaaccc gggctggaag ccggtgaagg cggccctgga ccaatacgag tgccgccacg
      121 gcctgggcta taccaagatc ctgggggcca aggacggcgt ggaagtcgag atgctcttct
      181 tcgtgccgcc gggcgagaac ctggagatct ggaagaccag ggtcaagaac accgggaaga
      241 cgcccaagga cctcaaactc tattcctacg tggagttctg ccttttcgag gccctgaacg
      301 acatgaccaa ctaccagcgc acctattcga tcggggaggt ggaggtcgag ggatcggcca
      361 tctaccacaa gaccgaatac cgggagcgcc gcagccatta cgccctcttc gcctgcacga
      421 aggacgtggc gggtttcgac acctcccggg acgccttcgt gggggtccac aacggcctgc
      481 atgaggccgc cgtgcccttc gcggggaagt cgtccaagag cctggcccac ggctggaacc
      541 ccatcgggtc ccacgagctg cacttgaagc tggcgccggg gcaaagcgag gaattctcct
      601 tcctgctggc ctatgtggac cagggggagc tcccaaaatt cgacaagccc ttcgtcatga
      661 acaaggccaa gggccgggaa ctgctggcca agtaccagcg gccgggcgcg gtggacgcgg
      721 ccttccaggc catccagggg atgtggcggg acctcttgtc caagttccag gtggaatgcc
      781 cggaccccca cgccacgcgc atgctcaaca cctggaacca ataccaatgc atggccacct
      841 tcaacctgtc gcgctcggcc agcctttatg agaccggcat cggccggggc atgggcttcc
      901 gggattccaa ccaggacctg ctgggcttcg tgcacctgat ccccgaaagg gcccggcagc
      961 gcatcctgga catcgcggcc acccagcttt ccgacggcac ctgctaccac cagta
//
LOCUS       DASTJJ010000865         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_241953, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000865 DASTJJ010000000
VERSION     DASTJJ010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241953"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFR02_10450"
     CDS             complement(<1..584)
                     /locus_tag="VFR02_10450"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET9870899.1"
                     /translation="MNQQFKSLAFWLLAFVSILYFVNTFKNPGKKSESWDYSKFVTQV
                     QAGAVKKVEIQPDERLILGTAKDGSDFSAVEPDDPNLVKMLLDKNVQLEVEPPKRNDW
                     LQIFVSFGPIVLFLVVFYLMMRQSQGGGGGAMAFGKSRARLMTSGQQKVTFQDVAGVE
                     EAKTELMEVVDFLKDPKKYQSLGGKIPKGVLLVGP"
     gene            complement(680..>1767)
                     /gene="tilS"
                     /locus_tag="VFR02_10455"
     CDS             complement(680..>1767)
                     /gene="tilS"
                     /locus_tag="VFR02_10455"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET9870900.1"
                     /translation="FESLPRYHFFPGISFEAPMKHPAPSPLVPTPPFPKRARLLLALS
                     GGGDSTALLSLLADLRPPGTLFAAHVNYGLRGGASRADEAFVRALCRRFGVPLTVLRL
                     KDARARARREGKSLQDWAREKRYAFFARLCVKRGAWGVAAAHQADDQAETILDRILRG
                     AGTRGLTGLRPVQALRFGARGTGVRVWRPLLAHAREDLRRHLRSRGIRWREDAGNRKP
                     DYRRNRIRHQLLPLLARWNPSIARALVRMGEVASAQEDFLAQCLGPLRGRLGSRWGAS
                     SYRGRQRQWAREPLALKRLWVRTAAERLCPAARGLSFERVEEAREVWDGTRRGPRDLG
                     QGLSVEARGGVIWMRSNRLSPRGGRVK"
BASE COUNT          307 a          615 c          589 g          256 t
ORIGIN      
        1 ggccccacca gcagcacgcc tttggggatc ttgccgccca gggactggta cttcttcggg
       61 tccttgagga aatccaccac ctccatcagc tcggtcttgg cctcctccac gcccgccacg
      121 tcctggaagg tcaccttttg ctggcccgag gtcatcaggc gggcccggct cttgccgaag
      181 gccatggccc ccccgccgcc gccctggctt tgacgcatca tcaggtagaa gaccaccagg
      241 aaaagcacga tggggccgaa gctcacgaag atctgcagcc agtcgttgcg cttggggggc
      301 tcgacctcca actgcacgtt cttgtccaac aacatcttga ccaggttggg gtcgtcgggt
      361 tccacggcgg aaaagtcgct tccgtccttg gccgtaccca ggatgaggcg ctcgtcgggc
      421 tggatctcca ccttcttcac ggcgccggcc tgcacctggg tcacgaattt ggagtaatcc
      481 cagctctcgg acttcttgcc ggggttcttg aaggtattga cgaaataaag gatggacacg
      541 aaggccagca gccagaaggc cagggatttg aattgctggt tcacggaagg ctcctctcga
      601 atgaagacgc cccatgaata tccgtccccg ggggtgtttt ttcaaccctg aactgccccg
      661 gggagcggcc caaaagggcc tatttcacgc gcccgcccct gggggaaagg cggttggaac
      721 gcatccagat cacgcccccg cgtgcctcca ccgaaaggcc ctggcccagg tcccggggcc
      781 cgcgccgggt cccgtcccag acctcccgcg cttcttccac gcgctcaaag gaaagcccgc
      841 gggccgcggg acaaagccgc tcggccgccg tcctcaccca aagccgcttc agggccaggg
      901 gctcccgcgc ccattgccgc tggcgtcccc gataggacga ggcgccccaa cggcttccca
      961 accgcccccg caggggcccc aggcattggg ccaaaaaatc ctcctgggcc gaggccacct
     1021 cgcccatccg gaccagcgcg cgggcgatgg acgggttcca acgggcgagc aagggcaaga
     1081 gttggtggcg gatgcggttg cggcggtagt cgggcttgcg gttcccggcg tcctcccgcc
     1141 agcggatccc ccgcgagcgc aggtggcggc gcaggtcttc ccgggcatgc gccaagagcg
     1201 ggcgccagac ccggacgccc gtcccccggg cgccgaaccg cagcgcctgg accggccgca
     1261 gaccggtcaa cccccgggtc ccggccccgc gcaggatgcg gtccaggatg gtctcggcct
     1321 ggtcgtcggc ctgatgggcc gccgccacgc cccaggcccc gcgcttcacg caaaggcggg
     1381 cgaaaaaagc gtagcgtttc tcccgcgccc agtcctgcag cgacttccct tcccgccggg
     1441 cccgggcgcg ggcgtccttc aaccgcagga ccgtgagcgg aacgccgaag cggcggcaga
     1501 gggcgcgcac gaaggcctcg tcggcccggg aagcgccgcc gcgcaaccca tagttcacat
     1561 gggcggcgaa aagcgtgccc ggcggccgaa ggtcggccaa gagggagagc aaggcggtgg
     1621 aatcgccccc gcccgagagg gccaggagca gccgcgcgcg cttggggaaa ggcggcgttg
     1681 ggacgagggg ggacggcgca gggtgcttca tgggagcctc aaaggatatg ccggggaaaa
     1741 aatggtagcg ggggagagac tcgaact
//
LOCUS       DASTJJ010000866         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_976061, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000866 DASTJJ010000000
VERSION     DASTJJ010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976061"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..473)
                     /locus_tag="VFR02_10460"
     CDS             complement(45..473)
                     /locus_tag="VFR02_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9870901.1"
                     /translation="MQSRLRARSKLLSDINMVPFTDLVLVLLVIFMVTTPMLFQGHFK
                     VDLPQVAAPTPNLPEVTTVTITEGDKIYLDDKPVASEDLEAALKALSTSRPDALVMIN
                     ADKNVSHGTVTGVLSQAYLAGITKVGIAVDAPKPSAPAAP"
     gene            complement(483..>1098)
                     /locus_tag="VFR02_10465"
     CDS             complement(483..>1098)
                     /locus_tag="VFR02_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9870902.1"
                     /translation="MLQGTMLVPMLAKGGWVIAFLILLSVVVFAVWYDRWSFYRKNLA
                     PLGRVWPLLEPSVDAGSWKEALEACVREGTFLSQVAASGLRAKLSKQDPGPAMEREAK
                     VRLMALESRLPMLATIAGVAPFIGLFGTVLGIINAFKDLAAANAGGAGVVSEGIAEAL
                     VATASGLFVAITAVFIFNAHQARLNEAALEAEVVISQMTEKLES"
BASE COUNT          185 a          367 c          375 g          171 t
ORIGIN      
        1 aggccagcgg ccgggtcatg gggcggttct ccacgcgggg cctcctaggg ggcggccggg
       61 gccgacggct tgggcgcgtc caccgcgatg cccaccttgg tgatgcccgc caggtaggcc
      121 tgcgacagca cgcccgtgac cgtcccgtgg gaaacgttct tgtcggcgtt gatcatgacc
      181 agggcgtccg gccgggaggt cgaaagcgcc ttcagggccg cctccaggtc ctcggaggcc
      241 accggtttgt cgtccaggta gatcttgtcc ccctcggtga tggtcaccgt ggtgacctcc
      301 ggcaggttgg gcgtgggcgc ggccacctgg ggcaggtcga ccttgaaatg cccctggaac
      361 aacatgggcg tggtcaccat gaagatgacc aacaacacca gcaccaggtc cgtgaagggg
      421 accatgttga tgtccgaaag cagcttcgat ctggcgcgca ggcggctttg catggggtcc
      481 ccctaggatt ccagcttttc ggtcatctgc gaaatgacca cctcggcctc cagggccgcc
      541 tcgttcaacc gggcctggtg ggcgttgaag atgaagaccg ccgtgatggc cacgaagagc
      601 ccgctggcgg tggcgaccaa ggcctccgcg atcccctccg agaccacccc cgccccgccc
      661 gcgttggccg ccgccaggtc cttgaaggcg ttgatgatgc ccagcacggt gccgaagagg
      721 ccgatgaagg gcgccacgcc cgcgatggtg gccagcatgg gcagccggga ttccagggcc
      781 atcagccgga ccttggcttc gcgttccatg gcgggcccgg ggtcctgctt ggacagcttg
      841 gcccggaggc ccgaggccgc cacctgggac aggaaggtgc cttcccggac gcaggcctcc
      901 agggcctcct tccaggagcc cgcgtccacc gagggctcca gcaggggcca gacccggccg
      961 aggggcgcca ggttcttgcg gtagaagctc caacggtcgt accagacggc gaagaccacc
     1021 accgacagca ggatcaaaaa ggcgatgacc catccgccct tggccaacat ggggaccagc
     1081 atggttcctt gaagcatc
//
LOCUS       DASTJJ010000867         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_851028, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000867 DASTJJ010000000
VERSION     DASTJJ010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851028"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFR02_10470"
     CDS             complement(<1..760)
                     /locus_tag="VFR02_10470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9870903.1"
                     /translation="MRAPSGPGDGAIQAVDLARTLAILPVLALHATARLLPPGQALPG
                     LWDHLQRNGAYGVSLFFMISGFLITRTLDRGRDGAFQARWGAFYARRAGRILPLLALD
                     LLLGLALWRCFQNDPPGFGYSLNLPLNAADPGFWLPLLFFAFNWARALWALNDWGSIG
                     VHWAVLWSLAVEEQFYLVYPLVLRALGRVRNLVLFLVFLLAAGFGWRWFVYLQPLNNG
                     LDWKWGSFGYFDQLGLGIGLYFAQKEWGGRLAGRP"
     gene            complement(763..1425)
                     /locus_tag="VFR02_10475"
     CDS             complement(763..1425)
                     /locus_tag="VFR02_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870904.1"
                     /translation="MKRHLRNIAWETWVFTRRNWFLVVLMAFSSWIYERDHTELVAGL
                     SLTYIASFFFYVLTIYYPDRRNQRNMSRVVVPYLKEIVTQVKQVFDLARAGSTHACSL
                     ENPTDDDFFAVFGKIRPGDGSARLGFIGFDRWHDYLQHQRGRIERNLDKILVYEHFLE
                     TDFILELEKVRNSAFLEGLALADRQPDKHRDYAHLADPYFQCFRQMAALERQIKILSY
                     NF"
     gene            complement(1422..1871)
                     /gene="rimI"
                     /locus_tag="VFR02_10480"
     CDS             complement(1422..1871)
                     /gene="rimI"
                     /locus_tag="VFR02_10480"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET9870905.1"
                     /translation="MIFRPMLEVDLDQVVAIERASMPSPWSRELFEEELRRPLAHYFV
                     MEVEGQVAGYMGYWQAPEEAHIINLAVAPEFRRRGLGREMIERCVDYAAGRGSRLATL
                     EVREGNQAAQALYEKCQFRSIAIRKQYYSDNLEDAIVMIREMPEPDR"
     gene            complement(2084..2767)
                     /gene="tsaB"
                     /locus_tag="VFR02_10485"
     CDS             complement(2084..2767)
                     /gene="tsaB"
                     /locus_tag="VFR02_10485"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9870906.1"
                     /translation="MLAAIEVSTRICSVALLDLVTGEELAEVHLPPDWESSVTLIPAM
                     ESLLTQKGVGPQDLAAVAVSVGPGSFTGIRVGIATAEGLCLPSNLLAFGVPTLDGLAE
                     NLRRAELQGEAVALIDAHRGECFVGHYQIDPEEVQALEPPRILKPEQLVGEMKGRGWV
                     VGPGALKYEREIREGLGAQGVFAFTALHQPSAMSVAKLAYRRWQAGERPTLESLKPIY
                     LRPPSVDEK"
     gene            complement(2752..>3233)
                     /gene="tsaE"
                     /locus_tag="VFR02_10490"
     CDS             complement(2752..>3233)
                     /gene="tsaE"
                     /locus_tag="VFR02_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9870907.1"
                     /translation="DSLLTGSFLIDSPDETFQLGKLVGSGAGPNEVWCLTGPLGAGKT
                     HFVKGLAAGLGFEGPVTSPTFNLQHVYPARVPLYHFDWYRLEKPAEVEDLGWREWVEK
                     GGVTVVEGGDKFPQLLPERVLKAGLELLEGERRRVILEAVHPDIQGRLGEILKCWPL"
BASE COUNT          602 a         1059 c         1055 g          517 t
ORIGIN      
        1 aggggcgccc cgcgagccgg ccgccccatt ccttctgggc gaaataaagc ccgatgccca
       61 ggcccaactg gtcgaaataa ccgaaggacc cccatttcca atccagcccg ttgttgaggg
      121 gttggaggta gacgaaccac cgccaaccga acccggccgc cagcaggaag acgaggaaaa
      181 ggaccaggtt ccgcacgcgg ccgagcgcgc gcaggaccag ggggtaaaca aggtagaact
      241 gttcctcgac cgccagcgac caaagcacgg cccaatgcac gccgatggat ccccaatcgt
      301 tcagggccca gagggcccgg gcccagttga aggcgaagaa gagcaggggc agccaaaaac
      361 ccggatcggc ggcgttcaaa ggcaggttga ggctgtagcc gaaccccggc gggtcgttct
      421 ggaagcaacg ccaaagcgcc aggcccagca gcaggtcaag cgccaggagg ggcaggatgc
      481 gccccgcccg gcgggcgtaa aaagcgcccc accgggcctg gaaggcgccg tcccggcccc
      541 ggtccaacgt gcgggtgatg aggaaccccg agatcatgaa gaagagcgag accccgtagg
      601 cgccgttgcg ctgcaggtgg tcccacaggc cgggcagggc ttggcccggg ggcagcaggc
      661 gggcggtggc atgcagcgcc agcacgggca ggatggccag cgtgcgggcc aggtcgacgg
      721 cttggatcgc cccgtccccg gggccggaag gtgcgcgcat ggtcagaagt tataggagag
      781 gatcttgatc tgccgctcca gcgccgccat ctgccggaaa cactggaaat aggggtcggc
      841 caggtgggcg tagtcccggt gcttgtcggg ctggcggtcg gccagggcca ggccctccaa
      901 aaaagccgag ttgcgcactt tttccagttc gaggatgaag tcggtctcca ggaaatgctc
      961 atagaccagg atcttgtcca ggttgcgctc gatgcgcccg cgctggtgct gcaggtagtc
     1021 gtgccagcgg tcaaagccga tgaaccccag ccgggcggac ccgtcccccg gccggatctt
     1081 gccgaagacc gcgaaaaagt cgtcgtcggt ggggttctcc aggctgcagg cgtgggtcga
     1141 ccccgcccgg gccaggtcga agacctgctt gacctgggtg acgatctcct tcaggtaggg
     1201 caccaccacc cggctcatgt tgcgctggtt gcgccggtcc gggtaataaa tggtcaatac
     1261 gtagaagaaa aaggacgcga tgtaggtgag cgacaggccc gccaccagtt cggtatggtc
     1321 ccgctcgtag atccaggacg agaaggccat caggacgacc aggaaccagt tgcggcgggt
     1381 gaagacccag gtctcccagg cgatgttgcg caggtgcctt ttcatcggtc cggctcgggc
     1441 atttcccgga tcatgacgat ggcgtcctcc aggttgtcgc tgtagtactg tttgcggatg
     1501 gcgatggaac ggaattggca cttctcatag agcgcctggg cggcctggtt gccttcccgc
     1561 acctccaggg tggccaggcg ggagccgcgc cccgcggcgt aatccacgca gcgttcgatc
     1621 atttcccggc ccagcccgcg ccgcctgaac tcgggcgcga ccgccaggtt gatgatgtgg
     1681 gcctcctcgg gcgcctgcca atagcccatg tagcccgcca cctggccctc cacctccatg
     1741 acgaaataat gggccagggg gcgccggagc tcctcctcga acagttcccg ggaccagggc
     1801 gagggcatgg aggcccgctc gatggcgacc acctggtcca ggtccacttc caacatgggt
     1861 ctaaagatca tggggaacac ttccgacgaa aacacctgga ttttcaccac caagacacca
     1921 aggcaccaag gcacctcccc actttttaaa atcgaaccta tcgcaaaata ttacgtcgat
     1981 ccaccctcat taaacgagat cctcgaacaa gcgtttcttt ttccaaccga tcaaaaaagg
     2041 ccttacttgg tgccttggtg ccttggtggt gaccatgggc tttttatttc tcgtccaccg
     2101 agggcgggcg caggtagatg ggcttcaggc tttccagggt ggggcgctcg cccgcctgcc
     2161 agcggcggta ggccagtttc gccacgctca tggcgctggg ctggtgcagg gccgtgaagg
     2221 cgaagacgcc ctgggcgccc aggccctcgc ggatctcgcg ctcgtatttc agggcccccg
     2281 gccccacgac ccatccccgg cccttcatct cgcccaccaa ttgctcgggt ttgaggatgc
     2341 ggggcggctc cagggcctgg acctcctcgg gatcgatctg gtaatggccc acgaagcatt
     2401 cgccccgatg ggcgtcgatg agggccacgg cctcgccctg cagttcggcg cggcgcaggt
     2461 tctccgccag gccgtccagg gtgggcacgc cgaaggccaa aaggttcgag ggcagacaga
     2521 gcccctcggc ggtggcgatg cccacccgga tgcccgtgaa gctgcccggg cccaccgaca
     2581 cggccacggc ggccaggtcc tggggcccca cgcccttttg ggtgagcagc gactccatgg
     2641 cggggatgag ggtgaccgag ctttcccagt ccgggggcag gtgcacctcg gccaattcct
     2701 cgccggtgac caggtcgagc agcgccacgc tgcagatgcg ggtggagacc tctatagcgg
     2761 ccagcatttt aagatctccc ccaggcggcc ctggatgtcc gggtggacgg cctccaggat
     2821 gacgcgcctt ctttcgccct ccagcagttc caagcccgcc ttcaagaccc gctcgggcag
     2881 cagttggggg aacttgtcgc ccccttccac cacggtcacg ccgccctttt ccacccactc
     2941 ccgccagccc aggtcctcga cctcggcggg cttttccagg cggtaccagt cgaaatgata
     3001 gaggggcacc cgggccgggt acacgtgctg caggttgaag gtgggcgagg tgaccgggcc
     3061 ctcaaagccc agccccgccg ccaggccctt cacgaaatgg gtcttgcccg cgcccagcgg
     3121 gcccgtcagg caccagacct cgttggggcc cgcgccggaa cccaccagct tgcccagctg
     3181 gaaggtctcg tccgggctgt cgatgaggaa actgccggtc agcaaagagt cat
//
LOCUS       DASTJJ010000868         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1341896, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000868 DASTJJ010000000
VERSION     DASTJJ010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341896"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..1289
                     /locus_tag="VFR02_10495"
     CDS             648..1289
                     /locus_tag="VFR02_10495"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9870908.1"
                     /translation="MSLRLGDTAPDFTQDSSEGPIQFHAWLGDSWGVLFSHPADFTPV
                     CTTELGATAKLKDEFAKRGVKVLALSVDSVDSHKKWIPDINETQGTTVNFPILADADK
                     KVATLYDMLHPASTANVTVRSVFIIDNQKKIRATITYPASTGRNFNEILRVIDSLQLT
                     DKYKVATPVNWKDGDDVVIVPAVQDPKEIAERFPKGYKAVKPYLRLTPQPDKK"
     gene            1393..>1508
                     /locus_tag="VFR02_10500"
     CDS             1393..>1508
                     /locus_tag="VFR02_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET9870909.1"
                     /translation="MNVFPLMAGALLVMVIAYRYYSAFLAAKVVALDDRRPTP"
BASE COUNT          306 a          507 c          417 g          278 t
ORIGIN      
        1 gatgggcaac gaggccgaca cgggctactt cggcgagggg gaattcttgg agcgcatgca
       61 ccgcatcaac cagggccggc tcatctaccc cgcgcccgac aagctcaccc agtacgtgct
      121 caccgactac ctggacggca aacgcaagat catccagatc tgagcccttg tgttcctcgc
      181 cgtcatcgcg aggagcgcct ttcttcggtc ctgacgacga agcgatcccg atctatgacg
      241 atggtcacgt tttgacgccg tttttcgatc cttgaactgg gacaacggat cccatgggcc
      301 gagattgctt cgtcacaaga tccgttccgg agcgaaaacc acaacgctcc cctagaaccg
      361 acgcaatgac gacccagaac cgggccttga ctttgccgcc cgcccgggct taataatggg
      421 cttacggcga ggctttaacc cgggaccctg aaaagtcccc ccgtccccta caaaagggcg
      481 gacaacttgc cccggccgtt ccttcccgct tgaccgggga gggataaaca gggcgctaaa
      541 gcggtgaatg agtccgaaaa cccttcgaac ccgcgccgct tgaaaagccc gcgggttttt
      601 gtttttcccc cggtcctttt caaccaaatt ttttcaggag gcagatcatg tcattacgtc
      661 tcggcgatac ggctcccgat ttcacgcagg actccagcga aggcccgatc cagttccacg
      721 cttggctggg cgattcctgg ggcgtgctct tttcccaccc cgcggacttc accccggtct
      781 gcaccaccga gctgggcgcc accgccaagc tcaaggacga gttcgccaag cgcggcgtga
      841 aggtgctggc gctctcggtg gacagcgtgg attcgcacaa gaagtggatc ccggacatca
      901 acgagaccca ggggaccacg gtgaacttcc ccatcctggc cgacgccgac aagaaggtgg
      961 ccacgctcta cgacatgctg caccccgcct ccacggccaa cgtgaccgtg cgctcggtct
     1021 tcatcatcga caaccaaaag aagatccggg ccaccatcac ctacccggcc agcacgggcc
     1081 gcaacttcaa cgagatcctg cgggtgatcg actccctgca gttgaccgac aagtacaagg
     1141 tggccacccc ggtcaattgg aaggacgggg acgacgtggt catcgtgccg gccgtgcagg
     1201 accccaagga gatcgcggag cgtttcccga aaggctataa ggccgtcaag ccctacctgc
     1261 gcttgacgcc ccagcccgac aagaaataaa ggccgcgagc ggtccgggcc ccggtggatc
     1321 cccgccgggg cttttttatt ttaggatctt cccccaggcg ttatcatgcc ctatcccttc
     1381 cggaggtctg gcatgaacgt cttccccttg atggcgggag ccctgttggt catggtgatc
     1441 gcctaccgct attacagcgc cttcctggcc gccaaagtgg tggcgctcga cgaccgccgc
     1501 cccacccc
//
LOCUS       DASTJJ010000869         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_364029, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000869 DASTJJ010000000
VERSION     DASTJJ010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364029"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="purE"
                     /locus_tag="VFR02_10505"
     CDS             complement(<1..340)
                     /gene="purE"
                     /locus_tag="VFR02_10505"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9870910.1"
                     /translation="MSEPQALVNILMGSKSDWEVMKNASDTLTQFGVAHESRVLSAHR
                     TPHQTAEYVSGAEARGVKVVIAAAGGAAHLAGVCAAHTLLPVLGVPMESKLDGLDSLL
                     STVQMPAGIPV"
     gene            495..1190
                     /locus_tag="VFR02_10510"
     CDS             495..1190
                     /locus_tag="VFR02_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET9870911.1"
                     /translation="MTSQTFLLIYKAITQGLIIGFCTAALIGPINFMAVRRGIMGGFQ
                     QTFLVGAGAALVDATCAYVVFAGLLKVGFSGLWKIILWGICVVFLLYMAYAFLNEVRD
                     NPEIMGNPKMQRQTQFVDNSFFMGLLLAGSNPFTLIYWIGMVSTLQLSGNIDLTGGAA
                     TSFFSAILVSELVWFSCLGVGVHYSRNLFNRNILKVMTWASGILLVFYCCFMAAKVFL
                     NLVHTGGTPFVNP"
     gene            complement(1319..1498)
                     /locus_tag="VFR02_10515"
     CDS             complement(1319..1498)
                     /locus_tag="VFR02_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HET9870912.1"
                     /translation="MNLEFECPYCGFPNFSPLDPVSGTQEWTTDCENCCRPIVLKARV
                     KHGEVEEFNAEREQD"
     gene            complement(1532..2191)
                     /locus_tag="VFR02_10520"
     CDS             complement(1532..2191)
                     /locus_tag="VFR02_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870913.1"
                     /translation="MRRPIAAFILALTALPAWLAADTIYKDAAVRLLDGTVMVKKADG
                     SPAEALQTGSVVEEGDAVTVYDQSWVVLKTRQGDQIGMLGGTVASFDQLDKEGGDREL
                     RIILQKGILLLRAAGSSTQQSYFEVHTGNVVTSLGDADLIVTYDPAIEHLRLQHLRGR
                     ISVFDKGGEHHLKTEGAIWDWKDGVLQTQGDPDLMDQLDEVNFHRFFNGDPILQPSPT
                     Y"
     gene            2333..>3085
                     /locus_tag="VFR02_10525"
     CDS             2333..>3085
                     /locus_tag="VFR02_10525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9870914.1"
                     /translation="MNRPVMLITGCSSGIGFEAALRFARKGYRVFATLRRPAQAGPLR
                     RAARGLPLEILPLDVDRPASVARALARVRRGAGRLDVLLNNAGFGAFGAFEDFTDQEA
                     LAQYQTNVLGLMRVTRAALPLMRAQGGGRILNLGSLAGRMTFAGIGLYCSSKHAVEAL
                     TEALRLELRPFGIEAAVVEPGSHQTAFKDNRRKNAAFRRGTSPYQHPLEKILEFGDHR
                     SSQAPGAEAVVDAIEKALADRTLRARYPVGRDA"
BASE COUNT          480 a         1003 c         1013 g          589 t
ORIGIN      
        1 ccaccgggat gccggccggc atctgcacgg tggacaggag ggaatccagg ccgtccaatt
       61 tgctttccat gggcacgccc aggacgggca acagggtatg ggccgcgcag acgccggcca
      121 ggtgggccgc gccgccggcg gccgcgatga ccaccttcac gccgcgggcc tcggcgcccg
      181 acacgtactc ggccgtctgg tggggcgtgc ggtgggcgga caggacccgg ctctcatggg
      241 ccacgccgaa ttgggtgagc gtgtcggagg cgttcttcat cacctcccaa tcgctcttgc
      301 tccccatcag gatgttcacc agggcttgcg gttcgctcat ggatcggcct ctccagtcca
      361 agggtattga aaatcacact gagtttcgcc tgggacgggg aaaaatgcaa ctgcgcagga
      421 cggtccaagc gccgaagttc cgccttccgc ccgtcccgcc cctcctgtag aatgtcccca
      481 tggacagtcc cttgatgacc agccaaacct tcctgctcat ctacaaggcc atcacccaag
      541 gcctcatcat cggcttttgc acggcggccc tgatcgggcc gatcaacttc atggccgtgc
      601 gccggggcat catggggggg ttccaacaga ccttcctggt gggggcgggc gcggccctgg
      661 tggacgccac ctgcgcctac gtggtcttcg cggggctctt gaaggtgggg ttttcaggcc
      721 tttggaagat catcttatgg ggcatctgcg tggtcttcct cttatatatg gcctacgcct
      781 tcctcaatga ggtgcgggac aaccccgaga tcatggggaa cccgaagatg cagcgccaga
      841 cccagttcgt ggacaattcc ttcttcatgg gcctgctttt ggcgggcagc aaccccttca
      901 ccctcattta ttggatcggc atggtcagca ccctgcagct gtcgggcaac atcgacctga
      961 cggggggggc ggccacctcc ttcttctcgg ccatcctggt cagcgagctg gtctggttct
     1021 cctgcctggg ggtgggggtg cactacagcc gcaacctctt caaccgcaac atcctcaagg
     1081 tgatgacctg ggcctcgggc atcctgttgg tcttttattg ctgcttcatg gcggccaagg
     1141 tgttcctgaa cctggtgcac accgggggaa cgcccttcgt caacccatag gcctaaaggc
     1201 ggttttgagt tctaagtttt tagttcttag ttgagtcgtt caattaagaa ctcagcactc
     1261 aaaactgaag gtagggcggc cctgttcccg gctgccgggg caagcctgcc cctccgggtc
     1321 aatcttgctc ccgctccgcg ttgaattcct cgacctcccc atgtttgacc cgcgccttca
     1381 ggacgatcgg ccggcagcaa ttctcgcagt cggtggtcca ttcctgcgtg ccggacaccg
     1441 ggtccagggg ggaaaagttg gggaagccgc agtaggggca ctcaaattcc aggttcatgg
     1501 acgctcctgg gtcgggtcgg ggggaccttg atcaataagt gggcgagggc tgcaggatag
     1561 ggtcgccgtt gaagaaccgg tggaagttca cctcgtccaa ctggtccatc aggtccgggt
     1621 cgccctgggt ctgcaggacg ccgtccttcc agtcccagat ggccccctcg gtcttcaggt
     1681 ggtgctcgcc gcccttgtca aaaacgctga tgcgcccccg caggtgctgg agcctcaggt
     1741 gctcgatggc ggggtcgtag gtcacgatca ggtccgcgtc gcccagcgag gtcaccacgt
     1801 tgccggtatg cacctcgaag tagctctgtt gggtcgagga accggccgcc cgcagcagca
     1861 ggatgccctt ctgcaggatg atgcgcagct cccggtcgcc gccttcctta tccaactggt
     1921 cgaaggaggc caccgtgccg cccagcatgc cgatctggtc gccctgccgg gtcttgagga
     1981 cgacccagct ctggtcgtac accgtgaccg cgtcgccttc ctcgaccacg ctgccggtct
     2041 gcagggcctc ggcgggggaa ccatcggcct tcttcaccat gacggtcccg tccagcaggc
     2101 ggacggccgc gtccttgtag atggtgtccg ccgccagcca ggcgggcagg gccgtcaggg
     2161 ccaggatgaa ggcggcgatc ggtcggcgca tggggtctcc tcacggggtt gggatggcgg
     2221 acggctttct atcctaaggc atggcggggc ccttggggag gactttttcc gcccgggtcc
     2281 gggcccgcct gtgatgaaat ggggcgtgcg aaaaccccgg agggaggcgg ccttgaaccg
     2341 acctgtcatg ctcatcaccg gttgttccag cggcatcggc tttgaggccg ccctgcgctt
     2401 cgcccgcaag ggctaccggg tcttcgccac cctgcggcgc cccgcccagg cgggcccctt
     2461 gcggcgggcg gcccggggct tgcccctgga gatcctgccg ctcgacgtgg accggcccgc
     2521 ttcggtggcc cgggccctgg cgcgtgtgcg gcgcggggcc gggcggttgg acgtgctcct
     2581 caacaacgcg ggtttcggcg ccttcggggc tttcgaggat ttcaccgacc aagaggcgtt
     2641 ggcccagtac cagaccaacg tcttggggtt gatgcgggtg acccgggccg ccctgcccct
     2701 gatgcgcgcc cagggagggg ggcgcatcct gaacctcggg tccctggcgg gccgcatgac
     2761 cttcgcgggc atcgggctct attgttccag caagcacgcc gtggaggccc tgaccgaggc
     2821 cctgcggctg gaactgcggc ccttcggcat cgaggcggcc gtggtggagc ccggatcgca
     2881 ccaaacggcc ttcaaggaca accggcgcaa gaacgccgct ttccgccggg ggacatcccc
     2941 ctaccagcat ccattggaaa aaatactgga gttcggcgac catcgatcct cccaggcccc
     3001 gggggcggaa gcggtggtgg acgcgatcga aaaggccctg gcggaccgga cgctccgggc
     3061 ccgctatccg gtgggccggg atgcc
//
LOCUS       DASTJJ010000870         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1587348, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000870 DASTJJ010000000
VERSION     DASTJJ010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587348"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFR02_10530"
     CDS             complement(<1..519)
                     /locus_tag="VFR02_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9870915.1"
                     /translation="MKTAFFNTKSYDRKFFDEANREGRQEIRYLEAHFNASTAPLAKG
                     FEAVCVFVNDQVDAASLAVLRGEGVRYVLTRSAGFNHIDLPAAKAAGIRVFRVPDYSP
                     HAVAEHALGLILSLDRKIHRSYHRVREGNFSLEGLLGFDLQGKTVGVVGLGKIGQAAA
                     RILKGFGCEVLAS"
     gene            627..1553
                     /locus_tag="VFR02_10535"
     CDS             627..1553
                     /locus_tag="VFR02_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET9870916.1"
                     /translation="MIHTLTLNPSIDQHVTVAKLVKDDTLRALSVHQDPGGKGINVSR
                     GLHCLGQDTRAFTFTGGHAGRIFEHLLAREKVTCTRVRVGGETRINQILTDLSDRTQT
                     RISMPGPPVDARALKRMARKVASAQAPYWVLGGSLPEGVPADTYRTFIQSLQAKGERC
                     VLDADGEALRGGVEARPFLIKPNEYELEHLVGRPLSDEADILKAAQGLCAQGVGLVAV
                     TLGGRGALVAGPREAYSLKAPPVEARSKVGAGDSFIAGFLNDWVQKGDLLSAACSGVA
                     AGTAAVVKDGTKLFSREDHARFLPLVQWRRLA"
     gene            1632..>1834
                     /locus_tag="VFR02_10540"
     CDS             1632..>1834
                     /locus_tag="VFR02_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870917.1"
                     /translation="MGKRQTYALAGSLVGTGAPFGALVLQYLTTRPWGAPLPFVDAQW
                     SAHPFFYWYMLLSTCLVLSGLGY"
BASE COUNT          304 a          659 c          573 g          298 t
ORIGIN      
        1 ggaggccaac acctcgcacc cgaacccctt caggatccgg gcggccgcct ggccgatctt
       61 acccaagccc accaccccca cggtcttgcc ttggaggtcg aaccccaaca ggccctccag
      121 ggaaaagttc ccttcccgca cccggtggta ggaccggtgg atcttccggt cgagggacag
      181 gatcagcccg agggcgtgct ccgccacggc gtggggcgaa taatcgggca cccggaagac
      241 ccggatgccc gccgccttgg ccgccggcag gtcgatatgg ttgaagcccg cgctgcgggt
      301 gagcacatac cgcacgccct ccccccgcaa caccgccagg gaggccgcgt ccacctggtc
      361 gttgacgaag acgcagaccg cctcaaagcc cttcgccagg ggggccgtgg aggcgttgaa
      421 atgcgcctcc aggtaccgga tctcctgccg cccttcccgg ttggcctcgt cgaagaattt
      481 ccggtcgtaa cttttggtgt tgaaaaaggc cgttttcatg gcgccctccc ttttccccaa
      541 gataggcccc cggatcttcc cctaaaatga tccaggtcat aaagcccggg gcgccttggt
      601 ggcatcctgg agctggagaa agcgccatga tccataccct gaccctgaac ccttccatcg
      661 accagcacgt caccgtggcc aaactggtca aggacgacac cctgcgggcc ctgtccgtgc
      721 accaggaccc gggcggcaag ggcatcaacg tgtcccgggg cctgcactgc ctgggacagg
      781 acacccgggc cttcaccttc acgggcggcc acgccgggcg catcttcgaa cacctgctgg
      841 cccgggaaaa ggtcacctgc acccgggtcc gggtaggggg cgagacccgc atcaaccaga
      901 tcctgaccga cctgtccgac cggacccaga cccgcatcag catgccgggc ccgcccgtgg
      961 atgcccgggc cctgaagcgc atggcccgaa aggtcgcctc ggcccaggcc ccctattggg
     1021 tcctgggcgg aagcctcccc gaaggcgtcc ccgcggacac ctaccggacc ttcatccagt
     1081 ccctccaggc caagggggaa cgttgcgtgc tggacgccga cggggaagcc ctgcgggggg
     1141 gtgtggaggc ccgccccttc ctcatcaaac ccaacgaata tgagttggag catctggtgg
     1201 ggcgtcccct gtcggacgag gccgacatcc tcaaggccgc ccaaggcctc tgcgcccaag
     1261 gggtggggct ggtggcggtc accctgggcg gccggggggc gctggtggcc ggaccccggg
     1321 aggcctattc gcttaaggcg ccgcccgtgg aggccaggag caaggtgggg gccggggatt
     1381 ccttcatcgc cggattcctg aacgactggg tccaaaaggg cgacctcttg tccgccgcct
     1441 gttcgggcgt ggccgccggc accgccgccg tggtcaagga cggcaccaag cttttcagcc
     1501 gggaggacca tgcgcgcttt ctccccctcg tccaatggcg gcgcctcgcc tgagcttttg
     1561 tccccctcta tgtcgccggt cataaagccc gacgcgccct cctggcagac tgggtccatc
     1621 aggagggcac catgggaaaa agacagacct acgccttggc cggatcttta gtgggcacgg
     1681 gggcgccctt cggcgccttg gtgctccaat acctcaccac ccgcccctgg ggcgcgccgc
     1741 tccccttcgt ggacgcccaa tggtccgccc accccttctt ttactggtac atgctcctga
     1801 gcacctgcct ggtgctgtcg ggcctcggct atta
//
LOCUS       DASTJJ010000871         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1342189, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000871 DASTJJ010000000
VERSION     DASTJJ010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342189"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..438)
                     /locus_tag="VFR02_10545"
     CDS             complement(67..438)
                     /locus_tag="VFR02_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870918.1"
                     /translation="MSFRPLAILLGAFLLALEPALPPAPMGHIQPQDRGLSQPVKGLC
                     GLPACRPHKPSLCGRPPAPGLYASGCVPVNKGFYAPPATAKWASRDRVFFPVRSARLN
                     PAVRPLPHPFQAEPIPPPPRA"
     gene            567..>1331
                     /locus_tag="VFR02_10550"
     CDS             567..>1331
                     /locus_tag="VFR02_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9870919.1"
                     /translation="MKRFLLILLLPALLAYLTIVAAFALKQKDLVFVPRPLDHVTTPL
                     TYGQAYRVVDFPAADGVTLNGWWMRHPGGRHPALVYCHGNAANLSLLAQVASLFYGYG
                     FDALIFDYRSYGDSGKAPLSEAAVDADALGAYRWVESQGYPESRVFIWGHSLGSSVAA
                     ELATKTHPAGLFLEGAFPSIYAVSRARFPWLLVFPFMIEDKFRTEDYVKERSCPLLEV
                     HGEKDTVIPIRLGREVFADAVPPKQWIEVPGMDHLDF"
BASE COUNT          225 a          406 c          465 g          235 t
ORIGIN      
        1 catgggattc tccttcattc aagatggcgg acgggtcgcg tccgcggaac aacgcttgaa
       61 aaagggtcag gcgcggggtg gcggcgggat gggttcggct tggaagggat gcggcaaagg
      121 acggacggcg gggttgagcc gggcggaccg gaccgggaag aacacccggt cgcgggaagc
      181 ccacttggcg gtggcgggcg gcgcgtagaa acccttgttc accggcacgc agccggaggc
      241 gtagagcccg ggggccgggg ggcggccgca aagcgacggc ttgtggggac ggcaggcggg
      301 caggccgcag aggcccttca cgggttggga caagccccgg tcctggggct ggatatgccc
      361 catcggcgcc ggcggcaggg ccggttccag ggcgagcagg aaagccccca ataggatggc
      421 aagcggtcga aaactcatga cggacaagtt attccctccg gcccggcgat tgcaatagga
      481 ataggggccg ggaccggggc ctgttacaag atcggcgccg gaaggcccct catgctaaac
      541 tcggcgcatt ccatcgcgag gtgcccatga aacgcttcct gctcatcctg ttgctcccag
      601 ccctcttggc ctacctgacc atcgtggcgg ccttcgccct caaacaaaag gacttggtct
      661 tcgtgccccg gcccctggac catgtgacca cgcccttgac ctacggccag gcctaccggg
      721 tggtggactt ccccgccgcc gacggcgtga ccttgaacgg ctggtggatg cgccacccgg
      781 gcggcaggca cccggccctg gtctattgcc acggcaacgc ggccaacctg tcgcttttgg
      841 cccaggtggc ctccttgttc tatggatatg ggttcgacgc gttgatcttc gattaccgaa
      901 gctacgggga cagcgggaaa gcccccctga gcgaggcggc ggtggacgcg gacgcgttgg
      961 gcgcctaccg gtgggtggag tcccagggtt atcctgaatc ccgcgttttc atctggggcc
     1021 attcgctcgg gtcgtcggtg gcggcggagt tggccacgaa gacccatccc gcagggctct
     1081 tcttggaagg cgccttccct tcgatctacg cggtctcccg ggcccgcttc ccctggcttt
     1141 tggtcttccc cttcatgatc gaggacaagt tcaggacgga ggattacgtt aaggaaaggt
     1201 cctgcccgct gcttgaggtc catggggaaa aggacaccgt gatcccgatc cggttgggcc
     1261 gggaggtctt cgcggacgcg gtgcctccca aacaatggat cgaggtgccc ggcatggacc
     1321 atttggactt c
//
LOCUS       DASTJJ010000872         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_486955, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000872 DASTJJ010000000
VERSION     DASTJJ010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486955"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="VFR02_10555"
     CDS             complement(<1..1752)
                     /locus_tag="VFR02_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870920.1"
                     /translation="MSYDNNFLNAPVFFNPPGTPTITPTPKQAVSGPLVDAGPLRSVA
                     YVSSSQNNYPPPGAIPTGIVPTLVATGFSDSDGIDYFPVLNRLLISDHGSFNGVGHNF
                     FTVGANGSIAPFTAINDLPDEINITIARDECNGNSLGGFVAGTIFTEGTGSGTVGKIS
                     PDGASFQNPWVTLPGESGRKGLWLDRTGIFGGDLLVINNTGGFWRVNAQGGYSKVATL
                     PSGDYDGLTTVPNDPARFGPWAGTALTAQEQNSTIYSIAPDGAVSSYFFAPGVGAAIN
                     DMLLVPPNENLFMTAYNSFVYGVPASQLSGIAGDFLVPGEISGQVWDLRWDGSQYVST
                     VLFNVPGAGAEELAFGPAGAGPVSTSLSPVTMTAAASDQNAPPRPLTYQWTQVSGAGH
                     ALFNDPTLLDPVVTFDHSGNYVLQLCANNGAYTSCASTTVVVSTMACNQPPPLPTNTV
                     TLTPTVTATPTITWTATPYPTGFLTLTFTPSPSPTPTSTPTLSPTPSRSPTPTPTATW
                     TATLVPGAFVARIAVGADAPVTDHLGQVWEADQDYFPGEGAQGQVGEAQAYYTTGGIN
                     GTQDPALYQHFRQDIGWE"
BASE COUNT          302 a          529 c          644 g          299 t
ORIGIN      
        1 ttcccagccg atatcctgcc ggaaatgttg gtagagggcc gggtcctggg tgccgttgat
       61 gccgccggtc gtgtagtagg cttgggcctc gcccacctgc ccctgggcgc cttccccggg
      121 gaaataatcc tggtcggctt cccagacctg gcccaagtgg tccgtcacgg gggcgtccgc
      181 ccccacggcg atacgggcca cgaaagcgcc cggcaccaag gtggccgtcc aggtggccgt
      241 cggcgtgggc gtggggctgc gggacggcgt cggggaaagg gtcggcgtgg aggtcggggt
      301 ggggctgggg ctcggggtga aggtcagcgt gaggaacccg gtcggatagg gcgtggccgt
      361 ccaggtgatg gtgggcgtgg ccgtcaccgt cggggtgagg gtgaccgtgt tggtcggcag
      421 ggggggcggc tggttgcagg ccatggtcga gaccaccacc gtggtgctcg cgcaggaggt
      481 ataagccccg ttgttggcgc acaattggag cacgtaattc cccgaatggt cgaacgtcac
      541 caccgggtcc aagagggtgg ggtcgttgaa aagggcgtgg cccgcgccgg aaacctgggt
      601 ccattggtag gtcaggggcc gggggggcgc gttctggtcc gaggcggcgg cggtcatggt
      661 gaccgggctg agggaggtgg aaaccggccc ggcccccgcg gggccgaaag ccagttcctc
      721 ggcccccgcc cccggaacgt tgaacaaaac cgtggagacg tattggctgc cgtcccagcg
      781 caggtcccaa acctggcccg atatttcccc cggcaccagg aaatcccccg cgatgccgga
      841 caattgggaa gccggcacgc cgtacacaaa gctgttgtag gccgtcatga aaagattctc
      901 gttgggcggg accaggagca tgtcgttgat ggcggccccc acgcccgggg cgaaaaagta
      961 ggaagagacc gccccatcgg gcgcgatgga atagatggtg ctgttctgct cttgggcggt
     1021 caaggccgtg ccggcccagg ggccaaaacg cgccgggtcg ttcgggacgg tggtcaagcc
     1081 gtcataatcc cccgagggga gcgtcgccac cttggaataa cccccctggg cgttcacccg
     1141 ccagaaaccg ccggtgttgt tgatgaccaa caggtcgccg ccgaagatcc ccgtccggtc
     1201 cagccaaagc cccttgcgcc ccgattcacc cggcagggtg acccatgggt tctggaagct
     1261 tgcgccgtcc gggcttattt ttcccacggt ccccgaaccc gtcccctcgg tgaagatggt
     1321 gccggccacg aagcccccca ggctgttccc gttgcactca tcccgcgcga tcgtgatgtt
     1381 gatctcatcc ggcaggtcgt tgatggcggt gaaaggcgcg atcgatccat tggcgcccac
     1441 cgtgaagaaa ttgtgcccca ccccgttgaa gctgccgtgg tcgctgatga gcagccggtt
     1501 caagaccggg aaataatcga tcccatccga gtcggagaac ccggtcgcca ccaacgtcgg
     1561 aacgatgccg gtcgggatgg cgcccggcgg cgggtaattg ttttgcgagg aactgacgta
     1621 ggcaacggac cgcagaggtc cggcatccac gaggggcccc gaaacggctt gcttgggcgt
     1681 gggggtgatg gtgggcgtgc ccggcgggtt gaagaagacc ggggcgttga ggaaattgtt
     1741 gtcatagctc acttccatga gggggtgcgc caag
//
LOCUS       DASTJJ010000873         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1587832, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000873 DASTJJ010000000
VERSION     DASTJJ010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587832"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..>1851
                     /locus_tag="VFR02_10560"
     CDS             85..>1851
                     /locus_tag="VFR02_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9870921.1"
                     /translation="MRKSKEPILKGPEMQAAFHEEDAQVRLSNIKIGCYFIIFGMPGG
                     TSLDQQVYPQMVPYFLKVRLLCGLLGLVALGLLYTPLGKRYLKVFGIALPLLPVGCIS
                     WMIYCTEGAASPYYAGISMTILAVGLVWSWTFLENLLTGVLSIGIYYAACRLHGAVDP
                     AHGIAYNNFYFLVLVDLLVIIMGYFNEQLRLREFALRFELDHNKRMLEESNRKLVELD
                     QMKSRFFANISHELRTPLTLLVGPLETLQQGKAAGMDEETRSTLRMMYANAMRLLKLI
                     NDLLDLVKLESGRLEVAREPLEVEGFLKGLAQSISVAARDKRIKLVVSVPPLPTALLD
                     PDKLEKIVLNLAFNAIKFTPSGGRVELSAAREGDRLALRVKDTGMGIAQDNLPYIFDR
                     FWQADSSANRKYQGTGIGLALVKELAEAQGGTVSVESEVGKGTEMTVRLPFEEAPLPA
                     AVPAFPQAAEAPAARPDEWLSKLYRRAELFPTLTALGDTLKADSYAPGRKPRILVADD
                     EPDMLSFLKYQLQKQYEVLEAVDGRQALEKARQFQPDLALLDMMMPEMDGVEVCRQIK
                     AQVSTQAIPVIMLTARADDETKM"
BASE COUNT          329 a          610 c          598 g          314 t
ORIGIN      
        1 cgggtgttga cggacgccac cggggtgaac atctttttgg aggtccgcaa gccgggttga
       61 ccggcgggcg cgggaaagcg ggatatgcga aagtccaagg aaccgatcct gaagggcccg
      121 gaaatgcagg ccgccttcca cgaggaggac gcccaggtcc gcctgtccaa catcaagatc
      181 ggctgttatt tcatcatctt cggcatgccg gggggcacct cgctggacca gcaggtctat
      241 ccccagatgg tgccctattt cctcaaggtg cgcctcctct gcggcctctt gggcctggtg
      301 gccctggggc tgctctacac ccccttgggc aaacgctacc tgaaggtctt cggcatcgcg
      361 ctgcccctgc tccccgtggg ctgcatttcc tggatgatct actgcaccga gggggcggcc
      421 tcgccctatt acgccggcat cagcatgacc atcctggccg tggggctggt ctggtcctgg
      481 accttcctgg agaacctcct gacgggcgtc ttgagcatcg ggatctacta cgcggcctgc
      541 cgcctgcatg gcgcggtgga ccccgcccac ggcatcgcct acaacaattt ctatttcctg
      601 gtcctggtgg acctcctggt catcatcatg ggctatttca acgagcagtt gcgcctgcgg
      661 gaattcgccc tgcgcttcga gctggaccac aacaagcgca tgctggagga atccaaccgc
      721 aagctggtgg agctggacca gatgaagagc cggttcttcg ccaacatcag ccacgagttg
      781 cgcacgcccc tcaccctctt ggtcggcccc ctggagaccc tgcagcaggg caaggccgcc
      841 ggcatggacg aggagacccg gtccaccctg cgcatgatgt acgccaacgc catgcgcctg
      901 ttgaagctca tcaacgacct gttggacctg gtgaagctgg agtcgggccg cctggaagtg
      961 gcccgggagc cgctggaagt ggagggcttc ctgaagggcc tggcccagtc catctcggtg
     1021 gcggcccggg ataaacgcat caagctggtc gtttccgtgc cgcccttgcc caccgcgctc
     1081 ctggacccgg acaagctgga aaagatcgtg ctcaacctgg ccttcaacgc catcaagttc
     1141 acgccgtcgg ggggccgggt ggaactgtcg gccgcccggg agggggaccg cctggcgctg
     1201 cgcgtcaagg acacgggcat gggcatcgcc caggacaacc tgccctatat cttcgaccgg
     1261 ttctggcagg ccgattcctc ggccaaccgc aagtaccagg gcacgggcat cgggttggcc
     1321 ctggtgaagg aactggccga ggcgcagggg ggcacggtct cggtcgagag cgaggtgggc
     1381 aaggggaccg aaatgaccgt ccgcctgccc tttgaggagg cccccctgcc cgccgccgtg
     1441 cccgccttcc cccaggcggc ggaggcgccg gcggcacggc ccgacgaatg gttgtccaag
     1501 ctctaccggc gcgcggaact tttccccacc ctgacggccc tgggggacac gctgaaggcc
     1561 gactcctacg ccccgggccg gaagccgcgg atcctggtgg ccgacgacga acccgacatg
     1621 ctcagcttcc tcaaatacca gcttcaaaaa caatatgaag tgctggaggc cgtggacggc
     1681 cgccaagcgc tggaaaaggc ccggcaattc cagcccgacc tggcgctgct ggacatgatg
     1741 atgcccgaga tggacggcgt ggaagtgtgc cggcagatca aggcgcaggt gtccacgcag
     1801 gcgatcccgg tgatcatgct cacggcccgc gccgacgacg agaccaagat g
//
LOCUS       DASTJJ010000874         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1709020, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000874 DASTJJ010000000
VERSION     DASTJJ010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709020"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..764)
                     /locus_tag="VFR02_10565"
     CDS             complement(183..764)
                     /locus_tag="VFR02_10565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870922.1"
                     /translation="MNAFEQRYQEGVPPWEIGRPQAWVVELFGQDAFGGEVLDVGCGT
                     GENSLFLAAQGLSVTGVDFSRTAIELAQEKAVQRGLAPRFRVGNALRLQLLNRAFDTA
                     LDSAVFHVFTDKERAFYAESLAGALAPGGAAYVVCFSEREPEGWGPRRVTQGEIQRTF
                     QKRFDVALIQRAYYETNMRPEPVPSWLACLRKK"
     gene            complement(767..1699)
                     /gene="mdh"
                     /locus_tag="VFR02_10570"
     CDS             complement(767..1699)
                     /gene="mdh"
                     /locus_tag="VFR02_10570"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9870923.1"
                     /translation="MSQRPKITVVGAGNVGASCAVWMAERDLGDIVLLDIPAAKDMPK
                     GKALDMQQVGPISNYNSHLTGTTDYADTKDSDICVITAGMPRKPGMSREDLVAINQGI
                     VQDVVKKLVALSPDTLLIIVTNPLDTMAYVAYKAAGLPKHRVIGQAGVLDSARMRTFL
                     AAELGVAVENVNASVIGGHGDEMVPLVGYSTVAGVPIANYLSADKIAAIVERTRKGGG
                     ELVNLLGVSAWYAPGAAAGFMVEAILKDKHLIVPCAAYLEGEYGLKDMYFGVPVKLGR
                     KGLEQVLEYKLTEDEKGMIQKSAALIRETMKALK"
     gene            1837..2370
                     /gene="cysC"
                     /locus_tag="VFR02_10575"
                     /pseudo
     CDS             1837..2370
                     /gene="cysC"
                     /locus_tag="VFR02_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
     gene            2372..>3233
                     /locus_tag="VFR02_10580"
     CDS             2372..>3233
                     /locus_tag="VFR02_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870924.1"
                     /translation="MRPAPAVRAALFLAAATAAAFGGFLNPWGSRVVSLAGEDLTGQF
                     VWWRQFGFDQLRQGHLALWNPHLFSGAPFFGGFQSALLYPPNWLYLFLPLGFAINFTV
                     ALHVFLAGYFTYLWTRFQGFRPEACLLAALMFMFGGSYFLHIVPGHLPNLCTMAWIPL
                     VFLALDGWARDRRAGWLLLGAGAFAAQVLAGHIQYFYYTALAAGLYTLARLPREPRRW
                     SFLAGPAAMLGAAVLLTAVQLTAGWDATQASVRGQALPLDLILSNDLMAERVWSWWVP
                     FFYGSAVHRDY"
BASE COUNT          498 a         1089 c         1064 g          582 t
ORIGIN      
        1 ggttgacgct ccccaatttc cagaccaccc tgaagttcct ggccgaggtg gtgcgggccg
       61 aggccctcac cgagatgggc cgcgcgctcc agcgctcggg gctcaagacc ctggccatca
      121 acaaggaaga cgtgacccgg atggaaaaat acctgatcga taagatgagg aaaaagtagg
      181 ggttacttct tgcgcaggca ggccagccag gaagggacgg gctcgggccg catgttggtc
      241 tcgtaatagg cccgctggat cagggccacg tcgaagcgtt tttggaaggt ccgctggatc
      301 tccccctggg tcacccgccg cggcccccac ccctccggtt cccgctcgct gaaacagacc
      361 acataggccg ccccgccggg ggccagggcc cccgccaggc tttcggcgta gaaggcgcgt
      421 tccttgtcgg tgaagacgtg gaagaccgcc gaatccagcg cggtgtcgaa ggcgcggttc
      481 aagagctgca gccgcagggc gttgcccacc cggaagcggg gcgccaggcc ccgttggacg
      541 gccttttcct gggccagttc gatggccgtg cgggaaaagt ccaccccggt caccgagagc
      601 ccctgggcgg ccaggaagag cgaattctcc cccgtgccgc aacccacgtc caggacttcg
      661 cccccgaagg cgtcctggcc gaacaactcg accacccagg cctgcggccg gccgatctcc
      721 cagggcggca cgccctcctg gtagcgttgc tcgaaggcgt tcaaggttat ttgagggcct
      781 tcatggtctc gcggatgagg gccgcggact tttggatcat gcccttctcg tcctcggtga
      841 gcttgtactc caagacctgt tccaggccct ttcggcccag cttcaccggc acgccgaaat
      901 acatgtcctt caggccgtac tcgccctcca aataggccgc gcagggcacg atcaggtgct
      961 tgtccttcaa gatggcctcc accatgaaac cggcggccgc gcccggggcg taccaggccg
     1021 acacgcccaa gaggttcacc agctcgccgc cgcccttgcg ggtgcgttcc acgatggccg
     1081 cgatcttgtc ggccgacagg tagttggcga tgggcacgcc cgccacggtg gaataaccga
     1141 ccagggggac catctcgtcg ccgtggccgc cgatgaccga ggcgttcacg ttctcgaccg
     1201 ccacgcccag ctccgccgcc aggaaggtgc gcatgcgggc cgaatccagc acgccggcct
     1261 ggccgatgac ccggtgtttg ggcagccccg cggccttgta ggccacgtag gccatggtgt
     1321 ccagcgggtt ggtcacgatg atgaggaggg tgtcggggga cagcgccacc agttttttca
     1381 ccacgtcctg cacgatgccc tggttgatgg ccaccaggtc ctcccggctc atgccgggtt
     1441 tgcggggcat gcccgcggtg atcacgcaga tgtccgagtc cttggtgtcc gcgtagtccg
     1501 tggtgccggt caaatgggag ttatagttgg agatggggcc cacctgctgc atgtccaggg
     1561 ccttgccctt gggcatgtcc ttggccgccg ggatgtcgag cagcacgatg tcgcccaggt
     1621 cccgttcggc catccagacc gcgcaggacg cgccgacgtt ccccgctccg accacggtga
     1681 tcttgggacg ctgggacatg gaaagcctcc aaaaaaggga tgggaaaacc gggggacagt
     1741 ttatccaagc cccgggggat tgtccatgcg gccggggccg cccccgcttg acagaacccg
     1801 ggggggtcat tagcttgggg gcacgcgcga aagggggaac acttggccaa gggattcacg
     1861 ctttggttca ccgggctgtc cggctcgggc aaatccacca tttcggaacg ggtggcccag
     1921 cggttgaagc gggatgggct ggccgtcgag gtgctggacg gcgacatcgt gcgcacgaac
     1981 ctttccaagg gcctgggttt ttcccgggag gaccgcgacg agaacatcaa gcgcgtgggg
     2041 ttcgtgtgcc agctcctgac gcgcaacggc gtggccgcgg tggcctcggt catttcgccc
     2101 taccgggcgg cccgggacca caaccgcaag aagatcgggg atttcatcga ggtctacgta
     2161 cgctgcccgg tggaggtctg cgcggggcgc gacgtgaagg ggctctacca aaaggcccag
     2221 gccggcgaga tcaagggttt cacgggcgtg gatgacccct atgaggagcc gctcaacccc
     2281 gaggtcatct gcgacaccga ccaggaaagc gtggacgaaa gcgtggccaa ggtgctggcc
     2341 cgggtgaagg ccctgggcta cctttcctga ggtgagaccg gcccccgccg tccgcgcggc
     2401 gcttttcctg gccgccgcca cggcggcggc cttcggcggt ttcctcaacc cctggggttc
     2461 ccgcgtggtc tccctggcgg gcgaggacct gaccggccag ttcgtttggt ggcgccagtt
     2521 cgggttcgac caactgcgcc agggccacct ggccctctgg aacccccatt tattctcagg
     2581 cgcgcccttc ttcggcggct tccagtccgc cctcctgtac ccccccaact ggctttacct
     2641 tttcctgccg ctgggtttcg ccatcaattt caccgtggcc ctgcacgtct tcctggcggg
     2701 ctatttcacc tacctttgga cccgcttcca gggcttccgc cccgaggcct gcctgctggc
     2761 cgcgctgatg ttcatgttcg gcggctccta ctttcttcat atcgtcccgg gccacctgcc
     2821 caacctctgc accatggcct ggatcccgct ggtgttcctg gcgctggacg gctgggcccg
     2881 ggaccgccgg gccggttggc tgctcctggg ggccggggct ttcgcggccc aggtgctggc
     2941 cggccatatc cagtactttt actacacggc cctggcggcg gggctttaca ccctggcccg
     3001 cctgccccgg gaaccgcgcc gatggtcctt cctggcgggc ccggcggcca tgctgggggc
     3061 ggcggtcctg ctcacggccg tccaactgac cgccggatgg gacgccaccc aggcgagcgt
     3121 gcggggccaa gccctgccgc tggacctgat cctttccaac gacctgatgg cggagcgggt
     3181 ctggtcctgg tgggtaccct tcttttacgg cagcgccgtc caccgggact att
//
LOCUS       DASTJJ010000875         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1220088, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000875 DASTJJ010000000
VERSION     DASTJJ010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220088"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1365
                     /locus_tag="VFR02_10585"
     CDS             154..1365
                     /locus_tag="VFR02_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9870925.1"
                     /translation="MRVALVNNYLFPKGGAERVLLDEGSWLERRGEQVSYFGLQDPAN
                     PPGLGGEWLPAGGELRARGFRAGITGLMRFIDNREAGRAFGRFLAGVKPELVHVHNLY
                     GGLTTAVLGPAREAGVPVVMTLHDYKLVSPSPNLVSRGRPCVDCQVRPGTPLCLLRDC
                     LGSPGKTLAHWLESEYNRRRRAYGSVSAFIAPSRFMAEKAAAAGWDPARIHWVPNAME
                     PQATDATPVEGGYFFFAGRMAEEKGVGFLLEAWPGVRGELRLAGDGGSLDRYRQLAPP
                     GVEFLGRVAPEGLGDLYRGALAVVVPSLWYENASMTVLEAMAHGRPVVATRMGGLPEQ
                     VEDGVTGILVEPGDRAGLRRALTRLAEEPGLAARLGRAGRARLEERFGHPRHGEALLA
                     VYASVLGRGTR"
     gene            1362..>1724
                     /locus_tag="VFR02_10590"
     CDS             1362..>1724
                     /locus_tag="VFR02_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870926.1"
                     /translation="MKAPRGKILMACCNHWTSPFQVGSHQLAREFVRLGYEVAFVSDP
                     VSPFHLAAFGDLTRERFRIHRSGGMRDLGGGLWAYVPLTLVPPQRGPLLGSDWAYHSW
                     YRFTLPSVVEKARRAGFGK"
BASE COUNT          216 a          538 c          667 g          303 t
ORIGIN      
        1 ggtgggtttt tgcatcagcc gcgatccccg gctctattgg ctggcggcgg cccctcccct
       61 ttacgcccta ttgctgtcgg tcttgggggc cttgggtccg gaagattgga agagcctgcg
      121 gtccgtcctg gcccggcgca agggaagccg gacttgaggg tcgccctggt gaacaattac
      181 ctgttcccca aagggggggc ggaacgggtg ctgttggacg agggatcctg gttggagcgc
      241 cggggcgaac aggtgtccta cttcggcctc caggatcccg ccaatccgcc gggcctcggg
      301 ggggagtggc tcccggccgg cggggaactg cgggcccggg gcttccgggc cgggatcacc
      361 gggctgatgc gtttcatcga caaccgggag gccggacggg ccttcggccg tttcctggcg
      421 ggggtcaagc ccgagctggt ccatgtccac aacttgtacg gcgggctgac caccgcggtc
      481 ctgggcccgg cccgggaggc gggggtgccc gtggtgatga ccctccacga ttacaagctg
      541 gtgagcccct cgcccaacct ggtctcccgt ggccgcccct gcgtggattg ccaagtgagg
      601 cccggcacac cgctttgcct tctgcgggac tgcctgggat ccccggggaa gaccctggcc
      661 cattggttgg aatccgaata caaccgccgg cggcgcgcct atgggtcggt gtcggccttc
      721 atcgcgccca gccgcttcat ggccgagaag gccgcggcgg cggggtggga cccggcgcgg
      781 atccattggg tccccaacgc gatggagccc caggccaccg acgcgacccc ggtggagggg
      841 ggctatttct ttttcgcggg ccgcatggcc gaggagaagg gggtcggttt tttattggag
      901 gcttggcccg gggtgcgggg ggaattgcgg ttggccgggg acggcggatc gttggaccgc
      961 taccggcagc tggccccgcc gggggtggaa ttcctggggc gggtggcgcc ggaaggcctg
     1021 ggggaccttt accggggcgc cctcgcggtg gtggtccctt ccttatggta tgagaacgcc
     1081 tccatgacgg tgttggaagc catggcccac ggccggcccg tggtggccac ccgcatgggc
     1141 gggcttcccg agcaggtgga ggacggcgtg accgggatcc tggtggaacc cggggaccgg
     1201 gcgggactgc ggcgggccct gacgcggttg gccgaagagc cggggctggc ggcgcgcctg
     1261 ggccgggcgg gccgcgcccg attggaggag cgtttcggcc acccgaggca tggggaagcc
     1321 ctgttggcgg tctacgcctc cgttctggga agggggacgc gatgaaggcg ccgaggggca
     1381 agatccttat ggcctgctgc aaccactgga cctcgccctt ccaggtgggg agccaccaac
     1441 tggcgcggga attcgtgcgg ttgggctatg aggtggcctt cgtttcggac ccggtctccc
     1501 ccttccactt ggcggccttc ggtgacctga cccgggagcg gttccggatc caccggtcgg
     1561 ggggcatgcg cgatctcggg ggcgggcttt gggcctatgt tcccctcacc ctggtcccgc
     1621 cccaacgggg gcccctgctg gggtcggatt gggcctacca ttcctggtac cgtttcaccc
     1681 tgccgtcggt ggtggaaaag gcgcgccggg ccgggttcgg gaag
//
LOCUS       DASTJJ010000876         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1831049, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000876 DASTJJ010000000
VERSION     DASTJJ010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831049"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFR02_10595"
     CDS             complement(<1..251)
                     /locus_tag="VFR02_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870927.1"
                     /translation="MMGCRSSLQPTKLLALLLWMGMPAFVWANPHIQILGESTTNPSL
                     GSTMYVTVAFCDDTSYANNRVKLMAAIISGSGQTSLASN"
     gene            complement(502..1548)
                     /locus_tag="VFR02_10600"
     CDS             complement(502..1548)
                     /locus_tag="VFR02_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9870928.1"
                     /translation="MPIQAPNRILFIRLKAIGDVVLCTPLVRALRKAYPAARLDLLTD
                     AAPASLLKHNPHLTTLLTAPPKGASLGGQWRFLKGLRQNRYDLVFDLFGNPRSAWMTF
                     LSGAPARAGYAYRGRKWAYTHRVPMNRTRKYQVEVNLDVLRYLGIPDDGIGTEIFLTD
                     GEKAWGESCLDGLGLQKGGGPRVGLNPTGTWSAKRWPAASWRELAQALSQTGIRPLLL
                     TGPGDGALADEVTRGLGERVALAPPTDLRQSASLIAGLDLLIGNDGAPQHIAQALGTR
                     SLTLFGPTWGISWNKPGDPRHRLMQDPPPCGPCDRTLCPNPPHPASGVPSYLECLTRL
                     TPEKVLEAARPFLA"
BASE COUNT          319 a          494 c          541 g          296 t
ORIGIN      
        1 cagttgctgg ccaaggaggt ctggccgctg cccgagatga tggcggccat gagcttgacc
       61 cggttgttgg cgtaggaggt gtcgtcgcag aaggccaccg tgacgtacat ggtcgaaccc
      121 aaggatgggt tggtggtgga ctcgcccagg atctggatat ggggatttgc ccaaacgaag
      181 gccggcatcc ccatccaaag aaggagcgcc agcagtttcg tgggttgtaa ggatgagcgg
      241 catcccatca tggattcccc tggtaacgtt ttaaatccgg gacatagaaa tgcattttcc
      301 ctgccaagcc taacggtcaa ttcccttaaa aatgaagtgt tttaacaata aaaccttgag
      361 ggaaaacctt tatatccaca aattagtcac ttttccatca agaaagtcaa aaaatacctt
      421 attttcacat tcaaactagg tcttttgggg ctgtcttacc agtgggcggg agggcttggc
      481 tattccaacg aaacggacgg atcaggccag aaatggccgg gccgcttcca acaccttttc
      541 cggggtcaag cgggtcaggc attccagata gcttgggacc ccggaagcgg ggtgcggcgg
      601 gttggggcaa agggtccggt cgcagggccc acaggggggc gggtcctgca tcaaacggtg
      661 ccggggatcc ccgggcttat tccaagaaat gccccaggtg ggcccgaaga gggtcaggct
      721 gcgcgtgccc aaggcctggg cgatatgctg gggcgccccg tcgttcccga tgagcaggtc
      781 caatccggcg atcagggaag ccgattggcg caggtccgtc gggggcgcca aggccacgcg
      841 ctcccctagg ccccgggtga cctcatccgc caatgcccca tcccccggcc cggtcaacag
      901 caggggccgg atgccggtct gtgaaagcgc ctgggccagt tcccgccagg aagcggcggg
      961 ccaccgcttg gccgaccaag tccccgtggg gttcaacccc acccggggac cgccgccctt
     1021 ctggagtccc aacccgtcca ggcaggattc gccccaggcc ttctccccgt cggtcaggaa
     1081 aatttccgtc ccgatcccgt cgtccgggat gcccaagtac cgcaggacgt ccaggttcac
     1141 ctccacctgg tattttctcg tccggttcat gggcacccgg tgggtgtagg cccacttgcg
     1201 gccccggtag gcgtagcccg cgcgcgcggg ggcgcccgag aggaacgtca tccaggcgct
     1261 gcggggattg ccgaagaggt cgaacaccag gtcataacgg ttctgccgga ggcccttcag
     1321 gaaacgccat tgcccgccga gggaagcccc cttcggcggg gccgtgagca gggtcgtcaa
     1381 atgggggttg tgcttcaaga gggaggccgg cgccgcgtcg gtcaaaagat cgagacgggc
     1441 ggcaggatag gctttgcgca gggcgcgcac caggggcgtg caaagcacca cgtccccgat
     1501 ggccttcagc cggatgaaga ggatcctatt gggggcttgg atcggcatga ggggctcgct
     1561 tgtcaggggg tctccgggca ccgagaacct tcaccatgcc ccggccaagg ggccggaccg
     1621 ggccgcggtc atttactttc gcggcccgtc
//
LOCUS       DASTJJ010000877         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_978060, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000877 DASTJJ010000000
VERSION     DASTJJ010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978060"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFR02_10605"
     CDS             <1..213
                     /locus_tag="VFR02_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870929.1"
                     /translation="SIPKTVMTLLKLRFNEKVKLEIATNNEVRIFSINITSGAEIKNK
                     KVTDYLKRKSSDLITVTISKNLARLT"
     gene            complement(263..1114)
                     /locus_tag="VFR02_10610"
     CDS             complement(263..1114)
                     /locus_tag="VFR02_10610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9870930.1"
                     /translation="MPDQPAGPESSAVRTALWRALHLQVDAPPFIIEDEVGLELAAPP
                     ADWRSRQDMHPGFTKVFRASIVARARFIEDLVTEQAARGVGQYVLLGAGLDSFAQRRP
                     DLASRLKVFEVDQPGPQAWKRARLAELGFGLPDWLRFVPVDFEAGDSWLEKLKSAGFD
                     PARPALVASTGVSLYLTKEAVTATLRQAAGLAPGSTLAMTFILPRDMASPEERPGYEA
                     AERGARAAGTPFLSYFMPDQMLSMAREAGLKDARHVSGKDLAARYFSGRTDGLKPGSE
                     SLLVAGV"
     gene            1345..2337
                     /locus_tag="VFR02_10615"
     CDS             1345..2337
                     /locus_tag="VFR02_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9870931.1"
                     /translation="MGQSVARRMRALYFEKTGGLEQLQVASRPWPELAPGDALIRVHA
                     AAINPSDVKNVLGRFSLTTLPRVPGRDFSGAVLKGPPDLVGREVFGSAPGLGFTRDGS
                     HAEVLAVPADCLALKPSLISFEQAAALGVPYLTAWQSLFAAGGLKEGETLLILGAGGA
                     VGSAAAHLAKTKRARVLGTATQASGLERVKPLPVDHWIALDQEDLVEKVMALTEGRGV
                     DLVLDAVGAPLFERGLKCLAQRGRQVSIASTASPRVEFHLVDFYHRESTLKGVDSLKL
                     SFKETGDILRELARLVEREKVPPPAVTAVALEEAPAAYARIAEGAHREKTVIRF"
     gene            complement(2378..3034)
                     /locus_tag="VFR02_10620"
     CDS             complement(2378..3034)
                     /locus_tag="VFR02_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9870932.1"
                     /translation="MEQRADVFTGVHKGLRRGLLGLSQKLGSADWSDAQDRQAVEAEF
                     KDMVYFLREHAENEDKIQAPTLEEKAPGATRQMAEDHQRLEKQIDGLETDWEAAAKAG
                     FPSEACHRLYLSYNRFLSGYLAHLDMEEGPITEAVHRHFTDDEIKAMVGRIIAKTSPK
                     DMGMMLSYMIPGMNAQERLTFLSGVQATAPAPVFDGLKGLAQKVLAPRDWEKLAKGLA
                     "
     gene            complement(3095..>3326)
                     /locus_tag="VFR02_10625"
     CDS             complement(3095..>3326)
                     /locus_tag="VFR02_10625"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9870933.1"
                     /translation="QDIDLYASLLEKDKALQAPLRPGSGFWLQLVTGALTVNGAPLAT
                     GDAFYGEGEASLDIRAGKESHFLLFDLGPFPR"
BASE COUNT          590 a         1076 c         1051 g          609 t
ORIGIN      
        1 agtattccga aaaccgtcat gacccttcta aaattaagat tcaacgaaaa agtcaagcta
       61 gaaattgcaa ccaataatga agttagaatt ttctcgatca acatcacttc tggcgcggaa
      121 attaaaaaca aaaaagtcac cgattattta aagcgcaaat cttccgatct tatcacggta
      181 acaatttcta aaaatttagc tcggttaacc tgaaaattat ttcccgatcg ctttcgtcat
      241 catgcaaaaa aaccatgctc ctctacaccc ccgccaccag cagcgattcg gacccgggtt
      301 tgaggccgtc ggtgcggccg ctgaaatagc gcgccgccag gtccttgccc gagacgtgcc
      361 gggcatcctt caagccggcc tcacgggcca tggacaacat ctggtcgggc atgaaatagc
      421 tgaggaaggg cgtgccggcg gcccgggcgc cccgctcggc cgcctcatag ccgggccgtt
      481 cctccgggga agccatgtcc ctgggcagga tgaaggtcat ggccagggtg gaaccgggcg
      541 ccagcccggc cgcctggcgc agggtggccg tcaccgcttc cttcgtgagg tacaggctga
      601 cgcccgtgga agccaccagg gccggtcggg ccgggtcgaa cccggcgctt ttgagttttt
      661 ccaaccacga atcccccgcc tcaaaatcga ccggcacgaa cctcaaccag tcgggcaggc
      721 cgaatcccag ttccgccagg cgggcgcgtt tccaggcctg ggggccgggt tggtcgacct
      781 cgaagacctt caggcgggag gcaaggtcgg gacggcgttg ggcaaagctg tccaaccccg
      841 cgcccaaaag cacatactgg cccacccccc gggcggcttg ttccgtcacc aggtcctcga
      901 tgaaacgggc ccgggccacg atggaggcgc ggaagacctt ggtgaaaccc ggatgcatgt
      961 cctggcgcga gcgccaatcc gcggggggcg cggccagttc cagccccacc tcatcctcga
     1021 tgatgaaggg gggcgcgtcg acctgaagat ggagcgcgcg ccaaagggcg gtgcgcaccg
     1081 ccgaactttc gggtcctgct ggttggtcag gcatgggggc tcccgttgtt tttagggtct
     1141 gtttcaaaac cctgaaaagt gcgatctttc acctcggaga cgcggggaaa aacgcatgcg
     1201 gggaccccgg cttttgacca tggtcctcac ttcgcgtctt tgcggtaaaa gaggttttga
     1261 aacggcgtct tagactatat cccaacccgg cggcgctgat aaccacccct gccttcccat
     1321 ccggcttttc cctataatcc ccccatggga caaagcgtcg cccgccggat gcgggccctt
     1381 tatttcgaga agaccggcgg cctggaacag ttgcaggtcg cttcccgccc ctggcccgag
     1441 ttggcgccgg gggacgccct gatccgcgtg cacgccgccg ccatcaaccc cagcgacgtg
     1501 aagaacgtcc tgggccgttt ttccctgacc actctgcccc gcgtgccggg gcgggatttt
     1561 tccggggcgg tcttgaaggg cccgcccgac ctggtgggac gggaggtctt cggctccgcc
     1621 cccggcttgg gcttcacccg ggacggttcc cacgccgagg tcctcgccgt gcccgccgat
     1681 tgcctggcct tgaagccttc cctcatctct ttcgagcagg ccgccgccct gggcgtgccc
     1741 tacctgacgg cctggcagtc gctgttcgcc gccggcgggc tgaaggaagg ggagaccctg
     1801 ctcatcctgg gcgccggcgg cgcggtgggg tccgcggcgg cccatttggc gaagaccaaa
     1861 agggccaggg tgctgggcac ggccacccaa gcctcggggc tggagcgggt gaagcccctt
     1921 ccggtggacc attggatcgc gctggaccaa gaggacctgg tggaaaaggt gatggccttg
     1981 accgaaggcc ggggcgtgga cctggtcttg gacgccgtgg gcgcccccct gttcgagcgg
     2041 ggcttgaaat gcctggccca acggggacgg caggtctcga tcgcctccac cgcctcgccc
     2101 cgggtggagt tccacctggt cgacttttac caccgggaat cgaccctgaa gggcgtggat
     2161 tcgctgaaat tgtccttcaa ggagaccggc gatattttgc gggagctggc ccgtctggtc
     2221 gaacgcgaaa aggtcccgcc cccggccgtg acggcggtcg ccttggagga agccccggcg
     2281 gcctacgcgc gcatcgccga aggcgcccac cgggaaaaga ccgtcatccg gttctgaggc
     2341 tttttcccgg cttccaggga acgggagaca gaccccctca ggccagcccc ttcgcgagct
     2401 tctcccaatc cctgggcgcc agcacctttt gggccaggcc cttgaggccg tcgaagaccg
     2461 gcgccggtgc ggtggcctgg acgcccgaaa ggaaggtgag ccgctcctgc gcgttcatgc
     2521 cggggatcat ataggaaagc atcatgccca tgtccttcgg ggaggtcttg gcgatgatgc
     2581 ggcccaccat ggccttgatc tcgtcgtcgg tgaaatgccg gtggaccgcc tcggtgatgg
     2641 gcccctcttc catgtccagg tgggccagat aaccggatag gaagcggttg tagctcaggt
     2701 aaaggcggtg gcaggcctca gagggaaatc cggccttcgc ggccgcttcc caatccgtct
     2761 ccaagccgtc gatctgtttt tccaggcgct ggtggtcttc ggccatctgc ctggtcgcgc
     2821 cgggggcctt ttcttcgagt gtgggcgcct ggatcttgtc ctcgttctcg gcgtgttccc
     2881 gcagaaaata gaccatgtcc ttgaactcgg cctcgacggc ctgacggtcc tgcgcgtccg
     2941 accagtccgc gctgcccagc ttttgggaaa gccccaaaag cccccggcga agccccttgt
     3001 gcaccccggt gaaaacatcc gcccgttgtt ccatgcgtcc cctccccgaa taggatggcg
     3061 cattcttcca tcccggcggc ccgggggcaa ggggtcagcg ggggaagggc cccaggtcga
     3121 agagcaggaa atgggattct ttcccggccc ggatgtccaa ggacgcctcg ccttcgccgt
     3181 agaaggcgtc gcccgtggcc aagggggcgc cgttcacggt gagcgccccc gtcaccaact
     3241 gcagccaaaa gcccgaaccc ggccggaggg gcgcttggag ggccttgtcc ttttccaaca
     3301 aggaggcgta gaggtcgatg tcctgg
//
LOCUS       DASTJJ010000878         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_607982, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000878 DASTJJ010000000
VERSION     DASTJJ010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607982"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>2132)
                     /locus_tag="VFR02_10630"
     CDS             complement(25..>2132)
                     /locus_tag="VFR02_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET9870934.1"
                     /translation="MSPSAEYTVQRELHYAIVDEVDSILVDEARTPLIISGPSEESTE
                     LYYKIDRIIPALKKGKEADYDVEEKTRSVFLTEAGVSHVEKLLKVDNLYDHKNVQLVH
                     HVQQALKAHVIFKRDVDYIVKDGEVVIVDEFTGRLMPGRRYSDGLHQALEAKERVKIE
                     AENQTLATITFQNFFRMYKKLSGMTGTAETEAAEFKKIYNLDVVGIPTHRPMVRLDDG
                     DAVYRTEREKFEAIADEVAALHGKGQPVLVGTVSIEKNETLSALLKKRGVRHEVLNAK
                     NHEREAEIISQAGQPGAVTIATNMAGRGTDIVLGEGMKDKGGLAVLGTERHESRRIDN
                     QLRGRSGRQGDPGLSRFYVSLEDDLMRIFGSDRLKNLMTSLGMEEGEVISHPWVSKAI
                     ERAQKAVENHNFDIRKHLIEYDDVMNKQREVIYRQRNRVLREKDVRAHMLKVLDDLAV
                     MGVGNFAPEKDQATWDLEGLTKWFKDIFRGDLTWDEAKTRMMDREALYEALKREILLA
                     YEAKEKAMTPEVMRTLERAISLQVVDGLWKEHLLAMDHLKEGIGLQAYGQKDPLIEYK
                     RQGFALFDELQDRVEEESIRLIFMVQKVDPAEDLFAAATPPQKVREEHPSFDPRAAAP
                     KPAPAAEGPAALMGQNAPPMGAPPLLGGQPWETQGQAQPQGREAVKLMPMKRDEPKVG
                     RNDPCPCGSGKKYKKCHGA"
BASE COUNT          274 a          712 c          705 g          441 t
ORIGIN      
        1 ggtcgaccca tcaggcctcg tccatcaggc cccgtggcac ttcttatact tcttccccga
       61 cccgcagggg caggggtcgt tgcggcccac tttgggttcg tcgcgcttca tcggcatcag
      121 cttgacggct tcccggccct ggggctgggc ctggccctgg gtctcccagg gttggccgcc
      181 cagcaggggg ggcgcgccca tcgggggggc gttctggccc atgagggccg cggggccctc
      241 ggcggcgggc gcgggtttgg gcgcggcggc gcgggggtcg aaactgggat gctcttcccg
      301 gaccttctgg ggcggcgtgg cggccgcgaa gaggtcctcg gcggggtcca ccttttggac
      361 catgaagatg agccggatcg attcctcctc cacccggtcc tgcagctcgt cgaagagggc
      421 gaagccctgg cgcttgtact cgatcaaggg gtccttctgg ccgtaggcct gcaggccgat
      481 gccttccttc aggtggtcca tggccaacag gtgttccttc caaagcccgt ccaccacctg
      541 cagcgagatg gcccgctcca gggtgcgcat cacctcgggc gtcatggcct tttccttggc
      601 ctcataggcc agcaggatct cccgcttgag ggcctcgtag agggcctccc ggtccatcat
      661 gcgggtcttg gcctcgtccc aggtgaggtc gccccggaag atgtccttga accacttggt
      721 caggccctcc aggtcccagg tggcctggtc cttctcgggc gcgaaattgc ccacgcccat
      781 caccgccagg tcgtccaaca ccttcaacat gtgggcgcgg acgtccttct cgcgcagcac
      841 gcggttgcgc tggcggtaga tgacctcgcg ctgcttgttc atcacgtcgt cgtactcgat
      901 gaggtgcttg cggatgtcga agttgtggtt ctccaccgcc ttctgggccc gctcgatggc
      961 cttggacacc caggggtgcg agatgacctc gccctcttcc atgcccaggc tggtcatgag
     1021 gttcttgagc cggtccgacc cgaagatgcg catcaggtcg tcctccagcg acacgtagaa
     1081 gcgggacaat cccgggtcgc cctggcgccc ggaccggccg cgcagctggt tgtcgatgcg
     1141 gcggctctcg tgccgctcgg tgcccaggac cgccaggccg cccttgtcct tcatgccctc
     1201 gcccagcacg atgtccgtgc cgcggcccgc catgttggtg gcgatggtga ccgccccggg
     1261 ctggcccgcc tgggagatga tctcggcctc ccgctcgtgg ttcttggcgt tcaggacctc
     1321 gtgccggacg ccccgcttct tgagcagggc cgacagggtc tcgttcttct cgatggacac
     1381 ggtccccacc agcacgggct ggcccttgcc gtgcagcgcc gccacctcgt cggcgatggc
     1441 ctcgaacttc tcccgctcgg tgcggtagac ggcgtcgccg tcgtccaggc gcaccatggg
     1501 gcggtgggtg gggatgccca ccacgtccag gttgtagatc ttcttgaact cggcggcctc
     1561 ggtctcggcg gtgcccgtca tgccggacag cttcttgtac atgcggaaga agttctggaa
     1621 ggtgatggtg gccagggtct ggttctcggc ctcgatcttg acccgctcct tggcctccag
     1681 ggcctggtgc aggccgtccg agtagcggcg gcccggcatg agccggcccg tgaactcgtc
     1741 cacgatcacc acttccccgt ccttcacgat gtagtccacg tcccgcttga agatgacgtg
     1801 ggccttgagg gcctgctgca cgtggtgcac cagctgcacg ttcttatggt cgtagaggtt
     1861 gtccaccttg agcagctttt ccacgtggga aacgcccgcc tcggtcagga agaccgaccg
     1921 ggtcttttcc tccacgtcgt agtcggcctc cttgcccttc ttgagggccg ggatgatgcg
     1981 gtcgatcttg tagtagagct cggtggattc ctcgcttggc ccggagatga tgagcggggt
     2041 gcgggcctcg tccaccagga tgctgtccac ctcgtccacg atggcgtagt gcagctcgcg
     2101 ctgcacggtg tattcggcgc tgggggacat gt
//
LOCUS       DASTJJ010000879         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1464719, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000879 DASTJJ010000000
VERSION     DASTJJ010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464719"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1251)
                     /gene="bamA"
                     /locus_tag="VFR02_10635"
     CDS             complement(<1..>1251)
                     /gene="bamA"
                     /locus_tag="VFR02_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET9870935.1"
                     /translation="LVLNQSRAWRWAAVLLISAAAAPAFSAADSPALPKIEKINVEGN
                     HNTSALVIYGHLSEREGDDFSLRKVRDDIHNLFAMGDFKDVQADAEPGTRDNQIVLTF
                     KVVERPLVSKITFNGNRKWGASKFLETIKTAPKTPFDPSQANADAAAIRALYLDEGYA
                     SATVAYQTHLDADANTVEVEFDIVEGLSIKVGPIEVTGAQAFSAKKVADQMKDIHKGD
                     KYKPEKLEEDLQQIEDFYHNEGYLRAAVVSHSERIDQATRLVYISLTVREGLQYSVGK
                     VQFDGNSLFDDAKMQQALGLKSGDLLRQDRLDDGLRAMRSLYADEGYIYANVAQHLDY
                     HDDTRKVDLDFTVNEGSVAHVQDVKIVGNYKTRDYVIRRELAIHPGDKFAADKIRESA
                     QNLYNLGYFEEVNPEVEPGDSPGK"
BASE COUNT          192 a          385 c          406 g          268 t
ORIGIN      
        1 ccttgcccgg cgaatcgccc ggctcgacct cggggttcac ttcctcgaaa tagcccaggt
       61 tgtagaggtt ctgggcggac tcgcggatct tgtcggcggc gaacttatcc cccggatgga
      121 tggccagttc ccggcggatg acgtagtccc gggtcttata gttccccacg atcttcacgt
      181 cctgcacgtg ggccaccgac ccttcgttca ccgtgaagtc caggtccacc ttgcgggtgt
      241 cgtcgtggta gtccaggtgc tgggccacgt tggcgtagat gtatccctcg tcggcgtaga
      301 ggctgcgcat ggcccgcaag ccgtcatcca agcggtcctg ccgcagcagg tccccgctct
      361 tgaggcccag ggcctgctgc atcttggcgt cgtcgaaaag cgagttcccg tcgaactgca
      421 ccttgcccac gctgtactgc aggccttccc gcacggtcag gctgatgtag accagccgcg
      481 tggcctggtc gatgcgctcg ctgtgcgaga ccacggcggc ccgcaggtag ccctcgttat
      541 ggtagaaatc ctcgatctgc tgcaggtcct cttccagttt ctcgggtttg tacttgtccc
      601 ctttgtggat gtccttcatc tggtcggcca ccttcttggc gctgaaggcc tgggcccccg
      661 tcacctcgat gggacccact ttgatggaaa gcccttccac gatgtcgaac tcgacctcga
      721 cggtgttggc gtcggcgtcc aggtgcgtct ggtaggccac cgtggccgag gcgtagccct
      781 catccaggta cagcgcccgg atggcggccg cgtcggcgtt ggcttgggag gggtcaaagg
      841 gcgtcttggg ggcggtcttg atggtctcca ggaatttgga ggccccccac ttgcggttcc
      901 cgttgaaggt gatcttggac accagggggc gttccaccac cttgaaggtc aggacgatct
      961 ggttgtcccg ggtgccgggc tcggcgtccg cttggacgtc cttgaagtcg cccatggcaa
     1021 aaagattgtg gatgtcgtca cggaccttgc gcagggagaa atcgtccccc tcccgctcgg
     1081 ataggtggcc gtagatcacc agggcggaag tattgtggtt gccctcaacg ttgatcttct
     1141 cgatcttggg gagcgcgggg gaatccgccg ccgaaaaggc gggagcggcc gccgccgaga
     1201 tcaaaagaac ggctgcccag cgccaagcgc gggattggtt gagcacgagc t
//
LOCUS       DASTJJ010000880         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1831452, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000880 DASTJJ010000000
VERSION     DASTJJ010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831452"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFR02_10640"
     CDS             <1..264
                     /locus_tag="VFR02_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9870936.1"
                     /translation="ILGIRPEDIHDKMDAANASPDNTISATVEIIEPLGAEAYLYLTT
                     GKSPFIARVSNHDQIEINQDIELVFNMQKAHIFDKETEKCLTN"
     gene            353..1657
                     /locus_tag="VFR02_10645"
     CDS             353..1657
                     /locus_tag="VFR02_10645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9870937.1"
                     /translation="MNIAVVGTGYVGLVTGSCFADLGNQVTCVDVDARKVRELRRGVV
                     PIFEPGLEDIVRRNKGSRLSFTTDLAGAVRDSDFIFIAVNTPPNRRGEADISNVEAVA
                     RGIARSINGYKIIINKSTVPIGMGDIVGRILRRFGPKGAAFDVVSNPEFLREGSAVTD
                     LLKPERVVIGASRKEAAHQVAEIYKPLKCPILITDLKSAEMIKYASNAFLAAKISFVN
                     EIANLCDQVGADILQVTQGMSYDGRIGGQFLNAGLGFGGSCFPKDVSALVQIGKHNRY
                     SFRMLPKVLEVNQYQRRYFLKKVAKAAGPVRGKTFAVWGLSFKPNTDDLREAPSLTVV
                     PALLAQGARVRVYDPVAMPKARGLFPKARFCKDAYEAARGADAVLVLTDWNEFKQVDL
                     GKLRSLVKRPILLDGRNMYDAREMAEKGFAYQGMGRGAAAEA"
BASE COUNT          338 a          556 c          588 g          322 t
ORIGIN      
        1 atcctgggca tccggccgga ggacatccac gacaagatgg acgcggccaa cgcgtccccc
       61 gacaacacca tcagcgccac ggtggagatc atcgaacccc tgggcgccga ggcctacctg
      121 tacctgacga cggggaagag ccccttcatc gcgcgggtga gcaaccatga ccagatcgag
      181 atcaaccagg acatcgagtt ggtcttcaac atgcagaagg cccatatttt cgacaaggaa
      241 acggagaagt gcctgaccaa ttgacgtcgg gtacggaggc tttccgaaaa accgggacag
      301 cgatgccccg gttttttctt tatgacaacc actgcggggg gacggcgccg gtatgaacat
      361 cgcggtcgtg gggacgggat atgtgggctt ggtcaccggg tcctgcttcg ccgatctggg
      421 gaaccaggtc acttgtgtgg acgtggacgc gcgcaaggtg cgcgagttgc gccggggcgt
      481 ggtgcccatt tttgagccgg ggctggaaga catcgtccgg cgcaacaagg ggtcccggct
      541 ttccttcacc accgacttgg ccggggctgt ccgggattcg gatttcatct tcatcgcggt
      601 caataccccc cccaaccggc ggggtgaggc cgacatctcc aatgtggaag ccgtggcccg
      661 gggcatcgcc cgttccatca acggttacaa gatcatcatc aacaagagta cggtgcccat
      721 cggcatgggc gacatcgtcg gccggatcct ccggcgcttc ggccccaagg gggcggcctt
      781 tgacgtggtc tccaaccccg aattcctccg ggaggggtcc gcggtcaccg acctgttgaa
      841 acccgagcgc gtggtcatcg gcgcctcccg caaggaggcg gcccatcagg tggcggagat
      901 ctacaaaccg ctcaagtgcc cgatcctgat caccgacttg aagagcgccg aaatgatcaa
      961 gtacgcctcc aatgccttcc tggcggcgaa gatctccttc gtcaatgaga tcgcgaacct
     1021 ctgcgaccaa gtgggcgccg acatcctgca ggtcacccag ggcatgagct acgacgggcg
     1081 gatcggcggc caattcctga acgctgggtt ggggttcggg ggaagttgtt ttcccaagga
     1141 cgtttccgcc ctggtccaga tcggcaaaca caaccgctat tccttccgca tgttgcccaa
     1201 ggtattggag gtcaaccaat accagcgccg ctatttcctg aagaaggtcg ccaaggcggc
     1261 ggggcccgtc cgcggaaaga ccttcgcggt ctggggcttg tccttcaagc ccaacaccga
     1321 cgacctgcgc gaggcgccct ccctgaccgt ggtgccggcg cttttggccc agggggcccg
     1381 ggtgagggtc tatgacccgg tggccatgcc caaggcccgg ggcctgttcc ccaaggcccg
     1441 tttttgcaag gacgcctacg aagccgcgcg cggggcggac gcggtgctgg tgttgaccga
     1501 ttggaacgag ttcaagcagg tggacctggg gaaactccgg tccttggtga aacgacccat
     1561 ccttttggat gggcggaaca tgtacgatgc ccgggagatg gcggagaagg gtttcgccta
     1621 tcaagggatg gggcggggcg cggccgcgga agcctgaggc cccagcaaag tatcgggagc
     1681 tgtcctttgg aagtcgcctt actcggatcc accggcatgt tgggccgcca gatcgccgac
     1741 gccctccggc gcgcgggcca ttcggtgttg gcgcccgccc gcggggaggc ggacctttcc
     1801 cgtc
//
LOCUS       DASTJJ010000881         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_608219, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000881 DASTJJ010000000
VERSION     DASTJJ010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608219"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFR02_10650"
     CDS             complement(<1..573)
                     /locus_tag="VFR02_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9870938.1"
                     /translation="MLNSRSRATLKQIAKYCGVSHTTVSMVINQNPRISAETREKVLE
                     AIKKFNYYPNVAARSLVMSRTNTIGIFGTMFDSPYYNEIIRGIEMECRHANVDLKIYN
                     CNGSLEEFNDIYDRILGERRIDAVIALSVPMEDAVLERFQKEELPLIILQPVALEGPN
                     HLLEKIPTLQAEDRKGIYKATAYLISLGHKK"
     gene            complement(609..953)
                     /gene="secG"
                     /locus_tag="VFR02_10655"
     CDS             complement(609..953)
                     /gene="secG"
                     /locus_tag="VFR02_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET9870939.1"
                     /translation="MYTFILILFVLTALLLIGVVLIQQPKSAGGGLFAGTGQSLLGTS
                     GKTFWTKFTTILAALFMLLCLGLSILPRLQGPGSSVADSIMKQEKASQAAAGASAPQP
                     AAQPPAGTAKKP"
     gene            complement(1078..>1272)
                     /locus_tag="VFR02_10660"
     CDS             complement(1078..>1272)
                     /locus_tag="VFR02_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9870940.1"
                     /translation="VHAFIRAWIGSKYNAQAAEAMRIQYGGSVKPENIKQLMAQPDID
                     GALVGGASLKADSFEALVRF"
BASE COUNT          237 a          360 c          417 g          258 t
ORIGIN      
        1 cttcttgtgc cccagggaga tcaggtaggc ggtggccttg tagatgccct tgcggtcctc
       61 cgcctgcagg gtggggatct tctccaacag gtggttgggc ccttccagcg ccacgggctg
      121 caggatgatg aggggcagtt cttccttctg gaaacgctcc agcaccgcgt cctccatggg
      181 cacggacagg gcgatcaccg cgtcgatgcg gcgctccccc aggatgcggt cgtagatgtc
      241 gttgaactcc tccaggctgc cgttgcagtt gtagatcttc aggtccacgt tggcgtggcg
      301 gcattccatt tcgatgcccc ggatgatctc gttgtagtag ggcgagtcga acatggtgcc
      361 gaagatgccg atggtgttgg tgcgggacat gaccaggctg cgggcggcca cgttggggta
      421 atagttgaac ttcttgatgg cctccagcac cttttcccgg gtctcggccg agatgcgggg
      481 gttctggttg atgaccatgg acaccgtggt gtgggacaca ccgcagtact tggcgatctg
      541 tttcagggtg gcgcgggatc ggctgttgag catgatatcg ttttaaagac ctcggttaaa
      601 aaaacggctc aaggtttctt cgccgttccc gccggcggct gggcggcggg ttggggggcg
      661 gaggccccgg cggcggcctg cgacgccttc tcctgcttca tgatggaatc ggccaccgaa
      721 ctgccgggcc cctgcaagcg gggcaggatg gaaagcccca ggcaaagcag catgaagagg
      781 gcggccagga tggtggtgaa cttggtccaa aaggtcttgc cgctggtgcc caagaggctt
      841 tggcccgtgc cggcgaaaag cccccccccg gcgcttttgg gttgctggat caggaccacc
      901 ccgatcagca acaaggcggt cagcacgaac aggatcaaga taaaggtgta catggctctc
      961 ctatgggatc ggcgggacgg ggtttccggc gaaacctaaa aaggccggcc cagcccactt
     1021 ttgaaaccga aggtttattt taaattaaac cggggcgaaa atgccaaaaa aatagggtca
     1081 aaaacggacc agcgcctcga aggaatcggc cttgaggctg gcccccccca ccagggcccc
     1141 gtcgatgtcg ggctgggcca tcaattgctt gatattctcc ggcttcacgc tgcccccgta
     1201 ctggatccgc atggcctccg cggcctgggc gttgtacttc gacccgatcc aggcccggat
     1261 gaaagcgtgg ac
//
LOCUS       DASTJJ010000882         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1344071, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000882 DASTJJ010000000
VERSION     DASTJJ010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344071"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFR02_10665"
     CDS             <1..290
                     /locus_tag="VFR02_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870941.1"
                     /translation="LKMHLEEAFSGLKAYLGEGIQGGSGGAEMNRNHGKGFDGGRASG
                     KRGHPSRHLHRRWSGVKTNDRRAKKSCTICLDTRNGRFLSDPSGRINHQRA"
     gene            297..>1469
                     /locus_tag="VFR02_10670"
     CDS             297..>1469
                     /locus_tag="VFR02_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870942.1"
                     /translation="MRNELSDLPMNRSRTLPWKALGPGPQEPPFEFCLSSVFQGLLAF
                     LPIARIWFDLVQLKPDNKTPQYEAYLLSWILLPLLGILLTRFLGRFRPGGIPPHIYLR
                     TSVFLNFILLLAAYWDIQRPDPAPVLFWAALYLLTQAAAWTWASGWGRSPYWRRPSFS
                     AWIYPAMAVLFLGWLTRIDPAWEGRLRPWHLANWLFAAALAYAFLVDLDPRSVRARWG
                     TSSRAWGLVLTVLLTAFFAFLFVDPTGSDCPYNQGYYLGPLADFVNGKTLLVDINSQY
                     GVLLFFFLRLFFPAALPLGFHSFFFFDALLRLCQYVVFFWIARKLFKSILYPALGTVV
                     LILLNCGLGPTSALWYPSQGALRFGFIFLLAALVVLRNRHPRWAAWFLGLESATAAV"
BASE COUNT          206 a          514 c          413 g          336 t
ORIGIN      
        1 ctctcaaaat gcatttggag gaggcgtttt cgggcttgaa ggcgtatctg ggcgaaggga
       61 tccaaggcgg ctccggcggg gctgaaatga accgaaacca tggtaaaggt tttgacgggg
      121 gaagggcaag cgggaaaagg ggccacccgt cccgtcatct tcatcggcgg tggagcgggg
      181 tgaaaaccaa tgaccgacgg gcgaagaaat cctgtaccat ttgcctggat acccggaacg
      241 gccgtttttt gtccgaccca tccggccgga tcaaccacca gcgcgcgtga aagaccttga
      301 ggaacgaact ctccgacttg cccatgaacc ggtcccggac ccttccctgg aaggcccttg
      361 ggcccgggcc ccaggaaccc ccgttcgaat tctgcctttc gagcgttttc cagggcctgc
      421 tggccttcct gcccatcgcc cgcatctggt tcgatctggt ccaattgaag cccgacaata
      481 agaccccgca atatgaggcc tacctgttga gctggatcct gcttcccctc ctggggatcc
      541 tgctcacccg gttcctgggg cgtttccggc ccggggggat cccgcctcat atttacctgc
      601 ggacttccgt cttcctgaac ttcatcctgc tgttggcggc ctattgggac atccaacggc
      661 cggaccctgc cccggtcctc ttctgggccg ccctctacct cctgacccaa gccgccgcct
      721 ggacctgggc ctccggctgg ggccggtccc cctactggag gcggccgtcc ttctccgcct
      781 ggatctatcc ggccatggcg gtccttttcc tgggctggct cacccggatc gaccccgctt
      841 gggagggccg gctccgaccc tggcaccttg ccaactggct tttcgccgcc gccctggcct
      901 acgcctttct cgtggacctg gatccccggt ccgtccgggc ccgctggggg acaagttccc
      961 gggcctgggg cctggtcctc accgtcctcc tgaccgcctt tttcgccttc ctcttcgtgg
     1021 accccaccgg cagcgattgt ccctacaacc agggctacta cctcggaccg ctggcggatt
     1081 tcgtgaacgg gaagaccctg ctggtcgaca tcaactccca gtacggcgtc cttcttttct
     1141 ttttcctaag gctctttttc ccggccgcgc tccccctggg ctttcattct ttcttttttt
     1201 tcgacgcctt gctgcggctt tgccaatatg tcgtcttctt ctggatcgcc cgcaaactgt
     1261 tcaagtccat cctctacccg gcgctgggga cggtggtcct gatcctactc aattgcggcc
     1321 tgggcccgac cagcgccctc tggtaccctt cccaaggcgc gctgcggttc ggtttcatct
     1381 tcttgttggc cgccctggtg gtcctgcgca accgccatcc ccgatgggcg gcctggttcc
     1441 tcgggctcga gtcggccacg gccgccgtg
//
LOCUS       DASTJJ010000883         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_609106, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000883 DASTJJ010000000
VERSION     DASTJJ010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609106"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..112
                     /locus_tag="VFR02_10675"
     CDS             14..112
                     /locus_tag="VFR02_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET9870943.1"
                     /translation="MRQGDWRIIYRVDDGNKAVVIVKIGHRRDVYR"
     gene            complement(141..524)
                     /locus_tag="VFR02_10680"
     CDS             complement(141..524)
                     /locus_tag="VFR02_10680"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9870944.1"
                     /translation="MAKEAVPKTKGFLEHRAHHRVPVKVPVQFQVLDKREEVDKLQHA
                     KGVTKDISLQGLFLRTAQDVKPGDVFRLEIPLPDQKRALFAYAEVVWVRSGKMFFKND
                     AGLKLMLMPVEDQEALKAYLDAAAK"
     gene            complement(527..706)
                     /locus_tag="VFR02_10685"
     CDS             complement(527..706)
                     /locus_tag="VFR02_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870945.1"
                     /translation="MKNVEMKVEGNILTLRVDLSKEFGPSASGKTIIVATTEGNVSVP
                     DRDEKVGLNVYKKKA"
     gene            complement(724..1008)
                     /locus_tag="VFR02_10690"
     CDS             complement(724..1008)
                     /locus_tag="VFR02_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870946.1"
                     /translation="MKLCHACKTPWDGAPGTQPGRNETCSRCGADLHVCLNCRLYDPA
                     CANECRSSTTERVRDKDKRNFCDEFELAARAPGGSDPLEDRKKKWDDLFQ"
     gene            complement(1149..>1721)
                     /locus_tag="VFR02_10695"
     CDS             complement(1149..>1721)
                     /locus_tag="VFR02_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870947.1"
                     /translation="QAMGSAYYAEGDKPDALNAFNQSLAAHPDPQLQQFADSLRRSAA
                     TPSAASHPAAENPGPVVGGGNFGIGLEAGEPGSYGASGMYWLDQQSSFQGEVKLNGGA
                     LFQFAYLWHDFDLIRAKNGAFAFYVGIGGDAAVNGVLAAAVEAPVGITYFFQRSQVPI
                     DLYIQAEPTFWVTPGTEFMIFGNLGARYYF"
BASE COUNT          285 a          537 c          553 g          346 t
ORIGIN      
        1 ggaccgctac cgcatccggc aaggggattg gcggatcatc tatcgggttg atgatgggaa
       61 caaggccgtc gtgatcgtca agatcggcca ccgtcgcgac gtctatcgat aagacgatcc
      121 atcgttgtga ttcaagggaa ctatttcgcc gccgcgtcca ggtaggcctt caacgcctct
      181 tggtcctcga cgggcatgag catgagcttg agaccggcgt cgttcttgaa gaacatcttg
      241 cccgatctca cccagaccac ctcggcgtag gcgaagaggg cccgcttctg gtcgggcagg
      301 gggatctcca accggaagac gtcgccgggc ttgacgtcct gggccgtgcg caggaacagg
      361 ccctgcaggc tgatgtcctt ggtgacgccc ttggcgtgct gcagcttgtc cacttcctcg
      421 cgcttgtcca gcacctggaa ctgcacgggc accttcacgg gcacccggtg gtgggcccgg
      481 tgctccagga aacctttggt cttcgggacg gcttctttcg ccaaggtcag gccttctttt
      541 tgtagacgtt caatccgacc ttctcatccc ggtcgggcac ggacacgttc ccctcggtgg
      601 tggcgacgat gatcgtcttg cccgaggccg aggggccgaa ttccttggag aggtcgaccc
      661 tcagggtcag gatgttccct tccaccttca tttcgacgtt cttcacggcg ggcctccgca
      721 ggtttactgg aacaaatcat cccatttctt cttgcggtcc tcaagggggt cggacccccc
      781 gggggcccgg gccgccagct cgaattcatc gcaaaagttg cgtttgtcct tgtcccgcac
      841 ccgctcggtg gtggaacttc ggcattcgtt ggcgcaggcc gggtcgtaca ggcggcagtt
      901 gaggcaaaca tgcaggtcgg ccccgcaacg ggaacaggtc tcgttgcgtc ccggctgggt
      961 gccgggcgcc ccgtcccaag gggtcttgca ggcgtggcaa agcttcattc caggttcccc
     1021 tgggcccatt tcaccacccg aacggcatat attacgttga cgatcggtgc caaagatcct
     1081 tttccttgga cgtccccatc agcccttggc gtcttggcgc ctttgcggtg aaaaggccct
     1141 tgggcgtctt aaaaataata ccgggcgccc aggttgccga agatcatgaa ttccgtgccc
     1201 ggggtcaccc aaaaggtggg ttcggcctgg atgtagaggt cgatggggac ctgggagcgc
     1261 tggaagaaat aggtgatgcc caccggcgcc tcgacggcgg ccgccaggac cccgttgacc
     1321 gccgcgtccc cgccgatgcc gacgtaaaag gcgaaggccc cgttcttggc gcggatcagg
     1381 tcgaaatcgt gccacaggta ggcgaattgg aagagcgcgc ccccgttgag cttcacctcg
     1441 ccctggaagg aactttgctg gtccagccaa tacatgccgc tggcgccgta gctgccgggt
     1501 tcgcccgcct ccagcccgat gccgaagttg cccccgccca ccacgggccc ggggttttcg
     1561 gcggcgggat gggacgcggc cgaaggcgtg gcggcggacc gccgcagcga atcggcgaat
     1621 tgctgcaact ggggatcggg gtgcgccgcc aggctctggt tgaaggcgtt caaggcgtcg
     1681 ggcttgtcgc cctcggcgta gtaggcgctg cccatggcct g
//
LOCUS       DASTJJ010000884         1848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_853904, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000884 DASTJJ010000000
VERSION     DASTJJ010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853904"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFR02_10700"
     CDS             <1..239
                     /locus_tag="VFR02_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9870948.1"
                     /translation="AEAAGEYLKKGDPIFVEGRLQSKSWETEDGQKRSSMEVVADRVQ
                     FLNRGKGGDTPLHGVDEEAAGPAAAGASEEDIPF"
     gene            304..705
                     /gene="rpsR"
                     /locus_tag="VFR02_10705"
     CDS             304..705
                     /gene="rpsR"
                     /locus_tag="VFR02_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9870949.1"
                     /translation="MEENKSTTTPASVPAAPAAPAAPAAPSAPTTSTSPAPSSGPHRS
                     GPHSGPRPPREGGGPGGRFRRPRRKSCRFCAEKVVAIDYKDYNRLRSFVSERGKIVPS
                     RMTGTCASHQRELTRAINRARNIALLPFANN"
     gene            719..1483
                     /locus_tag="VFR02_10710"
     CDS             719..1483
                     /locus_tag="VFR02_10710"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HET9870950.1"
                     /translation="MALPILGLLLDLALVTVILLVAGVTAVWALLKGMSSRGVVLVSA
                     LAAFAFLGAYFAVILPGFHEFFTEALKAYEAVWTAQTPSLLKSGASQENIDLFKAFAL
                     KYFLYAFPAWVAVGSLTAGLVSYYLASALLRRVTARVQRPMPFRLWVLPDVMVFGFIA
                     GCFLKLFGPENSAWDVVGDNLVVFFAVFYTLGGLSIVSFFLNKWGLTVFWRVVVYILM
                     LQLSLEVMVPVASLGLMDLWFDFRKLRAAPAPGGKP"
     gene            1480..>1848
                     /gene="rplI"
                     /locus_tag="VFR02_10715"
     CDS             1480..>1848
                     /gene="rplI"
                     /locus_tag="VFR02_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9870951.1"
                     /translation="MKVLLTQDVKKIGKKGEVLEVKEGYARNFLIPKGLAVEASGGAM
                     KQHQDGQKAQEKRQAREKEEARELAAKLSAAPVVLKHKAGEEGRLFGSITSAEIAEAL
                     KAKGFEVDKKKIVLEEPIRLV"
BASE COUNT          305 a          605 c          623 g          315 t
ORIGIN      
        1 aggccgaggc cgccggggag tacctgaaga agggcgaccc gatcttcgtg gagggccgcc
       61 ttcagagcaa gtcctgggag accgaggacg gccagaagcg cagctccatg gaagtggtgg
      121 cggaccgcgt gcagttcctc aaccggggca agggcggtga cacccccctg cacggcgtgg
      181 acgaggaagc ggccggaccg gccgcggccg gggccagcga ggaagacatc cccttttaag
      241 aaggctttat tcacccggcc gcccgtcggc cacatttgaa tgaacgatca aggagaaacg
      301 aaaatggaag agaacaagtc aacgaccacc ccggcgtccg tcccggccgc ccccgccgct
      361 cccgctgccc cggccgcccc gtcggccccc acgacctcca cgtccccggc gccctcgtcc
      421 ggaccgcacc ggtcgggccc gcattcgggc ccccgcccgc cccgcgaggg aggcggcccc
      481 ggcggccgtt tccgccgccc ccggcgcaag tcctgccgtt tctgcgccga gaaggtcgtg
      541 gccatcgact ataaggatta caaccggctg cgcagcttcg tcagcgagcg gggcaagatc
      601 gtccccagcc gcatgacggg gacctgcgcg tcccaccagc gggaactgac ccgggccatc
      661 aaccgggccc ggaacatcgc cctgctgcct ttcgccaaca actaaccggc gggccgagat
      721 ggctttgccg atcctgggcc tgctcctgga cctggcgctc gtgacggtga tcctcctggt
      781 cgccggcgtg accgccgttt gggcgctctt gaagggcatg tcgtcgcgcg gcgtggtgct
      841 ggtctcggcc ctggccgcct tcgccttcct gggggcatat ttcgccgtca tcctgccggg
      901 cttccacgaa ttcttcacgg aggccctgaa ggcctacgag gccgtctgga cggcgcagac
      961 gccttccttg ctgaagtccg gcgccagcca ggagaacatc gacctgttca aggccttcgc
     1021 cttgaagtat ttcctctacg ctttcccggc ttgggtggcg gtggggagct tgaccgcggg
     1081 gctggtttcc tattacctgg cctcggccct gttgcgccgg gtgaccgcgc gggtgcagcg
     1141 gcccatgccc ttccggctct gggtcctgcc cgacgtgatg gtcttcggtt tcatcgccgg
     1201 ctgtttcctc aagctcttcg gcccggagaa ctcggcctgg gacgtggtgg gggacaacct
     1261 ggtggtcttt ttcgcggtct tctacaccct gggggggctg agcatcgtct ccttcttcct
     1321 gaacaagtgg gggctgacgg tcttctggcg ggtggtggtc tatattctga tgctccaatt
     1381 gtccctggag gtgatggtgc cggtcgccag cctgggactg atggacctgt ggttcgattt
     1441 caggaagttg agggccgctc cggcccccgg gggaaaacca tgaaagtctt gctgacccag
     1501 gacgtgaaga agatcgggaa aaaaggcgag gtgttggagg tgaaggaggg ctatgcccgc
     1561 aacttcctca tccccaaggg cctggcggtg gaggcctcgg gcggcgccat gaagcagcac
     1621 caggacgggc aaaaggccca ggaaaagcgc caggcccggg aaaaggaaga ggcccgggaa
     1681 ttggccgcca agctctcggc cgcgcccgtc gtgctcaagc acaaggccgg cgaggaaggc
     1741 cgcctgttcg gctccatcac ctccgccgag atcgcggagg ccctgaaggc caagggcttc
     1801 gaggtggaca aaaagaagat cgtgctggag gagcccatcc ggctggtg
//
LOCUS       DASTJJ010000885         1388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1221815, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000885 DASTJJ010000000
VERSION     DASTJJ010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221815"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFR02_10720"
     CDS             complement(<1..877)
                     /locus_tag="VFR02_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9870952.1"
                     /translation="MPARLVLFAKTLLTLDRPQPLEDAFVLVEGSRILQVGRRKDFHA
                     PRPLRVLDLGDTLLLPGLINAHCHLDYTAFKGRVPFKGSFREWLREMAVKGRAATAAE
                     YHRSVQAGIRESLAQGTTTLCDISTSGESFKLLPGSGLRAFAFLELLDVAQPDPKAVW
                     KGFQERLKALTAAGEGRETFRWGISPHTPFTVSKELLLLAGRFAAAHPHCPTTIHVGE
                     SRDEGRFFRTGRGPMAKRIQALNPDWTLPHGTTPVGFLGAAGWLPKLNLALHVNVADA
                     RDLGLLARHRVAVVHC"
     gene            1013..>1388
                     /locus_tag="VFR02_10725"
     CDS             1013..>1388
                     /locus_tag="VFR02_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870953.1"
                     /translation="MLDNKFQKFLFHLTSLATTAFPRGGAWFFNHTIPPILGNRALVD
                     WSFRRNLAALRKVQKFERILVIPDVHIGDAIMMQAAVTAFRDFFPQARIDYVVKKAVA
                     CLIEGQPDVSNLYPVFTGAPYPN"
BASE COUNT          234 a          475 c          464 g          215 t
ORIGIN      
        1 ggcaatggac caccgccacc cggtggcggg ccaaaagccc caggtcccgg gcgtcggcca
       61 cgttaacgtg caaggccagg ttcagcttgg gaagccagcc ggcggcgccc aggaaaccca
      121 cgggcgtggt gccgtggggc agggtccaat ccggattgag ggcctggatg cgcttggcca
      181 tggggccccg gcccgtcctg aagaaccggc cctcgtcccg gctttcgccc acgtggatcg
      241 tggtggggca atggggatgg gccgcggcga aacgcccggc caggagcagg agttccttcg
      301 acaccgtgaa gggcgtgtgg gggctgatgc cccagcggaa ggtctcgcgg ccctcgccgg
      361 cggccgtcag ggccttgagc ctctcctgga accctttcca aacggccttg gggtccggct
      421 gggccacgtc caaaagttcc aggaaggcga aggcccgcag gcccgagccc ggcagcagct
      481 tgaaactttc cccgctggtg gagatgtcgc acagcgtggt ggtgccctgg gccaggcttt
      541 cccggatgcc cgcctggacc gaccggtggt actccgccgc cgtggcggcg cggcccttca
      601 cggccatctc ccgcagccat tcgcggaaac tgcccttgaa aggcacgcgg cccttgaagg
      661 ccgtgtagtc caggtggcag tgggcgttga tgaggccggg caaaagcagg gtgtcgccca
      721 ggtccaacac gcgcaaggga cggggcgcgt ggaagtcctt gcggcgtccc acctgcagga
      781 tgcggctccc ctccaccaac acgaaggcgt cctccagggg ttgggggcgg tccagggtga
      841 gcagggtctt ggcgaagagc acgagtcgcg cgggcatggc cctaaaataa catggcccct
      901 ccccgccacc catggaattc ggcctttcgg gggcggcctt gacccctgtc cggggcgaag
      961 tgataatatc gggcgcccga aaagcgccaa accatccgcc gtccaggggc ccatgctcga
     1021 caataaattc caaaagttcc tcttccacct gaccagcctg gccaccaccg ctttcccccg
     1081 gggcggggcc tggttcttca accacaccat cccgcccatc ctgggcaacc gcgcgctggt
     1141 ggattggagc ttccggagga acctggcggc cctgcgcaag gtgcagaagt tcgagcgcat
     1201 cctggtcatc ccggacgtgc acatcggcga cgccatcatg atgcaggcgg ccgtgaccgc
     1261 cttccgcgat ttcttccccc aggcccgcat cgactacgtg gtgaagaagg ccgtggcctg
     1321 cctcatcgag ggccaaccgg acgtgtcgaa cctctacccg gtcttcacgg gcgcgcccta
     1381 ccccaacg
//
LOCUS       DASTJJ010000886         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_121685, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000886 DASTJJ010000000
VERSION     DASTJJ010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121685"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1080
                     /locus_tag="VFR02_10730"
     CDS             <1..>1080
                     /locus_tag="VFR02_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9870954.1"
                     /translation="HGGNFTVVAGERVEQELVWYPSHRTVPRDINLDSALASTLDYWN
                     DWAAKYNQDGKYAHLVKRSMLVLRALTHEDTGGIVAAPTTSLPEAFGGPRNWDYRFCW
                     LRDAALTLEAMLTHGYEKVALKWRDWLLRAVAGDPEDLQIMYGVGGERDLPEAVLDHL
                     PGYEGAGPVRIGNGAVGQYQADVVGEVLVALEKLRRAGGEEDHFSWPLQLAMLKFVER
                     HLADKDHGIWEMRGDLQHFTHSRVMMWAAFDCGVRAVRDHGLTGPVERWEELCGQLRA
                     EIMENGFNAELNSFTQTYGGTATDASLLVIPQVGFVAYDSPEMLGTVAALEKELVDSH
                     GMILRYETKFGLDGLAPGEHPFLACS"
BASE COUNT          183 a          342 c          380 g          175 t
ORIGIN      
        1 gcacggcggc aatttcacgg tggttgccgg cgaacgggtg gagcaggaac tggtctggta
       61 cccctcccac cggaccgtcc cgcgtgacat caacctggac tccgccctgg cctcgacgct
      121 tgactactgg aacgactggg cggccaagta caaccaggac ggcaagtatg cccacctcgt
      181 gaaacgttcc atgctggtgc tgcgggcact gacacacgag gacacaggcg ggattgtggc
      241 ggcccccacc acctcgcttc ctgaggcctt tggcgggccc cgcaactggg actaccgttt
      301 ttgctggttg cgcgacgccg ccctgacgct ggaggccatg ctcacgcacg gctacgaaaa
      361 agtggccctg aagtggcgcg actggctgct gcgcgccgtg gccggggacc ccgaggacct
      421 gcagatcatg tacggggtgg gaggggaacg tgacttgccc gaggcggtcc tggaccacct
      481 gcccggctac gagggcgccg gccccgtccg cattggcaac ggggccgtgg gacagtacca
      541 ggccgacgtc gtcggtgaag tcctggtggc gctggagaag ctgcggcggg ccggcggcga
      601 ggaagaccac ttctcctggc ccctgcaact ggccatgctg aagtttgtgg agcggcacct
      661 ggccgacaag gaccatggca tctgggagat gcgcggcgac ctccagcact tcacccattc
      721 acgggtcatg atgtgggcgg cgtttgactg cggcgtccgc gccgtccggg accacgggct
      781 gacggggccc gtggagcggt gggaggaact ctgcgggcag ctgcgggccg aaatcatgga
      841 aaacggcttc aacgccgaac tcaacagctt tacccagacc tacggcggca cggccaccga
      901 cgcctcgctg ctggtcattc cgcaggtggg gtttgtggcc tacgattcgc cggagatgct
      961 cggcaccgtg gcggcgttgg agaaggagct cgtggacagc cacgggatga tcctgcgcta
     1021 cgagacgaag tttgggctgg acggactggc cccgggggag caccccttcc tggcctgctc
//
LOCUS       DASTJJ010000887         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_366688, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000887 DASTJJ010000000
VERSION     DASTJJ010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366688"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="ftsZ"
                     /locus_tag="VFR02_10735"
     CDS             complement(<1..701)
                     /gene="ftsZ"
                     /locus_tag="VFR02_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9870955.1"
                     /translation="MIEFAEDDVTQYLAHLKVIGVGGGGCNAINKMIEAGIEGVECIS
                     ANTDYKSLRRSKAAKKIQLGKSLTKGLGSGGNPSIGKDAAEESVNEIHQALEGADMVV
                     VACGMGGGTGTGAAPVVAEVSKKMGILTVGVVSRPFEFEGPVRKAQAEDGLKAMLPHV
                     DAIVVIPNERINLVCEQDVDLTSAFSRVDDVLVQAIQGISVVIHSEGIINRDFADVKR
                     ILTAKGKVLMGTGYGT"
     gene            complement(1014..>1761)
                     /gene="ftsA"
                     /locus_tag="VFR02_10740"
     CDS             complement(1014..>1761)
                     /gene="ftsA"
                     /locus_tag="VFR02_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9870956.1"
                     /translation="VRSVERAGVTVNDIVLQPLASAEAILSADEKELGVVLIDIGGGT
                     TDIAVFINGSLWHTGIITLGGALVTSDVAVGLRTPNTEAESIKTQFGSAYTAMVKEEE
                     EITVPGVGGRPDRRMPRRVLSEIIEARMEEIFELVAAELKKHGFEDRVPAGAVITGGA
                     ALMEGTAELAEKILQLPVRIGSPKRVGGLTDVVSNPMYSTAVGLVLMGLKSNPSPSSG
                     SARRGAGPAPGGGVLQDILGRMKSWFGDSI"
BASE COUNT          280 a          643 c          505 g          333 t
ORIGIN      
        1 gtcccgtagc ccgtgcccat caacaccttg cccttggcgg tcaggatgcg cttcacgtcc
       61 gcgaagtcgc ggttgatgat gccctccgag tggatgacca ccgagatgcc ctggatggcc
      121 tgcaccagga cgtcgtcgac ccggctgaag gccgaggtca ggtccacgtc ctgctcgcac
      181 accaggttga tgcgttcgtt ggggatgacc acgatggcgt ccacgtgggg cagcatggcc
      241 ttcaggccgt cctcggcctg ggccttgcgc accggcccct cgaactcgaa gggccggctg
      301 accacgccga cggtcaggat ccccatcttc ttggacacct cggccaccac gggggccgcg
      361 cccgtgcccg tgccgccgcc catgccgcag gccaccacca ccatgtcggc gccttccagc
      421 gcctggtgga tctcgttcac cgactcctcg gcggcgtcct tgccgatcga ggggtttccg
      481 cccgagccca ggcccttggt gagggacttg cccaactgga tcttcttggc ggccttgctg
      541 cgccgcagcg acttatagtc ggtgttggcg gagatgcact ccacgccctc gatgccggcc
      601 tcgatcatct tattgatggc gttgcatccg ccgccgccca ccccgatgac cttcaggtgc
      661 gccaagtact gcgtcacgtc gtcttccgcg aactcgatca taaatccccc tcatcttgat
      721 ttttttggtg aaactcgacc ccattttttg cggctaagtg cggggacggg agcctcccgc
      781 caaaagcgcg atggctcgcg cccccacacc cctcaaccct ttcgttcgat cgccaaacaa
      841 tcaccacctt tccaaaatca aaccaacttc gaacctaaac ccctttcacc acaagcctcc
      901 ttcgggggcc acaaagttca cgaagtaaac ccaaattctt tcttttattt tcaatcctta
      961 cttcgtgccc ttcgtgtgcc gcgaagcggc atcgtggtgg caattgatgt tcttcagatc
     1021 gaatccccga accagctctt catgcggccc aggatgtcct gcaacacccc gccgcccggc
     1081 gcgggacccg cgccccgcct ggccgatccc gaggaaggcg aggggttcga cttcaacccc
     1141 atcaggacca ggcccacggc ggtggagtac atggggttcg acaccacgtc ggtcaagccc
     1201 cccacccgct tgggcgaccc gatgcgcacg ggcagctgca ggatcttctc ggccagctcg
     1261 gccgtgcctt ccatcaaggc ggcccccccg gtgatgacgg cgccggcggg cacccggtcc
     1321 tcgaacccgt gcttcttgag ctcggcggcc accagctcga agatctcctc catgcgggcc
     1381 tcgatgatct cggacagcac ccggcggggc atgcggcggt cgggccgccc ccccacgccg
     1441 gggaccgtga tctcctcctc ctccttcacc atggcggtgt aggcgctgcc gaactgggtc
     1501 ttgatgctct cggcctcggt gttgggcgtg cgcaggccta cggccacgtc gctggtgacc
     1561 aaggcgccgc ccagggtgat gatgcccgtg tgccagagcg acccgttgat gaacaccgcg
     1621 atgtccgtgg tgcccccccc gatgtcgatc agcaccacgc ccagttcctt ctcgtcggcg
     1681 gacaggatgg cctccgcgga ggccaggggc tgcagcacga tgtcgttgac cgtgaccccc
     1741 gcccgctcca cgctgcgcac g
//
LOCUS       DASTJJ010000888         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1344909, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000888 DASTJJ010000000
VERSION     DASTJJ010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344909"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1188
                     /locus_tag="VFR02_10745"
     CDS             <1..>1188
                     /locus_tag="VFR02_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9870957.1"
                     /translation="HGKLLGFDKPFLAPLTREVGKLLGGVYPELTARQDHIVNVVKAE
                     EERFYETLAAGTERLMEKIDGLLKAKASTLSGEDAFTLYDTYGFPLDITKEILREKGL
                     SVDEAGFQAAMEGQKEKARSAWKGSGDAALPPLYQEMEAKLGPSAFKGYDKTQDLGTV
                     AFLTRVKDKKHEAVEALQEGEYGFVVLNQTPFYAEKGGQVGDKGALKGFNSAEVSWAD
                     VLDTQMLTEKLIGHWVRQTRGKLSMGQKVEATVEESERRATMRNHTATHLLHAALRRV
                     LGTHVGQAGSYVGPDRLRFDFTHFAALTPEQVRAIERLVNQQVLDNVEVTKQEMTFDQ
                     AKQKGALAFFSEKYGEKVRVVDVPGFSMELCGGTHVGRTGDIGLFKVLAESSVAAGVR
                     RIEA"
BASE COUNT          229 a          373 c          412 g          174 t
ORIGIN      
        1 catgggaagc tgttgggttt cgacaagccc ttcctggccc ccctcacccg ggaagtgggg
       61 aagctgttgg ggggcgttta tcccgaactc accgcccgcc aggaccacat cgtgaacgtg
      121 gtgaaggccg aggaggaacg cttttacgag accctggcgg ccggtaccga gcgcctgatg
      181 gaaaagatcg acggcctgct gaaagccaaa gcctccaccc tttcggggga ggacgccttc
      241 accctctacg acacctacgg gttccccttg gacatcacca aggagatcct gcgcgagaag
      301 ggtttgtcgg tggatgaggc gggattccag gcggccatgg aggggcaaaa ggaaaaggcc
      361 cgctcggctt ggaaggggag cggcgacgcc gccctgcccc cgctctacca ggagatggag
      421 gccaagctcg gcccctcggc cttcaagggc tacgacaaga cccaggacct gggcacggtg
      481 gccttcctga cccgcgtgaa ggacaagaaa cacgaggcgg tggaggccct ccaggagggc
      541 gagtacggtt tcgtggtgct gaaccagacc cccttctacg ccgagaaggg cgggcaggtg
      601 ggcgacaagg gggccctgaa gggcttcaat tccgccgagg tgtcctgggc ggacgtgctg
      661 gacacccaga tgctcaccga gaagctcatc ggccattggg tgcggcagac ccgcggcaag
      721 ctgtcgatgg gccagaaggt ggaggccacg gtcgaggaga gcgagcggcg ggccaccatg
      781 cgcaaccaca ccgccaccca cctgctgcac gcggccctgc gccgggtgtt gggcacccac
      841 gtgggccagg ccggcagtta cgtgggcccg gaccgcctgc gtttcgactt cacccatttc
      901 gccgccctca cccccgagca ggtgcgcgcc atcgagcgcc tggtgaacca gcaggtgctg
      961 gacaacgtgg aagtgaccaa acaggaaatg accttcgacc aggccaaaca gaagggggcc
     1021 ctggccttct tcagcgagaa atacggcgag aaggtgcggg tggtggacgt gcccggcttt
     1081 tccatggaac tgtgcggcgg cacccacgtg ggccgcacgg gcgacatcgg gctcttcaag
     1141 gtgctggccg aatcctcggt ggccgccggc gtgcgccgca tcgaggcc
//
LOCUS       DASTJJ010000889         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1100288, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000889 DASTJJ010000000
VERSION     DASTJJ010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100288"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFR02_10750"
     CDS             complement(<1..374)
                     /locus_tag="VFR02_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET9870958.1"
                     /translation="MSGGLVLYDALLREGPQTQGINLSVKDKLRLAEAMDALGLHYIE
                     GGWPGANPKDDAFFKEAKKLKLKNARLSVFGMTRRAGRKAAQDPVLANLVKAGVPTVT
                     IFGKSWDFHVEHALKIPLQANL"
     gene            complement(371..>1531)
                     /locus_tag="VFR02_10755"
     CDS             complement(371..>1531)
                     /locus_tag="VFR02_10755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9870959.1"
                     /translation="ERIAKNRKAGHQVVVVVSAPGDTTDNLIEMARRISDEPDDREMD
                     VLLATGEQQSIALLAIALKSLGCDAISFTGPQVGIVTDDVHTKARIVKVGDSKIRKAL
                     NLGKVVIVAGFQGENKEHEITTLGRGGSDLTAVAIAAALKADVCEFYKDVDGVFTANP
                     GIVKKARKLPVISYDEMLEMASAGAQVLNARSVEFAKNFDIKIHVRPTFNEKEGTIVM
                     QETKAMEKVVISGVTYNKNEAKITVRGVPDRPGVASILFTKLGAAGVNVDMIIQNIGE
                     KGHSDVSFTVDKGEFKKAMKVVNEAAKVLKPAGVIADEDIAKVSVIGVGMRSHSGVAA
                     GMFKALAEEGVNIEMISTSEIKISVVIDQKDVAKAVNAIHKKFRLDKIGKRS"
BASE COUNT          213 a          503 c          489 g          326 t
ORIGIN      
        1 tccaggttcg cctgcagggg gatcttgagc gcgtgctcga cgtggaaatc ccagctcttg
       61 ccgaagatgg tgacggtggg gacgcccgcc ttgaccaggt tggccagcac ggggtcctgg
      121 gcggccttgc ggccggcccg gcgcgtcatg ccgaagaccg agaggcgcgc gttcttgagc
      181 ttgagcttct tggcctcctt gaagaaggcg tcgtccttgg ggttggcccc gggccagccg
      241 ccctcgatgt aatggagccc cagcgcgtcc atggcctcgg ccaggcgcag cttgtccttc
      301 accgacaggt tgatgccctg ggtctggggc ccttcccgca gcagcgcgtc gtagaggacc
      361 agcccgccgc tcaagaccgc ttcccgatct tgtcgaggcg gaacttcttg tggatggcgt
      421 tgacggcctt ggccacgtcc ttctggtcga tgaccaccga gatcttgatc tcggaggtgg
      481 agatcatctc gatgttgacc ccctcctcgg ccagggcctt gaacatgccg gcggccacgc
      541 ccgagtggga ccgcatgccc acgccgatga ccgagacctt ggcgatgtcc tcgtcggcga
      601 tgacgccggc gggtttgagc accttggcgg cctcgttcac caccttcatg gccttcttga
      661 actcgccctt gtccaccgtg aaggaaacgt ccgaatggcc cttttccccg atgttctgga
      721 tgatcatgtc cacgttcacg cccgcggcgc ccagcttggt gaagaggatg gaggccaccc
      781 cgggccggtc gggcacgccc cgcaccgtga tcttggcctc gttcttgttg taggtcaccc
      841 cgctgatgac caccttttcc atggctttgg tctcttgcat gacgatcgtc ccttcctttt
      901 cgttgaatgt gggccgcacg tggatcttga tgtcgaagtt cttggcgaat tccaccgagc
      961 gggcgttcag gacctgggcc cccgcgctgg ccatttccaa catctcatcg tagctgatga
     1021 cgggcagttt ccgggccttt ttcacgatgc ccgggttggc ggtgaagacc ccgtccacgt
     1081 ccttgtagaa ctcgcagacg tccgccttca gggcggccgc gatggccacc gccgtcaggt
     1141 cgctgccccc ccggcccagg gtggtgatct catgttcctt gttctcgccc tggaagccgg
     1201 ccacgatgac caccttgccc aggttgaggg ccttgcggat cttcgagtcc cccaccttga
     1261 cgatgcgcgc cttggtgtgg acgtcgtcgg tgacgatgcc cacctggggg cccgtgaagg
     1321 aaatggcgtc gcagcccagg ctcttcaggg cgatggccag gagcgcgatg gactgctgtt
     1381 ccccggtggc caggagcacg tccatttccc ggtcgtcggg ctcgtcggag atccgccggg
     1441 ccatctcgat gaggttgtcc gtggtgtcgc cgggggcgga caccaccacc accacctggt
     1501 ggccggcctt gcggttcttg gcgatgcgct c
//
LOCUS       DASTJJ010000890         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_1466703, whole
            genome shotgun sequence.
ACCESSION   DASTJJ010000890 DASTJJ010000000
VERSION     DASTJJ010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466703"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFR02_10760"
     CDS             <1..1197
                     /locus_tag="VFR02_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET9870960.1"
                     /translation="YDKIIAFGGRTLGEDAGPKYLNSPETLLFKKGETLYLLNAARDG
                     IRRAGFVLVVEGYFDALALHLHGFDNTVATLGTALTPQHGPLLKRYTKDVVFCYDADA
                     AGQAAVERGFDPLMQAGLAVKVLVLPDAKDPDEYLAKHPKEDLRALIDQAPDYFRWWA
                     ASLRKRMPEARKEDIIRALGDFVPTLAGLPDEGSVQAACAAIESELSLDNRDLLTLVN
                     TARKKGGPRRMPALDAPKPAAAPSKPPDGGSELEGDFLALLTEERGEFVPWAVNELSP
                     QYFGNARYRELFEKLGAGEMKSAELNELPELEPSFLRLEARAEKRKLDGALWKNQSPD
                     EVDLKERQIREAMILEFAAEIKKRWVKRELDSLTQKQSEAEKAGQVEQALDFARQKSE
                     LKKQFS"
     gene            1211..>1616
                     /locus_tag="VFR02_10765"
     CDS             1211..>1616
                     /locus_tag="VFR02_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HET9870961.1"
                     /translation="MKPAAGKTKSDHVNKILEVGKEKGYITYKQVNDILPDEVVSSEE
                     IDDILLMLGENDIKIVDQDEKAAAIPDDDKEVGATAASKDDDDDDEEDFDKPFETSGV
                     ESRMGDPVKMYLHEMGRISLLTREEEISIAKRI"
BASE COUNT          349 a          477 c          546 g          244 t
ORIGIN      
        1 tatgataaaa tcatcgcctt tggcggcagg acgctgggcg aggacgccgg acccaaatac
       61 ctcaattcgc ccgagaccct cctctttaaa aaaggtgaaa cgctctattt attgaacgcc
      121 gcgcgggatg ggatccggcg cgccggattc gtattagttg tggagggtta tttcgacgcc
      181 ctggccctgc acctccacgg cttcgacaac acggtggcca ccctggggac ggccctgacc
      241 ccccagcacg ggcctttgtt gaagcgttac acgaaggacg tggtcttctg ttatgacgcc
      301 gacgccgcgg gacaggcggc ggtcgagcgg gggttcgacc ccctgatgca ggcggggctc
      361 gcggtgaagg tgttggtgct gcccgacgcc aaggacccgg acgaatacct ggccaagcac
      421 ccgaaagagg accttcgggc cctcatcgac caggcgccgg actatttccg ctggtgggcg
      481 gcgtcgttga ggaagcggat gccggaggcg cgcaaagagg acatcatccg cgccctcggg
      541 gatttcgtgc ccaccctggc cgggctgccc gacgaggggt cggtgcaggc ggcctgcgcg
      601 gccatcgagt ccgagttgtc cctggacaac cgggacttgc tgaccctggt gaacaccgcc
      661 cgcaaaaagg gcggcccccg gcgcatgccg gccctggacg ccccgaagcc ggcggcggcg
      721 ccgtccaaac ccccggatgg gggttcggag ctggaaggcg actttttggc gttgttgacc
      781 gaggaacggg gcgagttcgt gccctgggcg gtgaacgagt tgtcgcccca gtatttcggc
      841 aacgcccgtt accgggagct gttcgagaag ctgggggcgg gcgagatgaa gagcgccgag
      901 ctcaacgagt tgcccgagct ggaaccgtcc tttttgcgcc tggaggcccg ggcggaaaaa
      961 cggaagctgg acggggcctt gtggaagaac cagtcccccg acgaggtgga cctgaaggaa
     1021 cggcagatcc gggaggccat gatcctggag ttcgccgccg agatcaagaa gcgctgggtg
     1081 aagcgggaac tggattccct gacccagaaa caaagcgagg cggaaaaggc cgggcaggtc
     1141 gaacaggccc tggatttcgc ccggcagaag agcgaactga agaaacaatt ctcctagaag
     1201 gcggcattgc atgaagccag cggctggaaa aacgaaatcc gaccacgtca ataagatcct
     1261 ggaagtgggc aaggaaaagg gctacatcac ctacaaacag gtgaacgaca tcctgcccga
     1321 cgaagtggtg tccagcgagg agatcgacga catcctgctc atgctgggtg agaacgacat
     1381 caagatcgtg gaccaggacg agaaggccgc cgccatcccg gacgacgaca aggaagtggg
     1441 cgccaccgcc gcctccaagg acgacgatga cgacgacgag gaggacttcg acaagccctt
     1501 cgagacctcg ggcgtggaaa gccgcatggg cgacccggtc aagatgtacc tgcacgagat
     1561 gggccgcatc tcgctgctca cccgcgagga ggagatctcc atcgccaagc gcatcg
//
LOCUS       DASTJJ010000891         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U1506 k141_855738, whole genome
            shotgun sequence.
ACCESSION   DASTJJ010000891 DASTJJ010000000
VERSION     DASTJJ010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969979
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:18:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,155
            CDSs (total)                      :: 2,110
            Genes (coding)                    :: 2,096
            CDSs (with protein)               :: 2,096
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855738"
                     /isolate="SMAG_U1506"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VFR02_10770"
     CDS             <1..1092
                     /locus_tag="VFR02_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET9870962.1"
                     /translation="SQENLLHHLETSIAYTLKNRGGHGLPLIGHADWNDCLNLNCFST
                     EPNESFQTAGDVKGSKAESVMIAGLFLYAAGEMASLYRHMGKAADSDRIQKAYDGMLK
                     VVEEQAWDGEWYTRAYDAQGHPVGSKSCEEGKIFIESQGWCVLGGAGKDNGRARQALE
                     SVHRMLYTPNGIILQQPAYSTYHLELGEVTSYPPGYKENAGIFCHNNTWINLGWCLLG
                     EGDRALEYYLSICPSAKEEQIETYRSEPYVYAQMIAGRDAATPGEAKNSWLTGTAAWT
                     FLTVSQGLLGVKPDFEGLRIDPCVPKGWREFNVTRQYRGVEYRVRVTNPQGVSRGVKE
                     MKVDGKTVPGNVIPPAKDKKTVQVEVVLG"
     gene            1124..>1583
                     /locus_tag="VFR02_10775"
     CDS             1124..>1583
                     /locus_tag="VFR02_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9870963.1"
                     /translation="MRKKVMGVAWSFLLLALGSDAPLQAQTTPRFFLDRMVEVQGFAP
                     QAGDEAREAGRESALLRKLEAKAGTAAVRLPASPRFSPRPGERLWVFYGSACRGSAVV
                     KGLKAQKVPGPIPARAWFELDASSLPATVNGNGHWLERAVYLEARPSDKEP"
BASE COUNT          299 a          493 c          543 g          248 t
ORIGIN      
        1 tcccaggaga acctgctgca ccacctggag accagcatcg cctacacgct caagaaccgg
       61 ggcggccacg ggcttcccct gatcggccac gccgactgga acgactgcct gaacctcaac
      121 tgcttctcca ccgagcccaa cgagtccttc cagaccgccg gcgacgtgaa gggttccaag
      181 gccgagtcgg tcatgatcgc ggggctcttc ctttacgcgg ccggggagat ggcctccctc
      241 taccgccaca tgggcaaggc cgctgactcg gaccggatcc aaaaggccta tgacggcatg
      301 ctgaaggtgg tggaggagca ggcctgggac ggggaatggt acacccgggc ctacgacgcc
      361 caaggccacc cggtgggttc caagtcctgc gaggaaggca agatcttcat cgagagccag
      421 ggctggtgcg tcttgggcgg cgcggggaag gacaacggcc gggcgcggca ggcgttggag
      481 agcgtgcacc ggatgctcta tacgcccaac ggcatcatcc tgcagcagcc ggcctacagc
      541 acctaccacc tggaactggg ggaagtgacc agttatccgc cgggttataa agagaacgcc
      601 ggcatcttct gccacaacaa cacctggatc aacctgggct ggtgcctctt gggcgagggc
      661 gaccgggcgc tggaatatta cctgtccatc tgcccctcgg ccaaggaaga acagatcgag
      721 acctaccgca gcgaacccta tgtctacgcc cagatgatcg cgggccggga cgcggccacg
      781 ccgggtgagg ccaagaactc ctggttgacg ggtacggccg cctggacctt cttgaccgtc
      841 tcccagggcc tcttgggcgt gaaacccgac ttcgaggggc tgaggatcga cccctgcgtg
      901 ccgaagggat ggagggaatt caacgtgacc cgccagtacc ggggagtgga ataccgggtg
      961 agggtcacga acccccaggg cgtttcccgg ggcgtgaagg aaatgaaggt ggacggcaag
     1021 accgtccccg gcaacgtcat tccgcccgcg aaggacaaga agaccgtgca ggtggaagtg
     1081 gtcctgggtt gaccaccggg gatttcagaa gggcggcgtg aggatgcgaa agaaagtcat
     1141 gggtgtggcc tggtcgttcc tcctcttggc cttgggatcg gacgcgccgc tccaggccca
     1201 aaccaccccg cggttcttcc tggaccggat ggtcgaggtg cagggtttcg cgccccaggc
     1261 gggcgatgaa gcccgcgagg cggggcggga atcggccttg ctgcgcaaac tcgaggcgaa
     1321 ggccgggacg gccgccgtaa ggcttccggc ttccccccgc ttttcccccc ggcctgggga
     1381 acgtctctgg gtcttttacg gttcggcctg ccggggcagc gcggtggtga agggactgaa
     1441 ggcccaaaag gtgccgggac cgatcccggc gcgggcctgg ttcgagctgg acgcctcgtc
     1501 gctgcccgcc acggtgaacg gcaacggcca ttggctggag cgggcggtct atctggaagc
     1561 ccggccttcg gataaggaac ccg
//
